Books by Subject

all 748 titles

Cell Biology

  • 2008 Cold Spring Harb Lab Press
    arranged by Terri Grodzicker, David Stewart, Bruce Stillman.
  • 1972-
  • 2010 Springer
    Denson G. Fujikawa, editor.
  • 2007 Springer Protocols
    edited by Amanda S. Coutts.
  • 2013 Springer Protocols
    edited by Amanda S. Coutts.
  • 2010 Springer
    edited by Simon Yona, Martin Stacey.
    The adhesion GPCRs : gene repertoire, phylogeny, and evolution / Helgi B. Schith, Karl J.V. Nordstrom, and Robert Fredriksson -- Cadherins : developmental roles and future challenges / Caroline J. Formstone -- Latrophilin signalling in tissue polarity and morphogenesis / Tobias Langenhan and Andreas P. Russ -- GPS proteolytic cleavage of adhesion-GPCRs / Hsi-Hsien Lin ... [et al.] -- The latrophilins : "split-personality" receptors / John-Paul Silva and Yuri A. Ushkaryov -- Studies on the very large G protein-coupled-receptor : from initial discovery to determining its role in sensorineural deafness in higher animals / D. Randy McMillan and Perrin C. White -- Adhesion-GPCRs in the CNS / Natalie Strokes and Xianhua Piao -- GPR56 interacts with extracellular matrix and regulates cancer progression / Lei Xu -- Adhesion-GPCRs in tumorigenesis / Gabriela Aust -- Immunity and adhesion-GPCRs / Simon Yona, Hsi-Hsien Lin, and Martin Stacey -- CD97 in leukocyte trafficking / Jorg Hamann ... [et al.] -- The role of CD97 in regulating adaptive T-cell responses / Ian Spendlove and Ruhcha Sutavani -- F4/80 : the macrophage specific adhesion-GPCR and its role in immunoregulation / Hsi-Hsien Lin ... [et al.] -- Signal transduction mediated through adhesion-GPCRs / Norikazu Mizuno and Hiroshi Itoh -- Emerging roles of brain specific angiogenesis inhibitor 1 / Daeho Park and Kodi S. Ravichandran -- Adhesion-GPCRs in the male reproductive tract / Ben Davies and Christiane Kirchhoff.
    Also available: Print – 2010
  • 2011 Springer
    Yury A. Rovensky.
    Extracellular matrix -- Cytoskeleton -- Pseudopodia and adhesion structures -- Adhesive interactions of tissue cells with the extracellular matrix -- Cell migration -- Cell responses to chemical heterogeneity of substrata: adhesive islets or paths -- Topographic cell responses -- Intercellular adhesive interactions.
  • 2011 Springer
    Yves-Gérard Illouz, Aris Sterodimas, editors.
    Cellular and Molecular Aspects of Adipose Tissue -- Isolation of Stem Cells from Human Adipose Tissue: Technique, Problems, and Pearls -- Principles of Liposuction -- Clinical Applications of Liposuction -- Principles of Autologous Fat Transplantation -- Clinical Applications of Autologous Fat Transplantation -- Adipose Stem Cells: From Liposuction to Adipose Tissue Engineering -- Storage of Adipose Stem Cells -- Engineered Scaffolds and Matrices: Tailored Biomaterials for Adipose Stem Cell Engineering -- Fundamentals and Principles of Biomolecules in Adipose Stem Cell Engineering -- Gene Therapy Used for Adipose Stem Cell Engineering -- Stem Cells from Human Adipose Tissue: A New Tool for Pharmacological Studies and for Clinical Applications -- Role of Adipose Stem Cells Therapy in Obesity -- Stem Cell Based Cardioregeneration and Adipose Tissue -- Adipose Stem Cell Engineering: Characterization and Current Application in Neurological Tissue Repair -- Adipose Stem Cell Engineering: Clinical Applications in Plastic and Reconstructive Surgery -- Orthopedic Use of Adipose-Derived Stem Cells -- Adipose-Derived Stem Cells: Characterization and Application in Urology -- Adipose Stem Cell Engineering: Characterization and Current Application in Otolaryngology -- Adipose Stem Cell Technologies for Tissue Regeneration in Dentistry -- Adipose Stem Cells, Tissue Engineering, and Solid Organ Transplantation and Regeneration -- Industrial Approaches to Adipose Stem Cells Engineering -- Future Research in Adipose Stem Cell Engineering -- Conclusions and Future Directions.
  • 2013 Springer
    Mikhail G. Kolonin, editor.
    Preface: Obesity, Adipose Tissue and Cancer Progression -- Metabolic Perturbations Associated with Adipose Tissue Dysfunction and the Obesity-Cancer Link -- Increased Adiposity and Colorectal Cancer -- Adiposity and Diabetes in Breast and Prostate Cancer -- Increased Adipocity and Endometrial Cancer Risk -- Adipokines: Soluble Factors from Adipose Tissue Implicated in Cancer -- Animal Models to Study the Interplay between Cancer and Obesity -- Unraveling the local influence of tumor-surrounding adipose tissue on tumor progression: cellular and molecular actors involved -- Trafficking of Cells from Adipose Tissue to Tumor Microenvironment -- The Impact of Obesity Intervention on Cancer: Clinical Perspectives -- Index.
  • 2012 Springer
    Michael E. Symonds, editor.
