Bookedited by David J. Carroll, Stephen A. Stricker.
Contents:
Culturing larvae of marine invertebrates / Richard R. Strathmann
Obtaining gametes and embryos of ascidians / Charles C. Lambert
Artificial seawater culture of the gastropod Crepidula fornicate for studies of larval settlement and metamorphosis / Anthony Pires
Trichoplax adhaerens, an enigmatic basal metazoan with potential / Andreas Heyland, Roger Croll, Sophie Goodall, Jeff Kranyak, and Russell Wyeth
Rearing gymnolaemate bryozoan larvae for cellular and molecular analyses / Michael H. Temkin
An invertebrate embryologist's guide to routine processing of confocal images / George von Dassow
TEM analyses of chaetognath reproductive organs / George L. Shinn
SEM analysis of marine invertebrate gametes / John Buckland-Nicks
Imaging neural development in embryonic and larval sea urchins / Oliver Krupke, Shunsuke Yaguchi, Junko Yaguchi, and Robert D. Burke
Preparation and use of sea urchin egg homogenates / Anthony J. Morgan and Antony Galione
Microinjection and 4D fluorescence imaging in the eggs and embryos of the ascidian Phallusia mammillata / Alex McDougall, Karen Wing-man Lee, and Remi Dumollard
Isolating specific embryonic cells of the sea urchin by FACS / Celina Juliano, S. Zachary Swartz, and Gary Wessel
Biotinylation of oocyte cell surface proteins of the starfish Patiria miniata / Samantha Cihal and David J. Carroll
Perturbations to the hedgehog pathway in sea urchin embryos / Jacob F. Warner and David R. McClay
Regulation of DNA synthesis at the first cell cycle in the sea urchin in vivo / Jolanta Kisielewska and Michael Whitaker
Immunoblotting analyses of changes in protein phosphorylations during oocyte maturation in marine nemertean worms / Jose R. Escalona and Stephen A. Stricker
Multicolor labeling in developmental gene regulatory network analysis / Aditya J. Sethi, Robert C. Angerer, and Lynne M. Angerer
A 96-well plate format for detection of marine zooplankton with the sandwich hybridization assay / Julio B.J. Harvey
Isolation and assessment of signaling proteins from synchronized cultures during egg activation and through the egg-to-embryo transition in sea urchins / Michelle M. Roux-Osovitz and Kathy R. Foltz
Tools for sea urchin genomic analysis / R. Andrew Cameron
Antibody inhibition of protein activity in starfish oocytes / Eiichi Okumura, Masatoshi Hara, and Takeo Kishimoto
Immunophotoaffinity labeling of the binding proteins for 1-methyladenine, an oocyte maturation-inducing hormone of starfish / Tetsuo Kida, Shinjiro Matsuda, Atsushi Kuyama, and Tetsuo Toraya.