Bookedited by Chhandak Basu.
Summary: "This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design , and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls." -- Provided by publisher.
Contents:
Fast masking of repeated primer binding sites in eukaryotic genomes / Reidar Andreson, Lauris Kaplinksi, and Maido Remm
Primer design for PCR reactions in forensic biology / Kelly M. Elkins
Design of primers and probes for quantitative real-time PCR methods / Alicia Rodríguez, Mar Rodríguez, Juan J. Córdoba, and María J. Andrade
Large-scale neucleotide sequence alignment and sequence variability assessment to identify the evolutionarily highly conserved regions for universal screening PCR assay design: an example of influenza A virus / Alexander Nagy, Tomáš Jiřinec, Lenka Černíková, Helena Jiřincová, and Martina Havlíčková
Low-concentration initiator primers improve the amplification of gene targets with high sequence variability / Kenneth E. Pierce and Lawrence J. Wangh
Multiplex PCR primer design for simultaneousu detection of multiple pathogens / Wenchao Yan
Degenerate primer design for highly variable genomes / Kelvin Li, Susmita Shrivastava, and Timothy B. Stockwell
Allele-specific real-time polymerase chain reaction as a tool for urate transporter 1 mutation detection / Juliet O. Makanga, Antonius Christianto, and Tetsuya Inazu
MultiPLX: automatic grouping and evaluation of PCR primers / Lauris Kaplinski and Maido Remm
In silico PCR primer designing and validation / Anil Kumar and Nikita Chrodia
Primer design using Primer Express® for SYBR green-based quantitative PCR / Amarjeet Singh and Girdhar K. Pandey
Designing primers for SNaPshot technique / Greciane Gaburro Paneto and Francisco de Paula Careta
Rapid and simple method of qPCR primer design / Brenda Thornton and Chhandak Basu
PRIMEGENSw3: a web-based tool for high-throughput primer and probe design / Garima Kushwaha, Gyan Prakash Srivastava, and Dong Xu
Selecting specific PCR primers with MFEprimer / Wubin Qu and Chenggang Zhang.