Bookedited by Robert J. Henry, Agnelo Furtado.
Contents:
DNA Extraction from Vegetative Tissue for Next Generation Sequencing?
DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples)
RNA Extraction from Cereal Vegetative Tissue
RNA Extraction from Developing or Mature Wheat Seeds
cDNA Library Preparation
Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants
The Polymerase Chain Reaction (PCR)
General Methods
Mutation and Mutation Screening
The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression
Cloning of DNA Fragments: Ligation Reactions in Agarose Gel
Rapid Cloning of Genes and Promoters for Functional Analyses
Genome Walking
Functional Analysis by Protein Biochemistry
Genomic Southern Blot analysis
Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs
Protein Blotting-Protocol for Beginners
Genetic Transformation of Wheat via Particle Bombardment
Sorghum Genetic Transformation by Particle Bombardment
Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery
A Protocol for High-throughput Agrobacterium-mediated Barley Transformation
Agrobacterium-mediated Transformation- Rice Transformation
Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos
A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops
A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data.