    1. Adipocyte Precursors: Developmental Origins, Self-Renewal, and Plasticity / Christian Dani and Nathalie Billon -- 2. Adipocyte Differentiation / José María Moreno-Navarrete and José Manuel Fernández-Real -- 3. Brown Adipose Tissue / Martin Klingenspor and Tobias Fromme -- 4. White Adipose Tissue / Stephane Gesta and C. Ronald Kahn -- 5. Sex Differences in Body Fat Distribution / Alain Veilleux and André Tchernof -- 6. Macrophages and Inflammation / Elise Dalmas, Joan Tordjman, Michèle Guerre-Millo, and Karine Clément -- 7. Adipocyte Growth and Factors Influencing Adipocyte Life Cycle / Srujana Rayalam and Clifton A. Baile -- 8. The Evolution of Mammalian Adipose Tissue / Caroline M. Pond -- 9. Dietary Determinants of Fat Mass and Body Composition / María A. Zulet, María J. Moreno-Aliaga, and J. Alfredo Martínez -- 10. The Genetic Determinants of Common Obesity-Susceptibility / Ruth J.F. Loos -- 11. Early Origins of Obesity and Developmental Regulation of Adiposity / Shalini Ojha and Helen Budge.
  • 2008 Springer Protocols
    edited by Kaiping Yang.
  • 2011 Springer Protocols
    edited by Jeffrey M. Gimble, Bruce A. Bunnell.
    Isolation and culture of rhesus adipose-derived stem cells -- Isolation of human adipose-derived stem cells from lipoaspirates -- Isolation of murine adipose-derived stem cells -- In vitro adult rat adipose tissue-derived stromal cell isolation and differentiation -- In vitro adult canine adipose tissue-derived stromal cell growth characteristics -- Adipogenic differentiation of adult equine mesenchymal stromal cells -- Isolation and culture of porcine adipose tissue-derived somatic stem cells -- Automated isolation and processing of adipose-derived stem and regenerative cells -- Methods for the purification and characterization of human adipose-derived stem cells -- Characterization of human adipose-derived stem cells using flow cytometry -- Evaluation of cellular and humoral immune responses to allogeneic adipose-derived stem/stromal cells -- Adipose-derived stromal cells: Cytokine expression and immune cell contaminants -- Gel-based and gel-free proteomic technologies -- Adipogenic differentiation of adipose-derived stem cells -- Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells -- Neural differentiation of human adipose tissue-derived stem cells -- Osteogenic differentiation strategies for adipose-derived mesenchymal stem cells -- Generation of adipose stromal cell-derived hepatic cells -- Adipose stem cell differentiation into smooth muscle cells -- Endothelial and cardiac regeneration from adipose tissues -- Epithelial differentiation of human adipose-derived stem cells -- Screening for epigenetic target genes that enhance reprogramming using lentiviral-delivered shRNA -- Adipogenic differentiation of human adipose-derived stem cells on 3D silk scaffolds -- A first approach for the production of human adipose tissue-derived stromal cells for therapeutic use -- Viral transduction of adipose-derived stem cells -- Use of adipose-derived stem cells in high-throughput screening to identify modulators of lipogenesis -- Preservation protocols for human adipose tissue-derived adult stem cells -- Soft tissue reconstruction -- Methods for analyzing microRNA expression and function during osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells -- The generation and the manipulation of human multipotent adipose-derived stem cells -- Cell sheet technology for tissue engineering: The self-assembly approach using adipose-derived stromal cells -- Differentiation of adipose-derived stem cells for tendon repair -- Adipose-derived stem cells for skin regeneration -- Adipose-derived stem cells for periodontal tissue regeneration.
  • 2012 Springer
    Kursad Turksen, editor.
  • 2006 ScienceDirect
    edited by Irina Klimanskaya, Robert Lanza.
    Ectoderm -- Mesoderm -- Endoderm -- Extraembryonic and perinatal stem cells.
    Also available: Print – 2006
  • 2011 Springer
    Donald G. Phinney, editor.
    Basic Biology of Adult Stem Cells -- Molecular Mechanisms Regulating Adult Stem Cell Self-Renewal -- Maintenance of Adult Stem Cells: Role of the Stem Cell Niche -- The Emerging Role of microRNAs in Adult Stem Cells -- Expression and Function of Pluripotency Genes in Adult Stem Cells -- Adult Stem Cell Plasticity Revisited --Characterization of Adult Stem Cells -- Lineage Tracing of Tissue-Specific Stem Cells In Vivo -- Surrogate Measures of Adult Stem Cell Self-Renewal: The Neural Stem Cell Paradigm -- ABC Transporters, Aldehyde Dehydrogenase, and Adult Stem Cells -- Regulation of Life Span and Immortalization -- Regulation of Life Span in Adult Stem Cells -- The Cancer Stem Cell Paradigm.
  • 2015 Karger
    volume editor, Karl Lenhard Rudolph.
    Speakers at the symposium -- Stem cells in adult intestine / Feng, J., Sperka, T. -- Molecular mechanisms of muscle stem cell aging / Schwërer, S., Tümpel, S. -- Mechanism of functional alterations in hematopoietic stem cell aging / Morita, Y. -- The microenvironment, aging, and disease / Geiger, H. -- Sestrins in aging and metabolism / Tang, D., Tao S. -- Telomeres and stem cell aging / Felix, D.A. -- DNA damage and checkpoint responses in adult stem cells / Romanov, V., Shukla, A., Ju, Z.
  • 2010 CRCnetBASE
    edited by Andrei G. Pakhomov, Damijan Miklavčič, Marko S. Markov.
    "A reflection of the intense study of the effects of electromagnetic fields on living tissues that has taken place during the last several decades, this book discusses the theoretical and experimental evidence and considerations the effects of strong electromagnetic fields and/or electric pulses and their importance in medicine and biology. The authors present the basic techniques applied in electroporation and the advanced methods for creation of nanopores, highlighting their basic science and clinical applications. Topics include nano electroporation, classic electroporation, experimental evidence for electroporation of living cells, and electroporation for cancer and wound healing"--Provided by publisher.
  • v. 177-, 2005- Springer
  • 2012 Springer
    Roberto Scatena, Alvaro Mordente, Bruno Giardina, editors.
