Bookedited by Chris Gehring.
Summary: Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better understand the biological role of this important signaling system. Written in the highly successful Methods in Molecular Biology [trademark] series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cyclic Nucleotide Signaling in Plants: Methods and Protocols seeks to aid scientist in further understanding signal transduction and the molecular mechanisms underlying cellular signaling.
Contents:
Molecular methods for the study of signal transduction in plants / Helen R. Irving and Chris Gehring
Recombinant expression and functional testing of candidate adenylate cyclase domains / Oziniel Ruzvidzo ... [et al.]
Quantification of cyclic dinucleotides by reversed-phase LC-MS/MS / Heike Burhenne and Volkhard Kaever
Determination of ADP-ribosyl cyclase activity, cyclic ADP-ribose, and nicotinic acid adenine dinucleotide phosphate in tissue extracts / Richard M. Graeff and Hon Cheung Lee
In vivo imaging of cGMP in plants / Jean-Charles Isner and Frans J.M. Maathuis
Characterization of heterologously expressed transporter genes by patch- and voltage-clamp methods : application to cyclic nucleotide-dependent responses / Fouad Lemtiri-Chlieh and Rashid Ali
Noninvasive microelectrode ion flux estimation technique (MIFE) for the study of the regulation of root membrane transport by cyclic nucleotides / Natalia Maria Ordoñez ... [et al.]
Calcium imaging of the cyclic nucleotide response / Martin R. McAinsh, Stephen K. Roberts, and Lyudmila V. Dubovskaya
Identification and quantitation of signal molecule-dependent protein phosphorylation / Arnoud Groen ... [et al.]
Comparative gel-based phosphoproteomics in tesponse to signaling molecules / Claudius Marondedze, Kathryn Lilley, and Ludivine Thomas
Affinity pull-down approach to identify the plant cyclic nucleotide interactome / Lara Donaldson and Stuart Meier
Structural and functional characterization of receptor kinases with nucleotide cyclase activity / Victor Muleya, Janet I. Wheeler, and Helen R. Irving
Computational identification of candidate nucleotide cyclases in higher plants / Aloysius Wong and Chris Gehring
Identification of cyclic nucleotide gated channels using regular expressions / Alice K. Zelman, Adam Dawe, and Gerald A. Berkowitz
Inferring biological functions of guanylyl cyclases with computational methods / May Alqurashi and Stuart Meier
Identification and characterization of cyclic nucleotide phosphodiesterases / Erin B. Purcell and Rita Tamayo
Detection of reactive oxygen species downstream of cyclic nucleotide signals in plants / Robin K. Walker and Gerald A. Berkowitz
Measurement of nitric oxide in plant tissue using difluorofluorescein and oxyhemoglobin / Ndiko Ludidi
Infrared gas analysis technique for the study of the regulation of photosynthetic responses / Alex Valentine ... [et al.].