Books by Subject
Genetics
Displaying 201 to 300 of 338 matchesChoose A-Z
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- (Opinionated) guide to microarray dat...
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- Functional microbial genomics ..........
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- Molecular diagnostic PCR handbook ......
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- Single nucleotide polymorphisms ........
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Molecular diagnostic PCR handbook Springer
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Molecular genetics of mammalian cells ScienceDirect
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Molecular genetics of recombination 2007, Springer
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Molecular mechanisms of Axenfeld-Rieger syndrome 2005, SpringerIdentification of the gene involved in 4q25-linked Axenfeld-Rieger syndrome, PITX2 / Elena V. Semina -- Winged helix/forkhead transcription factors and Rieger syndrome / Darryl Y. Nishimura and Ruth E. Swiderski -- Rieger syndrome and PAX6 deletion / Ruth Riise -- The molecular and biochemical basis of Axenfeld-Rieger syndrome / Brad A. Amendt -- Role of PITX2 in the pituitary gland / Hoonkyo Suh ... [et al.] -- Expression and function of PITX2 in chick heart looping / Xueyan Yu, Shusheng Wang, and YiPing Chen -- The multiple roles of PITX2 in heart development / James F. Martin -- The role of PITX2 in tooth development / Brad A. Amendt -- PITX genes and ocular development / Elena V. Semina -- An overview of Axenfeld-Rieger syndrome and the anterior segment developmental disorders / Brad A. Amendt.
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Molecular toxicology protocols 2004, Springer ProtocolsAnalysis of K-RAS and P53 mutations in sputum samples / Weimin Gao and Phouthone Keohavong -- Allele-specific competitive blocker-PCR detection of rare base substitution / Barbara L. Parsons, Page B. McKinzie, and Robert H. Heflich -- Gel-based nonradioactive single-strand conformational polymorphism and mutation detection: limitations and solutions / Vibhuti Gupta ... [et al.] -- Detection and characterization of oncogene mutations in preneoplastic and early neoplastic lesions / Toshinari Minamoto -- Detection of DNA double-strand breaks and chromosome translocations using ligation-mediated PCR and inverse PCR / Michael J. Villalobos, Christopher J. Betti, and Andrew T.M. Vaughan -- Microsatellite instability: an indirect assay to detect defects in the cellular mismatch repair machinery / Anjana Saha, Narendra K. Bairwa, and Ramesh Bamezai -- Unscheduled DNA synthesis: a functional assay for global genomic nucleotide excision repair / Crystal M. Kelly and Jean J. Latimer -- Analysis of DNA repair using transfection-based host cell reactivation / Jennifer M. Johnson and Jean J. Latimer -- An immunoassay for measuring repair of ultraviolet photoproducts / Shirley McCready -- Analysis of DNA double-strand break repair by nonhomologous end joining in cell-free extracts from mammalian cells / Petra Pfeiffer ... [et al.] --The comet-FISH technique: a tool for detection of specific DNA damage and repair / Alexander Rapp, Michael Hausmann, and Karl Otto Greulich -- DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis / Nina Joshi and Stephen G. Grant -- Analysis of in vivo mutation in the Hprt and Tk genes of mouse lymphocytes / Vasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H. Heflich -- Quantifying in vivo somatic mutations using transgenic mouse model systems / Roy R. Swiger -- Methods for detecting somatic mutations in vitro: the human T-cell cloning assay selecting for HPRT mutants / Sai-Mei Hou -- Molecular analysis of mutations in the human HPRT gene / Phouthone Keohavong, Liqiang Xi, and Stephen G. Grant -- Simultaneous quantification of t(14;18) and HPRT exon 2/3 deletions in human lymphocytes / James C. Fuscoe -- The GPA in vivo somatic mutation assay / Stephen G. Grant -- Flow cytometric measurement of mutant T cells with altered expression of TCR: detecting somatic mutations in humans and mice / Seishi Kyoizumi, Yoichiro Kusunoki, and Tomonori Hayashi -- Mutation screening of the TP53 gene by temporal temperature gradient gel electrophoresis / Therese Sørlie ... [et al.] --³²P-postlabeling analysis of DNA adducts / David H. Phillips, Alan Hewer, and Volker M. Arlt -- Modification of the ³²P-postlabeling method to detect a single adduct as a single spot / Masako Ochiai, Takashi Sugimura, and Minako Nagao -- DNA isolation and sample preparation for quantification of adduct levels by accelerator mass spectrometry / Karen H. Dingley ... [et al.] -- Fluoroimaging-based immunoassay of DNA photoproducts in ultraviolet-B-irradiated tadpoles / Iovanna Pandelova, Stephen R. Hewitt, and John B. Hays -- Analysis of DNA strand cleavage at abasic sites / Walter A. Deutsch and Vijay Hegde -- Premature chromosome condensation in human resting peripheral blood lymphocytes for chromosome aberration analysis using specific whole-chromosome DNA hybridization probes / Pataje G.S. Prasanna and William F. Blakely -- Mutagen-induced chromatid breakage as a marker of cancer risk / Xifeng Wu, Yun-Ling Zheng, and T.C. Hsu -- Flow cytometric analysis of micronuclei in erythrocytes / Jan Grawé -- The comet assay: a sensitive genotoxicity test for the detection of DNA damage / Günter Speit and Andreas Hartmann -- Computerized image analysis software for the comet assay / R.C. Chaubey --Measuring recombination proficiency in mouse embryonic stem cells / Andrew J. Pierce and Maria Jasin -- Strategies for measurement of biotransformation enzyme gene expression / Marjorie Romkes and Shama C. Buch -- Genotyping technologies: application to biotransformation enzyme genetic polymorphism screening / Marjorie Romkes and Shama C. Buch -- TaqMan® fluorogenic detection system to analyze gene transcription in autopsy material / Kaori Shintani-Ishida, Bao-Li Zhu, and Hitoshi Maeda -- Development of quantitative reverse transcriptase PCR assays for measuring gene expression / Tony E. Godfrey and Lori A. Kelly -- Quantification of selective phosphatidylserine oxidation during apoptosis / James P. Fabisiak ... [et al.] -- Quantitative method of measuring of phosphatidylserine externalization during apoptosis using electron paramagnetic resonance spectroscopy and annexin-conjugated iron / James P. Fabisiak, Grigory G. Borisenko, and Valerian E. Kagan -- Detection of programmed cell death in cells exposed to genotoxic agents using a caspase activation assay / Michael E. Gehring and Patrick P. Koty.
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From destiny to freedom? : on human nature and liberal eugenics in the age of genetic manipulation /Michela Betta --Diagnostic knowledge in the genetic economy and commerce /Michela Betta --Body talk : genetic screening as a device of crime regulation /Richard Hil, Richard Hindmarsh --Genetic testing and human genetic databases /Astrid H. Gesche --Theimperative of the "new genetics" : challenges for ethics, law, and social policy /David Weisbrot --Insurance and genetics : regulating a private market in the public interest /David Weisbrot, Brian Opeskin --Thesocial imperative for community genetic screening : an Australian perspective /MaryAnne Aitken, Sylvia Metcalfe --Genetically transformed healthcare : healthy children and parents /Enzo A. Palombo, Mrinal Bhave --TheAustralian law reform inquiry into genetic commission testing - a worker's perspective /Susan Pennicuik --Genetic information and the Australian labour movement /Suzanne Jamieson --Protecting the vulnerable : genetic testing and screening for parentage, immigration, and aboriginality /Astrid H. Gesche --Essentially whose? : genetic testing and the ownership of genetic information /Lyn Turney --Self-knowledge and self-care in the age of genetic manipulation /Michela Betta.
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Morphogenesis and pathogenicity in fungi 2012, Springer
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Morphology, shape and phylogeny 2002, CRCnetBASE
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mRNA processing and metabolism Springer ProtocolsGene expression analysis of messenger RNP complexes / Luiz O.F. Penalva, Scott A. Tenenbaum, and Jack D. Keene -- Using the lambdaN peptide to tether proteins to RNAs / Julie Baron-Benhamou ... [et al.] -- An efficient system for cap- and poly(A)-dependent translation in vitro / Yuri V. Svitkin and Nahum Sonenberg -- A poly(A) tail-responsive in vitro system for cap- or IRES-driven translation from HeLa cells / Christian Thoma, Antje Ostareck-Lederer and Matthias W. Hentze -- Assessing messenger RNA decapping in cellular extracts / Naomi Bergman ... [et al.] -- Analysis of RNA exonucleolytic activities in cellular extracts / Devi Mukherjee ... [et al.] -- Application of ligation-mediated reverse transcription polymerase chain reaction to the identification of in vivo endonuclease-generated messenger RNA decay intermediates / Mark N. Hanson and Daniel R. Schoenberg -- Biochemical dissection of RNA silencing in plants / Guiliang Tang and Phillip D. Zamore -- RNA interference of mRNA processing factors in drosophila S2 cells / Alicia M. Celotto and Brenton R. Graveley -- RNA interference by short hairpin RNAs expressed in vertebrate cells / Gregory J. Hannon and Douglas S. Conklin.Using chromatin immunoprecipitation to map cotranscriptional mRNA processing in saccharomyces cerevisiae / Michael-Christopher Keogh and Stephen Buratowski -- Identifying phosphoCTD-associating proteins / Hemali P. Phatnani and Arno L. Greenleaf -- Imaging alternative splicing in living cells / Eric J. Wagner ... [et al.] -- Tobramycin affinity tag purification of spliceosomes / Klaus Hartmuth, Hans-Peter Vornlocher and Reinhard Lührmann -- Using single-strand conformational polymorphism gel electrophoresis to analyze mutually exclusive alternative splicing / Alicia M. Celotto and Brenton R. Graveley -- Methods for the analysis of adenosine-to-inosine editing in RNA / Zuo Zhang and Gordon G. Carmichael -- Assaying nuclear messenger RNA export in human cells / Bryan R. Cullen -- RNA unwinding assay for DExD/H-Box RNA helicases / Stephanie S. Tseng-Rogenski and Tien-Hsien Chang -- Approaches for monitoring nuclear translation / Francisco J. Iborra, Dean A. Jackson, and Peter R. Cook -- Immunopurification and analysis of protein and RNA components of mRNP in mammalian cells / Fabrice Lejeune and Lynne E. Maquat --
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Multigenic and induced systemic resistance in plants 2006, Springer
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Multiple testing procedures with applications to genomics 2008, Springer
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Myc/Max/Mad transcription factor network 2006, Springer
