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- Bookedited by Massimo Sabatini, Philippe Pastoureau, Frédéric De Ceuninck.Contents:
A simple and reliable assay of proteoglycan synthesis by cultured chondrocytes
Assays of proteoglycan and collagen degradation in cultures of rabbit cartilage explants
Production of antibodies against degradative neoepitopes in aggrecan
Immunoassays for collagens in chondrocyte and cartilage explant cultures
Detection of apoptosis in cartilage in situ and in isolated chondrocytes
Expression, activity, and regulation of MAP kinases in cultured chondrocytes
Mechanical loading of chondrocytes embedded in 3D constructs: in vitro methods for assessment of morphological and metabolic response to compressive strain
In vitro physical stimulation of tissue-engineered and native cartilage
v. 1. Cellular and molecular tools. Culture and phenotyping of chondrocytes in primary culture
Culture of chondrocytes in alginate beads
Immortalization of human articular chondrocytes for generation of stable, differentiated cell lines
Culture of immortalized chondrocytes and their use as models of chondrocyte function
Generation of pluripotent stem cells and their differentiation to the chondrocytic phenotype
Semiquantitative analysis of gene expression in cultured chondrocytes by RT-PCR
Quantification of mRNA expression levels in articular chondrocytes with PCR technologies
RNA extraction from cartilage
Gene expression analysis in cartilage by in situ hybridization
Analysis of differential gene expression in healthy and osteoarthritic cartilage and isolated chondrocytes by microarray analysis
High-efficiency nonviral transfection of primary chondrocytes
In vitro gene transfer to chondrocytes and synovial fibroblasts by adenoviral vectors
Changes of chondrocyte metabolism in vitro: an approach by proteomic analysis
Analysis of chondrocyte functional markers and pericellular matrix components by flow cytometry
v. 2. Structure and in vivo analysis. Generation and use of transgenic mice as models of osteoarthritis
Development and clinical application in arthritis of a new immunoassay for serum type IIA procollagen NH2 propeptide
Histological and immunohistological studies on cartilage
Histochemical visualization of the cartilage hyaladherins using a biotinylated hyaluronan oligosaccharide bioaffinity probe
Methods for cartilage and subchondral bone histomorphometry
Laser-mediated microdissection as a tool for molecular analysis in arthritis
Analysis of protein distribution in cartilage using immunofluorescence and laser confocal scanning microscopy
Molecular and biochemical assays of cartilage components
Mechanical characterization of native and tissue-engineered cartilage
Noninvasive study of human cartilage structure by MRI
High-resolution MRI techniques for studies in small-animal models
High-resolution imaging of osteoarthritis using microcomputed tomography
High-resolution ultrasonograpy for analysis of age- and disease-related cartilage changes
Evaluation of cartilage composition and degradation by high-resolution magic-angle spinning nuclear magnetic resonance
Pulsed-field gradient-nuclear magnetic resonance (PFG NMR) to measure the diffusion of ions and polymers in cartilage: applications in joint diseases
Dynamics of collagen in articular cartilage studied by solid-state NMR methods
Computerized protocol for anatomical and functional studies of joints.