Bookedited by Kimberly McCall, Charles Klein.
Summary: Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. -- Publisher website.
Contents:
Navigation to the graveyard-induction of various pathways of necrosis and their classification by flow cytometry / Christina Janko [and others]
Time-lapse imaging of necrosis / Fredrik Wållberg, Tencho Tenev, and Pascal Meier
Activity assays for receptor-interacting protein kinase 1 : a key regulator of necroptosis / Jenny L. Maki and Alexei Degterev
Fluorescent biosensors for the detection of HMGB1 release / Isabelle Martins [and others]
Methods for the study of entosis / Qiang Sun and Michael Overholtzer
Analysis of pyroptosis in bacterial infection / Lia Danelishvili and Luiz E. Bermudez
Analysis of cell death by electron microscopy / S. Burattini and E. Falcieri
Histological analysis of neurodegeneration in the mouse brain / Hiroo Yamaguchi and Jie Shen
Quantitation of acute necrosis after experimental myocardial infarction / Xin-Yi Yeap .. [et al.]
Immunohistological tools to discriminate apoptotic and necrotic cell death in the skin / Marion C. Bonnet
Identification and characterization of programmed cell death markers in bacterial models / Daniel J. Dwyer and Jonathan A. Winkler
Quantification of genetically controlled cell death in budding yeast / Xinchen Teng and J. Marie Hardwick
Necrosis in C. elegans / Matt Crook, Avni Upadhyay, and Wendy Hanna-Rose
Monitoring the clearance of apoptotic and necrotic cells in the nematode Caenorhabditis elegans / Zao Li [and others]
Modeling calcium-overload mediated necrosis in Drosophila / Kai Liu, Yuhong Li, and Lei Liu
Use of necrotic markers in the Drosophila ovary / Allison K. Timmons [and others]
Detection and measurement of necrosis in plants / Elena A. Minina [and others].