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Molecular Biology

  • 2006 Springer
    Dominic W.S. Wong.
  • 2007 Springer
    by Paul S. Agutter and Denys N. Wheatley.
    Ingredients of the simplest cells (prokaryotes and the sizes of their contents) -- Bigger cells (eukaryotic cells and their contents) -- Hives of industry (a survey of intermediary metabolism) -- Delights of transport (how the cell's contents are moved around) -- As if standing still (cellular homeostasis and regulatory processes) -- Internal state and gene expression (transcription and its control) -- Sustaining and changing the internal state (the interrelationship between gene expression and the cell's current composition and functional state) -- Responding to the environment (signal processing, gene expression and internal state) -- The living state (a characterization of 'life') -- Stability and change in DNA (how genes can be altered) -- The spice of life (diversity, natural selection and symbiosis) -- Curriculum vitae (an outline history of life on Earth) -- The origin of life (some major ideas and unanswered questions) -- Other worlds (the possibility of extraterrestrial life) -- Intelligent behavior and brains (the biological meaning of "intelligence") -- Human evolution (human intelligence and the question of human uniqueness) -- Cells, brains and computers: towards a characterisation of mind.
  • 2011 Springer Protocols
    edited by Richard O. Snyder, Philippe Moullier.
    Adeno-associated virus biology / Matthew D. Weitzman and R. Michael Linden -- Design and construction of functional AAV vectors / John T. Gray and Serge Zolotukhin -- AAV capsid structure and cell interactions / Mavis Agbandje-McKenna and Jürgen Kleinschmidt -- Exploring natural diversity of AAV for the design of vectors with novel properties / Guangping Gao, Li Zhong, and Olivier Danos -- Gene therapy in skeletal muscle mediated by adeno-associated virus vectors / Chunping Qiao ... [et al.] -- AAV mediated liver-directed gene therapy / Mark S. Sands -- Recombinant AAV delivery to the central nervous system / Olivier Bockstael ... [et al.] -- Adeno-associated virus mediated gene therapy for retinal degenerative diseases / Knut Steiger ... [et al.] -- Adeno-associated virus vector delivery to the heart / Lawrence T. Bish ... [et al.] -- Evaluation of the fate of rAAV genomes following in vivo administration / K. Reed Clark and Magalie Penaud-Budloo -- Measuring immune responses to recombinant AAV gene transfer / Ashley T. Martino ... [et al.] -- Modificaton and labeling of AAV vector particles / Hildegard Büning ... [et al.] -- AAV mediated gene targeting / Daniel G. Miller -- Preclinical study design for rAAV / Terence R. Flotte, Thomas J. Conlon, and Christian Mueller -- Biodistribution and shedding of AAV vectors / Caroline Le Guiner, Phillipe Moullier, and Valder R. Arruda -- Production and purification of recombinant adeno-associated vectors / Lijun Wang ... [et al.] -- rAAV vector product characterization and stability studies / Richard O. Snyder, Muriel Audit, and Joyce D. Francis -- rAAV human trial experience / Katherine A. High and Patrick Aubourg.
  • 2012 Springer
    Charles E. Samuel, editor.
    ADAR proteins : structure and catalytic mechanism / Rena A. Goodman, Mark R. Macbeth and Peter A. Beal -- ADAR proteins : double-stranded RNA and Z-DNA binding domains / Pierre Barraud and Frédéric H.-T. Allain -- Editing of neurotransmitter receptor and ion channel RNAs in the nervous system / Jennifer L. Hood and Ronald B. Emeson -- Modulation of microRNA expression and function by ADARs / Bjorn-Erik Wulff and Kazuko Nishikura -- Nuclear editing of mRNA 3'-UTRs / Ling-Ling Chen and Gordon G. Carmichael -- Control of ADAR1 editing of hepatitis delta virus RNAs / John L. Casey -- Bioinformatic approaches for identification of A-to-I editing sites / Eli Eisenberg -- ADARs : viruses and innate immunity / Charles E. Samuel -- Role of ADARs in mouse development / Carl R. Walkley, Brian Liddicoat and Jochen C. Hartner -- Regulation and functions of ADAR in Drosophila / Simona Paro, Xianghua Li, Mary A. O'Connell and Liam P. Keegan.
  • v. 1-2, 2006. Springer Protocols
    v. 2, 2006 Springer Protocols
    edited by William S.M. Wold, Ann E. Tollefson.
    v. 1. Adenoviruses, ad vectors, quantitation, and animal models -- v. 2. Ad proteins and RNA, lifecycle and host interactions, and phylogenetics.
  • 2010 CRCnetBASE
    editor, Victor R. Preedy.
    Adhesion molecules are a family of proteins located on the cell surface and are involved in cell-to-cell or cell-extracellular matrix binding. They have a role in maintaining the cellular integrity of tissues and the functional viability of organs. In many diseases the normative features of adhesion molecules are perturbed. Thus, understanding how adhesion molecules behave in health and disease is a key step in the advancement of science and health in general. In the first part of the hook, chapters cover the structure and classification of adhesion molecules, immunohistochemical localization, junctional and inflammatory adhesion molecules and molecular imaging. The other four parts of the book cover adhesion molecules in relation to signalling pathways, gene expression, arginine, stem cells, neutrophil migration, leukocytes, decompression sickness, ischemia reperfusion injury, diabetes, obesity, metabolic syndrome, weight loss, hypoxia, kidney disease, smoking, osteoprotegerin, tumors, biointormatics, high-throughput technologies, chemotherapy, hematopoiesis, lipoproteins, fatty acids, atrial fibrillation and heart disease, brain and dementia. The book is an essential book for research scientists, pathologists, molecular biologists, biochemists or cellular biochemists, clinicians, health care professionals, general practitioners as well as those interested in disease in general.
  • 2007 Springer
    Klaus Ley, editor.
    Also available: Print – 2007
  • 2010 Springer
    edited by Simon Yona, Martin Stacey.
    The adhesion GPCRs : gene repertoire, phylogeny, and evolution / Helgi B. Schith, Karl J.V. Nordstrom, and Robert Fredriksson -- Cadherins : developmental roles and future challenges / Caroline J. Formstone -- Latrophilin signalling in tissue polarity and morphogenesis / Tobias Langenhan and Andreas P. Russ -- GPS proteolytic cleavage of adhesion-GPCRs / Hsi-Hsien Lin ... [et al.] -- The latrophilins : "split-personality" receptors / John-Paul Silva and Yuri A. Ushkaryov -- Studies on the very large G protein-coupled-receptor : from initial discovery to determining its role in sensorineural deafness in higher animals / D. Randy McMillan and Perrin C. White -- Adhesion-GPCRs in the CNS / Natalie Strokes and Xianhua Piao -- GPR56 interacts with extracellular matrix and regulates cancer progression / Lei Xu -- Adhesion-GPCRs in tumorigenesis / Gabriela Aust -- Immunity and adhesion-GPCRs / Simon Yona, Hsi-Hsien Lin, and Martin Stacey -- CD97 in leukocyte trafficking / Jorg Hamann ... [et al.] -- The role of CD97 in regulating adaptive T-cell responses / Ian Spendlove and Ruhcha Sutavani -- F4/80 : the macrophage specific adhesion-GPCR and its role in immunoregulation / Hsi-Hsien Lin ... [et al.] -- Signal transduction mediated through adhesion-GPCRs / Norikazu Mizuno and Hiroshi Itoh -- Emerging roles of brain specific angiogenesis inhibitor 1 / Daeho Park and Kodi S. Ravichandran -- Adhesion-GPCRs in the male reproductive tract / Ben Davies and Christiane Kirchhoff.
    Also available: Print – 2010
  • 2011 Springer Protocols
    edited by Jeffrey M. Gimble, Bruce A. Bunnell.
    Isolation and culture of rhesus adipose-derived stem cells -- Isolation of human adipose-derived stem cells from lipoaspirates -- Isolation of murine adipose-derived stem cells -- In vitro adult rat adipose tissue-derived stromal cell isolation and differentiation -- In vitro adult canine adipose tissue-derived stromal cell growth characteristics -- Adipogenic differentiation of adult equine mesenchymal stromal cells -- Isolation and culture of porcine adipose tissue-derived somatic stem cells -- Automated isolation and processing of adipose-derived stem and regenerative cells -- Methods for the purification and characterization of human adipose-derived stem cells -- Characterization of human adipose-derived stem cells using flow cytometry -- Evaluation of cellular and humoral immune responses to allogeneic adipose-derived stem/stromal cells -- Adipose-derived stromal cells: Cytokine expression and immune cell contaminants -- Gel-based and gel-free proteomic technologies -- Adipogenic differentiation of adipose-derived stem cells -- Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells -- Neural differentiation of human adipose tissue-derived stem cells -- Osteogenic differentiation strategies for adipose-derived mesenchymal stem cells -- Generation of adipose stromal cell-derived hepatic cells -- Adipose stem cell differentiation into smooth muscle cells -- Endothelial and cardiac regeneration from adipose tissues -- Epithelial differentiation of human adipose-derived stem cells -- Screening for epigenetic target genes that enhance reprogramming using lentiviral-delivered shRNA -- Adipogenic differentiation of human adipose-derived stem cells on 3D silk scaffolds -- A first approach for the production of human adipose tissue-derived stromal cells for therapeutic use -- Viral transduction of adipose-derived stem cells -- Use of adipose-derived stem cells in high-throughput screening to identify modulators of lipogenesis -- Preservation protocols for human adipose tissue-derived adult stem cells -- Soft tissue reconstruction -- Methods for analyzing microRNA expression and function during osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells -- The generation and the manipulation of human multipotent adipose-derived stem cells -- Cell sheet technology for tissue engineering: The self-assembly approach using adipose-derived stromal cells -- Differentiation of adipose-derived stem cells for tendon repair -- Adipose-derived stem cells for skin regeneration -- Adipose-derived stem cells for periodontal tissue regeneration.
  • 2007 Springer
    edited by Matthew A. Jenks, Paul M. Hasegawa and S. Mohan Jain.
  • 2007 Springer
    Febrizio d'Adda di Fagagna ... [et al.], editors ; edited under the auspices of the the European Institute of Oncology ... and the FIRC Institute of Molecular Oncolgy Foundation.
    Genome instability, checkpoints, and the cell cycle. Comparison of DNA replication in Xenopus laevis and simian virus 40 / Maren Oehlmann, Cathal Mahon, and Heinz-Peter Nasheuer. A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors / P. Mathijs Voorhoeve ... [et al.] An investigation into 53BP1 complex formation / Kevin C. Roche and Noel F. Lowndes. Short abstracts--session I -- Tumor and microenvironment interactions. Tumor promotion by tumor-associated macrophages / Chiara Porta ... [et al.] The AP-2[alpha] transcription factor regulates tumor cell migration and apoptosis / Francesca Orso ... [et al.] Modulatory actions of neuropeptide Y on prostate cancer growth: role of MAP kinase/ERK 1/2 activation / Massimiliano Ruscica ... [et al.] Short abstracts--session II -- Animal models. The Arf tumor suppressor in acute leukemias: insights from mouse models of Bcr-Abl-induced acute lymphoblastic leukemia / Richard T. Williams and Charles J. Sherr. Short abstracts--session III -- Transcription and epigenetics. Short abstracts--session IV -- High-throughput approaches and imaging. Identification and validation of the anaplastic large cell lymphoma signature / Roberto Piva, Elisa Pellegrino, and Giorgio Inghirami. Cell-cycle inhibitor profiling by high-content analysis / Fabio Gasparri, Antonella Ciavolella, and Arturo Galvani. Short abstracts-session V -- Novel pathways and therapeutic targets. Regulation for nuclear targeting of the Abl tyrosine kinase in response to DNA damage / Kiyotsugu Yoshida. Short abstracts--session VI -- Poster abstracts.
    Also available: Print – 2007
  • 2008 Springer Protocols
    edited by Michael Zachariou.
    Affinity chromatography : history, perspectives, limitations, and prospects / Ana Cecília A. Roque and Christopher R. Lowe -- Immobilized metal ion affinity chromatography of native proteins / Adam Charlton and Michael Zachariou -- Affinity precipitation of proteins using metal chelates / Ashok Kumar, Igor Yu. Galaev, and Bo Mattiasson -- Immunoaffinity chromatography / Stuart R. Gallant, Vish Koppaka, and Nick Zecherle -- Dye ligand chromatography / Stuart R. Gallant, Vish Koppaka, and Nick Zecherle -- Purification of proteins using displacement chromatography / Nihal Tugcu -- Rationally designed ligands for use in affinity chromatography : an artificial protein L / Ana Cecília A. Roque and Christopher R. Lowe -- Phage display of peptides in ligand selection for use in affinity chromatography / Joanne L. Casey, Andrew M. Coley, and Michael Foley -- Preparation, analysis, and use of an affinity adsorbent for the purification of GST fusion protein / Gareth M. Forde -- Immobilized metal ion affinity chromatography of histidine-tagged fusion proteins / Adam Charlton and Michael Zachariou -- Methods for the purification of HQ-tagged proteins / Becky Godat ... [et al.] -- Amylose affinity chromatography of maltose-binding protein : purification by both native and novel matrix-assisted dialysis refolding methods / Leonard K. Pattenden and Walter G. Thomas -- Methods for detection of protein-protein and protein-DNA interactions using HaloTag / Marjeta Urh ... [et al.] -- Site-specific cleavage of fusion proteins / Adam Charlton -- The use of TAGZyme for the efficient removal of N-terminal his-tags / José Arnau ... [et al.] -- Affinity processing of cell-containing feeds using monolithic macroporous hydrogels, cryogels / Igor Yu. Galaev and Bo Mattiasson -- Monolithic bioreactors for macromolecules / Mojca Benčina ... [et al.] -- Plasmid DNA purification via the use of a dual affinity protein / Gareth M. Forde -- Affinity chromatography of phosphorylated proteins / Grigoriy S. Tchaga -- Protein separation using immobilized phospholipid chromatography / Tzong-Hsien Lee and Marie-Isabel Aguilar -- Analysis of proteins in solution using affinity capillary electrophoresis / Niels H.H. Heegaard, Christian Schou, and Jesper Østergaard.
  • 2015 Springer Protocols
    edited by Senta Reichelt, Leibniz-Institut für Oberflächenmodifizierung, Leipzig, Germany.
    Affinity chromatography : a historical perspective -- Protein purification by amniosquarylium cyanine dye-affinity chromatography -- One-step purification of phosphinothricin acetyltransferase using reactive dye-affinity chromatography -- Antibody purification from human plasma by metal-chelated affinity membranes -- Specific recognition of supercoiled plasmid DNA by affinity chromatography using a synthetic aromatic ligand -- Cell affinity separations on microfluidic devices -- Aptamer-modified magnetic beads in affinity separation of proteins -- The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture -- Robotic high-throughput purification of affinity-tagged recombinant proteins -- Macroporous silica particles derivatized for enhanced lectin affinity enrichment of glycoproteins -- Immobilized magnetic beads-based multi-target affinity selection coupled with HPLC-MS for screening active compounds from traditional Chinese medicine and natural products -- Mixed-bed affinity chromatography : principles and methods -- Preparation and characterization of fluorophenylboronic acid-functionalized affinity monolithic columns for the selective enrichment of cis-diol-containing biomolecules -- Introduction to macroporous cryogels -- Cryogels with affinity ligands as tools in protein purification -- Direct capture of His₆-tagged proteins using megaporous cryogels developed for metal-ion affinity chromatography -- Ni(II) chelated IDA functionalized poly(HEMA-GMA) cryogels for urease adsorption -- A novel chromatographic media : histidine-containing composite cryogels for HIgG separation from human serum -- Molecularity imprinted cryogels for human serum albumin depletion -- Interpenetrating polymer network composite cryogels with tailored porous morphology and sorption properties -- Analysis of drug-protein interactions by high-performance affinity chromatography : interactions of sulfonylurea drugs with normal and glycated human serum albumin -- Accurate protein-peptide titration experiments by nuclear magnetic resonance using low-volume samples -- Characterization of the binding strengths between boronic acids and cis-diol-containing biomolecules by affinity capillary electrophoresis -- Determination if the kinetic rate constant of cyclodextrin supramolecular systems by high-performance affinity chromatography -- Molecular modeling of the affinity chromatography of monoclonal antibodies.
  • v. 1-2, 2006. Springer Protocols
    v. 2, 2006 Springer Protocols
    edited by Kan Wang.
  • 2015 Springer Protocols
    Fulltext v. 2 (2015) Springer Protocols
    edited by Kan Wang, Center for Plant Transformation, Plant Sciences Institute, and Department of Agronomy, Iowa State University, Ames, IA, USA.
    Volume 1. Gateway®-compatible plant transformation vectors /Mark A. Smedley and Wendy A. Harwood -- Brachypodium distachyon /Jennifer N. Bragg ... [et al.] -- Medicago truncatula transformation using cotyledonary explants /Elane Wright and Zeng-Yu Wang -- Medicago truncatula transformation using leaf explants /Viviane Cosson, Alexis Eschstruth , and Pascal Ratet -- Setaria viridis /Joyce Van Eck and Kerry Swartwood -- Barley (Hordeum vulgare L.) transformation using immature embryos /Cornelia Marthe, Jochen Kumlehn, and Goetz Hensel -- Barley (Hordeum vulgare L.) transformation using embryogenic pollen cultures /Ingrid Otto, Andrea Müller, and Jochen Kumlehn -- Maize (Zea mays L.) /Bronwyn Frame ... [et al.] -- Maize, tropical (Zea mays L.) /Shireen K. Assem -- Finger millet [Eleusine coracana (L.) Gaertn.] /Stanislaus Antony Ceasar and Savarimuthu Ignacimuthu -- Oat (Avena sativa L.) /Sebastian Gasparis and Anna Nadolska-Orczyk -- Rice, indica (Oryza sativa L.) /Yukoh Hiei, Yuji Ishida, and Toshihiko Komari -- Rice, japonica (Oryza sativa L.) /Marcy Main, Bronwyn Frame, and Kan Wang -- Sorghum (sorghum bicolor ) /Xiaomei Guo, Zhengxiang Ge, Shirley J. Sato, and Tom E. Clemente -- Wheat (Triticum aestivum L.) transformation using immature embryos /Yuji Ishida ... [et al.] -- Wheat (Triticum aestivum L.) transformation using mature embryos /Eva Medvecká and Wendy A. Harwood -- Alfalfa (Medicago sativa L.) /Chunxiang Fu ... [et al.] -- Clovers (Trifolium spp.) /Samira Rahimi-Ashtiani ... [et al.] -- Clover, red (Trifolium pratense) /Michael L. Sullivan and Kenneth H. Quesenberry -- Cowpea [Vigna unguiculata (L.) walp.] /Ratikanta Behura ... [et al.] -- Lentil (Lens culinaris medik) /Ufuk Celikkol Akcay, Meral Yücel, and Hüseyin Avni Öktem -- Soybean [Glycine max (L.) Merr.] /Diane Luth, Katey Warnberg, and Kan Wang -- Brassica oleracea and B. napus /Penny A. C. Hundleby née Sparrow and Judith A. Irwin -- Cucumber (Cucumis sativus L.) and kabocha squash (Cucurbita moschata Duch) /Yoshihiko Nanasato and Yutaka Tabei -- Pepper, chili (Capsicum annuum) /Jung Min ... [et al.] -- Pepper, sweet (Capsicum annuum) /Iris Heidmann and Kim Boutilier -- Sugar beet (Beta vulgaris L.) /Hiroyo Kagami ... [et al.] -- Tomato (Solanum lycopersicum) /Dora Garcia, Javier Narváez-Vásquez, and Martha L. Orozco-Cárdenas.
  • 2008 Springer Protocols
    Laura E. Nagy, editor.
    Acute models of ethanol exposure to mice / Timothy P. Plackett and Elizabeth J. Kovacs -- A voluntary oral-feeding rat model for pathological alcoholic liver injury / George L. Tipoe ... [et al.] -- Intragastric ethanol infusion model in rodents / Hide Tsukamoto, Hasmik Mkrtchyan, and Alla Dynnyk -- A practical method of chronic ethanol administration in mice / Ruth A. Coleman ... [et al.] -- Analysis of ethanol developmental toxicity in zebrafish / Robert L. Tanguay and Mark J. Reimers -- The avian embryo in fetal alcohol research / Susan M. Smith -- Artificial rearing / Hector D. Dominguez and Jennifer D. Thomas -- Intragastric intubation of alcohol during the perinatal period / Sandra J. Kelly and Charles R. Lawrence -- Human monocytes, macrophages, and dendritic cells : alcohol treatment methods / Gyongyi Szabo and Pranoti Mandrekar -- Generation and use of primary rat cultures for studies of the effects of ethanol / Amanda Lindke, Barbara Tremper-Wells, and Michael W. Miller -- Development and properties of HepG2 cells that constitutively express CYP2E1 / Defeng Wu and Arthur I. Cederbaum -- Modeling the impact of alcohol on cortical development in a dish : strategies from mapping neural stem cell fate / Rajesh C. Miranda ... [et al.] -- Acetaldehyde-induced barrier disruption and paracellular permeability in Caco-2 cell monolayer / R.K. Rao -- Alcohol-induced oxidative stress in the liver : in vivo measurements / Gavin E. Arteel -- Isolation of Kupffer cells from rats fed chronic ethanol / Megan R. McMullen, Michele T. Pritchard, and Laura E. Nagy -- Dendritic cells in chronic in vivo ethanol exposure models / Kevin L. Legge and Annette J. Schlueter -- Formation and immunological properties of aldehyde-derived protein adducts following alcohol consumption / Geoffrey M. Thiele, Lynell W. Klassen, and Dean J. Tuma -- Assessment of natural killer (NK) and NKT cells in murine spleens and livers / Michael R. Shey and Zuhair K. Ballas -- Polyclonal and antigen-specific responses of T cells and T cell subsets / Betty M. Young ... [et al.] -- B-cell studies in chronic ethanol mice / Shilpi Verma ... [et al.] -- Histological analysis of bone / Urszula T. Iwaniec, Thomas J. Wronski, and Russell T. Turner -- Assessing effects of alcohol consumption on protein synthesis in striated muscles / Thomas C. Vary and Charles H. Lang -- Methods to investigate the effects of chronic ethanol on adipocytes / Becky M. Sebastian ... [et al.] -- Proteomic approaches to identify and characterize alterations to the mitochondrial proteome in alcoholic liver disease / Shannon M. Bailey ... [et al.] -- Alcoholic liver disease and the mitochondrial ribosome : methods of analysis / Alan Cahill and Peter Sykora -- Microarray analysis of ethanol-induced changes in gene expression / Robnet T. Kerns and Michael F. Miles.
  • 2010
    Brandon E. Johnson.
    Ion channels coordinate the movement of ions across biological membranes in response to a diversity of cellular signals to regulate most if not all aspects of physiology. The large-conductance Ca2+- and voltage-activated potassium (BK) channel is widely expressed across tissues and functions to repolarize action potentials, regulate smooth muscle tone and endocrine function, and tune auditory hair cells. In all tissues, BK channels provide a functional link between cytosolic Ca2+ and membrane excitation. Extensive alternate splicing of the slo-1 gene, encoding the BK channel, significantly diversifies channel function. We identified all twelve predicted BK channel splice variants expressed by the nematode Caenorhabditis elegans, and measured the Ca2+ and voltage dependence for each. Alternate exons encode portions of two regions of the cytoplasmic tail: the RCK domain, important for Ca2+ coordination, and an unstructured region, termed the C-linker. Using the patch clamp technique, we determined that alternate splicing across multiple sites diversifies activation kinetics and Ca2+ sensitivity by manipulating the functional interaction between the RCK domain and C-linker. We recovered two slo-1 alleles (pg34 and pg52) that contain point-mutations near an alternative splice site in the C-linker from a mutant screen. Using real-time PCR, we determined that both mutations change slo-1 splice variant expression profiles. The pg34 mutation encodes an A698T substitution, and the pg52 mutation is located in an intron. When heterologously expressed, the A698T mutation changes BK channel Ca2+ sensitivity in specific splice variant backgrounds by disrupting the functional connection between the C-linker and the RCK domain. Using in vivo electrophysiology, we determined that the pg34 mutation reduces but does not abolish BK channel function in C. elegans neurons. These results suggest that alternate splicing of slo-1 modulates the functional connection between the RCK domain and the C-linker of the BK channel to establish a proper balance between intracellular Ca2+ and membrane excitation in a variety of cell type and physiological conditions.
  • 2006 Springer
    Philippe Jeanteur (ed.).
    Methods and platforms for the quantification of splice variants' expression / Laurent Bracco ... [et al.] -- Pre-mRNA missplicing as a cause of human disease / Tatyana Novoyatleva ... [et al.] -- Alternative splicing : therapeutic target and tool / Mariano A. Garcia-Blanco -- SR proteins as potential targets for therapy / Johann Soret, Mathieu Gabut, Jamal Tazi -- Misregulation of tau alternative splicing in neurodegeneration and dementia / Athena Andreadis -- Spinal muscular atrophy and therapeutic prospects / Brunhilde Wirth, Lars Brichta, Eric Hahnen -- Misregulation of alternative splicing causes pathogenesis in myotonic dystrophy / N. Muge Kuyumcu-Martinez, Thomas A. Cooper -- Redirecting splicing to address dystrophin mutations : molecular by-pass surgery / Stephen D. Wilton, Susan Fletcher -- Altered splicing in prelamin A-associated premature aging phenotypes / Annachiara De Sandre-Giovannoli, Nicolas Levy -- Splicing modulation as a modifier of the CFTR function / Malka Nissim-Rafinia, Batsheva Kerem.
  • 2010 Springer Protocols
    edited by Erik D. Roberson.
    Contemporary Approaches to Alzheimer's Disease and Frontotemporal Dementia / Erik D. Roberson -- Preparing Synthetic Aβ in Different Aggregation States / W. Blaine Stine, Lisa Jungbauer, Chunjiang Yu, Mary Jo LaDu -- Isolation of Low-n Amyloid β-Protein Oligomers from Cultured Cells, CSF, and Brain / Ganesh M. Shankar, Alfred T. Welzel, Jessica M. McDonald, Dennis J. Selkoe, Dominic M. Walsh -- Detecting Aβ*56 Oligomers in Brain Tissues / Mathew A. Sherman, Sylvain E. Lesné -- Assessing Aβ Aggregation State by Atomic Force Microscopy / Justin Legleiter -- Measuring APP Carboxy-Terminal Fragments / Luke A. Esposito -- Detection of APP Intracellular Domain in Brain Tissue / Sanjay W. Pimplikar, Anupama Suryanarayana -- Cell-Based Assays for Regulators of Tau Biology / Umesh K. Jinwal, Chad A. Dickey -- Split GFP Complementation Assay for Quantitative Measurement of Tau Aggregation In Situ / Wanjoo Chun, Geoffrey S. Waldo, Gail V. Johnson -- Apolipoprotein E Expression and Purification / Yvonne Newhouse, Karl H. Weisgraber -- Aβ Toxicity in Primary Cultured Neurons / Adriana Ferreira, Roxana C. Sinjoanu, Alexandra Nicholson, Sara Kleinschmidt -- Manipulation of Gene Expression in the Central Nervous System with Lentiviral Vectors / Binggui Sun, Li Gan -- Selecting a Mouse Model of Alzheimer's Disease / Jeannie Chin -- Monitoring Spatial Learning and Memory in Alzheimer's Disease Mouse Models Using the Morris Water Maze / Kimberly Scearce-Levie -- Step-by-Step In Situ Hybridization Method for Localizing Gene Expression Changes in the Brain / Jorge J. Palop, Erik D. Roberson, Inma Cobos -- Real-Time Visualization of Axonal Transport in Neurons / Yasuko Osakada, Bianxiao Cui -- Quantifying Biomarkers of Cognitive Dysfunction and Neuronal Network Hyperexcitability in Mouse Models of Alzheimer's Disease: Depletion of Calcium-Dependent Proteins and Inhibitory Hippocampal Remodeling / Jorge J. Palop, Lennart Mucke, Erik D. Roberson -- Epigenetic Changes in the Brain: Measuring Global Histone Modifications / Gavin Rumbaugh, Courtney A. Miller.
  • 2012 Springer Protocols
    edited by Einar M. Sigurdsson, Miguel Calero, María Gasset.
    Rapid generation of dityrosine cross-linked A-[beta] oligomers via cu-redox cycling / Adam P. Gunn, Blaine R. Roberts, and Ashley I. Bush -- Application of photochemical cross-linking to the study of oligomerization of amyloidogenic proteins / Dahabada H.J. Lopes [and others] -- Preparation of stable amyloid b-protein oligomers of defined assembly order / Clark Rosensweig [and others] -- Purification and fibrillation of full-length recombinant PrP / Natallia Makarava and Ilia V. Baskakov -- Featuring amyloids with fourier transform infrared and circular dichroism spectroscopies / Miguel Calero and María Gasset -- Quasielastic light scattering study of amyloid b-protein fibrillogenesis / Aleksey Lomakin and David B. Teplow -- Conformations of microtubule-associated protein tau mapped by fluorescence resonance energy transfer / Sadasivam Jeganathan [and others] -- Measuring the kinetics of amyloid fibril elongation using quartz crystal microbalances / Alexander K. Buell, Christopher M. Dobson, and Mark E. Welland -- X-ray fibre diffraction studies of amyloid fibrils / Kyle L. Morris and Louise C. Serpell -- Structural characterization of prefibrillar intermediates and amyloid fibrils by small-angle x-ray scattering / Annette Eva Langkilde and Bente Vestergaard -- Atomic force fluorescence microscopy in the characterization of amyloid fibril assembly and oligomeric intermediates / Valeriy Ostapchenko, Maria Gasset, and Ilia V. Baskakov -- Investigating fibrillar aggregates of tau protein by atomic force microscopy / Susanne Wegmann, Daniel J. Muller, and Eckhard Mandelkow -- Structural studies of amyloids by quenched hydrogen-deuterium exchange by NMR / Marçal Vilar, Lei Wang, and Roland Riek -- Cyclic amplification of prion protein misfolding / Marcelo A. Barria, Dennisse Gonzalez-Romero, and Claudio Soto -- Search for amyloid-binding proteins by affinity chromatography / Miguel Calero, Agueda Rostagno, and Jorge Ghiso -- Establishing the links between A[beta] aggregation and cytotoxicity in vitro using biophysical approaches / Asad Jan and Hilal A. Lashuel -- Preparation of cultured human vascular cells / Ingvar H. Olafsson, Dadi Th. Vilhjalmsson, and Finnbogi R. Thormodsson -- Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy : isolation of smooth muscle and endothelial cells from mouse brain / Sebastien A. Gauthier [and others] -- In vitro assays measuring protection by proteins such as cystatin C of primary cortical neuronal and smooth muscle cells / Sebastien A. Gauthier [and others] -- Study of neurotoxic intracellular calcium signalling triggered by amyloids / Carlos Villalobos [and others] -- Bacterial amyloids / Yizhou Zhou [and others] -- Study of amyloids using yeast / Reed B. Wickner [and others] -- Cell-to-cell transmission of [alpha]-synuclein aggregates / Seung-Jae Lee [and others] -- Subcutaneous adipose tissue biopsy for amyloid protein studies / Per Westermark -- Analysis of s100 oligomers and amyloids / Hugo M. Botelho, Günter Fritz, and Cláudio M. Gomes -- S100A8/A9 amyloidosis in the ageing prostate : relating ex vivo and in vitro studies / Anna L. Gharibyan, Dina Raveh, and Ludmilla A. Morozova-Roche -- Isolation of amyloid by solubilization in water / Dadi Th. Vilhjalmsson, Indiana E. Ingolfsdottir, and Finnbogi R. Thormodsson -- Histological staining of amyloid and pre-amyloid peptides and proteins in mouse tissue / Hameetha B. Rajamohamedsait and Einar M. Sigurdsson -- Pentameric luminescent-conjugated oligothiophene for optical imaging of in vitro-formed amyloid fibrils and protein aggregates in tissue sections / K. Peter R. Nilsson, Mikael Lindgren, and Per Hammarström -- In vivo magnetic resonance imaging of amyloid-[beta] plaques in mice / Youssef Zaim Wadghiri [and others] -- Mouse model for scrapie : inoculation, clinical scoring, and histopathological techniques / Michele A. Di Bari, Romolo Nonno, and Umberto Agrimi -- Biochemical isolation of insoluble tau in transgenic mouse models of tauopathies / Carl Julien, Alexis Bretteville, and Emmanuel Planel -- Tissue processing prior to analysis of alzheimer's disease associated proteins and metabolites, including A[beta] / Stephen D. Schmidt, Ralph A. Nixon, and Paul M. Mathews -- A-[beta] measurement by enzyme-linked immunosorbent assay / Stephen D. Schmidt [and others] -- Cognitive and sensorimotor tasks for assessing functional impairments in mouse models of Alzheimer's Disease and related disorders / Allal Boutajangout [and others].
  • 2010
    Adrienne Orr.
    Alzheimer's disease (AD) is a progressive neurodegenerative disorder affecting the elderly that is characterized by loss of short-term memory and cognitive decline. Studies in both human patients and animal models of AD point to over-accumulation of soluble oligomers of amyloid-beta peptide (Abeta) as a mediator of learning and memory impairments early in the disease (Rowan, Klyubin et al. 2005; Selkoe 2008). Support for Abeta's role in learning deficits comes from studies demonstrating impaired plasticity of synaptic inputs to neurons, as measured in the paradigm of long-term potentiation (LTP) of synaptic strength in hippocampal slices (Chen, Kagan et al. 2000; Freir, Holscher et al. 2001; Walsh, Klyubin et al. 2002; Wang, Pasternak et al. 2002; Townsend, Shankar et al. 2006), the prime candidate for the neuronal substrate of learning and memory behavior (Bliss and Collingridge 1993). Induced concurrently with synaptic LTP is an increase in the electrical coupling between the dendritic synaptic inputs and the soma, such that a greater proportion of the EPSP survives at the spike trigger zone, resulting in greater action potential output for a given EPSP. This potentiation of EPSP-spike coupling (E-S coupling) provides an additional boost to the efficacy of the EPSP on top of the potentiation (LTP) that occurs at the synapse (Bliss and Lomo 1973). While LTP of synaptic strength is an essential component of memory storage, in all models of learning, memory recall occurs only when spikes are evoked in the neurons participating in a representation. Thus E-S potentiation exerts control over the essential substrate of memory retrieval. Although LTP and E-S potentiation are mechanistically distinct processes, several features during the induction phase are shared. Studies have shown that Abeta impairs the induction, but not maintenance of LTP (Wang, Walsh et al. 2004; Townsend, Shankar et al. 2006), leaving the possibility that Abeta-mediated impairment to memory is not specific to LTP. Nevertheless, focus has centered on pathways central to LTP, while impairment to other forms of plasticity have not been explored. Here we show a mechanism by which Abeta peptides impairs post-tetanic suppression of inhibition, thereby inhibiting E-S potentiation. This impairment to E-S potentiation was occluded by GABAA-receptor, Cannabinoid 1 receptor (CB1R), and muscarinic acetylcholine receptor (mAChR), but not mGluR block. This suggests that the impairment is mediated by mAChR-initiated endocannbinoid signaling. Together, the results provide a mechanism to explain learning and memory impairments in early Alzheimer's disease.
  • 2011 Springer
    by Rajan Katoch.
    Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.
  • 2012 Springer Protocols
    edited by Beth Shapiro, Michael Hofreiter.
    Setting up an ancient DNA laboratory / Tara L. Fulton -- A phenol-chloroform protocol for extracting DNA from ancient samples / Ross Barnett and Greger Larson -- DNA extraction of ancient animal hard tissue samples via adsorption to silica particles / Nadin Rohland -- Case study : recovery of ancient nuclear DNA from toe pads of the extinct passenger pigeon / Tara L. Fulton, Stephen M. Wagner, and Beth Shapiro -- Extraction of DNA from paleofeces / Melanie Kuch and Hendrik Poinar -- DNA extraction from keratin and chitin / Paula F. Campos and Thomas M.P. Gilbert -- Case study : ancient sloth DNA recovered from hairs preserved in paleofeces / Andrew A. Clack, Ross D.E. MacPhee, and Hendrik N. Poinar -- Ancient DNA extraction from soils and sediments / James Haile -- DNA extraction from fossil eggshell / Charlotte L. Oskam and Michael Bunce -- Ancient DNA extraction from plants / Logan Kistler -- DNA extraction from formalin-fixed material / Paula F. Campos and Thomas M.P. Gilbert -- Case study : ancient DNA recovered from Pleistocene-age remains of a Florida armadillo / Brandon Letts and Beth Shapiro -- Nondestructive DNA extraction from museum specimens / Michael Hofreiter -- Case study : using a nondestructive DNA extraction method to generate mtDNA sequences from historical chimpanzee specimens / Elmira Mohandesan, Stefan Prost, and Michael Hofreiter -- PCR amplification, cloning, and sequencing of ancient DNA / Tara L. Fulton and Mathias Stiller -- Quantitative real-time PCR in aDNA research / Michael Bunce, Charlotte L. Oskam, and Morten E. Allentoft -- Multiplex PCR amplification of ancient DNA / Mathias Stiller and Tara L. Fulton -- Preparation of next-generation sequencing libraries from damaged DNA / Adrian W. Briggs and Patricia Heyn -- Generating barcoded libraries for multiplex high-throughput sequencing / Michael Knapp, Mathias Stiller, and Matthias Meyer -- Case study : targeted high-throughput sequencing of mitochondrial genomes from extinct cave bears via direct multiplex PCR sequencing (DMPS) / Mathias Stiller -- Target enrichment via DNA hybridization capture / Susanne Horn -- Case study : enrichment of ancient mitochondrial DNA by hybridization capture / Susanne Horn -- Analysis of high-throughput ancient DNA sequencing data / Martin Kircher -- Phylogenetic analysis of ancient DNA using BEAST / Simon Y.W. Ho.
  • 2014 Springer Protocols
    edited by Andreas Werner.
    Targeted small noncoding rna-directed gene activation in human cells -- Isolation of chromatoid bodies from mouse testis as a rich source of short RNAs -- Generation of endo-siRNAs in xenopus laevis oocytes -- Analysis of endo-siRNAs in drosophila -- Methods for studying the biological consequences of endo-siRNA deficiency in drosophila melanogaster -- Small RNA library cloning procedure for deep sequencing of specific endogenous siRNA classes in caenorhabditis elegans -- Assays for direct and indirect effects of c. Elegans endo-siRNAs -- Extraction and nonradioactive detection of small RNA molecules -- P19-mediated enrichment and detection of siRNAs -- Detection of small noncoding RNAs by in situ hybridization using probes of 2'-o-methyl RNA + LNA -- Enhanced detection of small RNAs using a nonradioactive approach -- Computing siRNA and piRNA overlap signatures -- Isolation of small interfering RNAs using viral suppressors of RNA interference -- Computational analysis, biochemical purification, and detection of tRNA-derived small RNA fragments -- Differential DNA methylation patterns in endo-siRNAs mediated silencing of LINE-1 retrotransposons.
  • 2008 CRCnetBASE
    edited by Stanley T. Crooke.
    Mechanisms of antisense drug action, an introduction / Stanley T. Crooke ... [et al.] -- The RNase H mechanism / Walt Lima, Hongjiang Wu, and Stanley T. Crooke -- Small RNA silencing pathways / Alla Sigova and Phillip D. Zamore -- Splice switching oligonucleotides as potential therapeutics / Peter Sazani, Maria A. Graziewicz, and Ryszard Kole -- Basic principles of antisense drug discovery / Susan M. Freier and Andrew T. Watt -- The medicinal chemistry of oligonucleotides / Eric E. Swayze and Balkrishen Bhat -- Basic principles of the pharmacokinetics of antisense oligonucleotide drugs / Arthur A. Levin, Rosie Z. Yu, and Richard S. Geary -- Routes and formulations for delivery of antisense oligonucleotides / Gregory E. Hardee, Lloyd G. Tillman, and Richard S. Geary -- Liposomal formulations for nucleic acid delivery / Ian MacLachlan -- Pharmacological properties of 2'-O-methoxyethyl-modified oligonucleotides / C. Frank Bennett -- Pharmacokinetic/pharmacodynamic properties of phosphorothioate 2'-O-(2-methoxyethyl)-modified antisense oligonucleotides in animals and man / Richard S. Geary ... [et al.] -- Toxicologic properties of 2'-O-methoxyethyl chimeric antisense inhibitors in animals and man / Scott P. Henry ... [et al.] -- An overview of the clinical safety experience of first- and second-generation antisense oligonucleotides / T. Jesse Kwoh -- Manufacturing and analytical processes for 2'-O-(2-methoxyethyl)-modified oligonucleotides / Daniel C. Capaldi and Anthony N. Scozzari -- Utilizing chemistry to harness RNA interference pathways for therapeutics : chemically modified siRNAs and antagomirs / Muthiah Manoharan and Kallanthottathil G. Rajeev. Discovery and development of RNAi therapeutics / Antonin R. deFougerolles and John M. Maraganore -- Optimization of second-generation antisense drugs: going beyond generation 2.0 / Brett P. Monia ... [et al.] -- Modulating gene function with peptide nucleic acids (PNA) / Peter E. Nielsen -- Locked nucleic acid / Troels Koch and Henrik Ørum -- Morpholinos / Patrick L. Iversen -- Potential therapeutic applications of antisense oligonucleotides in ophthalmology / Lisa R. Grillone and Scott P. Henry -- Cardiovascular therapeutic applications / Rosanne M. Crooke, Brenda Baker, and Mark Wedel -- Developing antisense drugs for metabolic diseases: a novel therapeutic approach / Sanjay Bhanot -- Inflammatory diseases / Susan A. Gregory and James G. Karras -- Antisense oligonucleotides for the treatment of cancer / Boris A. Hadaschik and Martin E. Gleave -- Targeting neurological disorders with antisense oligonucleotides / Richard A. Smith and Timothy M. Miller -- Mechanisms and therapeutic applications of immune modulatory oligodeoxynucleotide and oligoribonucleotide ligands for toll-like receptors / Jörg Vollmer and Arthur M. Krieg -- Aptamer opportunities and challenges / Charles Wilson.
  • 2006 Springer Protocols
    edited by Julio Salinas and Jose J. Sanchez-Serrano.
    Applications of chemical-inducible expression systems in functional genomics and biotechnology / Jianru Zuo, Peter D. Hare, and Nam H. Chua -- RNA extraction / Huazhong Shi and Ray Bressan -- Target preparation for DNA microarray hybridization / Tong Zhu, Sherman H. Chang, and Pedro Gil -- Statistical issues in microarray data analysis / Willem A. Rensink and Samuel Hazen -- Preparation and quality assessment of rna from cell-specific samples obtained by laser microdissection / Regine Kleber and Julia Kehr -- Protein isolation and 2-D electrophroetic separation / Bernhard Schlesier and Hans-Peter Mock -- Isolation of nuclear proteins / Tomasz T. Calikowski and Iris Meier -- Puification and fractionation of membranes for proteomic analysis / Anne Marmagne ... [et al.] -- Protein microsequencing / Alberto Paradela and Juan Pablo Albar -- Peptide-based phosphoproteomics with immobilized metal ion chromatography / Thomas S. Nohse and Scott C. Peck -- Metabolite profiling in arabidopsis / Oliver Fiehn -- Hormone profiling in arabidopsis / Axel Müller, Petra Düchting, and Elmer W. Weiler. EMS mutagenesis of arabidopsis / YongSig Kim, Karen S. Schumaker, and Jian-Kang Zhu -- Low-resolution mapping of untagged mutations / M. Rosa Ponce ... [et al.] -- Gene identification and cloning by molecular marker mapping / Georg Jander -- High-throughput TILLING for arabidopsis / Bradley J. Till ... [et al.] -- Genotyping and mapping with high-density oligonucleotides arrays / Justin Borewitz -- Forward genetic screening of insertional mutants / Anja Schneider and Dario Leister -- PCR-based screening for insertional mutants / Anna N. Stepanova and Jose M. Alonso -- Cytogenetic analysis of arabidopsis / Martin Lysak, Paul Fransz, and Ingo Schubert -- Using information from public arabidopsis databases to aid research / Margarita García-Hernandez and Leonore Reiser -- In planta agrobacterium-mediated transformation by vacuum infiltration / Brian W. Tague and Joanna Mantis -- Transient expression assay by agroinfiltration of leaves / Min Woo Lee and Yinong Yang -- Runctional analysis of transcription factors by microparticle bombardment / Ida Ruberti, Giovanna Sessa, and Giorgio Morelli -- Transfection assays with protoplasts containing integrated reporter genes / Shiv Tiwari ... [et al.] -- Chloroplast genetic engineering via organogenesis or somatic embryogenesis / Amit Dhingra and Henry Daniell -- [beta]Glucuronidase as reporter gene: advantages and limitations / Kye-Won Kim ... [et al.] -- Use of fluorescent proteins as reporters / Guido Jach -- Luciferase as reporter genes / Megan M. Southern, Paul E. Brown, and Anthony Hall -- Aqueorin as reporter gene / Christoph. Plieth -- Growth of plants and preservation of seeds / Luz Rivero-Lepinckas, Deborah Crist, and Randy Scholl -- Prevention and control of pests and diseases / Jenifer Bush, Georg Jander, and Frederick M. Ausubel -- Growth of other species related to Arabidopsis thaliana / Krithika Yogeeswaran and Mikhail Elia Nasrallah -- Grafting / Katherine Bainbridge ... [et al.] -- Synchronization, transformation, and cryopreservation of suspension-cultured cells -- Margit Menges and James A. H. Murray -- Genetic analysis / Maarten Koorneeff, Carlos Alonso-Blanco, and Piet Stam -- QTL analysis / Carlos Alonso-Blanco, Maarten Koornneef, and Johan W. van Ooijen --
  • 2014 Springer Protocols
    edited by Jose J. Sanchez-Serrano, Julio Salinas.
    1. Handling Arabidopsis plants: growth, preservation of seeds, transformation and genetic crosses / Luz Rivero, Randy Scholl, Nicholas Holomuzki, Deborah Crist, Erich Grotewold, and Jelena Brkljacic --2. Using Arabidopsis related model species (ARMS): growth, genetic transformation and comparative gnomics / Giorgia Batelli, Dong-Ha Oh, Matilde Paino D'Urzo, Francesco Orsini, Maheshi Dassanayake, Jian-Kang Zhu, Hans J. Bohnert, Ray A. Bressan and Albino Maggio -- 3. Growing Arabidopsis in vitro: cell suspensions, in vitro culture, and regeneration / Bronwyn J. Barkla, Rosario Vera-Estrella, and Omar Pantoja -- 4. Arabidopsis database and stock resources / Donghui Li, Kate Dreher, Emma Knee, Jelena Brkljacic, Erich Grotewold, Tanya Z. Berardini, Philippe Lamesch, Margarita Garcia-Hernandez, Leonore Reiser and Eva Huala -- 5. Bioinformatic tools in Arabidopsis research / Miguel de Lucas, Nicholas J. Provart, and Siobhan Brady -- 6. Exploiting natural variation in Arabidopsis / Johanna A. Molenaar and Joost J. B. Keurentjes -- 7. Grafting in Arabidopsis / Katherine Bainbridge, Tom Bennett, Peter Crisp, Ottoline Leyser, and Colin Turnbull -- 8. Agrobacterium tumefaciens mediated transient transformation of Arabidopsis thaliana leaves / Silvina Mangano, Cintia Daniela Gonzalez, and Silvana Petruccelli -- 9. iTilling: personalized mutation screening / Susan M. Bush and Patrick J. Krysan --10. Tailor Made Mutations in Arabidopsis Using Zinc-Finger Nucleases / Yiping Qi, Colby G. Starker, Feng Zhang, Nicholas J. Baltes, and Daniel F. Voytas -- 11. The Use of Artificial Micro RNA Technology to Control Gene Expression in Arabidopsis thaliana / Andrew L. Eamens, Marcus McHale, and Peter M. Waterhouse -- 12. Generation and Identification of Arabidopsis EMS Mutants / Li-Jia Qu and Gengi Qin --13. Generation and Identification of Arabidopsis T-DNA Insertion Mutants / Li-Jia Qu and Gengi Qin --14. Identification of EMS-induced Causal Mutations in Arabidopsis thaliana by Next-Generation Sequencing /Naoyuki Uchida, Tomoaki Sakamoto, Masao Tasaka, and Tetsuya Kurata -- 15. Arabidopsis Transformation with Large Bacterial Artificial Chromosomes /Jose M. Alonso and Anna N. Stepanova --16. Global DNA Methylation Analysis Using Methyl-Sensitive Amplification Polymorphism (MSAP) / Mahmoud W. Yaish, Mingsheng Peng, and Steven J. Rothstein --17. Next Generation Mapping of Genetic Mutations Using Bulk Population Sequencing / Ryan S. Austin, Steven P. Chatfield, Darrell Desveaux, and David S. Guttman --18. Chemical Fingerprinting of Arabidopsis Using Fourier-transform Infrared (FT-IR) Spectroscopic Approaches /András Gorzsás and Björn Sundberg -- 19. A Pipeline for ¹⁵N Metabolic Labeling and Phosphoproteome Analysis in Arabidopsis thaliana / Benjamin B. Minkoff, Heather L. Burch, and Michael R. Sussman -- 20. Gene Expression Profiling Using DNA Microarrays / Kyonoshin Maruyama, Kazuko Yamaguchi-Shinozaki, and Kazuo Shinozaki -- 21. Forward Chemical Genetic Screening /Hyunmo Choi, Jun-Young Kim, Young Tae Chang, and Hong Gil Nam -- 22. Highly-Reproducible ChIP-On-Chip Analysis to Identify Genome-Wide Protein Binding and Chromatin Status in Arabidopsis thaliana / Jong-Myong Kim, Taiko Kim To, Maho Tanaka, Takaho A. Endo, Akihiro Matsui, Junko Ishida, Fiona C. Robertson, Tetsuro Toyoda, and Motoaki Seki -- 23. Fluorescence Microscopy / Sebastien Peter, Klaus Harter, and Frank Schleifenbaum -- 24. Immunocytochemical Fluorescent In Situ Visualization of Proteins in Arabidopsis / Yohann Boutté and Markus Grebe --25. High Pressure Freezing and Freeze Substitution of Arabidopsis For Electron Microscopy / Jotham R. Austin, II. -- 26. Applications of Fluorescent Marker Proteins in Plant Cell Biology / Michael R. Blatt and Christopher Grefen -- 27. Flow Cytometry and Sorting in Arabidopsis / David W. Galbraith -- 28. Live Imaging of Arabidopsis Development / Daniel von Wangenheim, Gabor Daum, Jan U. Lohmann , Ernst K. Stelzer, and Alexis Maizel -- 29. Arabidopsis Organelle Isolation and Characterisation / Nicolas L. Taylor, Elke Ströher, and A. Harvey Millar -- 30. Analysis of Subcellular Metabolite Distributions within Arabidopsis thaliana Leaf Tissue: A Primer for Subcellular Metabolomics / Stephan Krueger, Dirk Steinhauser, Jan Lisec, and Patrick Giavalisco -- 31. Hormone Profiling --Gaetan Glauser, Armelle Vallat, and Dirk Balmer -- 32. Purification of Protein Complexes and Characterization of Protein-Protein Interactions / Kirby N. Swatek, Chris B. Lee, and Jay J. Thelen -- 33. Protein Fragment Bimolecular Fluorescence Complementation Analysis for the In vivo Study of Protein-Protein Interactions and Cellular Protein Complex Localizations / Rainer Waadt, Kathrin Schlückling, Julian I. Schroeder, and Jörg Kudla -- 34. The Split Ubiquitin System for the Analysis of Three-Component Interactions Christopher Grefen -- 35. RNA-Binding Protein Immunoprecipitation from Whole Cell Extracts / Tino Köster and Dorothee Staiger -- 36. High Throughput Analysis of Protein-DNA Binding Affinity / José M. Franco-Zorrilla and Roberto Solano.
  • 2014 Springer Protocols
    edited by Shunichi Shiozawa.
    Intravital Multiphoton Microscopy for Dissecting Cellular Dynamics in Arthritic Inflammation and Bone Destruction -- Monitoring Multifunctionality of Immune-Exhausted CD8 T in Cancer Patients -- Characterization of Innate Immune Signalings Stimulated by Ligands for Pattern Recognition Receptors -- Principled for the Use of in vivo Transgene Techniques: Overview and an Introductory Practical Guide for the Selection of Tetracycline-Controlled Transgenic Mice -- Unraveling Autoimmunity with the Adoptive Transfer of T Cells from TCR-Transgenic Mice -- In Vivo Cell Transfer Assay to Detect Autoreactive T Cell Subsets -- Characterization of microRNAs and Their Targets -- Studies on the T Cell Receptor (TCR) Revision of Autoantibody-Inducing CD4T (aiCD4 T) Cell -- Basic Techniques for Studies of iNKT Cells and MAIT Cells -- Induction of de novo Autoimmune Disease in Normal Mice Upon Repeated Immunization with Antigen -- Mouse Models of Experimental Dermal Fibrosis: The Bleomycin-Induced Dermal Fibrosis -- Screening for Novel Serum Biomarker for Monitoring Disease Activity in Rheumatoid Arthritis Using iTRAQ Technology Based Quantitative Proteomic Approach -- Genome-Wide Genetic Study in Autoimmune Disease Prone Mice -- Bayesian Systems-Based Genetic Association Analysis with Effect Strength Estimation and Omic Wide Interpretation: A Case Study in Rheumatoid Arthritis -- Sample Processing, Protocol and Statistical Analysis of the Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS) of Protein, Cell and Tissue Samples -- Label-Free Imaging of Adipogenesis by Coherent Anti-Stokes Raman Scattering Microscopy.
  • 2011 Springer Protocols
    edited by Pier Carlo Braga, Davide Ricci.
    How the atomic force microscope works / Bruno Torre, Davide Ricci, and Pier Carlo Braga -- Measurement methods in atomic force microscopy / Bruno Torre [and others] -- Recognizing and avoiding artifacts in atomic force microscopy imaging / Claudio Canale [and others] -- Imaging the spatial orientation of subunits within membrane receptors by atomic force microscopy / Stewart M. Carnally, J. Michael Edwardson, and Nelson P. Barrera -- High resolution imaging of immunoglobulin G antibodies and other biomolecules using amplitude modulation atomic force microscopy in air / Sergio Santos and Neil H. Thomson -- Atomic force microscopy of ex vivo amyloid fibrils / Claudio Canale, Annalisa Relini, and Alessandra Gliozzi -- Studying collagen self-assembly by time-lapse high-resolution atomic force microscopy / Clemens M. Franz and Daniel J. Muller -- Atomic force microscopy imaging of human metaphase chromosomes in liquid / Osamu Hoshi and Tatsuo Ushiki -- Atomic force microscopy of proteasome assemblies / Maria Gaczynska and Pawel A. Osmulski -- Atomic force microscopy of isolated mitochondria / Bradley E. Layton and M. Brent Boyd -- Imaging and interrogating native membrane proteins using the atomic force microscope / Andreas Engel -- Atomic force microscopy investigation of viruses / Alexander McPherson and Yurii G. Kuznetsov -- Determination of the kinetic on- and off-rate of single virus-cell interactions / Christian Rankl [and others] -- Atomic force microscopy as a tool for the study of the ultrastructure of trypanosomatid parasites / Wanderley de Souza [and others] -- Normal and pathological erythrocytes studied by atomic force microscopy / Andreas Ebner, Hermann Schillers, and Peter Hinterdorfer -- Growth cones of living neurons probed by the atomic force microscope / Davide Ricci, Massimo Grattarola, and Mariateresa Tedesco -- Highlights on ultrastructural pathology of human sperm / Narahari V. Joshi, Ibis Cruz, and Jesus A. Osuna -- High-speed atomic force microscopy and biomolecular processes / Takayuki Uchihashi and Toshio Ando -- Atomic force microscopy in mechanobiology : measuring microelastic heterogeneity of living cells / Evren U. Azeloglu and Kevin D. Costa -- Force-clamp measurements of receptor-ligand interactions / Felix Rico, Calvin Chu, and Vincent T. Moy -- Measuring cell adhesion forces : theory and principles / Martin Benoit and Christine Selhuber-Unkel -- Nanoscale investigation on E. coli adhesion to modified silicone surfaces / Ting Cao [and others] -- Imaging bacterial shape, surface, and appendages before and after treatment with antibiotics / Pier Carlo Braga and Davide Ricci -- Thymol-induced alterations in Candida albicans imaged by atomic force microscopy / Pier Carlo Braga and Davide Ricci -- Atomic force microscope-enabled studies of integrin-extracellular matrix interactions in vascular smooth muscle and endothelial cells / Zhe Sun and Gerald A. Meininger -- Atomic force microscopy studies on circular DNA structural changes by vincristine and aspirin / Zhongdang Xiao [and others] -- Combined atomic force microscopy and fluorescence microscopy / Miklos S.Z. Kellermayer -- Chemical modifications of atomic force microscopy tips / Regis Barattin and Normand Voyer -- Atomic force microscopy as nanorobot / Ning Xi [and others].
  • 2008 Springer Protocols
    edited by Robert J. McMahon.
    Preparation of avidin conjugates / Rosaria P. Haugland and Mahesh K. Bhalgat -- Coupling of antibodies with biotin / Rosaria P. Haugland and Wendy W. You -- Optimization of detection and quantification of proteins on membranes in very high and very low abundance using avidin and strptavidin / Sara R. Zwart and Brandon J. Lewis -- Use of the avidin (Imino) biotin system as a general approach to affinity precipitation / Ruth Freitag and Frank Hilbrig -- Capturing biotinylated proteins and peptides by avidin functional affinity electrophoresis / Bao-Shiang Lee ... [et al.] -- Functionality screen of streptavidin mutants by non-denaturing SDS-PAGE using biotin-4-fluorescein / Nicolas Humbert and Thomas R. Ward -- Application of biotin-4-fluorescein in homogeneous fluorescence assays for avidin, streptavidin, and biotin or biotin derivatives / Andreas Ebner ... [et al.] -- quantitative recovery of biotinylated proteins from streptavidin-based affinity chromatography resins / Christoph Rösli ... [et al.] -- High-yield production and purification of recombinant T7-tag mature streptavidin in glucose-stressed E. coli / Nicolas Humbert ... [et al.] -- Endogenous biotin in rat brain : implications for false-positive results with avidin-biotin and streptavidin-biotin techniques / Bruce E. McKay, Michael L. Molineux, and Ray W. Turner -- Pseudo-immunolabelling with the avidin-biotin-peroxidase complex due to the presence of endogenous biotin in the retina / Willem Kamphuis and Jan Klooster -- Use of synthetic peptides for identifying biotinylation sites in human histones / Gabriela Camporeale ... [et al.] -- Detection and quantitation of the activity of DNA methyltransferases using a biotin/avidin microplate assay / Kirsten Liebert and Albert Jeltsch -- Anti-biotin antibodies offer superior organelle-specific labelling of mitochondria over avidin or streptavidin / Elisabeth D. Coene, Michael K. Shaw, and David J. Vaux -- Mammalian lectin as tool in glycochemistry and histochemistry with relevance for diagnostic procedure / Karel Smetana, Jr. and Sabine André -- A multiplex approach to isotyping antigen-specific antibodies using biotinylated antigen/streptavidin-phycoerythrin / Krista McCutcheon -- Biotin-protein bond : instability and structural modification to provide stability for in vivo applications / Donald M. Mock and Anna Bogusiewicz.
  • 2007
    James D. Watson.
    Status: Not Checked OutLane Catalog Record
    From a living legend--James D. Watson, who shared the Nobel Prize for revealing the structure of DNA--comes a personal account of the making of a scientist. Watson shares the less revolutionary secrets he has found to getting along and getting ahead in a competitive world. Each phase of his experience yields age-specific practical advice. A believer in the intellectual promise of youth, Watson offers pointers to beginning scientists about choosing the projects that will shape their careers, the supreme importance of collegiality, and dealing with competitors within the same institution, even one who is a former mentor. Later he addresses the role and needs of science at large universities. Scorning political correctness, this irreverent romp through Watson's life and learning is a guide to anyone plotting a career in science (or most anything else), addressed both to the next generation and those who are entrusted with their minds.--From publisher description.
  • 2011
    Brenda A. Wilson, Abigail A. Salyers, Dixie D. Whitt, Malcolm E. Winkler.
    The power of bacteria -- Skin and mucosa : the first lines of defense against bacterial infections -- The innate immune system : always on guard -- The second line of defense : antibodies and cytotoxic T cells -- The normal human microbiota -- Microbes and disease : establishing a connection -- Mechanisms of genetic modification and exchange : role in pathogen evolution -- Identification of virulence factors : measuring infectivity and virulence -- Identification of virulence factors : molecular approaches for bacterial factors -- Identification of virulence factors : molecular approaches for host factors -- Bacterial strategies for evading or surviving host defense systems -- Toxins and other toxic virulence factors -- Delivery of virulence factors -- Virulence regulation -- Antimicrobial compounds -- How bacteria become resistant to antibiotics -- Vaccination : an underappreciated component of the modern medical armamentarium -- The gram-positive opportunistic pathogens -- The gram-negative opportunistic pathogens and emerging pathogens -- Biosecurity : the changing roles of microbiologists in an age of bioterrorism and emerging diseases.
  • 2008 Springer Protocols
    edited by Frank R. DeLeo and Michael Otto.
    Isolation and characterization of lipopolysaccharides / Michael A. Apicella -- Proteomic analysis of proteins secreted by Streptococcus pyogenes / Michelle A. Chaussee, Emily J. McDowell, and Michael S. Chaussee -- Proteomic analysis to investigate regulatory networks in Staphylococcus aureus / Susanne Engelmann and Michael Hecker -- Microarray comparative genomic hybridization for the analysis of bacterial population genetics and evolution / Caitriona M. Guinane and J. Ross Fitzgerald -- Detection and inhibition of bacterial cell-cell communication / Scott A. Rice ... [et al.] -- A system for site-specific genetic manipulation of the relapsing fever spirochete Borrelia hermsii / James M. Battisti, Sandra J. Raffel, and Tom G. Schwan -- Transposon mutagenesis of the lyme disease agent Borrelia burgdorferi / Philip E. Stewart and Patricia A. Rosa -- The biofilm exopolysaccharide polysaccharide intercellular adhesin--a molecular and biochemical approach / Cuong Vuong and Michael Otto -- Analysis of Staphylococcus aureus gene expression during PMN phagocytosis / Jovanka M. Voyich, Dan E. Sturdevant, and Frank R. DeLeo -- Examining the vector-host-pathogen interface with quantitative molecular tools / Jason E. Comer, Ellen A. Lorange, and B. Joseph Hinnebusch -- Intracellular localization of Brucella abortus and Francisella tularensis in primary murine macrophages / Jean Celli -- Rate and extent of Helicobacter pylori phagocytosis / Lee-Ann H. Allen -- Culture, isolation, and labeling of Anaplasma phagocytophilum for subsequent infection of human neutrophils / Dori L. Borjesson -- Ultrastructural analysis of bacteria-host cell interactions / David W. Dorward -- Infection of human monocyte-derived macrophages with Coxiella burnetii / Jeffrey G. Shannon and Robert A. Heinzen -- Infection of epithelial cells with Salmonella enterica / Olivia Steele-Mortimer -- Determining the cellular targets of reactive oxygen species in Borrelia burgdorferi / Julie A. Boylan and Frank C. Gherardini -- Bioluminescent imaging of bacterial biofilm infections in vivo / Jagath L. Kadurugamuwa and Kevin P. Francis -- The cotton rat as a model for Staphylococcus aureus nasal colonization in humans : cotton rat S. aureus nasal colonization model / John F. Kokai-Kun -- A non-human primate model of acute group A Streptococcus pharyngitis / Paul Sumby, Anne H. Tart, and James M. Musser -- Target-based antimicrobial drug discovery / Lefa E. Alksne and Paul M. Dunman -- Sequence analysis of the variable number tandem repeat in Staphylococcus aureus protein A gene : spa typing / Barun Mathema, Jose Mediavilla, and Barry N. Kreiswirth.
  • 2008 Springer
    Walid El Sharoud, editor.
  • 2009 Springer
    edited by Philip T. Cagle and Timothy Craig Allen.
  • 2012
    Sergio Bacallado de Lara.
    A host of sequential models in probability and statistics are characterized by time reversibility, from Markov chain Monte Carlo samplers to queueing networks. In physics, this property arises naturally from Hamiltonian mechanics. Molecular dynamics simulations are computer experiments which approximate classical mechanics in a system of interacting particles; in consequence, they are frequently reversible. Recent technical progress has made it possible to investigate the dynamics of biological macromolecules in silico using molecular dynamics simulations. An active area of research within this field is concerned with modeling the output of a simulation stochastically. This dissertation deals with the problem of incorporating knowledge of reversibility into the estimation and testing of stochastic models. We define a range of Bayesian inference algorithms, which are motivated by specific problems in the analysis of molecular dynamics simulations.
  • 2015 Springer
    Abhijit Chakrabarti.
    Consists of critical reviews and original research papers from the 2014 International Symposium on the "Biochemical Role of Eukaryotic Cell Surface Macromolecules". Topics covered include: · neurochemical and biochemical analysis of cell surface glycoconjugates · membrane skeletal organization · GPCRs and other receptors · biophysical approaches to study membrane interactions · glycoconjugate metabolism · dysregulation · molecular mechanisms involved in cell-cell and cell-matrix interaction · glycans in infectious and neurological diseases · cancer and glycosyltransferases as drug targets.
    Also available: Print – 2015
  • 2012 Springer
    Perumana R. Sudhakaran, Avadhesha Surolia, editors.
    Glycomics: An Overview of the Complex Glycocode / Garima Gupta and Avadhesha Surolia -- Structural Glycomic Approaches to Molecular Recognition Events on Cell Surfaces / Koichi Kato -- ISCSM2011 Chondroitin Sulfate E-type Structure at Tumor Cell Surface Is Involved in Experimental Metastasis / Kazuyuki Sugahara and Shuji Mizumoto -- Chondroitin Sulfate-Specific Novel Hydrolase in Human / Shuhei Yamada -- Identification of Endothelial Cell Surface Carbohydrate-Binding Receptors by Carbohydrate Ligand Mimicry Peptides / Michiko N. Fukuda -- Lysosomal Enzyme Sorting Receptors - Where Did They First Appear in the Animal Kingdom? / Suryanarayanaraju Vegiraju, Suresh Koduru and Siva Kumar Nadimpalli -- Endoplasmic Reticulum-Targeted Bcl-2 Inhibitable Mitochondrial Fragmentation Initiates ER Stress-Induced Cell Death / B. C. Bhavya, Deepa Indira, Mahendra Seervi, Jeena Joseph and Praveen K. Sobhan, et al. -- Interactions Between Caveolin-1 and Sphingolipids, and Their Functional Relevance / Sandro Sonnino, Simona Prioni, Vanna Chigorno and Alessandro Prinetti -- Cell Membrane Repair Pathway Involves Sensing of Dynamics of Caveolae and Caspase-1 / Saumya S. Srivastava and M. V. Krishnasastry -- Angiogenic Response of Endothelial Cells to Fibronectin / V. B. S. Kumar, R. I. Viji, M. S. Kiran and Perumana R. Sudhakaran -- Lactosylceramide Synthase as a Therapeutic Target to Mitigate Multiple Human Diseases in Animal Models / Subroto Chatterjee and Nezar Alsaeedi -- Advanced FRET Methodologies: Protein-Lipid Selectivity Detection and Quantification / Fábio Fernandes, Manuel Prieto and Luís M. S. Loura -- Mechanism of GPCR-Directed Autoantibodies in Diseases / Hamiyet Unal, Rajaganapathi Jagannathan and Sadashiva S. Karnik -- Role of Membrane Cholesterol in Leishmanial Infection / Amitabha Chattopadhyay and Md. Jafurulla -- How Intact Is the Basement Membrane? Role of MMPs / S. Asha Nair, Sankar Jagadeeshan, Ramachandran Indu, Perumana R. Sudhakaran and M. R. Pillai -- Apoptosis of Breast Cancer Cells: Modulation of Genes for Glycoconjugate Biosynthesis and Targeted Drug Delivery / Subhash Basu, Rui Ma, Joseph R. Moskal, Manju Basu and Sipra Banerjee -- Altered Expression of Sialidases in Human Cancer / Taeko Miyagi, K. Takahashi, S. Moriya, K. Hata and K. Yamamoto, et al. -- Poly-ADP-Ribosylation of Vascular Endothelial Growth Factor and Its Implications on Angiogenesis / S. Binu, S. J. Soumya, V. B. S. Kumar and Perumana R. Sudhakaran -- Sphingolipid-Binding Domain in the Serotonin1A Receptor / Amitabha Chattopadhyay, Yamuna Devi Paila, Sandeep Shrivastava, Shrish Tiwari and Pushpendra Singh, et al. -- Withanolide D, Carrying the Baton of Indian Rasayana Herb as a Lead Candidate of Antileukemic Agent in Modern Medicine / Susmita Mondal, Saptarshi Roy, Rita Maity, Asish Mallick and Rajender Sangwan, et al. -- Engineered Glucose to Generate a Spectroscopic Probe for Studying Carbohydrate Biology / Ashish Tripathi, Vibha Singh, K. G. Aishwarya, Gopala Krishna Aradhyam and Srinivas Hotha -- Changes in Sialic Acid Content of Jelly Coat in Pesticide-Exposed Frog Eggs and Their Influence on Fertilization / H. P. Gurushankara, S. V. Krishnamurthy and V. Vasudev.
    Also available: Print – 2012
  • 2007 CRCnetBASE
    Roger L. Lundblad.
    Abbreviations and acronyms -- Glossary of terms useful in biochemistry and molecular biology and related disciplines -- Chemicals commonly used in biochemistry and molecular biology and their properties -- A listing of log P values, water solubility, and molecular weight for some selected chemicals -- Protease inhibitors and protease inhibitor cocktails -- List of buffers -- Organic name reactions useful in biochemistry and molecular biology.
  • 2002 CRCnetBASE
    edited by M.A. Vijayalakshmi.
    Upstream and downstream steps in biochromatography / Mookambeswaran A. Vijayalakshmi -- Gel filtration / Kjell-Ove Eriksson -- Ion exchange interaction biochromatography / E. Boschetti and P. Girot -- Hydrophobic (interaction) chromatography of proteins / Herbert P. Jennissen -- Conformational behaviour of polypeptides and proteins in reversed phase and lipophilic environments / Milton W. Hearn -- Affinity chromatography / Jaroslava Turková -- Dye ligand affinity chromatography / Robert K. Scopes -- Immobilized synthetic dyes in affinity chromatography / Nikos E. Labrou and Yannis D. Clonis -- Immobilized histidine ligand affinity chromatography / Oliver Pitiot and Mookambeswaran A. Vijayalakshmi -- Immobilized metal-ion affinity chromatography: from phenomenological hallmarks to structure-based molecular insights / Nadir T. Mrabet and Mookambeswaran A. Vijayalakshmi -- Thiophilic interaction chromatography / Sven Oscarsson and Francisco Batista Viera -- Miscellaneous methods in affinity chromatography: Part 1: Boronic acids as selective ligands for affinity chromatography / Xio-Chuan Liu and William H. Scouten -- Miscellaneous methods in affinity chromatography: Part 2: Shielded affinity chromatography in packed bed and expanded bed mode / Igor Yu. Galaev and Bo Mattiasson -- Glycobiology and biochromatography / Henri Debray, Gérard Strecker and Jean Montreuil -- Capillary electrokinetic chromatography / Gargi Choudhary and Csaba Horváth -- Imprinted polymers as tailor-made stationary phases for affinity separation / Karsten Haupt, Peter A.G. Cormack and Klaus Mosbach -- Computer-aided simulation of biochromatography / Nicolas Voute -- Industrial biochromatography: engineering aspects / F.A.P. Garcia, D.M.F. Prazeres and J.M.S. Cabral -- Validation aspects in biochromatography / Charles Lutsch -- Biochromatography and biomedical applications / C. Legallais, Mookambeswaran A. Vijayalakshmi and Ph. Moriniére.
  • 2006
    by Jean-Michel Claverie and Cedric Notredame.
    Status: Not Checked OutLane Catalog Record
    Introduction -- PART I: GETTING STARTED IN BIOINFORMATICS: Finding out what bioinformatics can do for you -- How most people use bioinformatics -- PART II: A SURVIVAL GUIDE TO BIOINFORMATICS: Using nucleotide sequence databases -- Using protein and specialized sequence databases -- Working with a single DNA sequence -- Working with a single protein sequence -- PART III: BECOMING A PRO IN SEQUENCE ANALYSIS: Similarity searches on sequence databases -- Comparing two sequences -- Building a multiple sequence alignment -- Editing and publishing alignments -- PART IV: BECOMING A SPECIALIST: ADVANCED BIOINFORMATICS TECHNIQUES: Working with protein 3-D structures -- Working with RNA -- Building phytogenetic trees -- PART V: THE PART OF TENS: The ten (okay, twelve) commandments for using servers -- Some useful bioinformatics resources.
  • 2006
    Robert G. McKinnell, Ralph E. Parchment, Alan O. Perantoni, Ivan Damjanov, G. Barry Pierce.
    Status: Not Checked OutLane Catalog Record
    The pathology of cancer / G. Barry Pierce and Ivan Damjanov -- Invasion and metastasis / Robert G. McKinnell -- Carcinogenesis / Alan O. Perantoni -- Genetics and heredity / Robert G. McKinnell -- Cancer-associated genes / Alan O. Perantoni -- Cancer in nonhuman organisms / Robert G. McKinnell -- Epidemiology / Robert G. McKinnell -- Lifestyle: is there anything more important? / Robert G. McKinnell -- The stem cell basis of cancer treatment: concepts and clinical outcomes / Ralph E. Parchment -- Oncology: the difficult task of eradicating caricatures of normal tissue renewal in the human patient / Ralph E. Parchment.
  • 2010 Springer Protocols
    edited by Ali Khademhosseini, Kahp-Yang Suh, Mohammed Zourob.
    RNA and DNA microarrays / S.C. Sealfon and T.T. Chu -- Aptamer arrays / E. Baldrich -- Oligonucleotide microarrays for identification of microbial pathogens and detection of their virulence-associated or drug-resistance determinants / D.V. Volokhov ... [et al.] -- Protein microarrays printed from DNA microarrays / O. Stoevesandt, M. He and M.J. Taussig -- Lithographically defined two- and three-dimensional tissue microarrays / E.W. Gomez and C.M. Nelson -- Ratiometric lectin microarray analysis of the mammalian cell surface glycome / K.L. Hsu ... [et al.] -- Cell microarrays based on hydrogel microstructures for the application to cell-based biosensor / W.G. Koh -- Fabrication of bacteria and virus microarrays based on polymeric capillary force lithography / P.J. Yoo -- 3D polymer scaffold arrays / C.G. Simon, Jr. ... [et al.] -- PDMS microfluidic capillary systems for patterning proteins on surfaces and performing miniaturized immunoassays / M. Pla-Roca and D. Juncker -- Merging photolithography and robotic protein printing to create cellular microarrays / J.Y. Lee and A. Revzin -- Generation of protein and cell microarrays on functionalized surfaces / Y.S. Choi and C.S. Lee -- Microprinting of liver micro-organ for drug metabolism study / R.C. Chang ... [et al.] -- Microcontact printing / Y. Xie and X. Jiang -- Micromolding for the fabrication of biological microarrays / A.L. Galloway ... [et al.] -- Progress report on microstructured surfaces based on chemical vapor deposition / Y. Elkasabi and J. Lahann -- Methods for forming human microvascular tubes in vitro and measuring their macromolecular permeability / G.M. Price and J. Tien -- Microarray bioinformatics / R.P. Loewe and P.J. Nelson
  • 2002 Wiley
    Steen Knudsen.
    Preface xi -- Acknowledgments xiii -- 1 Introduction I -- 1.1 Hybridization 1 -- 1.2 Affymetrix GeneChip Technology 3 -- 1.3 Spotted Arrays 6 -- 1.4 Serial Analysis of Gene Expression (SAGE) 8 -- 1.5 Example: Affymetrix vs. Spotted Arrays 9 -- 1.6 Summary 11 -- 1.7 Further Reading 13 -- 2 Overview of Data Analysis 15 -- 3 Basic Data Analysis 17 -- 3.1 Absolute Measurements 17 -- 3.2 Scaling 18 -- 3.2.1 Example: Linear and Nonlinear Scaling 20 -- 3.3 Detection of Outliers 20 -- 3.4 Fold Change 21 -- 3.5 Significance 22 -- 3.5.1 Nonparametric Tests 24 -- 3.5.2 Correction for Multiple Testing 24 -- 3.5.3 Example I: t-Test and ANOVA 25 -- 3.5.4 Example II: Number of Replicates 26 -- 3.6 Summary 28 -- 3.7 Further Reading 29 -- 4 Visualization by Reduction of Dimensionality 33 -- 4.1 Principal Component Analysis 33 -- 4.2 Example 1: PCA on Small Data Matrix 35 -- 4.3 Example 2: PCA on Real Data 37 -- 4.4 Summary 37 -- 4.5 Further Reading 39 -- 5 Cluster Analysis 41 -- 5.1 Hierarchical Clustering 41 -- 5.2 K-means Clustering 43 -- 5.3 Self-Organizing Maps 44 -- 5.4 Distance Measures 45 -- 5.4.1 Example: Comparison of Distance Measures 47 -- 5.5 Normalization 49 -- 5.6 Visualization of Clusters 50 -- 5.6.1 Example: Visualization of Gene Clusters in -- Bladder Cancer 50 -- 5.7 Summary 50 -- 5.8 Further Reading 52 -- 6 Beyond Cluster Analysis 55 -- 6.1 Function Prediction 55 -- 6.2 Discovery of Regulatory Elements in Promoter -- Regions 56 -- 6.2.1 Example 1: Discovery of Proteasomal Element 57 -- 6.2.2 Example 2: Rediscovery of Mlu Cell Cycle -- Box (MCB) 57 -- 6.3 Integration of data 58 -- 6.4 Summary 59 -- 6.5 Further Reading 59 -- 7 Reverse Engineering of Regulatory Networks 63 -- 7.1 The Time-Series Approach 63 -- 7.2 The Steady-State Approach 64 -- 7.3 Limitations of Network Modeling 65 -- 7.4 Example 1: Steady-State Model 65 -- 7.5 Example 2: Steady-State Model on Real Data 66 -- 7.6 Example 3: Steady-State Model on Real Data 68 -- 7.7 Example 4: Linear Time-Series Model 68 -- 7.8 Further Reading 71 -- 8 Molecular Classifiers 75 -- 8.1 Classification Schemes 76 -- 8.1.1 Nearest Neighbor 76 -- 8.1.2 Neural Networks 76 -- 8.1.3 Support Vector Machine 76 -- 8.2 Example I: Classification of Cancer Subtypes 77 -- 8.3 Example II: Classification of Cancer Subtypes 78 -- 8.4 Summary 79 -- 8.5 Further Reading 79 -- 9 Selection of Genes for Spotting on Arrays 81 -- 9.1 Gene Finding 82 -- 9.2 Selection of Regions Within Genes 82 -- 9.3 Selection of Primers for PCR 83 -- 9.4 Selection of Unique Oligomer Probes 83 -- 9.4.1 Example: Finding PCR Primers for Gene -- AF105374 83 -- 9.5 Experimental Design 84 -- 9.6 Further Reading 84 -- 10 Limitations of Expression Analysis 87 -- 10.1 Relative VersusAbsoluteRNA Quantification 88 -- 10.2 Further Reading 88 -- 11 Genotyping Chips 91 -- 11.1 Example: NeuralNetworksfor GeneChipprediction 91 -- 11.2 Further Reading 93 -- 12 Software Issues and Data Formats 95 -- 12.1 Standardization Efforts 96 -- 12.2 Standard File Format 97 -- 12.2.1 Example: Small Scripts in Awk 97 -- 12.3 Software for Clustering 98 -- 12.3.1 Example: Clustering with ClustArray 99 -- 12.4 Software for Statistical Analysis 99 -- 12.4.1 Example: StatisticalAnalysis with R 99 -- 12.4.2 The affyR Software Package 103 -- 12.4.3 Commercial Statistics Packages 103 -- 12.5 Summary 103 -- 12.6 Further Reading 104 -- 13 Commercial Software Packages 105 -- 14 Bibliography 109 -- Index 123.
  • 2013 Springer
    edited by C. Yan Cheng.
    Regulation of permeability across the blood-brain barrier / Alexander S. Easton -- Drug transporters at brain barriers : expression and regulation by neurological disorders / Tamima Ashraf ... [et al.] -- The blood-retina barrier : tight junctions and barrier modulation / Matthew Campbell and Peter Humphries -- The inner blood-retinal barrier : molecular structure and transport biology / Ken-Ichi Hosoya and Masanori Tachikawa -- Endothelial and epithelial barriers in graft-versus-host disease / Sam C. Nalle and Jerrold R. Turner -- Structure and regulation of intestinal epithelial tight junctions : current concepts and unanswered questions / Andrei I. Ivanov -- Polarity protein complex scribble/Lgl/Dlg and epithelial cell barriers / Wen-Hui Su ... [et al.] -- The blood-biliary barrier, tight junctions, and human liver diseases / Nikki P. Lee -- The blood-follicle barrier (BFB) in disease and in ovarian function / Michelle K.Y. Siu and C. Yan Cheng -- Physiology and pathophysiology of the epithelial barrier of the female reproductive tract : role of ion channels / Hsiao Chang Chan ... [et al.] -- The blood-epididymis barrier and human male fertility / Øvemie Dubø and Daniel G. Cyr -- Blood-tissue barriers : morphofunctional and immunological aspects of the blood-testis and blood-epididymal barriers / Luiz R. França ... [et al.] -- Gap junctions and blood-tissue barriers / Michelle W.M. Li, Dolores D. Mruk, and C. Yan Cheng -- Transcriptional regulation of cell adhesion at the blood-testis barrier and spermatogenesis in the testis / Wing-Yee Lui and C. Yan Cheng -- C-Src and c-Yes are two unlikely partners of spermatogenesis and their roles in blood-testis barrier dynamics / Xiang Xiao ... [et al.] -- Role of P-glycoprotein at the blood-testis barrier on adjudin distribution in the testis : a revisit of recent data / Linlin Su ... [et al.] -- The apical ectoplasmic specialization-blood-testis barrier functional axis is a novel target for male contraception / Ka-Wai Mok ... [et al.].
    Also available: Print – 2013
  • 2009 Springer
    Ryozo Nagai, Scott L. Friedman, Masato Kasuga, editors.
    I. Overview. -- Krüppel-like Factors: Ingenious Three Fingers Directing Biology and Pathobiology / Ryozo Nagai, Ichiro Manabe and Toru Suzuki -- II. Molecular Control of Krüppel-like Factor Function. -- Molecular Structures of Krüppel-like Factors / Toshio Nagashima, Fumiaki Hayashi, Takashi Umehara and Shigeyuki Yokoyama -- Krüppel-like Factor Proteins and Chromatin Dynamics / Navtej S. Buttar, Gwen A. Lomberk, Gaurang S. Daftary and Raul A. Urrutia -- Co-regulator Interactions in Krüppel-like Factor Transcriptional Programs / Richard C. M. Pearson, Briony H. A. Jack, Stella H. Y. Lee, Alister P. W. Funnell and Merlin Crossley -- III. Krüppel-like Factors in Development and Differentiation. -- Developmental Expression of Krüppel-like Factors / Yizeng Yang and Jonathan P. Katz -- Expanded Role for EKLF/KLF1 Within the Hematopoietic Lineage / James J. Bieker -- Roles of Krüppel-like Factors in Lymphocytes / Kensuke Takada, Kristin A. Hogquist and Stephen C. Jameson -- Krüppel-like Factors in Gastrointestinal Tract Development and Differentiation / Marie-Pier Tétreault and Jonathan P. Katz -- Gene Interactions Between Krüppel-like Factors in Development / Joyce A. Lloyd -- Krüppel-like Factors in Stem Cell Biology / Masatsugu Ema, Satoru Takahashi and Yoshiaki Fujii-Kuriyama -- IV. Krüppel-like Factors in Organ Function and Disease. -- Krüppel-like Factors and the Liver / Goutham Narla and Scott L. Friedman -- Role of Krüppel-like Factor 15 in Adipocytes / Wataru Ogawa, Hiroshi Sakaue and Masato Kasuga -- Krüppel-like Factors in the Heart / Daiji Kawanami, Saptarsi M. Haldar and Mukesh K. Jain -- Krüppel-like Factors in the Vascular Endothelium / Guillermo García-Cardeña and Guadalupe Villarreal -- Krüppel-like Factors KLF2, KLF4, and KLF5: Central Regulators of Smooth Muscle Function / Christopher W. Moehle and Gary K. Owens -- Krüppel-like Factors in Cancers / Vincent W. Yang -- V. Diagnostic and Therapeutic Applications of Krüppel-like Factors. -- Krüppel-like Factors KLF6 and KLF6-SV1 in the Diagnosis and Treatment of Cancer / Analisa DiFeo, Goutham Narla and John A. Martignetti -- Drug Development and Krüppel-like Factors / Ichiro Manabe and Ryozo Nagai.
  • 2011 ScienceDirect
    edited by James C. Whisstock and Phillip I. Bird.
    Also available: Print – 2011
  • 2009 Springer
    Dov Zipori.
  • 2008. Springer
    Christoph W. Turck, editor.
  • 2012 Springer
    Jaroslav Šebestík, Milan Reiniš, Jan Ježek.
    Introduction -- Chemistry and structure of dendrimers. Definition of terms and nomenclature ; Sugar code (glyc code) ; Classes of peptide-, glyco, and glycopeptide dendrimers ; The dendritic state and dendritic effects ; Synthesis of dendrimers : convergent and divergent approaches ; Purification and characterization of dendrimers ; Dendrimeric libraries ; Dendrimers in catalysis -- Dendrimers and their biological and therapeutic applications. Dendrimers and solubility ; Biocompability and toxicity of dendrimers ; Dendrimers in nanoscience and nanotechnology ; Dendrimers in drug delivery ; Dendrimers in gene delivery ; Dendrimers and bacteria ; Dendrimers and viruses ; Dendrimers and parasites ; Cancer ; Diagnostics, lectin detection and cell-cell interactions ; Dendrimers as biosensors and imaging tools ; Dendrimers regulating in intracellular signaling pathways ; Vaccines and immunomodulation ; Dendrimers in neurodegenerative diseases ; Conclusions and perspectives. .
  • 2012 Springer Protocols
    edited by Luca Monticelli, Emppu Salonen.
    Ab Initio, density functional theory, and semi-empirical calculations / Mikael P. Johansson, Ville R.I. Kaila, and Dage Sundholm -- Ab Initio molecular dynamics / Kari Laasonen -- Introduction to QM/MM simulations / Gerrit Groenhof -- Computational enzymology / Alessio Lodola and Adrian J. Mulholland -- QM and QM/MM simulations of proteins / Thomas Steinbrecher and Marcus Elstner -- Classical molecular dynamics in a nutshell / Susanna Hug -- Enhanced sampling algorithms / Ayori Mitsutake, Yoshiharu Mori, and Yuko Okamoto -- Force fields for classical molecular dynamics / Luca Monticelli and D. Peter Tieleman -- Polarizable force fields / Hanne S. Antila and Emppu Salonen -- Electrostatics interactions in classical simulations / G. Andres Cisneros, Volodymyr Babin, and Celeste Sagui -- Introduction to best practices in free energy calculations / Michael R. Shirts and David L. Mobley -- Recipes for free energy calculations in biomolecular systems / Mahmoud Moradi [and others] -- Molecular docking methodologies / Andrea Bortolato [and others] -- Simulation studies of the mechanism of membrane transporters / Giray Enkavi [and others] -- Molecular dynamics simulations of lipid bilayers : simple recipe of how to do it / Hector Martinez-Seara and Tomasz Rog -- Simulations of lipid monolayers / Svetlana Baoukina and D. Peter Tieleman -- Simulating DNA by molecular dynamics : aims, methods, and validation / Nicolas Foloppe, Marc Gueroult, and Brigitte Hartmann -- Simulation of carbohydrates, from molecular docking to dynamics in water / Nicolas Sapay, Alessandra Nurisso, and Anne Imberty -- Systematic methods for structurally consistent coarse-grained models / W.G. Noid -- Martini coarse-grained force field / Xavier Periole and Siewert-Jan Marrink -- Multiscale molecular modeling / Matej Praprotnik and Luigi Delle Site -- Coarse-grained models for protein folding and aggregation / Philippe Derreumaux -- Elastic network models : theoretical and empirical foundations / Yves-Henri Sanejouand -- Introduction to dissipative particle dynamics / Zhong-Yuan Lu and Yong-Lei Wang -- Multiscale molecular dynamics simulations of membrane proteins / Syma Khalid and Peter J. Bond -- Vesicles and vesicle fusion : coarse-grained simulations / Julian C. Shillcock.
