Bookedited by Iain J. McEwan.
Contents:
Nuclear receptors : one big family / Iain J. McEwan
Methods for measuring ligand dissociation and nuclear receptor turnover in whole cells / Elizabeth M. Wilson
Flow cytometry as a tool for measurement of steroid hormone receptor protein expression in leukocytes / Cherie L. Butts and Esther M. Sternberg
X-ray crystallography of agonis/antagonist-bound receptors / Ashley C.W. Pike
FRAP and FRET methods to study nuclear receptors in living cells / Martin E. van Royen ... [et al.]
Receptor-DNA interactions : EMSA and footprinting / Jason T. Read ... [et al.]
Chromatin immunoprecipitation (ChIP) methodology and readouts / Charles E. Massie and Ian G. Mills
Yeast-based reporter assays for the functional characterization of cochaperone interactions with steroid hormone receptors / Heather A. Balsiger and Marc B. Cox
High throughput analysis of nuclear receptor-cofactor interactions / Michael L. Goodson, Behnom Farboud, and Martin L. Privalsky
Binding affinity and kinetic analysis of nuclear receptor/co-regulator interactions using surface plasmon resonance / Derek N. Lavery
Using RNA interference to study protein function / Carol D. Curtis and Ann M. Nardulli
Using intrinsic fluorescence emission spectroscopy to study steroid receptor and coactivator protein conformation dynamics / Kate Watt and Iain J. McEwan
Development of phosphorylation site-specific antibodies to nuclear receptors / Inés Pineda Torra ... [et al.]
Tissue-selective knockouts of steroid receptors : a novel paradigm in the study of steroid action / Karel De Gendt and Guido Verhoeven
Methods for identifying and studying genetic alterations in hormone-dependent cancers / Outi R. Saramäki, Kati K. Waltering, and Tapio Visakorpi.