Bookedited by Ray J. Rose.
Summary: Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.
Contents:
Model legume genomes / Steven B. Cannon
Fluorescent in situ hybridization (FISH) on pachytene chromosomes as a tool for genome characterization / René Geurts and Hans de Jong
Targeted mutagenesis for functional analysis of gene duplication in legumes / Shaun J. Curtin [and six others]
RNA-seq for transcriptome analysis in non-model plants / Rohini Garg and Mukesh Jain
Functional analysis of legume genome arrays / Nicolas Goffard and Georg Weiller
Genome-wide identification of microRNAs in Medicago truncatula by high-throughput sequencing / Tian-Zuo Wang and Wen-Hao Zhang
Determining abundance of microRNAs and other small RNAs in legumes / Cecilia Contreras-Cubas, Alejandra A. Covarrubias, and José Luis Reyes
Forward genetics screening of Medicago truncatula Tnt1 insertion lines / Juan Carlos Serrani Yarce [and four others]
Reverse genetics in Medicago truncatula using a TILLING mutant collection / Maria Carelli [and seven others]
High-throughput and targeted genotyping of Lotus japonicus LORE1 insertion mutants / Dorian Fabian Urbański [and three others]
Isolation and forward genetic analysis of developmental genes in pea / James L. Weller, Valérie F.G. Hecht, and Frances C. Sussmilch
Gene silencing in Medicago truncatula roots using RNAi / Daniela S. Floss [and four others]
Molecular markers for genetics and plant breeding : the MFLP marker system and its application in narrow-leafed lupin (Lupinus angustifolius) / Islam Shahidul, Huaan Yang, and Guijun Yan
Stable transformation of Medicago truncatula cv. Jemalong for gene analysis using Agrobacterium tumefaciens / Youhong Song, Kim E. Nolan, and Ray J. Rose
Transient gene expression in Medicago truncatula leaves via agroinfiltration / Kelsey Picard [and three others]
Subcellular localization of transiently-expressed fluorescent fusion proteins / David A. Collings
Proteomics and the analysis of nodulation / Nijat Imin
Phosphoproteomic analysis of peptides / Mark A. Baker
Plant metabolomics : from experimental design to knowledge extraction / Amit Rai, Shivshankar Umashankar, and Sanjay Swarup.