Bookedited by Mihaela Gadjeva.
Contents:
Overview / Mihaela Gadjeva
Evaluation of complement function by ELISA / Anja Roos and Jörgen Wieslander
Classical complement pathway component C1q : purification of human C1q, isolation of C1q collagen-like and globular head fragments and production of recombinant C1q-derivatives. Functional characterization / Mihaela Kojouharova
Classical complement pathway components C1r and C1s : purification from human serum and in recombinant form and functional characterization / Véronique Rossi ... [et al.]
Expression and purification methods for the production of recombinant human complement component C2 / Angela W. Norton ... [et al.]
Purification and characterization of human and mouse complement C3 / Marieta M. Ruseva and Meike Heurich
Purification of human complement protein C5 / Lars Sottrup-Jensen and Gregers Rom Andersen
Quantification of complement C5b-9 binding to cells by flow cytometry / Oren Moskovich and Zvi Fishelson
Challenges and opportunities in fractionation of recombinant human mannan-binding lectin / Thomas Vorup-Jensen
Genotyping of FCN and MBL2 polymorphisms using pyrosequencing / Lea Munthe-Fog, Hans Ole Madsen, and Peter Garred
Assay for estimation of the functional activity of the mannan-binding lectin pathway of the complement system / Troels R. Kjaer and Steffen Thiel
Purification, measurement of concentration, and functional complement assay of human ficolins / Misao Matsushita ... [et al.]
Purification and functional analysis of human properdin / Joseph O'Flynn ... [et al.]
Purification and functional characterization of C4b- binding protein (C4BP) / Frida C. Mohlin and Anna M. Blom
Purification and functional characterization of factor I / Sara C. Nilsson and Anna M. Blom
C1 inhibitor : quantification and purification / Lilian Varga and József Dobó
Purification, quantification, and functional analysis of complement factor H / Bing-Bin Yu ... [et al.]
Factor H-related proteins / Mihály Józsi and Seppo Meri
Functional evaluation of factor H genetic and acquired abnormalities : application for atypical hemolytic uremic syndrome (aHUS) / Lubka T. Roumenina ... [et al.]
Anti-factor H autoantibodies assay / Marie-Agnès Dragon-Durey ... [et al.]
Purification of native surfactant protein SP-A from pooled amniotic fluid and bronchoalveolar lavage / Najmunisa Karbani ... [et al.]
Purification of surfactant protein D (SP-D) from pooled amniotic fluid and bronchoalveolar lavage / Eswari Dodagatta-Marri ... [et al.]
Functional analysis of C5a effector responses in vitro and in vivo / Christian M. Karsten, Yves Laumonnier, and Jörg Köhl
Detection of complement receptors 1 and 2 on mouse splenic B cells using flow cytometry / Luke R. Donius and John H. Weis
Quantification of complement receptor 2 calcium signaling enhancement using flow cytometry / Luke R. Donius and John H. Weis
Purification of C1q receptors and functional analysis / Berhane Ghebrehiwet and Ellinor I.B. Peerschke
Detection of cell membrane-bound CD46 using flow cytometry / Martin Kolev and Claudia Kemper
Detection of CD59 using flow cytometry / Martin Kolev
Functional analysis of CD59 using complement-dependent cytotoxicity assay / Martin Kolev
Detection of soluble CR3 (CD11b/CD18) by time-resolved immunofluorometry / Gitte Krogh Nielsen and Thomas Vorup-Jensen - Analysis of the complement activation in mice / Nirmal K. Banda and Kazue Takahashi
Opsonophagocytic assay / Markryan Dwyer and Mihaela Gadjeva.