    1. Cancer Stem Cells: A Revisitation of the "Anaplasia" Concept / Roberto Scatena -- 2. Stem Cells and Cancer Stem Cells: New Insights / Toru Kondo -- 3. Molecular Biology of Cancer Stem Cells / Oswaldo Keith Okamoto -- 4. Biomarkers of Cancer Stem Cells / Jun Dou and Ning Gu -- 5. Cancer Stem Cells and the Microenvironment / Alfonso Colombatti, Carla Danussi, Eliana Pivetta, and Paola Spessotto -- 6. Leukemia Stem Cells / Steven W. Lane and David A. Williams -- 7. Cancer Stem Cells and the Central Nervous System / Serdar Korur, Maria Maddalena Lino, and Adrian Merlo -- 8. Cancer Stem Cells and Glioblastoma Multiforme: Pathophysiological and Clinical Aspects / Akio Soeda, Mark E. Shaffrey, and Deric M. Park -- 9. Breast Cancer Stem Cells / Nuria Rodríguez Salas, Enrique González González, and Carlos Gamallo Amat -- 10. Colon Cancer Stem Cells / Ugo Testa -- 11. Liver Tumor-Initiating Cells/Cancer Stem Cells: Past Studies, Current Status, and Future Perspectives / Kwan Ho Tang, Stephanie Ma, and Xin-Yuan Guan -- 12. Pancreatic Cancer Stem Cells / Erica N. Proctor and Diane M. Simeone -- 13. Cancer Stem Cells and Renal Carcinoma / Benedetta Bussolati and Giovanni Camussi -- 14. Cancer Stem Cells: Proteomic Approaches for New Potential Diagnostic and Prognostic Biomarkers / Patrizia Bottoni, Bruno Giardina, and Roberto Scatena -- 15. Cancer Stem Cells: An Innovative Therapeutic Approach / Roberto Scatena, Patrizia Bottoni, Alessandro Pontoglio, Salvatore Scarà, and Bruno Giardina -- 16. Cancer Stem Cell and ATP-Binding Cassette: Which Role in Chemoresistance? / Andrea Silvestrini, Elisabetta Meucci, Giuseppe Ettore Martorana, Bruno Giardina, and Alvaro Mordente -- 17. Stem Cells and Cancer Stem Cells: Biological and Clinical Interrelationships / Cinzia Bagalà and Carlo Barone -- 18. Immunomodulatory Functions of Cancer Stem Cells / Tobias Schatton, Jennifer Y. Lin, and Markus H. Frank.
  • 2012 Springer
    Roberto Scatena, Patrizia Bottoni, Bruno Giardina, editors.
    Also available: Print – 2012
  • 2011 Springer
    Niall M. Adams, Paul S. Freemont, editors.
  • 2012 Karger
    volume editor, Karl Lenhard Rudolph.
    Speakers at the symposium / Morita, M. -- Aging of the niche and the microenvironment and its role in stem cell aging / Geiger, H. -- Hematopoietic stem cell aging and cancer / Chen, Y., Ju, Z. -- DNA damage, checkpoint responses, and cell cycle control in aging stem cells / Kleinhans, K.N., Burkhalter, M.D. -- Hematopoietic stem cell aging and fate decision / Illing, A., Morita, Y. -- Stem cell therapy and stress response in pancreas and intestine -- Sperka, T., Omrani, O. -- Niche-stem cell interations and environmental influences / Tang, D. -- Stem cells and metabolism / Missios, P., Guachalla, L. -- Molecular mechanisms of muscle stem cell aging / Baig, A.H., Tümpel, S. -- Neural stem cells in development and aging / Schmidt-Strassburger, U.
  • 2012 Springer
    Hossein Baharvand, Nasser Aghdami, editors.
    Advances in stem cell research discusses recent advances in stem cell science, including therapeutic applications. This volume covers such topics as biomanufacturing iPS cells for therapeutic applications, techniques for controlling stem cell fate decisions, as well as current basic research in such areas as germ line stem cells, genomics and proteomics in stem cell research. It is a useful book for biology and clinical scientists, especially young investigators and stem cell biology students who are newly entering the world of stem cells research. The editors hope that the new knowledge and research outlined in this book will help contribute to new therapies for a wide variety of diseases that presently afflict humanity.
  • 2011 Springer
    David Wallach, Andrew Kovalenko, Marc Feldmann, editors.
    Roles of the TNF family in tissue homeostasis and normal development -- Novel aspects of signaling activation by the TNF family: novel aspects of the functions of the TRAFs and cIAPs -- Novel aspects of signaling activation by the TNF: ubiquitin modification of signaling proteins -- Novel aspects of signaling activation by the TNF family: TNF signaling and gene regulatory networks -- Roles of the TNF family in infectious diseases and interrelationship of the TNF family and pattern recognition receptor signaling: roles of the TNF family in infectious diseases -- Roles of the TNF family in infectious diseases and interrelationship of the TNF family and pattern recognition receptor signaling: interrelationship of the TNF family and pattern recognition receptor signaling -- Novel aspects of immune regulation by the TNF family -- Control of lymphocyte function and repertoire by the TNF family -- Advances in applying our knowledge to therapy: mechanisms of pathological and therapeutic effects of the TNF family -- Advances in applying our knowledge to therapy: rational design of new means for therapeutic modulation of function of the TNF family -- Roles of the TNF family in neuronal development, function, and pathology -- Cell death mechanisms controlled by the TNF family -- Contributors to abstracts of talks and posters at the 12th TNF Conference -- Abstracts of talks and posters presented at the 12th TNF Conference.
    Also available: Print – 2011
  • 2014 Karger
    volume editors, Ladislas Robert, Tamas Fülöp.
