Bookedited by Duarte Miguel F. Prazeresl, Sofia Aires M. Martins.
Contents:
G protein-coupled receptors : an overview of signaling mechanisms and screening assays / Duarte Miguel F. Prazeres and Sofia Aires M. Martins
Time-resolved FRET strategy to screen GPCR ligand library / Nadia Oueslati [and others]
Homogeneous fluorescence anisotropy-based assay for characterization of ligand binding dynamics to GPCRs in budded baculoviruses : the case of Cy3B-NDP-α-MSH binding to MC4 receptors / Santa Veiksina . . [and others]
Construction of recombinant HEK293 cell lines for the expression of the neurotensin receptor NTSR1 / Su Xiao, Joseph Shiloach, and Reinhard Grisshammer
cAMP assay for GPCR ligand characterization : application of BacMam expression system / Olga Mazina [and others]
Ca2+ mobilization assays in GPCR drug discovery / Grzegorz Woszczek and Elisabeth Fuerst
Using constitutive activity to define appropriate high- throughput screening assays for orphan G protein-coupled receptors / Tony Ngo, James L.J. Coleman, and Nicola J. Smith
Monitoring G protein-coupled receptor activation using the protein fragment complementation technique split TEV / Michael C. Wehr, Sabrina Galinski, and Moritz J. Rossner
Quantifying GPCR iternalization : a focus on the kisspeptin receptor / Macarena Pampillo and Andy V. Babwah
GPCR oligomerization analysis by means of BRET and dFRAP / Francisco Ciruela and Víctor Fernández-Dueñas
Use of imageJ to recover information from individual cells in a G protein-coupled receptor assay / João R.C. Trabuco, Sofia Aires M. Martins, and Duarte Miguel F. Prazeres
Methods to immobilize GPCR on the surface of SPR sensors / Laura Martínez-Muñoz [and others]
Olfactory receptor screening assay using nanovesicle- immobilized carbon nanotube transistor / Jong Hyun Lim [and others]
Label-free biosensor assays in GPCR screening / Manuel Grundmann and Evi Kostenis
Multidimensional GPCR profiling and screening using impedance-based label-free and real-time assay / Ning Ke [and others]
Label-free functional selectivity assays / Ann M. Ferrie [and others]
Measurement of surface-mediated Ca2+ transients on the single-cell level in a microfluidic lab-on-a-chip environment / Michael Kirschbaum, Magnus S. Jaeger, and Claus Duschl
Cell-based assays and animal models for GPCR drug screening / Hideo Takakura [and others]
Computer-aided design of GPCR ligands / Hugo Gutiérrez-de-Terán [and others].