Bookedited by James L. Cole.
Summary: Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software. This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals.
Contents:
Next-generation AUC adds a spectral dimension: development of multiwavelength detectors for the analytical ultracentrifuge
Next-generation AUC: analysis of multiwavelength analytical ultracentrifugation data
Sedimentation veolocity: a classical perspective
Hydrodynamic modeling and its application in AUC
Calculations and publication-quality illustrations for analytifcal ultracentrifugation data - Sedimentation equilibrium analysis of ClpB self-association in diluted and crowded solutions
Analysis of linked equilibria
Elucidating complicated assembling systems in biology using size-and-shape analysis of sedimentation velocity data
Quaternary structure analyses of an essential oligomeric enzyme - Charterization of intrinsically disordered proteins by analytical ultracentrifugation
Sedimentation velocity analysis of the size distribution of amyloid oligomers and fibrils
AUC and small-angle scattering for membrane proteins
Hydrodynamic models of G-quadraplex structures
Analytical ultracentrifugation as a tool to study nonspecific protein-DNA interactions
Characterization of homogenous, cooperative protein-DNA clusters by sedimentation equilibrium analytical ultracentrifugation and atomic force microscopy
Sedimentation velocity anlaysis of large oligomeric chromatin complexes using interference detection
Dissecting steroid receptor function by analytical ultracentrifugation
Ultracentrifuge methods for the analysis of polysaccharides, glycoconjugates, and lignins
Analytical ultracentrifugation and its role in development and research of therapeutical proteins
Guidance to achieve accurate aggregate quantitation in biopharmaceuticals by SV-AUC
Protein assembly in serum and the differences from assembly in buffer.