Book[edited by] P. Michael Conn.
Summary: Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields.
Contents:
Extraction/characterization of DNA / Leland J. Cseke, Joseph R. Herdy
Co-immunoprecipitation : isolation of protein signaling complexes from native tissues / Adele Stewart, Rory A. Fisher
Real-time PCR / Yibing Jia
Single-cell gel electrophoresis combined with lesion-specific enzymes to measure oxidative damage to DNA / Andrew R. Collins, Amaya Azqueta
High-throughout methods for the identification of protein purification conditions using a cleavable tag system / Mads Gabrielsen, Katherine S.H. Beckham, Andrew J. Roe
Two-dimensional difference gel electrophoresis (2D DIGE) / Jonathan S. Minden
Optimization of cell cycle measurement by time-lapse microscopy / Gabor Nagy, Gabor Kiraly, Gaspar Banfalvi
Transfection methods / A. Ruedel, A.K. Bosserhoff
Cell enrichment from human blood through red cell lysis / Brandon L. Miller, Jeffrey J. Chalmers, William G. Lowrie
Magnetic, batch separation / Jeffrey J. Chalmers, William G. Lowrie
Lateral flow sandwich assay utilizing upconverting phosophor (UCP) reporters / Elisa M. Tjon Kon Fat, William R. Abrams, R. Sam Niedbala, Paul L.A.A. Corstjens
In vivo modulation of microRNA levels during spinal cord regeneration / Juan Felipe Diaz Quiroz, Karen Echeverri
CometChip : single-cell microarray for high-throughput detection of DNA damage / Jing Ge, David K. Wood, David M. Weingeist, Sangeeta N. Bhatia, Bevin P. Engelward
Purification of cardiac cells / Fumiyuki Hattori, Keichi Fukuda
Tube formation in vitro angiogenesis assay / Diego Guidolin, Giovanna Albertin
Processing of transmission electron microscopy images for single-particle analysis of macromolecular complexes / Zaelle Devaux, Carlos Oscar S. Sorzano, Slavica Jonic
Asymmetric 3D in vitro assay for the study of tumor cell invasion / Vera Brekhman, Gera Neufeld
Isolation of myoblasts from muscle tissue by preplating / Yoon Ghil Park, Ahmi Baek, Sunmi Kim
High-content small molecule screening strategies for stem-cell-derived motor neurons / Eunju Pack-Chung, Courtney A. Ackeifi, Lida Katsimpardi, Kevin J. Kim, Maureen Sherry-Lynes, Nina R. Makhortova, Lee L. Rubin
Simple technique for quantifying apoptosis in 96-well plates / Shilo M. Smith, Deborah Ribble, Nathaniel B. Goldstein, David A. Norris, Yiqun G. Shellman
Cell screening using resistive-pulse sensing / Karthik Balakrishnan, Lydia L. Sohn
Quantitative analysis of basic motile behavior in amoeboid cells / Deborah Wessels, Spencer Kuhl, David R. Soll
Single cell deposition / Jun Liu, Christopher Moraes, Zhe Lu, Craig Simmons, Yu Sun
Enzyme sensors for living cells / Ning Chen, Shen Tang, Shenghui Xue, Jenny J. Yang
Myoblast purifying by magnetic-activated cell sorting (MACS) / Yoon Ghil Park, Ahmi Baek, Sunmi Kim.