Bookedited by Volkmar Weissig, Marvin Edeas.
Summary: This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. "Mitochondrial Medicine: Volume II, Manipulating Mitochondrial Function" describes techniques developed for manipulating and assessing mitochondrial function under general pathological conditions and specific disease states. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, "Mitochondrial Medicine" provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.
Contents:
DQAsomes as the prototype of mitochondria-targeted pharmaceutical nanocarriers: Preparation, characterization, and use
Predicting mitochondrial targeting by small molecule xenobiotics within living cells using QSAR models
Targeting mitochondria with small molecules: the preparation of mitoB and mitoP as exomarkers of mitochondrial hydrogen peroxide
Synthesis of triphenylphosphonium phospholipid conjugates for the preparation of mitochondriotropic liposomes
Synthesis and evaluation of (1)(8)F-labeled fluoroalkyl triphenylphosphonium salts as mitochondrial voltage sensors in PET myocardial imaging
Bridging the gap between nature and antioxidant setbacks: Delivering caffeic acid to mitochondria
Mitochondriotropic nanoemulsified genistein-loaded vehicles for cancer therapy
Formulation and optimization of mitochondria-targeted polymeric nanoparticles
Ex vivo generation of functional immune cells by mitochondria-targeted photosensitization of cancer cells
Targeting the mitochondrial genome via a dual function MITO-porter: Evaluation of mtDNA levels and mitochondrial function
Mitochondrial, acidic, and cytosolic pHs determination by (3)(1)p NMR spectroscopy: Design of new sensitive targeted ph probes
Methodology for use of mitochondria-targeted cations in the field of oxidative stress-related research
Synthesis and testing of novel isomeric mitochondriotropic derivatives of resveratrol and quercetin
Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents
The effect of mitochondrially targeted anticancer agents on mitochondrial (super)complexes
Mitochondrial targeting of recombinant RNA
Mitochondrial targeting of catalytic RNAs
Allotopic expression of ATP6 in the mouse as a transgenic model of mitochondrial disease
Analysis of pollutant-induced changes in mitochondrial DNA methylation
ETS and DEAS studies of the reduction of xenobiotics in mitochondrial intermembrane space
Analysis of mitochondrial morphology and function under conditions of mitofusin 2 deficiency
Yeast as a tool to study mitochondrial retrograde pathway en route to cell stress response
Cell energy budget platform for assessment of cell metabolism
Induced pluripotent stem cells (iPSCs) for modeling mitochondrial DNA disorders
Cytoplasmic transfer methods for studying the segregation of mitochondrial DNA in mice
In vivo visualization and quantification of mitochondrial morphology in C. elegans
Mitochondrial DNA as a biosensor of UV exposure in human skin
Assessment of short- and medium-chain fatty acids on mitochondrial function in severe inflammation
Atomic force microscopy-based shape analysis of heart mitochondria
Enzymatic assays for probing mitochondrial apoptosis
A cybrid cell model for the assessment of the link between mitochondrial deficits and sporadic parkinson's disease
Analysis of mitochondrial network by imaging: Proof of technique in schizophrenia.