Bookedited by Harry J. Gilbert.
Summary: Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume in the Methods in Enzymology series comprehensively covers this topic.
Contents:
Measurement of endo-1,4-[beta]-Glucanase
Biomass Conversion Determined via Fluorescent Cellulose Decay Assay
The Analysis of Saccharification in Biomass Using an Automated High-Throughput Method
Studies of Enzymatic Cleavage of Cellulose Using Polysaccharide Analysis by Carbohydrate
Measuring Processivity
Distinguishing Xyloglucanase Activity in endo-[beta](1-->4)glucanases
Methods for Structural Characterization of the Products of Cellulose- and Xyloglucan-Hydrolyzing Enzymes
The Crystallization and Structural Analysis of Cellulases (and Other Glycoside Hydrolases)
Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose
Small-Angle X-ray Scattering and Crystallography
Quantitative Approaches to The Analysis of Carbohydrate-Binding Module Function
In Situ Detection of Cellulose with Carbohydrate-Binding Modules
Interactions Between Family 3 Carbohydrate Binding Modules (CBMs) and Cellulosomal Linker Peptides
Approaches for Improving Thermostability Characteristics in Cellulases
Thermophilic Glycosynthases for Oligosaccharides Synthesis
Engineering Cellulase Activity into Clostridium acetobutylicum
Transformation of Clostridium Thermocellum by Electroporation
Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus
Methods for the Isolation of Cellulose-Degrading Microorganisms
Metagenomic Approaches to the Discovery of Cellulases
Escherichia coli Expression, Purification, Crystallization, and Structure Determination of Bacterial
Measurements of Relative Binding of Cohesin and Dockerin Mutants Using an Advanced ELISA Technique
Designer Cellulosomes for Enhanced Hydrolysis of Cellulosic Substrates
High-Throughput Screening of Cohesin Mutant Libraries on Cellulose Microarrays.