    The Commitment of human cells to senescence / R. Holliday -- Evolutionary theories of aging can explain why we age / E. Le Bourg -- Control of cell replication during aging / A. Macieira-Coelho -- Cell senescence : role in aging and age-related diseases / J. Campisi, L. Robert, -- Aging of cell membranes : facts and theories / I. Zs.-Nagy -- Oxidative stress, mitochondrial dysfunction, and the mitochondria theory of aging / Y. Kong, S.E. Trabucco, H. Zhang -- Aging of connective tissues : experimental facts and theoretical considerations / J. Labat-Robert, L. Robert -- Aging of cell communication : loss of receptor function / L. Robert, T. Fulop -- On the immunological theory of aging / T. Fulop, J.M. Witkowski, G. Pawelec, C. Alan, A. Larbi -- Aging of the brain, dementias, role of infectious proteins : facts and theories / F. Morinet -- Aging as alteration / P.-A. Miquel -- Longevity and its regulation : centenarians and beyond / L. Robert, T. Fulop.
  • 2012 Springer
    edited by Michael Breitenbach, S. Michal Jazwinski, Peter Laun.
    Introduction -- Oxidative stresses and ageing -- The role of mitochondria in the aging processes of yeast -- The retrograde response and other pathways of interorganelle communication in yeast replicative aging -- Chronological aging in Saccharomyces cerevisiae -- Aging and the survival of quiescent and non-quiescent cells in yeast stationary-phase cultures -- Maximising the yeast chronological lifespan -- Amino acid homeostasis and chronological longevity in Saccharomyces cerevisiae -- DNA damage and DNA replication stress in yeast models of aging -- Yeast aging and apoptosis -- Cellular homeostasis in fungi: impact on the aging process -- Genome-wide analysis of yeast aging -- Genetic approaches to aging in budding and fission yeasts: new connections and new opportunities -- Evolution of asymmetric damage segregaton: a modelling approach -- Cellular ageing and the actin cyoskeleton.
  • 2007
    Zachary S. Pincus.
    Status: Not Checked OutLane Catalog Record
  • 2007
    [edited by] Robert M. Klein George C. Enders.
    Status: Not Checked OutLane Catalog Record
  • 2010
    [edited by] Robert M. Klein, George C. Enders.
    Status: Not Checked OutLane Catalog Record
  • 2013 Springer
    Shaker A. Mousa, Paul J. Davis, editors.
    This book is a major update of novel targets in angiogenesis modulation, including pro- and anti-angiogenesis. There is in-depth coverage of preclinical and clinical methods and models, investigational status, and clinical applications. The impact of nanotechnology in advancing the applications of pro-and anti-angiogenesis strategies is also highlighted, along with stem cell and biotechnologies in research and development of angiogenesis modulating targets.
  • 2014 Springer Protocols
    edited by Ralf Pörtner.
    High-throughput synchronization of mammalian cell cultures by spiral microfluidics / Wong Cheng Lee, Ali Asgar S. Bhagat, and Chwee Teck Lim -- Insect cell line development using Flp-mediated cassette exchange technology / João Vidigal ... [et al.] -- Scalable transient gene expression in adherent mammalian cells using polyethylenimine / Lukas Fliedl and Christian Kaisermayer -- Scalable transient protein expression / Henry C. Chiou ... [et al.] -- DNA profiling and characterization of animal cell lines / Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins -- Tools for high-throughput process and medium optimization / Martin Jordan and Matthieu Stettler -- Designing media for animal cell culture : CHO cells, the industrial standard / Karlheinz Landauer -- Feed optimization in fed-batch culture / Ana Rita Costa ... [et al.] -- Screening and optimization of chemically defined media and feeds with integrated and statistical approaches / Zhihua Xiao ... [et al.] -- Evaluation of solid and porous microcarriers for cell growth and production of recombinant proteins / Maria Elisa Rodrigues ... [et al.] -- Microbioreactors and scale-down models : growth of CHO cells using the pall micro24 microreactor system / Steve R.C. Warr -- Monitoring cell growth, viability, and apoptosis / Michael Butler, Maureen Spearman, and Katrin Braasch -- Metabolic flux estimation in mammalian cell cultures / Chetan T. Goudar ... [et al.] -- Quenching methods for the analysis of intracellular metabolites / Judith Wahrheit and Elmar Heinzle -- NMR methods for metabolomics of mammalian cell culture bioreactors / Nelly Aranibar and Michael D. Reily -- ¹H-NMR protocol for exometabolome analysis of cultured mammalian cells / Tiago M. Duarte ... [et al.] -- Microfluidic perfusion culture / Koji Hattori, Shinji Sugiura, and Toshiyuki Kanamori -- Disposable bioreactors for inoculum production and protein expression / Regine Eibl, Christian Löffelholz, and Dieter Eibl -- Configuration of bioreactors / Dirk E. Martens, Evert J.van den End, and Mathieu Streefland -- Process analytical technology (PAT) in insect and mammalian cell culture processes : dielectric spectroscopy and focused beam reflectance measurement (FBRM) / Damir Druzinec ... [et a.] -- Cell assessment by at-line microscopy / Alexander Babitzky, Patrick Lindner, and Thomas Scheper -- Seed train optimization for cell culture / Björn Frahm -- Vaccine production : upstream processing with adherent or suspension cell lines / Yvonne Genzel ... [et al.] -- Efficient processes for protein expression using recombinant baculovirus particles / Heike Cremer ... [et al.] -- Chromatographic techniques in the downstream processing of proteins in biotechnology / Ruth Freitag -- Virus separation using membranes / Tanja A. Grein, Ronald Michalsky, and Peter Czermak.
  • 2015 Springer
    edited by Mohamed Al-Rubeai.
    Animal cells are the preferred "cell factories" for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a℗ℓrecommended volume for all biotechnology libraries.
  • 2009 Springer
    Koji Ikura ... [et al.], editors.
  • 2014 Springer Protocols
    edited by Bruno Christ, Jana Oerlecke, and Peggy Stock, Department of Surgery, University of Leipzig, Leipzig, Germany.
