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Cell Biology

  • Print Material
  • Also available: Print – 2010
    The adhesion GPCRs : gene repertoire, phylogeny, and evolution / Helgi B. Schith, Karl J.V. Nordstrom, and Robert Fredriksson -- Cadherins : developmental roles and future challenges / Caroline J. Formstone -- Latrophilin signalling in tissue polarity and morphogenesis / Tobias Langenhan and Andreas P. Russ -- GPS proteolytic cleavage of adhesion-GPCRs / Hsi-Hsien Lin ... [et al.] -- The latrophilins : "split-personality" receptors / John-Paul Silva and Yuri A. Ushkaryov -- Studies on the very large G protein-coupled-receptor : from initial discovery to determining its role in sensorineural deafness in higher animals / D. Randy McMillan and Perrin C. White -- Adhesion-GPCRs in the CNS / Natalie Strokes and Xianhua Piao -- GPR56 interacts with extracellular matrix and regulates cancer progression / Lei Xu -- Adhesion-GPCRs in tumorigenesis / Gabriela Aust -- Immunity and adhesion-GPCRs / Simon Yona, Hsi-Hsien Lin, and Martin Stacey -- CD97 in leukocyte trafficking / Jorg Hamann ... [et al.] -- The role of CD97 in regulating adaptive T-cell responses / Ian Spendlove and Ruhcha Sutavani -- F4/80 : the macrophage specific adhesion-GPCR and its role in immunoregulation / Hsi-Hsien Lin ... [et al.] -- Signal transduction mediated through adhesion-GPCRs / Norikazu Mizuno and Hiroshi Itoh -- Emerging roles of brain specific angiogenesis inhibitor 1 / Daeho Park and Kodi S. Ravichandran -- Adhesion-GPCRs in the male reproductive tract / Ben Davies and Christiane Kirchhoff.
  • Extracellular matrix -- Cytoskeleton -- Pseudopodia and adhesion structures -- Adhesive interactions of tissue cells with the extracellular matrix -- Cell migration -- Cell responses to chemical heterogeneity of substrata: adhesive islets or paths -- Topographic cell responses -- Intercellular adhesive interactions.
  • Cellular and Molecular Aspects of Adipose Tissue -- Isolation of Stem Cells from Human Adipose Tissue: Technique, Problems, and Pearls -- Principles of Liposuction -- Clinical Applications of Liposuction -- Principles of Autologous Fat Transplantation -- Clinical Applications of Autologous Fat Transplantation -- Adipose Stem Cells: From Liposuction to Adipose Tissue Engineering -- Storage of Adipose Stem Cells -- Engineered Scaffolds and Matrices: Tailored Biomaterials for Adipose Stem Cell Engineering -- Fundamentals and Principles of Biomolecules in Adipose Stem Cell Engineering -- Gene Therapy Used for Adipose Stem Cell Engineering -- Stem Cells from Human Adipose Tissue: A New Tool for Pharmacological Studies and for Clinical Applications -- Role of Adipose Stem Cells Therapy in Obesity -- Stem Cell Based Cardioregeneration and Adipose Tissue -- Adipose Stem Cell Engineering: Characterization and Current Application in Neurological Tissue Repair -- Adipose Stem Cell Engineering: Clinical Applications in Plastic and Reconstructive Surgery -- Orthopedic Use of Adipose-Derived Stem Cells -- Adipose-Derived Stem Cells: Characterization and Application in Urology -- Adipose Stem Cell Engineering: Characterization and Current Application in Otolaryngology -- Adipose Stem Cell Technologies for Tissue Regeneration in Dentistry -- Adipose Stem Cells, Tissue Engineering, and Solid Organ Transplantation and Regeneration -- Industrial Approaches to Adipose Stem Cells Engineering -- Future Research in Adipose Stem Cell Engineering -- Conclusions and Future Directions.
  • Preface: Obesity, Adipose Tissue and Cancer Progression -- Metabolic Perturbations Associated with Adipose Tissue Dysfunction and the Obesity-Cancer Link -- Increased Adiposity and Colorectal Cancer -- Adiposity and Diabetes in Breast and Prostate Cancer -- Increased Adipocity and Endometrial Cancer Risk -- Adipokines: Soluble Factors from Adipose Tissue Implicated in Cancer -- Animal Models to Study the Interplay between Cancer and Obesity -- Unraveling the local influence of tumor-surrounding adipose tissue on tumor progression: cellular and molecular actors involved -- Trafficking of Cells from Adipose Tissue to Tumor Microenvironment -- The Impact of Obesity Intervention on Cancer: Clinical Perspectives -- Index.
  • 1. Adipocyte Precursors: Developmental Origins, Self-Renewal, and Plasticity / Christian Dani and Nathalie Billon -- 2. Adipocyte Differentiation / José María Moreno-Navarrete and José Manuel Fernández-Real -- 3. Brown Adipose Tissue / Martin Klingenspor and Tobias Fromme -- 4. White Adipose Tissue / Stephane Gesta and C. Ronald Kahn -- 5. Sex Differences in Body Fat Distribution / Alain Veilleux and André Tchernof -- 6. Macrophages and Inflammation / Elise Dalmas, Joan Tordjman, Michèle Guerre-Millo, and Karine Clément -- 7. Adipocyte Growth and Factors Influencing Adipocyte Life Cycle / Srujana Rayalam and Clifton A. Baile -- 8. The Evolution of Mammalian Adipose Tissue / Caroline M. Pond -- 9. Dietary Determinants of Fat Mass and Body Composition / María A. Zulet, María J. Moreno-Aliaga, and J. Alfredo Martínez -- 10. The Genetic Determinants of Common Obesity-Susceptibility / Ruth J.F. Loos -- 11. Early Origins of Obesity and Developmental Regulation of Adiposity / Shalini Ojha and Helen Budge.
  • Adipose tissue protocols. 2nd ed. 2008
    Springer Protocols
  • Isolation and culture of rhesus adipose-derived stem cells -- Isolation of human adipose-derived stem cells from lipoaspirates -- Isolation of murine adipose-derived stem cells -- In vitro adult rat adipose tissue-derived stromal cell isolation and differentiation -- In vitro adult canine adipose tissue-derived stromal cell growth characteristics -- Adipogenic differentiation of adult equine mesenchymal stromal cells -- Isolation and culture of porcine adipose tissue-derived somatic stem cells -- Automated isolation and processing of adipose-derived stem and regenerative cells -- Methods for the purification and characterization of human adipose-derived stem cells -- Characterization of human adipose-derived stem cells using flow cytometry -- Evaluation of cellular and humoral immune responses to allogeneic adipose-derived stem/stromal cells -- Adipose-derived stromal cells: Cytokine expression and immune cell contaminants -- Gel-based and gel-free proteomic technologies -- Adipogenic differentiation of adipose-derived stem cells -- Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells -- Neural differentiation of human adipose tissue-derived stem cells -- Osteogenic differentiation strategies for adipose-derived mesenchymal stem cells -- Generation of adipose stromal cell-derived hepatic cells -- Adipose stem cell differentiation into smooth muscle cells -- Endothelial and cardiac regeneration from adipose tissues -- Epithelial differentiation of human adipose-derived stem cells -- Screening for epigenetic target genes that enhance reprogramming using lentiviral-delivered shRNA -- Adipogenic differentiation of human adipose-derived stem cells on 3D silk scaffolds -- A first approach for the production of human adipose tissue-derived stromal cells for therapeutic use -- Viral transduction of adipose-derived stem cells -- Use of adipose-derived stem cells in high-throughput screening to identify modulators of lipogenesis -- Preservation protocols for human adipose tissue-derived adult stem cells -- Soft tissue reconstruction -- Methods for analyzing microRNA expression and function during osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells -- The generation and the manipulation of human multipotent adipose-derived stem cells -- Cell sheet technology for tissue engineering: The self-assembly approach using adipose-derived stromal cells -- Differentiation of adipose-derived stem cells for tendon repair -- Adipose-derived stem cells for skin regeneration -- Adipose-derived stem cells for periodontal tissue regeneration.
  • Adult stem cells 2006
    Also available: Print – 2006
    Ectoderm -- Mesoderm -- Endoderm -- Extraembryonic and perinatal stem cells.
  • Basic Biology of Adult Stem Cells -- Molecular Mechanisms Regulating Adult Stem Cell Self-Renewal -- Maintenance of Adult Stem Cells: Role of the Stem Cell Niche -- The Emerging Role of microRNAs in Adult Stem Cells -- Expression and Function of Pluripotency Genes in Adult Stem Cells -- Adult Stem Cell Plasticity Revisited --Characterization of Adult Stem Cells -- Lineage Tracing of Tissue-Specific Stem Cells In Vivo -- Surrogate Measures of Adult Stem Cell Self-Renewal: The Neural Stem Cell Paradigm -- ABC Transporters, Aldehyde Dehydrogenase, and Adult Stem Cells -- Regulation of Life Span and Immortalization -- Regulation of Life Span in Adult Stem Cells -- The Cancer Stem Cell Paradigm.
  • Speakers at the symposium -- Stem cells in adult intestine / Feng, J., Sperka, T. -- Molecular mechanisms of muscle stem cell aging / Schwërer, S., Tümpel, S. -- Mechanism of functional alterations in hematopoietic stem cell aging / Morita, Y. -- The microenvironment, aging, and disease / Geiger, H. -- Sestrins in aging and metabolism / Tang, D., Tao S. -- Telomeres and stem cell aging / Felix, D.A. -- DNA damage and checkpoint responses in adult stem cells / Romanov, V., Shukla, A., Ju, Z.
  • "A reflection of the intense study of the effects of electromagnetic fields on living tissues that has taken place during the last several decades, this book discusses the theoretical and experimental evidence and considerations the effects of strong electromagnetic fields and/or electric pulses and their importance in medicine and biology. The authors present the basic techniques applied in electroporation and the advanced methods for creation of nanopores, highlighting their basic science and clinical applications. Topics include nano electroporation, classic electroporation, experimental evidence for electroporation of living cells, and electroporation for cancer and wound healing"--Provided by publisher.
  • 1. Cancer Stem Cells: A Revisitation of the "Anaplasia" Concept / Roberto Scatena -- 2. Stem Cells and Cancer Stem Cells: New Insights / Toru Kondo -- 3. Molecular Biology of Cancer Stem Cells / Oswaldo Keith Okamoto -- 4. Biomarkers of Cancer Stem Cells / Jun Dou and Ning Gu -- 5. Cancer Stem Cells and the Microenvironment / Alfonso Colombatti, Carla Danussi, Eliana Pivetta, and Paola Spessotto -- 6. Leukemia Stem Cells / Steven W. Lane and David A. Williams -- 7. Cancer Stem Cells and the Central Nervous System / Serdar Korur, Maria Maddalena Lino, and Adrian Merlo -- 8. Cancer Stem Cells and Glioblastoma Multiforme: Pathophysiological and Clinical Aspects / Akio Soeda, Mark E. Shaffrey, and Deric M. Park -- 9. Breast Cancer Stem Cells / Nuria Rodríguez Salas, Enrique González González, and Carlos Gamallo Amat -- 10. Colon Cancer Stem Cells / Ugo Testa -- 11. Liver Tumor-Initiating Cells/Cancer Stem Cells: Past Studies, Current Status, and Future Perspectives / Kwan Ho Tang, Stephanie Ma, and Xin-Yuan Guan -- 12. Pancreatic Cancer Stem Cells / Erica N. Proctor and Diane M. Simeone -- 13. Cancer Stem Cells and Renal Carcinoma / Benedetta Bussolati and Giovanni Camussi -- 14. Cancer Stem Cells: Proteomic Approaches for New Potential Diagnostic and Prognostic Biomarkers / Patrizia Bottoni, Bruno Giardina, and Roberto Scatena -- 15. Cancer Stem Cells: An Innovative Therapeutic Approach / Roberto Scatena, Patrizia Bottoni, Alessandro Pontoglio, Salvatore Scarà, and Bruno Giardina -- 16. Cancer Stem Cell and ATP-Binding Cassette: Which Role in Chemoresistance? / Andrea Silvestrini, Elisabetta Meucci, Giuseppe Ettore Martorana, Bruno Giardina, and Alvaro Mordente -- 17. Stem Cells and Cancer Stem Cells: Biological and Clinical Interrelationships / Cinzia Bagalà and Carlo Barone -- 18. Immunomodulatory Functions of Cancer Stem Cells / Tobias Schatton, Jennifer Y. Lin, and Markus H. Frank.
  • Also available: Print – 2012
  • Speakers at the symposium / Morita, M. -- Aging of the niche and the microenvironment and its role in stem cell aging / Geiger, H. -- Hematopoietic stem cell aging and cancer / Chen, Y., Ju, Z. -- DNA damage, checkpoint responses, and cell cycle control in aging stem cells / Kleinhans, K.N., Burkhalter, M.D. -- Hematopoietic stem cell aging and fate decision / Illing, A., Morita, Y. -- Stem cell therapy and stress response in pancreas and intestine -- Sperka, T., Omrani, O. -- Niche-stem cell interations and environmental influences / Tang, D. -- Stem cells and metabolism / Missios, P., Guachalla, L. -- Molecular mechanisms of muscle stem cell aging / Baig, A.H., Tümpel, S. -- Neural stem cells in development and aging / Schmidt-Strassburger, U.
  • Advances in stem cell research discusses recent advances in stem cell science, including therapeutic applications. This volume covers such topics as biomanufacturing iPS cells for therapeutic applications, techniques for controlling stem cell fate decisions, as well as current basic research in such areas as germ line stem cells, genomics and proteomics in stem cell research. It is a useful book for biology and clinical scientists, especially young investigators and stem cell biology students who are newly entering the world of stem cells research. The editors hope that the new knowledge and research outlined in this book will help contribute to new therapies for a wide variety of diseases that presently afflict humanity.
  • Also available: Print – 2011
    Roles of the TNF family in tissue homeostasis and normal development -- Novel aspects of signaling activation by the TNF family: novel aspects of the functions of the TRAFs and cIAPs -- Novel aspects of signaling activation by the TNF: ubiquitin modification of signaling proteins -- Novel aspects of signaling activation by the TNF family: TNF signaling and gene regulatory networks -- Roles of the TNF family in infectious diseases and interrelationship of the TNF family and pattern recognition receptor signaling: roles of the TNF family in infectious diseases -- Roles of the TNF family in infectious diseases and interrelationship of the TNF family and pattern recognition receptor signaling: interrelationship of the TNF family and pattern recognition receptor signaling -- Novel aspects of immune regulation by the TNF family -- Control of lymphocyte function and repertoire by the TNF family -- Advances in applying our knowledge to therapy: mechanisms of pathological and therapeutic effects of the TNF family -- Advances in applying our knowledge to therapy: rational design of new means for therapeutic modulation of function of the TNF family -- Roles of the TNF family in neuronal development, function, and pathology -- Cell death mechanisms controlled by the TNF family -- Contributors to abstracts of talks and posters at the 12th TNF Conference -- Abstracts of talks and posters presented at the 12th TNF Conference.
  • The Commitment of human cells to senescence / R. Holliday -- Evolutionary theories of aging can explain why we age / E. Le Bourg -- Control of cell replication during aging / A. Macieira-Coelho -- Cell senescence : role in aging and age-related diseases / J. Campisi, L. Robert, -- Aging of cell membranes : facts and theories / I. Zs.-Nagy -- Oxidative stress, mitochondrial dysfunction, and the mitochondria theory of aging / Y. Kong, S.E. Trabucco, H. Zhang -- Aging of connective tissues : experimental facts and theoretical considerations / J. Labat-Robert, L. Robert -- Aging of cell communication : loss of receptor function / L. Robert, T. Fulop -- On the immunological theory of aging / T. Fulop, J.M. Witkowski, G. Pawelec, C. Alan, A. Larbi -- Aging of the brain, dementias, role of infectious proteins : facts and theories / F. Morinet -- Aging as alteration / P.-A. Miquel -- Longevity and its regulation : centenarians and beyond / L. Robert, T. Fulop.
  • Introduction -- Oxidative stresses and ageing -- The role of mitochondria in the aging processes of yeast -- The retrograde response and other pathways of interorganelle communication in yeast replicative aging -- Chronological aging in Saccharomyces cerevisiae -- Aging and the survival of quiescent and non-quiescent cells in yeast stationary-phase cultures -- Maximising the yeast chronological lifespan -- Amino acid homeostasis and chronological longevity in Saccharomyces cerevisiae -- DNA damage and DNA replication stress in yeast models of aging -- Yeast aging and apoptosis -- Cellular homeostasis in fungi: impact on the aging process -- Genome-wide analysis of yeast aging -- Genetic approaches to aging in budding and fission yeasts: new connections and new opportunities -- Evolution of asymmetric damage segregaton: a modelling approach -- Cellular ageing and the actin cyoskeleton.
  • Print Material
  • Print Material
  • Print Material
  • Print Material
  • This book is a major update of novel targets in angiogenesis modulation, including pro- and anti-angiogenesis. There is in-depth coverage of preclinical and clinical methods and models, investigational status, and clinical applications. The impact of nanotechnology in advancing the applications of pro-and anti-angiogenesis strategies is also highlighted, along with stem cell and biotechnologies in research and development of angiogenesis modulating targets.
  • Animal cell culture 2015
    Animal cells are the preferred "cell factories" for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a℗ℓrecommended volume for all biotechnology libraries.
  • Apoptosis 2010
  • Also available: Print – 2012
  • Also available: Print – 2009
  • This book covers all of the diagnostic areas where FNAC is used today. This includes palpable lesions and lesions sampled using various radiological methods, and correlations with ancillary examinations detailed on an entity-by-entity basis. As well as being a complete atlas of the facts and findings important to FNAC, this atlas is a guide to diagnostic methods that optimize health care. The interaction of the cytologist or cytopathologist with other specialists (radiologists, oncologists and surgeons) involved in the diagnosis and treatment of patients with suspicious mass lesions is emphasized and illustrated throughout. ? With contributions from experts in the field internationally and abundant colour images Atlas of Fine Needle Aspiration Cytology provides a comprehensive and up-to-date guide to FNAC for pathologists, cytopathologists, radiologists, oncologists, surgeons and others involved in the diagnosis and treatment of patients with suspicious mass lesions.
  • Part 1. Distribution and arrangement of ICC in the GI tract -- Stomach -- Small Intestine -- Duodenum -- Colon -- Caecum -- Ileocaecal Junction -- ICC Found in the Submucosal Layer -- Introduction -- Part 2. Ultrastructural demonstration of ICC and the allied cells -- Ultrastructural Demonstration of ICC -- Signal Pathways Between Nerves and Muscles Not Mediated by ICC -- Issues for Future Studies.
  • Also available: Print – 2002
  • v. 2, 2014 ScienceDirect
    v.3, 2014 ScienceDirect
    Volume 1. Molecular mechanisms -- Volume 2. Role in general diseases -- Volume 3. Mitophagy.
  • This book covers the latest advances in the field of autophagy and cancer from the basics of the molecular machinery for autophagy induction and regulation up to current areas of interest, including modulation of autophagy and drug discovery for cancer prevention and treatment. The reader will be initially introduced to the historical context of autophagy, followed by a summary of the key players in autophagy, a discussion on the origin of autophagosomes, and an overview of signal transduction regulation of autophagy. The next chapters deal with the complex relationship between autophagy and tumorigenesis through in-depth discussions of how autophagy influences cell survival, apoptosis, inflammation, immunity, tumor microenvironment, metabolic stress, DNA damage, and genomic instability. The remaining chapters discuss how autophagy affects cancer cell response to therapy and how to best modulate autophagy for therapeutic benefit - what to target and how to target. We hope that this monograph will be an invaluable reference source for professionals, students, postdoctoral fellows, and senior scientists working in the fields of autophagy and cancer.
  • Also available: Print – 2009
  • Also available: Print – 2009
  • Also available: Print – 2009
  • Also available: Print – 2008
  • Bird's eye view of the bacterial landscape / Natividad Ruiz -- Visualizing the bacterial cell surface : an overview / Harald Engelhardt -- Purification of the outer membrane usher protein and periplasmic chaperone-subunit complexes from the P and type 1 pilus systems / Nadine S. Henderson and David G. Thanassi -- Experimental manipulation of the microbial functional amyloid called curli / Yizhou Zhou [and others] -- Visualization of gram-positive bacterial pili / Chungyu Chang [and others] -- Single cell microfluidic studies of bacterial motility / Adrien Ducret, Olivier Theodoly, and Tam Mignot -- Chromatographic analysis of the Escherichia coli polysialic acid capsule / Susan M. Steenbergen and Eric R. Vimr -- Analysis of exopolysaccharides in Myxococcus xanthus using confocal laser scanning microscopy / Wei Hu, Renate Lux, and Wenyuan Shi -- Assessment of multidrug efflux assemblies by surface plasmon resonance / Elena B. Tikhonova and Helen I. Zgurskaya -- Fluorescence microscopy and proteomics to investigate subcellular localization, assembly, and function of the type II secretion system / Tanya L. Johnson [and others] -- Pore formation by T3SS translocators : liposome leakage assay / Eric Faudry, Caroline Perdu, and Ina Attree -- Isolation of bacterial type IV machine subassemblies / Mayukh K. Sarkar [and others] -- Production and crystallization of bacterial type V secretion proteins / Hye-Jeong Yeo -- Assembly of bacterial outer membrane proteins / Jan Grijpstra, Martine P. Bos, and Jan Tommassen -- Outer membrane of gram-negative bacteria : lipid A isolation and characterization / Jessica V. Hankins [and others] -- Quantitative and qualitative preparations of bacterial outer membrane vesicles / Halima Chutkan [and others] -- In vitro peptidoglycan synthesis assay with lipid II substrate / Jacob Biboy, Nhat Khai Bui, and Waldemar Vollmer -- Extraction of cell wall-bound teichoic acids and surface proteins from Listeria monocytogenes / Filipe Carvalho [and others] -- Subfractionation and analysis of the cell envelope (lipo) polysaccharides of Mycobacterium tuberculosis / Anna E. Grzegorzewicz and Mary Jackson -- Protein disulfide bond formation in the periplasm : determination / Katleen Denoncin [and others] -- Using reporter genes and the Escherichia coli ASKA overexpression library in screens for regulators of the gram negative envelope stress response / Julia L. Wong, Stefanie L. Vogt, and Tracy L. Raivio -- Isolation of bacteria envelope proteins / Shu Quan [and others] -- Patch clamp electrophysiology for the study of bacterial ion channels in giant spheroplasts of E. coli / Boris Martinac [and others] -- Electrophysiological characterization of bacterial pore-forming proteins in planar lipid bilayers / Owen S. Mapingire, Beau Wager, and Anne H. Delcour.
  • pt. 1. Systems biology approach to [beta]-cells -- pt. 2. Imaging and sensors -- pt. 3. Genetics and proteomics -- pt. 4. Physiological, pharmaceutical and clinical applications and perspectives -- pt. 5. Mathematical modelling and numerical simulation.
  • This book offers clear, up-to-date guidance on how to report cytologic findings in cervical, vaginal and anal samples in accordance with the 2014 Bethesda System Update. The new edition has been expanded and revised to take into account the advances and experience of the past decade. A new chapter has been added, the terminology and text have been updated, and various terminological and morphologic questions have been clarified. In addition, new images are included that reflect the experience gained with liquid-based cytology since the publication of the last edition in 2004. Among more than 300 images, some represent classic examples of an entity while others illustrate interpretative dilemmas, borderline cytomorphologic features or mimics of epithelial abnormalities. The Bethesda System for Reporting Cervical Cytology, with its user-friendly format, is a must have for pathologists, cytopathologists, pathology residents, cytotechnologists, and clinicians.
  • 1. Integration, networking, and global biobanking in the age of new biology -- 2. The future of biobanking: a conceptual look at how biobanks can respond to the growing human biospecimen needs of researchers -- 3. Sustainability of biobanks in the future -- 4. Biobanking: the future of cell preservation strategies -- 5. Biobanking for personalized medicine -- 6. A global view of breast tissue banking -- 7. Biobanking of cerebrospinal fluid for biomarker analysis in neurological diseases -- 8. Biobanking in the twenty-first century: driving population metrics into biobanking quality -- 9. Challenges in developing a cancer oriented-biobank: experience from a 17 year-old cancer biobank in Sao Paulo, Brazil -- 10. China biobanking -- 11. Establishing an iso-compliant modern cancer-biobank in a developing country: a model for international cooperation -- 12. Nursing and biobanking -- 13. A data-centric strategy for modern biobanking -- 14. The importance of quality patient advocacy to biobanks: a lay perspective from Independent Cancer Patients Voice (ICPV), based in United Kingdom -- Index.
  • Also available: Print – 2015
    Consists of critical reviews and original research papers from the 2014 International Symposium on the "Biochemical Role of Eukaryotic Cell Surface Macromolecules". Topics covered include: · neurochemical and biochemical analysis of cell surface glycoconjugates · membrane skeletal organization · GPCRs and other receptors · biophysical approaches to study membrane interactions · glycoconjugate metabolism · dysregulation · molecular mechanisms involved in cell-cell and cell-matrix interaction · glycans in infectious and neurological diseases · cancer and glycosyltransferases as drug targets.
  • Also available: Print – 2012
    Glycomics: An Overview of the Complex Glycocode / Garima Gupta and Avadhesha Surolia -- Structural Glycomic Approaches to Molecular Recognition Events on Cell Surfaces / Koichi Kato -- ISCSM2011 Chondroitin Sulfate E-type Structure at Tumor Cell Surface Is Involved in Experimental Metastasis / Kazuyuki Sugahara and Shuji Mizumoto -- Chondroitin Sulfate-Specific Novel Hydrolase in Human / Shuhei Yamada -- Identification of Endothelial Cell Surface Carbohydrate-Binding Receptors by Carbohydrate Ligand Mimicry Peptides / Michiko N. Fukuda -- Lysosomal Enzyme Sorting Receptors - Where Did They First Appear in the Animal Kingdom? / Suryanarayanaraju Vegiraju, Suresh Koduru and Siva Kumar Nadimpalli -- Endoplasmic Reticulum-Targeted Bcl-2 Inhibitable Mitochondrial Fragmentation Initiates ER Stress-Induced Cell Death / B. C. Bhavya, Deepa Indira, Mahendra Seervi, Jeena Joseph and Praveen K. Sobhan, et al. -- Interactions Between Caveolin-1 and Sphingolipids, and Their Functional Relevance / Sandro Sonnino, Simona Prioni, Vanna Chigorno and Alessandro Prinetti -- Cell Membrane Repair Pathway Involves Sensing of Dynamics of Caveolae and Caspase-1 / Saumya S. Srivastava and M. V. Krishnasastry -- Angiogenic Response of Endothelial Cells to Fibronectin / V. B. S. Kumar, R. I. Viji, M. S. Kiran and Perumana R. Sudhakaran -- Lactosylceramide Synthase as a Therapeutic Target to Mitigate Multiple Human Diseases in Animal Models / Subroto Chatterjee and Nezar Alsaeedi -- Advanced FRET Methodologies: Protein-Lipid Selectivity Detection and Quantification / Fábio Fernandes, Manuel Prieto and Luís M. S. Loura -- Mechanism of GPCR-Directed Autoantibodies in Diseases / Hamiyet Unal, Rajaganapathi Jagannathan and Sadashiva S. Karnik -- Role of Membrane Cholesterol in Leishmanial Infection / Amitabha Chattopadhyay and Md. Jafurulla -- How Intact Is the Basement Membrane? Role of MMPs / S. Asha Nair, Sankar Jagadeeshan, Ramachandran Indu, Perumana R. Sudhakaran and M. R. Pillai -- Apoptosis of Breast Cancer Cells: Modulation of Genes for Glycoconjugate Biosynthesis and Targeted Drug Delivery / Subhash Basu, Rui Ma, Joseph R. Moskal, Manju Basu and Sipra Banerjee -- Altered Expression of Sialidases in Human Cancer / Taeko Miyagi, K. Takahashi, S. Moriya, K. Hata and K. Yamamoto, et al. -- Poly-ADP-Ribosylation of Vascular Endothelial Growth Factor and Its Implications on Angiogenesis / S. Binu, S. J. Soumya, V. B. S. Kumar and Perumana R. Sudhakaran -- Sphingolipid-Binding Domain in the Serotonin1A Receptor / Amitabha Chattopadhyay, Yamuna Devi Paila, Sandeep Shrivastava, Shrish Tiwari and Pushpendra Singh, et al. -- Withanolide D, Carrying the Baton of Indian Rasayana Herb as a Lead Candidate of Antileukemic Agent in Modern Medicine / Susmita Mondal, Saptarshi Roy, Rita Maity, Asish Mallick and Rajender Sangwan, et al. -- Engineered Glucose to Generate a Spectroscopic Probe for Studying Carbohydrate Biology / Ashish Tripathi, Vibha Singh, K. G. Aishwarya, Gopala Krishna Aradhyam and Srinivas Hotha -- Changes in Sialic Acid Content of Jelly Coat in Pesticide-Exposed Frog Eggs and Their Influence on Fertilization / H. P. Gurushankara, S. V. Krishnamurthy and V. Vasudev.
  • The regulation of gene expression -- The regulation of enzyme activity -- Structure and function of signal pathways -- Signaling by nuclear receptors -- G protein-coupled signal transmission pathways -- Intracellular messenger substance : second messengers -- Ser/Thr-specific protein kinases and protein phosphates -- Signal transmission via transmembrane receptors with tyrosine-specific protein kinase activity -- Signal transmission via ras proteins -- Intracellular signal transduction : the protein cascades of the MAP kinase pathways -- Membrane receptors with associated tyrosine kinase activity -- Other receptor classes -- Regulation of the cell cycle -- Malfunction of signaling pathways and tumorigenesis : oncogenes and tumor suppressor genes -- Apoptosis.
  • Theory of organelle biogenesis : a historical perspective / Barbara M. Mullock and J. Paul Luzio -- Protein coats as mediators of intracellular sorting and organelle biogenesis / Chris Mullins -- The role of proteins and lipids in organelle biogenesis in the secretory pathway / Thomas F.J. Martin -- Endoplasmic reticulum biogenesis : proliferation and differentiation / Erik Snapp -- The golgi apparatus : structure, function and cellular dynamics / Nihal Altan-Bonnet and Jennifer Lippincott-Schwartz -- Lysosome biogenesis and dynamics / Diane McVey Ward, Shelly L. Shiflett and Jerry Kaplan -- Nucleogenesis / Sui Huang -- Mitochondrial biogenesis / Danielle Leuenberger, Sean P. Curran and Carla M. Koehler -- The biogenesis and cell biology of peroxisomes in human health and disease / Stanley R. Terlecky and Paul A. Walton.
  • Cell Senescence Culturing Methods / Huaping Chen, Yuanyuan Li, and Trygve O. Tollefsbol -- Digital Image Analysis of Cells Stained with the Senescence-Associated b-Galactosidase Assay / Liran I. Shlush and Sara Selig -- Analysis of Biomarkers of Caloric Restriction in Aging Cells / Yuanyuan Li and Trygve O. Tollefsbol -- Cell Sorting of Young and Senescent Cells / Graeme Hewitt, Thomas von Zglinicki, and Joã̃o F. Passos -- Studying the Replicative Life Span of Yeast Cells / David A. Sinclair -- Methods for Creating Mutations in C. elegans That Extend Lifespan / Dayong Wang, Min Cao, Jessica Dinh, and Yuqing Dong -- Aging Studies in Drosophila Melanogaster / Yaning Sun, Jason Yolitz, Cecilia Wang, Edward Spangler, Ming Zhan, and Sige Zou -- The Use of Calorie Restriction Mimetics to Study Aging / Munehiro Kitada and Daisuke Koya -- Using Somatic-Cell Nuclear Transfer to Study Aging / Satoshi Kishigami, Ah Reum Lee, and Teruhiko Wakayama -- Induction of Cellular Senescence by Oncogenic RAS / Romi Gupta and Narendra Wajapeyee -- Methods of Cellular Senescence Induction Using Oxidative Stress / Zhe Wang, Dandan Wei, and Hengyi Xiao -- Methods of Testing Pharmacological Drugs Effects on Aging and Life Span in Mice / Vadimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski -- Mitochondria-Targeted Antiaging Gene Therapy with Adeno-associated Viral Vectors / Dejia Li and Dongsheng Duan -- Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration / Nadav Bleich Kimelman, Ilan Kallai, Dmitriy Sheyn, Wafa Tawackoli, Zulma Gazit, Gadi Pelled, and Dan Gazit -- Exometabolomic Mapping of Caenorhabditis elegans: A Tool to Noninvasively Investigate Aging / Robert J. Mishur, Jeffrey A. Butler, and Shane L. Rea -- Detecting Polymorphisms in Human Longevity Studies: HLA Typing and SNP Genotyping by Amplicon Sequencing / Gilberto Vargas-Alarcón and Carmina Flores-Domı́nguez -- Gel Electrophoresis-Based Proteomics of Senescent Tissues / Steven Carberry and Kay Ohlendieck -- Single-Cell Semiconductor Sequencing / Andrea B. Kohn, Tatiana P. Moroz, Jeffrey P. Barnes, Mandy Netherton, and Leonid L. Moroz -- Application of DNA Microarray Technology to Gerontological Studies / Kiyoshi Masuda, Yuki Kuwano, Kensei Nishida, and Kazuhito Rokutan -- Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells / Carmen M. Koch and Wolfgang Wagner -- Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging / Leonid L. Moroz and Andrea B. Kohn
  • Also available: Print – 2015
    In recent years, a significant amount of research has emerged connecting the link between alcohol and cancer. The field has rapidly advanced, especially since the complex connection between alcohol and cancer has several unique sub areas that are being investigated and this volume gives a comprehensive overview of these advancements.
  • This book fills in a gap in the NO literature. Recent progress in the field of NO-biology shows that NO is generated within distinct cell compartments, including specific plasma membrane regions, mitochondria, chloroplasts, peroxisomes, the Golgi-complex and intracellular membrane systems. NO synthesis plays specific roles in these compartments and, in turn, cell organelles also control intracellular NO levels. This monograph focuses on the roles played by the subcellular NO-signaling microdomains in the prokaryote-, fungus-, plant- and animal cells and shows how NO behaves as an intracellular signal in distinct cellular environments. This monograph also provides a summary of our knowledge on how NO synthesis came through evolution to be associated with organelles and subcellular compartments. Promotes the novel ideas that some functions of NO and its associations with subcellular units have been conserved during the evolution of the cell. A special chapter is dedicated to the biomedical relevance of subcellular NO synthesis, and this chapter also discusses the evidence that altered compartmentalization of NO-producing enzymes causes disease.
  • 2014 Knovel
    "Written by world-leading experts, this book focusses on the role of biomaterials in stem cell research and regenerative medicine. Emphasising basic principles and methodology, it covers stem cell interactions, fabrication technologies, design principles, physical characterisation and biological evaluation, across a broad variety of systems and biomaterials. Topics include: stem cell biology, including embryonic stem cells, IPS, HSC and progenitor cells; modern scaffold structures, including biopolymer, bioceramic, micro- and nanofiber, ECM and biohydrogel; advanced fabrication technologies, including computer-aided tissue engineering and organ printing; cutting-edge drug delivery systems and gene therapy techniques; medical applications spanning hard and soft tissues, the cardiovascular system and organ regeneration. With a contribution by Nobel laureate Shinya Yamanaka, this is a must-have reference for anyone in the field of biomaterials, stem cell biology and engineering, tissue engineering and regenerative medicine"--Provided by publisher.
  • Also available: Print – 2008
  • Also available: Print – 2008
  • Chapter 1. In vitro systems for the study of microtubule-based cell polarity in fission yeast -- Chapter 2. Microtubules, MAPs, and motor patterns -- Chapter 3. Self-organization of motors and microtubules in lipid-monolayered droplets -- Chapter 4. Reconstitution of microtubule-based motility using cell extracts -- Chapter 5. Building cells for quantitative, live-cell analyses of collective motor protein functions -- Chapter 6. Reconstituting cytoskeletal contraction events with biomimetic actin-myosin active gels -- Chapter 7. Building an artificial actin cortex on microscopic pillar arrays -- Chapter 8. Triggering actin polymerization in Xenopus egg extracts from phosphoinositide-containing lipid bilayers -- Chapter 9. Reconstituting geometry-modulated protein patterns in membrane compartments -- Chapter 10. Structural and functional studies of membrane remodeling machines -- Chapter 11. Building interconnected membrane networks -- Chapter 12. Using supported bilayers to study the spatiotemporal organization of membrane-bound proteins -- Chapter 13. Reconstituting ParA/ParB-mediated transport of DNA cargo -- Chapter 14. Cell-sized liposomes that mimic cell motility and the cell cortex -- Chapter 15. Reconstitution of cortical actin networks within water-in-oil emulsions -- Chapter 16. Engineering artificial cells by combining HeLa-based cell-free expression and ultrathin double emulsion template -- Chapter 17. Reconstitution of proteins on electroformed giant unilamellar vesicles -- Chapter 18. Reconstituting SNARE-mediated membrane fusion at the single liposome level.
  • An updated edition of the classic Methods in Cell Biology volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.
  • Pt.I Genetics and functional genomics : Mapping mutations in C. elegans / Eric J. Lambie -- Specialized chromosomes and their uses in caenorhabditis elegans / Martin R. Jones, Zoe Lohn, Ann M. Rose -- Genome engineering by transgene-instructed gene conversion in C. elegans / Valérie J.P. Robert, Jean-Louis Bessereau -- RNAi methods and screening : RNAi based high-throughput genetic interaction screening / Patricia G. Cipriani, Fabio Piano -- Dissection of genetic pathways in C. elegans / Zheng Wang, David R. Sherwood -- Pt.II Molecular biology and biochemistry : Transgenesis in C. elegans / Vida Praitis, Morris F. Maduro -- RNA processing in C. elegans / J. Jason Morton, Thomas Blumenthal -- Analysis of microRNA expression and function / Priscilla M. Van Wynsberghe, Shih-Peng Chan, Frank J. Slack, Amy E. Pasquinelli -- In situ hybridization of embryos with antisense RNA probes / Gina Broitman-Maduro, Morris F. Maduro -- Gene-centered regulatory network mapping / Albertha J.M. Walhout -- Affinity purification of protein complexes in C. elegans / Esther Zanin, Julien Dumont, RetoGassmann, Iain Cheeseman, Paul Maddox, Shirin Bahmanyar, Ana Carvalho, Sherry Niessen, John R. Yates III, Karen Oegema, Arshad Desai -- Pt.III Development : Cell indentification and cell lineage analysis / Claudiu A. Giurumescu, Andrew D. Chisholm -- The genetics and cell biology of fertilization / Brian D. Geldziler, Mathew R. Marcello, Diane C. Shakes, Andrew Singson -- Imaging embryonic morphogenesis in C. elegans / Jeff Hardin -- Molecular and genetic approaches for the analysis of C. elegans neuronal development / Dong Yan, Yishi Jin -- Methods for evaluating the caenorhabditis elegans dauer state : standard dauer-formation assay using synthetic daumones and proteomic analysis of O-GIcNAc modifications / Jeeyong Lee, Kwang-Youl Kim, Kyoe-Jin Joo, Keekyeong Kim, Pan-Young Jeong, Young-Ki Paik.
  • Calcium Handling in hiPSC-Derived Cardiomyocytes -- Myocardial Infarction and Heart Failure -- Embryonic Stem Cells/Pluripotent Stem Cells -- Human Induced Pluripotent Stem Cell (hiPSC)-Derived Cardiomyocytes as a Source for Cardiac Regeneration -- Spontaneous Cardiac Differentiation -- Mesoderm Enrichment by Wnt-Signaling Growth Factors -- Endoderm-Like Cell (END)-2 Co-culture Method -- Calcium Homeostasis in Cardiomyocytes -- Sarcoplasmic Reticulum (SR) Governs Maturity of Cardiomyocytes -- SR Junctional Proteins Play a Role in Calcium Flux Between Cytosol and SR -- Spatial and Temporal Ca2+ Wavefront Dictated by T-Tubule Structure -- IP3-Mediated Calcium Release Contributes to Whole-Cell Calcium Transients -- Calcium Handling Properties of hES-Derived Cardiomyocytes -- Calcium Handling Properties of hiPSC-Derived Cardiomyocytes -- iPSC-Derived Cardiomyocyte as a Potential Platform for Disease Modeling of the Impaired Calcium Handling-Related Syndrome -- Disease Modeling of Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT) -- Mitochondrial Dysfunction-Related Cardiomyopathy-Impaired SR Ca2+ Reuptake -- Measurement of Cytosolic [Ca2+] by Fluorescence Confocal Microscopy -- Materials -- Calibration of Fluo-3 -- Isolation of hiPSC- and hESC-Derived Cardiomyocytes -- Recording of Cytosolic [Ca2+] -- RT-qPCR of Calcium Handling Proteins.
  • Chapter 1. A Practical Guide to the Preparation of Ca2+ Buffers / Donald M. Bers, Chris W. Patton, Richard Nuccitelli -- Chapter 2. Photorelease Techniques for Raising or Lowering Intracellular Ca2+ / Robert Zucker -- Chapter 3. Making and Using Calcium-Selective Mini- and Microelectrodes / L. Hove-Madsen, S. Baudet, D.M. Bers -- Chapter 4. Construction, Theory, and Practical Considerations for using Self-referencing of Ca2+-Selective Microelectrodes for Monitoring Extracellular Ca2+ Gradients / Mark A. Messerli, Peter J.S. Smith -- Chapter 5. Practical Aspects of Measuring Intracellular Calcium Signals with Fluorescent Indicators / Joseph P.Y. Kao, Gong Li, Darryl A. Auston -- Chapter 6. Genetically Encoded Probes for Measurement of Intracellular Calcium / Michael Whitaker -- Chapter 7. Patch Clamp Methods for Studying Calcium Channels / David L. Armstrong, Christian Erxleben, Jody A. White -- Chapter 8. Nuclear Patch-Clamp Recording from Inositol 1,4,5-Trisphosphate Receptors / Taufiq Rahman, Colin W. Taylor -- Chapter 9. Confocal and Multiphoton Imaging of Intracellular Ca2+ / Godfrey Smith, Martyn Reynolds, Francis Burton, Ole Johan Kemi -- Chapter 10. The Use of Aequorins to Record and Visualize Ca2+ Dynamics: From Subcellular Microdomains to Whole Organisms / Sarah E. Webb, Kelly L. Rogers, Eric Karplus, Andrew L. Miller.
  • Calcium measurement methods 2010
    Springer Protocols
    Principles of the CA2+ homeostatic/signalling system / A. Verkhratsky and O.H. Petersen -- CA2+ recordings: Hardware and software (from microscopes to cameras) / E.C. Toescu and J. Graham -- CA2+ imaging: Principles of analysis and enhancement / F. Mammano and M. Bortolozzi -- Bioluminescent CA2+ indicators / L. Fedrizzi and M. Brini -- Monitoring calcium levels with genetically encoded indicators / O. Garaschuk and O. Griesbeck -- Intracellular calcium-sensitive microelectrodes / R.C. Thomas -- CA2+ caging and uncaging / S.H. Kim and M.K. Park -- CA2+ imaging of intracellular organelles: Endoplasmic reticulum / R. Blum, O.H. Petersen and A. Verkhratsky -- CA2+ imaging of intracellular organelles: Mitochondria / L. Núñez [and others] -- CA2+ imaging of dendrites and spines / K. Holthoff -- In vivo CA2+ imaging of the living brain using multi-cell bolus loading technique / G. Eichhoff [and others] -- CA2+ imaging of glia / C. Lohr and J.W. Deitmer.
  • Calcium signaling 2012
    Also available: Print – 2012
    "This volume ... [explores] topics ranging from the fundamental aspects of calcium signaling to its varied clinical implications. It presents comprehensive discussion of cutting-edge research alongside detailed analysis of critical issues, at the same time as setting out testable hypotheses that point the way to future scientific endeavors. The contributions feature material on theoretical and methodological topics as well as related subjects including mathematical modeling and simulations. They examine calcium signaling in a host of contexts, from mammalian cells to oocytes, zebrafish and plants. With much of interest to newcomers to the field as well as seasoned experts, this new publication is both wide-ranging and authoritative." --Excerpt from publisher's description.
  • Summary -- Introduction -- Direct evidence - the digital approach -- Fluorescence-based visualisation -- Aequorin-based measurements -- Measurement of calcium transport across membranes -- Molecular biology based approaches -- Manipulation of calcium -- Calcium-induced function -- Calcium signalling in cardiac myocytes -- Calcium signalling in red blood cells -- Perspective -- Calcium Signalling Methodology -- Non-linear and ultra high-speed imaging for explorations of the murine and human heart -- A system for optical high resolution screening of electrical excitable cells -- Concepts for optical high content screens of excitable primary isolated cells -- A primary culture system for sustained expression of a calcium sensor in preserved adult rat ventricular myocytes -- Calcium imaging of individual erythrocytes: Problems and approaches -- Calcium Signalling in Cardiac Myocytes -- Reduced Cardiac L-Type Ca²+ Current in Cav€ ₂ -/- Embryos Impairs Cardiac Development and Contraction With Secondary Defects in Vascular Maturation -- Overexpression of junctin causes adaptive changes in cardiac myocyte Ca²+ signaling -- Remodelling of Ca²+ handling organelles in adult rat ventricular myocytes during longterm culture -- Functional and morphological preservation of adult ventricular myocytes in culture by sub-micromolar cytochalasin D supplement -- Calcium Signalling in Red Blood Cells -- The non-selective voltage-activated cation channel in the human red blood cell membrane: reconciliation between two conflicting reports and further characterisation -- Ion channels in the human red blood cell membrane: Their further investigation and physiological relevance -- Prostaglandin E₂ activates channelmediated calcium entry in human erythrocytes: An indication for a blood clot formation supporting process -- Functional NMDA receptors in rat erythrocytes -- Stimulation of human red blood cells leads to Ca²+-mediated intercellular adhesion -- Lysophospatidic acid induced red blood cell aggregation in vitro -- Regulation of phosphatidylserine exposure in red blood cells -- Cation Channels in Erythrocytes - Historical and Future Perspective.
  • Annexinopathies / M.J. Hayes ... [et al.] -- Calpains and human disease / I. Bertipaglia and E. Carafoli -- Gelsolin and diseases / L. Spinardi and W. Witke -- Guanylate cyclase-activating proteins and retina disease / W. Baehr and K. Palczewski -- Pathologies involving the S100 proteins and rage / C.W. Heizmann ... [et al.] -- The calcium-sensing receptor: Physiology, pathophysiology and CaR-based therapeutics / E.M. Brown -- Physiological roles of the Ca2+/CaM-dependent protein kinase cascade in health and disease / J. Colomer and A.R. Means -- Calcium channelopathies: Voltage-gated calcium channels / P.J. Adams and T.P. Snutch -- TRP channels in disease / S.E. Jordt and B.E. Ehrlich -- Diseases associated with altered ryanodine receptor activity / W.J. Durham ... [et al.] -- Inositol 1,4,5-tripshosphate receptor, calcium signalling and Huntington's disease / I. Bezprozvanny -- SERCA pumps and human diseases / A. Hovnanian -- The plasma membrane calcium ATPase and disease / B.L. Tempel and D.J. Shilling -- Diseases involving the Golgi calcium pump / J. Vanoevelen ... [et al.] -- Calcium signalling and cancer cell growth / T. Capiod ... [et al.] -- Calcium misregulation and the pathogenesis of muscular dystrophy / F.W. Hopf ... [et al.] -- Calcium and cell death / A. Verkhratsky -- Calcium and cell death: The mitochondrial connection / P. Bernardi and A. Rasola -- Role of calcium in the pathogenesis of Alzheimer's disease and transgenic models / K.N. Green ... [et al.] -- Calcium and cardiomyopathies / E.G. Kranias and D.M. Bers -- Calcium signalling and calcium transport in bone disease / H.C. Blair ... [et al.].
  • Xenotransplantation of breast cancers -- Feeder layers: Co-culture with nonneoplastic cells -- Drug design and testing: Profiling of antiproliferative agents for cancer therapy using a cell-based methyl-[3h]-thymidine incorporation assay -- Transfection and DNA-mediated gene transfer -- Analysis of drug interactions -- Development of rituximab-resistant B-NHL clones: An in vitro model for studying tumor resistance to monoclonal antibody-mediated immunotherapy -- Proteomic evaluation of cancer cells: Identification of cell surface proteins -- Measuring gene expression from cell cultures by quantitative reverse-transcriptase polymerase chain reaction -- Expression analysis of homeobox genes in leukemia/lymphoma cell lines -- Flow cytometric DNA analysis of human cancers and cell lines -- Angiogenesis assays -- Cell migration and invasion assays -- Molecular breakpoint analysis of chromosome translocations in cancer cell lines by long distance inverse-PCR -- Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay -- Purification of annexin V and its use in the detection of apoptotic cells -- Real-time cytotoxicity assays -- Differential staining cytotoxicity assay: A review -- Cell sensitivity assays: The ATP-based tumor chemosensitivity assay -- Cell sensitivity assays: The MTT assay -- Cellular chemosensitivity assays: An overview -- Isolation of endothelial cells from human tumors -- Isolation of inflammatory cells from human tumours -- Establishment and culture of leukemia-lymphoma cell lines -- Isolation and culture of ovarian cancer cells and cell lines -- Isolation and culture of squamous cell carcinoma lines -- Isolation and culture of melanoma and naevus cells and cell lines -- Isolation and culture of colon cancer cells and cell lines -- Generation of lung cancer cell line variants by drug selection or cloning -- Quality assurance and good laboratory practice -- Elimination of mycoplasmas from infected cell lines using antibiotics -- Detecting mycoplasma contamination in cell cultures by polymerase chain reaction -- Cell culture contamination -- Cytogenetic analysis of cancer cell lines -- Online verification of human cell line identity by STRDNA typing -- Characterization and authentication of cancer cell lines: An overview -- Storage of cell lines -- Principles of cancer cell culture -- Cancer biology.
  • Also available: Print – 2011
    Immune infiltration in human cancer : prognostic significance and disease control / Wolf H. Fridman ... [et al.] -- Subversion and coercion : the art of redirecting tumor immune surveillance / John B. Mumm and Martin Oft -- STAT3 : a target to enhance antitumor immune response / Heehyoung Lee ... [et al.] -- Biology and clinical observations of regulatory T cells in cancer immunology / Michele W. Teng ... [et al.] -- Concepts and ways to amplify the antitumor immune response / Bent Rubin and Jean Edouard Gairin -- Angiogenesis and the tumor vasculature as antitumor immune modulators : the role of vascular endothelial growth factor and endothelin / Lana E. Kandalaft ... [et al.] -- Adoptive cellular therapy / Stephen A. Grupp and Carl H. June -- Dendritic cell subsets as vectors and targets for improved cancer therapy / Karolina Palucka ... [et al.] -- Identification of human idiotype-specific T cells in lymphoma and myeloma / Jinsheng Weng ... [et al.] -- Modulation of CTLA-4 and GITR for Cancer Immunotherapy / Francesca Avogadri ... [et al.] -- Immunobiology of cancer therapies targeting CD137 and B7-H1/PD-1 cosignal pathways / Shengdian Wang and Lieping Chen -- LAG-3 in cancer immunotherapy / Monica V. Goldberg and Charles G. Drake -- Immunologically active biomaterials for cancer therapy / Omar A. Ali and David J. Mooney -- Erratum : Identification of human idiotype-specific T cells in lymphoma and myeloma / Jinsheng Weng ... [et al.].
  • Cancer stem cells : historical perspectives and lessons from leukemia / Christopher R. Cogle -- Cancer stem cells in breast cancer / Jenny E. Chu and Alison L. Allan -- Cancer stem cells in brain cancer / Xin Wang, Chitra Venugopal, and Sheila K. Singh -- Cancer stem cells in colorectal cancer / Mauro Biffoni, Eros Fabrizi, and Lucia Ricci-Vitiani -- Cancer stem cells in pancreatic cancer / Jorge Dorado, Alicia G. Serrano, and Christopher Heeschen -- Cancer stem cells in prostate cancer / Paula Kroon ... [et al.] -- Cancer stem cells in melanoma / Ping Jin ... [et al.] -- Cancer stem cells in lung cancer / Jun Shen and Feng Jiang -- Cancer stem cells in ovarian cancer / Fang Fang ... [et al.] -- Cancer stem cells in hepatocellular cancer / Russell C. Langan and Itzhak Avital -- Cancer stem cells in head and neck cancer / Mark E.P. Prince and Samantha J. Davis -- Relationship between regulatory pathways in pluripotent stem cells and human tumors / Olga Gaidarenko and Yang Xu -- Influence of the embryonic microenvironment on tumor progression / Daniela Quail ... [et al.] -- The epithelial-to-mesenchymal transition and cancer stem cells / Jonas Fuxe -- Application of stem cell assays for the characterization of cancer stem cells / Pamela M. Willan and Gillian Farnie -- Zebrafish as a model to study stem cells in development, disease, and cancer / Viviana Anelli, Cristina Santoriello, and Marina C. Mione -- Imaging cancer stem cells / Paula Foster -- Mouse models for studying normal and cancer stem cells / David A. Hess -- Cancer stem cells and disease prognosis / Zeshaan A. Rasheed, Jeanne Kowalski, and William H. Matsui -- Mechanisms of radioresistance in cancer stem cells / Cleo Y-F Lee and Maximilian Diehn -- The role of ABC transporters in cancer stem cell drug resistance / Vera S. Donnenberg, Ludovic Zimmerlin, and Albert D. Donnenberg -- Resistance to endocrine therapy in breast cancer : are breast cancer stem cells implicated? / Ciara S. O'Brien ... [et al.] -- Future directions : cancer stem cells as therapeutic targets / Alysha K. Croker and Alison L. Allan -- Final thoughts : complexity and controversy surrounding the "cancer stem cell" paradigm / Craig Gedye, Richard P. Hill, and Laurie Ailles.
  • Also available: Print – 2012
    Lipid rafts, caveolae, and GPI linked proteins / Valerie L. Reeves, Candice M. Thomas, and Eric J. Smart -- Caveolae and the regulation of endocytosis / Anna L. Kiss -- Caveolin : role in cell signaling / Cecile Boscher and Ivan Robert Nabi -- Regulation of ENOS in caveolae / Chieko Mineo and Philip W. Shaul -- Recent developments in the interactions between caveolin and pathogens / Fabiana S. Machado ... [et al.] -- Caveolin and breast cancer : a new clinical perspective / Isabelle Mercier and Michael P. Lisanti -- Caveolin and prostate cancer progression / Michael R. Freeman, Wei Yang, and Dolores Di Vizio -- Caveolins and caveolae, roles in insulin signalling, and diabetes / Peter Strolfors -- Atherosclerosis, caveolae, and caveolin / Stephanos Pavlides ... [et al.] -- Caveolins and heart diseases / Mathivadhani Panneerselvam, Hemal H. Patel, and David M. Roth -- Caveolins and lung function / Nikolaos A. Maniatis ... [et al.].
  • Aging and cell aging : an introduction -- Cell cycle : the life cycle of a cell -- Theories and mechanisms of aging -- Selected age-related disorders.
  • Histopathology and grading of breast cancer / Magda Esebua -- Multicentric/multifocal breast cancer : overview, biology, and therapy / Federico Buggi ... [et al.] -- Immune system in breast cancer initiation and progression : role of epithelial to mesenchymal transition / Keith L. Knutson and Erik C. Radisky -- Remodelling of the extracellular matrix : implication for cancer / Thomas R. Cox and Janine T. Erler -- Biology and treatment of basal-like breast cancer / Bingchen Han ... [et al.] -- Re-excision after lumpectomy for breast cancer / Suzanne B. Coopey -- Novel anti-angiogenic therapies using naturally occurring and synthetic drugs to combat progestin-dependent breast cancer / Salman M. Hyder, Benford Mafuvadze, and Cynthia Besch-Williford -- New insights on estrogen receptor actions in hormone-responsive breast cancer cells by interaction proteomics / Concetta Ambrosino ... [et al.] -- Reprogramming breast cancer cells with embryonic microenvironments : insights from nodal signaling / Gina Kirsammer and Mary J.C. Hendrix - Metastatic determinants : breast tumour cells in circulation / Nisha Kanwar and Susan J. Done -- Breast cancer epigenetics : biomarkers and therapeutic potential / Nancy H. Nabilsi ... [et al.] -- Impact of centrosome abnormalities on breast cancer development and progression with a focus on targeting centrosomes for breast cancer therapy / Heide Schatten -- New perspective on cyclin D1 : beyond cell cycle regulation / Chenguang Wang, Timothy G. Pestell, and Richard G. Pestell -- Gene signatures of inflammatory breast cancer : epithelial plasticity and a cancer stem cell phenotype / Fredika M. Robertson ... [et al.] -- Integrated human mammary epithelial cell culture system for studying carcinogenesis and aging / Martha R. Stampfer, Mark A. LaBarge, and James C. Garbe -- New breast cancer treatment considerations a brief review of the use of genetically modified (attenuated) bacteria as therapy for advanced and metastatic breast cancer / Robert A. Kazmierczak ... [et al.].
  • Also available: Print – 2002
  • Also available: Print – 2010
    Early embryonic cell fate decisions in the mouse / Yojiro Yamanaka and Amy Ralston -- Nuclear architecture in stem cells / Kelly J. Morris, Mita Chotalia, and Ana Pombo -- Epigenetic regulation of pluripotency / Eleni M. Tomazou and Alexander Meissner -- Autosomal lyonization of replication domains during early mammalian development / Ichiro Hiratani and David M. Gilbert -- Preservation of genomic integrity in mouse embryonic stem cells / Peter J. Stambrook and Elisia D. Tichy -- Transcriptional regulation in embryonic stem cells / Jian Chien, Dominic Heng, and Huck Hui Ng -- Alternative splicing in stem cell self renewal and differentiation / David A. Nelles and Gene W. Yeo -- MicroRNA regulation of embryonic stem cell self renewal and differentiation / Collin Melton and Robert Blelloch -- Telomeres and telomerase in adult stem cells and pluripotent embryonic stem cells / Rosa M. Marión and Maria A. Blasco -- X chromosome inactivation and embryonic stem cells / Tahsin Stefan Barakat and Joost Gribnau -- Adult stem cells and their niches / Francesca Ferraro, Cristina Lo Celso, and David Scadden -- Adult stem cell differentiation and trafficking and their implications in disease / Ying Zhuge, Zhao-Jun Liu, and Omaida C. Velazquez -- Vertebrates that regenerate as models for guiding stem cells / Christopher L. Antos and Elly M. Tanaka -- Reprogramming of somatic cells to pluripotency / Masato Nakagawa and Shinya Yamanaka.
  • Also available: Print – 2009
  • Also available: Print – 2006
  • Part I: Starting the Cell Division Cycle -- 1 Escape from Cellular Quiescence -- 2 Interplay Between Cyclin-Dependent Kinases and E2F-Dependent Transcription -- 3 Regulation of Pre-RC Assembly: A Complex Symphony Orchestrated by CDKs -- Part II: Proliferation Under Duress -- 4 Mitotic Checkpoint and Chromosome Instability in Cancer -- 5 Mitotic Catastrophe -- 6 p53, ARF, and the Control of Autophagy -- Part III: Long-Term Proliferation -- 7 Regulation of Self-Renewing Divisions in Normal and Leukaemia Stem Cells -- 8 Maintenance of Telomeres in Cancer -- 9 The Senescence Secretome and Its Impact on Tumor Suppression and Cancer -- Part IV: Applications in Preventing and Treating Cancer -- 10 Cell Cycle Deregulation in Pre-neoplasia: Case Study of Barrett8217;s Oesophagus -- 11 Targeting Cyclin-Dependent Kinases for Cancer Therapy -- Index.
  • The ability of stem/progenitor cells to exit the cell cycle is essential for proper embryonic development, but the mechanisms governing cell cycle exit are still not fully understood. Correct regulation of G1 is critical for ensuring proper organismal development, tissue homeostasis, and tumor suppression. The Retinoblastoma (Rb) gene family is composed of three genes, Rb, p107, and p130, which are collectively thought to serve as essential regulators of the G1 phase of the cell cycle in most proliferating cell types. The Rb family has also been implicated in promoting the gene expression of several differentiation programs in different cell lineages. However, due to the overlapping and compensatory functions among the Rb family members, a complete understanding of how the Rb family controls the cell cycle and differentiation programs within a cell remains unclear. Furthermore, it has not yet been tested whether the Rb family members are the only mediators of these cellular processes, or if there are alternative mechanisms for cell cycle exit and differentiation in mammalian stem and progenitor cells. Here we tested the requirement for the Rb protein and its family members p107 and p130 in G1 arrest and differentiation in mammalian cells. We employed several strategies to conditionally remove the Rb family genes in entire embryos and in specific neural lineages, as well as multiple differentiation systems in vitro. We found that Rb family triple knock-out (TKO) mouse embryos survive until days 9-11 of gestation, with similar patterning and organogenesis as seen in wild type embryos. Strikingly, a number of TKO cells, including in the neural lineage, are able to exit the cell cycle in G1 and fully differentiate in vivo and in culture. This ability of TKO cells to arrest in G1 is associated with the repression of key cell cycle target genes for E2F and Myc transcription factors. Thus, G1 arrest can be achieved independently of Rb family members, illustrating the robustness of cell cycle regulatory networks during embryogenesis, and identifying a novel program that regulates cell cycle exit in G1 in mammalian cells.
  • Also available: Print – 2011
  • Also available: Print – 2006
  • [Publisher-supplied data] The ovary is a suitable organ for studying the processes of cell death. Cell death was first described in the rabbit ovary (Graaffian follicles), the phenomenon being called chromatolysis. To date, it is recognized that various forms of cell death (programmed cell death, apoptosis and autophagy) are essential components of ovarian development and function. Programmed cell death is responsable for the ovarian endowment of primordial follicles around birth; in the prepuberal and adult period, apoptosis is a basic mechanism by which oocytes are eliminated by cancer therapies and environmental toxicants; in the ovarian cycle, follicular atresia and luteal regression involve follicular cell apoptosis. Finally, abnormalities in cell death processes may lead to ovarian disease such as cancer and chemoresistance. In this book, after an introductory description of various forms of cell death and of the ovary development and function in mammals, the processes of cell death in ovarian somatic cells and oocytes are described at cytological, physiological and molecular levels and analyzed in the embryonic, prepuberal and adult ovary. A complex array of molecular pathways triggered by extrinsic and intrinsic signals able tor induce or suppress cell death in the same cell, according to cell type and ovary developmental stage, emerges. Physiological interactions with the axis hypothalamus-hypophysis as well as ovarian internal functional signal are also critically reviewed to explain the abortive development of follicles before the beginning of the ovarian cycle. The book conveys information useful to the updating of biologists and physicians who are interested to the ovary biology and functions. Hopefully it should provide also clues for stimulating novel experiments in the study of cell death in the mammalian ovary still at an early stage.
  • Beginning from centuries of anecdotal descriptions of cell death, such as those on the development of the midwife toad in 1842 by Carl Vogt, to modern-day investigations of cell death as a biological discipline, it has become accepted that cell death in multicellular organisms is a normal part of life. This book provides a comprehensive view of cell death, from its mechanisms of initiation and execution, to its implication in human disease and therapy. Physiological cell death plays critical roles in almost all aspects of biology, and the book details its roles in lymphocyte homeostasis, neuronal function, metabolism, and the DNA damage response.?When physiological cell death goes awry, diseases can arise, and cancer is presented as a central paradigm for the consequences of derangements in the interplay between cell survival and cell death. At the same time, the potential promise of targeted therapies aimed at interdicting cell death machineries are also discussed extensively. The molecular mechanisms that underlie apoptotic cell death are illustrated from the perspectives of both the intrinsic, mitochondrial apoptotic pathway and the extrinsic, death receptor pathway. Key players in these pathways, such as the Bcl2 family proteins, cytochrome c, Apaf-1, caspases, death receptor adapter proteins, and inhibitor of apoptosis proteins, are presented from both functional and structural angles. Until only a few years ago, programmed cell death has been considered essentially synonymous with apoptosis. However, we now know that programmed cell death can also take other forms such as necrosis or necroptosis, and to this end, the mechanisms that underlie programmed necrosis in development and host defense are illustrated. The past twenty plus years have seen an incredible growth of research in cell death, with one breakthrough after another, and the legacy still goes on with constant new surprises and findings. Long live cell death!