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Neurobehavioral genetics. 2nd ed. 2007, CRCnetBASEChapter 1. A History of Behavior Genetics / Stephen C. Maxson -- Chapter 2. Developmental Neurobehavioral Genetics: Development as Explanation / Gilbert Gottlieb -- Chapter 3. Some Basics, Mendelian Traits, Polygenic Traits, Complex Traits / Byron C. Jones -- Chapter 4. An Introduction to Quantitative Genetics / Wim E. Crusio -- Chapter 5. From QTL Detection to Gene Identification / Marie-Pierre Moisan -- Chapter 6. Gene Expression / Richard A. Radcliffe -- Chapter 7. Bioinformatics of Behavior / Elissa J. Chesler -- Chapter 8. Congenic and Consomic Strains / Lorraine Flaherty and Valerie Bolivar -- Chapter 9. Animal Resources in Behavioral Neurogenetics / Jean-Michel Lassalle -- Chapter 10. Sample Size Requirements for Experiments on Laboratory Animals / Douglas Wahlsten -- Chapter 11. The Role of Association Studies in Psychiatric Disorders / Nicolas Ramoz and Philip Gorwood -- Chapter 12. Family and Twin Methods / Keith E. Whitfield and Tracy L. Nelson -- Chapter 13. Gene-Environment Interactions / Byron C. Jones and Leslie C. Jones -- Chapter 14. And Now it Starts to Get Interesting: Gene-Gene Interactions / Yamima Osher -- Chapter 15. Schizophrenia: Study of a Genetically Complex Phenotype / Michael F. Pogue-Geile and Irving I. Gottesman -- Chapter 16. Genetics of Major Affective Disorders / Wade Berrettini -- Chapter 17. Pedigree Analyses and the Study of Chimpanzee (Pan troglodytes) Personality and Subjective Well-Being / Alexander Weiss and James E. King -- Chapter 18. The Elusive World of Personality Genes: Cherchez le Phenotype / Richard P. Ebstein, Rachel Bachner-Melman, Jonathan Benjamin, and Robert H. Belmaker -- Chapter 19. Aggression: Concepts and Methods Relevant to Genetic Analyses in Mice and Humans / Stephen C. Maxson and Andrew Canastar -- Chapter 20. Genetic Analysis of Emotional Behaviors Using Animal Models / André Ramos and Pierre Mormède -- Chapter 21. Genetic Analysis of Food Search Behavior in the Fruit Fly (Drosophila melanogaster) / Amsale T. Belay and Marla B. Sokolowski -- Chapter 22. Genetic and Molecular Analyses of Drosophila
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Neurogenetics 2006, CRCnetBASE
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New high throughput technologies for DNA sequencing and genomics. 1st ed. 2007, ScienceDirectOverview: developments in DNA sequencing / Keith R. Mitchelson ... [et al.] -- Chip capillary electrophoresis and total genetic analysis systems / Quiang Xiong and Jing Cheng -- Comparative sequence analysis by MALDI-TOF mass spectrometry: utilizing the known to discover the new / Mathias Ehrich, Franz Hillenkamp, and Dirk van den Boom -- Advances in dye-nucleotide conjugate chemistry for DNA sequencing / Shiv Kumar and Carl W. Fuller -- The 454 life sciences picoliter sequencing system / Marcel Margulies ... [et al.] -- An integrated system for DNA sequencing by synthesis / John R. Edwards, Dae Hyun Kim, and Jingyue Ju -- Single-molecule fluorescence microscopy and its applications to single-molecule sequencing by cyclic synthesis / Benedict Hebert and Ido Braslavsky -- Rapid DNA sequencing by direct nanoscale reading of nucleotide bases on individual DNA chains / James Weifu Lee and Amit Meller -- A single molecule system for whole genome analysis / Shiguo Zhou, Jill Herschleb, and David C. Schwartz -- Sequencing aided by mutagenesis facilitates the de novo sequencing of megabase DNA fragments by short read lengths / Jonathan M. Keith ... [et al.] -- Genome sequencing and assembly / Annette McGrath -- Valid recovery of nucleic acid sequence information from high contamination risk samples: ancient DNA and environmental DNA / George A. Kowalchuk ... [et al.].
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Nitric oxide, cell signaling, and gene expression 2006, CRCnetBASE
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Nonhuman DNA typing 2008, CRCnetBASEIntroduction to forensic science and DNA / Heather Miller Coyle -- Collection and preservation of biological evidence from the crime scene / Henry C. Lee -- Techniques of DNA fingerprinting / John Schienman -- Forensic canine STR analysis / Burkhard Berger, Cordula Eichmann, Walther Parson -- STR based forensic analysis of field samples from domestic and exotic cats / Marilyn A. Menotti-Raymond, Victor A. David, Stephen J. O'Brien -- Overview of insect evidence in forensic science / Heather Miller Coyle -- Use of forensic DNA typing in wildlife investigations / Richard M. Jobin -- DNA testing of animal evidence : case examples and method development / En-Uei Jiang, W. A. van Haeringen, L. H. P. van de Goor, Pero Dimsoski, Heather Miller Coyle -- Basics of forensic fungi /; Khudooma Said Al Na'imi -- Soil DNA typing in forensic science / Jonathan Hill, Linda Strausbaugh, Joerg Graf -- DNA profiling : the ability to predict an image from a DNA profile / Holly Long -- Appendix A: Brief history of forensic serology and DNA / Peter Bilous.
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Nonlinear physics of DNA. 2nd, rev. ed. 2004, Wileych. 1. DNA Structure, p. 1-17 -- ch. 2. DNA Dynamics, p. 19-39 -- ch. 3. DNA Function, p. 41-48 -- ch. 4. Linear Theory of DNA, p. 49-79 -- ch. 5. Nonlinear Theory of DNA: Ideal Dynamical Models, p. 81-114 -- ch. 6. Nonlinear Theory of DNA: Non-Ideal Models, p. 115-132 -- ch. 7. Nonlinear Theory of DNA: Statistics of Nonlinear Excitations, p. 133-150 -- ch. 8. Experimental Tests of DNA Nonlinearity, p. 151-158 -- ch. 9. Nonlinearity and Function, p. 159-167.
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Nuclear pre-mRNA processing in plants 2008, Springer
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Nuclear transfer protocols Springer ProtocolsCloning of exotic/endangered species: (desert bighorn sheep) / James "Buck" Williams ... [et al.] -- Handmade somatic cell cloning in cattle / Gábor Vatja, Ian M. Lewis, and R. Tayfur Tecirlioglu -- Genetic modification of sheep by nuclear transfer with gene-targeted somatic cells / A. John Clark, Sarah Burl, and Chris Denning -- Transgenesis using nuclear transfer in goats / Anthoula Lazaris ... [et al.] -- Nuclear reprogramming : an overview / Takashi Tada -- Expression of imprinted genes in cloned mice / Takashi Kohda, Fumitoshi Ishino, and Atsuo Ogura -- Nuclear remodeling assay in xenopus egg extract / Koichi Gonda and Nobuaki Kikyo -- In vitro reprogramming of nuclei and cells / Anne-Mari Håkelien ... [et al.] -- Techniques for nuclear transfer to mouse embryonic stem cells / Danielè Pralong and Paul J. Verma -- Gene expression and methylation patterns in cloned embryos / Christine Wrenzycki ... [et al.] -- Are there any normal clones? / Ian Wilmut -- Future and applications of cloning / Alan O. Trounson.Origin and progress of nuclear transfer in nonmammalian animals / Marie A. Di Berardino -- In vitro maturation and embryo production in cattle / Marc-André Sirard and Karine Coenen -- Activation of oocytes after nuclear transfer / Zoltán Macháty -- Culture medium preferences of pre-implantation cloned mouse embryos / Björn Heindryckx, Josiane Van der Elst, and Marc Dhont -- Maintenance of pregnancy in pigs with limited viable embryos / Tim King and Paul A. De Sousa -- Health and neonatal care of bovine clones / Jacqui T. Forsyth and David N. Wells -- Optimization of procedures for cloning by somatic cell nuclear transfer in mice / Young Gie Chung, Shaorong Gao, and Keith E. Latham -- Production of cloned calves using roscovitine-treated adult somatic cells as donors / Kazuchika Miyoshi, Sezen Arat, and Steven L. Stice -- A double nuclear transfer technique for cloning pigs / Irina A. Polejaeva, Shawn Walker, and Keith Campbell -- Nuclear transfer in nonhuman primates / Shoukhrat M. Mitalipov and Don P. Wolf --
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Nucleic acid testing for human disease 2006, CRCnetBASE
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Nucleic acids hybridization Springer
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Nutrient-gene interactions in cancer 2006, CRCnetBASE
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Nutrigenetics and nutrigenomics 2004, Karger
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Nutrigenomics 2005, CRCnetBASE
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Oligonucleotide synthesis Springer ProtocolsSynthesis of DNA using a new two-step cycle / Douglas J. Dellinger ... [et al.] -- RNA synthesis using 2'-O-(tert-butyldimethylsilyl) protection / Brian S. Sproat -- RNA oligonucleotide synthesis via 5'-silyl-2'-orthoester chemistry / Stephanie A. Hartsel... [et al.] -- Dimethylthiarum disulfide: new sulfur transfer reagent in phosphorothioates oligonucleotide synthesis -- Zhiwei Wang, Quanlai Song, and Yogesh H. Sanghvi -- Assay for evaluating ribonuclease H-mediated degradation of RNA-antisense oligonucleotide duplexes / Annie Galarneau ... [et al.] -- Di- and oligonucleotide synthesis using H-phosphonate chemistry / Jacek Stawinski and Roger Strömberg -- Solid-phase synthesis of circular oligonucleotides / Enrique Pedroso ... [et al.] -- Locked nucleic acid synthesis / Henrik M. Pfundheller ... [et al.] -- Synthesis of DNA mimics representing HypNA-pPNA hetero-oligomers / Vladimir A. Efimov and Oksana G. Chakhmakhcheva -- Base-modified oligonucleotides with increased duplex stability: pyrazolo[3,4-d] pyrimidines replacing purines / Frank Seela ... [et al.] --Introduction of hypermodified nucleotides in RNA / Darrell R. Davis and Ashok C. Bajji -- Chemical methods for peptide-oligonucleotide conjugate synthesis / Dmitry A. Stetsenko and Michael J. Gait -- Biotin labeled oligonucleotides with extraordinarily long tethering arms / A. Michael Morocho ... [et al.] -- Universal labeling chemistry for nucleic acid detection on DNA chips / Ali Laayoun ... [et al.] -- Postsynthetic functionalization of triple helix-forming oligonucleotides / Alexandre S. Boutorine and Jian-Sheng Sun -- Fluorescence-based on-line detection as an analytical tool in RNA electrophoresis / Ute Scheffer and Michael Göbel -- Design and optimization of molecular beacon real-time polymerase chain reaction assays / Jacqueline A. M. Vet and Salvatore A. E. Marras -- High-thoughput production of optimized primers (fimers) for whole-genome direct sequencing / Nikolai Polushin ... [et al.] -- Nonenzymatic template directed RNA synthesis / Marcus Hey and Michael Göbel -- DNAse I footprinting of small molecule binding sites on DNA / Christian Bailly ... [et al.] --Gene targeting using peptide nucleic acid / Peter E. Nielsen -- Nucleic acid library construction using synthetic DNA constructs / Hani S. Zaher and Peter J. Unrau -- In vitro selection from combinatorial nucleic acid libraries / Andres Jäschke -- In vitro selection procedures for identifying DNA and RNA aptamers targeted to nucleic acids and proteins / Eric Dausse ... [et al.] -- Short double-stranded ribonucleic acid as inhibitor of gene expression by the interference mechanism / Jean-Rémi Bertrand, Andrei Maksimenko, and Claude Malvy.
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Omics perspective on cancer research 2010, Springer
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Oncogenomics handbook 2005, Springer
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Organelle genetics 2012, Springer
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Organelles, genomes, and eukaryote phylogeny 2004, CRCnetBASE
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Origin of group identity 2009, SpringerAn overview : identity from bacteria to belief -- The prokaryotes : viruses, hyperparasites and the origin of group identity -- Sensory systems (light, odor, pheromones) in communities of oceanic microbes -- Subjugation of the individual : prokaryotic group living : blooms, slime and mats -- Animal group identity : from slime to worms, emergence of the brain -- Group identity in aquatic animals : learning to belong -- Development of tetrapod group identity, the smell of self -- Origin of primate group identity : vision and the great ERV invasion -- Human group identity : language and a social mind.
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A brief overview of molecular genetics -- An overview of information theory -- More on molecular genetics -- More on information theory -- An outline of error-correcting codes -- DNA is an ephemeral memory -- A toy living world -- Subsidiary hypothesis, nested system -- Soft codes -- Biological reality conforms to the hypotheses -- Identification of genomic codes.
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Paramecium 2008, CRCnetBASEMating types in paramecium -- General description of the protista and of paramecium -- Methods of studying genetic processes in organisms of the paramecium Aurelia species group -- The determination of mating types in paramecium -- Symbionts and mitochondria of paramecium -- Determination of i-antigens -- Micronuclei and macronuclei -- Ribosomal RNA and DNA -- Cortical morphogenesis -- Behavior -- Epigenetics.
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PCR mutation detection protocols Springer Protocols
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PCR mutation detection protocols. 2nd ed. 2011, Springer ProtocolsConformation-Sensitive Capillary Electrophoresis / Emma Jane Ashton -- Conformation Sensitive Gel Electrophoresis / Marian Hill -- Denaturing HPLC for Mutation Screening / Mike Mitchell, Jacqueline Cutler -- In Situ Detection of Human Papillomavirus DNA After PCR-Amplification / Gerard J. Nuovo -- LATE-PCR and Allied Technologies: Real-Time Detection Strategies for Rapid, Reliable Diagnosis from Single Cells / Kenneth E. Pierce, Lawrence J. Wangh -- Long-PCR Amplification of Human Genomic DNA / Stephen Keeney -- Human Papilloma Virus Strain Detection Utilising Custom-Designed Oligonucleotide Microarrays / Duncan Ayers, Mark Platt, Farzad Javad, Philip J. Day -- Multiplex Ligation-Dependent Probe Amplification (MLPA®) for the Detection of Copy Number Variation in Genomic Sequences / Petra G. Os, Jan P. Schouten -- Screening for Genomic Rearrangements by Multiplex PCR/Liquid Chromatography / Claude Houdayer, Catherine Dehainault, Marion Gauthier-Villars, Dominique Stoppa-Lyonnet -- Mutation Surveyor: Software for DNA Sequence Analysis / Jayne A. Minton, Sarah E. Flanagan, Sian Ellard -- Non-invasive Prenatal Diagnosis / Cathy Meaney, Gail Norbury -- Automated DNA Sequencing / Yvonne Wallis, Natalie Morrell -- Phylogenetic Microarrays for Cultivation-Independent Identification and Metabolic Characterization of Microorganisms in Complex Samples / Alexander Loy, Michael Pester, Doris Steger -- Prenatal Detection of Chromosome Aneuploidy by Quantitative-Fluorescence PCR / Kathy Mann, Erwin Petek, Barbara Pertl -- Use of Robotics in High-Throughput DNA Sequencing / Stephen Keeney -- Detection of Factor V Leiden and Prothrombin c.20210G>A Allele by Roche Diagnostics LightCycler® / Peter C. Cooper -- RT-PCR for the Detection of Translocations in Bone and Soft Tissue Tumours in Formalin-Fixed Paraffin-Embedded Tissues / Ann Williams, D. Chas Mangham -- Detection of Minimal Residual Disease in Leukaemia by RT-PCR / Joanne Mason, Mike Griffiths -- Mutation Detection by Southern Blotting / Gillian Mellars, Keith Gomez.
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PCR protocols. 2nd ed. Springer Protocols
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PCR protocols. 3rd ed. 2011, Springer ProtocolsSetup of a PCR Laboratory / Zaheer Khan -- Long-Range PCR with a DNA Polymerase Fusion / Holly H. Hogrefe, Michael C. Borns -- Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures / Bin Yin -- Lariat-Dependent Nested PCR for Flanking Sequence Determination / Daniel J. Park -- CODEHOP PCR and CODEHOP PCR Primer Design / Jeannette P. Staheli, Richard Boyce, Dina Kovarik, Timothy M. Rose -- Sequencing of Difficult DNA Regions by SAM Sequencing / Keith R. Mitchelson -- A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis / Kazuki Kurimoto, Mitinori Saitou -- Quantitation of MicroRNAs by Real-Time RT-qPCR / Caifu Chen, Ruoying Tan, Linda Wong, Richard Fekete, Jason Halsey -- High-Throughput SuperSAGE / Hideo Matsumura, Kentaro Yoshida, Shujun Luo, Detlev H. Krüger, Günter Kahl, Gary P. Schroth, Ryohei Terauchi -- Deep Cap Analysis of Gene Expression / Junpei Kurosawa, Hiromi Nishiyori, Yoshihide Hayashizaki -- Linking Emulsion PCR Haplotype Analysis / James G. Wetmur, Jia Chen -- PAP-LMPCR: An Improved, Sequence-Selective Method for the In Vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure / Richard Ingram, Arthur Riggs, Constanze Bonifer -- The Many Faces of MLPA / Thomas Ohnesorg, Erin Turbitt, Stefan J. White -- Assessing Gene-Specific Methylation Using HRM-Based Analysis / Ee Ming Wong, Alexander Dobrovic -- Alu PCR / Maurizio Cardelli -- Asynchronous PCR / Caifu Chen, David Ruff, Jason Halsey -- Novel Applications of PCR Through the Use of DNA Substrates / Stuart M. Wilson -- Enhanced Solid Phase PCR for Increased Loading of Amplicon onto Solid Support / Daniel J. Park -- Application of Blocking Oligonucleotides to Improve Signal-to-Noise Ratio in a PCR / Hege Vestheim, Bruce E. Deagle, Simon N. Jarman -- Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis / Yue-Hua Xiao, Yan Pei -- Ribosome Display: A Technology for Selecting and Evolving Proteins from Large Libraries / Birgit Dreier, Andreas Plückthun -- GLOBE: Analysis of DNA-Protein Interaction Analysis / Takaaki Kojima, Hideo Nakano -- PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues / Yimin Sun, Jing Cheng, Keith R. Mitchelson -- Nucleotide Exchange and Excision Technology DNA Shuffling and Directed Evolution / Janina Speck, Sabine C. Stebel, Katja M. Arndt, Kristian M. Müller.
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Pharmacogenomics Springer Protocols
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Pharmacogenomics and personalized medicine 2008, Springer ProtocolsChallenges, opportunities, and evolving landscapes in pharmacogenomics and personalized medicine : an industry perspective / Nadine Cohen and Theresa Frangiosa -- Implementation of pharmacogenomic sample collection in clinical trials / Deborah Sokol Ricci and Monique Franc -- Pharmacogenomics : the regulatory environment and labeling implications / Myong-Jin Kim ... [et al.] -- Applications of pharmacogenomics in drug discovery / Duncan McHale -- Applications of pharmacogenomics in clinical trials / Monique Franc -- Pharmacogenomics applications in drug metabolism : from genotyping to drug label-challenges? / Ann K. Daly -- The genetics of adverse drug reactions : promises and problems / Martin Armstrong -- Strategies and resources for marker selection and genotyping in genetic association studies / Nicole Soranzo, Dong-Jing Fu, and Qingqin S. Li -- Study design and statistical issues in pharmacogenetics research : from candidate genes to genome-wide screens / Nicholas J. Schork, Nathalie Malo, and Eric J. Topol -- Holy SNP, Batman! / Reyna Favis -- Predictive biomarker classifiers in the design of pivotal clinical trials / Richard Simon -- Translation of biomarkers into clinical utility / William L. Trepicchio and George Mulligan -- Pharmacogenomic study feasibility assessment and pharmaceutical business decision-making / Monique Franc and Theresa Frangiosa -- Co-development of drugs and pharmacogenomics-based diagnostics in oncology / Jeffrey S. Ross -- Pharmacogenomics applications in epilepsy / Chantal Depondt -- Pharmacogenomics in Alzheimer's disease / Ramón Cacabelos -- Pharmacogenomics applications in psychiatric disorders / Todd Lencz and Anil K. Malhotra -- Pharmacogenomics in HIV disease / Amalio Telenti -- Pharmacogenomics and cardiovascular drugs / Gérard Siest ... [et al.] -- Pharmacogenomic applications in children / Susan F.A. Grant and Hakon Hakonarson -- Pharmacogenomics of rare and monogenic disorders / Paul D. Maher.
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Pharmacogenomics in drug discovery and development 2008, Springer Protocols
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Phospho-proteomics 2009, Springer ProtocolsA high-resolution two dimensional gel- and pro-Q DPS-based proteomics workflow for phosphoprotein identification and quantitative profiling / Ganesh K. Agrawal and Jay J. Thelen -- Differential phosphoprotein labelling (DIPPL) using ³²P and ³³P / Aviva M. Tolkovsky and Andreas Wyttenbach -- Identification of oxidative stress-induced tyrosine phosphorylated proteins by immunoprecipitation and mass spectrometry / Ma Carmen Duran, Hong-Lin Chan, and John F. Timms -- Enrichment and characterization of phosphopeptides by immobilized metal affinity chromatography (IMAC) and mass spectrometry / Tine E. Thingholm and Ole N. Jensen -- The use of titanium dioxide micro-columns to selectively isolate phosphopeptides from proteolytic digests / Tine E. Thingholm and Martin R. Larsen -- Enrichment and separation of mono- and multiply phosphorylated peptides using sequential elution from IMAC prior to mass spectrometric analysis / Tine E. Thingholm , Ole N. Jensen, and Martin R. Larsen -- Use of stable isotope labeling by amino acids in cell culture (SILAC) for phophotyrosine protein identification and quantitation / Guoan Zhang and Thomas A. Neubert -- Hydrophilic interaction chromatography for fractionation and enrichment of the phosphoproteome / Dean E. McNulty and Roland S. Annan -- SILAC for global phosphoproteomic analysis / Genaro Pimienta, Raghothama Chaerkady, and Akhilesh Pandey -- Quantitative phospho-proteomics based on soluble nanopolymers / Anton Iliuk and W. Andy Tao -- Profiling the tyrosine phosphorylation state using SH2 domains / Kevin Dierck ... [et al.] -- An overview of the qualitative analysis of phosphoproteins by mass spectrometry / Philip R. Gafken -- The analysis of phosphoproteomes by selective labelling and advanced mass spectrometric techniques / Angela Amoresano ... [et al.] -- On-line liquid chromatography electron capture dissociation for the characterization of phosphorylation sites in proteins / Steve M.M. Sweet and Helen J. Cooper -- Quantification of protein phosphorylation by [mu]LC-ICP-MS / Ralf Krüger, Nico Zinn, and Wolf D. Lehmann -- Reverse-phase diagonal chromatography for phosphoproteome research / Kris Gevaert and Joël Vandekerckhove -- Chemical tagging strategies for mass spectrometry-based phospho-proteomics / Alexander Leitner and Wolfgang Lindner -- Antibody array platform to monitor protein tyrosine phosphorylation in mammalian cells / Alicia S. Chung and Y. Eugene Chin -- Protein tyrosine kinase characterization based on fully automated synthesis of (phospho)peptide arrays in microplates / W. Carl Saxinger -- Kinome profiling using peptide arrays in eukaryotic cells / Kaushal Parikh, Maikel P. Peppelenbosch, and Tita Ritsema -- ProMoST : a tool for calculating the pI and molecular mass of phosphorylated and modified proteins on two-dimensional gels / Brian D. Halligan -- Kinase-specific preiction of protein phosphorylation sites / Martin L. Miller and Nikolaj Blom -- Reconstructing regulatory kinase pathways from phosphopeptide data : a bioinformatics approach / Lawrence G. Puente, Robin E.C. Lee, and Lynn A. Megeney.
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Phylogenomics 2008, Springer ProtocolsFrom gene-scale to genome-scale phylogenetics : the data flood in, but the challenges remain / Antonis Rokas and Stylianos Chatzimanolis -- Phylogenomic analysis of chromosome sorting and painting / Roscoe Stanyon and Gary Stone -- FISH for mapping single copy genes / Terje Raudsepp and Bhanu P. Chowdhary -- Construction of radiation hybrid panels / John E. Page and William J. Murphy -- Survey sequencing and radiation hybrid mapping to construct comparative maps / Christophe Hitte ... [et al.] -- Construction of high-resolution comparative maps in mammals using BAC-end sequences / Denis M. Larkin and Harris A. Lewin -- Amniote phylogenomics : testing evolutionary hypotheses with BAC library scanning and targeted clone analysis of large-scale sequences from reptiles / Andrew M. Shedlock, Daniel E. Janes, and Scott V. Edwards -- Comparative physical mapping : universal overgo hybridization probe design and BAC library hybridization / James W. Thomas -- Phylogenomic resources at the UCSC Genome Browser / Kate Rosenbloom ... [et al.] -- Computational tools for the analysis of rearrangements in mammalian genomes / Glenn Tesler and Guillaume Bourque -- Computational reconstruction of ancestral DNA sequences / Mathieu Blanchette ... [et al.] -- Sequencing and phylogenomic analysis of whole mitochondrial genomes of animals / Rafael Zardoya and Mónica Suárez -- Retroposons : genetic footrpints on the evolutionary paths of life / Hidenori Nishihara and Norihiro Okada -- LINE-1 elements : analysis by FISH and nucleotide sequences / Paul D. Waters ... [et al.] -- Identification of cryptic sex chromosomes and isolation of X- and Y-borne genes / Paul D. Waters ... [et al.]
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Phytochemicals 2006, CRCnetBASE
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Phytochemistry in the genomics and post-genomics eras. 1st ed. 2002, ScienceDirect
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Pichia protocols. 2nd ed. Springer ProtocolsIntroduction : distinction between Pichia pastoris and other expression systems / James M. Cregg -- Vectors and strains for expression / Joan Lin-Cereghino and Geoff P. Lin-Cereghino -- DNA-mediated transformation / James M. Cregg -- Rational design and optimization of fed-batch and continuous fermentation / Wenhui Zhag, Mehmet Inan, and Michael M. Meagher -- Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) protein (Na-ASP1) / Mehmet Inan ... [et al.] -- Purification of the N- and C-terminal subdomains of recombinant heavy chain fragment C of botulinum neurotoxin serotype C / Jicai Huang ... [et al.] -- Rapid screening of chromatography resins for the purification of proteins / Sandra E. Ríos, Erin M. Giaccone, and Tillman U. Gerngross -- Characterization of O-linked saccharides on glycoproteins / Roger K. Bretthauer -- Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using glycoswitch plasmids / Wouter Vervecken ... [et al.] -- N-linked glycan characterization of heterologous proteins / Huijan Li ... [et al.] -- Heavy labeling of recombinant proteins / Eric Rodriguez -- Selenomethionine labeling of recombinant proteins / Anna M. Larsson and T. Alwyn Jones -- Selective isotopic labeling of recombinant proteins using amino acid auxotroph strains / James W. Whittaker -- Classical genetics / Ilya Tolstorukov and James M. Cregg -- Identification of pexophagy genes by restriction enzyme-mediated integration / Laura A. Schroder, Benjamin S. Glick, and William A. Dunn, Jr. -- Characterization of protein-protein interactions : application to the understanding of peroxisome biogenesis / Sebastien Leon ... [et al.] -- Localization of proteins and organelles using fluorescence microscopy / Jean-Claude Farre ... [et al.] -- Fluorescence microscopy and thin-section electron microscopy / Benjamin S. Glick.
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Plant chromosome engineering 2011, Springer ProtocolsRecent advances in plant transformation -- Engineering the plastid genome of nicotiana sylvestris, a diploid model species for plastid genetics -- Homologous recombination in plants : An antireview -- Chromosome painting for plant biotechnology -- Plant B chromosomes -- Telomere truncation in plants -- Engineered plant minichromosomes -- Method for bxb1-mediated site-specific integration in planta -- Targeted mutagenesis in arabidopsis using zinc-finger nucleases -- Vectors and methods for hairpin RNA and artificial microRNA-mediated gene silencing in plants -- Recombinant protein expression in nicotiana -- Chromosome analysis and sorting using flow cytometry -- Super-stretched pachytene chromosomes for plant molecular cytogenetic mapping -- Cytological dissection of the triticeae chromosomes by the gametocidal system -- Development and use of oat-maize chromosome additions and radiation hybrids -- Enhancer trapping in plants -- Chromatin beacons : Global sampling of chromatin physical properties using chromatin charting lines -- Transposable elements as catalysts for chromosome rearrangements.
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Plant functional genomics Springer Protocols
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Plant genomes 2008, KargerPaleopolyploidy and its impact on the structure and function of modern plant genomes / A.H. Paterson -- Genomic history and gene family evolution in angiosperms: challenges and opportunities / J. Sampedro, D. Cosgrove -- The evolutionary position of subfunctionalization, downgraded / M. Freeling -- Grass genome structure and evolution / J. Messing, J.L. Bennetzen -- Phylogenetic insights into the pace and pattern of plant genome size evolution / C.E. Grover, J.S. Hawkins, J.F. Wendel -- Plant transposable elements / J.M. Deragon, J.M. Casacuberta, O. Panaud -- Plant sex chromosomes / D. Charlesworth -- Plant centromeres / J.C. Lamb ... [et al.] -- MiRNAs in the plant genome: all things great and small / B.C. Meyers, P.J. Green, C. Lu -- Recent insights into the evolution of genetic diversity of maize / A. Rafalski, S. Tingey -- The rice genome structure as a trail from the past to beyond / T. Sasaki.
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Plant genomics 2009, Springer ProtocolsRole of model plant species / Richard Flavell -- New technologies for ultra-high throughput genotyping in plants / Nikki Appleby, David Edwards, and Jacqueline Batley -- Genetic maps and the use of synteny / Chris Duran, David Edwards, and Jacqueline Batley -- A simple TAE-based method to generate large insert BAC libraries from plant species / Bu-Jun Shi, J. Perry Gustafson, and Peter Langridge -- Transcript profiling and expression level mapping / Elena Potokina, Arnis Druka, and Michael J. Kearsey -- Methods for functional proteomic analyses / Christof Rampitsch and Natalia V. Bykova -- Stable transformation of plants / Huw D. Jones and Caroline A. Sparks -- Transient transformation of plants / Huw D. Jones, Angela Doherty, and Caroline A. Sparks -- Bridging the gene-to-function knowledge gap through functional genomics / Stephen J. Robinson and Isobel A.P. Parkin -- Heterologous and cell-free protein expression systems / Naser Farrokhi ... [et al.] -- Functional genomics and structural biology in the definition of gene function / Maria Hrmova and Geoffrey B. Fincher -- In situ analysis of gene expression in plants / Sinéad Drea ... [et al.] -- Plant and crop databases / David E. Matthews, Gerard R. Lazo, and Olin D. Anderson -- Plant genome annotation methods / Shu Ouyang ... [et al.] -- Molecular plant breeding : methodology and achievements / Rajeev K. Varshney ... [et l.] -- Practical delivery of genes to the marketplace / David A. Fischhoff and Molly N. Cline -- Ecological genomics of natural plant populations : the Israeli perspective / Eviatar Nevo -- Genome sequencing approaches and successes / Michael Imelfort ... [et al.]
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Post-transcriptional gene regulation 2008, Springer ProtocolsBioinformatics approaches for studying untranslated regions of mRNAs / Paramjeet S. Bagga -- Identification of mRNA polyadenylation sites in genomes using cDNA sequences, expressed sequence tags, and trace / Ju Youn Lee, Ji Yeon Park, and Bin Tian -- Bioinformatic tools for studying post-transcriptional gene regulation : the UAlbany TUTR Collection and other informatic resources / Francis Doyle ... [et al.] -- In-line probing analysis of riboswitches / Elizabeth E. Regulski and Ronald R. Breaker -- Ribotrap : targeted purification of RNA-specific RNPs from cell lysates through immunoaffinity precipitation to identify regulatory proteins and RNAs / Dale L. Beach and Jack D. Keene -- Advances in RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray profiling / Timothy E. Baroni ... [et al.] -- Biosensors for RNA aptamers-protein interaction / Sara Tombelli, Maria Minunni, and Marco Mascini -- A tethering approach to study proteins that activate mRNA turnover in human cells / Sandra L. Clement and Jens Lykke-Andersen -- RNA analysis by biosynthetic tagging using a 4-thiouracil and uracil phosphoribosyltransferase / Gusti M. Zeiner ... [et al.] -- Efficient 5' cap-dependent RNA purification : use in identifying and studying subsets of RNA / Edyta Z. Bajak and Curt H. Hagedorn -- Enrichment of alternatively spliced isoforms / Julian P. Venables --In vivo methods to assess polyadenylation efficiency / Lisa K. Hague, Tyra Hall-Pogar, and Carol S. Lutz -- Monitoring the temporal and spatial distribution of RNA in living yeast cells / Roy M. Long and Carl R. Urbinati -- Analysis of mRNA partitioning between the cytosol and endoplasmic reticulum compartments of mammalian cells / Samuel B. Stephens ... [et al.] -- In vivo and in vitro analysis of poly(A) length effects on mRNA translation / Jing Peng, Elizabeth L. Murray, and Daniel R. Schoenberg -- A ribosomal density-mapping procedure to explore ribosome positions along translating mRNAs / Naama Eldad and Yoav Arava -- Identification of changes in gene expression by quantitation of mRNA levels / Wendy M. Olivas -- Application of the Invader® RNA assay to the polarity of vertebrate mRNA decay / Elizabeth L. Murray and Daniel R. Schoenberg -- Development of an in vitro mRNA decay system in insect cells / Kevin Sokoloski, John R. Anderson, and Jeffrey Wilusz -- Using synthetic precursor and inhibitor miRNAs to understand miRNA function / Lance P. Ford and Angie Cheng -- A step-by-step procedure to analyze the efficacy of siRNA using real-time PCR / Angie Cheng, Charles L. Johnson, and Lance P. Ford.
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Principles of cancer genetics 2008, Springer
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Principles of clinical cytogenetics. 2nd ed. 2005, SpringerHistory of clinical cytogenetics / Steven L. Gersen -- DNA, chromosomes, and cell division / Martha Keagle -- Human chromosome nomenclature: an overview and definition of terms / Avirachan Tharapel -- Basic laboratory procedures / Martha B. Keagle and Steven L. Gersen -- Microscopy / Christopher McAleer -- Quality control and quality assurance / Michael Watson and Steven L. Gersen -- Instrumentation in the cytogenetics laboratory / Steven L. Gersen and Lotte Downey -- Autosomal aneuploidy / Jin-Chen C. Wang -- Structural chromosome rearrangements / Kathleen Kaiser-Rogers and Kathleen Rao -- Sex chromosomes and sex chromosome abnormalities / Cynthia M. Powell -- The cytogenetics of infertility / Linda Marie Randolph -- Prenatal cytogenetics / Linda Marie Randolph -- The cytogenetics of spontaneous abortion / Solveig M.V. Pflueger -- Chromosome instability / Xiao-Xiang Zhang -- Cytogenetics of hematologic neoplasms / Rizwan Naeem -- Cytogenetics of solid tumors / Jonathan Fletcher -- Genomic imprinting and uniparental disomy / Jin-Chen C. Wang -- Fluroescence in situ hybridization (FISH) / Daynna Wolff and Stuart Schwartz -- Fragile X: from cytogenetics to molecular genetics / Patricia Howard-Peebles and Dana Crawford -- Genomic imprinting and uniparental disomy / Jin-Chen C. Wang -- Genetic counseling / Sarah Hutchings Clark.
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Principles of cloning 2002, ScienceDirectHistorical perspective / Robert H. Foote -- Activation of mammalian oocytes / Rafael A. Fissore, Jeremy Smyth, Manabu Kurokawa and Philippe Collas -- The nucleus / Fyodor D. Urnov and Alan P. Wolffe -- Nuclear reprogramming : biological and technological constraints / Kevin Eggan and Rudolf Jaenisch -- Plasticity of somatic nucleus by epigenetic reprogramming via cell hybridization / Takashi Tada, Masako Tada and M. Azim Surani -- Determinants of pluripotency in mammals / Michele Boiani and Hans R. Schöler -- Micromanipulation techniques for cloning / Raymond L. Page -- Microinsemination and nuclear transfer with male germ cells / Atsuo Ogura, Narumi Ogonuki and Kimiko Inoue -- Development of viable mammalian embryos in vitro : evolution of sequential media / David K. Gardner and Michelle Lane -- Genetic and phenotypic similarity among members of mammalian clonal sets / George E. Seidel, Jr. -- Genetic modification and cloning in mammals / Patrick W. Dunne and Jorge A. Piedrahita -- Pregnancy and neonatal care of cloned animals / Jonathan R. Hill and Pascale Chavatte-Palmer -- Donor cell type and cloning efficiency in mammals / Y. Tsunoda and Y. Kato -- Cloning of amphibians / J.B. Gurdon and J.A. Byrne -- Cloning of fish / Yuko Wakamatsu and Kenjiro Ozato -- Cloning of mice / Teruhiko Wakayama and Anthony C.F. Perry -- Cloning of rabbits / András Dinnyés, X. Cindy Tian and Xiangzhong Yang -- Nuclear transfer in swine / Randall S. Prather -- Cloning of cattle / Neal L. First, Zeki Beyhan and Jennifer D. Ambroggio -- Cell cycle regulation in cloning / Keith H.S. Campbell -- Cloning of rats / Philip Iannaccone, Michael Bader and Vasiliy Galat -- Cloning in nonhuman primates / Tanja Dominko, Calvin Simerly, Crista Martinovich and Gerald Schatten -- Nuclear transfer for stem cells / Alan Trounson -- Current research and commercial applications of cloning technology / Steven L. Stice -- Transgenic cloned goats and the production of therapeutic proteins / Esmail Behboodi, LiHow Chen, Margaret M. Destrempes, Harry M. Meade and Yann Echelard -- Ethical implications of cloning / Ronald M. Green -- Mammalian cloning : challenges for the future / R.L. Gardner.
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Principles of molecular medicine. 2nd ed. 2006, Springer
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PRINS and in situ PCR protocols. 2nd ed. Springer Protocols
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Protein engineering ScienceDirect
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Protein engineering 2009, SpringerUnderstanding enzyme mechanism through protein chimeragenesis / N.M. Goodey and S.J. Benkovic -- Chemical protein engineering : synthetic and semisynthetic peptides and proteins / L. Merkel, L. Moroder, and N. Budisa -- Native chemical ligation: Semisynthesis of post-translationally modified proteins and biological probes / E. Vogel Taylor and B. Imperiali -- Chemical methods for mimicking post-translational modifications / S.I. van Kasteren, P. Garnier and B.G. Davis -- Noncanonical amino acids in protein science and engineering / K.E. Beatty and D.A. Tirrell -- Fidelity mechanisms of the aminoacyl-tRNA synthetases / A.P. Mascarenhas ... [et al.] -- Specialized components of the translational machinery for unnatural amino acid mutagenesis : tRNAs, aminoacyl-tRNA synthetases, and ribosomes / C. Köhrer and U.L. RajBhandary -- In vivo studies of receptors and ion channels with unnatural amino acids / D.A. Dougherty -- Synthesis of modified proteins using misacylated tRNAs / S.M. Hecht -- Cell-free synthesis of proteins with unnatural amino acids. The pure system and expansion of the genetic code / I. Hirao, T. Kanamori and T. Ueda -- Engineering nucleobases and polymerases for an expanded genetic alphabet / A.M. Leconte and F.E. Romesberg -- Understanding membrane proteins. How to design inhibitors of transmembrane protein-protein interactions / J.S. Slusky, H. Yin and W.F. DeGrado.
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Protein engineering protocols Springer Protocols
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Protocols for nucleic acid analysis by nonradioactive probes. 2nd ed. Springer ProtocolsGenomic DNA isolation from different biological materials / Duckchul Park -- Extraction of plant RNA / Elspeth MacRae -- Overview on hybridization and detection techniques / Elena Hilario -- Checkerboard DNA/DNA hybridization technology : using DIG detection / Lisa S. Gellen, Glenn Wall-Manning, and Chris H. Sissons -- Nonradioactive northern and southern analyses from plant samples / Christoph Peterhaensel, Dagmar Weier, and Thomas Lahaye -- Screening a BAC library with nonradioactive overlapping oligonucleotide (overgo) probes / Elena Hilario, Tiffany F. Bennell, and Erik Rikkerink -- Direct in-gel hybridization of DNA with digoxigenin-labeled probes / Saeed A. Khan and Mohamed S. Nawaz --In situ hybridization of termite microbes / Shigeharu Moriya ... [et al.] -- RNA electrophoretic mobility shift assay using a fluorescent DNA sequencer / Yukinori Eguchi -- Comparative quantitation of mRNA expression in the central nervous system using fluorescence in situ hybridization / Darren J. Day, Eli M. Mrkusich, and John H. Miller -- Visualization of gene expression by fluorescent multiplex real-time PCR amplification / Maria Rosa Ponce ... [et al.] -- Fluorescence in situ hybridization for the identification of environmental microbes / Annelie Pernthaler and Jakob Pernthaler -- Introduction to kinetic (real-time) PCR / John Mackay -- Validation of siRNA knockdowns by quantitative real-time PCR / Sukru Tuzmen --Real-time quantitative PCR as an alternative to southern blot or fluorescence in situ hybridization for detection -- Of gene-copy number changes / Jasmien Hoebeeck, Frank Speleman, and Jo Vandesompele -- Design and work-up of a new molecular diagnostic assay based on real-time PCR / Harald Kessler -- Real-time PCR fluorescent chemistries / John Mackay and Olfert Landt -- Microarrays : an overview / Norman H. Lee and Alexander I. Saeed -- Oligonucleotide microarrays for the study of coastal microbial communities / Gaspar Taroncher-Oldenburg and Bess B. Ward.
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Psychiatric genetics Springer Protocols
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Pyrosequencing protocols Springer ProtocolsThe history of pyrosequencing / Pol Nyron -- Pyrosequencing applications / Sharon Marsh -- Web-based primer design software for genome-scale genotyping by pyrosequencing / Steven Ringquist ... [et al.] -- Pyrosequencing : a simple method for accurate genotyping / Cristi R. King and Tiffany Scott-Horton -- Universal primer applications for pyrosequencing / Dong-Chuan Guo and Dianna M. Milewicz -- Allele quantification and DNA pooling methods / Jonathon Wasson -- Multiplex pyrosequencing for DNA variation analysis / Pritesh Patel, Yih-Horng Shiao, and Paolo Fortina -- Analysis of gene-specific DNA methylation patterns by the pyrosequencing technology / Jørg Tost and Ivo Glynne Gut -- Detecting mutations that confer oxazolidinone resistance in gram-positive bacteria / Neil Woodford, Sarah E. North, and Matthew J. Ellington -- Pyrosequencing-based strategies for improved allele typing of HLA loci / Steven Ringquist ... [et al.] -- Pyrosequencing of phage display libraries for the identification of cell-specific targeting ligands / Ahad A. Rahim -- Gene copy number detection in animal studies / Gerli Pielberg and Leif Andersson -- Detection of allelic imbalance in gene expression using pyrosequencing / Hua Wang and Steven C. Elbein -- Pyrosequencing genotype storage techniques / Derek J. Van Booven.
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Quantitative trait loci Springer Protocols
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Recoding 2010, SpringerPart I. Redefinition -- 1. Selenocysteine Biosynthesis, Selenoproteins, and Selenoproteomes / Vadim N. Gladyshev and Dolph L. Hatfield -- 2. Reprogramming the Ribosome for Selenoprotein Expression: RNA Elements and Protein Factors / Marla J. Berry and Michael T. Howard -- 3. Translation of UAG as Pyrrolysine / Joseph A. Krzycki -- 4. Specification of Standard Amino Acids by Stop Codons / Olivier Namy and Jean-Pierre Rousset -- 5. Ribosome “Skipping”: “Stop-Carry On” or “StopGo” Translation / Jeremy D. Brown and Martin D. Ryan -- 6. Recoding Therapies for Genetic Diseases / Kim M. Keeling and David M. Bedwell -- Part II. Frameshifting - Redirection of Linear Readout -- 7. Pseudoknot-Dependent Programmed -1 Ribosomal Frameshifting: Structures, Mechanisms and Models / Ian Brierley, Robert J.C. Gilbert, and Simon Pennell -- 8. Programmed -1 Ribosomal Frameshift in the Human Immunodeficiency Virus of Type 1 / Léa Brakier-Gingras and Dominic Dulude -- 9. Ribosomal Frameshifting in Decoding Plant Viral RNAs / W. Allen Miller and David P. Giedroc -- 10. Programmed Frameshifting in Budding Yeast / Philip J. Farabaugh -- 11. Recoding in Bacteriophages / Roger W. Hendrix -- 12. Programmed Ribosomal -1 Frameshifting as a Tradition: The Bacterial Transposable Elements of the IS3 Family / Olivier Fayet and Marie-Françoise Prère -- 13. Autoregulatory Frameshifting in Antizyme Gene Expression Governs Polyamine Levels from Yeast to Mammals / Ivaylo P. Ivanov and Senya Matsufuji -- 14. Sequences Promoting Recoding Are Singular Genomic Elements / Pavel V. Baranov and Olga Gurvich -- 15. Mutants That Affect Recoding / Jonathan D. Dinman and Michael O'Connor -- 16. The E Site and Its Importance for Improving Accuracy and Preventing Frameshifts / Markus Pech, Oliver Vesper, Hiroshi Yamamoto, Daniel N. Wilson, and Knud H. Nierhaus -- Part III. Discontiguity -- 17. Translational Bypassing - Peptidyl-tRNA Re-pairing at Non-overlapping Sites / Norma M. Wills -- 18. trans-Translation / Kenneth C. Keiler and Dennis M. Lee -- Part IV. Transcription Slippage -- 19. Transcript Slippage and Recoding / Michael Anikin, Vadim Molodtsov, Dmitry Temiakov, and William T. McAllister -- Part V. Appendix -- 20. Computational Resources for Studying Recoding / Andrew E. Firth, Michaël Bekaert, and Pavel V. Baranov.
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Recombinant gene expression. 2nd ed. Springer ProtocolsCopy-control tightly regulated expression vectors based on pBAC/oriV / Jadwiga Wild and Waclaw Szybalski -- Cell-free protein synthesis with prokaryotic combined transcription-translation / James R. Swartz, Michael C. Jewett, and Kim A. Woodrow -- Genetic tools for the manipulation of moderately halophilic bacteria of the family Halomonadaceae / Carmen Vargas and Joaquín J. Nieto -- Gene transfer and expression of Recombinant proteins in moderately halophilic bacteria / Amalia S. Afendra ... [et al.] -- Recombinant protein production in antarctic gram-negative bacteria / Angela Duilio, Maria Luisa Tutino, and Gennaro Marino -- Recombinant protein production in yeasts / Danilo Porro and Diethard Mattanovich -- Controlled expression of homologous genes by genomic promoter replacement in the yeast Saccharomyces cerevisiae / Kevin J. Vestrepen and Johan M. Thevelein -- High-throughput expression in microplate format in Saccharomyces cerevisiae / Caterina Holz and Christine Lang -- High-throughput expression in microplate format in Pichia pastoris / Mewes Böttner and Christine Lang -- Multiple gene expression by chromosomal integration and CRE-loxP-mediated marker recycling in Saccharomyces cerevisiae / Björn Johansson and Bärbel Hahn-Hägerdal --Flp-mediated integration of expression cassettes into FRT-tagged chromosomal loci in mammalian cells / Dagmar Wirth and Hansjörg Hauser -- Generation of high-recombinant protein-producing Chinese hamster ovary (CHO) cells / Jean-Louis Goergen and Lucía Monaco -- Preparation of recombinant proteins in milk / Louis-Marie Houdebine.Host cell compatibility in protein expression / James J. Greene -- Production of Recombinant proteins: challenges and solutions / Laura A. Palomares, Sandino Estrada-Moncada and Octavio T. Ramírez -- Folding promoting agents in Recombinant protein production / Beatrix Fahnert -- Back to basics: pBR322 and protein expression systems in e. coli / Paulina Balbás and Francisco Bolívar -- alpha-complementation enabled T7 expression vectors and their use for the expression of recombinant polypeptides for protein transduction experiments / Mikhail F. Alexeyev ... [et al.] -- Expression of recombinant alkaline phosphatase conjugates in Escherichia coli / Jean-Claude Boulain and Frédéric Ducancel -- Overexpression of chromosomal genes in Escherichia coli / Fernando Valle and Noemí Flores -- Chromosomal expression of foreign and native genes from regulatable promoters in Escherichia coli / Lu Zhou, Ke Zhang, and Barry L. Wanner -- Plasmid vectors for marker-free chromosomal insertion of genetic material in Escherichai coli / Sylvie Le Borgne, Francisco Bolívar, and Guillermo Gosset -- Copy-control pBAC/oriV vectors for genomic cloning / Jadwiga Wild and Waclaw Szybalski --Three decades of fungal transformation: key concepts and applications / Vianey Olmedo-Monfil, Carlos Cortés-Penagos, and Alfredo Herrera-Estrella -- Three decades of fungal transformation: novel technologies / Sergio Casas-Flores, Teresa Rosales-Saavedra, and Alfredo Herrera-Estrella -- Gene transfer and expression in plants / Argelia Lorence and Robert Verpoorte -- Production of Recombinant proteins by hairy roots cultured in plastic sleeve bioreactors / Fabricio Medina-Bolívar and Carole Cramer -- Engineering the chloroplasts genome for hyperexpression of human therapeutic proteins and vaccine antigens / Shashi Kumar and Henry Daniell -- New selection marker for plant transformation / Barbara Leyman ... [et al.] -- Enhancer detection and gene trapping as tools for functional genomics in plants / Gerardo Acosta-García, Daphné Autran, and Jean-Philippe Vielle-Calzada -- Gene transfer and expression in mammalian cell lines and transgenic animals / Félix Recillas-Targa -- Sustained heterologous transgene expression in mammalian and avian cell lines / Héctor Rincón-Arano and Félix Recillas-Targa -- Inducible gene expression in mammalian cells and mice / Wilfried Webber and Martin Fussenegger --
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Redox cell biology and genetics. Part A-B ScienceDirectFulltext (pt. B) ScienceDirect
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Regenerative biology and medicine 2006, ScienceDirect
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Regulation of gene expression in plants 2007, Springer
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Regulation of microRNAs 2011, SpringerMicroRNA biogenesis and function : an overview / Rene F. Ketting -- Regulation of pri-miRNA processing through Smads / Akiko Hata and Brandi N. Davis -- Stimulation of pri-miR-18a processing by hnRNP A1 / Gracjan Michlewski, Sonia Guil, and Javier F. Cáceres -- KSRP promotes the maturation of a group of miRNA precursors / Michele Trabucchi ... [et al.] -- Hormonal repression of miRNA biosynthesis through a nuclear steroid hormone receptor / Sally Fujiyama-Nakamura, Kaoru Yamagata, and Shigeaki Kato -- Autoregulatory mechanisms controlling the microprocessor / Robinson Triboulet and Richard I. Gregory -- Regulation of pre-miRNA processing / Nicolas J. Lehrbach and Eric A. Miska -- The effect of RNA editing and ADARs on miRNA biogenesis and function / Bret S.E. Heale, Liam P. Keegan, and Mary A. O'Connell -- MiRNAs need a trim regulation of miRNA activity by trim-NHL proteins / F. Gregory Wulczyn ... [et al.] -- Properties of the regulatory RNA-binding protein HuR and its role in controlling miRNA repression / Nicole-Claudia Meisner and Witold Filipowicz -- Turnover of mature miRNAs and siRNAs in plants and algae / Heriberto Cerutti and Fadia Ibrahim -- MicroRNases and the regulated degradation of mature animal miRNAs / Helge Grosshans and Saibal Chatterjee.
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Regulation of phytochemicals by molecular techniques. 1st ed. 2001, ScienceDirect
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Regulatory genome 2006, ScienceDirect
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Regulatory RNAs Cold Spring Harb Lab PressMechanism and biology of RNAi -- Genome-wide approaches -- Small RNAs in development -- Telomeres and cancer -- End regulation of transcripts -- RNPs and RNA editing -- Biology of short RNAs -- Control of gene expression by noncoding RNAs -- Heterochromatin -- Quality control, messenger RNA turnover, and translational control.
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Regulatory RNAs 2012, Springerpt. 1. Basics -- pt. 2. Methods -- pt. 3. Applications.
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Reporter genes 2007, Springer ProtocolsMethodologies for staining and visualization of [beta]-galactosidase in mouse embryos and tissues / Siobhan Loughna and Deborah Henderson -- Immunohistochemical detection of [beta]-galactosidase or green fluorescent protein on tissue sections / Philip A. Seymour and Maike Sander -- Detection of reporter gene expression in murine airways / Maria Limberis, Peter Bell, and James M. Wilson -- Three-dimensional analysis of molecular signals with episcopic imaging techniques / Wolfgang J. Weninger and Timothy J. Mohun -- Fluorescent proteins for cell biology / George H. Patterson -- Detection of GFP during nervous system development in Drosophila melanogaster / Karin Edoff, James S. Dods, and Andrea H. Brand -- Autofluorescent proteins for flow cytometry / Charles G. Bailey and John E. J. Rasko -- Fluorescent protein reporter systems for single-cell measurements / Steven K. Dower, Eva E. Qwarnstrom, and Endre Kiss-Toth -- Subcellular imaging of cancer cells in live mice / Robert M. Hoffman -- Noninvasive imaging of molecular events with bioluminescent reporter genes in living subjects / Pritha Ray and Sanjiv Sam Gambhir -- Green fluorescent protein as a tracer in chimeric tissues : the power of vapor fixation / Harald Jockusch and Daniel Eberhard.
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Research in computational molecular biology Springer, 2007
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Research in computational molecular biology Springer, 2005
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Retrotransposition, diversity, and the brain 2008, Springer
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Reviews and protocols in DT40 research 2006, SpringerCh. 1. DT40 gene disruptions: a how-to for the design and the construction of targeting vectors / Hiroshi Arakawa and Jean-Marie Buerstedde -- Ch. 2. Immunoglobulin gene conversion or hypermutation: That's the question / Jean-Marie Buerstedde and Hiroshi Arakawa -- Ch. 3. Genome resources for the DT40 community / Randolph B Caldwell and Andrzej M. Kierzek -- Ch. 4. Chromosome engineering in DT40 cells and mammalian centromere function / William R.A. Brown ... [et al.] -- Ch. 5. Function of recQ family helicase in genome stability / Masayuki Seki, Shusuke Tada and Takemi Enomoto -- Ch. 6. Genetic analysis of apoptotic execution / Sandrine Ruchaud ... [et al.] -- Ch. 7. The DT40 system as a tool for analyzing kinetochore assembly / Masahiro Okada, Tesuya Hori and Tatsuo Fukagawa -- Ch. 8. Analysing the DNA damage and replication checkpoints in DT40 cells / Michael D Rainey, George Zachos, and David AF Gillespie -- Ch. 9. Using DT40 to study clathrin function / Frank R. Wettey and Antony P. Jackson -- Ch. 10. Genetic analysis OF B cell signaling / Hisaaki Shinohara and Tomohiro Kurosaki --Ch. 11. DT40 mutants: a model to study transcriptional regulation of B cell development and function / Jukka Alinikula, Olli Lassila and Kalle-Pekka Nera -- Ch. 12. Transcription and RNA processing factors play complex roles IN DT40 cells / Stephanie Bush and James L. Manley -- Ch. 13. Participation of histones, histone modifying enzymes and histone chaperones in vertebrate cell functions / Hidehiko Kikuchi ... [et al.] -- Ch. 14. Analysis of gene expression, copy number and palindrome formation with a DT40 enriched cDNA Microarray / Paul E. Neima ... [et al.] -- Ch. 15. Analysis of DNA replication damage bypass and its role in immunoglobulin repertoire development / Julian E. Sale, Anna-Laura Ross and Laura J. Simpson -- Ch. 16. The fanconi anemia pathway promotes homologous recombination repair in DT40 cell line / Julian E. Sale, Anna-Laura Ross and Laura J. Simpson -- Ch. 17. Calcium signaling, ion channels and more / Anne-Laure Perraud, Carsten Schmitz, Andrew M. Scharenberg -- Ch. 18. Phenotypic analysis of cellular responses to DNA damage / Helfrid Hochegger and Shunichu Takeda -- Ch. 19. ATM, a paradigm for a stress-responsive signal transducer in higher vertebrate cells / Ken-ichi Yamamoto, Masahiko Kobayashi and Hiroko Shimizu --METHOD 1. Stable transfection of DT40 / Julian E. Sale -- METHOD 2. Basic cell culture conditions / Huseyin Saribasak and Hiroshi Arakawa -- METHOD 3. Excision of floxed-DNA sequences by transient induction of Mer-Cre-Mer / Hiroshi Arakawa -- METHOD 4. Immunoglobulin gene conversion and hypermutation assay by FACS / Hiroshi Arakawa -- METHOD 5. Target screening by PCR / Hiroshi Arakawa -- METHOD 6. Mitotic index determination by flow cytometry / David AF Gillespie and Mark Walker -- METHOD 7. Centrifugal elutriation as a means of cell cycle phase separation and synchronization / David AF Gillespie and Catarina Henriques -- METHOD 8. Preparation of genomic DNA for microarray-based comparative genome hybridization / Robert Kimmel, Amalia Icreverzi, Paul Neiman -- METHOD 9. Analysis of cellular Mg2+ in DT40 cells / Anne-Laure Perraud, Carsten Schmitz, Andrew M. Scharenberg -- METHOD 10. Transient transfection of DT40 / Roger Franklin & Julian E. Sale -- METHOD 11. Retroviral transduction of DT40 / Felix Randow & Julian E. Sale -- METHOD 12. Colony survival assay / Laura. J. Simpson & Julian E. Sale -- METHOD 13. Subcloning DT40 by limiting dilution / Jean-Marie Buerstedde --METHOD 14. Subnuclear immunofluorescence / Dávid Szüts & Julian E. Sale -- METHOD 15. Sister chromatid exchange assay / Laura J. Simpson & Julian E. Sale -- METHOD 16. 2D cell cycle analysis / Roger Franklin & Julian E. Sale -- METHOD 17. Purification of tap-tagged proteins by two-step pull down from DT40 cells / Hiroyuki Kitao and Minoru Takata -- METHOD 18. Synchronization of cells / Eiichiro Sonoda -- METHOD 19. Tranfection of DT40 cells / Huseyin Saribasak and Hiroshi Arakawa -- METHOD 20. Luciferase reporter assay / Frank R. Wettey and Antony P. Jackson -- METHOD 21. Indirect immunofluorescence microscopy / Frank R. Wettey and Antony P. Jackson -- METHOD 22. Quantification of receptor-mediated endocytosis / Frank R. Wettey and Antony P. Jackson -- METHOD 23. Measurement of DNA synthesis and strand breaks using alkaline sucrose density gradient centrifugation / Kouichi Yamada and Jun Takezawa -- METHOD 24. Fractionation of nuclear and cytoplasmic proteins from DT40 cell lines / Yan-Dong Wang and Randolph B Caldwell.
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Ribozymes and siRNA protocols. 2nd ed. Springer Protocols
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RNA 2001, ScienceDirect
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RNA 2008, Springer ProtocolsThe transcriptional landscape -- Working with RNA -- Synthesis of RNA by in vitro transcription -- Efficient poly(A)+ RNA selection using LNA oligo(T) capture -- Genome browsers -- Web-based tools for studying RNA structure and function -- Northern blotting analysis -- Rapid amplification of cDNA ends (RACE) -- Fractionation of mRNA based on the length of the poly(A) tail -- Analysis of mutations that influence pre-mRNA splicing -- S1 nuclease analysis of alternatively spliced mRNA -- Promises and challenges in developing RNAi as a research tool and therapy -- Inhibition of gene function in mammalian cells using short-hairpin RNA (shRNA) -- Validation of RNAi by real time PCR -- Profiling RNA polymerase II using the fast chromatin immunoprecipitation method -- The post-transcriptional operon -- RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray (chip) profiling -- Isolation of RNP granules -- Electrophoretic mobility shift assay for characterizing RNA-protein interaction -- Polysome analysis and RNA purification from sucrose gradients -- Prediction of targets for microRNAs -- Outsourcing of experimental work.
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RNA editing ScienceDirect
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RNA editing 2008, SpringerEditing reactions from the perspective of RNA structure / Matthias Homann -- Editing of tRNA for structure and function / Juan D. Alfonzo -- RNA editing by adenosine deaminases that act on RNA (ADARs) / Michael F. Jantsch and Marie Öhman -- Insertion/deletion editing in Physarum polycephalum / Jonatha M. Gott and Amy C. Rhee -- RNA editing in plant mitochondria / Mizuki Takenaka ... [et al.] -- RNA editing in chloroplasts / Masahiro Sugiura -- Working together : the RNA editing machinery in Trypanosoma brucei / Jason Carnes and Kenneth Stuart -- RNA editing accessory factors : the example of mHel61p / H. Ulrich Göringer ... [et al.] -- The function of RNA editing in trypanosomes / Torsten Ochsenreiter and Stephen Hajduk -- Evolutionary aspects of RNA editing / Dave Speijer.
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RNA exosome 2011, Springer"The diversity of RNAs inside living cells is amazing. We have known of the more "classic" RNA species: mRNA, tRNA, rRNA, snRNA and snoRNA for some time now, but in a steady stream new types of molecules are being described as it is becoming clear that most of the genomic information of cells ends up in RNA. To deal with the enormous load of resulting RNA processing and degradation reactions, cells need adequate and efficient molecular machines. The RNA exosome is arising as a major facilitator to this effect. Structural and functional data gathered over the last decade have illustrated the biochemical importance of this multimeric complex and its many co-factors, revealing its enormous regulatory power. By gathering some of the most prominent researchers in the exosome field, it is the aim of the book to introduce this fascinating protein complex and to give a timely and rich account of its many functions"--Provided by publisher.
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RNA infrastructure and networks 2011, SpringerThe RNA infrastructure : an introduction to ncRNA networks / Lesley J. Collins -- RNA interactions / Manja Marz and Peter F. Stadler -- Plant RNA silencing in viral defence / Vitantonio Pantaleo -- Micrornas as post-transcriptional machines and their interplay with cellular networks / Sarath Chandra Janga and Swathi Vallabhaneni -- Spatiotemporal aspects of microRNA-mediated gene regulation / Joris Pothof and Dik C. van Gent -- Spliceosomal RNA infrastructure : the network of splicing components and their regulation by miRNAs / Lesley J. Collins -- Construction, structure, and dynamics of post-transcriptional regulatory network directed by RNA binding proteins / Sarath Chandra Janga and Nitish Mittal -- Safe keeping the message : mRNP complexes tweaking after transcription / Said Hafidh, Věra Čapková, and David Honys -- Unexpected functions of tRNA and tRNA processing enzymes / Rebecca L. Hurto -- Programmed DNA elimination in tetrahymena : a small RNA mediated genome surveillance mechanism / Kensuke Kataoka and Kazufumi Mochizuki -- Long noncoding RNA and epigenomics / Chandrasekhar Kanduri -- Promoter associated long noncoding RNAs repress transcription through a RNA binding protein TLS / Riki Kurokawa -- RNA networks in prokaryotes I :CRISPRs and riboswitches / Patrick J. Biggs and Lesley J. Collins -- RNA networks in prokaryotes II : tRNA processing and small RNAs / Lesley J. Collins and Patrick J. Biggs -- Localization of the bacterial RNA infrastructure / Kenneth C. Keiler -- Small RNA discovery and characterisation in eukaryotes using high throughput approaches / Helio Pais ... [et al.] -- How old are RNA networks? / Toni Daly, X. Sylvia Chen, and David Penny.
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RNA interference ScienceDirect
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RNA interference technology 2005, MyiLibraryChemical modifications to achieve increased stability and sensitive detection of siRNA -- RNA interference in postimplantation mouse embryos -- In ovo RNAi opens new possibilities for functional genomics in vertebrates -- Sect. 4. Gene silencing in model organisms. Practical applications of RNAi in C. elegans -- Inducible RNAi as a forward genetic tool in Trypanosoma brucei -- RNA-mediated gene silencing in fission yeast -- RNA silencing in filamentous fungi: Mucor ciccinelloides as a model organism -- RNAi and gene silencing phenomena mediated by viral suppressors in plants --Sect. 1. Basic RNAi, siRNA, microRNAs and gene-silencing mechanisms. RNAi beginnings. Overview of the pathway in C. elegans -- Dicer in RNAi: its roles in vivo and utility in vitro -- Genes required for RNA interference -- MicroRNAs: a small contribution from worms -- miRNAs in the brain and the application of RNAi to neurons -- Sect. 2. Design, synthesis of siRNAs. Degign and synthesis of small interfering RNA (siRNA) -- Automated design and high throughput chemical synthesis of siRNA -- Rational design of siRNAs with the Sfold software -- Enzymatic production of small interfering RNAs -- Sect. 3. Vector development and in vivo, in vitro and in ovo delivery methods. Six methods of inducing RNAi in mammalian cells -- Viral delivery of shRNA -- siRNA delivery by lentiviral vectors: design and applications -- Liposomal delivery of siRNAs in mice --Sect. 5. Drug target validation. Delivering siRNA in vivo for functional genomics and novel therapeutics -- The role of RNA interference in drug target validation: application to Hepatitis C -- RNAi in the drug discovery process -- RNA interference technology in the discovery and validation of druggable targets -- Sect. 6. Therapeutic and drug development. RNAi-mediated silencing of viral gene expression and replication -- RNAi in drug development: practical considerations -- RNA interference studies in liver failure -- RNAi applications in living animal systems -- Sect. 7. High-throughput genome-wide RNAi analysis. High-throughput RNAi by soaking in Caenorhabtis elegans -- Tools for integrative genomics: genome-wide RNAi and expression profiling in Drosophila -- Microarray analysis and RNA silencing to determine genes functionally important in mesothelioma -- High-throughput RNA interference -- Generation of highly specific vector-based shRNAi libraries directed against the entire human genome.
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RNA interference, editing, and modification Springer Protocols
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RNA methodologies. 4th ed. 2010, ScienceDirectRNA and the cellular biochemistry revisited -- RNA isolation strategies -- The truth about tissues -- Going green : RNA and the molecular biology of plants -- Isolation of polyadenylated RNA -- Quality control for RNA preparations -- Resilient ribonucleases -- Stringency : conditions that influence nucleic acid structure -- Electrophoresis of RNA -- Photodocumentation and image analysis -- Northern analysis -- Nucleic acid probe technology -- Practical nucleic acid hybridization -- Principles of detection -- Quantifications of specific mRNAs by nuclease protection -- Analysis of nuclear RNA -- cDNA synthesis -- RT-PCR : a science and an art form -- Quantitative PCR techniques -- Functional genomics and transcript profiling -- High-throughput analysis of gene expression -- Non-array methods for global analysis of gene expression -- RNAi : take a RISC-role the dicer -- Genomes, transcriptomes, proteomes, and bioinformatics -- An RNA paradigm.
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RNA modification ScienceDirect
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RNA polymerases and associated factors. Part C-D pt. C-D, 2003., ScienceDirectpt. D ScienceDirect
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RNA silencing Springer ProtocolsDetection of microRNAs and assays to monitor microRNA activities in vivo and in vitro / Marianthi Kiriakidou ... [et al.] -- High-throughput analysis of microRNA gene expression using sensitive probes / Mouldy Sioud and Øystein Røsok -- Modification of human U1 snRNA for inhibition of gene expression at the level of pre-mRNA / Peng Liu ... [et al.].Identification of components of RNAi pathways using the tandem affinity purification method / Mikiko C. Siomi and Haruhiko Siomi -- Separation of Drosophila RNA silencing complexes by native gel electrophoresis / John W. Pham and Erik J. Sontheimer -- Preparation and analysis of drosha / Yoontae Lee and V. Narry Kim -- RNA interference in Caenorhabditis elegans / Nathaniel R. Dudley and Bob Goldstein -- Down-regulating gene expression by RNA interference in Trypanosoma brucei / Christine E. Clayton ... [et al.] -- Analysis of double-stranded RNA and small RNAs involved in RNA-mediated transcriptional gene silencing / M. Florian Mette ... [et al.] -- Strategies for the design of random siRNA libraries and the selection of anti-GFP siRNAs / Mouldy Sioud and Sébastien Wälchli -- Silencing gene expression with dicer-generated siRNA pools / Jason W. Myers and James E. Ferrell Jr. --Inhibition of gene expression in vivo using multiplex siRNA / Sung-Suk Chae and Timothy Hla -- Gene silencing by a DNA vector based RNAi technology / Guangchao Sui and Yang Shi -- Plasmid-based RNA interference: construction of small-hairpin RNA expression vectors / Scott Q. Harper and Beverly L. Davidson -- SiRNA delivery in vivo / Mouldy Sioud -- Peptide-based strategy for siRNA delivery into mammalian cells / Federica Simeoni ... [et al.] -- Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells / Mingjie Li and John J. Rossi -- Exon-specific RNA interference: a tool to determine the functional relevance of proteins encoded by alternatively spliced mRNAs / Alicia M. Celotto, Joo-Won Lee, and Brenton R. Graveley -- Immunoprecipitation of microRNPs and directional cloning of microRNAs / Elisavet Maniataki, Maria Dels Angels De Planell Saguer, and Zissimos Mourelatos --
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RNA technologies and their applications 2010, SpringerThe key features of RNA silencing / Kuniaki Saito ... [et al.] -- Selected strategies for the delivery of siRNA in vitro and in vivo / Sandra D. Laufer ... [et al.] -- RNAi suppression and its application / Xiaoping Yi, Rui Lu -- Strategies to prevent siRNA-triggered cellular toxicity / Matthias Bauer -- RNAi in malignant brain tumors: relevance to molecular and translational research / Mitsutoshi Nakada ... [et al.] -- Silencing Huntington's disease gene with RNAi / Yu Zhang, Robert M. Friedlander -- Application of Dicer-substrate siRNA in pain research / Philippe Sarret ... [et al.] -- RNAi treatment of HIV-1 infection / Karin J. von Eije, Ben Berkhout -- Application of RNA interference to treat conditions associated with dysregulation of transient receptor potential vanilloid 1 channel / Vickram Ramkumar ... [et al.] -- Harnessing RNAi-based functional genomics to unravel the molecular complexity underlying skin pigment variation / Hsiang Ho ... [et al.] --mRNA structure and its effects on posttranscriptional gene silencing / Stephen I. Rudnick, Veenu Aishwarya, Alan M. Gewirtz -- Antisense RNA-mediated regulation of the p53 tumor suppressor / Marianne Farnebo, Klas G. Wiman -- Antisense oligonucleotides: insights from preclinical studies and clinical trials / Doreen Kunze, Kai Kraemer, Susanne Fuessel -- What can the new hammerhead ribozyme structures teach us about design? / William G. Scott -- microRNA biogenesis and its impact on RNA interference / Stefanie Grund, Sven Diederichs -- MicroRNAs in epithelial antimicrobial immunity / Jun Liu ... [et al.] -- Emerging roles of long noncoding RNAs in gene expression and intracellular organization / Tetsuro Hirose -- Noncoding RNAs as therapeutic targets / Maciej Szymański, Jan Barciszewski -- Noncoding RNAs at H19/IGF2 locus: role in imprinting, gene expression, and associated pathologies / Nahalie Berteaux, Nathalie Spruyt, Eric Adriaenssens.
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RNA turnover in bacteria, archaea and organelles 2008, ScienceDirect
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RNA turnover in eukaryotes 2008, ScienceDirect
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RNAi 2008, Springer Protocols
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RT-PCR protocols Springer Protocols
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Sequence - evolution - function 2003., NLM
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Sequence comparison 2009, Springer
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Serial analysis of gene expression (SAGE) 2008, Springer ProtocolsSAGE and LongSAGE / Annabeth Laursen Høgh and Kåre Lehmann Nielsen -- Robust-LongSAGE (RL-SAGE) : an improved LongSAGE method for high-throughput transcriptome analysis / Malali Gowda and Guo-Liang Wang -- aRNA LongSAGE : SAGE with antisense RNA / Anna M. Heidenblut -- SuperSAGE / Hideo Matsumura ... [et al.] -- Low-cost-medium throughput Sanger dideoxy sequencing / Kåre Lehmann Nielsen -- DeepSAGE : higher sensitivity and multiplexing of samples using a simpler experimental protocol / Kåre Lehmann Nielsen -- High-resolution, genome-wide mapping of chromatin modifications by GMAT / Tae-Young Roh and Keji Zhao -- 5'- and 3'-RACEfrom LongSAGE tags / Kåre Lehmann Nielsen -- Extraction and annotation of SAGE tags using sequence quality values / Jeppe Emmersen -- Correction of technology-related artifacts in serial analysis of gene expression / Viatcheslav R. Akmaev -- Duplicate ditag analysis in LongSAGE / Jeppe Emmersen -- Statistical comparison of two or more SAGE libraries : one tag at a time / Gerben J. Schaaf ... [et al.] -- Scaling of gene expression data allowing the comparison of different gene expression platforms / Fred van Ruissen ... [et al.] -- Clustering analysis of SAGE transcription profiles using a Poisson approach / Haiyan Huang, Li Cai, and Wing H. Wong -- Identifying nonspecific SAGE tags by context of gene expression / Xijin Ge and San Ming Wang.
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Single cell diagnostics Springer ProtocolsNoninvasive metabolic assessment of single cells / David K. Gardner -- Laser capture microdissection for analysis of single cells / Nobuki Nakamura ... [et al.] -- Fluorescence in situ hybridization (FISH) on single cells (sex determination and chromosome rearrangements) / Paul N. Scriven and Caroline Mackie Ogilvie -- Single cell PCR for PGD : methods, strategies, and limitations / Karen Sermon and Martine De Rycke -- Real-time quantitative PCR measurement of male fetal DNA in maternal plasma / Bernhard Zimmermann ... [et al.] -- Cell-free fetal DNA plasma extraction and real-time PCR quantification / Jill L. Maron, Kirby L. Johnson, and Diana W. Bianchi -- LATE-PCR and allied technologies : real-time detection strategies for rapid, reliable diagnosis from single cells / Kenneth E. Pierce and Lawrence J. Wangh -- Efficient isothermal amplification of the entire genome from single cells / Karen V. Schowalter, Jolene R. Fredrickson, and Alan R Thornhill -- Comparative genomic hybridization on single cells / Lucille Voullaire and Leeanda Wilton -- Generation of amplified RNAs and cDNA libraries from single mammalian cells / James Adjaye -- Real-time PCR to measure gene expression in single cells / Dagan Wells -- Gender determination and detection of aneuploidy in single cells using DNA array-based comparative genomic hybridization (array CGH) / Dong Gui Hui, Xin Yuan Guan, and Nicole Hussey -- Microarray technology for mutation analysis of low template DNA samples / Chelsea Salvado and David Cram.
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