Bookedited by Fernando Vivanco.
Summary: Proteomics is a rapidly expanding investigation platform in cardiovascular medicine. Driven by major improvements in mass spectrometry (MS) instrumentation and data analysis, the proteomics field has flourished in recent years particularly in the study of complex diseases. These recent advances are characterized by the development of quantitative MS-based methods that promoted the field from primarily identifying proteins to also providing measurements of relative changes in protein levels between different cell states. This progress is reflected in the application of proteomic techniques to vascular pathology. Vascular Proteomics: Methods and Protocols provides up-to-date methods and protocols for the analysis of arteries, cells, lipoproteins, body fluids, and metabolites, with a particular focus on MS-based methods of protein and peptide quantification. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Vascular Proteomics: Methods and Protocols is a representative selection of methods that can be a useful resource for experienced proteomics practitioners as well as newcomers interested in becoming acquainted with the practice of proteomic techniques for cardiovascular research.
Contents:
Vascular Proteomics
Laser Microdissection and Saturation Labeling Dige Method for the Analysis of Human Arteries
Use of TOF-SIMS in Vascular Biology
Proteomic Analysis of Vascular Smooth Muscle Cells with S- and R-Enantiomers of Atenolol by ITRAQ and LC-MS/MS
Phosphoproteomic Analysis of Aortic Endothelial Cells Activated by Oxidized Phospholipids
Characterization of Membrane and Cytosolic Proteins of Erythrocytes
Characterization and Analysis of Human Arterial Tissue Secretome by 2-DE and NLC-MS/MS
Identification of Novel Biomarkers of Abdominal Aortic Aneurysms (AAA) by 2D-DIGE and MALDI-MS FROM AAA-Thrombus Conditioned Media
Metabolites Secreted by Human Atherothrombotic Aneurysm
Quantitative Analysis of Apolipoproteins in Human HDL by Top-Down Differential Mass Spectrometry
Quantitative Proteomics Analysis of High-Density Lipoproteins by Stable 18O-Isotope Labeling
Unraveling Biomarkers of Abdominal Aortic Aneurisms by iTRAQ Analysis of Depleted Plasma
Absolute Quantitation of Proteins in Human Blood by Multiplexed Multiple Reaction Monitoring Mass Spectrometry
Multiple Reaction Monitoring (MRM) of Plasma Proteins in Cardiovascular Proteomics
Proteomic Analysis of Plasma of Patients with Left Ventricular Remodeling After Myocardial Infarction. Usefulness of SELDI-TOF
Proteomic Analysis of Urinary Exosomes in Cardiovascular and Associated Kidney Diseases by Two Dimensional Electrophoresis and LC-MS/MS.