  • Defects in cell death pathways promote tumor development and progression, with potentially devastating consequences for cancer patients. Greater understanding of the defects occurring in cancer cells, and the unique characteristics of tumors which can make them vulnerable to cell death stimuli, offers tremendous opportunities for developing novel and effective anti-cancer therapies. In Cell Death in Cancer Biology and Treatment leading experts in the field provide a wealth of up-to-date knowledge regarding the molecular mechanisms and cell biological processes that control cell death. Each chapter also highlights recent advances in the translation of basic research findings into clinical trials. Beginning and established investigators alike will benefit from the thorough presentations of the most promising avenues for future development of cell death-based, anti-cancer strategies and agents. The volume begins with a detailed description of many of the cell death defects that have been identified in human tumor specimens. The unique bioenergetics of cancer cells, and the influence of the tumor microenvironment, autophagy, and cellular microRNAs on cancer cell death are then discussed, along with current progress in targeting these distinctive features and processes. Additional chapters describe recent advances, and the therapeutic benefits of targeting DNA repair pathways, protein chaperones, sphingolipid signaling, Bcl-2 family members, IAPs, death receptor signaling, the proteasome, and survival signaling emanating from the PI3K/AKT and RAS/RAF/MEK/ERK pathways. Finally, recent discoveries are presented regarding interactions between the immune system and dying cancer cells and the potential for optimizing these interactions to maximize anti-cancer activities. In summary, Cell Death in Cancer Biology and Treatment will be a valuable resource for scientists interested in cutting-edge understanding of aberrant cell death in cancer cells, and the multitude of innovative molecular targeting approaches that are actively being pursued to achieve selective activation of cell death in human malignancies.
  • Pt. 1. Cell fusion -- 1. Yeast mating, a model system for studying cell and nuclear fusion / Casey A. Ydenberg, Mark D. Rose -- 2. Cell fusion in the filamentous fungus, neurospora crassa / André Flei€ner, Anna R. Simonin, N. Louise Glass -- 3. Gametic cell adhesion and fusion in the uicellular alga chlamydomonas / Nedra F. Wilson -- 4. Cell Fusion in caenorhabditis elegans / Scott Alper, Benjamin Podbilewicz -- 5. Myoblast fusion in drosophila / Susan M. Abmayr, Shufei Zhuang, and Erika R. Geisbrecht -- 6. Mammalian fertilization is dependent on multiple membrane fusion events / Paul M. Wassarman, Eveline S. Litscher -- 7. Molecular control of mammalian myoblast fusion / Katie M. Jansen, Grace K. Pavlath -- 8. Placenta trophoblast fusion / Berthold Huppertz, Marcus Borges -- 9. Macrophage fusion / Agnès Vignery -- Pt. 2. Cell fusion assays -- 10. Cell fusion assays for yeast mating pairs / Eric Grote -- 11. Ultrastructural analysis of cell fusion in yeast / Alison E. Gammie -- 12. Isolation and in vitro binding of mating type plus fertilization tubules from chlamydomonas / Nedra F. Wilson -- 13. Optical imaging of cell fusion and fusion proteins in caenorhabditis elegans / Star Ems, William A. Mohler -- 14. Ultrastructural imaging of cell fusion in caenorhabditis elegans / Star Ems, William A. Mohler -- 15. Live imaging of drosophila myoblast fusion / Brian E. Richardson, Karen Beckett, Mary K. Baylies -- 16. Ultrastructural Analysis of myoblast fusion in drosophila / Shiliang Zhang, Elizabeth H. Chen -- 17. Genomic approach to myoblast fusion in drosophila / Beatriz Estrada, Alan M. Michelson -- 18. Mesenchymal cell fusion in the sea urchin embryo / Paul G. Hodor, Charles A. Ettensohn -- 19. Sperm-egg fusion assay in mammals / Naokazu Inoue, Masaru Okabe -- 20. Quantitative assays for cell fusion / Jessica H. Shinn-Thomas, Victoria L. Scranton, William A. Mohler -- 21. Fusion assays and models for the trophoblast / Sascha Drewlo, ... [et al.] -- 22. Methods to fuse macrophages in vitro / Agnès Vignery.
  • Also available: Print – 2011
    1. Introduction / Thomas Dittmar and Kurt S. Zänker -- 2. Heterochronic control of AFF-1-mediated cell-to-cell fusion in C. elegans / Lilach Friedlander-Shani and Benjamin Podbilewicz -- 3. Role of SNAREs in membrane fusion / Bhanu P. Jena -- 4. Molecular and cellular mechanisms of mammalian cell fusion / Xiaofeng Zhou and Jeffrey L. Platt -- 5. Membrane fusions during mammalian fertilization / Bart M. Gadella and Janice P. Evans -- 6. Trophoblast fusion / Berthold Huppertz and Martin Gauster -- 7. Macrophage fusion and multinucleated giant cells of inflammation / Amy K. McNally and James M. Anderson -- 8. Molecular mechanisms of myoblast fusion across species / Adriana Simionescu and Grace K. Pavlath -- 9. Cell-fusion-mediated reprogramming : pluripotency or transdifferentiation? : implications for regenerative medicine / Daniela Sanges, Frederic Lluis, and Maria Pia Cosma -- 10. Cell fusion and tissue regeneration / Manuel Álvarez-Dolado and Magdalena Martínez-Losa -- 11. Dendritic cell-tumour cell fusion vaccines / Walter T. Lee.
  • Also available: Print – 2011
    Introduction / Thomas Dittmar and Kurt S. Zänker -- Horizontal Gene Transfers with or without Cell Fusions in All Categories of the Living Matter / Joseph G. Sinkovics -- Class III Viral Membrane Fusion Proteins / Marija Backovic and Theodore S. Jardetzky -- Human Trophoblast in Trisomy 21: A Model for Cell - Cell Fusion Dynamic Investigation / André Malassiné, Guillaume Pidoux, Pascale Gerbaud, Jean Louis Frendo and Danièle Evain-Brion -- Cell Fusion and Hyperactive Osteoclastogenesis in Multiple Myeloma / Franco Silvestris, Sabino Ciavarella, Sabino Strippoli and Franco Dammacco -- Cell Fusion Hypothesis of the Cancer Stem Cell / Xin Lu and Yibin Kang -- Expression of Macrophage Antigens by Tumor Cells / Ivan Shabo and Joar Svanvik -- Leukocyte-Cancer Cell Fusion: Initiator of the Warburg Effect in Malignancy? / Rossitza Lazova, Ashok Chakraborty and John M. Pawelek -- Cell Fusion, Drug Resistance and Recurrence CSCs / Christa Nagler, Kurt S. Zänker and Thomas Dittmar -- The Role of Microvesicles in Malignancies / Erna Pap.
  • Molecular beacons: fluorescent probes for detection of endogenous mRNAs in living cells -- Second-harmonic imaging of collagen -- Visualizing calcium signaling in cells by digitized wide-field and confocal fluorescent microscopy -- Multifluorescence labeling techniques and confocal laser scanning microscopy on lung tissue -- Evaluation of confocal microscopy system performance -- Quantitative analysis of atherosclerotic lesion composition in mice -- Applications of microscopy to genetic therapy of cystic fibrosis and other human diseases -- Laser scanning cytometry: principles and applications -- Near-clinical applications of laser scanning cytometry -- Laser capture microdissection -- Analysis of asbestos-induced gene expression changes in bronchiolar epithelial cells using laser capture microdissection and quantitative reverse transriptase-polymerase chain reaction -- New approaches to fluorescence in situ hybridization -- Microarray image scanning -- Near-field scanning optical microscopy in cell biology and cytogenetics -- Porosome: the fusion pore revealed by multiple imaging modalities -- Secretory vesicle swelling by atomic force microscopy -- Imaging and probing cell mechanical properties with the atomic force microscopy -- Reflection contrast microscopy: the bridge between light and electron microscopy -- Three-dimensional analysis of single particles by electron microscopy: sample preparation and data acquisition -- Three-dimensional reconstruction of single particles in electron microscopy: image processing -- A new microbiopsy system enables rapid preparation of tissue for high-pressure freezing.
  • Cell mechanics 2007
    Also available: Print – 2007
  • The migrating cell / Entschladen, F., Zänker, K.S. -- Stem cell migration in health and disease / Dittmar, T. ... [et al.] -- Leukocyte motility and human disease / Cooper, K., Nuzzi, P., Huttenlocher, A. -- Coordination of leukocyte polarity and migration / Martín-Cófreces, N.B., Serrador, J.M., Sánchez-Madrid, F. -- Positioning phosphoinositide 3-kinase in chemokine and antigen-dependent T-lymphocyte navigation mechanisms / Ward, S.G. -- Migration of functionally specialized T-helper cells : TFH cells, Th17 cells, and FoxP3+ T cells / Kim, C.H. -- ADAMs and ectodomain proteolytic shedding in leukocyte and tumour cell migration / Ager, A., Knäuper, V., Poghosyan, Z. -- Guided tour of cell migration : signals and pathways / Ratke, J., Lang, K. -- Regulation of the E-cadherin adhesion complex in tumor cell migration and invasion / Menke, A., Giehl, K. -- The cytoskeletal connection : understanding adaptor proteins / Ziegler, W.H. -- Locomotor force generation by myosins / Jbireal, J.M.A., Entschladen, F., Zänker, K.S.
  • Cancer Morbidity and mortality result from invasive and metastatic spread. Advances in the field have highlighted the central role of cell motility during the dynamic and transient process of tumor invasion and metastasis. This book presents an overview of these studies.
  • Sect. I. Biophysical chemistry, metabolism, second messengers, and ultrastructure. Biophysical chemistry of physiological solutions / Jeffrey c. Freedman -- Physiological structure and function of proteins / Matthew R. Pincus -- Cell membranes / Jeffrey C. Freedman -- Ionophores in planar lipid bilayers / Jeffrey C. Freedman -- Cell structure / Michael S. Forbes -- Signal transduction and second messengers / Aldebaran M. Hofer -- Calcium as an intracellular second messenger: mediation by calcium-binding proteins / John R. Dedman and Marcia A. Kaetzel -- Sect. II. Membrane potential, transport physiology, pumps, and exchangers. Diffusion and permeability / Nicholas Sperelakis and Jeffrey C. Freedman -- Origin of resting membrane potentials / Nicholas Sperelakis -- Gibbs-Donnan equilibrium potentials / Nicholas Sperelakis -- Mechanisms of carrier-mediated transport: facilitated diffusion, cotransport and countertransport / Steven M. Grassl -- Active ion transport by ATP-driven ion pumps / Robert A. Farley -- Ca2+ -ATPases / Tracy J. Pritchard, Istvan Edes and Evangelia G. Kranias -- Na+ -Ca2+ exchange currents / John H.B. Bridge, Natalia S. Torres and Michela Ottolia -- Intracellular chloride regulation / Francisco J. Alvarez-Leefmans -- Osmosis and regulation of cell volume / Clive M. Baumgarten and Joseph J. Feher -- Intracellular pH regulation / Robert W. Putnam -- Sect. III. Membrane excitability and ion channels. Cable properties and propagation of action potentials / Nicholas sperelakis -- Electrogenesis of membrane excitability / Nicholas Sperelakis -- Patch-clamp techniques / Laura Conforti -- Structure and mechanism of voltage-gated ion channels / Simon Rock Levinson and William A. Sather -- Biology of gap junctions / Richard D. Veenstra -- Regulation of cardiac ion channels by cyclic nucleotide-dependent phosphorylation / Gordon M. Wahler and Nicholas Sperelakis -- Direct regulation of ion channels by GTP-binding proteins / Atsushi Inanobe and Yoshihisa Kurachi -- Developmental changes in ion channels / Takeshi Kobayashi ... [et al.] -- Regulation of ion channel localization and activity through interactions with the cytoskeleton / Stephen Lambert -- Why are so many ion channels mechanosensitives? / Catherine E. Morris -- Sect. IV. Ion channels as targets for toxins, drugs, and genetic diseases. Ion channels as targets for toxins / Kenneth M. Blumenthal -- Ion channels as targets for drugs / Seth Robey, Kevin J. Sampson and Robert S. Kass -- Inherited diseases of ion transport / Robert A. Farley -- Sect. V. Synaptic transmission and sensory transduction. Ligand-gated ion channels / Kenneth R. Tovar and Gary L. Westbrook -- Synaptic transmission / Janusz B. Suszkiw -- Excitation-secretion coupling / Nicole Gallo-Payet and Marcel Daniel Payet -- Stimulus-response coupling in metabolic sensor cells / Stan Misler -- Cyclic nucleotide-gated ion channels / Anita L. Zimmerman -- Sensory receptors and mechanotransduction / Andrew S. French and Päivi H. Torkkeli -- Acoustic transduction / Daniel C. Marcus -- Visual transduction / Anita L. Zimmerman -- Gustatory and olfactory sensory transduction / Stephen D. Roper -- Infrared sensory organs / Stephen D. Roper and Michael S. Grace -- Electroreceptors and magnetoreceptors / Timothy C. Tricas and Bruce A. Carlson -- Sect. VI. Muscle and other contractile systems. Skeletal muscle excitability / Nicholas Sperelakis, Judith Heiny and Hugo gonzalez-Serratos -- Cardiac action potentials / Gordon M. Wahler -- Smooth muscle excitability / Neil D. Detweiler ... [et al.] -- Excitation-contraction coupling in skeletal muscle / Judith A. Heiny and Gerhard Meissner -- Contraction of muscles: mechanochemistry / Richard J. Paul -- Flagella, cilia, actin- and centrin-based movement / Kenneth W. Foster -- Electrocytes of electric fish / Anthony L. Gotter, Marcia A. Kaetzel and John R. Dedman -- Sect. VII. Protozoa and bacteria. Physiological adaptations of protists / Michael Levandowsky -- Physiology of prokaryotic cells / Dennis W. Grogan -- Sect. VIII. Specialized processes: photosynthesis and bioluminescence. Photosynthesis / Darrell Fleischman -- bioluminescence / J. Woodland Hastings.
  • Cell senescence as both a dynamic and a static phenotype / Andrew R.J. Young, Masako Narita, and Masashi Narita -- Senescence regulation by mTOR / Vjekoslav Dulic -- Senescence regulation by the p53 protein family / Yingjuan Qian and Xinbin Chen -- Markers of cellular senescence / Amancio Carnero -- Biomarkers of cell senescence assessed by imaging cytometry / Hong Zhao and Zbigniew Darzynkiewicz -- Cytofluorometric assessment of cell cycle progression / Ilio Vitale, Mohamed Jemaà, Lorenzo Galluzzi, Didier Metivier, Maria Castedo, and Guido Kroemer -- Quantification of cell cycle-arresting proteins / Oliver Kepp, Isabelle Martins, Laurie Menger, Mickaël Michaud, Sandy Adjemian, Abdul Qader Sukkurwala, Lorenzo Galluzzi, and Guido Kroemer -- Colorimetric detection of senescence-associated [beta] galactosidase / Koji Itahana, Yoko Itahana, and Goberdhan P. Dimri -- Chemiluminescent detection of senescence-associated [beta] galactosidase / Vinicius Bassaneze, Ayumi Aurea Miyakawa, and José Eduardo Krieger -- Detection of the senescence-associated secretory phenotype (SASP) / Francis Rodier -- Unbiased characterization of the senescence-associated secretome using SILAC-based quantitative proteomics / Juan Carlos Acosta, Ambrosius P. Snijders, and Jesús Gil -- Detection of senescence-associated heterochromatin foci (SAHF) / Katherine M. Aird and Rugang Zhang -- Monitoring DNA damage during cell senescence / Glyn Nelson and Thomas von Zglinicki -- Assessment and quantification of telomerase enzyme activity / Michelle F. Maritz, Laura A. Richards, and Karen L. MacKenzie -- Methods for the assessment of telomere status / Asako J. Nakamura -- Detection of nuclear envelope alterations in senescence / Clea Bárcena, Fernando G. Osorio, and José Maria Pérez Freije -- Measuring reactive oxygen species in senescent cells / João F. Passos, Satomi Miwa, and Thomas von Zglinicki -- Quantification of protein carbonylation / Nancy B. Wehr and Rodney L. Levine -- Assays for the measurement of lipid peroxidation / Ana Cipak Gasparovic, Morana Jaganjac, Branka Mihaljevic, Suzana Borovic Sunjic, and Neven Zarkovic -- Raman spectroscopy for the detection of AGEs/ALEs / J. Renwick Beattie, John J. McGarvey, and Alan W. Stitt -- Monitoring oncogenic B-RAF-induced senescence in melanocytes / Sieu L. Tran and Helen Rizos -- Methods to investigate the role of SIRT1 in endothelial senescence / Bo Bai and Yu Wang -- Monitoring nutrient signaling through the longevity protein p66SHC1 / Sofia Chiatamone Ranieri and Giovambattista Pani -- Profiling the metabolic signature of senescence / Florian M. Geier, Silke Fuchs, Gabriel Valbuena, Armand M. Leroi, and Jacob G. Bundy -- Genome-wide RNAi screening to identify regulators of oncogene-induced cellular senescence / Narendra Wajapeyee, Sara K. Deibler, and Michael R. Green -- Integrated approach for monitoring cell senescence / Tatiana V. Pospelova, Zhanna V. Chitikova, and Valery A. Pospelov -- Robust multiparametric assessment of cellular senescence / Clara Correia-Melo, Diana Jurk, and João F. Passos -- Assessing chronological aging in bacteria / Stavros Gonidakis and Valter D. Longo -- Assessing organismal aging in the filamentous fungus Podospora anserina / Heinz D. Osiewacz, Andrea Hamann, and Sandra Zintel -- Assessing chronological aging in Saccharomyces cerevisiae / Jia Hu, Min Wei, Mario G. Mirisola, and Valter D. Longo -- Assessing aging and senescent decline in Caenorhabditis elegans : cohort survival analysis / Eirini Lionaki and Nektarios Tavernarakis -- High-throughput and longitudinal analysis of aging and senescent decline in Caenorhabditis elegans / Eirini Lionaki and Nektarios Tavernarakis -- Assessing senescence in Drosophila using video tracking / Reza Ardekani, Simon Tavaré, and John Tower -- Assessing vascular senescence in zebrafish / Sandra Donnini, Antonio Giachetti, and Marina Ziche.
  • Also available: Print – 2015
    The book considers signaling events from the zygote embryo through to the blastocyst with relevant data from embryonic stem (ES) cells, including dialogue with the extracellular environment and with the maternal tract during the implantation process. Application of the knowledge described to improve the success of human and animal assisted conception is considered where appropriate, but the focus is largely on fundamental rather than applied cell/molecular biology, as this is the area that has historically been neglected. While the general features of metabolism during preimplantation development are well established, especially in terms of nutrient requirements, uptake and fate, remarkably little is known about early embryo signaling events, intracellular or intercellular, between individual embryos in vitro or with the female reproductive tract in vivo. This contrasts with the wealth of information on cell signaling in somatic cells and tissues, as a glance at any textbook of biochemistry illustrates. This lack of information is such that our understanding of the molecular cell biology of early embryos -- a prerequisite to defining the mechanisms which regulate development at this critical stage of the life cycle -- is seriously incomplete. This volume is the first to address this issue by describing the current state of knowledge on cell signaling during mammalian early embryo development and highlighting priority areas for research.
  • Heme oxygenase-1 and carbon monoxide in vascular regulation / Stefan W. Ryter and Augustine M.K. Choi -- Tumor necrosis factor-[alpha]/receptor signaling through the Akt kinase / Osman Nidai Ozes ... [et al.] -- Protein modifications by nitric oxide and reactive nitrogen species / Harry Ischiropoulos -- Redox signaling in hypoxic pulmonary vasoconstriction / E. Kenneth Weir ... [et al.] -- cGMP-dependent protein kinase in regulation of the perinatal pulmonary circulation / Usha Raj ... [et al.] -- Glutamate receptor activation in the pathogenesis of acute leg injury / Sami I. Said -- Carboxyl methylation of small GTPases and endothelial cell function / Sharon Rounds, Elizabeth O. Harrington, and Qing Lu -- Pressure-induced inflammatory signaling in lung endothelial cells / Wolfgang M. Kuebler -- Regulation of endothelial barrier function: contributions of the transcellular and paracellular pathways / Dolly Mehta, Richard D. Minshall, and Asrar B. Malik -- Sphingolipid signaling: implications for vascular biology / Margaret M. Harnett -- Regulations of cytokine signaling / Bao Q. Vuong ... [et al.] -- Cell signaling by vasoactive agents / Barry L. Fanburg ... [et al.] -- Reactive oxygen species and cell signaling in lung ischemia / Aron B. Fisher -- Pulmonary vascular barrier regulation by thrombin and Edg receptors / Jeffrey R. Jacobson and Joe G.N. Garcia -- Second-messenger signaling in lung capillaries / Kaushik Parthasarathi -- Plasma membrane-to-nucleus calcium signaling / Giles E. Hardingham -- Signaling by mitochondria / Navdeep S. Chandel -- Pro-inflammatory signaling by endothelial focal complexes in lung / Sunita Bhattacharya -- Hydrogen peroxide as intracellular messenger: production, target, and elimination / Sue Goo Rhee ... [et al.] -- Calcium-inhibited adenylyl cyclase (AC6) controls endothelial cell barrier function / Troy Stevens.
  • Part 1. Regulatory. -- Regulation of Cell Product Manufacturing and Delivery: A United States Perspective / R.W. Lindblad -- The Regulatory Situation for Academic Cell Therapy Facilities in Europe / I. Slaper-Cortenbach, M. Scott, D. Herrmann, M. Introna and K. Theunissen, et al. -- A Regulatory System for Cell and Tissue Therapies: Proposed Approach in Australia / A. Farrugia -- Part 2. GMP Facility Design. -- University of Minnesota - Molecular and Cellular Therapeutics (MCT) / D.H. McKenna -- University of Pittsburgh Cancer Institute - Hematopoietic Stem Cell Laboratory (HSC Lab)/Immunological Monitoring and Cellular Products Laboratory (IMCPL) / D.L. Griffin, A.D. Donnenberg and T.L. Whiteside / Baylor College of Medicine - Center for Cell and Gene Therapy (CAGT) / A. Gee -- Design of a New GMP Facility - Lessons Learned / A. Gee -- Part 3. Professional Cell Therapy Standards. -- AABB Cell Therapy Standards / Z.M. Szczepiorkowski and E. Nunes -- Professional Standards for Cellular Therapies: Foundation for the Accreditation of Cellular Therapy (FACT) / P.I. Warkentin -- Part 4. Facility Operations -- Standard Operating Procedures / C.G. Lindgren -- Staffing, Training, and Competency / D.M. Kadidlo -- Cleaning Procedures / A. Gee and D.L. Lyon -- Environmental Monitoring / A. Gee and D.L. Lyon -- Supply Management / A. Gee and C.M. Rooney -- Facility Equipment / D.L. Griffin -- Quality / J.W. Atkins -- Product Manufacturing / A. Gee -- Product Review, Release, and Administration / N.H. Collins -- Use of a Facility Master File to Facilitate Regulatory Submissions for Cell Therapy Products / E.J. Read and H.M. Khuu.
  • Also available: Print – 2006
    Current views of the embryonic and neural stem cell : cell replacement or molecular repair? / Roya Sabetrasekh ... [et al.] -- Developing novel cell sources for transplantation in Parkinson's disease / Nicolaj S. Christophersen ... [et al.] -- Neural transplantation in the nonhuman primate model of Parkinson's disease / Kimberly B. Bjugstad and John R. Sladek, Jr. -- Cell-based therapy for Huntington's disease / Claire M. Kelly, Stephen B. Dunnett, and Anne E. Rosser -- Use of bone marrow stem cells as therapy for behavioral deficits in rodent models of Huntington's disease / Gary L. Dunbar, Justin D. Oh-Lee, and Laurent Lescaudron -- Human neuroteratocarcinoma cells as a neural progenitor graft source for cell transplantation in stroke / Cesario V. Borlongan ... [et al.] -- Therapeutic applications of bone marrow-derived stem cells in neurologic injury and disease / C. Dirk Keene ... [et al.] -- Cell therapy for models of pain and traumatic brain injury / Mary Eaton and Jacqueline Sagen -- The use of sertoli cells in neural transplantation / Dwaine F. Emerich, Cesario V. Borlongan, and Craig Halberstadt -- The choroids plexus : a novel graft source for neural transplantation / Cesario V. Borlongan ... [et al.] -- Progress and challenges in immunoisolation for CNS cell therapy / Christopher G. Thanos and Dwaine F. Emerich -- Evidence-based methodology for advancing neural reconstruction / Stephen Polgar -- Hematopoietic cell therapy for brain repair / Martina Vendrame and Alison E. Willing -- Stem cells and regenerative medicine : commercial and pharmaceutical implications / L. Eduardo Cruz and Silvia P. Azevedo.
  • Stroke remains one of the main causes of death and disability worldwide. Effective therapy for stroke recovery remains an unmet need. Much hope and promises are placed on cell-based therapies. The aim of this book is to provide focused yet comprehensive reviews on the current state of cell-based approaches in the treatment of stroke. The topics covered include experimental data on functional outcome after intravascular and intracerebral delivery of cells in stroke animals, followed by translational chapters which will rely heavily on the use of different imaging modalities in the tracking of cells. The last and most challenging part will describe the early phase of clinical studies, providing guidelines for future research and clinical applications. Clearly and concisely written, this text will be a useful resource for neurologists, radiologists, and neuroscientists interested in cell transplantation as a therapeutic strategy for stroke patients.
  • Age-related macular degeneration: clinical findings, histopathology, and imaging techniques / Zarbin, M.A.; Casaroli-Marano, R.P.; Rosenfeld, P.J. -- General pathophysiology in retinal degeneration / Wert, K.J.; Lin, J.H.; Tsang, S.H. -- Juvenile-onset macular degeneration and allied disorders / North, V.; Gelman, R.; Tsang, S.H. -- Diagnosis and complementary examinations / Menghini, M.; Duncan, J.L. -- Stem cells for retinal repair / Stern, J.; Temple, S. -- Differentiation of pluripotent stem cells into retinal pigmented epithelium / Croze, R.H.; Clegg, D.O. -- Neural retinal regeneration with pluripotent stem cells / Ramsden, C.M.; Powner, M.B.; Carr, A.-J.F.; Smart, M.J.K.; da Cruz, L.; Coffey, P.J. -- Stem cells, retinal ganglion cells and glaucoma / Sluch, V.M.; Zack, D.J. -- Stem cells : immunology and immunomodulation / Tena, A.; Sachs, D.H. -- Biochemical restoration of aged human Bruch's membrane : experimental studies to improve retinal pigment epithelium transplant survival and differentiation / Sugino, I.K.; Sun, Q.; Cheewatrakoolpong, N.; Malcuit, C.; Zarbin, M.A. -- Approaches to cell delivery : substrates and scaffolds for cell therapy / Kundu, J.; Michaelson, A.; Baranov, P.; Young, M.J.; Carrier, R.L. -- Microdevice-based cell therapy for age-related macular degeneration / Lu, B.; Tai, Y.-C.; Humayun, M.S. -- Cell and gene therapy / Rao, R.C.; Zacks, D.N. -- Cellular manufacturing for clinical applications / Sheu, J.; Klassen, H.; Bauer, G. -- Regulatory issues in cell-based therapy for clinical purposes / Casaroli-Marano, R.P.; Tabera, J.; Vilarrodona, A.; Trias, E.
  • Cell-cell channels 2006.
    Cell-cell channels and their implications for cell theory / Frantisek Baluska, Dieter Volkmann, and Peter W. Barlow -- Mating cell-cell channels in conjugating bacteria / Elisabeth Grohmann -- The tetrahymena conjugation junction / Eric S. Cole -- Cytoplasmic bridges in volvox and its relatives / Harold J. Hoops, Ichiro Nishii, and David L. Kirk -- Vegetative hyphal fusion in filamentous fungi / Nick D. Read and M. Gabriela Roca -- Plasmodesmata: cell-cell channels in plants / Yoselin Benitez Alfonso, Laurence Cantrill, and David Jackson -- Sieve-pore plugging mechanisms / Aart J.E. van Bel -- Actin and myosin VIII in plant cell-cell channels / Jozef Samaj ... [et al.] -- Cell-cell communication in wood / John R. Barnett -- TMV movement protein targets cell-cell channels in plants and prokaryotes: possible roles of tubulin- and FtsZ-based cytoskeletons / Manfred Heinlein -- Viral movement proteins induce tubule formation in plant and insect cells / Jan W.M. van Lent and Corinne Schmitt-Keichinger -- Cell-cell movements of transcription factors in plants / Alexis Maizel -- Gap junctions: cell-cell channels in animals / Fabio Mammano -- Tunneling nanotubes: membranous channels between animal cells / Hans-Hermann Gerdes and Amin Rustom -- Cytoplasmic bridges as cell-cell channels of germ cells / Sami Ventelä -- Fusome as a cell-cell communication channel of drosophila ovarian cyst / Jean-René Huynh -- Cytonemes as cell-cell channels in human blood cells / Svetlana Ivanovna Galkina .. [et al.] -- Paracellular pores in endothelial barriers / Luca Manzi and Gianfranco Bazzoni -- Channels across endothelial cells / Radu V. Stan -- Molecular transfers through transient lymphoid cell-cell channels / Mary Poupot, Julie Gertner, and Jean-Jacques Fournié -- Cell-cell transport of homeoproteins: with or without channels / Alain Joliot and Alain Prochiantz -- Virological synapse for cell-cell spread of viruses / Eduardo Garcia and Vincent Piguet -- Cell-cell fusion: transient channels leading to plasma membrane merger / William A. Mohler.
  • Cell cycle activation in neurons: the final exit of brain-morpho-dysregulation / Thomas Arendt -- Paved with good intentions: the link between cell cycle and cell death in the mammalian central nervous system / Yan Yang and Karl Herrup -- The role of presenilins in the cell cycle and apoptosis / Mervyn J. Monteiro -- Cell cycle activation and cell death in the nervous system / Zsuzsanna Nagy -- Cell cycle and chromosome segregation defects in Alzheimer's disease / Huntington Potter -- Alzheimer disease-a new beginning or a final exit? / Mark E. Obrenovich ... [et al.] -- From cell cycle activation to the inhibition of the WNT pathway: a hypothesis of how neurons die in response to B-Amyloid / Agata Copani ... [et al.].
  • Print Material
  • Also available: Print – 2014
    Cellular and Molecular Control of Neuronal Migration provides an up-to-date collection of reviews on the molecular and cellular principles of neuronal migration in the mammalian brain. Over the last decades a rich catalogue of signaling molecules controlling neuronal migration has been compiled, and within this book an international panel of experts provides up-to-date discussions of the state of knowledge how these distinct signaling pathways regulate various aspects of neuronal migration. This book introduces the reader to the latest discoveries and concepts of neuronal migration enabled through the application of most sophisticated methods and cutting edge experimental approaches. Cellular and Molecular Control of Neuronal Migration also provides an update on the underlying cellular and molecular basis of neurodevelopmental migration disorders in human patients for all interested neuroscientists and clinicians.
  • Also available: Print – 2007
    Physics of Rapid Cooling and Its Implications for Cryoimmobilization of Cells / J. Dubochet -- Cryopreparation Methods for Electron Microscopy of Selected Model Systems / K. McDonald -- Cryopreparation Methodology for Plant Cell Biology / M. W. Hess -- Correlative Light and Electron Microscopy of Early Caenorhabditis elegans Embryos in Mitosis / T. Muller-Reichert -- Understanding Microtubule Organizing Centers by Comparing Mutant and Wild-Type Structures with Electron Tomography / E. T. O'Toole -- Whole-Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography / J. L. Hoog -- Electron Microscopy of Archaea / C. E. Robertson -- Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography / B. J. Marsh -- Visualization of Membrane-Cytoskeletal Interactions During Plant Cytokinesis / M. S. Otegui -- Electron Tomographic Methods for Studying the Chemical Synapse / C. P. Arthur -- Using Electron Microscopy to Understand Functional Mechanisms of Chromosome Alignment on the Mitotic Spindle / B. F. McEwen -- Electron Microscopic Analysis of the Leading Edge in Migrating Cells / T. Svitkina -- Imaging Actomyosin In Situ / K. A. Taylor -- Electron Tomography of Bacterial Chemotaxis Receptor Assemblies / P. Zhang -- How to "Read" a Vitreous Section / J. Dubochet -- Single-Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction / I. I. Serysheva -- Electron Microscopy of Microtubule-Based Cytoskeletal Machinery / A. Hoenger -- Reconstructing the Endocytotic Machinery / Y. Cheng -- 3D Immunolocalization with Plastic Sections / M. K. Morphew -- Electron Microscopy Analysis of Viral Morphogenesis / A. Pelchen-Matthews -- Electron Tomography of Immunolabeled Cryosections / M. S. Ladinsky -- Visualizing Macromolecules with Fluoronanogold: From Photon Microscopy to Electron Tomography / T. Cheutin -- Markers for Correlated Light and Electron Microscopy / G. E. Sosinsky -- Localizing Specific Elements Bound to Macromolecules by EFTEM / R. D. Leapman -- Localization of Protein Complexes by Pattern Recognition / C. Best -- The Application of Energy-Filtered Electron Microscopy to Tomography of Thick, Selectively Stained Biological Samples / J. C. Bouwer -- Optimization of Image Collection for Cellular Electron Microscopy / P. Mooney -- Future Directions for Camera Systems in Electron Microscopy / N. Xuong -- Structure Determination In Situ by Averaging of Tomograms / F. Forster -- Methods for Image Segmentation in Cellular Tomography / K. Sandberg -- Database Resources for Cellular Electron Microscopy / M. E. Martone.
  • Also available: Print – 2010
  • 1. Human Evolution and Physical Exercise: The Concept of Being "Born to Run" / Livio Luzi -- 2. Cell Morphology and Function: The Specificities of Muscle Cells / Anna Maestroni -- 3. The Cell Membrane of the Contractile Unit / Gianpaolo Zerbini -- 4. Gene Polymorphisms and Athletic Performance / Ileana Terruzzi -- 5. Nutrients and Whole-Body Energy Metabolism: The Impact of Physical Exercise / Stefano Benedini -- 6. Mitochondrial and Non-mitochondrial Studies of ATP Synthesis / Roberto Codella -- 7. Excessive Nutrients and Regional Energy Metabolism / Gianluca Perseghin -- 8. Muscle Biopsy To Investigate Mitochondrial Turnover / Rocco Barazzoni -- 9. Introduction to the Tracer-Based Study of Metabolism In Vivo / Andrea Caumo and Livio Luzi -- 10. Physical Activity and Inflammation / Raffaele Di Fenza and Paolo Fiorina -- 11. The HPA Axis and the Regulation of Energy Balance / Francesca Frigerio -- 12. Physical Exercise in Obesity and Anorexia Nervosa / Alberto Battezzati and Simona Bertoli -- 13. Physical Exercise and Transplantation / Valentina Delmonte, Vincenzo Lauriola, Rodolfo Alejandro and Camillo Ricordi -- 14. The Baboon as a Primate Model To Study the Physiology and Metabolic Effects of Exercise / Francesca Casiraghi, Alberto Omar Chavez, Nicholas Musi and Franco Folli.
  • Introduction : homeostasis and cellular physiology -- Diffusion and permeability -- Osmotic pressure and water movement -- Electrical consequences of ionic gradients -- Ion channels -- Passive electrical properties of membranes -- Generation and propagation of the action potential -- Ion channel diversity -- Electrochemical potential energy and transport processes -- Passive solute transport -- Active transport -- Synaptic physiology I -- Synaptic physiology II -- Molecular motors and the mechanism of muscle contraction -- Excitation-contraction coupling in muscle -- Mechanics of muscle contraction.
  • "All biological phenomena emerge from an intricate interconnection of multiple processes occurring at different levels of organization: namely, at the molecular, the cellular and the tissue level, see Figure 1. These natural levels can approximately be connected to a microscopic, mesoscopic, and macroscopic scale, respectively. The microscopic scale refers to those processes that occur at the subcellular level, such as DNA synthesis and duplication, gene dynamics, activation of receptors, transduction of chemical signals, diffusion of ions and transport of proteins. The mesoscopic scale, on the other hand, can refer to cell-level phenomena, such as adhesive interactions between cells or between cells and ECM components, cell duplication and death and cell motion. The macroscopic scale finally corresponds to those processes that are typical of multicellular behavior, such as population dynamics, tissue mechanics and organ growth and development. It is evident that research in biology and medicine needs to work in a multiscale fashion. This brings many challenging questions and a complexity that can not be addressed in the classical way, but can take advantage of the increasing collaboration between natural and exact sciences (for more detailed comments the reader is referred to [90, 262]). On the other hand, the recent literature provides evidence of the increasing attention of the mathematical, statistical, computational and physical communities toward biological and biomedical modeling, consequence of the successful results obtained by a multidisciplinary approach to the Life Sciences problems"-- Provided by publisher.
  • Part I. Centrosomes in Reproduction -- 1. Centriole Duplication and Inheritance in Drosophila melanogaster / Tomer Avidor-Reiss, Jayachandran Gopalakrishnan, Stephanie Blachon and Andrey Polyanovsky -- 2. Sperm Centrioles and Their Dual Role in Flagellogenesis and Cell Cycle of the Zygote: Structure, Function, and Pathology / Hector E. Chemes -- 3. Centrosomal Functions and Dysfunctions in Cat Spermatozoa / Pierre Comizzoli and David E. Wildt -- 4. Nuclear-Centrosome Relationships During Fertilization, Cell Division, Embryo Development, and in Somatic Cell Nuclear Transfer Embryos / Heide Schatten and Qing-Yuan Sun -- 5. Human Centrosomal Dynamics During Gametogenesis, Fertilization, and Embryogenesis and Its Impact on Fertility: Ultrastructural Analysis / A. Henry Sathananthan -- 6. Asymmetric Centrosome Behavior in Stem Cell Divisions / Therese M. Roth, Yukiko M. Yamashita and Jun Cheng -- Part II. Cell and Molecular Biology of Centrosomes -- 7. Functional Associations Between the Golgi Apparatus and the Centrosome in Mammalian Cells / Breanne Karanikolas and Christine Sütterlin -- 8. Many Pathways to Destruction: The Role of the Centrosome in, and Its Control by Regulated Proteolysis / Harold A. Fisk -- 9. Regulation of the Centrosome Cycle by Protein Degradation / Suzanna L. Prosser and Andrew M. Fry -- 10. Molecular Links Between Centrosome Duplication and Other Cell Cycle-Associated Events / Kenji Fukasawa -- 11. Regulation of Centrosomes by Cyclin-Dependent Kinases / Rose Boutros -- Part III. Centrosome Abnormalities in Cancer -- 12. Disruption of Centrosome Duplication Control and Induction of Mitotic Instability by the High-Risk Human Papillomavirus Oncoproteins E6 and E7 / Nina Korzeniewski and Stefan Duensing -- 13. Centrosomes, DNA Damage and Aneuploidy / Chiara Saladino, Emer Bourke and Ciaran G. Morrison -- 14. Centrosome Regulation and Breast Cancer / Zeina Kais and Jeffrey D. Parvin -- 15. The Role of Centrosomes in Multiple Myeloma / Benedict Yan and Wee-Joo Chng -- 16. Centrosomal Amplification and Related Abnormalities Induced by Nucleoside Analogs / Ofelia A. Olivero -- 17. Mechanisms and Consequences of Centrosome Clustering in Cancer Cells / Alwin Krämer, Simon Anderhub and Bettina Maier -- Part IV. Centrosomes in Other Systems -- 18. Re-evaluation of the Neuronal Centrosome as a Generator of Microtubules for Axons and Dendrites / Peter W. Baas and Aditi Falnikar -- 19. Centrosomes and Cell Division in Apicomplexa / Leandro Lemgruber, Marek Cyrklaff and Freddy Frischknecht -- 20. The Centrosome Life Story in Xenopuslaevis / Jacek Z. Kubiak and Claude Prigent -- 21. Role of the MTOC in T Cell Effector Functions / Martin Poenie, Laura Christian, Sarah Tan and Yuri Sykulev -- 22. Thoughts on Progress in the Centrosome Field / Jeffrey L. Salisbury.
  • Centrosome & centriole 2015
    This new volume of Methods in Cell Biology looks at methods for analyzing centrosomes and centrioles. Chapters cover such topics as methods to analyze centrosomes, centriole biogenesis and function in multi-ciliated cells, laser manipulation of centrosomes or CLEM, analysis of centrosomes in human cancers and tissues, proximity interaction techniques to study centrosomes, and genome engineering for creating conditional alleles in human cells.
  • Also available: Print – 2001
  • At the forefront of life sciences today is the emerging discipline of chembiomolecular science. This new term describes the integration of the frontier fields of chemical biology, chemistry, and pharmacology. Chembiomolecular science aims to elucidate new biological mechanisms as potential drug targets and enhance the creation of new drug therapies. This book comprises the proceedings of the Uehara Memorial Foundation Symposium 2011, which focused on the most recent advances in chembiomolecular science made by leading experts in the field. The book is divided into three main topics. The first is the chemical approach to understanding complex biological systems on a molecular level using chemical compounds as a probe. The second describes the biological approach used to develop new lead drug compounds. The third focuses on the biological system that serves as the potential drug target, the beginning step in the process of developing new drugs. Replete with the latest research, the book will draw the attention of all scientists interested in the synergies between chemistry and biology to elucidate life on a molecular level and to promote drug discovery. Ultimately, the book helps promote the understanding of biological functions at the molecular level and create new pharmaceuticals that can contribute to improving human health.
  • Print Material
    v. 1. Collagen, basal laminae elastin. --v.2. Glycosaminoglycans and proteoglycans. --v.3. Structural organization and function of the matrix.
  • Also available: Print – 2006
  • Chromatin protocols. 2nd ed. 2009
    Springer Protocols
    Preparation of chromatin assembly extracts from preblastoderm Drosophila embryos / Edgar Bonte and Peter B. Becker -- Analysis of reconstituted chromatin using a solid-phase approach / Raphael Sandaltzopoulos and Peter B. Becker -- In vivo chromatin decondensation assays : molecular genetic analysis of chromatin unfolding characteristics of selected proteins / Sherry L. Winter, Philip Wong, and Mark G. Alexandrow -- DNA methyltransferase probing of chromatin structure within populations and on single molecules / Carolina Pardo ... [et al.] -- Visualization of the expression of HMGN nucleosomal binding proteins in the developing mouse embryo and in adult mouse tissues / Takashi Furusawa and Michael Bustin -- Drug-induced premature chromosome condensation (PCC) protocols : cytogenetic approaches in mitotic chromosome and interphase chromatin / Eisuke Gotoh -- Analysis of DNA topology in yeast chromatin / Randall H. Morse -- Preparation and analysis of uniquely positioned mononucleosomes / Daria A. Gaykalova ... [et al.] -- Monitoring DNA breaks in optically highlighted chromatin in living cells by laser scanning confocal microscopy / Michael J. Kruhlak, Arkady Celeste, and André Nussenzweig -- Methods to study transcription-coupled repair in chromatin / Hélène Gaillard, Ralf Erik Wellinger, and Andrés Aguilera -- Cytometric analysis of DNA damage : phosphorylation of histone H2AX as a marker of DNA double-strand breaks (DSBs) / Toshiki Tanaka ... [et al.] -- Detection of DNA damage induced by topoisomerase II inhibitors, gamma radiation, and crosslinking agents using the comet assay / Lori A. Hazlehurst -- Analysis of genomic aberrations using comparative genomic hybridization of metaphase chromosomes / Melanie Carless -- In vitro replication assay with mammalian cell extracts / Wasia Rizwani and Srikumar P. Chellappan -- Investigation of genomic methylation status using methylation-specific and bisulfite sequencing polymerase chain reaction / Melanie Carless -- Biochemical analysis of arginine methylation in transcription / Marc Tini, Hina Naeem, and Joseph Torchia -- In vitro histone demethylase assays / Kenji Kokura and Jia Fang -- Histone chaperone as coactivator of chromatin transcription : role of acetylation / Shrikanth S. Gadad ... [et al.] -- Histone deacetylase activity assay / Zhigang Yuan ... [et al.] -- In vitro and in vivo assays for studying histone ubiquitination and deubiquitination / Ling Zhai, Heui-Yun Joo, and Hengbin Wang -- Detection of histone H3 phosphorylation in cultured cells and tissue sections by immunostaining / Jaya Padmanabhan -- Chromatin immunoprecipitation assays : analyzing transcription factor binding and histone modifications in vivo / Smitha Pillai, Piyali Dasgupta, and Srikumar P. Chellappan -- ChIP on Chip assays : genome-wide analysis of transcription factor binding and histone modifications / Smitha Pillai and Srikumar P. Chellappan -- ChIP-on-Chip analysis methods for Affymetrix tiling arrays / Sean J. Yoder and Steven A. Enkemann -- Non-radioactive assay methods for the assessment of telomerase activity and telomere length / Partha P. Banerjee and Shankar Jagadeesh -- Detecting ATM-dependent chromatin modification in DNA damage and heat shock response / Sandeep Misri, Shruti Pandita, and Tej K. Pandita.
  • Early embryonic cell types such as the zygote, blastomeres of the preimplantation embryo, and embryonic stem (ES) cells have powerful chromatin remodeling activities that facilitate DNA-dependent processes such as transcription and DNA repair. Chd1l encodes a chromatin remodeling factor and is more highly expressed in the inner cell mass (ICM) compared to the whole blastocyst. Chd1l expression is developmentally regulated during a time course of preimplantation development and is present in ES cells. Intriguing expression patterns suggested Chd1l could be a novel regulator of DNA-dependent processes in early developmental cell types. This dissertation describes research undertaken to address the role of Chd1l in chromatin remodeling in the preimplantation embryo and in ES cells. Reducing Chd1l protein to nearly undetectable levels in ES cells reveals that Chd1l is dispensable for ES cell viability, proliferation, and pluripotent morphology. Global gene expression patterns are unaltered by Chd1l knock-down. Chd1l is also dispensable for expression patterns of lineage markers associated with the formation of the primary germ layers in differentiating embryoid bodies. Zygote-stage embryos injected with Chd1l-MO arrest prior to the morula stage. Knock-down by the MO was confirmed at the transcript levels by microfluidic qPCR, and the arrest phenotype was partially rescued upon co-injection of Chd1l mRNA and Chd1l-MO. ES cells are known to have stringent and unique pathways to repair DNA damage to prevent mutation and genomic instability from arising in the organism. Reduction of Chd1l in ES cells confers resistance to induced DNA damage, a finding that contrasts with another study that used a differentiated cell line. Apoptosis induced by over-expression of Chd1l occurs specifically in ES cells and not in their differentiated counterparts. This switch in the effect of Chd1l over-expression during differentiation suggests that the function of Chd1l is very different in ES cells than in differentiated somatic cells. DNA repair through non-homologous end joining (NHEJ) is particularly critical in the zygote to repair the paternal genome. Differentiated somatic cells, but not ES cells also rely heavily on NHEJ. Biochemical evidence supports the involvement of Chd1l in NHEJ. Deficient DNA repair could underlie the Chd1l arrest phenotype. Therefore, Chd1l functions minimally, or not at all, in regulating gene expression and contributes to the DNA damage response in a developmental stage-specific and/or cell type-specific manner.
  • Bacterial chromosomes are generally ~1000 times longer than the cells in which they reside, and concurrent replication, segregation, and transcription/translation of this crowded mass of DNA poses a challenging organizational problem. At first, it was assumed that DNA is organized randomly inside the cell, much like a bowl of spaghetti. Furthermore, it was argued that segregation of this disordered mass was not carried out by a mitotic machinery as is found in eukaryotes, but rather the by-product of cell growth. Hence, the canonical description of bacterial DNA was that of an unstructured polymer diffusing randomly. Recent advances in cell imaging technology, however, have revealed that the bacterial nucleoid is reliably oriented and highly organized within the cell. Furthermore, the speed of segregation is inconsistent with the old model driven by cell growth. Here, I use a screening approach to identify parS, a 100bp piece of DNA previously found to be anchored to the old cell pole, as the Caulobacter centromere. In direct contrast to other published models, parS segregates ahead of all other sequences, including the origin of replication (Cori), and moving parS away from Cori causes segregation -- but not replication -- to be delayed. The segregation of parS requires the ATPase activity of the ParA protein, and current work is aimed at understanding the molecular details of this process. All of these data point to the presence of an active, mitotic-like machine that drives chromosome segregation in Caulobacter. I also show that the circular Caulobacter chromosome is oriented in relation to the parS anchor site at the old cell pole, so that changing the genomic position of parS causes the entire chromosome to rotate within the cell. Finally, I find that the chromosome is organized into a dense core running down the length of the cell, and I arrive at two possible models for the large-scale structure of this core. Thus, this work forms part of an emerging view of the chromosome as a highly organized structure that is kept in place by simple yet active mechanisms, and whose segregation is insured by a complex dedicated machine.
  • Cilia. Part A-B pt. A-B, 2013
    pt. B ScienceDirect
  • Plant cell calcium, past and future / Anthony J. Trewavas -- Calcium signalilng and homeostasis in nuclei / Christian Mazars .. [et al.] --Interactions between calcium and ROP signaling regulate pollen tube tip growth / Augusta Jamin and Zhenbiao Yang -- Calcium, mechanical signaling, and tip growth / Won-Gyu Choi, Sarah J. Swanson, and Simon Gilroy -- Calcium signals in the control of stomatal movements / Alex A.R. Webb and Fiona C. Robertson -- Stimulus perception and membrane excitation in unicellular alga Chlamydomonas / Kenjiro Yoshimura -- Cyclic nucleotide gated channels (CNGCs) and the generation of Ca2+ signals / Wei Ma and Gerald A. Berkowitz -- Annexins / Anuphon Laohavisit and Julia M. Davies -- Structure and function of CDPK : a sensor responder of calcium / Yohsuke Takahashi and Takeshi Ito -- Elucidation of calcium-signaling components and networks / Irene S. Day and A.S.N. Reddy -- Decoding of calcium signal through calmodulin : calmodulin-binding proteins in plants / Liqun Du [and others] / The CBL-CIPK network for decoding calcium signals in plants / Oliver Batistic [and others].
  • In the newly updated edition of Comprehensive Cytopathology, a team of international experts provides criteria and techniques in diagnosis, testing, and new insights in cytology. This accessible guide to diagnostic investigation and screening is ideal for daily laboratory use, taking a systematic approach to helping you understand major diagnostic criteria as well as the pitfalls and limitations of cytology. Quickly find the answers you need through a consistent chapter structure.Realize every possibility. Appropriate histopathological correlations and a consideration of the possible differential diagnosis accompany the cytological findings.
  • Principles of model building : an experimentation-aided approach to development of models for signaling networks / Ambhighainath Ganesan and Andre Levchenko -- Integrated inference and analysis of regulatory networks from multi-level measurements / Christopher S. Poultney, Alex Greenfield, and Richard Bonneau -- Swimming upstream : identifying proteomic signals that drive transcriptional changes using the interactome and multiple "-omics" datasets / Shao-shan Carol Huang and Ernest Fraenkel -- A framework for modeling the relationship between cellular steady-state and stimulus-responsiveness / Paul M. Loriaux and Alexander Hoffmann -- Stochastic modeling of cellular networks / Jacob Stewart-Ornstein and Hana El-Samad -- Quantifying traction stresses in adherent cells / Casey M. Kraning-Rush ... [et al.] -- CellOrganizer : image-derived models of subcellular organization and protein distribution / Robert F. Murphy -- Spatial modeling of cell signaling networks / Ann E. Cowan ... [et al.] -- Stochastic models of cell protrusion arising from spatiotemporal signaling and adhesion dynamics / Erik S. Welf and Jason M. Haugh -- Nonparametric variable selection and modeling for spatial and temporal regulatory networks / Anil Aswani ... [et al.] -- Quantitative models of the mechanisms that control genome-wide patterns of animal transcription factor binding / Tommy Kaplan and Mark D. Biggin -- Computational analysis of live cell images of the Arabidopsis thaliana plant / Alexandre Cunha ... [et al.] -- Multi-scale modeling of tissues using CompuCell3D / Maciej H. Swat ... [et al.] -- Multiscale model of fibrin accumulation on the blood clot surface and platelet dynamics / Zhiliang Xu ... [et al.].
  • Communication in the immune system by connexin channels / E. Oviedo-Orta and W. Howard Evans -- Gap junctions and connexins in the hematopoietic-immune system : structural considerations / T. Krenacs, I. Zsakovics and M. Rosendaal -- Approaches for studying the role(s) of gap junctions in the immune system / A.M. Glass, T.D. Nguyen, S. Taffet -- Gap junctions in antigen presenting cells / P.J. Saez, K.F. Shoji, J.C. Saez -- Connect the immune system : roles of gap junctions in antigen presentation and T cell activation / B. Pang, J. Neefjes -- Gap junctions and connexins in the immune defence against tumors / F. Salazar-Onfray -- Connexins in atherosclerosis / L. Burnier, B.R. Kwak -- Connexins in lung function and inflammation / M. Chanson, M. Koval -- The effect of oxidative stress on connexins in the vasculature / M. Billaud ... [et al.] -- The regulation of gap junctions and cellular coupling within the microvasculature in response to acute inflammation / D. Lidington, K. Tyml -- Impact of microglial activation on astroglial connexin expression and function in brain inflammation / C. Giaume ... [et al.] -- A role for connexins in inflammatory disorders of the epidermis / S. Donnelly, P. Martin -- Translating basic research on Cx43 gap junctions into therapies for reducing scarring and cardiac arrhythmia / J.M. Rhett ... [et al.] -- Connexin-based therapeutic approaches to inflammation in the central nervous system / J. Zhang ... [et al.] -- Enhancing epithelial tissue repair and reducing inflammation by targeting connexins / D.L. Becker ... [et al.].
  • This new volume of Methods in Cell Biology looks at methods for analyzing correlative light and electron microscopy (CLEM). With CLEM, people try to combine the advantages of both worlds, i.e. the dynamics information obtained by light microscopy and the ultrastructure as provided by electron microscopy. This volume contains the latest techniques on correlative microscopy showing that combining two imaging modalities provides more than each technique alone. Most importantly it includes the essential protocols, including tips, tricks and images for you to repeat these exciting techniques in your own lab. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.
  • This book reviews recent progress in cortical development research, focusing on the mechanisms of neural stem cell regulation, neuronal diversity and connectivity formation, and neocortical organization. Development of the cerebral cortex, the center for higher brain functions such as cognition, memory, and decision making, is one of the major targets of current research. The cerebral cortex is divided into many areas, including motor, sensory, and visual cortices, each of which consists of six layers containing a variety of neurons with different activities and connections. As this book explains, such diversity in neuronal types and connections is generated at various levels. First, neural stem cells change their competency over time, giving sequential rise to distinct types of neurons and glial cells: initially deep layer neurons, then superficial layer neurons, and lastly astrocytes. The activities and connections of neurons are further modulated via interactions with other brain regions, such as the thalamocortical circuit, and via input from the environment. This book on cortical development is essential reading for students, postdocs, and neurobiologists.
  • ch. 1. Cell Culture -- ch. 2. Preparation and Isolation of Cells -- ch. 3. Subcellular Fractionation and Isolation of Organelles -- ch. 4. Microscopy -- ch. 5. Characterization of Cellular Proteins -- ch. 6. Electrophoresis and Immunoblotting -- ch. 7. Protein Labeling and Immunoprecipitation -- ch. 8. Cell Cycle Analysis -- ch. 9. Cell Adhesion -- ch. 10. Extracellular Matrix -- ch. 11. In Vitro Reconstitution -- ch. 12. Cell Motility -- ch. 13. Organelle Motility -- ch. 14. Signal Transduction: Protein Phosphorylation -- ch. 15. Protein Trafficking -- ch. 16. Antibodies as Cell Biological Tools -- ch. 17. Macromolecular Interactions in Cells -- ch. 18. Cellular Aging and Death -- ch. 19. Whole Organism and Tissue Analysis -- ch. 20. Expression and Introduction of Macromolecules into Cells -- ch. 21. Fluorescent Protein Technology -- ch. 22. Cell Biology of Chromosomes and Nuclei -- ch. 23. Stem Cells -- ch. 24. Lipids -- ch. 25. Nanotechnology -- ch. 26. Viruses -- ch. 27. RNA-Based Methods in Cell Biology -- Appendix 1. Useful Information and Data -- Appendix 2. Laboratory Stock Solutions and Equipment -- Appendix 3. Commonly Used Techniques -- Appendix. Suppliers.
  • Consists of a core publication dated Apr. 1997, and updated by supplements 1 (July 1997)-<57 (July 2010)>. Content is peer-reviewed protocols for flow and image cytometry updated quarterly.
  • Includes methods for isolating, maintaining, and differentiating embryonic and adult stem cells.
  • The Interleukin-1 Family / Charles A. Dinarello and Mihai G. Netea -- IL-6 and Inflammatory Diseases / Daisuke Kamimura, Yasunobu Arima, Toshio Hirano, Hideki Ogura, and Masaaki Murakami -- The Roles of IL-17A and IL-17F in Infection and Inflammatory Disorders / Harumichi Ishigame and Susumu Nakae -- IL-18 / Wen Li, Yoshimasa Tanaka, and Haruki Okamura -- Interleukin-21: a Pleiotropic Mediator of Immunity and Inflammation with Broad Therapeutic Potential / Rosanne Spolski and Warren J. Leonard -- Interleukin-22: A Bridge Between Epithelial Innate Host Defense and Immune Cells / Xiaoting Wang and Wenjun Ouyang -- IL-23 in Health and Disease / Jonathan P. Sherlock, Luis A. Zuniga, and Daniel J. Cua -- IL-36: An Epithelial Cytokine Important in Psoriasis / John E. Sims, Solenne Vigne, Cem Gabay, and Jennifer E. Towne -- Anti-TNF Therapy: 20 Years from Our First Therapeutic Adventure / Jagdeep Nanchahal, Peter Taylor, Richard Williams, and Marc Feldmann -- Interleukin-25: Key Initiator of Type 2 Immune Responses / Hiroshi Nakajima, Tomohiro Tamachi, and Koichi Hirose -- Interleukin-33: Multifunctional Alarmin that Promotes Both Health and Disease / Kazufumi Matsushita and Tomohiro Yoshimoto -- Thymic Stromal Lymphopoietin (TSLP) / Steven F. Ziegler, Florence Roan, Bryan D. Bell, Thomas A. Stoklasek, Masayuki Kitajima, and Hongwei Han -- Interleukin-10: Cytokines in Anti-inflammation and Tolerence / Ashleigh Howes, Philippa Stimpson, Paul Redford, Leona Gabrysova, and Anne O'Garra -- Interleukin-27: Regulation of Immune Responses and Disease Development by a Pleiotropic Cytokine with Pro- and Anti-inflammatory Properties / Izuru Mizoguchi, Kaname Higuchi, Kana Mitobe, Ren Tsunoda, Junichiro Mizuguchi, and Takayuki Yoshimoto -- Interleukin-35: A Novel Mediator of Peripheral Tolerance / Greg M. Delgoffe and Dario A.A. Vignali.
  • Cytokines 2011
    1. General and cellular aspects -- 2. Life style factor and life events -- 3. Immunology and infections -- 4. Cancer -- 5. Cardiovascular and metabolic disease -- 6. Organs and tissue systems.
  • Fully updated, the 4th edition of Cytology helps you apply the latest diagnostic techniques for the interpretation of a complete range of cytological specimens. This medical reference book offers access to the newest methods and adjunct tests, all in a brief, highly readable format that makes quick work of reviewing the key information available on this powerful yet minimally invasive method. It's a perfectly practical bench manual for trainees and practicing pathologists. Take advantage of comprehensive yet concise coverage of nearly every type of cytology, including gynecologic, non-gynecologic, and FNA. Understand the clinical implications of your diagnoses and better equip yourself to make effective interpretations with clinical correlations emphasized throughout. Make the most informed reporting decisions through an in-depth analysis of common diagnostic pitfalls. Quickly find the answers you need with succinct, bulleted text, clinical pearls, and summaries of key points. Gain real-life perspective on a complete range of cytologic findings with over 700 full-color illustrations.
  • Also available: Print – 2014
    In recent decades, cytopathology has assumed an increasing role in the primary diagnosis of mass lesions owing to its ability to provide rapid, non-invasive, and timely information. With cytopathology at the forefront of diagnosis and prognostication of neoplastic lesions, it is essential that physicians involved in the diagnosis and treatment of precancerous lesions and cancer have a clear understanding of specimen collection techniques, terminology for reporting, and outcomes in various diagnostic categories. This book provides a comprehensive overview of the role of cytology at various body sites. The diagnostic details covered are abbreviated in comparison with those in pathology texts. Instead, a more clinical approach is taken, with the focus on the advantages and limitations of techniques and the key features of entities that are important to clinicians. Pathological clinical correlation is highlighted throughout the book, ensuring that it will be highly relevant for clinicians. In particular, physicians who deal with oncology patients will find it to be a rich source of guidance on how to use and understand cytopathology in the diagnosis and exclusion of malignancy.
  • This volume fulfills the need for an easy-to-use and authoritative synopsis of intra-abdominal organ cytopathology. Part of the Essentials in Cytopathology book series, the book is heavily illustrated with a full color art program, while the text follows a user-friendly outline format. The book also fits into the lab coat pocket and is ideal for portability and quick reference. Written by authorities in the field, Cytopathology of the Liver, Biliary Tract, Kidney and Adrenal Glands is a concise and valuable resource to pathologists on the subject of intra-abdominal organ cytopathology.
  • & Quot;Soft Tissue Cytopathology" by Anil V. Parwani, MD, PhD and Walid E. Khalbuss, MD, PhD, will fulfill the need for an easy-to-use and authoritative synopsis of soft tissue cytopathology. This volume, part of "The Essentials in Cytopathology book series," fits into the lab coat pocket and is ideal for portability and quick reference. Each volume in the series is heavily illustrated with a full color art program, while the text follows a user-friendly outline format. Anil V. Parwani, MD, PhD, is Associate Professor of Pathology at the University of Pittsburgh Medical Center - S.
  • Cytopreparation: Principles & Practice by Gary W. Gill fills a long-standing need for an easy-to-use and authoritative manual on the fundamentals of cytopreparation up-to-and- including microscopy, screening, and data analysis. The text describes in phenomenological terms the most common materials and methods of specimen collection through mounting for gyn, non-gyn, and FNA specimens, as well as the underlying mechanistic bases. The author provides his expertise and information that will empower and enable readers to review and improve their laboratories' cytopreparatory techniques as they apply to the vast majority of specimens. This unique volume provides facts that are not readily available anywhere. Cytopreparation: Principles & Practice is intended for everyone associated with, and involved in, making cytologic preparations that are useful for their intended purpose. It will serve as a valuable reference tool for educators in cytology and histology, cytotechnology and histotechnology students, cytotechnologists, cytopreparatory technicians, cytopathologists, anatomical/clinical pathologists, pathology residents and cytopathology fellows.
  • Part 1. Basics of the Cytoskeleton -- ACTIN / Vera Dugina, Richard Arnoldi, Paul A. Janmey and Christine Chaponnier -- Microtubules / Pavel Dráber and Eduarda Dráberová -- The Kinesin Superfamily / Linda Wordeman -- Basics of the Cytoskeleton: Myosins / Omar A. Quintero, Judy E. Moore and Christopher M. Yengo -- Part 2. Actin and Disease -- The Actin Cytoskeleton and Membrane Organisation in T Lymphocytes / Rhea Cornely, Thomas Grewal and Katharina Gaus -- Thin Filament Diseases of Striated Muscle / Anthony J. Kee and Edna C. Hardeman -- Filamins and Disease / Stephen P. Robertson and Philip B. Daniel -- LIM Kinase and Cancer Metastasis / Alice Schofield and Ora Bernard -- Actin Mutations and Deafness / Matías Morín, Fernando Mayo, Felipe Moreno and Miguel A. Moreno-Pelayo -- Therapeutic Targeting of the Actin Cytoskeleton in Cancer / Teresa Bonello, Jason Coombes, Galina Schevzov, Peter Gunning and Justine Stehn -- Part 3. Microtubules and Disease -- Microtubules as a Target in Cancer Therapy / April L. Risinger and Susan L. Mooberry -- Microtubules, Drug Resistance, and Tumorigenesis / Joshua A. McCarroll and Maria Kavallaris -- Posttranslational Modifications of Tubulin / Suzan K. Chao, Chia-Ping H. Yang and Susan Band Horwitz -- Stathmin and Cancer / Dominic Chi Hiung Ng and Frances Byrne -- The Biology and Pathobiology of Tau Protein / Garth F. Hall -- Tubulin-Related Malformations of Cortical Development / Xavier H. Jaglin, Jamel Chelly and Nadia Bahi-Buisson -- Part 4. Intermediate Filaments and Disease -- Spectrins in Human Diseases / Marie-Christine Lecomte -- Laminopathies / Nadir M. Maraldi and Giovanna Lattanzi -- Desmin and Heart Disease / J. Scott Pattison and Jeffrey Robbins -- Neurodegenerative Diseases and Intermediate Filaments / Rodolphe Perrot and Jean-Pierre Julien.
