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- BookBarbara Janson Cohen, Ann DePetris.Contents:
Concepts of medical terminology
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Cells, tissues, and organs
Body structure
Disease
Diagnosis and treatment. Surgery
Drugs
Circulation : the cardiovascular and lymphatic systems
Blood and immunity
The respiratory system
The digestive system
The urinary system
The male reproductive system
The female reproductive system. Pregnancy and birth
The endocrine system
The nervous system and behavioral disorders
The senses
The skeleton
The muscular system
The skin. - ArticleLjungsted I, Ekman B, Sjöholm I.Biochem J. 1978 Jan 15;170(1):161-5.Horse anti-(human lymphocyte) globulin was immobilized together with fluorescein labelled dextran in spherical microparticles of polyacrylamide (AHLG-particles). The particles had a diameter of 1-5 micrometer and a density of 1.12g/cm3, with globulin exposed on the surface. Human lymphocytes bearing the antigen (thymus-derived lymphocytes) bound the particles, which were easily detected by fluorescence microscopy. In this way, about 58% of circulating human lymphocytes were able to bind AHLG-particles at 23 degrees C. Non-specific binding was low, only 3% when human serum albumin was present in the buffer, and only 4% when non-specific horse globulins were incorporated in the microparticles. The cell-particle complexes could be separated from cells that had not reacted by density-gradient centrifugation in Ficoll/metrizoate. The viability was not changed after the separation procedure. The number of cells binding AHLG-particles corresponded well the the relative amount of T-cells. When the cells binding AHLG-particles were separated from the lymphocytes, the number of T-cells decreased remarkably, indicating that the antibodies bind preferably to the T-cell population. Concanavalin A immobilized in microparticles was sufficiently exposed to initiate the agglutination of the lymphocytes. The agglutination was completely inhibited by preincubating the microparticles with alpha-methyl mannoside.