  • 2012 CRCnetBASE
    editor, V. K. Gupta ; co-editor, M. Ayyachamy.
    Identification of fungal pathogenicity genes by Agrobacterium tumefaciens-mediated transformation / Karunakaran Maruthachalam ... [et al.] -- The genetics and molecular biology of carotenoid biosynthesis in mucorales / Catalina Sanz, Mahdi Shahriari, and Arturo P. Eslava -- Induction and repression of carbohydrate degrading enzymes in fungi with special reference to their coding genes / Pauline Walsh, Alan Hernon, and Anthonia O'Donovan -- Involvement of ADH1, IPT1, and PMT genes in Candida albicans pathogenesis / Mahmoud Rouabhia and Mahmoud Ghannoum -- Enhanced resistance to fungal pathogens in plants through selective utilization of useful fungal genes / B.K. Sarma ... [et al.] -- Genes encoding penicillin and cephalosporin biosinthesis in Acremonium chrysogenum : two separate clusters are required for cephalosporin production / Juan-Francisco Martøn, Carlos Garcia-Estrada, and Ricardo V. Ullon -- A review of the fungal B-galactosidase gene and its biotechnological applications / Anne G. Lydon -- Trichoderma genes involved in interactions with fungi and plants / Mala Mukherjee -- Use of luciferases as a tool to analyze fungal physiology in association with gene transcription / David M. Arana and Jess Pla -- Fungal genes and their respective enzymes in industrial food, bio-based, and pharma applications / Marco van den Berg ... [et al.] -- Fungal metacaspases : an overview / Anthonia O'Donovan, Finola E. Cliffe, and Mary C. Shier -- Signaling molecules in the Arbuscular mycorrhizal fungi / Mohammad Miransari -- Role of pathogenic genes of Fusarium oxysporum, coding cell wall degrading enzymes during wilt infection in plants / Vijai Kumar Gupta, Maria Tuohy, and A. Manimaran -- Engineering fungal expression systems : recombinant expression of two class I alpha-1, 2-mannosidases from the filamentous fungus Aspergillus nidulans / C. Joshua Eades and William E. Hintz -- PCR-diagnostic of Septoria nodorum berk : aggresivenes in wheat tissue / I.V. Maksimov and A.Sh. Valeev -- Detection of toxigeneic and pathogenic fungi targeting metabolic pathway genes / Venkataramana Mudili ... [et al.] -- Pathogenicity genes in entomopathogenic fungia used as biopesticides / K. Uma Devi ... [et al.].
  • 2011 Springer Protocols
    edited by Sukriti Nag.
    Morphology and properties of brain endothelial cells -- Morphology and properties of pericytes -- Morphology and properties of astrocytes -- The blood-cerebrospinal fluid barrier: Structure and functional significance -- The blood-retinal barrier: Structure and functional significance -- The blood-nerve barrier: Structure and functional significance -- Detection of multiple proteins in intracerebral vessels by confocal microscopy -- Multiparametric magnetic resonance imaging and repeated measurements of blood-brain barrier permeability to contrast agents -- Detection of brain pathology by magnetic resonance imaging of iron oxide micro-particles -- Measuring the integrity of the human blood-brain barrier using magnetic resonance imaging -- Assessing blood-cerebrospinal fluid barrier permeability in the rat embryo -- Detection of blood-nerve barrier permeability by magnetic resonance imaging -- Isolation of human brain endothelial cells and characterization of lipid raft-associated proteins by mass spectroscopy -- Analysis of mouse brain microvascular endothelium using laser capture microdissection coupled with proteomics -- Molecular and functional characterization of P-glycoprotein in vitro -- Methods to study glycoproteins at the blood-brain barrier using mass spectrometry -- Novel models for studying the blood-brain and blood-eye barriers in drosophila -- Zebrafish model of the blood-brain barrier: Morphological and permeability studies -- Methods to assess pericyte-endothelial cell interactions in a coculture model -- Isolation and properties of an in vitro human outer blood-retinal barrier model -- Isolation and properties of endothelial cells forming the blood-nerve barrier -- Treatment of focal brain ischemia with viral vector-mediated gene transfer -- Blood-brain barrier disruption in the treatment of brain tumors -- Integrated platform for brain imaging and drug delivery across the blood-brain barrier -- Targeting the choroid plexus-CSF-brain nexus using peptides identified by phage display.
  • 2006 MyiLibrary
    [edited by] Martine J. Piccart ... [et al.].
  • 2015 Springer Protocols
    edited by David Biron, Gal Haspel.
    Library construction for mutation identification by whole-genome sequencing / Harold E. Smith -- Fundamentals of comparative genome analysis in caenorhabditis nematodes / Eric S. Haag and Cristel G. Thomas -- Genetic methods for cellular manipulations in C. elegans / Menachem Katz -- Fusion PCR method for expressing genetic tools in C. elegans / Yifat Eliezer and Alon Zaslaver -- Transposon-assisted genetic engineering with Mos1- mediated single-copy insertion (MosSCI) / Christian Frøkjær-Jensen -- Creating genome modifications in C. elegans using the CRISPR/Cas9 system / John A. Calarco and Ari E. Friedland -- Observing and quantifying fluorescent reporters / Michael Hendricks -- Microbial rhodopsin optogenetic tools : application for analyses of synaptic transmission and of neuronal network activity in behavior / Caspar Glock, Jatin Nagpal, and Alexander Gottschalk -- Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans / Nicholas F. Trojanowski and Christopher Fang-Yen -- High-pressure freeze and freeze substitution electron microscopy in C. elegans / Laura Manning and Janet Richmond -- Electron tomography methods for C. elegans / David H. Hall and William J. Rice -- Microfluidic devices for behavioral analysis, microscopy, and neuronal imaging in Caenorhabditis elegans / Ross C. Lagoy and Dirk R. Albrecht -- Tracking single C. elegans using a USB microscope on a motorized stage / Eviatar I. Yemini and André E. X. Brown -- Imaging system for C. elegans behavior / Matthew A. Churgin and Christopher Fang-Yen -- Method for obtaining large populations of synchronized Caenorhabditis elegans dauer larvae / Maria C. Ow and Sarah E. Hall -- Sampling and isolation of C. elegans from the natural habitat / Nausicaa Poullet and Christian Braendle -- Primer on prototyping / Dylan Lynch and David Biron -- Primer on quantitative modeling / Iulia Neagu and Erel Levine.
  • 2006 Springer Protocols
    edited by Kevin Strange.
    An overview of C. elegans biology / Kevin Strange -- Comparative genomics in C. elegans, C. briggsae, and other Caenorhabditis species / Avril Coghlan, Jason E. Stajich, and Todd W. Harris -- WormBase: methods for data mining and coparative genomics / Todd W. Harris and Lincoln D. Stein -- C. elegans deletion mutant screening / Robert J. Barstead and Donald G. Moerman -- Insertional mutagenesis in C. elegans using the drosophila transposon Mos1: a method for the rapid identification of mutated genes / Jean-Louis Bessereau -- Single nucleotide polymorphism mapping / M. Wayne Davis and Marc Hammarlund -- Creation of transgenic lines using microparticle bombardment methods / Vida Praitis -- Construction of plasmids for RNA interference and in vitro transcription of double-stranded RNA / Lisa Timmons -- Delivery methods for RNA interference in C. elegans / Lisa Timmons -- Functional genomic approaches in C. elegans / Todd Lamitina -- Assays for toxicity studies in C. elegans with Bt crystal proteins / Larry J. Bischof, Danielle L. Huffman, and Raffi V. Aroian -- Fluorescent reporter methods / Harald Hutter -- Electrophysiological analysis of neuronal and muscle function in C. elegans / Michael M. Francis and Andres Villu Maricq -- Sperm and oocyte isolation methods for biochemical and proteomic analysis / Michael A. Miller -- Preservation of C. elegans tissue via high-pressure freezing and freeze-substitution for ultrastructural analysis and immunocytochemistry / Robby M. Weimer -- Intracellular pH measurements in vivo using green fluorescent protein variants / Keith Nehrke -- Automated imaging of C. elegans behavior / Christopher J. Cronin, Zhaoyang Feng, and William R. Schafer -- Intracellular Ca2+ imaging in C. elegans / Rex A. Kerr and William R. Schafer -- In vitro culture of C. elegans somatic cells / Kevin Strange and Rebecca Morrison -- Techniques for analysis, sorting, and dispensing of C. elegans on the COPAS flow-sorting system / Rock Pulak.
  • ScienceDirect
    edited by Gerard Marriott.
    Also available: Print – 1998
  • 2010 ScienceDirect
    Frank H. Stephenson.
    Chapter 1. Scientific notation and metric prefixes -- Chapter 2. Solutions, mixtures, and media -- Chapter 3. Cell growth -- Chapter 4. Working with bacteriophages -- Chapter 5. Nucleic acid quantification -- Chapter 6. Labeling nucleic acids with radioisotopes -- Chapter 7. Oligonucleotide synthesis -- Chapter 8. The polymerase chain reaction (PCR) -- Chapter 9. The real-time polymerase chain reaction (RT-PCR) -- Chapter 10. Recombinant DNA -- Chapter 11. Protein -- Chapter 12. Centrifugation -- Chapter 13. Forensics and paternity.
  • 2014 CRCnetBASE
    edited by Kasirajan Ayyanathan, PhD.
  • 2015 Ovid
    editors, Vincent T. DeVita, Jr, Theodore S. Lawrence, Steven A. Rosenberg.
  • 2009 Springer Protocols
    edited by Ronald L. Cihlar and Richard A. Calderone.
    Isolation of dendritic cells from human blood for in vitro interaction studies with fungal antigens / T. Sreevalsan -- Detection and quantitation of antifungal SIgA antibodies in body fluids / Michael F. Cole -- Phagocytosis and killing assays for Candida species / Chen Du and Richard A. Calderone -- Immunization protocols for use in animal models of candidiasis / Esther Segal and Hana Sandovsky-Losica -- Penetration of antifungal agents through Candida biofilms / L. Julia Douglas -- Candida biofilm analysis in the artificial throat using FISH / Bastiaan P. Krom ... [et al.] -- Conditions for optimal Candida biofilm development in microtiter plates / Bastiaan P. Krom ... [et al.] -- Animal models of candidiasis / Cornelius J. Clancy, Shaoji Cheng, and Minh H. Nguyen -- Candida albicans gene expression in an in vivo infection model / Michael D. Kruppa -- In vitro and ex vivo assays of virulence in Candida albicans / Richard A. Calderone -- Biotyping of Candida albicans and other fungi by yeast killer toxins sensitivity / Luciano Polonelli and Stephania Conti -- DNA fingerprinting Candida species / Claude Pujol and David R. Soll -- The application of tandem-affinity purification to Candida albicans / Chris Blackwell and Jeremy D. Brown -- Preparation of samples for proteomic analysis of the Candida albicans cell wall / Neeraj Chauhan -- Reporter gene assays in Candida albicans / Joy Sturtevant -- Genetic transformation of Candida albicans / Ana M. Ramon and William A. Fonzi -- Large-scale gene disruption using the UAU1 cassette / Clarissa J. Nobile and Aaaron P. Mitchell -- Standard growth media and common techniques for use with Candida albicans / Neeraj Chauhan and Michael D. Kruppa.
  • 2008 Springer Protocols
    edited by Philippe Schmitt-Kopplin.
    Part I: Analyte-oriented -- Determination of small icons with capillary electrophoresis and contactless conductivity detection / Andreas Zemann, Irene Rohregger, and Roland Zitturi -- Metal analysis with capillary zone electrophoresis / Ashok Kumar Malik - Measurement of low-molecular-weight carboxylic acids in ambient air and vehicle emission by capillary electrophoresis / Ewa Dabek-Zlotorzynska and Valbona Celo -- Determination of aliphatic low-molecular-weight and biogenic amines by capillary zone electrophoresis / Agnes Fekete ... [et al.] -- Capillary electrophoretic analysis of organic pollutants / Ashok Kumar Malik, Jatinder Singh Aulakh, and Varinder Kaur -- Capillary electrophoresis with laser-induced fluorescence: environmental applications / Lee Riddick and William C. Brumley -- Practical considerations for the analysis of ionic and neutral organic molecules with capillary electrophoresis/mass spectrometry / Moritz Frommberger, Matthias Englmann, and Philippe Schmitt-Kopplin -- Analysis of the enantiomers of chiral pesticides and other pollutants in environmental samples by capillary electrophoresis / Arthur W. Garrison, Philippe Schmitt-Kopplin, and Jimmy K. Avants -- Capillary electrophoresis of tropane alkaloids and glycoalkaloids occurring in solanaceae plants / Tommaso R. I. Cataldi and Giuliana Bianco -- Capillary electrophoresis for pharmaceutical analysis / Alex Marsh ... [et al.] -- Capillary electrophoresis of neutral carbohydrates: mono-, oligosaccharides, and glucosides / Cristiana Campa and Marco Rossi -- Capillary electrophoresis of sugar acids / Cristiana Campa, Edi Baiutti, and Anna Flamigni -- Use of capillary electrophoresis for polysaccharide studies and applications / Amelia Gamini ... [et al.] -- Analysis of oligonucleotides using capillary zone electrophoresis and electrospray mass spectrometry / An Willems, Dieter L. Deforce, and Jan Van Bocxlaer -- Separation of DNA by capillary electrophoresis / Bruce McCord, Brittany Hartzell-Baguley, and Stephanie King -- Capillary electrophoresis of oxidative DNA damage / Guowang Xu ... [et al.] -- Capillary electrophoresis of gene mutation / Guowang Xu ... [et al.] -- Biomedical applications of amino acid detection by capillary electrophoresis / Giuseppe E. De Benedetto -- Separation of peptides by capillary electrophoresis / Gerhard K. E. Scriba and Arndt Psurek -- Analysis of proteins by capillary electrophoresis / Christian W. Huck and Günther K. Bonn -- Separation of synthetic (Co)polymers by capillary electrophoresis techniques / Hervé Cottet and Pierre Gareil -- Capillary electrophoresis separation of microorganisms / Bartolomé M. Simonet, Angel Ríos, and Miguel Valcárcel -- Part II: Methods-oriented -- A semi-empirical approach for a rapid comprehensive evaluation of the electrophoretic behaviors of small molecules in free-zone electrophoresis / Philippe Schmitt-Kopplin and Agnes Fekete -- The CE way of thinking: all is relative! / Philippe Schmitt-Kopplin and Agnes Fekete -- Adsorbed cationic polymer coatings for enhanced capillary electrophoresis/mass spectrometry of proteins / Sara Ullsten, Aida Zuberovic, and Jonas Bergquist -- On-column ligand/receptor derivatization coupled to affinity capillary electrophoresis / Jose Zavaleta ... [et al.] -- On-line concentration of environmental pollutant samples by using capillary electrophoresis / Janpen Intaraprasert and Philip J. Marriott -- Free-flow electrophoresis system for proteomics applications / Gerhard Weber and Robert Wildgruber -- Microemulsion electrokinetic chromatography / Wolfgang W. Buchberger -- Micellar electrokinetic chromatography of aminoglycosides / Ulrike Holzgrabe, Stefanie Laug, and Frank Wienen -- Capillary electrochromatography and on-line concentration / Guichen Ping ... [et al.] -- Analysis of alkaloids in single plant cells by capillary electrophoresis / Katrin Wieland, Björn Thiele, and Uli Schurr -- Multi-dimensional capillary electrophoresis and chromatography for proteomic analysis / Mingxia Gao and Xiangmin Zhang.
  • 2013 Springer Protocols
    edited by Nicola Volpi and Francesca Maccari.
    Recent advances in capillary electrophoresis-based proteomic techniques for biomarker discovery / Chenchen Wang, Xueping Fang, and Cheng S. Lee -- Capillary electrophoresis-mass spectrometry of carbohydrates / Joseph Zaia -- Approaches to enhancing the sensitivity of carbohydrate separations in capillary electrophoresis / M.C. Breadmore -- Determination of monosaccharides derivatized with 2-aminobenzoic acid by capillary electrophoresis / Mitsuru Abo, Li-ping He, Kae Sato and Akira Okubo -- Determination of mono-, di-, and oligosaccharides by capillary electrophoresis with capacitively coupled contactless conductivity detection / Claudimir Lucio do Lago, Thiago Nogueira, Lucas Blanes and Renata Mayumi Saito -- Separation of chitooligosaccharides in acidic solution by capillary electrophoresis / Toshiaki Hattori, Nobuhiro Anraku, and Ryo Kato -- Capillary electrophoresis for the analysis of glycosaminoglycan-derived disaccharides / Yuqing Chang, Bo Yang, Amanda Weyers and Robert J. Linhardt -- High-throughput capillary electrophoresis-mass spectrometry : from analysis of amino acids to analysis of protein complexes / Mehdi Moini -- Separation of amino acids by capillary electrophoresis with light-emitting diode-induced fluorescence in the presence of electroosmotic flow / Ming-Mu Hsieh and Po-Ling Chang -- Quantification of arginine and dimethylated arginines in human plasma by field-amplified sample injection capillary electrophoresis UV detection / Angelo Zinellu, Salvatore Sotgia, Luca Deianna and Ciriaco Carru -- Capillary electrophoresis-mass spectrometry for peptide analysis : target-based approaches and proteomics/peptidomics strategies / Carolina Simó, Alejandro Cifuentes, and Václav Kašička -- High resolution proteome/peptidome analysis of body fluids by capillary electrophoresis coupled with MS / Amaya Albalat, Vasiliki Bitsika, Petra Zurbig, Justyna Siwy and William Mullen -- Contribution of CE to the analysis of protein or peptide biomarkers / Kiarach Mesbah, Romain Verpillot, Franc̦ois de l'Escaille, Jean Bernard Falmagne and Myriam Taverna -- Highly charged polyelectrolyte coatings to prevent adsorption during protein and peptide analysis in capillary electrophoresis / Reine Nehmé and Catherine Perrin -- Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices : food and biological fluids / Raul Garrido-Medina, Angel Puerta, Cristina Pelaez-Lorenzo, Zuly Rivera-Monroy, Andras Guttman, Jose Carlos Diez-Masa and Mercedes de Frutos -- Automated capillary electrophoresis in the screening for hemoglobinopathies / Frédéric Cotton, Fleur Wolff, and Béatrice Gulbis -- Protein fingerprinting of Staphylococcus aureus by capillary electrophoresis with on-capillary derivatization and laser-induced fluorescence detection / Cristina Pelaez-Lorenzo, Maria Teresa Veledo, Ramon Gonzales, Mercedes de Frutos and Jose Carlos Diez-Masa -- Capillary electrophoresis of seed storage proteins : the separation and identification of microheterogeneous rice glutelin subunits / Tomoyuki Katsube-Tanaka -- Evaluating amyloid beta (A[beta]) 1-40 degradation by capillary electrophoresis / Benjamin J. Alper and Walter K. Schmidt -- Capillary electrophoresis for protein profiling of the dimorphic, pathogenic fungus, penicillium marneffei / Julie M. Chandler, Heather R. Trenary, Gary R. Walker and Chester R. Cooper -- Capillary electrophoretic enzyme assays / Gerhard K.E. Scriba, Hans Abromeit, Martina Hense and Yi Fan -- A methodology for detection and quantification of esterase activity / Ana L. Simplício, Ana S. Coroadinha, John F. Gilmer and Joana Lamego -- Enzyme inhibitor screening by CE with and on-column immobilized enzyme microreactor created by an ionic binding technique / Chao Liu, Qianqian Zhang, and Jingwu Kang -- Fluorescent lipids as probes for sphingosine kinase activity by capillary electrophoresis / Philip M. Yangyuoru, Latanya Hammonds-Odie and Simon M. Mwongela -- Assessment of DNA damage by micellar electrokinetic chromatography / Paolo Fattorini, Giorgio Marrubini, Pierangela Grignani, Solange Sorc̦aburu-Cigliero and Carlo Previderé.
  • 2012 Springer Protocols
    edited by Yann Chevolot.
    Recent advances and future challenges in glycan microarray technology / José L. de Paz and Peter H. Seeberger -- Chemical synthesis of carbohydrates and their surface immobilization : a brief introduction / Daniel B. Werz -- General consideration on sialic acid chemistry / Hongzhi Cao and Xi Chen -- Synthesis of azido-functionalized carbohydrates for the design of glycoconjugates / Sami Cecioni [and others] -- Polypyrrole-oligosaccharide microarray for the measurement of biomolecular interactions by surface plasmon resonance imaging / Julia Bartoli, André Roget, and Thierry Livache -- Glycosylated self-assembled monolayers for arrays and surface analysis / Fang Cheng and Daniel M. Ratner -- Carbohydrate microarrays for enzymatic reactions and quantification of binding affinities for glycan-protein interactions / Myung-Ryul Lee, Sungjin Park, and Injae Shin -- Neoglycolipid-based oligosaccharide microarray system : preparation of NGLs and their noncovalent immobilization on nitrocellulose-coated glass slides for microarray analyses / Yan Liu [and others] -- Preparation of a mannose-6-phosphate glycan microarray through fluorescent derivatization, phosphorylation, and immobilization of natural high-mannose N-glycans and application in ligand identification of P-type lectins / Xuezheng Song [and others] -- Production of fluorous-based microarrays with uncharged carbohydrates / Sahana K. Nagapayya and Nicola L.B. Pohl -- General procedure for the synthesis of neoglycoproteins and immobilization on epoxide-modified glass slides / Yalong Zhang and Jeffrey C. Gildersleeve -- Immobilization of polyacrylamide-based glycoconjugates on solid phase in immunosorbent assays / Oxana E. Galanina [and others] -- Surface plasmon resonance imaging analysis of protein binding to a sialoside-based carbohydrate microarray / Matthew J. Linman [and others] -- Glycoarray by DNA-directed immobilization / Franc̦ois Morvan [and others] -- Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides / Jonathan Popplewell [and others] -- Polysaccharide microarrays : application to the identification of heparan sulphate mimetics / Julien Dheur [and others] -- Carbohydrate antigen microarrays / Denong Wang -- Probing virus-glycan interactions using glycan microarrays / Jamie Heimburg-Molinaro [and others] -- MALDI-ToF MS analysis of glycosyltransferase activities on gold surface arrays / Nicolas Laurent [and others] -- Microarray technology using glycans extracted from natural sources for serum antibody fluorescent detection / Emanuela Lonardi [and others] -- Studying modification of aminoglycoside antibiotics by resistance-causing enzymes via microarray / Matthew D. Disney -- Microarray method for the rapid detection of glycosaminoglycan-protein interactions / Claude J. Rogers and Linda C. Hsieh-Wilson -- Neoglycolipid-based "designer" oligosaccharide microarrays to define [beta]-glucan ligands for Dectin-1 / Angelina S. Palma [and others] -- Measurement of antibodies to pneumococcal polysaccharides with luminex xMAP microsphere-based liquid arrays / Jerry W. Pickering and Harry R. Hill -- Carbohydrate microarrays in 96-well polystyrene microtiter plates / Jean-Philippe Ebran, Nabil Dendane, and Oleg Melnyk -- Photoimmobilization of saccharides / Gregory T. Carroll and Denong Wang -- Microwave-assisted method for fabrication of carbohydrate cluster microarrays on 3-dimensional hydrazide-dendrimer substrate / Xichun Zhou, Jian Zhang, and Denong Wang -- Combinatorial glycoarray / Simon Rinaldi, Kathryn M. Brennan, and Hugh J. Willison.
  • 2014 Springer Protocols
    edited by Milica Radisic, Lauren D. Black III.
    Second generation codon optimized minicircle (CoMiC) for nonviral reprogramming of human adult fibroblasts / Sebastian Diecke ... [and 3 others] -- Scalable cardiac differentiation of human pluripotent stem cells as microwell-generated, size controlled three-dimensional aggregates / Celine L. Bauwens and Mark D. Ungrin -- Preparation and characterization of circulating angiogenic cells for tissue engineering applications / Aleksandra Ostojic ... [and 4 others] -- Isolation and expansion of C-kit-positive cardiac progenitor cells by magnetic cell sorting / Kristin M. French and Michael E. Davis -- Synthesis of aliphatic polyester hydrogel for cardiac tissue engineering / Sanjiv Dhingra, Richard D. Weisel, and Ren-Ke Li -- Fabrication of PEGylated fibrinogen : a versatile injectable hydrogel biomaterial / Iris Mironi-Harpaz, Alexandra Berdichevski, and Dror Seliktar -- Natural cardiac extracellular matrix hydrogels for cultivation of human stem cell-derived cardiomyocytes / Donald O. Freytes ... [and 4 others] -- Magnetically actuated alginate scaffold : a novel platform for promoting tissue organization and vascularization / Yulia Sapir ... [and 3 others.] -- Shrink-induced biomimetic wrinkled substrates for functional cardiac cell alignment and culture / Nicole Mendoza ... [and 4 others] -- Injectable ECM scaffolds for cardiac repair / Todd D. Johnson, Rebecca L. Braden, and Karen L. Christman -- Generation of strip-format fibrin-based engineered heart tissue (EHT) / Sebastian Schaaf ... [and 5 others.] -- Cell tri-culture for cardiac vascularization / Ayelet Lesman, Lior Gepstein, and Shulamit Levenberg -- Cell sheet technology for cardiac tissue engineering / Yuji Haraguchi ... [and 8 others] -- Design and fabrication of biological wires / Jason W. Miklas ... [and 3 others] -- Collagen-based engineered heart muscle / Malte Tiburcy ... [and 3 others] -- Creation of a bioreactor for the application of variable amplitude mechanical stimulation of fibrin gel-based engineered cardiac tissue / Kathy Y. Morgan and Lauren D. Black III -- Preparation of acellular myocardial scaffolds with well- preserved cardiomyocyte lacunae, and method for applying mechanical and electrical simulation to tissue construct / Bo Wang ... [and 3 others] -- Patch-clamp technique in ESC-derived cardiomyocytes / Jie Liu and Peter H. Backx -- Optogenetic control of cardiomyocytes via viral delivery / Christina M. Ambrosi and Emilia Entcheva -- Methods for assessing the electromechanical integration of human pluripotent stem cell-derived cardiomyocyte grafts / Wei-Zhong Zhu ... [and 3 others] -- Quantifying electrical interactions between cardiomyocytes and other cells in micropatterned cell pairs / Hung Nguyen ... [and 4 others].
  • v. 1-2, 2006. Springer Protocols
    v. 2, 2006 Springer Protocols
    edited by Qing K. Wang.
    v. 1. Genetics -- v. 2. Molecular medicine.
  • 2014 Springer
    Christian Behl, Christine Ziegler.
    Aging and cell aging : an introduction -- Cell cycle : the life cycle of a cell -- Theories and mechanisms of aging -- Selected age-related disorders.
  • 2007 Springer
    Ralph Bock (ed.).
  • 2009 ClinicalKey
    edited by Raymond R. Tubbs, Mark H. Stoler.
    This volume in the Foundations in Diagnostic Pathology Series packs today's most essential cell and tissue base molecular pathology into a compact, high-yield format! It focuses on the state of the art in practical validated molecular diagnostics as applied across the fields of surgical pathology and cytology. With an emphasis on current, clinically valid, and diagnostically important applications today and in the near future, you can be assured you're getting the most up-to-date, authoritative coverage available. Its pragmatic, well-organized approach, nearly 250 full-color illustrations, and at-a-glance boxes and tables make the information you need easy to access. Practical and affordable, this resource is ideal for study and review as well as everyday clinical practice! Offers detailed discussions on today's technologies to help you select the best test for case evaluation. Presents recognized molecular pathologists who convey the most current information, keeping you on the cusp of your field. Features nearly 250 full-color illustrations that present important pathologic features, enabling you to form a differential diagnosis and compare your findings with actual cases. Uses a consistent, user-friendly format, including at-a-glance boxes and tables for easy reference.
  • 2014 Springer Protocols
    edited by Eishi Noguchi and Mariana C. Gadaleta.
    Cell cycle-regulated transcription : effectively using a genomics toolbox / Sara L. Bristow, Adam R. Leman, and Steven B. Haase -- Cell cycle regulation by checkpoints / Kevin J. Barnum and Matthew J. O'Connell -- Interplacy between the cell cycle and double-strand break response in mammalian cells / Kate Beishline and Jane Azizkhan-Clifford -- Cell cycle regulation by protein degradation / Deanna M. Koepp -- Linking chromosome duplication and segregation via sister chromatid cohesion / Adam R. Leman and Eishi Noguchi -- The greatwall : PP2A axis in cell cycle control / Peng Wang, Marcos Malumbres, and Vincent Archambault -- Cell cycle regulation by the nutrient-sensing mammalian target of rapamycin (mTOR) pathway / Elisabet Cuyàs ... [and 3 others] -- The senescence arrest program and the cell cycle / Alessandro Bitto ... [and 4 others] -- Oncogenic Ras pushes (and pulls) cell cycle progression through ERK activation / Paul M. Campbell -- Cell cycle regulation during viral infection / Sumedha Bagga and Michael J. Bouchard -- The roles of cohesins in mitosis, meiosis, and human health and disease / Amanda S. Brooker and Karen M. Berkowitz -- Introductory review of computational cell cycle modeling / Andres Kriete, Eishi Noguchi, and Christian Sell -- Cell cycle synchronization and flow cytometry analysis of mammalian cells / Naoko Yoshizawa-Sugata and Hisao Masai -- Analyzing transcription dynamics during the budding yeast cell cycle / Adam R. Leman, Sara L. Bristow, and Steven B. Haase -- Analyzing cell cycle checkpoints in response to ionizing radiation in mammalian cells / Bin Wang -- Analyzing DNA replication checkpoint in budding yeast / Nicole Hustedt and Kenji Shimada -- Analyzing cell cycle-dependent degradation and ubiquitination in budding yeast / Dong-Hwan Kim and Deanna M. Koepp -- Imaging analysis of cell cycle-dependent degradation of Cdt1 in mammalian cells Yasushi Shiomi ... [and 4 others] -- PCNA-dependent ubiquitination of Cdt1 and p21 in mammalian cells / Akiyo Hayashi ... [and 3 others] -- Analyzing Cdc2/Cdk1 activation during stress response in Schizosaccharomyces pombe / Miguel A. Rodríguez-Gabriel -- Analyzing Ras-associated cell proliferation signaling / Matthew C. Stout ... [and 4 others] -- Telomere regulation during the cell cycle in fission yeast / Bettina A. Moser, Ya-Ting Chang, and Toru M. Nakamura -- Detecting senescence : methods and approaches / Elizabeth P. Crowe ... [and 5 others] -- Analyzing RB and E2F during the G1-S transition / Michael J. Thwaites, Matthew J. Cecchini, and Frederick A. Dick -- Analyzing G1-S transcriptional control / Steffi Klier, Sarah Farmer, and Robertus A.M. de Bruin -- Analysis of replication timing using synchronized budding yeast cultures / Jie Peng, M.K. Raghuraman, and Wenyi Feng -- Analysis of ssDNA gaps and DSBs in genetically unstable yeast cultures / Jie Peng, M.K. Raghuraman, and Wenyi Feng -- Chromatin fractionation analysis of licensing factors in mammalian cells / Hideo Nishitani ... [and 4 others] -- Imaging analysis to determine chromatin binding of the licensing factor MCM2-7 in mammalian cells / Masayuki Morino ... [and 3 others] -- Chromatin immunopreciptiation to investigate origin association of replication factors in mammalian cells / Adam R. Leman and Eishi Noguchi -- Live-cell fluorescence imaging for phenotypic analysis of mitosis / Sushama Sivakumar, John R. Daum, and Gary J. Gorbsky -- Analyzing sister chromatid cohesion in mammalian cells / Katherine M. Feeney, Laura McFarlane-Majeed, and Joanna L. Parish -- Affinity purification of protein complexes from Drosophila embryos in cell cycle studies / Zoltan Lipinszki ... [and 8 others] -- Tracking histone variant nucleosomes across the human cell cycle using biophysical, biochemical, and cytological analyses / Marcin P. Walkiewicz ... [and 3 others].
  • 2014 Springer
    Hao Wu, editor.
    Beginning from centuries of anecdotal descriptions of cell death, such as those on the development of the midwife toad in 1842 by Carl Vogt, to modern-day investigations of cell death as a biological discipline, it has become accepted that cell death in multicellular organisms is a normal part of life. This book provides a comprehensive view of cell death, from its mechanisms of initiation and execution, to its implication in human disease and therapy. Physiological cell death plays critical roles in almost all aspects of biology, and the book details its roles in lymphocyte homeostasis, neuronal function, metabolism, and the DNA damage response.?When physiological cell death goes awry, diseases can arise, and cancer is presented as a central paradigm for the consequences of derangements in the interplay between cell survival and cell death. At the same time, the potential promise of targeted therapies aimed at interdicting cell death machineries are also discussed extensively. The molecular mechanisms that underlie apoptotic cell death are illustrated from the perspectives of both the intrinsic, mitochondrial apoptotic pathway and the extrinsic, death receptor pathway. Key players in these pathways, such as the Bcl2 family proteins, cytochrome c, Apaf-1, caspases, death receptor adapter proteins, and inhibitor of apoptosis proteins, are presented from both functional and structural angles. Until only a few years ago, programmed cell death has been considered essentially synonymous with apoptosis. However, we now know that programmed cell death can also take other forms such as necrosis or necroptosis, and to this end, the mechanisms that underlie programmed necrosis in development and host defense are illustrated. The past twenty plus years have seen an incredible growth of research in cell death, with one breakthrough after another, and the legacy still goes on with constant new surprises and findings. Long live cell death!
  • v. 1-, 1999- Springer
    Al-Rubeai, Mohamed.
  • 2012 ScienceDirect
    [edited by] Nicholas Sperelakis.
    Sect. I. Biophysical chemistry, metabolism, second messengers, and ultrastructure. Biophysical chemistry of physiological solutions / Jeffrey c. Freedman -- Physiological structure and function of proteins / Matthew R. Pincus -- Cell membranes / Jeffrey C. Freedman -- Ionophores in planar lipid bilayers / Jeffrey C. Freedman -- Cell structure / Michael S. Forbes -- Signal transduction and second messengers / Aldebaran M. Hofer -- Calcium as an intracellular second messenger: mediation by calcium-binding proteins / John R. Dedman and Marcia A. Kaetzel -- Sect. II. Membrane potential, transport physiology, pumps, and exchangers. Diffusion and permeability / Nicholas Sperelakis and Jeffrey C. Freedman -- Origin of resting membrane potentials / Nicholas Sperelakis -- Gibbs-Donnan equilibrium potentials / Nicholas Sperelakis -- Mechanisms of carrier-mediated transport: facilitated diffusion, cotransport and countertransport / Steven M. Grassl -- Active ion transport by ATP-driven ion pumps / Robert A. Farley -- Ca2+ -ATPases / Tracy J. Pritchard, Istvan Edes and Evangelia G. Kranias -- Na+ -Ca2+ exchange currents / John H.B. Bridge, Natalia S. Torres and Michela Ottolia -- Intracellular chloride regulation / Francisco J. Alvarez-Leefmans -- Osmosis and regulation of cell volume / Clive M. Baumgarten and Joseph J. Feher -- Intracellular pH regulation / Robert W. Putnam -- Sect. III. Membrane excitability and ion channels. Cable properties and propagation of action potentials / Nicholas sperelakis -- Electrogenesis of membrane excitability / Nicholas Sperelakis -- Patch-clamp techniques / Laura Conforti -- Structure and mechanism of voltage-gated ion channels / Simon Rock Levinson and William A. Sather -- Biology of gap junctions / Richard D. Veenstra -- Regulation of cardiac ion channels by cyclic nucleotide-dependent phosphorylation / Gordon M. Wahler and Nicholas Sperelakis -- Direct regulation of ion channels by GTP-binding proteins / Atsushi Inanobe and Yoshihisa Kurachi -- Developmental changes in ion channels / Takeshi Kobayashi ... [et al.] -- Regulation of ion channel localization and activity through interactions with the cytoskeleton / Stephen Lambert -- Why are so many ion channels mechanosensitives? / Catherine E. Morris -- Sect. IV. Ion channels as targets for toxins, drugs, and genetic diseases. Ion channels as targets for toxins / Kenneth M. Blumenthal -- Ion channels as targets for drugs / Seth Robey, Kevin J. Sampson and Robert S. Kass -- Inherited diseases of ion transport / Robert A. Farley -- Sect. V. Synaptic transmission and sensory transduction. Ligand-gated ion channels / Kenneth R. Tovar and Gary L. Westbrook -- Synaptic transmission / Janusz B. Suszkiw -- Excitation-secretion coupling / Nicole Gallo-Payet and Marcel Daniel Payet -- Stimulus-response coupling in metabolic sensor cells / Stan Misler -- Cyclic nucleotide-gated ion channels / Anita L. Zimmerman -- Sensory receptors and mechanotransduction / Andrew S. French and Päivi H. Torkkeli -- Acoustic transduction / Daniel C. Marcus -- Visual transduction / Anita L. Zimmerman -- Gustatory and olfactory sensory transduction / Stephen D. Roper -- Infrared sensory organs / Stephen D. Roper and Michael S. Grace -- Electroreceptors and magnetoreceptors / Timothy C. Tricas and Bruce A. Carlson -- Sect. VI. Muscle and other contractile systems. Skeletal muscle excitability / Nicholas Sperelakis, Judith Heiny and Hugo gonzalez-Serratos -- Cardiac action potentials / Gordon M. Wahler -- Smooth muscle excitability / Neil D. Detweiler ... [et al.] -- Excitation-contraction coupling in skeletal muscle / Judith A. Heiny and Gerhard Meissner -- Contraction of muscles: mechanochemistry / Richard J. Paul -- Flagella, cilia, actin- and centrin-based movement / Kenneth W. Foster -- Electrocytes of electric fish / Anthony L. Gotter, Marcia A. Kaetzel and John R. Dedman -- Sect. VII. Protozoa and bacteria. Physiological adaptations of protists / Michael Levandowsky -- Physiology of prokaryotic cells / Dennis W. Grogan -- Sect. VIII. Specialized processes: photosynthesis and bioluminescence. Photosynthesis / Darrell Fleischman -- bioluminescence / J. Woodland Hastings.
  • 2011 Springer
    Yasushi Sako, Masahiro Ueda, editors.
  • 2011 Springer Protocols
    edited by Ella Palmer.
    Cell-Based Microarrays: Overview / Ella Palmer -- Cell-Based Co-transfection Microarrays for Use with HEK293T Cells on a Poly d-Lysine-Coated Polystyrene Microplate / Meenal Soni, Fang Lai -- Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays / Ella Palmer, Tom C. Freeman -- A Novel Fluorescent Transcriptional Reporter for Cell-Based Microarray Assays / Tanya M. Redmond, Michael D. Uhler -- High-Throughput Subcellular Protein Localization Using Transfected-Cell Arrays: Subcellular Protein Localization Using Cell Arrays / Yuhui Hu, Michal Janitz -- Cell Arrays for the Measurement of Organelle Dynamics in Living Cells / Holger Erfle, Tautvydas Lisauskas, Christoph Claas, Jürgen Reymann, Vytaute Starkuviene -- High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays / Wei Niu, G. Traver Hart, Edward M. Marcotte -- Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA) / Hansjürgen Volkmer, Frank Weise -- Reverse Transfected Cell Microarrays in Infectious Disease Research / Andreas Konrad, Ramona Jochmann, Elisabeth Kuhn, Elisabeth Naschberger, Priya Chudasama, Michael Stürzl -- Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies / Baochuan Lin, James B. Delehanty -- Blood Cell Capture on Antibody Microarrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance Imaging / Yoann Roupioz, Sarah Milgram, André Roget, Thierry Livache -- Immobilized Culture and Transfection Microarray of Non-adherent Cells / Satoshi Yamaguchi, Erika Matsunuma, Teruyuki Nagamune -- Plasma Polymer and PEG-Based Coatings for DNA, Protein and Cell Microarrays / Andrew L. Hook, Nicolas H. Voelcker, Helmut Thissen -- Polymer Microarrays for Cellular High-Content Screening / Salvatore Pernagallo, Juan J. Diaz-Mochon -- High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation / Koichi Kato, Hiroo Iwata -- Microfluidic Image Cytometry / Ken-ichiro Kamei, Jing Sun, Hsian-Rong Tseng, Robert Damoiseaux.
  • 2006. Springer
    [edited by] Frantisek Baluska, Dieter Volkmann, Peter W. Barlow.
    Cell-cell channels and their implications for cell theory / Frantisek Baluska, Dieter Volkmann, and Peter W. Barlow -- Mating cell-cell channels in conjugating bacteria / Elisabeth Grohmann -- The tetrahymena conjugation junction / Eric S. Cole -- Cytoplasmic bridges in volvox and its relatives / Harold J. Hoops, Ichiro Nishii, and David L. Kirk -- Vegetative hyphal fusion in filamentous fungi / Nick D. Read and M. Gabriela Roca -- Plasmodesmata: cell-cell channels in plants / Yoselin Benitez Alfonso, Laurence Cantrill, and David Jackson -- Sieve-pore plugging mechanisms / Aart J.E. van Bel -- Actin and myosin VIII in plant cell-cell channels / Jozef Samaj ... [et al.] -- Cell-cell communication in wood / John R. Barnett -- TMV movement protein targets cell-cell channels in plants and prokaryotes: possible roles of tubulin- and FtsZ-based cytoskeletons / Manfred Heinlein -- Viral movement proteins induce tubule formation in plant and insect cells / Jan W.M. van Lent and Corinne Schmitt-Keichinger -- Cell-cell movements of transcription factors in plants / Alexis Maizel -- Gap junctions: cell-cell channels in animals / Fabio Mammano -- Tunneling nanotubes: membranous channels between animal cells / Hans-Hermann Gerdes and Amin Rustom -- Cytoplasmic bridges as cell-cell channels of germ cells / Sami Ventelä -- Fusome as a cell-cell communication channel of drosophila ovarian cyst / Jean-René Huynh -- Cytonemes as cell-cell channels in human blood cells / Svetlana Ivanovna Galkina .. [et al.] -- Paracellular pores in endothelial barriers / Luca Manzi and Gianfranco Bazzoni -- Channels across endothelial cells / Radu V. Stan -- Molecular transfers through transient lymphoid cell-cell channels / Mary Poupot, Julie Gertner, and Jean-Jacques Fournié -- Cell-cell transport of homeoproteins: with or without channels / Alain Joliot and Alain Prochiantz -- Virological synapse for cell-cell spread of viruses / Eduardo Garcia and Vincent Piguet -- Cell-cell fusion: transient channels leading to plasma membrane merger / William A. Mohler.
  • 2013 Springer Protocols
    edited by Troy A. Baudino.
    Proteomics analysis of contact-initiated eph receptor-ephrin signaling / Claus Jorgensen and Alexei Poliakov -- Control of vascular tube morphogenesis and maturation in 3D extracellular matrices by endothelial cells and pericytes / George E. Davis ... [and 8 others] -- Analyzing cell-cell interactions in 3-dimensional adhesion assays / Stephanie L.K. Bowers and Troy A. Baudino -- Production of spontaneously beating neonatal rat heart tissue for calcium and contractile studies / Fnu Gerilechaogetu ... [and 6 others] -- Paracrine communication between mechanically stretched myocytes and fibroblasts / Hao Feng ... [and 6 others] -- Assessing blood-brain barrier function using in vitro assays / Joseph Bressler, Katherine Clark, and Cliona O'Driscoll -- Methods to assess tissue permeability / Juan C. Ibla and Joseph Khoury -- In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature / F. Bartsch ... [and 4 others] -- Cell membrane vesicles as a tool for the study of direct epithelial-stromal interaction: lessons from CD147 / Eric Gabison ... [and 3 others] -- Microencapsulation of stem cells to study cellular interactions / Keith Moore, Adam Vandergriff, and Jay D. Potts -- Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies / Timothy N. Feinstein -- Single cell analysis of lipid rafts / William T. Lee -- Micropatterning cell adhesion on polyacrylamide hydrogels / Jian Zhang ... [and 3 others] -- Measuring cell-cell tugging forces using bowtie-patterned mPADs (microarray post detectors) / Daniel M. Cohen, Mike T. Yang, and Christopher S. Chen -- Generation and analysis of biosensors to measure mechanical forces within cells / Katharina Austen ... [and 4 others] -- Proteomic analysis of the left ventricle post-myocardial infarction to identify in vivo candidate matrix metalloproteinase substrates / Andriy Yabluchanskiy ... [and 5 others].
  • 2006 Springer Protocols
    edited by Sean P. Colgan.
    Analysis of mammallian sperm-egg membrane interactions during in vitro fertilization / Genevieve B. Wortzman, Allison J. Gardner, and Janice P. Evans -- Collagen gel contraction assay / Peter Ngo ... [et al.] -- Methods to assess tissue permeability / Juan C. Ibla and Joseph Khoury -- Bacterial-bacterial cell interactions in biofilms: detection of polysaccharide intercellular adhesins by blotting and confocal microscopy / Kimberly K. Jefferson and Nuno Cerca -- A biochemical method for tracking cholera toxin transport from plasma membrane to golgi and endoplasmic reticulum / Heidi E. DeLuca and Wayne I. Lencer -- Isolation and culture of murine heart and lung endothelial cells for in vitro model systems / Yaw-Chyn Lim and Frances W. Luscinskas -- Blood-brain barrier and cell-cell interactions: methods for establishing in vitro models of the blood/brain barrier and transport measurements / Michael Aschner ... [et al.] -- Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences / Geraldine Canny, Alexander Swidsinski, and Beth A. McCormick -- Expression cloning of signaling proteins regulated by cell adhesion / Michelle L. Matter and Joe W. Ramos -- Assays for the calcium sensitivity of desmosomes / Anita J. Merritt, Anthea Scothern, and Tanusree Bhattacharyya -- Tight junctions and cell-cell interactions / Markus Utech, Matthias Brüwer, and Asma Nusrat -- Simultaneous immunofluorescence detection of cadherin and [beta]-catenin in mouse tissue sections / Nobuko Obara -- In vitro neutrophil transepithelial migration / Winston Y. Lee ... [et al.] -- The blot running assay: a method for identifying adhesion molecules mediating binding under shear conditions / Robert Sackstein and Robert Fuhlbrigge -- Cell-cell interaction in the transcellular biosynthesis of novel [omega]-3-derived lipid mediators / Nan Chiang and Charles N. Serhan -- Isolation and analysis of lipid rafts in cell-cell interactions / Aimee Landry and Ramnik Xavier. The role of junctional adhesion molecules in interactions between vascular cells / Triantafyllos Chavakis and Valeria Orlova -- Cell-cell interactions on solid matrices / Nancy A. Louis, Dionne Daniels, and Sean P. Colgan -- Cell-cell interactions in the kidney: inducible expression of mutant G protein [alpha]-subunits in madin-darby cannine kidney cells for studies of epithelial cell junction structure and function / Ernesto Sabath and Bradley M. Denker -- Nucleotide metabolism and cell-cell interactions / Holger K. Eltzschig ... [et al.] --
  • 2015 Springer Protocols
    edited by Ülo Langel.
    Classes of cell-penetrating peptides / Margus Pooga and Ülo Langel -- Penetratin story : an overview / Edmond Dupont, Alain Prochiantz, and Alain Joliot -- Prediction of cell-penetrating peptides / Mattias Hällbrink and Mati Karelson -- Computer-aided virtual screening and designing of cell- penetrating peptides / Ankur Gautam, Kumardeep Chaudhary, Rahul Kumar, and Gajendra Pal Singh Raghava -- Investigating membrane interactions and structures of CPPs / Fatemeh Madani and Astrid Gräslund -- Determining the effects of membrane-interacting peptides on membrane integrity / William C. Wimley -- Study of CPP mechanisms by mass spectrometry / Sandrine Sagan, Chérine Bechara, and Fabienne Burlina -- Methods to study the role of the glycocalyx in the uptake of cell-penetrating peptides / Samuel Schmidt, Rike Wallbrecher, Toin H. van Kuppevelt, and Roland Brock -- Toxicity, immunogenicity, uptake, and kinetics methods for CPPs / Julia Uusna, Kent Langel, and Ülo Langel -- Unraveling the mechanisms of peptide-mediated delivery of nucleic acids using electron microscopy / Helerin Margus, Carmen Juks, and Margus Pooga -- SCARA involvement in the uptake of nanoparticles formed by cell-penetrating peptides / Henrik Helmfors, Staffan Lindberg, and Ülo Langel -- Protein mimicry and the design of bioactive Cell- penetrating peptides / John Howl and Sarah Jones -- Pepducins and other lipidated peptides as mechanistic probes and therapeutics / Ping Zhang, Lidija Covic, and Athan Kuliopulos -- Identification and characterization of homing peptides using in vivo peptide phage display / Maija Hyvönen and Pirjo Laakkonen -- Antimicrobial and antiviral applications of cell- penetrating peptides / Kalle Pärn, Elo Eriste, and Ülo Langel -- Visualizing actin architectures in cells incubated with cell-penetrating peptides / Lin He, Peter D. Watson, and Arwyn T. Jones -- Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro / Stine Rønholt, Mie Kristensen, and Hanne Mørck Nielsen -- Pathway toward tumor cell-selective CPPs? / Isabel D. Alves, Manon Carré, and Solange Lavielle -- PepFects and nickFects for the intracellular delivery of nucleic acids / Piret Arukuusk, Ly Pärnaste, Mattias Hällbrink, and Ülo Langel -- In vitro assays to assess exon skipping in duchenne muscular dystrophy / Prisca Boisguerin, Liz O'Donovan, Michael J. Gait, and Bernard Lebleu -- Applications of ApoB LDLR-binding domain approach for the development of CNS-penetrating peptides for alzheimer's disease / Eliezer Masliah and Brian Spencer -- CPP-based delivery system for in vivo gene delivery / Kaido Kurrikoff, Kadi-Liis Veiman, and Ülo Langel -- Application of CPPs for brain delivery / Artita Srimanee, Jakob Regberg, and Ülo Langel -- Intracellular delivery of nanoparticles with cell penetrating peptides / Giuseppina Salzano and Vladimir P. Torchilin -- Multifunctional oligoaminoamides for the receptor-specific delivery of therapeutic RNA / Judith Weber, Ulrich Lächelt, and Ernst Wagner -- Cell penetrating peptides for chemical biological studies / Ikuhiko Nakase, Toshihide Takeuchi, and Shiroh Futaki -- Experiences with CPP-based self assembling peptide systems for topical delivery of botulinum toxin / Jane Lee, Phil Kennedy, and Jacob M. Waugh -- Applications of CPPs in genome modulation of plants / Alicja Ziemienowicz, Jordan Pepper, and François Eudes -- DNA transfer into animal cells using stearylated CPP based Transfection Reagent / Kristiina Karro, Tiiu Männik, Andres Männik, and Mart Ustav -- Live cell genomics : cell-specific transcriptome capture in live tissues and cells / Thomas J. Bell and James Eberwine -- Live cell genomics : RNA exon-specific RNA-binding protein isolation / Thomas J. Bell and James Eberwine.
  • 2011 Springer Protocols
    edited by Ülo Langel.
    Classes and prediction of cell-penetrating peptides -- Penetratin story: An overview -- Testing membrane interactions of CPPs -- Interactions of amphipathic CPPs with model membranes -- NMR studies of three-dimensional structure and positioning of CPPs in membrane model systems -- Measurements of the intracellular stability of CPPs -- Tools for predicting binding and insertion of CPPs into lipid bilayers -- Studies of proteoglycan involvement in CPP-mediated delivery -- Uptake kinetics of cell-penetrating peptides -- Thermodynamics of lipid interactions with cell-penetrating peptides -- Calcium and membrane repair -- Mapping of protein transduction pathways with fluorescent microscopy -- Insight into cell-entry mechanisms of CPPs by electron microscopy -- Toxicity methods for CPPs -- Comparison of CPP uptake methods -- Characterization of cellular internalization pathways for CPP-mediated oligonucleotide delivery -- Mimicry of protein function with cell-penetrating peptides -- Homeoprotein intercellular transfer, the hidden face of cell-penetrating peptides -- Pharmacology, biodistribution, and efficacy of GPCR-based pepducins in disease models -- Identification and characterization of tissue-specific protein transduction domains using peptide phage display -- Applications of cell-penetrating peptides as signal transduction modulators for the selective induction of apoptosis -- Splice redirection as a convenient assay to monitor CPP-on efficiency and mechanism -- CPP-directed oligonucleotide exon skipping in animal models of Duchenne muscular dystrophy -- PTD-DRBD siRNA delivery -- A non-covalent peptide-based strategy for siRNA delivery -- Application of pepfect peptides for the delivery of splice-correcting oligonucleotides -- Internalization of nucleoside phosphates into live cells by complex formation with different CPPs and JBS-nucleoducin -- Enhanced cellular delivery of cell-penetrating peptide-peptide nucleic acid conjugates by photochemical internalization -- Identification of homing peptides using the in vivo phage display technology -- Measuring the action of CPP-siRNA conjugates in the lung -- Intracellular delivery of nanoparticles with CPPs -- Multifunctional CPP polymer system for tumor-targeted pDNA and siRNA delivery -- Cell-penetrating penta-peptides and bax-inhibiting peptides: Protocol for their application -- Pair technology: Exon-specific rna-binding protein isolation in live cells -- Quantitation of cellular and topical uptake of luciferin-oligoarginine conjugates -- Industrial-scale manufacturing of a possible oligonucleotide cargo CPP-based drug -- Application of a fusiogenic peptide GALA for intracellular delivery -- Therapeutic applications of cell-penetrating peptides -- Nonclinical and clinical experiences with CPP-based self-assembling peptide systems in topical drug development.
  • 2013 Springer Protocols
    edited by Race L. Kao.
    Cellular cardiomyoplasty : its past, present, and future / Elizabeth K. Lamb, Grace W. Kao, and Race L. Kao -- Skeletal muscle stem cells / Grace W. Kao, Elizabeth K. Lamb, and Race L. Kao -- Bone marrow stem cells / Minh Ngoc Duong, Yu-Ting Ma, and Ray C.J. Chiu -- Adipose tissue-derived mesenchymal stem cells : isolation, expansion and characterization / Miriam Araña [and 4 others] -- Cardiac side population cells and Sca-1-positive cells / Toshio Nagai, Katsuhisa Matsuura, and Issei Komuro -- Two-step protocol for isolation and culture of cardiospheres / Lijuan Chen [and 5 others] -- Generation of human iPSCs from human peripheral blood mononuclear cells using non-integrative sendai virus in chemically defined conditions / Jared M. Churko, Paul W. Burridge, and Joseph C. Wu -- Identification of stem cells after transplantation / Yingjie Wang [and 5 others] -- Methods to study the proliferation and differentiation of cardiac side population (CSP) cells / Konstantina-Ioanna Sereti [and 3 others] -- Immune responses after mesenchymal stem cell implantation / Rony Atoui and Ray C.J. Chiu -- Route of delivery, cell retention, and efficiency of polymeric microcapsules in cellular cardiomyoplasty / Alice Le Huu [and 3 others] -- Angiogenic nanodelivery systems for myocardial therapy / Arghya Paul, Dominique Shum-Tim, and Satya Prakash -- Bio-hybrid tissue engineering for cellular cardiomyoplasty : future directions / Juan Carlos Chachques -- Decellularized whole heart for bioartificial heart / Hug Aubin [and 4 others] -- Clinical trials of cardiac repair with adult bone marrow-derived cells / Vinodh Jeevanantham [and 3 others] -- Clinical study using adipose-derived mesenchymal-like stem cells in acute myocardial infarction and heart failure / Ilia Alexander Panfilov [and 3 others].
  • 2012 Springer
    Brian Henderson, A. Graham Pockley, editors.
    Section 1 Historical Introduction to Secreted Cell Stress Proteins as Signalling Proteins -- Discovery of the Cellular Secretion of Cell Stress Proteins -- Discovery of the Agonist Activities of Molecular Chaperones and Protein-Folding Catalysts -- Identification of Cell Stress Proteins in Biological Fluids -- Section 2 Intracellular Trafficking of Molecular Chaperones and its Consequences -- Hsp27 Phosphorylation Patterns and Cellular Consequences -- Evidence on Cholesterol-Controlled Lipid Raft Interaction of the Small Heat Shock Protein HSPB11 -- Hsp70 Chaperone Systems in Vesicular Trafficking -- Pathways of Hsp70 Release: Lessons from Cytokine Secretion -- Nucleolin: A Novel Intracellular Transporter of HSPA1A -- The Hsp90-Based Protein Trafficking System and Linkage to Protein Quality Control -- Section 3 Molecular Chaperones as Cell Surface Receptors and Receptor Ligands -- Cell Surface Molecular Chaperones and the LPS Receptor -- Hsp60: An Unexpected Cell Surface Receptor in Prokaryotes and Eukaryotes -- Pathophysiological Barriers Impeding the Delivery of Heat Shock Protein (HSP)-based Macromolecules and Nanotherapeutics to Solid Tumors -- The Chaperokine Activity of HSPA1A -- Molecular Chaperones and Scavenger Receptors: Binding and Trafficking of Molecular Chaperones by Class F and Class H Scavenger Receptors -- Grp78 (BiP): A Multifunctional Cell Surface Receptor -- Section 4 Extracellular Secretion of Molecular Chaperones in Prokaryotes and Eukaryotes -- Mycobacterium Tuberculosis Hsp60 as a Key Virulence Factor in Tuberculosis -- Hsp90 vs Conventional Growth Factors in Acute and Diabetic Wound Healing -- Circulating Molecular Chaperones in Health and Disease -- Index.
  • 2013 Springer
    Alberto J. L. Macario, Everly Conway de Macario, Francesco Cappello.
    This Brief provides a concise review of chaperonopathies, i.e., diseases in which molecular chaperones play an etiologic-pathogenic role. Introductory chapters deal with the chaperoning system and chaperoning teams and networks, HSP-chaperone subpopulations, the locations and functions of chaperones, and chaperone genes in humans. Other chapters present the chaperonopathies in general, including their molecular features and mechanistic classification into by defect, excess, or mistake. Subsequent chapters discuss the chaperonopathies in more detail, focusing on their distinctive characteristic.
  • 2005 Springer Protocols
    edited by Edward D. Zanders.
    Also available: Print – 2005
  • 2006 Springer
    S. Jaroch, H. Weinmann, editors.
  • 2013 CRCnetBASE
    edited by Ferenc Darvas, Andras Guttman, Gyorgy Dorman.
    Ch. 1. Utilizing small molecules in chemical genomics : toward high-throughput (HT) approaches / György Dormán and Ferenc Darvas -- ch. 2. Development and application of novel analytical methods in lipidomics / Yuqin Wang ... [et al.] -- ch. 3. From chemical genomics to chemical proteomics : the power of microarray technology / Sándor Spisák and András Guttman -- ch. 4. Genomic and proteomic biomarkers in the drug R&D process / László Takács, Anna Debreceni, and István Kurucz -- ch. 5. Quo vadis biomedical sciences in the omics era : toward computational biology and medicine / András Falus ... [et al.] -- ch. 6. Novel chemogenomic approaches to drug design / Didier Rognan.
  • 2011 Springer Protocols
    edited by Joe Zhongxiang Zhou.
    Historical overview of chemical library design / Roland E. Dolle -- Chemoinformatics and library design / Joe Zongxiang Zhou -- Molecular library design using multi-objective optimization methods / Christos A. Nicolaou and Christos C. Kannas -- A scalable approach to combinatorial library design / Puneet Sharma, Srinivasa Salapaka, and Carolyn Beck -- Application of Free-Wilson selectivity analysis for combinatorial library design / Simone Sciabola ... [et al.] -- Application of QSAR and shape pharmacophore modeling approaches for targeted chemical library design / Jerry O. Ebalunode, Weifan Zheng, and Alexander Tropsha -- Combinatorial library design from reagent pharmacophore fingerprints / Hongming Chen, Ola Engkvist, and Niklas Blomberg -- Docking methods for structure-based library design / Claudio N. Cavasotto and Sharangdhar S. Phatak -- Structure-based library design in efficient discovery of novel inhibitors / Shunqi Yan and Robert Selliah -- Structure-based and property-compliant library design of 11[beta]-HSD1 adamantyl amide inhibitors / Genevieve D. Paderes ... [et al.] -- Design of screening collections for successful fragment-based lead discovery / James Na and Qiyue Hu -- Fragment-based drug design / Eric Feyfant ... [et al.] -- LEAP into the Pfizer Global Virtual Library (PGVL) space : creation of readily synthesizable design ideas automatically / Qiyue Hu ... [et al.] -- The design, annotation, and application of a kinase-targeted library / Hualin Xi and Elizabeth A. Lunney -- PGVL hub : an integrated desktop tool for medicinal chemists to streamline design and synthesis of chemical libraries and singleton compounds / Zhengwei Peng ... [et al.] -- Design of targeted libraries against the human Chk1 kinase using PGVL hub / Xengwei Peng and Qiyue Hu -- GLARE : a tool for product-oriented design of combinatorial libraries / Jean-François Truchon -- CLEVER : a general design tool for combinatorial libraries / Tze Hau Lam ... [et al.].
  • 2013 Springer Protocols
    edited by Matthew R. Banghart.
    Engineering k(+) channels using semisynthesis -- Chemical derivatization and purification of peptide-toxins for probing ion channel complexes -- Using yeast to study potassium channel function and interactions with small molecules -- A FLIPR assay for evaluating agonists and antagonists of GPCR heterodimers -- Characterizing caged molecules through flash photolysis and transient absorption spectroscopy -- Characterization of one- and two-photon photochemical uncaging efficiency -- Photochromic potassium channel blockers: Design and electrophysiological characterization -- A (1)h NMR assay for measuring the photostationary states of photoswitchable ligands -- Developing a photoreactive antagonist -- Development and in vitro characterization of ratiometric and intensity-based fluorescent ion sensors -- Characterization of voltage-sensitive dyes in living cells using two-photon excitation -- Characterization and validation of fluorescent receptor ligands: A case study of the ionotropic serotonin receptor -- Imaging single synaptic vesicles in mammalian central synapses with quantum dots -- Directed evolution of protein-based neurotransmitter sensors for MRI.
  • v. 1-3, 1970.
    edited by Endre A. Balazs.
    Status: Not Checked OutLane Catalog Record
    v. 1. Collagen, basal laminae elastin --v. 2. Glycosaminoglycans and proteoglycans --v. 3. Structural organization and function of the matrix.
  • 2016 ScienceDirect
    edited by Tracy M. Handel.
  • 2013 Springer Protocols
    edited by Astrid E. Cardona, Eroboghene E. Ubogu.
    Chemokines constitute a large family of structurally similar cytokines that contain a signature of conserved cysteine residues joined by disulfide bridges. Binding of chemokines to specific G protein-coupled receptors followed by downstream signaling defines their biological function. Initially, chemoattraction was the key function linked to chemokines/chemokine receptors; however, in recent years, it has become clear that chemokine ligand-receptor interactions can also modulate cellular activation, survival, and proliferation, among other functions in homeostatic and diseased states. Importantly, major advances in our understanding of chemokine biology have led to chemokine receptors becoming specific therapeutic targets with great potential. In Chemokines: Methods and Protocols, expert researchers provide practical information regarding experimental models and state of the art protocols used to delineate chemokine/chemokine receptor function and their applications in health and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Chemokines: Methods and Protocols aims to reveal key protocols of functional and descriptive chemokine ligand/receptor assays that will be of practical significance to graduate students, post-doctoral fellows, trainees, and researchers in academia and industry.
  • 2012 Springer Protocols
    edited by Randall H. Morse.
    Strain construction and screening methods for a yeast histone H3/H4 mutant library / Junbiao Dai and Jef D. Boeke -- Measuring dynamic changes in histone modifications and nucleosome density during activated transcription in budding yeast / Chhabi K. Govind, Daniel Ginsburg, and Alan G. Hinnebusch -- Monitoring the effects of chromatin remodelers on long-range interactions in vivo / Christine M. Kiefer and Ann Dean -- Measuring nucleosome occupancy in vivo by micrococcal nuclease / Gene O. Bryant -- Analysis of nucleosome positioning using a nucleosome-scanning assay / Juan Jose Infante, G. Lynn Law, and Elton T. Young -- Assaying chromatin structure and remodeling by restriction enzyme accessibility / Kevin W. Trotter and Trevor K. Archer -- Generation of DNA circles in yeast by inducible site-specific recombination / Marc R. Gartenberg -- An efficient purification system for native minichromosome from Saccharomyces cerevisiae / Ashwin Unnikrishnan [and others] -- Simultaneous single-molecule detection of endogenous C-5 DNA methylation and chromatin accessibility using MAPit / Russell P. Darst [and others] -- Analysis of stable and transient protein-protein interactions / Stephanie Byrum [and others] -- Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching / Florian Mueller [and others] -- Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy / Davide Mazza [and others] -- Analysis of chromatin structure in plant cells / Mala Singh [and others] -- Analysis of histones and histone variants in plants / Ila Trivedi [and others] -- Reconstitution of modified chromatin templates for in vitro functional assays / Miyong Yun [and others] -- A defined in vitro system to study ATP-dependent remodeling of short chromatin fibers / Verena K. Maier and Peter B. Becker -- In vitro reconstitution of in vivo-like nucleosome positioning on yeast DNA / Christian J. Wippo and Philipp Korber -- Activator-dependent acetylation of chromatin model systems / Heather J. Szerlong and Jeffrey C. Hansen -- Mapping assembly favored and remodeled nucleosome positions on polynucleosomal templates / Hillel I. Sims, Chuong D. Pham, and Gavin R. Schnitzler -- Analysis of changes in nucleosome conformation using fluorescence resonance energy transfer / Tina Shahian and Geeta J. Narlikar -- Preparation of nucleosomes containing a specific H2A-H2A cross-link forming a DNA-constraining loop structure / Ning Liu and Jeffrey J. Hayes -- Sulfyhydryl-reactive site-directed cross-linking as a method for probing the tetrameric structure of histones H3 and H4 -/ Andrew Bowman and Tom Owen-Hughes -- Genomic approaches for determining nucleosome occupancy in yeast Kyle Tsui [and others] -- Genome-wide approaches to determining nucleosome occupancy in metazoans using MNase-Seq / Kairong Cui and Keji Zhao -- Salt fractionation of nucleosomes for genome-wide profiling / Sheila S. Teves and Steven Henikoff -- Quantitative analysis of genome-wide chromatin remodeling / Songjoon Baek, Myong-Hee Sung, and Gordon L. Hager -- Computational analysis of nucleosome positioning / Itay Tirosh.
  • 2015 Springer Protocols
    edited by Shondra M. Pruett-Miller.
    Genome editing by targeted chromosomal mutagenesis -- Piggybac transposon-based insertional mutagenesis in mouse haploid embryonic stem cells -- Using phage integrases in a site-specific dual integrase cassette exchange strategy -- Therapeutic genome mutagenesis using synthetic donor DNA and triplex-forming molecules -- Genome engineering using adeno-associated virus (AAV) -- Engineering of customized meganucleases via in vitro compartmentalization and in cellulo optimization -- Efficient design and assembly of custom TALENs using the golden gate platform -- Ligation-independent cloning (LIC) assembly of TALEN genes -- Assembly and characterization of megatals for hyperspecific genome engineering applications -- Genome engineering using CRISPR-Cas9 system -- Donor plasmid design for codon and single base genome editing using zinc finger nucleases -- Endogenous gene tagging with fluorescent proteins -- Silencing long noncoding RNAs with genome-editing tools -- Gene editing using ssODNs with engineered endonucleases -- Genome editing in human pluripotent stem cells using site-specific nucleases -- Strategies to increase genome editing frequencies and to facilitate the identification of edited cells -- Using engineered endonucleases to create knockout and knockin zebrafish models -- Creating knockout and knockin rodents using engineered endonucleases via direct embryo injection -- Simple sperm preservation by freeze-drying for conserving animal strains.
  • 2008 Springer Protocols
    edited by Gregory D. Davis, Kevin J. Kayser.
    Biallelic gene knockouts in Chinese hamster ovary cells / Naoko Yamane-Ohnuki, Kazuya Yamano, and Mitsuo Satoh -- Highly proficient gene targeting by homologous recombination in the human pre-B cell line Nalm-6 / Noritaka Adachi, Aya Kurosawa, and Hideki Koyama -- Robust cell line development using meganucleases / Jean-Pierre Cabaniols and Frédéric Pâques -- Design and testing of zinc finger nucleases for use in mammalian cells / Matthew Porteus -- Gene targeting in Drosophila and Caenorhabditis elegans with zinc-finger nucleases / Dana Carroll ... [et al.] -- Orpheus recombination : a comprehensive bacteriophage system for murine targeting vector construction by transplacement / Knut Woltjen ... [et al.] -- Transposition -mediated mutagenesis in somatic cells : identification of transposon-genomic DNA junctions / David A. Largaespada and Lara S. Collier -- Insertional mutagenesis of the mouse germline with sleeping beauty transposition / Junji Takeda, Zsuzsanna Izsvák, and Zoltán Ivics -- Conditional gene trapping using the FLEx system / Thomas Floss and Frank Schnütgen -- Steps toward targeted insertional mutagenesis with class II transposable elements / Sareina Chiung-Yuan wu ... [et al.] -- Targeting integration of the Saccharomyces Ty5 retrotransposon / Troy L. Brady, Clarice L. Schmidt, and Daniel F. Voytas -- Site-specific chromosomal integration mediated by [phi]C31 integrase / Annahita Keravala and Michele P. Calos-- Triplex-mediated gene modification / Erica B. Schleifman, Joanna Y.Chin, and Peter M. Glazer -- Allelic exchange of unmarked mutations in Mycobacterium tuberculosis / Martin S. Pavelka, Jr. -- Mycobacterial recombineering Julia C. van Kessel and Graham F. Hatfull -- Chromosomal engineering of Clostridium perfringens using group II introns / Phalguni Gupta and Yue Chen.
  • 2007 Springer Protocols
    edited by Ezio Rosato.
    Basic protocols for drosophila S2 cell line: maintenance and transfection / M. Fernanda Ceriani -- Co-immunoprecipitation on drosophila cells in culture / M. Fernanda Ceriani -- Basic protocols for zebrafish cell lines: maintenance and transfection / Daniela Vallone ... [et al.] -- Manipulation of mammalian cell lines for circadian studies / Filippo Tamanini -- Reporter assays / M. Fernanda Ceriani -- Use of firefly luciferase activity assays to monitor circadian molecular rhythms in vivo and in vitro / Wangjie Yu and Paul E. Hardin -- Suprachiasmatic nucleus cultures that maintain rhythmic properties in vitro / K. Tominaga-Yoshino, T. Ueyama, and H. Okamura -- RNA in situ hybridizations on drosophila whole mounts / Corinna Wulbeck and Charlotte Helfrich-Forster -- In situ hybridization of suprachiasmatic nucleus slices / Horacio O. de la Iglesia -- Immunohistochemistry in drosophila: sections and whole mounts / Charlotte Helfrich-Forster -- Immunocytochemistry on suprachiasmatic nucleus slices / Marta Muñoz Llamosas -- Immunofluorescence analysis of circadian protein dynamics in cultured mammalian cells / Filippo Tamanini. Light, photoreceptors, and circadian clocks / Russell G. Foster, Mark W. Hankins, and Stuart N. Peirson -- Statistical analysis of biological rhythm data / Harold B. Dowse -- Rhythmic conidiation in Neurospora crassa / Cas Kramer -- Monitoring and analyzing drosophila circadian locomotor activity / Mauro A. Zordan, Clara Benna, and Gabriella Mazzotta -- Automated video image analysis of larval zebrafish locomotor rhythms / Gregory M. Cahill -- Locomotor activity in rodents / Gianluca Tosini -- Analysis of circadian leaf movement rhythms in Arabidopsis thaliana / Kieron D. Edwards and Andrew J. Millar -- Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria / Shannon R. Canales ... [et al.] -- Analysis of rhythmic gene expression in adult drosophila using the firefly-luciferase reporter gene / Ralf Stanewsky -- Monitoring circadian rhythms in Arabidopsis thaliana using luciferase reporter genes / Anthony Hall and Paul Brown -- Specialized techniques for site-directed mutagenesis in cyanobacteria / Eugenia M. Clerico, Jayna L. Ditty, and Susan S. Golden -- Novel strategies for the identification of clock genes in neurospora with insertional mutagenesis / Kruno Sveric ... [et al.] -- Mutagenesis with drosophila / Patrick Emery -- Mutagenesis in arabidopsis / Jodi Maple and Simon G. Moller -- Yeast two-hybrid screening / Jodi Maple and Simon G. Moller -- Microarrays: quality control and hybridization protocol / Ken-ichiro Uno and Hiroki R. Ueda -- Microarrays: statistical methods for circadian rhythms / Rikuhiro Yamada and Hiroki R. Ueda -- Identification of clock genes using difference gel electrophoresis / Natasha A. Karp and Kathryn S. Lilley -- Isolation of total RNA from Neurospora mycelium / Cas Kramer -- RNA extraction from drosophila heads / Patrick Emery -- Extraction of plant RNA / Michael G. Salter and Helen E. Conlon -- RNA extraction from mammalian tissues / Stuart N. Peirson and Jason N. Butler -- Northern analysis of sense and antisense fequency RNA in Neurospora crassa / Cas Kramer -- RNase protection assay / Patrick Emery -- Quantitative polymerase chain reaction / Stuart N. Peirson and Jason N. Butler -- Protein extraction, fractionation, and purification from cyanobacteria / Natalia B. Ivleva and Susan S. Golden -- Protein extraction from drosophila heads / Patrick Emery -- Plant protein extraction / Helen E. Conlon and Michael G. Salter -- Protein extraction from mammalian tissues / Choogon Lee -- Western blotting / Choogon Lee -- Co-immunoprecipitation assay / Choogon Lee -- In vitro phosphorylation and kinase assays in Neurospora crassa / Lisa Franchi and Giuseppe Macino --
  • 2013 Springer Protocols
    edited by Nobuyoshi Kosaka.
    Functional analysis of exosomal microRNA in cell-cell communication research / Nobuyoshi Kosaka [and others] -- Isolation of extracellular nanovesicle microRNA from liver cancer cells in culture / Takayuki Kogure and Tushar Patel -- Methods of analysis of dendritic cell-derived exosome-shuttle microRNA and its horizontal propagation between dendritic cells / Angela Montecalvo, Adriana T. Larregina, and Adrian E. Morelli -- Analysis of microRNA and protein transfer by exosomes during an immune synapse / Carolina Villarroya-Beltri -- Analysis of viral microRNA exchange via exosomes in vitro and in vivo / Frederik J. Verweij [and others] -- Measurement of precursor miRNA in exosomes from human ESC-cerived mesenchymal stem cells / Tian Sheng Chen and Sai Kiang Lim -- Analysis of the transfer of circulating microRNA between cells mediated by gap junction / Steven J. Greco and Pranela Rameshwar -- Isolation of circulating microRNA associated with RNA-binding protein / Andrey Turchinovich, Ludmila Weiz, and Barbara Burwinkel -- Identification and analysis of circulating exosomal microRNA in human body fluids / Cecilia Lässer -- Analyzing the circulating microRNAs in exosomes/extracellular vesicles from serum or plasma by qRT-PCR / Leni Moldovan [and others] -- Direct serum assay for microRNA in cancer patients / Sota Asaga and Dave S.B. Hoon -- Analysis of microRNA niches : techniques to measure extracellular microRNA and intracellular microRNA in situ / Victoria N. Parikh and Stephen Y. Chan -- Analysis of circulating microRNA by microarray in liver disease / Yoshiki Murakami and Toshihito Tanahashi -- Isolation of circulating microRNA in saliva / Alessia Gallo and Ilias Alevizos -- Purification of RNA from milk whey / Hirohisa Izumi [and others] -- Circulating microRNAs in the cerebrospinal fluid of patients with brain diseases / Akira Machida, Takuya Ohkubo, and Takanori Yokota -- Methods of microRNA quantification in urinary sediment / Gang Wang and Cheuk Chun Szeto -- Circulating microRNA for the identification of forensically relevant body fluids / Erin K. Hanson and Jack Ballantyne -- Identification of prostate cancer-associated microRNAs in circulation using a mouse model of disease / Luke A. Selth [and others] -- Combination of extraction reagent and DNA microarray that allows for the detection of global miRNA profiles from serum/plasma / Makiko Ichikawa and Hideo Akiyama -- Nanopore single-molecule detection of circulating microRNAs / Li-Qun Gu and Yong Wang.
  • 2006 Springer Protocols
    edited by Y.M. Dennis Lo, Rossa W.K. Chiu, K.C. Allen Chan.
    Introduction to the polymerase chain reaction / Y.M. Dennis Lo and K.C. Allen Chan -- Setting up a polymerase chain reaction laboratory / Y.M. Dennis Lo and K.C. Allen Chan -- Real-time polymerase chain reaction and melting curve analysis / Robert J. Pryor and Carl T. Wittwer -- Qualitative and quantitative polymerase chain reaction-based methods for DNA methylation analyses / Ivy H.N. Wong -- In-cell polymerase chain reaction: strategy and diagnostic applications / T. Vauvert Hviid -- Qualitative and quantitative DNA and RNA analysis by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry / Chunming Ding -- Analysis of polymerase chain reaction products by denaturing high-performance liquid chromatography / Ching-Wan Lam -- Use of real-time polymerase chain reaction for the detection of fetal aneuploidies / Bernhard Zimmermann ... [et al.] -- Noninvasive prenatal diagnosis by analysis of fetal DNA in maternal plasma / Rossa W. K. Chiu and Y. M. Dennis Lo -- Clinical applications of plasma Epstein-Barr virus DNA analysis and protocols for the quantitative analysis of the size of circulating Epstein-Barr virus DNA / K.C. Allen Chan and Y.M. Dennis Lo -- Molecular analysis of circulating RNA in plasma / Nancy B.Y. Tsui, Enders K.O. Ng, and Y.M. Dennis Lo -- Molecular analysis of mitochondrial DNA point mutations by polymerase chain reaction / Lee-Jun C. Wong, Bryan R. Cobb, and Tian-Jian Chen -- Novel applications of polymerase chain reaction to urinary nucleic acid analysis / Anatoly V. Lichtenstein ... [et al.] -- Detection and quantitation of circulating plasmodium falciparum DNA by polymerase chain reaction / Shira Gal and James S. Wainscoat -- Molecular diagnosis of severe acute respiratory syndrome / Enders K.O. Ng and Y.M. Dennis Lo -- Genomic sequencing of the severe acute respiratory syndrome-coronavirus / Stephen S.C. Chim, Rossa W.K. Chiu, and Y.M. Dennis Lo.
  • 2014 Wellcome Trust
    edited by E.M. Jones and E.M. Tansey.
  • 2015 Springer Protocols
    edited by Antonia Vlahou, Manousos Makridakis.
    Biological sample collection for clinical proteomics : existing SOPs / Vasiliki Lygirou, Manousos Makridakis, and Antonia Vlahou -- Targeting the proteome of cellular fractions : focus on secreted proteins / Agnieszka Latosinska [and others] -- Preparation of urinary exosomes : methodological issues for clinical proteomics / Marina Pitto, Samuele Corbetta, and Francesca Raimondo -- Sample treatment methods involving combinatorial peptide ligand libraries for improved proteomes analyses / Pier Giorgio Righetti and Egisto Boschetti -- Glycoprotein enrichment method Using a selective magnetic nano-probe platform (MNP) functionalized with lectins / Marta Cova [and others] -- Latest advancements in proteomic two-dimensional gel electrophoresis analysis applied to biological samples / Laura Santucci [and others] -- 2DE maps in the discovery of human autoimmune kidney diseases : the case of membranous glomerulonephritis / Maurizio Bruschi, Laura Santucci, Gian Marco Ghiggeri, and Giovanni Candiano -- MALDI-imaging mass spectrometry on tissues / Veronica Mainini [and others] -- Laser capture microdissection of fluorescently labeled amyloid plaques from alzheimer's disease brain tissue for mass spectrometric analysis / Diana A.T. Nijholt, Christoph Stingl, and Theo M. Luider -- Urine sample preparation and fractionation for global proteome profiling by LC-MS / Magali Court, Jérôme Garin, and Christophe D. Masselon -- Methods in capillary electrophoresis coupled to mass spectrometry for the identification of clinical proteomic/peptidomic biomarkers in biofluids / Angelique Stalmach [and others] -- Quantification of proteins in urine samples using targeted mass spectrometry methods / Nina Khristenko and Bruno Domon -- Statistical issues in the design and planning of proteomic profiling experiments / David A. Cairns -- Integrating proteomics profiling data sets : a network perspective / Akshay Bhat, Mohammed Dakna, and Harald Mischak -- European medicines agency experience with biomarker qualification / Efthymios Manolis [and others].
  • 2008 Springer Protocols
    edited by Antonia Vlahou.
    Overview and introduction to clinical proteomics / Young-Ki Paik ... [et al.] -- Specimen collection and handling : standardization of blood sample collection / Harald Tammen -- Tissue sample collection for proteomics analysis / Jose I. Diaz, Lisa H. Cazares, and O. John Semmes -- Protein profiling of human plasma samples by two-dimensional electrophesis / Sang Yun Cho ... [et al.] -- Analysis of laser capture microdissected cells by 2-dimensional gel electrophoresis / Daohai Zhang and Evelyn Siew-Chuan Koay -- Optimizing the difference gel electrophesis (DIGE) technology / David B. Friedman and Kathryn S. Lilley -- MALDI/SELDI protein profiling of serum for the identification of cancer biomarkers / Lisa H. Cazares ... [et al.] -- Urine sample preparation and protein profiling by two-dimensional electrophoresis and matrix-assisted laser desorption ionization time of flight mass spectroscopy / Panagiotis G. Zerefos and Antonia Vlahou -- Combining laser capture microdissection and proteomics techniques / Dana Mustafa, Johan M. Kros, and Theo Luider -- Comparison of protein expression by isotope-coded affinity tag labeling / Zhen Xiao and Timothy D. Veenstra -- Analysis of microdissected cells by two-dimensional LC-MS approaches / Chen Li ... [et al.] -- Label-free LC-MS method for the identification of biomarkers / Richard E. Higgs ... [et al.] -- Analysis of the extracellular matrix and secreted vesicle proteomes by mass spectrometry / Zhen Xiao ... [et al.] -- Miniaturized parallelized sandwich immunoassays / Hsin-Yun Hsu, Silke Wittemann, and Thomas O. Joos -- Dissecting cancer serum protein profiles using antibody arrays / Marta Sanchez-Carbayo -- 2D-PAGE maps analysis / Emilio Marengo, Elisa Robotti, and Marco Bobba -- Finding the significant markers : statistical analysis of proteomic data / Sebastien Christian Carpentier ... [et al.] -- Web-based tools for protein classification / Costas D. Paliakasis, Ioannis Michalopoulos, and Sophia Kossida -- Open-source platform for the analysis of liquid chromatography-mass spectrometry (LC-MS) data / Matthew Fitzgibbon ... [et al.] -- Pattern recognition approaches for classifying proteomic mass spectra of biofluids / Ray L. Somorjai.
  • 2009 CRCnetBASE
    edited by Gregory A. Voth.
    This is the first book completely devoted to exploring the state-of-the-art course-graining techniques for molecular simulations. Leading contributors discuss a broad range of topics, including nucleic acids, minimalist models for protein folding, mixed resolution course graining and elastic network models of protein complexes.
  • 2006 Springer
    Per Sunnerhagen, Jure Piškur (eds.).
    Also available: Print – 2006
  • 2011 Atypon
    Ozkan Ufuk Nalbantoglu and Khalid Sayood.
    Recent advances in development of sequencing technology has resulted in a deluge of genomic data. In order to make sense of this data, there is an urgent need for algorithms for data processing and quantitative reasoning. An emerging in silico approach, called computational genomic signatures, addresses this need by representing global species-specific features of genomes using simple mathematical models. This text introduces the general concept of computational genomic signatures, and it reviews some of the DNA sequence models which can be used as computational genomic signatures. The text takes the position that a practical computational genomic signature consists of both a model and a measure for computing the distance or similarity between models. Therefore, a discussion of sequence similarity/distance measurement in the context of computational genomic signatures is presented. The remainder of the text covers various applications of computational genomic signatures in the areas of metagenomics, phylogenetics and the detection of horizontal gene transfer.
  • 2010
    Ankur Dhanik.
    Proteins are biomolecules that play a key role in a wide diversity of vital functions, such as metabolism and signal transmission. Each protein is a linear chain of amino acids that folds into a flexible three-dimensional structure. Protein's flexibility is widely believed to be essential for its function. Motion of a protein occurs at timescales that span several orders of magnitude. Thermal fluctuations, which occur in picoseconds, are small-amplitude, uncorrelated, harmonic motions of the individual atoms. In contrast, conformational deformations closely related to the protein's function occur in microseconds to milliseconds. These slow deformations are usually large-scale, correlated, anharmonic motions that correspond to transitions between meta-stable states, such as binding and non-binding states. In this dissertation we are mainly interested in modeling structural heterogeneity associated with such slow deformations. This dissertation presents new computational methods to study the flexibility of folded protein in the context of three important biological problems: (a) Loop sampling, (b) Interpretation of electron density maps, and (c) Determination of allosteric pathways. Computational modeling of structural heterogeneity in the folded state of a protein is a challenging problem, mainly because of the high-dimensionality of the protein's conformation space and the very small relative volume of its feasible motion space. Although our methods are specific to each of the three problems, they share the same sample and select approach: they combine efficient sampling algorithms that allow us to represent structural heterogeneity in a folded protein by a collection of sampled conformations and selection algorithms that allow us to reliably pick the sampled conformations that provide a solution to the problem. This dissertation demonstrates the power of geometric computation and efficient sampling to model structural heterogeneity in the folded protein.
  • 2015 Springer Protocols
    edited by Peng Zhou, Jian Huang,.
    De novo peptide structure prediction : an overview / Pierre Thévenet [and three others] -- Molecular modeling of peptides / Krzysztof Kuczera -- Improved methods for classification, prediction, and design of antimicrobial peptides / Guangshun Wang -- Building MHC class II epitope predictor using machine learning approaches / Loan Ping Eng, Tin Wee Tan, and Joo Chuan Tong -- Brownian dynamics simulation of peptides with the University of Houston Brownian Dynamics (UHBD) program / Tongye Shen and Chung F. Wong -- Computational prediction of short linear motifs from protein sequences / Richard J. Edwards and Nicolas Palopoli -- Peptide toxicity prediction / Sudheer Gupta [and five others] -- Synthetic and structural routes for the rational conversion of peptides into small molecules / Pasqualina Liana Scognamiglio, Giancarlo Morelli, and Daniela Marasco -- In silico design of antimicrobial peptides / Giuseppe Maccari, Mariagrazia Di Luca, and Riccardo Nifosì -- Information-driven modeling of protein-peptide complexes / Mikael Trellet, Adrien S.J. Melquiond, and Alexandre M.J.J. Bonvin -- Computational approaches to developing short cyclic peptide modulators of protein-protein interactions / Fergal J. Duffy, Marc Devocelle, and Denis C. Shields -- A use of homology modeling and molecular docking methods : to explore binding mechanisms of nonylphenol and bisphenol A with antioxidant enzymes / Mannu Jayakanthan [and three others] -- Computational peptide vaccinology / Johannes Söllner -- Computational modeling of peptide-aptamer binding / Kristen L. Rhinehardt, Ram V. Mohan, and Goundla Srinivas.
  • 2009 Springer Protocols
    edited by Jason McDermott ... [et al.].
