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  • Book
    Barbara Janson Cohen, Ann DePetris.
    Contents:
    Concepts of medical terminology
    Suffixes
    Prefixes
    Cells, tissues, and organs
    Body structure
    Disease
    Diagnosis and treatment. Surgery
    Drugs
    Circulation : the cardiovascular and lymphatic systems
    Blood and immunity
    The respiratory system
    The digestive system
    The urinary system
    The male reproductive system
    The female reproductive system. Pregnancy and birth
    The endocrine system
    The nervous system and behavioral disorders
    The senses
    The skeleton
    The muscular system
    The skin.
    Print Access Request
    Location
    Version
    Call Number
    Items
    Books: General Collection (Downstairs)
    R123 .C56 2013
    1
  • Article
    Ljungsted I, Ekman B, Sjöholm I.
    Biochem J. 1978 Jan 15;170(1):161-5.
    Horse anti-(human lymphocyte) globulin was immobilized together with fluorescein labelled dextran in spherical microparticles of polyacrylamide (AHLG-particles). The particles had a diameter of 1-5 micrometer and a density of 1.12g/cm3, with globulin exposed on the surface. Human lymphocytes bearing the antigen (thymus-derived lymphocytes) bound the particles, which were easily detected by fluorescence microscopy. In this way, about 58% of circulating human lymphocytes were able to bind AHLG-particles at 23 degrees C. Non-specific binding was low, only 3% when human serum albumin was present in the buffer, and only 4% when non-specific horse globulins were incorporated in the microparticles. The cell-particle complexes could be separated from cells that had not reacted by density-gradient centrifugation in Ficoll/metrizoate. The viability was not changed after the separation procedure. The number of cells binding AHLG-particles corresponded well the the relative amount of T-cells. When the cells binding AHLG-particles were separated from the lymphocytes, the number of T-cells decreased remarkably, indicating that the antibodies bind preferably to the T-cell population. Concanavalin A immobilized in microparticles was sufficiently exposed to initiate the agglutination of the lymphocytes. The agglutination was completely inhibited by preincubating the microparticles with alpha-methyl mannoside.
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