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  • Book
    edited by Mark Waugh.
    Digital : Springer2016
    Method for assaying the lipid kinase phosphatidylinositol-5-phosphate 4-kinase α in quantitative high-throughput screening (qHTS) bioluminescent format / Mindy I. Davis, Atsuo T. Sasaki, and Anton Simeonov -- Assaying ceramide synthase activity in vitro and in living cells using liquid chromatography-mass spectrometry / Xin Ying Lim, Russell Pickford, and Anthony S. Don -- Fluorescent assays for ceramide synthase activity / Timothy A. Couttas and Anthony S. Don -- Identification of the interactome of a palmitoylated membrane protein, phosphatidylinositol 4-kinase type II alpha / Avanti Gokhale ... [et al.] -- Measurement of long-chain fatty Acyl-CoA synthetase activity / Joachim Füllekrug and Margarete Poppelreuther -- Qualitative and quantitative in vitro analysis of phosphatidylinositol phosphatase substrate specificity / Laura Ren Huey Ip and Christina Anja Gewinner -- Luciferase reporter assays to assess liver X receptor transcriptional activity / Matthew C. Gage, Benoit Pourcet, and Inés Pineda-Torra -- Metabolically biotinylated reporters for electron microscopic imaging of cytoplasmic membrane microdomains / Kimberly J. Krager and John G. Koland -- Fluorescence recovery after photobleaching analysis of the diffusional mobility of plasma membrane proteins : HER3 mobility in breast cancer cell membranes / Mitul Sarkar and John G. Koland -- Isolation and analysis of detergent-resistant membrane fractions / Massimo Aureli ... [et al.] -- Detection of isolated mitochondria-associated ER membranes using the sigma-1 receptor / Abasha Lewis, Shang-Yi Tsai, and Tsung-Ping Su -- Using surface plasmon resonance to quantitatively assess lipid-protein interactions / Kathryn Del Vecchio and Robert V. Stahelin -- Analyzing protein-phosphoinositide interactions with liposome flotation assays / Ricarda A. Busse ... [et al.] -- High-throughput fluorometric assay for membrane-protein interaction / Wonhwa Cho, Hyunjin Kim, and Yusi Hu -- Guidelines for the use of protein domains in acidic phospholipid imaging / Matthieu Pierre Platre and Yvon Jaillais -- Analysis of sphingolipid synthesis and transport by metabolic labeling of cultured cells with [3H]serine / Neale D. Ridgway -- Determination and characterization of tetraspanin-associated phosphoinositide-4 kinases in primary and neoplastic liver cells / Krista Rombouts and Vinicio Carloni -- Analysis of the phosphoinositide composition of subcellular membrane fractions / Deborah A. Sarkes and Lucia E. Rameh -- Single-molecule imaging of signal transduction via GPI- anchored receptors / Kenichi G. N. Suzuki -- Measuring phosphatidylinositol generation on biological membranes / Mark Waugh -- Assay for CDP-diacylglycerol generation by CDS in membrane fractions / Mark Waugh.