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  • Book
    edited by Irina Artsimovitch, Thomas J. Santangelo.
    Contents:
    Mapping the Escherichia coli transcription elongation complex with exonuclease III / Zhaokun Liu and Irina Artsimovitch
    Purification of bacterial RNA polymerase : tools and protocols / Vladimir Svetlov and Irina Artsimovitch
    Monitoring translocation of multisubunit RNA polymerase along the DNA with fluorescent base analogues / Anssi M. Malinen, Matti Turtola, and Georgiy A. Belogurov
    In vitro and in vivo methodologies for studying the sigma-54-dependent transcription / Martin Buck [and nine others]
    Methods for the assembly and analysis of in vitro transcription-coupled-to-translation systems / Daniel Castro-Roa and Nikolay Zenkin
    Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and Straudinger-Bertozzi ligation / Anirban Chakraborty [and seven others]
    Reconstruction of factor-dependent, promotor proximal pausing in Drosophila nuclear extracts / Jian Li and David S. Gilmour
    Direct competition assay for transcription fidelity / Lucyna Lubkowska and Maria L. Kireeva
    Single-stranded DNA aptamers for functional probing of bacterial RNA polymerase / Danil Pupov and Andrey Kulbachinskiy
    Biochemical analysis of transcription termination by RNA polymerase III from yeast Saccharomyces cerevisiae / Aneeshkumar G. Arimbasseri and Richard J. Maraia
    Use of RNA polymerase molecular beacon assay to measure RNA polymerase interactions with model promotor fragments / Vladimir Mekler and Konstantin Severinov
    Preparation of cDNA libraries for high-throughput RNA sequencing analysis of RNA 5' ends / Irina O. Vvedenskaya, Seth R. Goldman, and Bryce E. Nickels
    In situ footprinting of E. coli transcription elongation complex with chloroacetaldehyde / A. Rachid Rahmouni and Christine Mosrin-Hauman
    Using solutes and kinetics to probe large conformational changes in the steps of transcription initiation / Emily F. Ruff, Wayne S. Kontur, and M. Thomas Record Jr.
    Manipulating archaeal systems to permit analyses of transcription elongation-termination decisions in vitro / Alexandra M. Gehring and Thomas J. Santangelo
    Purification of active RNA polymerase I from yeast / Francis Dean Appling and David Alan Schneider
    Transcription in archaea : preparation of Methanocaldococcus jannaschii transcription machinery / Katherine Smollet, Fabian Blombach, and Finn Werner
    Transcription in archaea ; in vitro transcription assays for mjRNAP / Katherine Smollet, Fabian Blombach, and Finn Werner
    Experimental analysis of hFACT action during pol II transcription in vitro / Fu-Kai Hsieh, Olga I. Kulaeva, and Vasily M. Studitsky
    ChIP-Seq for genome-scale analysis of bacterial DNA-binding proteins / Richard P. Bonocora and Joseph T. Wade.
    Digital Access Springer 2015