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  • Book
    edited by Yaron Shav-Tal.
    Summary: As imaging technologies and approaches have evolved, the scope of certain imaging techniques has moved far beyond the production of purely illustrative images or appealing time-lapse movies to providing the scientist with a rich range of ways to measure and quantify the biological process and outcome of gene expression. In Imaging Gene Expression: Methods and Protocols, expert authors offer up-to-date approaches and protocols that scientists in the field have developed, which would benefit the broader scientific community. Divided in three convenient parts, this detailed book covers the output of a gene, namely the RNA molecules that are transcribed from the gene and the way by which these molecules can be tracked or quantified in fixed or living cells, protocols that focus on the gene, DNA, or chromatin, as well as a variety of ways by which nuclear processes intertwined with gene expression can be followed and quantified in living cells as well as approaches for studying several sub-nuclear structures found in eukaryotic cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Imaging Gene Expression: Methods and Protocols will serve researchers working toward imaging in the context of complete organisms.

    Contents:
    High-throughput fluorescence-based screen to identify factors involved in nuclear receptor recruitment to response elements / Tina B. Miranda, Ty C. Voss, and Gordon L. Hager
    Live-cell imaging combined with immunofluorescence, RNA, or DNA FISH to study the nuclear dynamics and expression of the X-inactivation center / Tim Pollex, Tristan Piolot, and Edith Heard
    Single-molecule resolution fluorescent in situ hybridization (smFISH) in the yeast S. cerevisiae / Samir Rahman and Daniel Zenklusen
    Measuring transcription dynamics in living cells using fluctuation analysis / Matthew L. Ferguson and Daniel R. Larson
    Tracking nuclear Poly(A) RNA movement within and among speckle nuclear bodies and the surrounding nucleoplasm / Joan C. Ritland Politz and Thoru Pederson
    Nuclear trafficking and export of single, native mRNPs in Chironomus tentans salivary gland cells / Tim P. Kaminski ... [et al.]
    Single mRNP tracking in living mammalian cells / Alon Kalo, Pinhas Kafri, and Yaron Shav-Tal
    Imaging nascent RNA dynamics in dictyostelium / Jonathan R. Chubb ... [et al.]
    Monitoring dynamic binding of chromatin proteins in vivo by single-molecule tracking / Davide Mazza, Sourav Ganguly, and James G. McNally
    Single-particle tracking for studying the dynamic properties of genomic regions in live cells / Irena Bronshtein Berger, Eldad Kepten, and Yuval Garini
    Microscopic analysis of chromatin localization and dynamics in C. elegans / Christian Lanctôt and Peter Meister
    Measuring the dynamics of chromatin proteins during differentiation / Arigela Harikumar and Eran Meshorer
    Electron spectroscopic tomography of specific chromatin domains / Liron Even-Faitelson ... [et al.]
    BAC manipulations for making BAC transgene arrays / Nimish Khanna ... [et al.]
    Spatiotemporal visualization of DNA replication dynamics / Marius Reinhart, Corella S. Casas-Delucchi, and M. Cristina Cardoso
    Dynamics of DNA damage repair and transcription / Niraj M. Shanbhag and Roger A. Greenberg
    Fluorescence microscopy-based high-throughput screening for factors involved in gene silencing / Sebastian Bultmann and Heinrich Leonhardt
    Actin as a model for the study of nucleocytoplasmic shuttling and nuclear dynamics / Kari-Pekka Skarp and Maria K. Vartiainen
    Isochronal visualization of transcription and proteasomal proteolysis in cell culture or in the model organism, Caenorhabditis elegans / Anna von Mikecz and Andrea Scharf
    Considering discrete protein pools when measuring the dynamics of nuclear membrane proteins / Nikolaj Zuleger, David A. Kelly, and Eric C. Schirmer
    Correlative microscopy of individual cells : sequential application of microscopic systems with increasing resolution to study the nuclear landscape / Barbara Hübner, Thomas Cremer, and Jürgen Neumann
    Time-lapse, photoactivation, and photobleaching imaging of nucleolar assembly after mitosis / Danièle Hernandez-Verdun, Emilie Louvet, and Eleonora Muro
    Nucleation of nuclear bodies / Miroslav Dundr.High-throughput fluorescence-based screen to identify factors involved in nuclear receptor recruitment to response elements / Tina B. Miranda, Ty C. Voss, and Gordon L. Hager
    Live-cell imaging combined with immunofluorescence, RNA, or DNA FISH to study the nuclear dynamics and expression of the X-inactivation center / Tim Pollex, Tristan Piolot, and Edith Heard
    Single-molecule resolution fluorescent in situ hybridization (smFISH) in the yeast S. cerevisiae / Samir Rahman and Daniel Zenklusen
    Measuring transcription dynamics in living cells using fluctuation analysis / Matthew L. Ferguson and Daniel R. Larson
    Tracking nuclear Poly(A) RNA movement within and among speckle nuclear bodies and the surrounding nucleoplasm / Joan C. Ritland Politz and Thoru Pederson
    Nuclear trafficking and export of single, native mRNPs in Chironomus tentans salivary gland cells / Tim P. Kaminski ... [et al.]
    Single mRNP tracking in living mammalian cells / Alon Kalo, Pinhas Kafri, and Yaron Shav-Tal
    Imaging nascent RNA dynamics in dictyostelium / Jonathan R. Chubb ... [et al.]
    Monitoring dynamic binding of chromatin proteins in vivo by single-molecule tracking / Davide Mazza, Sourav Ganguly, and James G. McNally
    Single-particle tracking for studying the dynamic properties of genomic regions in live cells / Irena Bronshtein Berger, Eldad Kepten, and Yuval Garini
    Microscopic analysis of chromatin localization and dynamics in C. elegans / Christian Lanctôt and Peter Meister
    Measuring the dynamics of chromatin proteins during differentiation / Arigela Harikumar and Eran Meshorer
    Electron spectroscopic tomography of specific chromatin domains / Liron Even-Faitelson ... [et al.]
    BAC manipulations for making BAC transgene arrays / Nimish Khanna ... [et al.]
    Spatiotemporal visualization of DNA replication dynamics / Marius Reinhart, Corella S. Casas-Delucchi, and M. Cristina Cardoso
    Dynamics of DNA damage repair and transcription / Niraj M. Shanbhag and Roger A. Greenberg
    Fluorescence microscopy-based high-throughput screening for factors involved in gene silencing / Sebastian Bultmann and Heinrich Leonhardt
    Actin as a model for the study of nucleocytoplasmic shuttling and nuclear dynamics / Kari-Pekka Skarp and Maria K. Vartiainen
    Isochronal visualization of transcription and proteasomal proteolysis in cell culture or in the model organism, Caenorhabditis elegans / Anna von Mikecz and Andrea Scharf
    Considering discrete protein pools when measuring the dynamics of nuclear membrane proteins / Nikolaj Zuleger, David A. Kelly, and Eric C. Schirmer
    Correlative microscopy of individual cells : sequential application of microscopic systems with increasing resolution to study the nuclear landscape / Barbara Hübner, Thomas Cremer, and Jürgen Neumann
    Time-lapse, photoactivation, and photobleaching imaging of nucleolar assembly after mitosis / Danièle Hernandez-Verdun, Emilie Louvet, and Eleonora Muro
    Nucleation of nuclear bodies / Miroslav Dundr.
    Digital Access Springer 2013