Bookedited by Samir S. Ayoub, Roderick J. Flower, Michael P. Seed.
Contents:
Part I. In vitro protocols for studying expression and activity of cyclooxygenase enzymes and characterisation of their inhibition patterns
1. In vitro cyclo-oxygenase expression and activity protocols : introduction to Part I / Roderick J. Flower
2. Techniques used to study regulation of cyclooxygenase-2 promoter sites / Hiroyasu Inoue and Rieko Nakata
3. Purification of recombinant human COX-1 and COX-2 / James K. Gierse
4. Cloning and expression of cyclooxygenase-1 and cyclooxygenase-2 / Nicholas R. Staten and Beverly A. Reitz
5. Expression of cyclooxygenase isoforms in the baculovirus expression system / John C. Hunter, Natalie M. Myres, and Daniel L. Simmons
6. Peroxidase active site activity assay / Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett
7. Study of inhibitors of the PGH synthases for which potency Is regulated by the redox state of the enzymes / Olivier Boutaud and John A. Oates
8. Different methods for testing potential cyclooxygenase-1 and cyclooxygenase-2 inhibitors / Stefan Laufer and Sabine Luik
9. In vitro cyclooxygenase activity assay in tissue homogenates / Samir S. Ayoub
10. Techniques used to characterize the binding of cyclooxygenase Inhibitors to the cyclooxygenase active site / William F. Hood
11. In vitro Assays for cyclooxygenase activity and inhibitor characterization / Mark C. Walker and James K. Gierse
-- Part II. Extraction and measurement of prostanoids and isoprostanes
12. Extraction and measurement of prostanoids and isoprostanes : introduction to Part II / Paola Patrignani
13. Prostanoid extraction and measurement / Lorenzo Polenzani and Samir S. Ayoub
14. Measurement of 8-iso-prostaglandin F2a in biological fluids as a measure of lipid peroxidation / Stefania Tacconelli, Marta L. Capone, and Paola Patrignani
-- Part III. In vivo models to study involvement of cyclooxygenase products in health and disease
15. In vivo models to study cyclooxygenase products in health and disease: introduction to Part III / Derek W. Gilroy, Melanie Stables, and Justine Newson
16. Protocols to assess the gastrointestinal side effects resulting from inhibition of Cyclo-Oxygenase isoforms / Brendan J.R. Whittle
17. Cyclooxygenase enzymes and their products in the carrageenan-induced pleurisy in rats / Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed
18. Iloprost-induced nociception : determination of the site of anti-nociceptive action of cyclooxygenase inhibitors and the Involvement of cyclooxygenase products in central mechanisms of nociception / Samir S. Ayoub and Regina M. Botting.