    Part 1. Network components -- 1. Identification of cis-regulatory elements in gene co-expression networks using A-GLAM / Leonardo Mariño-Ramírez ... [et al.] -- 2. Structure-based Ab initio prediction of transcription factor-binding sites / L. Angela Liu and Joel S. Bader -- 3. Inferring protein-protein interactions from multiple protein domain combinations / Simon P. Kanaan ... [et al.] -- 4. Prediction of protein-protein interactions: a study of the co-evolution model / Itai Sharon, Jason V. Davis, and Golan Yona -- 5. Computational reconstruction of protein-protein interaction networks: algorithms and issues / Eric Franzosa, Bolan Linghu, and Yu Xia -- 6. Prediction and integration of regulatory and protein-protein interactions / Duangdao Wichadakul, Jason McDermott, and Ram Samudrala -- 7. Detecting hierarchical modularity in biological networks / Erzsebet Ravasz -- Part 2. Network inference -- 8. Methods to reconstruct and compare transcriptional regulatory networks / M. Madan Babu, Benjamin Lang, and L. Aravind -- 9. Learning global models of transcriptional regulatory networks from data / Aviv Madar and Richard Bonneau -- 10. Inferring molecular interactions pathways from eQTL data / Imran Rashid, Jason McDermott, and Ram Samudrala -- 11. Methods for the inference of biological pathways and networks / Roger E. Bumgarner and Ka Yee Yeung -- Part 3. Network dynamics -- 12. Exploring pathways from gene co-expression to network dynamics / Huai Li, Yu Sun, and Ming Zhan -- 13. Network dynamics / Herbert M. Sauro -- 14. Kinetic modeling of biological systems / Haluk Resat, Linda Petzold, and Michel F. Pettigrew -- 15. Guidance for data collection and computational modelling of regulatory networks / Adam Christopher Palmer, and Keith Edward Shearwin -- Part 4. Function and evolutionary systems biology -- 16. Maximum likelihood method for reconstruction of the evolution of eukaryotic gene structure / Liran Carmel, ... [et al.] -- 17. Enzyme function prediction with interpretable models / Umar Syed and Golan Yona -- 18. Using evolutionary information to find specificity-determining and co-evolving residues / Grigory Kolesov and Leonid A. Mirny -- 19. Connecting protein interaction data, mutations, and disease using bioinformatics / Jake Y. Chen, Eunseog Youn, and Sean D. Mooney -- 20. Effects of functional bias on supervised learning of a gene network model / Insuk Lee and Edward M. Marcotte -- Part 5. Computational infrastructure for systems biology -- 21. Comparing algorithms for clustering of expression data: how to assess gene clusters / Golan Yona, William Dirks, and Shafquat Rahman -- 22. Bioverse API and web application / Michal Guerquin ... [et al.] -- 23. Computational representation of biological systems / Zach Frazier ... [et al.] -- 24. Biological network inference and analysis using SEBINI and CABIN / Ronald Taylor and Mudita Singhal.
  • 2012 Springer Protocols
    edited by Michael A.K. Liebschner.
    Computer-aided tissue engineering : benefiting from the control over scaffold micro-architecture / Ahmad M. Tarawneh, Matthew Wettergreen, and Michael A.K. Liebschner -- Computer-aided approach for customized cell-based defect reconstruction / Ulrich Meyer, Jorg Neunzehn, and Hans Peter Wiesmann -- Novel bone scaffold design approach based on shape function and all-hexahedral mesh refinement / Shengyong Cai, Juntong Xi, and Chee Kai Chua -- Rapid prototyping composite and complex scaffolds with PAM2 / Giovanni Vozzi, Annalisa Tirella, and Arti Ahluwalia -- Scaffold pore space modulation through intelligent design of dissolvable microparticles / Michael A.K. Liebschner and Matthew Wettergreen -- Scaffold informatics and biomimetic design : three-dimensional medical reconstruction / Jorge Vicente Lopes da Silva, Tatiana Al-Chueyr Pereira Martins, and Pedro Yoshito Noritomi -- Development of computer-aided system for tissue scaffolds (CASTS) system for functionally graded tissue-engineering scaffolds / Novella Sudarmadji, Chee Kai Chua, and Kah Fai Leong -- Computer-designed nano-fibrous scaffolds / Laura A. Smith and Peter X. Ma -- Neural network technique for remeshing of bone microstructure / Anath Fischer and Yaron Holdstein -- Using the Taguchi method to obtain more finesse to the biodegradable fibers / Ville Ellssa [and others] -- Numerical modeling in the design and evaluation of scaffolds for orthopaedics applications / Wojciech Swieszkowski and Krzysztof J. Kurzydlowski -- Structural and vascular analysis of tissue engineering scaffolds, part 1 : numerical fluid analysis / Henrique A. Almeida and Paulo J. Bartolo -- Structural and vascular analysis of tissue engineering scaffolds, part 2 : topology optimisation / Henrique A. Almeida and Paulo J. Bartolo -- Modeling of bioreactor hydrodynamic environment and its effects on tissue growth / Bahar Bilgen and Gilda A. Barabino -- Recent advances in 3D printing of tissue engineering scaffolds / Min Lee and Benjamin M. Wu -- Graft-artery junctions : design optimization and CAD development / Yos S. Morsi [and others] -- Projection printing of 3-dimensional tissue scaffolds / Yi Lu and Shaochen Chen -- Laser sintering for the fabrication of tissue engineering scaffolds / Stefan Lohfeld and Peter E. McHugh -- Three-dimensional microfabrication by two-photon polymerization technique / Aleksandr Ovsianikov and Boris N. Chichkov -- Direct fabrication as a patient-targeted therapeutic in a clinical environment / Dietmar W. Hutmacher [and others] -- Microstereolithography-based computer-aided manufacturing for tissue engineering / Dong-Woo Cho and Hyun-Wook Kang.
  • 2002 CRCnetBASE
    Pei-Show Juo.
    Also available: Print – 2002
  • 2006 Springer Protocols
    edited by Mary Kearns-Jonker.
    Genetics of cardiac septation defects and their pre-implantation diagnosis / Deborah A. McDermott, Craig T. Basson, and Cathy J. Hatcher -- Molecular and genetic aspects of DiGeorge/velocardiofacial syndrome / Deborah A. Driscoll -- Mutation screening for the genes causing cardiac arrhythmias / Jeffrey A. Towbin -- Mutation analysis of the FBN1 gene in patients with Marfan syndrome / Paul Coucke, Petra Van Acker, and Anne De Paepe -- Mutation analysis of PTPN11 in Noonan syndrome by WAVE / Navaratnam Elanko and Steve Jeffery -- Williams-Beuren syndrome diagnosis using fluorescence in situ hybridization / Lucy R. Osborne, Ann M. Joseph-George, and Stephen W. Scherer -- Congenital heart disease: molecular diagnostics of supravalvular aortic stenosis / May Tassabehji and Zsolt Urban -- "Chip"ping away at heart failure / J. David Barrans and Choong-Chin Liew -- Molecular diagnostics of catecholaminergic polymorphic ventricular tachycardia using denaturing high-perfomance liquid chromatography and sequencing / Alex V. Postma, Zahurul A. Bhuiyan, and Hennie Bikker -- Mutation detection in tumor suppressor genes using archival tissue specimens / Aristotelis Astrinidis and Elizabeth Petri Henske -- Friedreich ataxia: detection of GAA repeat expansions and frataxin point mutations / Massimo Pandolfo -- The cardiovascular manifestations of Alagille syndrome and JAG1 mutations / Elizabeth Goldmuntz, Elizabeth Moore, and Nancy B. Spinner -- Array analysis applied to malformed hearts: molecular dissection of tetralogy of Fallot / Silke Sperling -- DNA mutation analysis in heterotaxy / Stephanie M. Ware -- Use of denaturing high-performance liquid chromatography to detect mutations in pediatric cardiomyopathies / Amy J. Sehnert.
  • 2013 Springer Protocols
    edited by Bernice Wright, Che J. Connon.
    Continuous regeneration of the cornea is necessary to maintain this tissue in the transparent state that is essential for vision. Therapy for repair of the damaged anterior cornea is currently addressed through the transplantation of donor corneas or the delivery of limbal epithelial stem cells (LESC) to the ocular surface using amniotic membrane (AM) as a supporting scaffold. Research on the bioengineering of corneal equivalents as replacement tissue is underway to develop viable corneal prosthetics. Corneal Regenerative Medicine: Methods and Protocols provides a concise overview of essential techniques in the field of corneal regenerative medicine, highlighting novel strategies to guide the management of key therapies within this area of medicine. Divided into four convenient sections, topics include the identification, characterisation and cultivation of LESC, as well as the investigation of biopolymers used as the basis for corneal substitutes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Corneal Regenerative Medicine: Methods and Protocols covers the fundamental techniques useful for both the laboratory and clinical settings.
  • 2013 Springer
    Rodolphe Barrangou, John van der Oost, editors.
    CRISPR-Cas is a recently discovered defense system which protects bacteria and archaea against invasion by mobile genetic elements such as viruses and plasmids. A wide spectrum of distinct CRISPR-Cas immune systems has been identified in at least half of the available prokaryotic genomes. On-going biochemical and functional analyses have resulted in substantial insight into the functions and possible applications of these fascinating systems, although many secrets remain to be uncovered. In this book, experts summarize the state of the art of this exciting field.
  • 2007 Springer Protocols
    edited by John G. Day, Glyn N. Stacey.
  • 2015 Springer Protocols
    edited by Willem F. Wolkers, Harriette Oldenhof.
    This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.
  • 1997- Wiley
    editorial board, J. Paul Robinson, managing editor ... [et al.].
    Consists of a core publication dated Apr. 1997, and updated by supplements 1 (July 1997)-<57 (July 2010)>. Content is peer-reviewed protocols for flow and image cytometry updated quarterly.
  • 2002- Wiley
    edited by Frederick M. Ausubel ... [et al.].
    ch. 1. Escherichia coli, Plasmids, and Bacteriophages -- ch. 2. Preparation and Analysis of DNA -- ch. 3. Enzymatic Manipulation of DNA and RNA -- ch. 4. Preparation and Analysis of RNA -- ch. 5. Construction of Recombinant DNA Libraries -- ch. 6. Screening of Recombinant DNA Libraries -- ch. 7. DNA Sequencing -- ch. 8. Mutagenesis of Cloned DNA -- ch. 9. Introduction of DNA into Mammalian Cells -- ch. 10. Analysis of Proteins -- ch. 11. Immunology -- ch. 12. DNA-Protein Interactions -- ch. 13. Yeast -- ch. 14. In Situ Hybridization and Immunohistochemistry -- ch. 15. The Polymerase Chain Reaction -- ch. 16. Protein Expression -- ch. 17. Preparation and Analysis of Glycoconjugates -- ch. 18. Analysis of Protein Phosphorylation -- ch. 19. Informatics for Molecular Biologists -- ch. 20. Analysis of Protein Interactions -- ch. 21. Chromatin Assembly and Analysis -- ch. 22. Nucleic Acid Arrays -- ch. 23. Manipulating the Mouse Genome -- ch. 24. Generation and Use of Combinatorial Libraries -- ch. 25. Discovery and Analysis of Differentially Expressed Genes in Single Cells and Cell Populations -- ch. 26. Gene Silencing -- ch. 27. RNA-Protein Interactions -- ch. 28. Mammalian Cell Culture -- ch. 29. Mouse Phenotyping -- ch. 30. Metabolomics -- Appendix 1. Standard Measurements, Data, and Abbreviations -- Appendix 2. Commonly Used Reagents and Equipment -- Appendix 3. Commonly Used Techniques in Biochemistry and Molecular Biology -- Appendix 4. Suppliers -- Appendix 5. Vectors -- Appendix 6. Pathway Modulators and Inhibitors.
  • [1995]- Wiley
    editorial board, John E. Coligan ... [et al.].
    Also available: Print – 1995-2005.
  • 2013 Springer Protocols
    edited by Chris Gehring.
    Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better understand the biological role of this important signaling system. Written in the highly successful Methods in Molecular Biology [trademark] series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cyclic Nucleotide Signaling in Plants: Methods and Protocols seeks to aid scientist in further understanding signal transduction and the molecular mechanisms underlying cellular signaling.
  • 2013 Springer Protocols
    edited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano.
    This volume provides a wide range of techniques accessible to researchers in biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics. It features step-by-step, readily reproducible protocols and notes on troubleshooting.
  • 2011 Springer Protocols
    edited by Michael Hamacher, Martin Eisenacher and Christian Stephan.
    Instruments and methods in proteomics -- In-depth protein characterization by mass spectrometry -- Analysis of phosphoproteomics data -- The origin and early reception of sequence databases -- Laboratory data and sample management for proteomics -- PRIDE and "Database on demand" As valuable tools for computational proteomics -- Analysing proteomics identifications in the context of functional and structural protein annotation: Integrating annotation using PICR, DAS, and BioMart -- Tranche distributed repository and proteomecommons.Org -- Data standardization by the HUPO-PSI: How has the community benefitted? -- mzIdentML: An open community-built standard format for the results of proteomics spectrum identification algorithms -- Spectra, chromatograms, metadata: mzML-the standard data format for mass spectrometer output -- imzML: Imaging mass spectrometry markup language: A common data format for mass spectrometry imaging -- Tandem mass spectrometry spectral libraries and library searching -- Inter-lab proteomics: Data mining in collaborative projects on the basis of the HUPO brain proteome project's pilot studies -- Data management and data integration in the HUPO plasma proteome project -- Statistics in experimental design, preprocessing, and analysis of proteomics data -- The evolution of protein interaction networks -- Cytoscape: Software for visualization and analysis of biological networks -- Text mining for systems modeling -- Identification of alternatively spliced transcripts using a proteomic informatics approach -- Distributions of ion series in ETD and CID spectra: Making a comparison -- Evaluation of peak-picking algorithms for protein mass spectrometry -- OpenMS and TOPP: Open source software for LC-MS data analysis -- LC/MS data processing for label-free quantitative analysis -- Spectral properties of correlation matrices - towards enhanced spectral clustering -- Standards, databases, and modeling tools in systems biology -- Modeling of cellular processes: Methods, data, and requirements.
  • 2012 Springer Protocols
    edited by François Pompanon and Aurélie Bonin.
    Sampling and experimental design: Sampling in landscape genomics / Stéphanie Manel, Cécile H. Albert, and Nigel G. Yoccoz. OligoTag : a program for designing sets of tags for next-generation sequencing of multiplexed samples / Eric Coissac. SNP discovery in non-model organisms using 454 next generation sequencing / Christopher W. Wheat. In silico fingerprinting (ISIF) : a user-friendly in silico AFLP program / Margot Paris and Laurence Després -- Producing data: Diversity arrays technology : a generic genome profiling technology on open platforms / Andrzej Kilian [and others]. Two methods to easily obtain nucleotide sequences from AFLP loci of interest / Margot Paris [and others]. Roche genome sequencer flx based high-throughput sequencing of ancient DNA / David E. Alquezar-Planas and Sarah L. Fordyce. Preparation of normalized cDNA libraries for 454 titanium transcriptome sequencing / Zhao Lai [and others]. RAD paired-end sequencing for local de novo assembly and SNP discovery in non-model organisms / Paul D. Etter and Eric Johnson -- Analyzing data: Automated scoring of AFLPs using RawGeno v 2.0, a free R CRAN library / Nils Arrigo, Rolf Holderegger, and Nadir Alvarez. Haplotype inference / Olivier Delaneau and Jean-François Zagury-- Allele identification in assembled genomic sequence datasets / Katrina M. Dlugosch and Aurélie Bonin. Multiple testing in large-scale genetic studies / Matthieu Bouaziz, MArine Jeanmougin, and Mickaël Guedj. Population genomic analysis of model and nonmodel organisms using sequenced RAD tags / Paul A. Hobenlobe, Julian Catchen, and William A. Cresko. Analysis and management of gene and allelic diversity in subdivided populations using the software program METAPOP / Andrés Pérez-Figueroa, Silvia T. Rodriquez-Ramillo, and ARmando Caballero. DetSel : an R-package to detect marker loci responding to selection / Renaud Vitalis. Use of qualitative environmental and phenotypic variables in the context of allele distribution models : detecting signatures of selection in the genome of Lake Victoria cichlids / Stéphane Joost [and others]. Genomic scan as a tool for assessing the genetic component of phenotypic variance in wild populations / Carlos M. Herrera.
  • 2005 Springer
    Alvaro Macieira-Coelho (ed.).
    The hedgehog signaling pathway in cancer / J. Vestergaard, Mads Bak, L.A. Larsen -- Rho GTPases and cancer / P. Buongiorno, B. Bapat -- Developmental biology of fibroblasts and neoplastic disease / D. Brouty-Boyé -- The extracellular matrix during normal development and neoplastic growth / J. Labat-Robert, L. Robert -- Wilms' tumor : starting off the kidney all over again? / M. Little -- Testicular teratomas : back to pluripotent stem cells / T. Kimura ... [et al.] / Tumor suppressors APC and VHL : gatekeepers of the intestine and kidney / R.H. Giles, E.E. Voest -- Hormonal and stromal regulation of normal and neoplastic prostatic growth / W.A. Ricke ... [et al.] -- Neoplastic growth through the developmental stages of the organism / A. Maciera-Coelho.
    Also available: Print – 2005
  • 2013 Springer
    [edited by] Annikka Weissferdt, Cesar A. Moran.
    Diagnostic Imaging of Lung and Pleural Tumors -- The Staging of Lung Cancer -- Non-Small Cell Carcinomas -- Neuroendocrine Carcinomas -- Biphasic Tumors of the Lungs -- Salivary Gland and Adnexal Type Tumors of the Lungs -- Tumors Derived from Presumed Ectopic Tissues -- Vascular Tumors of the Lungs -- Mesenchymal Tumors of the Lungs -- Lung Tumors of Uncertain Histogenesis -- Malignant Lymphomas Involving Lung and Pleura -- Tumors of the Pleura -- Tumor-like Conditions and Benign Tumors of the Lung -- Molecular Pathology of Lung Cancer.
  • Knovel
    J. Stenesh.
    Also available: Print – 1989
  • 2006
    Paul Singleton, Diana Sainsbury.
    Status: Not Checked OutLane Catalog Record
  • 2005 Springer Protocols
    edited by Peng Liang, Jonathan D. Meade, Arthur B. Pardee.
    Global analysis of gene expression by differential display : a mathematical model / Shitao Yang and Peng Liang -- Automation of fluorescent differential display with digital readout / Jonathan D. Meade ... [et al.] -- Ordered differential display / Mikhail V. Matz and Ella A. Meleshkevitch -- GeneCalling : transcript profiling coupled to a gene database query / Richard A. Shimkets -- High-density sampling differential display of prokaryotic mRNAs with RAP-PCR / Dana M. Walters and Pierre E. Rouviere -- Vertical arrays : microarrays of complex mixtures of nucleic acids / Rosana Risques ... [et al.] -- Automated pattern ranking in differential display data analysis / Tero Aittokallio ... [et al.] -- Linking cDNA-AFLP-based gene expression patterns and ESTs / Ling Qin, Pjotr Prins, and Johannes Helder -- Differentially expressed genes associated with hepatitis B virus HBx and MHBs protein function in hepatocellular carcinoma / Dae-Ghon Kim -- Identification of disease markers by differential display : prion disease / Michael Clinton ... [et al.] -- Saturation screening for p53 target genes by digital fluorescent differential display / Yong-Jig Cho ... [et al.] -- Identification of p53-regulated genes by the method of differential display / Yunping Lin, Roger P. Leng, and Samuel Benchimol -- Identification by differential display of IL-24 autocrine loop activated by ras oncogenes / Zhongjia Tan, Mai Wang, and Peng Liang -- Comprehensive analysis of ovarian gene expression during ovulation using differential display / Lawrence L. Espey -- Functional analysis of nocturnin : a circadian clock-regulated gene identified by differential display / Julie E. Baggs and Carla B. Green -- Isolation and characterization of anthocyanin 5-0-glucosyltransferase in perilla frutescens var. crispa by differential display / Mami Yamasaki and Kazuki Saito -- Identification of target genes of a yeast transcriptional repressor / Bernard Mai and Linda L. Breeden -- Detection of an mRNA polymorphism by differential display / Shan Liang ... [et al.] -- Silencing in yeast : identification of CLr4 targets / Sergey V. Ivanov and Alla V. Ivanova -- Identification of mRNA bound to RNA binding proteins by differential display / Anne Carr-Schmid, Xinfu Jiao, and Megerditch Kiledjian.
  • 2007 CRCnetBASE
    Krishnendu Chakrabarty, Fei Su.
  • 2014 Springer Protocols
    edited by Elizabeth M.J. Gillam, School of Chemistry and Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia, Janine N. Copp, Michael Smith Laboratories, University of British Columbia, Vancouver, BC, Canada, David F. Ackerley, School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand.
  • 2006 Springer
    [edited by] Frank Eisenhaber.
    Bioinformatics: mystery, astrology, or service technology? / Frank Eisenhaber -- Reliable and specific protein function prediction by combining homology with genomic(s) context / Martijn A. Huynen, Berend Snel, and Toni Gabalden -- Clues from three-dimensional structure analysis and molecular modelling: new insights into cytochrome P450 mechanisms and functions / Karin Schleinkofer and Thomas Dandekar -- Prediction of protein function: two basic concepts and one practical recipe / Frank Eisenhaber -- Extracting information for meaningful function inference through text-mining / Hong Pan ... [et al.] -- Literature and genome data mining for prioritizing disease-associated genes / Carolina Perez-Iratxeta, Peer Bork, and Miguel A. Andrade -- Model-based inference of transcriptional regulatory mechanisms from DNA microarray data / Harmen J. Bussemaker -- The predictive power of molecular network modelling: case studies of predictions with subsequent experimental verification / Stefan Schuster, Edda Klipp, and Marko Marhl -- Theory of early molecular evolution: predictions and confirmations / Edward N. Trifonov -- Hitchhiking mapping: limitations and potential for the identification of ecologically important genes / Christian Schlötterer -- Understanding the functional importance of human single nucleotide polymorphisms / Saurabh Asthana and Shamil Sunyaev -- Correlations between quantitative measures of genome evolution, expression, and function / Yuri I. Wolf, Liran Carmel, and Eugene V. Koonin.
  • 2005 Springer
    Michael R. Emmert-Buck, John W. Gillespie, Rodrigo F. Chuaqui, (eds.).
  • 2012 Springer Protocols
    edited by W. John Kress and David L. Erickson.
    DNA barcodes : methods and protocols / W. John Kress and David L. Erickson -- Introduction to animal DNA barcoding protocols / Lee A. Weigt [and others] -- DNA barcodes for insects / John James Wilson -- DNA barcoding methods for invertebrates / Nathaniel Evans and Gustav Paulay -- DNA barcoding amphibians and reptiles / Miguel Vences [and others] -- DNA barcoding fishes / Lee A. Weigt -- DNA barcoding birds : from field collection to data analysis / Dario A. Lijtmaer [and others] -- DNA barcoding in mammals / Natalia V. Ivanova, Elizabeth L. Clare, and Alex V. Borisenko -- Methods for DNA barcoding of fungi / Ursula Eberhardt -- Methods for DNA barcoding photosynthetic protists emphasizing the macroalgae and diatoms / Gary W. Saunders and Daniel C. McDevit -- DNA barcoding methods for land plants / Aron J. Fazekas [and others] -- Field information management systems for DNA barcoding / John Deck [and others] -- Laboratory information management systems for DNA barcoding / Meaghan Parker [and others] -- DNA extraction, preservation, and amplification / Thomas Knebelsberger and Isabella Stoger -- DNA mini-barcodes / Mehrdad Hajibabaei and Charly McKenna -- Ways to mix multiple PCR amplicons into single 454 run for DNA barcoding / Ryuji J. Machida and Nancy Knowlton -- Practical evaluation of DNA barcode efficacy / John L. Spouge and Leonardo Marino-Ramirez -- Plant DNA Barcodes, Taxonomic Management and Species Discovery in Tropical Forests / Christopher W. Dick and Campbell O. Webb -- Construction and Analysis of Phylogenetic Trees Using DNA Barcode Data / David L. Erickson and Amy C. Driskell -- Phylogenetic analyses of ecological communities using DNA barcode data / Nathan G. Swenson -- FISH-BOL, a case study for DNA barcodes / Robert D. Ward -- Generating plant DNA barcodes for trees in long-term forest dynamics plots / W. John Kress, Ida C. Lopez, and David L. Erickson -- Future directions / David L. Erickson and W. John Kress.
  • 2014 Springer Protocols
    edited by Svein Valla and Rahmi Lale.
    1. BioBrick assembly standards and techniques and associated software tools / Gunvor Røkke, Eirin Korvald, Jarle Pahr, Ove Øyås and Rahmi Lale -- 2. Plasmid construction by SLIC or sequence and ligation- independent cloning / Ryan E. Hill and Julian J. Eaton-Rye -- 3. Quick and clean cloning / Frank Thieme and Sylvestre Marillonnet -- 4. Hierarchical ligation-independent assembly of PCR fragments / Jonathan L. Schmid-Burgk, Zhen Xie, and Yaakov Benenson -- 5. USER-derived cloning methods and their primer design / Bo Salomonsen, Uffe H. Mortensen, and Barbara A. Halkier -- 6. Application of the restriction-free (RF) cloning for multicomponents assembly / Yoav Peleg and Tamar Unger -- 7. A single-tube assembly of DNA using the transfer-PCR (TPCR) platform / Ariel Erijman, Julia M. Shifman, and Yoav Peleg -- 8. Circular polymerase extension cloning / Jiayuan Quan and Jingdong Tian -- 9. Golden gate cloning / Carola Engler and Sylvestre Marillonnet -- 10. Design and construction of multigenic constructs for plant biotechnology using the goldenbraid cloning strategy / Alejandro Sarrion-Perdigones, Jorge Palaci, Antonio Granell and Diego Orzaez -- 11. FX cloning : a simple and robust high-throughput cloning method for protein expression / Eric R. Geertsma -- 12. Minimum GC-rich sequences for overlap extension PCR and primer annealing / Mikiko Nakamura, Ayako Suzuki, Hisashi Hoshida and Rinji Akada -- 13. Simple cloning and DNA assembly in Escherichia coli by prolonged overlap extension PCR / Chun You and Y.-H. Percival Zhang -- 14. Combinatorial assembly of clone libraries using site-specific recombination / Vanessa E. Wall, Leslie A. Garvey, Jennifer L. Mehalko, Lauren V. Procter and Dominic Esposito -- 15. Application of In-Fusion [trademark] cloning for the parallel construction of E. coli expression vectors / Louise E. Bird, Heather Rada, John Flanagan, Jonathan M. Diprose, Robert J.C. Gilbert and Raymond J. Owens -- 16. Seamless ligation cloning extract (SLiCE) cloning method / Yongwei Zhang, Uwe Werling, and Winfried Edelmann -- 17. j5 DNA assembly design automation / Nathan J. Hillson -- 18. FastPCR software for PCR, in silico PCR, and oligonucleotide assembly and analysis / Ruslan Kalendar, David Lee, and Alan H. Schulman.
  • 2009 Springer Protocols
    edited by Vladimir V. Didenko.
    In situ detection of apoptosis by the TUNEL assay: An overview of techniques -- Combination of TUNEL assay with immunohistochemistry for simultaneous detection of DNA fragmentation and oxidative cell damage -- EM-ISEL: A useful tool to visualize DNA damage at the ultrastructural level -- In situ labeling of DNA breaks and apoptosis by T7 DNA polymerase -- In situ ligation: A decade and a half of experience -- In situ ligation simplified: Using PCR fragments for detection of double-strand DNA breaks in tissue sections -- 5'OH DNA breaks in apoptosis and their labeling by topoisomerase-based approach -- Detection of DNA strand breaks in apoptotic cells by flow- and image-cytometry -- Fluorochrome-labeled inhibitors of caspases: Convenient in vitro and in vivo markers of apoptotic cells for cytometric analysis -- Combining fluorescent in situ hybridization with the comet assay for targeted examination of DNA damage and repair -- Simultaneous labeling of single- and double-strand DNA breaks by DNA breakage detection-FISH (DBD-FISH) -- Co-localization of DNA repair proteins with UV-induced DNA damage in locally irradiated cells -- Ultrasound imaging of apoptosis: Spectroscopic detection of DNA-damage effects at high and low frequencies -- Quantifying etheno-DNA adducts in human tissues, white blood cells, and urine by ultrasensitive (32)P-postlabeling and immunohistochemistry -- ELISpot assay as a tool to study oxidative stress in peripheral blood mononuclear cells -- Cytokinesis-block micronucleus cytome assay in lymphocytes -- Buccal micronucleus cytome assay -- [Gamma]-H2AX detection in peripheral blood lymphocytes, splenocytes, bone marrow, xenografts, and skin -- Immunologic detection of benzo(a)pyrene-DNA adducts -- Non-invasive assessment of oxidatively damaged DNA: Liquid chromatography-tandem mass spectrometry analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine -- Assessing sperm DNA fragmentation with the sperm chromatin dispersion test.
  • 2012 Springer Protocols
    edited by Antonio Alonso.
    An overview of DNA typing methods for human identification : past, present, and future / Robyn Thompson, Silvia Zoppis, and Bruce McCord -- Capillary electrophoresis and 5-channel LIF detection of a 26plex autosomal STR asay for human identification / Carolyn R. Hill -- Capillary electrophoresis of miniSTR markers to genotype highly degraded DNA samples / Michael D. Coble -- Interpretation guidelines of a standard Y-chromosome STR 17-plex PCR-CE assay for crime casework / Lutz Roewer and Maria Geppert -- Capillary electrophoresis of an X-chromosome STR decaplex for kinship deficiency cases / Leonor Gusmão [and others] -- A 48-plex autosomal SNP GenPlex Assay for human individualization and relationship testing / Carmen Tomas, Claus Børsting, and Niels Morling -- Typing of 49 autosomal SNPs by single base extension and capillary electrophoresis for forensic genetic testing / Claus Børsting, Carmen Tomas, and Niels Morling -- A 34-plex autosomal SNP single base extension assay for ancestry investigations / C. Phillips, M. Fondevila, and Maria Victoria Lareau -- SNaPshot® minisequencing analysis of multiple ancestry-informative Y-SNPs using capillary electrophoresis / Maria Geppert and Lutz Roewer -- Capillary electrophoresis of 38 noncoding biallelic mini-indels for degraded samples and as complementary tool in paternity testing / Rui Pereira and Leonor Gusmão -- Capillary electrophoresis of an 11-plex mtDNA coding region SNP single base extension assay for discrimination of themost common caucasian HV1/HV2 mitotype / Peter M. Vallone -- Capillary electrophoresis of a multiplex reverse transcription-polymerase chain reaction to target messenger RNA markers for body fluid identification / Cordula Haas, Erin Hanson, and Jack Ballantyne -- DNA extraction from aged skeletal samples for STR typing by capillary electrophoresis / René Huel [and others] -- Interpretation guidelines for multilocus STR forensic profiles from low template DNA samples / Zoran M. Budimlija and Theresa A. Caragine -- Interpretation guidelines for mixed-STR multilocus electrophoretic profiles / Juan Antonio Luque -- Capillary electrophoresis analysis of a 9-plex STR assay for canine genotyping / Barbara van Asch and António Amorim -- Capillary electrophoresis of DNA from Cannabis sativa for correlation of samples to geographic origin / Heather Mller Coyle -- Capillary electrophoresis of multigene barcoding chloroplast markers for species identification of botanical trace evidence / Gianmarco Ferri, Beatrice Corradini, and Milena Alù -- Capillary electrophoresis of big-dye terminator sequencing reactions for human mtDNA control region haplotyping in the identification of human remains / Marta Montesino and Lourdes Prieto -- Capillary electrophoresis of human mtDNA control region sequences from highly degraded samples using short mtDNA amplicons / Odile M. Loreille and Jodi A. Irwin -- Interpretation guidelines of mt DNA control region sequence electropherograms in forensic gnetics / Manuel Crespillo Marquez -- Capillary electrophoresis of mtDNA cytochrome b gene sequences for animal species identification / Adrian Linacre -- Sequence analysis of the canine mitochondrial DNA control region from shed hair samples in criminal investigations / C. Berger, B. Berger, and W. Parson -- Integrated sample cleanup and microchip capillary array electrophoresis for high-performance forensic STR profiling / Peng Liu [and others] -- Microchip capillary electrophoresis protocol to evaluate quality and quantity of mtDNA amplified fragments for DNA sequencing in forensic genetics / Coro Fernández and Antonio Alonso -- Microchip capillary electrophoresis of multi-locus VNTR analysis for genotyping of Bacillus anthracis and Yersinia pestis in microbial forensic cases / Andrea Ciammaruconi.
  • 2013 Springer
    Maria Spies, editor.
    Also available: Print – 2013
  • 2015 Springer Protocols
    edited by Benoit P. Leblanc and Sebastien Rodrigue.
    Electrophoretic mobility shift assay using radiolabeled DNA probes -- In vitro DNase i footprinting -- Determining the architecture of a protein-DNA complex by combining FeBABE cleavage analyses, 3-D printed structures, and the ICM molsoft program -- In cellulo DNA analysis: LMPCR footprinting -- Southwestern blotting assay -- Single-molecule approaches for the characterization of riboswitch folding mechanisms -- Probing of nascent riboswitch transcripts -- Functional studies of DNA-protein interactions using fret techniques -- Precise identification of genome-wide transcription start sites in bacteria by 5'-rapid amplification of cDNA ends (5'-RACE) -- Analysis of DNA supercoiling induced by DNA-protein interactions -- Precise identification of DNA-binding proteins genomic location by exonuclease coupled chromatin immunoprecipitation (ChIP-exo) -- The cruciform DNA mobility shift assay: A tool to study proteins that recognize bent DNA -- Individual and sequential chromatin immunoprecipitation protocols -- Chromatin endogenous cleavage (ChEC) as a method to quantify protein interaction with genomic DNA in saccharomyces cerevisiae -- Selection and validation of spacer sequences for CRISPR-Cas9 genome editing and transcription regulation in bacteria -- Detection of short-range DNA interactions in mammalian cells using high-resolution circular chromosome conformation capture coupled to deep sequencing -- Global mapping of open chromatin regulatory elements by formaldehyde-assisted isolation of regulatory elements followed by sequencing (FAIRE-seq) -- Aggregate and heatmap representations of genome-wide localization data using VAP, a versatile aggregate profiler -- Circular dichroism for the analysis of protein-DNA interactions -- Quantitative investigation of protein-nucleic acid interactions by biosensor surface plasmon resonance -- Identification of nucleic acid high affinity binding sequences of proteins by SELEX.
  • 2009 Springer Protocols
    edited by Tom Moss and Benoît Leblanc.
    Filter-binding assays / Peter G. Stockley -- Electrophoretic mobility shift assays for the analysis of DNA-protein interactions / Manon Gaudreault ... [et al.] -- DNase I footprinting / Benoît Leblanc and Tom Moss -- Exonuclease III footprinting on immobilized DNA templates / Patrizia Spitalny and Michael Thomm -- Hydroxyl radical footprinting of protein-DNA complexes / Indu Jagannathan and Jeffrey J. Hayes -- The use of diethyl pyrocarbonate and potassium permanganate as probes for strand separation and structural distortions in DNA / Brenda F. Kahl and Marvin R. Paule -- Uranyl photofootprinting / Peter E. Nielsen -- Identification of protein/DNA contacts with dimethyl sulfate : methylation protection and methylation interference / Peter E. Shaw and A. Francis Stewart -- Ethylation interference footprinting of DNA-protein complexes / Iain W. Manfield and Peter G. Stockley -- Site-directed cleavage of DNA by protein-Fe(II) EDTA conjugates within model chromatin complexes / David R. Chafin and Jeffrey J. Hayes -- Identification of nucleic acid high-affinity binding sequences of proteins by SELEX / Philippe Bouvet -- Identification of sequence-specific DNA-binding proteins by southwestern blotting / Simon Labbé, Jean-François Harrisson, and Carl Séguin -- Footprinting DNA-protein interactions in native polyacrylamide gels by chemical nucleolytic activity of 1,10-phenanthroline-copper / Athanasios G. Papavassiliou -- Determination of a transcription factor-binding site by nuclease protection footprinting onto southwestern blots / Athanasios G. Papavassiliou -- Use of a reporter gene assay in yeast for genetic analysis of DNA-protein interactions / David R. Setzer ... [et al.] -- Chromatin immunoprecipitation in mammalian cells / Amy Svotelis, Nicolas Gévry, and Luc Gaudreau -- Sequential chromatin immunoprecipitation protocol : ChIP-reChIP / Mayra Furlan-Magaril, Héctor Rincón-Arano, and Félix Recillas-Targa -- Profiling genome-wide histone modifications and variants by ChIP-chip on tiling microarrays in S. cerevisiae / Alain R. Bataille and François Robert -- Nucleosome mapping / Nicolas Gévry ... [et al.] -- In cellulo DNA analysis (LMPCR footprinting) / Régen Drouin ... [et al.] -- Atomic force microscopy imaging and probing of DNA, proteins, and protein DNA complexes : silatrane surface chemistry / Yuri L. Lyubchenko, Luda S. Shlyakhtenko, and Alexander A. Gall -- Two-dimensional crystallisation of soluble protein complexes / Patrick Schultz, Corinne Crucifix, and Luc Lebeau -- Assays for transcription factor activity / Douglas Browning ... [et al.] -- Ultraviolet crosslinking of DNA protein complexes via 8-azidoadenine / Rainer Meffert ... [et al.] -- Static and kinetic site-specific protein-DNA photocrosslinking : analysis of bacterial transcription initiation complexes / Nikolai Naryshkin ... [et al.] -- Use of site-specific protein-DNA photocrosslinking of purified complexes to analyze the topology of the RNA polymerase II transcription initiation complex / Diane Forget, Céline Domecq, and Benoit Coulombe -- Site-directed DNA crosslinking of large multisubunit protein-DNA complexes / Jim Persinger and Blaine Bartholomew -- Functional studies of DNA-protein interactions using FRET techniques / Simon Blouin ... [et al.] -- Single-molecule FRET analysis of protein-DNA complexes / Mike Heilemann ... [et al.] -- Analysis of DNA supercoiling induced by DNA-protein interactions / David J. Clark and Benoît Leblanc -- The cruciform DNA mobility shift assay : a tool to study proteins that recognize bent DNA / Victor Y. Stefanovsky and Tom Moss -- Plasmid vectors for the analysis of protein-induced DNA bending / Christian Zwieb and Sankar Adhya -- Analysis of distant communication on defined chromatin templates in vitro / Yury S. Polikanov and Vasily M. Studitsky -- A competition assay for DNA binding using the fluorescent probe ANS / Ian A. Taylor and G. Geoff Kneale -- Fluorescence spectroscopy and anisotropy in the analysis of DNA-protein interactions / Rosy Favicchio ... [et al.] -- Circular dichroism for the analysis of protein-DNA interactions / P.D. Cary and G. Geoff Kneale -- Defining the thermodynamics of protein/DNA complexes and their components using micro-calorimetry / Colyn Crane-Robinson, Anatoly I. Dragan, and Christopher M. Read -- Surface plasmon resonance assays of DNA-protein interactions / Peter G. Stockley and Björn Persson.
  • 2008 Springer Protocols
    edited by Christian Dahmann.
    Drosophila melanogaster and the development of biology in the 20th century / Alfonso Martinez Arias -- Getting started : an overview on raising and handling Drosophila / Hugo Stocker and Peter Gallant -- FlyBase : a database for the Drosophila research community / Rachel Drysdale and the FlyBase Consortium -- The use of P-element transposons to generate transgenic flies / André Bachmann and Elisabeth Knust -- The GAL4 system : a verstile system for the expression of genes / David A. Elliott and Andrea H. Brand -- P-element mutagenesis / Thomas Hummel and Christian Klämbt -- EMS screens : from mutagenesis to screening and mapping / Christian Bökel -- RNAi screening in cultured Drosophila cells / Sandra Steinbrink and Michael Boutros -- Methods for homologous recombination in Drosophila / Keith A. Maggert, Wei J. Gong, and Kent G. Golic -- Recombinases and their use in gene activation, gene inactivation, and transgenesis / Johannes Bischof and Konrad Basler -- Cuticle preparation of Drosophila embryos and larvae / Cyrille Alexandre -- Immunolabeling of embryos / H.-Arno J. Müller -- Imaging cellular and molecular dynamics in live embryos using fluorescent proteins / Matthieu Cavey and Thomas Lecuit -- Analysis of neuromuscular junctions : histology and in vivo imaging / Andreas Schmid and Stephan J. Sigrist -- Immunolabeling of imaginal discs / Thomas Klein -- Imaging Drosophila pupal wing morphogenesis / Anne-Kathrin Classen ... [et al.] -- Microscopic analysis of the adult Drosophila retina using semithin plastic section / Konstantin Gaengel and Marek Mlodzik -- Fluorescent in situ hybridization protocols in Drosophila embryos and tissues / Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause -- Linear RNA amplification for the production of microarray hybridization probes / Ansgar Klebes and Thomas B. Kornberg -- An overview of the identification, detection, and functional analysis of Drosophila microRNAs / Nicholas S. Sokol -- Extraction and immunoblotting of proteins from embryos / Andreas Wodarz -- Purification of Drosophila protein complexes for mass spectrometry / Christoph Jüschke and Jürgen A. Knoblich -- Mass production of Drosophila embryos and chromatographic purification of native protein complexes / Natascha Kunert and Alexander Brehm -- Flow cytometric analysis of Drosophila cells / Aida Flor A. de la Cruz and Bruce A. Edgar -- Drosophila cell lines as model systems and as an experimental tool / Buzz Baum and Lucy Cherbas -- Appendix : phenotypic markers in Drosophila / Jennifer Childress and Georg Halder.
  • 2011 Springer Protocols
    edited by Seetharama D. Satyanarayanajois.
    Virtual screening for lead discovery / Yat T. Tang and Garland R. Marshall -- Computer-aided drug discovery and development / Shuxing Zhang -- Using active site mapping and receptor-based pharmacophore tools : prelude to docking and de novo/fragment-based ligand design / Ashutosh Tripathi, J. Andrew Surface, and Glen E. Kellogg -- Methods for evaluation of structural and biological properties of antiinvasive natural products / Mudit Mudit [and others] -- Solid-phase peptide synthesis using microwave irradiation / Justin K. Murray, Jennifer Aral, and Les P. Miranda -- Fluorescent and lanthanide labeling for ligand screens, assays, and imaging / Jatinder S. Josan [and others] -- DNA-directed assembly microarray for protein and small molecule inhibitor screening / Ng Jin Kiat [and others] -- Selection of peptide ligands for human placental transcytosis systems using in vitro phage display / Saleem Basha, Shruthi Vaidhyanathan, and GIovanni M. Pauletti -- Optimization of the tetrazolium dye (MTT) colorimetric assay for cellular growth and viability / Paul W. Sylvester -- Imaging NF-KB signaling in mice for screening anticancer drugs / Delira Robbins and Yunfeng Zhao -- Evaluation of anticancer agents using flow cytometry analysis of cancer stem cells / Vineet Gupta, Qian-Jin Zhang, and Yong-Yu Liu -- Chemical screening with zebrafish embryos / Hanbing Zhong and Shuo Lin -- iTRAQ labeling coupled with LC-MALDI mass spectrometry for monitoring temporal response of colorectal cancer cells to butyrate treatment / Hwee Tong Tan [and others] -- Heterotypic cell adhesion assay for the study of cell adhesion inhibition / Seetharama D. Satyanarayanajois, Sharon Ronald, and Jining Liu -- Evaluation of antibacterial activity of proteins and peptides using a specific animal model for wound healing / Ramar Perumal Samy [and others] -- In vitro immunogenicity risk assessment of therapeutic proteins in preclinical setting / Punett Gaitonde and Sathy V. Balu-Iyer.
  • ScienceDirect
    edited by Jonathan B. Chaires, Michael J. Waring.
    Biophysical approaches -- Chemical and molecular biological approaches -- Enzymology and biological approaches.
    Also available: Print – 2001
  • 2005 Springer
    Valeria Matranga, (ed.).
    Are echinoderms of interest to biotechnology? / C. Petzelt -- Cell adhesion and communication : a lesson from echinoderm embryos for the exploitation of new therapeutic tools / F. Zito ... [et al.] -- Cell signalling during sea urchin development : a model for assessing toxicity of environmnetal contaminants / C. Angelini ... [et al.] -- Echinoderm reactive oxygen species (ROS) production measured by peroxidase, luminol-enhanced chemiluminescence (PLCL) as an immunotoxicological tool / G. Coteur, B. Danis, P. Dubois -- Monitoring chemical and physical stress using sea urchin immune cells / V. Matranga ... [et al.] -- DNA damage and developmental defects after exposure to UV and heavy metals in sea urchin cells and embryos compared to other invertebrates / H.C. Schröder ... [et al.] -- Echinoderms : their culture and bioactive compounds / M.S. Kelly -- Regenerative response and endocrine disrupters in crinoid echinoderms : an old experimental model, a new ecotoxicological test / M.D. Candia Carnevali -- Echinoderm adhesive secretions : from experimental characterization to biotechnological applications / P. Flammang, R. Santos, D. Haesaerts -- Mutable collagenous tissue : overview and biotechnological perspective / I.C. Wilkie -- Bioresources from echinoderms / Y. Yokota.
  • 2014 Springer
    Christian R. Landry, Nadia Aubin-Horth, editors.
    "Researchers in the field of ecological genomics aim to determine how a genome or a population of genomes interacts with its environment across ecological and evolutionary timescales. Ecological genomics is trans-disciplinary by nature. Ecologists have turned to genomics to be able to elucidate the mechanistic bases of the biodiversity their research tries to understand. Genomicists have turned to ecology in order to better explain the functional cellular and molecular variation they observed in their model organisms. Ecological genomics covers 3 fields of research that have most benefited from the recent technological and conceptual developments in the field of ecological genomics: the study of life-history evolution and its impact of genome architectures; the study of the genomic bases of phenotypic plasticity and the study of the genomic bases of adaptation and speciation"-- Publisher's description.
    Also available: Print – 2014
  • 2002 Wiley
    C.U.M. Smith.
    ch. 1. Introductory orientation -- ch. 2. The Conformation of informational macromolecules -- ch. 3. Information processing in cells -- ch. 4. Molecular evolution -- ch. 5. Manipulating biomolecules -- ch. 6. Genomics -- ch. 7. Biomembranes -- ch. 8. G-protein-coupled receptors -- ch. 9. Pumps -- ch. 10. Ligand-gated ion channels -- ch. 11. Voltage-gated channels -- ch. 12. Resting potentials and cable conduction -- ch. 13. Sensory transduction -- ch. 14. The Action potential -- ch. 15. The Neuron as a secretory cell -- ch. 16. Neurotransmitters and neuromodulators -- ch. 17. The Postsynaptic cell -- ch. 18. Developmental genetics of the brain -- ch. 19. Epigenetics of the brain -- ch. 20. Memory -- ch. 21. Some pathologies.
  • 2013 Springer
    Michael K. Danquah, Ram I. Mahato, editors.
  • 2009 Springer
    Florian Lang (ed.).
  • 2006 Springer
    Detlev Ganten, Klaus Ruckpaul (eds.).
    v. 1. A-L -- v. 2. M-Z.
  • 2005 Springer Protocols
    edited by Tarun B. Patel, Paul J. Bertics.
    A historical perspective of EGF receptor and related systems / Francis Edwin ... [et al.] -- Purification and assay of kinase-active EGF receptor from mammalian cells by immunoaffinity chromatography / Gregory J. Wiepz ... [et al.] -- Soluble epidermal growth factor receptor acrydinium-linked immunosorbent assay / Jacqueline M. Lafky, Andre T. Baron, and Nita J. Maihle -- Analysis of epidermal growth factor receptor and interactions with the [alpha] subunit of the stimulatory GTP binding protein of adenylyl cyclase, Gs / Deepti Chaturvedi ... [et al.] -- Regulator of epidermal growth factor signaling : sprouty / Esther Sook Miin Wong and Graeme R. Guy -- Dissecting the epidermal growth factor signal transactivation pathway / Oliver M. Fischer, Stefan Hart, and Axel Ullrich -- A sensitive method to monitor ectodomain shedding of ligands of the epidermal growth factor receptor / Umut Sahin ... [et al.] -- In vitro and in vivo assays of monoubiquitnation of receptor tyrosine kinases / Manachem Katz, Yaron Mosesson, and Yosef Yarden -- Assays to monitor degradation of the EGF receptor / Mirko H.H. Schmidt and Ivan Dikic -- Assessment of degradation and ubiquitination of CXCR4, a GPCR regulated by EGFR family members / Adriano Marchese -- Epithelial cell migration in response to epidermal growth factor / Reema Zeineldin and Laurie G. Hudson -- Motility signaled from EGF receptors and related systems / Alan Wells ... [et al.] -- Methods for determining the proliferation of cells in response to EGFR ligands / Gregory J. Wiepz ... [et al.] -- Clinical advancement of EGFR inhibitors in cancer therapy / Prakash Chinnaiyan and Paul M. Harari.
  • 2011 Springer Protocols
    edited by Trygve O. Tollefsbol.
    1. Advances in epigenetic technology / Trygve O. Tollefsbol -- 2. DNA methylation detection: bisulfite genomic sequencing analysis / Yuanyuan Li and Trygve O. Tollefsbol -- 3. Methylation-specific PCR / Ja-Lok Ku, You-Kyung Jeon, and Jae-Gabb Park -- 4. Analysing DNA methylation using bisulphite pyrosequencing / Thomas Mikeska, Jörg Felsberg, Chelsee A. Hewitt, and Alexander Dobrovic -- 5. Closed-tube PCR methods for locus-specific DNA methylation analysis / Ida L.M. Candiloro, Thomas Mikeska, and Alexander Dobrovic -- 6. A combined bisulfite restriction analysis bioinformatics tool: methyl-typing / Cheng-Hong Yang, Yu-Huei Cheng, Li-Yeh Chuang, and Hsueh-Wei Chang -- 7. SIRPH: an HPLC-based SNuPE for quantitative methylation measurement at specific CpG sites / Heike Singer, Nicole Nüsgen, and Osman El-Maarri -- 8. Restriction landmark genome scanning / Hisato Okuizumi, Tomoko Takamiya, Yasushi Okazaki, and Yoshihide Hayashizaki -- 9. Methylated DNA immunoprecipitation genome-wide analysis / Mattia Pelizzola and Annette Molinaro -- 10. Methylated-CpG island recovery assay / Natalie Mitchell, J. Tyson DeAngelis, and Trygve O. Tollefsbol -- 11. Global DNA methylation analysis using the luminometric methylation assay / Mohsen Karimi, Karin Luttropp, and Tomas J. Ekström -- 12. Inhibition of DNA methylation in somatic cells / Angelica M. Giraldo and Kenneth R. Bondioli -- 13. DNA methyltransferase assays / Renata Z. Jurkowska, Alexandre Ceccaldi, Yingying Zhang, Paola B. Arimondo, and Albert Jeltsch -- 14. A chromatin immunoprecipitation protocol for small cell numbers / Philippe Collas -- 15. Native chromatin immunoprecipitation / Céline Cosseau and Christoph Grunau -- 16. Q-PCR in combination with ChIP assays to detect changes in chromatin acetylation / Ryan A. Irvine, Cindy Okitsu, and Chih-Lin Hsieh -- 17. Sequential chromatin immunoprecipitation assay and analysis / Ricardo B. de Medeiros -- 18. Combined chromatin immunoprecipitation and bisulfite methylation sequencing analysis / Yuanyuan Li and Trygve O. Tollefsbol -- 19. Studying RNA-protein interactions in vivo by RNA immunoprecipitation / Luke A. Selth, Pierre Close, and Jesper Q. Svejstrup -- 20. Using ChIP-seq technology to generate high-resolution profiles of histone modifications / Henriette O'Geen, Lorigail Echipare, and Peggy J. Farnham -- 21. Mapping open chromatin with formaldehyde-assisted isolation of regulatory elements / Takao Nammo, Santiago A. Rodriguez-Segui, and Jorge Ferrer -- 22. Inhibition of histone deacetylases / Yi Huang, Patrick G. Shaw, and Nancy E. Davidson -- 23. Computational methods for epigenetic analysis: the protocol of computational analysis for modified methylation-specific digital karyotyping based on massively parallel sequencing / Jian Li, Qian Zhao, and Lars Bolund.
  • 2015 Springer Protocols
    edited by Jason H. Moore, Scott M. Williams.
    Long-term selection experiments : epistasis and the response to selection / Charles Goodnight -- Finding the epistasis needles in the genome-wide haystack / Marylyn D. Ritchie -- Biological knowledge-driven analysis of epistasis in human GWAS with application to lipid traits / Li Ma, Alon Keinan, and Andrew G. Clark -- Epistasis for quantitative traits in Drosophila / Trudy F. C. Mackay -- Epistasis in the risk of human neuropsychiatric disease / Scott M. Williams -- On the partitioning of genetic variance with epistasis / José M. Álvarez-Castro and Arnaud Le Rouzic -- Measuring gene interactions / Thomas F. Hansen -- Two rules for the detection and quantification of epistasis and other interaction effects / Günter P. Wagner -- Direct approach to modeling epistasis / Rong-Cai Yang -- Capacitating epistasis -- detection and role in the genetic architecture of complex traits / Mats E. Pettersson and Örjan Carlborg -- Compositional epistasis : an epidemiologic perspective / Etsuji Suzuki and Tyler J. VanderWeele -- Identification of genome-wide SNP-SNP and SNP-clinical boolean interactions in age-related macular degeneration / Carlos Riveros [and eight others] -- Epistasis analysis using information theory / Jason H. Moore and Ting Hu -- Genome-wide epistasis and pleiotropy characterized by the bipartite human phenotype network / Christian Darabos and Jason H. Moore -- Network theory for data-driven epistasis networks / Caleb A. Lareau and Brett A. McKinney -- Epistasis analysis using multifactor dimensionality reduction / Jason H. Moore and Peter C. Andrews -- Epistasis analysis using ReliefF / Jason H. Moore -- Epistasis analysis using artificial intelligence / Jason H. Moore and Doug P. Hill.
  • 2010 Springer
    George A. Gerencser, editor.
    1. Chloride ATPase Pumps in Epithelia -- 2. Divalent Anion Transport in Crustacean and Molluscan Gastrointestinal Epithelia -- 3. Heavy Metal Transport and Detoxification by Crustacean Epithelial Lysosomes -- 4. Epithelial Calcium Transport in Crustaceans: Adaptation to Intrinsic and Extrinsic Stressors -- 5. The Cellular Basis of Extreme Alkali Secretion in Insects: A Tale of Two Tissues -- 6. H+, Na+, K+, and Amino Acid Transport in Caterpillar and Larval Mosquito Alimentary Canal -- 7. CFTR-Dependent Anion Transport in Airway Epithelia -- 8. Sulfate and Phosphate Transporters in Mammalian Renal and Gastrointestinal Systems -- 9. Role of H+-K+ ATPase, Na+-K+-2Cl⁻ and Na+-Cl⁻-HCO₃ ⁻ Transporters in Vertebrate Small Intestine -- 10. The H+- and H+, K+-ATPases of the Collecting Duct -- 11. Acid/Base Regulation in Renal Epithelia by H,K-ATPases -- 12. Sodium Transport Mechanisms in the Mammalian Nephron -- 13. Renal Acid-Base Regulation Via Ammonia Transport in Mammals -- 14. Hexose Transport Across Mammalian Epithelia -- 15. Amino Acid Transport by Epithelial Membranes -- 16. Molecular Ontology of Amino Acid Transport.
  • 2005 ScienceDirect
    edited by T. Ryan Gregory.
    Genome size evolution in animals / T. Ryan Gregory -- Genome size evolution in plants / Michael D. Bennett and Ilia J. Leitch -- Transposable elements / Margaret G. Kidwell -- B chromosomes / Juan Pedro M. Camacho -- Small-scale gene duplications / John S. Taylor and Jeroen Raes -- Large-scale gene and ancient genome duplications / Yves Van de Peer and Axel Meyer -- Polyploidy in plants / Jennifer A. Tate, Douglas E. Soltis, and Pamela S. Soltis -- Polyploidy in animals / T. Ryan Gregory and Barbara K. Mable -- Comparative genomics in eukaryotes / Alan Filipski and Sudhir Kumar -- Comparative genomics in prokaryotes / T. Ryan Gregory and Rob DeSalle -- Macroevolution and the genome / T. Ryan Gregory.
  • 2012 Springer Protocols
    Fulltext v. 2 (2012) Springer Protocols
    edited by Maria Anisimova.
    Vol. 1. -- pt. I. Introduction: Bioinformactician's primers: 1. Introduction to genome biology: features, processes, and structures / Aidan Budd ; 2. Diversity of genome organisation / Aidan Budd ; 3. Probability, statistics, and computational science / Niko Beerenwinkel and Juliane Siebourg ; 4. The essentials of computational molecular evolution / Stéphane Aris-Brosou and Nicolas Rodrigue ; -- pt. II. Genomic data assembly, alignment, and homology inference: 5. Next-generation sequencing technologies and fragment assembly algorithms / Heewook Lee and Haixu Tang ; 6. Gene prediction / Tyler Alioto ; 7. Alignment methods: strategies, challenges, benchmarking, and comparative overview / Ari Lötynoja ; 8. Whole-genome alignment / Colin N. Dewey ; 9. Inferring orthology and paralogy / Adrian M. Altenhoff and Christophe Dessimoz ; 10. Detecting laterally transferred genes / Rajeev K. Azad and Jeffrey G. Lawrence ; -- pt. III. Genome evolution: insights from statistical analyses: 11. Genome evolution in outcrossing versus selfing versus asexual species / Sylvain Glémin and Nicolas Galtier ; 12. Transposable elements and their identification / Wojciech Makałowski, Amit Pande, Valer Gotea, and Izabela Makałowska ; 13. Evolution of genome content: population dynamics of transposable elements in flies and humans / Josefa González and Dmitri Petrov ; 14. Detection and phylogenetic assessment of conserved synteny derived from whole genome duplications / Shigehiro Kuraku and Axel Meyer ; 15. Analysis of gene order evolution beyond single-copy genes / Nadia El-Mabrouk and David Sankoff ; 16. Discovering patterns in gene order / Laxmi Parida and Niina Haiminen -- Vol. 2. -- pt. I. Phylogenomics: 1. Tangled trees: the challenge of inferring species trees from coalescent and noncoalescent genes / Christian N.K. Anderson, Liang Liu, Dennis Pearl, and Scott V. Edwards ; 2. Modeling gene family evolution and reconciling phylogenetic discord / Gergely J. Szöllősi and Vincent Daubin ; 3. Genome-wide comparative analysis comparative analysis of phylogenetic trees: the Prokaryotic forest of life / Pere Puigbó, Yuri I. Wolf, and Eugene V. Koonin ; 4. Philosophy and evolution: minding the gap between evolutionary patterns and tree-like patterns / Eric Bapteste, Frédéric Bouchard, and Richard M. Burian ; -- pt. II. Natural selection, recombination, and innovation in genomic sequences: 5. Selection on the protein-coding genome / Carolin Kosiol and Maria Anisimova ; 6. Methods to detect selection on noncoding DNA / Ying Zhen and Peter Andolfatto ; 7. The origin and evolution of new genes / Margarida Cardoso-Moreiera and Manyuan Long ; 8. Evolution of protein domain architectures / Kristoffer Forslund and Erik L.L. Sonnhammer ; 9. Estimating recombination rates from genetic variation in humans / Adam Auton and Gil McVean ; 10. Evolution of viral genomes: interplay between selection, recombination and other forces / Sergei L. Kosakovsky Pond, Ben Murrell, and Art F.Y. Poon ; -- pt. III. Population genomics: 11. Association mapping and disease: evolutionary perspectives / Soren Besenbacher, Thomas Mailund, and Mikkel H. Schierup ; 12. Ancestral population genomics / Julien Y. Dutheil and Asger Hobolth ; 13. Nonredundant representation of ancestral recombinations graphs / Laxmi Parida ; -- pt. IV: The "-OMICS". 14. Using Genomic tools to study regulatory evolution / Yoav Gilad ; 15. Characterization and evolutionary analysis of protein-protein interaction networks / Gabriel Musso, Andrew Emili, and Zhaolei Zhang ; 16. Statistical methods in metabolomics / Alexander Korman, Amy Oh, Alexander Raskind, and David Banks ; 17. Introduction the analysis of environmental sequences: metagenomics with MEGAN / Daniel H. Huson and Suparna Mitra ; 18. Analyzing epigenome data in context of genome evolution and human diseases / Lars Feuerbach, Konstantin Halachev, Yassen Assenov, Fabian Müller, Cahristoph Bock, and Thomas Lengauer ; 19. Genetical genomics for evolutionary studies / Pjotr Prins, Geert Smant, and Ritsert C. Jansen ; -- pt. V. Handling genomic data: resources and computation: 20. Genomics data resources: frameworks and standards / Mark D. Wilkinson ; 21. Sharing programming resources between bio* projects through remote procedure call and native call stack strategies / Pjotr Prins, Naohisa Goto, Andrew Yates, Laurent Gautier, Scotter Willis, Christopher Fields, and Toshiaki Katayama ; 22. Scaleable computing for evoluntionart genomics / Pjotr Prins, Dominique Belhachemi, Steffen Möller, and Geert Smant.
  • [v.1], 2014
    Jonathan Orsay.
    Status: Not Checked OutLane Catalog Record
  • 2008 Springer Protocols
    [edited by] Andrei I. Ivanov.
    Vesicular trafficking : molecular tools and targets / Elena V. Vassilieva and Asma Nusrat -- Pharmacological inhibition of endocytic pathways : is it specific enough to be useful? / Andrei I. Ivanov -- In vitro assays to measure SNARE-mediated vesicle fusion / Susanne Kreye, Jörg Malsam, and Thomas H. Söllner -- Imaging Ca²⁺-triggered exocytosis of single secretory granules on plasma membrane lawns from neuroendocrine cells / Thorsten Lang -- FRAP analysis of secretory granule lipids and proteins in the sea urchin egg / Julian L. Wong and Gary M. Wessel -- A cell-free assay for endocytosis of E-cadherin / Toshiaki Sakisaka and Yoshimi Takai -- Cell-surface biotinylation to study endocytosis and recycling of occludin / Noriyuki Nishimura and Takuya Sasaki -- Fractionation of subcellular membrane vesicles of epithelial and nonepithelial cells by OptiPrep density gradient / Xuhang Li and Mark Donowitz -- Identification and characterization of interacting partners of Rab GTPases by yeast two-hybrid analyses / Mark Kail and Angelika Barnekow -- High-throughput analysis of the dynamics of recycling cell surface proteins / Roland Glovers, David E. James, and Adelle C.F. Coster -- Studying phagocytosis by live-cell scintillation proximity assay / Walter Stockinger and Axel Nohturfft -- Transcytosis of polymeric immunoglobin A in polarized Madin-Darby canine kidney cells / Asli Oztan, Christine Rondanino, and Gerard Apodaca -- The use of syntaxin chimeras to study polarized protein trafficking in epithelial cells / Martin B.A. ter Beest -- Targeting the epithelial SNARE machinery by bacterial neurotoxins / Véronique Proux-Gillardeaux and Thierry Galli -- Endocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor / Munekazu Yamakuchi ... [et al.] -- Molecular dissection of HCL secretion in gastric parietal cells using streptolysin O permeabilization / Xia Ding .. [et al.] -- Addressing membrane protein topology using the fluorescence protease protection (FPP) assay / Holger Lorenz, Dale W. Hailey, and Jennifer Lippincott-Schwartz -- Visualizing clathrin-mediated IgE receptor internalization by electron and atomic force microscopy / Alan R. Burns ... [et al.] -- Analyzing endosomes in nonsectioned cells by transmission electron microscopy / Willem Stoorvogel -- Imaging exocytosis of single insulin secretory granules with TIRF microscopy / Shinya Nagamatsu and Mica Ohara-Imaizumi -- Reconstitution of depolarization and Ca²⁺-evoked secretion in Xenopus oocytes monitored by membrane capacitance / Roy Cohen, Bernhard M. Schmitt, and Daphne Atlas -- Low-noise recording of single-vesicle capacitance steps in cell-attached patches / Vitaly Klyachko, Zhenjie Zhang, and Meyer Jackson -- Good practices in single-cell amperometry / David J. Machado, Mónica S. Montesinos, and Ricardo Borges -- Analysis of single-vesicle exocytotic events recorded by amperometry / Eugene V. Mosharov -- Application of RNAi technology and fluorescent protein markers to study membrane traffic in Caenorhabditis elegans / Dmitry Poteryaev and Anne Spang -- FM 1-43 labeling of synaptic vesicle pools at the Drosophila neuromuscular junction / Patrik Verstreken, Tomoko Ohyama, and Hugo J. Bellen -- Probing E-cadherin endocytosis by morpholino-mediated Rab5 knockdown in zebrafish / Florian Ulrich and Carl-Philipp Heisenberg -- Quantifying endocytosis in vivo using intravital two-photon microscopy / Ruben M. Sandoval and Bruce A. Molitoris.
  • 2012 Springer Protocols
    edited by Annemieke Aartsma-Rus.
    DNA diagnostics and exon skipping / Umasuthan Srirangalingam and Shern L. Chew -- Bioinformatics and mutations leading to exon skipping / F.O. Desmet and C. Béroud -- Minigenes to confirm exon skipping mutations / Lourdes R. Desviat, Belén Pérez, and Magdalena Ugarte -- Analysis and interpretation of RNA splicing alterations in genes involved in genetic disorders / Maaike P.G. Vreeswijk and Heleen M. van der Klift -- Exon skipping mutations in neurofibromatosis / Emanuele Buratti and Diana Baralle -- Overview on applications of antisense-mediated exon skipping / Willeke M.C. van Roon-Mom and Annemieke Aartsma-Rus -- Overview on DMD exon skipping / Annemieke Aartsma-Rus -- Overview on AON design / Annemieke Aartsma-Rus -- Optimizing RNA/ENA chimeric antisense oligonucleotides using in vitro splicing / Yasuhiro Takeshima, Mariko Yagi, and Masafumi Matsuo -- Optimizing antisense oligonucleotides using phophorodiamidate morpholino oligomers / Linda J. Popplewell, Alberto Malerba, and George Dickson -- Optimizing splice-switching oligomer sequences using 2'-O-methyl phosphorothioate chemistry / Carl Adkin, Sue Fletcher, and Steve D. Wilton -- Exon skipping quantification by real-time PCR / Alessandra Ferlini and Paola Rimessi -- Antisense-mediated exon skipping to shift alternative splicing to treat cancer / Jing Wan -- Antisense-mediated exon skipping to generate soluble receptors / A. Seda Yilmaz-Elis and J. Sjef Verbeek -- Antisense-mediated exon skipping to reframe transcripts / Sandrina Turczynski [and others] -- U1 snRNA as an effective vector for stable expression of antisense molecules and for the inhibition of the splicing reaction / Julie Martone, Fernanda Gabriella De Angelis, and Irene Bozzoni -- Engineering U7snRNA gene to reframe transcripts / Aurélie Goyenvalle -- Dynamic fluorescent and luminescent reporters for cell-based splicing screens / Claude C. Warzecha, Ruben Hovhannisyan, and Russ P. Carstens -- Antisense-mediated exon-skipping to induce gene knockdown / Petra Disterer and Bernard Khoo -- Antisense-mediated exon inclusion / Yimin Hua and Adrian R. Krainer -- Antisense genes to induce exon inclusion / Rachel Nlend Nlend and Daniel Schümperli -- Using mini-genes to identify factors that modulate alternative splicing / Robert Morse, Adrian G. Todd, and Philip J. Young -- Overview of alternative oligonucleotide chemistries for exon skipping / Amer F. Saleh, Andrey A. Arzumanov, and Michael J. Gait -- Identification of peptides for tissue-specific delivery / Hans Heemskerk -- Systemic delivery of antisense oligomer in animal models and its implications for treating DMD / Qi Long Lu and Bo Wu -- Cell-penetrating peptides enhance systemic delivery of antisense morpholino oligomers / Hong M. Moulton -- Optimizing tissue-specific antisense oligonucleotide-peptide conjugates / Corinne A. Betts [and others].
  • 2010 Springer
    Yo Kikuchi, Elena Y. Rykova (eds.).
    Extracellular DNA as Matrix Component in Microbial Biofilms / Wen-Chi Chiang and Tim Tolker-Nielsen -- Gene Transfer Agents and Defective Bacteriophages as Sources of Extracellular Prokaryotic DNA / Andrew S. Lang and J. Thomas Beatty -- Roles of Extracellular DNA in Bacterial Ecosystem / Katsuji Tani and Masao Nasu -- Stable Extracellular DNA: A Novel Substrate for Genetic Engineering that Mimics Horizontal Gene Transfer in Nature / Shinya Kaneko and Mitsuhiro Itaya -- Extracellular Nucleic Acids of the Marine Phototrophic Bacterium Rhodovulum sulfidophilum and Related Bacteria: Physiology and Biotechnology / Yo Kikuchi -- Systemic RNAi in C. elegans from the Viewpoint of RNA as Extracellular Signals / Lisa Timmons -- Circulating Nucleic Acids in Health and Disease / E. Y. Rykova, P. P. Laktionov and V. V. Vlassov -- Circulating MicroRNAs in Cancer / Patrick S. Mitchell and Muneesh Tewari -- Biology and Diagnostic Applications of Cell-Free Fetal Nucleic Acids in Maternal Plasma / Jason C. H. Tsang and Y. M. Dennis Lo -- The Biology of Circulating Nucleic Acids in Plasma and Serum (CNAPS) / P. B. Gahan and M. Stroun -- Modulation and Regulation of Gene Expression by CpG Oligonucleotides / Dennis Klinman and Sven Klaschik -- Immune Recognition of Nucleic Acids and Their Metabolites / Shohei Koyama, Shizuo Akira and Ken J. Ishii.
  • 2005 Springer
    [edited by] Mohammed S. Razzaque.
    Tissue scarring: lessons from wound healing / Mohammed S. Razzaque ... [et al.] -- Pathological significance of renal expression of proinflammatory molecules / Takashi Wada ... [et al.] -- Oxidative stress, lipoproteins, and angiotensin II-the unholy triade in the pathogenesis of renal fibrosis / Jan Galle, Thomas Quaschning and Stefan Seibold -- Involvement of NF-kB in renal inflammation and sclerosis / Laurent Baud -- Low-density lipoprotein and glomerulosclerosis / Hyun Soon Lee -- Molecular developments in the treatment of renal fibrosis / Gavin J. Becker and Tim D. Hewitson -- Myocardial infarction and cardiac fibrogenesis / Shozo Kusachi and Yoshifumi Ninomiya -- Cardiac fibrosis and aging / Serge Masson ... [et al.] -- Matrix remodeling and atherosclerosis effect of age / Ladislas Robert -- Molecular and cellular aspect of liver fibrosis / Norifumi Kawada -- Recent therapeutic developments in hepatic fibrosis / Ichiro Shimizu -- Reversibility of liver fibrosis: role of matrix metalloproteinases / Isao Okazaki ... [et al.] -- Can manipulation of apoptotic cell death benefit tissue scarring? / Wesam Ahmed, Mohammed S. Razzaque, and Takashi Taguchi -- Pulmonary fibrogenesis: the role of apoptosis and its clinical potentials / Kazuyoshi Kuwano, Naoki Hagimata, and Nobuyuki Hara -- Silica-induced inflammatory mediators and pulmonary fibrosis / Andrea K. Hubbard ... [et al.].
  • 2010 Springer Protocols
    edited by Joanna M. Bridger, Emanuela V. Volpi.
    Fluorescence in situ Hybridization (FISH), Basic principles and methodology -- Fluorescence in situ hybridization on DNA halo preparations and extended chromatin fibres -- Detection of nascent RNA transcripts by fluorescence in situ hybridization -- Fluorescence in situ hybridization analysis of formalin fixed paraffin embedded tissues, including tissue microarrays -- Fluorescence in situ Hybridization (FISH) on tissue cryosections -- Multiplex fluorescence in situ hybridization (M-FISH) -- Optical mapping of protein-DNA complexes on chromatin fibers -- 3D-FISH on cultured cells combined with immunostaining -- The Comet-FISH assay for the analysis of DNA damage and repair -- Direct in situ hybridization with oligonucleotide functionalized quantum dot probes -- LNA-FISH for detection of MicroRNAs in frozen sections -- Chromosome orientation fluorescence in situ hybridization or strand-specific FISH -- Combinatorial oligo FISH: directed labeling of specific genome domains in differentially fixed cell material and live cells -- Simultaneous visualization of FISH signals and bromo-deoxyuridine incorporation by formamide-free DNA denaturation -- CryoFISH: fluorescence in situ hybridization on ultrathin cryosections -- Characterization of chromosomal rearrangements using multicolor-banding (MCB/m-band) -- Visualizing nucleic acids in living cells by fluorescence in vivo hybridization -- Quality control in FISH as part of a laboratory's quality management system -- FlashFISH: "same day" prenatal diagnosis of common chromosomal aneuploidies -- FISH for pre-implantation genetic diagnosis -- PNA-FISH on human sperm -- POD-FISH: a new technique for parental origin determination based on copy number variation polymorphism -- Sequence-based high resolution chromosomal comparative genomic hybridization (CGH) -- ImmunoFISH on isolated nuclei from paraffin-embedded biopsy material -- Fluorescence in situ hybridization on 3D cultures of tumor cells -- Simultaneous ultrasensitive subpopulation staining/hybridization in situ (SUSHI) in HIV-1 disease monitoring -- Detection of prokaryotic cells with fluorescence in situ hybridization -- FISH as a tool to investigate chromosome behavior in budding yeast -- FISH on chromosomes derived from the snail model organism biomphalaria glabrata -- Fluorescence in situ hybridization with bacterial artificial chromosomes (BACs) to mitotic heterochromatin of drosophila -- Three-dimensional fluorescence in situ hybridization in mouse embryos using repetitive probe sequences -- Fluorescence in situ hybridization (FISH) for genomic investigations in rat -- Fluorescence in situ hybridization on early porcine embryos -- FISH on 3D preserved bovine and murine preimplantation embryos.
  • 2011 Springer
    Rowen J.Y. Chang, Salvador Ventura, editors.
    1. Oxidative folding: coupling conformational folding and disulfide formation -- 2. The case of oxidative folding of ribonuclease A: factors impacting fold maturation of ER-processed proteins -- 3. Cystine knot folding in cyclotides -- 4. In vitro folding of single/double chain insulins and related proteins -- 5. Unfolding and refolding of disulfide proteins using the method disulfide scrambling -- 6. Oxidative protein folding with small molecules -- 7. Protein disulfide isomerase and the catalysis of oxidative protein folding -- 8. Allosteric disulfide bonds -- 9. The problem of expression of multidisulfide bonded recombinant proteins in E. coli -- 10. NMR-spectroscopic investigation of disulfide dynamics in unfolded states of proteins -- 11. A half-century of oxidative folding and protein disulfphide formation -- Index.
  • 2011 Springer Protocols
    edited by Fahd Al-Mulla.
    Regulatory and ethical Issues on the utilization of FFPE tissues in research / Catherine M. With, David L. Evers, and Jeffrey T. Mason -- Tissue microarrays : construction and uses / Carol B. Fowler ... [et al.] -- Standardization in immunohistology / Anthony S.-Y. Leong and Trishe Y.-M. Leong -- Multiple immunofluorescence labeling of formalin-fixed paraffin-embedded tissue / David Robertson and Clare M. Isacke -- Microwaves for chromogenic In situ hybridization / Anthony S.-Y. Leong and Zenobia Haffajee -- Automated analysis of FISH-stained HER2/neu samples with metafer / Christian Schunck and Eiman Mohammad -- Laser capture microdissection of FFPE tissue sections bridging the gap between microscopy and molecular analysis / Renate Burgemeister -- Nucleic acids extraction from laser microdissected FFPE tissue sections / Renate Burgemeister -- Microarray-based CGH and copy number analysis of FFPE samples / Fahd Al-Mulla -- Microarray profiling of DNA extracted from FFPE tissues using SNP 6.0 affymetrix platform / Marianne Tuefferd ... [et al.] -- Whole genome amplification of DNA extracted from FFPE tissues / Mira Bosso and Fahd Al-Mulla -- Pyrosequencing of DNA extracted from formalin-fixed paraffin-embedded tissue / Brendan Doyle, Ciaran O'Riain, and Kim Appleton -- Analysis of DNA methylation in FFPE tissues using the methylight technology / Ashraf Dallol ... [et al.] -- RT-PCR gene expression profiling of RNA from paraffin-embedded tissues prepared using a range of different fixatives and conditions / Mei-Lan Liu ... [et al.] -- RT-PCR-based gene expression profiling for cancer biomarker discovery from fixed, paraffin-embedded tissues / Aaron Scott ... [et al.] -- MicroRNA isolation from formalin-fixed, paraffin-embedded tissues / Aihua Liu and Xiaowei Xu -- Gene expression profiling of RNA extracted from FFPE tissues : NuGEN technologies' whole-transcriptome amplification system / Leah Turner, Joe Don Heath, and Nurith Kurn -- Protein mass spectrometry applications on FFPE tissue sections / Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason -- Alternative fixative to formalin fixation for molecular applications : the RCL2®-CS100 approach / Amelie Denouel ... [et al.].
  • 2006 Springer
    Clemens von Sonntag.
  • 2012 Springer
    Volker A. Erdmann, Jan Barciszewski, editors.
    RNAissance / Juergen Brosius -- Nucleic Acids as Therapeutics / Saraswat Pushpendra, Pareek Arvind and Bhandari Anil -- Thio Effects as a Tool for Mechanistic Studies of the Cleavage of RNA Phosphodiester Bonds: The Chemical Basis / Mikko Ora, Tuomas Lönnberg and Harri Lönnberg -- Off-Target Effects and Safety Aspects of Phosphorothioate Oligonucleotides / Martina Stessl, Christian R. Noe and Johannes Winkler -- Oligonucleotide Conjugates: Rationale, Synthesis, and Applications / Yashveer Singh, Pierre Murat, Nicolas Spinelli and Eric Defrancq -- CRISPR: A Bacterial Immunity System Based on Small RNAs / Rolf Wagner and Ümit Pul -- Antisense Technology: From Unique Laboratory Tool to Novel Anticancer Treatments / Christine Di Cresce, Colin Way, Mateusz Rytelewski, Saman Maleki Vareki and Supritha Nilam, et al. -- Antisense-Mediated Reduction of Eukaryotic Noncoding RNAs / Xue-hai Liang, Timothy A. Vickers and Stanley T. Crooke -- Antisense Oligonucleotides in the Treatment of Malignant Gliomas / Gerardo Caruso, Mariella Caffo, Giuseppe Raudino, Federica Raudino and Mario Venza, et al. -- Natural Antisense Transcripts Mediate Regulation of Gene Expression / Marco Magistri and Mohammad Ali Faghihi -- Activation and Deactivation of Antisense and RNA Interference Function with Light / Jeane M. Govan and Alexander Deiters -- Ribozymes as Molecular Biology Reagents / Sanchita Bhadra, Arti Pothukuchy and Andrew Ellington -- RNA Technologies for Mitochondrial Genetics / André Dietrich, Katarzyna Rolle, Marta Gabryelska, Eliza Wyszko and Romain Val, et al. -- RNA-Based Therapies for Inherited Metabolic Diseases / Belén Pérez, Magdalena Ugarte and Lourdes R. Desviat -- RNA-Cleaving DNA Enzymes and Their Potential Therapeutic Applications as Antibacterial and Antiviral Agents / P. I. Pradeepkumar and Claudia Höbartner -- Hammerhead Ribozymes Against Virus and Viroid RNAs / Alberto Carbonell, Ricardo Flores and Selma Gago -- Suppression of Hepatitis C Viral Genome Replication with RNA-Cleaving Deoxyribozyme / Dal-Hee Min and Dong-Eun Kim -- Design of Synthetic shRNAs for Targeting Hepatitis C: A New Approach to Antiviral Therapeutics / Brian H. Johnston and Qing Ge -- The Diverse Active Sites in Splicing, Debranching, and MicroRNA Processing Around RNA Phosphodiester Bonds / Sourav Kumar Dey, Eduardo Paredes, Molly Evans and Subha R. Das -- MicroRNAs and Their Antagonists as Novel Therapeutics / Jeppe Vinther, Jakob Lewin Rukov and Noam Shomron -- microRNAs in Human Diseases and Viral Infections / Alessandra Mescalchin and Tobias Restle -- Dysregulation of MicroRNA Expression and Human Diseases? / To-Ha Thai -- Noncoding RNAs: Identification of Cancer-Associated MicroRNAs / Muller Fabbri and George A. Calin -- Targeting Non-coding RNAs for Cancer Therapy / Satya K. Kota and Savithri Balasubramanian -- MicroRNA Pathways in Drosophila / Geetanjali Chawla and Nicholas S. Sokol -- Viroids: The Smallest Known Infectious Agents Cause Accumulation of Viroid-Specific Small RNAs / Jaroslav Matoušek, Detlev Riesner and Gerhard Steger.
  • 2012 Springer Protocols
    edited by Michael Kaufmann and Claudia Klinger.
    Prediction of protein tertiary structures using MUFOLD -- Prediction of protein functions -- Genome-wide screens for expressed hypothetical proteins -- Self-custom-made SFP arrays for nonmodel organisms -- Construction and analysis of full-length and normalized cdna libraries from citrus -- Assembling linear DNA templates for in vitro transcription and translation -- Automated computational analysis of genome-wide DNA methylation profiling data from help-tagging assays -- Detection of RNA editing events in human cells using high-throughput sequencing -- Comparative study of differential gene expression in closely related bacterial species by comparative hybridization -- Whole-genome RT-qPCR microRNA expression profiling -- Using quantitative real-time reverse transcriptase polymerase chain reaction to validate gene regulation by pttg -- FRET-based real-time DNA microarrays -- 2-D gel electrophoresis: Constructing 2D-gel proteome reference maps -- The use of antigen microarrays in antibody profiling -- Limited proteolysis in proteomics using protease-immobilized microreactors -- Mass spectrometry for protein quantification in biomarker discovery -- High-throughput microtitre plate-based assay for DNA topoisomerases -- Microscale thermophoresis as a sensitive method to quantify protein: Nucleic acid interactions in solution -- Bioluminescence resonance energy transfer: An emerging tool for the detection of protein-protein interaction in living cells -- LuMPIS: Luciferase-based MBP-pull-down protein interaction screening system -- Yeast two-hybrid screens: Improvement of array-based screening results by N- and C-terminally tagged fusion proteins -- Inducible microRNA-mediated knockdown of the endogenous human lamin A/C gene -- Multiple-gene silencing using antisense RNAs in escherichia coli -- Functional screen of zebrafish deubiquitylating enzymes by morpholino knockdown and in situ hybridization -- Silencing of gene expression by gymnotic delivery of antisense oligonucleotides -- Polycistronic expression of interfering RNAs from RNA polymerase III promoters -- Metabolite analysis of cannabis sativa l. By NMR spectroscopy -- Metabolome analysis of gram-positive bacteria such as staphylococcus aureus by GC-MS and LC-MS -- Metabolic fingerprinting using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry.
  • 2008 Springer Protocols
    edited by Julie D. Thompson, Christine Schaeffer-Reiss, Marius Ueffing.
    A brief summary of the different types of mass spectrometers used in proteomics / Christine Schaeffer-Reiss -- Experimental setups and considerations to study microbial interactions / Petter Melin -- Plant proteomics / Eric Sarnighausen and Ralf Reski -- Methods for human CD8+ T lymphocyte proteome analysis / Lynne Thadikkaran ... [et al.] -- Label-free proteomics of serum / Natalia Govorukhina, Peter Horvatovich, and Rainer Bischoff -- Flow cytometric analysis of cell membrane microparticles / Monique P. Gelderman and Jan Simak -- Exosomes / Joost P.J.J. Hegmans, Peter J. Gerber, and Bart N. Lambrecht -- Toward a full characterization of the human 20S proteasome subunits and their isoforms by a combination of proteomic approaches / Sandrine Uttenweiler-Joseph ... [et al.] -- Free-flow electrophoresis of the human urinary proteome / Mikkel Nissum and Robert Wildgruber -- Versatile screening for binary protein-protein interactions by yeast two-hybrid mating / Stef J.F. Letteboer and Ronald Roepman -- Native fractionation : isolation of native membrane-bound protein complexes from porcine rod outer segments using isopycnic density gradient centrifugation / Magdalena Swiatek-de Lange, Bernd Müller, and Marius Ueffing -- Mapping the signaling pathways by functional interaction proteomics / Alex von Kriegsheim, Christian Preisinger, and Walter Kolch -- Selection of recombinant antibodies by eukaryotic ribosome display / Mingyue He and Michael J. Taussig -- Production of protein arrays by cell-free systems / Mingyue He and Michael J. Taussig -- Nondenaturing mass spectrometry to study noncovalent protein/protein and protein/ligand complexes : technical aspects and application to the determination of binding stoichiometries / Sarah Sanglier ... [et al.] -- Protein processing characterized by a gel-free proteomics approach / Petra Van Damme ... [et al.] -- Identification and characterization of N-glycosylated proteins using proteomics / David S. Selby ... [et al.] -- Data standards and controlled vocabularies for proteomics / Lennart Martens, Luisa Montecchi Palazzi, and Henning Hermjakob -- The PRIDE proteomics identifications database : data submission, query, and dataset comparison / Philip Jones and Richard Côté -- Searching the protein interaction space through the MINT database / Andrew Chatr-aryamontri ... [et al.] -- PepSeeker : mining information from proteomic data / Jennifer A. Siepen, Julian N. Selley, and Simon J. Hubbard -- Toward high-throughput and reliable peptide identification via MS/MS spectra / Jian Liu -- MassSorter : peptide mass fingerprinting data analysis / Ingvar Eidhammer, Harald Barsnes and Svein-Ole Mikalsen -- Database similarity searches / Frédéric Plewniak -- Protein multiple sequence alignment / Chuong B. Do and Kazutaka Katoh -- Discovering biomedical knowledge from the literature / Jasmin Šaríc, Henriette Engelken, and Uwe Reyle -- Protein subcellular localization prediction using artificial intelligence technology / Rajesh Nair and Burkhard Rost -- Protein functional annotation by homology / Raja Mazumder, Sona Vasudevan, and Anastasia N. Nikolskaya -- Designability and disease / Philip Wong and Dmitrij Frishman -- Prism : protein-protein interaction prediction by structural matching / Ozlem Keskin, Ruth Nussinov, and Attila Gursoy -- Prediction of protein interaction based on similarity of phylogenetic trees / Florencio Pazos ... [et al.] -- Large multiprotein structures modeling and simulation : the need for mesoscopic models / Antoine Coulon, Guillaume Beslon, and Olivier Gandrillon -- Dynamic pathway modeling of signal transduction networks : a domain-oriented approach / Holger Conzelmann and Ernst-Dieter Gilles.
  • 2014 Springer
    Jerry L. Workman, Susan M. Abmayr, editors.
    Chromatin is the combination of DNA and proteins that make up the genetic material of chromosomes. It is essential for packaging DNA, regulation of gene expression, DNA replication and repair. The audience for this book includes newly established scientists, graduate students and scientists seeking detailed overviews of various aspects of chromatin structure and function to gain entry into this field. The chapters in this book give a strong, updated groundwork about the fundamentals of chromatin which are essential to understand epigenetics. Some the fundamentals that this book covers include the structure and biochemistry of chromatin and the enzyme complexes that manage it.
  • 2013 Springer Protocols
    edited by Louise O'Connor and Barry Glynn.
    Overview of invasive fungal infections / Nina L. Tuite and Katrina Lacey -- Fungal diagnostics : review of commercially available methods / Javier Yugueros Marcos and David H. Pincus -- Rapid methods for the extraction and archiving of molecular grade fungal genomic DNA / Andrew M. Borman, Michael Palmer, and Elizabeth M. Johnson -- APEX DNA microarray for the identification of pathogenic fungi / Arianna Tavanti, Stefano Landi, and Sonia Senesi -- Microscopic detection of yeasts using fluorescence in situ hybridization / João Inácio and Maria da Luz Martins -- Quantitative detection of Aspergillus spp. by real-time nucleic acid sequence-based amplification / Yanan Zhao and David S. Perlin -- Differentiation of fungi using hybridization probes on the LightCycler® / Stephan Fricke [and others] -- Quantitative and multiplex detection of pathogenic fungi using padlock probes, generic qPCR, and suspension array readout / Magnus Jobs, Ronnie Eriksson, and Jonas Blomberg -- Species-specific identification of a wide range of clinically relevant fungal pathogens by the Luminex® xMAP technology / S. Preuner and T. Lion -- Applied gene histopathology : identification of Fusarium species in FFPE tissue sections by in situ hybridization / Yoichiro Okubo [and others] -- Application of chip-based flow cytometry for amphotericin B and fluconazole susceptibility testing on Candida strains / Orsolya Bouquet [and others] -- Surface plasmon resonance genosensor for the detection of Fusarium culmorum / Michelangelo Pascale, Francesco Zezza, and Giancarlo Perrone -- Hyperbranching rolling circle amplification, an improved protocol for discriminating between closely related fungal species / Jiufeng Sun and Sybren de Hoog -- Loop-mediated isothermal amplification-based detection of Fusarium graminearum / Ludwig Niessen -- Multiplex-tandem PCR for fungal diagnostics / Anna Lau, Keith Stanley, and Tania Sorrell -- Specific detection of Pneumocystis jirovecii in clinical samples by flow cytometry / Joana Barbosa [and others].
  • 2012 Springer
    edited by Monika Fuxreiter, Peter Tompa.
    Fuzzy complexes : a more stochastic view of protein function / Monika Fuxreiter and Peter Tompa -- Dynamic fuzziness during linker histone action / Steven J. McBryant and Jeffrey C. Hansen -- Intrinsic protein flexibility in regulation of cell proliferation : advantages for signaling and opportunities for novel therapeutics / Ariele Viacava Follis, Charles A. Galea, and Richard W. Kriwacki -- Interplay between protein order, disorder and oligomericity in receptor signaling / Alexander B. Sigalov -- Consequences of fuzziness in the NFB/IB interaction / Elizabeth A. Komives -- Roles for intrinsic disorder and ̂fuzziness in generating context specific function in ultrabithorax : a hox transcription factor / Sarah E. Bondos and Hao Ching Hsiao -- Molecular recognition by the EWS transcriptional activation domain / Kevin A.W. Lee -- The measles virus NTAILXD complex : an illustrative example of fuzziness / Sonia Longhi -- Fuzziness in the core of the human pathogenic viruses HCV and HIV / Roland Ivanyi Nagy and Jean Luc Darlix -- Structural disorder and protein elasticity / Sarah Rauscher and Røgis Pomès -- "Fuzziness" in the cellular interactome: a historical perspective / G. Rickey Welch.
    Also available: Print – 2012
  • 2006 CRCnetBASE
    edited by Tatsuya Haga, Shigeki Takeda.
  • 2011 CRCnetBASE
    William Wu, Helen H. Zhang, Michael J. Welsh, and Peter B. Kaufman.
    "The third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. Other topics include approaches to grant funding and SiRNA technology. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. This edition features new techniques for every chapter, as well as several new chapters"--Provided by publisher.
  • 2015 Springer Protocols
    edited by Xuenong Bo, Joost Verhaagen.
    Adeno-Associated Viral Vectors for Gene Delivery to the Nervous System -- Lentiviral Vectors for Gene Delivery to the Nervous System -- Gene Therapy for Parkinson's Disease: AAV-5-Mediated Delivery of Glial Cell Line-Derived Neurotrophic Factor (GDNF) -- Gene Delivery and Gene Therapy for Alzheimer's Disease -- Gene Therapy for Huntington's Disease -- Gene Therapy Approaches to Promoting Axonal Regeneration After Spinal Cord Injury -- Gene Delivery to Neurons of the Dorsal Root Ganglia Using Adeno-Associated Viral Vectors -- Targeted Gene Therapy for Ischemic Stroke -- Adeno-Associated Viral Gene Therapy for Retinal Disorders -- Gene Therapy for Epilepsies -- AAV Gene Therapy Strategies for Lysosomal Storage Disorders with Central Nervous System Involvement -- Gene Therapy in Spinal Muscular Atrophy (SMA) Models Using Intracerebroventricular Injection into Neonatal Mice -- Gene Therapy for Chronic Pain: How to Manipulate and Unravel Pain Control Circuits from the Brain? -- Gene Therapy Approaches Using Reproducible and Fully Penetrant Lentivirus-Mediated Endogenous Glioma Models
  • 2015 Springer Protocols
    edited by Long Jason Lu.
    Microarray Transposon Tracking for the Mapping of Conditionally Essential Genes in Campylobacter jejuni -- Identifying Essential Streptococcus sanguinis Genes Using Genome-Wide Deletion Mutation -- Defining Essential Genes and Identifying Virulence Factors of Porphyromonas gingivalis by Massively-Parallel Sequencing of Transposon Libraries (Tn-seq) -- Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12 -- Identification of Genes Essential for Leptospirosis -- Identifying Essential Genes in Mycobacterium tuberculosis by Global Phenotypic Profiling -- Essential Genes in the Infection Model of Pseudomonas aeruginosa-PCR-Based Signature-Tagged Mutagenesis -- Genome-Wide Synthetic Genetic Screening by Transposon Mutagenesis in Candida albicans -- An Integrated Machine-Learning Model to Predict Prokaryotic Essential Genes -- A Statistical Framework for Improving Genomic Annotations of Transposon Mutagenesis (TM) Assigned Essential Genes -- A Proposed Essential Gene Discovery Pipeline: A Campylobacter jejuni Case Study -- Computational Prediction of Essential Metabolic Genes Using Constraint-Based Approaches -- Three Computational Tools for Predicting Bacterial Essential Genes -- Gene Essentiality Analysis Based on DEG 10, an Updated Database of Essential Genes -- Discovering Essential Domains in Essential Genes.
  • 2011 Springer Protocols
    edited by Lorraine O'Driscoll.
    Real-time PCR and multiplex approaches / Olga L. Gurvich and Mikhail Skoblov -- Reverse-transcriptase polymerase chain reaction to detect extracellular mRNAs / Sweta Rani and Lorraine O'Driscoll -- Microarray analysis of mRNAs : experimental design and data analysis fundamentals / Jai Prakash Mehta -- Software and tools for microarray data analysis / Jai Prakash Mehta and Sweta Rani -- Analysis of gene expression as relevant to cancer cells and circulating tumour cells / Anne M. Friel, John Crown, and Lorraine O'Driscoll -- Gene expression profiling in formalin-fixed, paraffin-embedded tissues using the whole-genome DASL assay / Craig S. April and Jian-Bing Fan -- MicroRNA expression analysis : techniques suitable for studies of intercellular and extracellular MicroRNAs / Erica Hennessy and Lorraine O'Driscoll -- Western blotting analysis as a tool to study receptor tyrosine kinases / Serena Germano and Lorraine O'Driscoll -- 2D gel electrophoresis and mass spectrometry identification and analysis of proteins / Paula Meleady -- Design, construction, and analysis of cell line arrays and tissue microarrays for gene expression analysis / Kathy Gately, Keith Kerr, and Ken O'Byrne -- Immunohistochemical and immunofluorescence procedures for protein analysis / Kishore Reddy Katikireddy and Finbarr O'Sullivan -- Advanced microscopy : laser scanning confocal microscopy / Orla Hanrahan, James Harris, and Chris Egan -- Isolation of exosomes for subsequent mRNA, microRNA, and protein profiling / Sweta Rani [and others] -- Atomic force microscopy and high-content analysis : two innovative technologies for dissecting the relationship between epithelial-mesenchymal transition-related morphological and structural alterations and cell mechanical properties / Stephen T. Buckley, Anthony M. Davies, and Carsten Ehrhardt.
  • 2012 Springer Protocols
    edited by Bart Deplancke, Nele Gheldof.
    pt. I. Regulatory state components. 1. How do you find transcription factors? Computational approaches to compile and annotate repertoires of regulators for any genome / Juan M. Vaquerizas, Sarah A. Teichmann, and Nicholas M. Luscombe. 2. Expression pattern analysis of regulatory transcription factors in Caenorhabditis elegans / Huiyun Feng, Hannah L. Craig, and Ian A. Hope. 3. High-throughput SELEX determination of DNA sequences bound by transcription factors in vitro / Nobuo Ogawa and Mark D. Biggin. 4. Convenient determination of protein-binding DNA sequences using quadruple 9-mer-based microarray and DsRed-monomer fusion protein / Min-Jeong Kim, Pil Joong Chung, Tae-Ho Lee, Tae-Hoon Kim, Baek Hie Nahm, and Yeon-Ki Kim. 5. Analysis of specific protein-DNA interactions by bacterial one-hybrid assay / Marcus B. Noyes. 6. MITOMI : a microfluidic platform for in vitro characterization of transcription factor-DNA interaction / Sylvie Rockel, Marcel Geertz, and Sebastian J. Maerkl. 7. Detecting protein-protein interactions with the split-ubiquitin sensor / Alexander Dünkler, Judith Müller, and Nils Johnsson. 8. Genome-wide dissection of posttranscriptional and posttranslational interactions / Mukesh Bansal and Andrea Califano. 9. Linking cellular signalling to gene expression using EXT-encoded reporter libraries / Anna Botvinik and Moritz J. Rossner. 10. Sample preparation for small RNA massive parallel sequencing / Willemijn M. Gommans and Eugene Berezikov -- pt. II. Genomic components. 11. CAGE (cap analysis of gene expression) : a protocol for the detection of promoter and transcriptional networks / Hazuki Takahashi, Sachi Kato, Mitsuyoshi Murata, and Piero Carninci. 12. Detecting DNaseI-hypersensitivity sites with MLPA / Thomas Ohnesorg, Stefanie Eggers, and Stefan J. White. 13. Detecting long-range chromatin interactions using the chromosome conformation capture sequencing (4C-seq) method / Nele Gheldof, Marion Leleu, Daan Noordermeer, Jacques Rougemont, and Alexandre Reymond -- pt. III. Mapping protein-DNA interactions. 14. Analyzing transcription factor occupancy during embryo development using ChIP-seq / Yad Ghavi-Helm and Eileen E.M. Furlong. 15. Genome-wide profiling of DNA-binding proteins using barcode-based multiplex solexa sequencing / Sunil Kumar Raghav and Bart Deplancke. 16. Computational analysis of protein-DNA interactions from ChIP-seq data / Jacques Rougemont and Felix Naef. 17. Using a yeast inverse one-hybrid system to identify functional binding sites of transcription factors / Jizhou Yan and Shawn M. Burgess. 18. Using cisTargetX to predict transcriptional targets and networks in Drosophila / Delphine Potier, Zeynep Kalender Atak, Marina Naval Sanchez, Carl Herrmann, and Stein Aerts. 19. Proteomic methodologies to study transcription factor function / Harry W. Jarrett. 20. High-throughput gateway-compatible yeast one-hybrid screen to detect protein-DNA interactions / Korneel Hens, Jean-Daniel Feuz, and Bart Deplancke -- pt. IV. Visualization GRNs. 21. BioTapestry : a tool to visualize the dynamic properties of gene regulatory networks / William J.R. Longabaugh -- pt. V. Modeling of GRNs. 22. Implicit methods for qualitative modeling of gene regulatory networks / Abhishek Garg, Kartik Mohanram, Giovanni De Micheli, and Ioannis Xenarios.
  • 2012 Springer Protocols
    edited by Jean Peccoud.
    Part I. Assembly of oligonucleotides in synthons. Building block synthesis using the polymerase chain assembly method / Julie A. Marchand and Jean Peccoud. Oligonucleotide assembly in yeast to produce synthetic DNA fragments / Daniel G. Gibson. Topdown real-time gene synthesis / Mo Chao Huang [and others] De novo DNA synthesis using single-molecule PCR / Tuval Ben Yehezekel, Gregory Linshiz, and Ehud Shapiro -- Part II. Synthon assembly. SLIC : a method for sequence- and ligation-independent cloning / Mamie Z. Li and Stephen J. Elledge. Assembly of standardized DNA parts using bioBrick ends in E. coli / Olivia Ho-Shing [and others] Assembling DNA fragments by USER fusion / Narayana Annaluru [and others] Fusion PCR via novel overlap sequences / Kamonchai Cha-aim [and others] Using recombineering to generate point mutations : the oligonucleotide-based "Hit and Fix" method / Suhwan Chang, Stacey Stauffer, and Shyam K. Sharan. Using recombineering to generate point mutations : galK-based positive-negative selection method / Kajal Biswas, Stacey Stauffer, and Shyam K. Sharan. Assembling large DNA segments in yeast / Héloïse Muller [and others] Recursive construction of perfect DNA molecules and libraries from imperfect oligonucleotides / Gregory Linshiz, Tuval Ben Yehezkel, and Ehud Shapiro. Cloning whole bacterial genomes in yeast / Gwynedd A. Benders. Production of infectious poliovirus from synthetic viral genomes / Jeronimo Cello and Steffen Muller -- Part III. Software for gene synthesis. In silico design of functional DNA constructs / Alan Villalobos, Mark Welch, and Jeremy Minshull. Using DNAworks in designing oligonucleotides for PCR-based gene synthesis / David Hoover. De novo gene synthesis design using TmPrime software / Mo-Huang Li [and others] Design-a-gene with GeneDesign / Sarah M. Richardson [and others] -- Part IV. Education and security. Leading a successful iGEM team / Wayne Materi. The Build-a-Genome course / Eric M. Cooper [and others] DNA synthesis security / Ali Nouri and Christopher F. Chyba.
  • 2015 Springer
    Ben Berkhout, Hildegund C.J. Ertl, Marc S. Weinberg, editors.
    Gene therapies for hepatitis C virus / Monique M.A. Verstegen, Qiuwei Pan, and Luc J.W. van der Laan -- Recent advances in use of gene therapy to treat hepatitis B virus infection / Kristie Bloom, Abdullah Ely, and Patrick Arbuthnot -- U1interference (U1i) for antiviral approaches / Lorea Blázquez and Puri Fortes -- Gene therapy strategies to block HIV-1 replication by RNA interference / Elena Herrera-Carrillo and Ben Berkhout -- HIV and ribozymes / Robert J. Scarborough and Anne Gatignol -- Editing CCR5: a novel approach to HIV gene therapy / Tatjana I. Cornu, Claudio Mussolino, Kristie Bloom, and Toni Cathomen -- Synthetic DNA approach to cytomegalovirus vaccine/immune therapy / Stephan J. Wu, Daniel O. Villarreal, Devon J. Shedlock, and David B. Weiner -- Vector-mediated antibody gene transfer for infectious diseases / Bruce C. Schnepp and Philip R. Johnson -- HIV latency and the non-coding RNA therapeutic landscape / Sheena Saayman, Thomas C. Roberts, Kevin V. Morris, and Marc S. Weinberg -- C peptides as entry inhibitors for gene therapy / Lisa Egerer, Hans-Peter Kiem, and Dorothee von Laer -- Aptamer-siRNA chimeras for HIV / Mayumi Takahashi, John C. Burnett, and John J. Rossi -- Index.
    Also available: Print – 2015
  • v. 1-, 2008- Springer Protocols
    edited by Joseph Ledoux.
    v. 1. Production and in vivo applications of gene transfer vectors. Preparation and quantification of pseudotyped retroviral vector / Hong Yu and Young Jik Kwon. Production of retroviral vectors for clinical use / Kenneth Cornetta, Lilith Reeves, and Scott Cross. Methods for the production of helper-dependent adenoviral vectors / Donna J. Palmer and Philip Ng. Methods for the production of first generation adenoviral vectors / Donna J. Palmer and Philip Ng. Large-scale production of recombinant adeno-associated viral vectors / Alejandro Negrete and Robert M. Kotin. Construction and production of recombinant herpes simplex virus vectors / William F. Goins ... [et al.] Plasmid-based gene transfer in mouse skeletal muscle by electroporation / Jonathan D. Schertzer and Goron S. Lynch. Chitosan nanoparticle-mediated gene transfer / Dongwon Lee and Shyam S. Mohapatra. PEG-PEI copolymers for oligonucleotide delivery to cells and tissues / Gordon J. Lutz, Shashank R. Sirsi, and Jason H. Williams. Non-viral gene delivery with cationic liposome-DNA complexes / Kai K. Ewert ... [et al.] Applications of lentiviral vectors in noninvasive molecular imaging / Abhijit De ... [et al.] Retroviral modification of mesenchymal stem cells for gene therapy of hemophilia A / Christopher B. Doering. Transduction of murine hematopoietic stem cells and in vivo selection of gene-modified cells / Lucienne M. Ide, Elisabeth Javazon, and H. Trent Spencer. Assessment of CFTR function after gene transfer in vitro and in vivo / Uta Griesenbach ... [et al.] Oncolytic adenoviruses for cancer gene therapy / Ta-Chiang Liu, Stephen H. Thorne, and David H. Kirn. Design of trans-splicing adeno-associated viral vectors for Duchenne muscular dystrophy gene therapy / Yi Lai ... [et al.] Prevention of type 1 diabetes in NOD mice by genetic engineering of hematopoietic stem cells / Jessamyn Bagley, Charoui Tian, and John Iacomini. Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells / Mingjie Li and John J. Rossi. Nanoparticle-mediated gene delivery to the lung / Rajagopal Ramesh. Retroviral-mediated gene therapy for the differentiation of primary cells into a mineralizing osteoblastic phenotype / Jennifer E. Phillips and Andrés J. García. In vivo siRNA delivery to the mouse hypothalamus shows a role of the co-chaperone XAP2 in regulating TRH transcription / Stéphanie Decherf, Zahra Hassani, and Barbara A. Demeneix. Efficient retroviral gene transfer to epidermal stem cells / Pedro Lei and Stelios T. Andreadis.
  • 2012 ScienceDirect
    edited by Theodore Friedman.
    General principles of retrovirus vector design / Tammy Chang and Jiing-Kuan Yee -- Strategies for retrovirus-based correction of severe, combined immunodeficiency (SCID) / Alain Fischer, Salima Hacein-Bey-Abina, and Marina Cavazzana-Calvo -- Retrovirus and lentivirus vector design and methods of cell conditioning / Samantha Cooray, Steven J. Howe, and Adrian J. Thrasher -- Analysis of the clonal repertoire of gene-corrected cells in gene therapy / Anna Paruzynski ... [et al.] -- Developing novel lentiviral vectors into clinical products / Anna Leath and Kenneth Cornetta -- Lentivirus vectors in [beta]-thalassemia / Emmanuel Payen ... [et al.] -- Gene therapy for chronic granulomatous disease / Elizabeth M. Kang and Harry L. Malech -- Alternative splicing caused by lentiviral integration in the human genome / Arianna Moiani and Fulvio Mavilio -- Genotoxicity assay for gene therapy vectors in tumor prone mice / Eugenio Montini and Daniela Cesana -- Lentiviral hematopoietic cell gene therapy for X-linked adrenoleukodystrophy / Nathalie Cartier ... [et al.] -- Retroviral replicating vectors in cancer / Christopher R. Logg ... [et al.] -- Adeno-associated virus vectorology, manufacturing, and clinical applications / Joshua C. Grieger and R. Jude Samulski -- Gene delivery to the retina : from mouse to man / Jean Bennett, Daniel C. Chung, and Albert Maguire -- Generation of hairpin-based RNAi vectors for biological and therapeutic application / Ryan L. Boudreau and Beverly L. Davidson -- Recombinant adeno-associated viral vector reference standards / Philippe Moullier and Richard O. Snyder -- NIH oversight of human gene transfer research involving retroviral, lentiviral, and adeno-associated virus vectors and the role of the NIH Recombinant DNA Advisory Committee / Marina O'Reilly ... [et al.] -- Regulatory structures for gene therapy medicinal products in the European Union / Bettina Klug ... [et al.].
  • 2008 Springer
    by Avery A. Sandberg, John F. Stone.
    Also available: Print – 2008
  • 2003 Karger
    volume editors, B. Wissinger, Susanne Kohl, U. Langenbeck.
    A glimpse into genomeland / Langenbeck, U. -- Epidemiology of hereditary ocular disorders / Rosenberg, T. -- Interactions of genes and environment in myopia / Feldkämper, M., Schaeffel, F. -- A molecular perspective on corneal dystrophies / Vincent, A.L., Rootman, D., Munier, F.L., Héon, E. -- Molecular genetics of cataract / Hejtmancik, J.F., Smaoui, N. -- Progress in the genetics of glaucoma / Weisschuh, N., Schiefer U. -- LHON and other optic nerve atrophies: the mitochondrial connection / Howell, N. -- Retinitis pigmentosa: genes, proteins and prospects / Hims, M.M., Diager, S.P., Inglehearn, C.F. -- Bardet-Biedl syndrome and Usher syndrome / Koenig, R. -- Genetic defects in vitamin A metabolism of the retinal pigment epithelium / Thompson, D.A., Gal, A. -- Genetics of macular dystrophies and implications for age-related macular degeneration / Klaver, C.C.W., Allikmets, R. -- Genetics of color vision deficiencies / Deeb, S.S., Kohl, S. -- Gene therapy and animal models for retinal disease / Dejneka, N.S., Rex, T.S., Bennett, J. -- Support for patients loosing [sic] sight / Trauzettel-Klosinski, S., Hahn, G.0A. - Author index -- Subject index.
    Also available: Print – 2003
  • 2016 Springer
    Toni Cathomen, Matthew Hirsch, Matthew Porteus, editors.
    Gene editing 20 years later -- The development and use of zinc-finger nucleases -- The use and development of TAL effector nucleases -- Genome editing for neuromuscular diseases -- Phage integrases for genome editing -- Precise genome modification using triplex forming oligonucleotides and peptide nucleic acids -- Genome editing by Aptamer-Guided Gene Targeting (AGT) -- Stimulation of AAV gene editing via DSB repair -- Engineered nucleases and trinucleotide repeat diseases -- Using engineered nucleases to create HIV-resistant cells -- Strategies to determine off-target effects of engineered nucleases -- Cellular engineering and disease modeling with gene-editing nucleases -- Index.
    Also available: Print – 2016
  • 2012 Springer
    Paul Denny, Chittaranjan Kole, editors.
    Genomics in the sea urchin : new perspectives on a perennial model system / Katherine M. Buckley amd Jonathan P. Rast -- Genome mapping and genomics of Caenorhabditis elegans / Jonathan Hodgkin, Michael Paulini, and mary Ann Tuli -- Genome mapping and genomics in Drosophila / Boris Adryan and Steven Russell -- Genome structure, functional genomics, and proteomics in ascidians / Yasunori Sasakura, Nicolas Sierro, Kenta Nakai, Kazuo Inaba, and takehiro G. Kusakabe -- Punching above their weight : the compact genomes of pufferfishes / Brian Cusack and Hugues Roest Crollius -- Medaka genomics and the methods and resources for decoding genomic functions / Tetsuaki Kimura, Yasuhiro Kamei, Yusuke Takehana, Takao Sasado, and Kiyoshi Naruse -- Xenopus genomics and genetics : progress and prospects / Amy K. Sater and Michael J. Gilchrist -- Mouse genome mapping and genomics / Paul Denny -- Rat genome mapping and genetics / Claude Szpirer and Göran Levan.
  • 2002 NCBI Bookshelf
    T. A. Brown.
  • 2002 ebrary
    Michael Yudell and Robert DeSalle, editors.
    Making the genome public : the American Museum of Natural History and the coming age of genomics / Michael Yudell and Rob DeSalle -- Genome science and the new frontier -- Introduction / Nicholas Wade -- What does knowing about genomes mean for science and society? / Harold Varmus -- Sequencing the human genome : elucidating our genetic blueprint / Eric Green -- Whole-genome shotgun sequencing / J. Craig Venter -- After the genome: where should we go? / Leroy Hood -- Applications of genomics to medicine and agriculture -- Introduction / Robert Bazell -- Genomics : a rapid road from gene to patient / William Haseltine -- The origins of cancer and the human genome / Arnold J. Levine -- Gene diversity in the endorphin system: SNPs, chips, and possible implications / Mary Jeanne Kreek -- Genomics and biotechnology in agriculture / Barbara A. Schaal -- Exploring human variation : understanding identity in the genomic era -- Introduction / Rob DeSalle -- Using maternal and paternal genes to unlock human history / Douglas C. Wallace -- Eugenics, the genome, and human rights / Daniel J. Kevles -- Redesigning the self: the promise and perils of genetic enhancement / David J. Rothman and Sheila M. Rothman -- Financial, legal, and ethical issues and the new genomics -- Introduction / Michael Waldholz -- Investing in the biotechnology sector / Kris H. Jenner -- The role of patents in exploiting the genome / Rebecca Eisenberg -- Social side effects of the new human molecular genetic diagnostics / Troy Duster -- Mapping morality : the rights and wrongs of genomics / Arthur l. Caplan -- Summing up : finding our way through the revolution / Kathi E. Hanna.
  • 2012 Springer Protocols
    edited by Lars Feuk.
    What have studies of genomic disorders taught us about our genome / Alexandra D. Simmons, Claudia M.B. Carvalho, and James R. Lupski -- Microdeletion and microduplication syndromes / Lisenka E.L.M. Vissers and Paweł Stankiewicz -- Structural genomic variation in intellectual disability / Rolph Pfundt and Joris A. Veltman -- Copy number variation and psychiatric disease risk / Rebecca J. Levy [and others] -- Detection and characterization of copy number variation in autism spectrum disorder / Christian R. Marshall and Stephen W. Scherer -- Structural variation in subtelomeres / M. Katharine Rudd -- Array-based approaches in prenatal diagnosis / Paul D. Brady [and others] -- Structural variation and its effect on expression / Louise Harewood, Evelyne Chaignat, and Alexandre Reymond -- Challenges of studying complex and dynamic regions of the human genome / Edward J. Hollox -- Population genetic nature of copy number variation / Per Sjodin and Mattias Jakobsson -- Detection and interpretation of genomic structural variation in mammals / Ira M. Hall and Aaron R. Quinlan -- Structural genetic variation in the context of somatic mosaicism / Jan P. Dumanski and Arkadiusz Piotrowski -- Online resources for genomic structural variation / Tam P. Sneddon and Deanna M. Church -- Algorithm implementation for CNV discovery using affymetrix and illumina SNP array data / Laura Winchester and Jiannis Ragoussis -- Targeted screening and validation of copy number variations / Shana Ceulemans, Karlijn van der Ven, and Jurgen Del-Favero -- High-resolution copy number profiling by array CGH using DNA isolated from formalin-fixed, paraffin-embedded tissues / Hendrik F. van Essen and Bauke Ylstra -- Characterizing and interpreting genetic variation from personal genome sequencing / Anna C.V. Johansson and Lars Feuk -- Massively parallel sequencing approaches for characterization of structural variation / Daniel C. Koboldt [and others].
  • 2014 Springer
    György Marko-Varga, editor.
    This book offers a valuable resource that allows students, researchers, educators and the general public to learn about proteomics and genomics. Chromosomes form the basis for our genetic heritage and are the code for protein synthesis. The Human Genome Map was presented in 2002, and the Proteome Sequence Map is currently being created by a global consortia initiative. Proteome and genome building blocks already form the basis of scientific research areas and shape major areas of the pharmaceutical and biomedical industries. The book provides background information on and our current understanding of these gene and protein areas, and explains in detail how cutting-edge science is using these resources to develop new medicines and new diagnostics for patient treatment and care. The book will benefit all students and researchers who need a good understanding of genomics and proteomics within the clinical field. Its content is also suitable for a broad readership, including those not specialized in this field. Dr. Marko-Varga is head of Div. Clinical Protein Science & Imaging at the Biomedical Center, Dept. of Measurement Technology and Industrial Electrical Engineering, Lund University. He's also Professor at the 1st Department of Surgery, Tokyo Medical University, Tokyo, Japan.
  • 2008 Springer Protocols
    edited by Mike Starkey, Ramnath Elaswarapu.
    Protein profiling based on two-dimensional difference gel electrophoresis / Gert Van den Bergh and Lutgarde Arckens -- Quantitative protein profiling by mass spectrometry using isotope-coded affinity tags / Arsalan S. Haqqani, John F. Kelly, and Danica B. Stanimirovic -- Quantitative protein profiling by mass spectrometry using label-free proteomics / Arsala S. Haqqani, John F. Kelly, and Danica B. Stanimirovic -- Using 2D-LC-MS/MS to identify Francisella tularensis peptides in extracts from an infected mouse macrophage cell line / John F. Kelly and Wen Ding -- Baculovirus expression vector system : an emerging host for high-throughput eukaryotic protein expression / Binesh Shrestha, Carol Smee, and Opher Gileadi -- Coimmunoprecipitation and proteomic analyses / S. Fabio Falsone, Bernd Gesslbauer, and Andreas J. Kungl -- Tandem affinity purification combined with mass spectrometry to identify components of protein complexes / Peter Kaiser ... [et al.] -- Mammalian two-hybrid assay for detecting protein-protein interactioins in vivo / Runtao He and Xuguang Li -- Detection of protein-protein interactions in live cells and animals with split firefly luciferase protein fragment complementation / Victor Villalobos, Snehal Naik, and David Piwnica-Worms -- Subcellular localization of intracellular human proteins by construction of tagged fusion proteins and transient expression in COS-7 cells / John E. Collins -- GeneFAS : a tool for the prediction of gene function using multiple sources of data / Trupti Joshi ... [et al.] --Comparative genomics-based prediction of protein function / Toni Gabaldón -- Design, manufacture, and assay of the efficacy of siRNAs for gene silencing / Louise A. Dawson and Badar A. Usmani. Whole genome amplification with Phi29 DNA polymerase to enable genetic or genomic analysis of samples of low DNA yield / Kaisa Silander and Janna Saarela -- Scanning for DNA variants by denaturant capillary electrophoresis / Per O. Ekstrøm -- Identification of SNPs, or mutations in sequence chromatograms / Nicole Draper -- BeadArray-based genotyping / Helen Butler and Jiannis Ragoussis -- Microsattelite-based candidate gene linkage analysis studies / Cathryn Mellersh -- Full complexity genomic hybridization on 60-mer oligonucleotide microarrays for array comparative genomic hybridization (aCGH) / Alexei Protopopov, Bin Feng, and Lynda Chin -- Detection of copy number changes at multiple loci in DNA prepared from formalin-fixed, paraffin-embedded tissue by multiplex ligation -dependent probe amplification / Minoru Takata -- Application of microarrays for DNA methylation profiling / Axel Schumacher, Andreas Weinhäusl, and Arturas Petronis -- Genomewide identification of protein binding locations using chromatin immunoprecipitation coupled with microarray / Byung-Kwan Cho, Eric M. Knight, and Bernhard Ø. Palsson -- Transcriptional profiling of small samples in the central nervous system / Stephen D. Ginsberg -- Quantitative expression profiling of RNA from formalin-fixed, paraffin-embedded tissues using randomly assembled bead arrays / Marina Bibikova ... [et al.] -- Expression profiling of microRNAs in cancer cells : technical considerations / Mouldy Sioud and Lina Cekaite -- Identification of disease biomarkers by profiling of serum proteins using SELDI-TOF mass spectrometry / Sigrun Langbein -- The applicability of a cluster of differentiation monoclonal antibody microarray to the diagnosis of human disease / Peter Ellmark ... [et al.] --
  • 2013 Springer
    Tim Schedl, editor.
    Also available: Print – 2013
  • 2013 Springer
    Jolanta Barańska, editor.
    Also available: Print – 2013
  • David M. Glick.
  • 2006 Springer Protocols
    edited by Inka Brockhausen.
    Gene expression analysis of glycosylation-related genes by real-time polymerase chain reaction / Juan J. Garcia-Vallejo ... [et al.] -- Analysis of the glycodynamics of primary osteoblasts and bone cancer cells / Inka Brockhausen-Schutzbach, Xiaojing Yang, and Mark Harrison -- Micromethods for the characterization of lipid a core and o-antigen lipopolysaccharide / Cristina L. Marolda ... [et al.] -- Assays for a galactosyltransferase involved in the assembly of the O7 antigen repeat unit of escherichia coli / Inka Brockhausen-Schutzbach ... [et al.] -- High-throughput quantitation of metabolically labeled anionic glycoconjugates by scintillation proximity assay utilizing binding to cationic dyes / Karen J. Rees-Milton and Tassos P. Anastassiades -- Quantitative analysis of mucins in mucosal secretions using indirect ELISA / Pablo Argueso and Ilene K. Gipson -- Molecular dissection of the mouse zona pellucida: an electron microscopic perspective utilizing high-resolution colloidal-gold labeling methods / Frederick W. K. Kan -- Soluble adamantly glycosphingolipid analogs as probes of glycosphingolipid function / Clifford Lingwood ... [et al.] -- Diagnosis of Krabbe disease by use of a natural substrate / John W. Callahan and Marie-Anne Skomorowski -- In vitro assays of the functions of calnexin and calreticulin, lectin-chaperones of the endoplasmic reticulum / Breanna S. Ireland, Monika Niggemann, and David B. Williams -- Quantitative measurement of selectin-ligand interactions: assays to identify a sweet pill in a library of carbohydrates / Mark E. Beauharnois, Sriram Neelamegham, and Khushi L. Matta -- Sialic acid-binding specificities of siglecs / Nadine Bock and Soerge Kelm. The application of fluorophore-assisted carbohydrate -- Electrophoresis for the study of the dolichol pyrophosphate-linked oligosaccharides pathway in cell cultures and animal tissues / Ningguo Gao -- Partial purification of mannosylphosphorylundecaprenol synthase from micrococcus luteus: a useful enzyme for the biosynthesis of a variety of mannosylphosphorylpolyisoprenol products / Jeffrey S. Rush and Charles J. Waechter -- Assay of dolichyl-phospho-mannose synthase reconstituted in a lipid matrix / John S. Schutzbach -- O-mannosylation in mammalian cells / Tamao Endo and Hiroshi Manya -- Methods for analysis of unusual forms of o-glycosylation / Aleksandra Nita-Lazar and Robert S. Haltiwanger -- Structural analysis of naturally occurring sialic acids / Johannis P. Kamerling and Gerrit J. Gerwig -- Non-radioactive trans-sialidase screening assay / Silke Schrader and Roland Schauer -- Structural determination of o-glycans by tandem mass spectrometry / Catherine Robbe, Jean-Claude Michalski, and Calliope Capon -- Detailed structural analysis of n-glycans released from glycoproteins in SDS-page gel bands using HPLC combined with exoglycosidase array digestions / Louise Royle ... [et al.] -- Molecular modeling of glycosyltransferases / Anne Imberty ... [et al.] -- Galactoside_2,6-sialyltransferase (st6gal.i) and the sialyl_2,6-galactosyl-linkage in tissues and cell lines / Fabio Dall'Olio, Nadia Malagolini, and Mariella Chiricolo -- Visualizing intracellular distribution and activity of core 2 _(1,6)n-acetylglucosaminyltransferase-I (C2GNT-I) in living cells / Maelle Prorok-Hamon, Sylvie Mathieu, and Assou El-Battari --
  • 2013 Springer Protocols
    edited by Inka Brockhausen.
    Glycan microarray screening assay for glycosyltransferase specificities / W. Peng, C.M. Nycholat and N. Razi -- A fluorescence-based assay for core 1 [beta]3galactosyltransferase (T-synthase) activity / T. Ju and R.D. Cummings -- Structural and biochemical analysis of a bacterial glycosyltransferase / F. Zhu ... [et al.] -- Study of the biological functions of mucin type core 3 O-glycans / S.H. Lee and M. Fukuda -- Generation of anti-sulfated glycan antibodies using sulfotransferase-deficient mice / H. Kawashima -- Fluorescent microscopy as a tool to elucidate dysfunction and mislocalization of golgi glycosyltransferases in COG complex depleted mammalian cells / R.A. Willett, I.D. Pokrovskaya and V.V. Lupashin -- A practical approach to reconstruct evolutionary history of animal sialyltransferases and gain insights into the sequence-function relationships of golgi-glycosyltransferases / D. Petit ... [et al.] -- Fluorescent lectin staining of drosophila embryos and tissues to detect the spatial distribution of glycans during development / E. Tian, L. Zhang and K.G. Ten Hagen -- Photoaffinity labeling of protein O-mannosyltransferases of the PMT1/PMT2 subfamily / M. Loibl and S. Strahl -- Enzymatic analysis of the protein O-glycosyltransferase, rumi, acting toward epidermal growth factor-like (egf) repeats / H. Takeuchi and R.S. Haltiwanger -- Enzymatic characterization of recombinant enzymes of O-glcNAc cycling / E.J. Kim and J.A. Hanover -- Antibodies and activity measurements for the detection of o-glcnac transferase and assay of its substrate, UDP-glcNAc / T. Lefebvre ... [et al.] -- In vitro glycosylation assay for bacterial oligosaccharyltransferases / M.A. Musumeci, M.V. Ielmini and M.F. Feldman -- In vitro UDP-sugar:Undecaprenyl-phosphate sugar-1-phosphate transferase assay and product detection by thin layer chromatography / K.B. Patel and M.A. Valvano -- In vitro O-antigen ligase assay / X. Ruan and M.A. Valvano -- Functional identification of bacterial glucosyltransferase WbdN / Y. Gao, A. Vinnikova and I. Brockhausen -- Methods for the pasteurella glycosaminoglycan synthases: Enzymes that polymerize hyaluronan, chondroitin, or heparosan chains / P.L. Deangelis -- Methods for measuring class i membrane-bound hyaluronan synthase activity / P.H. Weigel, A.J. Padgett-McCue and B.A. Baggenstoss -- Creation and characterization of glycosyltransferase mutants of Trypanosoma brucei / L. Izquierdo, M.L. Guther and M.A. Ferguson -- Mannose-6-phosphate: A regulator of LLO destruction / N. Gao and M.A. Lehrman -- N-acetylglucosaminyltransferase (GnT) assays using fluorescent oligosaccharide acceptor substrates: GnT-III, IV, V, and IX (GnT-Vb) / S. Takamatsu ... [et al.] -- A method for determination of UDP-GlcNAc: GlcNAc[beta]1-6(GlcNAc[beta]1-2)man[alpha]1-r [GlcNAc to man] beta1-4n-acetylglucosaminyltransferase vi activity / T. Taguchi and N. Taniguchi -- In vitro assays of orphan glycosyltransferases and their application to identify notch xylosyltransferases / M.K. Sethi ... [et al.] -- In vitro folding of [beta]-1,4galactosyltransferase and polypeptide-[alpha]-N-acetylgalactosaminyltransferase from the inclusion bodies / B. Ramakrishnan and P.K. Qasba -- An assay for [alpha] 1,6-fucosyltransferase (FUT8) activity based on the HPLC separation of a reaction product with fluorescence detection / H. Ihara ... [et al.] -- Immunodetection of glycosyltransferases in gastrointestinal tissues / J. Gomes and C.A. Reis -- Glycosyltransferases in chemo-enzymatic synthesis of oligosaccharides / B. Tefsen and I. van Die -- Tumor targeting by a carbohydrate ligand-mimicking peptide / S. Hatakeyama ... [et al.] -- Glycoengineering of human cell lines using zinc finger nuclease gene targeting: Simple cells with homogeneous GalNAc O-glycosylation allow isolation of the O-glycoproteome by one-step lectin affinity chromatography / C. Steentoft, E.P. Bennett and H. Clausen -- Generation of monoclonal antibodies to native active human glycosyltransferases / M.B. Vester-Christensen ... [et al.].
  • 2007 Springer Protocols
    edited by Richard J. Sugrue.
    The use of monoclonal antibodies and lectins to identify changes in viral glycoproteins that are influenced by glycosylation : the case of respiratory syncytial virus attachment (G) glycoprotein / Joanna Rawling and José A. Melero -- Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS-CoV / Shuo Shen, Timothy H.P. Tan, and Yee-Joo Tan -- Analysis of glycoproteins of viruses in the family bunyaviridae / Xiaohong Shi and Richard M. Elliott -- Secretion of respiratory syncytial virus fusion protein from insect cells using the baculovirus expression system / Boon-Huan Tan, Gaie Brown, and Richard J. Sugrue -- Characterization of the dengue virus envelope glycoprotein expressed in Pichia pastoris / Boon-Huan Tan, Jian Lin Fu, and Richard J. Sugrue -- Cloning, expression, and functional analysis of patient-derived hepatitis C virus glycoproteins / Alexander W. Tarr ... [et al.]. Viruses and glycosylation : an overview / Richard J. Sugrue -- Interaction between respiratory syncytial virus and glycosaminoglycans, including heparan sulfate / Louay K. Hallak, Steven A. Kwilas, and Mark E. Peeples -- Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the baculovirus expression system / Sehaam Khan, Mah-Lee Ng, and Yee-Joo Tan -- The C type lectins DC-SIGN and L-SIGN : receptors for viral glycoproteins / Pierre-Yves Lozach ... [et al.] -- Functional analysis of the N-linked glycans within the fusion protein of respiratory syncytial virus / Ping Li ... [et al.] -- Expression and purification of viral glycoproteins using recombinant vaccinia viruses for functional and structural studies / Zhu-Nan Li and David A. Steinhauer -- The use of two-dimensional SDS-PAGE to analyze the glycan heterogeneity of the respiratory syncytial virus fusion protein / Terence P. McDonald and Richard J. Sugrue --
  • 2012 Springer
    Denis J. Dupré, Terence E. Hébert, Ralf Jockers, editors.
    ER-bound steps in the biosynthesis of G protein-coupled receptors / Christian Nanoff and Michael Freissmuth -- Role of chaperones in G Protein coupled receptor signaling complex assembly / Denis J. Dupré, Maha M. Hammad, Patrick Holland and Jaime Wertman -- GPCR oligomerization: contribution to receptor biogenesis / Kathleen Van Craenenbroeck -- The functional size of GPCRs: monomers, dimers or tetramers? / Darlaine Pétrin and Terence E. Hébert -- Regulation of post-Golgi traffic of G protein-coupled receptors / Guangyu Wu -- Regulated GPCR trafficking to the plasma membrane: general issues and the CCR5 chemokine receptor example / Hamasseh Shirvani, Gabriel Gätà and Stefano Marullo -- Regulatory processes governing the cell surface expression of LH and FSH receptors / Deborah L. Segaloff -- Chaperone-mediated assembly of G protein complexes / Barry M. Willardson and Christopher M. Tracy -- Synthesis and assembly of G protein [beta][gamma] dimers: comparison of in vitro and in vivo studies / Jane Dingus and John D. Hildebrandt -- Preferential assembly of G-[alpha][beta][gamma] complexes directed by the [gamma] subunits / Janet D. Robishaw -- G Protein trafficking / Philip B. Wedegaertner -- Differential assembly of GPCR signaling complexes determines signaling specificity / Pascal Maurice, Abla Benleulmi-Chaachoua and Ralf Jockers -- GPCR and voltage-gated calcium channels (VGCC) signaling complexes / Christophe Altier -- Pharmacological chaperones correct misfolded GPCRs and rescue function: protein trafficking as a therapeutic target / Guadalupe Maya-Núñez, Alfredo Ulloa-Aguirre, Jo Ann Janovick and P. Michael Conn.
  • 2002 Springer Protocols
    edited by Barry W. Hicks.
  • 2005 ScienceDirect
    edited by William E. Balch, Channing J. Der, Alan Hall.
    Also available: Print – 2005
  • 2005 ScienceDirect
    edited by William E. Balch, Channing J. Der, Alan Hall.
    Also available: Print – 2005
  • 2014 ScienceDirect
    edited by B. Anne Croy, Aureo T. Yamada, Francesco J. DeMayo, S. Lee Adamson.
    The Guide to Investigation of Mouse Pregnancy is the first publication to cover the mouse placenta or the angiogenic tree the mother develops to support the placenta. This much-needed resource covers monitoring of the cardiovascular system, gestational programming of chronic adult disease, epigenetic regulation, gene imprinting, and stem cells. Offering detailed and integrated information on how drugs, biologics, stress, and manipulations impact pregnancy in the mouse model, this reference highlights techniques used to analyze mouse pregnancy.
  • 2010 ScienceDirect
    volume editors, Jonathan Weissman, Christine Guthrie, Gerald R. Fink.
    Also available: Print – 2010
  • 2011 Springer
    Giorgio Stanta, editor.
  • 2010 CRCnetBASE
    edited by Roger L. Lundblad, Fiona M. Macdonald.
    "Offering a molecular perspective not available 20 years ago, [this edition] provides physical and chemical data on proteins, nucleic acids, lipids, and carbohydrates. Presented in an organized, concise, and simple-to-use format, this popular reference allows quick access to the most frequently used data. Covering a wide range of topics, from classical biochemistry to proteomics and genomics, it also details the properties of commonly used biochemicals, laboratory solvents, and reagents." --excerpts from book jacket.
  • 2006 CRCnetBASE
    SU Catalog (SearchWorks) Click LINK above for Print location/circulation status.
    edited by Srinivas Aluru.
  • v. 1-4, 2002-2006. ScienceDirect
    edited by M.A. Hayat.
    v. 1. Molecular genetics; Lung and breast carcinomas -- v. 2. Molecular pathology; Colorectal carcinoma and prostate carcinoma -- v. 3. Molecular genetics; Liver carcinoma and pancreatic carcinoma -- v. 4. Molecular genetics; Gastrointestinal carcinoma and ovarian carcinoma.
  • 2011 CRCnetBASE
    edited by Leland J. Cseke, Ara Kirakosyan, Peter B. Kaufman, Margaret V. Westfall.
    "Reflecting the milestones in the field that have occurred since the publication of the first edition, this book provides an updated tool kit that incorporates conventional as well as modern approaches to tackling biological and medicinal research in the post genomic era. The authors have evaluated, revised, and sometimes replaced all protocols with more efficient, reliable, or simpler ones. The book is also completely reorganized and covers topics of modern "omics" approaches, including genomics, proteomics, and metabolomics. This is followed coverage of the Manipulation of Biological Systems, including RNAi, and the use of microscopy"--Provided by publisher.
  • 2001 CRCnetBASE
    edited by James F. Willott.
  • 2009 Springer
    editor, Abel Lajtha ; volume editor, Katsuhiko Mikoshiba.
  • 2009 Springer
    Abel Lajtha (ed.) ; volume editors: Daniel C. Javitt and Joshua T. Kantrowitz.
    Alterations of Neurotransmitter Receptors in Schizophrenia: Evidence from Postmortem Studies / L. V. Kristiansen, R. M. Cowell, M. Biscaia, R. E. McCullumsmith and J. H. Meador-Woodruff -- An Overview and Current Perspective on Family Studies of Schizophrenia / L. E. DeLisi -- Auditory Cortex Anatomy and Asymmetry in Schizophrenia / J. F. Smiley -- Cholinergic Mechanisms in Schizophrenia / T. J. Raedler and R. Freedman -- Cortical Dopamine in Schizophrenia / A. Abi-Dargham -- Dysbindin-1 and Its Protein Family With Special Attention to the Potential Role of Dysbindin-1 in Neuronal Functions and the Pathophysiology of Schizophrenia / K. Talbot, W.- Y. Ong, D. J. Blake, J. Tang and N. Louneva, et al. -- Early-Stage Visual Processing Deficits in Schizophrenia / P. D. Butler -- Event-Related Potentials (ERPs) in the Study of Schizophrenia: How Preclinical ERP Studies have Contributed to our Understanding of Schizophrenia / J. M. Phillips, C. R. Maxwell, R. S. Ehrlichman and S. J. Siegel -- G72/G30 in Neuropsychiatric Disorders / J. Shi, L. Cheng, E. S. Gershon and C. Liu -- Glutamatergic Approaches to the Conceptualization and Treatment of Schizophrenia / J. Kantrowitz and D. C. Javitt -- Hippocampus / S. Heckers -- Magnetic Resonance Spectroscopy / P. Milev, S. Miranowski and K. O. Lim -- Neuregulin 1 and Schizophrenia / Y.-J. J. Chen, L. W. Role and D. A. Talmage -- Redox Dysregulation in Schizophrenia: Genetic Susceptibility and Pathophysiological Mechanisms / K. Q. Do, P. Bovet, J. H. Cabungcal, P. Conus and R. Gysin, et al. -- Synaptic Vesicle Associated Proteins and Schizophrenia / H. T. Kao and B. Porton -- The Neurobiology of Negative Symptoms and the Deficit Syndrome / B. A. Fischer, B. Kirkpatrick and W. T. Carpenter -- The Role of Prefrontal Abnormalities in Schizophrenia / M. J. Hoptman and K. A. Nolan.
  • 2015 (30th ed.). AccessMedicine
    29th ed., 2012 AccessMedicine
    Bender, David A.; Botham, Kathleen M.; Granner, Daryl K.; Harper, Harold A.; Kennelly, Peter J.; Mayes, Peter A.; Murray, Robert K.; Rodwell, Victor W.; Weil, P. Anthony.
    Also available: Print – 2003-<2015>
  • 2013 Springer Protocols
    edited by Fernando Vivanco.
    Large-scale characterization of the murine cardiac proteome / Jake Cosme, Andrew Emili, and Anthony O. Gramolini -- Determining protein concentrations of the human ventricular proteome / Arjen Scholten and Albert J.R. Heck -- Multidimensional protein identification technology for direct-tissue proteomics of heart / Dario Di Silvestre, Francesca Brambilla, and Pier Luigi Mauri -- Global protein quantification of mouse heart tissue based on the SILAC Mouse / Anne Konzer [and others] -- Global proteomic profiling and enrichment maps of dilated cardiomyopathy / Ruth Isserlin, Daniele Merico, and Andrew Emili -- Characterization of the human myocardial proteome in dilated cardiomyopathy by label-free quantitative shotgun proteomics of heart biopsies / Elke Hammer, Katrin Darm, and Uwe Völker -- Systems proteomics of healthy and diseased chromatin / Haodong Chen [and others] -- Proteomic analysis of interstitial aortic valve cells acquiring a pro-calcific profile / Millioni Renato [and others] -- Differential protein expression analysis of degenerative aortic stenosis by iTRAQ labeling / Sergio Alonso-Orgaz [and others] -- Characterization of zebrafish cardiac proteome using online pH Gradient SCX-RP HPLC-MS/MS Platform / Jiang Zhang [and others] -- Proteomic analysis of brain mitochondrial proteome and mitochondrial complexes / Ana Lopez-Campistrous and Carlos Fernandez-Patron -- Oxidative modifications of mitochondria complex II / Liwen Zhang [and others] -- Detection of O-GlcNAc modifications on cardiac myofilament proteins / Genaro A. Ramirez-Correa [and others] -- Quantification of mitochondrial S-nitrosylation by CysTMT 6 Switch Assay / Christopher I. Murray [and others] -- Identification of thioredoxin target protein networks in cardiac tissues of a transgenic Mouse / Cexiong Fu [and others] -- Using pure protein to build a multiple reaction monitoring mass spectrometry assay for targeted detection and quantitation / Eric Grote [and others] -- Sequential extraction methodology for cardiac extracellular matrix prior to proteomics analysis / Javier Barallobre-Barreiro [and others] -- Optimized method for identification of the proteomes secreted by cardiac cells / Miroslava Stastna and Jennifer E. Van Eyk -- Secretome of human aortic valves / Fernando de la Cuesta [and others] -- Comparative study of immunodepletion and equalization methods for aortic stenosis human plasma / Felix Gil-Dones [and others].
  • 2013 Springer Protocols
    edited by Magdalena Czader.
    Quantitative BCR-ABL1 RQ-PCR fusion transcript monitoring in chronic myelogenous leukemia / Franklin R. Moore, Carole B. Rempfer, and Richard D. Press -- Detection of BCR-ABL1 kinase domain mutations causing imatinib resistance in chronic myelogenous leukemia / Franklin R. Moore, Fei Yang, and Richard D. Press -- Laboratory detection of JAK2 V617F in human myeloproliferative neoplasms / Jonathan S. Kui, Rosanny Espinal-Witter, and Y. Lynn Wang -- c-kit Mutational analysis in paraffin material / Karl Sotlar -- Detection of recurrent cytogenetic abnormalities in acute lymphoblastic and myeloid leukemias using fluorescence in situ hybridization / Gail H. Vance -- Liquid bead array technology in the detection of common translocations in acute and chronic leukemias / Rodney E. Shackelford [and others] -- Molecular genetic tests for FLT3, NPM1, and CEBPA in acute myeloid leukemia / Qing Zhang, Shaochun Bai, and Gail H. Vance -- Flow cytometric monitoring of residual disease in acute leukemia / Brent L. Wood -- Chimerism analysis following hematopoietic stem cell transplantation / Kathleen M. Murphy -- Detection of clonal immunoglobulin heavy chain gene rearrangements by the polymerase chain reaction and capillary gel electrophoresis / Hongxin Fan and Ryan S. Robetorye -- Detection of clonal T-cell receptor beta and gamma chain gene rearrangement by polymerase chain reaction and capillary gel electrophoresis / Hongxin Fan and Ryan S. Robetorye -- Detection of genetic translocations in lymphoma using fluorescence in situ hybridization / Kaaren K. Reichard and Sheldon Robinett -- Molecular detection of t(14;18)(q32;q21) in follicular lymphoma / C. Cameron Yin and Rajyalakshmi Luthra -- Molecular detection of t(11;14)(q13;q32) in mantle cell lymphoma / C. Cameron Yin and Rajyalakshmi Luthra -- Detection of t(2;5)(p23;q35) in anaplastic large-cell lymphoma by long-range nested polymerase chain reaction assay / C. Cameron Yin and Rajyalakshmi Luthra -- EBER in situ hybridization for Epstein-Barr virus / Lawrence M. Weiss and Yuan-Yuan Chen -- Epstein-Barr virus (EBV) load determination using real-time quantitative polymerase chain reaction / Hongxin Fan and Ryan S. Robetorye -- Molecular and immunohistochemical detection of kaposi sarcoma herpesvirus/human herpesvirus-8 / Amy Chadburn, Janet Wilson, and Y. Lynn Wang -- Detection of cytomegalovirus infection by quantitative polymerase chain reaction / Magdalena Czader, Kristin Post, and Liang Cheng -- Thiopurine S-methyltransferase pharmacogenetics in childhood acute lymphoblastic leukemia / Jun J. Yang and Deepa Bhojwani -- MicroRNA expression profiling using microarrays / Cassandra Love and Sandeep Dave.
  • 2008 Springer Protocols
    edited by Kevin D. Bunting.
    The expanding tool kit for hematopoietic stem cell research / William Tse and Kevin D. Bunting -- Isolation of quiescent murine hematopoietic stem cells by homing properties / Tarja A. Juopperi and Saul J. Sharkis -- Mobilization of hematopoietic stem and progenitor cells in mice / Simon N. Robinson and Ronald P. van Os -- Transmigration of human CD34⁺ cells / Seiji Fukuda and Louis M. Pelus -- Flow cytometry-based cell cycle measurement of mouse hematopoietic stem and progenitor cells / Hongmei Shen, Matthew Boyer, and Tao Cheng -- Analysis of apoptosis in hematopoietic stem cells by flow cytometry / William G. Kerr -- In vitro hematopoietic differentiation of murine embryonic stem cells / Jinhua Shen and Cheng-Kui Qu -- Hematopoietic development of human embryonic stem cells in culture / Xinghui Tian and Dan S. Kaufman -- In vitro human T cell development directed by notch-ligand interactions / Génève Awong, Ross N. La Motte-Mohs, and Juan Carlos Zuñiga-Pflücker -- In vitro assays for cobblestone area-forming cells, LTC-IC, and CFU-C / Ronald P. van Os, Bertien Dethmers-Ausema, and Gerald de Haan -- Hematopoietic stem cell transplant in mice by intra-femoral injection / Yuxia Zhan and Yi Zhao -- Hematopoietic stem cell transplant into non-myeloblated W/Wv mice to detect steady-state engraftment defects / Zhengqi Wang and Kevin D. Bunting -- Isolation and functional characterization of side population stem cells / Jonathan B. Johnnidis and Fernando D. Camargo -- Transplantation of chimeric fetal liver to study hematopoiesis / Sigrid Eckardt and K. John McLaughlin -- Immunodeficient mouse models to study human stem cell-mediated tissue repair / Ping Zhou ... [et al.] -- Retroviral transduction of murine hematopoietic stem cells / Peter Haviernik, Yi Zhang, and Kevin D. Bunting -- Lentiviral gene transduction of mouse and human stem cells / Zhaohui Ye, Xiaobing Yu, and Linzhao Cheng -- Retroviral integration site analysis in hematopoietic stem cells / Olga S. Kustikova, Christopher Baum, and Boris Fehse -- A detailed protocol for bacterial artificial chromosome recombineering to study essential genes in stem cells / Andriy Tsyrulnyk and Richard Moriggl -- Bioluminescence imaging of hematopoietic stem cell repopulation in murine models / Yuan Lin ... [et al.]
  • 2010 Springer Protocols
    edited by Patrick Maurel.
    1. General review on in vitro hepatocyte models and their applications / Christiane Guguen-Guillouzo and Andre Guillouzo -- 2. Human foetal hepatocytes / Anne Weber ... [et al.] -- 3. Isolation and culture of primary hepatocytes from resected human liver tissue / Edward L. LeCluyse and Eliane Alexandre -- 4. Optimisation of the cryopreservation of primary hepatocytes / Nicola J. Hewitt -- 5. Cryopreservation of human hepatocytes for clinical use / Ragai R. Mitry, Sharon C. Lehec, and Robin D. Hughes -- 6. Hepatocyte differentiation / Katy M. Olsavsky Goyak, Elizabeth M. Laurenzana, and Curtis J. Omiecinski -- 7. Reversible manipulation of apoptosis sensitivity in cultured hepatocytes by matrix-mediated manipulation of signaling activities / Patricio Godoy ... [et al.] -- 8. Markers and signaling factors for stem cell differentiation to hepatocytes / Frédéric Lemaigre -- 9. Hepatic stem cells / Robert E. Schwartz and Catherine Verfaillie -- 10. Hepatic stem cells and liver development / Nalu Navarro-Alvarez, Alejandro Soto-Gutierrez, and Naoya Kobayashi -- 11. Generation of hepatocytes from human embryonic stem cells / Neta Lavon -- 12. Isolation and culture of adult human liver progenitor cells / Sabine Gerbal-Chaloin ... [et al.] -- 13. The HepaRG cell line / Marie-Jeanne Marion, Olivier Hantz, and David Durantel -- 14. Transdifferentiation of pancreatic cells to hepatocytes / Chia-Ning Shen and David Tosh -- 15. Evaluation of drug metabolism, drug-drug interactions and in vitro hepatotoxicity with cryopreserved human hepatocytes / Albert P. Li -- 16. The use of human hepatocytes to investigate drug metabolism and CYP enzyme induction / Sylvie Klieber ... [et al.] -- 17. The use of hepatocytes to investigate UDP-glucuronosyltransferases and sulfotransferases / Sylvie Fournel-Gigleux ... [et al.] -- 18. The use of hepatocytes to investigate drug uptake transporters / Kazuya Maeda and Yuichi Sugiyama -- 19. Metabonomic studies on human hepatocyte in primary culture / Vincent Croizmarie ... [et al.] -- 20. The application of HepRG cells in evaluation of cytochrome P450 induction properties of drug compounds / Tommy B. Andersson -- 21. The use of hepatocytes to investigate drug toxicity / María José Gómez-Lechón, José V. Castell, Maria Teresa Donato -- 22. The use of human hepatocytes to investigate bile acid synthesis / Ewa C.S. Ellis and Lisa-Mari Nilsson -- 23. The use of human hepatocytes to investigate blood coagulation factor / Christine Biron-Andréani ... [et al.] -- 24. Use of human hepatocytes to investigate HCV infection / Lydiane Pichard-Garcia ... [et al.] -- 25. The use of hepatocytes to investigate HDV infection / Camille Sureau -- 26. Rodent models of liver repopulation / Helène Gilgenkrantz -- 27. Chimeric mice with humanized liver / Stephen C. Strom, Julio Davila, and Markus Grompe -- 28. Bioartificial liver support systems / Gesine Pless -- 29. Human hepatocyte transplantation / Anil Dhawan ... [et al.].
  • 2007
    Ronald W. Dudek.
    Status: Not Checked OutLane Catalog Record
  • 2013 Springer Protocols
    edited by Michael J. Wheeler.
    Hormone measurement is necessary for the diagnosis of a wide range of clinical conditions and is essential for monitoring the effectiveness of treatment. As the number of hormone requests in the clinical field rises exponentially, it has become imperative to create hormone assays accessible to researchers with a varied range of equipment. Hormone Assays in Biological Fluids, Second Edition reviews common techniques used to measure hormones as well as relatively new methods such as tandem mass spectrometry. Additionally, subsequent chapters detail methods for a broad range of hormones; Techniques and principles covered are transferable to a wide range of substances across species. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Hormone Assays in Biological Fluids, Second Edition will serve students, technologists, laboratory scientists, and researchers looking to apply, or attain a greater understanding of, methods for measuring hormones.
  • 2006 Springer Protocols
    edited by Michael J. Wheeler, J.S. Morley Hutchinson.
    Immunoassay techniques / Michael J. Wheeler -- High-performance liquid chromatography for hormone assay / John W. Honour -- Gas chromatography-mass spectrometry / John W. Honour -- An introduction to PCR techniques / Gill Rumsby -- Methods for the investigation of thyroid function / Christine R. Squire -- Assays for LH, FSH, and prolactin / Michael J. Wheeler -- Adrenocorticotropic hormone and related peptides / Les A. Perry -- Measurement of glucocorticoids in biological fluids / Geoff Holder -- Urinary steroid profiling / Norman F. Taylor -- Measurement of aldosterone in blood / Marion L. Cawood -- Measurement of plasma renin activity / Marion L. Cawood -- Measurement of androgens / Michael J. Wheeler -- Measurement of gut hormones in plasma / Shahred Taheri, Mohammed Ghatei, and Steve Bloom -- Measurement of melatonin and 6-sulphatoxymelatonin / Benita Middleton.
  • 2009 Springer
    editor, Suzanne A.W. Fuqua.
    Also available: Print – 2009
  • 2014 Springer Protocols
    edited by Yacine Graba, René Rezsohazy.
    Discovery and classification of homeobox genes in animal genomes / Ferdinand Marlétaz, Jordi Paps, Ignacio Maeso, and Peter W. H. Holland -- How to study hox gene expression and function in mammalian oocytes and early embryos / Delphine Paul, Caroline Sauvegarde, René Rezsohazy, and Isabelle Donnay -- Genetic lineage tracing analysis of anterior hox expressing cells / Brigitte Laforest, Nicolas Bertrand, and Stéphane Zaffran -- A genetic strategy to obtain P-Gal4 elements in the Drosophila hox genes / Luis de Navas, David Foronda, Delia del Saz, and Ernesto Sánchez-Herrero -- Hox complex analysis through BAC recombineering / Mark Parrish, Youngwook Ahn, Christof Nolte, Bony De Kumar and Robb Krumlauf -- The genetics of murine Hox loci : TAMERE, STRING, and PANTHERE to engineer chromosome variants / Patrick Tschopp and Denis Duboule -- Topological organization of Drosophila hox genes using DNA fluorescent in situ hybridization / Frédéric Bantignies and Giacomo Cavalli -- Mining the Cis-regulatory elements of hox clusters / Navneet Kaur Matharu and Rakesh K. Mishra -- Functional analysis of hox genes in Zebrafish / Franck Ladam and Charles G. Sagerström -- Transgenesis in non-model organisms : the case of Parhyale / Zacharias Kontarakis and Anastasios Pavlopoulos -- Tissue specific RNA isolation in Drosophila embryos : a strategy to analyze context dependent transcriptome landscapes using FACS / Arnaud Defaye and Laurent Perrin -- Hox transcriptomics in Drosophila embryos / Maria Polychronidou and Ingrid Lohmann -- Measuring hox-DNA binding by electrophoretic mobility / Kelly Churion [and four others] -- Chromatin immunoprecipitation and chromatin immunoprecipitation with massively parallel sequencing on mouse embryonic tissue / Shilu Amin and Nicoletta Bobola -- chIP for hox proteins from Drosophila imaginal discs / Pavan Agrawal and L. S. Shashidhara -- SELEX-seq : a method for characterizing the compete repertoire of binding site preferences for transcription factor complexes / Todd R. Riley [and six others] -- DamID as an approach to studyinhg long-distance chromatin iteractions / Fabienne Cléard, François Karch, and oRbert K. Maeda -- cgCHIP : a cell type- and gene-specific method for chromatin analysis / Marios Agelopoulos, Daniel J. McKay, and Ricahrd S. Mann -- Bimolecular fluorescence complementation (biFC) in live Drosophila embryos / Marilyne Duffraisse, Bruno Hudry, and Samir Merabet -- Rational drug repurposing using sscMap analysis in a HOX-TALE model of leukemia / Laura M. Kettyle, Fabio G. Liberante, and Alexander Thompson.
  • 2002 CRCnetBASE
    edited by Karen M. Gooding, Fred E. Regnier.
  • 2011
    Tom Strachan and Andrew Read.
    Status: Not Checked OutLane Catalog Record
    Nucleic acid structure and gene expression -- Chromosome structure and function -- Genes in pedigrees and populations -- Cells and cell-cell communication -- Principles of development -- Amplifying DNA : cell-based DNA cloning and PCR -- Nucleic acid hybridization : principles and applications -- Analyzing the structure and expression of genes and genomes -- Organization of the human genome -- Model organisms, comparative genomics, and evolution -- Human gene expression -- Studying gene function in the post-genome era -- Human genetic variability and its consequences -- Genetic mapping of mendelian characters -- Mapping genes conferring susceptibility to complex diseases -- Identifying human disease genes and susceptibility factors -- Cancer genetics -- Genetic testing of individuals -- Pharmacogenetics, personalized medicine and population screening -- New approaches to therapy -- Genetic manipulation of animals for modeling disease and investigating gene function -- Genetic approaches to treating disease.
  • 2014 Springer Protocols
    edited by Michael Steinitz.
    Human monoclonal antibodies : the residual challenge of antibody immunogenicity / Herman Waldmann -- Technical and ethical limitations in making human monoclonal antibodies (an overview) / Mark C. Glassy and Rishab Gupta -- Therapeutic human monoclonal antibodies in inflammatory diseases / Sotirios Kotsovilis and Evangelos Andreakos -- Therapeutic human monoclonal antibodies against cancer / Jamie Jarboe, Anumeha Gupta, and Wasif Saif -- Polyclonal and monoclonal antibodies in clinic / Bharath Wootla, Aleksandar Denic, and Moses Rodriguez -- Production of human monoclonal Antibodies by the Epstein-Barr virus method / Michael Steinitz -- Humanization and simultaneous optimization of monoclonal antibody / T. Kuramochi, T. Igawa, H. Tsunoda, and K. Hattori -- Chimeric Antibodies / Kohei Kurosawa, Waka Lin, and Kunihiro Ohta -- Recombinant genetic libraries and human monoclonal antibodies / Jarrett J. Adams, Bryce Nelson, and Sachdev S. Sidhu -- Production of stabilized scFv antibody fragments in the E. coli bacterial cytoplasm / Lilach Vaks and Itai Benhar -- Construction and production of an IgG-like tetravalent bispecific antibody, IgG-single-chain Fv fusion / Dan Lu and Zhenping Zhu -- Construction of human antibody gene libraries and selection of antibodies by phage display / André Frenzel [and others] -- Antigen-specific human monoclonal antibodies from transgenic mice / Susana Magadán Mompó and África González-Fernández -- Phage display technology for human monoclonal antibodies / Cecilia Deantonio [and others] -- Antigen-specific in vitro immunization : a source for human monoclonal antibodies / Kosuke Tomimatsu and Sanetaka Shirahata -- Methods for radiolabelling of monoclonal antibodies / Vladimir Tolmachev, Anna Orlova, and Karl Andersson -- Purification of human monoclonal antibodies and their fragments / Thomas Müller-Späth and Massimo Morbidelli -- Idiotype-specific intravenous immunoglobulin (IVIG) for therapy of autoimmune diseases / Miri Blank, Tomer Bashi, and Yehuda Shoenfeld.
  • 2005 Springer Protocols
    edited by Tuofu Zhu.
  • 2012 ScienceDirect
    edited by P. Michael Conn.
    Section 1. Viruses, microbes, and parasites. Microbial cells analysis by atomic force microscopy / David Alsteens ; Understanding parasite transmission through imaging approaches / Mirko Singer and Freddy Frischknecht ; Multiphoton microscopy applied for real-time intravital imaging of bacterial infections in vivo / Ferdinand X. Choong ... [et al.] ; Analysis of virus entry and cellular membrane dynamics by single particle tracking / Helge Ewers and Mario Schelhaas ; Imaging of live malaria blood stage parasites / Christof Grüring and Tobias Spielmann ; Escherichia coli K1 invasion of human brain microvascular endothelial cells / Lip Nam Loh and Theresa H. Ward -- Section 2. Disease states. Real-time imaging after cerebral ischemia : model systems for visualization of inflammation and neuronal repair / Pierre Cordeau and Jasna Kriz ; In vivo real-time visualization of leukocytes and intracellular hydrogen peroxide levels during a zebrafish acute inflammation assay / Luke Pase, Cameron J. Nowell, and Graham J. Lieschke ; Imaging protein oligomerization in neurodegeneration using bimolecular fluorescence complementation / Federico Herrera, Susana Gonçalves, and Tiago Fleming Outeiro ; 3D/4D functional imaging of tumor-associated proteolysis : impact of microenvironment / Kamiar Moin ... [et al.] -- Section 3. Other techniques. Live cell imaging in live animals with fluorescent proteins / Robert M. Hoffman ; Protein activation dynamics in cells and tumor micro arrays assessed by time resolved Förster resonance energy transfer / Véronique Calleja, Pierre Leboucher, Banafshe ́Larijani ; Imaging stem cell differentiation for cell-based tissue repair / Zhenghong Lee ... [et al.] ; Understanding the initiation of B cell signaling through live cell imaging / Angel M. Davey ... [et al.] ; A quantitative method for measuring phototoxicity of a live cell imaging microscope / Jean-Yves Tinevez ... [et al.] ; High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines / Davide Danovi ... [et al.] ; High-throughput screening in primary neurons / Punita Sharma ... [et al.] ; Live imaging fluorescent proteins in early mouse embryos / Panagiotis Xenopoulos, Sonja Nowotschin, and Anna-Katerina Hadjantonakis ; Methods for three-dimensional analysis of dendritic spine dynamics / Enni Bertling ... [et al.] ; Imaging cell competition in Drosophila imaginal discs / Shizue Ohsawa ... [et al.] ; Live cell imaging of the oviduct / Sabine Kölle -- Computational quantification of fluorescent leukocyte numbers in zebrafish embryos / Felix Ellett and Graham J. Lieschke -- Four-dimensional tracking of lymphocyte migration and interactions in lymph nodes by two-photon microscopy / Masahiro Kitano and Takaharu Okada.
  • 2001 CRCnetBASE
    edited by Xavier Ronot, Yves Usson.
  • 2013 Springer
    Igor Malyshev.
    A General Description of HSPs, The Molecular Structure of HSP70 and The HSP70 Cycle -- The Functions of HSP70 in Normal Cells -- HSP70 in Damaged Cells -- Mechanisms of Activation and Inactivation of HSP70 Synthesis -- HSP70 in the Immune Responses -- HSP70 in Carcinogenesis -- HSP70 in aging -- The role of HSP70 in the protection of: (A) The brain in alzheimer's disease and (B) The heart in cardiac surgery.
  • 2013 Springer Protocols
    edited by Kelly M. Fulton, Susan M. Twine.
    Introduction to the immune system / Scott McComb ... [et al.] -- Immunoproteomics : current technology and applications / Kelly M. Fulton and Susan M. Twine -- Antigen identification using SEREX / Ugur Sahin and Özlem Türeci -- Antigen discovery using whole-genome phage display libraries / Elisa Beghetto and Nicola Gargano -- Methods and applications of serological proteome analysis / Kelly M. Fulton ... [et al.] -- Pre-absorbed immunoproteomics : a novel method for the detection of bacterial surface proteins / Guangjin Liu, Wei Zhang, and Chengping Lu -- Identification of the antigen content of electroimmunoprecipitates / N. Helena Beyer and Niels H.H. Heegaard -- Immunoproteomics approach to screen the antigenicity of the influenza virus / Kevin M. Downard -- In vivo microbial antigen discovery (InMAD) to identify diagnostic proteins and polysaccharides that are circulating during microbial infections / Sindy J. Chaves ... [et al.] -- Chemo-enzymatic production of O-glycopeptides for the detection of serum glycopeptide antibodies / Alexander Nøstdal and Hans H. Wandall -- Enrichment and characterization of glycopeptide epitopes from complex mixtures / Luc Tessier, Kelly M. Fulton, and Susan M. Twine -- Whole-cell MALDI-TOF mass spectrometry : a tool for immune cell analysis and characterization / Richard Ouedraogo ... [et al.] -- Cell-based arrays for the identification of interacting polypeptide domains or epitopes / Richard H. Maier, Christina J. Maier, and Kamil Önder -- Identification and quantitation of MHC class II-bound peptides from mouse spleen dendritic cells by immunoprecipitation and mass spectrometry analysis / Leonia Bozzacco and Haiqiang Yu -- Construction and screening of an antigen-derived peptide library displayed on yeast cell surface for CD4+ T cell epitope identification / Fei Wen and Huimin Zhao -- Profiling of cytokine and chemokine responses using multiplex bead array technology / Greg Harris and Wangxue Chen -- Preparation of the low molecular weight serum proteome for mass spectrometry analysis / Timothy J. Waybright ... [et al.] -- Identification of protein biomarkers in human serum using iTRAQ and shotgun mass spectrometry / Theodoros A. Koutroukides ... [et al.] -- Genome-based bioinformatic prediction of major histocompatibility complex (MHC) epitopes / Simon J. Foote -- Structure-based prediction of major histocompatibility complex (MHC) epitopes / Andrew J. Bordner -- Prioritization of therapeutic targets of inflammation using proteomics, bioinformatics, and in silico cell-cell interactomics / Arsalan S. Haqqani and Danica B. Stanimirovic -- Commercial considerations for immunoproteomics / Scott M. Ferguson.
  • 2013 Springer Protocols
    edited by Maria Victoria Schneider.
    Systems biology can now be considered an established and fundamental field in life sciences. It has moved from the identification of molecular 'parts lists' for living organisms towards synthesising information from different 'omics'-based approaches to generate and test new hypotheses about how biological systems work. In Silico Systems Biology: Methods and Protocols, expert researchers in the field detail a practical set of chapters based often on actual materials used and develop for face-to-face training with examples and case studies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, step-by-step workflows, and key tips on troubleshooting and avoiding known pitfalls. -- Publisher website.
  • 2001 CRCnetBASE
    Gerard Morel, Annie Cavalier, Lynda Williams.
  • 2015 Springer Protocols
    edited by Giselbert Hauptmann.
    Optimization of in situ hybridization and co-expression analysis in the central nervous system / Louisa Mezache ... [et al.] -- In situ hybridization methods for RNA visualization in C. elegans / Gina Broitman-Maduro and Morris F. Maduro -- Multicolored visualization of transcript distributions in Drosophila embryos / Iris Söll and Giselbert Hauptmann -- Exploring brain genoarchitecture by single and double chromogenic in situ hybridization (ISH) and immunohistochemistry (IHC) in whole-mount embryos / José Luis ... [et al.] -- Exploring brain genoarchitecture by single and double chromogenic in situ hybridization (ISH) and immunohistochemistry (IHC) on cryostat, paraffin, or floating sections / José Luis Ferran ... [et al.] -- Combined radioactive and nonradioactive double in situ hybridization (DISH) / Jong-Hyun Son and Ursula H. Winzer-Serhan -- One FISH, dFISH, three FISH : sensitive methods of whole- mount fluorescent in situ hybridization in freshwater planarians / David D. R. Brown and Bret J. Pearson -- Whole-mount in situ hybridization and immunohistochemistry in xenopus embryos / Edgar M. Pera ... [et al.] -- Manual and automated whole-mount in situ hybridization for systematic gene expression analysis in embryonic Zebrafish forebrain / Iris Söll and Giselbert Hauptmann -- Large-scale ISH on mouse brain sections for systematic gene expression analysis in developing mouse diencephalon / Tomomi Shimogori and Seth Blackshaw -- High-throughput in situ hybridization : systematical production of gene expression data and beyond / Lars Geffers and Gregor Eichele -- Analysis of embryonic gene expression patterns in the hagfish / Yasuhiro Oisi ... [et al.] -- Gene expression analysis of lamprey embryos / Fumiaki Sugahara, Yasunori Murakami, and Shigeru Kuratani -- Detection of mRNA and microRNA expression in basal chordates, amphioxus and ascidians / Simona Candiani, Greta Garbarino, and Mario Pestarino -- Analysis of gene expression in neurons and synapses by multi-color in situ hybridization / Leonid L. Moroz and Andrea B. Kohn -- Fluorescent in situ hybridization in primary hippocampal neurons to detect localized mRNAs / Jacki E. Heraud-Farlow, Tejaswini Sharangdhar, and Michael A. Kiebler -- Combining whole-mount in situ hybridization with neuronal tracing and immunohistochemistry / Jeremy S. Duncan ... [et al.] -- Fluorescent in situ hybridization detection of microRNAs in Newt tissue cryosections / Jamie Ian Morrison -- Subcellular transcript localization in Drosophila embryos and tissues visualized by multiplex-FISH / Julie Bergalet ... [et al.] -- Application of alkaline phosphatase-mediated azo dye staining for dual fluorescent in situ hybridization in Zebrafish / Giselbert Hauptmann, Gilbert Lauter, and Iris Söll -- Multiplex fluorescent RNA in situ hybridization via RNAscope / Hongwei Wang ... [et al.] -- Quantum dot-based in situ hybridization and immunohistochemistry to detect mRNA and protein at subcellular levels, comparison with studies using electron microscopy / Akira Matsuno ... [et al.] -- Quantitative multiplexed quantum dot based in situ hybridization in formalin-fixed paraffin-embedded tissue / Eleni Tholouli, Judith A. Hoyland, and Richard J. Byers -- Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in xenopus / Andriani Ioannou ... [et al.] -- Quantitative single-molecule mRNA fluorescent in situ hybridization in C. elegans / Remco A. Mentink ... [et al.] -- In situ detection of individual mRNA molecules with padlock probes and target-primed rolling-circle amplification in fixed mouse brain tissues / Thomas Hauling ... [et al.] -- Extended multiplexed fluorescent in situ hybridization by combinatorial encoding of individual transcripts / Lars Martin Jakt and Satoko Moriwaki -- Characterizing cellular identity at one cell resolution / Amaresh K. Ranjan, Mugdha V. Joglekar, and Anandwardhan A. Hardikar -- Transglutaminase-mediated in situ hybridization (TransISH) for mRNA detection in mammalian tissues / Katsuyuki Miyawaki, Sumihare Noji, and Noriho Kamiya -- ECHO-FISH for gene transcript detection in neuronal and other cells and subcellular compartments / Dan Ohtan Wang and Akimitsu Okamoto.
  • 2014 Springer Protocols
    edited by Boye Schnack Nielsen, Bioneer A/S, Molecular Histology, Hørholm, Denmark.
    "In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions."--Page [4] of cover.
  • 2005 Springer Protocols
    edited by Ian A. Darby, Tim D. Hewitson.
    In situ hybridization using riboprobes on free-floating brain sections / Neil C. Owens, F. Martin Hess, and Emilio Badoer -- An in situ hybridization technique to detect low-abundance slug mRNA in adherent cultured cells / Changsun Choi ... [et al.] -- Identification of transplanted human cells in animal tissues / Daniel Benten, Kang Cheng, and Sanjeev Gupta -- In situ hybridization to plant tissues and chromosomes / Andreas Houben, Sharon J. Orford, and Jeremy N. Timmis -- Histochemical localization of cell proliferation using in situ hybridization for histone mRNA / Tim D. Hewitson, Kristen J. Kelynack, and Ian A. Darby -- Histochemical localization of apoptosis with in situ labeling of fragmented DNA / Tim D. Hewitson, Teresa Bisucci, and Ian A. Darby -- Nonradioactive in situ hybridization on frozen sections and whole mounts / Julia Asp, Alexandra Abramsson, and Christer Betsholtz -- In situ hybridization of whole mount embryos / Murray Hargrave, Josephine Bowles, and Peter Koopman -- In situ detection of Epstein-Barr virus and phenotype determination of EBV-infected cells / Gerald Niedobitek and Hermann Herbst -- PNA- situ hybridization method for detection of HIV-1 DNA in virus-infected cells and subsequent detection of cellular and viral proteins / Tomoko Hagiwara, Junko Hattori, and Tsuguhiro Kaneda -- Electron microscopy and in situ hybridization: expression of P2Y2 receptor mRNA in the cerebellum / Andrzej Loesch and Rainer Glass -- The use of combined immunohistochemical labeling and in situ hybridization to colocalize mRNA and protein in tissue sections / Malcolm D. Smith, Michael Ahern, and Mark Coleman -- Semiquantitative in situ hybridization using radioactive probes to study gene expression in motoneuron populations / Paul D. Storer and Tracey DeLucia -- Quantitative in situ hybridization of tissue microarrays / Adrian M. Jubb ... [et al.]. Treatment of tissue sections for in situ hybridization / Gregory H. Tesch, Hui Y. Lan, and David J. Nikolic-Paterson -- Preparation of template DNA and labeling techniques / Peter J. Roche -- In situ hybridization using cRNA probes: isotopic and nonisotopic detection methods / Ian A. Darby ... [et al.] -- Tyramide signal amplification for DNA and mRNA in situ hybridization / Ernst J.M. Speel, Anton H.N. Hopman, and Paul Komminoth -- Expression analysis of murine genes using in situ hybridization with radioactive and nonradioactively labeled RNA probes / Anne Chotteau-Lelièvre, Pascal Dollé, and Françoise Gofflot --
  • 2007 Springer Protocols
    edited by Guido Grandi.
    Energy systems for ATP regeneration in cell-free protein synthesis reactions / Kara A. Calhoun and James R. Swartz -- Continuous-exchange protein-synthesizing systems / Vladimir A. Shirokov ... [et al.] -- Cell-free production of integral membrane proteins on a preparative scale / Christian Klammt ... [et al.] -- SIMPLEX : a novel method for high-throughput construction and screening of protein libraries / Suang Rungpragayphan, Tsuneo Yamane, and Hideo Nakano -- Methods for high-throughput materialization of genetic information based on the wheat germ cell-free expression system / Tatsuya Sawasaki ... [et al.] -- Exogenous protein expression in Xenopus oocytes : basic procedures / Elena Bossi ... [et al.] -- In vitro translation to study HIV protease activity / Zene Matsuda ... [et al.] -- The protein truncation test in mutation detection and molecular diagnosis / Oliver Hauss and Oliver Muller -- Creation of novel enantioselective lipases by SIMPLEX / Yuichi Koga, Tsuneo Yamane, and Hideo Nakano -- In vitro transcription and translation coupled with two-dimensional electrophoresis for bacterial proteome analysis / Nathalie Norais ... [et al.] -- In vitro screen of bioinformatically selected Bacillus anthracis vaccine candidates by coupled transcription, translation, and immunoprecipitation analysis / Orit Gat, Haim Grosfeld, and Avigdor Shafferman -- Functional expression of Type 1 rat GABA transporter in microinjected Xenopus laevis oocytes / Stefano Giovannardi ... [et al.] -- Production of protein for nuclear magnetic resonance study using the wheat germ cell-free system / Toshiyuki Kohno and Yaeta Endo.
  • 2014 Springer Protocols
    edited by Hans-Joachim Anders, Adriana Migliorini.
    Detection of RNA modifications by HPLC analysis and competitive ELISA / Gernot Nees, Andreas Kaufmann, and Stefan Bauer -- Enzymatic synthesis and purification of a defined RIG-I ligand / Marion Goldeck ... [et al.] -- Crystallization of mouse RIG-I ATPase domain : in situ proteolysis / Filiz Civril and Karl-Peter Hopfner -- Isolation of RIG-I-associated RNAs from virus-infected cells / Andreas Schmidt ... [et al.] -- Structure modeling of Toll-Like Receptors / Jing Gong and Tiandi Wei -- Nucleic acid recognition in dendritic cells / Alexander Heiseke, Katharina Eisenächer, and Anne Krug -- Viral nucleic acid recognition in human nonimmune cells : in vitro systems / Andrea Ribeiro and Markus Wörnle -- Analysis of nucleic acid-induced nonimmune cell death in vitro / Simone Romoli and Adriana Migliorini -- In vitro analysis of nucleic acid recognition in B lymphocytes / Saskia Ziegler and Isabelle Bekeredjian-Ding -- Mapping of optimal CD8 T cell epitopes / Julia Roider, Thomas Vollbrecht, and Rika Draenert -- Modular approach to suppression assays : TLR ligands, conditioned medium, and viral infection / Viktor H. Koelzer and David Anz -- MicroRNA methodology : advances in miRNA technologies/ Theresa Kaeuferle ... [et al.] -- Expression profiling by real-time quantitative polymerase chain reaction (RT-qPCR) / Maciej Lech and Hans-Joachim Anders -- Evaluating the role of nucleic acid antigens in murine models of systemic lupus erythematosus / Amanda A. Watkins, Ramon G. B. Bonegio, and Ian R. Rifkin -- Induction and analysis of nephrotoxic serum nephritis in mice / John M. Hoppe and Volker Vielhauer -- Isolation of intratumoral leukocytes of TLR-stimulated tumor-bearing mice / Moritz Rapp, David Anz, and Max Schnurr -- Bifunctional siRNAs for tumor therapy / Fanny Matheis and Robert Besch.
  • 2009 Springer Protocols
    edited by Christopher J. Barker.
    The role of inositol and the principles of labelling, extraction, and analysis of inositides in mammalian cells / Christopher J. Barker and Per-Olof Berggren -- HPLC separation of inositol polyphosphates / Christopher J. Barker, Christopher Illies, and Per-Olof Berggren -- Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in mammalian cells / James C. Otto and John D. York -- A femtomole-sensitivity mass assay for inositol hexakisphosphate / Andrew J. Letcher, Michael J. Schell, and Robin F. Irvine -- Synthesis of InsP₇ by the inositol hexakisphosphate kinase 1 (IP6K1) / Cristina Azevedo ... [et al.] -- Protein pyrophosphorylation by diphosphoinositol pentakisphosphate (InsP₇) / J. Kent Werner Jr., Traci Speed, and Rashna Bhandari -- Synthesis and nonradioactive micro-analysis of diphosphoinositol phosphates by HPLC with postcolumn complexometry / Hongying Lin, Karsten Lindner, and Georg W. Mayr -- Diphosphosinositol polyphosphates and energy metabolism : assay for ATP/ADP ratio / Andreas Nagel ... [et al.] -- Isolation of inositol 1,4,5-triphosphate receptor-associating proteins and selective knockdown using RNA interference / Akihiro Mizutani ... [et al.] -- Phosphoinositide-specific phospholipase C [beta]1 signal transduction in the nucleus / Roberta Fiume ... [et al.] -- Methods to assess changes in the pattern of nuclear phosphoinositides / Nullin Divecha -- Measurement of phosphoinositide 3-kinase products in cultured mammalian cells by HPLC / Frank T. Cooke -- Phosphoinositide analysis by liquid chromatography-mass spectrometry / Trevor R. Pettitt -- Imaging phosphoinositide dynamics in living cells / Anne Wuttke, Olof Idevall-Hagren, and Anders Tengholm.
  • 2005 Springer
    edited by Wolfgang Walz.
  • 2006 ScienceDirect
    edited by John T. Romeo.
    Terpene indole alkaloid biosynthesis / Sarah E. O'Connor, Elizabeth McCoy -- Pathways to diverse ergot alkaloid profiles in fungi / Daniel G. Panaccione, Christopher L. Schardl, Christine M. Coyle -- Compartmentalization of plant secondary metabolism / Nailish Samanani, Peter J. Facchini -- Comparative genomics of the shikimate pathways in Arabidopsis, Populus trichocarpa and Oryza sativa : shikimate pathway gene family structure and identification of candidates for missing links in pehnylalanine biosynthesis / Björn Hamberger ... [et al.] -- Tomato glandular trichomes as a model system for exploring evolution of specialized metabolism in a single cell / Eyal Fridman ... [et al.] -- Anacardic acid biosynthesis and bioactivity / David J. Schultz, Nalinie S. Wickramasinghe, Carolyn M. Klinge -- Molecular and biochemical investigations of sorgoleone biosynthesis / Daniel Cook ... [et al.] -- Biosynthesis of terpenophenolic metabolites in hop and cannabis / Jonathan E. Page, Jana Nagel -- Engineering pathway enzymes to understand the function and evolution of sterol structure and activity / Pruthvi Jayasimha ... [et al.] -- Methylation and demethylation of plant signaling molecules / Yue Yang ... [et al.] -- Recent advances in auxin biosynthesis and conjugation / Amber Kei Bowers, Yunde Zhao -- Auxin biology and biosynthesis / Jessica Calio, Yuen Yee Tam, Jennifer Normanly -- Translational opportunities in plant biochemistry / Cecilia A. McIntosh.
  • 2010 Springer
    edited by Andrea Becchetti, Annarosa Arcangeli.
    Integrin structure and functional relation with ion channels / Annarosa Arcangeli and Andrea Becchetti -- Introduction to ion channels / Chiara Di Resta and Andrea Becchetti -- Biochemical methods to study the interactions between integrins and ion channels / Olivia Crociani -- Optical methods in the study of protein : protein interactions / Alessio Masi ... [et al.] -- Integrins and signal transduction / -- Sara Cabodi ... [et al.] -- Physical and functional interaction between integrins and herg1 channels in cancer cells / Serena Pillozzi and Annarosa Arcangeli -- Coordinated regulation of vascular Ca2+ and K+ channels by integrin signaling / Peichun Gui ... [et al.] -- Adhesion : dependent modulation of macrophage K+ channels / Margaret Colden-Stanfield -- Integrin receptors and ligand : gated channels / Raffaella Morini and Andrea Becchetti -- Integrins and ion channels in cell migration : implications for neuronal development, wound healing and metastatic spread / Andrea Becchetti and Annarosa Arcangeli.
    Also available: Print – 2010
  • 2001. ScienceDirect
    edited by Mark P. Mattson.
