Bookedited by Dermot Walls and Sinéad T. Loughran.
Contents:
A digest of protein purification
Gel-filtration chromatography
Immunoaffinity chromatography
Avoiding proteolysis during protein chromatography
Scale-up of protein purification : Downstream processing issues
Phage display : A powerful technology for the generation of high specificity affinity reagents from alternative immune sources
Engineering protein stability
Microfluidics in protein chromatography
Tagging recombinant proteins to enhance solubility and aid purification
Storage and lyophilisation of pure proteins
Differential precipitation and solubilization of proteins
Ion-exchange chromatography : Basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase
Protein quantitation and analysis of purity
Purification of proteins fused to glutathione S-transferase
Purification of proteins fused to maltose-binding protein
Purification of proteins from baculovirus-infected insect cells
Purification of poly-histidine-tagged proteins
Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by cid/etd tandem mass spectrometry
Tag removal by site-specific cleavage of recombinant fusion proteins
Purification of antibodies using affinity chromatography
Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens
Measuring protein-protein interactions using biacore
Ultra-performance liquid chromatography-mass spectrometry of proteins
Hydrophobic interaction chromatography
Fast protein liquid chromatography
Shotgun proteomics : A qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS
Shotgun proteomics : A relative quantitative approach using off-gel electrophoresis and LC-MS/MS
Clinical proteomics : Liquid chromatography-mass spectrometry purification systems
Strategies for the purification of membrane proteins
A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases.