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  • Conventional specimen preparation techniques for transmission electron microscopy of cultured cells / John J. Bozzola -- Processing biological tissues for ultrastructural study / José A. Mascorro and John J. Bozzola -- Processing plant tissues for ultrastructural study / John Kuo and Ivan Kuo -- Microwave-assisted processing and embedding for transmission electron microscopy / Paul Webster -- Ultramicrotomy for biological electron microscopy / Herbert K. Hagler -- Poststaining grids for transmission electron microscopy : conventional and alternative protocols / E. Ann Ellis -- Negative staining of thinly spread biological samples / J. Robin Harris -- Recent advances in high-pressure freezing : equipment- and specimen-loading methods / Kent L. McDonald ... [et al.] -- Cryo-ultramicrotomy : cryo-electron microscopy of vitreous sections / Dimitri Vanhecke, Luca Studer, and Daniel Studer -- Cell-free extract systems and the cytoskeleton : preparation of biochemical experiments for transmission electron microscopy / Margaret Coughlin, William M. Brieher, and Ryoma Ohi -- Electron microscopy/in situ hybridization tracking of DNA and RNA sequences at high resolution / Dusan Cmarko and Karel Koberna -- Correlative light and electron microscopy using immunolabeled resin sections / Heinz Schwarz and Bruno M. Humbel -- Cryosectioning fixed and cryoprotected biological material for immunocytochemistry / Paul Webster and Alexandre Webster -- F-TEM images : integrating fluorescence microscopy with transmission electron microscopy / Paul A. Sims and Jeff D. Hardin -- Quantitative immunoelectron microscopy : alternative ways of assessing subcellular patterns of gold labelling / Terry M. Mayhew -- Electron crystallography of membrane proteins / Hui-Ting Chou, James Evans, and Henning Stahlberg -- Cryo-electron microscopy of icosahedral virus particle / Wen Jiang and Wah Chiu -- 3D reconstructions of chromosomes with electron tomography / Peter Engelhardt -- Whole-mount immuno-electron tomography of chromosomes and cells / Peter Engelhardt ... [et al.] -- 3D cryo-transmission electron microscopy of cells and organelles / Michael Marko and Chyong-Ere Hsieh -- Electron energy-loss spectroscopy (EELS) as a tool for elemental analysis in biological specimens / Nadine Kapp ... [et al.] -- Conventional specimen preparation techniques for scanning electron microscopy of biological specimens / John J. Bozzola -- Variable pressure and environmental scanning electron microscopy : imaging of biological samples / Brendan J. Griffin -- Cryoplaning technique for visualizing the distribution of water in woody tissue by cryo-scanning electron microscopy / Yasuhiro Utsumi and Yuzou Sano -- X-ray microanalysis in the scanning electron microscope / Godfrield M. Roomans and Anca Dragomir -- Botanical SEM X-ray microanalysis in cryo-scanning electron microscopy / Beat Frey -- Static secondary ion mass spectrometry for biological and biomedical research / Nicholas P. Lockyer -- Using secondary ion mass spectrometry and multi-isotope imaging mass spectrometry in biological materials : application to higher plants / Nicole Grignon.
  • In a conceptually current, quick-reference, Question & Answer format, the second edition of Handbook of Practical Immunohistochemistry: Frequently Asked Questions continues to provide a comprehensive and yet concise state-of-the-art overview of the major issues specific to the field of immunohistochemistry. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 10,000 tumors and normal tissue to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. ℗ℓ Fully revised and updated from the first edition, the new features of the second edition include over 200 additional questions or revised questions with an IHC panel to answer each question; over 250 new color photos and illustrations; over 20 new useful biomarkers; hundreds of new references; several new chapters to cover phosphoproteins, rabbit monoclonal antibodies, multiplex IHC stains, overview of predictive biomarkers, and integration of IHC into molecular pathology; many new coauthors who are international experts in a related field; many updated IHC panels using Geisinger IHC data collected from over 10,000 tumors and normal tissues; and updated appendices containing detailed antibody information for both manual and automated staining procedures. ℗ℓ Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, Second Edition will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.

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