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  • Book
    Yves-Gérard Illouz, Aris Sterodimas, editors.
    Contents:
    Cellular and Molecular Aspects of Adipose Tissue
    Isolation of Stem Cells from Human Adipose Tissue: Technique, Problems, and Pearls
    Principles of Liposuction
    Clinical Applications of Liposuction
    Principles of Autologous Fat Transplantation
    Clinical Applications of Autologous Fat Transplantation
    Adipose Stem Cells: From Liposuction to Adipose Tissue Engineering
    Storage of Adipose Stem Cells
    Engineered Scaffolds and Matrices: Tailored Biomaterials for Adipose Stem Cell Engineering
    Fundamentals and Principles of Biomolecules in Adipose Stem Cell Engineering
    Gene Therapy Used for Adipose Stem Cell Engineering
    Stem Cells from Human Adipose Tissue: A New Tool for Pharmacological Studies and for Clinical Applications
    Role of Adipose Stem Cells Therapy in Obesity
    Stem Cell Based Cardioregeneration and Adipose Tissue
    Adipose Stem Cell Engineering: Characterization and Current Application in Neurological Tissue Repair
    Adipose Stem Cell Engineering: Clinical Applications in Plastic and Reconstructive Surgery
    Orthopedic Use of Adipose-Derived Stem Cells
    Adipose-Derived Stem Cells: Characterization and Application in Urology
    Adipose Stem Cell Engineering: Characterization and Current Application in Otolaryngology
    Adipose Stem Cell Technologies for Tissue Regeneration in Dentistry
    Adipose Stem Cells, Tissue Engineering, and Solid Organ Transplantation and Regeneration
    Industrial Approaches to Adipose Stem Cells Engineering
    Future Research in Adipose Stem Cell Engineering
    Conclusions and Future Directions.
    Digital Access Springer 2011
  • Article
    Anderson P, Smith DH.
    Infect Immun. 1977 Feb;15(2):472-7.
    The capsular polysaccharide (CP) of Haemophilus influenzae type b is known to be spontaneously released from the cells in culture. The CP is precipitable from culture supernatant by the cationic detergent hexadecyltrimethylammonium. Most of the nucleic acid and some of the protein, but almost none of the endotoxin, in the supernatant are co-precipitated. Extraction of the precipitate with progressively stronger NaCl solutions separates nucleic acid and protein from the CP and also effects a molecular size fractionation. Residual endotoxin and protein can be reduced by extraction with cold phenol and ultracentrifugation. The resulting preparation has ribose, ribitol, and phosphate as principal components and contains less than 1% other sugars, protein, or nucleic acid; it elutes on Sepharose 2B as a symmetrical peak with Kav 0.51.
    Digital Access Access Options