Bookedited by Roberto Biassoni, Laboratorio Medicina Molecolare, Dipartimento Medicina Traslazionale, Instituto Giannina Gaslini, Genova, Italy, Alessandro Raso, Laboratorio U.O.C. Neurochirugia, Instituto Giannina Gaslini, Genova, Italy.
Contents:
Twenty years of qPCR: A mature technology?
Minimum information necessary for quantitative real-time PCR experiments
Selection of reliable reference genes for RT-qPCR analysis
Introduction to digital PCR
mRNA and microRNA purity and integrity: The key to success in expression profiling
Mediator probe PCR: Detection of real-time PCR by label-free probes and a universal fluorogenic reporter
Absolute quantification of viral DNA: The quest for perfection
A multiplex real-time PCR-platform integrated into automated extraction method for the rapid detection and measurement of oncogenic HPV type-specific viral DNA load from cervical samples
Real-time PCR detection of mycoplasma pneumoniae in the diagnosis of community-acquired pneumonia
A sensible technique to detect mollicutes impurities in human cells cultured in GMP condition
Real-time quantification assay to monitor BCR-ABL1 transcripts in chronic myeloid leukemia
A reliable assay for rapidly defining transplacental metastasis using quantitative PCR
Circulating cell-free DNA in cancer
Gene expression analysis by qPCR in clinical kidney transplantation
Posttranscriptional regulatory networks: From expression profiling to integrative analysis of mRNA and microRNA data
Clinical applications using digital PCR
Developing noninvasive diagnosis for single-gene disorders: the role of digital PCR.