  • 2013 ScienceDirect
    edited by Robin Ganellin, Stanley Roberts, Roy Jefferis.
    Chapter 1. Introduction to enzymes, receptors and the action of small molecule drugs -- chapter 2. Protein structure and function -- chapter 3. The small molecule drug discovery process : from target selection to candidate selection -- chapter 4. Protein therapeutics (introduction to biopharmaceuticals) -- chapter 5. Similarities and differences in the discovery and use of biopharmaceuticals and small-molecule chemotherapeutics -- chapter 6. Therapies for type 2 diabetes : modulating the incretin pathway using small molecule peptidase inhibitors or peptide mimetics -- chapter 7. The structure and business of biopharmaceutical companies including the management of risks and resources -- chapter 8. Discovery and development of the anticancer agent gefitinib, an inhibitor of the epidermal growth factor receptor tyrosine kinase -- chapter 9. Targeting HER2 by monoclonal antibodies for cancer therapy -- chapter 10. Recombinant human erythropoietin and its analogues -- chapter 11. Lysosomal storage disorders : current treatments and future directions -- chapter 12. Hormone replacement therapy -- chapter 13. Design of the anti-HIV protease inhibitor darunavir -- chapter 14. The case of anti-TNF agents -- chapter 15. Discovery of the cholesterol absorption inhibitor, ezetimibe.
  • 2013 ScienceDirect
    William Stillwell.
    An Introduction to Biological Membranes: From Bilayers to Rafts covers many aspects of membrane structure/function that bridges membrane biophysics and cell biology. Offering cohesive, foundational information, this publication is valuable for advanced undergraduate students, graduate students and membranologists who seek a broad overview of membrane science. Brings together different facets of membrane research in a universally understandable mannerEmphasis on the historical development of the field. Topics include membrane sugars, membrane models, membrane isolation methods, and membrane transport.
  • 2012
    Anthony J.F. Griffiths, Susan R. Wessler, Sean B. Carroll, John Doebley.
    Status: Not Checked OutLane Catalog Record
    1. The Genetics Revolution in the Life Sciences -- pt. I. TRANSMISSION GENETICS -- 2. Single-Gene Inheritance -- 3. Independent Assortment of Genes -- 4. Mapping Eukaryote Chromosomes by Recombination -- 5. The Genetics of Bacteria and Their Viruses -- 6. Gene Interaction -- pt. II. FROM DNA TO PHENOTYPE -- 7. DNA: Structure and Replication -- 8. RNA: Transcription and Processing -- 9. Proteins and Their Synthesis -- 10. Gene Isolation and Manipulation -- 11. Regulation of Gene Expression in Bacteria and Their Viruses -- 12. Regulation of Gene Expression in Eukaryotes -- 13. The Genetic Control of Development -- 14. Genomes and Genomics -- pt. III. MUTATION, VARIATION, AND EVOLUTION -- 15. The Dynamic Genome: Transposable Elements -- 16. Mutation, Repair, and Recombination -- 17. Large-Scale Chromosomal Changes -- 18. Population Genetics -- 19. The Inheritance of Complex Traits -- 20. Evolution of Genes and Traits.
  • 2014 Springer
    Gregory R. Bowman, Frank Noé, Vijay S. Pande, editors.
    The aim of this book volume is to explain the importance of Markov state models to molecular simulation, how they work, and how they can be applied to a range of problems. The Markov state model (MSM) approach aims to address two key challenges of molecular simulation: 1) How to reach long timescales using short simulations of detailed molecular models 2) How to systematically gain insight from the resulting sea of data MSMs do this by providing a compact representation of the vast conformational space available to biomolecules by decomposing it into states sets of rapidly interconverting conformations and the rates of transitioning between states.This kinetic definition allows one to easily vary the temporal and spatial resolution of an MSM from high-resolution models capable of quantitative agreement with (or prediction of) experiment to low-resolution models that facilitate understanding. Additionally, MSMs facilitate the calculation of quantities that are difficult to obtain from more direct MD analyses, such as the ensemble of transition pathways. This book introduces the mathematical foundations of Markov models, how they can be used to analyze simulations and drive efficient simulations, and some of the insights these models have yielded in a variety of applications of molecular simulation.
    Also available: Print – 2014
  • 2003 CRCnetBASE
    Jack Tuszynski, Michal Kurzynski.
  • 2014 CRCnetBASE
    edited by Matthew Eckwahl, MPhil.
    Part 1. Cell polarity, growth, and cell cycle -- part 2. Yeast regulatory circuits -- part 3. Gene expression and trait locus analysis -- part 4. Comparative genomics.
  • 2006 Springer Protocols
    edited by James D. Stockand, Mark S. Shapiro.
    A simple in-vivo method for assessing changes of membrane bound ion channel density in xenopus oocytes / Mouhamed S. Awayda ... [et al.] -- Preparation of cortical brain slices for electrophysiological recording / Costa M. Colbert -- Juxtacellular labeling and chemical phenotyping of extracellulary recorded neurons in vivo / Glenn M. Toney and Lynette C. Daws -- Carbon fiber amperometry in the study of ion channels and secretion / Duk-Su Koh -- Genetic screening for functionality of bacterial potassium channel mutants using K+ update-deficient escherichia coli / Lyubov V. Parfenova and Brad S. Rothberg -- KCNQ1 K channel-mediated cardiac channelopathies / Gildas Loussouarn, Isabelle Baro, and Denis Escade -- Tissue-specific transgenic and knockout mice / Andrée Porret ... [et al.]. Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system / Alexander Staruschenko ... [et al.] -- Overexpression of proteins in neurons using replication-deficient virus / Richard M. Ahlquist and Jane M. Sullivan -- Exogenous expression of proteins in neurons using the biolistic particle delivery system / Nikita Gamper and Mark S. Shapiro -- Tertiary and quaternary structure formation of voltage-gated potassium channels / John M. Robinson, Andrey Kosolapov, and Carol Deutsch -- Biophysical approach to determine the subunit stoichiometry of the epithelial sodium channel using the xenopus laevis oocyte expression system / Farhad Kosari, Shaou Sheng, and Thomas R. Kleyman -- Spectroscopy-based quantitative fluorescence resonance energy transfer analysis / Jie Zheng -- Probing the effects of phosphoinositides on ion channels / Chou-Long Huang -- Epithelial sodium channel in planar lipid bilayers / Bakhrom K. Berdiev and Dale J. Benos --
  • 2013 Springer Protocols
    edited by Alain Goossens and Laurens Pauwels.
    Phenotyping jasmonate regulation of senescence / Martin A. Seltmann and Susanne Berger -- Characterizing jasmonate regulation of male fertility in Arabidopsis / Bryan Thines, Ajin Mandaokar, and John Browse -- Phenotyping jasmonate regulation of root growth / Fabian Kellermeier and Anna Amtmann -- Bioassays for assessing jasmonate-dependent defenses triggered by pathogens, herbivorous insects, or beneficial Rhizobacteria / Saskia C.M. Van Wees ... [et al.] -- Elicitation of jasmonate-mediated defense responses by mechanical wounding and insect herbivory / Marco Herde, Abraham J.K. Koo, and Gregg A. Howe -- Pseudomonas syringae infection assays in Arabidopsis / Jian Yao, John Withers, and Sheng Yang He -- Jasmonate signaling in the field, part I : elicited changes in jasmonate pools of transgenic Nicotiana attenuata / Emmanuel Gaquerel ... [et al.] -- Jasmonate Signaling in the field, part II : insect-guided characterization of genetic variations in jasmonate-dependent defenses of transgenic and natural Nicotiana attenuata populations / Emmanuel Gaquerel ... [et al.] -- Profiling of jasmonic acid-related metabolites and hormones in wounded leaves / Yusuke Jikumaru -- Non-targeted approach for extended liquid chromatography-mass spectrometry profiling of free and esterified jasmonates after wounding / Gaëtan Glauser and Jean-Luc Wolfender -- Cell-specific detection of jasmonates by means of an immunocytological approach / Bettina Hause, Kati Mielke, and Susanne Forner -- Jasmonic acid-amino acid conjugation enzyme assays / Martha L. Rowe and Paul E. Staswick -- Pull-down analysis of interactions among jasmonic acid core signaling proteins / Sandra Fonseca and Roberto Solano -- Yeast two-hybrid analysis of jasmonate signaling proteins / Amparo Pérez Cuéllar ... [et al.] -- Modified bimolecular fluorescence complementation assay to study the inhibition of transcription complex formation by JAZ proteins / Tiancong Qi, Susheng Song, and Daoxin Xie -- Agroinfiltration of Nicotiana benthamiana leaves for co-localization of regulatory proteins involved in jasmonate signaling / Volkan Çevik and Kemal Kazan -- Electrophoretic mobility shift assay for the analysis of interactions of jasmonic acid-responsive transcription factors with DNA / Johan Memelink -- Transient expression assays in tobacco protoplasts / Robin Vanden Bossche ... [et al.] -- Functional analysis of jasmonic acid-responsive secondary metabolite transporters / Nobukazu Shitan, Akifumi Sugiyama, and Kazufumi Yazaki -- Expression analysis of jasmonate-responsive lectins in plants / Nausicaä Lannoo and Els J.M. Van Damme -- Profiling the jasmonic acid responses by nuclear magnetic resonance-based metabolomics / Hye Kyong Kim, Young Hae Choi, and Robert Verpoorte -- Metabolite profiling of plant tissues by liquid chromatography fourier transform ion cyclotron resonance mass spectrometry / Jacob Pollier and Alain Goossens -- cDNA-AFLP-based transcript profiling for genome-wide expression analysis of jasmonate-treated plants and plant cultures / Janine Colling ... [et al.] -- Analysis of RNA-seq data with TopHat and cufflinks for genome-wide expression analysis of jasmonate-treated plants and plant cultures / Jacob Pollier, Stephane Rombauts, and Alain Goossens -- Transcriptome coexpression analysis using ATTED-II for integrated transcriptomic/metabolomic analysis / Keiko Yonekura-Sakakibara and Kazuki Saito -- Guide to CORNET for the construction of coexpression and protein-protein interaction networks / Stefanie De Bodt and Dirk Inzé.
  • 2011 Springer
    edited by Antonio Rodriguez-Moreno, Talvinder S. Sihra.
    "This volume critically examines the functional actions of the kainate type glutamate receptors (KARs). Following on from the larger body of work on the NMDA and AMPA-type ionotropic glutamate receptors (GluRs), studies with KARs have consistently thrown up exceptions to general rules about synaptic modulation. Contributors herein provide an insight to the idiosyncracies that now almost typify the KAR field. The fascinating insights provided in this volume serve to encourage searching mechanistic questions"--Provided by publisher.
    Also available: Print – 2011
  • 2008 Springer
    edited by Nenad Blau, Marinus Duran and K. Michael Gibson.
  • 2013 ScienceDirect
    edited by Jon Lorsch.
    Section I. Cell protocols : basic microbiological techniques. Pouring agar plates and streaking or spreading to isolate individual colonies / Elizabeth Eyler ; Storage of bacteria and yeast / Cheryl M. Koh -- Section II. Cell protocols : cellular fractionation. Cellular fractionation : mammalian cells / Joerg von Hagen and Uwe Michelsen ; Cellular fractionation : yeast cells / Joerg von Hagen and Uwe Michelsen -- Section III. Cell protocols : Knock-outs and gene replacements. Yeast-gene replacement using PCR products / Megan Bergkessel, Christine Guthrie, and John Abelson ; Measuring RNAi knockdown using qPCR / Stuart Milstein, Michael Nguyen, Rachel Meyers, and Antonin de Fougerolles ; Recombineering : using drug cassettes to knock out genes in vivo / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court ; Gene knockouts, in vivo site-directed mutagenesis and other modifications using the delitto perfetto system in Saccharomyces cerevisiae / Samantha Stuckey and Francesca Storici ; Gene targeting and site-specific recombination in mouse ES cells / Konstantinos Anastassiadis, Frank Schnütgen, Harald von Melchner, and A. Francis Stewart ; Recombineering : highly efficient in vivo genetic engineering using single-strand oligos / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court -- Section IV. Cell protocols : microbiological media. Growth media for E. coli / Juliane C. Lessard ; Saccharomyces cerevisiae growth media / Jessica S. Dymond --Section V. Cell protocols : preparation of slides for microscopy. Immunohistochemistry on freely floating fixed tissue sections / Julia Bachman ; Preparation of slides for microscopy from frozen tissue sections / Julia Bachman ; Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry / Kirstie Canene-Adams ; Preparation of cells for microscopy using cytospin / Cheryl M. Koh ; Preparation of cells for microscopy using chamber slides and coverslips / Cheryl M. Koh ; Preparation of cells for microscopy using 'cell blocks' / Cheryl M. Koh -- Section VI. Lipid and carbohydrate protocols. Enzymatic deglycosylation of glycoproteins / Min-Sung Kim and Dan Leahy -- Section VII. Lipid and carbohydrate protocol : making vesicles and micelles. Preparation of fatty acid or phospholipid vesicles by thin-film rehydration / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Preparation of fatty acid micelles / Ting F. Zhu, Itay Budin, and Jack W. Szostak -- Section VIII. Other protocols. Reverse-phase HPLC analysis and purification of small molecules / Kirstie Canene-Adams ; Thin layer chromatography / Marina Santiago and Scott Strobel ; ATP and GTP hydrolysis assays (TLC) / Vaishnavi Rajagopal and Jon R. Lorsch.
  • 2013 ScienceDirect
    edited by Jon Lorsch.
    Explanatory chapter: PCR primer design / Rubén Álvarez-Fernández -- How plasmid preparation kits work / Laura Koontz -- Introducing exogenous DNA into cells / Laura Koontz -- Agarose gel electrophoresis / Laura Koontz -- Analysis of DNA by southern blotting / Gary Glenn and Lefkothea-Vasiliki Andreou -- Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) / Sara Lopez-Gomollon and Francisco Esteban Nicolas -- Molecular cloning / Juliane C. Lessard -- Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® recombination cloning technology and Flp-In T-REx® lines / Jessica Spitzer, Markus Landthaler, and Thomas Tuschl -- Restrictionless cloning / Mikkel A. Algire -- Isolation of plasmid DNA from bacteria / Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from bacteria / Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from Saccharomyces cerevisiae / Jessica S. Dymond -- Isolation of genomic DNA from mammalian cells / Cheryl M. Koh -- Sanger dideoxy sequencing of DNA / Sarah E. Walker and Jon Lorsch -- Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform / Srinivasan Yegnasubramanian -- Next generation sequencing / Srinivasan Yegnasubramanian -- Generating mammalian stable cell lines by electroporation / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Transient mammalian cell transfection with polyethylenimine (PEI) / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Site-directed mutagenesis / Julia Bachman -- PCR-based random mutagenesis / Jessica S. Dymond -- Megaprimer method for mutagenesis of DNA / Craig W. Vander Kooi -- Troubleshooting PCR / Kirstie Canene-Adams -- Quantitative PCR / Jessica S. Dymond -- General PCR / Kirstie Canene-Adams -- Colony PCR / Megan Bergkessel and Christine Guthrie -- Chemical transformation of yeast / Megan Bergkessel and Christine Guthrie -- Transformation of E. coli via electroporation / Juliane C. Lessard -- Transformation of chemically competent E. coli / Rachel Green and Elizabeth J. Rogers.
  • 2013 ScienceDirect
    edited by Jon Lorsch.
    Computational prediction of RNA secondary structure -- Reverse-transcription PCR (RT-PCR) -- Northern blotting -- Explanatory chapter: nuclease protection assays -- In vitro transcription from plasmid or PCR-amplified DNA -- Measurement of in vivo synthesis rates -- Method for measuring mRNA decay rate in saccharomyces cerevisiae -- Analysis of polysomes from bacteria -- Polysome profile analysis - yeast -- Polysome analysis of mammalian cells -- Polysome analysis for determining mRNA and ribosome association in saccharomyces cerevisiae -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates -- Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3' ends, and poly(A) tails of RNA -- RNA radiolabeling -- Fluorescently labeling synthetic RNAs -- Analysis of RNA by analytical polyacrylamide gel -- RNA purification by preparative polyacrylamide gel -- Explanatory chapter: nucleic acid concentration determination -- RNA purification-precipitation methods -- Reverse transcriptase dideoxy sequencing of RNA -- RNA structure experimental analysis-chemical modification -- Structural analysis of RNA backbone using in-line probing.
  • 2013 Springer
    Vijai Kumar Gupta, Maria G. Tuohy, editors ; Manimaran Ayyachamy, Kevin M. Turner, Anthonia O'Donovan, associate editors.
    Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals.
  • 2011 Springer Protocols
    edited by Graeme I. Murray.
    Laser capture microdissection : methods and applications / Kristen DeCarlo . [and others] -- Laser microdissection for gene expression profiling / Lori A. Field [and others] -- Gene expression using the PALM system / Jian-Xin Lu and Cheuk-Chun Szeto -- Immunoguided microdissection techniques / Michael A. Tangrea [and others] -- Optimized RNA extraction from non-deparaffinized laser-microdissected material / Danny Jonigk [and others] -- Laser capture microdissection for analysis of gene expression in formalin-fixed paraffin-embedded Tissue / Ru Jiang, Rona S. Scott, and Lindsey M. Hutt-Fletcher -- MicroRNA profiling using RNA from microdissected immunostained tissue / Clemens L. Bockmeyer [and others] -- Profiling solid tumor heterogeneity by LCM and biological MS of fresh-frozen tissue sections / Donald J. Johann [and others] -- Amplification testing in breast cancer by multiplex ligation-dependent probe amplification of microdissected tissue / Cathy B. Moelans, Roel A. de Weger, and Paul J. van Diest -- Detection and quantification of MicroRNAs in laser-microdissected formalin-fixed paraffin-embedded breast cancer tissues / Sarkawt M. Khoshnaw [and others] -- Laser capture microdissection applications in breast cancer proteomics / Rene B.H. Braakman [and others] -- Proteomic analysis of laser microdissected ovarian cancer tissue with SELDI-TOF MS / Isabelle Cadron [and others] -- LCM Assisted biomarker discovery from archival neoplastic gastrointestinal tissues / Patricia A. Meitner and Murray B. Resnick -- Purification of diseased cells from Barrett's esophagus and related lesions by laser capture microdissection / Masood A. Shammas and Manjula Y. Rao -- Laser microdissection of intestinal epithelial cells and downstream analysis / Benjamin Funke -- Application of laser microdissection and quantitative PCR to assess the response of esophageal cancer to neoadjuvant chemo-radiotherapy / Claus Hann von Weyhern and Bjorn L.D.M. Brucher -- Oligonucleotide microarray expression profiling of contrasting invasive phenotypes in colorectal cancer / Christopher C. Thorn, Deborah Williams, and Thomas C. Freeman -- Evaluation of gastrointestinal mtDNA depletion in mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) / Carla Giordano and Giulia d'Amati -- Laser microdissection for gene expression study of hepatocellular carcinomas arising in cirrhotic and non-cirrhotic livers / Maria Tretiakova and John Hart -- Laser capture microdissection of pancreatic ductal adeno-carcinoma cells to analyze EzH2 by western blot analysis / Aamer M. Qazi [and others] -- Laser-capture microdissection of renal tubule cells and linear amplification of RNA for microarray profiling and real-time PCR / Susie-Jane Noppert, Susanne Eder, and Michael Rudnicki -- Subcellular renal proximal tubular mitochondrial toxicity with tenofovir treatment / James J. Kohler and Seyed H. Hosseini -- Application of laser-capture microdissection to study renal carcinogenesis / Kerstin Stemmer and Daniel R. Dietrich -- Laser-capture microdissection and transcriptional profiling in archival FFPE tissue in prostate cancer / Ajay Joseph and Vincent J. Gnanapragasam -- Quantitative analysis of the enzymes associated with 5-Fluorouracil metabolism in prostate cancer biopsies / Tomoaki Tanaka -- Microdissection of gonadal tissues for gene expression analyses / Anne Jørgensen, Marlene Danner Dalgaard, and Si Brask Sonne -- Duplex real-time PCR assay for quantifying mitochondrial DNA deletions in laser microdissected single spiral ganglion cells / Adam Markaryan, Erik G. Nelson, and Raul Hinojosa -- Neuronal type-specific gene expression profiling and laser-capture microdissection / Charmaine Y. Pietersen . [et al.] -- Region-specific in situ hybridization-guided laser-capture microdissection on postmortem human brain tissue coupled with gene expression quantification / Rene Bernard, Sharon Burke, and Ilan A. Kerman -- UV-laser microdissection and mRNA expression analysis of individual neurons from postmortem Parkinson's disease brains / Jan Grundemann, Falk Schlaudraff, and Birgit Liss -- Transcriptome profiling of murine spinal neurulation using laser capture microdissection and high-density oligonucleotide microarrays / Shoufeng Cao, Boon-Huat Bay, and George W. Yip -- Probing the CNS microvascular endothelium by immune-guided laser-capture microdissection coupled to quantitative RT-PCR / Nivetha Murugesan [and others] -- Laser-capture microdissection for factor VIII-expressing endothelial cells in cancer tissues / Tomoatsu Kaneko [and others] -- Laser-capture microdissection and analysis of liver endothelial cells from patients with Budd-Chiari syndrome / Selcuk Sozer and Ronald Hoffman -- Laser-capture microdissection of hyperlipidemic/ApoE-/- mouse aorta atherosclerosis / Michael Beer [and others] -- Gene expression profiling in laser-microdissected bone marrow megakaryocytes / Kais Hussein -- Specific RNA collection from the rat endolymphatic sac by laser-capture microdissection (LCM) : LCM of a very small organ surrounded by bony tissues / Kosuke Akiyama [and others] -- Use of laser capture microdissection on adult human articular cartilage for gene expression analysis / Naoshi Fukui, Yasuko Ikeda, and Nobuho Tanaka -- Laser-capture microdissection of developing barley seeds and cDNA array analysis of selected tissues / Johannes Thiel, Diana Weier, and Winfriede Weschke -- Quantitative RT-PCR gene expression analysis of a laser microdissected placenta : an approach to study preeclampsia / Yuditiya Purwosunu [and others].
  • 2013 Springer
    Uri Gophna, editor.
    "Although the phenomenon of lateral gene transfer has been known since the 1940s, it was the genomics era that has really revealed the extent and many facets of this evolutionary/genetic phenomenon. Even in the early 2000s with but a handful of genomes available, it became clear that the nature of microorganisms is full of genetic exchange between lineages that are sometimes far apart. The years following this saw an explosion of genomic data, which shook the "tree of life" and also raised doubts about the most appropriate species concepts for prokaryotes. This book represents the manyfold contributions of LGT to the evolution of micro and, to an extent, macroorganisms by focusing on the areas where it has the largest impact: metabolic innovations and adaptations and speciation." Back cover.
  • 2007
    John R. Bradley, David R. Johnson, Barbara R. Pober.
    Status: Not Checked OutLane Catalog Record
  • 2013 Springer Protocols
    edited by Ray J. Rose.
    Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.
  • 2007 Springer Protocols
    edited by Thomas J. McIntosh.
    Overview of membrane rafts / Thomas J. McIntosh -- Analysis of raft affinity of membrane proteins by detergent-insolubility / Deborah A. Brown -- Nondetergent isolation of rafts / Mehul B. Shah and Pravin B. Sehgal -- Detecting ordered domain formation (lipid rafts) in model membranes using tempo / Omar Bakht and Erwin London -- Using monomolecular films to characterize lipid lateral interactions / Rhoderick E. Brown and Howard L. Brockman -- Electro-formation and fluorescence microscopy of giant vesicles with coexisting liquid phases / Sarah L. Veatch -- Fluorescence correlation spectroscopy / Kristen Bacia and Petra Schwille -- Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells / Avery L. McIntosh ... [et al.] -- NMR detection of lipid domains / Ivan V. Polozov and Klaus Gawrisch -- Lateral diffusion coefficients of raft lipids from pulsed field gradient NMR / Greger Orädd and Göran Lindblom -- Saturation-recovery electron paramagnetic resonance discrimination by oxygen transport (DOT) method for characterizing membrane domains / Witold K. Subczynski ... [et al.] -- Plasmon-waveguide resonance spectoscopy studies of lateral segregation in solid-supported proteolipid bilayers / Zdzislaw Salamon, Savitha Devanathan, and Gordon Tollin -- Fluorescence recovery after photobleaching studies of lipid rafts / Anne K. Kenworthy -- Single-molecule tracking / Marija Vrljic, Stefanie Y. Nishimura, and W.E. Moerner -- X-ray diffraction to determine the thickness of raft and nonraft bilayers / Thomas J. McIntosh -- Small-angle neutron scattering to detect rafts and lipid domains / Jeremy Pencer ... [et al.] -- Exploring membrane domains using native membrane sheets and transmission electron microscopy / Bridget S. Wilson ... [et al.] -- Atomic force microscopy of lipid domains in supported model membranes / Alan R. Burns -- Atomistic and coarse-grained computer simulations of raft-like lipid mixtures / Sagar A. Pandit and H. Larry Scott -- A microscopic model calculation of the phase diagram of ternary mixtures of cholesterol and saturated and unsaturated phospholipids / R. Elliott, I. Szleifer, and M. Schick.
  • 2002 Springer Protocols
    edited by Subhash C. Basu, Manju Basu.
  • 2013 Springer Protocols
    edited by Helena Bäckvall and Janne Lehtiö.
    Part I. Low Molecular Weight Analysis Methods. Narrow-Range Peptide Isoelectric Focusing as Peptide Prefractionation Method Prior to Tandem Mass Spectrometry Analysis / Maria Pernemalm -- Analysis of Peptides by Denaturing Ultrafiltrationand LC-MALDI-TOF-MS / Yanming An and Radoslav Goldman -- Stable Isotope Labeling Methods in Protein Profiling / Johan Lengqvist and AnnSofi Sandberg -- Development of MRM-Based Assays for the Absolute Quantitation of Plasma Proteins / Michael A. Kuzyk, Carol E. Parker, Dominik Domanski, and Christoph H. Borchers -- Mass Spectrometric Profiling of Low-Molecular-Weight Proteins / Matthias Rainer, Constantin Sajdik, and Günther K. Bonn -- Developing an iMALDI Method / Brinda Shah, Jennifer D. Reid, Michael A. Kuzyk, Carol E. Parker, and Christoph H. Borchers -- Analysis of Neuropeptides by MALDI Imaging Mass Spectrometry / Anna Ljungdahl, Jörg Hanrieder, Jonas Bergquist, and Malin Andersson -- Highly Multiplexed Antibody Suspension Bead Arrays for Plasma Protein Profiling / Kimi Drobin, Peter Nilsson, and Jochen M. Schwenk -- Part II. Bioinformatics Of Proteomics Data. Protein Quantification by Peptide Quality Control (PQPQ) of Shotgun Proteomics Data / Jenny Forshed -- Part III Methods Applied In Clinical Research. Collection and Handling of Blood Specimens for Peptidomics / Harald Tammen and Rudiger Hess -- An Automated RP-SCX Solid-Phase Extraction Procedure for Urinary Peptidomics Biomarker Discovery Studies / Crina I.A. Balog, Rico Derks, Oleg A. Mayboroda, and André M. Deelder -- Application of Phage Display for Ligand Peptidomics to Identify Peptide Ligands Binding to AQP2-Expressing Membrane Fractions / Byung-Heon Lee and Tae-Hwan Kwon -- Protein Expression Profiling of Brain Tumor Tissue Using SELDI-MS / Carl Wibom -- Comprehensive Analysis of MHC Ligands in Clinical Material by Immunoaffinity-Mass Spectrometry / Kie Kasuga.
  • 2002 CRCnetBASE
    edited by Knox Van Dyke, Christopher Van Dyke, Karen Woodfork.
  • 2003 Springer Protocols
    v. 2 Springer Protocols
    edited by Barbara Driscoll.
    v. 1. Molecular pathology methods and reviews: Characteristic genetic alterations in lung cancer / Wistuba, II and A.F. Gazdar -- Clinical and biological relevance of recently defined categories of pulmonary neoplasia / E. Gabrielson -- Molecular epidemiology of human cancer risk. Gene-environment interactions and p53 mutation spectrum in human lung cancer / K. Vahakangas -- Neuroendocrine phenotype of small cell lung cancer / J.M. Coulson, M. Ocejo-Garcia and P.J. Woll -- Aids-associated pulmonary cancers / K. Katariya and R.J. Thurer-Abrogation of the rb-p16 tumor suppressor pathway in human lung cancer / J. Geradts -- Sensitive detection of hypermethylated p16ink4a alleles in exfoliative tissue material / M. Schuermann and M. Kersting -- Role of receptor tyrosine kinases in lung cancer / G. Maulik, T. Kijima and R. Salgia -- Relationship of egfr signal-transduction modulation by tyrosine kinase inhibitors to chemosensitivity and programmed cell death in lung cancer cell lines / W. Lei, P.P. Koty and M.L. Levitt -- Detection of the transcripts and proteins for the transforming growth factor-beta isoforms and receptors in mouse lung tumorigenesis using in situ hybridization and immunohistochemistry in paraffin-embedded tissue sections / S.B. Jakowlew and J. Mariano -- Alterations in the expression of insulin-like growth factors and their binding proteins in lung cancer / C.M. Coleman and A. Grimberg -- Screening of mutations in the ras family of oncogenes by polymerase chain reaction-based ligase chain reaction / A. Martinez [and others] -- Assays for raf-1 kinase phosphorylation and activity in human small cell lung cancer cells / R.K. Ravi and B.D. Nelkin -- Gamma-glutamylcysteine synthetase in lung cancer. Effect on cell viability / K. Jarvinen, Y. Soini and V.L. Kinnula -- Localization of cyclooxygenase-2 protein expression in lung cancer specimens by immunohistochemical analysis / S.A. Wardlaw and T.H. March -- Non-ri protocols for l-myc allelotyping and deletion mapping of chromosome 1p in primary lung cancers / K. Hiyama, C. Mendoza and E. Hiyama -- Intercellular adhesion molecules (icam-1, vcam-1, and lfa-1) in adenocarcinoma of lung. Technical aspects / A.E. Fraire [and others] -- Muc1 expression in lung cancer / E. Szabo -- Altered surface markers in lung cancer. Lack of cell-surface fas/apo-1 expression in pulmonary adenocarcinoma may allow escape from immune surveillance / Y. Nambu and D.G. Beer -- Prognostic relevance of angiogenic, proliferative, and apoptotic factors in lung carcinomas. A case review / M. Volm and R. Koomagi -- Morphological assessment of apoptosis in human lung cells / L.D. Dwyer-Nield [and others] -- [d-arg6, d-trp7,9, nmephe8]-substance p (6-11) activates jnk and induces apoptosis in small cell lung cancer cells via an oxidant-dependent mechanism / A. MacKinnon and T. Sethi -- Isolation of cells entering different programmed cell death pathways using a discontinuous percoll gradient / P.P. Koty [and others] -- Angiogenesis, metastasis, and lung cancer. An overview / A. Onn and R.S. Herbst -- Matrix metalloproteinase expression in lung cancer / M. Lim and D.M. Jablons -- Vascular endothelial growth factor expression in non-small cell lung cancer / K.J. O'Byrne [and others] -- Regulation of angiostatin mobilization by tumor-derived matrix metalloproteinase-2 / M.A. Moses and M.S. O'Reilly -- In vitro and in vivo assays for the proliferative and vascular permeabilization activities of vascular endothelial growth factor (vegf) and its receptor / S. Yano, R.S. Herbst and S. Sone -- Detection of telomerase activity in lung cancer tissues / K. Hiyama and E. Hiyama -- Analysis of alterations in a base-excision repair gene in lung cancer / N. Bhattacharyya and S. Banerjee -- Induction of lung cancer by passive smoking in an animal model system / H. Witschi -- Metastatic orthotopic mouse models of lung cancer / R.M. Hoffman -- Lung-specific expression of mutant p53 as mouse model for lung cancer / K.M. Tchou-Wong and W.N. Rom -- Use of nucleotide excision repair-deficient mice as a model for chemically induced lung cancer / D.L. Cheo and E.C. Friedberg -- Nude mouse lung metastases models of osteosarcoma and Ewing's sarcoma for evaluating new therapeutic strategies / S.F. Jia, R.R. Zhou and E.S. Kleinerman -- Tumor-specific metastasis to lung using reporter gene-tagged tumor cells / L.A. Culp [and others] -- Cultures of surgical material from lung cancers. A kinetic approach / B.C. Baguley, E.S. Marshall and T.I. Christmas -- The hollow fiber assay / L.A. Hall [and others]. v. 2. Diagnostic and therapeutic methods and reviews: Molecular and genetic aspects of lung cancer / W.N. Rom and K.M. Tchou-Wong -- Molecular alterations in lung cancer. Impact on prognosis / T. Kijima, G. Maulik and R. Salgia -- Cellular predictive factors of drug resistance in non-small cell lung carcinomas / M. Volm, R. Koomagi and W. Rittgen -- Clinical implications of p53 mutations in lung cancer / B.G. Campling and W.S. el-Deiry -- An epidemiologic and clinicopathologic overview of aids-associated pulmonary Kaposi's sarcoma / D.M. Aboulafia -- Myeloperoxidase promoter region polymorphism and lung cancer risk / X. Wu, M.B. Schabath and M.R. Spitz -- Clinical utility of tumor markers in the management of non-small cell lung cancer / D.E. Niewoehner and J.B. Rubins -- Detection of chromosomal aberrations in lung tissue and peripheral blood lymphocytes using interphase fluorescence in situ hybridization (fish) / R.A. el-Zein and S.Z. Abdel-Rahman -- In situ analysis of telomerase rna gene expression as a marker for tumor progression / W.N. Keith -- A high-throughput methodology for identifying molecular targets overexpressed in lung cancers / T. Wang and S.G. Reed -- Expression profiling of lung cancer based on suppression subtraction hybridization (ssh) / S. Petersen and I. Petersen -- Comparative genomic hybridization of human lung cancer / I. Petersen -- Sensitive assays for detection of lung cancer. Molecular markers in blood samples / M.V. Fournier [and others] -- Fluorescent microsatellite analysis in bronchial lavage as a potential diagnostic tool for lung cancer / J.K. Field and T. Liloglou -- Southern blotting of genomic DNA from lung and its tumors. Application to analysis of allele loss on chromosome 11p15.5 / D.M. Pitterle and G. Bepler -- Assessment of insulin-like growth factors and mutagen sensitivity as predictors of lung cancer risk / X. Wu [and others] -- Comparative multiplex pcr and allele-specific expression analysis in human lung cancer. Tools to facilitate target identification / J. Heighway [and others] -- Detection of k-ras point mutations in sputum from patients with adenocarcinoma of the lung by point-exacct / V.A. Somers and F.B. Thunnissen -- Detection of k-ras and p53 mutations by "mutant-enriched" pcr-rflp / M. Schuermann -- Detection of small cell lung cancer by rt-pcr for neuropeptides, neuropeptide receptors, or a splice variant of the neuron restrictive silencer factor / J.M. Coulson [and others] -- Utilization of thyroid transcription factor-1 immunostaining in the diagnosis of lung tumors / N.G. Ordonez -- Molecular biologic substaging of stage i nsclc through immunohistochemistry performed on formalin-fixed, paraffin-embedded tissue / M.B. Joshi, T.A. D'Amico and D.H. Harpole, Jr. -- A sensitive immunofluorescence assay for detection of p53 protein in the sputum / Z. Feng, D. Tian and R. Goldman -- Spr1. An early molecular marker for bronchial carcinogenesis / D. Lau [and others] -- Determination of biological parameters on fine-needle aspirates from non-small cell lung cancer / C. Bozzetti [and others] -- Detection and analysis of lung cancer cells from body fluids using a rare event imaging system / S.K. Kraeft, R. Salgia and L.B. Chen -- Reconstruction of geno-phenotypic evolutionary sequences from intracellular patterns of molecular abnormalities in human solid tumors / S.E. Shackney [and others] -- Surgical treatment of lung cancer. Past and present / C.F. Mountain and K.E. Hermes -- Recent advances and dilemmas in the radiotherapeutic management of locally advanced non-small cell lung cancer / B. Movsas -- Photodynamic therapy in lung cancer. A review / D. Ost -- Gene therapy for lung cancer. An introduction / E.B. Haura, E. Sotomayor and S.J. Antonia -- Identifying obstacles to viral gene therapy for lung cancer. Malignant pleural effusions as a paradigm / R.K. Batra, R.M. Bernal and S. Sharma -- Topical gene therapy for pulmonary diseases with pei-DNA aerosol complexes / A. Gautam [and others] -- Targeted delivery of expression plasmids to the lung via macroaggregated polyethylenimine-albumin conjugates / F.M. Orson [and others] -- Preparation of retroviral vectors for cell-cycle-targeted gene therapy of lung cancer / P.P. Claudio and A. Giordano -- Aerosol gene therapy for metastatic lung cancer using pei-p53 complexes / A. Gautam [and others] -- Clinical development of antisense oligonucleotides as anti-cancer therapeutics / H.X. Chen -- Antisense oligonucleotides targeting ri alpha subunit of protein kinase a. In vitro and in vivo anti-tumor activity and pharmacokinetics / R. Zhang and H. Wang -- Use of antisense oligonucleotides for therapy. Manipulation of apoptosis inhibitors for destruction of lung cancer cells / S.H. Leech, R.A. Olie and U. Zangemeister-Wittke -- Induction of programmed cell death with an antisense bcl-2 oligonucleotide / P.P. Koty, W. Lei and M.L. Levitt -- Tumor vaccination with cytokine-encapsulated microspheres / N.K. Egilmez [and others] -- Adoptive immunotherapy / J.A. Kim and S. Shu -- Intratumoral therapy with cytokine gene-modified dendritic cells in murine lung cancer models / S. Sharma [and others] -- Cyclooxygenase 2-dependent regulation of antitumor immunity in lung cancer / S. Sharma [and others] -- Chemoprevention of lung cancer / H. Witschi-Assessing the interaction of particulate delivery systems with lung surfactant / T.S. Wiedmann -- Chemopreventive therapeutics. Inhalation therapies for lung cancer and bronchial premalignancy / M. Haigentz, Jr. and R. Perez-Soler.
  • 2009 Springer
    David S. Goodsell.
    Introduction -- Molecular machines -- The processes of living -- Molecules in cells: Escherichia coli -- A human cell: The advantages of compartments -- The human body: The advantages of specialization -- Life and death -- Viruses -- You and your molecules.
  • 2012 Springer
    edited by Maria Armenia Carrondo and Paola Spadon.
  • v. 1-2, 2006. Springer Protocols
    v. 2, 2006 Springer Protocols
    edited by Sylvie Doublié.
    v. 1. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag / Joseph E. Tropea ... [et al.] -- Cloning, Production, and Purification of Proteins for a Medium-Scale Structural Genomics Project / Sophie Quevillon-Cheruel ... [et al.] -- Baculoviral Expression of an Integral Membrane Protein for Structural Studies / Dean R. Madden and Markus Safferling -- Protein Engineering / Sonia, Longhi, Francois Ferron, and Marie-Pierre Egloff -- Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems / Sylvie Doublié -- How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals / Gloria E. O. Borgstahl -- Screening and Optimization Methods for Nonautomated Crystallization Laboratories / Terese Bergfors -- Improving Marginal Crystals / Charles W. Carter, Jr. and Madeleine Riès-Kautt -- Optimization Techniques for Automation and High Throughput / Naomi E. Chayen -- Three-Dimensional Crystallization of Membrane Proteins / James Féthière -- Crystallization of Protein-DNA Complexes / Thomas Hollis -- Preparation and Crystallization of RNA / Barbara L. Golden -- Crystallization of RNA-Protein Complexes / Eiji Obayashi ... [et al.] -- Index. v. 2. Cryocrystallography of Macromolecules: Practice and Optimization / Elspeth Garman and Robin L. Owen -- Determination of Reaction Intermediate Structures in Heme Proteins / Kelvin Chu -- Annealing Macromolecular Crystals / B. Lief Hanson and Gerard J. Bunick -- First Analysis of Macromolecular Crystals: Biochemistry and X-Ray Diffraction / David Jeruzalmi -- X-Ray Data Collection from Macromolecular Crystals / Elspeth Garman and Robert M. Sweet -- Characterizing a Crystal from an Initial Native Dataset / Michael R. Sawaya -- Molecular Replacement / Eric A. Toth -- Phase Determination using Halide Ions / Miroslawa Dauter and Zbigniew Dauter -- The Same But Different: Isomorphous Methods for Phasing and High-Throughput Ligand Screening / Mark A. Rould -- Substructure Determination in Multiwavelength Anomalous Diffraction, Single Anomalous Diffraction, and Single Isomorphous Replacement with Anomalous Scattering Data Using Shake-and-Bake / G. David Smith, Christopher T. Lemke, and P. Lynne Howell -- Substructure Determination in Isomorphous Replacement and Anomalous Diffraction Experiments / Ralf W. Grosse-Kunstleve and Thomas R. Schneider -- Automated Structure Solution with autoSHARP / Clemens Vonrhein, Eric Blanc, Pietro Roversi, and Gérard Bricogne -- Introduction to Macromolecular Refinement / Dale E. Tronrud -- Quality Control and Validation / Gerard J. Kleywegt -- Crystallographic Software: A Sustainable Resource for the Community / Stephen J. Everse and Sylvie Doublié -- Index. v. 1. Preparation and crystallization of macromolecules -- v. 2. Structure determination.
  • 2011 Springer Protocols
    edited by Gyula Hadlaczky.
    [Publisher-supplied data] The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry. Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system. Written in the highly successful Methods in Molecular Biology series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.
  • 2009 Springer
    Andre Kamkin, Ilya Lozinsky, editors ; foreword by Maik Gollasch.
    The volume dwells on the major issues of mechanical stress influencing the ion channels and intracellular signaling pathways. This book is a unique collection of reviews outlining current knowledge and future developments in this rapidly growing field. In our opinion the book presents not only the latest achievements in the field but also brings the problem closer to the experts in related medical and biological sciences as well as practicing doctors. Knowledge of the mechanisms which underlie these processes is necessary for understanding of the normal functioning of different living organs and tissues and allows to predict changes, which arise due to alterations of their environment, and possibly will allow to develop new methods of artificial intervention. We also hope that presenting the problem will attract more attention to it both from researchers and practitioners and will assist to efficiently introduce it into the practical medicine.
  • 2011 Springer
    Andre Kamkin, Irina Kiseleva, editors ; foreward by Holger Scholz.
  • v. 1-, 2008- Springer
  • 2008
    edited by Steven R. Goodman.
    Status: Not Checked OutLane Catalog Record
  • 2012
    [edited by] Walter F. Boron, MD, PhD, professor, David N. and Inez Myers/Antonio Scarpa Chairman, Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio, Emile L. Boulpaep, MD, professor, Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut.
    Status: Not Checked OutLane Catalog Record
    Medical Physiology, in its updated 2nd edition, firmly relates molecular and cellular biology to the study of human physiology and disease. Drs. Walter Boron and Emile Boulpaep and a team of leading physiologists present practical, accurate coverage emphasizing the clinical implications of the material. Each chapter explains the principles and organization of each body system, while more than 1400 high-quality, full-color line drawings and prominently featured clinical examples clarify every concept. This exceptionally detailed and comprehensive guide to physiology is ideal for a rich, straightforward, state-of-the-art understanding of this essential subject.
  • 2017 ClinicalKey
    [edited by] Walter F. Boron, Emile L. Boulpaep.
  • 2015 Springer
    E. Anibal Disalvo, editor.
    1. Membrane hydration: a hint to a new model for biomembranes -- 2. Use of x-ray and neutron scattering methods with volume measurements to determine lipid bilayer structure and number of water molecules/lipid -- 3. Water and lipid bilayers -- 4. Hydration forces between lipid bilayers: a theoretical overview and a look on methods exploring dehydration -- 5. Monitoring membrane hydration with 2-(dimethylamino)-6-acylnaphtalenes fluorescent probes -- 6. Long-range lipid-water interaction as observed by ATR-FTIR spectroscopy -- 7. Hydration and nanoconfined water: insights from computer simulations -- 8. Aquaphotomics: near infrared spectoscopy and water states in biological systems -- 9. Hydration in lipid monolayers: correlation of water activity and surface pressure -- 10. Water at biological phase boundaries: its role in interfacial activation of enzymes and metabolic pathways -- 11. Anhydrobiosis: an unsolved problem with applications in human welfare -- Concluding remarks -- Index.
  • 2003 Springer Protocols
    edited by Barry S. Selinsky.
    Expression and purification of the amphipathic form of rabbit cytochrome b5 in escherichia coli / L. Waskell -- Dihydroorotate dehydrogenase of escherichia coli / K.F. Jensen and S. Larsen -- A general approach for heterologous membrane protein expression in escherichia coli: The uncoupling protein, ucp1, as an example / A.Z. Shaw and B. Miroux -- Expression of membrane-bound iron-sulfur proteins / D. Schneider, C.L. Schmidt and A. Seidler -- Heterologous expression of human scavenger receptor class b type i (sr-bi) in pichia pastoris / C.H. Han [and others] -- Expression of oligomeric amiloride-sensitive epithelial sodium channel in sf9 insect cells / U.S. Rao -- Functional expression of his-tagged rhodopsin in sf9 insect cells / G.J. Bosman [and others] -- Preparation of glycerol facilitator for protein structure and folding studies in solution / D. Manley and J.D. O'Neil -- Solubilization of chemokine receptors from cell membranes / R. Staudinger and J.C. Bandres -- A systematic approach for the solubilization of the integral membrane protein lysophospholipid: Acyl-coa acyltransferase (lat) / C. Kerkhoff and V. Kaever -- Purification of omp50, a minor porin of campylobacter jejuni / J.M. Bolla -- Purification of porins from mycobacterium smegmatis / C. Heinz, E. Roth and M. Niederweis -- Isolation of the melanocortin 5 receptor: From cdna sequence to isolating an integral membrane protein / B.L. Clarke -- Purification of mammalian serine palmitoyltransferase, a hetero-subunit enzyme for sphingolipid biosynthesis, by affinity-peptide chromatography / K. Hanada and M. Nishijima -- Purification of phosphatidylglycerophosphate synthase from cultured mammalian cells / K. Kawasaki and M. Nishijima -- Purification of pancreastatin receptor from rat liver membranes / V. Sanchez-Margalet, J. Santos-Alvarez and S. Diaz-Troya -- Purification, reconstitution, and functional characterization of zinc transporter from rat renal brush border membranes / R. Prasad -- Isolation of lipid raft-associated proteolipids / J. Millan [and others] -- Purification of membrane-bound catechol-o-methyltransferase / M.J. Bonifacio and P. Soares-da-Silva -- Purification and characterization of transporter proteins from human erythrocyte membrane / D.N. Wang, M.J. Lemieux and J.M. Boulter -- Purification of the human erythrocyte ps-stimulated mg2+-atpase: A putative ps flippase / J.V. Lyles [and others] -- Reconstitution and assay of biogenic membrane-derived phospholipid flippase activity in proteoliposomes / S.N. Gummadi [and others] -- Expression, purification, and reconstitution of rat liver carnitine palmitoyltransferase i / N.F. Brown -- Crystallization in lipidic cubic phases: A case study with bacteriorhodopsin / V.I. Gordeliy [and others] -- Optical biosensor assay using retroviral receptor pseudotypes / J. Rucker.
  • 2009 Springer Protocols
    edited by Matthew J. Peirce and Robin Wait.
    Online tools for predicting integral membrane proteins / H. Bigelow and B. Rost -- In silico identification of novel G protein coupled receptors / M.N. Davies and D.R. Flower -- Transcriptome-based identification of candidate membrane proteins / E.J. Evans ... [et al.] -- Separation of thylakoid membrane proteins by sucrose gradient ultracentrifugation or blue native-SDS-PAGE two-dimensional electrophoresis / G.M. D'Amici, C.G. Huber and L. Zolla -- Extraction of yeast mitochondrial membrane proteins by solubilization and detergent/polymer aqueous two-phase partitioning / H. Everberg, N. Gustavsson and F. Tjerneld -- 16-BAC/SDS-PAGE analysis of membrane proteins of yeast mitochondria purified by free flow electrophoresis / R.J. Braun ... [et al.] -- Sequential detergent extraction prior to mass spectrometry analysis / F.M. McCarthy, A.M. Cooksey and S.C. Burgess -- Enrichment of brain plasma membranes by affinity two-phase partitioning / J. Schindler and H.G. Nothwang -- Protocol to enrich and analyze plasma membrane proteins / R.J. Wisniewski -- Proteomic analysis of the lymphocyte plasma membrane using cell surface biotinylation and solution-phase isoelectric focusing / M.J. Peirce, A.P. Cope and R. Wait -- Identification of target membrane proteins as detected by phage antibodies / C.A. Geuijen, A.Q. Bakker and J. de Kruif -- Membrane protease degradomics: proteomic identification and quantification of cell surface protease substrates / G.S. Butler ... [et al.] -- Purification of basolateral integral membrane proteins by cationic colloidal silica-based apical membrane subtraction / R.J. Goode and R.J. Simpson -- Moving closer to the lipid raft proteome using quantitative proteomics / L.J. Foster -- Use of sequential chemical extractions to purify nuclear membrane proteins for proteomics identification / N. Korfali ... [et al.] -- Isolation of extracellular membranous vesicles for proteomic analysis / R.A. Mathias ... [et al.] -- Enrichment of human platelet membranes for proteomic analysis / D.W. Greening ... [et al.] -- Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis / T. Rabilloud -- Two-dimensional separation of membrane proteins by 16-BAC-SDS-PAGE / H.G. Nothwang and J. Schindler -- MudPIT analysis: application to human heart tissue / K.G. Kline and C.C. Wu -- Liquid chromatography MALDI MS/MS for membrane proteome analysis / N. Wang, J.B. Young and L. Li -- Cysteinyl-tagging of integral membrane proteins for proteomic analysis using liquid chromatography-tandem mass spectrometry / S.K. Mitra and M.B. Goshe -- Quantitative proteomics analysis of pancreatic zymogen granule membrane proteins / X. Chen and P.C. Andrews.
  • 2003 Springer Protocols
    edited by Qing Yan.
    Pharmacogenomics of membrane transporters an overview / Q. Yan -- The iubmb-endorsed transporter classification system / W. Busch and M.H. Saier, Jr. -- Bioinformatics and data integration in membrane transporter studies / Q. Yan -- DNA microarrays to analyze gene expression in animals with altered transporter expression / G.W. Miller -- Microarray data analysis in studies of membrane transporters / D. Hu -- Laser capture microdissection to examine transporter expression in specific cell regions / O. Rho and G.W. Miller -- Site-directed mutagenesis in the study of membrane transporters / R.M. Ryan, A.D. Mitrovic and R.J. Vandenberg -- Fluorescence techniques for studying membrane transport proteins: The p-glycoprotein multidrug transporter / F.J. Sharom [and others] -- Reconstitution of membrane transporters / F.J. Sharom and P.D. Eckford -- Equilibrium binding and transport studies / A.M. Ojeda [and others] -- Electrophysiological studies of ion channels and therapeutic potential with adenovirus-mediated gene transfer / J. Qu, T.V. Pham and M.N. Obreztchikova -- Antibody production and immunocytochemical localization of amino acid transporters / D.V. Pow, R. Sullivan and H. Scott -- Xenopus laevis oocytes / S. Broer -- Measurement of intracellular ph / F.B. Loiselle and J.R. Casey -- Small-angle x-ray scattering studies of membrane transporter peripheral components and soluble domains / B.H. Shilton -- Membrane protein nmr studies / S.J. Opella -- Analysis of soluble sugar permease domains by solution nmr / T.A. Wilkinson and Y. Chen -- Molecular modeling and molecular dynamics simulations of membrane transporter proteins / D.S. Dwyer -- Imaging plasma membrane proteins by confocal microscopy / S.W. Tsang and P.M. Haney.
  • 2011 Springer
    Karen E. Nelson, editor ; foreword by Jane L. Peterson and Susan Garges.
    1.The human genome, microbiomes, and disease -- 2. Host genotype and the effect on microbial communities -- 3. The human microbiome and host-pathogen interactions -- 4. The human virome -- 5. Selection and sequencing of strains as references for human microbiome studies -- 6. The human vaginal microbiome -- 7. The human lung microbiome -- 8. The human skin microbiome in health and skin diseases -- 9. The human oral microbiome -- 10. Retraction: the human and his microbiome risk factors for infections -- 11. Retraction: infectogenomics: aspect of host responses to microbes in digestive tract -- 12. Autoimmune disease and human metagenome -- 13. Metagenomic applications and the potential for understanding chronic liver disease -- 14. Symbiotic gut microbiota and the modulation of human metabolic phenotypes -- 15. MetaHIT: the European Union project on metagenomics of the human intestinal tract -- 16. Implications of human microbiome research for the developing world -- Index.
  • 2014 Springer Protocols
    edited by Yinduo Ji.
    Clinical, epidemiologic, and laboratory aspects of methicillin-resistant Staphylococcus aureus infections / Elizabeth L. Palavecino -- Community-associated methicillin-resistant Staphylococcus aureus case studies / Madeleine G. Sowash and Anne-Catrin Uhlemann -- Rapid methods for detection of MRSA in clinical specimens / Elizabeth L. Palavecino -- Immunofluorescence microscopy for the detection of surface antigens in methicillin-resistant Staphylococcus aureus (MRSA) / Yekaterina Timofeyeva, Ingrid L. Scully, and Annaliesa S. Anderson -- Internal transcribed spacer (ITS)-PCR identification of MRSA / Shin-ichi Fujita -- Pulsed-field gel electrophoresis typing of Staphylococcus aureus isolates / Yiping He, Yanping Xie, and Sue Reed -- Multilocus sequence typing (MLST) of Staphylococcus aureus / Nicholas A. Saunders and Anne Holmes -- Staphylococcal cassette chromosome mec (SCCmec) analysis of MRSA / Teruyo Ito ... [and 6 others] -- Genetic interruption of target genes for investigation of virulence factors / Adhar C. Manna -- Molecular analysis of staphylococcal superantigens / Wilmara Salgado-Pabón, Laura C. Case-Cook, and Patrick M. Schlievert -- Investigation of Staphylococcus aureus adhesion and invasion of host cells / Junshu Yang and Yinduo Ji -- Investigation of biofilm formation in clinical isolates of Staphylococcus aureus / James E. Cassat, Mark S. Smeltzer, and Chia Y. Lee -- Transcriptomic analysis of Staphylococcus aureus using microarray and advanced next-generation RNA-seq technologies / Ting Lei, Aaron Becker, and Yinduo Ji -- Proteomic aproach to investigate pathogenicity and metabolism of methicillin-resistant Staphylococcus aureus / Patrice Franc̦ois ... [and 3 others] -- Metabolomic investigation of methicillin-resistant Staphylococcus aureus / Ting Lei ... [and 3 others] -- Treatment of infections due to resistant Staphylococcus aureus / Gregory M. Anstead, Jose Cadena, and Heta Javeri -- Anti-infective drug development for MRSA / Anu Daniel -- Animal models in drug development for MRSA / Andrea Marra.
  • 2007 Springer Protocols
    edited by Yinduo Ji.
    Clinical, epidemiologic, and laboratory aspects of methicillin-resistant Staphylococcus aureus (MRSA) infections / Elizabeth Palavecino -- MRSA case studies / Kurt D. Reed, Mary E. Stemper, and Sanjay K. Shukla -- MIC analysis and susceptibility testing of MRSA / German Bou -- ITS-PCR identification of MRSA / Shin-ichi Fujita -- Pulsed-field gel electrophoresis of methicillin-resistant Staphylococcus aureus / Kurt D. Reed, Mary E. Stemper, and Sanjay K. Shukla -- Multilocus sequence typing (MLST) of Staphylococcus aureus / Nicholas A. Saunders and Anne Holmes -- Staphylococcal cassette chromosome mec (SCCmec) analysis of MRSA / Teruyo Ito, Kyoko Kuwahara, and Keiichi Hiramatsu -- Targeted gene disruption for the analysis of virulence of Staphylococcus aureus / J. Ross Fitzgerald -- Molecular analysis of staphylococcal superantigens / Patrick M. Schlievert and Laura C. Case -- Investigation of biofilm formation in clinical isolates of Staphylococcus aureus / James E. Cassat, Chia Y. Lee, and Mark S. Smeltzer -- Comparative analysis of staphylococcal adhesion and internalization by epithelial cells / Xudong Liang and Yinduo Ji -- Comparative analysis of MRSA / Fumihiko Takeuchi, Tadashi Baba, and Keiichi Hiramatsu -- Genomic analysis of gene expression of Staphylococcus aureus / Chuanxin Yu ... [et al.] -- Proteomic approach to investigate methicillin-resistant Staphylococcus aureus / Patrice Francois ... [et al.] -- Environmental surveillance for methicillin-resistant Staphylococcus aureus / J. Scott Weese -- The control and prevention of MRSA infections / Liangsu Wang and John F. Barrett -- Treatment of infections due to resistant Staphylococcus aureus / Gregory M. Anstead, Gabriel Quinones-Nazario, and James S. Lewis II.
  • 2013 ScienceDirect
    edited by Franck Perez and David J. Stephens.
  • 2015 Springer Protocols
    edited by Dylan M. Owen.
    Introduction to membrane lipids /Richard M. Epand -- Introduction : membrane properties (good) for life /Marek Cebecauer -- Introduction to fluorescence probing of biological membranes /Alexander P. Demchenko ... [et al.] -- Assembly and use of tethered bilayer lipid membranes (tBLMs) /Charles Cranfield ... [et al.] -- Preparation of detergent-resistant membranes (DRMs) from cultured mammalian cells /Deborah A. Brown -- Isolation of giant plasma membrane vesicles for evaluation of plasma membrane structure and protein partitioning /K. R. Levental and I. Levental -- Asymmetric giant lipid vesicle fabrication /Peichi C. Hu and Noah Malmstadt -- Cholesterol depletion using Methyl-[Beta]-cyclodextrin /Saleemulla Mahammad and Ingela Parmryd -- Comparative LC-MS based profiling approach to analyze lipid composition in tissue culture systems /G. Ekin Atilla-Gokcumen and Ulrike S. Eggert -- Imaging membrane order using environmentally sensitive fluorophores /G.W. Ashdown and Dylan M. Owen -- 3D super-resolution imaging by localization microscopy /Astrid Magenau and Katharina Gaus -- Electron microscopy methods for studying plasma membranes /Alison J. Beckett and Ian A. Prior -- Measuring cytoskeleton and cellular membrane mechanical properties by atomic force microscopy /Charles Roduit ... [et al.] -- Fluorescence linear dichroism imaging for quantifying membrane order /Richard K. P. Benninger -- Scanning fluorescence correlation spectroscopy on biomembranes /Eduard Hermann, Jonas Ries, and Ana J. García-Sáez -- X-ray diffraction of lipid model membranes /Arwen I. I. Tyler, Robert V. Law, and John M. Seddon -- Solid state NMR of lipid model membranes /Arwen I. I. Tyler ... [et al.] -- Fluorescence recovery after photobleaching (FRAP) : acquisition, analysis, and applications /Michael Carnell, Alex Macmillan, and Renee Whan -- Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells /Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton -- Cholesterol behavior in asymmetric lipid bilayers : insights from molecular dynamics simulations /Semen O. Yesylevskyy and Alexander P. Demchenko -- Computer simulations of phase separation in lipid bilayers and monolayers /Svetlana Baoukina and D. Peter Tieleman.
  • 2007 Springer Protocols
    edited by Alex M. Dopico.
  • v. 1-, 1984- Springer Protocols
    Turksen, Kursad; Walker, John M.
  • v. 1-, 1996- Springer Protocols
    Walker, John M.
  • 2015 Springer Protocols
    edited by Vicente Andres, Beatriz Dorado.
    Use of mouse models in atherosclerosis research / Godfrey S. Getz and Catherine A. Reardon -- Cytokines and immune responses in murine atherosclerosis / Pascal J. H. Kusters and Esther Lutgens -- Intravital microscopy for atherosclerosis research / Remco T. A. Megens and Oliver Soehnlein -- Noninvasive molecular imaging of mouse atherosclerosis / Martina A. McAteer and Robin P. Choudhury -- Oil red O and hematoxylin and eosin staining for quantification of atherosclerosis burden in mouse aorta and aortic root / M. Jesús Andrés-Manzano, Vicente Andrés, and Beatriz Dorado -- Isolation, culture, and polarization of murine bone marrow-derived and peritoneal macrophages / Inés Pineda-Torra ... [et al.] -- Isolation of mouse primary aortic endothelial cells by selection with specific antibodies / Pedro Molina-Sánchez and Vicente Andrés -- Isolation and culture of aortic smooth muscle cells and in vitro calcification assay / Ricardo Villa-Bellosta and Magda R. Hamczyk -- Immunostaining of macrophages, endothelial cells, and smooth muscle cells in the atherosclerotic mouse aorta / Prashanthi Menon and Edward A. Fisher -- Immunostaining of lymphocytes in mouse atherosclerotic plaque / Anton Gisterå and Daniel F. J. Ketelhuth -- Flow cytometric analysis of immune cells within murine aorta / Breanne N. Gjurich, Parésa L. Taghavie-Moghadam, and Elena V. Galkina -- In vitro differentiation of naïve CD4+ T cells : a tool for understanding the development of atherosclerosis / Salvador Iborra and Jose M. González-Granado -- Quantification of apoptosis in mouse atherosclerotic lesions / Nichola L. Figg and Martin R. Bennett -- Quantification of cellular proliferation in mouse atherosclerotic lesions / José J. Fuster -- Quantification of in vitro macrophage cholesterol efflux and in vivo macrophage-specific reverse cholesterol transport / Joan Carles Escolà-Gil ... [et al.] -- In vitro macrophage phagocytosis assay / Magda R. Hamczyk, Ricardo Villa-Bellosta, and Vicente Andrés -- Glucose and insulin tolerance tests in the mouse / Ángela Vinué and Herminia González-Navarro -- Wire myography to study vascular tone and vascular structure of isolated mouse arteries / Lara del Campo and Mercedes Ferrer -- Pressure myography to study the function and structure of isolated small arteries / Olav L. Schjørring, Rune Carlsson, and Ulf Simonsen -- Generation of aorta transcript atlases of wild-type and apolipoprotein E-null mice by laser capture microdissection-rased mRNA expression microarrays / Changjun Yin ... [et al.] -- Analysis of gene and protein expression in atherosclerotic mouse aorta by western blot and quantitative real-time PCR / José Rivera-Torres -- Bone marrow transplantation in mice to study the role of hematopoietic cells in atherosclerosis / Vinatha Sreeramkumar and Andrés Hidalgo -- Tandem stenosis to induce atherosclerotic plaque instability in the mouse / Yung Chih Chen, Jennifer Rivera, and Karlheinz Peter -- Detection of intraplaque hemorrhage in mouse atherosclerotic lesions / Judith C. Sluimer, Marion J. Gijbels, and Sylvia Heeneman -- Mechanical stabilization of mouse carotid artery for in vivo intravital microscopy imaging of atherogenesis / Raphaël Chèvre -- Intravital microscopy in the cremaster muscle microcirculation for endothelial dysfunction studies / Cristina Rius and María J. Sanz -- Fluorescent molecular tomography for in vivo imaging of mouse atherosclerosis / Alicia Arranz ... [et al.] -- In vivo 18F-FDG-PET imaging in mouse atherosclerosis / Jesús Mateo ... [et al.] -- Magnetic resonance imaging of the atherosclerotic mouse aorta / Jesús Mateo ... [et al.].
  • 2011 ScienceDirect
    edited by Daniel Jameson, Malkhey Verma, and Hans V. Westerhoff.
    Also available: Print – 2011
  • v. 4-5, 2005-07. Springer
    v. 5 (2007) Springer
  • NCBI Bookshelf
    This searchable database is a collection of in vivo molecular imaging agents developed for positron emission tomography (PET), single photon emission computed tomography (SPECT), magnetic resonance imaging (MRI), ultrasound (US), computed tomography (CT), and optical imaging. MICAD, a key component of the "Molecular Libraries and Imaging" program of the NIH Roadmap, is being developed by NCBI, the National Center for Biotechnology Information".
  • 2009 Springer
    edited by Jonathan R. Pollack.
    DNA microarray technology. Introduction / J.R. Pollack -- Comparative genomic hybridization on BAC arrays / B.P. Coe ... [et al.] -- Comparative genomic hybridization on spotted oligonucleotide microarrays / Y.H. Kim and J.R. Pollack -- Comparative genomic hybridization by representational oligonucleotide microarray analysis / R. Lucito and J. Byrnes -- Application of oligonucleotides arrays for coincident comparative genomic hybridization, ploidy status and loss of heterozygosity studies in human cancers / J.K. Cowell and K.C. Lo -- Molecular inversion probe assay for allelic quantitation / H. Ji and K. Welch -- A whole-genome amplification protocol for a wide variety of DNAs, including those from formalin-fixed and paraffin-embedded tissue / P.L. Paris -- Algorithms for calling gains and losses in array CGH data / P. Wang -- Methylation analysis by microarray / D.E. Deatherage ... [et al.] -- Methylation analysis by DNA immunoprecipitation (MeDIP) / E.A. Vucic ... [et al.] -- Combining chromatin immunoprecipitation and oligonucleotide tiling arrays (ChIP-Chip) for functional genomic studies / J. Eeckhoute, M. Lupien and M. Brown -- ChIP-Chip: algorithms for calling binding sites / X.S. Liu and C.A. Meyer -- Mapping regulatory elements by DNaseI hypersensitivity chip (DNase-Chip) / Y. Shibata and G.E. Crawford -- Microarray analysis of DNA replication timing / N. Karnani, C.M. Taylor and A. Dutta -- Integration of diverse microarray data types / K. Salari and J.R. Pollack.
    Also available: Print – 2009
  • 2012 Springer
    Bertrand Jordan, editor.
    Microarray technology has made strong progress over the past decade, and there have also been significant changes in application areas, from nucleic acids to proteomics and from research to clinical applications. This book provides a comprehensive overview of microarrays in diagnostics and biomarker development, covering DNA, peptide, protein and tissue arrays. The focus is on entities that are in actual clinical use, or quite close, and on recent developments, such as peptide and aptamer arrays. A further topic is the miniaturisation towards nanoarrays, which is expected to have great potential in clinical applications. Relevant issues of bioinformatics and statistical analysis of array data are discussed in detail, as well as the barriers to the commercialisation of array-based tests and the vexing IP issues involved. Thus, the book should be very useful tor active array users as well as to newcomers seeking to make the best choice between different technologies.
  • 2011 Springer
    G.N. Cohen.
    Bacterial growth -- The outer membrane of gram-negative bacteria and the cytoplasmic membrane -- Peptidoglycan synthesis and cell division -- Cellular permeability -- Allosteric enzymes -- Glycolysis, gluconeogenesis and glycogen synthesis -- The pentose phosphate and Entner-Doudoroff pathways -- The tricarboxylic acid cycle and the glyoxylate bypass -- ATP-generating processes : respiration and fermentation -- Biosynthesis of lipids -- Iron-sulfur proteins -- The archaea -- Methanogens and methylotrophs -- Enzyme induction in catabolic systems -- Transcription : RNA polymerase -- Negative regulation -- Enzyme repression in anabolic pathways -- Positive regulation -- The ribosomes -- The genetic code, the transfer RNAs and the aminoacyl-tRNA-synthetases -- Attenuation -- Riboswitches -- The biological fixation of nitrogen -- How biosynthetic pathways have been established -- The aspartic acid family of amino acids : biosynthesis -- Regulation of the biosynthesis of the amino acids of the aspartic acid family in Enterobacteriaceae -- Other patterns of regulation of the synthesis of amino acids of the aspartate family -- Biosynthesis of the amino acids of the glutamic acid family and its regulation -- Biosynthesis of amino acids derived from phosphoglyceric acid and pyruvic acid -- Selenocysteine and selenoproteins -- Biosynthesis of aromatic amino acids and its regulation -- The biosynthesis of histidine and its regulation -- The biosynthesis of nucleotides -- The biosynthesis of deoxyribonucleotides -- Biosynthesis of some water-soluble vitamins and of their coenzyme forms -- Biosynthesis of carotene, vitamin A, sterols, ubiquinones and menaquinones -- Biosynthesis of the tetrapyrrole ring system -- Biosynthesis of cobalamins including vitamin B12 -- Interactions between proteins and DNA -- Evolution of biosynthetic pathways.
  • 2006 Wiley
    [edited by] Ian Humphery-Smith, Michael Hecker.
    "Discover important lessons learned about whole organism biology via microbial proteomics This text provides an exhaustive analysis and presentation of current research in the field of microbial proteomics, with an emphasis on new developments and applications and future directions in research. The editors and authors show how and why the relative simplicity of microbes has made them attractive targets for extensive experimental manipulation in a quest for both improved disease prevention and treatment and an improved understanding of whole organism functional biology. In particular, the text demonstrates how microbial proteomic analyses can aid in drug discovery, including identification of new targets, novel diagnostic markers, and lead optimization. Each chapter is written by one or more leading experts in the field and carefully edited to ensure a consistent and thorough approach throughout. Methods, technologies, and tools associated with the most promising approaches are stressed."--Publisher's website.
    Also available: Print – 2006
  • 2013 Springer Protocols
    edited by Gareth Jenkins, Colin D. Mansfield.
    Present technology and future trends in point-of-care microfluidic diagnostics / Lawrence Kulinsky, Zahra Noroozi, and Marc Madou -- Teaching microfluidic diagnostics using Jell-O® chips / Cheng Wei T. Yang and Eric T. Lagally -- Fundamentals of microfluidics for high school students with no prior knowledge of fluid mechanics / Vishal Tandon and Walter Peck -- Measuring microchannel electroosmotic mobility and zeta potential by the current monitoring method / Chenren Shao and Don L. DeVoe -- Overview of the microfluidic diagnostics commercial landscape / Lily Kim -- Practical aspects of the preparation and filing of patent applications / Fiona Bessoth -- Introduction to in vitro diagnostic device regulatory requirements / Jonathan Day -- Microfluidic device fabrication by thermoplastic hot-embossing / Shuang Yang and Don L. DeVoe -- Introduction to glass microstructuring techniques / Radoslaw Mazurczyk and Colin D. Mansfield -- Glass microstructure capping and bonding techniques / Radoslaw Mazurczyk, Colin D. Mansfield, and Marcin Lygan -- Rapid prototyping of PDMS devices using SU-8 lithography / Gareth Jenkins -- Microfluidic interface technology based on stereolithography for glass-based lab-on-a-chips / Song-I Han and Ki-Ho Han -- Three-dimensional, paper-based microfluidic devices containing internal timers for running time-based diagnostic assays / Scott T. Phillips and Nicole K. Thom -- Thread based devices for low-cost diagnostics / Meital Reches -- Droplet-based microfluidics / Sanjiv Sharma [and others] -- Droplet-based microfluidics for binding assays and kinetics based on FRET / Monpichar Srisa-Art and Sanjiv Sharma -- Surface treatments for microfluidic biocompatibility / N.J. Shirtcliffe, R. Toon, and P. Roach -- Superhydrophobicity for antifouling microfluidic surfaces / N.J. Shirtcliffe and P. Roach -- Application of microfluidic devices for viral diagnosis in developing countries / Samantha M. Hattersley, John Greenman, and Stephen J. Haswell -- Applications of microfluidics for molecular diagnostics / Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale -- Quantitative heterogeneous immunoassays in protein modified polydimethylsiloxane microfluidic channels for rapid detection of disease biomarkers / Peng Li -- Breast cancer diagnostics using microfluidic multiplexed immunohistochemistry / Minseok S. Kim, Seyong Kwon, and Je-Kyun Park -- Charged-coupled device (CCD) detectors for lab-on-a chip (LOC) optical analysis / Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck -- Multilayer microfluidic poly(ethylene glycol) diacrylate hydrogels / Michael P. Cuchiara and Jennifer L. West -- Purification of DNA/RNA in a microfluidic device / Andy Fan, Samantha Byrnes, and Catherine Klapperich -- Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR / Xuefei Leng and Chaoyong James Yang -- Integrated fluidic circuits (IFCs) for digital PCR / Ramesh Ramakrishnan [and others] -- microFIND® approach to fluorescent in situ hybridization (FISH) / Andrea Zanardi, Emanuele Barborini, and Roberta Carbone -- ELISA lab-on-a-chip (ELISA-LOC) / Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov -- Multiplexed surface plasmon resonance imaging for protein biomarker analysis / Eric Ouellet, Louise Lund, and Eric T. Lagally -- Surface acoustic wave (SAW) biosensors : coupling of sensing layers and measurement / Kerstin Lange, Friederike J. Gruhl, and Michael Rapp -- Microchip UV absorbance detection applied to isoelectric focusing of proteins / Junjie Ou and Carolyn L. Ren.
  • 2015 Springer
    Gaetano Santulli, editor.
    microRNAs in the malignant transformation process / Anne E. Sarver, Lihua Li, Reena V. Kartha, Subbaya Subramanian -- microRNA and chronic lymphocytic leukemia / Dana Elena Giza, George A. Calin -- microRNA in malignant lymphoma / Hiroyuki Tagawa -- MicroRNAs and gastrointestinal stromal tumor / Pinar Akçakaya, Weng-Onn Lui -- Insights into the role of microRNAs in pancreatic cancer pathogenesis: potential for diagnosis, prognosis, and therapy / Mohammad Aslam Khan [and others] -- microRNAs and colorectal cancer / Anna Lena Ress, Samantha Perakis, Martin Pichler -- microRNAs and prostate cancer / Sajni Josson, Leland W. K. Chung, Murali Gururajan -- microRNA and ovarian cancer / Ream Langhe -- microRNA and lung cancer / Valerio Del Vescovo, Michela A. Denti -- microRNAs and soft tissue sarcomas / Tomohiro Fujiwara, Toshiyuki Kunisada, Ken Takeda, Toshifumi Ozaki -- microRNA and bone cancer / Mary Nugent.
    Also available: Print – 2015
  • 2015 Springer
    Gaetano Santulli, editor.
    Exploiting microRNA specificity and selectivity: paving a sustainable path towards precision medicine / Gaetano Santulli -- microRNAs and personalized medicine: evaluating their potential as cancer biomarkers / Anne Saumet and Charles-Henri Lecellier -- microRNA and pain / Atsushi Sakai and Hidenori Suzuki -- microRNA and epilepsy / Cristina R. Reschke and David C. Henshall -- microRNA and autism / Ayyappan Anitha and Ismail Thanseem -- microRNAs and neurogenerative diseases / Lifeng Qiu, Eng King Tan, and Li Zeng -- microRNAs and Fragile X Syndrome / Shi-Lung Lin -- The emerging role of mitomiRs in the pathophysiology of human disease / Filipe V. Duarte, Carlos M. Palmeira, and Anabela P. Rolo -- microRNAs in cerebrovascualar disease / Ondřej Volný, Linda Kašičková, Dominika Coufalová, Petra Cimflová, and Jan Novák -- microRNAs and cardiovascular remodeling / Koh Ono -- microRNAs in essential hypertension and blood pressure regulation / Francine Z. Marques and Fadi J. Charchar -- microRNA and pulmonary hypertension / Olivier Boucherat, François Potus, and Sébastien Bonnet -- microRNAs in diabetic kidney disease / Arthur C.K. Chung -- microRNA and kidney transplantation / Kíra Jelencsics and Rainer Oberbauer -- microRNA and wound healing / Jaideep Banerjee and Chandan K. Sen -- Essential role of microRNA in skin physiology and disease / Damjan Glavač and Metka Ravnik-Glavač -- microRNA and allergy / Ana Rebane -- microRNA in human reproduction / Iris Eisenberg, Noora Kotaja, Debra Goldman-Wohl, and Tal Imbar -- microRNAs and hepatitis B / Yutaka Naito, Yasuhito Tanaka, and Takahiro Ochiya -- Circulating microRNAs: the future of biomarkers in anti-doping field / Nicolas Leuenberger and Martial Saugy -- microRNA expression profiling: technologies, insights, and prospects / Christine Roden, Stephen Mastriano, Nayi Wang, and Jun Lu.
    Also available: Print – 2015
  • 2006 Springer Protocols
    edited by Shao-Yao Ying.
  • 2015 Springer
    Gaetano Santulli, editor.
    1. A fleeting glimpse inside microRNA, epigenetics and micropeptidomics -- 2. The microRNA machinery -- 3. microRNAs in mitochondria: an unexplored niche -- 4. microRNAs distinctively regulate vascular smooth muscle and endothelial cells: functional implications in angiogenesis, atherosclerosis, and in-stent restenosis -- 5. Mechanistic role of microRNAs in coupling lipid metabolism and atherosclerosis -- 6. microRNAs in pancreatic β-cell physiology -- 7. microRNA and cardiac regeneration -- 8. microRNAs and endometrial pathophysiology -- 9. microRNA and NF-kappa B -- 10. microRNAs: key players in hematopoiesis -- 11. Regulatory roles of miRNAs in aging -- 12. Computational prediction of microRNA targets
    Also available: Print – 2015
  • 2013 Springer Protocols
    edited by Stella K. Kantartzi.
    Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research. Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis.
  • 2014 Springer Protocols
    edited by Christoph Arenz.
    Part I. Reviews -- Pathway of miRNA maturation / Michael Sand -- MicroRNA maturation and human disease / Marlen Hesse and Christoph Arenz -- Approaches to the modulation of miRNA maturation / Valerie T. Tripp, Jaclyn R. McKenna, and Douglas D. Young -- Part II. Methods -- Expression profiling of components of the miRNA maturation machinery / Michael Sand and Marina Skrygan -- Primary MicroRNA processing assay reconstituted using recombinant Drosha and DGCR8 / Ian Barr and Feng Guo -- In vivo processing assay based on a dual-luciferase reporter system to evaluate DROSHA enzymatic activity / Vera Bilan, Danilo Allegra, Florian Kuchenbauer, and Daniel Mertens -- Assaying dicer-mediated miRNA maturation by means of fluorescent substrates / Marlen Hesse, Brian P. Davies, and Christoph Arenz -- Fluorescence correlation spectroscopy-based enzyme assay for human dicer / Eileen Magbanua and Ulrich Hahn -- Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification / Saskia Neubacher and Christoph Arenz -- Quantitative RT-PCR specific for precursor and mature miRNAs / Hannah Zöllner, Stephan A. Hahn, and Abdelouahid Maghnouj -- Cellular microRNA sensors based on luciferase reporters / Colleen M. Connelly and Alexander Deiters -- Identification of inhibitors of microRNA function from small molecule screens / Colleen M. Connelly and Alexander Deiters -- Inhibition of miRNA maturation by peptide nucleic acids / Concetta Avitabile, Enrica Fabbri, Nicoletta Bianchi, Roberto Gambari, and Alessandra Romanelli -- Molecular methods for validation of the biological activity of peptide nucleic acids targeting microRNAs / Eleonora Brognara, Enrica Fabbri, Nicoletta Bianchi, Alessia Finotti, Roberto Corradini, and Roberto Gambari -- Lentiviral overexpression of miRNAs / Hannah Zöllner, Stephan A. Hahn, and Abdelouahid Maghnouj.
  • 2014 Springer Protocols
    edited by Malik Yousef, Dabburiya Village, Israel, Jens Allmer, Izmir Institute of Technology, Izmir, Turkey.
    Introduction to microRNAs in biological systems / Ayse Elif Erson-Bensan -- The role of microRNAs in biological processes / Kemal Uğur Tüfekci, Ralph Leo Johan Meuwissen, and S̮̜ermin Genç -- The role of microRNAs in human diseases / Kemal Uğur Tüfekci [and three others] -- Introduction to bioinformatics / Tolga Can -- MicroRNA and noncoding RNA-related data sources / Patrizio Arrigo -- High-throughput approaches for MicroRNA expression analysis / Bala Gür Dedeoğlu -- Introduction to machine learning / Yalin Baş̧tanlar and Mustafa Özuysal -- Introduction to statistical methods for microRNA analysis / Gökmen Zararsiz and Erdal Coş̧gun -- Computational and bioinformatics methods for microRNA gene prediction / Jens Allmer -- Machine learning methods for microRNA gene prediction / Müş̧erref Duygu Saçar and Jens Allmer -- Functional, structural, and sequence studies of microRNA / Chanchal K. Mitra and Kalyani Korla -- Computational methods for microRNA target prediction / Hamid Hamzeiy, Jens Allmer, and Malik Yousef -- MicroRNA target and gene validation in viruses and bacteria / Debora Baroni and Patrizio Arrigo -- Gene reporter assay to validate microRNA targets in Drosophila S2 cells / Bünyamin Akgül and Çağdaş̧ Göktaş̧ -- Computational prediction of microRNA function and activity / Hasan Oğul -- Analysis of microRNA expression using machine learning / Henry Wirth [and three others] -- MicroRNA expression landscapes in stem cells, tissues, and cancer / Mehmet Volkan Çakir [and three others] -- Master regulators of posttranscriptional gene expression are subject to regulation / Syed Muhammad Hamid and Bünyamin Akgül -- Use of microRNAs in personalized medicine / Çiğir Biray Avci and Yusuf Baran.
  • 2009 Springer Protocols
    edited by Jeffrey A. Stuart.
    Biochemical isolation of mtDNA nucleoids from animal cells / D.F. Bogenhagen -- Analysis of mitochondrial DNA by two-dimensional agarose gel electrophoresis / A. Reyes [and others] -- Comparative purification strategies for Drosophila and human mitochondrial DNA replication proteins: DNA polymerase gamma and mitochondrial single-stranded DNA-binding protein / M.T. Oliveira and L.S. Kaguni -- Functional analysis of mutant mitochondrial DNA polymerase proteins involved in human disease / S.S. Chan and W.C. Copeland -- Preparation of human mitochondrial single-stranded DNA-binding protein / M.J. Longley, L.A. Smith and W.C. Copeland -- Methods for assessing binding of mitochondrial transcription factor A (TFAM) to DNA / A. Fukuoh and D. Kang -- Inducible expression in human cells, purification, and in vitro assays for the mitochondrial DNA helicase Twinkle / S. Goffart and H. Spelbrink -- Purification strategy for recombinant forms of the human mitochondrial DNA helicase / T.D. Ziebarth and L.S. Kaguni -- Methods for studying mitochondrial transcription termination with isolated components / P.L. Polosa [and others] -- Oxidative phosphorylation: synthesis of mitochondrially encoded proteins and assembly of individual structural subunits into functional holoenzyme complexes / S.C. Leary and F. Sasarman -- Reactive oxygen species production by mitochondria / A.J. Lambert and M.D. Brand -- Measuring mtDNA damage using a supercoiling-sensitive qPCR approach / S.W. Chan and J.Z. Chen -- Quantitative analysis of oxidized guanine, 8-oxoguanine, in mitochondrial DNA by immunofluorescence method / M. Ohno, S. Oka and Y. Nakabeppu -- In vitro measurement of DNA base excision repair in isolated mitochondria / M.M. Page and J.A. Stuart -- Targeting repair proteins to the mitochondria of mammalian cells through stable transfection, transient transfection, viral transduction, and TAT-mediated protein transduction / C.A. Koczor [and others] -- Construction and characterization of a cell line deficient in repair of mitochondrial, but not nuclear, oxidative DNA damage / S. Oka, M. Ohno and Y. Nakabeppu -- Mitochondrial DNA oxidative damage and mutagenesis in Saccharomyces cerevisiae / L.M. Griffiths [and others] -- Determination of DNA mutation load in human tissues using denaturing HPLC-based heteroduplex analysis / K.S. Lim, R.K. Naviaux and R.H. Haas -- Rapid identification of unknown heteroplasmic mitochondrial DNA mutations with mismatch-specific surveyor nuclease / S. Bannwarth, V. Procaccio and V. Paquis-Flucklinger -- Collection of isolated cells for studying mitochondrial DNA mutations within individual cells / Y. Kraytsberg [and others] -- Quantitative analysis of somatic mitochondrial DNA mutations by single-cell single-molecule PCR / Y. Kraytsberg [and others] -- Measuring DNA precursor pools in mitochondria / C.K. Mathews and L.J. Wheeler -- Establishment of human cell lines lacking mitochondrial DNA / K. Hashiguchi and Q.M. Zhang-Akiyama.
  • 2016 Springer Protocols
    edited by Matthew McKenzie
    Part I. Detection of mitochondrial DNA mutations and mitochondrial DNA damage -- Part II. Visualization of mitochondrial DNA and mitochondrial DNA nucleoids -- Part III. Analysis of mitochondrial DNA replication, mitochondrial DNA-encoded protein translation and mitochondrial DNA copy number -- Part IV. Modification of mitochondrial DNA -- Part V. Purification of proteins involved in mitochondrial DNA replication and transcription -- Index.
  • 2012 Springer Protocols
    edited by Yves Bigot.
    Transposable elements as tools for reshaping the genome : it is a huge world after all! / Solenne Bire and Florence Rouleaux-Bonnin -- Using and understanding RepeatMasker / Sébastien Tempel -- Roadmap for annotating transposable elements in eukaryote genomes / Emmanuelle Permal, Timothée Flutre, and Hadi Quesneville -- To detect and analyze sequence repeats whatever be their origin / Jacques Nicolas -- Exploring bacterial insertion sequences with ISfinder : objectives, uses, and future developments / P. Siguier [and others] -- Methods and software in NGS for TE analysis / Cristian Chaparro and Francois Sabot -- The application of LTR retrotransposons as molecular markers in plants / Alan H. Schulman [and others] -- Individual analysis of transposon polymorphisms by AFLP / Susanta K. Behura -- Construction of a library of random mutants in the spirochete Leptospira biflexa using a mariner transposon / Leyla Slamti and Mathieu Picardeau -- Ac-Ds solutions for rice insertion mutagenesis / Emmanuel Guiderdoni and Pascal Gantet -- Engineering the Caenorhabditis elegans genome by Mos1-induced transgene-instructed gene conversion / Valérie J.P. Robert -- Genome-wide manipulations of Drosophila melanogaster with transposons, Flp recombinase, and [phi]C31 integrase / Koen J.T. Venken and Hugo J. Bellen -- The Sleeping Beauty transposon toolbox / Ismahen Ammar, Zsuzsanna Izsvák, and Zoltán Ivics -- PiggyBac toolbox / Mario Di Matteo [and others] -- Insertion site pattern : global approach by linear amplification-mediated PCR and mass sequencing / Cynthia C. Bartholomae [and others] -- Comprehensive DNA methylation profiling of human repetitive DNA elements using an MeDIP-on-RepArray assay / Eric Gilson and Béatrice Hovard -- Novel approach for the development of new antibodies directed against transposase-derived proteins encoded by human neogenes / Ahmed Arnaoty [and others].
  • 2012 Springer
    Peter Hu, Madhuri Hegde, Patrick Alan Lennon, editors.
    Part 1. Current Molecular Laboratory Methods -- Specimen Collection, Handling, and Processing / Lindsy Hengesbach and John A. Gerlach -- DNA/RNA Isolation and Quantitation / Jing Wang -- Test Validation / Cindy McCloskey and S. Terence Dunn -- In Vitro Amplification Methods in Molecular Diagnostics / C. Alexander Valencia and Bradford Coffee -- Whole Genome Sequencing in the Clinical Laboratory / Tina Hambuch, Brad Sickler, Arnold Liao, Suneer Jain and Philip D. Cotter -- Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction / Applications to Factor VIII Gene in Hemophilia A and Endoglin/ACVRL1 Genes in Hereditary Hemorrhagic Telangiectasia / Juliana Purrazzella and Arupa Ganguly -- Reporting Clinical Molecular Genetic Laboratory Results / Victor Wei Zhang and Lora J. H. Bean -- Part 2. Clinical Molecular Infectious Disease Testing -- HIV-1 Genotyping and Virtual Phenotyping / Fangling Xu and Jianli Dong -- COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test, Version 2.0 / Tavat A. Buraruk and Charles E. Stager -- Hepatitis C Virus (HCV) Genotype Assay (LiPA) / Charles E. Stager and Tavat A. Buraruk -- COBAS® AmpliPrep/COBAS® TaqMan® HCV Test / Charles E. Stager and Tavat A. Buraruk -- Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction / Su S. Chen, Seema Hai, Margaret L. Gulley and Rajyalakshmi Luthra -- Quantification of BK Viral Load / Hai Wu and Jianli Dong -- Molecular Diagnosis and Epidemiology of Rabies / Rodney E. Rohde and Bonny C. Mayes -- Pyrosequencing for Bacterial Identification / Laura Millecker and Ruth Ann Luna -- Other Pathogens of Significant Public Health Concern / Chris L. McGowin, Rodney E. Rohde and Greg C. Whitlock -- Part 3. Clinical Molecular Oncology Testing -- Gene Rearrangements Testing / Siby Sebastian, Juan Du and Rizwan Naeem -- Mutation Analysis in Cancer / Keyur Pravinchandra Patel, John Galbincea and Rajyalakshmi Luthra -- Minimal Residual Disease / Wesley O. Greaves and Rajyalakshmi Luthra -- Pyrosequencing in Cancer / Shalini Verma, Bedia A. Barkoh and Rajyalakshmi Luthra -- Clinical Applications of Microarrays in Cancer / Marilyn M. Li, Ankita Patel and Xiaofeng Hu -- Basic Theories and Clinical Applications of Molecular Flow Cytometry / Hua-Kang Wu, Hongli Chen and Peter C. Hu -- Part 4. Clinical Molecular Pre/Postnatal Testing -- Molecular Prenatal Diagnostic Testing / Joanna Wiszniewska and Christine M. Eng -- PCR Based Diagnosis of Fragile X Syndrome / Daniel J. Dykas and Allen E. Bale -- Part 5. Human Identity Testing -- Microsatellite Testing Short Tandem Repeat Profile Generation / Robin DeVille Guidry and Laura Gahn -- Parentage / Laura Gahn and Robin DeVille Guidry -- Part 6. Clinical Molecular Training -- Diagnostic Molecular Training / Peter C. Hu, Patrick Alan Lennon and Madhuri R. Hegde -- Molecular Diagnostics Point of Care Testing Training / Chang W. Lee and Peter C. Hu.
  • 2010 Springer
    Srinivasan Yegnasubramanian, William B. Isaacs, editors.
    Genome-Scale Analysis of Data from High-Throughput Technologies / Sarah J. Wheelan -- Analysis of Inherited and Acquired Genetic Variation / Srinivasan Yegnasubramanian and William B. Isaacs -- Examining DNA-Protein Interactions with Genome-Wide Chromatin Immunoprecipitation Analysis / Esteban Ballestar and Manel Esteller -- Genome-Wide DNA Methylation Analysis in Cancer Research / Srinivasan Yegnasubramanian and William G. Nelson -- Use of Expression Microarrays in Cancer Research / Jun Luo and Yidong Chen -- Signal Sequencing for Gene Expression Profiling / Biaoyang Lin, Jeremy Wechsler and Leroy Hood -- Mass Spectrometry Based Proteomics in Cancer Research / Mohamad A. Abbani, Parag Mallick and Maryann S. Vogelsang -- Tissue Microarrays in Cancer Research / Toby C. Cornish and Angelo M. De Marzo.
  • 2013 Springer
    Gary Stephens, Sumiko Mochida, editors.
    "This book brings together leading international experts to discuss recent advances in the regulation of presynaptic voltage-gated Ca2+ channels (VGCCs), key signal transducers that represent one of the most widely modulated proteins in the body. It is now commonly accepted that presence of the VGCC complex defines an excitable cell. At a basic level, VGCCs transduce membrane potential change to chemical neurotransmitter release at presynaptic terminals. However, on-going scientific research, presented here, in areas including neuroscience, electrophysiology, pharmacology, biochemistry and, increasingly, proteomics, has revealed the widespread nature of modulation of the presynaptic VGCC complex. This book reviews and discusses the following topics: The fundamental role of the VGCC pore-forming CaVa subunit, and some of their binding partners, in presynaptic function and synaptic plasticity. Modulation of presynaptic CaVa subunits by auxiliary CaVb and a2d subunits and by their major interaction partners, such as active zone scaffolding proteins, synaptic proteins, G proteins and small GTPases, which, together, contribute to the VGCC proteome. Work at the cutting edge of research, including how direct electrophysiology recordings from presynaptic terminals and introduction of synthetic CaVa peptides into presynaptic terminals has expanded our knowledge of VGCC function. Evidence emerging over the last decade demonstrating that VGCC subunits represent bona fide molecular targets for therapeutic drug discovery. This development is illustrated by the introduction of the CaV2.2 blocker ziconotide, which represents an important proof-of-concept, but is best exemplified by the emergence of gabapentinoids, which bind the VGCC auxiliary a2d subunit, as first-line treatments for chronic neuropathic pain. Throughout, chapters are accompanied with illustrative Tables and Figure providing a useful and comprehensive summary of the current state-of-play in this area of significant therapeutic interest. Work described here also provides a solid basis for future research in this important area." -- Back cover.
  • 2002 ScienceDirect
    edited by I. Endo ... [et al.].
  • 2007 CRCnetBASE
    edited by Michel M.J. Modo, Jeff W.M. Bulte.
    Methods for labeling non-phagocytic cells with MR contrast agents / Joseph A. Frank, Stasia A. Anderson, & Ali S. Arbab -- Cellular imaging of cell transplants / Michel Modo & Jeff W.M. Bulte -- Cellular and molecular imaging of the diabetic pancreas / Zdravka Medarova & Anna Moore -- Functional cellular imaging with manganese / Vincent van Meir & Annemie van der Linden -- Translating promising experimental approaches to clinical trials / Adrian D. Nunn -- An outlook on cellular and molecular MR imaging / Michel Modo & Jeff W.M. Bulte. What is molecular and cellular imaging? / Michel Modo & Jeff W.M. Bulte -- The physicochemical principles of MR contrast agents / Peter Caravan -- Paramagnetic contract agents / Silvio Aime ... [et al.] -- Superparamagnetic contrast agents / Claire Corot ... [et al.] -- Physical mechanism and applications of cest contrast agents / Mike McMahon ... [et al.] -- Paracest contrast agents / A. Dean Sherry & Mark Woods -- Genetic approaches for modulating MRI contrast / Eric Ahrens, Williams F. Goins, Clinton S. Robinson -- Molecular and functional mr imaging of cancer / Michael A. Jacobs ... [et al.] -- Molecular imaging of atherosclerosis with magnetic resonance / Karen C. Briley-Saebo ... [et al.] -- Molecular imaging of apoptosis / Mikko Kettunen & Kevin M. Brindle -- Molecular imaging of reporter genes / Keren Ziv ... [et al.] -- Pharmacological MRI as a molecular imaging technique / Y. Iris Chen & Bruce Jenkins -- Cellular imaging of the liver using contrast agents / N. Cem Balci & Sukru Mehmet Erturk -- Cellular imaging of macrophage activity in infection and inflammation / Martin Rausch ... [et al.] -- Cellular imaging of macrophage activity in stroke / Ralph Weber & Mathias Hoehn -- Magnetic resonance imaging in developmental biology / Cyrus Papan, J. Michael Tyszka, Russell E. Jacobs --
  • 2005 Cold Spring Harb Lab Press
    SU Catalog (SearchWorks) Click LINK above for Print location/circulation status.
    meeting organized by Bruce Stillman and David Stewart.
    Cancer genetics and genomes -- DNA damage response -- Cancer biology and stem cells -- Telomeres, senescence, and aging -- Animal models for cancer -- Gene expression and cancer -- Tumor responses to microenvironment -- Angiogenesis -- Discovering cancer targets -- Therapeutic approaches.
  • 2012 Springer
    Michał Witt, Małgorzata Dawidowska, Tomasz Szczepánski, editors.
    Part 1. Molecular Biology of Selected Hemato-oncologic Diseases -- Molecular Biology of Acute Lymphoblastic Leukemia / Małgorzata Dawidowska, Monika D. Kraszewska, Katarzyna Derwich and Tomasz Szczepański -- Genetic Mechanisms and Molecular Markers of Neoplastic Transformation in Acute Myeloid Leukemia / Agata A. Filip, Marta Libura, Sebastian Giebel and Olga Haus -- Molecular Pathogenesis of Aggressive B-cell Lymphomas / Przemysław Juszczyński and Krzysztof Warzocha -- Molecular Biology and Classification of Multiple Myeloma / Anna Dmoszyńska and Norbert Grząśko -- Chronic Lymphocytic Leukemia / Anton W. Langerak and Yorick Sandberg -- Molecular Biology of Chronic Myeloid Leukemia / Tomasz Sacha, Kajetana Foryciarz and Aleksander B. Skotnicki -- Molecular Biology of Myelodysplastic Syndromes / Francesc Solé -- Myeloproliferative Neoplasms / Andrzej Hellmann, Maria Bieniaszewska, Witold Prejzner and Aleksandra Leszczyńska -- Part 2. Diagnostics and Monitoring of Therapy of Hemato-oncologic Deseases -- Flow Cytometric Immunophenotyping as Diagnostic Tool of Hematopoietic Malignancies / Łukasz Sędek, Juan Flores-Montero, Joanna Bulsa, Susana Barrena and Julia Almeida, et al. -- Cytogenetics in Hematology / Olga Haus, Anna Poluha and Katarzyna Skonieczka -- Monitoring of Minimal Residual Disease in Acute Lymphoblastic Leukemia / Tomasz Szczepański, Małgorzata Dawidowska and Katarzyna Derwich -- Gene Expression Profiling in Hematologic Malignancies / Przemysław Juszczyński, Bjoern Chapuy, Małgorzata Szczepaniak and Krzysztof Warzocha -- Part 3. Molecular Procedures Involved in Hematopoietic Stem Cell Transplantation -- Allogeneic Transplantation of Hematopoietic Stem Cells / Jacek Wachowiak -- Genetic Basis of Donor-Recipient Matching in Allogeneic Transplantation of Hematopoietic Stem Cells / Jacek Nowak and Jacek Wachowiak -- Chimerism Following Allogeneic Transplantation of Hematopoietic Stem Cells / Małgorzata Dawidowska, Katarzyna Guz, Ewa Brojer, Jacek Wachowiak and Michał Witt -- Peritransplantation Monitoring of Minimal Residual Disease in Acute Lymphoblastic Leukemia / Małgorzata Dawidowska, Katarzyna Derwich and Tomasz Szczepański -- Biobanks of Cellular Material / Wiesław Wiktor-Jędrzejczak and Elżbieta Urbanowska -- Part 4. Molecular Diagnostic Protocols -- Biobanking of Cellular Material / Anna Poluha and Elżbieta Urbanowska -- Isolation of Mononuclear Cells from Human Blood and Bone Marrow by Density Gradient Centrifugation / Małgorzata Dawidowska -- Molecular Techniques Commonly Used in Hemato-oncology / Monika D. Kraszewska and Ewa Ziętkiewicz -- Genetic Methods of HLA Typing / Jacek Nowak, Renata Mika-Witkowska and Elżbieta Graczyk-Pol -- Post-Transplant Chimerism Analysis Through STR-PCR and RQ-PCR / Małgorzata Dawidowska, Katarzyna Guz, Agnieszka Orzińska, Justyna Smolarczyk-Wodzyńska and Monika D. Kraszewska, et al. -- Analysis of Minimal Residual Disease with the Use of Rearrangements of Ig/TCR Genes Through RQ-PCR / Małgorzata Dawidowska, Vincent H. J. van der Velden, Michał Witt and Tomasz Szczepański -- Molecular Diagnostics of Acute Myeloblastic Leukemia / Marta Libura, Agata A. Filip and Olga Haus -- Assessment of the Presence and the Level of BCR-ABL Fusion Gene Expression and Mutational Status in ABL Kinase Domain / Sylwia Czekalska, Magdalena Zawada and Izabela Florek -- Studies of Rearrangements and Somatic Hypermutation of IGHV Genes in Chronic Lymphocytic Leukemia / Anton W. Langerak, Richard Rosenquist, Paolo Ghia, Chrysoula Belessi and Kostas Stamatopoulos, et al. -- Molecular Biology Methods in the Diagnosis of Multiple Myeloma / Anna Dmoszyńska and Sylwia Chocholska -- Molecular Methods in Myeloproliferative Neoplasms / Aleksandra Leszczyńska, Witold Prejzner, Maria Bieniaszewska and Andrzej Hellmann -- Informed Consent for Participation in Research Project / Małgorzata Dawidowska and Michał Witt.
  • 2007 Springer
    edited by Peter Csermely, László Vígh.
    Protein misassembly: macromolecular crowding and molecular chaperones / R. John Ellis -- The cellular "networking" of mammalian HSP27 and its functions in the control of protein folding, redox state and apoptosis / André-Patrick Arrigo -- Molecular interaction network of the HSP90 chaperone system / Rongmin Zhao and Walid A. Houry -- Organization of the functions and components of the endoplasmic reticulum / Yuichiro Shimizu and Linda M. Hendershot -- Molecular crime and cellular punishment: active detoxification of misfolded and aggregated proteins in the cell by the chaperone and protease networks / Marie-Pierre Hinault and Pierre Goloubinoff -- Chaperones as parts of cellular networks / Peter Csermely, Csaba Söti and Gregory L. Blatch -- Chaperones as parts of organelle networks / György Szabadkai and Rosario Rizzuto -- Heat shock factor 1 as a coordinator of stress and developmental pathways / Julius Anckar and Lea Sistonen -- Chaperone regulation of the heat shock protein response / Richard Voellmy and Frank Boellmann -- Mechanisms of activation and regulation of the heat shock-sensitive signaling pathways / Sébastien Ian Nadeau and Jacques Landry -- Membrane-regulated stress response: a theoretical and practical approach / László Vígh ... [et al.] -- Beyond the lipid hypothesis: mechanisms underlying phenotypic plasticity in inducible cold tolerance / Scott A.L. Hayward ... [et al.] -- Trehalose as a "chemical chaperone:" fact and fantasy / John H. Crowe -- Chaperones as part of immune networks / Zoltán Prohászka -- The stress of misfolded proteins: C. elegans models for neurodegenerative disease and aging / Heather R. Brignull, James F. Morley and Richard I. Morimoto -- HSP90 and developmental networks / Suzannah Rutherford, Jennifer R. Knapp and Peter Csermely.
    Also available: Print – 2007
  • 2008
    [edited by] John Mendelsohn ... [et al.].
    Status: Not Checked OutLane Catalog Record
  • 2015 ClinicalKey
    [edited by] John Mendelsohn, Joe W. Gray, Peter M. Howley, Mark A. Israel, Craig B. Thompson.
    Cancer : a genetic disorder / William C. Hahn, Robert A. Weinberg -- Oncogenes and signal transduction / Luca Grumolato, Stuart A. Aaronson -- Tumor suppressor genes / Nelson E. Brown, Philip W. Hinds -- DNA repair pathways and human cancer / Alan D. D'Andrea -- Epigenetics and cancer / Stephen B. Baylin -- Infectious agents and cancer / Peter M. Howley -- Environmental carcinogenesis / Erika L. Abel, John DiGiovanni -- Animal models : flies, fish, and yeast / Elspeth Payne, A. Thomas Look -- Genetic mouse models of cancer / Monte M. Winslow, Tyler Jacks -- Cancer stem cells and the microenvironment / Hasan Korkaya, April Davis, Max S. Wicha -- Regulation of the cell cycle / Yan Li, Olena Barbash, J. Alan Diehl -- Cell growth / David A. Guertin, David M. Sabatini -- Metabolism of cell growth and proliferation / Lydia W.S. Finley, Craig B. Thompson -- Apoptosis, necrosis, and autophagy / Eileen White, Douglas R. Green, Anthony G. Letai -- Cellular senescence / Jessica E. Bolden, Scott W. Lowe -- Role of the microenvironment in tumor initiation, progression, and metastasis / Cyrus M. Ghajar, Ana Luísa Correia, Mina J. Bissell -- Tumor angiogenesis / Brian Keith, M. Celeste Simon -- Invasion and metastasis / Swarnali Acharyya, Lynn Matrisian, Danny R. Welch, Joan Massagué -- Inflammation and cancer / Daniela F. Quail, Johanna A. Joyce -- Cancer systems biology : the future / Andrea Califano -- Biomarkers for assessing risk of cancer / Xifeng Wu, Jian Gu -- Protein biomarkers for detecting cancer : molecular screening / Susan Fisher, H. Ewa Witkowska -- Technology of analyzing nucleic acids in Cancer / Elaine R. Mardis -- Understanding and using information about cancer genomes / Paul T. Spellman, Josh Stuart, Joe W. Gray -- High-content analysis with cellular and tissue systems biology : a bridge between cancer cell biology and tissue-based diagnostics / Albert Gough, Timothy Lezon, James R. Faeder, Chakra Chennubhotla, Robert F. Murphy, Rebecca Critchley-Thorne, D. Lansing Taylor -- Molecular genetics of acute lymphoblastic leukemia / Eric S. Schafer, Judith Margolin, David G. Poplack, Karen R. Rabin -- Molecular biology of childhood neoplasms / David Malkin -- Biology of adult myelocytic leukemia and myelodysplasia / Sean M. Post, Hagop Kantarjian, Alfonso Quintás-Cardama -- Lymphoma / Owen A. O'Connor, Jennifer Amengual, Changchun Deng, Ahmed Sawas, Bachir Alobeid, Daniela Hoehn, Govind Bhagat -- Multiple myeloma / Giovanni Tonon, Kenneth C. Anderson -- Molecular mechanisms of esophageal cancer / Mario D. Terán, Ronan Kelly, Malcolm V. Brock -- Molecular basis of lung cancer / Jill E. Larsen, Ramaswamy Govindan, John D. Minna -- Molecular pathogenesis of head and neck cancer / Matthew L. Hedberg, Jennifer R. Grandis -- Colon and rectal cancer / Eric R. Fearon -- Molecular pathogenesis of pancreatic adenocarcinoma / Rachna T. Shroff, James L. Abbruzzese -- Molecular biology of breast cancer / Adrian V. Lee, Steffi Oesterreich, Nancy E. Davidson -- Molecular pathogenesis of ovarian cancer / Robert C. Bast Jr., Ignacio Romero, Gordon B. Mills -- Molecular basis of prostate cancer / Long-Cheng Li, Andrew C. Hsieh, Davide Ruggero, Kirsten L. Greene, Peter R. Carroll -- Molecular abnormalities in kidney cancer / Sabina Signoretti, Toni K. Choueiri, William G. Kaelin Jr. -- Biology of primary brain tumors / Matthew C. Havrda, Mark A. Israel -- Epithelial skin cancer / Adam B. Glick, Stuart H. Yuspa -- Molecular basis for treating cutaneous molecular / Marc S. Ernstoff -- Thyroid cancer / Steven I. Sherman -- Soft tissue sarcomas / Julie D.R. Reimann, Christopher D.M. Fletcher -- From bench to bedside with targeted therapies / John Mendelsohn -- Cancer therapeutics / William N. Hait, Eric Rubin, Joseph R. Bertino -- Natural and acquired resistance to cancer therapies / Branimir I. Sikic -- Phase I trials today / Daniel Morgensztern, Patricia M. LoRusso, Scott A. Boerner, Roy S. Herbst, Joseph Paul Eder -- Pharmacogenomics / Peter H. O'Donnell, Mark J. Ratain -- Monoclonal antibodies for the treatment of cancer / Louis M. Weiner, Rishi Surana -- Cancer and the cellular immune response / Patrick Hwu, Gregory Lizée, Yong-Jun Liu -- Cancer immunotherapy with vaccines and checkpoint blockade / Drew Pardoll -- Interferons / Ernest C. Borden -- Gene therapy and oncolytic viruses / Tanja Meyer Tamguney, W. Michael Korn, Frank McCormick -- RNA as a therapeutic molecule / George Adrian Calin, Carlo Maria Croce -- Heat shock protein 90 and the proteasome : housekeeping proteins that are also molecular targets for cancer therapy / Len Neckers, Mehdi Mollapour -- Hematopoietic growth factors and cytokines / Suhendan Ekmekcioglu, Razelle Kurzrock, Elizabeth A. Grimm -- Cancer chemoprevention / Scott M. Lippman, J. Jack Lee -- Molecular pathology / Christopher L. Corless.
  • 2010 Springer
    Roland Martin, Andreas Lutterotti (eds.).
    Also available: Print – 2010
  • 2010 Springer
    edited by Francis X. McCormack, Ralph J. Panos, Bruce C. Trapnell.
    Clinical approach to rare lung diseases -- Clinical trials for rare lung diseases -- Idiopathic and familial pulmonary arterial hypertension -- Lymphangioleiomyomatosis -- Autoimmune pulmonary alveolar proteinosis -- Mutations in surfactant protein C and interstitial lung disease -- Hereditary haemorrhagic telangiectasia -- Hermansky-Pudlak Syndrome -- Alpha-1 antitrypsin deficiency -- Marfan syndrome -- Surfactant deficiency disorders: SP-B and ABCA3 -- Pulmonary capillary hemangiomatosis -- Anti-glomerular basement disease: Goodpasture's Syndrome -- Primary ciliary dyskinesia -- Pulmonary alveolar microlithiasis -- Cystic fibrosis -- Pulmonary Langerhans' cell histiocytosis: advances in the understanding of a true dendritic cell lung disease -- Sarcoidosis -- Scleroderma lung disease.
  • 2008 Springer Protocols
    edited by Andreas Marx and Oliver Seitz.
    Basic principles of fluorescence and energy transfer / Larry E. Morrison -- Multiplex detection of mutations / David S. Perlin, Sergey Balashov, and Steven Park -- Detection of salmonella species in foodstuffs / Arvind A. Bhagwat ... [et al.] -- Identification of homozygous transgenic mice by genomic real-time PCR / Gunter Schmidtke and Marcus Groettrup -- Quantitative analysis of gene expression relative to 18S rRNA in carcinoma samples using the LightCycler instrument and a SYBR greenI-based assay : determining FAT10 mRNA levels in hepatocellular carcinoma / Sebastian Lukasiak ... [et al.] -- Absolute quantitation of feline leukemia virus proviral DNA and viral RNA loads by TaqMan real-time PCR and RT-PCR / Valentino Cattori and Regina Hofmann-Lehmann -- High-throughput real-time PCR / Thomas D. Schmittgen, Eun Joo Lee, and Jinmai Jiang -- Design and use of scorpions fluorescent signalling molecules / Rachael Carters ... [et al.] -- Hybridization probe pairs and single-labeled probes : an alternative approach for genotyping and quantification / Thomas Froehlich and Oliver Geulen -- EasyBeacons for the detection of methylation status of single CpG duplets / Ulf B. Christensen -- Quenched autoligation probes / Adam P. Silverman, Hiroshi Abe, and Eric T. Kool -- HyBeacon probes for rapid DNA sequence detection and allele discrimination / David J. French ... [et al.] -- FIT-probes in real-time PCR / Elke Socher and Oliver Seitz -- SNP genotyping by unlabeled probe melting analysis / Maria Erali, Robert Palais, and Carl Wittwer -- Molecular beacons for protein-DNA interaction studies / Jun Li ... [et al.] -- DNA polymerase profiling / D. Summerer -- Modular reporter hairpin ribozymes for analyzing molecular interactions / S. Hani Najafi-Shoushtari and Michael Famulok -- Screening of molecular interactions using reporter hammerhead ribozymes / Jörg S. Hartig and Michael Famulok.
  • v. 1-2, 1985-86.
    George Feuer, Felix A. De la Iglesia.
    Status: Not Checked OutLane Catalog Record
  • 2013 ClinicalKey
    David P. Clark, Nanette J. Pazdernik.
    Unit 1. Basic Chemical and Biological Principles -- Unit 2. The Genome -- Unit 3. The Central Dogma of Molecular Biology -- Unit 4. Regulating Gene Expression -- Unit 5. Subcellular Life Forms -- Unit 6. Changing the DNA Blueprint.
  • 2002 CRCnetBASE
    edited by Heinz D. Osiewacz.
  • 2005 Springer
    by Wolfgang Arthur Schulz.
  • 2006 Springer
    Markus J. Tamás, Enrico Martinoia (eds.).
  • 2008
    Bruce Alberts ... [et al.] ; with problems by John Wilson ... [et al.].
    Status: Not Checked OutLane Catalog Record
  • 2015
    Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter ; with problems by John Wilson, Tim Hunt.
    Status: Not Checked OutLane Catalog Record
    "As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts. As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced. Protein structures better illustrate structure-function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing open-ended questions highlighting "What We Don't Know," introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text. Thought-provoking end-of-chapter questions have been expanded to all chapters, including questions on developmental biology, tissues and stem cells, the immune system, and pathogens."-- Provided by publisher.
  • 2002 NCBI Bookshelf, Fulltext accessible by its search box
    Bruce Alberts ... [et al.].
    Also available: Print – 2002
    Also available: Print – 2002
  • 2008
    Bruce Alberts ... [et al.] ; with problems by John Wilson, Tim Hunt.
    Status: Not Checked OutLane Catalog Record
    Cells and genomes -- Cell chemistry and biosynthesis -- Proteins -- DNA, chromosomes, and genomes -- DNA replication, repair, and recombination -- How cells read the genome: from DNA to protein -- Control of gene expression -- Manipulating proteins, DNA, and RNA -- Visualizing cells -- Membrane structure -- Membrane transport of small molecules and the electrical properties of membranes -- Intracellular compartments and protein sorting -- Intracellular vesicular traffic -- Energy conversion: mitochondria and chloroplasts -- Mechanisms of cell communication -- The cytoskeleton -- The cell cycle -- Apoptosis -- Cell junctions, cell adhesion, and the extracellular matrix -- Cancer -- Sexual reproduction: meiosis, germ cells, and fertilization -- Development of multicellular organisms -- Specialized tissues, stem cells, and tissue renewal -- Pathogens, infection, and innate immunity -- The adaptive immune system. Tables: The genetic code, amino acids.
  • 2008
    James D. Watson, Tania A. Baker, Stephen P. Bell, Alexander Gann, Michael Levine, Richard Losick.
    Status: Not Checked OutLane Catalog Record
    The Mendelian view of the world -- Nucleic acids convey genetic information -- The importance of weak chemical interactions -- The importance of high-energy bonds -- Weak and strong bonds determine macromolecular structure -- The structures of DNA and RNA -- Genome structure, chromatin, and the nucleosome -- The replication of DNA -- The mutability and repair of DNA -- Homologous recombination at the molecular level -- Site-specific recombination and transposition of DNA -- Mechanisms of transcription -- RNA splicing -- Translation -- The genetic code -- Transcriptional regulation in prokaryotes -- Transcriptional regulation in eukaryotes -- Regulatory RNAs -- Gene regulation in development and evolution -- Genome analysis and systems biology -- Techniques of molecular biology -- Model organisms.
  • 2005 Springer
    [edited by] Tally Naveh-Many.
  • 2010 Springer
    Sunil K. Lal, editor.
    The SARS outbreak in 2002 caused unprecedented devastation and exhibited unusually high rates of morbidity and mortality. For the SARS coronavirus, the transmission vehicle was man. What added to the spread was man's convenient, rapid and affordable ways of travel that caused the virus to spread globally in a short span of time. The SARS coronavirus jumped the species barrier, similar to many recent zoonotic diseases, avian flu, swine flu, Nipah and encephalitis virus. It is extremely important for us to understand the enemy before it strikes again in order to be able to combat such outbreaks as and when they occur. Molecular mechanisms that the virus exploits within its host and that result in infection and pathogenesis are the key to being able to block its transmission cycle. In this book, leading scientists from around the globe have discussed molecular aspects of viral entry, replication, viral gene expression, pathogenesis and host immune response against the SARS coronavirus.
  • 2014 Springer
    Nalini M. Rajamannan, editor.
    This book presents the cellular details and the basic molecular biology approaches for understanding cardiac valvular disease. It provides novel cellular targets for developing future clinical trials and comprehensive clinical discussion of this expanding field. Molecular Biology of Valvular Heart Disease will guide physicians on the potential medical therapies to treat valvular heart disease while providing a platform for cellular targeting of valvular heart disease. Residents and fellows in cardiology, interventional cardiology, cardiac surgery, and current and future valve biologists alike will find this book to be an invaluable resource for developing further interest and understanding of the cellular basis for cardiac valvular disease treatment.
  • 2012 ScienceDirect
    2012 ClinicalKey
    by Susan Carson, Heather B. Miller, D. Scott Witherow.
  • 2008 Springer Protocols
    edited by John M. Walker, Ralph Rapley.
    Also available: Print – 2008
  • 2013 Springer
    Norma Allewell, Linda O. Narhi, Ivan Rayment (eds.).
    This volume of the series Biophysics for the Life Sciences focuses on the conceptual framework and major research tools of contemporary molecular biophysics. It is designed to enable non-specialists -both students and professionals in other fields - to understand how these approaches can be used across the biosciences and in medicine, agriculture, biotechnology, pharmaceutical development and other fields. The scope of this volume is appropriate for advanced undergraduate and graduate courses in biophysics and biophysical chemistry. The book begins with an overview of the development of molecular biophysics and a brief survey of structural, physical, and chemical principles. Subsequent chapters written by experts present, with examples, the major experimental methods: optical spectroscopy, X-ray and neutron diffraction and scattering, nuclear magnetic resonance, electron paramagnetic resonance, mass spectrometry, and single molecule methods. The relationship between the biophysical properties of biological macromolecules and their roles as molecular machines is emphasized throughout and illustrated with three examples DNA helicases, rotary motor ATPases, and myosin.The concluding chapter discusses future prospects in X-ray and neutron scattering, mass spectrometry, and pharmaceutical development.
  • 2006 CRCnetBASE
    edited by David Warshawsky, Joseph R. Landolph, Jr.
    Carcinogens and mutagens -- Whole animal carcinogenicity bioassays -- Molecular mechanisms of action of selected organic carcinogens -- Binding of carinogens to DNA and covalent adducts and DNA damage - PAH, aromatic amines, nitro-aromatic compounds, and halogenated compounds -- Cellular oncogenes and carcinogenesis -- Tumor suppressor genes -- Hepatitis B, Hepatitis C, and hepatocellular carcinoma -- Oncongenesis in transgenic and knockout mice -- Chemically induced morphological and neoplastic transformation in C3H/10T1/2 C1 8 mouse embryo cells -- Radiation carcinogenesis -- DNA microarrays and computational analysis of DNA microarray data in cancer research -- Application of proteomics in basic biological sciences and cancer -- Overview of human cancer induction and human exposure to carcinogens -- Complex mixtures of chemical carcinogens: principles of action and human cancer -- Lung cancer -- Breast cancer -- Cancer of the prostate - Mechanisms of molecular carcinogenesis -- Skin cancer: epidemiology, clinical manifestations, and genetic and molecular aspects -- Cell and molecular biology of cancer of the brain --Thyroid cancer: molecular biology and clinical aspects -- Chemoprevention of chemical carcinogenesis and human cancer -- Carcinogen biomarkers and exposure assessment -- Risk assessment for chemical carcinogens -- Prevention and regulation approaches to carcinogens.
    Also available: Print – 2006
  • 2013
    Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott.
    Status: Not Checked OutLane Catalog Record
    Overview: Molecular Cell Biology presents the key concepts in cell biology and their experimental underpinnings. The authors, all world-class researchers and teachers, incorporate medically relevant examples where appropriate to help illustrate the connections between cell biology and health and human disease. As always, a hallmark of MCB is the use of experiments to engage students in the history of cell biology and the research that has contributed to the field. The book contains predominantly color illustrations, with some black-and-white illustrations.
  • 2000 NCBI Bookshelf
    Harvey Lodish ... [et al.].
    Also available: Print – 2000
  • 2002 ScienceDirect
    edited by Philippe Sansonetti.
    Also available: Print – 2002
  • 2010 Springer
    edited by Andreas Luch.
  • 2009 Springer
    edited by Andreas Luch.
  • 2002 Springer Protocols
    edited by Yao-Shan Fan.
  • 2011 Springer
    Naranjan S. Dhalla, Makoto Nagano, Bohuslav Ostadal, editors.
    Calcium Overloading-Induced Oxidative Stress-Mediated Cellular and Subcellular Remodeling -- Intracellular MMP-2: Role in Normal and Diseased Hearts -- Control of the Mesenchymal-Derived Cell Phenotype by Ski and Meox2: A Putative Mechanism for Postdevelopmental Phenoconversion -- Elevated Na+-H+ Exchanger Expression and Its Role in Myocardial Dise -- Impact of Perinatal Chronic Hypoxia on Cardiac Tolerance to Acute Ischemia -- Stem Cell, MicroRNA and Redox Cycling -- Cardiac Cell Therapy: Present and Future -- Macroscopic and Microscopic Aspects of Cardiac Dysfunction in Congestive Heart Failure -- Cardiac Dysfunction and Metabolism: Unravelling the Molecular Cross-Talk -- Metabolic Remodelling of the Hypertrophied Heart -- Mechanisms for the Regulation of Phospholipase C Gene Expression in Cardiac Hypertrophy -- Molecular Changes in Fatty Acid Oxidation in the Failing Heart -- Cardiolipin Metabolism in Experimental and Human Heart Failure -- Mechanisms Underlying Development of Cardiomyocyte Hypertrophy via Na-H Exchange Stimulation -- The Structural Basis of Cardiac Dysfunction in Human Heart Failure -- Hormonal Mechanisms of Cardiac Remodeling in Heart Failure -- Modulators of Remodeling After Myocardial Infarction -- Phosphodiesterase-5 Inhibitors in Protection Against Doxorubicin-Induced Cardiomyopathy -- Molecular Basis of Pulmonary Hypertension in Left Heart Failure -- Modulation of Gi Protein Expression in Hypertension: Molecular Mechanisms -- Vascular Dysfunction in Heart Disease -- Cellular and Molecular Mechanisms Associated with Salicylate Inhibition of Intimal Hyperplasia Following Balloon Catheter-Induced Vascular Injury -- Involvement of Growth Factor Receptor and Nonreceptor Protein Tyrosine Kinases in Endothelin-1 and Angiotensin II-Induced Signaling Pathways in the Cardiovascular System -- Sarco/Endoplasmic Reticulum Ca2+ Pump Damage by Oxidative Stress: Implications for Ca2+ Entry -- Obesity-Hypertension: Leptin as the Common Link to Cardiovascular and Renal Dysregulation -- Cardiorenal Protection in Diabetes Mellitus -- Molecular Mechanisms in the Pathogenesis of Diabetic Cardiomyopathy.
  • 2013 Springer Protocols
    edited by Cristina Has and Cassian Sitaru.
    Molecular dermatology comes of age / Cristina Has and Cassian Sitaru -- Molecular diagnosis of autoimmune blistering diseases / Daisuke Tsuruta [and others] -- Molecular diagnosis of genodermatoses / Vesarat Wessagowit -- Three-dimensional visualization of the molecular architecture of cell-cell junctions in situ by cryo-electron tomography of vitreous sections / Ashraf Al-Amoudi and Achilleas S. Frangakis -- 3D visualization of epidermal Langerhans cells / Akiharu Kubo, Keisuke Nagao, and Masayuki Amagai -- In vivo imaging of lymph node lymphangiogenesis by immuno-positron emission tomography / Viviane Mumprecht and Michael Detmar -- Optical imaging of HPV infection in a murine model / Rhonda C. Kines [and others] -- Laser scanning microscopy approach for semiquantitation of in vitro dermal particle penetration / Hagar I. Labouta, Ulrich F. Schaefer, and Marc Schneider -- Analysis of cutaneous somatic mosaicism / Anna M.G. Pasmooij and Marcel F. Jonkman -- Global proteome analyses of SILAC-gabeled skin cells / Adrian Sprenger [and others] -- MicroRNA profiling during human keratinocyte differentiation using a quantitative real-time PCR method / Janosch Hildebrand [and others] -- Cell death in the skin : how to study its quality and quantity? / Roman Makarov [and others] -- In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus / Ken Ishii and Masayuki Amagai -- Induction of granulocyte-dependent dermal-epidermal separation by autoantibodies ex vivo / Mircea Teodor Chiriac -- Analysis of collective invasion of carcinoma cells in a 3D organotypic model / Jean Albrengues, Gerrino Meneguzzi, and Cedric Gaggioli -- Isolation of melanoma tumor-initiating cells from surgical tissues / Alexander D. Boiko -- Detection, enumeration, and characterization of immune cells infiltrating melanoma tumors / Benjamin Toh [and others] -- Analysis of cell movement between skin and other anatomical sites in vivo using photoconvertible fluorescent protein "Kaede"-transgenic mice / Michio Tomura and Kenji Kabashima -- Reconstitution of skin fibrosis development using a tissue engineering approach / Véronique J. Moulin -- Bioengineered skin humanized model of psoriasis / Marta Carretero, Sara Guerrero-Aspizua, and Marcela Del Río -- Induction of contact hypersensitivity in the mouse model / Stefan F. Martin --Generation of functional multipotent keratinocytes from mouse induced and pluripotent stem cells / Ganna Bilousova and Dennis R. Roop -- RNAi-mediated gene function analysis in skin / Slobodan Beronja and Elaine Fuchs -- Mouse models of autoimmune blistering diseases induced by the passive transfer of antibodies / Wataru Nishie -- induction of experimental epidermolysis bullosa acquisita by immunization with murine collagen VII / Alina Sesarman and Cassian Sitaru -- Animal model for cutaneous leishmaniasis / Michael R. Fischer [and others] -- Model for generation of large numbers of primary inflammatory skin-derived neutrophils and macrophages / Dominik John, Michael R. Fischer, and Esther von Stebut -- Zebrafish as a model system to study heritable skin diseases / Qiaoli Li and Jouni Uitto -- Cell- and protein-based therapy approaches for epidermolysis bullosa / Alexander Nyström, Leena Bruckner-Tuderman, and Johannes S. Kern -- RNA trans-splicing for genodermatoses / Johann W. Bauer [and others].
  • 2005 Springer
    by Gerrit J. Viljoen, Louis H. Nel and John R. Crowther.
  • 2014 Springer
    W. Edward Highsmith, Jr., editor.
    Molecular Diagnostics: 12 Tests That Changed Everything focuses on specific laboratory tests and emphasizes how the availability of these tests has altered how clinicians treat their patients. Presented as a standard outline, each chapter focuses on a specific molecular test and provides background on the test and its clinical applications. Continuing with some discussion on how the test is done, interpreted, and used clinically, each chapter then concludes with a discussion of how that test has changed the way medicine is practiced with respect to the disease or condition in question. Authored by renowned experts in the field, Molecular Diagnostics: 12 Tests That Changed Everything is a valuable resource for pathologists, pathology residents, laboratory directors, development personnel, lab medicine fellows and those working in the broad area of oncology, infectious disease and genetics.
  • 2006 Springer
    edited by William B. Coleman and Gregory J. Tsongalis.
    Pt. I. Introduction. An historical perspective on the clinical diagnostic laboratory / Robert E. Moore -- Pt. II. Basic molecular biology. An overview of nucleic acid chemistry, structure, and function: the foundations of molecular biology / William B. Coleman -- Extraction of nucleic acids / Paul N. Bogner and Anthony A. Killeen -- Nucleic acid blotting techniques: theory and practice / Terry Amiss and Sharon collins Presnell -- The polymerase chain reaction / William B. Coleman and Gregory J. Tsongalis -- Bioinformatics: computer-based approaches to genetic analysis / Sharon L. Ricketts -- Pt. III. Molecular diagnostic technologies. PCR-based methods for mutation detection / Ian M. Frayling, Emma Monk, and Rachel Butler -- Alternative methods for amplified nucleic acid testing / Deborah A. Payne and Laurie E. Sower -- Electrophoretic methods for mutation detection and DNA sequencing / W. Edward Highsmith, Jr. -- Single-nucleotide polymorphisms: testing DNA variation for disease association / Ulrich Broeckel and Martin J. Hessner -- Microarray approaches to gene expression analysis / David Neil Hayes and Matthew Meyerson -- Methods for analysis of DNA methylation / Alexander Dobrovic -- Pt. IV. Other clinical diagnostic technologies / Flow Cytometry / Joseph A. DiGiuseppe -- Medical cytogenetics / Martha B. Kaegle -- Fluorescence In situ hybridization: a major milestone in luminous cytogenetics / Suneel D. Mundle and Robert J. Koska -- Immunohistochemistry / John Hunt ... [et al.] -- Lser capture microdissection / C. Robert Bagnell, Jr. -- Pt. V. Quality assurance in the molecular diagnostics laboratory. Framework for quality assurance in molecular diagnostics / Marlene Sabbath-Solitare, Selwyn J. Baptist, and Teresita Cuyegkeng Redondo -- Verification of molecular assays / Brent L. Seaton -- Standards and standardization of molecular diagnostics / John P. Jakupciak and Catherine D. O'Connell -- Laboratory-developed tests in molecular diagnostics / Andrea Ferreira-Gonzalez and Carleton T. Garrett -- Pt. VI. Applications of molecular diagnostics for genetic diseases. An overview of molecular genetics / Elaine Weidenhammer and Gregory J. Tsongalis -- Genetic basis of neurologic neuromuscular diseases / Narasimhan Nagan ... [et al.] -- Molecular mechanisms of endocrine disorders / Amy Potter and John A. Phillips III -- Molecular pathogenesis of cardiovascular disease / Alan H.B. Wu -- Molecular diagnostics in coagulation / Enrique Ballesteros -- Cystic fibrosis / Eugene H. Lewis III ... [et al.] -- Prenatal genotyping for identification of identification of fetuses at risk for immune cytopenic disorders / Martin J. Hessner and Brian R. Curtis -- Personalized medicine / Karen McCullough -- Pt. VII. Applications of molecular diagnostics for human cancers. Molecular pathogenesis of human cancer / William B. Coleman and Gregory J. Tsongalis -- Application of molecular diagnostics to hereditary nonpolyposis colorectal cancer / Ian M. Frayling ... [et al.] -- Molecular genetic applications to the diagnosis of lymphoma / William N. Rezuke, Jr. and Gregory J. Tsongalis -- Molecular genetic abnormalities in acute and chronic leukemias / Pei Lin and L. Jeffrey Medeiros -- Pt. VIII. Applications of molecular diagnostics for infectious diseases. Molecular testing for Chlamydia trachomatis and Neisseria gonorrhoeae / Alexandra Valsamakis -- Human Papillomavirus / Theresa M. Voytek and Gregory J. Tsongalis -- Molecular diagnosis for HIV-1 / Angela M. Caliendo and Charles E. Hill -- Hepatitis C / D. Robert Dufour -- Cytomegalovirus / Angela M. Caliendo and Nell S. Lurain -- Pt. IX. Applications of molecular diagnostics for identity-based testing. HLA typing using molecular methods / John L. Schmitz -- Molecular analysis for forensic casework and parentage testing / Tracey Dawson Cruz -- Molecular assessment of bone marrow transplant engraftment / Jessica K. Booker -- Use of DNA-based identity testing for specimen identification / Andrew Ricci Jr. and Gregory J. Tsongalis -- Pt. X. Issues for the clinical molecular pathology laboratory. Genetic counseling considerations in molecular diagnosis / Myra I. Roche -- Ethical, social, and legal issues related to molecular genetic testing / Jennifer L. Herbst and Jon F. Merz.
  • 2010 ScienceDirect
    [edited by] Wayne W. Grody ... [et al.].
    Advances in genomic and proteomic profiling of disease have transformed the field of molecular diagnostics, thus leading the way for a major revolution in clinical practice. While the range of tests for disease detection and staging is rapidly expanding, many physicians lack the knowledge required to determine which tests to order and how to interpret results. This handbook provides a complete guide to the use and interpretation of molecular testing in the clinical arena. No other available resource offers this emphasis, comprehensive scope, and practical utility in the clinical setting.
  • 2010 CRCnetBASE
    editors, Jan Trøst Jørgensen, Henrik Winther.
    This unique book provides a thorough overview of developing molecular cancer diagnostic assays, which are the prerequisites for optimal solutions within personalized cancer medicine. The book takes the reader through definitions of the pharmacodiagnostic concept, historical perspectives of the early steps into molecular cancer diagnostics linked to therapy, the basis of different diagnostic molecular techniques, ongoing research, drug-diagnostic co-development, assay validation, clinical trial methodology, regulatory issues around pharmacodiagnostics and future aspects within personalized cancer medicine.The book is the first of its kind to cover relevant subjects around molecular cancer diagnostics when used as important drivers in personalized cancer medicine. The different chapters of the book are authored by key opinion leaders, each representing their specialized areas, which provide the reader with expertise knowledge in the field.
  • 2010 ScienceDirect
    edited by George P. Patrinos, Wilhelm J. Ansorge.
    Chapter 1. Molecular Diagnostics: Past, Present, and Future / George P. Patrinos, Wilhelm J. Ansorge -- Chapter 2 . Allele-Specific Mutation Detection / Aglaia Athanassiadou, Eleana F. Stavrou, Adamandia Papachatzopoulou, George P. Patrinos -- Chapter 3. Enzymatic and Chemical Cleavage Methods to Identify Genetic Variation / Chinh T. Bui, Emmanuelle Nicolas, Georgina Sallmann, Maria Chiotis, Andreana Lambrinakos, Kylee Rees, Ian Trounce, Richard G.H. Cotton, Lauryn Blakesley, Andrew K. Godwin, Anthony T. Yeung -- Chapter 4. Mutation Detection by Single Strand Conformation Polymorphism and Heteroduplex Analysis / Panayiotis G. Menounos, George P. Patrinos -- Chapter 5. Capillary Electrophoresis / Anja Bosserhoff, Claus Hellerbrand -- Chapter 6. Temperature and Denaturing Gradient Gel Electrophoresis / Hartmut Peters, Peter N. Robinson -- Chapter 7. Real-Time Polymerase Chain Reaction / Lut Overbergh, Anna-Paula Giulietti, Dirk Valckx, Chantal Mathieu -- Chapter 8 - Pyrosequencing / Sharon Marsh -- Chapter 9. Application of Padlock and Selector Probes in Molecular Medicine / Mats Nilsson, Chatarina Larsson, Johan Stenberg, Jenny Göransson, Ida Grundberg, Magnus Isaksson, Tim Conze, Sara Henriksson -- Chapter 10. Molecular Cytogenetics in Molecular Diagnostics / Holger Tönnies -- Chapter 11. Analysis of Human Splicing Defects Using Hybrid Minigenes / Franco Pagani, Francisco E. Baralle -- Chapter 12. Detection of Genomic Duplications and Deletions / Graham R. Taylor, Carol A. Delaney -- Chapter 13. Multiplex Ligation-Dependent Probe Amplification (MLPA) and Methylation-Specific (MS)-MLPA: Multiplex Detection of DNA/mRNA Copy Number and Methylation Changes / Eline M. Sepers, Jan P. Schouten -- Chapter 14. Molecular Techniques for DNA Methylation Studies / Jörg Tost, Ivo G. Gut -- Chapter 15. High-Resolution Melting Curve Analysis for Molecular Diagnostics / Jared S. Farrar, Gudrun H. Reed, Carl T. Wittwer -- Chapter 16. DNA Microarrays and Genetic Testing / Lars Dyrskjøt, Karina Dalsgaard-Sørensen, Marie Stampe-Ostenfeld, Karin Birkenkamp-Demtroder, Kasper Thorsen, Claus L. Andersen, Mogens Kruhøffer, Jens L. Jensen, Torben F. Ørntoft -- Chapter 17. Arrayed Primer Extension Microarrays for Molecular Diagnostics / Neeme Tonisson, Eneli Oitmaa, Kaarel Krjutskov, Janne Pullat, Ilona Lind, Merike Leego, Ants Kurg, Andres Metspalu -- Chapter 18. Application of Proteomics to Disease Diagnostics / Samir Hanash, Ji Qiu, Vitor Faca -- Chapter 19. RNA-Based Variant Detection: The Protein Truncation Test / Johan T. den Dunnen -- Chapter 20. Protein Diagnostics by Proximity Ligation: Combining Multiple Recognition and DNA Amplification for Improved Protein Analyses / Karl-Johan Leuchowius, Ola Söderberg, Masood Kamali-Moghaddam, Malin Jarvius, Irene Weibrecht, Katerina Pardali, Ulf Landegren -- Chapter 21. Mass Spectrometry and its Applications to Functional Proteomics / Thomas Köcher, Giulio Superti-Furga -- Chapter 22. Pharmacogenetics and Pharmacogenomics: Impact on Drug Discovery and Clinical Care / Minoli Perera, Federico Innocenti -- Chapter 23. Nutrigenomics: Integrating Genomic Approaches into Nutrition Research / Lynette R. Ferguson, Martin Philpott, Matthew P.G. Barnett -- Chapter 24. Novel Next-Generation DNA Sequencing Techniques for Ultra High-Throughput Applications in Bio-Medicine / Wilhelm J. Ansorge -- Chapter 25. Locus-Specific and National/Ethnic Mutation Databases: Emerging Tools for Molecular Diagnostics / George P. Patrinos -- Chapter 26. Molecular Diagnostic Applications in Forensic Science / Bruce Budowle, John V. Planz, Rowan Campbell, Arthur J. Eisenberg -- Chapter 27. Mass Disaster Victim Identification Assisted by DNA Typing / Daniel Corach -- Chapter 28. Detection of Highly Pathogenic Viral Agents: Implications for Therapeutics, Vaccines and Biodefense / Kylene Kehn-Hall, Sina Bavari -- Chapter 29. Identification of Genetically Modified Organisms / Farid E. Ahmed -- Chapter 30. Molecular Diagnostics and Comparative Genomics in Clinical Microbiology / Alex van Belkum -- Chapter 31. Genetic Monitoring of Laboratory Rodents / Jean-Louis Guénet, Fernando J. Benavides -- Chapter 32. Safety Analysis in Retroviral Gene Therapy: Identifying Virus Integration Sites in Gene-Modified Cells / Stephanie Laufs, Luisa Schubert, Patrick Maier, W. Jens Zeller, Stefan Fruehauf -- Chapter 33. Preimplantation Genetic Diagnosis / Martine de Rycke, Karen Sermon -- Chapter 34. Automated DNA Hybridization and Detection / Bert Gold -- Chapter 35. The Use of Microelectronic-Based Techniques in Molecular Diagnostic Assays / Piotr Grodzinski, Michael Ward, Robin Liu, Kathryn Scott, Saul Surrey, Paolo Fortina -- Chapter 36. Human Gene Patents and Genetic Testing / Timothy Caulfield, Yann Joly -- Chapter 37. Genetic Counseling and Ethics in Molecular Diagnostics / Mary Petrou -- Chapter 38. Genetic Testing and Psychology / Andrea Farkas Patenaude -- Chapter 39. General Considerations Concerning Safety in Biomedical Research Laboratories / William E. Grizzle, Walter C. Bell, Jerry Fredenburgh -- Chapter 40. Quality Management in the Laboratory / David Burnett.
  • 2010 Springer
    Mousumi Debnath, Godavarthi B.K.S. Prasad, Prakash S. Bisen.
  • pt. [A]-B 1993-2005. ScienceDirect
    pt. B, 2005 ScienceDirect
    edited by Elizabeth A. Zimmer ... [et al.].
  • 2008 Springer
    edited by Liang Cheng, David Y. Zhang.
  • 2013 Springer
    Liang Cheng, David Y. Zhang, John N. Eble, editors.
    Molecular Genetic Pathology, Second Edition presents up-to-date material containing fundamental information relevant to the clinical practice of molecular genetic pathology. Fully updated in each area and expanded to include identification of new infectious agents (H1N1), new diagnostic biomarkers and biomarkers for targeted cancer therapy. This edition is also expanded to include the many new technologies that have become available in the past few years such as microarray (AmpliChip) and high throughput deep sequencing, which will certainly change the clinical practice of molecular genetic pathology. Part I examines the clinical aspects of molecular biology and technology, genomics. Poharmacogenomics and proteomics, while Part II covers the clinically relevant information of medical genetics, hematology, transfusion medicine, oncology, and forensic pathology. Supplemented with many useful figures and presented in a helpful bullet-point format, Molecular Genetic Pathology, Second Edition provides a unique reference for practicing pathologists, oncologists, internists, and medical genetisists. Furthermore, a book with concise overview of the field and highlights of clinical applications will certainly help those trainees, including pathology residents, genetics residents, molecular pathology fellows, internists, hematology/oncology fellows, and medical technologists in preparing for their board examination/certification.
  • 1987 ScienceDirect
    edited by Michael M. Gottesman.
    Also available: Print – 1987
  • 2013 Springer
    Diane M. Simeone, Anirban Maitra, editors.
    Genomic Alterations in Sporadic Pancreatic Cancer / Marco Dal Molin, Anirban Maitra -- Molecular Pathology of Pancreatic Cancer Precursor Lesions / Lodewijk A. A. Brosens, G. Johan Offerhaus -- Genetic Epidemiology and Pancreatic Cancer / Li Jiao, Donghui Li -- Translational Implications of Molecular Genetics for Early Diagnosis of Pancreatic Cancer / Michael A. Hollingsworth -- The Biology of K-Ras Signaling Pathways in Pancreatic Cancer / Helen Court, Mark R. Philips, Dafna Bar-Sagi -- Molecular Targeted Therapies in Pancreatic Cancer / Edward Kim, Ethan V. Abel, Arunima Ghosh -- Mouse Models of Pancreatic Ductal Adenocarcinoma / Filip Bednar, Marina Pasca di Magliano -- The Genetics of Pancreatic Cancer Progression / Christine A. Iacobuzio-Donahue, -- Epigenetic Alterations in Pancreatic Cancer / Michael Ayars, Michael Goggins -- Innovative Technologies in the Molecular Characterization of Pancreatic Cancer / Iris H. Wei, Chandan Kumar-Sinha.
  • 2007 Springer
    Andrés Aguilera, Rodney Rothstein, (eds.).
    Also available: Print – 2007
  • NCBI Bookshelf
    This searchable database is a collection of in vivo molecular imaging agents developed for positron emission tomography (PET), single photon emission computed tomography (SPECT), magnetic resonance imaging (MRI), ultrasound (US), computed tomography (CT), and optical imaging. MICAD, a key component of the "Molecular Libraries and Imaging" program of the NIH Roadmap, is being developed by NCBI, the National Center for Biotechnology Information".
  • 2015
    S.S. Gambhir.
    Status: Not Checked OutLane Catalog Record
    Chapter 1. Molecular imaging -- Chapter 2. Basic cell & molecular biology -- Chapter 3. Ovreview of imaging modalities -- Chapter. 4. Molecular imaging strategies -- Chapter 5. Assays -- Chapter 6. Nanoparticle imaging -- Chapter 7. Clinical applications -- Chapter 8. Molecular imaging examples -- Chapter 10. Frequently asked questions -- Chapter 11. Useful tables -- Chapter 12. Probe structures table.
  • 2011 Springer
    Albert M. Wu, editor.
    Part 1. Introduction to Glyco-Experiences -- Serendipity in Scientific Discoveries: Some Examples in Glycosciences / Yuan-Chuan Lee -- My 30-Year Devotion to the N-Linked Oligosaccharide Structures / Noriko Takahashi -- Part 2. Blood Group ABH/Le-related Antigens -- Human Blood Group ABH/Ii, Lea,b,x,y, and Sialyl Lea,x Glycotopes; Internal Structures; and Immunochemical Roles of Human Ovarian Cyst Glycoproteins / Albert M. Wu -- Lewis Glyco-Epitopes: Structure, Biosynthesis, and Functions / Hui-Li Chen -- A Unique Endo-β-Galactosidase that Cleaves Both Blood Group A and B Glycotopes / Su-Chen Li, Kimberly M. Anderson and Yu-Teh Li -- Part 3. Lectins -- Recognition Roles of Mammalian Structural Units and Polyvalency in Lectin-Glycan Interactions / Albert M. Wu, Jia-Haw Liu, Tanuja Singh and Zhangung Yang -- Adhesion/Growth-Regulatory Galectins: Insights into Their Ligand Selectivity Using Natural Glycoproteins and Glycotopes / Albert M. Wu, Tanuja Singh, Jia-Hau Liu, Sabine André and Martin Lensch, et al. -- Glycotope Structures and Intramolecular Affinity Factors of Plant Lectins for Tn/T Antigens / Pierre Rougé, Willy J. Peumans, Els J. M. Van Damme, Annick Barre and Tanuja Singh, et al. -- The Five Bacterial Lectins (PA-IL, PA-IIL, RSL, RS-IIL, and CV-IIL): Interactions with Diverse Animal Cells and Glycoproteins / Nechama Gilboa-Garber, Keren D. Zinger-Yosovich, Dvora Sudakevitz, Batya Lerrer and Anne Imberty, et al. -- On the Differential Sialic Acid Specificity of Lectins from Different Parts of Saraca indica / Bishnu P. Chatterjee and Mainak Majumder -- Regulation of Lectin Production by the Human Pathogens Pseudomonas aeruginosa and Chromobacterium violaceum: Effects of Choline, Trehalose, and Ethanol / Nachman C. Garber, Keren D. Zinger-Yosovich, Dvora Sudakevitz, Itschak Axelrad and Nechama Gilboa-Garber -- Non-carbohydrate-Mediated Interaction of Lectins with Plant Proteins / Jared Q. Gerlach, Michelle Kilcoyne, Seron Eaton, Veer Bhavanandan and Lokesh Joshi -- Novel Concepts About the Role of Lectins in the Plant Cell / Els J. M. Van Dammes, Elke Fouquaert, Nausicaä Lannoo, Gianni Vandenborre and Dieter Schouppe, et al. -- Part 4. Structures and Functions of Glycolipids -- Role of Gangliosides and Plasma Membrane-Associated Sialidase in the Process of Cell Membrane Organization / Sandro Sonnino, Vanna Chigorno, Massimo Aureli, Anie Priscilla Masilamani and Manuela Valsecchi, et al. -- 9-O-Acetyl GD3 in Lymphoid and Erythroid Cells / Kankana Mukherjee, Suchandra Chowdhury, Susmita Mondal, Chandan Mandal and Sarmila Chandra, et al. -- GM3 Upregulation of Matrix Metalloproteinase-9 Possibly Through PI3K, AKT, RICTOR, RHOGDI-2, and TNF-A Pathways in Mouse Melanoma B16 Cells / Pu Wang, Xiaodong Wang, Peixing Wu, Jinghai Zhang and Toshinori Sato, et al. -- Pathological Roles of Ganglioside Mimicry in Guillain-Barré Syndrome and Related Neuropathies / Robert K. Yu, Toshio Ariga, Seigo Usuki and Ken-ichi Kaida -- Structure and Function of Glycolipids in Thermophilic Bacteria / Feng-Ling Yang, Yu-Liang Yang and Shih-Hsiung Wu -- Lipooligosaccharides of Neisseria Species: Similarity Between N. polysaccharea and N. meningitidis LOSs / Chao-Ming Tsai -- Part 5. Structures and Functions of Complex Carbohydrates -- Roles for N- and O-Glycans in Early Mouse Development / Suzannah A. Williams and Pamela Stanley -- Glycobiology in the Field of Gerontology (Glycogerontology) / Akira Kobata -- Galectins in Regulation of Apoptosis / Fu-Tong Liu, Ri-Yao Yang, Jun Saegusa, Huan-Yuan Chen and Daniel K. Hsu -- Avian and Human Influenza Virus Receptors and Their Distribution / Yasuo Suzuki -- Importance of a Factor VIIIc-Like Glycoprotein Expressed in Capillary Endothelial Cells (eFactor VIIIc) in Angiogenesis / Dipak K. Banerjee, Caroline M. Oliveira, José J. Tavárez, Viswa N. Katiyar and Subiman Saha, et al. -- Mucin O-Glycan Branching Enzymes: Structure, Function, and Gene Regulation / Pi-Wan Cheng and Prakash Radhakrishnan -- Studying Carbohydrate Self-Recognition in Marine Sponges Using Synthetic Aggregation Factor Epitopes / Johannis P. Kamerling and Adriana Carvalho de Souza -- Docking of Chitin Oligomers and Nod Factors on Lectin Domains of the LysM-RLK Receptors in the Medicago-Rhizobium Symbiosis / Pierre Rougé, Wim Nerinckx, Clare Gough, Jean-Jacques Bono and Annick Barre -- Part 6. Glycoimmunology -- O-Acetylated Sialic Acids and Their Role in Immune Defense / Roland Schauer, G. Vinayaga Srinivasan, Dirk Wipfler, Bernhard Kniep and Reinhard Schwartz-Albiez -- Sialylated and Sulfated Carbohydrate Ligands for Selectins and Siglecs: Involvement in Traffic and Homing of Human Memory T and B Lymphocytes / Reiji Kannagi, Katsuyuki Ohmori, Guo-Yun Chen, Keiko Miyazaki and Mineko Izawa, et al. -- Diversity of Natural Anti-α-Galactosyl Antibodies in Human Serum / Elwira Lisowska and Maria Duk -- Significance of Serum Glycoprotein Profiles in Spontaneous Tolerance After Liver Allograft Transplantation / Pei-Weng Wang and Tai-Long Pan -- Part 7. Glycobiology of Cancer -- Hematogenous Metastasis: Roles of CD44v and Alternative Sialofucosylated Selectin Ligands / Konstantinos Konstantopoulos and Susan N. Thomas -- Regulation of Glycosyltransferase Genes in Apoptotic Breast Cancer Cells Induced by l-PPMP and Cisplatin / Rui Ma, Elizabeth A. Hopp, N. Matthew Decker, Audrey Loucks and James R. Johnson, et al. -- Aberrant Glycosphingolipid Expression and Membrane Organization in Tumor Cells: Consequences on Tumor-Host Interactions / Alessandro Prinetti, Simona Prioni, Nicoletta Loberto, Massimo Aureli and Valentina Nocco, et al. -- Human KDN (Deaminated Neuraminic Acid) and Its Elevated Expression in Cancer Cells: Mechanism and Significance / Sadako Inoue, Ken Kitajima, Chihiro Sato and Shinji Go -- Polysialic Acid Bioengineering of Cancer and Neuronal Cells by N-Acyl Sialic Acid Precursor Treatment / Robert A. Pon, Wei Zou and Harold J. Jennings -- Part 8. Glyco Applications -- Synthesis of Hemagglutinin-Binding Trisaccharides / Cheng-Chung Wang, Suvarn S. Kulkarni, Medel Manuel L. Zulueta and Shang-Cheng Hung -- Fabrication and Applications of Glyconanomaterials / Po-Chiao Lin, Avijit Kumar Adak and Chun-Cheng Lin -- Glycan Arrays to Decipher the Specificity of Plant Lectins / Els J. M. Van Damme, David F. Smith, Richard Cummings and Willy J. Peumans -- Targeting C-Type Lectin for the Treatment of Flavivirus Infections / Szu-Ting Chen, Yi-Ling Lin, Ming-Ting Huang, Ming-Fang Wu and Shie-Liang Hsieh.
    Also available: Print – 2011
  • 2010 Karger
    volume editor, Karl Lenhard Rudolph.
    Speakers at the Symposium / Hartmann, D. -- Hematopoietic stem cell aging and fate decision / Illing, A., Ju, Z. -- Alterations of systemic environment impair stem cell function during aging / Song, Z., Ju, Z. -- Novel insights into regulation of stem cell aging by aging of the niche and alterations in systemic factors / Geiger, H. -- Aging and hematopoietic malignancies / Gompf, A. -- Intestinal stem cell aging / Sperka, T., Rudolph, K.L. -- Molecular mechanisms of aging in muscle stem cells / Tümpel, S. -- Free radicals and stem cell aging / Guachalla, L.M. -- DNA damage, checkpoint responses, and cell cycle control in aging stem cells / Sperka, T., Nalapareddy, K., Rudolph, K.L.
  • 2007 Springer
    Georg F. Weber.
    Theories of carcinogenesis -- Physioloigic correlates of malignancy -- Cell division and survival -- Cellular senescence -- Invasiveness -- DNA repair -- Epigenetic regulation of cancer-associated gene products -- Interaction of the groups of cancer-related gene products -- Structural growth control -- The organ preference of metastasis formation -- Angiogenesis -- Interactions with the immune system -- Endocrine dysregulation -- Dormancy and minimal residual disease -- Epithelial tumors -- Hematologic malignancies -- Mesenchymal tumors -- Neurologic tumors -- Tumors of serous cavities -- Embryonic tumors.
  • 2002 ScienceDirect
    edited by Max Sussman.
  • 2003 ScienceDirect
    edited by J. Joseph Marr, Timothy W. Nilsen, Richard W. Komuniecki.
    Sect. I. Molecular Biology -- Ch. 1. Parasite genomics -- Ch. 2. RNA processing in parasitic organisms: trans-splicing and RNA editing -- Ch. 3. Transcription -- Ch. 4. Post-transcriptional regulation -- Ch. 5. Antigenic variation in African trypanosomes and malaria -- Ch. 6. Genetic and genomic approaches to the analysis of leishmania virulence -- Sect. II. Biochemistry and Cell Biology: Protozoa -- Ch. 7. Energy metabolism -- Ch. 8. Amino acid and protein metabolism -- Ch. 9. Purine and pyrimidine transport and metabolism -- Ch. 10. Trypanosomatid surface and secreted carbohydrates -- Ch. 11. Intracellular signaling -- Ch. 12. Plastids, mitochondria, and hydrogenosomes -- Sect. III. Biochemistry and Cell Biology: Helminths -- Ch. 13. Helminth surfaces: structural, molecular and functional properties -- Ch. 14. Carbohydrate and energy metabolism in parasitic helminths -- Ch. 15. Neurotransmitters -- Sect. IV. Medical Applications -- Ch. 16. Drug resistance in parasites -- Ch. 17. Medical implications of molecular parasitology.
  • 2012 ScienceDirect
    2012 ClinicalKey
    Ronald J. Trent.
    Molecular Medicine is the application of genetic or DNA-based knowledge to the modern practice of medicine. Molecular Medicine, 4e, provides contemporary insights into how the genetic revolution is influencing medical thinking and practice. The new edition includes recent changes in personalized medicine, new growth in omics and direct-to-consumer DNA testing, while focusing on advances in the Human Genome project and implications of the advances in clinical medicine. Graduate students, researchers, clinicians and allied health professionals will appreciate the background history and clinical application of up-to-date molecular advances. Extensively revised to incorporate the results of the Human Genome Project, it provides the latest developments in molecular medicineThe only book in Molecular Medicine to reach its fourth edition Identifies current practice as well as future developmentsPresents extensive tables, well presented figures and resources for further understanding.
  • 2011 Springer Protocols
    edited by Virginie Orgogozo, Matthew V. Rockman.
    Characterizing the genome. Genome size determination using flow cytometry of propidium iodide-stained nuclei / Emily E. Hare and J. Spencer Johnston. Chromosome analysis in invertebrates and vertebrates / David M. Rowell, Shu Ly Lim, and Frank Grutzner. Genomic libraries : I. Constuction and screening of fosmid genomic libraries / Mike A. Quail [and others] Genomic libraries : II. Subcloning, sequencing, and assembling large-insert genomic DNA clones / Mike A. Quail [and others] -- Targeting regions of the genome. Reduced representation methods for subgenomic enrichment and next-generation sequencing / Jeffrey M. Good. Accessing the transcriptome : how to normalize mRNA pools / Heiko Vogel and Christopher W. Wheat. Transcriptome sequencing goals, assembly, and assessment / Christopher W. Wheat and Heiko Vogel. Rapid retrieval of DNA target sequences by primer extension capture / Adrian W. Briggs -- Measuring genetic diversity. SNP discovery and genotyping for evolutionary genetics using RAD sequencing / Paul D. Etter [and others] DNA microarray-based mutation discovery and genotyping / David Gresham. Genotyping with Sequenom / Martina Bradić, João Costa, and Ivo M. Chelo. Isolating microsatellite loci : looking back, looking ahead / José A. Andrés and Steven M. Bogdanowicz. Design of custom oligonucleotide microarrays for single species or interspecies hybrids using array olico selector / Amy A. Caudy -- Obtaining candidate gene sequences. Identification of homologous gene sequences by PCR with degenerate primers / Michael Lang and Virginie Orgogozo -- Characterizing cDNA ends by circular RACE / Patrick T. McGrath. Identification of DNA sequences that flank a known region by inverse PCR / Anastasios Pavlopoulos -- Analyzing candidate gene transcripts. Quantification of transcript levels with quantitative RT-PCR / Karen L. Carleton. Using pyrosequencing to measure allele-specific mRNA abundance and infer the effects of cis- and trans-regulatory differences / Patricia J. Wittkopp. Whole-mount in situ hybridization of sectioned tissues of species hybrids to detect cis-regulatory changes in gene expression pattern / Ryo Futahashi. Identifying fluorescently labeled single molecules in image stacks using machine learning / Scott A. Rifkin -- Testing candidate genes and candidate mutations. Experimental approaches to evaluate the contributions of candidate cis-regulatory mutations to phenotypic evolution / Mark Rebeiz and Thomas M. Williams. Experimental approaches to evaluate the contributions of candidate protein-coding mutations to pheotypic evolution / Jay F. Storz and Anthony J. Zera. Making reporter gene constructs to analyze cis-regulatory elements / José Bessa and José Luis Gómez-Skarmeta. PCR directed in vivo plasmid construction using homologous recombination in baker's yeast / Erik C. Andersen. Production of fosmid genomic libraries optimized for liquid culture recombineering and cross-species transgenesis / Radoslaw Kamil Ejsmont [and others] Recombination-mediated genetic engineering of large genomic DNA transgenes / Radoslaw Kamil Ejsmont [and others] Overlap extension PCR : an efficient method for transgene construction / Matthew D. Nelson and David H.A. Fitch. Gene knockdown analysis by double-stranded RNA injection / Benjamin N. Philip and Yoshinori Tomoyasu.
  • 2015 Springer Protocols
    edited by Andreas Kukol, University of Hertfordshire, Hatfield, Hertfordshire, UK.
    Molecular dynamics simulations / Erik R. Lindahl -- Monte Carlo simulations / David J. Earl and Michael W. Deem -- Hybrid quantum and classical methods for computing kinetic isotope effects of chemical reactions in solutions and in enzymes / Jiali Gao ... [et al.] -- Comparison of protein force fields for molecular dynamics simulations / Olgun Guvench and Alexander D. MacKerell, Jr. -- Normal modes and essential dynamics / Steven Hayward and Bert L. de Groot -- Calculation of absolute protein-ligand binding constants with the molecular dynamics free energy perturbation method / Hyung-June Woo -- Free energy calculations applied to membrane proteins / Christophe Chipot -- Molecular dynamics simulations of membrane proteins / Philip C. Biggin and Peter J. Bond -- Membrane-associated proteins and peptides / Marc F. Lensink -- Implicit membrane models for membrane protein simulation / Michael Feig -- Comparative modeling of proteins / Gerald H. Lushington -- Transmembrane protein models based on high-throughput molecular dynamics simulations with experimental constratints / Andrew J. Beevers and Andreas Kukol -- Nuclear magnetic resonance-based modeling and refinement of protein three-dimensional structures and their complexes / Gloria Fuentes, Aalt D.J. van Dijk, and Alexandre M.J.J. Bonvin -- Conformational changes in protein function / Haiguang Liu ... [et al.] -- Protein folding and unfolding by all-atom molecular dynamics simulations / Hongxin Lei and Yong Duan -- Modeling of protein misfolding in disease / Edyta B. Małolepsza -- Identifying putative drug targets and potential drug leads : starting points for virtual screening and docking / David S. Wishart -- Receptor flexibility for large-scale in silico ligand screens : chances and challenges / B. Fischer, H. Merlitz, and W. Wenzel -- Molecular docking / Garrett M. Morris and Marguerita Lim-Wilby.
  • 2015 Springer
    Michael Ola Idowu, Catherine Isabelle Dumur, Carleton Theodore Garrett, editors.
    Part I. Overview of Basic Cellular Biology -- 1. Normal molecular biology and definition of terms -- 2. Pathologic changes: Chromosomes, genes, DNA, RNA and proteins -- 3. Standard nomenclature -- introduction and brief overview -- Part II. Molecular Oncology Methodology -- 4. General principles of Oncology testing -- 5. Molecular methodologies -- 6. General principles of validating a molecular test -- Part III. Molecular testing in Solid Tumors -- 7. Lung and mediastinum -- 8. Breast -- 9. Head and neck (salivary glands) -- 10. Thyroid and parathyroid (endocrine) -- 11. Gastrointestinal and Pancreatobiliary -- 12. Soft tissue -- 13. Genitourinary system -- 14. Central Nervous System -- 15. Skin and adnexa -- 16. Common Hereditary Cancer Syndromes -- Part IV. The future is here -- 17. Application of next generation sequencing -- Part V. Quality Assurance and Regulatory affairs -- 18. Quality Assurance -- pre-analytic, analytic and post-analytic -- 19. Regulatory affairs (including accreditation) -- 20. Coding and re-imbursement.
  • 2006 Springer
    Cheryl A. Nickerson, Michael J. Schurr, editors.
    Genetic analysis of bacterial pathogenesis -- Genetic exchance in bacteria and the modular stucture of mobile DNA elements -- Genomics and the use of genomic tools to study pathogenic bacteria -- Pathogenicity islands and bacterial virulence -- Capsules -- Bacterial cell walls -- Mechanisms of bacterial adhesion and consequences of attachment -- Bacterial invasion into non-phagocytic cells -- Bacterial protein secretion mechanisms -- Toxins as host cell modulators -- Quorum sensing: coordinating group behavior through intercellular signals -- The role of sigma factors in regulating bacterial stress responses and pathogenesis -- Two-component regulatory systems -- Oxidative stress systems in bacteria: four model organisms -- Bacterial biowarfare agents.
    Also available: Print – 2006
  • 2015 Cambridge
    edited by John M.S. Bartlett, Ontario Institute for Cancer Research, Toronto, Ontario, Canada, Abeer Shaaban, Queen Elizabeth Hospital, Birmingham, UK and Tanta University Hospital, Tanta, Egypt, Fernando Schmitt, The Laboratoire National de Santé, Dudelange, Luxembourg.
    1. An introduction to molecular pathology / John M.S. Bartlett -- 2. Molecular regulation of cellular function / Marsela Braunstein and John M.S. Bartlett -- 3. Practical application of molecular techniques in diagnostic histopathology and cytopathology and clinical management / Abeer Shaaban and Fernando Schmitt -- 4. Fluorescent and non-fluorescent in situ hybridization / Jane Bayani and John M.S. Bartlett -- 5. Clinical applications of the polymerase chain reaction for molecular pathology / Angela B.Y. Hui, Annette D. Wong and Carlo V. Hojilla -- 6. Are microarrays ready for prime time? / Jane Bayani and John M.S. Bartlett -- 7. Tissue microarrays / Luigi Tornillo -- 8. Sequencing / Andrew M.K. Brown, Philipe C. Zuzarte and Paul M. Krzyzanowski -- 9. Microsatellite instability in colorectal cancer / Maria Sofia Fernandes, Raquel Seruca and Se;rgia Velho -- 10. Mutational analysis / Aaron R. Hansen, Mahadeo A. Sukhai, Hal K. Berman, Suzanne Kamel-Reid and Philippe L. Bedard -- 11. Tumors of the breast / Cathy B. Moelans, Petra van der Groep, Robert Kornegoor, Jelle Wesseling and Paul J. van Diest -- 12. Tumors of the female genital system / Adhemar Longatto Filho, Sigurd F. Lax and Bin Yang -- 13. Tumours of the male urogenital system / Kirsten D. Mertz and Lukas Bubendorf -- 14. Tumors of the gastrointestinal system / Manuel Salto-Tellez, Benedict Yan, Roseann I. Wu and Martha Bishop Pitman; 15. Soft tissue, bone and skin tumours Stamatios Theocharis and Jerzy Klijanienko; 16. Cardio-thoracic tumors Umberto Malapelle, Claudio Bellevicine, Elena Vigliar and Giancarlo Troncone; 17. Tumors of the endocrine system Sylvia L. Asa and Ozgur Mete -- 18. Haematological malignancies of the lymph nodes / Sarah E. Coupland and Pedro Farinha -- 19. Pediatric tumors / Paul Roberts, Susan Picton and Jens Stahlschmidt -- 20. Serous effusions / Ben Davidson -- 21. Academic applications and future of molecular pathology / Manuel Salto-Tellez and Benedict Yan -- Index.
  • 2016 Springer
    Debra G.B. Leonard, editor.
    I. Genetics -- II. Inherited Cancers -- III. Solid Tumors -- IV. Neoplastic Hematopathology -- V. Infectious Diseases -- VI. Identity Testing -- VII. HLA Typing -- VIII. Evolving Clinical Molecular Technologies -- IX. Laboratory Management.-Index.
  • 2007 Springer
    editor, Debra G.B. Leonard ; section editors: Adam Bagg ... [et al.].
    Basics of molecular biology -- Molecular pathology methods -- Genetic counseling -- Pedigree analysis and risk assessment -- Bayesian analysis -- Developmental disabilities -- Neuromuscular diseases -- Molecular genetic testing for metabolic disorders -- Fibroblast growth factor receptor-related skeletal disorders -- Cystic fibrosis -- Deafness -- Coagulation disorders -- Hematologic disorders : hemochromatosis, hemoglobinopathies, and Rh incompatibility -- Cardiovascular disease -- Neurodegenerative disorders -- Skin and connective tissue disorders -- Inherited breast cancer -- Familial adenomatous polyposis and Turcot and Peutz-Jeghers syndromes -- Hereditary nonpolyposis colorectal cancer -- Multiple endocrine neoplasia syndromes -- The neurofibromatoses -- TP53 mutation and Li-Fraumeni syndrome -- von Hippel-Lindau disease -- Breast cancer -- Urothelial carcinoma -- Sarcomas -- Gliomas -- Molecular detection of occult tumor cells -- Microarray studies (Beyond histology) -- Acute myeloid leukemia -- Acute lymphoblastic leukemia -- B-cell lymphomas -- T-cell lymphomas -- Lymphoproliferations of immunodeficiency -- Myeloproliferative disorders and myelodysplastic syndromes -- Human immunodeficiency virus type 1 -- Hepatitis B and C viruses -- Viral infections in transplant patients -- Viral infections of the central nervous system -- Sexually transmitted diseases -- Respiratory pathogens -- Bacterial pathogens -- Mycobacterial infections -- Forensic DNA typing -- Parentage and relationship testing -- Assessment of chimerism in the setting of allogenic hematopoietic cell transplantation -- Specimen identification through DNA analysis -- Molecular HLA typing -- Molecular pathology laboratory management -- From research to clinical practice.
  • v. 1-, 2008- Springer
  • 2013 Springer
    Lu-qi Huang, editor.
    "This book reviews the latest developments in pharmacognosy, introduces a series of new views and insights, presents the hotspots and focus of the field of study on molecular pharmacognosy, and predicts a new direction of study on the resource science of TCM. Furthermore, the book also provides an open communications platform for the development of molecular pharmacognosy."--Publisher.
  • 2014 Springer Protocols
    edited by Pascale Besse.
    Plant Taxonomy: An Historical Perspective, Current Challenges and Perspectives -- Guidelines for the Choice of Sequences for Molecular Plant Taxonomy -- Leaf Tissue Sampling and DNA Extraction Protocols -- DNA Extraction from Herbarium Specimens -- Analysis of Variation in Chloroplast DNA Sequences -- Mitochondrial Genome and Plant Taxonomy -- Nuclear Ribosomal RNA Genes: ITS Region -- New Technologies for Ultra-High Throughput Genotyping in Plant Taxonomy -- Development of Microsatellite Enriched Libraries -- Randomly Amplified Polymorphic DNA (RAPD) and Derived Techniques -- Multilocus Profiling with AFLP, ISSR and SAMPL -- Transposon Based Tagging: IRAP, REMAP and iPBS -- Phylogenetic Reconstruction Methods: An Overview -- The Application of Flow Cytometry for Estimating Genome Size and Ploidy Level in Plants -- Molecular Cytogenetics (FISH and Flurochrome Banding): Resolving Species Relationships and Genome Organisation -- GISH: Resolving Interspecific and Intergeneric Hybrids -- On the Relevance of Molecular Tools for the Taxonomy Revision in Malvales, Malvaceae s.l. and Dombeyoideae -- What Has Molecular Systematics Contributed to Our Knowledge of the Plant Family Proteaceae.
  • 2012 Springer Protocols
    edited by Virginia Espina and Lance A. Liotta.
    Tumor staging and grading: A primer -- Clinical trial design in the age of molecular profiling -- Personalized medicine: ethics for clinical trials -- Reduction of preanalytical variability in specimen procurement for molecular profiling -- The human side of cancer biobanking -- Introduction to genomics -- Genomic profiling: cDNA arrays and oligoarrays -- Genome-wide methylation profiling in archival formalin-fixed paraffin-embedded tissue samples -- An overview of microRNA methods: expression profiling and target identification -- Antibody validation by western blotting -- Laser capture microdissection: Arcturus(XT) infrared capture and UV cutting methods -- Antibody microarrays: Analysis of cystic fibrosis -- Tissue microarrays as a tool in the discovery and validation of predictive biomarkers -- Reverse-phase protein microarrays -- Serum low-molecular-weight protein fractionation for biomarker discovery -- Mass spectrometry-based biomarker discovery -- Mitochondrial proteome: toward the detection and profiling of disease associated alterations -- Adult neural stem cells: Isolation and propagation -- Evanescent-wave field imaging: An introduction to total internal reflection fluorescence microscopy -- Construction and hyperspectral imaging of quantum dot lysate arrays -- Microarray data analysis: Comparing two population means -- Bioinformatics/biostatistics: microarray analysis -- Structure-based functional design of drugs: From target to lead compound -- Personalized medicine: Changing the paradigm of drug development -- Grant writing tips for translational research -- Inventions and patents: a practical tutorial -- Regulatory approval pathways for molecular diagnostic technology -- Small business development for molecular diagnostics.
  • 2011 ScienceDirect
    Betsy Foxman.
    The application of modern molecular genetic and biologic techniques to infectious disease epidemiology dramatically improves measurement of disease and putative risk factors increasing our ability to detect and track outbreaks, identify risk factors and detect new infectious agents. However, integration of these techniques into epidemiologic studies also poses new challenges in the design, conduct, and analysis. We examine these opportunities and methodologic challenges giving specific examples. The book will be written for the reader with limited understanding of genetics, biology and epidemiology. *Presents the key points of consideration when integrating molecular biology and epidemiology *Discusses how using molecular tools in epidemiologic research affects program design and conduct *Considers the ethical concerns that arise in molecular epidemiologic studies *Provides a context for understanding and interpreting scientific literature as a foundation for subsequent practical experience in the laboratory and in the field.
  • 2006
    P. David Josephy, Bengt Mannervik.
    Status: Not Checked OutLane Catalog Record
  • 2014 Springer Protocols
    edited by Phouthone Keohavong, Stephen G. Grant.
    Array-Based Immunoassays with Rolling-Circle Amplification Detection -- Analysis of Protein Changes Using Two-Dimensional Difference Gel Electrophoresis -- Assessment of Pathological and Physiological Changes in Mouse Lung Through Bronchoalveolar Lavage -- Analysis of Clinical and Biological Samples Using Microsphere-Based Multiplexing Luminex System -- Detection of DNA Methylation by MeDIP and MBDCap Assays: An Overview of Techniques -- Screening of DNA Methylation Changes by Methylation-Sensitive Random Amplified Polymorphic DNA-Polymerase Chain Reaction (MS-RAPD-PCR) -- Strategies for Measurement of Biotransformation Enzyme Gene Expression -- Genotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism Screening -- TaqMan Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material -- 32P-Postlabeling Analysis of DNA Adducts -- Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot -- DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry -- Analysis of DNA Strand Cleavage at Abasic Sites -- Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes Without Mitogen Stimulation for Chromosome Aberration Analysis Using Specific Whole Chromosome DNA Hybridization Probes -- Mutagen Sensitivity as Measured by Induced Chromatid Breakage as a Marker of Cancer Risk -- Pulsed-Field Gel Electrophoresis Analysis of Multicellular DNA Double-Strand Break Damage and Repair -- Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice -- The Blood-Based Glycophorin A (GPA) Human In Vivo Somatic Mutation Assay -- Flow Cytometric Quantification of Mutant T Cells with Altered Expression of the T-Cell Receptor: Detecting Somatic Mutants in Humans and Mice -- Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes. (Continued) -- Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems -- The Human T-Cell Cloning Assay: Identifying Genotypes Susceptible to Drug Toxicity and Somatic Mutation -- Molecular Analysis of Mutations in the Human HPRT Gene -- Simultaneous Quantification of t(14; 18) and HPRT Exon 2/3 Deletions in Human Lymphocytes -- Mutation Screening of the TP53 Gene by Temporal Temperature Gel Electrophoresis (TTGE) -- Detection of Point Mutations of K-ras Oncogene and p53 Tumor-Suppressor Gene in Sputum Samples -- ACB-PCR Quantification of Somatic Oncomutation -- Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection: Limitations and Solutions -- Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions -- Quantitative PCR-Based Measurement of Nuclear and Mitochondrial DNA Damage and Repair in Mammalian Cells -- The Sister Chromatid Exchange (SCE) Assay -- The Gene Cluster Instability (GCI) Assay for Recombination -- Measuring Recombination Proficiency in Mouse Embryonic Stem Cells -- Microsatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair Machinery -- Unscheduled DNA Synthesis: The Clinical and Functional Assay for Global Genomic DNA Nucleotide Excision Repair -- Analysis of Actively Transcribed DNA Repair Using a Transfection-Based System -- An Immunoassay for Measuring Repair of UV Photoproducts -- Analysis of Double-Strand Break Repair by Nonhomologous DNA End Joining in Cell-Free Extracts from Mammalian Cells -- Bioenergetic Analysis of Intact Mammalian Cells Using the Seahorse XF24 Extracellular Flux Analyzer and a Luciferase ATP Assay -- Quantification of Selective Phosphatidylserine Oxidation During Apoptosis -- Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance (EPR) Spectroscopy and Annexin-Conjugated Iron -- Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay.
  • 2013 Springer
    Ivano de Filippis, Marian L. McKee, editors.
    Part 1. General consideration on microorganism typing methods -- Molecular Epidemiology / Amy E. Seitz and D. Rebecca Prevots -- Part 2. Gastrointestinal Pathogens -- Enterococcus / Lúcia Martins Teixeira and Vânia Lúcia Carreira Merquior -- Listeria monocytogenes / Todd J. Ward -- Enterobacteriaceae / Steven L. Foley, Aaron M. Lynne and Rajesh Nayak -- Molecular Typing of Vibrio cholerae: Imprints in the Epidemiology of Cholera / T. Ramamurthy, A. K. Mukhopadhyay, R. K. Nandy and G. Balakrish Nair -- Molecular Typing of Clostridium difficile / Andrej Weintraub and Carl Erik Nord -- Oral and Intestinal Bacteroidetes / Marina C. Claros and Georg Conrads -- Part 3. Oral and Respiratory Pathogens -- Streptococci / Lesley McGee and Bernard Beall -- Molecular Typing of Streptococcus mutans / Kazuhiko Nakano, Ichiro Nakagawa, Satu Alaluusua and Takashi Ooshima -- Genotyping of Periodontal Anaerobic Bacteria in Relationship to Pathogenesis / Masae Kuboniwa and Atsuo Amano -- Molecular Typing of Nontuberculous Mycobacteria / Jakko van Ingen and Dick van Soolingen -- Molecular Typing of Neisseria meningitidis / Muhamed-Kheir Taha and Ala-Eddine Deghmane -- Molecular Typing in Bacterial Infections Haemophilus spp. / Marina Cerquetti -- Moraxella / Nevada M. Pingault and Thomas V. Riley -- Legionella pneumophila Typing / Christophe Ginevra -- Mycoplasma and Ureaplasma / Ken B. Waites, Li Xiao, Vanya Paralanov, Rose M. Viscardi and John I. Glass -- Corynebacterium diphtheriae / Igor Mokrousov -- Burkholderia / Pavel Drevinek and Eshwar Mahenthiralingam -- Part 4. Urogenital Pathogens -- Treponema / Allan Pillay -- Chlamydiaceae / Troy Skwor and Deborah Dean -- Part 5. Vector borne Pathogens -- Borreliae / Dionysios Liveris, Klára Hanincová and Ira Schwartz -- Erysipelothrix / Yumiko Imada -- Part 6. Pathogens causing Healthcare-Associated Infection -- Staphylococci / Jodi A. Lindsay -- Molecular Typing Methods for the Genus Pseudomonas / Balázs Libisch -- Part 7. Emerging and Reemerging Pathogens -- Acinetobacter baumannii / Lenie Dijkshoorn -- Molecular Typing of Coxiella burnetii: A Review of Available Methods with Major Focus on PCR-Based Techniques / V. Boldis, E. Spitalska and R. Toman.
  • 2013 Springer
    Susanne Modrow, Dietrich Falke, Uwe Truyen, Hermann Schätzl.
    The book gives a comprehensive overview on the knowledge of virus infection relevant for humans and animals. For each virus family the molecular details of the virus particle and the viral replication cycle are described. In the case of virus types with relevance for human and/or animal health the data on molecular biology, genetics and virus-cell interaction are combined with those concerning, pathogenesis, epidemiology, clinics, prevention and therapy.
  • 2013
    John Kuriyan, Boyana Konforti, David Wemmer.
    Status: Not Checked OutLane Catalog Record
    "Key to making future advances in the areas of biochemistry and molecular medicine is a new generation of molecular biologists and biochemists who are able to harness the tools and insights of physics and chemistry to exploit the emergence of genomic and systems-level information in biology.The basic ideas of energy, entropy, equilibrium thermodynamics, transport processes and reaction kinetics are closely related to exciting issues in contemporary biology, such as protein folding, chaperones and prion diseases, DNA polymerase and ribosome fidelity, DNA recognition, drug design, signal transduction, ion channel function, motor protein action and the versatility of enzyme mechanism." ... "Molecules of Life" ... deepens our understanding of how life functions by illuminating the physical principles underpinning biological phenomena." --Excerpts from publisher notes.
  • 2014 Springer Protocols
    edited by Shree Ram Singh, Vincenzo Coppola.
  • 2015 Springer Protocols
    edited by Robert Weissert.
    Positional gene cloning in experimental populations / Maja Jagodic and Pernilla Stridh - - Generation of transgenic rats using lentiviral vectors / Holger M. Reichard and Henrike J. Fischer -- DNA vaccination techniques / Nicolas Fissolo, Xavier Montalban, and Manuel Comabella -- Peripheral blood mononuclear cells : isolation, freezing, thawing, and culture / Christine Riedhammer, Dagmar Halbritter, and Robert Weissert -- ELISPOT techniques / Niannian Ji and Thomas G. Forsthuber -- Isolation of central nervous system (CNS) infiltrating cells / Ilgiz A. Mufazalov and Ari Waisman -- Methods for ex vivo analysis of immune cell function from the central nervous system / Darryl G. Turner ... [et al.] -- Neurofilament light chain determination from peripheral blood samples / Marguerite Limberg ... [et al.] -- Detection of autoantibodies against myelin oligodendrocyte glycoprotein in multiple sclerosis and related diseases / Melania Spadaro and Edgar Meinl -- Myelinating cultures : an in vitro tool to identify demyelinating and axopathic autoantibodies / Maren Lindner and Christopher Linington -- In vivo visualization of (auto-) immune processes in the central nervous system of rodents / Christian Schlager ... [et al.] -- Optical coherence tomography to assess neurodegeneration in multiple sclerosis / Axel Petzold -- Experimental autoimmune encephalomyelitis in mice / Rachael L. Terry, Igal Ifergan, and Stephen D. Miller -- Actively induced experimental autoimmune encephalomyelitis in rats / Robert Weissert -- Experimental autoimmune encephalomyelitis in marmosets / S. Anwar Jagessar ... [et al.]-- Neuropathological techniques to investigate CNS pathology in experimental autoimmune encephalomyelitis (EAE) / Karin Steinbach and Doron Merkler -- Neuropathological techniques to investigate central nervous system sections in multiple sclerosis / Jan Bauer and Hans Lassmann.
  • Springer Protocols
    edited by Katherine M.D. Bushby and Louise V.B. Anderson.
    Application of Molecular Methodologies in Muscular Dystrophies / Katharine M. Bushby, Louise V. Anderson -- Clinical Examination as a Tool for Diagnosis: Historical Perspective / David Gardner-Medwin -- Histopathological Diagnosis of Muscular Dystrophies / Margaret A. Johnson -- Serum Creatine Kinase in Progressive Muscular Dystrophies / Mayana Zatz, Mariz Vainzof, Maria Rita Passos-Bueno -- Deletion and Duplication Analysis in Males Affected with Duchenne or Becker Muscular Dystrophy / Ann Curtis, Daisy Haggerty -- Point Mutation Detection in the Dystrophin Gene / Johan T. Dunnen -- DNA-Based Techniques for Detection of Carriers of Duchenne and Becker Muscular Dystrophy / Egbert Bakker -- Fluorescence In Situ Hybridization Analysis for Carrier Detection in Duchenne/Becker Muscular Dystrophy / Jonathan K. Dore, Helen M. Kingston -- DNA- Based Prenatal Diagnosis for Duchenne and Becker Muscular Dystrophy / Ann Curtis, Daisy Haggerty -- Molecular Diagnosis and Genetic Counseling of the Manifesting Carrier of Duchenne Muscular Dystrophy / Eric P. Hoffman, James Giron -- Mutation Analysis of X-Linked Emery-Dreifuss Muscular Dystrophy Gene / Daniela Toniolo.
  • 2013 Springer Protocols
    edited by Laura Soucek, Nicole M. Sodir.
  • 2008 Springer Protocols
    edited by Tanya Parish and Amanda Claire Brown.
    Isolation of Mycobacterium species genomic DNA / John T. Belisle, Spencer B. Mahaffey, and Preston J. Hill -- Isolation of mycobacterial RNA / Tige R. Rustad [and others] -- Lipoglycans of Mycobacterium tuberculosis : isolation, purification, and characterization / Libin Shi, Jordi B. Torrelles, and Delphi Chatterjee -- Analyzing lipid metabolism : activation and [beta]-oxidation of fatty acids / Paul Robert Wheeler -- Whole genome analysis using microarrays / S.J. Waddell, J. Hinds, and P.D. Butcher -- Use of DNA microarrays to study global patterns of gene expression / Roberta Provvedi, Giorgio Palù, and Riccardo Manganelli -- Two-dimensional gel electrophoresis-based proteomics of mycobacteria / Jens Mattow [and others] -- Transport assays and permeability in pathogenic mycobacteria / Marie-Antoinette Lanéelle and Mamadou Daffé -- Continuous culture of mycobacteria / Joanna Bacon and Kim A. Hatch -- Measuring minimum inhibitory concentrations in mycobacteria / Frederick A. Sirgel, Ian J.F. Wiid, and Paul D. van Helden -- Rapid screening of inhibitors of Mycobacterium tuberculosis growth using tetrazolium salts / Anita G. Amin [and others] -- Electroporation of mycobacteria / Renan Goude and Tanya Parish -- Ins and outs of mycobacterial plasmids / Farahnaz Movahedzadeh and Wilbert Bitter -- The use of temperature-sensitive plasmids in mycobacteria / Damien Portevin, Wladimir Malaga, and Christophe Guilhot -- Heterologous expression of genes in mycobacteria / James A. Triccas and Anthony A. Ryan -- Inducible expression systems for mycobacteria / Christopher M. Sassetti -- Assaying promoter activity using LacZ and GFP as reporters / Paul Carroll and Jade James -- Construction of unmarked deletion mutants in mycobacteria / Houhui Song, Frank Wolschendorf, and Michael Niederweis -- Construction of targeted mycobacterial mutants by homologous recombination / Sharon L. Kendall and Rosangela Frita -- Phage transposon mutagenesis / M. Sloan Siegrist and Eric J. Rubin -- Gene essentiality testing in Mycobacterium smegmatis using specialized transduction / Apoorva Bhatt and William R. Jacobs, Jr. -- Gene switching and essentiality testing / Amanda Claire Brown -- Insertion element IS6110-based restriction fragment length polymorphism genotyping of Mycobacterium tuberculosis / Robin M. Warren, Paul D. van Helden, and Nicolaas C. Gey van Pittius -- Typing Mycobacterium tuberculosis using variable number tandem repeat analysis / T.J. Brown, V.N. Nikolayevskyy, and F.A. Drobniewski -- Molecular detection of drug-resistant Mycobacterium tuberculosis with a scanning-frame oligonucleotide microarray / Dmitriy V. Volokhov [and others] -- MycoDB : an online database for comparative genomics of the mycobacteria and related organisms / Roy R. Chaudhuri -- Environmental amoebae and mycobacterial pathogenesis / Melanie Harriff and Luiz E. Bermudez.
  • Springer Protocols
    edited by Tanya Parish and Neil G. Stoker.
    Plasmid vectors / N. Casali and S. Ehrt -- Isolation of DNA from Mycobacterium tubercolosis / P.D. Helden [and others] -- Extraction of RNA from intracellular Mycobacterium tuberculosis / I.M. Monahan, J.A. Mangan and P.D. Butcher -- Transposon mutagenesis in mycobacteria using conditionally replicating mycobacteriophages / S.S. Bardarov, S.S. Bardarov and W.R. Jacobs -- Gene replacement and transposon delivery using the negative selection marker sac B / M. Jackson [and others] -- Gene replacement using pretreated DNA / B.G. Gordhan and T. Parish -- Gene replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG using rpsl as a dominant negative selectable marker / P. Sander, B. Springer and E.C. Böttger -- Transcription start-site mapping / F. Movahedzadeh, J.A. Gonzalez-Y-Merchand and R.A. Cox -- Fluorescence and Brightfield cytology of live M. tuberculosis cells / R.P. Cooney, N.J. Garton and M.R. Barer -- Phage replication technology for diagnosis and drug susceptibility testing / R. McNerney -- Detection of mutations in Mycobacterium tuberculosis by a dot blot hybridization strategy / T.C. Victor and P.D. Helden -- Restriction fragment length polymorphism typing of mycobacteria / D. Soolingen, P.E.W. Haas and K. Kremer -- Preparation of culture filtrate proteins from Mycobacterium tuberculosis / I. Rosenkrands and P. Andersen -- Analysis of the capsule of Mycobacterium tuberculosis / M. Daffé and M.-A. Lanée -- Analysis of the lipids of Mycobacterium tuberculosis / R.A. Slayden and C.E. Barry -- In vitro model of hypoxically induced nonreplicating persistence of Mycobacterium tuberculosis / L.G. Wayne -- 17 macrophage virulence assays / P.T. Lukey and E.U. Hooker -- Analysis of mycobacterium -infected macrophages by immunoelectron microscopy and cell fractionation / W. Beatty and D.G. Russell -- Real time PCR using molecular beacons / R. Manganelli, S. Tyagi and I. Smith -- Electronic access to Mycobacterium tuberculosis sequence data / J. Parkhill -- Proteomics / J.C. Betts and M.A. Smith -- Functional genomics of Mycobacterium tuberculosis using DNA microarrays / M. Wilson [and others] -- Storage of mycobacterial strains / K. Kremer, T. Laan and D. Soolingens -- Safety in the laboratory / H. Alderton and D. Smith.
  • 2012 CRCnetBASE
    Yuri M. Yevdokimov, V.I. Salyanov, S.G. Skuridin.
    "Written for chemists, physicists, molecular biologists, and students in related fields, this reference covers information presently available on the specific properties of metallic nanoparticles, and compares their properties with those related to nanoobjects formed by biological molecules. It also discloses details of formation and physic-chemical peculiarities of the DNA nanostructures and DNA-based nanoconstructions. Timely in publication, the reference contains more DNA physics and molecular biology than other published titles. Authors discuss how nucleic acid molecules and their complexes with chemical and biologically active compounds are of great interest in the development of various nanoscale systems and devices of practical importance"-- Provided by publisher.
  • 2014 CRCnetBASE
    edited by Carla Mucignat-Caretta, Department of Molecular Medicine, University of Padova, Padova, Italy.
    Chapter 1. Introduction to chemical signaling in vertebrates and invertebrates / Tristram D. Wyatt -- chapter 2. Pheromones and general odor perception in insects / Michel Renou -- chapter 3. First investigation of the semiochemistry of South African dung beetle species / Barend (Ben) Victor Burger -- chapter 4. Pheromone reception in insects : the example of silk moths / Karl-Ernst Kaissling -- chapter 5. Chemical communication in the honey bee society / Laura Bortolotti and Cecilia Costa -- chapter 6. Drosophila pheromones : from reception to perception / Wynand van der Goes van Naters -- chapter 7. How drosophila detect volatile pheromones : signaling, circuits, and behavior / Samarpita Sengupta and Dean P. Smith -- chapter 8. Chemical signaling in amphibians / Sarah K. Woodley -- chapter 9. Vomeronasal organ : a short history of discovery and an account of development and morphology in the mouse / Carlo Zancanaro -- chapter 10. Vomeronasal receptors and signal transduction in the vomeronasal organ of mammals / Simona Francia, Simone Pifferi, Anna Menini, and Roberto Tirindelli -- chapter 11. Central processing of intraspecific chemical signals in mice / Carla Mucignat-Caretta -- chapter 12. Molecular and neural mechanisms of pheromone reception in the rat vomeronasal system and changes in the pheromonal reception by the maturation and sexual experiences / Makoto Kashiwayanagi -- chapter 13. Social cues, adult neurogenesis, and reproductive behavior / Paolo Peretto and Raúl G. Paredes -- chapter 14. Influence of cat odor on reproductive behavior and physiology in the house mouse (mus musculus) / Vera V. Voznessenskaya -- chapter 15. Pheromone of tiger and other big cats / Mousumi Poddar-Sarkar and Ratan Lal Brahmachary -- chapter 16. Cattle pheromones / Govindaraju Archunan, Swamynathan Rajanarayanan, and Kandasamy Karthikeyan -- chapter 17. Pheromones for newborns / Benoist Schaal -- chapter 18. Pheromone processing in relation to sex and sexual orientation / Ivanka Savic -- chapter 19. Human pheromones : do they exist? / Richard L. Doty.
  • 2010 Springer
    Gene J. Blatt, editor.
    Autism Spectrum Disorders: Clinical and Medical Perspectives -- The Male Prevalence in Autism Spectrum Disorders: Hypotheses on its Neurobiological Basis -- Neuroanatomical-Behavioral Correlates in Autism: A Working Hypothesis -- The Morphology of Minicolumns -- The Developmental Neuropathology of Autism -- Understanding Alterations During Human Brain Development with Molecular Imaging: Role in Determining Serotonin and GABA Mechanisms in Autism -- Glutamic Acid Decarboxylase (GAD) as a Biomarker of GABAergic Activity in Autism: Impact on Cerebellar Circuitry and Function -- Epigenetic Dysregulation of 15q11-13 GABAA Receptor Genes in Autism -- Cholinergic Component of Autism Spectrum Disorder -- Oxytocin and Autism -- The Role of the Noradrenergic System in Autism Spectrum Disorders -- Oxidative Stress in Autism and Its Implications for Dopamine-Stimulated Phospholipid Methylation -- Neuroligins and Neurexins: Synaptic Bridges Implicated in Autism -- The Neurophysical Chemistry of Autism: Postulates from Intelligence Modeling -- Pharmacological Treatment of Autism.
  • 2012 Springer
    Meena Upadhyaya, David N. Cooper, editors.
    Neurofibromatosis type 1 (NF1), caused by mutational inactivation of the NF1 tumour suppressor gene, is one of the most common dominantly inherited human disorders, affecting 1 in 3000 individuals worldwide. This book presents in concise fashion, but as comprehensively as possible, our current state of knowledge on the molecular genetics, molecular biology and cellular biology of this tumour predisposition syndrome. Written by internationally recognized experts in the field, the 44 chapters that constitute this edited volume provide the reader with a broad overview of the clinical features of the disease, the structure and expression of the NF1 gene, its germ line and somatic mutational spectra and genotype-phenotype relationships, the structure and function of its protein product (neurofibromin), NF1 modifying loci, the molecular pathology of NF1-associated tumours, animal models of the disease, psycho-social aspects and future prospects for therapeutic treatment.Neurofibromatosis Type 1: Molecular and Cellular Biology will be of great value to medical geneticists, molecular and cellular biologists, oncologists, dermatologists, neurologists, genetic counsellors and general practitioners alike.
  • 2015 Springer Protocols
    edited by Laura Lossi and Adalberto Merighi.
    Neuronal cell death : an overview of its different forms in central and peripheral neurons / Laura Lossi, Claudia Castagna, and Adalberto Merighi -- Cell volume regulation monitored with combined epifluorescence and digital holographic microscopy / Nicolas Pavillon and Pierre Marquet -- Flow cytometric analysis of DNA synthesis and apoptosis in central nervous system using fresh cell nuclei / Noelia López-Sánchez and José M. Frade -- Nuclear signs of pre-neurodegeneration / Fernando C. Baltanás ... [et al.] -- Multi-parametric O2 imaging in three-dimensional neural cell models with the phosphorescent probes / Ruslan I. Dmitriev and Dmitri B. Papkovsky -- Calcium imaging in neuron cell death / María Calvo, Carlos Villalobos, and Lucía Núñez -- Monitoring mitochondrial membranes permeability in live neurons and mitochondrial swelling through electron microscopy analysis / Macarena S. Arrázola and Nibaldo C. Inestrosa -- Real-time visualization of caspase-3 activation by fluorescence resonance energy transfer (FRET) / Silvia Alasia ... [et al.] -- Design and cloning of short hairpin RNAs (shRNAs) into a lentiviral silencing vector to study the function of selected proteins in neuronal apoptosis / Nadia Canu -- Genomic analysis using affymetrix standard microarray genechips (169 format) in degenerate murine retina / Sook Hyun Chung , Weiyong Shen , and Mark Gillies -- Genomic analysis of transcriptional changes underlying neuronal apoptosis / Sebastiano Cavallaro -- High-throughput cell death assays / Matthew E. Pamenter and Gabriel G. Haddad -- Staining of dead neurons by the golgi method in autopsy material / Stavros J. Baloyannis -- Image analysis algorithms for immunohistochemical assessment of cell death / Stan Krajewski ... [et al.] -- In vitro oxygen-glucose deprivation to study ischemic cell death / Carla I. Tasca, Tharine Dal-Cim, and Helena Cimarosti -- Laser microbeam targeting of single nerve axons in cell culture / Nicholas Hyun , Linda Z. Shi , and Michael W. Berns -- Real-time imaging of retinal cell apoptosis by confocal scanning laser ophthalmoscopy / Eduardo M. Normando ... [et al.] -- Targeted toxicants to dopaminergic neuronal cell death / Huajun Jin ... [et al.] -- Stem cells, neural progenitors, and engineered stem cells / Raj R. Rao and Shilpa Iyer -- Herpes simplex virus type 1 (HSV-1)-derived recombinant vectors for gene transfer and gene therapy / Peggy Marconi , Cornel Fraefel, and Alberto L. Epstein -- Herpes simplex virus type 1 (HSV-1)-derived amplicon vectors for gene transfer and gene therapy / Cornel Fraefel Peggy Marconi, and Alberto L. Epstein -- Bone marrow transplantation for research and regenerative therapies in the central nervous system / David Díaz, José Ramón Alonso, and Eduardo Weruaga -- Detection of activated caspase-8 in injured spinal axons by using fluorochrome-labeled inhibitors of caspases (FLICA) / Antón Barreiro-Iglesias and Michael I. Shifman -- Generation of zebrafish models by CRISPR/Cas9 genome editing / Alexander Hruscha and Bettina Schmid -- In vivo assessment of neuronal cell death in Drosophila / Pierre Dourlen -- Drosophila model for studying phagocytosis following neuronal cell death / Boris Shklyar, Flonia Levy-Adam, and Estee Kurant.
  • 2012 Springer
    Serge Morand, François Beaudeau, Jacques Cabaret, editors.
    Methods and tools in molecular epidemiology -- Molecular typing of bacterial pathogens: a tool for the epidemiological study and control of infectious diseases -- Contribution of population genetics -- Molecular epidemiology and evolutionary genetics of infectious diseases -- Population genetics and molecular epidemiology of infectious diseases -- Contribution of population and evolutionary epidemiology -- Modelling the dynamics of host-parasite interactions: basic principles -- Evolution of virulence: intuitions and models -- Case studies -- Molecular epidemiology of tuberculosis -- Pneumocystis molecular phylogeny: a way to understand both pneumocystosis natural histry and host taxonomy -- Hantavirus genetic diversity -- New integrations -- Nasal carriage of methicillin-resistant coagulase-negative staphylocococci: a reservoir of mecA gene for Staphylococcus aureus -- Molecular knowledge of mechanisms of helminth resistance: importance for diagnostic and epidemiology -- Molecular epidemiology of disease resistance genes with perspectives for researches on biological invasions and hybrid zones -- How does biodiversity influence the ecology of infectious disease? -- Epidemiological interaction at the wildlife/livestock/human interface: can we anticipate emerging infectious diseases in their hotspots? A framework for understanding emerging diseases processes in their hot spots.
  • 2009 Springer
    T. Tachikawa, K. Nose, T. Ohmori, M. Adachi, (eds.).
  • 2013 Springer
    Wei Wu, Hani Choudhry, editors.
    Introduction: next generation sequencing technology and cancer research -- The majority of total nuclear-encoded non-ribosomal RNA in a human cell is dark matter unannotated RNA -- Total RNA-seq of breast cancer in hypoxia -- Altered antisense-to-sense transcript ratios in breast cancer -- Identification of piRNAs in Hela cells by massive parallel sequencing -- Discovery of new microRNAs by small RNAome deep sequencing in childhood acute lymphoblastic leukemia -- Whole-Exome Sequencing Identifies FAM20A Mutations as a Cause of Amelogenesis Imperfecta and Gingival Hyperplasia Syndrome -- Whole-exome sequencing in CIC and IDH1/2 contributing to human oligodendroglioma -- Genetic and structural variation in the gastric cancer kinome revealed through targeted deep sequencing -- Tumour evolution inferred by single-cell sequencing -- Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq -- Tracing the derivation of embryonic stem cells from the inner cell mass by single-cell RNASeq analysis -- Whole genome DNA methylation analysis based on high throughput sequencing technology -- Comparative methylome analysis of benign and malignant peripheral nerve sheath tumors -- High-resolution genome-wide mapping of HIF-binding sites by ChIP-seq -- MicroRNA transfection and AGO-bound CLIP-seq data sets reveal distinct determinants of miRNA action -- Genome-wide identification of polycomb-associated RNAs by RIP-seq -- Single-molecule sequencing: sequence methods to enable accurate quantisation -- Metabolic labeling of RNA uncovers principles of RNA production and degradation dynamics in mammalian cells -- Reprogramming transcription by distinct classes of enhancers functionally defined by eRNA -- The genome information process for cancer research: the challenge and perspective.
  • 2011 Springer Protocols
    edited by Helen O. McCarthy, Jonathan A. Coulter.
    Nitric oxide physiology and pathology / David G. Hirst and Tracy Robson -- Ionizing radiation-induced DNA strand breaks and [gamma]-H2AX foci in cells exposed to nitric oxide / Kai Rothkamm and Susanne Burdak-Rothkamm -- Determination of S-nitrosothiols in biological fluids by chemiluminescence / Enika Nagababu and Joseph M. Rifkind -- Measurement of nitrite in blood samples using the ferricyanide-based hemoglobin oxidation assay / Barbora Piknova and Alan N. Schechter -- Selective fluorescent activation for bioimaging the expression of nitric oxide in cellular and in vivo systems / Junfeng Zhang and Hao Hong -- Real-time measurement of murine hippocampus NO levels in response to cerebral ischemia/reperfusion / Xiaoxiang Zheng, Kezhou Liu, and Yong Yang -- Detection of low levels of nitric oxide using an electrochemical sensor / Yong Chool Boo ... [et al.] -- Determination of the scavenging capacity against reactive nitrogen species by automatic flow injection-based methodologies / Marcela A. Segundo ... [et al.] -- Aqueous measurement of nitric oxide using membrane inlet mass spectrometry / David N. Silverman and Chingkuang Tu -- Quantum cascade laser technology for the ultrasensitive detection of low-level nitric oxide / Angela Elia ... [et al.] -- Determination of in vivo nitric oxide levels in animal tissues using a novel spin trapping technology / Anatoly F. Vanin and Alexander A. Timoshin -- [Beta] cell protection by inhibition of iNOS through lentiviral vector-based strategies / Sean O. Hynes, Cillian McCabe, and Timothy O'Brien -- Characterization of nitric oxide delivery systems produced by various nanotechnologies / Chi H. Lee -- Nitric oxide releasing nanoparticle synthesis and characterization / George Han, Adam J. Friedman, and Joel M. Friedman -- NOS antagonism using viral vectors as an experimental strategy : implications for in vivo studies of cardiovascular control and peripheral neuropathies / Beihui Liu ... [et al.]
  • 2011 Springer Protocols
    edited by Markus W. Ribbe.
    Historic overview of nitrogenase research / Yilin Hu and Markus W. Ribbe -- Mechanism of mo-dependent nitrogenase / Zhi-Yong Yang, Karamatullah Danyal, and Lance C. Seefeldt -- Assembly of nitrogenase MoFe protein / Chi-Chung Lee -- Genomic analysis of nitrogen fixation / Ina P. O'Carroll and Patricia C. Dos Santos -- Enzymatic systems with homology to nitrogenase / Jurgen Moser and Markus J. Brocker -- Molecular biology and genetic engineering in nitrogen fixation / Patricia C. Dos Santos -- Purification of nitrogenase proteins / Jared A. Wiig [and others] -- Assays of nitrogenase reaction products / William E. Newton and Michael J. Dilworth -- Methods for nitrogenase-like dark operative protochlorophyllide oxidoreductase / Jurgen Moser and Markus J. Brocker -- X-ray crystallography / Lauren E. Roth and F. Akif Tezcan -- X-ray absorption spectroscopy / Serena DeBeer -- Small angle x-ray scattering spectroscopy / David W. Mulder and John W. Peters -- Electron paramagnetic resonance spectroscopy / Karamatullah Danyal, Zhi-Yong Yang, and Lance C. Seefeldt -- Magnetic circular dichroism spectroscopy / Brian J. Hales -- Mössbauer spectroscopy / Boi Hanh Huynh -- Protocols for cofactor isolation of nitrogenase / Aaron W. Fay [and others] -- Techniques for functional and structural modeling of nitrogenase / Patrick L. Holland -- Nitrogenase structure and function relationships by density functional theory / Travis V. Harris and Robert K. Szilagyi -- Modeling the MoFe nitrogenase system with broken symmetry density functional theory / Gregory M. Sandala and Louis Noodleman.
  • 2010
    Michael Paul Bokoch.
    G protein coupled receptors (GPCRs) are seven transmembrane proteins that are expressed in all eukaryotic cells and tissues. These receptors play key roles in human physiology and disease. The goal of my work is to understand the molecular detail of ligand recognition by GPCRs, and how this process leads to conformational changes that manifest as cellular signaling. Meeting this goal will advance our knowledge of membrane protein biology. It will also reveal structural targets and physicochemical logic to aid pharmaceutical design. The age of GPCR structural biology recently arrived with the first x-ray structures of rhodopsin and the beta2 adrenergic receptor (beta2AR). However, membrane proteins are constantly fluctuating entities. Dynamic behavior is intrinsic to their function. As such, static x-ray structures alone are inadequate. Herein, I develop biophysical techniques to study these dynamic receptors. Using NMR spectroscopy, I characterize conformational changes in the extracellular region of the beta2AR, a surface rich with potential for drug design. I also explore the signaling properties of monomeric GPCRs and conformational changes of other macromolecules using single-molecule fluorescence. While many questions about GPCRs remain, I hope this work is a small step towards understanding these important, fascinating, and beautiful molecules.
  • 2005 CRCnetBASE
    edited by A. Ramamoorthy.
  • 2006 CRCnetBASE
    edited by Qiang Cui, Ivet Bahar.
  • 2015 Springer Protocols
    edited by Shinichi Nakagawa, Tetsuro Hirose.
    Visualization of lncRNA by single-molecule fluorescence in situ hybridization / Margaret Dunagin , Moran N. Cabili , John Rinn , and Arjun Raj -- Super-resolution imaging of nuclear bodies by STED microscopy / Yasushi Okada and Shinichi Nakagawa -- High-resolution 3D DNA FISH using plasmid probes and computational correction of optical aberrations to study chromatin structure at the sub-megabase scale / Luca Giorgetti, Tristan Piolot, and Edith Heard -- Time-lapse imaging of nuclear bodies / Saskia Hutten, Samuel Swift, and Angus I. Lamond -- Visualization of nucleic acids with synthetic exciton-controlled fluorescent oligonucleotide probes / Dan Ohtan Wang and Akimitsu Okamoto -- Live CLEM imaging to analyze nuclear structures at high resolution / Tokuko Haraguchi, Hiroko Osakada, and Takako Koujin -- Ultrastructural analysis of nuclear bodies using electron microscopy / Sylvie Souquere and Gérard Pierron -- Analyses of nuclear proteins and nucleic acid structures using atomic force microscopy / Jamie L. Gilmore [and eight others] -- Genome-wide co-localization screening of nuclear body components using a fluorescently tagged FLJ cDNA clone library / Tetsuro Hirose and Naoki Goshima -- Purification of specific chromatin regions using oligonucleotides : capture hybridization analysis of RNA targets (CHART) / Christopher P. Davis and Jason A. West -- RNA antisense purification (RAP) for mapping RNA interactions with chromatin / Jesse Engreitz, Eric S. Lander, and Mitchell Guttman -- In situ dissection of RNA functional subunits by domain-specific chromatin isolation by RNA purification (dChIRP) / Jeffrey J. Quinn and Howard Y. Chang -- Extracting, enriching, and identifying nuclear body sub-complexes using label-based quantitative mass spectrometry / Archa Fox [and three othrs] -- Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells : native ribonucleoprotein immunoprecipitation (RIP) assays / Joseph A. Cozzitorto [and eight others] -- Cross-linking and immunoprecipitation of nuclear RNA-binding proteins / Quan Li , Yuri Uemura , and Yukio Kawahara -- Purification of noncoding RNA and bound proteins using FLAG peptide-conjugated antisense-oligonucleotides / Shungo Adachi and Tohru Natsume -- MMCT-mediated chromosome engineering technique applicable to functional analysis of lncRNA and nuclear dynamics / Makiko Meguro-Horike and Shin-ichi Horike -- Reconstitution of nucleocytoplasmic transport using digitonin-permeabilized cells / Shingo Kose, Tomoko Funakoshi, and Naoko Imamoto -- Genome-wide analysis of long noncoding RNA turnover / Hidenori Tani [and eight others] -- Knockdown of nuclear-retained long noncoding RNAs using modified DNA antisense oligonucleotides / Xinying Zong [and six others] -- siRNA screening of nuclear proteins / Yuko Hasegawa and Shinichi Nakagawa.
  • 2007 Springer
    K. Nagata, K. Takeyasu (eds.).
  • 2015 Springer Protocols
    edited by Bruce G. Allan, Terence E. Hébert
    Studies of intracellular angiotensin II / Richard N. Re and Julia L. Cook -- Single-cell microinjection coupled to confocal microscopy to characterize nuclear membrane receptors in freshly isolated cardiomyocytes / Clémence Merlen and Jonathan Ledoux -- Design and application of light-activated probes for cellular signaling / David Chatenet, Steve Bourgault, and Alain Fournier -- Using caged ligands to study intracrine endothelin signaling in intact cardiac myocytes / Clémence Merlen, Louis R. Villeneuve, and Bruce G. Allen -- Quantification of catecholamine uptake in adult cardiac myocytes / Erika F. Dahl, Casey D. Wright, and Timothy D. O'Connell -- Characterization of the interaction between the prostaglandin D2 DP1 receptor and the intracellular L-Prostaglandin D synthase / Chantal Binda and Jean-Luc Parent -- Isolation and study of cardiac nuclei from canine myocardium and adult ventricular myocytes / Artavazd Tadevosyan, Bruce G. Allen, and Stanley Nattel -- High resolution imaging and function of nuclear G protein-coupled receptors (GPCRs) / Vikrant K. Bhosle [and others] -- Biochemical fractionation of membrane receptors in the nucleus / Ying-Nai Wang ... [et at.] -- Functional G protein-coupled Receptors on nuclei from brain and primary cultured neurons / Yuh-Jiin I. Jong and Karen L. O'Malley -- Automated microscopy of cardiac myocyte hypertrophy : a case study on the role of intracellular [alpha]-adrenergic receptors / Karen A. Ryall and Jeffrey J. Saucerman -- Measuring intranuclear and nuclear envelope [Ca2+] vs. cytosolic [Ca2+] / Senka Ljubojević and Donald M. Bers -- Assessing GPCR and G protein signaling to the nucleus in live cells using fluorescent biosensors / Julie Bossuyt and Donald M. Bers -- Tandem affinity purification to identify cytosolic and nuclear G [beta-gamma]-interacting proteins / Rhiannon Campden [and others] -- Examining the effects of nuclear GPCRs on gene expression using isolated nuclei / George Vaniotis [and others] -- Trafficking and function of GPCRs in the endosomal compartment / Davide Calebiro [and others].
  • 2010 Cold Spring Harb Lab Press
    meeting organized by Terri Grodzicker, David Spector, David Stewart, and Bruce Stillman.
    Chromatin modifications and gene expressions -- Transcription and beyond -- DNA replication and genome integrity -- Reprogramming and differentiation -- Heterochromatin formation and gene silencing -- Nuclear bodies and RNA biology -- Chromosome structure and mitosis -- Chromatin structure and organization -- Nuclear periphery.
  • 2013 Springer Protocols
    edited by Dmitry M. Kolpashchikov and Yulia V. Gerasimova.
    Getting blood from a stone : ultrasensitive forensic DNA profiling of microscopic bio-particles recovered from touch DNA evidence / Erin K. Hanson and Jack Ballantyne -- Detecting microRNA in human cancer tissues with fluorescence in situ hybridization Zonggao Shi, Jeff J. Johnson, and M. Sharon Stack -- MNAzyme qPCR : a superior tool for multiplex qPCR / Elisa Mokany and Alison V. Todd -- New, multiplex, quantitative real-time polymerase chain reaction system for nucleic acid detection and quantification / Fang Liang ... [et al.] -- Detection of SNP-containing human DNA sequences using a split sensor with a universal molecular beacon reporter / Yulia V. Gerasimova, Jack Ballantyne, and Dmitry M. Kolpashchikov -- SNP analysis using a molecular beacon-based operating cooperatively (OC) sensor / Evan M. Cornett and Dmitry M. Kolpashchikov -- Detection of rpoB gene mutations using helicase- dependent amplification / Wanyuan Ao and Robert Jenison -- Rapid detection of brucella spp. using loop-mediated isothermal amplification (LAMP) / Shouyi Chen ... [et al.] -- Loop-mediated isothermal amplification method for a differential identification of human taenia tapeworms / Yasuhito Sako ... [et al.] -- Detection of mutation by allele-specific loop-mediated isothermal amplification (AS-LAMP) / Hiroka Aonuma ... [et al.] -- DNA detection by cascade enzymatic signal amplification / Bingjie Zou, Yinjiao Ma, and Guohua Zhou -- Visual DNA detection and SNP genotyping using asymmetric PCR and split DNA enzymes / Jia Ling Neo and Mahesh Uttamchandani -- Detection of single-stranded nucleic acids via colorimetric means, using g-quadruplex probes / Herman O. Sintim and Shizuka Nakayama -- Lateral flow biosensors for the detection of nucleic acid / Lingwen Zeng ... [et al.] -- Electrochemiluminescence detection of c-Myc mRNA in breast cancer cells on a wireless bipolar electrode / Mei-Sheng Wu ... [et al.] -- High-throughput label-free detection of DNA hybridization and mismatch discrimination using interferometric reflectance imaging sensor / Sunmin Ahn ... [et al.] -- Graphene-PAMAM dendrimer-gold nanoparticle composite for electrochemical DNA hybridization detection / Kumarasamy Jayakumar ... [et al.] -- Application of PNA openers for fluorescence-based detection of bacterial DNA / Irina Smolina -- DNA probes for FISH analysis of C-negative regions in human chromosomes / Evgeniy S. Morozkin ... [et al.] -- Quantitative analysis of MicroRNA in blood serum with protein-facilitated affinity capillary electrophoresis / Maxim V. Berezovski and Nasrin Khan -- High-throughput functional microRNA profiling using recombinant AAV-based microRNA sensor arrays / Wenhong Tian ... [et al.] -- Use of molecular beacons to detect and quantify microRNA / Meredith B. Baker, Gang Bao, and Charles D. Searles -- Sequence-specific imaging of influenza A mRNA in living infected cells using fluorescent FIT-PNA / Susann Kummer ... [et al.] -- Application of caged fluorescent nucleotides to live-cell RNA imaging / Akimitsu Okamoto.
  • 2010 CRCnetBASE
    Raymond C. Valentine, David L. Valentine.
    Ch. 1. Molecular biology of Omega-3 chains as structural lipids : many central questions remain unanswered -- Ch. 2. Darwinian selection of the fittest membrane lipids : from archaeal isoprenoids to DHA-enriched rhodopsin disks -- Ch. 3. Coevolution of DHA membranes and their proteins -- Ch. 4. Convergent evolution of DHA/EPA biosynthetic pathways -- Ch. 5. Membrane evolution in a marine bacterium : capitalizing on DHA for energy conservation in seawater -- Ch. 6. Evolution of DHA membranes in human neutrons -- Ch. 7. DHA/EPA chains as powerful membrane antifreeze -- Ch. 8. DHA as a mediocre permeability barrier against cations : water wire theory -- Ch. 9. DHA/EPA membranes as targets of oxidative damage -- Ch. 10. Bacteria : environmental modulation of membrane lipids for bioenergetic gain -- Ch. 11. Chloroplasts : harnessing DHA/EPA for harvesting light in the sea -- Ch. 12. Mitochondria : DHA-cardiolipin boosts energy output -- Ch. 13. Sperm : essential roles of DHA lead to development of a mechanical stress hypothesis -- Ch. 14. DHA/EPA mutualism between bacteria and marine animals -- Ch. 15. Membrane adaptations for an oily environment : lessons from a petroleum-degrading bacterium -- Ch. 16. Lessons from yeast : phospholipid conformations are important in winemaking -- Ch. 17. DHA principle applied to global warming -- Ch. 18. DHA principle applied to molecular farming -- Ch. 19. DHA/unsaturation theory of aging -- Ch. 20. DHA principle applied to neurodegenerative diseases -- Ch. 21. Dietary DHA in prevention of colon cancer : how a risk to the cell benefits the organism.
  • 2012 CRCnetBASE
    edited by Debmalya Barh, Kenneth Blum, Margaret Anne Madigan.
    Section 1. Methodology and application -- section 2. Empirical research -- section 3. Computational and systems biology -- section 4. Therapeutics -- section 5. Future perspective.
  • 2014 CRCnetBASE
    edited by Yu Liu.
    Part 1. Omics and human disease -- part 2. Translational and personalized medicine -- part 3. Molecular and genetic markers -- part 4. Omics and pathogens -- part 5. Companion diagnostics.
  • by Victor M. Panaretos
  • 2008 Springer Protocols
    edited by Delphine Pflieger and Jean Rossier.
    Analysis of organelles by on-line two-dimensional liquid chromatography-tandem mass spectrometry / Edwin P. Romijn and John R. Yates III -- Purification and proteomic analysis of chloroplasts and their sub-organellar compartments / Daniel Salvi ... [et al.] -- Proteomic analysis of the stacked golgi complex / Mark S. Lowenthal, Kathryn E. Howell, and Christine C. Wu -- Purification of Saccharomyces cerevisiae mitochondria by zone electrophoresis in a free flow device / Hans Zischka ... [et al.] -- Preparation of respiratory chain complexes from Saccharomyces cerevisiae wild-type and mutant mitochondria : activity measurement and subunit composition analysis / Claire Lemaire and Geneviève Dujardin -- Mitochondrial proteomics : analysis of a whole mitochondrial extract with two-dimensional electrophoresis / Thierry Rabilloud -- Purification and proteomic analysis of the mouse liver mitochondrial inner membrane / Sandrine Da Cruz and Jean-Claude Martinou -- Subcellular fractionation and proteomics of nuclear envelopes / Laurence Florens, Nadia Korfali, and Eric C. Schirmer -- Purification and proteomic analysis of a nuclear-insoluble protein fraction / Tsuneyoshi Horigome, Kazuhiro Furukawa, and Kohei Ishii -- Preparation methods of human metaphase chromosomes for their proteome analysis / Kiichi Fukui, Hideaki Takata, and Susumu Uchiyama -- Purification and proteomic analysis of plant plasma membranes / Erik Alexandersson ... [et al.] -- Protocol to enrich and analyze plasma membrane proteins from frozen tissues / Jacek R. Wiśniewski -- Proteomic characterization of membrane protein topology / Adele R. Blackler and Christine C. Wu -- Free flow isoelectric focusing : a method for the separation of both hydrophilic and hydrophobic proteins of rat liver peroxisomes / Markus Islinger and Gerhard Weber -- Use of gas-phase fractionation to increase protein identifications : application to the peroxisome / Jacob Kennedy and Eugene C. Yi -- Purification and proteomic analysis of lysosomal integral membrane proteins / Huiwen Zhang ... [et al.] -- Affinity purification of soluble lysosomal proteins for mass spectrometric identification / Sylvie Kieffer-Jaquinod ... [et al.] -- Purification and proteomic analysis of synaptic vesicles / Holly D. Cox and Charles M. Thompson -- Purification and proteomics analysis of pancreatic zymogen granule membranes / Xuequn Chen and Philip C. Andrews -- Isolation and proteomic analysis of chlamydomonas centrioles / Lani C. Keller and Wallace F. Marshall -- Purification and proteomic analysis of 20S proteasomes from human cells / Marie-Pierre Bousquet-Dubouch ... [et al.] -- Characterization of E. coli ribosomal particles : combined analysis of whole proteins by mass spectrometry and of proteolytic digests by liquid chromatography-tandem mass spectrometry / Isabelle Iost ... [et al.] -- Assessment of organelle purity using antibodies and specific assays : the example of the chloroplast envelope / Daniel Salvi ... [et al.] -- Identifying bona fide components of an organelle by isotope-coded labeling of subcellular fractions : an example in peroxisomes / Marcello Marelli, Alexey I. Nesvizhskii, and John D. Aitchison -- Determination of genuine residents of plant endomembrane organelles using isotope tagging and multivariate statistics / Kathryn S. Lilley and Tom P.J. Dunkley -- Quantitative proteomic analysis to profile dynamic changes in the spatial distribution of cellular proteins / Wei Yan, Daehee Hwang, and Ruedi Aebersold -- Use of transcriptomic data to support organelle proteomic analysis / Wallace F. Marshall.
  • 2008
    Gérard Battail.
    A brief overview of molecular genetics -- An overview of information theory -- More on molecular genetics -- More on information theory -- An outline of error-correcting codes -- DNA is an ephemeral memory -- A toy living world -- Subsidiary hypothesis, nested system -- Soft codes -- Biological reality conforms to the hypotheses -- Identification of genomic codes.
  • 2006 MyiLibrary
    Managing editor, R. Cammack ; General editors, T.K. Attwood ... [et al.].
    Also available: Print – 2006
  • 2011 Springer
    [edited by] Ayeda Ayed, Theodore Hupp.
    TP53 mutations in human cancers : selection versus mutagenesis / Magali Olivier, Audrey Petitjean, Claude Caron de Fromentel, and Pierre Hainaut -- Lessons on p53 from mouse models / Dadi Jiang and Laura D. Attardi -- TP63, TP73 : the guardian's elder brothers / Stephanie Courtois, Pierre Hainaut and Claude Caron de Fromentel -- The regulation of p53 protein function by phosphorylation / Nicola J. Maclaine and Theodore Hupp -- The p53-Mdm2 loop : a critical juncture of stress response / Yaara Levav-Cohen, Zehavit Goldberg, Osnat Alsheich Bartok, Valentina Zuckerman, Sue Haupt and Ygal Haupt -- Cooperation between MDM2 and MDMX in the regulation of p53 / Jeremy Blaydes -- Regulation and function of the original p53-inducible p21 gene / Jennifer A. Fraser -- p53 localization / Carl G. Maki -- Modes of p53 interactions with DNA in the chromatin context / Vladana Vukojevic, Tatiana Yakovleva, and Georgy Bakalkin -- p53's dilemma in transcription : analysis by microarrays / Karuppiah Kannan, Gideon Rechavi and David Givol -- Tumor viruses and p53 / Nobuo Horikoshi -- p53 and immunity / Vikram Narayan, Sarah E. Meek and Kathryn L. Ball.
  • 2002 Springer Protocols
    edited by Bing-Yuan Chen and Harry W. Janes.
    A short history of the polymerase chain reaction /; J.M. Bartlett and D. Stirling --; Pcr patent issues /; P. Carroll and D. Casimir --; Equipping and establishing a pcr laboratory /; S. McDonagh --; Quality control in pcr /; D. Stirling --; Extraction of nucleic acid templates /; J.M. Bartlett --; Extraction of DNA from whole blood /; J.M. Bartlett and A. White --; DNA extraction from tissue /; H. Pearson and D. Stirling --; Extraction of DNA from microdissected archival tissues /; J.J. Going --; Rna extraction from blood /; H. Pearson --; Rna extraction from frozen tissue /; J.M. Bartlett --; Rna extraction from tissue sections /; H. Pearson --; Dual DNA/rna extraction /; D. Stirling and J.M. Bartlett --; DNA extraction from fungi, yeast, and bacteria /; D. Stirling --; Isolation of rna viruses from biological materials /; S. McDonagh --; Extraction of ancient DNA /; W.M. Schmerer --; DNA extraction from plasma and serum /; D. Stirling --; Technical notes for the detection of nucleic acids /; J.M. Bartlett --; Technical notes for the recovery and purification of pcr products from acrylamide gels /; D. Stirling --; Pcr primer design /; D.L. Hyndman and M. Mitsuhashi --; Optimization of polymerase chain reactions /; H. Grunenwald --; Subcycling pcr for long-distance amplifications of regions with high and low guanine-cystine content: Amplification of the intron 22 inversion of the fviii gene /; D. Stirling --; Rapid amplification of cdna ends /; X. Wang and W.S. Young, 3rd --; Randomly amplified polymorphic DNA fingerprinting: The basics /; R.S. Dassanayake and L.P. Samaranayake --; Microsphere-based single nucleotide polymorphism genotyping /; M.A. Iannone [and others] --; Ligase chain reaction /; W.H. Benjamin, Jr., K.R. Smith and K.B. Waites --; Nested rt-pcr in a single closed tube /; A. Olmos [and others] --; Direct pcr from serum: Application to viral genome detection /; K. Abe --; Long pcr amplification of large fragments of viral genomes: A technical overview /; R. Tellier [and others] --; Long pcr methodology /; R. Tellier [and others] --; Qualitative and quantitative pcr: A technical overview /; D. Stirling --; Ultrasensitive pcr detection of tumor cells in myeloma /; F.W. Cremer and M. Moos --; Ultrasensitive quantitative pcr to detect rna viruses /; S. McDonagh --; Quantitative pcr for camp ri alpha mrna: Use of site-directed mutation and pcr mimics /; J.M. Bartlett --; Quantitation of multiple rna species /; R. Kerr --; Differential display: A technical overview /; J.M. Bartlett --; Au-differential display, reproducibility of a differential mrna display targeted to au motifs /; O. Dominguez [and others] --; Pcr fluorescence differential display /; K. Khalturin, S. Kuznetsov and T.C. Bosch --; Microarray analysis using rna arbitrarily primed pcr /; S. Ringquist [and others] --; Oligonucleotide arrays for genotyping: Enzymatic methods for typing single nucleotide polymorphisms and short tandem repeats /; S. Case-Green, C. Pritchard and E. Southern --; Serial analysis of gene expression /; K.A. Oien --; Mutation and polymorphism detection: A technical overview /; J. Edwards and J.M. Bartlett --; Combining multiplex and touchdown pcr for microsatellite analysis /; K. Rithidech and J.J. Dunn --; Detection of microsatellite instability and loss of heterozygosity using DNA extracted from formalin-fixed paraffin-embedded tumor material by fluorescence-based multiplex microsatellite pcr /; J. Edwards and J.M. Bartlett --; Reduction of shadow band synthesis during pcr amplification of repetitive sequences from modern and ancient DNA /; W.M. Schmerer --; Degenerate oligonucleotide-primed pcr /; M. Aubele and J. Smida --; Mutation detection using rt-pcr-rflp /; H. Nakashima, M. Akahoshi and Y. Tanaka --; Multiplex amplification refractory mutation system for the detection of prothrombotic polymorphisms /; D. Stirling --; Pcr-sscp analysis of polymorphism: A simple and sensitive method for detecting differences between short segments of DNA /; M. Han and M.A. Robinson --; Sequencing: A technical overview /; D. Stirling --; Preparation and direct automated cycle sequencing of pcr products /; S.E. Daniels --; Nonradioactive pcr sequencing using digoxigenin /; S. Kosel [and others] --; Direct sequencing by thermal asymmetric pcr /; G.R. Mazars and C. Theillet --; Analysis of nucleotide sequence variations by solid-phase minisequencing /; A. Suomalainen and A.C. Syvanen --; Direct sequencing with highly degenerate and inosine-containing primers /; Z. Shen [and others] --; Determination of unknown genomic sequences without cloning /; J.P. Quivy and P.B. Becker --; Cloning pcr products for sequencing in m13 vectors /; D. Walsh --; DNA rescue by the vectorette method /; M.A. McAleer, A.J. Coffey and I. Dunham --; Technical notes for sequencing difficult templates /; D. Stirling --; Pcr-based detection of nucleic acids in chromosomes, cells, and tissues: Technical considerations on prins and in situ pcr and comparison with in situ hybridization /; E.J. Speel, F.C. Ramaekers and A.H. Hopman --; Cycling primed in situ amplification /; J.H. Bull and L. Paskins --; Direct and indirect in situ pcr /; K.H. Wiedorn and T. Goldmann --; Reverse transcriptase in situ pcr: New methods in cellular interrogation /; M. Gilchrist and A.D. Befus --; Primed in situ nucleic acid labeling combined with immunocytochemistry to simultaneously localize DNA and proteins in cells and chromosomes /; E.J. Speel, F.C. Ramaekers and A.H. Hopman --; Cloning and mutagenesis: A technical overview /; H. Pearson and D. Stirling --; Using t4 DNA polymerase to generate clonable pcr products /; K. Wang --; A t-linker strategy for modification and directional cloning of pcr products /; R.M. Horton, R. Raju and B.M. Conti-Fine --; Cloning gene family members using pcr with degenerate oligonucleotide primers /; G.M. Preston --; Cdna libraries from a low amount of cells /; P. Ravassard [and others] --; Creation of chimeric junctions, deletions, and insertions by pcr /; G. Pont-Kingdon --; Recombination and site-directed mutagenesis using recombination pcr /; D.H. Jones and S.C. Winistorfer --; Megaprimer pcr: Application in mutagenesis and gene fusion /; E. Burke and S. Barik.
  • 2002 Springer Protocols
    edited by Bimal D.M. Theophilus and Ralph Rapley.
    Agarose and polyacrylamide gel electrophoresis / Andrea M. Guilliatt -- Internal labeling of DNA probes / Ralph Rapley -- End-labeling of DNA probes / Adrian J. Harwood -- Southern blotting of agarose gels bu capillary transfer / Ralph Rapley -- Restriction fragment length polymorphism / Mohammad S. Enayat -- PCR: principles, procedures, and parameters / Nicola Louise Jones -- Allele-specific oligonucleotide PCR / Elaine K. Green -- Long-range PCR / Peter A. Davies -- Analysis of nucleotide sequence variations by solid-phase minisequencing / Anu Wartiovaara -- Cycle sequencing of PCR products / G.K. Surdhar -- Fluorescent in situ hybridization / Sara A. Dyer -- The protein truncation test / Carol A. Hardy -- Mutation detection in factor vii cDNA from lymphocytes of hemophilia A patients by solid phase fluorescent chemical cleavage of mismatch / Naushin H. Waseem -- Denaturing gradient gel electrophoresis / Yvonee Wallis -- Conformation-sensitive gel electrophoresis / Ian J. Williams -- SSCp/heteroduplex analysis -- Cleavase fragment length polymorphism analysis for genotyping and mutation detection / Laura Heisler -- Automated genotyping using the DNA MassArray technology / Christian Jurinke -- An introduction to bioinformatics / Henry Brzeski.
  • 2015 Springer Protocols
    edited by Chhandak Basu.
    "This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design , and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls." -- Provided by publisher.
  • Springer Protocols
    edited by Anton Yuryev.
  • 2013 Springer Protocols
    edited by Predrag Cudic.
    Hantzsch Based Macrocyclization Approach for the Synthesis of Thiazole Containing Cyclopeptides / Adel Nefzi -- The Chemical Synthesis of [alpha]-Conotoxins and Structurally Modified Analogs with Enhanced Biological Stability / Jayati Banerjee [and three other] -- Synthesis of AApeptides / Youhong Niu [and three others] -- Peptoids and Peptide-Peptoid Hybrid Biopolymers as Peptidomimetics / Maciej J. Stawikowski -- Synthesis of Side Chain N,N'-Diaminoalkylated Derivatives of Basic Amino Acids for Application in Solid-Phase Peptide Synthesis / Jean-Philippe Pitteloud, Nina Bionda, and Predrag Cudic -- Study Protein Folding and Aggregation Using Nonnatural Amino Acid p-Cyanophenylalanine as a Sensitive Optical Probe / Deguo Du, Haiyang Liu, and Bimlesh Ojha -- Adamantoylated Biologically Active Small Peptides and Glycopeptides Structurally Related to the Bacterial Peptidoglycan / Ruža Frkanec, Branka Vranešić, and Srdjanka Tomić -- Optimization of Physicochemical and Pharmacological Properties of Peptide Drugs by Glycosylation / Maria C. Rodriguez and Mare Cudic -- The Maillard Reaction Induced Modifications of Endogenous Opioid Peptide Enkephalin / Andreja Jakas -- Solid-Phase Guanidinylation of Peptidyl Amines Compatible with Standard Fmoc-Chemistry: Formation of Monosubstituted Guanidines -/ Nina Bionda and Predrag Cudic -- Stabilization of Collagen-Model,Triple-Helical Peptides for In Vitro and In Vivo Applications / Manishabrata Bhowmick and Gregg B. Fields -- Identification of Adipokine Receptor Agonists and Turning Them to Antagonists / Laszlo Otvos Jr. -- Peptide Detection and Structure Determination in Live Cells using Confocal Raman Microscopy / Andrew C. Terentis and Jing Ye.
  • 2002 Springer Protocols
    edited by Janet J. Maguire and Anthony P. Davenport.
    Immunocytochemical localization of endothelin peptides, precursors, and endothelin-converting enzymes / R.E. Kuc -- Analysis of endothelins by enzyme-linked immunosorbent assay and radioimmunoassay / A.P. Davenport and R.E. Kuc -- Separation of endothelin isoforms by high performance liquid chromatography and detection by radioimmunoassay / M.J. Ashby -- Radioligand binding assays and quantitative autoradiography of endothelin receptors / A.P. Davenport and R.E. Kuc -- Using receptor antagonists in binding studies to characterize a mammalian endothelin receptor / J.R. Wu-Wong -- Quantification of endothelin receptor mrna by competitive rt-pcr / P.J. Smith and J.C. Monge -- Detection and assessment of endothelin-converting enzyme activity / C.D. Jackson and A.J. Turner -- Quantitative measurement of mrna levels by rt-pcr. Studies of ece-1 isoforms / D.M. Lees [and others] -- Evaluation of endothelin-converting enzyme inhibitors using cultured cells / R. Corder -- Endothelin-converting enzyme activity in vascular smooth muscle preparations in vitro / J.J. Maguire -- Characterization of a new endothelin receptor ligand by in vitro assays / J.R. Wu-Wong -- Use of in vitro organ cultures of human saphenous vein as a model for intimal proliferation / K.E. Porter -- Investigation of the endothelin system in experimental heart failure / G.A. Gray and L. Sherry -- Local forearm vasoconstriction to endothelin-1 measured by venous occlusion plethysmography in vivo / F.E. Strachan and D.J. Webb.
  • 2009 Springer
    Dmitri V. Krysko, Peter Vandenabeele, editors.
  • 2008 Springer Protocols
    edited by Qing Yan.
  • 2005 Springer Protocols
    edited by Claire Lugnier.
    Purification of PDE6 isozymes from mammalian retina / Dana C. Pentia ... [et al.] -- Cyclic guanosine 5'-monophosphate binding to the regulatory GAF domains of photoreceptor phosphodiesterase / Rick H. Cote -- Renaturation of the catalytic domain of PDE4A expressed in escherichia coli as inclusion bodies / Wito Richter, Thomas Hermsdorf, and Dietrich Dettmer -- Determining the subunit structure of phosphodiesterases using gel filtration and sucrose density gradient centrifugation / Wito Richter -- Crystallization of cyclic nucleotide phosphodiesterases / Hengming Ke, Qing Huai, and Robert X. Xu -- Generation of PDE4 knockout mice by gene targeting / S.-L. Catherine Jin, Anne M. Latour, and Marco Conti -- Immunoprecipitating PDE2 phosphorylated and inactivated by an associated protein kinase / J. Kelley Bentley -- Investigation of extracellular signal-regulated kinase 2 mitogen-actived protein kinase phosphorylation and regulation of activity of PDE4 cyclic adenosine monophosphate-specific phosphodiesterases / Elaine V. Hills, Miles D. Houslay, and George S. Baillie -- Radiolabeled ligand binding to the catalytic or allosteric sites of PDE5 and PDE11 / James L. Weeks ... [et al.] -- Analysis of dimerization determinants of the PDE6 catalytic subunits / Khakim G. Muradov, Kimberley K. Boyd, and Nikolai O. Artemyev -- Interaction between catalytic and inhibitory subunits of PDE6 / Nikolai O. Artemyev -- Purification, reconstitution on lipid vesciles and assays of PDE6 and its activator G protein, transducin -- Theodore G. Wensel, Feng He, and Justine A. Malinski. Study of cyclic adenosine monophosphate microdomains in cells / Marco Mongillo ... [et al.] -- High-resolution measurements of cyclic adenosine monophosphate signals in 3D microdomains / Jeffrey W. Karpen and Thomas C. Rich -- Cygnets : in vivo characterization of novel cGMP indicators and in vivo imaging of intracellular cGMP / Akira Honda ... [et al.] -- High-throughput screening of phosphodiesterase activity in living cells / Thomas C. Rich and Jeffrey W. Karpen -- Assessment of phosphodiesterase isozyme contribution in cell and tissue extracts / Thèrése Keravis, Rima Thaseldar-Roumié, and Claire Lugnier -- Localization of the cyclic guanosine 3', 5'-monophosphate-hydrolysing phosphodiesterase type 9 in rat brain by nonradioactive in situ hybridization / Wilma C.G. Van Staveren and Marjanne Markerink-Van Ittersum -- Determination of Ca2+/calmodulin-stimulated phosphodiesterase activity in intact cells / Chen Yan -- Adenovirus-mediated overexpression of murine cyclic nucleotide phosphodiesterase 3B / Faiyaz Ahmad ... [et al.] -- Identification of promoter elements in the 5'-flanking region of the murine cyclic nucleotide phosphodiesterase 3B gene / Hanguan Liu ... [et al.] --
  • 2011 Springer Protocols
    edited by James J. Chambers, Richard H. Kramer.
    Introduction to part I : caged neurotransmitters / James J. Chambers and Richard H. Kramer -- Targeting and excitation of photoactivatable molecules : design considerations for neurophysiology experiments / Eugene F. Civillico, J. Peter Rickgauer, and Samuel S.-H Wang -- Are caged compounds still useful / Graham C.R. Ellis-Davis -- Chromophores for the delivery of bioactive molecules with two-photon excitation / Timothy M. Dore and Hunter C. Wilson -- Introduction to part II : natural photosensitive proteins / James J. Chambers and Richard H. Kramer -- Light-activated ion pumps and channels for temporally precise optical control of activity in genetically targeted neurons / Brian Y. Chow [and others] -- Vertebrate and invertebrate rhodopsins : light control of G-protein signaling / Davina V. Gutierrez, Eugene Oh, and Stefan Herlitze -- Restoring visual function after photoreceptor degeneration : ectopic expression of photosensitive proteins in retinal neurons / Bin Lin and Richard H. Masland -- Introduction to part III : small molecule photoswitches / James J. Chambers and Richard H. Kramer -- Photoswitch design / Doris L. Fortin and Richard H. Kramer -- Optical manipulation of protein activity and protein interactions using caged proteins and optical switch protein conjugates / Yuling Yan and Gerard Marriott -- Structure-based design of light-controlled proteins / Harald Janovjak and Ehud Y. Isacoff -- Photoswitchable ligand-gated ion channels / Pau Gorostiza and Ehud Y. Isacoff.
  • 2008 Springer Protocols
    edited by William J. Murphy.
    From gene-scale to genome-scale phylogenetics : the data flood in, but the challenges remain / Antonis Rokas and Stylianos Chatzimanolis -- Phylogenomic analysis of chromosome sorting and painting / Roscoe Stanyon and Gary Stone -- FISH for mapping single copy genes / Terje Raudsepp and Bhanu P. Chowdhary -- Construction of radiation hybrid panels / John E. Page and William J. Murphy -- Survey sequencing and radiation hybrid mapping to construct comparative maps / Christophe Hitte ... [et al.] -- Construction of high-resolution comparative maps in mammals using BAC-end sequences / Denis M. Larkin and Harris A. Lewin -- Amniote phylogenomics : testing evolutionary hypotheses with BAC library scanning and targeted clone analysis of large-scale sequences from reptiles / Andrew M. Shedlock, Daniel E. Janes, and Scott V. Edwards -- Comparative physical mapping : universal overgo hybridization probe design and BAC library hybridization / James W. Thomas -- Phylogenomic resources at the UCSC Genome Browser / Kate Rosenbloom ... [et al.] -- Computational tools for the analysis of rearrangements in mammalian genomes / Glenn Tesler and Guillaume Bourque -- Computational reconstruction of ancestral DNA sequences / Mathieu Blanchette ... [et al.] -- Sequencing and phylogenomic analysis of whole mitochondrial genomes of animals / Rafael Zardoya and Mónica Suárez -- Retroposons : genetic footrpints on the evolutionary paths of life / Hidenori Nishihara and Norihiro Okada -- LINE-1 elements : analysis by FISH and nucleotide sequences / Paul D. Waters ... [et al.] -- Identification of cryptic sex chromosomes and isolation of X- and Y-borne genes / Paul D. Waters ... [et al.]
  • 2011 Springer
    Peter R. Bergethon, Kevin Hallock.
    pt. 1. Principles of biophysical inquiry -- pt. 2. Foundations -- pt. 3. Building a model of biomolecular structure -- pt. 4. Function and action biological state space -- pt. 5. Methods for the measuring structure and function -- pt. 6. Physical constants.
  • 2015 Wiley
    Gordon G. Hammes, Sharon Hammes-Schiffer.
    Chapter 1. Heat, Work, and Energy (pages 1-21) -- Chapter 2. Entropy and Gibbs Energy (pages 23-42) -- Chapter 3. Applications of Thermodynamics to Biological Systems (pages 43-76) -- Chapter 4. Thermodynamics Revisited (pages 77-94) -- Chapter 5. Principles of Chemical Kinetics (pages 95-120) -- Chapter 6. Applications of Kinetics to Biological Systems (pages 121-152) -- Chapter 7. Fundamentals of Quantum Mechanics (pages 153-176) -- Chapter 8. Electronic Structure of Atoms and Molecules (pages 177-202) -- Chapter 9. X-ray Crystallography (pages 203-224) -- Chapter 10. Electronic Spectra (pages 225-252) -- Chapter 11. Circular Dichroism, Optical Rotary Dispersion, and Fluorescence Polarization (pages 253-276) -- Chapter 12. Vibrations in Macromolecules (pages 277-288) -- Chapter 13. Principles of Nuclear Magnetic Resonance and Electron Spin Resonance (pages 289-314) -- Chapter 14. Applications of Magnetic Resonance to Biology (pages 315-330) -- Chapter 15. Fundamentals of Statistical Mechanics (pages 331-356) -- Chapter 16. Molecular Simulations (pages 357-382) -- Chapter 17. Ligand Binding to Macromolecules (pages 383-414) -- Chapter 18. Hydrodynamics of Macromolecules (pages 415-440) -- Chapter 19. Mass Spectrometry (pages 441-456).
    Also available: Print – 2015
  • 2007
    Gordon G. Hammes.
    Approachable, yet thorough, this book presents physical chemistry in conceptual terms with a minimum of mathematics. Providing the basic knowledge and tools that every biologist should have to understand the quantitative interpretation of biological phenomena, it covers: Fundamentals of thermodynamics and chemical kinetics ; Fundamentals of spectroscopy and structure determination ; Ligand binding to macromolecules, hydrodynamics, and mass spectrometry. All techniques and concepts are clearly illustrated with relevant applications and examples from the biological sciences. Problems at the end of each chapter reinforce the principles.
  • 2009 ScienceDirect
    [edited by] F. Javier Alvarez-Leefmans and Eric Delpire.
    Part I. Overview of Chloride Transporters and Channels -- Part II. Current Methods for Studying Chloride Regulation -- Part III. From Cloning to Structure, Function and Regulation of Chloride Transporters and Channels -- Part IV. Cation-Chloride Cotransporters in Neural Function and Dysfunction -- Part V. Cation-Chloride Cotransport in Choroid Plexus and Blood-Brain Barrier.
  • 2014 Springer Protocols
    edited by Mikiko C. Siomi.
    Chromatin Immunoprecipitation Assay of Piwi in Drosophila / Hang Yin and Haifan Lin -- Drosophila Germline Stem Cells for In Vitro Analyses of PIWI-Mediated RNAi / Yuzo Niki, Takuya Sato, Takafumi Yamaguchi, Ayaka Saisho, Hiroshi Uetake, and Hidenori Watanabe -- RNAi and Overexpression of Genes in Ovarian Somatic Cells / Kuniaki Saito -- Making piRNAs In Vitro / Shinpei Kawaoka, Susumu Katsuma, and Yukihide Tomari -- A Framework for piRNA Cluster Manipulation / Ivan Olovnikov, Adrien Le Thomas, and Alexei A. Aravin -- Biochemical and Mass Spectrometric Analysis of 3ʹ-End Methylation of piRNAs / Takeo Suzuki, Kenjyo Miyauchi, Yuriko Sakaguchi, and Tsutomu Suzuki -- HITS-CLIP (CLIP-Seq) for Mouse Piwi Proteins / Anastassios Vourekas and Zissimos Mourelatos -- DNA Methylation in Mouse Testes / Satomi Kuramochi-Miyagawa, Kanako Kita-Kojima, Yusuke Shiromoto, Daisuke Ito, Hirotaka Koshima, and Toru Nakano -- Analysis of Small RNA-Guided Endonuclease Activity in Endogenous Piwi Protein Complexes from Mouse Testes / Michael Reuter and Ramesh S. Pillai -- Small RNA Library Construction from Minute Biological Samples / Jessica A. Matts, Yuliya Sytnikova, Gung-wei Chirn, Gabor L. Igloi, and Nelson C. Lau -- Analysis of sDMA Modifications of PIWI Proteins / Shozo Honda, Yoriko Kirino, and Yohei Kirino -- Analyses of piRNA-Mediated Transcriptional Transposon Silencing in Drosophila: Nuclear Run-On Assay on Ovaries / Sergey Shpiz and Alla Kalmykova -- Combined RNA/DNA Fluorescence In Situ Hybridization on Whole-Mount Drosophila Ovaries / Shpiz, Sergey Lavrov, and Alla Kalmykova -- Fast and Accurate Method to Purify Small Noncoding RNAs from Drosophila Ovaries / Thomas Grentzinger and Séverine Chambeyron -- Isolation of Zebrafish Gonads for RNA Isolation / Hsin-Yi Huang and René F. Ketting -- Small RNA Library Construction for High-Throughput Sequencing / Jon McGinn and Benjamin Czech -- Analysis of Piwi-Loaded Small RNAs in Terahymena / Tomoko Noto, Henriette M. Kurth, and Kazufumi Mochizuki -- Effective Gene Knockdown in the Drosophila Germline by Artificial miRNA-Mimicking siRNAs / Hailong Wang, Haidong Huang, and Dahua Chen -- Isolation and Bioinformatic Analyses of Small RNAs Interacting with Germ Cell-Specific Argonaute in Rice / Reina Komiya and Ken-Ichi Nonomura.
  • Springer Protocols
    v. 2 Springer Protocols
    edited by Michael J. Soares and Joan S. Hunt.
  • 2015 Springer Protocols
    edited by José M. Estevez.
    Penium margaritaceum as a model organism for cell wall analysis of expanding plant cells / Maja G. Rydahl ... [et al.] -- Using chemical genomics to study cell wall formation and cell growth in Arabidopsis thaliana and Penium margaritaceum / N. Worden ... [et al.] -- Optimized method for growing in vitro Arabidopsis thaliana pollen tubes / Cecilia Borassi ... [et al.] -- Imaging of calcium dynamics in pollen tube cytoplasm / María Laura Barberini and Jorge Muschietti -- Live imaging of root hairs / Silvia M. Velasquez, Jose R. Dinneny, and José M. Estevez -- Improved ROS measurement in root hair cells / Paola Silvina Denita Juárez, Silvina Mangano, and José M. Estevez -- Root hair assay to expedite cell death research / Joanna Kacprzyk and Paul F. McCabe -- Vacuolar staining methods in plant cells / David Scheuring ... [et al.] -- Live cell imaging of FM4-64, a tool for tracing the endocytic pathways in Arabidopsis root cells / Adeline Rigal, Siamsa M. Doyle, and Stéphanie Robert -- Salt-stress regulation of root system growth and architecture in Arabidopsis seedlings / Lina Duan, Jose Sebastian, and Jose R. Dinneny -- Quantification of fluorescent reporters in plant cells / Michael Pound, Andrew P. French, and Darren M. Wells -- Live cell imaging of the cytoskeleton and cell wall enzymes in plant cells / Arun Sampathkumar and Raymond Wightman -- Using the split-ubiquitin yeast two-hybrid system to test protein-protein interactions of transmembrane proteins / Logan Bashline and Ying Gu -- Activation tag screening for cell expansion genes in Arabidopsis thaliana / Chaowen Xiao and Charles T. Anderson -- BiFC for protein-protein interactions and protein topology : discussing an integrative approach for an old technique / Giovanni Stefano, Luciana Renna, and Federica Brandizzi -- N-Glycosylation and plant cell growth / Christiane Veit, Ulrike Vavra, and Richard Strasser -- Peptide separation methodologies for in-depth proteomics / Sajad Majeed Zargar ... [et al.] -- Structural and mechanical characterization of growing Arabidopsis plant cell walls / Friederike Saxe, Ingo Burgert, and Michaela Eder.
  • 2013 Springer Protocols
    edited by Teun Munnick, Ingo Heilmann.
    As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.
  • ScienceDirect
    edited by Arthur Weissbach, Herbert Weissbach.
    Also available: Print – 1986
  • 2013 Springer Protocols
    edited by Ive De Smet.
    The tomato leaf as a model system for organogenesis -- Usefulness of Physcomitrella patens for studying plant organogenesis -- The dicot root as a model system for studying organogenesis -- Genetic control of root organogenesis in cereals -- Gene expression analysis of aquatic angiosperms podostemaceae to gain insight into the evolution of their enigmatic morphology -- Brown algae as a model for plant organogenesis -- Microscopic analysis of ovule development in Arabidopsis thaliana -- Imaging of phenotypes, gene expression, and protein localization during embryonic root formation in Arabidopsis -- Inducible system for lateral roots in Arabidopsis thaliana and maize -- Adventitious root induction in Arabidopsis thaliana as a model for in vitro root organogenesis -- High-throughput, noninvasive imaging of root systems -- Small-molecule screens to study lateral root development -- Cell lineage analyses in living tissues -- Protein immunolocalization in maize tissues -- Auxin immunolocalization in plant tissues -- Gene expression analysis of shoot apical meristem cell types -- Kinematic analysis of cell division in leaves of mono- and dicotyledonous species: A basis for understanding growth and developing refined molecular sampling strategies -- Regeneration in arabidopsis tissue culture -- Isolation and analysis of mRNAs from specific cell types of plants by ribosome immunopurification -- Analyzing small and long RNAs in plant development using non-radioactive in situ hybridization -- Analyzing protein distribution in plant tissues using "Whole-mount" Immunolocalization -- Culture methods and mutant generation in the filamentous brown algae Ectocarpus siliculosus -- Building simulation models of developing plant organs using VirtualLeaf.
  • 2016 Springer Protocols
    edited by Jose R. Botella, Miguel A. Botella.
    Suppressor screens in Arabidopsis / Xin Li and Yuelin Zhang -- Quantitative reverse transcription-qPCR-based gene expression analysis in plants / Heithem Ben Abdallah and Petra Bauer -- DNA-binding factor target identification by chromatin immunoprecipitation (ChIP) in plants / David Posé and Levi Yant -- Identification of genes responsible for natural variation in volatile content using next-generation sequencing technology / Iraida Amaya, Jeremy Pillet, and Kevin M. Folta -- Micro-tom tomato as an alternative plant model system : mutant collection and efficient transformation / Masahito Shikata and Hiroshi Ezura -- Culture of the tomato micro-tom cultivar in greenhouse / Christophe Rotha ... [et al.] -- Virus-induced gene silencing as a tool to study tomato fruit biochemistry / Elio Fantini and Giovanni Giuliano -- Simplified and rapid method for the isolation and transfection of Arabidopsis leaf mesophyll protoplasts for large-scale applications / Arnaldo L. Schapire and L. Maria Lois -- Preparation of epidermal peels and guard cell protoplasts for cellular, electrophysiological, and -omics assays of guard cell function / Mengmeng Zhu ... [et al.] -- Application of variable angle total internal reflection fluorescence microscopy to investigate protein dynamics in intact plant cells / Yinglang Wan ... [et al.] -- Immunoprecipitation of plasma membrane receptor-like kinases for identification of phosphorylation sites and associated proteins / asuhiro Kadota, Alberto P. Macho, and Cyril Zipfel -- Yeast three-hybrid system for the detection of protein-protein interactions / Natsumi Maruta, Yuri Trusov, and Jose R. Botella -- Cautions in measuring in vivo interactions using FRET and BiFC in Nicotiana benthamiana / Meral Tunc-Ozdemir, Yan Fu, and Alan M. Jones -- Analysis of protein-lipid interactions using purified C2 domains / Jessica Pérez-Sancho ... [et al.] -- Assessing kinase activity in plants with in-gel kinase assays / Pengcheng Wang and Jian-Kang Zhu -- Analyses of plant UDP-dependent glycosyltransferases to identify their volatile substrates using recombinant proteins / Yusuke Kamiyoshihara, Denise M. Tieman, and Harry J. Klee -- Competitive index : mixed infection-based virulence assays for genetic analysis in Pseudomonas syringae-plant interactions / Alberto P. Macho ... [e tal.] -- Simplified assays for evaluation of resistance to Alternaria brassicicola and turnip mosaic virus / Yuri Trusov ... [et al.] -- Simultaneous determination of plant hormones by GC-TOF-MS / José G. Vallarino and Sonia Osorio.
  • 2008 Springer Protocols
    editors: Gary D. Foster, I. Elisabeth Johansen, Yiguo Hong, Peter D. Nagy.
  • 2014 Springer Protocols
    edited by Paul Birch, John T. Jones, Jorunn I.B. Bos.
    Galaxy as a Platform for Identifying Candidate Pathogen Effectors -- Bioinformatic analysis of expression data to identify effector candidates -- Two-dimensional data binning for the analysis of genome architecture in filamentous plant pathogens and other eukaryotes -- On The Statistics Of Identifying Candidate Pathogen Effectors -- High-throughput imaging of plant immune responses -- In vivo protein-protein interaction studies with BiFC: conditions, cautions and caveats -- Particle bombardment-mediated transient expression to identify localization signals in plant disease resistance proteins and target sites for the proteolytic activity of pathogen effectors -- Purification of fungal haustoria from infected plant tissue by flow cytometry -- Functional Characterisation of Nematode Effectors in Plants -- Silencing of aphid genes by feeding on stable transgenic Arabidopsis thaliana -- Leaf-disc assay based on transient over-expression in Nicotiana benthamiana to allow functional screening of candidate effectors from aphids -- A growth quantification assay for Hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana -- Simple quantification of in planta fungal biomass -- Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum -- DIGE-ABPP by click chemistry: Pairwise comparison of serine hydrolase activities from the apoplast of infected plants -- A Simple and Fast Protocol for the Protein Complex Immunoprecipitation (Co-IP) of Effector Host Protein Complexes -- An Arabidopsis and Tomato Mesophyll Protoplast System for fast Identification of early MAMP-triggered Immunity-Suppressing Effectors -- Production of RXLR effector proteins for structural analysis by X-ray crystallography -- The Dos and Donts of effectoromics -- Protoplast cell death assay to study Magnaporthe oryzae AVR gene function in rice -- A Bacterial Type III Secretion Based Delivery System for Functional Assays of Fungal Effectors in Cereals -- Capture arrays to annotate resistance genes in plant genomes and to accelerate plant resistance gene discovery.
  • 2014 Springer Protocols
    edited by Joanna Rorbach, Agnieszka J. Bobrowicz.
  • 2002 Springer Protocols
    edited by Christoph Kannicht.
    Assignment of disulfide bonds in proteins by chemical cleavage and peptide mapping by mass spectrometry / Jiang Wu -- Carbohydrate composition analysis of glycoproteins using highly sensitive fluorescence detection methods / George N. Saddic -- Enzymatical hydrolysis of N-glycans from glycoproteins and fluorescent labeling by 2-aminobenzamide (2-AB) / Rolf Nuck -- Separation of N-glycans by HPLC / Martin Gohlke -- Enzymatic sequence analysis of N-glycans / Christoph Kannicht -- Immunological detection of O-GlcNAc / Monika Rex-Mathes -- Analysis of O-glycosylation / Juan J. Calvete -- Characterization of site-specific glycosylation / Katalin F. Medzihradszky -- Monitoring glycosylation of therapeutic glycoproteins for consistency using highly fluorescent anthranilic acid / Shirish T. Dhume -- Metabolic labelling and structural analysis of glycosylphosphatidylinositols from parasitic protozoa / Peter Gerold -- Analysis of S-acylation of proteins / Michael Veit -- Immunoblotting methods for the study of protein ubiquitination / Edward G. Mimnaugh -- Analysis of methylation and acetylation in E. coli ribosomal proteins / Randy J. Arnold -- Identification of in vivo protein phosphorylation sites with mass spectrometry / Jun Qin -- Analysis of tyrosine-O-sulfation / Jens R. Bundgaard -- a-amidated peptides: approaches for analysis / Gregory P. Mueller -- y-glutamate and B-hydroxyaspartate in proteins / Francis J. Castellino -- Detection of isoAspartate residues as a posttranslational modification of proteins and peptides / Verne Schirch -- Lysine hydroxylation and crosslinking of collagen / Mitsuo Yamauchi -- Heterologous expression in endocrine cells for analysis of posttranslational modifications / Jens R. Bundgaard -- 2D-electrophoresis: detection of glycosylation and influence on spot pattern / Klemens Löster.
  • 2015 Springer
    Ashraf Khan, Ian O. Ellis, Andrew M. Hanby, Ediz F. Cosar, Emad A. Rakha, Dina Kandil, editors.
    Molecular basis of breast cancer imaging -- Familial Breast Cancer and Genetic Predisposition in Breast Cancer -- Modelling the Molecular Pathology of Breast Cancer Initiation -- Molecular Pathology of Precancerous Lesions of the Breast -- Breast Cancer Stem Cells: Role in Tumor Initiation, Progression and Targeted Therapy -- Molecular Pathology of Pre-Invasive Ductal Carcinoma. -Molecular Pathology of Lobular Carcinoma Ali Sakhdari, Lloyd Hutchinson, Ediz Cosar -- Molecular Pathology of Hormone Regulation in Breast Cancer: Hormone Receptor Evaluation and Therapeutic Implications -- Molecular Pathology of HER Family of Oncogenes in Breast Cancer: HER-2 Evaluation and Role in Targeted Therapy -- Molecular Classification of Breast Cancer -- Triple Negative Breast Cancer: Subtypes with Clinical Implications -- Molecular-based Diagnostic, Prognostic and Predictive Tests in Breast Cancer -- Role of MicroRNAs in Breast Cancer -- Molecular Pathology of Fibroepithelial Neoplasms of the Breast -- Molecular Features of Mesenchymal Tumors of the Breast -- Molecular Pathology of Breast Cancer Metastasis -- The molecular pathology of chemoresistance during the therapeutic response in breast cancer -- The Molecular Pathology of Male Breast Cancer -- Specimens for Molecular Testing in Breast Cancer.
  • Casey, Denise.
    This is an introduction to the science behind the human genome project and was prepared for the Department of Energy's (DOE) Human Genome Program Report for 1991-92. This document contains information on the biology of DNA, techniques for sequencing and mapping genes, and genome informatics.
  • 2008 Springer
    Elizabeth van Pelt-Verkuil, Alex van Belkum, John P. Hays.
  • 2012 ClinicalKey
    Gerhard Meisenberg, William H. Simmons.
    Drs. Gerhard Meisenberg and William H. Simmons covers the latest developments in the field, including genome research, the molecular basis of genetic diseases, techniques of DNA sequencing and molecular diagnosis, and more. An updated and expanded collection of figures and access to the fully searchable text, USMLE test questions, clinical case studies and more makes this the resource for understanding all aspects of biochemistry needed in medicine. Tap into the most up-to-date coverage of new developments in genome research, the molecular basis of genetic diseases, techniques of DNA sequencing and molecular diagnosis, RNA interference as a mechanism both for regulation of gene expression and for anti-viral defense, and more. Gain a clear visual understanding through new and updated figures that provide current and relevant guidance. Make the link between basic science and clinical medicine with new Clinical Example boxes in nearly every chapter. Focus on the most essential biochemistry principles and how they apply to clinical medicine.
  • 2013 Ovid
    editors, Dongfeng Tan, Henry T. Lynch.
    Section I. Introduction of cancer biology and cancer genetics -- Section II. Molecular techniques -- Section III. Molecular tests and cancer management -- Section IV. Molecular diagnostics of common malignancies -- Section V. Molecular basis of cancer therapeutics -- Section VI. Personalized medicine and targeted therapy of cancer -- Section VII. Future of molecular diagnostics and personalized cancer medicine.
  • 2006 Springer
    edited by Marschall S. Runge, Cam Patterson ; foreword by Victor A. McKusick.
    Also available: Print – 2006
  • v. 1-2, 2009.
    S.J. Flint, V.R. Racaniello, L.W. Enquist, A.M. Skalka.
    Status: Not Checked OutLane Catalog Record
    v. 1. Molecular biology -- v. 2. Pathogenesis and control. v. 1. Foundations -- The infectious cycle -- Genomes and genetics -- Structure -- Attachment and entry -- Synthesis of RNA from RNA templates -- Reverse transcription and integration -- Transcription strategies : DNA templates -- Genome replication strategies : DNA viruses -- Processing of viral pre-mRNA -- Control of translation -- Intracellular trafficking -- Assembly, exit, and maturation -- v. 2. Infection of a susceptible host -- Infection of populations -- Virus offense meets host defense : early actions -- Immune defenses -- Patterns of infection -- Human immunodeficiency virus pathogenesis -- Transformation and oncogenesis -- Vaccines -- Antiviral drugs -- Evolution and emergence.
  • 2006 Springer Protocols
    edited by Franck Pellestor.
  • 2008 Springer Protocols
    Andrew F. Hill, editor.
    Cell culture models to unravel prion protein function and aberrancies in prion diseases / Katarina Bedecs -- Investigation of PrPc metabolism and function in live cells : methods for studying individual cells and cell populations / Cathryn L. Haigh and David R. Brown -- Immunodetection of PrPsc using western and slot blotting techniques / Hanny Gyllberg and Kajsa Löfgren -- Assaying prions in cell culture : the standard scrapie cell assay (SSCA) and the scrapie cell assay in end point format (SCEPA) / Sukhvir P. Mahal ... [et al.] -- Generation of cell lines propagating infectious prions and the isolation and characterization of cell-derived exosomes / Laura J. Vella and Andrew F. Hill -- Neurotoxicity of prion peptides on cultured cerebellar neurons / Giuseppe D. Ciccotosto, Roberto Cappai, and Anthony R. White -- Understanding the nature of prion diseases using cell-free assays / Victoria A. Lawson -- Methods for conversion of prion protein into amyloid fibrils / Leonid Breydo, Natallia Makarava, and Ilia V. Baskakov -- Amplification of purified prions in vitro / Surachai Supattapone, Nathan R. Deleault, and Judy R. Rees -- Expression and purification of full-length recombinant PrP of high purity / Natallia Makarava and Ilia V. Baskakov -- Analysis of PrP conformation using circular dichroism / Sen Han and Andrew F. Hill -- Effect of copper on the de novo generation of prion protein expressed in Pichia pastoris / Carina Treiber -- Biophysical investigations of the prion protein using electron paramagnetic resonance / Simon C. Drew and Kevin J. Barnham -- Molecular diagnosis of human prion disease / Jonathan D.F. Wadsworth ... [et al.] -- Analysis of endogenous PrPc processing in neuronal and non-neuronal cell lines / Victoria Lewis and Steven J. Collins -- Molecular typing of PrPres in human sporadic CJD brain tissue / Victoria Lewis ... [et al.] -- Transgenic mouse models of prion diseases / Glenn C. Telling -- Quantitiative bioassay of surface-bound prion infectivity / Victoria A. Lawson.
  • Lawrence E. Hunter.
    In the beginning -- Evolution -- A little bit of chemistry -- The structure and function of bacteria -- Biological macromolecules -- Eukaryotes -- Multicellular organisms and development -- Anatomy, physiology, and systems biology -- Disease and its treatment -- Molecular biotechnology -- Molecular bioethics.
  • 2013 Springer
    Kenn Gerdes, editor.
    Introduction -- Type I Toxin - Antitoxin Systems: Hok/Sok and Fst -- Novel Type I Toxin - Antitoxin Systems -- Type II TA Loci: The Ccdab and Parde Families -- Type II TA Loci: The Relbe Family -- Type II TA Loci: The Unusual Mqsra Locus -- Type II TA Loci: The Mazef Family -- Type II TA Loci: Vapbc and Other TA Loci In Mycobacteria -- Type II TA Loci: Phd Doc Family -- Type II TA Loci: The Fic Family -- Type II TA Loci, Hipab And Persisters -- Type II TA Loci: Zeta/Pezt Family -- Type II Loci: Phylogeny -- Type III TA Loci -- TA Loci Encoded By Plasmids -- TA Loci in Archaea -- TA Loci in Mycobacterium Tuberculosis -- TA Loci in Streptococcus Pneumoniae -- Biotechnological and Medical Exploitations Of TA Genes and Their Components.
  • 2011 Springer Protocols
    edited by Majambu Mbikay, Nabil G. Seidah.
    On the Discovery of Precursor Processing -- The Prohormone Theory and the Proprotein Convertases: It Is All About Serendipity -- The Proprotein Convertases, 20 Years Later -- Insights from Bacterial Subtilases into the Mechanisms of Intramolecular Chaperone-Mediated Activation of Furin -- The Novel Role of Cathepsin L for Neuropeptide Production Illustrated by Research Strategies in Chemical Biology with Protease Gene Knockout and Expression -- In Vitro Assay for Protease Activity of Proprotein Convertase Subtilisin Kexins (PCSKs): An Overall Review of Existing and New Methodologies -- Inhibitor Screening of Proprotein Convertases Using Positional Scanning Libraries -- Analyses of PCSK9 Post-Translational Modifications Using Time-of-Flight Mass Spectrometry -- The Molecular Biology of Furin-Like Proprotein Convertases in Vascular Remodelling -- Identification of the Myosin Heavy Polypeptide 9 as a Downstream Effector of the Proprotein Convertases in the Human Colon Carcinoma HT-29 Cells -- Regulation of 7B2 mRNA Translation: Dissecting the Role of Its 5'-Untranslated Region -- Analysis of Epigenetic Alterations to Proprotein Convertase Genes in Disease -- Genetic and Functional Characterization of PCSK1 -- Prohormone Processing in Zebrafish -- Use of Zebrafish and Knockdown Technology to Define Proprotein Convertase Activity -- Characterization of Impaired Processing of Neuropeptides in the Brains of Endoproteases Knockout Mice -- Quantitative Peptidomics of Mice Lacking Peptide-Processing Enzymes -- A Proteomic Protocol to Identify Physiological Substrates of Proprotein Convertases -- Neurophenotyping Genetically-Modified Mice for Social Behavior.
  • 2010
    Lihan Sun.
