Bookedited by Tamas Dalmay.
Contents:
In situ detection of microRNAs in animals / Dylan Sweetman
Detection of microRNAs in plants by in situ hybridisation / Eva Varallyay and Zoltan Havelda
Detecting sRNAs by northern blotting / Sara Lopez-Gomollon
Profiling microRNAs by real-time PCR / Nanna Jacobsen, Ditte Andreasen, and Peter Mouritzen
Detection of small RNAs and microRNAs using deep sequencing technology / Ericka R. Havecker
Detection of microRNAs in prostate cancer cells by mircoRNA array / Xiaoqing Tang [and others]
MicroRNA knock down by cholesterol-conjugated antisence oligos in mouse organ culture / Sharon Kredo-Russo and Eran Hornstein
Protocols for use of homologous recombination gene targeting to produce microRNA mutants in drosophila / Ya-Wen Chen, Ruifen Weng, and Stephen M. Cohen
Engineering elements for gene silencing : the artificial microRNAs technology / Pablo Andres Manavella and Ignacio Rubio-Somoza
Mimicry technology : suppressing small RNA activity in plants / Ignacio Rubio-Somoza and Pablo Andres Manavella
Experimental validation of microRNA targets using a luciferase reporter system / Francisco E. Nicolas
Experimental identification of microRNA targets by immunoprecipitation of argonaute protein complexes / Michaela Beitzinger and Gunter Meister
Comprehensive identification of miRNA target sites in live animals / Dimitrios G. Zisoulis, Gene W. Yeo, and Amy E. Pasquinelli
Target validation of plant microRNAs / Cesar Llave [and others]
High-throughput sequencing-based methodology to identify all uncapped and cleaved RNA molecules in eukaryotic genomes / Matthew W. Endres, Rebecca T. Cook, and Brian D. Gregory.