Bookedited by Marten H. Hofker, Jan van Deursen.
Contents:
Introduction: strategies for developing genetically modified mice / Marten H. Hofker
Genetic modification of the mouse: general technology - pronuclear and blastocyst injection / Jan Willem Voncken
Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos / Jérôme Artus and Anna-Katerina Hadjantonakis
Cryopreservation of mouse spermatozoa and in vitro fertilization / Naomi Nakagata
Autopsy and histological analysis of the transgenic mouse / Marion J.J. Gijbels and Menno P.J. de Winther
Transgene design / Bart van de Sluis and Jan Willem Voncken
Inducible transgenic mouse models / Thomas L. Saunders
Lentiviral transgenesis / Terunaga Nakagawa and Casper C. Hoogenraad
Transgenesis in mouse embryonic stem cells / Janine van Ree, Wei Zhou, Ming Li, and Jan M. van Deursen
Engineering BAC reporter gene constructs for mouse transgenesis / Yu Fu and Peter Maye
Targeting vector construction through recombineering / Liviu A. Malureanu
Generating conditional knockout mice / Roland H. Friedel, Wolfgang Wurst, Benedikt Wefers, and Ralf Kühm
Hypomorphic mice / Darren J. Baker
MICER targeting vectors for manipulating the mouse genome / Chunbong Liu, Paul F. Szurek, and Y. Eugene Yu
Knock-in approaches / Anton J.M. Roebroek, Philip L.S.M. Gordts, and Sara Reekmans
Generation of a series of knock-in alleles using RMCE in ES cells / Anton J.M. Roebroek, Philip L.S.M. Gordts, and Sara Reekmans
Selection of targeting mutants from a library of randomly mutagenized ES cells / Kyoji Horie, George Gaitanaris, and Alexander Gragerov
Generation of genetically modified rodents using random ENU mutagenesis / Ruben van Boxtel and Edwin Cuppen
Bone marrow transplantations of study gene function in hematopoietic cells / Menno P.J. de Winther and Peter Heeringa
Adenovirus-mediated gene transfer / Ko Willems van Dijk, Kyriakos E. Kyreos, Frits J. Fallaux, and Jurre Hageman.