Bookedited by Bernice Wright, Che J. Connon.
Summary: Continuous regeneration of the cornea is necessary to maintain this tissue in the transparent state that is essential for vision. Therapy for repair of the damaged anterior cornea is currently addressed through the transplantation of donor corneas or the delivery of limbal epithelial stem cells (LESC) to the ocular surface using amniotic membrane (AM) as a supporting scaffold. Research on the bioengineering of corneal equivalents as replacement tissue is underway to develop viable corneal prosthetics. Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine. Divided into four convenient sections, topics include the identification, characterisation and cultivation of LESC, as well as the investigation of biopolymers used as the basis for corneal substitutes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Corneal Regenerative Medicine: Methods and Protocols covers the fundamental techniques useful for both the laboratory and clinical settings.
Contents:
Limbal epithelial cell therapy : past, present, and future / Tor Paaske Utheim
Artificial cornea / Naresh Polisetti, Mohammad Mirazul Islam, and May Griffith
Clonal analysis of limbal epithelial stem cell populations / Ursula Schlötzer-Schrehardt
Use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation / Ashley M. Crane and Sanjoy K. Bhattacharya
Enrichment of human corneal epithelial stem/progenitor cells by magnetic bead sorting using SSEA4 as a negative marker / Martin N. Nakatsu and Sophie X. Deng
Limbal epithelial stem cell identification using immunoblotting analysis / Bernice Wright and Che J. Connon
Culture of limbal epithelial cells / Tor Paaske Utheim, Torstein Lyberg, and Sten Ræder
Culture of limbal stromal cells and corneal endothelial cells / Naresh Polisetti and Nancy C. Joyce
Formation of a tissue-engineered cornea using plastically compressed collagen scaffolds and limbal stem cells / Shengli Mi and Che J. Connon
Fabrication of a human recombinant collagen-based corneal substitute using carbodiimide chemistry / Mohammad Mirazul Islam, May Griffith, and Kimberley Merrett
Fabrication of a corneal-limbal tissue substitute using silk fibroin / Laura J. Bray [and others]
Cultivation of limbal epithelial cells on electrospun poly(lactide-co-glycolide) scaffolds for delivery to the cornea / Pallavi Deshpande [and others]
Growth and delivery of mesenchymal and limbal stem cells using copolymer polyamide 6/12 nanofiber scaffolds / Vladimir Holan, Eliska Javorkova, and Peter Trosan
Encapsulation and culture of mammalian cells including corneal cells in alginate hydrogels / Nicola C. Hunt and Liam M. Grover
In vivo confocal microscopy of the cornea to assess tissue regenerative response after biomaterial implantation in humans / Neil Lagali, May Griffith, and Per Fagerholm.