Bookedited by Ralf Kühn, Wolfgang Wurst.
Contents:
Overview on mouse mutagenesis / Ralf Kühn and Wolfgang Wurst
Construction of gene-targeting vectors by recombineering / Song-Choon Lee, Wei Wang, and Pentao Liu
Gene-trap vectors and mutagenesis / Silke De-Zolt ... [et al.]
Chromosome engineering in ES cells / Louise vander Weyden, Charles Shaw-Smith, and Allan Bradley
Gene modification in embryonic stem cells by single-stranded DNA oligonucleotides / Marieke Aarts ... [et al.]
Generation of shRNA transgenic mice / Christiane Hitz ... [et al.]
Mutagenesis of mouse embryonic stem cells with ethylmethanesulfonate / Robert Munroe and John Schimenti
Gene targeting in mouse embryonic stem cells / Lino Tessarollo ... [et al.]
Manipulating mouse embryonic stem cells / Eileen Southon and Lino Tessarollo
ES cell line establishment / Heidrun Kern and Branko Zevnik
Generation of double-knockout embryonic stem cells / Eva Wielders, Marleen Dekker, and Hein te Riele
Differentiation analysis of pluripotent mouse embryonic stem (ES) cells in vitro / Insa S. Schroeder ... [et al.]
Cloning of ES cells and mice by nuclear transfer / Sayaka Wakayama, Satoshi Kishigami, and Teruhiko Wakayama
Isolation, microinjection, and transfer of mouse blastocysts / Susan W. Reid and Lino Tessarollo
Aggregation chimeras : combining ES cells, diploid, and tetraploid embryos / Mika Tanaka ... [et al.]
VelociMouse : fully ES cell-derived F0-generation mice obtained from the injection of ES cells into eight-cell-stage embryos / Thomas M. DeChiara ... [et al.]
Generation of Cre recombinase-expressing transgenic mice using bacterial artificial chromosomes / Jan Rodriguez Parkitna, David Engblom, and Günther Schütz
Inducible Cre mice / Susanne Feil, Nadejda Valtcheva, and Robert Feil
Creation and use of a Cre recombinase transgenic database / Andras Nagy, Lynn Mar, and Graham Watts
Transposon mutagenesis in mice / David A. Largaespada
Lentiviral transgenesis / Alexander Pfeifer and Andreas Hofmann
Sperm cryopreservation and in vitro fertilization / Susan Marschall, Auke Boersma, and Martin Hrabé de Angelis
Influence of genetic background on genetically engineered mouse phenotypes / Thomas Doetschman
Pathologic phenotyping of mutant mice / Roderick T. Bronson
Systemic first-line phenotyping / Valérie Gailus-Durner ... [et al.]