  • Central axonal development and pathology in early life / Robin L. Haynes and Hannah C. Kinney -- Microtubules in the nervous system / Nobuyuki Fukushima -- Tau phosphorylation / Jesús Avila and Félix Hernández -- Tau pathology / Nicolas Sergeant and Luc Buée -- Tauopathy and brain aging / Akihiko Takashima -- Microtubule-associated protein 4 / Kiyotaka Tokuraku ... [et al.] -- Structure of neural intermediate filaments / David A.D. Parry -- Alpha-internexin: the fourth subunit of neurofilaments in the mature CNS / Aidong Yuan and Ralph A. Nixon -- Peripherin pathology / Jesse R. McLean and Janice Robertson -- Neurofilament cross-bridge: a structure associated specifically with the neurofilament among the intermediate filament family / Takahiro Gotow -- Neurofilament transport / Andrew J. Grierson and Christopher C.J. Miller -- Knockout models of neurofilament proteins / Rodolphe Perrot and Jean-Pierre Julien -- Neurofilaments and radial growth: deconstruction of a hypothesis through the construction of gene targeted mice / Michael L. Garcia and Devin M. Barry -- Deregulation of cytoskeletal protein phosphorylation and neurodegeneration / Jyotshnabala Kanungo ... [et al.] -- Neurofilaments in aged animals / Shin-ichi Hisanaga, Takahiro Sasaki, and Atsuko Uchida -- Neurofilament changes in multiple sclerosis / Elizabeth Gray and Alastair Wilkins -- Intermediate filament interactions in neurons / Kevin G. Young and Rashmi Kothary -- Tropomyosins in neuronal morphogenesis and development / Nikki Margarita Curthoys, Peter William Gunning, and Thomas Fath -- The driving machinery for growth cone navigation / Takuro Tojima and Hiroyuki Kamiguchi -- Glial fibrillary acidic protein: the intermediate filament protein of astrocytes / Douglas L. Eng and Lawrence F. Eng -- Axonal transport mechanisms in cytoskeleton formation and regulation / Aidong Yuan and Ralph A. Nixon -- Axonal transport and motor neuron disease / Anna-Lena Ström ... [et al.] -- In vivo imaging of axonal transport in aging and Alzheimer's disease / Donna J. Cross and Satoshi Minoshima -- Regulation of cytoskeletal composition in neurons: transcriptional and post-transcriptional control in development, regeneration, and disease / Ben G. Szaro and Michael J. Strong -- Crosstalks between myelinating cells and the axonal cytoskeleton / Rodolphe Perrot and Joël Eyer -- Topographic regulation of neuronal intermediate filament proteins by phosphorylation: in health and disease / Parvathi Rudrabhatla and Harish C. Pant -- Cytoskeleton, axonal transport, and the mechanisms of axonal neuropathy / Hsinlin T. Cheng ... [et al.] -- Effects of insulin on tau and neurofilament / R. Schechter and K.E. Miller -- Ethanol effects on the cytoskeleton of nerve tissue cells / Sergio G. Evrard and Alicia Brusco.
  • Also available: Print – 2009
    The role of TNF in cancer / Harald Wajant -- Many checkpoints on the road to cell death: regulation of Fas-FasL interactions and Fas signaling in peripheral immune responses / Madhu Ramaswamy ... [et al.] -- FasL expression and reverse signaling / M. Lettau ... [et al.] -- Impact of TNF-R1 and CD95 internalization on apoptotic and antiapoptotic signaling / Stefan Schütze and Wulf Schneider-Brachert -- Ubiquitination and TNFR1 signaling / Ken-ichi Fujita and Srinivasa M. Srinivasula -- From biochemical principles of apoptosis induction by TRAIL to application in tumour therapy / Stefanie M. Cordier, Kerstin Papenfuss, and Henning Walczak -- Therapeutic targeting of TWEAK/Fn14 in cancer: exploiting the intrinsic tumor cell killing capacity of the pathway / Jennifer S. Michaelson and Linda C. Burkly -- APRIL in B-cell malignancies and autoimmunity / Fiona C. Kimberly, Jan Paul Medema, and Michael Hahne -- Autophagy in cancer and chemotherapy / Shida Yousefi and Hans-Uwe Simon -- Glucocorticoid-mediated apoptosis resistance of solid tumors / Ingrid Herr, Markus W. Büchler, and Jürgen Mattern -- Targeting death-receptors in radiation therapy / Olivier Niemöller and Claus Belka -- Death ligands designed to kill: development and application of targeted cancer therapeutics based on proapoptotic TNF family ligands / Jeannette Gerspach, Harald Wajant, and Klaus Pfizenmaier.
  • Dental Evolution -- Tooth development -- Dental Pulp is a Connective Tissue -- Dental Pulp Stem Cells (DPSC) -- Isolation methods of DPSC -- Characterization of DPSC -- Reprogramming of DPSC to induced pluripotent stem cells (iPSC) -- Immunomodulatory effects of DPSC -- Dental Pulp is a Complex Adaptive System.
  • Fluorescence microscopy is one of the most widely used tools in cell biology due its intrinsically high detection sensitivity coupled with the ability to genetically label proteins and other cellular structures with fluorescent tags. However, the resolution of fluorescence microscopy has historically been limited to about 200 nm laterally and 800 nm axially because of the diffraction limit of visible light. In the past five years, imaging below the diffraction limit ("super-resolution imaging") by localizing single fluorophores, one at a time (1-3), has opened a wide a variety of new biological systems for study. This Dissertation is a collection of both techniques for two and three dimensional super-resolution imaging as well as applications in bacterial and yeast imaging. References 1. Betzig E, et al (2006) Imaging intracellular fluorescent proteins at nanometer resolution. Science 313: 1642-1645. 2. Hess ST, Girirajan TPK & Mason MD (2006) Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys J 91: 4258-4272. 3. Rust MJ, Bates M & Zhuang X (2006) Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3: 793-795.
  • Diagnostic Cytopathology Board Review and Self-Assessment provides a comprehensive systems-based review of non-gynecological cytology including cytomorphology, pitfalls and ancillary studies, presented in a high-yield format with board-type multiple choice questions and detailed answers. It provides an excellent review, resource, and self-assessment for pathologists, cytopathologists, and cytotechnologists, as well as trainees (pathology residents, cytopathology fellows, and cytotechology students) who are preparing for board examinations or in-service examinations, in addition to those who are looking to fine-tune their cytology diagnostic skills. The volume is organized in a systems-based format including chapters covering lung, lymph nodes, pancreas, liver, gastrointestinal tract, thyroid, salivary gland, central nervous system, soft tissue and bone, kidney, adrenal, retroperitoneal, and exfoliative cytology of urine, serous fluids, and CSF. This book is enriched with high yield review, board exam-type questions, and high quality full-color images to maximize studying and review of cytopathology.
  • Introduction -- Female genital tract -- Respiratory -- Serous effusions -- Urine cytology -- Thyroid gland -- Haemopoietic -- Breast -- Salivary gland -- Liver, biliary tree and pancreas -- Childhood tumours -- Miscellaneous -- Techniques -- Self-assessment questions.
  • Cytopathology: the history, the present and the future direction -- Respiratory system -- Respiratory tract -- Serous cavities -- Serous effusions -- Breast -- Alimentary system -- Salivary glands -- Oral cavity -- Oesophagus and gastrointestinal tract -- Hepatobiliary system and pancreas -- Liver -- Gall bladder and extrahepatic bile ducts -- Pancreas -- Kidney and urinary tract -- Kidney and retroperitoneal tissues -- Urine cytology -- Lymphoreticular system -- Lymph nodes -- Other lymphoreticular organs -- Transplantation and immunosuppression -- Organ transplantation -- Immunosuppression -- Endocrine system -- Thyroid gland -- Other endocrine organs -- Male genital tract -- Prostate gland -- Testis and scrotum: cytology of testicular and scrotal masses and male infertility -- Female genital tract -- Vulva, vagina and cervix: normal cytology, hormonal and inflammatory conditions -- Cervical screening programmes -- Cervical intraepithelial neoplasia and squamous cell carcinoma of the cervix -- Glandular neoplasms of the cervix -- Other tumours and lesions of cervix, vulva and vagina -- Cytology of the body of the uterus -- Ovaries, fallopian tubes and associated lesions -- Skin, soft tissues and musculoskeletal system -- Skin -- Soft tissue and musculoskeletal system -- Synovial fluid -- Central nervous system -- Brain and cerebrospinal fluid -- Eyelids, orbit and eye -- Childhood tumours -- New techniques.
  • Following their own brilliant careers in haeme protein research, the outstanding scientists Jonathan and Beatrice Wittenberg continue to provide inspiration to the research community in the study of oxygen-binding proteins. Their research has provided the intellectual stimulus to bring together scientists from all over the world with the common goal of developing fascinating new ideas and performing innovative experiments. This book is dedicated to Jonathana (TM)s and Beaa (TM)s lifetime careers. It further illuminates the facts and ideas which dot the paths they traced in Biochemistry and Physiology, elaborating on how these landmark achievements were made and how the haeme proteins community still refers to them. With the field of haeme protein science such a flourishing area, the contributors to this book predict Jonathan and Bea, having played such a seminal role, will continue to be key figures for quite some time to come.
  • Also available: Print – 2005
    The secretory capacity of a cell depends on the efficiency of endoplasmic reticulum-associated degradation / M. Molinari and R. Sitia -- Recognition and delivery of ERAD substrates to the proteasome and alternative paths for cell survival / A.A. McCracken and J.L. Brodsky -- CPY* and the power of yeast genetics in the elucidation of quality control and associated protein degradation of the endoplasmic reticulum / D.H. Wolf and A. Schäfer -- The role of the ubiquination machinery in dislocation and degradation of endoplasmic reticulum proteins / M. Kikkert, G. Hassink, and E. Wiertz -- The role of p97/Cdc48p in endoplasmic reticulum-associated degradation : from the immune system to yeast / S. Bar-Nun -- The ins and outs of intracellular peptides and antigen presentation by MHC Class I molecules / T. Groothuis and J. Neefjes -- Entry of protein toxins into mammalian cells by crossing the endoplasmic reticulum membrane : co-opting basic mechanisms of endoplasmic reticulum-associated degradation / J.M. Lord , L.M. Roberts, and W.I. Lencer.
  • Also available: Print – 2010
    The HIF-2[alpha]-driven pseudo-hypoxic phenotype in tumor aggressiveness, differentiation, and vascularization / A. Pietras, A.S. Johnsson and S. Pahlman -- Hypoxia and hypoxia inducible factors in cancer stem cell maintenance / Z. Li and J.N. Rich -- Role of carcinoma-associated fibroblasts and hypoxia in tumor progression / A.J. Giaccia and E. Schipani -- The role of hypoxia regulated microRNAs in cancer / R. McCormick ... [et al.] -- Oxygen sensing: A common crossroad in cancer and neurodegeneration / A. Quaegebeur and P. Carmeliet -- Hypoxia-inducible factors as essential regulators of inflammation / H.Z. Imtiyaz and M.C. Simon -- Hypoxia and metastasis in breast cancer / H. Rundqvist and R.S. Johnson.
  • The existence of 'cancer stem cells' (CSCs) has been a topic of heated debate for the last few years within the field of cancer biology. Their continuous characterization in a variety of solid tumors has lead to an abundance of evidence supporting their existence. CSCs are believed to be responsible for resistance against conventional treatment regimes of chemotherapy and radiation, ultimately, leading to metastasis and patient demise. To help aid clinicians, pharmaceutical companies and academic labs investigating how to better kill these highly aggressive cells we have summarized the DNA repair mechanism(s) and their role in the maintenance and regulation of both normal and cancer stem cells. Our book represents a comprehensive investigation into the highly effective DNA repair mechanisms of CSCs and what we need to understand in order to develop more advanced therapies to eradicate them from patients. Currently, there are no other published works entirely on DNA repair and Cancer Stem Cells. In addition, our book provides a comprehensive overview of CSC isolation and characterization from a variety of solid tumor types.
  • In humans, male infertility is caused by a number of factors such as the external environment, nutrient changes, genotoxins, and mutagens. This book discusses the events that occur in the normal sperm head and govern the structure-function relationship from the time of spermatogenesis to that of fertilization or egg activation.
  • Research on dyneins has a direct impact on human diseases, such as viruses and cancer. With an accompanying website showing over one hundred streaming videos of cell dynamic behavior for best comprehension of material, Dynein: Structure, Biology and Disease is the only reference covering the structure, biology and application of dynein research to human disease. From bench to bedside, Dynein: Structure, Biology and Disease offers research on fundamental cellular processes to researchers and clinicians across developmental biology, cell biology, molecular biology, biophysics, biomedicine, genetics and medicine. . Broad-based up-to-date resource for the dynein class of molecular motors . Chapters written by world experts in their topics . Numerous well-illustrated figures and tables included to complement the text, imparting comprehensive information on dynein composition, interactions, and other fundamental features.
  • "ENOX Proteins" documents a unique family of cell surface proteins which are the exclusive discovery (subsequently confirmed by others) of the authors, their students and their colleagues. An ENOX-based mechanism is provided for how cells increase in size that is both unique and applicable not only to cancer and cancer therapy but also to production agriculture and biomass increase. The role of ENOX proteins in biological time-keeping is described along with evidence for oscillations in the ratios of electron spin pairs defining ortho and para water states that form highly coordinated populations of coherent water of interest to physicists as the underlying mechanism. The age-related NADH oxidases that appear around age 30 and increase steadily thereafter with potentially causal involvements in atherogenesis and skin aging have been identified as five members of the TM-9 protein superfamily of all with different chromosomal locations. The arNOX proteins initially are membrane anchored but functionally-active N-terminal fragments that are shed into body fluids. Except for critical functional motifs, all of the ENOX protein family members have distinct protein sequences and chromosomal origins. A fourth member of the ENOX protein family occurs in plants as the primary target for both natural and synthetic growth hormone (auxin)-stimulated rapid cell enlargement. Despite masquerading as intractable and difficult to clone and characterize, ENOX proteins offer remarkable opportunities for research, commercial development and outside confirmation of new paradigms to help explain complex biological processes.
  • Electric Cell-Substrate Impedance Sensing Concept to Commercialization / Ivar Giaever and Charles R. Keese -- Protein Kinase C Isoforms in the Formation of Focal Adhesion Complexes: Investigated by Cell Impedance / Havovi Chichger, Katie L. Grinnell and Elizabeth O. Harrington -- ECIS as a Tool in the Study of Metastasis Suppressor Genes: Epithelial Protein Lost In Neoplasm (EPLIN) / Andrew J. Sanders, Vladimir M. Saravolac, Malcolm D. Mason and Wen G. Jiang -- Electrical Cell-Substrate Impedance Sensing for Measuring Cellular Transformation, Migration, Invasion, and Anticancer Compound Screening / Bryan Plunger, Chang Kyoung Choi and Tim E. Sparer -- Epithelial-Mesenchymal Transition and the Use of ECIS / Jane Lane and Wen G. Jiang -- Cell Growth and Cell Death Studied by Electric Cell-Substrate Impedance Sensing / Judith Anthea Stolwijk, Stefanie Michaelis and Joachim Wegener -- Tight Junctions in Cancer Metastasis and Their Investigation Using ECIS (Electric Cell-Substrate Impedance Sensing) / Tracey A. Martin and Wen G. Jiang -- Epithelial Wound Healing and the Effects of Cytokines Investigated by ECIS: Electric Cell-Substrate Impedance Sensing in Epithelial Research / Katalin Szaszi, Matthew Vandermeer and Yasaman Amoozadeh -- Tumour-Endothelial and Tumour-Mesothelial Interactions Investigated by Impedance Sensing Based Cell Analyses / Wen G. Jiang, Lin Ye, Haiying Ren, Ann Kift-Morgan and Nicholas Topley, et al. -- Application of Electric Cell-Substrate Impedance Sensing in Evaluation of Traditional Medicine on the Cellular Functions of Gastric and Colorectal Cancer Cells / Lin Ye, Ke Ji, Jiafu Ji, Rachel Hargest and Wen G. Jiang -- Electric Cell-Substrate Impedance Sensing as a Screening Tool for Wound Healing Agents / Cheuk Lun Liu, Jacqueline Chor Wing Tam, Andrew J. Sanders, David G. Jiang and Chun Hay Ko, et al. -- ECIS, Cellular Adhesion and Migration in Keratinocytes / David C. Bosanquet, Keith G. Harding and Wen G. Jiang -- Current and Future Applications of ECIS Models to Study Bone Metastasis / Lin Ye, Sivan M. Bokobza, Howard G. Kynaston and Wen G. Jiang.
  • Chapter 1. Electron Microscopy of Viruses -- Chapter 2. Bacterial TEM : New Insights from Cryo-Microscopy -- Chapter 3. Analysis of the Ultrastructure of Archaea by Electron Microscopy -- Chapter 4. Chlamydomonas : Cryopreparation Methods for the 3-D Analysis of Cellular Organelles -- Chapter 5. Ultrastructure of the Asexual Blood Stages of Plasmodium falciparum -- Chapter 6. Electron Tomography and Immuno-labeling of Tetrahymena thermophila Basal Bodies -- Chapter 7. Electron Microscopy of Paramecium (Ciliata).
  • Immune privilege of stem cells / Naoki Ichiryu and Paul J. Fairchild -- Immunogenicity of in vitro maintained and matured populations : potential barriers to engraftment of human pluripotent stem cell derivatives / Chad Tang, Irving L. Weissman, and Micha Drukker -- Hematopoietic and nature killer cell development from human pluripotent stem cells / Zhenya Ni, David A. Knorr, and Dan S. Kaufman -- Evaluation of immunogenicity of rat ES-cell derived endothelial cells / Martina Seifert and Juliane Ladhoff -- Interaction of ES cell derived neural progenitor cells with natural killer cells and cytotoxic T cells / Casimir de Rham and Jean Villard -- Strategies to generate induced pluripotent stem cells / Michael Hayes and Nicholas Zavazava -- Differentiation and lineage commitment of murine embryonic stem cells into insulin producing cells / Sudhanshu P. Raikwar and Nicholas Zavazava -- Mouse ES cell-derived hematopoietic progenitor cells / Eun-Mi Kim, Gohar Manzar, and Nicholas Zavazava -- Directed differentiation of embryonic stem cells to the T-lymphocyte lineage / Haydn C.-Y. Liang, Roxanne Holmes, and Juan Carlos Zúñiga-Pflücker -- Development of hematopoietic stem and progenitor cells from mouse embryonic stem cells, in vitro, supported by ectopic human HOXB4 expression / Sandra Pilat, Sebastian Carotta, and Hannes Klump -- Histone modification profiling in normal and transformed human embryonic stem cells using micro chromatin immunoprecipitation, scalable to genome-wide microarray analyses / Angelique Schnerch, Shravanti Rampalii, and Mickie Bhatia -- Combined total proteomic and phosphoproteomic analysis of human pluripotent stem cells / Junjie Hou ... [et al.] -- Transcriptional regulatory mechanisms that govern embryonic stem cell fate / Satyabrata Das and Dana Levasseur.
  • Embryonic stem cell protocols. 2nd ed. v. 1-2, 2006
    Springer Protocols
    v. 2, 2006 Springer Protocols
    v. 1. Isolation and characterization -- v. 2. Differentiation models.
  • Embryonic stem cells 2006
    Also available: Print – 2006
    Derivation and maintenance of embryonic stem cells -- Differentiation of embryonic stem cells.
  • Defined culture media for human embryonic stem cells / T. Ludwig and J. Thomson -- Generation of disease-specific human embryonic stem cell lines / S. Minger -- Characterization and differentiation of human embryonic stem cells / A. Laslett ... [et al.] -- Genetic modification of human embryonic stem cells / T. Zwaka -- Hematopoietic differentiation / C. Cerdan ... [et al.] -- Neural differentiation / Z.-J. Zhang ... [et al.] -- Germ cell differentiation / V. Angeles and R. Pera -- Mesodermal differentiation / N. Sharon and N. Benvenisty -- Three-dimensional culture of human embryonic stem cells / S. Gerecht ... [et al.] -- Extraembryonic cell differentiation / L. Armstrong and M. Lako -- Pancreatic cell differentiation / B. Fishman ... [et al.] -- Cardiomyocyte differentiation / D. Singla ... [et al.] -- Human embryonal carcinoma (EC) cells: Complementary tools for embryonic stem cell research / P. Tonge and P. Andrews -- Quality control of human stem cell lines / G. Stacey.
  • In biochemistry, a metalloprotein is a generic term for a protein that contains a metal cofactor. The metal may be an isolated ion or may be coordinated with a nonprotein organic compound, such as the porphyrin found in hemoproteins. In some cases, the metal is co-coordinated with a side chain of the protein and an inorganic nonmetallic ion. This kind of protein-metal-nonmetal structure is seen in iron-sulfur clusters Metalloproteins deals with all aspects related to the intracellular and extracellular metal-binding proteins, including their structures, properties and functions. The biological roles of metal cations and metal-binding proteins are endless. They are involved in all crucial cellular activities. Many pathological conditions are related to the problematic metal metabolism. Research in metalloprotein-related topics is therefore rapidly growing, and different aspects of metal-binding proteins progressively enter curricula at Universities and even at the High School level on occasion. However, no key resource providing basic, but comprehensible knowledge on this rapidly expanding field exists. The Encyclopedia of Metalloproteins aims to bridge this gap, and will attempt to cover various aspects of metalloprotein/metalloproteomics and will deal with the different issues related to the intracellular and extracellular metal-binding proteins, including their structures, properties and functions. The goal is to cover exhaustively all catalytically and biologically crucial metal ions and to find at least one interacting protein for other metal ions. The Encyclopedia of Metalloproteins will provide a key resource for advanced undergraduate and graduate students, researchers, instructors, and professors interested in protein science, biochemistry, cell biology, and genetics.
  • The Endoplasmic Reticulum (ER) is an organelle with extraordinary signaling and homeostatic functions. It is the organelle responsible for protein folding, maturation, quality control and trafficking of proteins destined for the plasma membrane or for secretion into the extracellular environment. Failure, overloading or malfunctioning of any of the signaling or quality control mechanisms occurring in the ER may provoke a stress condition known as âER stressâ. Accumulating evidence indicates that ER stress may dramatically perturb interactions between the cell and its environment, and contribute to the development of human diseases, ranging from metabolic diseases and cancer to neurodegenerative diseases, or impact therapeutic outcome. This book primarily focuses on the pathophysiology of ER stress. It introduces the molecular bases of ER stress, the emerging relevance of the ER-mitochondria cross-talk, the signaling pathways engaged and cellular responses to ER stress, including the adaptive Unfolded Protein Response (UPR), autophagy as well as cell death. Next the book addresses the role of ER stress in physiology and in the etiology of relevant pathological conditions, like carcinogenesis and inflammation, neurodegeneration and metabolic disease. The last chapter describes how ER stress pathways can be targeted for therapeutic benefit. Altogether, this book will provide the reader with an exhaustive view of ER stress biology and the latest insights in the role of ER stress in relevant human diseases.
  • pt. B ScienceDirect
  • The endothelial cytoskeleton : multifunctional role of the endothelial actomyosin cytoskeleton / Yuka Shimizu and Joe G.N. Garcia -- Emerging themes on pulmonary microvascular endothelial cell : microtubules and microtubule associated proteins / Cristiaan D. Ochoa, Ron Balczon, and Troy Stevens -- Endothelial actin cytoskeleton and angiogenesis / Sadiqa K. Quadri -- Adherens junctions and endothelial cytoskeleton / Younes Smani and Tarik Smani-Hajami -- Mechanical force transmission via the cytoskeleton in vascular endothelial cells / Bori Mazzag ... [et al.] -- The functional role of the microtubule/microfilament cytoskeleton in the regulation of pulmonary vascular endothelial barrier / Irina B. Alieva and Alexander D. Verin -- Membrane-cytoskeleton interactions and control of vesicle trafficking in endothelial cells / Felicia Antohe -- Cell-matrix adhesion proteins in the regulation of endothelial permeability / Jurjan Aman, Geerten P. van Nieuw Amerongen, and Victor W.M. van Hinsbergh -- Endothelial cells and the regulation of platelet function / P.C. Redondo ... [et al.].
  • Epithelial cell culture protocols 2002
    Springer Protocols
  • Erythropoiesis: an overview / B.E. Torbett and J.S. Friedman -- Regulation of endogenous erythropoietin production / D.R. Mole and P.J. Ratcliffe -- Biology of erythropoietin / G. Molineux and A.M. Sinclair -- Erythropoiesis - genetic abnormalities / J.T. Prchal and X.T. Gregg -- Studies of erythropoiesis and the discovery and cloning of recombinant human erythropoietin / M.A. Foote -- Commercial production of recombinant erythropoietins / A.S. Chuck ... [et al.] -- Biosimilar epoetins / H. Schellekens -- New molecules and formulations / S.G. Elliott -- Structural basis for the signal transduction of erythropoietin / T.D. Osslund -- Intracellular signaling by the erythropoietin receptor / H.F. Lodish ... [et al.] -- Mechanism of erythropoietin receptor activation / S.N. Constantinescu -- Pharmacokinetics of erythropoiesis-stimulating agents / S. Doshi ... [et al.] -- Use of erythropoietic stimulating agents in the setting of renal disease / D.A. Goodkin -- Abuse of recombinant erythropoietins and blood products by athletes / D.H. Catlin and C.K. Hatton -- Role and regulation of iron metabolism in erythropoiesis and disease / T.L. Arvedson and B.J. Sasu -- Nonhematopoietic effects of erythropoiesis-stimulating agents / W. Jelkmann, R. Depping and E. Metzen.
  • Basic Research/Mechanisms -- A Historical Overview and Concepts of Mesenchymal Stem Cells / Shihua Wang, Robert Chunhua Zhao -- Biology of MSCs Isolated from Different Tissues / Simone Pacini -- Secretome of Mesenchymal Stem Cells / Yuan Xiao ... [et al.] -- Immunomodulatory Properties of Mesenchymal Stem Cells and Related Applications / Lianming Liao, Robert Chunhua Zhao -- Mesenchymal Stem Cell Homing to Injured Tissues / Yaojiong Wu, Robert Chunhua Zhao -- Major Signaling Pathways Regulating the Proliferation and Differentiation of Mesenchymal Stem Cells / Joseph D. Lamplot ... [et al.] -- MicroRNAs in Mesenchymal Stem Cells / Mohammad T. Elnakish, Ibrahim A. Alhaider, Mahmood Khan -- Genetic Modification of MSCs for Pharmacological Screening / Jie Qin, Martin Zenke -- Control of Mesenchymal Stem Cells with Biomaterials / Sandeep M. Nalluri, Michael J. Hill, Debanjan Sarkar -- Clinical Translation -- Mesenchymal Stem Cells for Cardiovascular Disease / Wei Wu, Shuyang Zhang -- Mesenchymal Stem Cells as Therapy for Graft Versus Host Disease: What Have We Learned? / Partow Kebriaei ... [et al.] -- Mesenchymal Stem Cells for Liver Disease / Feng-chun Zhang -- Mesenchymal Stem Cells for Bone Repair / Hongwei Ouyang, Xiaohui Zou, Boon Chin Heng, Weiliang Shen -- Mesenchymal Stem Cells for Diabetes and Related Complications / Vladislav Volarevic, Majlinda Lako, Miodrag Stojkovic -- Mesenchymal Stromal Cell (MSC) Therapy for Crohn's Disease / Jignesh Dalal -- The Summary of Stroke and Its Stem Cell Therapy / Renzhi Wang ... [et al.] -- Mesenchymal Stem Cell Transplantation for Systemic Lupus Erythematosus / Lingyun Sun -- International Regulations and Guidelines Governing Stem Cell Based Products -- Considerations of Quality Control Issues for the Mesenchymal Stem Cells-Based Medicinal Products / Bao-Zhu Yuan ... [et al.] -- Regulations/Ethical Guidelines on Human Adult/Mesenchymal Stem Cell Clinical Trial and Clinical Translation / Xiaomei Zhai, Renzong Qiu.
  • Introduction to stem cells -- Basic biology/mechanisms -- Tissue and organ development -- Methods -- Applications -- Regulation and ethics.
  • Pt. 1. Research biobanking : the traditional approach -- Consent to biobank research : one size fits all? / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- What no one knows cannot hurt you : the limits of informed consent in the emerging world of biobanking / Arthur L. Caplan -- Users and uses of the biopolitics of consent : a study of DNA banks / Pascal Ducournau and Anne Cambon-Thomsen -- Information rights on the edge of ignorance / Anne Maria Skrikerud -- The dubious uniqueness of genetic information / Anne Maria Skrikerud -- Duties and rights of biobank participants : principled autonomy, consent, voluntariness and privacy / Lars Øystein Ursin -- Biobanking and disclosure of research results : addressing the tension between professional boundaries and moral intuition / Lynn G. Dressler -- Biobanks and our common good / Erik Christensen -- Trust, distrust and co-production : the relationship between research biobanks and donors / Pascal Ducournau and Roger Strand -- Scientific citizenship, benefit, and protection in population-based research / Vilhj́almur Árnason -- Pt. 2. Research Biobanking : towards a new conceptual approach -- Mapping the language of research biobanking : an analogical approach / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- The use of analogical reasoning in umbilical cord blood biobanking / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- The Alexandria plan : creating libraries for human tissue research and therapeutic use / Laurie Zoloth -- The art of biocollections / Anne Hambro Alnæs -- The health dugnard : biobank participation as the solidary pursuit of the common good / Lars Øystein Ursin and Berge Solberg -- Embodied gifting : reflections on the role of information in biobank recruitment / Klaus Hoeyer -- Conscription to biobank research? / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- Ownership rights in research biobanks : do we need a new king of 'biological property'? / Paula Lobato de Faria -- Legal challenges and strategies in the regulation of research biobanking / Elisabeth Rynning -- Annexation of life : the biopolitics of industrial biology / Jan Reinert Karlsen and Roger Strand -- In the ruins of Babel : should biobank regulations be harmonized / Jan Reinert Karlsen, Jan Helge Solbak, and Roger Strand.
  • Also available: Print – 2013
    The evolution of single cells into multicellular organisms was mediated, in large part, by the extracellular matrix. The proteins and glycoconjugates that make up the extracellular matrix provide structural support to cellular complexes, facilitate cell adhesion and migration, and impart mechanical properties that are important for tissue function. Each class of ECM macromolecule has evolved to incorporate distinctive properties that are defined by conserved modules that are mixed together to achieve appropriate function. This volume provides a comprehensive analysis of how the major ECM components evolved over time in order to fill their specific roles found in modern organisms. The major focus is on the structural matrix proteins, matricellular proteins, and more complex ECM structures such as basement membranes. Adhesive proteins and their receptors are also discussed.The series Biology of Extracellular Matrix is published in collaboration with the American Society for Matrix Biology.
  • Exocytosis and endocytosis 2008
    Springer Protocols
    Vesicular trafficking : molecular tools and targets / Elena V. Vassilieva and Asma Nusrat -- Pharmacological inhibition of endocytic pathways : is it specific enough to be useful? / Andrei I. Ivanov -- In vitro assays to measure SNARE-mediated vesicle fusion / Susanne Kreye, Jörg Malsam, and Thomas H. Söllner -- Imaging Ca²⁺-triggered exocytosis of single secretory granules on plasma membrane lawns from neuroendocrine cells / Thorsten Lang -- FRAP analysis of secretory granule lipids and proteins in the sea urchin egg / Julian L. Wong and Gary M. Wessel -- A cell-free assay for endocytosis of E-cadherin / Toshiaki Sakisaka and Yoshimi Takai -- Cell-surface biotinylation to study endocytosis and recycling of occludin / Noriyuki Nishimura and Takuya Sasaki -- Fractionation of subcellular membrane vesicles of epithelial and nonepithelial cells by OptiPrep density gradient / Xuhang Li and Mark Donowitz -- Identification and characterization of interacting partners of Rab GTPases by yeast two-hybrid analyses / Mark Kail and Angelika Barnekow -- High-throughput analysis of the dynamics of recycling cell surface proteins / Roland Glovers, David E. James, and Adelle C.F. Coster -- Studying phagocytosis by live-cell scintillation proximity assay / Walter Stockinger and Axel Nohturfft -- Transcytosis of polymeric immunoglobin A in polarized Madin-Darby canine kidney cells / Asli Oztan, Christine Rondanino, and Gerard Apodaca -- The use of syntaxin chimeras to study polarized protein trafficking in epithelial cells / Martin B.A. ter Beest -- Targeting the epithelial SNARE machinery by bacterial neurotoxins / Véronique Proux-Gillardeaux and Thierry Galli -- Endocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor / Munekazu Yamakuchi ... [et al.] -- Molecular dissection of HCL secretion in gastric parietal cells using streptolysin O permeabilization / Xia Ding .. [et al.] -- Addressing membrane protein topology using the fluorescence protease protection (FPP) assay / Holger Lorenz, Dale W. Hailey, and Jennifer Lippincott-Schwartz -- Visualizing clathrin-mediated IgE receptor internalization by electron and atomic force microscopy / Alan R. Burns ... [et al.] -- Analyzing endosomes in nonsectioned cells by transmission electron microscopy / Willem Stoorvogel -- Imaging exocytosis of single insulin secretory granules with TIRF microscopy / Shinya Nagamatsu and Mica Ohara-Imaizumi -- Reconstitution of depolarization and Ca²⁺-evoked secretion in Xenopus oocytes monitored by membrane capacitance / Roy Cohen, Bernhard M. Schmitt, and Daphne Atlas -- Low-noise recording of single-vesicle capacitance steps in cell-attached patches / Vitaly Klyachko, Zhenjie Zhang, and Meyer Jackson -- Good practices in single-cell amperometry / David J. Machado, Mónica S. Montesinos, and Ricardo Borges -- Analysis of single-vesicle exocytotic events recorded by amperometry / Eugene V. Mosharov -- Application of RNAi technology and fluorescent protein markers to study membrane traffic in Caenorhabditis elegans / Dmitry Poteryaev and Anne Spang -- FM 1-43 labeling of synaptic vesicle pools at the Drosophila neuromuscular junction / Patrik Verstreken, Tomoko Ohyama, and Hugo J. Bellen -- Probing E-cadherin endocytosis by morpholino-mediated Rab5 knockdown in zebrafish / Florian Ulrich and Carl-Philipp Heisenberg -- Quantifying endocytosis in vivo using intravital two-photon microscopy / Ruben M. Sandoval and Bruce A. Molitoris.
  • Also available: Print – 2011
    An overview of extracellular matrix structure and function / Jürgen Engel and Matthias Chiquet -- Fibronectin and other adhesive glycoproteins / Jielin Xu and Deane Mosher -- Collagens, suprastructures, and collagen fibril assembly / David E. Birk and Peter Brückner -- Basement membranes / Jeffrey H. Miner -- Hyaluronan and the aggregating proteoglycans / Thomas N. Wight, Bryan P. Toole, and Vincent C. Hascall -- Small leucine-rich proteoglycans / Renato V. Iozzo ... [et al.] -- Microfibrils and fibrillin / Dirk Hubmacher and Dieter P. Reinhardt -- Elastin / Beth A. Kozel, Robert P. Mecham, and Joel Rosenbloom -- Lysyl oxidase and lysyl oxidase-like enzymes / Herbert M. Kagan and Faina Ryvkin -- The fibulins / Marion A. Cooley and W. Scott Argraves -- Matricellular proteins / David D. Roberts and Lester F. Lau.
  • Also available: Print – 2011
    Matrix metalloproteinases and their inhibitors in turnover and degradation of extracellular matrix / Sean E. Gill and William C. Parks -- Role of cysteine cathepsins in extracellular proteolysis / Dieter Brömme and Susan Wilson -- Cooperation between proteolysis and endocytosis in collagen turnover / Thomas H. Bugge and Niels Behrendt -- Pericellular proteolysis / Judith S. Bond, Timothy R. Keiffer, and Qi Sun -- Triple helicase activity and the structural basis of collagenolysis / Hideaki Nagase and Robert Visse -- Structural basis of extracellular matrix interactions with matrix metalloproteinases / Steven R. Van Doren -- The role of matrix metalloproteinases in cellular invasion and metastasis / Elena I. Deryugina and James P. Quigley -- Degradation of bone and the role of osteoclasts, bone lining cells and osteocytes / Vincent Everts and Paul Saftig -- Consequences of elastolysis / A. McGarry Houghton, Majd Mouded, and Steven D. Shapiro.
  • Also available: Print – 2013
    Cells in the developing embryo depend on signals from the extracellular environment to help guide their differentiation. An important mediator in this process is the extracellular matrix - secreted macromolecules that interact to form large protein networks outside the cell. During development, the extracellular matrix serves to separate adjacent cell groups, participates in establishing morphogenic gradients, and, through its ability to interact directly will cell-surface receptors, provides developmental clocks and positional information. This volume discusses how the extracellular matrix influences fundamental developmental processes and how model systems can be used to elucidate ECM function. The topics addressed range from how ECM influences early development as well as repair processes in the adult that recapitulate developmental pathways.The series Biology of Extracellular Matrix is published in collaboration with the American Society for Matrix Biology.
  • 1. Overview of extracellular vesicles in health and disease / Rienk Nieuwland, Edwin van der Pol, and Augueste Sturk -- 2. The biogenesis of exosomes / D. Michiel Pegtel -- 3. Extracellular vesicle-mediated epigenetic reprogramming of cells / Giovanni Camussi, Federica Collino, and Maria C. Deregibus -- 4. An overview of novel and conventional methods to detect extracellular vesicles / Edwin van der Pol, Ton G. van Leeuwen, and Rienk Nieuwland -- 5. Preanalytical variables / Romaric Lacroix, Coralie Judicone, Stéphane Robert, Laurent Arnaud, Florence Sabatier, and Françoise Dignat-George -- 6. Procoagulant assays / Barry J. Woodhams -- 7. Capture-based assays for extracellular vesicles within the blood / Jean Amiral -- 8. Flow cytometry / Romaric Lacroix, Stephane Robert, Florence Sabatier, and Francoise Dignat-George -- 9. Atomic force microscopy measurement of extracellular vesicles derived from plasma / Yuana Yuana and Susanne Osanto -- 10. Light-scattering methods to characterize extracellular vesicles / Don A. Gabriel, Sam Glover, Regina Bowling, and Nigel Key -- 11. Nanoparticle tracking analysis / Chris Gardiner and Rebecca Dragovic -- 12. Extracellular vesicle proteomic analysis / Andrés F. Parguiña and Ángel García -- 13. Microvesicles and exosomes in cancer / Rebecca Karp and Jeffrey Zwicker -- 14. Extracellular vesicles and tissue factor / Pierre-Emmanuel Rautou and Nigel Mackman -- 15. Extracellular vesicles in normal pregnancy and pre-eclampsia / Ian L. Sargent, Rebecca A. Dragovic, Dionne S. Tannetta, and Christopher W. Redman -- 16. Extracellular vesicles in cardiovascular disease / Anne-Clémence Vion and Chantal M. Boulanger -- 17. Extracellular vesicles in immunology / Els J. van der Vlist, Esther N.M. Nolte-'t Hoen, and Marca H.M. Wauben.
  • Articular cartilage is a unique and highly specialized avascular connective tissue in which the availability of oxygen and glucose is significantly lower than synovial fluid and plasma. Glucose is an essential source of energy during embryonic growth and fetal development and is vital for mesenchymal cell differentiation, chondrogenesis and skeletal morphogenesis. Glucose is an important metabolic fuel for differentiated chondrocytes during post-natal development and in adult articular cartilage and is a common structural precursor for the synthesis of extracellular matrix glycosaminoglycans.
  • Introduction and historical perspective -- FNAC technique and slide preparation -- Diagnostic interpretation of FNAC material -- Diagnostic dilemmas in FNAC practice: cystic lesions -- Diagnostic dilemmas in FNAC practice: lymphoid infiltrates -- Diagnostic dilemmas in FNAC practice: metastatic tumours -- Diagnostic dilemmas in FNAC cytology: small round cell tumours -- Diagnostic dilemmas in FNAC cytology: soft-tissue lesions -- Diagnostic dilemmas in FNAC cytology: difficult breast lesions -- Principles of safe practice: the role of FNAC in clinical management -- Principles of safe FNAC practice: FNAC versus core biopsy -- Principles of safe FNAC practice: medicolegal issues.
  • Also available: Print – 2007
    Principles of flow cytometry / Marion G. Macey -- Cell preparation / Desmond A. McCarthy -- Fluorochromes and fluorescence / Desmond A. McCarthy -- Quality control in flow cytometry / David Barnett and John T. Reilly -- Experimental design, data analysis, and fluorescence quantitation / Mark Lowdell -- Apoptosis detection by flow cytometry / Paul Allen and Derek Davies -- DNA analysis by flow cytometry / Derek Davies and Paul Allen -- Immunological studies of human cells / Ulrika Johansson -- Calcium: cytoplasmic, mitochondrial, endoplasmic reticulum, and flux measurements / Gary Warnes and Marion G. Macey -- Further functional studies / Marion G. Macey -- Cell sorting by flow cytometry / Derek Davies.
  • This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.
  • Introduction to fluorescent proteins / Marc Zimmer -- Lessons learned from the structure of aequorea victoria fluorescent proteins / S. James Remington -- Optimization of fluorescent proteins / Mark A. Rizzo -- Development of new colors from coral fluorescent proteins / Nathan C. Shaner -- Red fluorescent proteins : multipurpose markers for live cell imaging / Jörg Wiedenmann and G. Ulrich Nienhaus -- Optical highlighter photophysical properties / George H. Patterson -- Far red and near infrared fluorescent proteins / Jun Chu, Yan Xing, and Michael Z. Lin -- Genetically-encoded fluorescent proteins and FRAP / James G. McNally -- Optical highlighters : applications to cell biology / Malte Renz and Jennifer Lippincott-Schwartz -- Optogenetic tools derived from plant photoreceptors / John M. Christie -- Fluorescent proteins for FRET : monitoring protein interactions in living cells / Richard N. Day -- Superresolution techniques / Michael W. Davidson -- The in vivo imaging revolution made by fluorescent proteins / Robert Hoffman.
  • Also available: Print – 2008
  • Folding for the Synapse addresses the current view on how protein folding and misfolding, controlled by molecular chaperones, contribute to synapse function and dysfunction. Molecular chaperones have been studied in relation to de novo protein folding, but there is increasing awareness that chaperone function is required for the regulation of protein dynamics when functioning physiologically as an isolated moiety or part of a protein complex. This book will introduce both important concepts of folding machineries and give examples of the biological relevance of further chaperone functions.
  • The cyclic ovary can be seen as a site of tissue damage, repair and precisely controlled tissue homeostasis, as long ovulation and luteolysis can be compared with acute and chronic inflammation. Innate immunity appears to be a powerful force in the endocrine system, representing a novel concept. In this monograph, remarkable evidence is given for the immune-privileged ovary being an implant on the chicken chorioallantoic membrane with an areactive mesenchyme. Mild to severe tissue damages due to follicular atresia, follicular rupture, or intraovarian oocyte release do no lasting harm. The most exciting part relates to the analysis of cytokeratin-positive (CK<sup>+</sup>) cells, comparing the fate mapping of this cell type from the fetal ovary to the adult organ. Findings on toll-like receptor 4 regulation and interferon-gamma-dependent positive effects indicate that CK<sup>+</sup> cells from human preovulatory follicles and bovine corpora lutea have similarities with nonlymphoid dendritic cells, a discovery that has the makings of a top story in basic and clinical research on the ovary.
  • A short dictionary of the Pap test -- Analogies in the Pap test : "Of corn flakes and raisins" -- Basic cytology principles -- Basic anatomy and cytology of the female genital tract -- The Bethesda system -- Liquid-based cytology -- Physiological cytology -- Reactive changes and organisms -- Human papillomavirus in cervical carcinogenesis -- Atypical squamous cells -- Low-grade squamous intra-epithelial lesion -- High-grade squamous intra-epithelial lesion -- Invasive squamous carcinoma -- Endocervical lesions -- Endometrial lesions -- Metastatic tumors -- Therapy-related changes -- Artifacts, contaminants, and incidental findings -- Screening and management guidelines of Pap test and newer techniques.
  • Also available: Print – 2012
    Fuzzy complexes : a more stochastic view of protein function / Monika Fuxreiter and Peter Tompa -- Dynamic fuzziness during linker histone action / Steven J. McBryant and Jeffrey C. Hansen -- Intrinsic protein flexibility in regulation of cell proliferation : advantages for signaling and opportunities for novel therapeutics / Ariele Viacava Follis, Charles A. Galea, and Richard W. Kriwacki -- Interplay between protein order, disorder and oligomericity in receptor signaling / Alexander B. Sigalov -- Consequences of fuzziness in the NFB/IB interaction / Elizabeth A. Komives -- Roles for intrinsic disorder and ̂fuzziness in generating context specific function in ultrabithorax : a hox transcription factor / Sarah E. Bondos and Hao Ching Hsiao -- Molecular recognition by the EWS transcriptional activation domain / Kevin A.W. Lee -- The measles virus NTAILXD complex : an illustrative example of fuzziness / Sonia Longhi -- Fuzziness in the core of the human pathogenic viruses HCV and HIV / Roland Ivanyi Nagy and Jean Luc Darlix -- Structural disorder and protein elasticity / Sarah Rauscher and Røgis Pomès -- "Fuzziness" in the cellular interactome: a historical perspective / G. Rickey Welch.
  • This Brief offers a concise, handy overview of the main concepts related to Embryology, revisited through the novel concepts that are applied daily in stem cell research and cell therapy oriented investigations. It is based on three main areas: -The process involved in female gamete differentiation and maturation. The main aspects related to cell biology will be covered and an overview of the epigenetic regulation of gametogenesis will be presented. -Early stages of embryo development with a careful analysis of the regulatory mechanisms driving cleavage, polarization and genome activation. -Stem cell and gametogenesis. The use of the oocyte as a possible source for the derivation of stem cell lines is discussed and depicted as a powerful tool to investigate oocyte potency and asymmetric imprinting. The potential biological implications are evaluated and use of stem cells to derive oocytes is presented.
  • "The gases termed gasotransmitters play an increasingly important role in understanding how signalling into and between cells is modulated and fine-tuned. The advent of gasotransmitters has profoundly changed our way of thinking about biosynthesis, liberation, storage and action mechanisms in cellular signaling. In recent years an impressive amount of new data, distributed throughout the existing literature, has been generated. For this book the editors have recruited distinguished colleagues in the field to summarize and review important biological, pharmacological and medical functions and their implications, as well as methods for the detection of gasotransmitters."--P. [4] of cover.
  • Also available: Print – 2006
  • Immunomagnetic enrichment of human and mouse hematopoietic stem cells for gene therapy applications / Guillermo Guenechea, Jose C. Segovia, and Juan A. Bueren -- Isolation of human and mouse hematopoietic stem cells / Yuk Yin Ng ... [et al.] -- Murine hematopoietic stem cell transduction using retroviral vectors / Ute Modlich ... [et al.] -- Genetic modification of human hematopoietic cells: preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials / Tulin Budak-Alpdogan and Isabelle Rivière -- Short-term culture of human CD34⁺ cells for lentiviral gene transfer / Francesca Santoni de Sio and Luigi Naldini -- T cell culture for gammaretroviral transfer / Sebastian Newrzela, Gunda Brandenburg, and Dorothee von Laer -- Retroviral transduction of murine primary T lymphocytes / James Lee, Michel Sadelain, and Renier Brentjens -- Lentiviral vector gene trasfer into human T cells / Els Verhoeyen, Caroline Costa, and Francois-Loic Cosset -- DNA transposons for modification of human primary T lymphocytes / Xin Huang ... [et al.] -- Retroviral gene transfer into primary human natural killer cells / Evran Alici, Tolga Sutlu, and M. Sirac Dilber -- Lentiviral vector-mediated genetic programming of mouse and human dendritic cells / Renata Stripecke -- In situ (in vivo) gene transfer into murine bone marrow stem cells / Dao Pan -- In vivo and ex vivo gene transfer in thymocytes and thymocyte precursors / Oumeya Adjali ... [et al.] -- Design and production of retro- and lentiviral vectors for gene expression in hematopoietic cells / Axel Schambach, William P. Swaney, and Johannes C.M. van der Loo -- Knock-down of gene expression in hematopoietic cells / Michaela Scherr, Letizia Venturiani, and Matthias Eder -- The use of retroviral vectors for tet-regulated gene expression in cell populations / Rainer Löw -- Detection of replication competent retrovirus and lentivirus / Lakshmi Sastry and Kenneth Cornetta -- Release testing of retroviral vectors and gene-modified cells / Diana Nordling, Anne Kaiser, and Lilith Reeves -- Copy number determination of genetically-modificed hematopoietic stem cells / Todd Schuesler ... [et al.] -- Tissue procurement for molecular studies using laser-assisted microdissection / Ulrich Lehmann and Hans Kreipe -- Leukemia diagnosis in murine bone marrow transplantation models / Zhixiong Li, Ute Modlich, and Anjali Mishra -- Humanized mouse models to study the human haematopoietic stem cell compartment / Dominique Bonnet -- Canine models of gene-modified hematopoiesis / Brian C. Beard and Hans-Peter Kiem -- Detection of retroviral integration sites by linear amplification-mediated PCR and tracking of individual integration clones in different samples / Manfred Schmidt ... [et al.] -- Retroviral insertion site analysis in dominant haematopoietic clones / Olga S. Kustikova, Ute Modlich, and Boris Fehse -- Tracking gene-modified T cells in vivo / Alessandra Recchia and Fulvio Mavilio -- DNA microarray studies of hematopoietic subpopulations / Karin Pike-Overzet ... [et al.] -- Quantification of genomic mutations in murine hematopoietic cells / Hartmut Geiger ... [et al.] -- Proteomics studies after hematopoietic stem cell transplantation / Eva M. Weissinger, Petra Zürbig, and Arnold Ganser -- Spectral karyotyping and fluorescence in situ hybridization of murine cells / Cornelia Rudolph and Brigitte Schlegelberger -- Database setup for preclinical studies of gene-modified hematopoiesis / Brenden Balcik, Elke Grassman, and Lilith Reeves -- The US and EU regulatory perspectives on the clinical use of hematopoietic stem/progenitor cells genetically modified ex vivo by retroviral vectors / Carolyn A. Wilson and Klaus Cichutek.
  • From microorganisms to humans, this volume provides an interdisciplinary overview of how genome integrity is maintained. It begins with DNA replication and continues with replicative DNA repair and pleiotropic protein interactions. It also reviews the cellular responses to radiation and genotoxic stress affecting whole genomes.
  • Also available: Print – 2013
  • Germline stem cells 2008
    Springer Protocols
    pt. I. Identification and regulation of germline stem cells in model organisms -- The development of germline stem cells in Drosophila / David A. Dansereau and Paul Lasko -- Analysis of the C. elegans germline stem cell region / Sarah L. Crittenden and Judith Kimble -- Immunohistological techniques for studying the Drosophila male germline stem cell / Shree Ram Singh and Steven X. Hou -- Genetic tools used for cell lineage tracing and gene manipulation in Drosophila germline stem cells / Wei Liu and Steven X. Hou -- Structural polarity and dynamics of male germline stem cells in an insect (milkweed bug Oncopeltus fasciatus) / David C. Dorn and August Dorn -- High-resolution light microscopic characterization of spermatogonia / Hélio Chiarini-Garcia and Marvin L. Meistrich -- Identification and characterization of spermatogonial subtypes and their expansion in whole mounts and tissue sections from primate testes / Jens Ehmcke and Stefan Schlatt -- Epigenetic control in male germ cells : a transillumination-assisted microdissection method for the analysis of developmentally regulated events / Durga Prasad Mishra and Paolo Sassone-Corsi -- GDNF maintains mouse spermatogonial stem cells in vivo and in vitro / Hannu Sariola and Tiina Immonen -- g pt. II. In vitro culture and application of germline stem cells -- Ectopic grafting of mammalian testis tissue into mouse hosts / Ina Dobrinski and Rahul Rathi -- Spermatogonial stem cell transplantation, testicular function, and restoration of male fertility in mice / Derek J. McLean -- Isolating highly pure rat spermatogonial stem cells in culture / F. Kent Hamra ... [et al.] -- Deriving mouse spermatogonial stem cell lines / Ilaria Falciatori ... [et al.] -- Production of knockdown rats by lentiviral transduction of embryos with short hairpin RNA transgenes / Christina Tenenhaus Dann and David L. Garbers -- Testicular germ cell tumors in mice : new ways to study a genetically complex trait / Jason D. Heaney and Joseph H. Nadeau -- Study origin of germ cells and formation of new primary follicles in adult human and rat ovaries / Antonin Bukovsky ... [et al.].
  • This volume provides a compact, concise and up-to-date resource for pathologists and clinicians who are involved in women's health. The book summarizes the incidence, epidemiology and risk factors for cervical adenocarcinoma, while reviewing the normal histology of the cervix and glandular lesions of the cervix. The cytological appearance of glandular lesions of the cervix and their mimics is amply illustrated and discussed. Management of glandular lesions is also outlined. Written by authorities in the field, Glandular Lesions of the Uterine Cervix: Cytopathology with Histologic Correlates is of great value to cytopathologists, surgical pathologists, gynecologic oncologists and surgeons.
  • Gliogenesis -- Astroglia -- Oligodendroglia -- Microglia -- Ependymal cells -- Tanycytes -- Schwann cells.
  • Glioma signaling 2013
    Also available: Print – 2013
  • Also available: Print – 2011
    Heparan sulfate proteoglycan in inflammation and angiogenesis / Lianchun Wang -- Heparan sulfate proteoglycans in infection / Allison H. Bartlett and Pyong Woo Park -- Glycans in cancer / Lubor Borsig -- Glycosaminoglycans in atherosclerosis and thrombosis / C.P. Vicente, J.A.P. Godoy, and C.C. Werneck -- Glycans in embryonic stem cells / Claire Pickford ... [et al.] -- Heparan sulfate proteoglycans in cancer therapy / Ezeddin Salem Gassar, Sherif A. Ibrahim, and Martin Götte -- Structure and biological activities of glycosaminoglycan analogs from marine invertebrates : new therapeutic agents? / Eliene O. Kozlowski ... [et al.]
  • Also available: Print – 2010
  • Also available: Print – 2010
  • Handbook of dynein 2012
    "This book draws together recent data on both cytoplasmic and flagellar dyneins and the proteins they interact with, to give readers a clear picture of what is currently known about the structure and mechanics of these remarkable macro-molecular machines. Each chapter is written by active researchers, with a focus on currently used biophysical, biochemical, and cell biological methods. This is a useful handbook for frontline researchers as well as a textbook for advanced students."--P. [4] of cover.
  • "Reflecting the milestones in the field that have occurred since the publication of the first edition, this book provides an updated tool kit that incorporates conventional as well as modern approaches to tackling biological and medicinal research in the post genomic era. The authors have evaluated, revised, and sometimes replaced all protocols with more efficient, reliable, or simpler ones. The book is also completely reorganized and covers topics of modern "omics" approaches, including genomics, proteomics, and metabolomics. This is followed coverage of the Manipulation of Biological Systems, including RNAi, and the use of microscopy"--Provided by publisher.
  • In a conceptually current, quick-reference, Question & Answer format, the second edition of Handbook of Practical Immunohistochemistry: Frequently Asked Questions continues to provide a comprehensive and yet concise state-of-the-art overview of the major issues specific to the field of immunohistochemistry. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 10,000 tumors and normal tissue to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. ℗ℓ Fully revised and updated from the first edition, the new features of the second edition include over 200 additional questions or revised questions with an IHC panel to answer each question; over 250 new color photos and illustrations; over 20 new useful biomarkers; hundreds of new references; several new chapters to cover phosphoproteins, rabbit monoclonal antibodies, multiplex IHC stains, overview of predictive biomarkers, and integration of IHC into molecular pathology; many new coauthors who are international experts in a related field; many updated IHC panels using Geisinger IHC data collected from over 10,000 tumors and normal tissues; and updated appendices containing detailed antibody information for both manual and automated staining procedures. ℗ℓ Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, Second Edition will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.
  • Quality management and regulation / Jeffrey Prichard -- Technique and troubleshooting of antibody testing / Fan Lin and Jianhui Shi -- Overview of automated immunohistochemistry / Jeffrey Prichard, Angela Bitting, and Joe Myers -- Automated staining : Dako perspective / Ole F. Rasmussen and Andreas Schønau -- Automated staining : Ventana perspective / Angela Sattler -- Tissue microarray / Myra Wilkerson and Erin Powell -- Unknown primary/undifferentiated neoplasms in surgical and cytologic specimens / Fan Lin and Haiyan Liu -- Exfoliative cytopathology / Steven Meschter and Jan F. Silverman -- Predictive markers of breast cancer : ER, PR, and HER2 / Jeffrey Prichard, David Hicks, and Elizabeth Hammond -- Central and peripheral nerve system tumors / Hueizhi (Hope) Wu, Conrad Schuerch, and Douglas C. Miller -- Thyroid and parathyroid gland / Haiyan Liu, Fan Lin, and Ronald A. DeLellis -- Adrenal gland / Hanna G. Kaspar -- Salivary gland and other head and neck structures / Conrad Schuerch -- Lung, pleura, and mediastinum / Kai Zhang and Phillip Cagle -- Breast / Haiyan Liu -- Uterus / Hanna G. Kaspar -- Ovary / Jeffrey Prichard, Haiyan Liu, and Myra Wilkerson -- Prostate gland / Haiyan Liu, Fan Lin, and Qihui (Jim) Zhai -- Urinary bladder / Myra Wilkerson -- Kidney / Fan Lin and Ximing J. Yang -- Testis and paratesticular tissues / Myra Wilkerson -- Pancreas and ampulla / Fan Lin and Hanlin L. Wang -- Liver, bile ducts, and gallbladder / Jeffrey Prichard and Fan Lin -- Upper gastrointestinal tract / Jinhong Li and Fan Lin -- Lower gastrointestinal tract and microsatellite instability / Jinhong Li and Fan Lin -- Soft tissue and bone tumors / Shaobo Zhu and Markku Miettinen -- Lymph node / Xiaohong (Mary) Zhang and Nadine S. Aguilera -- Bone marrow / R. Patrick Dorion and Xiaohong (Mary) Zhang -- Infectious diseases / Dirk M. Elston, Lawrence E. Gibson, and Heinz Kutzner -- Skin / Tammie Ferringer -- Application of direct immunofluorescence for the skin and mucosal biopsies : a practical review / William B. Tyler -- In situ hybridization in surgical and cytologic specimens / Hong Yin and Barbara Paynton.
  • Handbook of stem cells 2004
    Also available: Print – v. 1-2, 2004.
    Vol. 1. Embryonic stem cells -- Vol. 2. Adult and fetal stem cells.
  • Also available: Print – 2006
    Origin and fate of hematopoietic precursors in the early mouse embryo / Julien Yuan Bertrand ... [et al.] -- Hematopoietic development in drosophila: a parallel with vertebrates / Marie Meister and Shubha Govind -- Intraembryonic development of hematopoietic stem cells during human ontogeny: expression analysis / Caroline Marshall -- Gene and cell therapy involving hematopoietic stem cell / Isabelle André-Schmutz and Marina Cavazzana-Calvo. Ventral and dorsal contributions to hematopoiesis in xenopus / Aldo Ciau-Uitz, Maggie Walmsley and Roger Patient -- Genetic dissection of hematopoiesis using the zebrafish / Rebecca A. Wingert and Leonard I. Zon -- Extra- and intraembryonic HSC commitment in the avian model / Thierry Jaffredo ... [et al.] -- Avian lymphopoiesis and transcriptional control of hematopoietic stem cell differentiation / Jussi Liippo and Olli Lassila -- Yolk sac development in mice / James Palis -- Long-term reconstituting hematopoietic stem cell capacity in the embryo / Mervin C. Yoder -- The endothelium: the cradle of definitive hematopoiesis? / Katrin Ottersbach and Elaine Dzierzak -- Endothelial and hematopoietic cells in the intraembryonic compartment / Fumio Arai and Toshio Suda --
  • Also available: Print – 2009
  • Also available: Print – 2008
    Stem cell biology / Elizabeth O. Hexner and Stephen G. Emerson -- Stem cell transplantation for hematologic malignancies / Effie W. Petersdorf -- Immunobiology of transplantation / Robert S. Negrin -- Allogeneic and autologous hematopoietic stem cell transplantation for acute lymphoblastic leukemia and acute myelogenous leukemia in the adult / Anthony Stein and Stephen J. Forman -- Hematopoietic cell transplantation for chronic myeloid leukemia / Uday Popat and Sergio Giralt -- Stem cell transplantation for Hodgkin's and non-Hodgkin's lymphomas / John W. Sweetenham -- Transplantation in chronic lymphocytic leukemia / Rifca Le Diew and John G. Gribben -- Stem cell transplantation in multiple myeloma / John Koreth and Kenneth C. Anderson -- Allogeneic hematopoietic cell transplantation for patients with myelodysplastic syndrome and myeloproliferative disorders / Aravind Ramakrishnan and H. Joachim Deeg -- Stem cell transplantation for marrow failure / Philippe Armand and Joseph H. Antin -- Stem cell transplantation for hemoglobinopathies / Catherine J. Wu -- Stem cell sources for allogeneic transplantation / Corey Cutler -- Allogeneic unrelated donor blood and marrow transplantation / Daniel Weisdorf -- Umbilical cord hematopoietic stem cell transplantation / Mitchell E. Horwitz and Nelson Chao -- Haploidentical stem cell transplantation / Bimalangshu R. Dey and Thomas R. Spitzer -- Ablative preparative regimens for hematopoietic stem cell transplantation / Amin Alousi and Marcos de Lima -- Nonmyeloablative transplantation / Frédéric Baron and Brenda M. Sandmaier -- Immune reconstitution after allogeneic transplantation / Carolyn A. Keever-Taylor -- Infection in the hematopoietic stem cell transplant recipient / Francisco M. Marty and Lindsey R. Baden -- EBV lymphoproliferative disease after transplantation / Stephen Gottschalk, Cliona M. Rooney, and Helen E. Heslop -- Hepatic veno-occlusive disease / Erica Linden and Paul Richardson -- Lung injury after hematopoietic stem cell transplantation / Kenneth R. Cooke and Gregory Yanik -- Quality of life issues post-transplantation / Stephanie J. Lee -- Pathophysiology of acute graft-versus-host disease / Pavan Reddy and James L.M. Ferrara -- Acute graft-versus-host disease / Martin Benesch and H. Joachim Deeg -- Chronic Graft-versus-host disease / Javier Bolaños-Meade and Georgia B. Vogelsang -- Graft manipulation : T cell depletion and tumor cell purging / Vincent T. Ho -- Donor lymphocyte infusions : clinical applications and the graft-versus-leukemia effect / Edwin P. Alyea -- Regenerative medicine : application in cardiovascular diseases / Emerson C. Perin, Guilherme V. Silva, and Marlos R. Fernandes.
  • Human hepatocyte transplantation overview / Juliana Puppi and Anil Dhawan -- Isolation of human hepatocytes / Ragai R. Mitry -- An optimised method for cryopreservation of human hepatocytes / Claire Terry and Robin D. Hughes -- Liver cell culture techniques / José V. Castell and María José Gómez-Lechón -- In-vitro assays for induction of drug metabolism / Brian G. Lake ... [et al.] -- Hepatocyte apoptosis / Mustapha Najimi, Françoise Smets, and Etienne Sokal -- Small animal models of hepatocyte transplantation / Jurgen Seppen, Ebtisam El Filali, and Ronald Oude Elferink -- Hepatocyte transplantation techniques : large animal models / Anne Weber, Marie Thérèse Groyer-Picard, and Ibrahim Dagher -- Cell transplant techniques : engraftment detection of cells / Robert A. Fisher and Valeria R. Mas -- Hepatic preconditioning for transplanted cell engraftment and proliferation / Yao-Ming Wu and Sanjeev Gupta -- Ex vivo gene transfer into hepatocytes / Xia Wang ... [et al.] -- Sources of adult hepatic stem cells : haematopoietic / Rosemary Jeffery, Richard Poulsom, and Malcolm R. Alison -- Production of hepatocyte-like cells from human amnion / Toshio Miki ... [et al.] -- Generation of hepatocytes from human embryonic stem cells / Niloufar Safinia and Stephen L. Minger -- Isolation, in vitro cultivation, and characterisation of foetal liver cells / Yue Wu, Chetan C. Shatapathy, and Stephen L. Minger -- Human intrahepatic biliary epithelial cell lineages : studies in vitro / Ruth Joplin and Stivelia Kachilele -- Liver cell labelling with MRI contrast agents / Michel Modo, Thomas J. Meade, and Ragai R. Mitry -- Microbiological monitoring of hepatocyte isolation in the GMP laboratory / Sharon C. Lehec.
  • 1. General review on in vitro hepatocyte models and their applications / Christiane Guguen-Guillouzo and Andre Guillouzo -- 2. Human foetal hepatocytes / Anne Weber ... [et al.] -- 3. Isolation and culture of primary hepatocytes from resected human liver tissue / Edward L. LeCluyse and Eliane Alexandre -- 4. Optimisation of the cryopreservation of primary hepatocytes / Nicola J. Hewitt -- 5. Cryopreservation of human hepatocytes for clinical use / Ragai R. Mitry, Sharon C. Lehec, and Robin D. Hughes -- 6. Hepatocyte differentiation / Katy M. Olsavsky Goyak, Elizabeth M. Laurenzana, and Curtis J. Omiecinski -- 7. Reversible manipulation of apoptosis sensitivity in cultured hepatocytes by matrix-mediated manipulation of signaling activities / Patricio Godoy ... [et al.] -- 8. Markers and signaling factors for stem cell differentiation to hepatocytes / Frédéric Lemaigre -- 9. Hepatic stem cells / Robert E. Schwartz and Catherine Verfaillie -- 10. Hepatic stem cells and liver development / Nalu Navarro-Alvarez, Alejandro Soto-Gutierrez, and Naoya Kobayashi -- 11. Generation of hepatocytes from human embryonic stem cells / Neta Lavon -- 12. Isolation and culture of adult human liver progenitor cells / Sabine Gerbal-Chaloin ... [et al.] -- 13. The HepaRG cell line / Marie-Jeanne Marion, Olivier Hantz, and David Durantel -- 14. Transdifferentiation of pancreatic cells to hepatocytes / Chia-Ning Shen and David Tosh -- 15. Evaluation of drug metabolism, drug-drug interactions and in vitro hepatotoxicity with cryopreserved human hepatocytes / Albert P. Li -- 16. The use of human hepatocytes to investigate drug metabolism and CYP enzyme induction / Sylvie Klieber ... [et al.] -- 17. The use of hepatocytes to investigate UDP-glucuronosyltransferases and sulfotransferases / Sylvie Fournel-Gigleux ... [et al.] -- 18. The use of hepatocytes to investigate drug uptake transporters / Kazuya Maeda and Yuichi Sugiyama -- 19. Metabonomic studies on human hepatocyte in primary culture / Vincent Croizmarie ... [et al.] -- 20. The application of HepRG cells in evaluation of cytochrome P450 induction properties of drug compounds / Tommy B. Andersson -- 21. The use of hepatocytes to investigate drug toxicity / María José Gómez-Lechón, José V. Castell, Maria Teresa Donato -- 22. The use of human hepatocytes to investigate bile acid synthesis / Ewa C.S. Ellis and Lisa-Mari Nilsson -- 23. The use of human hepatocytes to investigate blood coagulation factor / Christine Biron-Andréani ... [et al.] -- 24. Use of human hepatocytes to investigate HCV infection / Lydiane Pichard-Garcia ... [et al.] -- 25. The use of hepatocytes to investigate HDV infection / Camille Sureau -- 26. Rodent models of liver repopulation / Helène Gilgenkrantz -- 27. Chimeric mice with humanized liver / Stephen C. Strom, Julio Davila, and Markus Grompe -- 28. Bioartificial liver support systems / Gesine Pless -- 29. Human hepatocyte transplantation / Anil Dhawan ... [et al.].
  • Also available: Print – 2014
    This volume highlights the most interesting biomedical and clinical applications of high-dimensional flow and mass cytometry. It reviews current practical approaches used to perform high-dimensional experiments and addresses key bioinformatic techniques for the analysis of data sets involving dozens of parameters in millions of single cells. Topics include single cell cancer biology; studies of the human immunome; exploration of immunological cell types such as CD8+ T cells; decipherment of signaling processes of cancer; mass-tag cellular barcoding; analysis of protein interactions by proximity ligation assays; Cytobank, a platform for the analysis of cytometry data; computational analysis of high-dimensional flow cytometric data; computational deconvolution approaches for the description of intracellular signaling dynamics; and hyperspectral cytometry. All 10 chapters of this book have been written by respected experts in their fields. It is an invaluable reference book for both basic and clinical researchers.
  • Print Material
  • The Hippo signaling pathway is rapidly gaining recognition as an important player in organ size control and tumorgenesis. This volume presents virtually all aspects of tumor biology because members of the Hippo Pathway have been associated with numerous well-established cell signaling pathways, just to name a few; Rho, Wnt, TGFbeta and p53. Hippo signaling is not solely involved in regulating "classic" tumor characteristics such as cell proliferation, survival and growth, but is also diversely involved in cell-autonomous and non-cell-autonomous differentiation, migration and organ size control. With the multitude of signaling events mediated by the Hippo pathway and the vastly different functions that it plays, it is evident that these tumor suppressors are unique governors of cellular homeostasis. This timely volume gathers wide-ranging and burgeoning information on the Hippo pathway and its role in cancer into an accessible format of a single book.With the multitude of signaling events mediated by the Hippo pathway and the vastly different functions that it plays, it is evident that these tumor suppressors are unique governors of cellular homeostasis. This timely volume gathers wide-ranging and burgeoning information on the Hippo pathway and its role in cancer into an accessible format of a single book.
  • Section 1. Histological and histochemical methods with light microscopy -- section 2. Cytological methods in electron microscopy -- section 3. Image quantification.
  • Pt 1. Fundamental concepts -- pt 2. The four basic tissue types -- pt 3. Organs and organ systems .
  • Epithelium -- Epithelial glands -- Cell signaling -- Connective tissue -- Osteogenesis -- Blood and hematopoiesis -- Muscle tissue -- Nervous tissue -- Sensory organs vision and hearing -- Immune-lymphatic system -- Integumentary system -- Cardiovascular system -- Respiratory system -- Urinary system -- Upper digestive system -- Lower digestive system -- Digestive glands -- Neuroendocrine system -- Endocrine system -- Spermatogenesis -- Sperm transport and maturation -- Follicle development and the menstrual cycle -- Fertilization, placentation, and lactation.
  • Also available: Print – 2013
  • Also available: Print – 2009
    Preface -- Host restriction of HIV-1 by APOBEC3 and viral evasion through Vif -- Interactions of viral protein U (Vpu) with cellular factors -- TRIM5alpha -- Human immunodeficiency virus type-1 Gag and host vesicular trafficking pathways -- The roles of tetraspanins in HIV-1 replication -- Imaging of HIV/host protein interactions -- Virological and cellular roles of the transcriptional coactivator LEDGF/p75 -- Implications of Nef: host cell interactions in viral persistence and progression to AIDS -- Vpr and its interactions with cellular proteins.
  • Human homeostasis refers to complex interactions to regulate and maintain the internal conditions of the organism under stable condition. In spite of the progress made on cell growth and division, the circuits that coordinate these processes and maintain the homeostatic balance between cell growth and cell death have not been clarified. Deregulation of homeostatic processes results in different forms of imbalances that can turn to diseases including hepatitis, cyrrhosis and tumor formation. Abnormal growth of tissues can lead to a broad spectrum of neoplastic diseases from tissue swelling, primary tumor formation, cancer and ultimately to secondary tumors known as metastases. Tumor formation is a complex process, that develops through many steps. The discussion of balance and its loss in cell growth, primary and secondary tumor formation necessitate basic information in chemistry (carcinogenesis), biochemistry (metabolism), physics (instrumentation), cell biology (compartments), anatomy, physiology, clinical experience (screening, medical imaging, therapy). The focus of this book has been placed on: a) balance of cell growth, b) tumor formation, c) development of cancer and metastasis, e) diagnosis and treatment of cancer. The steps of the lymphatic type of metastatic tumor spread are described. Due to the interdisciplinary nature, the book gives information for a wide audience including biology, biotechnology, molecular, ecology, medical, pharmacist, chemist students. Additional information is provided for biomedical, pharma, medical and life scientists, physicians and health care professionals.
  • 1. Neural progenitors / Dustin R. Wakeman ... [et al.] -- 2. Multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.] -- 3. Endothelium / Sangmo Kwon, Takayuki Asahara -- 4. Lung / Rabindra Tirouvanziam, Megha Makam, Bruno Péault -- 5. Eye / Maria Notara ... [et al.] -- 6. Colon / F. Iovino ... [et al.] -- 7. Spermatogonia / Makoto C. Nagano, Jonathan R. Yeh, Khaled Zohni -- 8. Hair follicle pluripotent stem (hfPS) cells / Robert M. Hoffman -- 9. Pancreas / Fang-Xu Jiang, Grant Morahan -- 10. Prostate / C. Foley ... [et al.].
  • Establishment and maintenance of normal human keratinocyte cultures / Claire Linge -- Cultivation of normal human epidermal melanocytes in the absence of phorbol esters / Mei-Yu Hsu, Ling Li, and Meenhard Herlyn -- Isolation and culture of human osteoblasts / Alison Gartland ... [et al.] -- Human osteoclast culture from peripheral blood monocytes : phenotypic characterization and quantitation of resorption / Katherine A. Buckley ... [et al.] -- Human chondrocyte cultures as models of cartilage-specific gene regulation / Mary B. Goldring -- Human myoblasts and muscle-derived SP cells / Grace K. Pavlath and Emanuela Gussoni -- Cell cultures of autopsy-derived fibroblasts / Volker Meske, Frank Albert, and Thomas G. Ohm -- Primary culture and differentiation of human adipocyte precursor cells / Vanessa van Harmelen, Thomas Skurk, and Hans Hauner -- Human mononuclear phagocytes in tissue culture / Yona Keisari -- Purification of peripheral blood natural killer cells / Bice Perussia and Matthew J. Loza -- Human fetal brain cell culture / Mark P. Mattson -- Culturing human schwann cells / Victor J. Turnbull -- Well-differentiated human airway epithelial cell cultures / M. Leslie Fulcher ... [et al.] -- Isolation and culture of human alveolar epithelial cells / Carsten Ehrhardt, Kwang-Jin Kim, and Claus-Michael Lehr -- A new approach to primary culture of human gastric epithelium / Pierre Chailler and Daniel Ménard -- Isolation and culture of human colon epithelial cells using a modified explant technique employing a noninjurious approach / Hamid A. Mohammadpour -- Isolation and culture of human hepatocytes / Martin Bayliss and Graham Somers -- Glomerular epithelial and mesangial cell culture and characterization / Heather M. Wilson and Keith N. Stewart -- Isolation and culture of human renal cortical cells with characteristics of proximal tubules / Gabrielle M. Hawksworth -- Culture of parathyroid cells / Per Hellman -- Long-term culture and maintenance of human islets of langerhans in Memphis serum-free media / Daniel W. Fraga, A. Osama Gaber, and Malak Kotb -- Primary culture of human antral endocrine and epithelial cells / Susan B. Curtis and Alison M. J. Buchan -- Conjunctiva organ and cell culture / Monica Berry and Marcus Radburn-Smith -- Establishment and maintenance of in vitro cultures of human retinal pigment epithelium / Martin J. Stevens.
  • Also available: Print – 2011
    Pt. 1. Multistep models -- pt. 2. Prostate cancer/stem cells -- pt. 3. Breast cancer/stem cells -- pt. 4. Multistep/genes.
  • Human embryonic stem cells: isolation, maintenance and differentiation / Kursad Turksen and Tammy-Claire Troy -- Blastocyst culture for deriving human embryonic stem cells / Ariff Bongso -- Propagation of human embryonic stem cells on human feeder cells / Mark Richards and Ariff Bongso -- Derivation and maintenance of human embryonic stem cells / Michal Amit and Joseph Itskovitz-Eldor -- Isolation and characterization of human embryonic stem cells / Maisam Mitalipova and Gail Palmarini -- Routine culture and differentiation of human embryonic stem cells / Jim McWhir, Davina Wojtacha, and Alison Thomson -- Culture of human embryonic stem cells on human and mouse feeder cells / Gautam Dravid, Holly Hammond, and Linzhao Cheng -- Maintenance of human embryonic stem cells in animal serum- and feeder layer- free culture conditions / Michal Amit and Joseph Itskovitz-Eldor -- Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor / Noboru Sato and Ali H. Brivanlou -- Derivation of human feeders for prolonged support of human embryonic stem cells / Chui-Yee Fong and Ariff Bongso -- Transplantation of human embryonic stem cells to the chick embryo / Ronald S. Goldstein -- Derivation and characterization of neuronal precursors and dopaminergic neurons from human embryonic stem cells in vitro / Melissa Carpenter ... [et al.] -- In vitro differentiation of neural precursors from human embryonic stem cells / Xue-Jun Li and Su-Chun Zhang -- Derivation and characterization of hematopoietic cells from human embryonic stem cells / Lisheng Wang ... [et al.] -- Retroviral transduction of hematopoietic progenitors derived from human embryonic stem cells / Pablo Menendez ... [et al.] -- Genetic manipulation of human embryonic stem cells by transfection / Rachel Eiges -- Designing, testing, and validating a microarray for stem cell characterization / Yongquan Luo ... [et al.] -- Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells / Noboru Sato and Ali H. Brivanlou -- Massively parallel signature sequencing / Daixing Zhou ... [et al.] -- Confrontation cultures of embryonic stem cells with multicellular tumor spheroids to study tumor-induced angiogenesis / Maria Wartenberg ... [et al.] -- A novel experimental platform for investigating cancer growth and anti-cancer therapy in a human tissue microenvironment derived from human embryonic stem cells / Maty Tzukerman and Karl L. Skorecki -- The analysis of mitochondria and mitochondrial DNA in human embryonic stem cells / Justin C. St. John ... [et al.].
  • Association between cancer and "acid mucopolysaccharides" : an old concept comes of age, finally -- Hyaluronan-CD44 interactions and chemoresistance in cancer cells -- Growth factor regulation of hyaluronan deposition in malignancies -- Hyaluronan binding protein 1 (HABP1/p32/gClqR) : a new perspective in tumor development -- CD44 meets Merlin and Ezrin : their interplay mediates the pro-tumor activity of CD44 and tumor-suppressing effect of Merlin -- Hyaluronan-mediated CD44 interaction with receptor and non-receptor kinases promotes oncogenic signaling, cytoskeleton activation, and tumor progression -- Adhesion and penetration : two sides of CD44 signal transduction cascades in the context of cancer cell metastasis -- Involvement of CD44, a molecule with a thousand faces, in cancer dissemination -- RHAMM/HMMR : an itinerant and multifunctional hyaluronan binding protein that modifies CD44 signaling and mitotic spindle formation -- Altered hyaluronan biosynthesis in cancer progression -- Hyaluronidase : both a tumor promoter and suppressor -- The hyaluronidases in cancer biology -- Hyaluronan fragments : informational polymers commandeered by cancers -- Hyaluronan in human tumors : importance of stromal and cancer cell-associated hyaluronan -- The oncofetal paradigm revisited : MSF and HA as contextual drivers of cancer progression -- Hyaluronan synthesis and turnover in prostate cancer -- Role of hyaluronan in melanoma progression -- Role of hyaluronan metabolism in the initiation, invasion, and metastasis of breast cancer -- Clinical use of hyaluronidase in combination cancer chemotherapy : a historic perspective -- Exploring the hyaluronan:CD44 interaction for cancer therapy -- Hyaluronidase-2 and its role as a cell-entry receptor for sheep retroviruses that cause contagious respiratory tract cancers.
  • Adult neurogenesis has been questioned for many years. In the early 1900s, a dogma was established that denied new neuron formation in the adult brain. Adult neural stem cells are undifferentiated cells that are present in the adult brain and are capable of dividing and differentiating into glia and new neurons.
  • Also available: Print – 2013
    The knowledge of Th17 cells and other cell populations which secrete IL-17A, and/or IL-22 has expanded tremendously since the publication of the first edition "Th17 Cells: Role in Inflammation and Autoimmune Disease" in 2008. The present volume has been completely revised with the addition of new chapters on the IL-17 receptor family and signaling, and an in-depth review of IL-22 and innate lymphoid cells. The differentiation of naïve T cells into regulatory T cells and Th17 cells as well as the plasticity of Th17 cells is discussed.
  • Recent years have witnessed numerous advances in stem cell research that have suggested novel strategies for the treatment of the chronic and regenerative diseases that pose a growing challenge to the provision of healthcare in the 21<sup>st</sup> century. Nevertheless, there is little doubt that related disciplines have failed to keep pace with such developments, of which the immunology of transplant rejection is perhaps the most relevant to the nascent field of regenerative medicine. Indeed, given that after decades of research into the mechanisms of organ allograft rejection, we remain wholly dependent on immune suppression to ensure acceptance of foreign tissues, we might legitimately ask whether there is any reason why tissues differentiated from stem cells might not suffer the same fate. This volume seeks to address this issue by drawing on the principles of alloantigen recognition to assess the validity of early convictions that embryonic stem cell-derived tissues may prove invisible to the immune system of the host and the more recent belief that induced pluripotent stem cells, derived in a fully autologous manner, will circumvent the issue of immunogenicity. Having debunked such assumptions and established the magnitude of the immunological barriers to be overcome, this book then draws on the expertise of internationally-recognised laboratories around the world to provide a critical assessment of the likely success of applying strategies such as stem cell banking, silencing of genes that confer immunogenicity, and the induction of transplantation tolerance to overcoming the immunological barriers in the future. Indeed, it is only through reaching a consensus in the field that enduring solutions to such an intractable problem may ultimately emerge.
  • 1. VEGF inhibition increases hematocrit by hepatic Hif-2[Alpha]-dependent erythrocytosis and Hif-2[Alpha]--independent plasma volume contraction. Vascular Endothelial Growth Factor (VEGF) is an essential regulator of angiogenesis whose pharmacological inhibition represents a promising cancer therapeutic modality. We have previously reported that high-grade inhibition of VEGF by adenoviral expression of soluble VEGF receptor (VEGFR) ectodomains and recombinant VEGF Trap protein induce hepatic Epo-dependent erythrocytosis and polycythemia. Here, we demonstrate that VEGF inhibition induces erythrocytosis in a hepatic Hif-2[Alpha]-dependent manner. Conditional inactivation of hepatic Hif-2[Alpha] in Hif2[Alpha] flox/flox mice by either adenovirus Cre injection or albumin-Cre genetic cross suppressed VEGF inhibition-induced hepatic Epo production and blunted hematocrit elevation. Interestingly, a modest rise in hematocrit (5-10%) persisted in VEGF inhibitor-treated Hif-2[Alpha] liver knockout mice despite the absence of hepatic Epo elevation or reticulocytosis. Analysis of red blood cell mass and plasma volume indicate that, in addition to erythrocytosis, VEGF inhibition significantly reduces blood plasma volume (PV) (10-30% decrease) in a EPO- and Hif-2[Alpha]-independent manner. Thus, VEGF inhibition-induced hematocrit elevation in mice is attributable to both hepatic Hif-2[Alpha]-dependent erythrocytosis and Hif-2[Alpha] --independent plasma volume contraction, with accompanying implications for human patients exhibiting elevated haemoglobin levels in response to pharmacologic VEGF inhibition. 2. VEGF Inhibitors Enhance Hepatic insulin Signaling and Ameliorates Diabetes through Activation of Hepatic HIF-2[Alpha]. In the United States, over 18 million people have diabetes and approximately 90% of these cases are the type 2 form of the disease. The central pathophysiologic defect of T2DM is insulin resistance, where tissues such as muscle, fat and liver exhibit reduced responsiveness to insulin, despite normal to elevated levels of production of the hormone. During our studies on the role of vascular endothelial growth factor (VEGF) in erythropoeisis, we made a surprising observation that VEGF inhibition reduces blood glucose in normal lean mice and significantly corrects hyperglycemia in diabetic and insulin-resistant db/db mice. Metabolic profiling of VEGF inhibitor-treated mice revealed enhanced insulin sensitivity at the liver but not at the periphery. In the liver of these mice, expression of insulin receptor substrate-2 (IRS-2) was elevated, accompanied by enhanced Akt activation and suppression of gluconeogenic genes. Hepatic insulin sensitization by VEGF inhibitors requires hypoxia-inducible factor 2-alpha (Hif-2[Alpha]) as genetic deletion of hepatic Hif-2[Alpha] abrogated insulin sensitization and normalizes blood glucose in VEGF inhbitor-treated mice. Conversely, overexpression of a constitutively active allele of Hif-2[Alpha] in liver hepatocytes reduces blood glucose and activates hepatic insulin signaling in vitro and in vivo. Analysis of the human Irs-2 sequence revealed the presence of several potential Hypoxia-Responsive-Elements (HREs) within its 5' promoter. Using a Irs-2 promoter reporter construct, we found that overexpression of Hif-2[Alpha] or addition of a hypoxia-mimetic drug deferoxamine (DFO) resulted in increased Irs-2 transcription. These data suggest Irs-2 is a hypoxia-inducible gene. In this study, we show that VEGF inhibitors improve hepatic insulin action through activation of hepatic HIF-2[Alpha]. Our results suggest Hif-2[Alpha] positively regulates insulin signaling by promoting Irs-2 expression. These data suggest a previously unsuspected link between hypoxia and insulin signaling in the liver and indicate VEGF inhibitors may be useful for the treatment of diabetes.
  • 1. Introduction -- 2. Self-field theory -- 3. Biological and cosmological evolution -- 4. Biophotons and diffusion in biology -- 5. Cell division and membrane diffusion -- 6. Electromagnetic and acoustic-based therapies.
  • "Cell signaling, which is also often referred to as signal transduction or, in more specialized cases, transmembrane signaling, is the process by which cells communicate with their environment and respond temporally to external cues that they sense there. All cells have the capacity to achieve this to some degree, albeit with a wide variation in purpose, mechanism, and response. At the same time, there is a remarkable degree of similarity over quite a range of species, particularly in the eukaryotic kingdom, and comparative physiology has been a useful tool in the development of this field. The central importance of this general phenomenon (sensing of external stimuli by cells) has been appreciated for a long time, but it has truly become a dominant part of cell and molecular biology research in the past three decades, in part because a description of the dynamic responses of cells to external stimuli is, in essence, a description of the life process itself. This approach lies at the core of the developing fields of proteomics and metabolomics, and its importance to human and animal health is already plainly evident"--Provided by publisher.
  • 1. Back to the future: nuclear magnetic resonance and bioinformatics studies on intrinsically disordered proteins -- 2. Structure and dynamics of intrinsically disordered proteins -- 3. NMR methods for the study of intrinsically disordered proteins structure, dynamics, and interactions: general overview and practical guidelines -- 4. Ensemble calculation for intrinsically disordered proteins using NMR parameters -- 5. NMR spectroscopic studies of the conformational ensembles of intrinsically disordered proteins -- 6. Recombinant intrinsically disordered proteins for NMR: tips and tricks -- 7. Biophysical methods to investigate intrinsically disordered proteins: avoiding an "elephant and blind men" situation -- 8. Application of SAXS for the structural characterization of IDPs -- 9. Bioinformatics approaches for predicting disordered protein motifs -- 10. Towards understanding protein disorder in-cell -- 11. The Protein Ensemble Database -- 12. Order and disorder in the replicative complex of paramyxoviruses -- 13. Druggability of intrinsically disordered proteins -- 14. Beta amyloid hallmarks: from intrinsically disordered proteins to Alzheimer's Disease.
  • An Introduction to Biological Membranes: From Bilayers to Rafts covers many aspects of membrane structure/function that bridges membrane biophysics and cell biology. Offering cohesive, foundational information, this publication is valuable for advanced undergraduate students, graduate students and membranologists who seek a broad overview of membrane science. Brings together different facets of membrane research in a universally understandable mannerEmphasis on the historical development of the field. Topics include membrane sugars, membrane models, membrane isolation methods, and membrane transport.
  • Also available: Print – 2008
  • Chapter 1. Ultrasound, general considerations -- Chapter 2. The interventional cytopathologist -- Chapter 3. The thyroid gland -- Chapter 4. The parathyroid gland -- Chapter 5. The salivary glands -- Chapter 6. The head and neck, miscellaneous lesions -- Chapter 7. The lymph nodes -- Chapter 8. The breast -- Index.
  • Part 1. Cell polarity, growth, and cell cycle -- part 2. Yeast regulatory circuits -- part 3. Gene expression and trait locus analysis -- part 4. Comparative genomics.
  • Ion channel factsbook 1996
    1. Extracellular ligand-gated channels -- 2. Intracellular ligand-gated channels -- 3. Inward rectifier and intercellular channels -- 4. Voltage-gated channels.
  • The Continuing Fascination with Jaks and Stats: An Introduction / Thomas Decker and Mathias Müller -- Regulation of JAKs: Insights Gleaned from the Functional Protein Domains / Claude Haan, Daniela Ungureanu, Tuija Pekkala, Olli Silvennoinen and Serge Haan -- Nuclear Functions of the Janus Kinases / Mark A. Dawson and Andrew J. Bannister -- Self-association of STAT Proteins from Monomers to Paracrystals / Mathias Droescher and Uwe Vinkemeier -- How Stats Interact with the Molecular Machinery of Transcriptional Activation / Amanda M. Jamieson, Matthias Farlik and Thomas Decker -- Nongenomic Functions of STAT3 / Daniel J. Gough, Pravin Sehgal and David E. Levy -- Evolution of Jak and Stat Proteins / Claus Vogl, Priyank Shukla and Ingo Ebersberger -- JAK/STAT and Chromatin Regulation in Drosophila / Kriti Gaur and Willis X. Li -- JAK/STAT Signaling and Invertebrate Immune Responses / Feng Zhou and Hervé Agaisse -- Stat5 as a Hematopoietic Master Regulator for Differentiation and Neoplasia Development / Harini Nivarthi, Katrin Friedbichler and Richard Moriggl -- Regulation of Dendritic Cell Development by STATs / Haiyan S. Li and Stephanie S. Watowich. STAT Transcription Factors: Controlling All Aspects of NK Cell Biology / Eva Maria Putz, Eva Zebedin and Veronika Sexl -- Function of JAKs and STATs in Lymphocytes: Bench to Bedside / Alejandro Villarino, Xiang-Ping Yang, Kiyoshi Hirahara, Yuka Kanno and John J. O' Shea, et al. -- The Role of Janus Kinases in Hematopoietic Malignancies / Damla Olcaydu and Robert Kralovics -- Activation of Janus Kinases During Tumorigenesis / Jeffrey W. Schmidt and Kay-Uwe Wagner -- STAT1 and STAT3 Transcription Factors in Inflammation-associated Colon Cancer / Paulina Rampetsreiter and Robert Eferl -- Universal and Specific Functions of STAT3 in Solid Tumours / Lidia Avalle, Gabriella Regis and Valeria Poli -- Impact of STAT5 on Normal Tissue Development and Cancer / Maria M. Caffarel and Christine J. Watson -- Crosstalk with the Jak-STAT Pathway in Inflammation / Lionel B. Ivashkiv -- Activation and Inhibition of JAK-STAT Signal Transduction by RNA Viruses / Curt M. Horvath -- Multifaceted Antiviral Actions of Interferon-stimulated Gene Products / Birgit Strobl, Nicole R. Leitner and Mathias Müller -- Interferon Regulation of the Innate Response to Bacteria / Ali Abdul-Sater and Christian Schindler.
  • Analysis of Janus tyrosine kinase phosphorylation and activation / Jeremy A. Ross, Georgialina Rodriguez, and Robert A. Kirken -- Co-immunoprecipitation protocol to investigate cytokine receptor-associated proteins, e.g., Janus kinases or other associated signaling proteins / Claude Haan and Serge Haan -- In vitro JAK kinase activity and inhibition assays / Jeffrey J. Babon and James M. Murphy -- Quantitative analysis of JAK binding using isothermal titration calorimetry and surface plasmon resonance / Jeffrey J. Babon -- Determination of protein turnover rates in the JAK/STAT pathway using a radioactive pulse-chase approach / Anna Dittrich, Elmar Siewert, and Fred Schaper -- Designing RNAi screens to identify JAK/STAT pathway components / Katherine H. Fisher, Stephen Brown, and Martin P. Zeidler -- The use of JAK-specific inhibitors as chemical biology tools / Christopher J. Burns, David Segal, and Andrew F. Wilks -- Methods for detecting mutations in the human JAK2 gene / Anthony J. Bench, E. Joanna Baxter, and Anthony R. Green -- Detection of activated STAT proteins / Jean-Patrick Parisien and Curt M. Horvath -- Detection of activated STAT species using electrophoretic mobility shift assay (EMSA) and potential pitfalls arising from the use of detergents / Serge Haan and Claude Haan -- Flow cytometric analysis of STAT phosphorylation / Jane Murphy and Gabrielle L. Goldberg -- Acetylation of endogenous STAT proteins / Torsten Ginter, Thorsten Heinzel, and Oliver H. Krämer -- Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining / Reza Khorooshi and Trevor Owens -- Nuclear trafficking of STAT proteins visualized by live cell imaging / Velasco Cimica and Nancy C. Reich -- Characterization of STAT self-association by analytical ultracentrifugation / Nikola Wenta and Uwe Vinkemeier -- Constitutively active STAT5 constructs / Lynn M. Heltemes-Harris and Michael A. Farrar -- Analysis of suppressor of cytokine signalling (SOCS) gene expression by real-time quantitative PCR / Tatiana B. Kolesnik and Sandra E. Nicholson -- Detection of endogenous SOCS1 and SOCS3 proteins by immunoprecipitation and western blot analysis / Jian-Guo Zhang and Sandra E. Nicholson -- In vitro ubiquitination and cytokine signaling components / Jeffrey J. Babon, Artem Laktyushin, and Nadia J. Kershaw -- Production and crystallization of recombinant JAK proteins / Isabelle S. Lucet and Rebecca Bamert -- Bacterial expression, purification, and crystallization of tyrosine phosphorylated STAT proteins / Florence Baudin and Christoph W. Müller.
  • Centromeres and kinetochores : an historical perspective / Kerry S. Bloom -- The basics of chromosome segregation / Mitsuhiro Yanagida -- The centromere / Beth A. Sullivan -- Neocentromeres / Owen J. Marshall and K.H. Andy Choo -- Human artificial centromeres : de novo assembly of functional centromeres on human artificial chromosomes / Hiroshi Masumoto, Teruaki Okada, and Yasuhide Okamoto -- Kinetochore composition, formation, and organization / Tatsuo Fukagawa and Peter De Wulf -- Evolution of centromeres and kinetochores : a two-part fugue / Paul B. Talbert, Joshua J. Bayes, and Steven Henikoff -- Mitotic spindle assembly mechanisms / Rebecca Heald and Claire E. Walczak -- Kinetochore-microtubule interactions / Lesley Clayton and Tomoyuki U. Tanaka -- Post-translational modifications that regulate kinetochore activity / Chitra V. Kotwaliwale and Sue Biggins -- The role of the kinetochore in spindle checkpoint signaling / P. Todd Stukenberg and Daniel J. Burke -- Kinetochore regulation of anaphase and cytokinesis / Scott Thomas and Kenneth B. Kaplan -- Roles of centromeres and kinetochores in meiosis / Adele L. Marston -- The kinetochore-cancer connection / Takeshi Tomonaga -- The kinetochore as target for cancer drug development / Song-tao Liu and Tim J. Yen.
  • Also available: Print – 2013
  • Indication, Collection, and Laboratory Processing of Cytologic Samples -- The Cellular and Acellular Components of the Urinary Sediment -- The Cytologic Makeup of the Urinary Sediment According to the Collection Technique -- Cytologic Manifestations of Benign Disorders Affecting Cells of the Lower Urinary Tract -- Tumors and Related Conditions of the Bladder and Lower Urinary Tract -- Urine-Based Assays Complementing Cytologic Examination in the Detection of Urothelial Neoplasm.
  • Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields.
  • Structural dynamics of nucleic acids by single-molecule FRET / Asger Christian Krüger, Lasse Lava Hildebrandt, Sofie Louise Kragh, Victoria Birkedal -- Visualizing the location and the dynamics of gene expression in living animals through bioluminescence imaging / Xiaojuan Chen -- Quality control protocol for confocal system / Anda Cornea -- Electron microscopy of bone / V. Everts, K.A. Hoeben -- Immunostaining : detection of signaling protein location in tissues, cells and subcellular compartments / Biswanath Maity, David Sheff, Rory A. Fisher -- Bimolecular fluorescence complementation (BiFC) analysis of protein-protein interaction : how to calculate signal-to-noise ratio / Yutaka Kodama, Chang-Deng Hu -- Immunohistochemical detection of neuropeptides/transmitters in mammalian brain / Lothar Jennes -- Intravital imaging of autoreactive T. cells in living animals / Naoto Kawakami, Ingo Bartholomäus, Marija Pesic, Nikolaos I. Kyratsous -- Genetically encoded click chemistry for single-molecule FRET of proteins / Swati Tyagi, Edward A. Lemke -- High-throughput methods for the detection of protein overexpression using fluorescence markers / Mads Gabrielsen, Andrew J. Roe, Karen McLuskey -- Correlative microscopy / Alexander A. Mironov, Galina V. Beznoussenko -- Stereology of the lung / Jan Philipp Schneider, Matthias Ochs -- Quantitative 3D cell-based assay performed with cellular spheroids and fluorescence microscopy / Nariman Ansari, Stefanie Müller, Ernst H.K. Stelzer, Francesco Pampaloni -- Optimizing fluorescence excitation and detection for intravital two-photon microscopy / Dan Suan, Henry R. Hampton, Michio Tomura, Osami Kanagawa, Tatyana Chtanova, Tri Giang Phan -- Two-dimensional crystallization by dialysis for structural studies of membrane proteins by the cryo-EM method electron crystallography / Matthew C. Johnson, Ingeborg Schmidt-Krey -- In situ hybridization / Chung-Jui Tsai, Scott A. Harding -- RNA imaging : tracking in real-time RNA transport in neurons using molecular beacons and confocal microscopy / Angélica Zepeda, Clorinda Arias, Fabian Flores-Jasso, Luis Vaca.
  • "Lactoferrin was first identified as an iron-binding glycoprotein abundantly found in milk. It has been considered to play important roles in host defense, since lactoferrin shows broad spectrum of anti-bacterial and anti-viral activities, arising from its iron-chelating property. Furthermore, lactoferrin can regulate the function of innate and adaptive immune cells and exhibits immuno-modulating properties. These activities of lactoferrin implicate that potential use of lactoferrin as a wound healing agent. In addition, this book shows that lactoferrin is a potent regulator of dermal fibroblasts and epidermal keratinocytes, and promotes skin or corneal epithelial wound healing by increasing their proliferation, migration or deposition of extracellular matrix (ECM) components such as collagen and hyaluronan. This book provides the scientific background on use of lactoferrin as a wound healing agent. Indeed, lactoferrin exerts its biological effects by binding to specific lactoferrin receptors on target cells. This book also covers the in-depth information on roles of lactoferrin as a signaling molecule."--Publisher's website.
  • Also available: Print – 2007
  • This book is the first to summarize the current knowledge of the cell biology of lens epithelial cells in relation to and in the development of posterior capsular opacification (PCO). PCO remains the most common long-term complication of modern cataract surgery, occurring months or years after cataract surgery, unlike most other complications that tend to arise during or soon after the procedure. Opacification of the posterior capsule appears to be linked to lens epithelial cells that are left behind in the eye during cataract removal. These cells proliferate, migrate across the posterior lens capsule, and undergo changes that result in fibrous or pearl-type opacities in the capsule. The first section of the text explains the molecular mechanism and biology of lens epithelial cells that lead to the incidence of PCO. In the second part, in addition to a description of the mechanism and pathological condition of PCO, surgical methods and devices for preventing PCO are discussed in detail. Lens Epithelium and Capsular Opacification will benefit not only young clinical residents and junior researchers, but also established faculty in the clinical or basic academic field.
  • Glycol methacrylate embedding for improved morphological, morphometrical, and immunohistochemical investigations under light microscopy : testes as a model / Hélio Chiarini-Garcia, Gleydes Gambogi Parreira, and Fernanda R.C.L. Almeida -- Histological processing of teeth and periodontal tissues for light microscopy analysis / Gerluza Aparecida Borges Silva, Adriana Moreira, and José Bento Alves -- Large plant samples: how to process for GMA embedding / Élder Antônio Sousa Paiva, Sheila Zambello de Pinho, and Denise Maria Trombert Oliveira -- Image cytometry : nuclear and chromosomal DNA quantification / Carlos Roberto Carvalho, Wellington Ronildo Clarindo, and Isabella Santiago Abreu -- Histological approaches to study tissue parasitism during the experimental Trypanosoma cruzi infection / Daniela L. Fabrino, Grazielle A. Ribeiro, Livia Teixeira, and Rossana C.N. Melo -- Intravital microscopy to study leukocyte recruitment in vivo / Vanessa Pinho ... [et al.] -- Introduction to fluorescence microscopy / Ionita C. Ghiran -- Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals / Ernani Amaral, Silvia Guatimosim, and Cristina Guatimosim -- Imaging lipid bodies within leukocytes with different light microscopy techniques / Rossana C.N. Melo ... [et al.] -- Eicosa cell : an immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites / Christianne Bandeira-Melo, Peter F. Weller, and Patricia T. Bozza -- Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer / Robert M. Hoffman -- Imaging calcium sparks in cardiac myocytes / Silvia Guatimosim, Cristina Guatimosim, and Long-Sheng Song -- Light microscopy in aquatic ecology: methods for plankton communities studies / Maria Carolina S. Soares ... [et al.] -- Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedded samples / Shin-ichi Iwasaki and Hidekazu Aoyagi.
  • Lipid droplets 2013
    This new volume of Methods in Cell Biology looks at lipid droplets LDs, covering sections on analyses of LDs in model systems, cell/tissue-specific analyses of LDs and imaging and in vitro analyses of LD biogenesis and growth. Chapters are written by experts in the field. With cutting-edge material, this comprehensive collection is intended to guide researchers of LDs for years to come. Covers sections on analyses of lipid droplets (LDs) in model systems, cell/tissue-specific analyses of LDs and imaging, and in vitro analyses of LD biogenesis and growth Chapters are written by experts in t
  • Also available: Print – 2013
    Sphingomyelin and its role in cellular signaling / Mahua Chakraborty and Xian-Cheng Jiang -- The driving force of alpha-synuclein insertion and amyloid channel formation in the plasma membrane of neural cells: key role of ganglioside- and cholesterol-binding domains / Jacques Fantini and Nouara Yahi -- The enigmatic role of sulfatides: new insights into cellular functions and mechanisms of protein recognition / Shuyan Xiao, Carla V. Finkielstein, and Daniel G. S. Capelluto -- Phosphoinositides and PDZ domain scaffolds / Anna Maria Wawrzyniak, Rudra Kashyap and Pascale Zimmermann -- PtdIns(4)P signalling and recognition systems / Marc Lenoir and Michael Overduin -- PtdIns(4,5)P2-mediated cell signaling: emerging principles and PTEN as a paradigm for regulatory mechanism / Arne Gericke, Nicholas R. Leslie, Mathias Lösche, and Alonzo H. Ross -- Role of phosphatidylinositol 3,4,5-trisphosphate in cell signaling / Robert D Riehle, Sinziana Cornea and Alexei Degterev -- The role of phosphoinositides and inositol phosphates in plant cell signaling / Glenda E. Gillaspy -- Phosphatidic acid-mediated signaling / Yu Liu, Yuan Su, and Xuemin Wang -- Phosphatidylserine-mediated cellular signaling / Jason G. Kay and Sergio Grinstein -- Cardiolipin-mediated cellular signaling / Vinay A. Patil and Miriam L. Greenberg.
  • Lipids. 1st ed. 2012
    Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding / Min Wu, Pietro de Camilli -- Studying lipids involved in the endosomal pathway / Christin Bissig, Shem Johnson, Jean Gruenberg -- Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking / Jean-Baptiste Manneville, Cécile Leduc, Benoit Sorre, Guillaume Drin -- Reconstituting multivesicular body biogenesis with purified components / Thomas Wollert -- Approaches to the study of Atg8-mediated membrane dynamics in vitro / Anjai Jotwani, Diana N. Richerson, Isabelle Motta, Omar Julca-Zevallos, Thomas J. Melia -- Reconstitution assay system for ceramide transport with semi-intact cells / Keigo Kumagai, Masahiro Nishijima, Kentaro Hanada -- Visualizing mitochondrial lipids and fusion events in mammalian cells / Huiyan Huang, Michael A. Frohman -- Targeted and non-targeted analysis of membrane lipids using mass spectrometry / Xue Li Guan, Markus R. Wenk -- Modulation of host phosphoinositide metabolism during salmonella invasion by the type III secreted effector SopB / Bernhard Roppenser, Sergio Grinstein, John H. Brumell -- Acute manipulation of phosphoinositide levels in cells / Belle Chang-Ileto, Samual G. Frere, Gilbert Di Paolo -- Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins / Marnix Wieffer, Volker Haucke, Michael Krauß -- Phosphoinositides at the neuromuscular junction of drosophila melanogaster : a genetic approach / Jan R. Slabbaert, Thang Manh Khuong, Patrik Verstreken -- Devising powerful genetics, biochemical and structural tools in the functional analysis of phosphatidylinositol transfer proteins (PITPs) across diverse species / James M. Davison, Vytas A. Bankaitis, Ratna Ghosh -- Genome-wide screens for gene products regulating lipid droplet dynamics / Weihua Fei, Hongyuan Yang -- The three dimensionality of cell membranes : lamellar to cubic membrane transition as investigated by electron microsocopy / Ketpin Chong, Yuru Deng -- Quantitative imaging of lipid metabolism in yeast : from 4D analysis to high content screens of mutant libraries / Heimo Wolinski, Kristian Bredies, Sepp D. Kohlwein -- Analysis of cholesterol trafficking with fluorescent probes / Frederick R. Maxfield, Daniel Wüstner -- Fluorescence correlation methods for imaging cellular behavior of sphingolipid-interacting probes / Rachel Kraut, Nirmalya Bag, Thorsten Wohland -- Monitoring phospholipid dynamics during phagocytosis : application of genetically-encoded fluorescent probes / Helen Sarantis, Sergio Grinstein -- Genetically encoded probes for phosopatidic acid / Nawal Kassas, Petra Tryoen-Tóth, Matthias Corrotte, Tamou Thahouly, Marie-France Bader, Nancy J. Grant, Nicolas Vitale.
  • Also available: Print – 2006
    Protein kinase C-theta (PKC[theta]): new perspectives on its functions in T-cell biology / Rina Barouch-Bentov and Amnon Altman -- Travel informations on the tec kinases during lymphocyte activation / Fabien Garçon and Jacques A. Nunès -- Inducible T-cell tyrosine kinase (ITK): structural requirements and actin polymerization / Constantine D. Tsoukas ... [et al.] -- Differential requirements of PI3K subunits for BCR or BCR/CD19-induced ERK activation / Elena Vigorito and Martin Turner -- Protein tyrosine phosphatases in human disease / Tomas Mustelin -- The T-cell-specific adapter protein functions as a regulator of peripheral but not central immunological tolerance / Philip E. Lapinski ... [et al.] -- Proximal signals controlling B-cell antigen receptor (BCR) mediated NF-[kappa]B activation / Miguel E. Moreno-García ... [et al.] -- The adapter 3BP2: how it plugs into leukocyte signaling / Marcel Deckert and Robert Rottapel -- CTLA-4 regulation of T-cell function via RAP-1-mediated adhesion / Helga Schneider ... [et al.] -- Protein crosstalk in lipid rafts / Raquel J. Nunes, Mónica A.A. Castro, and Alexandre M. Carmo -- Role of lipid rafts in activation-induced cell death: the fas pathway in aging / Anis Larbi ... [et al.] -- T-cell response in aging: influence of cellular cholesterol modulation / Tamàs Fülöp ... [et al.] -- Lymphocyte signaling and the translatability of mRNA / Suzanne Miyamoto -- Protein arginine methylation: a new frontier in T-cell signal transduction / Brandon T. Schurter, Fabien Blanchet, and Oreste Acuto -- Immune regulation by ubiquitin conjugation / K. Venuprasad ... [et al.] -- CTIB (C-terminus protein of I[kappa]B-[beta]): a novel factor required for acidic adaptation /Toshiko Fukamachi ... [et al.] -- HIV may deplete most CD4 lymphocytes by a mechanism involving signaling through its receptors on non-permissive resting lymphocytes / Miles W. Cloyd ... [et al.] -- Integrating traditional and postgenomic approaches to investigate lymphocyte development and function / Yina Hsing Huang ... [et al.] -- Phosphoproteomic analysis of lymphocyte signaling / Lulu Cao, Kebing Yu, and Arthur R. Salomon.
  • Lysosomes 2005
    History and morphology of the lysosome / Renate Lüllmann-Rauch -- Transport of lysosomal enzymes / Stephan Storch and Thomas Braulke -- Adaptor proteins in lysosomal biogenesis / Peter Schu -- Lysosomal membrane proteins / Paul Saftig -- Lysosomal proteases: revival of the sleeping beauty / Klaudia Brix -- Lysosomal storage disorders / Ole Kristian Greiner-Tollersrud and Thomas Berg -- Lysosomal transporters and associated diseases / Frans W. Verheijen and Grazia M.S. Mancini -- Neuronal ceroid-lipofuscinoses / Jaana Tyynelè -- Cholesterol transport in lysosomes / Judith Storch and Sunita R. Cheruku -- Therapy of lysosomal storage diseases / Ulrich Matzner -- Lysosomal proteome and transcriptome / Jobst Landgrebe and Torben Lubke -- External lysosomes: the osteoclast and its unique capacities / Vincent Everts and Wouter Beertsen -- Membrane resealing mediated by lysosomal exocytosis / Norma W. Andrews -- Macroautophagy in mammalian cells / Eeva-Liisa Eskelinen -- Chaperone-mediated autophagy / Erwin Knecht and Natalia Salvador.
  • Introduction -- Molecular machines -- The processes of living -- Molecules in cells: Escherichia coli -- A human cell: The advantages of compartments -- The human body: The advantages of specialization -- Life and death -- Viruses -- You and your molecules.
  • Generation of retroviral macrophage cDNA expression libraries and functional screening for surface receptors / Georgia Schäfer and Gordon D. Brown -- Generation of murine growth factor-dependent long-term dendritic cell lines to investigate host-parasite interactions / Alessandra Mortellaro ... [et al.] -- Macrophage activation : classical vs. alternative / Andrea Classen, Jorge Lloberas, and Antonio Celada -- Analysis of macrophage phagocytosis : quantitative assays of phagosome formation and maturation using high-throughput florescence microscopy / Benjamin E. Steinberg and Sergio Grinstein -- Detection of activated Rab7 GTPase with an immobilized RILP probe / Jim Sun ... [et al.] -- Probing in vivo origins of mononuclear phagocytes by conditional ablation and reconstitution / Chen Varol, Limor Landsman, and Steffen Jung -- Multivalent protein probes for the identification and characterization of cognate cellular ligands for myeloid cell surface receptors / Hsi-Hsien Lin ... [et al.] -- Exploiting Fc chimaeric proteins for the identification of ligands specific for the mannose receptor / Luisa Martinez-Pomares -- The expression of exogenous genes in macrophages : obstacles and opportunities / Xia Zhang, Justin P. Edwards, and David M. Mosser -- Genetic modification of murine dendritic cells by RNA transfection / Maurizio Ceppi, Enrico Schmidt, and Philippe Pierre -- Recording phagosome maturation through the real-time, spectrofluorometric measurement of hydrolytic activities / Robin M. Yates, Albin Hermetter, and David G. Russell -- Derivation and characterization of murine alternatively activated (M2) macrophages / Victor W.H. Ho and Laura M. Sly -- Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology / Mattias Svensson -- Generation, culture, and flow-cytometric characterization of primary mouse macrophages / Ulrike Schleicher and Christian Bogdan -- Analyzing classical and alternative macrophage activation in macrophage/neutrophil-specific IL-4 receptor-alpha-deficient mice / Frank Brombacher ... [et al.] -- Analysis of matrix metalloproteinase secretion by macrophages / Paul T. Elkington, Justin A. Green, and Jon S. Friedland -- Identification of pathogen receptors on dendritic cells to understand their function and to identify new drug targets / Lot de Witte ... [et al.] -- Stable lentiviral vector-mediated gene silencing in human monocytic cell lines / Jimmy S. Lee and Neil E. Reiner -- Use of fluorescent probes to detect lipid signaling intermediates in macrophages / Vivan Kwok, Eric Vachon, and Gregory P. Downey -- Large-scale phagosome preparation / Adrien F. Vinet and Albert Descoteaux -- Coordinate regulation of sphingosine kinase and actin dynamics / Shankar S. Iyer and David J. Kusner.
  • Magnetic cell separation 2008
    Also available: Print – 2008
  • Scientists investigating germ cells have, over the past 15 years, originated discoveries and innovations that give us valuable insights into the mechanisms that regulate not just stem cell function, but human development in its widest sense. With contributions from some of the leading researchers in the field, Male Germline Stem Cells: Developmental and Regenerative Potential assesses the implications of these discoveries for understanding the fundamental biology of germline stem cells as well as their potential for human stem cell-based therapies. This monograph covers many of the fundamental issues now being explored by today's generation of stem cell researchers, including the field's potential for regenerative medicine.
  • Studying recombination in mouse oocytes / Xianfei Sun and Paula E. Cohen -- Analysis of recombinants in female mouse meiosis / Esther de Boer, Maria Jasin, and Scott Keeney -- Studying meiosis-specific cohesins in mouse embryonic oocytes / Ji-hye Kim [and others] -- Immunohistochemical approaches to the study of human fetal ovarian development / Jing He [and others] -- Protein kinase assays for measuring MPF and MAPK activities in mouse and rat oocytes and early embryos / Jacek Z. Kubiak -- Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis I in mouse oocytes / Janet E. Holt, Simon I.R. Lane, and Keith T. Jones -- Using FRET to study ranGTP gradients in live mouse oocytes / Julien Dumont and Marie-Hélène Verlhac -- Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes / Mark Levasseur -- RNAi-based methods for gene silencing in mouse oocytes / Paula Stein, Petr Svoboda, and Richard M. Schultz -- Micro-injection of morpholino oligonucleotides for depleting securin in mouse oocytes / Petros Marangos -- Measuring transport and accumulation of radiolabeled substrates in oocytes and embryos / Jay M. Baltz, Hannah E. Corbett, and Samantha Richard -- Immunofluorescence staining of spindles, chromosomes, and kinetochores in human oocytes / Solon Riris [and others] -- Studying the roles of aurora-C kinase during meiosis in mouse oocytes / Kuo-Tai Yang [and others] -- Chromosome spreads with centromere staining in mouse oocytes / Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann -- Preparation of mammalian oocytes for transmission electron microscopy / Lynne Anguish and Scott Coonrod -- Measuring Ca² oscillations in mammalian eggs / Karl Swann -- Counting chromosomes in intact eggs / Teresa Chiang and Michael A. Lampson -- Free-hand bisection of mouse oocytes and embryos / Zbigniew Polanski and Jacek Z. Kubiak -- Microarray-CGH for the assessment of aneuploidy in human polar bodies and oocytes / Souraya Jaroudi and Dagan Wells -- Nuclear transfer in the mouse oocyte / Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama.
  • Also available: Print – 2011
    Mast cell biology : introduction and overview / Alasdair M. Gilfillan, Sarah J. Austin, and Dean D. Metcalfe -- Mast cell progenitor trafficking and maturation / Jenny Hallgren and Michael F. Gurish -- Monomeric IgE and mast cell development, survival, and function / Jun-ichi Kashiwakura, Iris M. Otani, and Toshiaki Kawakami -- Mast cell apoptosis and survival / Maria Ekoff and Gunnar Nilsson -- Regulators of Ca2+ signaling in mast cells: Potential targets for treatment of mast cell-related diseases / Hong-Tao Ma and Michael A. Beaven -- Spatio-temporal signaling in mast cells / Bridget S. Wilson, Janet M. Oliver, and Diane S. Lidke -- The mechanisms of exocytosis in mast cells / Ulrich Blank -- An emerging role for the lipid mediator sphingosine-1-phosphate in mast cell effector function and allergic disease / Ana Olivera and Juan Rivera -- Down-regulation of mast cell responses through itim containing inhibitory receptors / Laila Karra and Francesca Levi-Schaffer -- The mast cell in innate and adaptive immunity / Christopher P. Shelburne and Soman N. Abraham -- Mast cells and immunoregulation/immunomodulation / Mindy Tsai, Michele Grimbaldeston, and Stephen J. Galli -- Mast cell proteases as protective and inflammatory mediators / George H. Caughey -- Mast cells in lung inflammation / Elena P. Moiseeva and Peter Bradding -- Mast cells, angiogenesis, and cancer / Domenico Ribatti and Enrico Crivellato.
  • Mast cells : methods and protocols 2005
    Springer Protocols
    Paul Ehrlich's "Mastzellen"--from aniline dyes to DNA chip arrays : a historical review of developments in mast cell research / Harsha Vyas and Guha Krishnaswamy -- The human mast cell : an overview / Guha Krishnaswamy, Omar Ajitawi, and David S. Chi -- Mast cells in allergy and autoimmunity : implications for adaptive immunity / Gregory D. Gregory and Melissa A. Brown -- Analysis of MCT and MCTC mast cells in tissue / Lawrence B. Schwartz -- Mast cell ultrastructure and staining in tissue / Shruti A. Shukla ... [et al.] -- Expression of cell surface antigens on mast cells : mast cell phenotyping / Alexander W. Hauswirth ... [et al.] -- Identification of mast cells in the cellular response to myocardial infarction / Nikolaos G. Frangogiannis and Mark L. Entman -- Growth of human mast cells from bone marrow and peripheral blood-derived CD34+ pluripotent progenitor cells / Arnold S. Kirshenbaum and Dean D. Metcalfe -- Culture of human mast cells from hemopoietic progenitors / Hirohisa Saito -- Isolation, culture, and characterization of intestinal mast cells / Gernot Sellge and Stephan C. Bischoff -- Activation of nuclear factor kappa B / Chuanfu Li, Jim Kelley, and Tuanzhu Ha -- Analysis of mitogen-activated protein kinase activation / Stephen C. Armstrong -- Microarray and gene clustering analysis / Tsung-Hsien Tsai, Denise M. Milhorn, and Shau-Ku Huang -- Techniques to study Fc [epsilon] RI signaling / Yuko Kawakami, Jiro Kitaura, and Toshiaki Kawakami -- Human mast cell proteases : activity assays using thiobenzyl ester substrates / David A. Johnson -- Mast cell histamine and cytokine assays / David S. Chi, S. Matthew Fitzgerald, and Guha Krishnaswamy -- Measurement of mast cell cytokine release by multiplex assay / Kevin F. Breuel and W. Keith De Ponti -- Assays for histamine-releasing factors : from identification and cloning to discovery of binding partners / Jacqueline M. Langdon and Susan M. McDonald -- Assays for nitric oxide expression / William L. Stone, Hongsong Yang, and Min Qui -- Immunohistological detection of growth factors and cytokines in tissue mast cells / Zhenhong Qu -- Endothelial cell activation by mast cell mediators / Kottarappat N. Dileepan and Daniel J. Stechschulte -- Co-culture of mast cells with fibroblasts : a tool to study their crosstalk / Ido Bachelet, Ariel Munitz, and Francesca Levi-Schaffer -- Detection of E class switching and IgE synthesis in human B cells / Jérôme Péne ... [et al.] -- Gene silencing using small interference RNA in mast cells / Deling Yin and Charles A. Stuart -- Mast cell activation by lipoproteins / Jim Kelley ... [et al.] -- Mast cell activation by stress / Ann L. Baldwin -- In vivo models for studying mast cell-dependent responses to bacterial infection / Christopher P. Shelburne, James B. McLachlan, and Soman N. Abraham -- Bacterial activation of mast cells / David S. Chi ... [et al.] -- Activation of mast cells by streptolysin O and lipopolysaccharide / Michael Stassen ... [et al.] -- Mast cell apoptosis / Alexander Gerbaulet, Karin Hartmann, and Yoseph A. Mekori.
  • Mast cells and basophils 2000
  • Preface -- Thymocyte development -- A review of mathematical models for T cell receptor triggering and antigen discrimination -- Dynamic tuning of T cell receptor specificity by co-receptors and costimulation -- T cell activation and function: role of signal strength -- The cyton model for lymphocyte proliferation and differentiation -- Modeling itravital two-photon data of lymphocyte migration and interaction -- Modeling lymphocyte dynamics in vivo -- Continuous-time birth and death processes: diversity maintenance of naïve T cells in the periphery -- Multivariate competition processes: a model for two competing T cell clonotypes -- Stochastic modeling of T Cell homeostasis for two competing clonotypes via the master equation -- Dendritic cell migration in the intestinal tract -- Reassessing germinal center reaction concepts -- B cell strategies of Ag recognition in a stratified immune system -- Dynamics of Peripheral regulatory and effector T cells competing for antigen presenting cells -- Mathematical models of the role of IL-2 in the interactions between helper and regulstory CD4+ T cells -- A Physicist{u2019}s approach to immunology -- Timescales of the adaptive immune response -- Using mathematical models to explore the role of cytoxic T lymphocytes in HIV infection -- Viral immunity and persistence -- Index.
  • The volume dwells on the major issues of mechanical stress influencing the ion channels and intracellular signaling pathways. This book is a unique collection of reviews outlining current knowledge and future developments in this rapidly growing field. In our opinion the book presents not only the latest achievements in the field but also brings the problem closer to the experts in related medical and biological sciences as well as practicing doctors. Knowledge of the mechanisms which underlie these processes is necessary for understanding of the normal functioning of different living organs and tissues and allows to predict changes, which arise due to alterations of their environment, and possibly will allow to develop new methods of artificial intervention. We also hope that presenting the problem will attract more attention to it both from researchers and practitioners and will assist to efficiently introduce it into the practical medicine.
  • Mechanosensitivity of cells from various tissues -- Mechanotransduction in the Nematode Caenorhabditis elegans -- Mechanically mediated crosstalk in heart -- Swelling-and stretch-activated chloride channels in the heart: regulation and function -- Role of stretch-activated channels in the heart: action potential and Ca2+ transients -- Isolated cardiomyocytes: mechanosensitivity of action potential, membrane current and ion concentration -- Mechano-electric feedback in the heart: evidence from intracellular microelectrode recordings on multicellular preparations and single cells from healthy and diseased tissue -- The Role of mechanosensitive fibroblasts in the heart -- Mechanical modulation of intracellular ion concentrations: mechanisms and electrical consequences -- Stretch-induced slow force response in mammalian ventricular myocardium -- The Na+/H+ exchanger as the main protagonist following myocardial stretch: the Anrep effect and myocardial hypertrophy -- Mechano-electric feedback and atrial arrhythmias -- The Role of mechano-electrical feedback in the cholinergic atrial fibrillation initiation --; Mechanosensitive cation channels of leech neurons -- Mechanosensitivity of primary afferent nociceptors in the pain pathway -- Stimulus-secretion coupling in the osmoreceptive prolactin cell of the tilapia -- Stretch-inactivated channels in skeletal muscle -- Stretch-activated cation channels and the myogenic response of small arteries -- Mechanobiology of bone tissue and bone cells -- Functional roles of mechanosensitive ion channels, ss1 integrins and kinase cascades in chondrocyte mechanotransduction -- Enigmatic roles of the epithelial sodium channel (ENaC) in articular chondrocytes and osteoblasts: mechanotransduction, sodium transport or extracellular sodium sensing?
  • Print Material
  • Also available: Print – 2012
    Medical Physiology, in its updated 2nd edition, firmly relates molecular and cellular biology to the study of human physiology and disease. Drs. Walter Boron and Emile Boulpaep and a team of leading physiologists present practical, accurate coverage emphasizing the clinical implications of the material. Each chapter explains the principles and organization of each body system, while more than 1400 high-quality, full-color line drawings and prominently featured clinical examples clarify every concept. This exceptionally detailed and comprehensive guide to physiology is ideal for a rich, straightforward, state-of-the-art understanding of this essential subject.
  • Quantitative analysis of cellular lipids by nano- electrospray ionization mass spectrometry / Cagakan Özbalci, Timo Sachsenheimer, and Britta Brügger -- Thin-layer chromatography of phospholipids / Rania M. Deranieh, Amit S. Joshi, and Miriam L. Greenberg -- Analysis of membrane lipid biogenesis pathways using yeast genetics / Martina Gsell and Günther Daum -- Using 2D crystals to analyze the structure of membrane proteins / Ian Collinson, Janet Vonck, and Dilem Hizlan -- Crystallization of membrane proteins / Florian G. Müller and C. Roy D. Lancaster -- Molecular dynamics simulations of membrane proteins / Kristyna Pluhackova, Tsjerk A. Wassenaar, and Rainer A. Böckmann -- Site-specific fluorescent probe labeling of mitochondrial membrane proteins / Christine T. Schwall and Nathan N. Alder -- Topology determination of untagged membrane proteins / Iris Nasie, Sonia Steiner-Mordoch, and Shimon Schuldiner -- self-assembling GFP : a versatile tool for plant (membrane) protein analyses / Katharina Wiesemann ... [et al.] -- Use of cardiolipin-containing liposomes as a model system to study the interaction between proteins and the inner mitochondrial membrane / Milit Marom and Abdussalam Azem -- Analysis of the interaction between membrane proteins and soluble binding partners by surface plasmon resonance / Zht Cheng Wu... [et al.] -- Peptide interaction with and insertion into membranes / Ron Saar-Dover, Avraham Ashkenazi, and Yechiel Shai -- Scanning fluorescence correlation spectroscopy in model membrane systems / Joseph D. Unsay and Ana J. García-Sáez -- Analyses of protein-protein Interactions by in vivo photocrosslinking in budding yeast / Takuya Shiota, Shuh-ichi Nishikawa, and Toshiya Endo -- Sedimentation velocity analytical ultracentrifugation in hydrogenated and deuterated solvents for the characterization of membrane proteins / Aline Le Roy ... [et al.] -- Membrane partitioning and translocation studied by isothermal titration calorimetry / Carolyn Vargas, Johannes Klingler, and Sandro Keller -- Analyzing membrane dynamics with live cell fluorescence microscopy with a focus on yeast mitochondria / Dirk Scholz ... [et al.] -- Analysis of protein translocation into the endoplasmic reticulum of human cells / Johanna Dudek ... [et al.] -- Assay to monitor the membrane integration of single-span proteins / Katrin Krumpe and Doron Rapaport -- Methods to study the biogenesis of membrane proteins in yeast mitochondria / Daniel Weckbecker and Johannes M. Herrmann -- Reconstitution of mitochondrial presequence translocase into proteoliposomes / Martin van der Laan, Ralf M. Zerbes, and Chris van der Does -- Single channel analysis of membrane proteins in artificial bilayer membranes / Philipp Bartsch, Anke Harsman, and Richard Wagner -- Quantification of protein complexes by blue native electrophoresis / Juliana Heidler ... [et al.] -- Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions / Anna Hjelm ... [et al.] .
  • From intramolecular asymmetries to raft assemblies: a short guide for the puzzled in lipidomics / Ephraim Yavin and Annette Brand -- Regulatory aspects of membrane microdomain (raft) dynamics in live cells: a biophysical approach / János Matkó and János Szöllosi -- Lipid raft membrane skeletons / Elizabeth J. Luna ... [et al.] -- Role of cholesterol in membrane microdomain signaling / Christopher J. Fielding -- Raft lipid metabolism in relation to alkyl-lysophospholipid-induced apoptosis / Arnold H. van der Luit, Marcel Verheij, and Wim J. van Blitterswijk -- Role of lipid rafts in signal transduction and synaptic plasticity of neural cells / Markus Delling and Melitta Schachner -- Role of rafts in virus fusion and budding / Wu Ou and Jonathan Silver -- Alterations in raft lipid metabolism in aging and neurodegenerative disorders / Mark P. Mattson, Roy G. Cutler, and Norman J. Haughey -- Caveolin and cancer: a complex relationship / Mordechai Liscovitch ... [et al.] -- Dietary modulation of lipid rafts: implications for disease prevention and treatment / Mark P. Mattson.
  • Membrane trafficking 2008
    Springer Protocols
    Pt. I. Yeast Cells -- Ch. 1. Freight management in the cell / Johannes M. Herrmann, Anne Spang -- Ch. 2. High-throughput protein extraction and immunoblotting analysis in saccharomyces cerevisiae / Todd C. Lorenz, Vikram C. Anand, Gregory S. Payne -- Ch. 3. Genome-wide analysis of membrane transport using yeast knockout arrays / Helen E. Burston, Michael Davey, Elizabeth Conibear -- Ch. 4. A cell-free system for reconstitution of transport between prevacuolar compartments and vacuoles in saccharomyces cerevisiae / Thomas A. Vida -- Ch. 5. In vitro analysis of the mitochondrial preprotein import machinery using recombinant precursor polypeptides / Dorothea Becker, Martin Krayl, Wolfgang Voos -- Ch. 6. In vitro import of proteins into isolated mitochondria / Karl Bihlmaier, Melanie Bien, Johannes M. Herrmann -- Ch. 7. Synthesis and sorting of mitochondrial translation products / Heike Bauerschmitt, Soledad Funes, Johannes M. Herrmann -- Ch. 8. In vivo labeling and analysis of mitochondrial translation products in budding and in fission yeasts / Karine Gouget, Fulvia Verde, Antoni Barrientos -- Ch. 9. Exploring protein-protein interactions involving newly synthesized mitochondrial DNA encoded proteins / Darryl Horn, Flavia Fontanesi, Antoni Barrientos -- Ch. 10. Purification of yeast membranes and organelles by sucrose density gradient centrifugation / Jennifer Chang, Victoria Ruiz, Ales Vancura -- Ch. 11. Microscopic analysis of lipid droplet metabolism and dynamics in yeast / Heimo Wolinski, Sepp D. Kohlwein -- Ch. 12. Use of bimolecular fluorescence complementation (BiFC) in yeast saccharomyces cerevisiae / Kari-Pekka Skarp, ... [et al.] -- Pt. II. Mammalian cells -- Ch. 13. Using quantitative fluorescence microscopy to probe organelle assembly and membrane trafficking / Brian Storrie, Tregei Starr, Kimberly Forsten-Williams -- Ch. 14. Measuring secretory membrane traffic / Vytaute Starkuviene, ... [et al.] -- Ch. 15. A correlative light and electron microscopy method based on laser micropatterning and etching / Julien Colombelli, ... [et al.] -- Ch. 16. Approaches to investigate the role of signaling in ER-to-Golgi transport / Laura J. Sharpe, Ximing Du, Andrew J. Brown -- Ch. 17. Recruitment of coat proteins to peptidoliposomes / Gregor Suri, Martin Spiess, Pascal Crottet -- Ch. 18. SNAREs mediated fusion of liposomes / Jérôme Vicogne, Jeffrey E. Pessin -- Ch. 19. Use of polarized PC12 cells to monitor protein localization in the early biosynthetic pathway / Ragna Sannerud, ... [et al.] -- Ch. 20. Tracking the transport of E-cadherin to and from the plasma membrane / Matthew P. Wagoner, Kun Ling, Richard A. Anderson -- Ch. 21. Analysis of nucleocytoplasmic shuttling of NFkB proteins in human leukocytes / Chandra C. Ghosh ... [et al.] -- Ch. 22. Imaging pHluorin-based probes at hippocampal synapses / Stephen J. Royle, ... [et al.] -- Ch. 23. Analysis of receptor tyrosine kinase internalization using flow cytometry / Ning Li, Kristen S. Hill, Lisa A. Elferink -- Ch. 24. Measurement of receptor endocytosis and recycling / Jane M. Knisely, Jiyeon Lee, Guojun Bu -- Ch. 25. Styryl dye-based synaptic vesicle recycling assay in cultured cerebellar granule neurons / Victor Anggono, Michael A. Cousin, Phillip J. Robinson -- Ch. 26. Use of quantitative immunofluorescence microscopy to study intracellular trafficking / Vincent Blot, Timothy E. McGraw -- Ch. 27. Dissecting GLUT4 traffic components in L6 myocytes by fluorescence-based, single cell assays / Costin N. Antonescu, Varinder K Randhawa, Amira Klip -- Ch. 28. Functional genetic analysis of the mammalian mitochondrial DNA encoded peptides / Maria Pilar Bayona-Bafaluy, ... [et al.].
  • Mesenchymal Stem Cells or Multipotent Mesenchymal Stromal Cells (collectively referred to as MSCs) are currently being tested in a number of clinical trials for various diseases including graft-versus-host disease, Crohn's disease, myocardial infarction, stroke, diabetes, cartilage defects, and many others. The increasing number of clinical trials globally indicates the likely therapeutic potential of this specialized adult stem cell. Over the last decade, there has been tremendous progress towards understanding an MSC's phenotype, expansion features in culture, differentiation ability and global gene and protein expression profiles. Despite the enormous progress and data accrual at the molecular and cellular levels, several questions remain as to the utility of MSCs for clinical applications. Mesenchymal Stem Cell Therapy is a comprehensive collection of information comprised of both basic and pre-clinical biological studies as well as current advances in clinical applications within the MSC therapy field. This text is a resource for both the basic life science and cell therapy researchers and includes a spectrum of review chapters from top experts in the field discussing clinical scale culture, regulatory issues, genetic engineering, disease specific applications and others.
  • Part I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.]. Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger. A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C. W. Zannettino. Adipose-derived stem cells / John K. Fraser ... [et al.]. Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois ... [et al.] -- Part II. Manipulation of human multipotential stromal cells. Colony forming unit assays for MSCs / Radhika Pochampally. Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe. Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe. Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi. Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prockop. Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- Part III. Isolation and characterization of murine multipotential stromal cells from bone marrow. Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney.
  • Also available: Print – 2002
  • Methods in cilia & flagella 2015
    Imaging centrosomes and cilia in the mouse kidney / Masato Hoshi, Jinzhi Wang, Sanjay Jain, Moe R. Mahjoub -- Ependymal cell differentiation, from monociliated to multiciliated cells / Nathalie Delgehyr, Alice Meunier, Marion Faucourt, Montserrat Bosch Grau, Laetitia Strehl, Carsten Janke, Nathalie Spassky -- Observing planar cell polarity in multiciliated mouse airway epithelial cells / Eszter K. Vladar, Yin Loon Lee, Tim Stearns, Jeffrey D. Axelrod -- Imaging and analyzing primary cilia in cardiac cells / Nicolas Diguet, Jean-Francois Le Garrec, Tommaso Lucchesi, Sigolene M. Meilhac -- Cilia in photoreceptors / Linjing Li, Manisha Anand, Kollu Nageswara Rao, Hemant Khanna -- Analysis of primary cilia in the developing mouse brain / Judith T.M.L. Paridaen, Wieland B. Huttner, Michaela Wilsch-Brauninger -- In vivo investigation of cilia structure and function using Xenopus / Eric R. Brooks, John B. Wallingford -- A quantitative approach to study endothelial cilia bending stiffness during blood flow mechanodetection in-vivo / Francesco Boselli, Jacky G. Goetz, Gilles Charvin, Julien Vermot -- Quantitative description of fluid flows produced by left-right cilia in zebrafish / Craig Fox, M. Lisa Manning, Jeffrey D. Amack -- Efficient live fluorescence imaging of intraflagellar transport in mammalian primary cilia / Hiroaki Ishikawa, Wallace F. Marshall -- Analysis of soluble protein entry into primary cilia using semipermeabilized cells / David K. Breslow, Maxence V. Nachury -- Methods for imaging individual cilia in living echinoid embryos / Robert L. Morris, Hans W. Pope, Adam N. Sholi, Leah M. Williams, Chelsea R. Ettinger, Gwendolyn M. Beacham, Tatsushi Shintaku, Zachary D. Abbott, Elyse M. Doherty -- The planarian Schmidtea mediterranea as a model for studying motile cilia and multiciliated cells / Cyril Basquin, Anne-Marie Orfila, Juliette Azimzadeh -- Quantitative analysis of flagellar proteins in Drosophila sperm tails / Teresa Mendes Maia, Perrine Paul-Gilloteaux, Renata Basto -- Imaging cilia in Drosophila melanogaster / Jennifer Vieillard, Jean-Luc Duteyrat, Elisabeth Cortier, Benedicte Durand -- Studying G protein-coupled receptors: Immunoblotting, immunoprecipitation, phosphorylation, surface labeling, and cross-linking protocols / Kasturi Pal, Hemant Badgandi, Saikat Mukhopadhyay -- Image analysis of Caenorhabditis elegans ciliary transition zone structure, ultrastructure, molecular composition and function / Anna A. W. M. Sanders, Julie Kennedy, Oliver E. Blacque -- Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii / Huawen Lin, Susan K. Dutcher -- Axonemal motility in Chlamydomonas / Ken-ichi Wakabayashi, Ritsu Kamiya -- Posttranscriptional control over rapid development and ciliogenesis in marsilea / Stephen M. Wolniak, Thomas C. Boothby, Corine M. van der Weele -- Total internal reflection fluorescence microscopy of intraflagellar transport in tetrahymena thermophila / Yu-Yang Jiang, Karl Lechtreck, Jacek Gaertig -- Ciliary heterogeneity within a single cell : the paramecium model / Anne Aubusson-Fleury, Jean Cohen, Michel Lemullois -- Imaging intraflagellar transport in trypanosomes / Julien Santi-Rocca, Nicolas Chenouard, Cécile Fort, Thibault Lagache, Jean-Christophe Olivo-Marin, Philippe Bastin -- Scanning and three-dimensional electron microscopy methods for the study of trypanosoma brucei and leishmania mexicana flagella / Eva Gluenz, Richard John Wheeler, Louise Hughes, Sue Vaughan.
  • Also available: Print – 2008
  • Expression of exogenous mRNA in Xenopus laevis embryos for the study of cell cycle regulation / Jill C. Sible and Brian N. Wroble -- Use of luciferase chimaera to monitor PLC[zeta] expression in mouse eggs / Karl Swann ... [et al.] -- Analysis of 14-3-3 family member function in Xenopus embryos by microinjection of antisense morpholino oligos / Jeffrey M.S. Lau and Anthony J. Muslin -- A microinjectable biological system, the Xenopus oocyte, as an approach to understanding signal transduction protein function / Katia Cailliau and Edith Browaeys-Poly -- Combining microinjection and immunoblotting to analyse MAP kinase phosphorylation in single starfish oocytes and eggs / David J. Carroll and Wei Hua -- Analysis of signaling pathways in zebrafish development by microinjection / William H. Kinsey -- Protein inhibition by microinjection and RNA-mediated interference in tissue culture cells : complementary approaches to study protein function / Jane R. Stout, Rania S. Rizk, and Claire E. Walczak -- DNA delivery by microinjection for the generation of recombinant mammalian cell lines / Sebastien Chenuet ... [et al.] -- Bacteriophage [phi]C31 integrase mediated transgenesis in Xenopus laevis for protein expression at endogenous levels / Bryan G. Allen and Daniel L. Weeks -- Germline transformation of Caenorhabditis elegans by injection / Pavan Kadandale, Indrani Chatterjee, and Andrew Singson -- Quantitative microinjection of mouse oocytes and eggs / Douglas Kline -- Microinjection of follicle-enclosed mouse oocytes / Laurinda A. Jaffe ... [et al.] -- Functional studies of regulatory genes in the sea urchin embryo / Vincenzo Cavalieri, Maria Di Bernardo, and Giovanni Spinelli -- Exploring the cytoskeleton during intracytoplasmic sperm injection in humans / Vanesa Y. Rawe and Héctor Chemes -- Somatic cell nuclear transfer in the mouse / Satoshi Kishigami and Teruhiko Wakayama.
  • Pt. A ScienceDirect
    Pt. B ScienceDirect
    Pt. C ScienceDirect
    "Micropatterning refers generally to techniques which provide an experimental control over the chemical, physical, or geometrical properties of materials at the micron or submicron scale, and are thus used to produce spatial patterns of these properties. These techniques, which were often originally designed for application in microelectronics, have spread over most areas of science, including biology. They have proved particularly useful for cell biology, bridging the gap between the Petri-dish and complex 3D assays and tissues."--Preface.
  • Also available: Print – 2015
    The book serves as a comprehensive resource for scientists and clinicians studying the role of non-coding RNAs in inflammation (viral infections, wound inflammation), human inflammatory diseases (i.e. rheumatoid arthritis, Crohnℓ́ℓs disease, diabetes), and innate immunity. It provides a universal reference work comprising both basic and specialized information. Given that ncRNAs represent new therapeutic targets, this volume will also be of interest to industrial biomedical researchers and those involved in drug development.
  • In situ detection of microRNAs in animals / Dylan Sweetman -- Detection of microRNAs in plants by in situ hybridisation / Eva Varallyay and Zoltan Havelda -- Detecting sRNAs by northern blotting / Sara Lopez-Gomollon -- Profiling microRNAs by real-time PCR / Nanna Jacobsen, Ditte Andreasen, and Peter Mouritzen -- Detection of small RNAs and microRNAs using deep sequencing technology / Ericka R. Havecker -- Detection of microRNAs in prostate cancer cells by mircoRNA array / Xiaoqing Tang [and others] -- MicroRNA knock down by cholesterol-conjugated antisence oligos in mouse organ culture / Sharon Kredo-Russo and Eran Hornstein -- Protocols for use of homologous recombination gene targeting to produce microRNA mutants in drosophila / Ya-Wen Chen, Ruifen Weng, and Stephen M. Cohen -- Engineering elements for gene silencing : the artificial microRNAs technology / Pablo Andres Manavella and Ignacio Rubio-Somoza -- Mimicry technology : suppressing small RNA activity in plants / Ignacio Rubio-Somoza and Pablo Andres Manavella -- Experimental validation of microRNA targets using a luciferase reporter system / Francisco E. Nicolas -- Experimental identification of microRNA targets by immunoprecipitation of argonaute protein complexes / Michaela Beitzinger and Gunter Meister -- Comprehensive identification of miRNA target sites in live animals / Dimitrios G. Zisoulis, Gene W. Yeo, and Amy E. Pasquinelli -- Target validation of plant microRNAs / Cesar Llave [and others] -- High-throughput sequencing-based methodology to identify all uncapped and cleaved RNA molecules in eukaryotic genomes / Matthew W. Endres, Rebecca T. Cook, and Brian D. Gregory.
  • Site-specific fluorescent labeling of tubulin / Kamalika Muhkerjee, Susan L. Bane -- Measuring microtubule persistence length using a microtubule gliding assay / Douglas S. Martin -- Structural studies of the doublecortin family of MAPs / Frank Fourniol, Mylène Perderiset, Anne Houdusse, Carolyn Moores -- Detection and quantification of microtubule detachment from centrosomes and spindle poles / Anutosh Ganguly, Hailing Yang, Fernando Cabral -- Regulation of tubulin expression by micro-RNAs : implications for drug resistance / Sharon Lobert, Mary E. Graichen -- Determining the structure-mechanics relationships of dense microtubule networks with confocal microscopy and magnetic tweezers-based microrheology / Yali Yang, Megan T. Valentine -- Studying mitochondria and microtubule localization and dynamics in standardized cell shapes / Andrea Pelikan, James Sillibourne, Stephanie Miserey-Lenkei, Frederique Carlier-Grynkorn, Bruno Goud, Phong T. Tran -- Going solo : measuring the motions of microtubules with an in vitro assay for TIRF microscopy / Kris Leslie, Niels Galjart -- Analysis of microtubules in isolated axoplasm from the squid giant axon / Yuyu Song, Scott T. Brady -- Imaging GTP-bound tubulin : from cellular to in vitro assembled microtubules / Hélène de Forges, Antoine Pilon, Christian Poüs, Franck Perez -- Tubulin-specific chaperones : components of a molecular machine that assembles the ... heterodimer / Guoling Tian, Nicholas J. Cowan -- Heterotrimeric G proteins and microtubules / Witchuda Saengsawang, Mark M. Rasenick -- Purification and biophysical analysis of microtubule-severing enzymes in vitro / Juan Daniel Diaz-Valencia, Megan Bailey, Jennifer L. Ross -- Measurement of in vitro microtubule polymerization by turbidity and fluorescence / Matthew Mirigian, Kamalika Mukherjee, Susan L. Bane, Dan L. Sackett -- Live-cell imaging of microtubules and microtubule-associated proteins in arabidopsis thaliana / Jessica Lucas -- Investigating tubulin posttranslational modifications with specific antibodies / Maria M. Magiera, Carsten Janke -- Purification and assembly of bacterial tubulin BtubA/B and constructs bearing eukaryotic tubulin sequences / José M. Andreu, María A. Oliva -- Microtubule-associated proteins and tubulin interaction by isothermal titration calorimetry / P.O. Tsvetkov, P. Barbier, G. Breuzard, V. Peyrot, F. Devred -- Methods for studying microtubule binding site interactions : zampanolide as a covalent binding agent / Jessica J. Field, Enrique Calvo, Peter T. Northcote, John H. Miller, Karl-Heinz Altmann, José Fernando Díaz -- Studying kinetochore-fiber ultrastructure using correlative light-electron microscopy / Daniel G. Booth, Liam P. Cheeseman, Ian A. Prior, Stephen J. Royle -- Fluorescence-based assays for microtubule architecture / Susanne Bechstedt, Gary L. Brouhard -- Structure-function analysis of yeast tubulin / Anna Luchniak, Yusuke Fukuda, Mohan L. Gupta -- Using MTBindingSim as a tool for experimental planning and interpretation / Julia T. Philip, Aranda R. Duan, Emily O. Alberico, Holly V. Goodson -- Imaging individual spindle microtubule dynamics in fission yeast / Judite Costa, Chuanhai Fu, Viktoriya Syrovatkina, Phong T. Tran.
  • sect. I. Isolation and biochemistry of tubulin and characterization of antibodies and isotypes -- Preparation of microtubule protein and purified tupbulin from bovine brain by cycles of assembly and disassembly and phosphocellulose chromatography / Herbert P. Miller, Leslie Wilson -- Isolating tubulin from nonneural sources / Dan L. Sackett, Karl A. Werbovetz, Naomi S. Morrissette -- Characterization of anti-B-tubulin antibodies / Anthony J. Spano, Anthony Frankfurter -- Expression profliing of tubulin isotypes and microtubule-interacting proteins using real-time polymerase chain reaction / Sharon Lobert, Laree Hiser, John J. Correia -- Nondenaturing electrophoresis as a tool to investigate tabulin complexes / Monica Lopez Fanarago... [et al.] -- Mass spectrometry analysis of c-terminal postranslational modifications of tubulins / Virginie Redeker -- Methods in tubulin proteomics / Leah M. Miller... [et al.] -- sect. II. Microtubule structure and dynamics -- Cryo-em studies of microtubule structural intermediates and kinetochore-microtubule interactions / Eva Nogales, Vincent H. Rame, Hong-Wei Wang -- High-resolution imaging of microtubules and cytoskeleton structures by atomic force microscopy / Loic Hamon, Patrick A. Curmi, David Patre -- Using computational modeling to understand microtubule dynamics : a primer for cell biologists / Holly V. Goodson, Ivan V. Gregoretti -- Analysis of dynamic instability of steady-state microtubules in vitro by video-enchanced differential interference contrast microscopy with an appendix by Emin Oroudjev / Mythili Yenjerla, Manu Lopus, Leslie Wilson -- Nanometer-resolution microtubule polymerization assays using optical tweezers and microfabricated barriers/ Blake d. Charlebois, Henry T. Schek III, Alan J. Hunt -- Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy / Christopher Gell... [et al.] -- Studying kinesin motors by optical 3D-nanometry in gliding motility assays / Bert Nitzsche... [et al.] -- sect. III. Drugs -- Analysis of tubulin oligomers by analytical ultricentrifugation / John J. Correia -- Determination of drug binding to mactrotubules in vitro / Jennifer A. Smith, Mary Ann Jordan -- Fluorescence spectroscopic methods to analyze drug-tubulin interactoins / Bhabatarak Bhattacharyya, Sonia Kapoor, Dulal Panda -- Tubulin polymerizatin microassay used to compare ligand eficacy / Ashley Davis... [et al.] -- Fluorescent taxoid probes for microtubule research / Isabel Barasoain, J. Fernando Diaz, Jose M. Andrea -- Binding of vinca domain agents to tubulin : structural and biomedical studies / Anthony Cormier... [et al.] -- Measurement of ligand binding to tubulin by sulfhydryl reactivity / Adrian Begaye, Dan L. Sackett -- sect. IV. Interactions with motors and MAPs -- Probing interactions of tubulin with small molecules, peptides, and protein fragments by solution nuclear magnetic resonance / Marie-Jeanne Clement... [et al.] -- Microtubule and MAPs : thermodynamics of complex formation by AUC, ITC, fluorescence, and NMR / Francois Devred... [et al.] -- Quantitative analysis of MAP-mediated regulation of microtubule dynamic instability in vitro--focus on tau / Erkan Kiris, Donovan Ventimiglia, Stuart C. Feinstein -- Structure and dynamics of the kinesin-Microtubule interaction revealed by fluorescence polarization microscopy / Hernando Sosa, Ana B. Asenjo, Erwin J.G. Peterman -- Multiple collor single molecule TIRF imaging and tracking of MAPs and motors / Jennifer L. Ross, Ram Dixit -- Studying plus-end tracking at single molecule resolution using TIRF microscopy / Ram Dixit, Jennifer L. Ross -- Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor, and +TIP dynamics / Peter Bieling... [et al.] --
  • pt. 1. GENERAL ASPECTS OF MIDKINE -- General Information on Midkine / Takashi Muramatsu -- The Role of Midkine in Neural Development and Patterning / Sheng Yao and Christoph Winkler -- Midkine: A Player in Innate Immunity / Sara L. Nordin and Arne Egesten -- Midkine/P63 Axis in Regulation of Epithelial-Mesenchymal Transition / Yiping Huang and Edward A. Ratovitski -- Immunochemical Methods for the Detection of Midkine in Samples of Human Origin / Stefan Sommerschuh, Michael Kirchner and Nicole Gliese -- Circulating Midkine in Malignant, Inflammatory, and Infectious Diseases: A Systematic Review / Malgorzata Krzystek-Korpacka and Malgorzata Matusiewicz -- pt. 2. MIDKINE AND INFLAMMATION -- Midkine in Inflammation and Inflammatory Diseases / Nuray Yazıhan, Mehtap Kacar Kocak and Ethem Akcıl -- Rheumatoid Arthritis and Midkine / Hisako Muramatsu, Kiyoko Maruyama and Naoki Ishiguro -- pt. 3. MIDKINE AND THE CARDIOVASCULAR SYSTEM -- Midkine and Heart Failure / Mitsuru Horiba and Kenji Kadomatsu -- Vascular Restenosis and Midkine / Kenji Kadomatsu -- The Potential of MK Through the Renin-Angiotensin System / Tomoki Kosugi and Kenji Kadomatsu -- pt. 4. MIDKINE AND THE NERVOUS SYSTEM -- Midkine and Multiple Sclerosis / Hideyuki Takeuchi -- Midkine Gene Transfer in Brain Infarction / Hiroaki Ooboshi, Eiichi Ishikawa, Junichi Takada, Takashi Shichita and Yasuhiro Kumai -- Midkine in Psychiatric and Neurodegenerative Diseases / Eiji Shimizu and Daisuke Matsuzawa -- pt. 5. MIDKINE AND THE UROGENITAL SYSTEM, REPRODUCTION AND AGING -- Role of Midkine in Nephrogenesis and Kidney Disease / Waichi Sato and Kenji Kadomatsu -- Midkine, a Factor Promoting Cytoplasmic Maturation of Oocytes / Masayasu Yamada, Yuuki Isaji and Shuntaro Ikeda -- Endometriosis May Be Promoted by Midkine: Possible Involvement of Ovarian Follicles / Yutaka Osuga, Yasushi Hirota, Kaori Koga, Osamu Yoshino and Tetsuya Hirata -- Stem Cells and Midkine / Hisako Muramatsu, Peng Zou and Takashi Muramatsu -- Midkine in Skeletal Physiology / Astrid Liedert, Thorsten Schinke, Anita Ignatius and Michael Amling -- pt. 6. MIDKINE AND CANCER -- Midkine and Chemoresistance in Cancers / Yoshifumi Takei and Kenji Kadomatsu -- A Gene Medicine with the Midkine-mediated Transcriptional Regulation as New Cancer Therapeutics / Masatoshi Tagawa, Kiyoko Kawamura, Ling Yu, Yuji Tada and Kenzo Hiroshima, et al. -- Midkine as a Tumor-Shared Antigen / Jerome Kerzerho, Florence Anne Castelli and Bernard Maillère -- Midkine in Prostate Cancer / Zongbing You -- Midkine Is a Potential Therapeutic Target of Hepatocellular Carcinoma / Li Cheng Dai, Xing Yao and Jing Zhong -- Midkine in Glioblastoma with the Modulator Role Switching Autophagy to Cell Death or Cell Survival / Mine Ergüven and Ayhan Bilir -- Colon Cancer and Midkine / Özgür Kemik, Ahu Sarbay Kemik and A. Cumhur Dülger -- Osteosarcoma and Midkine / Tadashi Kaname, Kumiko Yanagi and Hiroki Maehara.
  • Regulation of mitochondrial respiration in heart muscle / Ilmo Hassinen -- Regulation of fatty acid oxidation of the heart / Clifford D.L. Folmes and Gary D. Lopaschuk -- Regulation of mitochondrial fuel handling by the peroxisome proliferator-activated receptors / Mary C. Sugden and Mark J. Holness -- Molecular structure of the mitochondrial citrate transport protein / Ronald S. Kaplan and June A. Mayor -- Regulation of pyruvate and amino acid metabolism / Thomas C. Vary, Wiley W. Souba, and Cristopher J. Lynch -- Amino acids and the mitochondria / Nicola King -- Mechanotransduction of shear-stress at the mitochondria / Abu-Bakr Al-Mehdi -- Formation of reactive oxygen species in mitochondria / Julio F. Turrens -- Mitochondrial calcium : role in the normal and ischaemic/reperfused myocardium / Elinor J. Griffiths ... [et al.] -- Mitochondrial ion channels / Brian O'Rourke -- The mitochondrial permeability transition pore : from molecular mechanism to reperfusion injury and cadioprotection / Andrew P. Halestrap, Samantha J. Clarke, and Igor Khalilin -- The apoptotic mitochondrial pathway : modulators, interventions, and clinical implications / M.-Saadeh Suleiman and Stephen W. Schaffer -- The role of mitochondria in necrosis following myocardial ischemia-reperfusion / Elizabeth Murphy and Charles Steenbergen -- Mitochondria and their role in ischemia/reperfusion injury / Sebastian Phillip, James M. Downey, and Michael V. Cohen -- Mitochondrial DNA damage and repair / Inna N. Shokolenko, Susan P. Ledoux, and Glenn L. Wilson.
  • Mitochondria. 2nd ed. 2007
    Also available: Print – 2007
  • Mitochondria, practical protocols 2007.
    Springer Protocols
    Offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike.
  • Mitochondrial cytopathies are mutations in the inherited maternal mitochondrial genome, or the nuclear DNA-mutation. Mitochondrial respiratory chain disorders (RCD) are a group of genetically and clinically heterogeneous diseases, due to the fact that protein components of the respiratory chain are encoded by both mitochondrial and nuclear genomes and are essential in all cells. In addition, the biogenesis, structure and function of mitochondria, including DNA replication, transcription, and translation, all require nuclear encoded genes. Since mitochondria are present in every cell, every tissue, mitochondrial disorders usually affects multiple organs. Mitochondrial Disorders Caused by Nuclear Genes discusses the biochemical, molecular, clinical, and genetic aspects of complex dual genome mitochondrial disorders. Chapters include genes involved in mitochondrial DNA biogenesis and maintenance of mitochondrial DNA integrity, complex subunits and assembly genes, and mitochondrial protein translation related diseases.
  • 1. Clinical Approach to the Diagnosis of Mitochondrial Disease / Dominic Thyagarajan, p. 1-23 -- 2. Mitochondrial DNA Mutations and Their Effects on Complex I Biogenesis: Implications for Metabolic Disease / Matthew McKenzie, p. 25-47 -- 3. Embryonic Stem Cells: A Signalling Perspective / Marios P. Stavridis, p. 49-68 -- 4. From Oocytes and Pluripotent Stem Cells to Fully Differentiated Fates: (Also) a Mitochondrial Odyssey / João Ramalho-Santos, Ana Sofia Rodrigues, p. 69-86 -- 5. From Pluripotency to Differentiation: The Role of mtDNA in Stem Cell Models of Mitochondrial Diseases / João Facucho-Oliveira, Tejal Kulkarni, p. 87-118 -- 6. The Role of Mitochondrial DNA in Tumorigenesis / Ka Yu Yeung, Adam Dickinson, p. 119-155 -- 7. Assisted Reproductive Technologies: The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next / Richard D. W. Kelly, Arsalan Mahmud, p. 157-183.
  • Mitochondria, often referred to as the 'powerhouses' of the cell, generate adenosine triphosphate (ATP) by oxidative phosphorylation or OXPHOS, and maintain cellular homeostasis. In addition to generating ATP, mitochondria are involved in regulation of cell cycle, proliferation, free radical production, innate immune responses and apoptosis. Mitochondrial Function in Lung Health and Disease fills the current gap in the literature and outlines the growing clinical relevance of mitochondrial dysfunction. Currently, there is no overview on the role of mitochondria in pulmonary diseases and this volume focuses on the mitochondrial metabolism, redox signaling, and mechanisms of mitochondrial pathways in lung injury, inflammation, repair and remodeling. Furthermore, in addition to their well-recognized role in cellular energy production and apoptosis, mitochondria appear to play a role in many respiratory diseases and lung cancer. Chapters are written by top notch researchers and clinicians and outline the evidence for mitochondrial biogenesis in inhalational lung injury, COPD and asthma.
  • Introduction to Mitochondrial Oxidative Phosphorylation / Bernhard Kadenbach -- Mitochondrial Dynamics: The Intersection of Form and Function / Andrew Ferree and Orian Shirihai -- Biogenesis of Mitochondrial Proteins / Johannes M. Herrmann, Sebastian Longen, Daniel Weckbecker and Matthieu Depuydt -- Assembly Factors of Human Mitochondrial Respiratory Chain Complexes: Physiology and Pathophysiology / Daniele Ghezzi and Massimo Zeviani -- Supramolecular Organisation of the Mitochondrial Respiratory Chain: A New Challenge for the Mechanism and Control of Oxidative Phosphorylation / Giorgio Lenaz and Maria Luisa Genova -- Molecular Mechanisms of Superoxide Production by the Mitochondrial Respiratory Chain / Stefan Dröse and Ulrich Brandt -- Studies on the Function and Regulation of Mitochondrial Uncoupling Proteins / Richard K. Porter -- Evolution of the Couple Cytochrome c and Cytochrome c Oxidase in Primates / Denis Pierron, Derek E. Wildman, Maik Hüttemann, Thierry Letellier and Lawrence I. Grossman -- Reaction Mechanism of Mammalian Mitochondrial Cytochrome c Oxidase / Shinya Yoshikawa, Kazumasa Muramoto and Kyoko Shinzawa-Itoh -- Phosphorylation of Mammalian Cytochrome c and Cytochrome c Oxidase in the Regulation of Cell Destiny: Respiration, Apoptosis, and Human Disease / Maik Hüttemann, Icksoo Lee, Lawrence I. Grossman, Jeffrey W. Doan and Thomas H. Sanderson -- Individual Biochemical Behaviour Versus Biological Robustness: Spotlight on the Regulation of Cytochrome c Oxidase / Rabia Ramzan, Petra Weber, Bernhard Kadenbach and Sebastian Vogt -- Bigenomic Regulation of Cytochrome c Oxidase in Neurons and the Tight Coupling Between Neuronal Activity and Energy Metabolism / Margaret T. T. Wong-Riley -- Cytochrome c Oxidase and Its Role in Neurodegeneration and Neuroprotection / Susanne Arnold -- The Many Clinical Faces of Cytochrome c Oxidase Deficiency / Salvatore DiMauro, Kurenai Tanji and Eric A. Schon.
  • "This book examines a unique assembly of coordinated mitochondrial functions that are important in regulating cell function and are of relevance in a myriad of pathophysiological situations, such as age-related neurodegenerative disorders, cancer, metabolic syndrome, and cardiovascular disease. The text covers themes essential for the maintenance of mitochondrial activity, including electron transport and energy production, mitochondrial biogenesis and dynamics, mitochondrial signaling, and apoptosis and autophagy. The book features chapters that are reviews of the important topics covered and are written by an impressive cadre of internationally recognized scientist"--Provided by publisher.
  • Also available: Print – 2012
    Introduction: Reminiscing on Models and Modeling / Robert B. Denman -- Probing Astrocyte Function in Fragile X Syndrome / Shelley Jacobs, Connie Cheng and Laurie C. Doering -- Neural Stem Cells / Maija Castrén -- Fragile X Mental Retardation Protein (FMRP) and the Spinal Sensory System / Theodore J. Price and Ohannes K. Melemedjian -- The Role of the Postsynaptic Density in the Pathology of the Fragile X Syndrome / Stefan Kindler and Hans-Jürgen Kreienkamp -- Behavior in a Drosophila Model of Fragile X / Sean M. McBride, Aaron J. Bell and Thomas A. Jongens -- Molecular and Genetic Analysis of the Drosophila Model of Fragile X Syndrome / Charles R. Tessier and Kendal Broadie -- Fragile X Mental Retardation Protein and Stem Cells / Abrar Qurashi, Xuekun Li and Peng Jin -- Manipulating the Fragile X Mental Retardation Proteins in the Frog / Marc-Etienne Huot, Nicolas Bisson, Thomas Moss and Edouard W. Khandjian -- Exploring the Zebra Finch Taeniopygia guttata as a Novel Animal Model for the Speech-Language Deficit of Fragile X Syndrome / Claudia Winograd and Stephanie Ceman -- Neuroendocrine Alterations in the Fragile X Mouse / Abdeslem El Idrissi, Xin Yan, William L'Amoreaux, W. Ted Brown and Carl Dobkin -- Taking STEPs Forward to Understand Fragile X Syndrome / Susan M. Goebel-Goody and Paul J. Lombroso -- Fmr-1 as an Offspring Genetic and a Maternal Environmental Factor in Neurodevelopmental Disease / Bojana Zupan and Miklos Toth -- Mouse Models of the Fragile X Premutation and the Fragile X Associated Tremor/Ataxia Syndrome / Michael R. Hunsaker, Gloria Arque, Robert F. Berman, Rob Willemsen and Renate K. Hukema -- Clinical Aspects of the Fragile X Syndrome / W. Ted Brown -- Fragile X Syndrome: A Psychiatric Perspective / Michael R. Tranfaglia -- Fragile X Syndrome and Targeted Treatment Trials / Randi Hagerman, Julie Lauterborn, Jacky Au and Elizabeth Berry-Kravis -- The Fragile X-Associated Tremor Ataxia Syndrome / Flora Tassone and Randi Hagerman -- Vignettes: Models in Absentia / Robert B. Denman.
  • Growing evidence indicates that cancers are composed of functionally heterogeneous cells that are organized by a cellular hierarchy and are maintained by a stem cell-like population. Given their unique ability to initiate tumor growth, eradication of these cancer stem cells (CSC) is necessary for cure. Thus, the development of CSC targeted therapies is necessary to improve current clinical outcomes. This body of work identifies CD47 as a therapeutic target on CSC and bulk cells in several human malignancies. CD47 is a cell surface protein that functions to inhibit phagocytosis through binding its ligand SIRP[Alpha], expressed on phagocytes. Compared to normal cell counterparts, CD47 was highly expressed on several human tumors including acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), and non-Hodgkin lymphoma (NHL), suggesting that higher CD47 levels on tumors allow evasion of immune phagocytosis. Higher CD47 expression in these tumors correlated with a worse clinical prognosis and was an independent prognostic factor. Furthermore, a monoclonal blocking antibody against CD47 enabled phagocytosis of human AML, ALL, and NHL in vitro and eliminated disease in vivo. When combined with the Fc receptor-activating antibody, rituximab, anti-CD47 antibody therapy achieved high cure rates in pre-clinical models of NHL in contrast to partial responses with either agent alone. Lastly, anti-CD47 antibody treatment specifically targeted tumor but not normal cells, resulting in minimal toxicity in vitro and in vivo. The selective targeting of tumor cells by anti-CD47 antibody was due to the selective expression of the pro-phagocytic signal, calreticulin on tumor but not normal cells. In summary, this body of work identifies CD47 as a therapeutic target on several human cancers and that a monoclonal blocking antibody against CD47 has pre-clinical efficacy in eliminating these tumors through the promotion of phagocytosis.
  • Methods for labeling non-phagocytic cells with MR contrast agents / Joseph A. Frank, Stasia A. Anderson, & Ali S. Arbab -- Cellular imaging of cell transplants / Michel Modo & Jeff W.M. Bulte -- Cellular and molecular imaging of the diabetic pancreas / Zdravka Medarova & Anna Moore -- Functional cellular imaging with manganese / Vincent van Meir & Annemie van der Linden -- Translating promising experimental approaches to clinical trials / Adrian D. Nunn -- An outlook on cellular and molecular MR imaging / Michel Modo & Jeff W.M. Bulte. What is molecular and cellular imaging? / Michel Modo & Jeff W.M. Bulte -- The physicochemical principles of MR contrast agents / Peter Caravan -- Paramagnetic contract agents / Silvio Aime ... [et al.] -- Superparamagnetic contrast agents / Claire Corot ... [et al.] -- Physical mechanism and applications of cest contrast agents / Mike McMahon ... [et al.] -- Paracest contrast agents / A. Dean Sherry & Mark Woods -- Genetic approaches for modulating MRI contrast / Eric Ahrens, Williams F. Goins, Clinton S. Robinson -- Molecular and functional mr imaging of cancer / Michael A. Jacobs ... [et al.] -- Molecular imaging of atherosclerosis with magnetic resonance / Karen C. Briley-Saebo ... [et al.] -- Molecular imaging of apoptosis / Mikko Kettunen & Kevin M. Brindle -- Molecular imaging of reporter genes / Keren Ziv ... [et al.] -- Pharmacological MRI as a molecular imaging technique / Y. Iris Chen & Bruce Jenkins -- Cellular imaging of the liver using contrast agents / N. Cem Balci & Sukru Mehmet Erturk -- Cellular imaging of macrophage activity in infection and inflammation / Martin Rausch ... [et al.] -- Cellular imaging of macrophage activity in stroke / Ralph Weber & Mathias Hoehn -- Magnetic resonance imaging in developmental biology / Cyrus Papan, J. Michael Tyszka, Russell E. Jacobs --
  • Also available: Print – 2010
  • Print Material
  • Print Material
    "As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts. As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced. Protein structures better illustrate structure-function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing open-ended questions highlighting "What We Don't Know," introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text. Thought-provoking end-of-chapter questions have been expanded to all chapters, including questions on developmental biology, tissues and stem cells, the immune system, and pathogens."-- Provided by publisher.
  • Molecular biology of the cell. 4th ed. 2002
    NCBI Bookshelf, Fulltext accessible by its search box
    Also available: Print – 2002
    Also available: Print – 2002
  • Print Material
    Cells and genomes -- Cell chemistry and biosynthesis -- Proteins -- DNA, chromosomes, and genomes -- DNA replication, repair, and recombination -- How cells read the genome: from DNA to protein -- Control of gene expression -- Manipulating proteins, DNA, and RNA -- Visualizing cells -- Membrane structure -- Membrane transport of small molecules and the electrical properties of membranes -- Intracellular compartments and protein sorting -- Intracellular vesicular traffic -- Energy conversion: mitochondria and chloroplasts -- Mechanisms of cell communication -- The cytoskeleton -- The cell cycle -- Apoptosis -- Cell junctions, cell adhesion, and the extracellular matrix -- Cancer -- Sexual reproduction: meiosis, germ cells, and fertilization -- Development of multicellular organisms -- Specialized tissues, stem cells, and tissue renewal -- Pathogens, infection, and innate immunity -- The adaptive immune system. Tables: The genetic code, amino acids.
  • Print Material
    Overview: Molecular Cell Biology presents the key concepts in cell biology and their experimental underpinnings. The authors, all world-class researchers and teachers, incorporate medically relevant examples where appropriate to help illustrate the connections between cell biology and health and human disease. As always, a hallmark of MCB is the use of experiments to engage students in the history of cell biology and the research that has contributed to the field. The book contains predominantly color illustrations, with some black-and-white illustrations.
  • Also available: Print – 2000
  • Also available: Print – 1987
  • Print Material
    Chapter 1. Molecular imaging -- Chapter 2. Basic cell & molecular biology -- Chapter 3. Ovreview of imaging modalities -- Chapter. 4. Molecular imaging strategies -- Chapter 5. Assays -- Chapter 6. Nanoparticle imaging -- Chapter 7. Clinical applications -- Chapter 8. Molecular imaging examples -- Chapter 10. Frequently asked questions -- Chapter 11. Useful tables -- Chapter 12. Probe structures table.
  • Speakers at the Symposium / Hartmann, D. -- Hematopoietic stem cell aging and fate decision / Illing, A., Ju, Z. -- Alterations of systemic environment impair stem cell function during aging / Song, Z., Ju, Z. -- Novel insights into regulation of stem cell aging by aging of the niche and alterations in systemic factors / Geiger, H. -- Aging and hematopoietic malignancies / Gompf, A. -- Intestinal stem cell aging / Sperka, T., Rudolph, K.L. -- Molecular mechanisms of aging in muscle stem cells / Tümpel, S. -- Free radicals and stem cell aging / Guachalla, L.M. -- DNA damage, checkpoint responses, and cell cycle control in aging stem cells / Sperka, T., Nalapareddy, K., Rudolph, K.L.
  • Patients with various cancers are treated with conventional chemotherapeutic drugs and the majority responds well to such therapies. However, there is a subset of patients who does not respond initially and another subset who no longer responds to further treatments. Clearly, in those two subsets of patients, the cancer cells exhibit mechanisms of resistance. One of the main challenges facing us to date is to develop new therapies to treat patients with the resistant tumors. The development of new effective therapies will be dependent on delineating the biochemical, molecular, and genetic mechanisms that regulate tumor cell resistance. Such mechanisms have revealed gene products that directly regulate resistance and are targets for therapy. Of interest, several FDA-approved drugs were able to overcome drug resistance and have been successfully used clinically. They have been used as monotherapy or synergized with other therapies for the treatment of resistant tumors. This volume constitutes a total of twelve selective reviews by pioneer scientists in the field of cancer drug resistance. Specific mechanisms in drug resistance are reviewed and novel approaches are being proposed for therapeutic interventions. This volume is of general interest to scientists, clinicians, health care providers, and students.
  • Introduction -- Morphological aspects of tumor vasculature -- Mechanisms of control of angiogenesis in tumor vasculature -- Anti-Angiogenesis and vascular targeting in tumor vasculature.
  • Part 1. Structure and Function of Mortalin / Birth of Mortalin: Multiple Names, Niches and Functions Connecting Stress, Senescence and Cancer / Renu Wadhwa and Sunil C. Kaul -- Mortalin's Machinery / Custer C. Deocaris, Sunil C. Kaul and Renu Wadhwa -- The Role of Mortalin in Iron Homeostasis / Wen-I Luo and James A. Cowan -- Functional Characteristics of Mortalin / Walter A. Baseler, Tara L. Croston and John M. Hollander -- Part 2. Mortalin in Evolution2 Deocaris / Mortalin and Drosophila DmHsp22: Two Mitochondrial Chaperones Regulating Aging and Carcinogenesis / Marie Le Pécheur, Geneviève Morrow and Robert M. Tanguay -- Mortalin in Invertebrates and The Induction of Apoptosis by Wild-Type p53 Following Defeat of Mortalin-Based Cytoplasmic Sequestration in Cancerous Clam Hemocytes / Charles W. Walker, Ben Low and S. Anne Böttger -- Mortalin and Stem Cells: A Study from Planarians / Renata Batistoni -- Part 3. Mortalin in Health and Disease / Mortalin in Cell Protection from Immune Attack / Moran Saar, Oren Moskovich and Zvi Fishelson -- Mortalin in Neurological Diseases / Jinghua Jin, Jing Zhang, Travis J. Cook and Jake G. Hoekstra -- Loss of Mortalin Function in Parkinson's Disease-Supporting the Mitochondrial Pathway of Neurodegeneration / Lena F. Burbulla and Rejko Krüger -- Hsp75/mortalin and Protection from Ischemic Brain Injury / Robin E. White, Yi-Bing Ouyang and Rona G. Giffard -- Catecholamine Regulated Protein (CRP40), A Splice Variant of Mortalin-2: Functional Role in CNS Disorders / Joseph P. Gabriele, Sarah E. Groleau, Ritesh P. Daya, Zdenek B. Pristupa and Ram K. Mishra -- Chaperonopathies: Diseases in Which Mortalin and Other Hsp-Chaperones Play a Role in Etiology and Pathogenesis / Alberto J. L. Macario, Francesco Cappello and Everly Conway de Macario -- Part 4. Mortalin and Cancer / Many Faces of Mortalin and Tid1 / Ohad Iosefson and Abdussalam Azem -- Mortalin: A Positive Regulator of Centrosome Duplication and Amplification / Masayuki Kanai and Kenji Fukasawa -- Mortalin Expression in Normal and Neoplastic Tissues / Angheliki Nomikos, Sinclair R. Dundas and Graeme I. Murray -- Mortalin-p53 Interaction as a Target for Liver Cancer Therapy / Wen-Jing Lu, Nikki P. Lee, Renu Wadhwa and John M. Luk -- Mortalin Targeting Gadgets for Cancer Therapy / Chae-Ok Yun and Renu Wadhwa -- Part 5. Mortalin Based Technologies / Cell Internalizing Anti-Mortalin Antibody for Generation of Illuminating MSCs for Long-Term In vitro and In vivo Tracking / Toshimasa Uemura, Masanori Nishi, Sunil C. Kaul and Renu Wadhwa -- Mortalin Staining Pattern as a Reporter for Cell Based Anti-Cancer Drug Screening / Ran Gao, Zeenia Kaul, Tomoko Yaguchi and Renu Wadhwa -- Cell Internalizing Anti-mortalin Antibody as a Nanocarrier / Zeenia Kaul, Tomoko Yaguchi, Renu Wadhwa and Sunil C. Kaul.
  • Also available: Print – 2012
    Post-transcriptional Control of Gene Expression During Mouse Oogenesis / Hugh J. Clarke -- Polarity and Asymmetry During Mouse Oogenesis and Oocyte Maturation / Malgorzata Kloc, Rafik M. Ghobrial, Ewa Borsuk and Jacek Z. Kubiak -- Chromatin Structure and ATRX Function in Mouse Oocytes / Rabindranath De La Fuente, Claudia Baumann and Maria M. Viveiros -- Cyclin B in Mouse Oocytes and Embryos: Importance for Human Reproduction and Aneuploidy / Zbigniew Polański, Hayden Homer and Jacek Z. Kubiak -- Src Protein Kinases in Mouse and Rat Oocytes and Embryos / Mattan Levi, Lihi Ninio-Mani and Ruth Shalgi -- Gradual Meiosis-To-Mitosis Transition in the Early Mouse Embryo / Aurélien Courtois and Takashi Hiiragi -- Maternal Control of Mouse Preimplantation Development / Wenjing Zheng and Kui Liu -- Preimplantation Mouse Embryo: Developmental Fate and Potency of Blastomeres / Aneta Suwińska -- Creation of Trophectoderm, the First Epithelium, in Mouse Preimplantation Development / Yusuke Marikawa and Vernadeth B. Alarcon -- Cell Lineage Allocation Within the Inner Cell Mass of the Mouse Blastocyst / Panagiotis Xenopoulos, Minjung Kang and Anna-Katerina Hadjantonakis -- Formation of Distinct Cell Types in the Mouse Blastocyst / Samantha A. Morris and Magdalena Zernicka-Goetz -- Cell Movements in the Egg Cylinder Stage Mouse Embryo / Bradley Joyce and Shankar Srinivas -- Balancing the Dose in the Mouse / Mary E. Donohoe -- Mouse Oviduct Development / C. Allison Stewart and Richard R. Behringer -- Cell Lineages, Growth and Repair of the Mouse Heart / Fabienne Lescroart and Sigolène M. Meilhac -- Cellular Reprogramming During Mouse Development / Zoë D. Burke, Gabriela Miron-Buchacra and David Tosh -- Differentiation of Definitive Endoderm from Mouse Embryonic Stem Cells / Peter T. W. Kim and Christopher J. Ong -- Mouse and Human Pluripotent Stem Cells and the Means of Their Myogenic Differentiation / I. Grabowska, K. Archacka, A. M. Czerwinska, M. Krupa and M. A. Ciemerych -- Stem Cells and Corneal Epithelial Maintenance: Insights from the Mouse and Other Animal Models / Richard L. Mort, Panagiotis Douvaras, Steven D. Morley, Natalie Dorà and Robert E. Hill, et al. -- Mouse-Induced Pluripotent Stem Cells / Hui Zhang and Ying Jin -- Aging in the Mouse and Perspectives of Rejuvenation Through Induced Pluripotent Stem Cells (iPSCs) / Ken-ichi Isobe, Zhao Cheng, Sachiko Ito and Naomi Nishio.
  • Muscarinic receptor pharmacology and signaling -- Overview of muscarinic receptor subtypes -- Structure-function studies of muscarinic acetylcholine receptors -- Polymorphisms in human muscarinic receptor subtype genes -- Muscarinic receptor trafficking -- Physiological role of G-protein coupled receptor phosphorylation -- Novel muscarinic receptor mutant mouse models -- Muscarinic receptors in the CNS -- Muscarinic receptor pharmacology and circuitry for the modulation of cognition -- Muscarinic agonists and antagonists in schizophrenia -- Muscarinic pain pharmacology: realizing the promise of novel anagesics by overcoming old challenges -- Muscarinic modulation of striatal function and circuitry -- Muscarinic receptors in brain stem and mesopontine cholinergic arousal functions -- Muscarinic receptors in autonomic effector organs -- Muscarinic receptor agonists and antagonists: effects on ocular function -- Muscarinic receptor agonists and antagonists: effects on cardiovascular function -- Muscarinic receptor antagonists: effects on pulmonary function -- Muscarinic agonists and antagonists: effects on gastrointestinal function -- Muscarinic agonists and antagonists: effets on the urinary bladder -- Muscarinic receptors and mediation of hormonal effects of acetylcholine -- Muscarinic receptor agonists and antagonists: effects on inflammation and immunity -- Muscarinic receptor agonists and antagonists: effects on keratinocyte functions -- Muscarinic receptor agonists and antagonists: effects on cancer -- Activation of muscarinic receptors by non-neuronal acetylcholine.
  • An introduction to muscle development in drosophila / Helen Sink -- The muscle pattern of drosophila / Volker Hartenstein -- Mesoderm formation in the drosophila embryo / Noriko Wakabayashi-Ito and Y. Tony Ip -- Development of the cardiac musculature / Rolf Bodmer -- Development of the somatic gonad and fat bodies / Mark Van Doren -- Development of the larval visceral musculature / Hsiu-Hsiang Lee, Stephane Zaffran and Manfred Frasch -- Development of the larval somatic musculature / Ana Carmena and Mary Baylies -- Muscle morphogenesis: the process of embryonic myoblast fusion / Susan M. Abmayr and Kiranmai S. Kocherlakota -- Muscle attachment sites-where migrating muscles meet their match / Talila Volk -- Neuromuscular development: connectivity and plasticity / Louise Nicholson and Haig Keshishian -- Metamorphosis and the formation of the adult musculature / Devkanya Dutta and K. VijayRaghavan -- Molecular basis of muscle structure / Jim O. Vigoreaux -- Whole genome approaches to studying drosophila muscle development / Eileen E.M. Furlong -- Comparison of muscle development in drosophila and vertebrates / Michael V. Taylor.
  • This book provides the readers with an overview of research on p53, which has been shown to play a role in numerous crucial biological pathways in normal and cancer cells. Leading scientist in the field, who have all made direct contributions to the understanding of the molecular events underpinning p53 function, have been invited to contribute the various chapters, which discuss the current knowledge of the signaling cascades that are activated by mutations in p53 and overexpression of MDM2, frequently found in human cancer, and are major causes of oncogenesis. This book features chapters on the molecular basis of oncogenesis induced by gain of function mutation of p53, signaling pathways induced by MDM2 overexpression, control of mutant or wild-type p53 function by MDM2 and MDMX, p53 mutation in hereditary cancer, and structural aspects that activate mutant p53 which can be targeted by drug therapy. This book should be useful for scientists at all levels.
  • Myc gene : methods and protocols 2013
    Springer Protocols
  • Porosomes--the universal secretory portals in cells: a brief essay / Bhanu P. Jena -- The hair cell porosome: molecular and synaptic implications / Dennis G. Drescher -- The neuronal porosome complex in mammalian brain: a study using electron microscopy / Mzia G. Zhvania, Nadezhda J. Japaridze, Mariam G. Qsovreli, Vera G. Okuneva, Arkadi G. Surmava, and Tamar G. Lordkipanidze -- Granule size distribution suggests mechanism: the case for granule growth and elimination as a fusion nano-machine / Ilan Hammel and Isaac Meilijson -- Probing protein assembly, biomineralization, and biomolecular interactions by atomic force microscopy / Kang Rae Cho and James J. De Yoreo -- High-resolution imaging of amylin aggregation and internalization in pancreatic cells: implications in health and disease / Saurabh Trikha, Sanghamitra Singh, and Aleksandar M. Jeremic -- Repair of Nanodefects in a two-dimensional crystal anticoagulant shield in the antiphospholipid syndrome: novel molecular strategies assessed by atomic force microscopy / Douglas J. Taatjes, Anthony S. Quinn, Xiao-Xuan Wu, Han-Mou Tsai, and Jacob H. Rand -- A novel approach to study molecular evolution: detection of ancestral conformation hidden in present-day proteins using antibody as nanostructure probes / Jian-Ping Jin -- mRNA nanomachines and stress reprogramming following brain ischemia / Donald J. DeGracia, Jill T. Jamison, Manupreet Chawla, Monique K. Lewis, Michelle Smith, and Jeffery J. Szymanski -- Physical properties and biomedical applications of superparamagnetic iron oxide nanoparticles / Gavin Lawes, Ratna Naik, and Prem Vaishnava -- Atomic force microscopy imaging of DNA delivery nanosystems / Yi Zou, David Oupicky, and Guangzhao Mao -- Impedance spectroscopy for characterization of biological function / Brian C. Riggs, Janet L. Paluh, G.E. Plopper, and Douglas B. Chrisey.
  • Also available: Print – 2014
    Presence in, and release of, nanomaterials from consumer products / Yu Yang and Paul Westerhoff -- Nanoparticle aggregation: principles and modeling / Wen Zhang -- Influences of nanomaterials on the barrier function of epithelial cells / Shariq Ali and Erik Rytting -- Engineered nanoparticles induced brush border disruption in a human model of the intestinal epithelium / James J. Faust, Benjamin M. Masserano, Adam H. Mielke, Anup Abraham, and David G. Capco -- Nanoparticles: cellular uptake and cytotoxicity / Isaac M. Adjei, Blanka Sharma and Vinod Labhasetwar -- Atomic force microscopy study of the interaction of DNA and nanoparticles / Kungang LI, Songyan Du, Steven Van Ginkel, and Yongsheng Chen -- Intracellular signal modulation by nanomaterials / Salik Hussain, Stavros Garantziotis, Fernando Rodrigues-Lima, Jean-Marie Dupret, Armelle Baeza-Squiban, Sonja Boland -- Nanomaterials: impacts on cells and cell organelles / Željka Krpetić, Sergio Anguissola, David Garry, Philip Kelly and Kenneth A. Dawson -- Design, synthesis, and functionalization of nanoparticles for therapeutic drug delivery / Taraka Sai Pavan Grandhi and Kaushal Rege -- Preparation of nanoscale pulmonary drug delivery formulations by spray drying / Adam Bohr, Christian A. Ruge, and Moritz Beck-Broichsitter -- Nanomedicine: the promise and challenges in cancer chemotherapy / Youssef W. Naguib and Zhengrong Cui -- Transgenerational effects of NMs / Anna Poma, Sabrina Colafarina, Gabriella Fontecchio, and Giuseppe Chichiricco -- Stem cells and nanomaterials / Maria-Claude Hofmann.
  • Chapter 1, Developmental stages and pathways of NK cell maturation / Bartosz Grzywacz, Jeffery S. Miller, Michael R. Verneris -- Chapter 2. Developmental origins of thymus-derived natural killer cells / James R. Carlyle, Juan C. Zúñiga-Pflücker -- Chapter 3. Interferon-producing killer dendritic cells (IKDC) / Franck Housseau -- Chapter 4. Natural killer cells in cancer / Petter Höglund, Hans-Gustaf Ljunggren -- Chapter 5. NK cell immune recognition: NKG2D ligands and stressed cells / Carlos López-Larrea, Alejandro López-Soto, Segundo González -- Chapter 6. NK cell KIR heterogeneity and evolution / Makoto Yawata, Nobuyo Yawata -- Chapter 7. Signalling events in natural killer cells / P.K. Epling-Burnette, Sheng Wei, Julie Y. Djeu -- Chapter 8. Human natural killer cell development / Edward L. Briercheck, Aharon G. Freud, Michael A. Caligiuri -- Chapter 9. Isolation, culture and propagation of natural killer cells / Michael Magee, Lazar N. Vujanovic, Lisa H. Butterfield, Nikola L. Vujanovic -- Chapter 10. The localization and migration of natural killer cells in health and disease / Vasileios Bekiaris, Peter J.L. Lane -- Chapter 11. Receptors on NK cells / Noam Stern-Ginossar, Ofer Mandelboim -- Chapter 12. Development and testing of NK cell lines / Hans Klingemann -- Chapter 13. NK cell-derived cytokines and delivery: NK cell synapses / Claudia Semino, Anna Rubartelli -- Chapter 14. NK cells as recipients of cytokine signals / Tara J. Loux, Michael T. Lotze, Herbert J. Zeh III -- Chapter 15. NK cells and chemokines / Angela Gismondi, Giovanni Bernardini, Angela Santoni -- Chapter 16. Natural killer cytolytic activity / Lesley R. de Armas, Eckhard R. Podack -- Chapter 17. Illuminating intranodal natural killer cell behaviour using two-photon microscopy / Kym R. Garrod, Michael D. Cahalan -- Chapter 18. Dendritic cells and NK cells / Amy K. Wesa, Robbie B. Mailliard -- Chapter 19. NK and NKT cells: The innate-adaptive interface including humoral responses / Jeff Subleski, Jonathan M. Weiss, Robert H. Wiltrout, John R. Ortaldo -- Chapter 20. NK cell and endothelial cell interactions / Angela Gismondi, Claudia Carlino, Helena Stabile, Angela Santoni -- Chapter 21. Natural killer cell/epithelial interactions / Taisuke Ito, Masao Takei, Ralf Paus -- Chapter 22. NK cell-T cell interactions / Benedict J. Chambers -- Chapter 23. NK cells and NKT cells in cutaneous disorders / Anna Balato, Anthony A. Gaspari -- Chapter 24. Natural killer cells in the respiratory tract / Borna Mehrad -- Chapter 25. Intestinal natural killer cells / Martin R. Goodier -- Chapter 26. Natural killer cells in the liver / Shiguang Qian, Jia Fan, Shuang-Jian Qiu, Yong-sheng Xiao, Lina Lu -- Chapter 27. Natural killer cells in the spleen and lymph node / Alex Karlsson-Parra -- Chapter 28. Nature killer cells in the central nervous system / Fu-Dong Shi, Richard M. Ransohoff -- Chapter 29. NK cells in the eye / Jerry Y. Niederkorn -- Chapter 30. NK cells and reproduction / Ashley Moffett, Victoria H. Male -- Chapter 31. Identification of natural killer cells in tissues and their isolation / Paolo Carrega, Erika Montalto, Guido Ferlazzo -- Chapter 32. Natural killer cell immune regulation: Coordination of immune function in tissues / Lorenzo Moretta, Alessandro Moretta -- Chapter 33. Natural killer cells and transplantation / Benjamin M. Matta, Angus W. Thomson -- Chapter 34. Natural killer cells and autoimmunity / Christian Münz -- Chapter 35. Interaction of NK cells with bacteria / Kenji Chamoto, Daiko Wakita, Shinichi Koizumi, Kazutaka Masuko, Takayuki Ikeda, Rieko Mitamura, Takashi Nishimura -- Chapter 36. Natural killer cells and human immunodeficiency virus / Domenico Mavilio, Anne Hosmalin, Daniel Scott-Algara -- Chapter 37. Cytomegalovirus infection and NK cells / Jerome D. Coudert, Christopher E. Andoniou, Mariapia A. Degli-Esposti -- Chapter 38. Natural killer cells in allergy / Syh-Jae Lin, Yen-Chang Lee, Dah-Chin Yan, Ming-Ling Kuo -- Chapter 39. Natural killer cells in wound healing / Jussi Liippo, Mervi Toriseva, Veli-Matti Kähäri -- Chapter 40. Natural killer cells in atherosclerosis / Godfrey S. Getz, Catherine A. Reardon -- Chapter 41. Natural killer cells in leukaemia / Jérôme Rey, Cyril Fauriat, Alessandro Moretta, Régis Costello, Daniel Olive -- Chapter 42. Natural killer cells and allogeneic haematopoietic cell transplantation / Andrea Velardi -- Chapter 43. Clinical trials of NK cells for cancer / Sarah Cooley, Jeffrey S. Miller -- Chapter 44. Natural killer cells and hepatitis C virus infection / Michael A. Nalesnik, Tatsuya Kanto -- Chapter 45. Natural killer cells and the biology of parasitism / Marianne A. Bryan, Karen A. Norris -- Chapter 46. Lymphoproliferative disorders of natural killer cells / William G. Morice -- Chapter 47. Natural killer cell induction of tolerance / Lina Lu, Alexandra Y. Zhang, William L. Camp, Shiguang Qian -- Chapter 48. Genetic engineering of natural killer cells / Stephen R. Goding, Per H. Basse -- Chapter 49. NK cell-mediated target cell death / Beverly Z. Packard, Akira Komoriya, David S. Ucker -- Chapter 50. NK-cell tracking using non-invasive imaging modalities / Priyanka Jha, Verena Reinhart, Christiane Knopp, Siyuan Liu, Winfried S. Wels, Michael F. Wendland, Heike Daldrup-Link.
  • Introduction : mechanisms of NKT-cell-mediated adjuvant activity and function of iPS-derived NKT cells / Masaru Taniguchi ... [et al.] -- Structure and recognition of antigens for invariant NKT cells / Bo Pei and Mitchell Kronenberg -- Invariant NKT cell-based vaccine strategies / John-Paul Jukes ... [et al.] -- Immune regulation of tumor immunity by NKT cells / Jessica J. O'Konek, Jay A. Berzofsky, and Masaki Terabe -- The regulation of CD1d+ and CD1d- tumors by NKT cells : the roles of NKT cells in regulating CD1d+ and CD1d- tumor immunity / Jianyun Liu, Gourapura J. Renukaradhya, and Randy R. Brutkiewicz -- DC-based immunotherapy targeting NKT cells / Shin-ichiro Fujii and Kanako Shimizu -- Therapeutic approaches utilising NKT cells / Stephen R. Mattarollo and Mark J. Smyth -- NKT cells or cancer patients and how models can inform therapeutic plans / Mark A. Exley, Lydia Lynch, and Michael Nowak -- Understanding the role of natural killer T cells in hematologic malignancies : progress and challenges / Natalia Neparidze and Madhav V. Dhodapkar -- Clinical trials with alpha-galactosylceramide (KRN7000) in advanced cancer / Famke L. Schneiders ... [et al.] -- Clinical trials of invariant natural killer T cell-based immunotherapy for cancer / Shinichiro Motohashi, Yoshitaka Okamoto, and Toshinori Nakayama.
  • Necrosis : methods and protocols 2013
    Springer Protocols
    Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. -- Publisher website.
  • This book summarizes discoveries of historical significance and provides in-depth, current analyses of the evolution of neural crest cells, their contribution to embryo development, and their roles in disease. In addition, prospects for tissue engineering, repair and regeneration are covered, offering a timely synthesis of the current knowledge in neural crest cell research.
  • Defining neural stem cells and their role in normal development of the nervous system / Sally Temple -- Embryonic stem cells and neurogenesis / Robin L. Wesselschmidt -- The cellular and molecular properties of multipotent neural stem cells / Scott R. Hutton and Larysa H. Pevny -- Multipotent stem cells in the embryonic nervous system / Chian-Yu Peng ... [et al.] -- Neural crest formation and diversification / Marcos S. Simões-Costa ... [et al.] -- Glial nature of adult neural stem cells : neurogenic competence in adult astrocytes / William W. Hwang, Arturo Alverez-Buylla and Daniel A. Lim -- Neural stem cell death regulation in nervous system development and disease / K.C. Walls and Kevin A. Roth -- Stem cells of adult olfactory epithelium / James E. Schwob, Woochan Jang and Eric H. Holbrook -- Retinal stem cells / Carolina B. Del Debbio ... [et al.] -- Transdifferentiation in the nervous system / Ying Liu and Mahendra S. Rao -- Neural and dopaminergic differentiation of human pluripotent stem cells / Olga Momcilovic and Xianmin Zeng -- Mobilization of neural stem cells in the adult central nervous system / Harish Babu, Theo D. Palmer and Fred H. Gage -- Stem cell pathways in brain tumors / Justin D. Lathia ... [et al.].