    Stem cells : are we ready for therapy? / Insa S. Schroeder -- Aurigon's point of view on the safety assessment of cell-based therapies : an experience based on the participation in 15 ATMPs projects / Emmanuelle Cornali -- Preconditioning of the liver for efficient repopulation by primary hepatocyte transplants / Petra Krause, Margret Rave-Frank, Hans Christiansen, and Sarah Koenig -- Age-dependent hepatocyte transplantation for functional liver tissue reconstitution / Peggy Stock -- Treatment of NASH with human mesenchymal stem cells in the immunodeficient mouse / Sandra Winkler and Bruno Christ -- In vivo evaluation of the therapeutic potential of human adipose tissue-derived mesenchymal stem cells for acute liver disease / Takeshi Katsuda, Hayato Kurata, Rie Tamai, Agnieszka Banas, Tsuyoshi Ishii, Shumpei Ishikawa, and Takahiro Ochiya -- A mouse model of liver injury to evaluate paracrine and endocrine effects of bone marrow mesenchymal stem cells / Chiung-Kuei Huang [and four others] -- Animal models to test hiPS-derived hepatocytes in the context of inherited metabolic liver diseases / Mathilde Dusséaux, Sylvie Darche, and Helene Strick-Marchand -- Support of hepatic regeneration by trophic factors from liver-derived mesenchymal stromal/stem cells / Suomi M.G. Fouraschen [and three others] -- Assessment of functional competence of endothelial cells from human pluripotent stem cells in zebrafish embryos / Valeria V. Orlova [and three others] -- Stem cell therapy for necrotizing enterocolitis : innovative techniques and procedures for pediatric translational research / Jixin Yang, Yanwei Su, and Gail E. Besner -- Exploring mesenchymal stem cell-derived extracellular vesicles in acute kidney injury / Stefania Bruno and Giovanni Camussi -- Angiogenic properties of mesenchymal stem cells in a mouse model of limb ischemia / Leonardo Martins, Priscila Keiko Matsumoto Martin, and Sang Won Han -- Methods to assess intestinal stem cell activity in response to microbes in Drosophila melanogaster / Philip L. Houtz and Nicolas Buchon -- Muscle pouch implantation : an ectopic bone formation model / Greg Asatrian, Le Chang, and Aaron W. James -- Bone defect repair in mice by mesenchymal stem cells / Sanjay Kumar -- Generation of osteoporosis in immune-compromised mice for stem cell therapy / Reeva Aggarwal, Vincent J. Pompili, and Hiranmoy Das -- Application of stem cells for the treatment of joint disease in horses / Walter Brehm, Janina Burk, and Uta Delling -- Adipogenic fate commitment of muscle-derived progenitor cells : isolation, culture, and differentiation / Anne-Marie Lau, Yu-Hua Tseng, and Tim J. Schulz -- Skeletal muscle stem cells for muscle regeneration / Johnny Kim and Thomas Braun -- Bone marrow stromal stem cells transplantation in mice with acute spinal cord injury / Virginie Neirinckx [and three others] -- Histological characterization and quantification of cellular events following neural and fibroblast( -like) stem cell grafting in healthy and demyelinated CNS tissue / Jelle Praet [and ten others] -- Improvement of neurological dysfunctions in aphakia mice, a model of Parkinson's disease, after transplantation of ES cell-derived dopaminergic neuronal precursors / Sangmi Chung, Jisook Moon, and Kwang-Soo Kim -- Methods for assessing the regenerative responses of neural tissue / Steven W. Poser, Maria Adele Rueger, and Andreas Androutsellis-Theotokis -- Mesenchymal stem cell-based therapy in a mouse model of experimental autoimmune encephalomyelitis (EAE) / Annie C. Bowles, Brittni A. Scruggs, and Bruce A. Bunnell -- Analysis of the neuroregenerative activities of mesenchymal stem cells in functional recovery after rat spinal cord injury / Akihito Yamamoto 0and three others] -- Therapeutic application of mesenchymal stromal cells in murine models of inflammatory bowel disease / Elena Gonzalez-Rey and Mario Delgado -- Mesenchymal stem cells attenuate rat graft-versus-host disease / Masayuki Fujino [and five others] -- Assessment of anti-donor T cell proliferation and cytotoxic T lymphocyte-mediated lympholysis in living donor kidney transplant patients / Aruna Rakha, Marta Todeschini, and Federica Casiraghi -- Modulation of autoimmune diseases by iPS cells / Fengyang Lei [and three others] -- A chimeric mouse model to study immunopathogenesis of HCV infection / Moses T. Bility, Anthony Curtis, and Lishan Su.
  • 2010 CRCnetBASE
    editor, Victor R. Preedy.
  • 2009 Springer
    edited by Gines Maria Salido and Juan Antonio Rosado.
  • 2009 Springer
    George G. Chen, Paul B.S. Lai, editors.
  • 2010 Springer
    Francesco Cecconi, Marcello D'Amelio, editors.
  • 2009 Springer
    Gáspár Bánfalvi.
  • 2012 Springer
    Colin A. Nurse, Constancio Gonzalez, Chris Peers, Nanduri R. Prabhakar, editors.
    Also available: Print – 2012
  • 2009 Springer
    [edited by] Constancio Gonzalez, Colin A. Nurse, Chris Peers.
    Also available: Print – 2009
  • 2009 Springer
    Vladimir Parpura, Philip G. Haydon, editors.
  • 2007 Springer
    Alvaro Macieira-Coelho (ed.).
  • 2014 Springer
    Henryk A. Domanski, editor.
