BookJohn M. Butler.
Summary: Intended as a companion to the Fundamentals of Forensic DNA Typing volume published in 2009, Advanced Topics in Forensic DNA Typing: Methodology contains 18 chapters with 4 appendices providing up-to-date coverage of essential topics in this important field and citation to more than 2800 articles and internet resources. The book builds upon the previous two editions of John Butler's internationally acclaimed Forensic DNA Typing textbook with forensic DNA analysts as its primary audience. This book provides the most detailed information written to-date on DNA databases, low-level DNA, validation, and numerous other topics including a new chapter on legal aspects of DNA testing to prepare scientists for expert witness testimony. Over half of the content is new compared to previous editions. A forthcoming companion volume will cover interpretation issues. Contains the latest information - hot-topics and new technologies Well edited, attractively laid out, and makes productive use of its four-color format Author John Butler is ranked as the number one "high-impact author in legal medicine and forensic science, 2001 to 2011" by ScienceWatch.com.
Contents:
Sample collection, storage, and characterization
DNA extraction methods
DNA quantitation
PCR amplification: capabilities and cautions
Short tandem repeat (STR) loci and kits
Capillary electrophoresis: principles and instrumentation
Quality assurance and validation
DNA databases: uses and issues
Missing persons and disaster victim identification efforts
Degraded DNA
Low-level DNA testing: issues, concerns, and solutions
Single nucleotide polymorphisms and applications
Y-chromosome DNA testing
Mitochondrial DNA analysis
X-chromosome analysis
Non-human DNA
New technologies and automation
Legal aspects of DNA testing and the scientific expert in court.