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  • Book
    by Lynda J. Campbell.
    Summary: [Publisher-supplied data] Cytogenetic studies of malignancy have become an essential tool in the clinical management of cancer patients. Cancer Cytogenetics: Methods and Protocols presents eminently practical key cytogenetic and FISH techniques for every stage of diagnostic service. Experts in the field describe detailed cytogenetic analysis methods, fluorescence in situ hybridization and array methods currently being applied to investigate and diagnose different varieties of cancer. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory protocols. The authors of the various chapters have also provided extensive notes to guide individuals who are new to these methods through the pitfalls that bedevil all such testing. Authoritative and accessible, Cancer Cytogenetics: Methods and Protocols serves as an ideal guide to scientists of all backgrounds, allowing them to either establish new techniques in their laboratories or find the different variations of standard methods helpful in improving their results.

    Contents:
    Introduction / Lynda J. Cambell
    Evolution of cytogenetic methods in the study of cancer / Lynda J. Campbell
    Fluorescence in situ hybridization methods and troubleshooting applied to fixed cell suspensions / John Swansbury, Toon Min, and Shilani Aruliah
    Chronic myeloid leukemia : cytogenetic methods chronic myeloid leukemia : cytogenetic methods / Christine M. Morris
    Cytogenetic analysis in acute myeloid leukaemia / Lynda J. Campbell and Joanne S. White
    Cytogenetics in myelodysplastic syndromes / Kazuma Ohyashiki, Atsushi Kodama, and Junko H. Ohyashiki
    Cytogenetics of myeloproliferative neoplasms / Lynda J. Campbell
    Acute lymphoblastic leukaemia / Claire Schwab and Christine J. Harrison
    Cytogenetic methods in chronic lymphocytic leukemia / Claudia Haferlach and Ulrike Bacher
    Genetic abnormalities in non-hodgkin's lymphoma as revealed by conventional and molecular cytogenetics methods of analyses / Suresh C. Jhanwar and Ryan C. Denley
    Metaphase cytogenetic techniques in multiple myeloma / Jeffrey R. Sawyer
    Detection of chromosome abnormalities using cytoplasmic immunoglobulin staining and FISH in myeloma / Bruce R. Mercer and Kathleen C. Rayeroux
    Cytogenetics of solid tumours / Robyn Lukeis and Mary Suter
    Fluorescence in situ hybridization on formalin-fixed, paraffin-embedded tissue sections / Adrian Zordan
    Use of M-FISH and M-BAND to define chromosome abnormalities / Ruth N. MacKinnon and Ilse Chudoba
    Utility of array comparative genomic hybridization in cytogenetic analysis / Rashmi R. Singh, K-John J. Cheung, and Douglas E. Horsman
    Single nucleotide polymorphism microarray analysis of genetic alterations in cancer / Charles G. Mullighan
    Cytogenetic analysis and reporting / Lynda J. Campbell.
    Digital Access Springer 2011