Bookedited by Jon Lorsch.
Contents:
Explanatory chapter: PCR primer design / Rubén Álvarez-Fernández
How plasmid preparation kits work / Laura Koontz
Introducing exogenous DNA into cells / Laura Koontz
Agarose gel electrophoresis / Laura Koontz
Analysis of DNA by southern blotting / Gary Glenn and Lefkothea-Vasiliki Andreou
Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) / Sara Lopez-Gomollon and Francisco Esteban Nicolas
Molecular cloning / Juliane C. Lessard
Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® recombination cloning technology and Flp-In T-REx® lines / Jessica Spitzer, Markus Landthaler, and Thomas Tuschl
Restrictionless cloning / Mikkel A. Algire
Isolation of plasmid DNA from bacteria / Lefkothea-Vasiliki Andreou
Preparation of genomic DNA from bacteria / Lefkothea-Vasiliki Andreou
Preparation of genomic DNA from Saccharomyces cerevisiae / Jessica S. Dymond
Isolation of genomic DNA from mammalian cells / Cheryl M. Koh
Sanger dideoxy sequencing of DNA / Sarah E. Walker and Jon Lorsch
Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform / Srinivasan Yegnasubramanian
Next generation sequencing / Srinivasan Yegnasubramanian
Generating mammalian stable cell lines by electroporation / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy
Transient mammalian cell transfection with polyethylenimine (PEI) / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy
Site-directed mutagenesis / Julia Bachman
PCR-based random mutagenesis / Jessica S. Dymond
Megaprimer method for mutagenesis of DNA / Craig W. Vander Kooi
Troubleshooting PCR / Kirstie Canene-Adams
Quantitative PCR / Jessica S. Dymond
General PCR / Kirstie Canene-Adams
Colony PCR / Megan Bergkessel and Christine Guthrie
Chemical transformation of yeast / Megan Bergkessel and Christine Guthrie
Transformation of E. coli via electroporation / Juliane C. Lessard
Transformation of chemically competent E. coli / Rachel Green and Elizabeth J. Rogers.