Lane Medical Library powered by LaneConnex Technology

Essential Resources for Quantitative PCR

 

What is it?

Quantitative polymerase chain reaction (QPCR) is a set of powerful PCR techniques designed to quantify RNA transcripts. However, much up-front work is required for experiments to produce reliable data.

Specific considerations for QPCR include:
  • determining whether your transcript has alternatively spliced forms
  • primer and probe design, particularly to ensure specificity
  • sample preparation
  • normalization controls
  • detecting RNA degradation
  • preventing degradation
The resources below have been carefully selected to provide the fastest way to get going in the right way and to facilitate the process overall.

Key references

Source

Lane Librarian

Record created 1/9/2007.

 

Need Help?

  • Today's hours: 8 am – 10 pm
  • Hours
School of Medicine