  • Definitions and criteria for stem cells / Leslie P. Weiner -- Neural stem cells and the future treatment of neurological diseases : raising the standard / Jaime Imitola and Samia J. Khoury -- Neural differentiation of human embryonic stem cells / Mirella Dottori and Martin F. Pera -- Generating neurons from stem cells / Andreas Androutsellis-Theotokis ... [et al.] -- Isolation of neural stem and precursor cells from rodent tissue / Yuanyuan Wu ... [et al.] -- Adult rodent spinal cord-derived neural stem cells : isolation and characterization / Lamya S. Shihabuddin -- Adult neural stem cells / Rossella Galli, Angela Gritti, and Angelo L. Vescovi -- Olfactory ensheathing cells : isolation and culture from the neonatal olfactory bulb / Susan C. Barnett and A. Jane Roskams -- Culturing olfactory ensheathing cells from the mouse olfactory epithelium / Miranda Richter, Kathryn Westendorf, and A. Jane Roskams -- Production and characterization of immortal human neural stem cell line with multipotent differentiation property / Seung U. Kim ... [et al.] -- Preparation of neural progenitors from bone marrow and umbilical cord blood / Shijie Song and Juan Sanchez-Ramos -- Production of neurospheres from CNS tissue / Gregory P. Marshall II ... [et al.] -- Nuclear transfer to study the nuclear reprogramming of human stem cells / Shigeo Saito ... [ et al.] -- Clonal analyses and cryopreservation of neural stem cell cultures / Angela Gritti, Rossella Galli, and Angelo L. Vescovi -- Telomere neurobiology / Mark P. Mattson, Peisu Zhang, and Aiwu Cheng -- Chromosomal mosaicism in neural stem cells / Suzanne E. Peterson ... [et al.] -- In vitro assays for neural stem cell differentiation : induction of dopaminergic phenotype / Marcel M. Daadi -- In vitro migration assays of neural stem cells / Pascale Durbec .. [et al.] -- Measuring apoptosis in neural stem cells / Brett T. Lund and Eve E. Kelland -- Markers of adult neural stem cells / Eric Wexler -- Differential gene expression in ES-derived neural stem cells by using RT-PCR / Nicole Slawny, Crystal Pacut, and Theresa E. Gratsch -- Microfluidic devices for high-throughput gene expression profiling of single hESC-derived neural stem cells / Yan Chen and Jiang F. Zhong -- Electroporation strategies for genetic manipulation and cell labeling / Terence J. Van Raay, Rhonda T. Lassiter, and Michael R. Stark -- Novel and immortalization-based protocols for the generation of neural CNS stem cell lines for gene therapy approaches / Luciano Conti and Elena Cattaneo -- Identification of newborn cells by BrdU labeling and immunocytochemistry in vivo / Sanjay S. Magavi and Jeffrey D. Macklis -- Immunocytochemical analysis of neuronal differentiation / Sanjay S. Magavi and Jeffrey D. Macklis -- Neuroanatomical tracing of neuronal projections with Fluoro-Gold / Lisa A. Catapano, Sanjay S. Magavi, and Jeffrey D. Macklis -- Labeling stem cells in vitro for identification of their differentiated phenotypes after grafting into the CNS / Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore -- Optimizing stem cell grafting into the CNS / Scott R. Whittemore ... [et al.] -- Routes of stem cell administration in the adult rodent / Alison E. Willing ... [et al.]
  • "The importance of the regulation of salt is of clinical relevance given its relation to the "epidemic" of high blood pressure and that virtually all foods throughout the EU and USA will be mandated to reduce their salt content. This book covers virtually all aspects of the innate drives of thirst and salt, including transduction, sensory mechanism, afferent systems, organization, CNS integrative circuitry, and efferent pathways and effectors systems. It also covers the addition aspects of salt appetites and the orexigenic aspects, as well as other emerging topics in this field"--Provided by publisher.
  • Also available: Print – 2013
    Altered histone deacetylase activity and iNOS expression in cells isolated from induced sputum of COPD patients treated with tiotropium -- Involvement of NF-[kappa]B and muscle specific E3 ubiquitin ligase MuRF1 in cigarette smoke-induced catabolism in C2 myotubes -- Siglec-8 in induced sputum of COPD patients -- Essential amino acid leucine and proteasome inhibitor MG132 attenuate cigarette smoke induced catabolism in C2 myotubes -- Smoking denial in cardiovascular disease studies -- Differential effects of kainic acid lesions in medullary raphe on cough and sneeze in anesthetized rabbits -- Detection of Chlamydophila pneumoniae antigens in patients with chronic cough -- Antioxidant activity of herbal polysaccharides and cough reflex -- Cytoglobin and neuroglobin in the human brainstem and carotid body -- Flow cytometry in the diagnosis of influenza -- Accuracy of rapid influenza detection test in diagnosis of influenza A and B viruses in children less than 59 months old -- Virological monitoring of influenza activity and influenza-like illness in the epidemic season 2011-2012 in Poland -- Incidence of Chlamydophila pneumoniae infection in children during 2007-2010 -- Clinical features and outcomes of influenza A and B infections in children -- Respiratory complications in children hospitalized with Varicella -- Hyponatremia in children hospitalized due to pneumonia -- In vitro sensitivity of Acinetobacter baumannii and Pseudomonas aeruginosa to carbapenems among intensive care unit patients -- Prevalence of acute respiratory tract diseases among soldiers deployed for military operations in Iraq and Afghanistan -- In vitro susceptibility of Staphylococci and Enterococci to vancomycin and teicoplanin -- Sinusitis in people living in the Medieval Ages -- Immunization coverage against capsular bacteria in splenectomized patients -- Umbilical cord blood gas content, postnatal state of neonates, and lactation after caesarean and natural childbirth -- Respiratory function in pregnant women -- Lung function in patients with gastro-esophageal reflux disease and respiratory symptoms -- Efficacy of noninvasive mechanical ventilation in obese patients with chronic respiratory failure -- Near-infrared hemoencephalography for monitoring blood oxygenation in prefrontal cortical areas in diagnosis and therapy of developmental dyslexia -- Occlusal stabilization splint therapy in orofacial pain and tension-type headache -- Dynamics of upper airways during the Müller maneuver in healthy subjects: a cine MRI study -- EMG analysis of human inspiratory muscle resistance to fatigue during exercise -- Assessment of temporomandibular joint disease -- Spirometry Day: a means to enhance social knowledge on respiratory diseases -- Lung function in divers -- Influence of traffic-related air pollutants on lung function -- Fractional exhaled nitric oxide in clinical trials: an overview -- Real-time breath analysis in type 2 diabetes patients during cognitive effort -- Exhaled nitric oxide in specific inhalation challenge -- Integration of simulated multipotential signals: the role of integration window width and of the number of spikes -- Functional and topographic concordance of right atrial neural structures inducing sinus tachycardia -- Occurrence of disseminated intravascular coagulation in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced pneumonia in the rat -- Implementation of non-invasive methods in the diagnosis of diisocyanate-induced asthma -- Evaluation of a 4-steps-1-day whole body challenge protocol for the diagnosis of occupational asthma due to diisocyanates -- Occupational allergic respiratory diseases in garbage workers: relevance of molds and actinomycetes -- Th1, Th2, Th17, and regulatory cytokines in children with different clinical forms of allergy -- Soluble receptor for urokinase plasminogen activator in community-acquired pneumonia in children -- Hypersensitivity pneumonitis due to metalworking fluids: how to find the antigens -- Angiogenic axis angiopoietin-1 and angiopoietin-2/tie-2 in non-small cell lung cancer: a bronchoalveolar lavage and serum study -- Cancer incidence in pulmonary vasculitis -- Influence of delays in diagnosis and treatment on survival in small cell lung cancer -- Familial idiopathic pulmonary fibrosis -- High resolution computed tomography in 2-year follow-up of stage I sarcoidosis -- Altruistic aptitude: age-dependent influence of temperament and emotional intelligence -- Effect of socio-economic status on quality of life in people affected with respiratory allergy -- Exposure to paracetamol and antibiotics in early life and elevated risk of asthma in childhood -- Health behaviors and quality of life among patients with chronic respiratory disease -- Attitudes toward euthanasia among Polish physicians, nurses and people who have no professional experience with the terminally ill -- Death as a result of violent asphyxia in autopsy reports -- Respiratory and systemic infections in children with severe aplastic anemia on immunosupressive therapy.
  • Nature of neurons and their specialized membranes -- DHA contributes both benefits and risks to mammals -- Use it or lose it concept of brain health is linked to DHA -- Evolution and DHA : redefining the DHA principle -- DHA is king omega for maximizing mental speed -- DHA improves energy efficiency in neurons -- Wild conformational dynamics of DHA might save energy by sealing stretch-induced defects as fast as they occur in membranes -- Lipid whisker model and water-wire theory of energy uncoupling in neurons -- Neurons boost their energy state by using powerful antioxidant systems to protect their membranes against damage -- Oxygen-dependent damage (lipid peroxidation) of DHA membranes of neurons is inevitable and requires novel mechanisms for long-term protection -- Revised mitochondrial theory of aging and brain span -- Development of dual energy pacemaker theory of aging : role of the membrane -- Membranes and cancer -- Parkinson's disease -- Prion diseases -- Brain trauma-induced dementia -- Alzheimer's disease.
  • Neuronal death and neuroprotection : a review / Mariaelena Repici, Jean Mariani, and Tiziana Borsello -- The use of propidium iodide to assess excitotoxic neuronal death in primary mixed cortical cultures / Anthony C. Lau, Hong Cui, and Michael Tymianski -- A modified silver technique (de Olmos Stain) for assessment of neuronal and axonal degeneration / Tatyana I. Tenkova and Mark P. Goldberg -- Model of acute injury to study neuroprotection / Michal Schwartz and Jonathan Kipnis -- Organotypic entorhino-hippocampal slice cultures : a tool to study the molecular and cellular regulation of axonal regeneration and collateral sprouting in vitro / Domenico Del Turco and Thomas Deller -- Role of Nrf2-dependent ARE-driven antioxidant pathway in neuroprotection / Jiang Li ... [et al.] -- Biochemical methods to assess the coupling of brain energy metabolism in control and disease states / Jennifer Zechel, W. David Lust, and Michele Puchowicz -- A method for isolating prosurvival targets of NF-[kappa]B/rel transcription factors / Christian Kuntzen ... [et al.] -- Functional cloning of genes regulating apoptosis in neuronal cells / Roberta Visconti and Luciano D'Adamio -- Characterization of mRNA expression in single neurons / David M. Lin ... [et al.] -- Brain on a chip : a method to detect novel neuroprotective candidate targets / Yang Tang and Myriam Bernaudin -- Intracerebral infusion of neurotrophic factors / Theo Hagg -- Synthesis of cell-penetrating peptides and their application in neurobiology / Gunnar P.H. Dietz and Mathias Bähr -- The stem cells as a potential treatment for neurodegeneration / Ferrari Daniela, Angelo Luigi Vescovi, and Daniele Bottai -- In vivo tomographic imaging studies of neurodegeneration and neuroprotection : a review / Rikki N. Waterhouse and Jun Zhao.
  • Neutrophil methods and protocols 2007
    Springer Protocols
    The role of neutrophils in the immune system: an overview / H.L. Malech -- Subcellular fractionation of human neutrophils and analysis of subcellular markers / L. Udby and N. Borregaard -- Rac and Rap GTPase activation assays / U.G. Knaus, A. Bamberg and G.M. Bokoch -- Measurement of phospholipid metabolism in intact neutrophils / S. Sergeant and L.C. McPhail -- Analysis of protein phosphorylation in human neutrophils / J. El-Benna and P.M.-C. Dang -- Mitogen-activated protein kinase assays / M. Torres -- Transduction of proteins into intact neutrophils / T. Zhao and G.M. Bokoch -- Optical methods for the measurement and manipulation of cytosolic free calcium in neutrophils / E.J. Hillson, S. Dewitt and M.B. Hallett -- Analysis of electrophysiological properties and responses of neutrophils / D. Morgan and T.E. DeCoursey -- Analysis of neutrophil apoptosis / E.L. Taylor ... [et al.] -- Optical and fluorescence detection of neutrophil integrin activation / U.Y. Schaff ... [et al.] -- Analysis of neutrophil polarization and chemotaxis / M.A. Lokuta, P.A. Nuzzi and A. Huttenlocher -- Quantifying and localizing actin-free barbed ends in neutrophils / M. Glogauer -- Measurement of neutrophil adhesion under conditions mimicking blood flow / M.A. Jutila ... [et al.] -- Model systems to investigate neutrophil adhesion and chemotaxis / N.A. Louis, E. Campbell and S.P. Colgan -- Immunofluorescence and confocal microscopy of neutrophils / L.-A.H. Allen -- Assessment of phagosome formation and maturation by fluorescence microscopy / B.E. Steinberg and S. Grinstein -- Analysis of neutrophil membrane traffic during phagocytosis / P. Loennbro, P. Nordenfelt and H. Tapper -- Analysis of neutrophil bactericidal activity / J.N. Green, C.C. Winterbourn and M.B. Hampton -- A skin chamber technique as a human model for studies of aseptic inflammatory reactions / P. Follin and C. Dahlgren -- Measurement of respiratory burst products generated by professional phagocytes / C. Dahlgren, A. Karlsson and J. Bylund -- The K-562 cell model for analysis of neutrophil NADPH oxidase function / T.L. Leto ... [et al.] -- Cell-free assays: the reductionist approach to the study of NADPH oxidase assembly, or "all you wanted to know about cell-free assays but did not dare to ask" / S. Molshanski-Mor ... [et al.] -- Immunoaffinity purification of human phagocyte flavocytochrome b and analysis of conformational dynamics / R.M. Taylor and A.J. Jesaitis -- Genome-scale transcript analyses in human neutrophils / S.D. Kobayashi, D.E. Sturdevant and F.R. DeLeo -- Fast and accurate quantitative analysis of cytokine gene expression in human neutrophils by reverse transcription real-time PCR / N. Tamassia, M.A. Cassatella and F. Bazzoni -- Detection of intact transcription factors in human neutrophils / P.P. McDonald and R.D. Ye -- Disorders of neutrophil function: an overview / M.C. Dinauer -- Diagnostic assays for chronic granulomatous disease and other neutrophil disorders / H.Z. Elloumi and S.M. Holland -- Diagnostic assays for myeloperoxidase deficiency / W.M. Nauseef.
  • Gene Expression Profiling and Regulatory Networks in Single Cells / Fumio Arai, Kentaro Hosokawa, Yoshiko Matsumoto, Hirofumi Toyama and Toshio Suda -- Cell Fate Regulatory Networks / Sonya J. Ridden and Ben D. MacArthur -- Noise in Regulatory Networks / Srinandan Dasmahapatra -- Generalised Modelling in Systems Biology / Thilo Gross -- Relative Quantification Mass Spectrometry Using iTRAQ Isobaric Tags / Richard D. Unwin, Emma Carrick and Anthony D. Whetton -- Deciphering Protein Complexes and Protein Interaction Networks for Stem Cell Pluripotency / Jianlong Wang -- Omics Era in Stem Cell Research: Data Integration of Multi-regulatory Layers / Ana Sevilla, Benjamin Trinite and Ihor R. Lemischka -- High Throughput Affinity Purification and Mass Spectrometry to Determine Protein Complex Interactions / Anna Malovannaya, Rainer B. Lanz, Bert W. O'Malley and Jun Qin -- Network Pharmacology to Aid the Drug Discovery Process / Ruth Dannenfelser, Huilei Xu, Catherine Raimond and Avi Ma'ayan -- What Can Networks Do for You? / Bernhard M. Schuldt, Franz-Josef Müller and Andreas A. Schuppert.
  • Hippocampal slice cultures / Förster, E., Bartos, M., Zhao, S. -- Protocol for the primary culture of cortical neurons / Gonthier, B. ... [et al.] -- A new technique to control the architecture of neuronal networks in vitro / Wyart, C. ... [et al.] -- Organotypic cultures of the rat retina / Hofmann, H.-D. ... [et al.] -- Cerebellar slice cultures / Kapfhammer, J.P. -- Culture of dissociated hippocampal neurons / Rigato, F. -- Improved methods for culturing rat and mouse motor neurons. Their applications / Guettier-Sigrist, S. ... [et al.] -- Characterization of long-term cultured cell lines of murine microglia called MAMI. The case for MAMI-96 cell line / Laporte, V. ... [et al.].
  • Also available: Print – 2002
  • Introduction / Muller Gabbri -- Biogenesis and physiology of microRNAs / Carlos A. Melo and Sonia A. Melo -- Detecting noncoding RNA expression : from arrays to next-generation sequencing / Catalina Perdomo, Joshua Campbell, and Frank Schembri -- MicroRNAs in solid tumors / Jin Wang and Subrata Sen -- MicroRNAs in hematologic malignancies / Johannes Bloehdorn ... [et al.] -- miRNAs as cancer biomarkers / Edward R. Sauter -- Unraveling the complex network of interactions between noncoding RNAs and epigenetics in cancer / Veronica Davalos and Manel Esteller -- MicroRNA SNPs in cancer / Yujing LI and Peng Jin -- Bioinformatics approaches to the study of MircoRNAs / Ravi Gupta and Ramana V. Davuluri -- Beyond miRNAs : role of other noncoding RNAs in cancer / Roxana S. Redis and George A. Calin -- Translational implication for noncoding RNA in cancer / Duaa Dakhallah, Melissa Piper, and S. Patrick Nana-Sinkam.
  • Introduction -- Engineered ionized side chains -- Cysteine modification: probing channel structure, function, and conformational change -- Functional site-directed fluorometry -- Bioreactive tethers -- Flipping the photoswitch: ion channels under light control -- Incorporation of non-canonical amino acids -- Index.
  • Part 1. Basic Science and Experimental Studies on Stem Cell Mobilization -- Preclinical Experience with AMD3100 for Mobilization of Hematopoietic Stem and Progenitor Cells -- Molecular Pharmacology of CXCR4 Inhibition -- Combination Strategies for Stem Cell Mobilization Targeting CXCR4 and S1P Receptors -- Should We Stay or Should We Go Now? -- Cell Adhesion and Mobilization Pathways of Hematopoietic Cells -- Role of the CXCR4/CXCL12 Axis in Hematopoietic Stem Cell Trafficking -- Part 2. Clinical Studies on Stem Cell Mobilization -- Front matter -- Early Studies of AMD3100/Plerixafor in Healthy Volunteers -- The Current Role of Plerixafor in Stem Cell Mobilization for Hematopoietic Stem Cell Transplantation -- Experience with Apheresis Procedures After Plerixafor Mobilisation -- Influence of Different Stem Cell Mobilization Strategies on Graft Composition and Outcome of Autologous or Allogeneic Transplantation -- Relevance and Clinical Implications of Tumor Cell Mobilization in Autologous Transplantation of Multiple Myeloma and Non-Hodgkin's Lymphoma -- Plerixafor: Data from the Compassionate Use Program -- Stem Cell Mobilization with Chemomobilization Plus Plerixafor in Hard-to-Mobilize Patients: The Experience at Kuopio University Hospital, Finland -- Blood Stem Cell Mobilization in Solid Tumors: Experience with Plerixafor and Alternative Ways of Hematopoietic Stem Cell Mobilization -- Mobilization with Chemotherapy + G-CSF + Plerixafor in Europe: The Italian Experience -- Experience with Plerixafor in Poor Mobilizers -- CXCR4 Antagonists -- Part 3. CXCR4 Antagonists for Treatment of Leukemias and Solid Tumors -- Front matter -- Application of CXCR4 Inhibitors in Leukemia -- CXCR4 Antagonists for the Treatment of CML -- Targeting CXCR4 in Chronic Lymphocytic Leukemia: Preclinical Rationale and Early Clinical Experience -- CXCR4-Blockade for Treatment of Breast Cancer -- Molecular Imaging of CXCR4 Receptor Expression in Tumors -- Part 4. Novel applications -- Potential Use of CXCR4 Antagonists to Mobilize Endothelial and Mesenchymal Stem Cells -- Strategies to Enhance Hematopoietic Stem Cell Engraftment Following Transplantation -- Mobilization for Gene Therapy.
  • Also available: Print – 2005
  • Section A. Cell Nucleus: Organization & MechanoBiology -- Chapter 1. Fluorescence Fluctuation Microscopy to Reveal 3D Architecture and Function in the Cell Nucleus / Thorsten Lenser, Klaus Weisshart, Tobias Ulbricht, Karolin Klement, Peter Hemmerich -- Chapter 2. Studying Histone Modifications and Their Genomic Functions by Employing Chromatin Immunoprecipitation and Immunoblotting / Ranveer S. Jayani, Praveena L. Ramanujam, Sanjeev Galande -- Chapter 3. Dynamic Organization of Chromatin Assembly and Transcription Factories in Living Cells / Bidisha Sinha, Dipanjan Bhattacharya, Deepak Kumar Sinha, Shefali Talwar, Shovamayee Maharana, Soumya Gupta, G.V. Shivashankar -- Chapter 4. Manipulation and Isolation of Single Cells and Nuclei / Swee Jin Tan, Qingsen Li, Chwee Teck Lim -- Chapter 5. Beyond Lamins: Other Structural Components of the Nucleoskeleton / Zhixia Zhong, Katherine L. Wilson, Kris Noel Dahl -- Chapter 6. Altered Mechanical Properties of the Nucleus in Disease / Maria Lucia Lombardi, Jan Lammerding -- Chapter 7. Theoretical Concepts and Models of Cellular Mechanosensing / Rumi De, Assaf Zemel, Samuel A. Safran -- Section B. Impact of Nuclear Mechanics on Function -- Chapter 8. Mechanical Induction of Gene Expression in Connective Tissue Cells / Matthew W.C. Chan, Boris Hinz, Christopher A. McCulloch -- Chapter 9. Physical Plasticity of the Nucleus and its Manipulation / Irena Ivanovska, Joe Swift, Takamasa Harada, J. David Pajerowski, Dennis E. Discher -- Chapter 10. Prestressed Nuclear Organization in Living Cells / Aprotim Mazumder, T. Roopa, Abhishek Kumar, K. Venkatesan Iyer, Nisha M. Ramdas, G.V. Shivashankar -- Chapter 11. Nanotopography/Mechanical Induction of Stem-Cell Differentiation / Benjamin Kim Kiat Teo, Soneela Ankam, Lesley Y. Chan, Evelyn K.F. Yim -- Chapter 12. Mechanical Induction in Embryonic Development and Tumor Growth: Integrative Cues Through Molecular to Multicellular Interplay and Evolutionary Perspectives / Maria-Elena Fernandez-Sanchez, Fanny Serman, Padra Ahmadi, Emmanuel Farge -- Chapter 13. Informatics-Based Analysis of Mechanosignaling in the Laminopathies / Frank P.L. Lai, Radfidah A. Mutalif, Siew Cheng Phua, Colin L. Stewart -- Chapter 14. Autosomal Dominant Leukodystrophy Caused by Lamin B1 Duplications: A Clinical and Molecular Case Study of Altered Nuclear Function and Disease / Quasar Saleem Padiath, Ying-Hui Fu.
  • Volume 122 of Methods in Cell Biology describes modern tools and techniques used to study nuclear pore complexes and nucleocytoplasmic transport in diverse eukaryotic model systems (including mammalian cells, Xenopus, C. elegans, yeast). The volume enables investigators to analyze nuclear pore complex structure, assembly, and dynamics; to evaluate protein and RNA trafficking through the nuclear envelope; and to design in vivo or in vitro assays appropriate to their research needs.
  • Nuclear receptors : one big family / Iain J. McEwan -- Methods for measuring ligand dissociation and nuclear receptor turnover in whole cells / Elizabeth M. Wilson -- Flow cytometry as a tool for measurement of steroid hormone receptor protein expression in leukocytes / Cherie L. Butts and Esther M. Sternberg -- X-ray crystallography of agonis/antagonist-bound receptors / Ashley C.W. Pike -- FRAP and FRET methods to study nuclear receptors in living cells / Martin E. van Royen ... [et al.] -- Receptor-DNA interactions : EMSA and footprinting / Jason T. Read ... [et al.] -- Chromatin immunoprecipitation (ChIP) methodology and readouts / Charles E. Massie and Ian G. Mills -- Yeast-based reporter assays for the functional characterization of cochaperone interactions with steroid hormone receptors / Heather A. Balsiger and Marc B. Cox -- High throughput analysis of nuclear receptor-cofactor interactions / Michael L. Goodson, Behnom Farboud, and Martin L. Privalsky -- Binding affinity and kinetic analysis of nuclear receptor/co-regulator interactions using surface plasmon resonance / Derek N. Lavery -- Using RNA interference to study protein function / Carol D. Curtis and Ann M. Nardulli -- Using intrinsic fluorescence emission spectroscopy to study steroid receptor and coactivator protein conformation dynamics / Kate Watt and Iain J. McEwan -- Development of phosphorylation site-specific antibodies to nuclear receptors / Inés Pineda Torra ... [et al.] -- Tissue-selective knockouts of steroid receptors : a novel paradigm in the study of steroid action / Karel De Gendt and Guido Verhoeven -- Methods for identifying and studying genetic alterations in hormone-dependent cancers / Outi R. Saramäki, Kati K. Waltering, and Tapio Visakorpi.
  • Nuclear receptors an introductory overview / C.M. Bunce and M.J. Campbell -- What does evolution teach us about nuclear receptors? / G. Markov, F. Bonneton and V. Laudet -- Functions of nuclear receptors in insect development / D. Martín -- The glucocorticoid receptor / R.H. Oakley and J.A. Cidlowski -- Estrogen receptors: Their actions and functional roles in health and disease / S. Nilsson and J.-Å. Gustafsson -- Androgen receptor / J.T. Dalton and W. Gao -- Thyroid hormone receptors / B. Vennström, H. Liu and D. Forrest -- The vitamin D receptor (NR1I1) / O. Maguire and M.J. Campbell -- Retinoic acid receptors / A. Cras, F. Guidez and C. Chomienne -- PPARs: Important regulators in metabolism and inflammation / L.M. Sanderson and S. Kersten -- Xenobiotic receptors CAR and PXR / C. Klaassen and H. Lu -- Fxr / Y. Wang ... [et al.] -- Physiological functions of TR2 and TR4 orphan nuclear receptor / S. Liu ... [et al.] -- Nuclear receptors and ATP dependent chromatin remodeling: A complex story / C.J. Burd and T.K. Archer -- Non-genomic action of sex steroid hormones / A. Migliaccio, G. Castoria and F. Auricchio -- Ligand regulation and nuclear receptor action / M. Hewison -- New insights to nuclear receptor gene regulation from analysis of their response elements in target genes / C. Carlberg -- Systems biology: Towards realistic and useful models of molecular networks / F.J. Bruggeman ... [et al.].
  • Nuclear transplantation in Xenopus / John B. Gurdon -- Nuclear transfer in sheep / William A. Ritchie -- Protocols for nuclear transfer in mice / Shaorong Gao -- Isolation of stromal stem cells from human adipose tissue / Andrew C. Boquest ... [et al.] -- Nuclear reprogramming by cell fusion / Robert H. Broyles ... [et al.] -- Polyethylene glycol-mediated cell fusion / Jian Yang and Ming Hong Shen -- Epigenetic reprogramming of somatic genomes by electrofusion with embryonic stem cells / Masako Tada and Takashi Tada -- Quantification of cell fusion by flow cytometry / Stephen Sullivan ... [et al.] -- Modulation of cell fate using nuclear and cytoplasmic extracts / Anne-Mari Håkelien, Kristine G. Gaustad, and Philippe Collas -- Transgenic systems in nuclear reprogramming / Megan Munsie, Peter Mountford, and Jennifer Nichols -- Using immunofluorescence to observe methylation changes in mammalian preimplantation embryos / Fátima Santos and Wendy Dean -- Observing S-phase dynamics of histone modifications with fluorescently labeled antibodies / Rong Wu, Anna V. Terry, and David M. Gilbert -- Quantitative analysis of telomerase activity and telomere length in domestic animal clones / Dean H. Betts ... [et al.] -- Pluripotency: capacity for in vitro differentiation of undifferentiated embryonic stem cells / Cornelia Wiese ... [et al.] -- Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens / Bruce A. Fenderson ... [et al.] -- Analysis of the nucleolar compartment of the nucleus as an indicator of nuclear reprogramming after nuclear transfer / Jacques-E. Fléchon -- Methylation-sensitive polymerase chain reaction / Hannah R. Moore, Richard R. Meehan, and Lorraine E. Young -- Analysis of DNA methylation profiles in preimplantation embryos using bisulfite mutagenesis / Yong-Mahn Han, Seok-Ho Kim, and Yong-Kook Kang -- Chromatin immunoprecipitation assay for mammalian tissues / Fiona B. Turner, Wang L. Cheung, and Peter Cheung -- Histone modifications and transcription factor binding on chromatin: ChIP/PCR assays / Jaejoon Won and Tae Kook Kim -- In vivo genomic footprinting using LM-PCR methods / Hiromi Tagoh, Peter N. Cockerill, and Constanze Bonifer -- Analyzing histone modification using crosslinked chromatin treated with micrococcal nuclease / Pascal Lefevre and Constanze Bonifer.
  • Annotation Research into the field of stem cell biology has developed exponentially over recent years, and is beginning to offer significant promise for unravelling the molecular basis of a multitude of disease states. Importantly, in addition to offering the opportunity to delve deeply into the mechanisms that drive disease aetiology the research is realistically opening the doors for development of targeted and personalized therapeutic applications that many considered, until recently, to be nothing more that a far fetched dream. This volume provides a timely glimpse into the methods that have been developed to instigate, and the mechanisms that have been identified to drive, the process of nuclear reprogramming, chronicling how the field has developed over the last 50-60 years. Since the early 1950s a small number of notable experiments have provided significant impetus to the field, primarily the demonstration of reprogramming ability, first by the complex cytoplasmic milieu that constitutes the amphibian egg, then that of the mammalian egg, and finally that of the mammalian embryonic stem cell. Most recently, the demonstration that a limited pool of defined molecules is capable of reprogramming a multitude of cell types has provided massive impetus and facilitated transition towards realistic therapeutic application. We have therefore reproduced some of the key articles that elegantly document these dramatic stages of development of the field in an inclusive appendix to the book, for the benefit of readers keen to investigate the history of how the field of stem cell biology has evolved. Owing to the ever broadening nature of this field, and the incredible rate at which it is evolving, the main content of this volume focuses on areas that have shown significant movement in recent years, are most likely to translate into personalized therapeutic application, and thus provide greatest potential for significant impact on human health in the not too distant future. We recognize that research into many other disease states and cell types are all equally worthy of discussion. We would therefore like to acknowledge those researchers involved whose work we have not been able to include in this volume. Nuclear Reprogramming and Stem Cells will serve as a valuable resource for all researchers in the field of stem cell biology, including those just setting out on their career path as well as those already established in the field.
  • Cloning of exotic/endangered species: (desert bighorn sheep) / James "Buck" Williams ... [et al.] -- Handmade somatic cell cloning in cattle / Gábor Vatja, Ian M. Lewis, and R. Tayfur Tecirlioglu -- Genetic modification of sheep by nuclear transfer with gene-targeted somatic cells / A. John Clark, Sarah Burl, and Chris Denning -- Transgenesis using nuclear transfer in goats / Anthoula Lazaris ... [et al.] -- Nuclear reprogramming : an overview / Takashi Tada -- Expression of imprinted genes in cloned mice / Takashi Kohda, Fumitoshi Ishino, and Atsuo Ogura -- Nuclear remodeling assay in xenopus egg extract / Koichi Gonda and Nobuaki Kikyo -- In vitro reprogramming of nuclei and cells / Anne-Mari Håkelien ... [et al.] -- Techniques for nuclear transfer to mouse embryonic stem cells / Danielè Pralong and Paul J. Verma -- Gene expression and methylation patterns in cloned embryos / Christine Wrenzycki ... [et al.] -- Are there any normal clones? / Ian Wilmut -- Future and applications of cloning / Alan O. Trounson. Origin and progress of nuclear transfer in nonmammalian animals / Marie A. Di Berardino -- In vitro maturation and embryo production in cattle / Marc-André Sirard and Karine Coenen -- Activation of oocytes after nuclear transfer / Zoltán Macháty -- Culture medium preferences of pre-implantation cloned mouse embryos / Björn Heindryckx, Josiane Van der Elst, and Marc Dhont -- Maintenance of pregnancy in pigs with limited viable embryos / Tim King and Paul A. De Sousa -- Health and neonatal care of bovine clones / Jacqui T. Forsyth and David N. Wells -- Optimization of procedures for cloning by somatic cell nuclear transfer in mice / Young Gie Chung, Shaorong Gao, and Keith E. Latham -- Production of cloned calves using roscovitine-treated adult somatic cells as donors / Kazuchika Miyoshi, Sezen Arat, and Steven L. Stice -- A double nuclear transfer technique for cloning pigs / Irina A. Polejaeva, Shawn Walker, and Keith Campbell -- Nuclear transfer in nonhuman primates / Shoukhrat M. Mitalipov and Don P. Wolf --
  • This volume on nucleic acid nanotechnology offers authoritative, up-to-date and comprehensive coverage of nanotechnological studies and applications of nucleic acids. It provides reviews of various aspects of nucleic acid nanotechnology, each written by an internationally leading expert in the field, and presents state-of-the-art and recent advances in nucleic acid synthetic modifications, nanoscale design, manipulation and current and future applications in bioengineering, medicine, electronics, genetic analysis, chemistry, molecular biology, surface and material sciences. It examines how nucleic acid research is merging with nanotechnology, allowing the nanoscale properties of nucleic acid to be exploited in performing challenging nanotechnological tasks, from nanorobotics and nanosensing to nucleic acid computing. This book will above all benefit anyone who is interested in nanotechnological concepts of nucleic acid design and applications, and offers a valuable resource for teaching these concepts. It is essential reading for a broad audience of scientists both in academia and industry who wish to expand their expertise on the potential of nucleic acid functions and applications.
  • This book provides a review of the multitude of nucleic acid polymerases, including DNA and RNA polymerases from Archea, Bacteria and Eukaryota, mitochondrial and viral polymerases, and other specialized polymerases such as telomerase, template-independent terminal nucleotidyl transferase and RNA self-replication ribozyme. Although many books cover several different types of polymerases, no book so far has attempted to catalog all nucleic acid polymerases. The goal of this book is to be the top reference work for postgraduate students, postdocs, and principle investigators who study polymerase.
  • Nucleolus 2011
  • Also available: Print – 2005
  • Also available: Print – 2013
    Fertility preservation has become one of the most important and fast growing fields of reproductive medicine. Although there are several strategies for fertility preservation in female, most of them are still considered experimental. It is important to perfect the existing technologies, but also developing new strategies should be actively sought. The future development of fertility preservation strategies should be based on the sound scientific knowledge and principles. One of the main objectives of fertility preservation in females is prevention of oocyte depletion. The mechanisms of oocyte loss and survival in the ovary are complex, which include genetic control both in germ cells and in somatic cells, DNA damage and repair mechanism, apoptosis and autophagy, and other poorly understood molecular mechanisms.To develop clinically effective and safe strategies for fertility preservation, it is essential to know and understand the fundamentals of oocyte and ovarian biology at the molecular level. Thus, the purpose of this edition is to review the current progress in research related to molecular and genetic control of oocyte development that can be applied to fertility preservation. The main topics that are discussed in this publication include molecular signaling mechanisms of oocyte activation and loss, genomic integrity of oocytes, and epigenetics.
  • Oogenesis 2013
    The oocyte is the largest and most mysterious cell in the body of mammalian organisms. Through its growth and maturation, it reaches extreme levels of specialization, while maintaining at the same time a condition of totipotency. Its unique ability, in co-operation with the spermatozoon, to give rise to a fully developed organism formed from hundreds of different tissues and myriads of individual cells has inspired intellectuals of all ages.Oogenesis finds impetus and purpose in casting scientific perspective towards this unique cell for the benefit of scientists and assisted reproductive technology (ART) specialists. The authors of the chapters are distinguished authorities in their respective areas of competence. From the opening of the book the reader is lead on a fantastic voyage from the formation of the primordial oocyte to the development of the early embryo, passing through crucial processes of oogenesis, such as co-ordination of oocyte and follicle growth, gene expression and organelle reorganization during growth and maturation, epigenetic mechanisms, regulation of meiosis, totipotency, cell polarity, oogenesis in vitro and maternal regulation of early development.
  • Fundamentals of optical microscopy / Franco Quercioli -- The white confocal: continuous spectral tuning in excitation and emission / Rolf Borlinghaus -- Second/third harmonic generation microscopy / Shakil Rehman ... [et al.] -- Role of scattering and nonlinear effects in the illumination and the photobleaching distribution profiles / Zeno Lavagnino, Francesca Cella Zanacchi and Alberto Diaspro -- New analytical tools for evaluation of spherical aberration in optical microscopy / Isabel Escobar ... [et al.] -- Improving image formation by pushing the signal-to-noise ratio / Emiliano Ronzitti ... [et al.] -- Site-specific labeling of proteins in living cells using synthetic fluorescent dyes / Gertrude Bunt -- Imaging molecular physiology in cells using FRET-based fluorescent nanosensors / Fred S. Wouters -- Measuring molecular dynamics by FRAP, FCS, and SPT / Kevin Braeckmans ... [et al.] -- In vitro-in vivo fluctuation spectroscopies / M. Collini ... [et al.] -- Interference x-ray diffraction from single muscle cells reveals the molecular basis of muscle braking / L. Fusi ... [et al.] -- Low concentration protein detection using novel SERS devices / Gobind Das ... [et al.] -- Near infrared three-dimensional nonlinear optical monitoring of stem cell differentiation / Uday K. Tirlapur and Clarence Yapp -- A correlative microscopy: a combination of light and electron microscopy / Umberto Fascio and Anna Sartori-Rupp.
  • Optical microendoscopy is an emerging modality for imaging in live subjects. Using gradient refractive index (GRIN) microlenses, microendoscopy enables subcellular-resolution imaging in deep tissues that are inaccessible by traditional imaging techniques. We present a platform of methods and technologies that build upon GRIN microendoscopy: 1) miniaturized microscopes for imaging in awake, behaving animals, 2) methods for imaging contractile dynamics in the muscles of animal and human subjects, 3) chronic brain preparations that allow for longitudinal examinations of subcellular neuronal features and disease progression, and 4) novel microendoscope probes whose imaging capabilities approach that of standard water-immersion microscope objectives. When combined with the broad sets of available fluorescent reporters, and minimally invasive surgical preparations, the work described in this dissertation enables sophisticated experimental designs for probing how cellular characteristics may underlie or explain behavior, in models of both healthy and diseased states.
  • Oral Cytology: A Concise Guide provides a state-of-the-art review of the major issues specific to the field of oral cytology. The sections of the book have been structured to review the complexities of sample collection, manual/automated analysis, pitfalls, gray zones and controversies in diagnosis, as well as, issues not covered by other available materials. Recent advancements in ancillary techniques like DNA ploidy, image analysis and molecular biology are discussed in detail. This useful text is extensively illustrated with patient and microscopic pictures, which will further highlight the clinical application. Throughout the text, simultaneous inclusion of both cytologic and histologic images will allow cyto-histological correlation. Readers will find descriptions of techniques offering greater simplicity, speed, patient comfort and cost effectiveness, as well as improved diagnostic precision. This bench-side reference is a unique and valuable resource in the field of oral cytology, both for those currently in training and clinicians dealing with the diagnosis of oral lesions.
  • 2012 ClinicalKey
    Chapter 1 Introduction -- Chapter 2 The techniques of FNA cytology -- Chapter 3 Imaging methods for guidance of aspiration cytology -- Chapter 4 Head and neck; salivary glands -- Chapter 5 Lymph nodes -- Chapter 6 The thyroid gland -- Chapter 7 Breast -- Chapter 8 Lung, chest wall and pleura -- Chapter 9 Mediastinum -- Chapter 10 Liver and spleen -- Chapter 11 Pancreas, biliary tract and intra-abdominal organs -- Chapter 12 Kidney, adrenal and retroperitoneum -- Chapter 13 Male and female genital organs -- Chapter 14 Skin and subcutis -- Chapter 15 Soft tissues -- Chapter 16 Bone and cartilage -- Chapter 17 Paediatric tumors -- Chapter 18 Infectious Diseases.
  • Also available: Print – 2008
  • Also available: Print – 2007
    Water compartments in cells / G. D. Fullerton ... [et al.] -- Yeast osmoregulation / S. Hohmann ... [et al.] -- Physiological analysis of bacterial mechanosensitive channels / I. R. Booth ... [et al.] -- Control of cell cycle in response to osmostress: Lessons from yeast / J. Clotet ... [et al.] -- Bacterial osmosensing transporters / J. M. Wood -- Plant gene networks in osmotic stress response: From genes to regulatory networks / L. S. Tran ... [et al.] -- Osmosensing by integrins in rat liver / F. Schliess ... [et al.] -- Hyperosmotic activation of the CD95 system / R. Reinehr ... [et al.] -- New approaches for determining apoptotic volume decrease in cells / C. D. Bortner ... [et al.] -- Transient receptor potential channels in mechanosensing and cell volume regulation / S. F. Pedersen ... [et al.] -- Cell volume regulatory ion channels in cell proliferation and cell death / F. Lang ... [et al.] -- Actin cytoskeleton architecture and signaling in osmosensing / E. A. Papakonstanti ... [et al.] -- Osmotic stress and DNA damage / N. I. Dmitrieva ... [et al.] -- Transcriptional activator tone-binding protein in cellular protection and differentiation / J. A. Kim ... [et al.] -- Desiccation response of mammalian cells: Anhydrosignaling / Z. Huang ... [et al.] -- Tonicity-regulated gene expression / J. D. Ferraris ... [et al.] -- Hyperosmotic induction of mitogen-activated protein kinase scaffolding / T. L. Hilder ... [et al.] -- Osmoregulation of bile formation / R. Kubitz ... [et al.] -- Osmosignaling and volume regulation in intestinal epithelial cells / C. H. Lim ... [et al.] -- Osmotic regulation of cellular glucose uptake / P. Gual ... [et al.] -- Effects of osmolytes on protein folding and aggregation in cells / Z. Ignatova ... [et al.] -- Simulations of macromolecules in protective and denaturing osmolytes: Properties of mixed solvent systems and their effects on water and protein structure and dynamics / D. A. Beck ... [et al.] -- Application of the transfer model to understand how naturally occurring osmolytes affect protein stability / M. Auton ... [et al.] -- Mechanisms of high salinity tolerance in plants / N. Tuteja -- Phenotype of the taurine transporter knockout mouse / U. Warskulat ... [et al.] -- Molecular basis of osmolyte effects on protein and metabolites / J. Rosgen -- Methods of changing biopolymer volume fraction and cytoplasmic solute concentrations for in vivo biophysical studies / M. C. Konopka ... [et al.] -- Characterization of plant aquaporins / R. Kaldenhoff ... [et al.].
  • Introduction / Leopold Flohé and J. Robin Harris -- Evolution of the peroxiredoxins / Bernard Knoops, Eléonore Loumaye and Valérie Van Der Eecken -- Structural survey of the peroxiredoxins / P. Andrew Karplus and Andrea Hall -- The catalytic mechanism of peroxiredoxins / Leslie B. Poole -- Kinetics of peroxiredoxins and their role in the decomposition of peroxynitrite / Madia Trujillo ... [et al.] -- The peroxiredoxin repair proteins / Thomas J. Jönsson and W. Todd Lowther -- Peroxiredoxins in bacterial antioxidant defense / James M. Dubbs and Skorn Mongkolsuk -- The NADH oxidase-Prx system in Amphibacillus xylanus / Youichi Niimura -- Peroxiredoxin systems in mycobacteria / Timo Jaeger -- Peroxiredoxin systems of protozoal parasites / Marcel Deponte, Stefan Rahlfs and Katja Becker -- The trypanothione system / Luise R. Krauth-Siegel, Marcelo A. Comini and Tanja Schlecker -- Functions of typical 2-Cys peroxiredoxins in yeast / Brian A. Morgan and Elizabeth A. Veal -- The dual function of plant peroxiredoxins in antioxidant defence and redox signaling / Karl-Josef Dietz -- Mitochondrial peroxiredoxins / Zhenbo Cao, J. Gordon Lindsay and Neil W. Isaacs -- Peroxiredoxins in the lung with emphasis on peroxiredoxin VI / Bruno Schremmer ... [et al.] -- Peroxiredoxins in gametogenesis and embryo development / Isabelle Donnay and Bernard Knoops -- Peroxiredoxins in the central nervous system / Fumiyuki Hattori and Shinzo Oikawa -- Stress-induced peroxiredoxins / Tetsuro Ishii and Toru Yanagawa.
  • Peroxisomes are a class of ubiquitous and dynamic single membrane-bounded cell organelles, devoid of DNA, with an essentially oxidative type of metabolism. In recent years it has become increasingly clear that peroxisomes are involved in a range of important cellular functions in almost all eukaryotic cells. In higher eukaryotes, including humans, peroxisomes catalyze ether phospholipids biosynthesis, fatty acid alpha-oxidation, glyoxylate detoxification, etc, and in humans peroxisomes are associated with several important genetic diseases.
  • Phospholipases generate lipid signaling molecules through their hydrolytic action on phospholipids and are known to regulate function of a variety of cells under normal and diseased conditions. While several physiological, biochemical and molecular techniques have identified key players involved in different disease processes, phospholipases have also emerged as critical players in the pathogenesis of a number of different diseases including cancer and heart disease. In addition, phospholipases are also implicated in such conditions as brain disorder/injury, kidney and immune cell dysfunction. Phospholipases in Health and Disease is a compilation of review articles dedicated to the study of the field with respect to biochemical and molecular mechanisms of normal and abnormal cell function. The wide range of area covered here is of interest to basic research scientists, clinicians and graduate students, who are engaged in studying pathophysiological basis of a variety of diseases. Furthermore, this book highlights the potential of the different phospholipases as therapeutic targets as well as part of prevention strategies. Twenty three articles in this book are organized in four sections that are designed to emphasize the most characterized forms of the phospholipases in mammalian cells. The first section discusses general aspect of phospholipases. Section two covers the role and function of phospholipase A in different pathophysiological conditions. The third section is focussed on phospholipase C which is believed to play a central role in transmembrane signaling. The final section covers phospholipase D which is present in a variety of different cells. The book illustrates that the activation of phospholipases is of fundamental importance in signal transduction affecting cell function. Overall, this book discusses the diverse mechanisms of phospholipase mediated signal transduction in different pathophysiological conditions and raises the possibility of specific forms of phospholipases serving as novel targets for drug development.
  • Plant tissue cultures / Anna Karkonen [and others] -- Computerized molecular modeling of carbohydrates / Alfred D. French and Glenn P. Johnson -- Oligosaccharide mass profiling (OLIMP) of cell wall polysaccharides by MALDI-TOF/MS / Markus Gunl, Florian Kraemaer, and Markus Pauly -- High-voltage paper electrophoresis (HVPE) of cell-wall building blocks and their metabolic precursors / Stephen C. Fry -- Carbohydrate gel electrophoresis / Florence Goubet, Paul Dupree, and Katja Salomon Johansen -- Monoclonal antibodies, carbohydrate-binding modules, and the detection of polysaccharides in plant cell walls / Cecile Herve, Susan E. Marcus, and J. Paul Knox -- Screening and characterization of plant cell walls using carbohydrate microarrays / Iben Sorensen and William G.T. Willats -- Electron tomography and immunogold labelling as tools to analyse de novo assembly of plant cell walls / Marisa S. Otegui -- Analysing cellulose biosynthesis with confocal microscopy / Meera Nair and Seth DeBolt -- Visual mapping of cell wall biosynthesis / Yumiko Sakuragi, Morten H.H. Norholm, and Henrick V. Scheller -- Atomic force microscopy of plant cell walls / Andrew R. Kirby -- Using solid-state 13C NMR spectroscopy to study the molecular organization of primary plant cell walls / Tracey J. Bootten [and others] -- Formation of cellulose-based composites with hemicelluloses and pectins using gluconacetobacter fermentation / Deirdre Mikkelsen and Michael J. Gidley -- Structural proteins of the primary cell wall : extraction, purification, and analysis / Derek T.A. Lamport, Li Tan, and Marcia J. Kieliszewski -- New insights into the control of cell growth / Claudia Blaukopf, Matthaus Z. Krol, and Georg J. Seifert -- Extraction and detection of Arabinogalactan proteins / Zoe A. Popper -- Characterization of plant cell wall proteome using high-throughput screens / Sang-Jik Lee and Jocelyn K.C. Rose -- Knocking out the wall : protocols for gene targeting in Physcomitrella patens / Alison W. Roberts [and others] -- Measuring in vitro extensibility of growing plant cell walls / Daniel J. Cosgrove.
  • This addition to the Springer Brief in Stem Cells series focuses on aspects related to the specific mechanisms that ensure and control pluripotency and cell commitment in domestic animal species. This topic is generating rapidly-increasing interest due to the great potential for domestic animal species to be used as intermediate biomedical models, between the mouse and the human. The Brief addresses why we need large animal models for regenerative medicine. It also describes early embryo development with a careful and specific analysis of the regulatory mechanisms driving cleavage, polarization and genome activation in domestic species. How pluripotency is compartmentalized in domestic species as well as the different aspects that make the derivation of stem cells in domestic species very difficult are also addressed.
  • Podocytopathy 2014
    Cell biology of the podocyte / Jefferson, J.A.; Shankland, S.J. -- Cell cycle and podocyte injury / Hagmann, H.H.; Shankland, S.J.; Brinkkoetter, P.T. -- The podocyte cytoskeleton : key to a functioning glomerulus in health and disease / Faul, C. -- Crosstalk between podocytes and tubular epithelial cells / Leung, J.C.K.; Kar, N.L.; Tang, S.C.W. -- Role of mitochondria in podocyte injury / Yuan, Y.; Huang, S.; Zhang, A. -- Autophagy in podocytes / Zhang, L.; Livingston, M.J.; Chen, J.-K.; Dong, Z. -- PPAR- : signaling in podocyte injury / Wang, W.; Yang, T. -- Podocyte injury in FSGS / Ding, W.Y.; Saleem, M.A. -- Circulating soluble urokinase receptor and focal segmental glomerulosclerosis / Wei, C.; Reiser, J. -- Anti-phospholipase A2 receptor-1 autoantibodies and membranous nephropathy / Beck, L.H. -- Anti-neutral endopeptidase antibodies, autoantibodies, anti-bovine serum albumin antibodies and membranous nephropathy / Ronco, P.; Debiec, H. -- Podocytopathy in diabetes : a metabolic disorder / Merscher, S.; Lenz, O.; Fornoni, A. -- Obesity-related glomerulopathy and podocyte injury / Camici, M. -- Podocyte injury in HIV-associated nephropathy / Mallipattu, S.K.; He, J.C. -- Retinoic acid : a potential pharmacologic approach in the treatment of podocytopathy / Sharma, S.; Mallipattu, S.K.; Zhong, Y.; He, J.C. -- Immunomodulatory drugs and the podocyte / Mathieson, P.W. -- Triptolide protects podocytes from injury / Qin, W.; Liu, Z.-H. -- Fly : a model to study the podocyte / Na, J.; Cagan, R.L. -- Zebrafish models of podocytopathies / Boucher, R.C.; Zhou, W. -- The role of micrornas in podocytes / Shi, S.; Liu, Z.-H. -- An integrative biology approach to study podocyte function / Ju, W.; Hodgin, J.; Jiang, S.; Kretzler, M.
  • Polyamine structure and synthetic analogs / Patrick M. Woster -- Acute increases in intracellular putrescine lead to the increase in steady-state levels of c-fos, c-jun, RING3, and Id-1 mRNAs / Allan A. Ancheta, Leo Hawel III, and Craig V. Byus -- Polyamine-dependent early cellular signals and cell proliferation / Stina M. Oredsson -- Cellular signals mediating growth arrest after polyamine depletion / Jian-Ying Wang -- Role of polyamines in the regulation of chromatin acetylation / Cheryl A. Hobbs and Susan K. Gilmour -- Role of polyamines in regulation of sequence-specific DNA binding activity / Sripriya Venkiteswaran, Thresia Thomas, and T.J. Thomas -- Polyamine metabolism and the hypertrophic heart / Lisa M. Shantz and Emanuele Giordano -- Influence of polyamines on breast cancer biology / Andrea Manni -- Polyamines in regulation of prostatic cell growth / Raymond G. Schipper ... [et al.] -- Polyamines and cytoskeletal regulation during intestinal epithelial restitution / Ramesh M. Ray and Leonard R. Johnson -- Regulation of Kv channel activity and intercellular junctions by polyamines in intestinal epithelial cells / Jaladanki N. Rao and Jian-Ying Wang -- Polyamine block of Kir channels: toward a molecular picture / Harley T. Kurata and Colin G. Nichols -- Regulation of ornithine decarboxylase expression / Lo Persson -- Evidence for a multistep model for eukaryotic polyamine transport / Richard Poulin ... [et al.] -- Bacterial and eukaryotic transport systems / Kazuei Igarashi and Keiko Kashiwagi -- Regulation of S-adenosylmethionine decarboxylase / Colin Hanfrey -- Genetic engineering of polyamine catabolism in transgenic mice and rats / Juhani Jänne ... [et al.]. Polyamines in kidney biology / Joseph Satriano -- Polyamines in pulmonary vascular biology / Jack W. Olson and Mark N. Gillespie -- Recent advances in the understanding of mammalian polyamine catabolism: the regulation and potential role of polyamine catabolism in drug response and disease processes / Robert A. Casero, Jr. ... [et al.] -- Cellular signaling and polyamines in the control of apoptosis in intestinal epithelial cells / Leonard R. Johnson and Ramesh M. Ray -- The role of ornithine decarboxylase in Myc-induced tumorigenesis / Jonas A. Nilsson and John L. Cleveland -- Protective effect of polyamines on NSAID-induced injury and apoptosis / Heather M. Wallace and Alun Hughes -- Role of polyamines in the control of the immune response in the brain / Denis Soulet and Serge Rivest -- Implication of polyamines in apoptosis of immunoresponse cells / Rupesh Chaturvedi and Keith T. Wilson -- Polyamines and cancer / Ajit K. Verma -- Polyamine pools and cancer prevention / Eugene W. Gerner and David E. Stringer --
  • Polyamines-- methods and protocols 2011
    Springer Protocols
    [Publisher-supplied data] Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development. In Polyamines: Methods and Protocols, leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study. The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Polyamines: Methods and Protocols provides a key resource for all scientists pursuing the study of this dynamic and significant aspect of cellular biology.
  • Introduction / Joseph Seckbach, Aharon Oren, and Helga Stan-Lotter -- Foreword / Richard W. Castenholz -- Preface / Koki Horikoshi -- Editors' biographies -- List of authors and their addresses -- Polyextremophiles and the constraints for terrestrial habitability / M.C. Capece et al. -- Life on the edge and astrobiology : who is who in the polyextremophiles world? / J. Seckbach -- The dynamic genomes of acidophiles / F.J. López de Saro et al. -- Two centuries of microbiological research in the Wadi Natrun, Egypt : a model system for the study of the ecology, physiology, and taxonomy of haloalkaliphilic microorganisms / A. Oren -- Adaptation in haloalkaliphiles and natronophilic bacteria / H.L. Banciu and D.Y. Sorokin -- A random biogeochemical walk into three soda lakes of the western USA : with an introduction to a few of their microbial denizens / R.S. Oremland -- Halophilic, acidophilic, and haloacidophilic prokaryotes / H. Minegishi -- Life in magnesium- and calcium-rich hypersaline environments : salt stress by chaotropic ions / A. Oren -- Survival strategies of halophilic oligotrophic and desiccation resistant prokaryotes / H. Stan-Lotter and S. Fendrihan -- Radiation resistance in extremophiles : fending off multiple attacks / K.M. Webb and J. DiRuggiero -- Thermoalkaliphilic microbes / V. Kumar and T. Satyanarayana -- Acido- and thermophilic microorganisms : their features, and the identification of novel enzymes or pathways / Y. Kawarabayasi -- Microbial diversity in acidic high-temperature steam vents / R.L. Weiss Bizzoco and S.T. Kelley -- Left out in the cold : life in cryoenvironments / J. Goordial et al. -- Microbial diversity and enzymes in ikaite columns : a cold and alkaline environment in Greenland / J.K. Vester et al. -- Microbial communities thriving in various ice ecosystems / B. Sattler et al. -- Snow algae : adaptation strategies to survive on snow and ice / T. Leya -- Adaptation of Antarctic freshwater green algae to extreme environments / H. Hu -- Deep subsurface oil reservoirs as poly-extreme habitats for microbial life. A current review / A. Wentzel et al. -- Expanding limits for life to a new dimension : microbial growth at hypergravity / S. Deguchi and K. Horikoshi -- Microbial eukaryotes in marine oxygen minimum zones / W.D. Orsi and V.P. Edgcomb -- Deep hypersaline anoxic basins as model systems for environmental selection of microbial plankton / A. Stock et al. -- Microbial eukaryotes in hypersaline anoxic deep-sea basins / V.P. Edgcomb and W.D. Orsi -- Life at high salt and low oxygen : how do the Halobacteriaceae cope with low oxygen concentrations in their environment? / A. Oren -- Niches and adaptations of polyextremotolerant black fungi / M. Grube et al. -- Polyextremophilic photoautotrophic eukaryotic algae / W. Reisser -- Extremophilic magnetotactic bacteria / C.T. Lefèvre and D.A. Bazylinski -- Tardigrades : an example of multicellular extremophiles / D. Schulze-Makuch and J. Seckbach -- Polyextremophiles : summary and conclusions / J. Chela-Flores.
  • Identifying transcriptional regulatory regions using reporter genes and DNA-protein interactions by chromatin immunoprecipitation / Lezanne Ooi and Ian C. Wood -- Quantitative RT-PCR methods for investigation of low copy potassium channel gene expresssion in native murine arteries / Alex Cheong, Samuel J. Fountain, and David J. Beech -- Cloning of potassium chanel splice variants from tissues and cells / Lie Chen and Michael J. Shipston -- Chemiluminescence assays to investigate membrane expression and clathrin-mediated endocytosis of KATP channels / Andrew J. Smith and Asipu Sivaprasadarao -- Investigation of KATP channel endocytosis by immunofluorescence / Andrew J. Smith and Asipu Sivaprasadarao -- Investigation of KATP channel endocytosis and cell surface density by biotinylation and western blotting / Andrew J. Smith and Asipu Sivaprasadarao -- Lipid microdomains and K+ channel compartmentation : detergent and non-detergent-based methods for the isolation and characterisation of cholesterol-enriched lipid rafts / Laura J. Sampson and Caroline Dart -- Determination of phosphoinositide binding to K+ channel subunits using a protein-lipid overlay assay / Alison M. Thomas and Andrew Tinker -- Protein complex analysis of native brain potassium channels by proteomics / Guillaume Sandoz and Florian Lesage -- Xenopus oocytes as a heterologous expression system for studying ion channels with the patch-clamp technique / Paolo Tammaro, Kenju Shimomura, and Peter Proks -- Whole-cell recording using the perforated patch clamp technique / Jonathan D. Lippiat -- Recording the activity of ATP-sensitive K+ channels in open-cell cell-attached configuration / Andrei I. Tarasov -- Planar patch clamp : advances in electrophysiology / Andrea Brüggemann ... [et al.] -- Analysing steroid modulation of BKCa channels reconstituted into planar lipid bilayers / Heidi de Wet, Jonathan D. Lippiat, and Marcus Allen -- Using bioluminescence resonance energy transfer to measure ion channel assembly / Gina M. Whitaker and Eric A. Accili -- The use of FRET microscopy to elucidate steady state channel conformational rearrangements and G protein interaction with the GIRK channels / Adi Raveh, Inbal Riven, and Eitan Reuveny -- The voltage-clamp fluorometry technique / Chris S. Gandhi and Riccardo Olcese -- Identification of mutations in the Kir6.2 subunit of the KATP channel / Sarah E. Flanagan and Sian Ellard -- Modulation of potassium ion channel proteins utilising antibodies / Mark L. Dallas, Susan A. Deuchars, and Jim Deuchars -- Fluorescence-based Tl+-influx assays as a novel approach for characterization of small-conductance CA²⁺-activated K+ channel modulators / Susanne Jørgensen, Tina H. Johansen, and Tino Dyhring -- Rubidium efflux as a tool for the pharmacological characterisation of compounds with BK channel opening properties / Neil G. McKay, Robert W. Kirby, and Kim Lawson -- Recording hERG potassium currents and assessing the effects of compounds using the whole-cell patch-clamp technique / Ray M. Helliwell.
  • Introduction to informatics / Liron Pantanowitz -- Basic computing / Seung L. Park, Anil V. Parwani, and Liron Pantanowitz -- Networking / Muhammad A. Syed, Anil V. Parwani, and Liron Pantanowitz -- Databases / Seung L. Park, Anil V. Parwani, and Liron Pantanowitz -- Coding / Seung L. Park, Jacqueline Cuda, and Liron Pantanowitz -- Laboratory information systems / Ioan C. Cucoranu, Anil V. Parwani, and Liron Pantanowitz -- Laboratory information system operations and regulations / Ioan C. Cucoranu, Anil V. Parwani, and Liron Pantanowitz -- Reporting / Liron Pantanowitz -- Quality management / Liron Pantanowitz, Luke T. Wiehagen, and R. Marshall Austin -- Barcoding / Ioan C. Cucoranu, Anil V. Parwani, and Liron Pantanowitz -- Informatics projects / Liron Pantanowitz -- Lean Six Sigman / Ioan C. Cucoranu, Anil V. Parwani, and Liron Pantanowitz -- Electronic medical records / Seung L. Park, Anil V. Parwani, and Liron Pantanowitz -- Digital imaging / Milon Amin, Anil V. Parwani, and Liron Pantanowitz -- Automated pap tests / Liron Pantanowitz -- Telecytology / Sara E. Monaco and Liron Pantanowitz -- Cytology online / Walid E. Khalbuss -- Bioinformatics / Somak Roy, Liron Pantanowitz, and Anil V. Parwani -- Research informatics / Somak Roy, Liron Pantanowitz, Anil V. Parwani.
  • Primary cilia 2009
  • Traction forces exerted by adherent cells / by Cynthia A. Reinhart-King and Daniel A. Hammer -- Control of endothelial cell adhesion by mechanotransmission from cytoskeleton to substrate / by Rosalind E. Mott and Brian P. Helmke -- Use of hydrodynamic shear stress to analyze cell adhesion / by David Boettiger -- Cellular tensegrity models and cell-substrate interactions / by Dimitrije Stamenović, Ning Wang, and Donald E. Ingber -- Bond formation during cell compression / by Elena Lomakina and Richard E. Waugh -- Dynamics of the neutrophil surface during emigration from blood / Thomas R. Gaborski and James L. McGrath -- Glycocalyx regulation of cell adhesion / Philippe Robert, Laurent Limozin, Anne-Marie Benoliel, Anne Pierres, and Pierre Bongrand -- Atomistic modeling of protein adsorption to ceramic biomaterials in water: a first step toward realistic simulation of the biomaterials surface in vivo / by Alan H. Goldstein -- Model cell membrane surfaces for measuring receptor-ligand interactions / by Craig D. Blanchette, Timothy V. Ratto, and Marjorie L. Longo -- A flow chamber for capillary networks: leukocyte adhesion in capillary sized, ligand-coated micropipettes / by David F.J. Tees, Prithu Sundd, and Douglas J. Goetz -- Adhesion of flowing neutrophils to model vessel surfaces: constraint and regulation by the local hemodynamic environment / Gerard B. Nash and G. Ed Rainger -- Hydrodynamic interactions between cells on reactive surfaces / Dooyoung Lee and Michael R. King -- Dynamics of platelet aggregation and adhesion to reactive surfaces under flow / Nipa A. Mody and Michael R. King.
  • History of clinical cytogenetics / Steven L. Gersen -- DNA, chromosomes, and cell division / Martha Keagle -- Human chromosome nomenclature: an overview and definition of terms / Avirachan Tharapel -- Basic laboratory procedures / Martha B. Keagle and Steven L. Gersen -- Microscopy / Christopher McAleer -- Quality control and quality assurance / Michael Watson and Steven L. Gersen -- Instrumentation in the cytogenetics laboratory / Steven L. Gersen and Lotte Downey -- Autosomal aneuploidy / Jin-Chen C. Wang -- Structural chromosome rearrangements / Kathleen Kaiser-Rogers and Kathleen Rao -- Sex chromosomes and sex chromosome abnormalities / Cynthia M. Powell -- The cytogenetics of infertility / Linda Marie Randolph -- Prenatal cytogenetics / Linda Marie Randolph -- The cytogenetics of spontaneous abortion / Solveig M.V. Pflueger -- Chromosome instability / Xiao-Xiang Zhang -- Cytogenetics of hematologic neoplasms / Rizwan Naeem -- Cytogenetics of solid tumors / Jonathan Fletcher -- Genomic imprinting and uniparental disomy / Jin-Chen C. Wang -- Fluroescence in situ hybridization (FISH) / Daynna Wolff and Stuart Schwartz -- Fragile X: from cytogenetics to molecular genetics / Patricia Howard-Peebles and Dana Crawford -- Genomic imprinting and uniparental disomy / Jin-Chen C. Wang -- Genetic counseling / Sarah Hutchings Clark.
  • Also available: Print – 2008
    Cell death : history and future / Zahra Zakeri and Richard A. Lockshin -- Caspase mechanisms / Guy S. Salvesen and Stefan J. Riedl -- The mitochondrial death pathway / Anas Chalah and Roya Khosravi-Far -- Apoptotic pathways in tumor progression and therapy / Armelle Melet ... [et al.] -- Therapeutic targeting of death pathways in cancer : mechanisms for activating cell death in cancer cells / Ting-Ting Tan and Eileen White -- Overcoming resistance to apoptosis in cnacer therapy / Peter Hersey, Xu Dong Zhang, and Nizar Mhaidat -- Trail receptors : targets for cancer therapy / Robin C. Humphreys and Wendy Halpern -- Rational design of therapeutics targeting the BCL-2 family : are some cancer cells primed for death but waiting for a final push? / Victoria Del Gaizo Moore and Anthony Letai -- Autophagy and tumor suppression : recent advancesin understanding the link between autophagic cell death pathways and tumor development / Shani Bialik and Adi Kimchi -- Regulation of programmed cell death by the P53 pathway / Kageaki Kuribayashi and Wafik S. El-Deiry -- Regulation of programmed cell death by NF-[kappa]B and its role in tumorigenesis and therapy / Yongjun Fan ... [et al.] -- Targeting proteasomes as therapy in multiple myeloma / Dharminer Chauhan, Teru Hideshima, and Kenneth C. Anderson -- Histone deacetylase inhibitors : mechanisms and clinical significance in cancer : HDAC inhibitor-induced apoptosis / Sharmila Shankar and Rakesh K. Srivastava -- RNA interference and cancer : endogenous pathways and therapeutic approaches / Derek M. Dykxhoorn, Dipanjan Chowdhury, and Judy Lieberman -- Cancer stem cells and impaired apoptosis / Zainab Jagani and Roya Khosravi-Far.
  • Also available: Print – 2008
  • Also available: Print – 2008
  • The recent advances in Programming Somatic Cell (PSC) including induced Pluripotent Stem Cells (iPS) and Induced Neuronal phenotypes (iN), has changed the experimental landscape and opened new possibilities. The advances in PSC have provided an important tool for the study of human neuronal function as well as neurodegenerative and neurodevelopmental diseases in live human neurons in a controlled environment. For example, reprogramming cells from patients with neurological diseases allows the study of molecular pathways particular to specific subtypes of neurons such as dopaminergic neurons in Parkinson.s Disease, Motor neurons for Amyolateral Sclerosis or myelin for Multiple Sclerosis. In addition, because PSC technology allows for the study of human neurons during development, disease-specific pathways can be investigated prior to and during disease onset. Detecting disease-specific molecular signatures in live human brain cells, opens possibilities for early intervention therapies and new diagnostic tools. Importantly, it is now feasible to obtain gene expression profiles from neurons that capture the genetic uniqueness of each patient. Importantly, once the neurological neural phenotype is detected in vitro, the so-called "disease-in-a-dish" approach allows for the screening of drugs that can ameliorate the disease-specific phenotype. New therapeutic drugs could either act on generalized pathways in all patients or be patient-specific and used in a personalized medicine approach. However, there are a number of pressing issues that need to be addressed and resolved before PSC technology can be extensively used for clinically relevant modeling of neurological diseases.
  • Section 1: Biology of Bacteria and Archaea. How We Do, Don't, and Should Look at Bacteria and Bacteriology / Carl R. Woese -- What Is a Prokaryote? / W. Ford Doolittle, Olga Zhaxybayeva -- Prokaryotes and Their Habitats / Hans G. Schlegel, Holger W. Jannasch -- Origin of Life: RNA World Versus Autocatalytic Anabolist / Günter Wächtershäuser -- Morphological and Physiological Diversity / Stephen H. Zinder, Martin Dworkin -- Prokaryote Characterization and Identification / Peter Kämpfer, Stefanie P. Glaeser -- Principles of Enrichment, Isolation, Cultivation, and Preservation of Prokaryotes / Jörg Overmann -- Comparative Genomics / Asli Ismihan Ozen, Tammi Vesth, David W. Ussery -- Defining Taxonomic Ranks / Konstantinos T. Konstantinidis, Erko Stackebrandt -- Population Genetics / Santiago Castillo-Ramírez, Edward J. Feil -- Public Service Collections and Biological Resource Centers of Microorganisms / David Smith, Dagmar Fritze, Erko Stackebrandt -- Repositories for Patented and Safeguarded Material / Zea D. V. L. Mayerhoff, Irene von der Weid, Alessandra B. G. Valladão -- Biotechnology and Applied Microbiology / Eugene Rosenberg -- Genomes and Post-genome Technology / Betania Ferraz Quirino, Cristine Chaves Barreto, Georgios J. Pappas Jr. -- Section 2: Symbiotic Associations. Role of Microorganisms in Adaptation, Development, and Evolution of Animals and Plants: The Hologenome Concept / Eugene Rosenberg, Ilana Zilber-Rosenberg -- Cyanobacterial-Plant Symbioses / David G. Adams, Birgitta Bergman, Sandra A. Nierzwicki-Bauer, Paula S. Duggan -- Root and Stem Nodule Bacteria of Legumes / Michael J. Sadowsky, Peter H. Graham, Masayuki Sugawara -- Symbiotic Associations Between Ciliates and Prokaryotes / Michael Schweikert, Masahiro Fujishima, Hans-Dieter Görtz -- Bacteriocyte-Associated Endosymbionts of Insects / Paul Baumann, Nancy A. Moran, Linda C. Baumann -- Vibrio fisheri: Squid Symbiosis / Eric V. Stabb, Karen L. Visick -- Rumen Symbioses / Itzhak Mizrahi -- Symbiotic Associations Between Termites and Prokaryotes / Andreas Brune -- Marine Chemosynthetic Symbioses / Colleen M. Cavanaugh, Zoe P. McKiness, Irene L. G. Newton, Frank J. Stewart.
  • Section 1: Prokaryotic Communities. Structure and Function of Microbial Communities / David A. Stahl, Jason J. Flowers, Meredith Hullar, Seana Davidson -- Quantitative and Theoretical Microbial Population Biology / Martin F. Polz, William P. Hanage -- Alpine and Arctic Soil Microbial Communities / M. Rhodes, J. Knelman, R. C. Lynch, J. L. Darcy, D. R. Nemergut, S. K. Schmidt -- Plant Rhizosphere Microbial Communities / Dror Minz, Maya Ofek, Yitzhak Hadar -- Tropical Soil Microbial Communities / Andrew Macrae, Rosalie R. R. Coelho, Raquel Peixoto, Alexandre S. Rosado -- Freshwater Microbial Communities / Jakob Pernthaler -- Pelagic Oxygen Minimum Zone Microbial Communities / Osvaldo Ulloa, Jody J. Wright, Lucy Belmar, Steven J. Hallam -- Marine Deep Sediment Microbial Communities / Andreas Teske -- Sea-Ice Microbial Communities / John P. Bowman -- Coral Reef Bacterial Communities / David G. Bourne, Nicole S. Webster -- Section 2: Ecophysiology -- Planktonic Versus Sessile Life of Prokaryotes / Kevin C. Marshall -- The Phototrophic Way of Life / Jörg Overmann, Ferran Garcia-Pichel -- The Anaerobic Way of Life / Ruth A. Schmitz, Rolf Daniel, Uwe Deppenmeier, Gerhard Gottschalk -- The Chemolithotrophic Prokaryotes / Donovan P. Kelly, Ann P. Wood -- Bacterial Behavior / Judith P. Armitage, Kathryn A. Scott -- Prokaryotic Life Cycles / Lawrence J. Shimkets -- Life at High Temperatures / Rainer Jaenicke, Reinhard Sterner -- Life at Low Temperatures / Thilo M. Fuchs, Klaus Neuhaus, Siegfried Scherer -- Life at High Salt Concentrations / Aharon Oren -- Alkaliphilic Prokaryotes / Terry Ann Krulwich, Masahiro Ito -- Syntrophism Among Prokaryotes / Bernhard Schink, Alfons J. M. Stams -- Quorum Sensing / Bonnie L. Bassler, Melissa B. Miller -- Cell-Cell Interactions / Dale Kaiser.
  • Physiology and Biochemistry -- Acetogenic Prokaryotes / Harold L. Drake, Kirsten Küsel, Carola Matthies -- Virulence Strategies of Plant Pathogenic Bacteria / Maeli Melotto, Barbara N. Kunkel -- Oxidation of Inorganic Nitrogen Compounds as an Energy Source / Eberhard Bock, Michael Wagner -- H₂-Metabolizing Prokaryotes / Edward Schwartz, Johannes Fritsch, Bärbel Friedrich -- Hydrocarbon-Oxidizing Bacteria / Eugene Rosenberg -- Lignocellulose-Decomposing Bacteria and Their Enzyme Systems / Edward A. Bayer, Yuval Shoham, Raphael Lamed -- Aerobic Methylotrophic Prokaryotes / Ludmila Chistoserdova, Mary E. Lidstrom -- Dissimilatory Fe(III)- and Mn(IV)-Reducing Prokaryotes / Derek Lovley -- Dissimilatory Sulfate- and Sulfur-Reducing Prokaryotes / Ralf Rabus, Theo A. Hansen, Friedrich Widdel -- Denitrifying Prokaryotes / James P. Shapleigh -- Dinitrogen-Fixing Prokaryotes / Ernesto Ormeño-Orrillo, Mariangela Hungria, Esperanza Martinez-Romero -- Magnetotactic Bacteria / Dennis A. Bazylinski, Christopher T. Lefèvre, Dirk Schüler -- Luminous Bacteria / Paul V. Dunlap, Henryk Urbanczyk -- Halophilic and Haloalkaliphilic Sulfur-Oxidizing Bacteria / Dimitry Y. Sorokin, Horia Banciu, Lesley A. Robertson, J. Gijs Kuenen -- Colorless Sulfur Bacteria / Gerard Muyzer, J. Gijs Kuenen, Lesley A. Robertson -- Bacterial Stress Response / Eliora Z. Ron -- Anaerobic Biodegradation of Hydrocarbons Including Methane / Johann Heider, Karola Schühle -- Physiology and Biochemistry of the Methane-Producing Archaea / Reiner Hedderich, William B. Whitman.
  • Part I. Cell Wall Polymers and Structures of Bacteria -- 1. The Murein Sacculus / Silke Litzinger and Christoph Mayer -- 2. Occurrence, Structure, Chemistry, Genetics, Morphogenesis, and Functions of S-Layers / Paul Messner, Christina Schäffer, Eva-Maria Egelseer, and Uwe B. Sleytr -- 3. Bacterial Polysaccharide Capsules / David Corbett, Thomas Hudson, and Ian S. Roberts -- 4. Lipopolysaccharides / Alba Silipo, Cristina De Castro, Rosa Lanzetta, Michelangelo Parrilli, and Antonio Molinaro -- 5. Structure, Biosynthesis, and Function of Teichoic Acids and Related Cell Wall Glycopolymers in the Gram-positive Cell Envelope / Maren Rautenberg, Thomas Kohler, Guoqing Xia, Emir Kulauzovic, and Andreas Peschel -- 6. Outer Membrane Proteins / Oliver Mirus, Alexander Hahn, and Enrico Schleiff -- Part II. Cell Wall Polymers and Structures of Archaea -- 7. Cell Envelopes of Methanogens / Harald Claus and Helmut König -- 8. The Cell Envelopes of Haloarchaea: Staying in Shape in a World of Salt / Jerry Eichler, Mehtap Abu-Qarn, Zvia Konrad, Hilla Magidovich, Noa Plavner, and Sophie Yurist-Doutsch -- 9. Cell Envelopes of Crenarchaeota and Nanoarchaeota / Reinhard Rachel -- Part III. Biological Activities -- 10. Immunochemistry of the Cell Walls of Methanogenic Archaea: A View from the Past into the Future / Everly Conway de Macario and Alberto J.L. Macario -- 11. Cell Wall Structure and Pathogenicity / T.A. Oelschlaeger, U. Dobrindt, and J. Hacker -- Part IV. Cell Wall Growth and Inhibition -- 12. Cell Wall Targeted Antibiotics / Regine Hakenbeck, Reinhold Brückner, and Bernhard Henrich -- 13. Bacterial Autolysins / Marie-Pierre Chapot-Chartier -- Part V. Cell Wall Interactions -- 14. Prokaryotic Cell-Cell Interaction / Reinhard Wirth -- 15. Adhesion of Bacteria to Protists / Renate Radek -- Part VI. Application of Cell Wall Components -- 16. Prokaryotic Cell Wall Components: Structure and Biochemistry / Uwe B. Sleytr, Eva-Maria Egelseer, Nicola Ilk, Paul Messner, Christina Schäffer, Dietmar Pum, and Bernhard Schuster -- 17. Accumulation of Heavy Metals by Micro-organisms: Biomineralization and Nanocluster Formation / Sonja Selenska-Pobell and Mohamed Merroun.
  • Also available: Print – 2013
  • 1. Delineating protease functions during cancer development / Nesrine I. Affara, Pauline Andreu, and Lisa M. Coussens -- 2. Proteolytic systems: constructing degradomes / Gonzalo R. Ordóñez ... [et al.] -- 3. Microarrays for protease detection in tissues and cells / Kamiar Moin ... [et al.] -- 4. Positional scanning synthetic combinatorial libraries for substrate profiling / Eric L. Schneider and Charles S. Craik -- 5. Mixture-based peptide libraries for identifying protease cleavage motifs / Benjamin E. Turk -- 6. High throughput substrate phage display for protease profiling / Boris Ratnikov, Piotr Cieplak, and Jeffrey W. Smith -- 7. Imaging specific cell surface protease activity in living cells using reengineered bacterial cytotoxins / John P. Hobson ... [et al.] -- 8. Cell-based identification of natural substrates and cleavage-sites for extracellular proteases by SILAC proteomics / Magda Gioia, Leonard J. Foster, and Christopher M. Overall -- 9. Optical proteolytic beacons for in vivo detection of matrix metalloproteinase activity -- J. Oliver McIntyre and Lynn M. Matrisian -- 10. Dissecting the urokinase activation pathway using urokinase-activated anthrax toxin / Shihui Liu ... [et al.] -- 11. On-demand cleavable linkers for radioimmunotherapy / Pappanaicken R. Kumaresan, Juntao Luo, and Kit S. Lam.
  • In view of rapidly growing research in the deregulation of proteases and their impact in human health and diseases, this book will highlight existing and emerging research in this exciting area. In-depth critical state-of-the-art reviews will be written by established investigators on proteases dysfunctions associated with pathogenesis of different diseases that are known to occur due to deregulation of proteolytic systems. Multidisciplinary approaches demonstrating biochemical and signal transduction mechanisms associated with deregulation of proteases leading to manifestation of the diseases.
  • Also available: Print – 2010
    Energetics of peptide and protein binding to lipid membranes / William C. Wimley -- Membrane association and pore formation by alpha-helical peptides / Burkhard Bechinger -- Role of membrane lipids for the activity of pore forming peptides and proteins / Gustavo Fuertes ... [et al.] -- Cholesterol-dependent cytolysins / Robert J.C. Gilbert -- Laetiporus sulphureus lectin and aerolysin protein family / Jose Miguel Manchero .. [et al.] -- Interfacial interactions of pore-forming colicins / Helen Ridley, Christopher L. Johnson and Jeremy H. Lakey -- Permeabilization of the outer mitochondrial membrane by BCL-2 proteins / Ana J. Garcia-Suez ... [et al.] -- Molecular mechanism of sphingomyelin : specific membrane binding and pore formation by actinoporins / Biserka Bakra and Gregor Anderluh -- Hemolysin E (hlye, clya, shea) and related toxins / Stuart Hunt, Jeffrey Green and Peter J. Artymiuk -- Pore formation by cry toxins / Mario Soberin ... [et al.] -- Role of heparan sulfates and glycosphingolipids in the pore formation of basic polypeptides of cobra cardiotoxin / Wen-guey Wu ... [et al.] -- Amyloid peptide pores and the beta sheet conformation / Bruce L. Kagan and Jyothi Thundimadathil.
  • Neurosphere and Neural Colony-Forming Cell Assays / Sharon A. Louis, Brent A. Reynolds -- Directed Neuronal Differentiation of Embryonic and Adult-Derived Neurosphere Cells / Marcos R. Costa, Ravi Jagasia, Benedikt Berninger -- Culture and Differentiation of Human Neural Stem Cells / Soojung Shin, Mohan Vemuri -- Neural Differentiation of Human Embryonic Stem Cells / Mirella Dottori, Alice Pébay, Martin F. Pera -- Isolation and Culture of Primary Human CNS Neural Cells / Manon Blain, Veronique E. Miron, Caroline Lambert, Peter J. Darlington, Qiao-Ling Cui, Philippe Saikali, V. Wee Yong, Jack P. Antel -- Bioengineering Protocols for Neural Precursor Cell Expansion / Behnam A. Baghbaderani, Arindom Sen, Michael S. Kallos, Leo A. Behie -- Intracellular Calcium Assays in Dissociated Primary Cortical Neurons / Navjot Kaur, David V. Thompson, David Judd, David R. Piper, Richard G. Del Mastro -- Dissociated Hippocampal Cultures / Francine Nault, Paul Koninck -- Primary Sensory and Motor Neuron Cultures / Andrea M. Vincent, Eva L. Feldman -- Retinal Cell and Tissue Culture / Francisco L.A.F. Gomes, Michel Cayouette -- Preparation of Normal and Reactive Astrocyte Cultures / Jean Vellis, Cristina A. Ghiani, Ina B. Wanner, Ruth Cole -- Oligodendrocyte Progenitor Cell Culture / Akiko Nishiyama, Ryusuke Suzuki, Hao Zuo, Xiaoqin Zhu -- Isolation of Microglia Subpopulations / Makoto Sawada, Hiromi Suzuki -- Microglia from Progenitor Cells in Mouse Neopallium / Sergey Fedoroff, Arleen Richardson -- Primary Schwann Cell Cultures / Haesun A. Kim, Patrice Maurel -- Primary Dissociated Astrocyte and Neuron Co-culture / Shelley Jacobs, Laurie C. Doering -- Cerebellar Slice Cultures / Josef P. Kapfhammer -- Hippocampal Slice Cultures / Jesse E. Hanson, Adrienne L. Orr, Silvia Fernandez-Illescas, Ricardo A. Valenzuela, Daniel V. Madison -- Molecular Substrates for Growing Neurons in Culture / Saulius Satkauskas, Arnaud Muller, Morgane Roth, Dominique Bagnard -- Guidance and Outgrowth Assays for Embryonic Thalamic Axons / Alexandre Bonnin -- Detection of Cell Death in Neuronal Cultures / Sean P. Cregan -- Live Imaging of Neural Cell Functions / Sabine Bavamian, Eliana Scemes, Paolo Meda -- Tissue Culture Procedures and Tips / Arleen Richardson, Sergey Fedoroff.
  • Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays * Discusses many new assays developed since the previous edition. Presents recent developments in cytometric intrumentation/technology.
  • Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays. Discusses many new assays developed since the previous edition * Presents recent developments in cytometric intrumentation/technology.
  • Also available: Print – 2010
  • This new volume of Methods in Cell Biology looks at receptor-receptor interactions, with sections on allosteric and effector interactions, crystallization and modeling, measuring receptor-receptor interactions and oligomerization in individual classes. With cutting-edge material, this comprehensive collection is intended to guide researchers of receptor-receptor interactions for years to come.
  • Ch. 1. Introduction -- ch. 2. Determinants of red blood cell aggregation -- ch. 3. Mechanism of red blood cell aggregation -- ch. 4. Measurement of red blood cell aggregation -- ch. 5. Effect of red blood cell aggregation on in Vitro blood rheology -- ch. 6. Effect of red blood cell aggregation on tube flow -- ch. 7. In Vivo hemodynamics and red blood cell aggregation -- ch. 8. Alterations in red blood cell aggregation -- ch. 9. Comparative aspects of red blood cell aggregation.
  • Redox cell biology and genetics. Part A-B pt. A-B, 2002.
    pt. B ScienceDirect
    Also available: Print – pt. A-B, 2002.
  • The role of redox in signal transduction / John T. Hancock -- The measurement of nitric oxide and its metabolites in biological samples by ozone-based chemiluminescence / Andrew G. Pinder ... [et al.] -- Detection and measurement of reactive oxygen intermediates in mitocondria and cells / Matthew Whiteman ... [et al.] -- Redox-sensitive green fluorescent protein : probes for dynamic intracellular redox responses : a review / Mark B. Cannon and S. James Remington -- Measuring redox changes in vivo in leaves : prospects and technical challenges / Philip M. Mullineaux and Tracy Lawson -- Imaging of intracellular hydrogen peroxide production with hyper upon stimulation of hela cells with Egf / Kseniya N. Markvicheva ... [et al.] -- Tools to investigate ROS sensitive signalling proteins / Radhika Desikan ... [et al.] -- Methods for preparing crystals of reversibly oxidized proteins : crystallization of protein tyrosine phophatase 1b as an example / Annette Salmeen and David Barford -- Methods for the study of redox-mediated changes in p53 structure and function / Kristine Mann -- Redox regulation and trapping sulphenic acid in the peroxide sensitive human mitochondrial branched chain aminotransferase / Susan M. Hutson ... [et al.] -- Detection of carbonylated proteins in 2-D SDS page separations / Rukhsana Sultana ... [et al.] -- Analysis of global and specific changes in the disulfide proteome using redox 2D-PAGE / Robert C. Cumming -- Protein-thiol oxidation from single proteins to proteome-wide analyses / Natacha Le Moan, Frédérique Tacnet, and Michel B. Toledano -- Analysis of redox relationships in the plant cell cycle : determinations of ascorbate, glutathione, and poly (ADPribose) polymerase (PARP) in plant cell cultures / Christine H. Foyer ... [et al.] -- Generation and detection of S-nitrosothiols / Christian Lindermayr, Simone Sell, and Jörg Durner.
  • Inducing embryonic stem cells to become cardiomyocytes / Alexander M. Becker, Michael Rubart, and Loren J. Field -- Regenerating function in vivo with myocytes derived from embryonic stem cells / Priya R. Baraniak and Todd C. McDevitt -- Excitation-contraction coupling, functional properties, and autonomic and hormonal regulation in human embryonic stem cell derived cardiomyocytes / Oshra Sedan and Ofer Binah -- Embryonic stem cell derivatives for cardiac therapy : advantages, limitations, and long-term prospects / Michal Weiler-Sagie and Lior Gepstein -- Methods for differentiation of bone-marrow-derived stem cells into myocytes / Shinji Makino and Keiichi Fukuda -- Homing, survival, and paracrine effects of human mesenchymal stem cells / Sergey Doronin -- Bone marrow cell therapy after myocardial infarction : what have we learned from the clinical trials and where are we going? / Kai C. Wollert -- Evidence for the existence of resident cardiac stem cells / Isotta Chimenti ... [et al.] -- Multiple sources for cardiac stem cells and their cardiogenic potential / Antonio Paolo Beltrami, Daniela Cesselli, and Carlo Alberto Beltrami -- Skeletal muscle stem cells in the spotlight : the satellite cell / Zipora Yablonka-Reuveni and Kenneth Day -- Regenerating mechanical function in vivo with skeletal myoblasts / Todd K. Rosengart and Muath Bishawi -- Methods for inducing pluripotency / Raymond L. Page, Christopher Malcuit, and Tanja Dominko -- Inducible pluripotent stem cells for cardiac regeneration / Naama Zeevi-Levin and Joseph Itskovitz-Eldor -- Induced pluripotent cells for myocardial infarction repair / Timothy J. Nelson and Andre Terzic -- Substrates of cardiac reentrant arrhythmias : the possible role of tissue regeneration and replacement / André G. Kléber -- Integration of stem cells into the cardiac syncytium : formation of gap junctions / Peter R. Brink, Ira S. Cohen, and Richard T. Mathias -- Bradyarrhythmia therapies : the creation of biological pacemakers and restoring atrioventricular node function / Richard B. Robinson -- Tachyarrhythmia therapies : approaches to atrial fibrillation and postinfarction ventricular arrhythmias / J. Kevin Donahue and Kenneth R. Laurita -- Long-term prospects for arrhythmia treatment : advantages and limitations of gene and cell therapies / Michael R. Rosen -- Regenerating blood vessels / Tracy A. Gwyther and Marsha W. Rolle -- Regenerating heart valves / Benedikt Weber and Simon P. Hoerstrup -- Tissue engineering strategies for cardiac regeneration / Amandine F.G. Godier-Furnémont ... [et al.] -- Methods of cell delivery for cardiac repair / Sarah Fernandes and Hans Reinecke -- Tracking of stem cells in vivo / Yingli Fu and Dara L. Kraitchman -- Assessing regional mechanical function after stem cell delivery / Jacques P. Guyette and Glenn R. Gaudette.
  • This book represents a major contribution to the emerging science of regenerative medicine using non-fetal sources of stem cells. The Editors, Dr Niranjan Bhattacharya and Professor Phillip Stubblefield, have brought together some of the most pre-eminent scientists working on regenerative medicine to share information on currently ongoing work in this area alongside unpublished observations that will help to shape the contours of future therapies. Regenerative Medicine: Using Non-Fetal Sources of Stem Cells discusses the potential clinical and therapeutic applications using non-fetal stem cells as well as providing instruction on the collection, isolation and characterization of stem cells from various non-fetal sources, such as menstrual blood, adipose tissue, breast milk and uprooted decidual teeth. This book will be an invaluable resource for both active researchers and those entering the field. The Editors truly hope that the text will act as a stimulant to professionals and clinical scientists, who may be inspired to further the work of the pioneering scientists who have contributed to this volume.
  • 1. Cell Therapy for Neurodegenerative Disorders / Ilyas Singec -- 2. New Prospects for Neural Stem Cell Therapies of Nervous System Diseases Toward the Establishment of Atypical Ectopic Perivascular Stem Cell Niches / Clara Alfaro-Cervello, Chiara Cossetti, Elena Giusto, Matteo Donegà and Stefano Pluchino -- 3. Cultured Limbal Epithelial Stem Cell Therapy for Ocular Surface Diseases / Hannah J. Levis, Julie T. Daniels and Sajjad Ahmad -- 4. Keratinocyte Stem Cells: Biology and Clinical Applications / Carlo Pincelli and Alessandra Marconi -- 5. Cardiac Regeneration with Stem Cells / Beatriz Pelacho, Manuel Mazo, Sheyla Montori, Ana Maria Simon-Yarza, Juan Jose Gavira, Maria J. Blanco-Prieto and Felipe Prósper -- 6. Development of Biological Approaches to Improve Muscle Healing After Injury and Disease / Jonathan D. Proto and Johnny Huard -- 7 Mesenchymal Stem Cells in Bone and Cartilage Regeneration / Mohamadreza Baghaban Eslaminejad, Elham Zomorodian and Fatemeh Bagheri -- 8. Pancreatic Reprogramming / Juan Domínguez-Bendala -- 9. Pancreatic Regeneration in the Face of Diabetes / Zeeshan Ahmad -- 10. Regenerative Therapies for Liver Diseases / Amar Deep Sharma, Ina Rittelmeyer, Tobias Cantz and Michael Ott -- 11. Clinical Studies of Cell Therapy for Liver Cirrhosis / James A. Thomas and Stuart J. Forbes -- 12. Stem Cell Applications for the Treatment of Gastrointestinal System Diseases / Jacobo Trebol Lopez, Tihomir Georgiev-Hristov, Mariano García-Arranz and Damián García-Olmo -- 13. Germ Line Stem Cells: A Promising Alternative Source for Stem-Cell-Based Therapies in Regenerative Medicine / Ellen Goossens and Herman Tournaye -- 14. Cord Blood Stem Cells and Regenerative Medicine / David T. Harris.
  • A massive wastage of the global resources -- Placenta as a source of stem cells and as a key organ for fetomaternal tolerance -- Placenta and umbilical cord in traditional Chinese medicine -- Use of umbilical venous blood on assessing the biochemical variations of acid-base, nutritional and metabolic parameters on growth-retarded fetuses, in comparison with gestational control cases: a study -- Umbilical cord blood transfusion and its therapeutic potentialities -- Autologous placental blood transfusion for the therapy of anemic neonates -- Cord blood: a massive waste of a life-saving resource: a perspective on its current and potential uses -- Clinical experience of cord blood autologous transfusion -- Emergency use of human cord blood -- Hemoglobin-based oxygen carriers in trauma care: the US multicenter prehospital trial -- Placental umbilical cord blood as a true blood substitute with an edge -- Implications of feto-maternal cell transfer in normal pregnancy -- Early reports on the prognostic implications and immunotherapeutic potentials of Cd34 rich cord whole blood transfusion in advanced breast cancer with severe anemia -- Anti-inflammatory effects of human cord blood and its potential implications in neurological disorders -- Transforming "waste" into gold: identification of novel stem cells resources with therapeutic potential in neuromuscular disorders -- Human umbilical cord blood cells for stroke -- Placental umbilical cord blood transfusion for stem cell therapy in neurological diseases -- Umbilical cord and its blood: a perspective on its current and potential use in ophthalmology -- Umbilical vein grafts for lower limb revascularization -- Cord blood stem cells in angiogenesis. Endothelial progenitor cells from cord blood: magic bullets against ischemia? -- Therapeutic potential of placental umbilical cord blood in cardiology -- Stem cell therapy for heart failure using cord blood -- Human umbilical cord blood mononuclear cells in the treatment of acute myocardial infarction -- Umbilical cord-derived mesenchymal stem cells -- Cord blood stem cell expansion ex vivo: current status and future strategies -- Embryonic-like stem cells and the importance of human umbilical cord blood for regenerative medicine -- Use of non-hematopoietic stem cells of fetal origin from cord blood, umbilical cord, and placenta in regeneration medicine -- Animal studies of cord blood and regeneration -- Immune privilege of cord blood -- Combination cellular therapy for regenerative medicine: the stem cell niche -- Use of cord blood in regenerative medicine -- Comparisons between related and unrelated cord blood collection and/or banking for transplantation or research: the UK NHS blood and transplant experience -- Donor and collection-related variables affecting product quality in ex utero cord blood banking -- Cord blood as a source of hematopoietic progenitors for transplantation -- Amniotic fluid and placenta stem cells -- Use of amniotic membrane, amniotic fluid, and placental dressing in advanced burn patients -- Clinical use of amniotic fluid in osteoarthritis: a source of cell therapy -- A study and follow-up (1999-2009) of human fetal neuronal tissue transplants at a heterotopic site outside the brain in cases of advanced idiopathic Parkinsonism -- Ethical issues surrounding umbilical cord blood donation and banking.
  • Examining the molecular mechanism of Bcl-2 family proteins at membranes by fluorescence spectroscopy -- Photoreactive stapled peptides to identify and characterize BCL-2 family interaction sites by mass spectrometry -- The structural biology of BH3-only proteins -- How to analyze mitochondrial morphology in healthy cells and apoptotic cells in Caenorhabditis elegans -- Apoptosis initiation through the cell-extrinsic pathway -- Using RNAi screening technologies to interrogate the extrinsic apoptosis pathway -- Caspase enzymology and activation mechanisms -- Turning on caspases with genetics and small molecules -- A multipronged approach for compiling a global map of allosteric regulation in the apoptotic caspases -- Measuring caspase activity in vivo -- Single-molecule sensing of caspase activation in live cells via plasmon coupling nanotechnology -- In vivo monitoring of caspase activation using a fluorescence resonance energy transfer-based fluorescent probe -- Global analysis of cullular proteolysis by selective enzymatic labelling of protein N-termini -- Complementary methods for the indentification of substrates of proteolysis -- Phospholipid scrambling on the plasma membrane -- Studying apoptosis in the zebrafish.
  • Reporter genes : a practical guide 2007
    Springer Protocols
    Methodologies for staining and visualization of [beta]-galactosidase in mouse embryos and tissues / Siobhan Loughna and Deborah Henderson -- Immunohistochemical detection of [beta]-galactosidase or green fluorescent protein on tissue sections / Philip A. Seymour and Maike Sander -- Detection of reporter gene expression in murine airways / Maria Limberis, Peter Bell, and James M. Wilson -- Three-dimensional analysis of molecular signals with episcopic imaging techniques / Wolfgang J. Weninger and Timothy J. Mohun -- Fluorescent proteins for cell biology / George H. Patterson -- Detection of GFP during nervous system development in Drosophila melanogaster / Karin Edoff, James S. Dods, and Andrea H. Brand -- Autofluorescent proteins for flow cytometry / Charles G. Bailey and John E. J. Rasko -- Fluorescent protein reporter systems for single-cell measurements / Steven K. Dower, Eva E. Qwarnstrom, and Endre Kiss-Toth -- Subcellular imaging of cancer cells in live mice / Robert M. Hoffman -- Noninvasive imaging of molecular events with bioluminescent reporter genes in living subjects / Pritha Ray and Sanjiv Sam Gambhir -- Green fluorescent protein as a tracer in chimeric tissues : the power of vapor fixation / Harald Jockusch and Daniel Eberhard.
  • Ch. 1. DT40 gene disruptions: a how-to for the design and the construction of targeting vectors / Hiroshi Arakawa and Jean-Marie Buerstedde -- Ch. 2. Immunoglobulin gene conversion or hypermutation: That's the question / Jean-Marie Buerstedde and Hiroshi Arakawa -- Ch. 3. Genome resources for the DT40 community / Randolph B Caldwell and Andrzej M. Kierzek -- Ch. 4. Chromosome engineering in DT40 cells and mammalian centromere function / William R.A. Brown ... [et al.] -- Ch. 5. Function of recQ family helicase in genome stability / Masayuki Seki, Shusuke Tada and Takemi Enomoto -- Ch. 6. Genetic analysis of apoptotic execution / Sandrine Ruchaud ... [et al.] -- Ch. 7. The DT40 system as a tool for analyzing kinetochore assembly / Masahiro Okada, Tesuya Hori and Tatsuo Fukagawa -- Ch. 8. Analysing the DNA damage and replication checkpoints in DT40 cells / Michael D Rainey, George Zachos, and David AF Gillespie -- Ch. 9. Using DT40 to study clathrin function / Frank R. Wettey and Antony P. Jackson -- Ch. 10. Genetic analysis OF B cell signaling / Hisaaki Shinohara and Tomohiro Kurosaki -- Ch. 11. DT40 mutants: a model to study transcriptional regulation of B cell development and function / Jukka Alinikula, Olli Lassila and Kalle-Pekka Nera -- Ch. 12. Transcription and RNA processing factors play complex roles IN DT40 cells / Stephanie Bush and James L. Manley -- Ch. 13. Participation of histones, histone modifying enzymes and histone chaperones in vertebrate cell functions / Hidehiko Kikuchi ... [et al.] -- Ch. 14. Analysis of gene expression, copy number and palindrome formation with a DT40 enriched cDNA Microarray / Paul E. Neima ... [et al.] -- Ch. 15. Analysis of DNA replication damage bypass and its role in immunoglobulin repertoire development / Julian E. Sale, Anna-Laura Ross and Laura J. Simpson -- Ch. 16. The fanconi anemia pathway promotes homologous recombination repair in DT40 cell line / Julian E. Sale, Anna-Laura Ross and Laura J. Simpson -- Ch. 17. Calcium signaling, ion channels and more / Anne-Laure Perraud, Carsten Schmitz, Andrew M. Scharenberg -- Ch. 18. Phenotypic analysis of cellular responses to DNA damage / Helfrid Hochegger and Shunichu Takeda -- Ch. 19. ATM, a paradigm for a stress-responsive signal transducer in higher vertebrate cells / Ken-ichi Yamamoto, Masahiko Kobayashi and Hiroko Shimizu -- METHOD 1. Stable transfection of DT40 / Julian E. Sale -- METHOD 2. Basic cell culture conditions / Huseyin Saribasak and Hiroshi Arakawa -- METHOD 3. Excision of floxed-DNA sequences by transient induction of Mer-Cre-Mer / Hiroshi Arakawa -- METHOD 4. Immunoglobulin gene conversion and hypermutation assay by FACS / Hiroshi Arakawa -- METHOD 5. Target screening by PCR / Hiroshi Arakawa -- METHOD 6. Mitotic index determination by flow cytometry / David AF Gillespie and Mark Walker -- METHOD 7. Centrifugal elutriation as a means of cell cycle phase separation and synchronization / David AF Gillespie and Catarina Henriques -- METHOD 8. Preparation of genomic DNA for microarray-based comparative genome hybridization / Robert Kimmel, Amalia Icreverzi, Paul Neiman -- METHOD 9. Analysis of cellular Mg2+ in DT40 cells / Anne-Laure Perraud, Carsten Schmitz, Andrew M. Scharenberg -- METHOD 10. Transient transfection of DT40 / Roger Franklin & Julian E. Sale -- METHOD 11. Retroviral transduction of DT40 / Felix Randow & Julian E. Sale -- METHOD 12. Colony survival assay / Laura. J. Simpson & Julian E. Sale -- METHOD 13. Subcloning DT40 by limiting dilution / Jean-Marie Buerstedde -- METHOD 14. Subnuclear immunofluorescence / Dávid Szüts & Julian E. Sale -- METHOD 15. Sister chromatid exchange assay / Laura J. Simpson & Julian E. Sale -- METHOD 16. 2D cell cycle analysis / Roger Franklin & Julian E. Sale -- METHOD 17. Purification of tap-tagged proteins by two-step pull down from DT40 cells / Hiroyuki Kitao and Minoru Takata -- METHOD 18. Synchronization of cells / Eiichiro Sonoda -- METHOD 19. Tranfection of DT40 cells / Huseyin Saribasak and Hiroshi Arakawa -- METHOD 20. Luciferase reporter assay / Frank R. Wettey and Antony P. Jackson -- METHOD 21. Indirect immunofluorescence microscopy / Frank R. Wettey and Antony P. Jackson -- METHOD 22. Quantification of receptor-mediated endocytosis / Frank R. Wettey and Antony P. Jackson -- METHOD 23. Measurement of DNA synthesis and strand breaks using alkaline sucrose density gradient centrifugation / Kouichi Yamada and Jun Takezawa -- METHOD 24. Fractionation of nuclear and cytoplasmic proteins from DT40 cell lines / Yan-Dong Wang and Randolph B Caldwell.
  • The ribosome is a macromolecular machine that synthesizes proteins with a high degree of speed and accuracy. Our present understanding of its structure, function and dynamics is the result of six decades of research. This book collects over 40 articles based on the talks presented at the 2010 Ribosome Meeting, held in Orvieto, Italy, covering all facets of the structure and function of the ribosome. New high-resolution crystal structures of functional ribosome complexes and cryo-EM structures of translating ribosomes are presented, while partial reactions of translation are examined in structural and mechanistic detail, featuring translocation as a most dynamic process. Mechanisms of initiation, both in bacterial and eukaryotic systems, translation termination, and novel details of the functions of the respective factors are described. Structure and interactions of the nascent peptide within, and emerging from, the ribosomal peptide exit tunnel are addressed in several articles. Structural and single-molecule studies reveal a picture of the ribosome exhibiting the energy landscape of a processive Brownian machine. The collection provides up-to-date reviews which will serve as a source of essential information for years to come.
  • Also available: Print – 2005
    Mechanisms of apoptosis during reovirus infection / P. Clarke ... [et al.] -- Poliovirus, pathogenesis of poliomyelitis, and apoptosis / B. Blondel ... [et al.] -- Neuronal cell death in alphavirus encephalomyelitis / D. E. Griffin -- HSV-induced apoptosis in herpes encephalitis / L. Aurelian -- The role of apoptosis in defense against baculovirus infection in insects / R. J. Clem -- The role of host cell death in Salmonella infections / D. G. Guiney -- Role of macrophage apoptosis in the pathogenesis of Yersinia / Y. Zhang, J. B. Bliska -- Entamoeba histolytica activates host cell caspases during contact-dependent cell killing / D. R. Boettner, W. A. Petri -- Interactions between malaria and mosquitoes : the role of apoptosis in parasite establishment and vector response to infection / H. Hurd, V. Carter, A. Nacer -- Apoptosis and its modulation during infection with Toxoplasma gondii : molecular mechanisms and role in pathogenesis / C. G. K. Luder, U. Gross -- Modulation of the immune response in the nervous system by rabies virus / M. Lafon -- Apoptotic cells at the crossroads of tolerance and immunity / M. Skoberne ... [et al.].
  • It is now well known that proteases are found everywhere, in viruses and bacteria as well as in all human, animal and plant cells, and play a role in a variety of biological functions ranging from digestion, fertilization, development to senescence and death. Under physiological conditions the ability of proteases is regulated by endogenous inhibitors. However, when the activity of proteases is not regulated appropriately, disease processes can result, as seen in Alzheimer's disease, cancer metastasis and tumor progression, inflammation and atherosclerosis. Thus it is evident that there is an absolute need for a tighter control of proteolytic activities in different cells and tissues. Aimed at graduate students and researchers with an interest in cellular proteolytic events, Role of Proteases in Cellular Dysfunctions is the second book on Proteases in this series. The book consists of three parts in specified topics based on current literatures for a better understanding for the readers with respect to their subject-wise interests. The first section of this book covers a brief idea about the neuronal disorders and the involvement of proteases such as calpains, caspases and matrix metalloproteases (MMPs). The second section covers the deadly disease cancer and its relation to ubiquitin-proteosomal system, MMPs and serine proteases. The last section is about the role of proteases such as calpains, MMPs and serine protease as well as urokinase type plasminogen activator receptor (uPAR) in causing cardiovascular defects.
  • Also available: Print – 2007
    The CRMP family of proteins and their role in Sema3A signaling / Eric F. Schmidt and Stephen M. Strittmatter -- GTPases in semaphorin signaling / Andreas W. Püchel -- Intracellular kinases in semaphorin signaling / Aminul Ahmed and Britta J. Eickholt -- Mical flavoprotein monooxygenases : structure, function and role in semaphorin signaling / Sharon M. Kolk and R. Jeroen Pasterkamp -- Signaling of secreted semaphorins in growth cone steering / Sangwoo Shim and Guo-li Ming -- Modulation of semaphorin signaling by IG superfamily cell adhesion molecules / Ahmad Bechara ... [et al.] -- Proteoglycans as modulators of axon guidance cue function / Joris de Wit and Joost Verhaagen -- Semaphorin signals in cell adhesion and cell migration : functional role and molecular mechanisms / Andrea Casazza, Pietro Fazzari, and Luca Tamagnone -- Semaphorin signaling during cardiac development / Toshihiko Toyofuku and Hitoshi Kikutani -- Semaphorin signaling in vascular and tumor biology / Gera Neufeld ... [et al.].
  • Sensing in nature 2012
    Also available: Print – 2012
    Thermosensor systems in eubacteria / Wolfgang Schumann -- Molecular plant volatile communication / Jarmo K. Holopainen and James D. Blande -- Primary processes in sensory cells : current advances / Stephan Frings -- Chemical communication in insects : the peripheral odor coding system of Drosophila melanogaster / Narelle E. Tunstall and Coral G. Warr -- Eusocial evolution and the recognition systems in social insects / Michelle O. Krasnec and Michael D. Breed -- Odor and pheromone sensing via chemoreceptors / Minghong Ma -- Identifying self- and nonself-generated signals : lessons from electrosensory systems / Angel Ariel Caputi and Javier Nogueira -- Magnetoreception / Roswitha Wiltschko and Wolfgang Wiltschko -- The molecular basis of mechanosensory transduction / Kara L. Marshall and Ellen A. Lumpkin -- The evolution of vertebrate color vision / Gerald H. Jacobs -- Transforming the vestibular system one molecule at a time : the molecular and developmental basis of vertebrate auditory evolution / Jeremy S. Duncan and Bernd Fritzsch -- Neurobiology of sociability / Heather K. Caldwell -- Changing senses : chemosensory signaling and primate evolution / Emily R. Liman -- Molecules and mating : positive selection and reproductive behavior in primates / Leslie A. Knapp and Simeon H.S. Innocent -- Immune systems evolution / Ramón M. Rodríguez, Antonio López Vázquez and Carlos López Larrea -- Involvement of sirtuins in life-span and aging related diseases / Ulrich Mahlknecht and Barbara Zschoernig -- Molecular diversity of Dscam and self-recognition / Lei Shi and Tzumin Lee -- The neural basis of semantic and episodic forms of self-knowledge : insights from functional neuroimaging / Arnaud D'Argembeau and Eric Salmon -- Hallmarks of consciousness / Ann B. Butler.
  • Ischemic tolerance in the brain : models and mechanisms / Michael Bell, Kelly Drew, Mark Smith and John Hallenbeck -- Regulation of gene expression by hypoxia in lung alveolar epithelial cells / Christine Clerici, Tokujiro Uchida, Carole Planès and Michael A. Matthay -- A profile of the metabolic responses to anoxia in marine invertebrates / Kevin Larade and Kenneth B. Storey -- The role of adenosine in tissue protection during ischemia-reperfusion / Gail W. Sullivan and Joel Linden -- NF-kB function in inflammation, cellular stress and disease / Neil R. Chapman, Sonia Rocha, Ian M. Adcock and Neil D. Perkins -- MAPping stress survival in yeasts : from the cell surface to the nucleus / Aaron Ngocky Nguyen and Kazuhiro Shiozaki -- Calcium signaling mediated by cyclic ADP-ribose and NAADP : roles in cellular response to stress / Hon Cheung Lee -- The cellular and molecular basis of the detection of pain / Jennifer K. Bonnington, David R. Robinson, Vittorio Vellani and Peter A. McNaughton -- Acquired freezing tolerance in higher plants : the sensing and molecular responses to low nonfreezing temperatures / Zhanguo Xin -- Sensing and responses to low temperature in cyanobacteria / Dmitry A. Los and Norio Murata -- Dehydrins / Jan Svensson, Abdelbagi M. Ismail, E. Tapio Palva and Timothy J. Close -- Dual role of membranes in heat stress : as thermosensors they modulate the expression of stress genes and, by interacting with stress proteins, re-organize their own lipid order and functionality / László Vígh and Bruno Maresca -- Cellular adaptation to amino acid availability: Mechanisms involved in the regulation of gene expression and protein metabolism / Sylvie Mordier, Alain Bruhat, Julien Averous and Pierre Fafournoux -- Amino acid-dependent signal transduction / Peter F. Dubbelhuis and Alfred J. Meijer -- Signal transduction pathways involved in the regulation of drug metabolizing enzymes / Vidya Hebbar and A.-N. Tony Kong -- Biological actions of infrared radiation / Lee Laurent-Applegate and Stéphanie Roques -- Energy sensing and photostasis in photoautotrophs / Norman P.A. Huner, Alexander G. Ivanov, Kenneth E. Wilson, Ewa Miskiewicz, Marianna Krol and Gunnar Öquist -- The uncoupling proteins family: From thermogenesis to the regulation of ROS / Marie-Clotilde Alves-Guerra, Claire Pecqueur, Alison Shaw, Elodi Couplan, Maria Del Mar, Gonzalez Barroso, Daniel Ricquier, Frédéric Bouillaud and Bruno Miroux -- Regulation of proliferation, differentiation and apoptosis of brown adipocytes : signal transduction pathways involved / Almudena Porras and Manuel Benito -- Control analysis of metabolic depression / R. Keira Curtis, Tammie Bishop and Martin D. Brand -- Evolution of physiological adaptation / Douglas L. Crawford -- Dynamic use of cDNA arrays : heterologous probing for gene discovery and exploration of organismal adaptation to environmental stress / Sean F. Eddy and Kenneth B. Storey.
  • Introduction, Clinical and Technical Aspects-- Normal Cytology-- Reactive Mesothelial Hyperplasia-- Malignant Mesothelioma-- A Potential Litigation Diagnosis-- Metastatic Cancers-- Effusions in Children-- Abdominopelvic Washings-- Lymphocytic Effusions-- Benign Inflammatory and Other Uncommon Conditions-- Cerebrospinal Fluid.
  • Sertoli cell biology 2005
    Also available: Print – 2005
  • Also available: Print – 2007
  • 2009 ClinicalKey
    "A reference on cellular-signalling processes, Signal Transduction second edition details the sources, key observations, and experiments that set the scene for recent and current scientific endeavour. Beginning with a historical overview, this book discusses how the concept of chemical messengers arose in the early twentieth century and shaped our current understanding of the action of hormones, cytokines, neurotransmitters, and growth factors." "The book further examines more complex signalling cascades, emanating from classic receptors, as well as adhesion molecules, involved in processes such as vision, smell, inflammation, innate and adaptive immunity, glucose homeostasis, cell-fated decisions, cell differentiation, and cell transformation. This revision concludes with a discourse on the targeting of signal-transduction cascades in the treatment of cancer and the structure of protein domains key to the assembly of signalling complexes"--Book jacket.
  • Machine generated contents note: 1 Regulation of SignalTransduction by the Fcy Receptor Family -- Members and Their Involvement in Autoimmunity -- Coggeshall, K.M. (Oklahoma City, Okla.) -- 30 The Role ofTNF/TNFR in Organ-Specific and Systemic -- Autoimmunity: Implications for the Design of Optimized 'Anti-TNF' -- Therapies -- Kollias, G.; Kontoyiannis, D.; Douni, E.; Kassiotis, G. (Vari) -- 51 CD40 Signaling and Autoimmunity -- Cheng, G. (Los Angeles, Calif.); Schoenberger, S.P. (San Diego, Calif.) -- 62 TGF-j: Receptors, Signaling Pathways and Autoimmunity -- Chen, W; Wahl, S.M. (Bethesda, Md.) -- 92 lnterleukin-2 Signaling and the Maintenance of Self-Tolerance -- Nelson, B.H. (Seattle, Wash.) -- 113 Role of the B7-CD28/CTLA-4 Pathway in Autoimmune Disease -- Chang, T.T.; Kuchroo, VK.; Sharpe, A.H. (Boston, Mass.) -- 131 AbnormalT Lymphocyte Signal Transduction in Systemic Lupus -- Erythematosus -- Kammer, G.M. (Winston-Salem, N.C.); Tsokos, G.C. -- (Bethesda, Md./Silver Spring, Md.) -- 151 Lyn/CD22/SHP-1 and Their Importance in Autoimmunity -- Blasioli, J.; Goodnow, C.C. (Canberra) -- 161 Regulation of Immune Responses by E3 Ubiquitin-Protein Ligases -- Fang, N.; Fang, D.; Wang, H.-Y.; Altman, A.; Liu, Y.-C. (San Diego, Calif.) -- 176 Keeping theT-Cell Immune Response in Balance: Role of Protein -- Tyrosine Phosphatases in Autoimmunity -- Mustelin, T. (La Jolla, Calif.) -- 191 Author Index -- 192 Subject Index.
  • Print Material
  • Noninvasive metabolic assessment of single cells / David K. Gardner -- Laser capture microdissection for analysis of single cells / Nobuki Nakamura ... [et al.] -- Fluorescence in situ hybridization (FISH) on single cells (sex determination and chromosome rearrangements) / Paul N. Scriven and Caroline Mackie Ogilvie -- Single cell PCR for PGD : methods, strategies, and limitations / Karen Sermon and Martine De Rycke -- Real-time quantitative PCR measurement of male fetal DNA in maternal plasma / Bernhard Zimmermann ... [et al.] -- Cell-free fetal DNA plasma extraction and real-time PCR quantification / Jill L. Maron, Kirby L. Johnson, and Diana W. Bianchi -- LATE-PCR and allied technologies : real-time detection strategies for rapid, reliable diagnosis from single cells / Kenneth E. Pierce and Lawrence J. Wangh -- Efficient isothermal amplification of the entire genome from single cells / Karen V. Schowalter, Jolene R. Fredrickson, and Alan R Thornhill -- Comparative genomic hybridization on single cells / Lucille Voullaire and Leeanda Wilton -- Generation of amplified RNAs and cDNA libraries from single mammalian cells / James Adjaye -- Real-time PCR to measure gene expression in single cells / Dagan Wells -- Gender determination and detection of aneuploidy in single cells using DNA array-based comparative genomic hybridization (array CGH) / Dong Gui Hui, Xin Yuan Guan, and Nicole Hussey -- Microarray technology for mutation analysis of low template DNA samples / Chelsea Salvado and David Cram.
  • Also available: Print – 2013
  • Sorting and Recycling Endosomes provides the latest information on endosomes, the receiving compartment for endocytosed cargos, and the donor compartment and sorting station for cargos designated to lysosomes, Golgi, or plasma membrane. In recent years, the importance of endosomes as a sorting and recycling compartment has become increasingly appreciated. As such, scientists from various fields of cell biology, membrane traffic, and beyond, see the needs to communicate and learn about the methods used to investigate the dynamics and functions of endosomes. This book brings together specialists from the field who contribute their expertise on a broad range of biomedical topics that will provide ideal reading for researchers interested in endosomal sorting and recycling. This volume covers the approaches necessary to study the key components that mediate the generation and transport of membrane-bounded carriers from the endosomes, and how membrane trafficking machinery is coordinated with cytoskeletons during these processes. In addition to studies carried out in mammalian cells, other model systems such as worm and yeast are also included.
  • This new volume on gene expression and epigenetics discusses environmental effects related to specific gene expression. The book also shows methods for bioinformatic analysis of the epigenome. The book is broken into two sections: the first looks at eukaryotic DNA methylation and the second addresses how to integrate genomic medicine into clinical practice. The book includes chapters on these topics: Gene expression in colon cancer tissue Epigenetics in human acute kidney injury Embryologically relevant candidate genes in MRKH patients DNA methylation in common skeletal disorders Causal relat.
  • Sphingolipids are lipid components of the plasma membrane in eukaryotic cells. They have an important function in signaling mechanisms in the cell. This book on sphingolipids provides insights into the basics of sphingolipid biology and drug development, with a particular emphasis on the sphingolipid derivative ceramide. In the first part basic functions of sphingolipids are described, as well as the genetics of important enzymes, sphingolipid metabolism and synthesis. The second part of this first volume focuses on drug development and pharmacology. The book is intended for scientists in pharmacology, biochemistry and cell biology with a focus on biomedical research as well as for clinicians working in pharmacology, oncology, cardiology, neurology and infectious disease. Together with Volume 216 by the same editors, the collection represents a unique, comprehensive work on sphingolipids, providing information on both sphingolipids' basic biology (including synthesis, metabolism and cell biology) and their important function in a (patho-)physiological context.
  • Heart -- Neuro -- Musculoskeletal -- Kidney -- Eye -- Pancreas -- Hematology -- Skin.
  • Stem cell assays 2007
    Springer Protocols
    Derivation of human embryonic stem cells in xeno-free conditions / Mohan C. Vemuri ... [et al.] -- Feeder-layer free culture system for human embryonic stem cells / Michal Amit -- Digital imaging of stem cells by electron microscopy / A. Henry Sathananthan and Stefania A. Nottola -- A controlled-cooling protocol for cryopreservation of human and non-human primate embryonic stem cells / Carol B. Ware and Szczepan W. Baran -- Cell surface markers in human embryonic stem cells / Raj R. Rao, Alison Venable Johnson, and Steven L. Stice -- Generation of a monoclonal antibody library against human embryonic stem cells / Micha Drukker, Christina Muscat, and Irving L. Weissman -- Analytical methods for cancer stem cells / Vinagolu K. Rajasekhar -- Micro RNA profiling : an easy and rapid method to screen and characterize stem cell populations / Uma Lakshmipathy ... [et al.] -- Gene transfer via nucleofection into adult and embryonic stem cells / Uma Lakshmipathy, Shannon Buckley, and Catherine Verfaillie -- RNAi knockdown of transcription factor Pu.1 in the differentiation of mouse embryonic stem cells / Gang-Ming Zou, Meredith A. Thompson, and Mervin C. Yoder -- StemBase : a resource for the analysis of stem cell gene expression data / Christopher J. Porter ... [et al.] -- Isolation of stem cells from human umbilical cord blood / Nishanth P. Reddy, Mohan C. Vemuri, and Reddanna Pallu -- Ex vivo expansion of hematopoietic stem cells from human cord blood in serum-free conditions / Chao-Ling Yao and Shiaw-Min Hwang -- Hematopoietic colony-forming cell assays / Carla Pereira, Emer Clarke, and Jackie Damen -- Assays for alloreactive responses by PCR / Patrick Stordeur -- Immune properties of mesenchymal stem cells / Panagiota A. Sotiropoulou and Michael Papamichail -- Clinical grade expansion of human bone marrow mesenchymal stem cells / Panagiota A. Sotiropoulou, Sonia A. Perez, and Michael Papamichail -- Adenoviral transduction of mesenchymal stem cells / Pablo Bosch and Steven L. Stice -- Directed differentiation of human embryonic stem cells to dendritic cells / Maxim A. Vodyanik and Igor I. Sluvkin -- Insulin-producing cells from embryonic stem cells to dendritic cells / Maxim A. Vodyanik and Igor I. Slukvin -- Insulin-producing cells from embryonic stem cells experimental considerations / Enrique Roche ... [et al.] -- Efficient generation of dopamine neurons from human embryonic stem cells / Chang-Hwan Park and Sang-Hun Lee -- Isolation of oligodendroglial cells from cultured neural stem/progenitors / Frank Zeigler and Stephen G. Hall -- Differentiation of human embryonic stem cells toward the chondrogenic lineage / Wei Seong Toh ... [et al.] -- Cartilage tissue engineering : directed differentiation of embryonic stem cells in three-dimensional hydrogel culture / Nathaniel S. Hwang, Shyni Varghese, and Jennifer Elisseeff.
  • Patient specific and disease specific stem cell lines have already introduced groundbreaking advances into the research and practice of ophthalmology. This volume provides a comprehensive and engaging overview of the latest innovations in the field. Twelve chapters discuss the fastest growing areas in ophthalmological stem cell research, from disease modeling, drug screening and gene targeting to clinical genetics and regenerative treatments. Innovative results from stem cell research of the past decade are pointing the way toward practicable treatments for retinitis pigmentosa, age related macular degeneration, and Stargardt disease. What future directions will stem cell research take? Researchers, graduate students, and fellows alike will find food for thought in this insightful guide tapping into the collective knowledge of leaders in the field. Stem Cells in Ophthalmology is part of the Stem Cells in Regenerative Medicine series dedicated to discussing current challenges and future directions in stem cell research.
  • Part 1. Bone marrow-derived stem cells -- Part 2. Embryonic stem cells -- Part 3. Cancer stem cells.
  • Stem cell culture 2008
    Also available: Print – 2008
  • "Stem Cell Labeling for Delivery and Tracking Using Noninvasive Imaging provides a comprehensive overview of cell therapy imaging, ranging from the basic biology of cell therapeutic choices to the preclinical and clinical applications of cell therapy. It emphasizes the use of medical imaging for therapeutic delivery/targeting, cell tracking, and determining therapeutic efficacy. The book first presents background information and insight on the major classes of stem and progenitor cells. It then describes the main imaging modalities and state-of-the-art techniques that are currently employed for stem cell tracking. In the final chapters, leading scholars offer clinical perspectives on existing and potential uses of stem cells as well as the impact of image-guided delivery and tracking in major organ systems. Through clear descriptions and color images, this volume illustrates how noninvasive imaging is used to track stem cells as they repair damaged tissue in the body. With contributions from some of the most prominent preclinical and clinical researchers in the field, the book helps readers to understand the evolving concepts of stem cell labeling and tracking as the field continues to move forward"--Provided by publisher.
  • Immunostaining of germline stem cells and the niche in Drosophila ovaries / Lichao Luo, Phing Chian Chai, and Yu Cai -- Genetic, immunofluorescence labeling, and in situ hybridization techniques in identification of stem cells in male and female germline niches / Shree Ram Singh ... [et al.] -- Visualization of adult stem cells within their niches using the Drosophila germline as a model system / Annekatrin König and Halyna R. Shcherbata -- Morphometric evaluation of the spermatogonial stem cell distribution and niche in vertebrates / Paulo Henrique Almeida Campos-Junior ... [et al.] -- In vitro construction of 2D and 3D simulations of the murine hematopoietic niche / Brahmananda Reddy Chitteti ... [et al.] -- Isolation of embryonic hematopoietic niche cells by flow cytometry and laser capture microdissection / Daisuke Sugiyama and Tatsuya Sasaki -- Isolation and enrichment of Stro-1 immunoselected mesenchymal stem cells from adult human bone marrow / Emma L. Williams, Kate White, and Richard O.C. Oreffo -- Primary marrow-derived stromal cells : isolation and manipulation / Aravind Ramakrishnan, Beverly Torok-Storb, and Manoj M. Pillai -- Detection in vitro and quantitative estimation of artificial microterritories which promote osteogenic differentiation and maturation of stromal stem cells / Igor A. Khlusov, Natalya M. Shevtsova, and Marina Yu. Khlusova -- Prospective isolation of viable, high ploidy megakaryocytes from adult murine bone marrow by fluorescence activated cell sorting / Shen Y. Heazlewood ... [et al.] -- Looking for the niche : substance delivery into the lateral ventricle of the brain : the osmotic minipump system / María Victoria Gómez Gaviro ... [et al.] -- Unbiased stereological method to assess proliferation throughout the subependymal zone / Ana Mendanha Falcão ... [et al.] -- Cardiac stem cell niche, MMP9, and culture and differentiation of embryonic stem cells / Paras Kumar Mishra ... [et al.] -- Human and murine skeletal muscle reserve cells / Rana Abou-Khalil, Fabien Le Grand, and Bénédicte Chazaud -- Modulation of the host skeletal muscle niche for donor satellite cell grafting / Luisa Boldrin and Jennifer E. Morgan -- Isolation of c-Kit+ human amniotic fluid stem cells from second trimester / Michela Pozzobon ... [et al.] -- Hypoxia and visualization of the stem cell niche / Ali Dalloul -- Detection and isolation of human disseminated tumor cells in the murine bone marrow stem cell niche / Yusuke Shiozawa, Russell S. Taichman, and Evan T. Keller -- Identification and separation of normal hematopoietic stem cells and leukemia stem cells from patients with acute myeloid leukemia / Van T. Hoang ... [et al.] -- Serial orthotopic transplantation of epithelial tumors in single-cell suspension / Heather A. McCauley and Géraldine Guasch -- Isolation and propagation of colon cancer stem cells / Pramudita R. Prasetyanti ... [et al.].
  • Ventricular remodeling in ischemic cardiomyopathy / Stefan Klotz and Daniel Burkhoff -- Myocardial regeneration: which cell and why / Elmostafa El Fahime and Jacques Tremblay -- Cardiac stem cells for myocardial regeneration / Bernardo Nadal-Ginard and Simón Méndez-Ferrer -- A historic recapitulation of myoblast transplantation / Daniel Skuk and Jacques Tremblay -- Myoblast cell transplantation preclinical studies / Doris A. Taylor and Harald Ott -- Skeletal myoblasts: the European experience / Philippe Menasche -- Skeletal myoblasts: the U. S. experience / Edward B. Diethrich -- Progenitor cells for cardiac regeneration / Ana Sánchez and Javier Garcia-Sancho -- Bone marrow derived stem cell for myocardial regeneration: preclinical experience / Bradley Martin and Mark Pittenger -- Bone marrow derived stem cell for myocardial regeneration: clinical experience, surgical delivery / Manual Galiñanes -- Autologous mononuclear bone marrow cell transplantation for myocardial infarction: the German experience / Michael Brehm, Tobias Zeus and Bodo E. Strauer -- Autologous mononuclear bone marrow cell transplantation for myocardial infarction: the Spanish experience / Francisco E. Avilés ... [et al.] -- Mobilizing bone marrow stem cells for myocardial repair after acute myocardial infarction / Steve Ellis and Oussama Wazni -- Percutaneous myoblast transplantation: steps in transplantation research / Nabil Dib -- A porcine model of myocardial infarction for evaluation of cell transplantation / Nabil Dib ... [et al.] -- Tissue engineering for myocardial regeneration / Ravi K. Birla -- The role of pet scan in stem cell therapy / Uchechukwa Sampson ... [et al.] -- The measurement of systolic function in the mammalian heart / Blasé Carabello -- Electrophysiological aspects of cell transplantation / Nicholas S. Peters, Nicolas A.F. Chronos and Fernando Tondato -- Regulatory considerations in manufacturing, product testing, and preclinical development of cellular products for cardiac repair / Ellen Areman, Kim Benton and Richard McFarland.
  • 1. Lysosomal diseases and therapeutic options: an overview / Eveline J. Langereis and Frits A. Wijburg -- 2. Alternative treatment options: enzyme replacement and small molecule therapies / James Edmond Wraith and Simon Jones -- 3. Hematopoietic cell transplantation in inborn errors of metabolism / Jaap J. Boelens, Paul Szaboles, and Rob Wynn -- 4. Leukodystrophies and lysosomal storage disorders / Maria L. Escolar and Mieke Aldenhoven -- 5. Hematopoietic stem cell gene therapy for lysosomal storage disorders: expected benefits and limitations / Alessandra Biffi and Ilaria Visigalli -- 6. Alternative future therapies for lysosomal storage diseases: embryonic stem cell- and induced pluripotent stem cell therapy / Arie Koen Braat, Paul J. Coffer, and Niels Geijsen -- 7. Concluding comments and future directions / Robert Wynn and Jaap Jan Boelens.
  • Also available: Print – 2006
    In vitro experimentation and research tools -- Tissue engineering and regenerative medicine.
  • The discovery and isolation of human embryonic stem cells (hESCs), and the more recent generation of induced pluripotent stem cells (iPSCs) from adult cells, has given medical science the tantalizing prospect of one day regenerating organs and tissues in human patients, as well as a revolutionary method for investigating heritable human diseases in a petri dish. This is because hESCs and iPSCs are pluripotent, which enables them to differentiate into virtually any cell type of the human body. However, forcing these cells to change their phenotype is an imperfect science, and is often time-consuming, resource-intensive, and plagued by poor yields. My work over the past four years has therefore attempted to characterize the sets of molecules, both messenger RNAs and microRNAs, that together regulate pluripotency. This has included understanding how the stem cell "transcriptome" changes in the face of external insult (e.g. ionizing radiation), how it changes during differentiation to adult phenotypes such as cardiomyocytes and endothelial cells, and how knowledge from it may be used to induce pluripotency. Taken together, interrogating and ultimately controlling the stem cell transcriptome will be an essential step before we can realize the promise of regenerative therapy.
  • Also available: Print – 2012
    Section I. Basic Aspects of Transplantation -- The Frontiers of Organ Transplantation and Cell Therapy / Rafael Matesanz -- Organ Transplantation in the 21th Century / Francisco Ortega -- Immunology and the Challenge of Transplantation / Rebeca Alonso Arias, Antonio López-Vázquez and Carlos López-Larrea -- Cellular Immunotolerance in the Transplant / Marcos Lóez-Hoyos, David San Segundo and Manuel Arias -- Immunosuppression in the ERA of Biological Agents / Josep M. Grinyó, Josep M. Cruzado, Oriol Bestard, J. R. Vidal Castiñeira and Joan Torras -- Transgenic Organs and Xenotransplants / Cristina Costa Vallés and Rafael Máñez Mendiluce -- Cell and Tissue Therapy in Regenerative Medicine / Ana Sánchez, Thomas Schimmang and Javier García-Sancho -- Section II. Adult Stem Cells -- Characteristics of Adult Stem Cells / Manuel A. Gonzalez and Antonio Bernad -- Bone Marrow Transplantation Extends Its Scope / Fermín M. Sánchez-Guijo, Alberto Orfao and María Consuelo del Cañizo -- Biology of Stem Cells: The Role of Microenvironments / Agustín G. Zapata, David Alfaro and Javier García-Ceca -- Mobilization and Homing of Hematopoietic Stem Cells / Beatriz Suárez-Álvarez, Antonio López-Vázquez and Carlos López-Larrea -- Great Promise of Tissue-Resident Adult Stem/Progenitor Cells in Transplantation and Cancer Therapies / Murielle Mimeault and Surinder K. Batra -- Multipotent Mesenchymal Stromal Cells: Clinical Applications and Cancer Modeling / René Rodríguez, Javier García-Castro, Cesar Trigueros, Mariano García Arranz and Pablo Menéndez -- Section III. Embryonic Stem Cells -- Neural Stem Cells and Transplantation Studies in Parkinson's Disease / Ricardo Pardal and José López-Barneo -- Biological Impact of Human Embryonic Stem Cells / Miguel Martín and Pablo Menéndez -- Epigenetics of Embryonic Stem Cells / Vincenzo Calvanese and Mario F. Fraga -- New Tools in Regenerative Medicine: Gene Therapy / Miguel Muñoz Ruiz and José R. Regueiro -- Therapeutic Cloning and Cellular Reprogramming / Ramon M. Rodriguez, Pablo J. Ross and Jose B. Cibelli -- Section IV. Stem Cell Regenerative Medicine -- Advances in Stem Cell Therapy / Silvia Pérez López and Jesús Otero Hernández -- Tissue Bioengineering and Artificial Organs / Sara Llames, Eva García, Jesús Otero Hernández and Álvaro Meana.
  • Chapter 1. Promises and Limitations in the Application of Cell Therapy for Tissue Regeneration / Raphael Gorodetsky -- Chapter 2. Adult Non-hematopoietic Stem Cells and Progenitor Cells (aNHSCs) / R. Schäfer and L. Dahéron -- Chapter 3. Screening Approaches for Stem Cells / David G. Buschke, Derek J. Hei, Kevin W. Eliceiri and Brenda M. Ogle -- Chapter 4. Hematopoietic Stem Cells and their Role in Regenerative Medicine / Eitan Fibach -- Chapter 5. Cord and Cord Blood: Valuable Resources with Potential for Liver Therapy / Saba Habibollah, Marcin Jurga, Nico Forraz and Colin McGuckin -- Chapter 6. Induced Pluripotent Stem Cells: Their Role in Modeling Disease and Regenerative Medicine / Yonatan Stelzer and Marjorie Pick -- Chapter 7. Mesenchymal Stromal/Stem Cells from Tissue Repair to Destruction of Tumor Cells / Rita Bussolari, Giulia Grisendi, Luigi Cafarelli, Pietro Loschi, Laura Scarabelli, Antonio Frassoldati, Michela Maur, Giorgio De Santis, Paolo Paolucci, Pierfranco Conte and Massimo Dominici -- Chapter 8. Fibrin-based Matrices to Support Stem Cell-Based Tissue Regeneration / Raphael Gorodetsky, Iris Mironi-Harpaz and Dror Seliktar -- Chapter 9. Culturing Non-hematopoietic Mesenchymal Stromal Cells and Requirements of GMP in Stem Cell-based Therapies / Karen Bieback, Marianna Karagianni, Gerlinde Schmidtke-Schrezenmeier, Natalie Fekete and Hubert Schrezenmeier -- Chapter 10. (Stem) Cell Based Therapy for Neurological Disorders / O. Einstein and T. Ben-Hur -- Chapter 11. Mesenchymal Osteogenic Precursors for Bone Repair and Regeneration / Nicola Baldini, Dante Dallari and Francesca Perut -- Chapter 12. Stem Cells and Cartilage Repair / Bernd Rolauffs, Andreas Badke, Kuno Weise, Alan J. Grodzinsky and Wilhelm K. Aicher -- Chapter 13. Stem Cell-based Replacement Tissue for Heart Repair / Ayelet Lesman and Shulamit Levenberg -- Chapter 14. Regeneration of the Vascular System / M. Schleicher, A. J. Huber, H. P. Wendel and U. A. Stock -- Chapter 15. Regenerative Strategies for the Endocrine Pancreas: From Islets to Stem Cells and Tissue Reprogramming / Juan Domínguez-Bendala and Camillo Ricordi -- Chapter 16. Regeneration of the Lower Urinary Tract: Clinical Applications and Future Outlook / Markus Renninger, Bastian Amend, Jörg Seibold, Gerhard Feil, Arnulf Stenzl and Karl-Dietrich Sievert -- Chapter 17. Effective Tissue Repair and Immunomodulation by Mesenchymal Stem Cells within a Milieu of Cytokines / Philip Lim, Shyam A. Patel and Pranela Rameshwar -- Chapter 18. Homing of Mesenchymal Stromal Cells / Reinhard Henschler, Erika Deak and Richard Schäfer.
  • Stem cells 2006
  • Also available: Print – 2002
  • pt. 1. Stem cell biology -- pt. 2. Epigenetic and microRNA regulation in stem cells -- pt. 3. Stem cells for therapeutic applications -- pt. 4. Nuclear reprogramming and induced pluripotent stem cells -- pt. 5. Tissue engineering -- pt. 6. Regenerative medicine.
  • Biology of the cell -- Embryonic development -- What are stem cells? -- Of mice and men: the history of the stem cell -- Origins and types of stem cells -- Cloning -- Regenerative medicine: clinical applications of stem cells -- Cardiomyocytes from stem cells: what can we do with them? -- Stem cell tourism --Stem cells in cancer and cancer stem cells -- Stem cells for discovery of effective and safe new drugs -- Legal and intellectual property issues associated with stem cells -- Stem cell perspectives: a vision of the future.
  • Use of Human Embryonic Stem Cells in Therapy -- Human Neural Stem Cell-based Cell- and Gene-therapy for Neurological Diseases -- Vascular Stem Cell Therapy -- Bioprocessing of Human Pluripotent Stem Cells for Cell Therapy Applications -- Blood Cell Bioprocessing: The Haematopoietic System and Current Status of In-vitro Production of Red Blood Cells -- Bioprocessing Challenges Associated with the Purification of Cellular Therapies -- Separation Technologies for Stem Cell Bioprocessing.
  • The main objective of this book is to provide a comprehensive review on stem cells and their role in tissue regeneration, homeostasis and therapy. In addition, the role of cancer stem cells in cancer initiation, progression and drug resistance are discussed. The cell signaling pathways and microRNA regulating stem cell self-renewal, tissue homeostasis and drug resistance are also mentioned. Overall, these reviews will provide a new understanding of the influence of stem cells in tissue regeneration, disease regulation, therapy and drug resistance in several human diseases.
  • Also available: Print – 2007
    The challenge for stem cell therapy: overview of the problem: heart attack and heart failure -- Hematopoietic stem cells for myocardial regeneration -- Mesenchymal stem cells for cardiac therapy -- Multipotent adult progenitor cells -- Mesenchymal progenitor cells for vascular formation and cardiac muscle regeneration -- Umbilical cord blood stem cells for myocardial regeneration and angiogenesis -- Endogenous cardiac stem cells -- Embryonic stem cells for myocardial repair -- Chemokin and homing factor expression in acute myocardial infarction and chronic heart failure -- Stem cell differentiation toward a cardiac myocyte phenotype -- Electrical coupling and/or ventricular tachycardia risk of cell therapy -- Cell therapy for myocardial damage: arrhythmia risk and mechanisms -- Aspects of percutaneous cellular cardiomyoplasty -- Stem cells and myocardial regeneration: open-chest/minimally invasive surgical techniques -- Measures of effective cell based therapies -- Whole bone marrow transplantation -- Clinical angioblast therapy -- Use of skeletal myoblasts for the treatment of chronic heart failure -- Bone marrow and angioblast transplantation -- Strategies for cytokine modification and stem cell mobilization for acute myocardial infarction -- 21 summary and future challenges.
  • Myogenic potential of stem cells: in vivo assessment / Maximilien Bencze [and five others] -- Urine-derived stem cells: biological characterization and potential clinical applications / Guihua Liu, Chunhua Deng and Yuanyuan Zhang -- Immunoregulatory properties of mesenchymal stem cells: in vitro and in vivo / Pinar Çetinalp Demircan [and three others] -- Current research on stem cells in Parkinson's Disease: progress and challenges / Ria Thomas and Jan Pruszak -- Stem cell applications in retinal diseases / Güngör Sobacı -- Potential use of dental stem cells for craniofacial tissue regeneration / Mustafa Ramazanoglu [and three others] -- Pediatric diseases and stem cells: recent advances and challenges / Duygu Uçkan -- Stem cell therapy: from the heart to the periphery / Bernd Stratmann and Diethelm Tschoepe -- Stem cells in wound healing / Dilek Telci, Ayca Zeynep Ilter and Merve Erdem -- Cancer stem cells: the gist of the matter / Torben Redmer [and five others] -- Filling the gap in the relationship between cancer and stem cells / Erdal Karaoz [and two others] -- Neural stem cells as therapeutic delivery vehicles for malignant brain tumors / Tugba Bagci-Onder -- Therapy resistance in prostate cancer: a stem cell perspective / Sandra Klein [and three others] -- Mechanisms of somatic cell reprogramming / Tamer T. Onder -- Disease modeling and drug discovery using human pluripotent stem cells / Christian Honoré and Mattias Hansson -- Stem cell research: ethical considerations / Berna Arda [and two others].
  • Stem cells for myocardial repair and regeneration : where are we today? / Randall J. Lee -- Bone marrow-derived endothelial progenitor cells : isolation and characterization for myocardial repair / Masaaki Ii -- Umbilical cells stem cells for myocardial repair and regeneration / Nicholas Greco and Mary J. Laughlin -- Isolation of resident cardiac progenitor cells by Hoechst 33342 staining / Otmar Pfister ... [et al.] -- Mesenchymal stem cell therapy for cardiac repair / Andrew J. Boyle, Ian K. McNiece, and Joshua M. Hare -- Methods for human embryonic stem cells derived cardiomyocytes cultivation, genetic manipulation, and transplantation / Gil Arbel ... [et al.] -- Experimental cell transplantation therapy in rat myocardial infarction model including nude rat preparation / Wangde Dai and Robert A. Kloner -- Large animal model of heart failure for assessment of stem cells / Sharad Rastogi -- Histopathologic assessment of myocardial regeneration / Naima Carter-Monroe ... [et al.] -- Assessment of myocardial angiogenesis and vascularity in small animal models / Matthew L. Springer -- Superparamagnetic iron oxide labeling of stem cells for MRi tracking and delivery in cardiovascular disease / Dorota A. Kedziorek and Dara L. Kraitchman -- Embryonic stem cell biology : insights from molecular imaging / Karim Sallam and Joseph C. Wu -- Genetic fate-mapping for studying adult cardiomyocyte replenishment after myocardial injury / Sunny S.-K. Chan ... [et al.] -- In vitro electrophysiological mapping of stem cells / Seth Weinberg, Elizabeth A. Lipke, and Leslie Tung -- Assessment of cardiac conduction : basic principles of optical mapping / Chunhua Ding and Thomas H. Everett IV -- Surface patterning for generating defined nanoscale matrices / Karen L. Christman and Heather D. Maynard.
  • Interest in the use of stem cells in aesthetic procedures has been increasing rapidly, reflecting the widespread acknowledgment of the tremendous potential of stem cell fat transfer. This is, however, the first book to be devoted entirely to the subject. The book opens by reviewing the history of the development of pluripotent stem cells and the results of research into the biochemistry and physiology of stem cells. Adipose tissue anatomy and survival are discussed, and the wide range of aesthetic procedures involving stem cell fat transfer are then described in detail. These procedures relate to the face, breast, buttocks, legs, hands, penis, and Poland syndrome. In addition, potential risks and complications are identified. Stem Cells in Aesthetic Procedures: Art, Science, and Clinical Techniques is completely up to date and has been written by leading experts in the field. It will be an invaluable source of information for students, beginners, and experienced surgeons in the fields of plastic surgery, general surgery, cosmetic surgery, facial plastic surgery, otolaryngology, ophthalmology, and oral maxillofacial surgery.
  • Derivation and manipulation of murine embryonic stem cells / Alexander Meissner, Sarah Eminli, and Rudolf Jaenisch -- Patterning mouse and human embryonic stem cells using micro-contact printing / Raheem Peerani ... [et al.] -- Efficient gene knockdowns in human embryonic stem cells using lentiviral-based RNAi / Asmin Tulpule and George Q. Daley -- Measurement of cell-penetrating peptide-mediated transduction of adult hematopoietic stem cells / Aziza P. Manceur and Julie Audet -- Stem cell sources for regenerative medicine / Ali M. Riazi, Sarah Y. Kwon, and William L. Stanford -- Investigating the interactions between haemopoietic stem cells and their niche : methods for the analysis of stem cell homing and distribution within the marrow following transplantation / Brenda Williams and Susan K. Nilsson -- Ex vivo megakaryocyte expansion and platelet production from human cord blood stem cells / Valérie Cortin, Nicolas Pineault, and Alain Garnier -- Ex vivo generation of human red blood cells : a new advance in stem cell engineering / Luc Douay and Marie-Catherine Giarratana -- Isolation and manipulation of mammalian neural stem cells in vitro / Claudio Giachino, Onur Basak, and Verdon Taylor -- Isolation, expansion, and differentiation of mouse skin-derived precursors / Karl J.L. Fernandes and Freda D. Miller -- Xenotransplantation of embryonic stem cell-derived motor neurons into the developing chick spinal cord / Hynek Wichterle, Mirza Peljto, and Stephane Nedelec -- Transplantation of neural stem/progenitor cells into developing and adult CNS / Tong Zheng ... [et al.] -- Transplantation of embrynic stem cell-derived dopaminergic neurons in MPTP-treated monkeys / Jun Takahashi, Yasushi Takagi, and Hidemoto Saiki -- Isolation and culture of epithelial stem cells / Jonathan A. Nowak and Elaine Fuchs -- Regeneration of skin and cornea by tissue engineering / Danielle Larouche ... [et al.] -- Prospective isolation of mesenchymal stem cells from mouse compact bone / Brenton J. Short, Nathalie Brouard, and Paul J. Simmons -- Isolation, propagation, and characterization of human umbilical cord perivascular cells (HUCPVCs) / Rahul Sarugaser ... [et al.] -- Bone marrow-derived mesenchymal stem cells : isolation, expansion, characterization, viral transduction, and production of conditioned medium / Massimilano Gnecchi and Luis G. Melo -- Template DNA-strand co-segregation and asymmetric cell division in skeletal muscle stem cells / Vasily Shinin, Barbara Gayraud-Morel, and Shahragim Tajbakhsh -- Isolation and grafting of single muscle fibres / Charlotte A. Collins and Peter S. Zammit -- Differentiation and dynamic analysis of primitive vessels from embryonic stem cells / Gefei Zeng and Victoria L. Bautch -- Derivation of contractile smooth muscle cells from embryonic stem cells / Sanjay Sinha, Mark H. Hoofnagle, and Gary K. Owens -- Islet-derived progenitors as a source of in vitro islet regeneration / Stephen Hanley and Lawrence Rosenberg -- Isolation and characterization of hepatic stem cells, or "oval cells," from rat livers / Thomas D. Shupe ... [et al.] -- Reprogramming of liver to pancreas / Shifaan Thowfeequ ... [et al.]
  • "In this state-of-the-art exploration of a hugely dynamic and fast-evolving field of research, leading researchers share their collective wisdom on the role that stem cells could play in the context of physiological stress and lung injury. The text focuses on reviewing the most relevant -- and recent -- ideas on using local, endogenous, and exogenous progenitor/stem cells in preventing and treating injury to the lung. The lungs are one of the most complex organs in the human body, with a mature adult lung boasting at least 40 morphologically differentiated cell lineages. Our entire blood supply passes through the lung's alveolar units during oxygenation. This interaction with the outside world, along with the intricacies of its structure, makes the lung a highly susceptible organ that is vulnerable to numerous types of injury and infection. This means that the mechanisms of lung repair are in themselves correspondingly complex. Because of their multipotentiality, as well as the fact of the lung's relatively rapid cell turnover, stem cells are thought to be an important alternative cell-base therapy in lung injury. Despite the controversial nature of stem cell research, there has been growing interest in both local and endogenous stem cells in the lung. This highly topical book with chapters on everything from using mesenchymal stem cells in lung repair to the effect of physical activity on the mobilization of stem and progenitor cells, represents an exciting body of work by outstanding investigators and will be required reading for those with an interest in the subject."--Publisher's website.
  • Fulltext v. 46 (2008) ScienceDirect
  • v. 1A From cells to vesicles -- v. 1B Generic and specific interactions. Biological membranes architecture and function / E. Sackmann -- The evolution of membranes / M. Bloom and O.G. Mourttsen -- Polymorphism of lipid-water systems / J.M. Seddon and R.H. Templer -- Phospholipid monolayers / H. Möhwald -- Physical basis of self-organization and function of membranes : physics of vesicles / E. Sackmann -- Lateral diffusion in membranes / P.F.F. Almeida and W.L.C. Vaz -- Molecular theory of chain packing, elasticity and lipid-protein interaction in lipid bilayers / A. Ben-Shaul -- Morphology of vesicles / U. Seifert and R. Lepowsky -- Material transport across permeability barriers by means of lipid vesicles / G. Cevc -- Applications of liposomes / D.D. Lasic -- Generic interactions of flexible membranes / R. Lipowsky -- Electrostatic properties of membranes : the Poisson-Boltzmann theory / D. Andelman -- Interaction in membrane assemblies / V.A. Parsegian and R.P. Rand -- Tension-induced mutual adhesion and a conjectured superstructure of lipid membranes / W. Helfrich -- Physical actions in biological adhesion / E. Evans -- Adhesion of cells / P. Bongrand -- Cell membranes and the cytoskeleton / P. Janmey -- Electroporation and electrofusion of membranes / D.S. Dimitrov -- Cation-induced vesicle fusion modulated by polymers and proteins / K. Arnold.
  • Also available: Print – 2010
    Part I. Structure and ligands of NCAM. -- Structural biology of NCAM / Vladislav Soroka, Christina Kasper, Flemming M. Poulsen. -- Extracellular protein interactions mediated by the neural cell adhesion molecule, NCAM: heterophilic interactions between NCAM and cell adhesion molecules, extracellular matrix proteins, and viruses / Janne Nielsen, Nikolaj Kulahin, Peter S. Walmod. -- Intracellular (intracelluar) ligands of NCAM / Bettina Buttner, Rudiger horstkorte. -- Role of ATP in the regulation of NCAM function / Martin V. Hubschmann, Galina Skladchikova. -- Part II. NCAM and plysialic acid. -- Polysialylation of NCAM / Herbert Hildebrandt, Martina Muhlenhoff, Rita Gerardy-Schahn. -- Structural basis for the polysialylation of the neural cell adhesion molecule / Karen J. Colley. -- Role of PSA-NCAM in adult neurogenesis / Eduardo Gascon, Laszlo Vutskits, Jozsel Zoltan Kiss. -- Use of PSA-NCAM in repair of the central nervous system / Abderrahman El Maarouf, Urs Rutishauser. -- Part III. NCAM-mediated signal transduction. -- Signaling pathways involved in NCAM-induced neurite outgrowth / Dorte Kornerup Ditlevsen, Kateryna Kolkova. -- Role of the growth-associated protein GAP-43 in NCAM mediated neurite outgrowth / Irina Korshunova, Mark Mosevitski. -- Neural cell adhesion molecule NCAM and lipid rafts / Gro Klitgaard Povlsen, Dorte Kornerup Ditlevsen. -- Neural cell adhesion molecule and epidermal growth factor receptor: signaling crosstalk / Gro Klitgaard Polvsen. -- Part IV. NCAM metabolism. -- Biosynthesis of NCAM / Kateryna Kolkova. -- Soluble NCAM / Thomas Secher. -- Part V. NCAM, synapses, emotions, and memory. -- Role of NCAM in spine dynamics and synaptogenesis / D. Muller, P. Mendez, M. DeRoo, P. Klauser, S. Steen, and L. Poglia. -- NCAM in long-term potentiation and learning / Barbara P. Hartz, Lars Christian B. Roon. -- Role of NCAM in emotion and learning / Lisa Conboy, Reto Bisaz, Kamila Markram, Carment Sandi. -- Part VI. NCAM in disease. -- NCAM in neuropsychiatric and neurodegenerative disorders / Leann H. Brennaman, Patricia F. Maness. -- Neural cell adhesion molecule in cancer: expression and mechanisms / Silvia Zecchini, Ugo Caallaro. -- Part VII. Pharmacology of NCAM: NCAM mimetics. -- NCAM mimetic peptides: an update / Vladimir Berezin, Elisabeth Bock. -- Synthetic NCAM-derived ligands of the fibroblast growth factor receptor / Stine maria Hansen, Shizhong Li, Elisabeth Bock, Vladimir Berezin. -- Dendritic spine and synapse morphological alterations induced by neural cell adhesion molecule mimetic / Michael Stewart, Victor Popov, Nikolai Medvedev, Paul Giabbon, Nicola Corbett, Igor Kraev, Heather Davies. -- Part VIII. Orthologs and paralogs of NCAM. -- Fasciclin II: the NCAM ortholog in Drosophila melanogaster / Lars V. Kristiansen, Michael Hortsch. -- Neural cell adhesion molecule NCAM2/OCAM/RNCAM, a close relative to NCAM / Nikolaj Kulahin, Peter S. Walmod. -- Part IX. Conclusion. -- Honoring Dr. Elisabeth Bock / Vladimir Berezin.
  • Chapter 1. Lipid structure -- Chapter 2. The mesomorphic phase behavior of lipid bilayers -- Chapter 3. Lipid bilayer interdigitation -- Chapter 4. The dynamics of membrane lipids -- Chapter 5. Nonlamellar lipid phases -- Chapter 6. The forces between interacting bilayer membranes and the hydration of phospholipid assemblies -- Chapter 7. The roles of cholesterol in the biology of cells -- Chapter 8. Lipid membrane fusion -- Chapter 9. Membrane rafts -- Chapter 10. Passive and facilitated transport -- Chapter 11. Membrane protein dynamics: rotational dynamics -- Chapter 12. Translational diffusion of membrane proteins -- Chapter 13. Inorganic anion transporter AE1 -- Chapter 14. The structures of G-protein coupled receptors -- Chapter 15. Role of membrane lipids in modulating the activity of membrane-bound enzymes -- Chapter 16. Viral fusion mechanisms.
  • Joint diseases are a major public health concern. Reactive oxygen species are mediators in pathways associated with joint tissue injury. Oxidative Stress in Applied Basic Research and Clinical Practice: Studies on Arthritis and Joint Disorders provides background to understanding the role of reactive oxygen species in joint diseases, as well as therapeutic strategies for their regulation. International experts discuss recent advances on experimental and clinical data focused on free radical-mediated mechanisms of disease. The book also addresses the protective role of antioxidants against the insult of reactive oxygen species in rheumatic diseases.
  • Introduction -- Cytology of the subthalamic nucleus -- Ontogeny of the subthalamic nucleus -- Topography of the rat, cat, baboon, and human subthalamic nucleus -- Connections of the subthalamic nucleus -- Nigro-subthalamic connections in rat.
  • Cell engineering with synthetic messenger RNA / Peter M. Rabinovich and Sherman M. Weissman -- In vitro transcription of long RNA containing modified nucleosides / Norbert Pardi [and others] -- HPLC purification of in vitro transcribed long RNA / Drew Weissman [and others] -- Synthetic mRNAs with superior translation and stability properties / Ewa Grudzien-Nogalska [and others] -- Nonviral, cationic lipid-mediated delivery of mRNA / James G. Hecker -- Light-induced mRNA transfection / Sigurd Leinæs Bøe and Eivind Hovig -- Engineering B cells with mRNA / Jaewoo Lee, David Boczkowski, and Smita Nair -- Programming human dendritic cells with mRNA / Jaewoo Lee, David Boczkowski, and Smita Nair -- Large volume flow electroporation of mRNA : clinical scale process / Linhong Li [and others] -- Retrovirus-based mRNA transfer for transient cell manipulation / Melanie Galla, Axel Schambach, and Christopher Baum -- mRNA delivery to human dendritic cells by recombinant yeast and activation of antigen-specific memory T cells / Frank Breinig, Tanja Breinig, and Manfred J. Schmitt -- Nonviral RNA transfection to transiently modify T cells with chimeric antigen receptors for adoptive therapy / Tobias Riet [and others] -- Natural killer cell reprogramming with chimeric immune receptors / Noriko Shimasaki and Dario Campana -- Reprogramming to pluripotency and differentiation of cells with synthetic mRNA / Peizhe Wang and Jie Na -- Antitumor vaccination with synthetic mRNA : strategies for in vitro and in vivo preclinical studies / Mustafa Diken [and others] -- Mannosylated and histidylated LPR technology for vaccination with tumor antigen mRNA / Chantal Pichon and Patrick Midoux -- Analysis of survivin-specific T cells in breast cancer patients using human DCs engineered with survivin mRNA / Ozcan Met and Inge Marie Svane -- mRNA electroporation as a tool for immunomonitoring / Nathalie Cools [and others] -- mRNA PCR-based epitope chase method / Jean-Daniel Doucet, Dominique Gauchat, and Rejean Lapointe.
  • Print Material
    Molecular and Cellular Biology: An Engineering Perspective. Analysis: Signal Processing. Analysis: Control and Systems. Analysis: Probabilistic Data Networks and Communications. Design: Synthetic Biology. Integration: Reverse Engineering from Biology.
  • The objective of this book is to present the strategies employed by living organisms on a molecular level and to help understand the basics of Systems Biology. Its content is organized in a way to meet the exponential growth in the volume of biological knowledge, and the need for a multidisciplinary approach in the practice of teaching modern biology. For this reason, the whole material is divided into five chapters, each devoted to a fundamental concept: Structure-Function, Energy, Information, Regulation and Interrelationships. The book describes generic mechanisms which occur in biology and promotes a simulation-based approach to the subject of Systems Biology. The use of basic knowledge as the background for presenting biological problems obligates the teachers to deal with generalized phenomena comprising the ever increasing volume of teaching materials.
  • Also available: Print – 2014
    Overview of orchestration of CD4+ T cell subsets in immune responses -- Th1/Th2 cell differentiation and molecular signals -- Th1/Th2 cell?s function in immune system -- Regulatory T Cell differentiation and their function in immune regulation -- Th17 differentiation and their pro-inflammation function -- Tfh Cell differentiation and their function in promoting B cell responses -- Differentiation, Regulation and Function of Th9 Cell -- The biology and functions of Th22 cell.
  • Introduction to telomeres and telomerase / Z. Songyang -- Analysis of average telomere length in cultured human cells / D. Liu -- Telomere length analysis by quantitative fluorescent in situ hybridization (Q-FISH) / I. Ourliac-Garnier and A. Londono-Vallejo -- Telomere strand-specific length analysis by fluorescent in situ hybridization (Q-CO-FISH) / I. Ourliac-Garnier and A. Londono-Vallejo -- Telomere G-overhang length measurement method 1: The DSN method / Y. Zhao, J.W. Shay and W.E. Wright -- Telomere G-overhang length measurement method 2: G-tail telomere HPA / H. Tahara -- Telomere terminal G/C strand synthesis: Measuring telomerase action and C-RICH fill-in / Y. Zhao, J.W. Shay and W.E. Wright -- G-quadruplex structures and G-quadruplex-interactive compounds / R.I. Mathad and D. Yang -- Analysis of yeast telomerase by primer extension assays / M. Hsu and N.F. Lue -- Telomeric repeat amplification protocol: Measuring the activity of the telomerase / H. Xin -- CO-FISH, COD-FISH, ReD-FISH, SKY-FISH / E.S. Williams [and others] -- Visualization of human telomerase localization by fluorescence microscopy techniques / E. Abreu, R.M. Terns and M.P. Terns -- Cytogenetic analysis of telomere dysfunction / A.S. Multani and S. Chang -- Probing the telomere damage response / R. Rai and S. Chang -- Analysis of telomere proteins by chromatin immunoprecipitation (CHIP) / W. Ma -- Studying of telomeric protein-protein interactions by bi-molecular fluorescence complementation (BIFC) and peptide array-based assays / W. Ma, H. Kim and Z. Songyang -- Human telomere POT1-TPP1 complex and its role in telomerase activity regulation / F. Wang and M. Lei.
  • Also available: Print – 2013
    Introduction: The GW Body Story as an Example of Autoantibodies with Significant Impacts to Molecular Cell Biology -- The Discovery of GW Bodies -- The Discovery and Analysis of P Bodies -- Autoantibodies to Argonaute 2 (Su Antigen) -- GW/P-Bodies and Autoimmune Disease -- Function of GW182 and GW Bodies in siRNA and miRNA Pathways -- Post-transcriptional Stimulation of Gene Expression by MicroRNAs -- Gawky (GW) is the Drosophila melanogaster GW182 Homologue -- The Role of GW182 Proteins in miRNA-Mediated Gene Silencing -- Quantifying Argonaute Proteins In and Out of GW/P-Bodies: Implications in microRNA Activities -- Deadenylation and P-Bodies -- Relationship of GW/P-Bodies with Stress Granules -- Relationship of Other Cytoplasmic Ribonucleoprotein Bodies (cRNPB) to GW/P Bodies -- An SNP in the Trinucleotide Repeat Region of the TNRC6A Gene Maps to a Major TNGW1 Autoepitope in Patients with Autoantibodies to GW182 -- Reflections on Ten Years of History of, and Future Prospects for, GW182 and GW/P Body Research.
  • This volume provides a brief resume of the history of testosterone research, from the early pioneers through to the most recent advances in the field. We discover how steroid hormones were first identified and how testosterone was shown to be essential for male development. Moving forward we explore how and where testosterone is produced, and how the body controls testosterone production. We then investigate the impact testosterone has on different body systems both during their development and function, and how perturbation of testosterone action is associated with disease. We complete our story with an exploration of the emerging roles of testosterone in clinical therapy, and the future potential for manipulation of the testosterone signaling system for human health benefit.
  • Angiogenesis and lymphangiogenesis have become attractive targets for drug therapy because of their key roles in a broad spectrum of pathological disease states ranging from macular degeneration to tumor growth and metastasis. A substantial increase in the research effort over the past decade has deepened our understanding of the basic mechanisms underlying angiogenesis and lymphangiogenesis, promoting the development of promising therapeutics for the clinical management of vascular-related diseases.
  • Also available: Print – 2009
  • Also available: Print – 2010
    Engineering of Fluorescent Reporters into Redox Domains to Monitor Electron Transfers / Derek Parsonage ... [et al.] -- Blot-Based Detection of Dehydroalanine-Containing Glutathione Peroxidase with the Use of Biotin-Conjugated Cysteamine / Sue Goo Rhee, Chun-Seok Cho -- Analysis of the Redox Regulation of Protein Tyrosine Phosphatase Superfamily Members Utilizing a Cysteinyl-Labeling Assay / Benoit Boivin, Nicholas K. Tonks -- Measuring the Redox State of Cellular Peroxiredoxins by Immunoblottin / Andrew G. Cox, Christine C. Winterbourn, Mark B. Hampton -- Thiol Redox Transitions by Thioredoxin and Thioredoxin-Binding Protein-2 in Cell Signaling / Eiji Yoshihara ... [et al.] -- Detection of Protein Thiols in Mitochondrial Oxidative Phosphorylation Complexes and Associated Proteins / Kelly K. Andringa, Shannon M. Bailey -- Mitochondrial Thioredoxin Reductase: Purification, Inhibitor Studies, and Role in Cell Signaling / Maria Pia Rigobello, Alberto Bindoli -- Measuring Mitochondrial Protein Thiol Redox State / Raquel Requejo ... [et al.] -- Measurement of Extracellular (Exofacial) Versus Intracellular Protein Thiols / Jolanta Skalska, Steven Bernstein, Paul Brookes -- Redox Clamp Model for Study of Extracellular Thiols and Disulfides in Redox Signaling / Young-Mi Go, Dean P. Jones -- Redox State of Human Serum Albumin in Terms of Cysteine-34 in Health and Disease / Karl Oettl, Gunther Marsche -- Methods for Studying Redox Cycling of Thioredoxin in Mediating Preconditioning-Induced Survival Genes and Proteins / Chuang C. Chiueh -- Oxidative Stress, Thiol Redox Signaling Methods in Epigenetics / Isaac K. Sundar ... [et al.] -- Characterization of Protein Targets of Mammalian Thioredoxin Reductases / Anton A. Turanov, Dolph L. Hatfield, Vadim N. Gladyshev -- Alteration of Thioredoxin Reductase 1 Levels in Elucidating Cancer Etiology / Min-Hyuk Yoo ... [et al.] -- Regulation of Apoptosis Signal-Regulating Kinase 1 in Redox Signaling / Kazumi Katagiri, Atsushi Matsuzawa, Hidenori Ichijo -- Protocols for the Detection of S-Glutathionylated and S-Nitrosylated Proteins In Situ / Scott W. Aesif, Yvonne M.W. Janssen-Heininger, Niki L. Reynaert -- Synthesis, Quantification, Characterization, and Signaling Properties of Glutathionyl Conjugates of Enals / Sanjay Srivastava ... [et al.] -- Thioredoxin and Redox Signaling in Vasculature--Studies Using Trx2 Endothelium-Specific Transgenic Mice / Wang Min ... [et al.]
  • Tight junctions 2006
    Evolution of the transporting epithelium phenotype / Marcelino Cereijido ... [et al.] -- Occludin, a constituent of tight junctions / Yan-Hua Chen, Daniel A. Goodenough and Qun Lu -- Tight junction channels / James Melvin Anderson and Christina M. Van Itallie -- JAM family proteins: tight junction proteins that belong to the immunoglobulin superfamily / Susumu Hirabayashi and Yutaka Hata -- Cingulin, a cytoskeleton-associated protein of the tight junction / Laurent Guillemot and Sandra Citi -- ZO proteins and tight junction assembly / Alan S. Fanning -- TJ proteins that make round trips to the nucleus / Esther Lopez-Bayghen ... [et al.] -- Tight junctions and the regulation of epithelial -- Cell proliferation and gene expression / Emma Kavanagh ... [et al.] -- Tight junction proteins and cancer / Isabel J. Latorre, Kristopher K. Frese and Ronald T. Javier -- Regulation of paracellular transport across tight junctions by the actin cytoskeleton / Matthias Bruewer and Asma Nusrat -- Regulation of tight junctions' functional integrity: role of a urinary factor, lipids, and ouabain / Liora Shoshani ... [et al.] -- Tight junctions during development / Bhavwanti Sheth ... [et al.] -- Tight junctions and the blood-brain barrier / Hartwig Wolburg, Andrea Lippoldt, and Klaus Ebnet -- Tight junctions in CNS myelin / Jeff M. Bronstein and Seema Tiwari-Woodruff -- Tight junction modulation and its relationship to drug delivery / Noha N. Salama, Natalie D. Eddington and Alessio Fasano.
  • 1. Cytomechanics in connective tissue repair and engineering / Robert A. Brown -- 2. Scleroderma lung fibroblasts : contractility and connective tissue growth factor / Galina S. Bogatkevich ... [et al.] -- 3. Functional assessment of fibroblast heterogeneity by the cell-surface glycoprotein thy-1 / Carolyn J. Baglole ... [et al.] -- 4. Tissue repair in asthma : the origin of airway subepithelial fibroblasts and myofibroblasts / Sabrina Mattoli -- 5. Experimental models to study the origin and role of myofibroblasts in renal fibrosis / Michael Zeisberg, Mary A. Soubasakos and Raghu Kalluri -- 6. Epithelial to mesenchymal transition of mesothelial cells as a mechanism responsible for peritoneal membrane failure in peritoneal dialysis patients / Abelardo Aguilera ... [et al.] -- 7. FIZZy alveolar epithelial cells induce myofibroblast differentiation / Sem H. Phan -- 8. Pro-invasive molecular cross-signaling between cancer cells and myofibroblasts / Olivier De Wever and Marc Mareel -- 9. Proangiogenic implications of hepatic stellate cell transdifferentiation into myofibroblasts induced by tumor microenvironment / Elvira Olaso ... [et al.] -- 10. Matrix metalloproteinases, tissue inhibitors of metalloproteinase and matrix turnover and the fate of hepatic stellate cells / Aqeel M. Jamil and John P. Iredale -- 11. Innate immune regulation of lung injury and repair / Dianhua Jiang ... [et al.] -- 12. An Eye on repair : myofibroblasts in corneal wounds / James V. Jester.
  • TNF is a multifunctional proinflammatory cytokine central to the development and homeostasis of the immune system and a regulator of cell activation, differentiation and death. Recent decades have seen an enormous scientific and clinical interest in the function of TNF in physiology and disease. A vast amount of data has been accumulated at the biochemical, molecular and cellular level, establishing TNF as a prototype for in-depth understanding of the physiological and pathogenic functions of cytokines. This volume covers several current aspects of TNF regulation and function, including transcriptional and posttranscriptional control mechanisms, cellular modes of action, signaling networks that mediate its effect, involvement in pathogenesis and clinical outcomes of TNF antagonists. It combines basic science at the molecular and cellular level with research in animal models of disease and clinical findings to provide a comprehensive review of recent developments in TNF biology. A thorough understanding of the mechanisms by which this key molecular player is produced and functions to regulate cell biology, immunity and disease postulates novel paradigms on how genes contribute to the development and physiology of biological systems.
  • Comprehensive overview of different aspects of autophagy as it relates to neurodegenerative diseases. The pathogenesis of the main neurodegenerative disorders includes either the accumulation of altered or misfolded proteins or exposure to several toxics. Autophagy constitute one of the two principal cellular pathways implicate in the clearance of these material and can serve as a neuroprotective mechanism. Topics include: the role of autophagy in the brain, the role of autophagy in the principal neurodegenerative disorders, and the mechanism by which different molecules cause neurotoxicity and the role autophagy plays.
  • A review of post-translational modifications and subcellular localization of Ets transcription factors : possible connection with cancer and involvement in the hypoxic response / Céline Charlot ... [et al.] -- Regulation of transcription factor function by targeted protein degradation : an overview focusing on p53, c-Myc, and c-Jun / Jukka Westermarck -- Review of molecular mechanisms involved in the activation of the Nrf2-ARE signaling pathway by chemopreventive agents / Aldo Giudice, Claudio Arra, and Maria C. Turco -- Subnuclear localization and intranuclear trafficking of transcription factors / Sayyed K. Zaidi ... [et al.] -- Analysis of ligand-dependent nuclear accumulation of Smads in TGF-[beta] signaling / Douglas A. Chapnick and Xuedong Liu -- Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1 / Richard Y.M. Ma ... [et al.] -- Coupling of dephosphorylation and nuclear export of Smads in TGF-[beta] signaling / Fangyan Dai ... [et al.] -- Assessing sequence-specific DNA binding and transcriptional activity of STAT1 transcription factor / Thomas Meyer and Uwe Vinkemeier -- Analysis of nuclear export using photoactivatable GFP fusion proteins and interspecies heterokaryons / Kerry-Ann Nakrieko, Iordanka A. Ivanova, and Lina Dagnino -- Determination of nuclear localization signal sequences for Krüppel-like factor 8 / Tina S. Mehta, Farah Monzur, and Jihe Zhao -- Methods to measure nuclear export of [beta]-catenin using fixed and live cell assays / Manisha Sharma and Beric R. Henderson -- Imaging of transcription factor trafficking in living cells : lessons from corticosteroid receptor dynamics / Mayumi Nishi -- Hypoxia-inducible factors : post-translational crosstalk of signaling pathways / Elitsa Y. Dimova and Thomas Kietzmann -- The basic helix-loop-helix-leucine zipper gene Mitf : analysis of alternative promoter choice and splicing / Kapil Bharti ... [et al.] -- Phosphorylation control of nuclear receptors / Sébastien Lalevée, Christine Ferry, and Cécile Rochette-Egly -- Regulation of Krüpple-like [i.e. Krüppel-like] factor 5 by targeted protein degradation / Ceshi Chen -- Post-translational control of ETS transcription factors : detection of modified factors at target gene promoters / Li Li, Janice Saxton, and Peter E. Shaw -- Integration of protein kinases into transcription complexes : identifying components of immobilised in vitro pre-initiation complexes / Hong-Mei Zhang ... [et al.] -- Post-translational modification of p53 by ubiquitin / Chunhong Yan -- Phosphorylation-dependent regulation of SATB1, the higher-order chromatin organizer and global gene regulator / Dimple Notani ... [et al.] -- In vivo and in vitro tools to identify and study transcriptional regulation of USF-1 target genes / Marie-Dominique Galibert and Sébastien Corre -- Measuring the absolute abundance of the Smad transcription factors using quantitative immunoblotting / David C. Clarke and Xuedong Liu -- Flow cytometry analysis of transcription factors in T lymphocytes / Diana I. Albu, Danielle Califano, and Dorina Avram -- Identification of specific protein/E-box-containing DNA complexes : lessons from the ubiquitously expressed USF transcription factors of the b-HLH-LZ super family / Marie-Dominique Galibert and Yorann Baron.
  • Also available: Print – 2013
    1. The Stem cell state / Gary R. Hime, Helen E. Abud -- 2. Induction of pluripotency / Corey Heffernan, Jun Liu, Huseyin Sumer -- pt. I. Model stem cell systems (A): invertebrate -- 3. Germline stem cells and their regulation in the nematode Caenorhabditis elegans / Aaron Kershner, Sarah L. Crittenden, Kyle Friend, Erika B. Sorensen, Douglas F. Porter -- 4.Transcriptional and post-transcriptional regulation of Drosophila germline stem cells and their differentiating progeny / Helen White-Cooper, Simona Caporilli -- 5. Stem cells in the Drosophila digestive system / Xiankun Zeng, Chhavi Chauhan, Steven X. Hou -- 6. Mechanisms of asymmetric progenitor divisions in the Drosophila central nervous system / Rita Sousa-Nunes, W. Gregory Somers -- pt. II. Model stem cell systems (B): vertebrate -- 7. Transcriptional/translational regulation of mammalian spermatogenic stem cells / Cathryn A. Hogarth -- 8. Transcriptional regulation and specification of neural stem cells / Kimberly J. Christie, Ben Emery, Mark Denham, Helena Bujalka, Holly S. Cate -- 9. Transcriptional control of epidermal stem cells / Briana Lee, Xing Dai -- 10. Regulation of intestinal stem cells by WNT and notch signalling / Katja Horvay, Helen E. Abud -- 11. Transcriptional regulation of haematopoietic stem cells / Adam C. Wilkinson, Berthold Göttgens -- 12. The Regulation of mesenchymal stem cell differentiation / David Cook, Paul Genever -- pt. III. Molecular families implicated in stem cell regulation -- 13. The Musashi family of RNA binding proteins: master regulators of multiple stem cell populations / Jessie M. Sutherland, Eileen A. McLaughlin, Gary R. Hime, Nicole A. Siddall -- 14. JAK-STAT signaling in stem cells / Rachel R. Stine, Erika L. Matunis -- 15. Myc in stem cell behaviour: insights from Drosophila / Leonie M. Quinn, Julie Secombe, Gary R. Hime -- 16. The Role of nuclear receptors in embryonic stem cells / Qin Wang, Austin J. Cooney -- 17. Epigenetic regulation of stem cells / Anton Wutz -- 18. Regulation of stem cell populations by microRNAs / Julie Mathieu, Hannele Ruohola-Baker -- 19. Myb and the regulation of stem cells in the intestine and brain: a tale of two niches / Jordane Malaterre, Lloyd Pereira, Robert G. Ramsay.
  • Insights into the regulation of immune cell lineage differentiation and specification as well as into the control of lineage integrity, stability and plasticity are of fundamental importance to understanding innate and adaptive immune responses. In this volume, leading experts provide an up-to-date and comprehensive overview of recent advances in the transcriptional control mechanisms and transcription factor networks that regulate these processes in a variety of different immune cell lineages. The chapters cover the regulation of T versus B cell lineage choice, discuss early B cell development and pre-B cell leukemia prevention, address transcriptional control mechanisms during Th differentiation, in regulatory T cells and iNKT cells, detail genomic switches in helper cell fate choice and plasticity, and highlight the role of the BTB-zinc finger family of transcription factors in T cells. Moreover, the chapters discuss transcriptional networks in DCs, NK cells and in innate lymphoid cells. Together, the reviews illustrate key transcriptional control mechanisms that regulate the development and function of immune cells and demonstrate the impressive advances made over the last decade.
  • "Cell signaling, which is also often referred to as signal transduction or, in more specialized cases, transmembrane signaling, is the process by which cells communicate with their environment and respond temporally to external cues that they sense there. All cells have the capacity to achieve this to some degree, albeit with a wide variation in purpose, mechanism, and response. At the same time, there is a remarkable degree of similarity over quite a range of species, particularly in the eukaryotic kingdom, and comparative physiology has been a useful tool in the development of this field. The central importance of this general phenomenon (sensing of external stimuli by cells) has been appreciated for a long time, but it has truly become a dominant part of cell and molecular biology research in the past three decades, in part because a description of the dynamic responses of cells to external stimuli is, in essence, a description of the life process itself. This approach lies at the core of the developing fields of proteomics and metabolomics, and its importance to human and animal health is already plainly evident"--Provided by publisher.
  • v. 2 2008 Springer
    v. 1. Basic and clinical biology -- v. 2. Cancer treatment and therapy.
  • Also available: Print – 2007
    Purification of FLAG-tagged eukaryotic initation factor 2B complexes, subcomplexes, and fragments from Saccharomyces cerevisiae / Sarah S. Mohammad-Qureshi ... [et al.] -- In vivo deletion analysis of the architecture of a multiprotein complex of translation initiation factors / Klaus H. Nielsen and Leos̆ Valás̆ek -- An approach to studying the localization and dynamics of eukaryotic translation factors in live yeast cells / Susan G. Campbell and Mark P. Ashe -- In vitro and tissue culture methods for analysis of translation initiation on the endoplasmic reticulum / Samuel B. Stephens and Christopher V. Nicchitta -- Mammalian stress granules and processing bodies / Nancy Kedersha and Paul Anderson -- Methods to analyze micro-RNA-mediated control of mRNA translation / Jennifer L. Clancy ... [et al.] -- Methods for studying signal-dependent regulation of translation factor activity / Xuemin Wang and Christopher G. Proud -- Analysis of mRNA translation in cultured hippocampal neurons / Yi-Shuian Huang and Joel D. Richter -- Detecting ribosomal association with the 5' leader of mRNAs by ribosome density mapping (RDM) / Naama Eldad and Yoav Arava -- Genome-wide analysis of mRNA polysomal profiles with spotted DNA microarrays / Danial Melamed and Yoad Arava -- Synthesis of anti-reverse cap analogs (ARCAs) and their applications in mRNA translation and stability / Ewa Grudzien-Nogalska ... [et al.] -- Methods for identifying compounds that specifically target translation / Letizia Brandi ... [et al.] -- Identifying small molecule inhibitors of eukaryotic translation initiation / Regina Cencic, Francis Robert, and Jerry Pelletier -- Isolation and identification of eukaryotic initiation factor 4A as a molecular target for the marine natural product pateamine A / Woon-Kai Low ... [et al.].
  • For many years, the ethical discussion surrounding human embryonic stem cell research has focused on the moral status of the embryo. This text takes a wider moral berth and focuses on numerous ethical, legal and social aspects involved in translating the results of stem cell research into diagnostic and therapeutic applications.
  • The establishment of composite tissue allotransplantation as a clinical reality / Joseph E. Murray -- The evolution of composite tissue allotransplantation: the twentieth century realization of "Cosmas and Damian" / Chad R. Gordon ... [et al.] -- The impact of the discovery of cyclosporine on transplantation of composite tissue allografts / Zsolt T. Csapo, Bence Forgacs, and Barry D. Kahan -- The first limb transplants with cyclosporine / Christopher J. Salgado, Samir Mardini, and Charles W. Hewitt -- Translational research in composite tissue allotransplantation / Linda C. Cendales, David E. Kleiner, and Allan D. Kirk -- Relative antigenicity of allograft components and differential rejection / Jignesh Unadkat ... [et al.] -- On the road to tolerance induction in composite tissue allotransplantation / Mark A. Hardy -- Donor-specific tolerance / Au H. Bui, Gerald Lipshutz, and Jerzy Kupiec-Weglinski -- Immune tolerance induction: basic concepts for composite tissue allotransplantation / Patricio Andrades, Clement Asideu, and Judith M. Thomas -- Allograft survival with calcineurin inhibitors / Neil F. Jones and Esther Voegelin -- Long-term prevention of rejection and combination drug therapy / Thomas H. Tung -- Locoregional immunosuppression in composite tissue allografting / Scott A. Gruber -- New approaches to antibody therapy / Dalibor Vasilic, Moshe Kon, and Cedric G. Francois -- World experience of hand transplantation-independent assessment / W.P. Andrew Lee, Justin M. Sacks, and Elaine K. Horibe -- Hand transplantation: Lyon experience / Palmina Petruzzo ... [et al.] -- Hand transplantation: the Louisville experience / Vijay S. Gorantla and Warren C. Breidenbach III -- Hand transplantation: the Innsbruck experience / Stefan Schneeberger, Marina Ninkovic, and Raimund Margreiter -- Vascularized bone marrow transplantation / Chau Y. Tai ... [et al.] -- Vascularized bone marrow transplantation: pathology of composite tissue transplantation-induced graft-versus-host-disease / Rajen Ramsamooj and Charles W. Hewitt -- Immune cell redistribution after vascularized bone marrow transplantation / Waldemar L. Olszewski and Marek Durlik -- Vascularized knee transplantation / Michael Diefenbeck and Gunther O. Hofmann -- Tracheal transplantation / Gabriel M. Marta and Walter Klepetko -- Laryngeal transplantation / Robert R. Lorenz and Marshall Strome -- Face transplantation / Maria Siemionow and Galip Agaoglu -- The role of allografts in lower extremity reconstruction / Milton B. Armstrong, Ricardo Jimenez-Lee, and Eddie Manning -- Skin allografts in scalp reconstruction / Peter C. Neligan -- Abdominal wall transplantation / Chau Y. Tai and Susan E. Mackinnon -- Live-donor nerve transplantation / Scott A. Gruber and Pedro Mancias -- Ethical and policy concerns of hand/face transplantation / Rhonda Gay Hartman -- Ethical debate on human face transplantation / François Petit -- Psychosocial issues in composite tissue allotransplantation / Barckley Storey ... [et al.] -- Composite tissue transplantation in the twenty-first century / Kirby S. Black and Charles W. Hewitt.
  • Transport in Biological Media is a solid resource of mathematical models for researchers across a broad range of scientific and engineering problems such as the effects of drug delivery, chemotherapy, or insulin intake to interpret transport experiments in areas of cutting edge biological research. A wide range of emerging theoretical and experimental mathematical methodologies are offered by biological topic to appeal to individual researchers to assist them in solving problems in their specific area of research. Researchers in biology, biophysics, biomathematics, chemistry, engineers and clinical fields specific to transport modeling will find this resource indispensible. Provides detailed mathematical model development to interpret experiments and provides current modeling practices Provides a wide range of biological and clinical applicationsIncludes physiological descriptions of models.
  • Part I. Molecular bases for stem and cancer cells proliferation -- An introduction to proliferation and migration of stem cells and cancer -- the role of cell adhesions, cell junctions and extracellular matrix in normal development and carcinogenesis -- The embryonic rest hypothesis of cancer development: 150 years later -- Are calcium channels more important than calcium influx for cell proliferation?- Role of calcium signaling in stem cells and cancer cells proliferation -- Formation and function of lipid droplets in inflammation and cancer -- The role of sphingolipids in modulating pluripotency of stem cells -- Part II. Signaling pathways to cancer cells fate -- Signaling pathways of cbl-b and its role on peripheral t cell tolerance -- Bh3-only proteins in cancer and apoptosis -- Signaling pathways of mta family proteins as regulators of cancer progression and metastasis -- Signalling pathways of [beta]-catenin/jnk on carcinogenesis -- The regulation of the jnk cascade and programmed cell death by nf- b: mechanisms and functions -- Part III. Uncontrolled physiology process guiding to cancer cells -- Migration, metastasis and more: the role of chemokines in the proliferation, spreading and metastasis of tumors -- Autophagy, cell death, and cancer -- The duality of stem cells: double edged sword in tumour evolution and treatment -- Glioblastoma stem cells and implications for chemoresistance -- The tumor microenvironment as a transient niche: a modulator of epigenetic states and stem cell functions -- Tumor-associated neutrophils (tans) -- Microrna control of apoptotic programs in cancer -- Part IV. Therapeutic strategies for anticancer treatment -- Markers of cancer stem cells and their functions -- Cancer cell dormancy: potential therapeutic targets to eradicate cancer cells within the niche -- Modulating apoptosis in cancer therapy with ultrasound and high intensity nanosecond electric pulses -- Targeting cell cycle for anticancer treatment and neuroprotection.
  • Also available: Print – 2014
    The collection of chapters in this proceeding volume reflects the latest research presented at the Aegean meeting on Tumor Microenvironment and Cellular Stress held in Crete in Fall of 2012. The book provides critical insight to how the tumor microenvironment affects tumor metabolism, cell stemness, cell viability, genomic instability and more. Additional topics include identifying common pathways that are potential candidates for therapeutic intervention, which will stimulate collaboration between groups that are more focused on elucidation of biochemical aspects of stress biology and groups that study the pathophysiological aspects of stress pathways or engaged in drug discovery.
  • Two-component signaling systems. Part A-C pt. A-B, C, 2007, 2010.
    pt. B, 2007 ScienceDirect
    pt. C, 2010 ScienceDirect
    Pt. A. Computational analyses of sequences and sequence alignments ; Biochemical and genetic assays of individual components of signaling systems ; Physiological assays and readouts -- Pt. B. Structural approaches ; Reconstritution of heterogeneous systems ; Intracellular methods and assays ; Genome-wide analyses of two-component systems.
  • pt. B ScienceDirect
    pt. C ScienceDirect
    Also available: Print – pt. A-C, 2011.
  • There is now compelling evidence that the complexity of higher organisms correlates with the relative amount of non-coding RNA rather than the number of protein-coding genes. Previously dismissed as ""junk DNA"", it is the non-coding regions of the genome that are responsible for regulation, facilitating complex temporal and spatial gene expression through the combinatorial effect of numerous mechanisms and interactions working together to fine-tune gene expression. The major regions involved in regulation of a particular gene are the 5' and 3' untranslated regions and introns.
  • Print Material
    Section I. Histology and Cell Biology -- Section II. Early Embryology -- Section III. Gross Anatomy -- Section IV. Neuroscience -- Index.
  • Also available: Print – 2009
    Part I. Pandemic Influenza Overview -- Pandemic influenza as a current threat -- Antigenic cross-reactivity among H5N1 Viruses -- -- Part II. Current Approaches for Human and Avian Vaccine Production -- Seasonal influenza vaccines -- Generation and characterization of candidate vaccine viruses for pre-pandemic influenza vaccines -- Live attenuated vaccines for pandemic influenza -- Influenza vaccines for avian species -- Development and application of avian influenza vaccines in China -- -- Part III. Novel Vaccine Approaches -- Designing vaccines for pandemic influenza -- Attenuated influenza virus vaccines with modified NS1 proteins -- DNA Vaccines against Influenza viruses -- Recombinant proteins produced in insect cells -- Influenza neuraminidase as a vaccine antigen -- Recombinant vectors as influenza vaccines -- Influenza virus-like particles as pandemic vaccines -- Pandemic influenza vaccines -- Adjuvants for pandemic influenza vaccines -- -- Part IV. Novel Approaches of Vaccine Delivery -- Transcutaneous immunization with influenza vaccines -- Microneedle-based vaccines -- -- Part V. Vaccine Evaluation, Production and Distribution -- Animal models for evaluation of influenza vaccines -- Immunosenescence and influenza vaccine efficacy -- Vaccines for pandemic influenza: summary of recent clinical trials -- Considerations for licensure of influenza vaccines with pandemic and pre-pandemic indications -- Strategies for broad global access to pandemic influenza -- Prioritization of pandemic influenza vaccine: rationale and strategy for decision-making.
  • Also available: Print – 2015
    This book addresses the differentiation control of skeletal muscle in different locations of the vertebrate body. Particular attention is paid to novel regulatory molecules and signals as well as to the heterogeneity of origin that have revealed a developmental overlap between skeletal and cardiac muscle. Different functional muscle groups are the product of the evolution of the vertebrate classes, making a phylogenetic comparison worthwhile for understanding the role of muscle stem cells and precursors in myogenesis. New insights into the hierarchy of transcription factors, particularly in the context of these different muscle groups come from detailed investigations of the spatio-temporal and regulatory relationships derived from mouse and zebrafish genetics and avian microsurgery. Importantly, epigenetic mechanisms that have surfaced recently, in particular the role of MyomiRs, are also surveyed. Regarding human patients, encouraging results have been generated that identify parallels between embryonic myogenesis and regenerating myofibers that share regulatory molecules. Interestingly, the heterogeneity in embryonic origins of skeletal muscle groups in the vertebrate including humans is paralleled by their different susceptibility to types of muscle dystrophies. The progress that has been made in the field of muscle stem cell biology, especially on satellite cells, is outlined in this book by experts in the field. The authors review recent insights of the heterogeneous nature of these satellite cells regarding their gene signatures and regeneration potential. An improved understanding of muscle stem cells seems only possible with a view to the cell environment, putting embryological and molecular findings from different vertebrate classes and stem cell approaches into context.
  • Clathrin-Mediated Endocytosis / Alexander Sorkin, Manojkumar A. Puthenveedu -- Intimate and Facultative? Regulation of Clathrin-Mediated Endocytosis by the Actin Cytoskeleton / Tal Hirschhorn, Marcelo Ehrlich -- Oncogenic Signaling from the Plasma Membrane / Eli Zamir, Nachiket Vartak, Philippe I. H. Bastiaens -- Endocytosis in the Spatial Control of Polarised Cell Functions / Giorgio Scita, Andrea Disanza, Emanuela Frittoli -- Aberrant Vesicular Trafficking Contributes to Altered Polarity and Metabolism in Cancer / Shreya Mitra, Gordon B. Mills -- Endocytosis and the Regulation of Cell Signaling, Cell Adhesion, and Epithelial to Mesenchymal Transition in Cancer / Crislyn D'Souza-Schorey, Guangpu Li -- Efficient Enhancement of Signaling Capacity: Signaling Endosomes / Iwona Pilecka, Marta Miaczynska -- Nuclear Functions and Trafficking of Receptor Tyrosine Kinases / Ying-Nai Wang, Jennifer L. Hsu, Mien-Chie Hung -- Efficient Enhancement of Signalling Capacity: The Ubiquitin System / Daniela Hoeller, Ivan Dikic -- Molecular Mechanism of Ubiquitin-Dependent Traffic / Elena Maspero, Hans-Peter Wollscheid, Simona Polo -- Cbl as a Master Regulator of Receptor Tyrosine Kinase Trafficking / Ke Ma, Stephen C. Kales, Marion M. Nau, Stanley Lipkowitz -- Regulation of Endocytic Trafficking and Signalling by Deubiquitylating Enzymes / Han Liu, Sylvie Urbé, Michael J. Clague -- RTKs as Models for Trafficking Regulation: c-Met/HGF Receptor-c-Met Signalling in Cancer--Location Counts / Carine Joffre, Rachel Barrow, Ludovic Ménard -- Regulation of Epidermal Growth Factor Receptor Signaling by Endocytosis in Normal and Malignant Cells / Sergio Anastasi, Stefano Alemà, Oreste Segatto MD -- Stress-Driven Endocytosis of Tyrosine-Phosphorylated EGFR Leads to Tumorigenesis: The Critical Role of Oxidative Stress / Tzipora Goldkorn, Simone Filosto, Samuel Chung -- Internalisation, Endosomal Trafficking and Recycling of Integrins During Cell Migration and Cancer Invasion / Elena Rainero, Peter V. E. van den Berghe, Jim C. Norman -- Antibody-Mediated Receptor Endocytosis: Harnessing the Cellular Machinery to Combat Cancer / Gabi Tarcic, Yosef Yarden.
  • Also available: Print – 2013
    For a virus to invade a host cell it needs to penetrate the physical barrier imposed by the plasma membrane. Viruses have evolved specialized surface proteins to meet this challenge. These proteins facilitate delivery of the viral genetic information into the host cell by either fusing the viral envelope with a host cell membrane or by forming membrane pores. Membrane fusion and pore formation critically depend on the engagement of host cell receptors and receptor choice is a key determinant of viral tropism. The multi-faceted interplay between viral and cellular factors during virus entry is a fascinating field of study, which can provide important insight into viral pathogenesis and define new targets for intervention. This book provides a comprehensive overview of this exciting field of research.
  • Water and the cell 2006
  • Part I. Cellular Biology -- Chapter 1. Analyzing Retinal Axon Guidance in Zebrafish / Fabienne E. Poulain, John A. Gaynes, Cornelia Stacher Hörndli, Mei-Yee Law, Chi-Bin Chien -- Chapter 2. Imaging Blood Vessels in the Zebrafish / Makoto Kamei, Sumio Isogai, Weijun Pan, Brant M. Weinstein -- Chapter 3. Medaka Haploid Embryonic Stem Cells / Yunhan Hong -- Part II. Developmental and Neural Biology -- Chapter 4. Neurogenesis / Prisca Chapouton, Leanne Godinho -- Chapter 5. Studying Peripheral Sympathetic Nervous System Development and Neuroblastoma in Zebrafish / Rodney A. Stewart, Jeong-Soo Lee, Martina Lachnit, A. Thomas Look, John P. Kanki, Paul D. Henion -- Chapter 6. Analysis of the Retina in the Zebrafish Model / Andrei Avanesov, Jarema Malicki -- Chapter 7. Photoreceptor Structure and Development: Analyses using GFP Transgenes / Brian D. Perkins, James M. Fadool -- Chapter 8. Physiological Recordings from Zebrafish Lateral-Line Hair Cells and Afferent Neurons / Josef G. Trapani, Teresa Nicolson -- Chapter 9. Zebrafish Kidney Development / Iain A. Drummond, Alan J. Davidson -- Chapter 10. Molecular Regulation of Pancreas Development in Zebrafish / Robin A. Kimmel, Dirk Meyer -- Chapter 11. Monitoring Sleep and Arousal in Zebrafish / Jason Rihel, David A. Prober, Alexander F. Schier -- Chapter 12. Use of Flatbed Transparency Scanners in Zebrafish Research: Versatile and Economical Adjuncts to Traditional Imaging Tools for the Danio rerio Laboratory / Charles A. Lessman, Michael R. Taylor, Wilda Orisme, Ethan A. Carver.
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