Bookedited by Russell J. Diefenbach, Cornel Fraefel.
Contents:
HSV-1 biology and life cycle / Roger D. Everett
Herpes simplex virus growth, preparation, and assay / Peggy Marconi and Roberto Manservigi
Isolation of herpes simplex virus nucleocapsid DNA / Moriah Szpara
Construction and characterization of bacterial artificial chromosomes (BACs) containing herpes simplex virus full-length genomes / Claus-Henning Nagel, Anja Pohlmann, and Beate Sodeik
Engineering HSV-1 vectors for gene therapy / William F. Goins [and three others]
Herpes simplex virus type 1 (HSV-1)-derived amplicon vectors / Matias E. Melendez, Cornel Fraefel, and Alberto L. Epstein
HSV-1 amplicon vectors as genetic vaccines / Andrea S. Laimbacher and Cornel Fraefel
Modification of HSV-1 to an oncolytic virus / Hiroshi Nakashima and E. Antonio Chiocca
Herpes simplex virus mutant generation and dual-detection methods for gaining insight into latent/lytic cycles in vivo / Nancy M. Sawtell and Richard L. Thompson
Phenotypic and genotypic testing of HSV-1 resistance to antivirals / Andreas Sauerbrei and Kathrin Bohn
Using homogeneous primary neuron cultures to study fundamental aspects of HSV-1 latency and reactivation / Ju Youn Kim [and four others]
Characterization of extracellular HSV-1 virions by proteomics / Roger Lippé
Precipitation-based assay to analyze interactions of viral particles with cytosolic host factors / Kerstin Radtke, Fenja Anderson, and Beate Sodeik
Affinity purification combined with mass spectrometry to identify herpes simplex virus protein-protein interactions / David G. Meckes Jr.
Preparation of herpes simplex virus-infected primary neurons for transmission electron microscopy / Monica Miranda-Saksena, Ross Boadle, and Anthony L. Cunningham
Multifluorescence live analysis of herpes simplex virus type-1 replication / Michael Seyffert [and three others]
Expression, purification, and crystallization of HSV-1 glycoproteins for structure determination / Samuel D. Stampfer and Ekaterina E. Heldwein
Cryo-EM techniques to resolve the structure of HSV-1 capsid-associated components / Ryan H. Rochat, Corey W. Hecksel, and Wah Chiu
In vivo HSV-1 DNA transport studies using murine retinal ganglion cells / Jolene M. Draper, Graham S. Stephenson, and Jennifer H. LaVail
HSV-1 protein expression using recombinant baculoviruses / Lorry M. Grady, Ping Bai, and Sandra K. Weller
Murine intravaginal HSV-2 challenge model for investigation of DNA vaccines / Joshua O. Marshak, Lichun Dong, and David M. Koelle.