Bookedited by Shondra M. Pruett-Miller.
Contents:
Genome editing by targeted chromosomal mutagenesis
Piggybac transposon-based insertional mutagenesis in mouse haploid embryonic stem cells
Using phage integrases in a site-specific dual integrase cassette exchange strategy
Therapeutic genome mutagenesis using synthetic donor DNA and triplex-forming molecules
Genome engineering using adeno-associated virus (AAV)
Engineering of customized meganucleases via in vitro compartmentalization and in cellulo optimization
Efficient design and assembly of custom TALENs using the golden gate platform
Ligation-independent cloning (LIC) assembly of TALEN genes
Assembly and characterization of megatals for hyperspecific genome engineering applications
Genome engineering using CRISPR-Cas9 system
Donor plasmid design for codon and single base genome editing using zinc finger nucleases
Endogenous gene tagging with fluorescent proteins
Silencing long noncoding RNAs with genome-editing tools
Gene editing using ssODNs with engineered endonucleases
Genome editing in human pluripotent stem cells using site-specific nucleases
Strategies to increase genome editing frequencies and to facilitate the identification of edited cells
Using engineered endonucleases to create knockout and knockin zebrafish models
Creating knockout and knockin rodents using engineered endonucleases via direct embryo injection
Simple sperm preservation by freeze-drying for conserving animal strains.