Bookedited by Andreas Werner.
Contents:
Targeted small noncoding rna-directed gene activation in human cells
Isolation of chromatoid bodies from mouse testis as a rich source of short RNAs
Generation of endo-siRNAs in xenopus laevis oocytes
Analysis of endo-siRNAs in drosophila
Methods for studying the biological consequences of endo-siRNA deficiency in drosophila melanogaster
Small RNA library cloning procedure for deep sequencing of specific endogenous siRNA classes in caenorhabditis elegans
Assays for direct and indirect effects of c. Elegans endo-siRNAs
Extraction and nonradioactive detection of small RNA molecules
P19-mediated enrichment and detection of siRNAs
Detection of small noncoding RNAs by in situ hybridization using probes of 2'-o-methyl RNA + LNA
Enhanced detection of small RNAs using a nonradioactive approach
Computing siRNA and piRNA overlap signatures
Isolation of small interfering RNAs using viral suppressors of RNA interference
Computational analysis, biochemical purification, and detection of tRNA-derived small RNA fragments
Differential DNA methylation patterns in endo-siRNAs mediated silencing of LINE-1 retrotransposons.