    The nearly 9 million incidences of myocardial infarction (MI) and the resultant heart failure in the United States alone call for a continued need for the development of novel therapeutics. However, the molecular basis of these diseases is highly complex and awaits further elucidation. Our lab has a long-standing interest in determining the role of protein kinase C (PKC) in cardiovascular diseases. Elevated expression of alpha, beta, epsilon and deltaPKCs is associated with cardiac dysfunction in failing murine and human hearts. Moreover, genetic and pharmacological modulations of PKC activities exert profound impact on the state of these diseases. However, characterization of the specific role of each PKC isozyme was hampered by the lack of isozyme-selective PKC regulators. To this end, Mochly-Rosen and co-workers have rationally designed and developed isozyme-selective peptide activators and inhibitors of PKC that have enabled us to examine the role of each PKC isozyme in any biological system. Using an epsilonPKC-selective inhibitor, we found that sustained inhibition of epsilonPKC suppressed excessive cardiac fibrosis and preserved cardiac function in a rat model of hypertension-induced heart failure, in vivo. To uncover the mechanistic link between epsilonPKC and cardiac fibrosis, I showed that activation of epsilonPKC increased the activity matrix metalloproteinase-2 (MMP-2), a critical protease that degrades the extracellular matrix to facilitate cardiac fibrosis. Further, using the epsilonPKC inhibitor and cardiac fibroblasts that lack epsilonPKC, I discovered that histone deacetylase 6 (HDAC6), a cytosolic enzyme that deaceylates alpha-tubulin, participates in epsilonPKC modulation of MMP-2 activity during cardiac fibrosis. These results suggest that the epsilonPKC-selective inhibitor can be a potential therapeutic for the treatment of heart failure. Aldehyde dehydrogenase 2 (ALDH2) was identified by our group as a mitochondrial substrate for epsilonPKC that confers cardioprotection from ischemic injury. ALDH2 activation during MI is cardioprotective, because it oxidizes and thus removes toxic aldehydes that accumulate due to lipid peroxidation during ischemia-reperfusion. Since these aldehydes form protein adducts that inactive many cellular enzymes, they contribute to non-reversible damage to the ischemic myocardium. A recent study discovered that ALDH2 is required for nitroglycerin bioactivation, in vivo, but its enzymatic activity is inhibited by prolonged treatment with nitroglycerin. This has prompted us to determine potential risks associated with nitroglycerin tolerance in association with the role that ALDH2 has in cardiac protection. The clinical implications here are of special interest to us, because any risks pertinent to nitroglycerin treatment, if validated, can affect millions of patients with cardiac diseases. Therefore, I set off to determine whether sustained nitroglycerin treatment increases injury to the ischemic myocardium using a rat model of myocardial infarction (MI). I confirmed that sustained nitroglycerin treatment, as might be given to patients with angina pectoris, inhibited ALDH2 activity in the rat myocardium, in vivo. Further, I found that continuous treatment of nitroglycerin resulted in more than 2-fold increase in infarct size and worsened cardiac function after MI, in vivo. I then used Alda-1, a selective ALDH2 activator our group discovered, to assess whether activation of ALDH2 could undo the nitroglycerin-induced cardiac injury. Indeed, concomitant treatment with Alda-1 and nitroglycerin inhibited nitroglycerin-induced increase in infarct size and restored post-MI cardiac function. This finding poses a challenge to the prevalent clinical practice: patients, under sustained nitroglycerin therapy for the management of angina pectoris, are at risk of increased cardiac damage if they develop an MI during the nitroglycerin treatment. My animal studies suggest that if Alda-1 is given together with nitroglycerin, it can reduce cardiac damage caused by nitroglycerin tolerance. Finally, to determine the mechanism underlying the role of nitroglycerin and Alda-1 in MI injury, I demonstrated that Alda-1 prevented nitroglycerin-induced inactivation of ALDH2 and the resultant elevation of protein carbonylation, a hallmark of toxic aldehydic adducts, in the myocardium. The result substantiates a health mechanism for the heart: loss of control over oxidative stress leads to an injured myocardium and a simple correction of that function undoes the bad.
  • 2011 Springer Protocols
    edited by Dermot Walls and Sinéad T. Loughran.
    A digest of protein purification -- Gel-filtration chromatography -- Immunoaffinity chromatography -- Avoiding proteolysis during protein chromatography -- Scale-up of protein purification : Downstream processing issues -- Phage display : A powerful technology for the generation of high specificity affinity reagents from alternative immune sources -- Engineering protein stability -- Microfluidics in protein chromatography -- Tagging recombinant proteins to enhance solubility and aid purification -- Storage and lyophilisation of pure proteins -- Differential precipitation and solubilization of proteins -- Ion-exchange chromatography : Basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase -- Protein quantitation and analysis of purity -- Purification of proteins fused to glutathione S-transferase -- Purification of proteins fused to maltose-binding protein -- Purification of proteins from baculovirus-infected insect cells -- Purification of poly-histidine-tagged proteins -- Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by cid/etd tandem mass spectrometry -- Tag removal by site-specific cleavage of recombinant fusion proteins -- Purification of antibodies using affinity chromatography -- Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens -- Measuring protein-protein interactions using biacore -- Ultra-performance liquid chromatography-mass spectrometry of proteins -- Hydrophobic interaction chromatography -- Fast protein liquid chromatography -- Shotgun proteomics : A qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS -- Shotgun proteomics : A relative quantitative approach using off-gel electrophoresis and LC-MS/MS -- Clinical proteomics : Liquid chromatography-mass spectrometry purification systems -- Strategies for the purification of membrane proteins -- A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases.
  • 2014 Springer
    Ke-li Han, Xin Zhang, Ming-jun Yang, editors.
    "This book discusses how biological molecules exert their function and regulate biological processes, with a clear focus on how conformational dynamics of proteins are critical in this respect. In the last decade, the advancements in computational biology, nuclear magnetic resonance including paramagnetic relaxation enhancement, and fluorescence-based ensemble/single-molecule techniques have shown that biological molecules (proteins, DNAs and RNAs) fluctuate under equilibrium conditions. The conformational and energetic spaces that these fluctuations explore likely contain active conformations that are critical for their function. More interestingly, these fluctuations can respond actively to external cues, which introduces layers of tight regulation on the biological processes that they dictate. A growing number of studies have suggested that conformational dynamics of proteins govern their role in regulating biological functions, examples of this regulation can be found in signal transduction, molecular recognition, apoptosis, protein / ion / other molecules translocation and gene expression"--Publisher's description.
    Also available: Print – 2014
  • 2014 Springer Protocols
    edited by Valentin Köhler.
    De novo design of stable [alpha]-helices / Alexander Yakimov, Georgy Rychkov, and Michael Petukhov -- Design of monomeric water-soluble [Beta]-hairpin and [Beta]-sheet peptides/ M. Angeles Jiménez -- Combination of theoretical and experimental approaches for the design and study of fibril-forming peptides / Phanourios Tamamis ... [et al.] -- Posttranslational incorporation of noncanonical amino acids in the RNase S system by semisynthetic protein assembly / Maika Genz and Norbert Sträter -- Design, synthesis, and study of fluorinated proteins / Benjamin C. Buer and E. Neil G. Marsh -- High-quality combinatorial protein libraries using the binary patterning approach / Luke H. Bradley -- Methods for library-scale computational protein design / Lucas B. Johnson, Thaddaus R. Huber, and Christopher D. Snow -- Symmetric protein architecture in protein design : top-down symmetric deconstruction / Liam M. Longo and Michael Blaber -- Identification of protein scaffolds for enzyme design using scaffold selection / André C. Stiel, Kaspar Feldmeier, and Birte Höcker -- Computational design of novel enzymes without cofactors / Matthew D. Smith, Alexandre Zanghellini, and Daniela Grabs-Röthlisberger -- De novo design of peptide scaffolds as novel preorganized ligands for metal-ion coordination / Aimee J. Gamble and Anna F. A. Peacock -- Computational design of metalloproteins / Avanish S. Parmar, Douglas Pike, and Vikas Nanda -- Incorporation of modified and artificial cofactors into naturally occurring protein scaffolds / Koji Oohora and Takashi Hayashi -- Computational redesign of metalloenzymes for catalyzing new reactions / Per Jr. Greisen and Sagar D. Khare.
  • 2006 Springer Protocols
    edited by Raphael Guerois, Manuela López de la Paz.
    Design of structural elementary motifs. Structure and stability of the [alpha]-helix: lessons for design / Neil Errington, Teuku Iqbalsyah, and Andrew J. Doig. De novo design of monomeric [beta]-hairpin and [beta]-sheet peptides / David Pantoja-Uceda, Clara M. Santiveri, and M. Angeles Jiménez. De novo proteins from binary-patterned combinatorial libraries / Luke H. Bradley, Peter P. Thumfort, and Michael H. Hecht. Non-protein amino acids in the design of secondary structure scaffolds / Radhakrishnan Mahalakshmi and Padmanabhan Balaram. Design and synthesis of [beta]-peptides with biological activity / Marc J. Koyack and Richard P. Cheng -- Design of entire proteins and protein complexes. Design of miniproteins by the transfer of active sites onto small-size scaffolds / Franc̦ois Stricher, Loïc Martin, and Claudio Vita. Consensus design as a tool for engineering repeat proteins / Tommi Kajander, Aitziber L. Cortajarena, and Lynne Regan. Multiple sequence alignment as a guideline for protein engineering strategies / Alan R. Davidson. Sequence search methods and scoring functions for the design of protein structures / Hocine Madaoui, Emmanuelle Becker, and Raphael Guerois. Prediction of protein-protein interaction based on structure / Gregorio Fernandez-Ballester and Luis Serrano. Electrostatic design of protein-protein association rates / Gideon Schreiber, Yossi Shaul, and Kay E. Gottschalk -- Design of amyloidogenic polypeptides and amyloid inhibitors. Peptide model systems for amyloid fiber formation: design strategies and validation methods / Alexandra Esteras-Chopo, María Teresa Pastor, and Manuela López de la Paz. Protein misfolding disorders and rational design of antimisfolding agents / Lisbell D. Estrada, June Yowtak, and Claudio Soto.
  • 2012 Springer
    edited by Jacqueline M. Matthews.
    Also available: Print – 2012
  • 2014 Springer Protocols
    edited by Dennis R. Livesay.
    Monitoring Side-Chain Dynamics of Proteins Using 2H Relaxation -- CPMG Relaxation Dispersion -- Confocal Single-Molecule FRET for Protein Conformational Dynamics -- Protein Structural Dynamics Revealed by Site-directed Spin Labeling and Multifrequency EPR -- Probing Backbone Dynamics With Hydrogen/Deuterium Exchange Mass Spectrometry -- Carbon-Deuterium Bonds as Non-perturbative Infrared Probes of Protein Dynamics, Electrostatics, Heterogeneity, and Folding -- Balancing Bond, Nonbond and G?-like Terms in Coarse Grain Simulations of Conformational Dynamics -- Tutorial on Building Markov State Models with MSMBuilder and Coarse-graining them with BACE -- Analysis of Protein Conformational Transitions Using Elastic Network Model -- Geometric Simulation of Flexible Motion in Proteins -- Principal Component Analysis: A Method for Determining the Essential Dynamics of Proteins -- A Case Study Comparing Quantitative Stability/Flexibility Relationships Across Five Metallo-?-Lactamases Highlighting Differences within NDM-1 -- Towards Comprehensive Analysis of Protein Family Quantitative Stability/Flexibility Relationships using Homology Models -- Using the COREX/BEST Server to Model the Native State Ensemble -- Morphing Methods to Visualize Coarse-grained Protein Dynamics.
  • 2006 Springer Protocols
    edited by Katja M. Arndt, Kristian M. Müller.
  • 2006 Springer Protocols
    edited by Yawen Bai and Ruth Nussinov.
  • 2007 Springer
    edited by Vladimir N. Uversky, Anthony L. Fink.
  • 2010 Springer Protocols
    edited by Peter Bross and Niels Gregersen.
    Protein misfolding and celluar stress : an overview / Niels Gregersen and Peter Bross -- Protein aggregation diseases : toxicity of soluble prefibrillar aggregates and their clinical significance / Massimo Stefani -- Consequences of stress in the secretary pathway : the ER stress response and its role in the metabolic syndrome / Martin Schröder and Louise Sutcliffe -- What role does mitochondrial stress play in neurodegenerative diseases? / Alicia Mae Pickrell and Carlos Torres Moraes -- Autophagy in disease / Dalibor Mijaljica, Mark Prescott, and Rodney J. Devenish -- Mitophagy and mitoptosis in disease processes / Dalibor Mijaljica, Mark Prescott, and Rodney J. Devenish -- Cellular stress and protein misfolding during aging / Rajiv Vaid Basaiawmoit and Suresh I.S. Rattan -- Measuring consequences of protein misfolding and cellular stress using OMICS techniques / Peter Bross ... [et al.] -- Production of cells with targeted integration of gene variants of human ABC transporter for stable and regulated expression using the Flp recombinase system / Kanako Wakabayashi-Nakao ... [et al.] -- Stress response profile in human fribroblasts exposed to heat shock or oxidative stress / Christina Bak Pedersen and Niels Gregersen -- Examining ubiquitinated protein aggregates in tissue sections / Natalia A. Kaniuk and John H. Brumell -- Determination of proteasomal activities / Cristovao F. Lima and Suresh I.S. Rattan -- Measurement of autophagy in cells and tissues / Isei Tanida and Satoshi Waguri -- Transcription factor sequestration by polyglutamine proteins / Tomoyuki Yamanaka and Nobuyuki Nukina -- Biological membranes as protein aggregation matrices and targets of amyloid toxicity / Monica Bucciantini and Cristina Cecchi -- Measurement of mitochondrial ROS production / Anatoly A. Starkov -- In vivo detection of oxidized proteins : a practical approach to tissue-derived mitochondria / Francesca Maltecca and Giorgio Casari -- Measurement of oxidized/reduced glutathione ratio / Joshua B. Owen and D. Allan Butterfield -- Determination of altered mitochondria ultrastructure by electron microscopy / Shoichi Sasaki -- Assessing bad sub-cellular localization under conditions associated with prevention or promotion of mitochondrial permeability transition-dependent toxicity / Liana Cerioni and Orazio Cantoni -- A cellular viability assay to monitor drug toxicity / Jakob Hansen and Peter Bross -- Rescue of misfolded proteins and stabilization by small molecules / Raymond C. Stevens, javier Sancho, and Aurora Martinez.
  • 2006 Springer Protocols
    edited by Greg Moorhead.
    Analysis of protein phosphatases / Shirish Shenolikar -- A brief introduction to the protein phosphatase families / Tomas Mustelin -- Small molecule inhibitors of Ser/thr protein phosphatases / Mark Swingle, Li Ni, and Richard E. Honkanen -- Synthesis and use of the protein phosphatase affinity matrices microcystin- and microcystin-biotin-sepharose / Greg Moorhead, Timothy A.J. Haystead, and Carol MacKintosh -- Utilizing protein phosphatase inhibitors to define PP2A as a regulator of ataxia-telangiectasia mutated (ATM) / Aaron A. Goodarzi ... [et al.] -- An automated, fluorescence-based method for continuous assay of PP2A activity / Adam M. Wegner ... [et al.] -- An in vivo assay to quantify stable protein phosphatase 2A (PP2A) heterotrimeric species / Matthew S. Gentry, Richard L. Hallberg, and David C. Pallas -- Mutagenesis and expression of the scaffolding Aa and Ab subunits of PP2A / Ralf Ruediger, Jin Zhou, and Gernot Walter -- Isolation and characterization of PP2A holoenzymes containing FLAG-tagged B subunits / Deanna G. Adams and Brian E. Wadzinski -- Purification of PP2A holoenzymes by sequential immunoprecipitation with anti-peptide antibodies / Gernot Walter, Jin Zhou, and Ralf Ruediger -- Purification of PP2Ac from bovine heart / Hue T. Tran ... [et al.] -- Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions / Laura Trinkle-Mulcahy ... [et al.] -- Yeast two-hybrid screens to identify drosophila PP1-binding proteins / Daimark Bennett and Luke Alphey -- Identification of cellular protein phosphatase-1 regulators / David W. Roadcap, Matthew H. Brush, and Shirish Shenolikar -- Assay for 3-way interaction of protein phosphatase-1 (Glc7) with regulatory subunits plus phosphatase inhibitor-2 / Masumi Eto and David L. Brautigan -- Phosphorylation of the protein phosphatase type 1 inhibitor protein, CPI-17, by protein kinase C / Michael P. Walsh ... [et al.] -- Purification of smooth muscle myosin phosphatase using a thiophosphorylated myosin light chain-affinity resin / Merideth Borman and Justin MacDonald -- Proteins interacting with saccharomyces cerevisiae type 1 protein phosphatase catalytic subunit identified by single-step affinity purification and mass spectrometry / Edmund P. Walsh ... [et al.] -- Expression of protein histidine phosphatase in Escherichia coli, purification, and determination of enzyme activity / Nicole Bäumer .. [et al.] -- The use of RNA interference to analyze protein phosphatase function in mammalian cells / Iain Fraser ... [et al.] -- Recognition of a PP2C interaction motif in several plant protein kinases / Niranjan Chakraborty, Ohta Masaru, and Jian-Kang Zhu -- The use of yeast genetic tools to define biological roles of novel protein phosphatases / Joaquín Ariño ... [et al.] -- Targeting of PP2C in budding yeast / Irene Ota and James Mapes -- Phosphatase targets in TOR signaling / Estela Jacinto -- Functional characterization of the small CTD phosphatases (SCPs) / Michele Yeo and Patrick S. Lin -- Genome-scale discovery and characterization of class-specific protein sequences / David Kerk -- Yeast substrate-trapping system for isolating substrates of protein tyrosine phosphatases / Masahide Fukada and Masaharu Noda.
  • 2008 Springer Protocols
    edited by Mohammed J. Zaki and Christopher Bystroff.
    A historical perspective of template-based protein structure prediction / Jun-tao Guo, Kyle Ellrott, and Ying Xu -- The assessment of methods for protein structure prediction / Anna Tramontaro ... [et al.] -- Aligning sequences to structures / Liam James McGuffin -- Protein structure prediction using threading / Jinbo Xu, Feng Jiao, and Libo Yu -- Algorithms for multiple protein structure alignment and structure-derived multiple sequence alignment / Maxim Shatsky, Ruth Nussinov, and Haim J. Wolfson -- Indexing protein structures using suffix trees / Feng Gao and Mohammed J. Zaki -- Hidden Markov models for prediction of protein features / Christopher Bystroff and Anders Krogh -- The pros and cons of predicting protein contact maps / Lisa Bartoli ... [et al.] -- Roadmap methods for protein folding / Mark Moll, David Schwarz, and Lydia E. Kavraki -- Scoring functions for de novo protein structure prediction revisited / Shing-Chung Ngan ... [et al.] -- Protein-protein docking : overview and performance analysis / Kevin Wiehe ... [et al.] -- Molecular dynamics simulations of protein folding / Angel E. Garcia.
  • 2014 Springer Protocols
    edited by Daisuke Kihara.
    Protein structure modeling with MODELLER / Benjamin Webb and Andrej Sali -- RaptorX server : a resource for template-based protein structure modeling / Morten Källberg ... [et al.] -- MULTICOM protein tertiary structure prediction system / Jilong Li ... [et al.] -- Modeling of protein side-chain conformations with RASP / Zhichao Miao, Yang Cao, and Taijiao Jiang -- Direct coupling analysis for protein contact prediction / Faruck Morcos ... [et al.] -- ITScorePro : an efficient scoring program for evaluating the energy scores of protein structures for structure prediction / Sheng-You Huang and Xiaoqin Zou -- Assessing the quality of modelled 3D protein structures using the ModFOLD server / Daniel Barry Roche, Maria Teresa Buenavista, and Liam James McGuffin -- 3D-SURFER 2.0 : web platform for real-time search and characterization of protein surfaces / Yi Xiong ... [et al.] -- SPOT-Seq-RNA : predicting protein-RNA complex structure and RNA-binding function by fold recognition and binding affinity prediction / Yuedong Yang ... [et al.] -- POODLE : Tools Predicting Intrinsically Disordered Regions of Amino Acid Sequence / Kana Shimizu -- Prediction of intrinsic disorder in proteins using MFDp2 / Marcin J. Mizianty, Vladimir Uversky, and Lukasz Kurgan -- Modeling protein-protein complexes using the HADDOCK webserver "modeling protein complexes with HADDOCK" / Gydo C.P. van Zundert and Alexandre M.J.J. Bonvin -- Predicting the structure of protein-protein complexes using the swarmdock web server / Mieczyslaw Torchala and Paul A. Bates -- DOCK/PIERR : web server for structure prediction of Protein-protein complexes / Shruthi Viswanath, D.V.S. Ravikant, and Ron Elber -- Pairwise and multimeric protein-protein docking using the LZerD program Suite / Juan Esquivel-Rodriguez ... [et al.] -- Protocols for efficient simulations of long-time protein dynamics using coarse-grained CABS model / Michal Jamroz, Andrzej Kolinski, and Sebastian Kmiecik.
  • 2013 Springer Protocols
    edited by Mark A. Williams, Tina Daviter.
    Protein-ligand interactions : fundamentals / Mark A. Williams -- Protein sample characterization / Tina Daviter and Remi Fronzes -- Measurement of protein-ligand complex formation / Peter N. Lowe, Cara K. Vaughan, and Tina Daviter -- Isothermal titration calorimetry for studying protein-ligand interactions / Luminita Damian -- Rapid mixing kinetic techniques / Stephen R. Martin and Maria J. Schilstra -- Protein-ligand interactions using SPR systems / Asa Frostell, Lena Vinterback, and Hans Sjobom -- Fluorescence techniques in analysis of protein-ligand interactions / Gabor Mocz and Justin A. Ross -- Circular and linear dichroism spectroscopy for the study of protein-ligand interactions / Tina Daviter, Nikola Chmel, and Alison Rodger -- Analyzing protein-ligand interactions by dynamic NMR spectroscopy / Anthony Mittermaier and Erick Meneses -- Studying metal ion-protein interactions : electronic absorption, circular dichroism, and electron paramagnetic resonance / Liliana Quintanar and Lina Rivillas-Acevedo -- Monitoring protein-ligand interactions by time-resolved FTIR difference spectroscopy / Carsten Kotting and Klaus Gerwert -- Biophysical methods in drug discovery from small molecule to pharmaceutical / Geoffrey Holdgate [and others] -- Biophysical screening for the discovery of small-molecule ligands / Alessio Ciulli -- Screening protein-small molecule interactions by NMR / Ben Davis -- Model membrane systems / Heiko Keller, Remigiusz Worch, and Petra Schwille -- Quantitative fluorescence co-localization to study protein-receptor complexes / Shanica N. Pompey, Peter Michaely, and Katherine Luby-Phelps -- Studying protein-ligand interactions using X-ray crystallography / Andrew P. Turnbull and Paul Emsley -- Molecular fields in ligand discovery / Paul J. Gane and A.W. Edith Chan -- Structure-based virtual screening for novel ligands / William R. Pitt [and others].
  • 2012 Springer Protocols
    edited by Francoise Redini.
    Proteoglycans : gene cloning -- Proteoglycan : site mapping and site-directed mutagenesis -- Mapping of the wnt/beta-catenin/TCF response elements in the human versican promoter -- Gene silencing in mouse embryonic stem cells -- A novel strategy for a splice-variant selective gene ablation : the example of the versican v0/v2 knockout -- Detection of neurocan in cerebrospinal fluid -- Glycosaminoglycan chain analysis and characterization (glycosylation/epimerization) -- Characterization of glycosaminoglycans by tandem vibrational microspectroscopy and multivariate data analysis -- Glycosaminoglycans : oligosaccharide analysis by liquid chromatography, capillary electrophoresis, and specific labeling -- Brain chondroitin/dermatan sulfate, from cerebral tissue to fine structure : extraction, preparation, and fully automated chip-electrospray mass spectrometric analysis -- Use of neutrons reveals the dynamics of cell surface glycosaminoglycans -- Following protein-glycosaminoglycan polysaccharide interactions with differential scanning fluorimetry -- In vivo scintigraphic imaging of proteoglycans -- Serglycin : the master of the mast cell -- Analysis of aggrecan catabolism by immunoblotting and immunohistochemistry -- Heparan sulfate proteoglycans as multifunctional cell regulators : cell surface receptors -- Models for studies of proteoglycans in kidney pathophysiology -- Lumican promotes corneal epithelial wound healing -- Shedding of cell membrane-bound proteoglycans -- Modulatory effects of proteoglycans on proteinase activities -- Proteoglycans and osteolysis -- Proteoglycans and cartilage repair.
  • 2007 Springer
    M.R. Wilkins ... [et al.], eds.
    Also available: Print – 2007
  • 2013 Springer Protocols
    edited by Ming Zhou and Timothy Veenstra.
    Affinity depletion of plasma and serum for mass spectrometry-based proteome analysis / Julian A.J. Jaros ... [et al.] -- Tissue sample preparation for biomarker discovery / Yoshiyuki Suehara, Daisuke Kubota, and Tsuyoshi Saito -- Subcellular fractionation for identification of biomarkers : serial detergent extraction by subcellular accessibility and solubility / Sun-II Hwang and David K. Han -- Analysis of secreted proteins / Valeria Severino, Annarita Farina, and Angela Chambery -- Preparation of human cerebrospinal fluid for proteomics biomarker analysis / Timothy J. Waybright -- Proteomic analysis of frozen tissue samples using laser capture microdissection / Sumana Mukherjee ... [et al.] -- Use of formalin-fixed, paraffin-embedded tissue for proteomic biomarker discovery / David B. Krizman and Jon Burrows -- Phosphopeptide enrichment using offline titanium dioxide columns for phosphoproteomics / Li-Rong Yu and Timothy Veenstra -- iTRAQ-labeling for biomarker discovery / Leroi V. DeSouza, Sébastien N. Voisin, and K.W. Michael Siu -- Analysis of glycoproteins for biomarker discovery / Jintang He ... [et al.] -- SILAC in biomarker discovery / Benjamin C. Orsburn -- Trypsin-mediated 18O/16O labeling for biomarker discovery / Xiaoying Ye ... [et al.] -- Two-dimensional SDS-PAGE fractionation of biological samples for biomarker discovery / Thierry Rabilloud and Sarah Triboulet -- Informatics of protein and posttranslational modification detection via shotgun proteomics / Jerry D. Holman, Surendra Dasari, and David L. Tabb -- Quantitation of met tyrosine phosphorylation using MRM-MS / Zhaojing Meng -- Preparation of human serum for prolactin measurement by multiple reaction monitoring mass spectrometry / Timothy J. Waybright ... [et al.] -- Label-free quantitative shotgun proteomics using normalized spectral abundance factors / Karlie A. Neilson ... [et al.] -- Employment of complementary dissociation techniques for body fluid characterization and biomarker discovery / David M. Good and Dorothea Rutishauser -- Phosphopeptide microarrays for comparative proteomic profiling of cellular lysates / Liqian Gao ... [et al.] -- Tissue preparation for MALDI-MS imaging of protein and peptides / Simona Colantonio and Roberta M. Smith -- Plant proteogenomics : from protein extraction to improved gene predictions / Brett Chapman ... [et al.] -- Label-free differential analysis of murine postsynaptic densities / Scott P. Goulding, Michael J. MacCoss, and Christine C. Wu -- Fractionation of peptides by strong cation-exchange liquid chromatography / King C. Chan and Haleem J. Issaq.
  • 2014 Springer Protocols
    edited by Laura Poliseno.
    Pseudogene redux with new biological significance / Leonardo Salmena -- Contribution of pseudogenes to sequence diversity / Mauno Vihinen -- Computational methods for pseudogene annotation based on sequence homology / Paul M. Harrison -- Computational methods of identification of pseudogenes based on functionality : entropy and GC content / Evgeniy S. Balakirev ... [et al.] -- Methods of identification of pseudogenes based on functionality : hybridization of 18S rRNA and mRNA during translation / Chuanhua Xing -- Whole-genome identification of neutrally evolving pseudogenes using the evolutionary measure dN/dS / Lise Olivia Andrieux and David Torrents Arenales -- Methods to study the occurrence and the evolution of pseudogenes through a phylogenetic approach / Jacques Dainat and Pierre Pontarotti -- Methods for detecting transcribed pseudogenes : PCR on regions of high sequence similarity followed by cloning and sequencing / Wenyong Ding and Jianwu Dai -- RNA amplification for pseudogene detection using RNA-Seq / Stephen C. M. Tsoi and Michael K. Dyck -- GENCODE pseudogenes / Adam Frankish and Jennifer Harrow -- Methods to detect transcribed pseudogenes : RNA-Seq discovery allows learning through features / Camilo Valdes and Enrico Capobianco -- Proteomics techniques for the detection of translated pseudogenes / Nadia Ucciferri and Silvia Rocchiccioli -- Pseudogenes as competitive endogenous RNAs : target prediction and validation / Florian A. Karreth ... [et al.] -- Pseudogenes : a novel source of trans-acting antisense RNAs / Per Johnsson, Kevin V. Morris, and Dan Grandér -- Pseudogene-derived endogenous siRNAs and their function / Wen-Ling Chan and Jan-Gowth Chang -- Methods to study translated pseudogenes : in vitro translation, fusion with a tag/reporter gene, and complementation assay / Anne Parle-McDermott
  • 2014 Springer Protocols
    edited by Roberto Biassoni, Laboratorio Medicina Molecolare, Dipartimento Medicina Traslazionale, Instituto Giannina Gaslini, Genova, Italy, Alessandro Raso, Laboratorio U.O.C. Neurochirugia, Instituto Giannina Gaslini, Genova, Italy.
    Twenty years of qPCR: A mature technology? -- Minimum information necessary for quantitative real-time PCR experiments -- Selection of reliable reference genes for RT-qPCR analysis -- Introduction to digital PCR -- mRNA and microRNA purity and integrity: The key to success in expression profiling -- Mediator probe PCR: Detection of real-time PCR by label-free probes and a universal fluorogenic reporter -- Absolute quantification of viral DNA: The quest for perfection -- A multiplex real-time PCR-platform integrated into automated extraction method for the rapid detection and measurement of oncogenic HPV type-specific viral DNA load from cervical samples -- Real-time PCR detection of mycoplasma pneumoniae in the diagnosis of community-acquired pneumonia -- A sensible technique to detect mollicutes impurities in human cells cultured in GMP condition -- Real-time quantification assay to monitor BCR-ABL1 transcripts in chronic myeloid leukemia -- A reliable assay for rapidly defining transplacental metastasis using quantitative PCR -- Circulating cell-free DNA in cancer -- Gene expression analysis by qPCR in clinical kidney transplantation -- Posttranscriptional regulatory networks: From expression profiling to integrative analysis of mRNA and microRNA data -- Clinical applications using digital PCR -- Developing noninvasive diagnosis for single-gene disorders: the role of digital PCR.
  • 2012 Springer Protocols
    edited by Scott A. Rifkin.
    Backcross populations and near isogenic lines / Rik Kooke, Erik Wijnker, and Joost J.B. Keurentjes -- F₂ designs for QTL analysis / Yuan-Ming Zhang -- Design and construction of recombinant inbred lines / Daniel A. Pollard -- Two flavors of bulk segregant analysis in yeast / Maitreya J. Dunham -- Selecting markers and evaluating coverage / Matthew A. Cleveland and Nader Deeb -- Composite interval mapping and multiple interval mapping : procedures and guidelines for using windows QTL cartographer / Luciano Da Costa E. Silva, Shengchu Wang, and Zhao-Bang Zeng -- Design database for quantitative trait loci (QTL) data warehouse, data mining, and meta-analysis / Zhi-Liang Hu, James M. Reecy, and Xiao-Lin Wu -- Meta-analysis of QTL mapping experiments / Xiao-Lin Wu and Zhi-Liang Hu -- Using eQTLs to reconstruct gene regulatory networks / Lin S. Chen -- Estimation and interpretation of genetic effects with epistasis using the NOIA model / Jose M. Alvarez-Castro, Orjan Carlborg, and Lars Ronnegard -- Identifying QTL for multiple complex traits in experimental crosses / Samprit Banerjee and Nengjun Yi -- Functional mapping of developmental processes : theory, applications, and prospects / Kiranmoy Das [and others] -- Statistical models for genetic mapping in polyploids : challenges and opportunities / Jiahan Li [and others] -- eQTL / Lun Li, Xianghua Zhang, and Hongyu Zhao -- Genetic mapping of quantitative trait loci for disease -- related phenotypes / Marcella Devoto and Mario Falchi -- Quantitative trait locus analysis in haplodiploid hymenoptera / Jssurgen Gadau, Christof Pietsch, and Leo W. Beukeboom.
  • 2014 Springer Protocols
    edited by Adriana Fontes, Beate Saegesser Santos.
  • 2006 Springer Protocols
    edited by Charles Z. Hotz, Marcel Bruchez.
    Immunofluorescent labeling of proteins in cultured cells with quantum dot secondary antibody conjugates / Richard L. Ornberg and Hongjian Liu -- Immunohistochemical detection with quantum dots / Rizwan S. Akhtar ... [et al.] -- Quantum dots for multicolor clinical pathology and multispectral imaging / Johbu Itoh and Robert Yoshiyuki Osamura -- Light and electron microscopic localization of multiple proteins using quantum dots / Thomas J. Deerinck ... [et al.] -- Human metaphase chromosome fluorescence in situ hybridization using quantum dot conjugates / Joan H.M. Knoll -- Biotin-ligand complexes with streptavidin quantum dots for in vivo cell-labeling of membrane receptors / Diane S. Lidke ... [et al.] -- Single quantum dot tracking of membrane receptors / Cødric Bouzigues ... [et al.] -- Optical monitoring of single cells using quantum dots / Jyoti K. Jaiswal and Sanford M. Simon -- Peptide-mediated intracellular delivery of quantum dots / B. Christoffer Lagerholm -- High-content analysis in multiple cell lines using quantum dots / Paul G. Wylie -- Measuring cell motility using quantum dot probes / Weiwei Gu ... [et al.] -- Quantum dots for in vivo molecular and cellular imaging / Xiaohu Gao, Leland W.K. Chung, and Shuming Nie -- Sentinel lymph node mapping with type II quantum dots / John V. Frangioni ... [et al.] -- Macrophage-mediated colocalization of quantum dots in experimental glioma / Osman Muhammad, Alexandra Popescu, and Steven A. Toms -- Applications of quantum dots to multicolor flow cytometry / Pratip K. Chattopadhyay and Mario Roederer -- Quantum dots in flow cytometry / Barnaby Abrams and Tim Dubrovsky -- Luminescent biocompatible quantum dots: a tool for immunosorbent assay design / Ellen R. Goldman ... [et al.] -- Fluorescence-based analysis of cellular protein lysate arrays using quantum dots / David H. Geho ... [et al.] -- Application of quantum dots to multicolor microarray experiments: four-color genotyping / George Karlin-Neumann ... [et al.].
  • 2011 Springer Protocols
    edited by Kendra P. Rumbaugh.
    Bioassays of quorum sensing compounds using Agrobacterium tumefaciens and Chromobacterium violaceum -- Detection of 2-alkyl-4-quinolones using biosensors -- FRET-based biosensors for the detection and quantification of AI-2 class of quorum sensing compounds -- Isolation of agr quorum sensing autoinducers -- Liquid chromatography/mass spectrometry for the detection and quantification of N-acyl-l-homoserine lactones and 4-hydroxy-2-alkylquinolines -- Detection of autoinducer (AI-2)-like activity in food samples -- Detection of bacterial signaling molecules in liquid or gaseous environments -- Determination of acyl homoserine lactone and tetramic acid concentrations in biological samples -- Luminescent reporters and their applications for the characterization of signals and signal-mimics that alter LasR-mediated quorum sensing -- Modulation of mammalian cell processes by bacterial quorum sensing molecules -- Imaging N-acyl homoserine lactone quorum sensing in vivo -- Defining the structure and function of acyl-homoserine lactone autoinducers -- Global expression analysis of quorum-sensing controlled genes -- Small RNA target genes and regulatory connections in the Vibrio cholerae quorum sensing system -- Quantifying pseudomonas aeruginosa quinolones and examining their interactions with lipids -- Linking quorum sensing regulation and biofilm formation by Candida albicans -- Design of synthetic mammalian quorum-sensing systems -- Qualitative and quantitative determination of quorum sensing inhibition in vitro -- Custom synthesis of autoinducers and their analogues -- Heterologous overexpression, purification, and in vitro characterization of ahl lactonases -- High-performance liquid chromatography analysis of N-acyl homoserine lactone hydrolysis by paraoxonases -- Generation of quorum quenching antibodies.
  • 2012 Springer
    edited by William S. Spielman, Narayanan Parameswaran.
    Introduction to RAMPs / Narayanan Parameswaran and William S. Spielman -- RAMPs and CGRP receptors / James Barwell ... [et al.] -- Regulation of GPCR trafficking by RAMPs / Jennifer M. Bomberger, Narayanan Parameswaran, and William S. Spielman -- Regulation of calcium sensing receptor trafficking by RAMPs / Tristan Bouschet, Støphane Martin, and Jeremy M. Henley -- Understanding RAMPs through genetically engineered mouse models / Mahita Kadmiel, Kimberly L. Fritz, and Kathleen M. Caron -- RAMPs as drug targets / Patrick M. Sexton ... [et al.] -- RAMP like proteins : RTP and reep family of proteins / Joel Mainland and Hiroaki Matsunami -- Regulation of RAMP expression in diseases / Asha Jacob, Rongqian Wu, and Ping Wang -- Perspectives on some recent studies on RAMPs / Narayanan Parameswaran and William S. Spielman.
    Also available: Print – 2012
  • 2012 Springer Protocols
    edited by Anthony P. Davenport.
    Receptor databases and computational websites for ligand binding / Brinda K. Rana, Philip E. Bourne, and Paul A. Insel -- How to use the IUPHAR receptor database to navigate pharmacological data / Chidochangu P. Mpamhanga [and others] -- Radioligand binding assays and their analysis / Janet J. Maguire, Rhoda E. Kuc, and Anthony P. Davenport -- Use of scintillation proximity assay to measure radioligand binding to immobilized receptors without separation of bound from free ligand / Jenny Berry, Molly Price-Jones, and Barbara Killian -- Visualization and analysis of vascular receptors using confocal laser scanning microscopy and fluorescent ligands / Craig J. Daly, Ingela Parmryd, and John C. McGrath -- Dissecting the pharmacology of G protein-coupled receptor signaling complexes using bimolecular fluorescence complementation / Laura E. Kilpatrick and Nicholas D. Holliday -- Live cell imaging of G protein-coupled receptors / Anke Teichmann [and others] -- Characterization of G-protein coupled receptor modulators using homogeneous cAMP assays / Daniel L. Bassoni [and others] -- Measurements of [beta]-arrestin recruitment to activated seven transmembrane receptors using enzyme complementation / Daniel L. Bassoni [and others] -- Quantitative phosphor imaging autoradiography of radioligands for positron emission tomography / Peter Johnstrom, Joseph L. Bird, and Anthony P. Davenport -- Dynamic in vivo imaging of receptors in small animals using positron emission tomography / Peter Johnstrom [and others] -- Cellular localization of receptors using antibodies visualized by light and dual labeling confocal microscopy / Anthony P. Davenport and Rhoda E. Kuc -- Detection of mRNA encoding receptors by in situ and northern hybridization / Alessandra P. Princivalle [and others].
  • 2015 Springer Protocols
    edited by Serena Germano.
    Analysis of receptor tyrosine kinase (RTK) phosphorylation by immunoblotting / Martina McDermott and Norma O'Donovan -- Analysis of changes in phosphorylation of receptor tyrosine kinases : antibody arrays / Sweta Rani and Lorraine O'Driscoll -- Analysis of epidermal growth factor receptor dimerization by BS³ cross-linking / Harmony F. Turk and Robert S. Chapkin -- Single-molecule optical methods analyzing receptor tyrosine kinase activation in living cells / Inhee Chung and Ira Mellman -- Evaluation of the dimerization profiles of HER tyrosine kinases by time-resolved Förster resonance energy transfer (TR-FRET) / Evelyne Lopez-Crapez [and three others] -- Applying the proximity ligation assay (PLA) to mouse preimplantation embryos for identifying protein-protein interactions in situ / Ivan Bedzhov and Marc P. Stemmler -- Analysis of receptor tyrosine kinase gene amplification on the example of FGFR1 / Diana Boehm, Anne von Mässenhausen, and Sven Perner -- Quantification of the effects of mutations on receptor tyrosine kinase (RTK) activation in mammalian cells / Lijuan He and Kalina Hristova -- Cell surface biotinylation of receptor tyrosine kinases to investigate intracellular trafficking / Mathieu J.F. Crupi, Douglas S. Richardson, and Lois M. Mulligan -- Studying N-linked glycosylation of receptor tyrosine kinases / Harri M. Itkonen and Ian G. Mills -- Identification of receptor protein tyrosine phosphatases (RPTPs) as regulators of receptor tyrosine kinases (RTKs) using an RPTP siRNA-RTK substrate screen / Hojin Lee and Anton M. Bennett -- Downregulation of receptor tyrosine kinases through ubiquitination : analysis by immunodetection / Noriaki Shimokawa and Noriyuki Koibuchi -- Regulation of receptor tyrosine kinases by miRNA : overexpression of miRNA using lentiviral inducible expression vectors / XiangDong Le, Andrew T. Huang, Yunyun Chen, and Stephen Y. Lai -- Human tumor xenografts in mouse as a model for evaluating therapeutic efficacy of monoclonal antibodies or antibody-drug conjugate targeting receptor tyrosine kinases / Liang Feng [and five others] -- Receptor tyrosine kinase targeting in multicellular spheroids / Susan Breslin and Lorraine O'Driscoll -- Receptor tyrosine kinases and drug resistance : development and characterization of in vitro models of resistance to RTK inhibitors / Claire Corcoran and Lorraine O'Driscoll.
  • 2012 Springer Protocols
    edited by Argelia Lorence.
    Using folding promoting agents in recombinant protein production : a review / Beatrix Fahnert -- Routine identity confirmation of recombinant proteins by MALDI-TOF mass spectrometry / Brett J. Savary and Prasanna Vasu -- A matter of packaging : influence of nucleosome positioning on heterologous gene expression / María de la Cruz Muñoz-Centeno, Gonzalo Millán-Zambrano, and Sebastián Chávez -- Tools of the trade : developing antibody-based detection capabilities for recombinant proteins / Maureen C. Dolan [and others] -- Heat-shock protein fusion vectors for improved expression of soluble recombinant proteins in Escherichia coli / Christos A. Kyratsous and Christos A. Panagiotidis -- The use of a flagellar export signal for the secretion of recombinant proteins in salmonella / Ferenc Vonderviszt [and others] -- Optimization of purification protocols based on the step-by-step monitoring of the protein aggregates in soluble fractions / Ario de Marco -- Heterologous protein expression by Lactococcus lactis / Julio Villator-Hernández [and others] -- An extended suite of genetic tools for use in bacteria of the halomonadaceae : an overview / Montserrat Argandoña [and others] -- Regulated recombinant protein production in the antarctic bacterium Pseudoalteromonas haloplanktis TAC125 / Valentina Rippa [and others] -- A novel strategy for the construction of genomic mutants of the antarctic bacterium Pseudoalteromonas haloplanktis TAC125 / Maria Giuliani [and others] -- A new bacterial co-expression system for over-expressing soluble protein and validating protein-protein interaction / Jumei Zeng and Zheng-Guo He -- Heterologous high-level gene expression in the photosynthetic bacterium Rhodobacter capsulatus / Nadine Katzke [and others] -- Plasmid DNA production for therapeutic applications / Alvaro R. Lara and Octavio T. Ramírez -- Recombinant protein production in the eukaryotic protozoan parasite Leishmania tarentolae : a review / Tomoaki Niimi -- Expression of multisubunit proteins in Leishmania tarentolae / Marisa Sugino and Tomoaki Niimi -- Recombinant protein production in yeasts / Diethard Mattanovich [and others] -- Yeasts as a tool for heterologous gene expression / Raja Mokdad-Gargouri [and others] -- The Cre/Lox system : a practical tool to efficiently eliminate selectable markers in fungal endophytes / Simona Florea [and others] -- Aptamer-regulated expression of essential genes in yeast / Beatrix Suess [and others] -- Cloning and expression of hemicellulases from Aspergillus nidulans in Pichia pastoris / Prasanna Vasu, Stefan Bauer, and Brett J. Savary -- A thiamine-regulatable epitope-tagged protein expression system in fission yeast / Tiina Tamm -- Heterologous gene expression by chromosomal integration in fission yeast / Akihisa Matsuyama and Minoru Yoshida -- Genetic engineering of industrial strains of Saccharomyces cerevisiae / Sylvie le Borgne -- Recombinant protein production in plants : challenges and solutions / Elizabeth E. Hood and Deborah V. Vicuna Requesens -- A novel plant cell bioproduction platform for high-yield secretion of recombinant proteins / Jianfeng Xu and Marcia J. Kieliszewski -- Super-promoter :TEV, a powerful gene expression system for tobacco hairy roots / Luis Ñopo [and others] -- Bioseparation of recombinant proteins from plant extract with hydrophobin fusion technology / Jussi J. Joensuu [and others] -- Quality assessment of recombinant proteins produced in plants / Giuliana Medrano [and others] -- Cell-free protein synthesis as a promising expression system for recombinant proteins / Xumeng Ge and Jianfeng Xu -- The use of bacterial artificial chromosomes for recombinant protein production in mammalian cell lines / Leander Blaas [and others] -- Engineering the chaperone network of CHO cells for optimal recombinant protein production and authenticity / Lyne Jossé, C. Mark Smales, and Mick F. Tuite -- High-throughput baculovirus expression in insect cells / Richard B. Hitchman, Robert D. Possee, and Linda A. King -- Recombinant protein expression in milk of livestock species / Zsuzsanna Bösze and László Hiripi.
  • 2012 Springer Protocols
    edited by Roslyn M. Bill.
    Optimising yeast as a host for recombinant protein production (review) / Nicklas Bonander and Roslyn M. Bill -- Which yeast species shall I choose? : Saccharomyces cerevisiae versus Pichia pastoris (review) / Richard A.J. Darby [and others] -- Preparation of Pichia pastoris expression plasmids / Christel Logez [and others] -- Preparation of Saccharomyces cerevisiae expression plasmids / David Drew and Hyun Kim -- Codon optimisation for heterologous gene expression in yeast / Kristina Hedfalk -- Yeast transformation to generate high-yielding clones / Mohammed Jamshad and Richard A.J. Darby -- Screening for high-yielding Pichia pastoris clones : the production of G protein-coupled receptors as a case study / Shweta Singh [and others] -- Screening for high-yielding Saccharomyces cerevisiae clones: using a green fluorescent protein fusion strategy in the production of membrane proteins / David Drew and Hyun Kim -- The effect of antifoam addition on protein production yields / Sarah J. Routledge and Roslyn M. Bill -- Setting up a bioreactor for recombinant protein production in yeast / Sarah J. Routlledge and Michelle Clare -- The implementation of a design of experiments strategy to increase recombinant protein yields in yeast (review) / Nagamani Bora [and others] -- Online analysis and process control in recombinant protein production (review) / Shane M. Palmer and Edmund R.S. Kunji -- Monitoring the biomass accumulation of recombinant yeast cultures : offline estimations of dry cell mass and cell counts / Shane M. Palmer and Edmund R.S. Kunji -- Online monitoring of biomass accumulation in recombinant yeast cultures / Shane M. Palmer and Edmund R.S. Kunji -- Optimising Pichia pastoris induction / Zharain Bawa and Richard A.J. Darby -- Optimizing Saccharomyces cerevisiae induction regimes / David Drew and Hyun Kim -- Large-scale production of membrane proteins in Pichia pastoris : the production of G protein-coupled receptors as a case study / Shweta Singh [and others] -- Large-scale production of secreted proteins in Pichia pastoris / Nagamani Bora -- Disruption of yeast cells to isolate recombinant proteins / Mohammed Jamshad and Richard A.J. Darby.
  • 2008 Springer
    volume editors, Richard Egel, Dirk-Henner Lankenau.
    "Once per life cycle, mitotic nuclear divisions are replaced by meiosis I and II - reducing chromosome number from the diploid level to a haploid genome, reshuffling the homologous chromosomes by their centromeres, and recombining chromosome arms by crossing-over. In animals, including humans, all this happens during the germ cell formation of eggs and sperm. Due to the reign of meiosis, no child is a true genetic copy of either parent. Questions still open primarily concern the placement of and mutual coordination between neighboring crossover events. The current book addresses these processes and mechanisms in multicellular eukaryotes, such as Drosophila, Anibidopsis, mice and humans. The pioneering model systems of yeasts, as well as evolutionary aspects, will be addressed in a forthcoming volume."--Jacket.
  • 2008 Springer
    volume editors, Richard Egel, Dirk-Henner Lankenau.
    Once per life cycle, mitotic nuclear divisions are replaced by meiosis I and II - reducing chromosome number from the diploid level to a haploid genome and recombining chromosome arms by crossing-over. In animals, all this happens during formation of eggs and sperm - in yeasts before spore formation. The mechanisms of reciprocal exchange at crossover/chiasma sites are central to mainstream meiosis. To initiate the meiotic exchange of DNA, surgical cuts are made as a form of calculated damage that subsequently is repaired by homologous recombination. These key events are accompanied by ancillar.
  • 2011
    Andrea Elise Hartsock.
    Throughout development cells must go through numerous changes in cell-cell adhesiveness. Within an epithelial layer, cell-cell contacts form and maintenance junctional complexes such as Adherens Junctions, Tight Junctions, and Desmosomes. The Adherens junction (AJ) and Tight junction (TJ) provide important adhesive contacts between neighboring epithelial cells. Although these junctions comprise different proteins, there are similarities in the roles of specialized transmembrane proteins in forming extracellular adhesive contacts between cells, and intracellular links to the actin cytoskeleton and signaling pathways including the regulation of gene transcription. The Adherens junction performs multiple functions including initiation and stabilization of cell--cell adhesion, regulation of the actin cytoskeleton, intracellular signaling and transcriptional regulation. The core of the Adherens junction includes interactions among transmembrane glycoproteins of the classical cadherin superfamily, such as E-cadherin, and the catenin family members including p120-catenin, [Beta]-catenin, and [Alpha]-catenin. Together, these proteins control the formation, maintenance and function of adherens junctions. Tight junctions have been proposed to have two mutually exclusive functions: a fence function which prevents the mixing of membrane proteins between the apical and basolateral membranes; and a gate function which controls the paracellular passage of ions and solutes in-between cells. Tight junctions contain two types of transmembrane proteins, occludins and claudins, which confer these functions, and associated cytoplasmic proteins that may link tight junctions to the actin-cytoskeleton and the adherens junction. Adherens Junction mediated cell--cell adhesion is highly dynamic enabling the reorganization and dispersal of cells, for example, during epithelial-to-mesenchymal transition in normal development and carcinogenesis. p120-Catenin binding to, and Hakai (an E3 ubiquitin ligase)-mediated ubiquitination of the juxtamembrane domain (JMD) of E-cadherin are thought to be involved in regulating E-cadherin internalization and degradation. However, the relationship between these two pathways is not known. The aim of my project was to determine if p120-catenin/E-cadherin and Hakai dependent ubiquitination of E-cadherin are mechanistically linked. We targeted the E-cadherin JMD to mitochondria (WT-JMD) to isolate this domain from the plasma membrane and internalization, and to examine protein modifications and degradation. WT-JMD was targeted to mitochondria, but it did not accumulate there except when proteasome activity was inhibited. We found that WT-JMD was ubiquitinated, and arginine substitution of lysines at position 5 (K5R) and 83 (K83R) resulted in the stable accumulation of mutant JMD at mitochondria. p120-Catenin did not localize, or bind to WT-JMD even upon proteasome inhibition, whereas the K5,83R JMD mutant bound p120-catenin and localized it to mitochondria. Mutation of the p120-catenin binding site in combination with these lysine mutations inhibited p120-catenin bind-ing, but did not decrease JMD stability or its accumulation at mitochondria. Further-more, over-expression of Hakai resulted in inhibition of p120-catenin binding to WT-JMD. Thus, increased stability of JMD lysine mutants was due to inhibition of ubiquitination and not to p120-catenin binding. Finally, mutation of these critical lysines in full length E-cadherin had the same effects on protein stability as mitochondria-targeted E-cadherin JMD. Our results indicate that ubiquitination of the JMD inhibits p120-catenin binding, and targets E-cadherin for degradation via the proteasome. Further work needs to be done to test our hypothesis that p120-catenin stabilizes E-cadherin by masking ubiquitination sites within E-cadherin-JMD.
  • 2016 Springer
    Sajal Chakraborti, Naranjan S. Dhalla, editors.
  • 2015 Springer Protocols
    2014 Springer Protocols
    edited by Gordon G. Carmichael.
    Diverse functions and mechanisms of mammalian long noncoding RNAs -- Sequence-defined oligoaminoamides for the delivery of siRNAs -- A biochemical approach to identify direct microRNA targets -- Native gel analysis for mammalian microRNPs assembled from pre-microRNAs -- Identification and validation of miRNA target sites within nontraditional miRNA targets -- Fractionation of non-polyadenylated and ribosomal-free RNAs from mammalian cells -- Affinity purification of long noncoding RNA-protein complexes from formaldehyde cross-linked mammalian cells -- RNA pulldown protocol for in vitro detection and identification of RNA-associated proteins -- nRIP-seq: A technique to identify RNA targets of an RNA binding protein on a genome-wide scale -- Analysis of the subcellular distribution of RNA by fluorescence in situ hybridization -- RNA fluorescence in situ hybridization in cultured mammalian cells -- Locating RNAs in situ with FISH-STIC probes -- Rapid and efficient elimination of specific nuclear noncoding RNAs in mammalian cells with antisense oligonucleotides -- On using magnesium and potassium ions in RNA experiments -- Studying the affinity, kinetic stability, and specificity of RNA/protein interactions: SINE ncRNA/Pol II complexes as a model system
  • 2012 Springer
    Wolfgang R. Hess, Anita Marchfelder.
    This book provides a comprehensive and up-to-date collection of review articles focusing on RNA-mediated regulation in prokaryotes. The various modes of action include the direct interaction with proteins, direct sensing of metabolites or of physical parameters, and the interaction with RNAs to stimulate or prevent binding of ribosomes or to stimulate degradation. Written by leading experts in the field, the book covers small RNA functions, RNA thermometers, riboswitches, the diversity of small RNA-guided CRISPR-Cas defense systems and selected RNA chaperons in both prokaryotic domains, bacteria and archaea. Recent advances towards the computational identification of regulatory RNAs and their targets are included and particular attention is paid to small RNA in pathogenic bacteria. This volume is the only one exclusively covering regulatory RNAs in the prokaryotic domains to date, making it essential literature for anyone interested in RNA function and gene regulation and a valuable resource for teaching these concepts.
  • 2012 Karger
    volume editor, Manuel A. Garrido-Ramos.
    The Repetitive DNA Content of Eukaryotic Genomes / López-Flores, I.; Garrido-Ramos, M.A. -- Telomere Dynamics in Mammals / Silvestre, D.C.; Londoño-Vallejo, A. -- Drosophila Telomeres: an Example of Co-Evolution with Transposable Elements / Silva-Sousa, R.; López-Panadès, E.; Casacuberta, E. -- The Evolutionary Dynamics of Transposable Elements in Eukaryote Genomes / Tollis, M.; Boissinot, S. -- SINEs as Driving Forces in Genome Evolution / Schmitz, J. -- Unstable Microsatellite Repeats Facilitate Rapid Evolution of Coding and Regulatory Sequences / Jansen, A.; Gemayel, R.; Verstrepen, K.J. -- Satellite DNA Evolution / Plohl, M.; Meštrović, N.; Mravinac, B. -- Satellite DNA-Mediated Effects on Genome Regulation / Pezer, Ž.; Brajković, J.; Feliciello, I.; Ugarković, Đ. -- The Birth-and-Death Evolution of Multigene Families Revisited / Eirín-López, J.M.; Rebordinos, L.; Rooney, A.P.; Rozas, J. -- Chromosomal Distribution and Evolution of Repetitive DNAs in Fish / Cioffi, M.B.; Bertollo, L.A.C..
  • 2009 Springer
    Pedro J. Chedrese, editor.
    Basis for molecular reproductive biology -- Introduction to the molecular organization of the endocrine/reproductive system -- Extracellular signaling receptors -- Molecules that transmit information into the cell: the intracellular signaling pathways -- Introduction to molecular biology: structure and function of the macromolecules of genetic information -- Regulation of gene expression -- Molecular basis of abnormal phenotype -- Tools of molecular reproductive biology -- Recombinant DNA technology -- Techniques for DNA analysis -- Analyzing gene expression -- DNA microarray analysis -- Computer assisted analysis of genes -- Introduction to gene therapy -- Molecular regulation of reproductive hormones -- GnRH-GnRH-receptor system in the mammalian female reproductive tract -- FSH: one hormone with multiple forms, or a family of multiple hormones -- FSH receptor: one receptor with multiple forms or a family of receptors -- Regulation of the early steps in gonadal steroidogenesis -- Prostaglandins and their mechanisms of action in the cyclic ovary -- Androgens: molecular basis and related disorders -- Leptin as a reproductive hormone -- Neurosteroids and sexual behavior and reproduction -- Molecular regulation of the reproductive organs and tissues -- Autocrine and paracrine regulation of the ovary -- Ovarian endocrine activity: role of follistatin, activin, and inhibin -- Hormonal and molecular regualtion of the cytochrome P450 aromatase gene expression in the ovary -- Epigenetic mechanisms of ovarian gene regulation -- Ovarian function and failure: the role of the oocyte and its molecules -- Molecular control of corpus luteum function -- Molecular landscape of spermatogonial stem cell renewal, meiotic sex chromosome inactivation, and spermatic head shaping.
  • Springer, 2005
    Satoru Miyano, Jill Mesirov, Simon Kasif, Sorin Istrail, Pavel Pevzner, Michael Waterman (eds.).
  • Springer, 2007
    Terry Speed, Haiyan Huang (eds.).
  • 2008 Springer
    Martin Vingron, Limsoon Wong (eds.).
  • Springer, 2006
    Alberto Apostolico, Concettina Guerra, Sorin Istrail, Pavel Pevzner, Michael Waterman (eds.).
  • 2013 Springer
    Mieczyslaw Pokorski, editor.
    Also available: Print – 2013
  • 2010 Springer
    Fenyong Liu, Sidney Altman, editors.
    History of RNase P and Overview of Its Catalytic Activity -- The Evolution of RNase P and Its RNA -- Over a Decade of Bacterial Ribonuclease P Modeling -- Structural Studies of Ribonuclease P -- Folding of Bacterial RNase P RNA -- Kinetic Mechanism of Bacterial RNase P -- Roles of Metal Ions in RNase P Catalysis -- Challenges in RNase P Substrate Recognition: Considering the Biological Context -- Archaeal RNase P: A Mosaic of Its Bacterial and Eukaryal Relatives -- Eukaryote RNase P and RNase MRP -- RNase P from Organelles -- Human RNase P and Transcription -- RNase P as a Drug Target -- Ribonuclease P as a Tool.
  • 2012 Springer Protocols
    edited by Jörg S. Hartig.
    Introduction -- Characterization of hammerhead ribozyme reactions -- Mechanistic analysis of the hepatitis delta virus (HDV) ribozyme: Methods for RNA preparation, structure mapping, solvent isotope effects, and co-transcriptional cleavage -- Kinetic characterization of hairpin ribozyme variants -- Characterization of RNase p rna activity -- Group I intron ribozymes -- Kinetic characterization of group II intron folding and splicing -- Mechanism and distribution of glmS ribozymes -- Structure-based search and in vitro analysis of self-cleaving ribozymes -- Discovery of rna motifs using a computational pipeline that allows insertions in paired regions and filtering of candidate sequences -- Crystallographic analysis of small ribozymes and riboswitches -- Functional dynamics of RNA ribozymes studied by NMR spectroscopy -- Deoxyribozyme-based, semisynthetic access to stable peptidyl-trnas exemplified by tRNA(Val) carrying a macrolide antibiotic resistance peptide -- Probing functions of the ribosomal peptidyl transferase center by nucleotide analog interference -- Single molecule fret characterization of large ribozyme folding -- Metal ion-RNA interactions studied via multinuclear NMR -- Analysis of catalytic RNA structure and function by nucleotide analog interference mapping -- In vitro selection of metal ion-selective dnazymes -- Selecting allosteric ribozymes -- Screening effective target sites on mRNA: A ribozyme library approach -- A computational approach to predict suitable target sites for trans-acting minimal hammerhead ribozymes -- Targeting mRNAs by engineered sequence-specific RNase p ribozymes -- Target-induced SOFA-HDV ribozyme -- Ribozyme-mediated trans insertion-splicing into target RNAs -- Developing fluorogenic RNA-cleaving dnazymes for biosensing applications -- Development of trainable deoxyribozyme-based game playing automaton -- Rational design and tuning of ribozyme-based devices -- In vivo screening of ligand-dependent hammerhead ribozymes -- Flexizymes as a tRNA acylation tool facilitating genetic code reprogramming.
  • 2001 ScienceDirect
    editors, Dieter Söll, Susumu Nishiura, Peter Moore.
  • 2008 Springer Protocols
    edited by Henrik Nielsen.
    The transcriptional landscape -- Working with RNA -- Synthesis of RNA by in vitro transcription -- Efficient poly(A)+ RNA selection using LNA oligo(T) capture -- Genome browsers -- Web-based tools for studying RNA structure and function -- Northern blotting analysis -- Rapid amplification of cDNA ends (RACE) -- Fractionation of mRNA based on the length of the poly(A) tail -- Analysis of mutations that influence pre-mRNA splicing -- S1 nuclease analysis of alternatively spliced mRNA -- Promises and challenges in developing RNAi as a research tool and therapy -- Inhibition of gene function in mammalian cells using short-hairpin RNA (shRNA) -- Validation of RNAi by real time PCR -- Profiling RNA polymerase II using the fast chromatin immunoprecipitation method -- The post-transcriptional operon -- RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray (chip) profiling -- Isolation of RNP granules -- Electrophoretic mobility shift assay for characterizing RNA-protein interaction -- Polysome analysis and RNA purification from sucrose gradients -- Prediction of targets for microRNAs -- Outsourcing of experimental work.
  • 2012 Springer Protocols
    edited by Hailing Jin, Walter Gassmann.
    SuperSAGE : powerful serial analysis of gene expression / Hideo Matsumura [and others] -- Northern blot analysis for expression profiling of mRNAs and small RNAs / Ankur R. Bhardwaj [and others] -- Construction of RNA-seq libraries from large and microscopic tissues for the illumina sequencing platform / Hagop S. Atamian and Isgouhi Kaloshian -- Strand-specific RNA-seq applied to malaria samples / Nadia Ponts, Duk-Won D. Chung, and Karine G. Le Roch -- RNA in situ hybridization in Arabidopsis / Miin-Feng Wu and Doris Wagner -- Laser microdissection of cells and isolation of high-quality RNA after cryosectioning / Marta Barcala, Carmen Fenoll, and Carolina Escobar -- Detection and quantification of alternative splicing variants using RNA-seq / Douglas W. Bryant Jr, Henry D. Priest, and Todd C. Mockler -- Separating and analyzing sulfur-containing RNAs with Organomercury Gels / Elisa Biondi and Donald H. Burke -- RNAse mapping and quantitation of RNA isoforms / Lakshminarayan K. Venkatesh, Olufemi Fasina, and David J. Pintel -- Detection and quantification of viral and satellite RNAs in plant hosts / Sun-Jung Kwon, Jang-Kyun Seo, and A.L.N. Rao -- In situ detection of mature miRNAs in plants using LNA-modified DNA probes / Xiaozhen Yao, Hai Huang, and Lin Xu -- Small RNA isolation and library construction for expression profiling of small RNAs from neurospora and fusarium using illumina high-throughput deep sequencing / Gyungsoon Park and Katherine A. Borkovich -- Isolation and profiling of protein-associated small RNAs / Hongwei Zhao [and others] -- New virus discovery by deep sequencing of small RNAs / Kashmir Singh, Ravneet Kaur, and Wenping Qiu -- Global assembly of expressed sequence tags / Foo Cheung -- Computational analysis of RNA-seq / Scott A. Givan, Christopher A. Bottoms, and William G. Spollen -- Identification of microRNAs and natural antisense transcript-originated endogenous siRNAs from small-RNA deep sequencing data / Weixiong Zhang [and others].
  • 2012 Springer
    Neocles Leontis, Eric Westhof, editors.
    Introduction / Michael Levitt -- Modeling RNA Molecules / Neocles Leontis and Eric Westhof -- Methods for Predicting RNA Secondary Structure / Kornelia Aigner, Fabian Dre€en and Gerhard Steger -- Why Can't We Predict RNA Structure At Atomic Resolution? / Parin Sripakdeevong, Kyle Beauchamp and Rhiju Das -- Template-Based and Template-Free Modeling of RNA 3D Structure: Inspirations from Protein Structure Modeling / Kristian Rother, Magdalena Rother, Michał Boniecki, Tomasz Puton and Konrad Tomala, et al. -- The RNA Folding Problems: Different Levels of sRNA Structure Prediction / Fredrick Sijenyi, Pirro Saro, Zheng Ouyang, Kelly Damm-Ganamet and Marcus Wood, et al. -- Computational Prediction and Modeling Aid in the Discovery of a Conformational Switch Controlling Replication and Translation in a Plus-Strand RNA Virus / Wojciech K. Kasprzak and Bruce A. Shapiro -- Methods for Building and Refining 3D Models of RNA / Samuel C. Flores, Magdalena Jonikas, Christopher Bruns, Joy P. Ku and Jeanette Schmidt, et al. -- Multiscale Modeling of RNA Structure and Dynamics / Feng Ding and Nikolay V. Dokholyan -- Statistical Mechanical Modeling of RNA Folding: From Free Energy Landscape to Tertiary Structural Prediction / Song Cao and Shi-Jie Chen -- Simulating Dynamics in RNA-Protein Complexes / John Eargle and Zaida Luthey-Schulten -- Quantum Chemical Studies of Recurrent Interactions in RNA 3D Motifs / Jiří Šponer, Judit E. Šponer and Neocles B. Leontis -- Nonredundant 3D Structure Datasets for RNA Knowledge Extraction and Benchmarking / Neocles B. Leontis and Craig L. Zirbel -- Ions in Molecular Dynamics Simulations of RNA Systems / Pascal Auffinger -- Modeling RNA Folding Pathways and Intermediates Using Time-Resolved Hydroxyl Radical Footprinting Data / Joshua S. Martin, Paul Mitiguy and Alain Laederach -- A Top-Down Approach to Determining Global RNA Structures in Solution Using NMR and Small-Angle X-ray Scattering Measurements / Yun-Xing Wang, Jinbu Wang and Xiaobing Zuo -- RNA Structure Determination by Structural Probing and Mass Spectrometry: MS3D / A. E. Hawkins and D. Fabris.
  • 2011 Springer Protocols
    edited by Jeffrey E. Gerst.
    Single molecule imaging of RNA in situ / Mona Batish, Arjun Raj, and Sanjay Tyagi -- FISH and immunofluorescence staining in Chlamydomonas / James Uniacke, Daniel Colón-Ramos, and William Zerges -- High resolution fluorescent in situ hybridization in Drosophila / Eric Lécuyer -- Localization and anchorage of maternal mRNAs to cortical structures of ascidian eggs and embryos using high resolution in situ hybridization / Alexandre Paix, Janet Chenevert, and Christian Sardet -- Visualization of mRNA localization in Xenopus oocytes / James A. Gagnon and Kimberly L. Mowry -- Visualization of mRNA expression in the zebrafish embryo / Yossy Machluf and Gil Levkowitz -- High-resolution fluorescence in situ hybridization to detect mRNAs in neuronal compartments in vitro and in vivo / Sharon A. Swanger, Gary J. Bassell, and Christina Gross -- Localization of mRNA in vertebrate axonal compartments by in situ hybridization / José Roberto Sotelo-Silveira ... [et al.] -- Tiny molecular beacons for in vivo mRNA detection / Diana P. Bratu, Irina E. Catrina, and Salvatore A.E. Marras -- Delivery of molecular beacons for live-cell imaging and analysis of RNA / Antony K. Chen ... [et al.] -- Genetically-encoded fluorescent probes for imaging endogenous mRNA in living cells / Takeaki Ozawa and Yoshio Umezawa -- Visualization of induced RNA in single bacterial cells / Azra Borogovac and Natalia E. Broude -- Visualizing mRNAs in fixed and living yeast cells / Franck Gallardo and Pascal Chartrand -- In vivo visualization of RNA using the U1A-based tagged RNA system / Sunglan Chung and Peter A. Takizawa -- Visualizing endogenous mRNAs in living yeast using m-TAG, a PCR-based RNA aptamer integration method, and fluorescence microscopy / Liora Haim-Vilmovsky and Jeffrey E. Gerst -- Imaging mRNAs in living mammalian cells / Sharon Yunger and Yaron Shav-Tal -- Using the mRNA-MS2/MS2CP-FP system to study mRNA transport during Drosophila oogenesis / Katsiarina Belaya and Daniel St Johnston -- Genome-wide analysis of RNA extracted from isolated mitochondria / Erez Eliyahu, Daniel Melamed and Yoav Arava -- Analyzing mRNA localization to the endoplasmic reticulum via cell fractionation / Sujatha Jagannathan, Christine Nwosu and Christopher V. Nicchitta -- Isolation of mRNAs encoding peroxisomal proteins from yeast using a combined cell fractionation and affinity purification procedure / Gadi Zipor, Cecile Brocard and Jeffrey E. Gerst -- Profiling axonal mRNA transport / Dianna E. Willis and Jeffery L. Twiss -- RNA purification from tumor cell protrusions using porous polycarbonate filters / Jay Shankar and Ivan R. Nabi -- RNA-binding protein immunopurification-microarray (RIP-chip) analysis to profile localized RNAs / Alessia Galgano and André P. Gerber -- RaPID : an aptamer-based mRNA affinity purification technique for the identification of RNA and protein factors present in ribonucleoprotein complexes / Boris Slobodin and Jeffrey E. Gerst -- RIP : an mRNA localization technique / Sabarinath Jayaseelan ... [et al.] -- The dual use of RNA aptamer sequences for affinity purification and localization studies of RNAs and RNA-protein complexes / Scott C. Walker ... [et al.] -- Identifying and searching for conserved RNA localisation signals / Russell S. Hamilton and Ilan Davis -- Computational prediction of RNA structural motifs involved in post-transcriptional regulatory processes / Michal Rabani, Michael Kertesz, and Eran Segal.
  • 2014 Springer Protocols
    edited by Christina Waldsich.
    Predicting RNA Structure: Advances and Limitations -- Cis-Acting 5' Hammerhead Optimization for In Vitro Transcription of Highly Structured RNAs -- RNA Structural Analysis by Enzymatic Digestion -- A Mutate-and-Map Protocol for Inferring Base Pairs in Structured RNA -- Mapping RNA Structure In Vitro Using Nucleobase-Specific Probes -- Massively Parallel RNA Chemical Mapping with a Reduced Bias MAP-Seq Protocol -- Probing RNA Folding by Hydroxyl Radical Footprinting -- Monitoring Global Structural Changes and Specific Metal Binding Sites in RNA by In-Line Probing and Tb(III) Cleavage -- Chemical Probing of RNA in Living Cells -- A Chemogenetic Approach to Study the Structural Basis of Protein-Facilitated RNA Folding -- Applying UV Crosslinking to Study RNA-Protein Interactions in Multicomponent Ribonucleoprotein Complexes -- The Kinetics of Ribozyme Cleavage: A Tool to Analyze RNA Folding as a Function of Catalysis -- RNA Catalytic Activity as a Probe of Chaperone-Mediated RNA Folding -- Evaluation of RNA Chaperone Activity In Vivo and In Vitro Using Misfolded Group I Ribozymes -- RNA Conformational Changes Analyzed by Comparative Gel Electrophoresis -- Detecting RNA Tertiary Folding by Sedimentation Velocity Analytical Ultracentrifugation -- RNA Folding Dynamics Using Laser Assisted Single-Molecule Refolding -- RNA Refolding Studied by Light-Coupled NMR Spectroscopy -- Exploring RNA Oligomerization and Ligand Binding by Fluorescence Correlation Spectroscopy and Small Angle X-Ray Scattering.
  • 2016 Springer Protocols
    edited by Zdravka Medarova.
    Imaging functional nucleic acid delivery to skin / Roger L. Kaspar ... [et al.] -- In vivo magnetic resonance imaging of small interfering RNA nanodelivery to pancreatic islets / Ping Wang and Anna Moore -- Magnetic resonance spectroscopy of siRNA-based cancer therapy / Marie-France Penet ... [et al.] -- Targeted delivery with imaging assessment of siRNA expressing nanocassettes into cancer / Wei Chen and Lily Yang -- Analyses of tumor burden in vivo and metastasis ex vivo using luciferase-expressing cancer cells in an orthotopic mouse model of neuroblastoma / Frances L. Byrne, Joshua A. McCarroll, and Maria Kavallaris -- Indium-labeling of siRNA for small animal SPECT imaging / Steven Jones and Olivia Merkel -- Imaging of electrotransferred siRNA / Muriel Golzio and Justin Teissié -- Whole-body scanning PCR, a tool for the visualization of the in vivo biodistribution pattern of endogenous and exogenous oligonucleotides in rodents / Julien A. Boos and Iwan Beuvink -- siRNA nanoparticles for ultra-long gene silencing in vivo / Seung Koo Lee and Ching-Hsuan Tung -- Sensing miRNA : signal amplification by cognate RISC for intracellular detection of miRNA in live cells / Amol Kavishwar and Zdravka Medarova -- Molecular beacon-based microRNA imaging during neurogenesis / Jonghwan Lee and Soonhag Kim -- Hypoxia-responsive copolymer for siRNA delivery / Federico Perche ... [et al.] -- Controlling RNA expression in cancer using iron oxide nanoparticles detectable by MRI and in vivo optical imaging / Zdravka Medarova, Mustafa Balcioglu, and Mehmet V. Yigit -- Microvesicles : isolation, characterization for in vitro and in vivo procedures / Karmele Valencia and Fernando Lecanda -- Positive bioluminescence imaging of microRNA expression in small animal models using an engineered genetic-switch expression system, RILES / Patrick Baril and Chantal Pichon -- MicroRNA imaging in combination with diagnostic ultrasound and bubble liposomes for microRNA delivery / Yoko Endo-Takahashi ... [et al.].
  • 2013 Springer
    Kenneth A. Howard, editor.
    The Nobel Prize winning discovery that small interfering RNA can be utilised to control cellular gene expression has propelled the field of RNA interference (RNAi) to the forefront of biomedical science as a potential molecular medicine set to revolutionalise disease treatment. Harnessing the molecular mechanisms of RNAi and development of delivery technologies is crucial for its transformation into a therapeutic modality, this dependency is the focus of "RNA Interference from Biology to Therapeutics" that gives a comprehensive overview of RNAi biology and state-of-the-art delivery methods relevant to clinical translation of RNAi therapeutics. Key players and shapers in the fields of RNAi and delivery science have been assembled in a single volume to produce a truly unique interdisciplinary text, making it a "must-read" for both students and experts in, and at the interface of, RNAi, pharmaceutical science and medicine. An attractive feature is the "future perspectives" section within each chapter that allows global leaders the opportunity to express their views on the direction the field is moving. Topics covered in the book include miRNA biology and therapeutic exploitation, exosome delivery and clinical translation."RNA Interference from Biology to Therapeutics" is an up-to-the-minute, highly informative and invaluable text for those actively involved or interested in this fascinating and high-impact field.
  • 2014 Springer Protocols
    edited by M. Lucrecia Alvarez, Mahtab Nourbakhsh.
    Part I Structural RNA mapping -- Full-length characterization of transcribed genomic region -- Rapid mapping of RNA 3 and 5 ends -- Single nucleotide mapping of RNA 5 and 3 ends -- Analysis of RNA secondary structure -- Tertiary structure mapping of the Pri-miRNA miR-17-92 -- In situ hybridization detection of miRNA using LNA [trademark] oligonucleotides -- Quantification of miRNAs by a simple and specific qPCR method -- RNA isolation for small RNA next-generation sequencing from acellular biofluids -- Sequencing small RNA : introduction and data analysis fundamentals -- Measuring expression levels of small regulatory RNA molecules from body fluids and formalin-fixed, paraffin-embedded samples -- MicroRNA profiling in plasma or serum using quantitative RT-PCR -- MicroRNA profiling of exosomes isolated from biofluids and conditioned media -- Isolation of urinary exosomes for RNA biomarker discovery using a simple, fast, and highly scalable method -- Part II Functional RNA mapping -- Identification of actively translated mRNAs -- Mapping of internal ribosome entry sites (IRES) -- Mapping of protein binding RNA elements -- Purification of RNA-binding proteins -- De novo approach to classify protein-coding and noncoding transcripts based on sequence composition -- Computational methods to predict long noncoding RNA functions based on co-expression network -- MicroRNA biogenesis : dicing assay -- Faster experimental validation of microRNA targets using cold fusion cloning and a dual firefly-Renilla luciferase reporter assay -- Experimental validation of predicted mammalian microRNAs of mirtron origin -- A guide for miRNA target prediction and analysis using web-based applications -- Tapping microRNA regulation networks through integrated analysis of microRNA-mRNA high-throughput profiles -- miRWalk database for miRNA-target interactions -- A schematic workflow for collecting information about the interaction between copy number variants and microRNAs using existing resources -- SYBR green and TaqMan quantitative PCR arrays : expression profile of genes relevant to a pathway or a disease state -- Comprehensive meta-analysis of microRNA expression using a robust rank aggregation approach.
  • 2012 Springer
    Albrecht Bindereif, editor.
    1. RNA polymerases and transcription factors of trypanosomes / Arthur Günzl -- 2. SL RNA biogenesis in kinetoplastids: a long and winding road / Nancy R. Sturm, Jesse R. Zamudio, and David A. Campbell -- 3. Pre-mRNA splicing in trypanosoma brucei: factors, mechanisms, and regulation / Christian Preusser, Nicolas Jaé, Arthur Günzl, and Albrecht Bindereif -- 4. mRNA turnover in trypanosomes / Christine Clayton -- 5. tRNA biogenesis and processing / Jessica L. Spears, Mary Anne T. Rubio, Paul J. Sample, and Juan D. Alfonzo -- 6. rRNA biogensis in trypanosomes / Shulamit Michaeli -- 7. RNA editing in African trypanosomes: a U-ser's G-U-ide / H. Ulrich Göringer -- 8. The RNA interference pathway in trypanosoma brucei / Elisabetta Ullu, Nikolay G. Kolev, Rebecca L. Barnes, and Christian Tschudi -- 9. Translation in trypanosomatids -- 10. Mitochondrial translation in trypanosomatids / Dmitri A. Maslov and Rajendra K. Agrawal -- 11. RNA-Seq analysis of the transcriptome of trypanosoma brucei / Jan Mani, Kapila Gunasekera, and Isabel Roditi.
  • 2010 ScienceDirect
    Robert E. Farrell Jr.
    RNA and the cellular biochemistry revisited -- RNA isolation strategies -- The truth about tissues -- Going green : RNA and the molecular biology of plants -- Isolation of polyadenylated RNA -- Quality control for RNA preparations -- Resilient ribonucleases -- Stringency : conditions that influence nucleic acid structure -- Electrophoresis of RNA -- Photodocumentation and image analysis -- Northern analysis -- Nucleic acid probe technology -- Practical nucleic acid hybridization -- Principles of detection -- Quantifications of specific mRNAs by nuclease protection -- Analysis of nuclear RNA -- cDNA synthesis -- RT-PCR : a science and an art form -- Quantitative PCR techniques -- Functional genomics and transcript profiling -- High-throughput analysis of gene expression -- Non-array methods for global analysis of gene expression -- RNAi : take a RISC-role the dicer -- Genomes, transcriptomes, proteomes, and bioinformatics -- An RNA paradigm.
  • 2015 Springer Protocols
    edited by Peixuan Guo, Farzin Haque.
  • 2014 Springer Protocols
    edited by Jan Gorodkin, Walter L. Ruzzo.
    The existence of genes for RNA molecules not coding for proteins (ncRNAs) has been recognized since the 1950's, but until recently, aside from the critically important ribosomal and transfer RNA genes, most focus has been on protein coding genes. However, a long series of striking discoveries, from RNA's ability to carry out catalytic function, to discovery of riboswitches, microRNAs and other ribo-regulators performing critical tasks in essentially all living organisms, has created a burgeoning interest in this primordial component of the biosphere. However, the structural characteristics and evolutionary constraints on RNA molecules are very different from those of proteins, necessitating development of a completely new suite of informatic tools to address these challenges. In RNA Sequence, Structure, Function: Computational and Bioinformatic Methods, expert researchers in the field describe a substantial and relevant fraction of these methodologies from both practical and computational/algorithmic perspectives. Focusing on both of these directions addresses both the biologist interested in knowing more about RNA bioinformatics as well as the bioinformaticist interested in more detailed aspects of the algorithms. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results. Thorough and intuitive, RNA Sequence, Structure, Function: Computational and Bioinformatic Methods aids scientists in continuing to study key methods and principles of RNA bioinformatics.
  • 2010 Springer
    Volker A. Erdmann, Jan Barciszewski, editors.
    The key features of RNA silencing / Kuniaki Saito ... [et al.] -- Selected strategies for the delivery of siRNA in vitro and in vivo / Sandra D. Laufer ... [et al.] -- RNAi suppression and its application / Xiaoping Yi, Rui Lu -- Strategies to prevent siRNA-triggered cellular toxicity / Matthias Bauer -- RNAi in malignant brain tumors: relevance to molecular and translational research / Mitsutoshi Nakada ... [et al.] -- Silencing Huntington's disease gene with RNAi / Yu Zhang, Robert M. Friedlander -- Application of Dicer-substrate siRNA in pain research / Philippe Sarret ... [et al.] -- RNAi treatment of HIV-1 infection / Karin J. von Eije, Ben Berkhout -- Application of RNA interference to treat conditions associated with dysregulation of transient receptor potential vanilloid 1 channel / Vickram Ramkumar ... [et al.] -- Harnessing RNAi-based functional genomics to unravel the molecular complexity underlying skin pigment variation / Hsiang Ho ... [et al.] -- mRNA structure and its effects on posttranscriptional gene silencing / Stephen I. Rudnick, Veenu Aishwarya, Alan M. Gewirtz -- Antisense RNA-mediated regulation of the p53 tumor suppressor / Marianne Farnebo, Klas G. Wiman -- Antisense oligonucleotides: insights from preclinical studies and clinical trials / Doreen Kunze, Kai Kraemer, Susanne Fuessel -- What can the new hammerhead ribozyme structures teach us about design? / William G. Scott -- microRNA biogenesis and its impact on RNA interference / Stefanie Grund, Sven Diederichs -- MicroRNAs in epithelial antimicrobial immunity / Jun Liu ... [et al.] -- Emerging roles of long noncoding RNAs in gene expression and intracellular organization / Tetsuro Hirose -- Noncoding RNAs as therapeutic targets / Maciej Szymański, Jan Barciszewski -- Noncoding RNAs at H19/IGF2 locus: role in imprinting, gene expression, and associated pathologies / Nahalie Berteaux, Nathalie Spruyt, Eric Adriaenssens.
  • 2008 Springer Protocols
    edited by Sailen Barik.
  • 2011 Springer Protocols
    edited by Hiroaki Kodama, Atsushi Komamine.
    Overview of plant RNAi / Yuichiro Watanabe -- Caveat of RNAi in plants : the off-target effect / Muthappa Senthil-Kumar and Kirankumar S. Mysore -- Plant gateway vectors for RNAi as a tool for functional genomic studies / Toshiya Muranaka -- Heat-inducible RNAi for gene functional analysis in plants / Frederic Masclaux and Jean-Philippe Galaud -- Gene function analysis by artificial microRNAs in Physcomitrella patens / Basel Khraiwesh [and others] -- Virus-induced gene silencing in ornamental plants / Cai-Zhong Jiang, Jen-Chih Chen, and Michael Reid -- Local RNA silencing mediated by agroinfiltration / Jutta Maria Helm, Elena Dadami, and Kriton Kalantidis -- Direct transfer of synthetic double-stranded RNA into protoplasts of Arabidopsis thaliana / Ha-il Jung, Zhiyang Zhai, and Olena K. Vatamaniuk -- Detection of long and short double-stranded RNAs / Toshiyuki Fukuhara [and others] -- Quantitative stem-loop RT-PCR for detection of microRNAs / Erika Varkonyi-Gasic and Roger P. Hellens -- Large-scale sequencing of plant small RNAs / William P. Donovan, Yuanji Zhang, and Miya D. Howell -- Computational prediction of plant miRNA targets / Ying-Hsuan Sun [and others] -- Bisulfite sequencing for cytosine-methylation analysis in plants / Nazmul Haque and Masamichi Nishiguchi -- Using nuclear run-on transcription assays in RNAi studies / Basel Khraiwesh -- Proteomic analysis of RNA interference induced knockdown plant / Sang Yeol Lee and Kyun Oh Lee -- Comparative analysis of phosphoprotein expression using 2D-DIGE / Tomoya Asano and Takumi Nishiuchi.
  • 2008 Springer Protocols
    edited by Ren-Jang Lin.
    1. Isolation of a sequence-specific RNA binding protein, polypyrimidine tract binding protein using RNA affinity chromatography / Shalini Sharma -- 2. An affinity oligonucleotide displacement strategy to purify ribonucleoprotein complexes applied to human telomerase /Isabel Kurth, Gaël Cristofari, Joachim Lingner -- 3. RNA affinity tags for the rapid purification and investigation of RNAs and RNA-protein complexes / Scott C. Walker ... [et al.] -- 4. Assembly and glycerol gradient isolation of yeast spliceosomes containing transcribed or synthetic U6 SnRNA / Ken Dery, Shyue-Lee Yean, Ren-Jang Lin -- 5. Purification of ribonucleoproteins using peptide-elutable antibodies and other affinity techniques / Scott Stevens -- 6. CLIP: cross-linking and immunoprecipitation of in vivo RNA targets of RNA binding proteins / Kirk B Jensen, Robert B. Darnell -- 7. Quantitative analysis of protein-RNA interactions by gel mobility shift / Sean P. Ryder, Michael I. Recht, James R. Williamson -- 8. Monitoring assembly of ribonucleoprotein complexes by isothermal titration calorimetry / Michael I. Recht, Sean P. Ryder, James R. Williamson -- 9. Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome / A. Ozlem Tastan Bishop, ... [et al.] -- 10. In vivo analysis of ribonucleoprotein complexes using nucleotide analog interference mapping / Lara Weinstein Szewczak -- 11. T7 RNA polymerase mediated incorporation of 8-N3AMP into RNA for studying protein-RNA interactions / Rajesh Gaur -- 12. A simple crosslinking method, CLAMP, to map the sites of RNA contacting domains within a protein / Hiren Banerjee, Ravinder Singh -- 13. Proteins specifically modified with a chemical nuclease as probes of RNA-protein interaction / Oliver A. Ken, Andrew M. MacMillan -- 14. RNA-protein crosslink mapping using TEV protease / Ian A. Turner, ... [et al.] -- 15 Structural analysis of protein-RNA interactions with mass spectrometry / Mamuka Kvaratskhelia, Stuart F.J. Le Grice -- 16. Analysing RNA-protein cross-linking sites in unlabeled ribonucleoprotein complexes by mass spectrometry / Henning Urlaub, Eva Kühn-Hölsken, Reinhard Lührmann -- 17. In vitro selection of random RNA fragments to identify protein binding sites within large RNAs / Ulrich Stelzl, Knud H. Nierhaus -- 18. Immunoprecipitation analysis to study RNA-protein interactions in xenopus oocytes / Naoto Mabuchi, Kaoru Masuyama, Mutsuhito Ohno -- 19. Mapping the regions of RNase P catalytic RNA that are potentially in close contact with its protein cofactor / Phong Trang, Fenyong Liu -- 20. Quantification of microRNAs, splicing isoforms, and homologous mRNAs with the invader assay / Peggy S. Eis, Mariano Garcia-Blanco -- 21. Analysis of RNA structure and RNA-protein interactions in mammalian cells by use of terminal transferase-dependent PCR / Hsiu-Hua Chen, Jeanne LeBon, Arthur D. Riggs -- 22. Duplex unwinding and RNP remodeling with RNA helicases / Eckhard Jankowsky, Margaret E. Fairman -- 23. Preparation of efficient splicing extracts from whole cells, nuclei, and cytoplasmic fractions / Naoyuki Kataoka, Gideon Dreyfuss -- 24. Designing and utilization of siRNAs targeting RNA-binding proteins / Dong-Ho Kim, Mark Behlke, John J. Rossi -- 25. The use of saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins / Jane E. Jackman, Eric M. Phizicky.
  • 2015 Springer Protocols
    edited by Frank J. Schmidt.
    Identifying RNA recombination events and non-covalent RNA-RNA interactions with the molecular colony technique / Helena V. Chetverina and Alexander B. Chetverin -- RNA-directed recombination of RNA in vitro / Niles Lehman, Nilesh Vaidya, and Jessica A.M. Yeates -- RNA-RNA SELEX / B. Cho -- Identification of antisense RNA stem-loops that inhibit RNA-protein interactions using a bacterial reporter system / Kazuo Harada -- Transactivation of large ribozymes / Matthew B. Martin, Thomas L. Leeper, and Frank J. Schmidt -- Native purification and labeling of RNA for single molecule fluorescence studies / Arlie J. Rinaldi, Krishna C. Suddala, and Nils G. Walter -- Single molecule studies of RNA-RNA interactions / Dongmei Yu, Peiwu Qin,