Bookeditor, Phil Langton.
Summary: This concise, easy-to-follow text gives an insight into core techniques and practices in biomedical research and how, when and why a technique should be used and presented in the literature. Readers are alerted to common failures and misinterpretations that may evade peer review and are equipped with the judgment necessary to be properly critical of the findings claimed by research articles. --from publisher description
Contents:
Section A: Basic Principles. Philosophy of Science / James Ladyman
Ingredients of Experimental Design / Nick Colegrave
Statistics: A Journey that Needs a Guide / Gordon Drummond
Section B: Cell and Molecular. Organ Bath Pharmacology / Emma Robinson
Small Vessel Myography / Tomoko Kamishima, John M Quayle
Mammalian Cell Cultures: The Example of Airway Epithelial Cell Cultures for Cystic Fibrosis Research / Scott H Randell
Electron Microscopy (TEM and SEM) / Paul Verkade
Fluorescence Microscopy / Mark Jepson
Intracellular 'Sharp' Microelectrode Recording / Helena C Parkington, Harold A Coleman
Single Electrode Voltage-Clamp (SEVC) / Harold A Coleman, Helena C Parkington
Patch Clamp Recording / Neil Bannister, Phil Langton
Production of Antibodies / Elek Molnar
Immunocytochemistry and Immunohistochemistry / Elek Molnar
Immunoprecipitation (IP) / David Bates
Immunoblotting (Western) / Samantha F Moore, Joshua S Savage, Ingeborg Hers
Applications of Green Fluorescent Protein (GFP) / Mark Jepson
Fluorescent Measurement of Ion Activity in Cells / Helen Kennedy
Detection of Exocytosis : Real Time / Anja Teschemacher
Viral Vector Transgenesis / Anja Teschemacher
Polymerase Chain Reaction (PCR) and Reverse Transcription (RT)-PCR / Lucy F Donaldson
Hybridisation (ISH) / Lucy F Donaldson
Methods of Interference (Antisense, siRNAs and Dominant Negative Mutations) / Allison Fulford
Transcriptome Analysis: Microarrays / Charles Hindmarch
Experimental Proteomics / Thierry Le Bihan
Section C: . Behavioural Methodology / Emma Robinson
Genetically Modified Mouse Models / Nina Balthasar
Wireless Recording of Cardiovascular Signals / Julian FR Paton, Fiona D McBryde
Electrical Stimulation Methods / Jon Wakerley
Extracellular Recording / Jon Wakerley
Antidromic Identification / Jon Wakerley
Event-Triggered Averaging, Including Spike-Triggered Averaging / Richard Apps
Axonal Transport Tracing of CNS Pathways / John Crabtree
Cardiovascular Methods: General Considerations for Human Studies / Erica A Wehrwein, Michael J Joyner
Measuring Cardiac Output in Humans / Erica A Wehrwein, Michael J Joyner
Measuring Peripheral Blood Flow in Humans / Erica A Wehrwein, Michael J Joyner.