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  • Book
    edited by Massimo Sabatini, Philippe Pastoureau, Frédéric De Ceuninck.
    Digital : Springerv. 1
    Digital : Springerv. 2
    A simple and reliable assay of proteoglycan synthesis by cultured chondrocytes -- Assays of proteoglycan and collagen degradation in cultures of rabbit cartilage explants -- Production of antibodies against degradative neoepitopes in aggrecan -- Immunoassays for collagens in chondrocyte and cartilage explant cultures -- Detection of apoptosis in cartilage in situ and in isolated chondrocytes -- Expression, activity, and regulation of MAP kinases in cultured chondrocytes -- Mechanical loading of chondrocytes embedded in 3D constructs: in vitro methods for assessment of morphological and metabolic response to compressive strain -- In vitro physical stimulation of tissue-engineered and native cartilage -- v. 1. Cellular and molecular tools. Culture and phenotyping of chondrocytes in primary culture -- Culture of chondrocytes in alginate beads -- Immortalization of human articular chondrocytes for generation of stable, differentiated cell lines -- Culture of immortalized chondrocytes and their use as models of chondrocyte function -- Generation of pluripotent stem cells and their differentiation to the chondrocytic phenotype -- Semiquantitative analysis of gene expression in cultured chondrocytes by RT-PCR -- Quantification of mRNA expression levels in articular chondrocytes with PCR technologies -- RNA extraction from cartilage -- Gene expression analysis in cartilage by in situ hybridization -- Analysis of differential gene expression in healthy and osteoarthritic cartilage and isolated chondrocytes by microarray analysis -- High-efficiency nonviral transfection of primary chondrocytes -- In vitro gene transfer to chondrocytes and synovial fibroblasts by adenoviral vectors -- Changes of chondrocyte metabolism in vitro: an approach by proteomic analysis -- Analysis of chondrocyte functional markers and pericellular matrix components by flow cytometry -- v. 2. Structure and in vivo analysis. Generation and use of transgenic mice as models of osteoarthritis -- Development and clinical application in arthritis of a new immunoassay for serum type IIA procollagen NH2 propeptide -- Histological and immunohistological studies on cartilage -- Histochemical visualization of the cartilage hyaladherins using a biotinylated hyaluronan oligosaccharide bioaffinity probe -- Methods for cartilage and subchondral bone histomorphometry -- Laser-mediated microdissection as a tool for molecular analysis in arthritis -- Analysis of protein distribution in cartilage using immunofluorescence and laser confocal scanning microscopy -- Molecular and biochemical assays of cartilage components -- Mechanical characterization of native and tissue-engineered cartilage -- Noninvasive study of human cartilage structure by MRI -- High-resolution MRI techniques for studies in small-animal models -- High-resolution imaging of osteoarthritis using microcomputed tomography -- High-resolution ultrasonograpy for analysis of age- and disease-related cartilage changes -- Evaluation of cartilage composition and degradation by high-resolution magic-angle spinning nuclear magnetic resonance -- Pulsed-field gradient-nuclear magnetic resonance (PFG NMR) to measure the diffusion of ions and polymers in cartilage: applications in joint diseases -- Dynamics of collagen in articular cartilage studied by solid-state NMR methods -- Computerized protocol for anatomical and functional studies of joints.