    This book covers all of the diagnostic areas where FNAC is used today. This includes palpable lesions and lesions sampled using various radiological methods, and correlations with ancillary examinations detailed on an entity-by-entity basis. As well as being a complete atlas of the facts and findings important to FNAC, this atlas is a guide to diagnostic methods that optimize health care. The interaction of the cytologist or cytopathologist with other specialists (radiologists, oncologists and surgeons) involved in the diagnosis and treatment of patients with suspicious mass lesions is emphasized and illustrated throughout. ? With contributions from experts in the field internationally and abundant colour images Atlas of Fine Needle Aspiration Cytology provides a comprehensive and up-to-date guide to FNAC for pathologists, cytopathologists, radiologists, oncologists, surgeons and others involved in the diagnosis and treatment of patients with suspicious mass lesions.
  • 2012 Springer
    Michal Amit, Joseph Itskovitz-Eldor ; with contributions by Ilana Laevsky and Atara Novak.
  • 2012 Springer
    Terumasa Komuro.
    Part 1. Distribution and arrangement of ICC in the GI tract -- Stomach -- Small Intestine -- Duodenum -- Colon -- Caecum -- Ileocaecal Junction -- ICC Found in the Submucosal Layer -- Introduction -- Part 2. Ultrastructural demonstration of ICC and the allied cells -- Ultrastructural Demonstration of ICC -- Signal Pathways Between Nerves and Muscles Not Mediated by ICC -- Issues for Future Studies.
  • v. 1-, 2014 ScienceDirect
    v. 2, 2014 ScienceDirect
    v.3, 2014 ScienceDirect
    edited by M.A. Hayat.
    Volume 1. Molecular mechanisms -- Volume 2. Role in general diseases -- Volume 3. Mitophagy.
  • 2013 Springer
    Hong-Gang Wang, editor.
    This book covers the latest advances in the field of autophagy and cancer from the basics of the molecular machinery for autophagy induction and regulation up to current areas of interest, including modulation of autophagy and drug discovery for cancer prevention and treatment. The reader will be initially introduced to the historical context of autophagy, followed by a summary of the key players in autophagy, a discussion on the origin of autophagosomes, and an overview of signal transduction regulation of autophagy. The next chapters deal with the complex relationship between autophagy and tumorigenesis through in-depth discussions of how autophagy influences cell survival, apoptosis, inflammation, immunity, tumor microenvironment, metabolic stress, DNA damage, and genomic instability. The remaining chapters discuss how autophagy affects cancer cell response to therapy and how to best modulate autophagy for therapeutic benefit - what to target and how to target. We hope that this monograph will be an invaluable reference source for professionals, students, postdoctoral fellows, and senior scientists working in the fields of autophagy and cancer.
  • 2009 ScienceDirect
    edited by Daniel J. Klionsky.
    Also available: Print – 2009
  • 2009 Springer
    Beth Levine, Tamotsu Yoshimori, Vojo Deretic, editors.
    Also available: Print – 2009
  • 2009 ScienceDirect
    edited by Daniel J. Klionsky.
    Also available: Print – 2009
  • 2008 ScienceDirect
    edited by Daniel J. Klionsky.
    Also available: Print – 2008
  • 2016 Springer
    Tomohiro Kurosaki, Jürgen Wienands, editors.
    Assembly and function of the precursor B-cell receptor -- Receptor Dissociation and B cell activation -- Molecular mechanisms of B cell antigen gathering and endocytosis -- BTK signaling in B cell differentiation and autoimmunity -- The memory function of the B cell antigen receptor -- PI3K signaling in normal B cells and chronic lymphocytic leukemia (CLL) -- Role of Calcium Signaling in B Cell Activation and Biolog -- Roles of the NF-kappaB pathway in B-lymphocyte biology -- MAP kinase cascades in antigen receptor signaling and physiology.
  • 2015 Springer Protocols
    edited by Kumaran Narayanan, Monash University Malaysia, Bandar Sunway, Malaysia, Icahn School of Medicine at Mount Sinai, New York, USA.
  • 2013 Springer Protocols
    edited by Anne H. Delcour.
    Bird's eye view of the bacterial landscape / Natividad Ruiz -- Visualizing the bacterial cell surface : an overview / Harald Engelhardt -- Purification of the outer membrane usher protein and periplasmic chaperone-subunit complexes from the P and type 1 pilus systems / Nadine S. Henderson and David G. Thanassi -- Experimental manipulation of the microbial functional amyloid called curli / Yizhou Zhou [and others] -- Visualization of gram-positive bacterial pili / Chungyu Chang [and others] -- Single cell microfluidic studies of bacterial motility / Adrien Ducret, Olivier Theodoly, and Tam Mignot -- Chromatographic analysis of the Escherichia coli polysialic acid capsule / Susan M. Steenbergen and Eric R. Vimr -- Analysis of exopolysaccharides in Myxococcus xanthus using confocal laser scanning microscopy / Wei Hu, Renate Lux, and Wenyuan Shi -- Assessment of multidrug efflux assemblies by surface plasmon resonance / Elena B. Tikhonova and Helen I. Zgurskaya -- Fluorescence microscopy and proteomics to investigate subcellular localization, assembly, and function of the type II secretion system / Tanya L. Johnson [and others] -- Pore formation by T3SS translocators : liposome leakage assay / Eric Faudry, Caroline Perdu, and Ina Attree -- Isolation of bacterial type IV machine subassemblies / Mayukh K. Sarkar [and others] -- Production and crystallization of bacterial type V secretion proteins / Hye-Jeong Yeo -- Assembly of bacterial outer membrane proteins / Jan Grijpstra, Martine P. Bos, and Jan Tommassen -- Outer membrane of gram-negative bacteria : lipid A isolation and characterization / Jessica V. Hankins [and others] -- Quantitative and qualitative preparations of bacterial outer membrane vesicles / Halima Chutkan [and others] -- In vitro peptidoglycan synthesis assay with lipid II substrate / Jacob Biboy, Nhat Khai Bui, and Waldemar Vollmer -- Extraction of cell wall-bound teichoic acids and surface proteins from Listeria monocytogenes / Filipe Carvalho [and others] -- Subfractionation and analysis of the cell envelope (lipo) polysaccharides of Mycobacterium tuberculosis / Anna E. Grzegorzewicz and Mary Jackson -- Protein disulfide bond formation in the periplasm : determination / Katleen Denoncin [and others] -- Using reporter genes and the Escherichia coli ASKA overexpression library in screens for regulators of the gram negative envelope stress response / Julia L. Wong, Stefanie L. Vogt, and Tracy L. Raivio -- Isolation of bacteria envelope proteins / Shu Quan [and others] -- Patch clamp electrophysiology for the study of bacterial ion channels in giant spheroplasts of E. coli / Boris Martinac [and others] -- Electrophysiological characterization of bacterial pore-forming proteins in planar lipid bilayers / Owen S. Mapingire, Beau Wager, and Anne H. Delcour.
  • 2012 Springer Protocols
    edited by Cheryl D. Helgason, Cindy L. Miller.
    Detection of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- Eradication of mycoplasma contaminations / Cord C. Uphoff and Hans G. Drexler -- STR DNA typing of human cell lines : detection of intra- and interspecies cross-contamination / Wilhelm G. Dirks and Hans G. Drexler -- Classical and molecular cytogenetic analysis / Roderick A.F. MacLeod and Hans G. Drexler -- Fluorescent in situ hybridization of DNA probes in the interphase and metaphase stages of the cell cycle / Linda A. Cannizzaro -- Development of T lymphocytes in fetal thymus organ culture / Takeshi Nitta, Izumi Ohigashi, and Yousuke Takahama -- Generation, isolation, and engraftment of in vitro-derived human T cell progenitors / Génève Awong and Juan Carlos Zúñiga-Pflücker -- In vitro generation of human T regulatory cells : generation, culture, and analysis of FOXP3-transduced T cells / Alicia N. McMurchy and Megan K. Levings -- Simultaneous cloning and selection of hybridomas and transfected cell lines in semisolid media / Bert Wognum and Tracy Lee -- Isolation and characterization of mouse side population cells / Aysegul V. Ergen [and others] -- Stem cell identification by DyeCycle violet side population analysis / William G. Telford -- Isolation and characterization of cancer stem cells in vitro / Craig Gedye and Laurie Ailles -- Ex vivo differentiation of cord blood stem cells into megakaryocytes and platelets / Nicolas Pineault [and others] -- Generation and characterization of murine alternatively activated macrophages / Shelley B. Weisser [and others] -- Human long-term culture initiating cell assay / Min Liu, Cindy L. Miller, and Connie J. Eaves -- Long-term culture-initiating cell assay for mouse cells / Stefan Woehrer, Cindy L. Miller, and Connie J. Eaves -- Colony forming cell assays for human hematopoietic progenitor cells / Bert Wognum [and others] -- Studying leukocyte recruitment under flow conditions / Sean A. Parsons [and others] -- Generation and establishment of murine adherent cell lines / Rouzanna Istvanffy and Robert A.J. Oostendorp -- Isolation, enumeration, and expansion of human mesenchymal stem cells in culture / Ravenska Wagey and Brenton Short -- Isolation and culture of mesenchymal stem cells from mouse compact bone / Brenton Short and Ravenska Wagey -- Generation of a pool of human platelet lysate and efficient use in cell culture / Katharina Schallmoser and Dirk Strunk -- In vitro methods to culture primary human breast epithelial cells / Afshin Raouf and Yu Jia Sun -- Human prostate epithelial cell cultures / Johng S. Rhim -- Enzymatic dissociation, flow cytometric analysis, and culture of normal mouse mammary tissue / Michael Prater [and others] -- Isolation and characterization of human hair follicle epithelial cells / Keita Inoue and Kotaro Yoshimura -- Cocultivation of human oral keratinocytes and human osteoblast-like cells / Ricarda Glaum and Margit Wiedmann-Al-Ahmad -- Isolation and culture of skeletal muscle myofibers as a means to analyze satellite cells / Paul Keire [and others] -- Hepatic differentiation of embryonic stem cells by murine fetal liver mesenchymal cells / Takamichi Ishii, Kentaro Yasuchika, and Iwao Ikai -- Methods to culture, differentiate, and characterize neural stem cells from the adult and embryonic mouse central nervous system / Sharon A. Louis, Carmen K.H. Mak, and Brent A. Reynolds -- Feeder-independent culture systems for human pluripotent stem cells / Jennifer Moody -- Formation of embryoid bodies from human pluripotent stem cells using AggreWell plates / Jennifer Antonchuk -- Techniques in embryoid body formation from human pluripotent stem cells / Nirupama K. Shevde and Amber A. Mael.
  • 2011 Springer
    [edited by] Bernhelm Booss-Bavnbek, Beate Klösgen, Jesper Larsen, Flemming Pociot, Erik Renström.
    pt. 1. Systems biology approach to [beta]-cells -- pt. 2. Imaging and sensors -- pt. 3. Genetics and proteomics -- pt. 4. Physiological, pharmaceutical and clinical applications and perspectives -- pt. 5. Mathematical modelling and numerical simulation.
  • 2015 Springer
    Ritu Nayar, David C. Wilbur, editors.
    This book offers clear, up-to-date guidance on how to report cytologic findings in cervical, vaginal and anal samples in accordance with the 2014 Bethesda System Update. The new edition has been expanded and revised to take into account the advances and experience of the past decade. A new chapter has been added, the terminology and text have been updated, and various terminological and morphologic questions have been clarified. In addition, new images are included that reflect the experience gained with liquid-based cytology since the publication of the last edition in 2004. Among more than 300 images, some represent classic examples of an entity while others illustrate interpretative dilemmas, borderline cytomorphologic features or mimics of epithelial abnormalities. The Bethesda System for Reporting Cervical Cytology, with its user-friendly format, is a must have for pathologists, cytopathologists, pathology residents, cytotechnologists, and clinicians.
  • 2015 Springer
    Feridoun Karimi-Busheri, editor.
    1. Integration, networking, and global biobanking in the age of new biology -- 2. The future of biobanking: a conceptual look at how biobanks can respond to the growing human biospecimen needs of researchers -- 3. Sustainability of biobanks in the future -- 4. Biobanking: the future of cell preservation strategies -- 5. Biobanking for personalized medicine -- 6. A global view of breast tissue banking -- 7. Biobanking of cerebrospinal fluid for biomarker analysis in neurological diseases -- 8. Biobanking in the twenty-first century: driving population metrics into biobanking quality -- 9. Challenges in developing a cancer oriented-biobank: experience from a 17 year-old cancer biobank in Sao Paulo, Brazil -- 10. China biobanking -- 11. Establishing an iso-compliant modern cancer-biobank in a developing country: a model for international cooperation -- 12. Nursing and biobanking -- 13. A data-centric strategy for modern biobanking -- 14. The importance of quality patient advocacy to biobanks: a lay perspective from Independent Cancer Patients Voice (ICPV), based in United Kingdom -- Index.
    Also available: Print – 2015
  • 2012
    Oscar John Abilez.
    Cardiovascular disease affects more than 70 million Americans and is the number one cause of mortality in the United States. Because the regenerative capacity of cardiac tissue is limited, human pluripotent stem cells (hPSC) have emerged as a potential source for cellular-based therapies. However, for these therapies to be effective, sufficient amounts of differentiated cells must be produced, these cells must be identified and sorted, and, upon implantation, arrhythmias must be avoided. In this dissertation, I describe the biochemical control of hPSC for their directed differentiation into cardiomyocytes, electrical control for electrophysiology-based cell sorting, and optogenetic control for temporal synchronization. For future therapy, the in vivo application of optical stimulation could allow immediate, precise, and specific synchronization of efficiently derived and purified hPSC-CM with patient cardiac rates and rhythms. This, in turn, would significantly reduce the chance of arrhythmias arising from implanted hPSC-CM, and, therefore, contribute towards establishing a safe and effective cell-based therapy.
  • 2015 Springer
    Abhijit Chakrabarti.
    Consists of critical reviews and original research papers from the 2014 International Symposium on the "Biochemical Role of Eukaryotic Cell Surface Macromolecules". Topics covered include: · neurochemical and biochemical analysis of cell surface glycoconjugates · membrane skeletal organization · GPCRs and other receptors · biophysical approaches to study membrane interactions · glycoconjugate metabolism · dysregulation · molecular mechanisms involved in cell-cell and cell-matrix interaction · glycans in infectious and neurological diseases · cancer and glycosyltransferases as drug targets.
    Also available: Print – 2015
  • 2012 Springer
    Perumana R. Sudhakaran, Avadhesha Surolia, editors.
    Glycomics: An Overview of the Complex Glycocode / Garima Gupta and Avadhesha Surolia -- Structural Glycomic Approaches to Molecular Recognition Events on Cell Surfaces / Koichi Kato -- ISCSM2011 Chondroitin Sulfate E-type Structure at Tumor Cell Surface Is Involved in Experimental Metastasis / Kazuyuki Sugahara and Shuji Mizumoto -- Chondroitin Sulfate-Specific Novel Hydrolase in Human / Shuhei Yamada -- Identification of Endothelial Cell Surface Carbohydrate-Binding Receptors by Carbohydrate Ligand Mimicry Peptides / Michiko N. Fukuda -- Lysosomal Enzyme Sorting Receptors - Where Did They First Appear in the Animal Kingdom? / Suryanarayanaraju Vegiraju, Suresh Koduru and Siva Kumar Nadimpalli -- Endoplasmic Reticulum-Targeted Bcl-2 Inhibitable Mitochondrial Fragmentation Initiates ER Stress-Induced Cell Death / B. C. Bhavya, Deepa Indira, Mahendra Seervi, Jeena Joseph and Praveen K. Sobhan, et al. -- Interactions Between Caveolin-1 and Sphingolipids, and Their Functional Relevance / Sandro Sonnino, Simona Prioni, Vanna Chigorno and Alessandro Prinetti -- Cell Membrane Repair Pathway Involves Sensing of Dynamics of Caveolae and Caspase-1 / Saumya S. Srivastava and M. V. Krishnasastry -- Angiogenic Response of Endothelial Cells to Fibronectin / V. B. S. Kumar, R. I. Viji, M. S. Kiran and Perumana R. Sudhakaran -- Lactosylceramide Synthase as a Therapeutic Target to Mitigate Multiple Human Diseases in Animal Models / Subroto Chatterjee and Nezar Alsaeedi -- Advanced FRET Methodologies: Protein-Lipid Selectivity Detection and Quantification / Fábio Fernandes, Manuel Prieto and Luís M. S. Loura -- Mechanism of GPCR-Directed Autoantibodies in Diseases / Hamiyet Unal, Rajaganapathi Jagannathan and Sadashiva S. Karnik -- Role of Membrane Cholesterol in Leishmanial Infection / Amitabha Chattopadhyay and Md. Jafurulla -- How Intact Is the Basement Membrane? Role of MMPs / S. Asha Nair, Sankar Jagadeeshan, Ramachandran Indu, Perumana R. Sudhakaran and M. R. Pillai -- Apoptosis of Breast Cancer Cells: Modulation of Genes for Glycoconjugate Biosynthesis and Targeted Drug Delivery / Subhash Basu, Rui Ma, Joseph R. Moskal, Manju Basu and Sipra Banerjee -- Altered Expression of Sialidases in Human Cancer / Taeko Miyagi, K. Takahashi, S. Moriya, K. Hata and K. Yamamoto, et al. -- Poly-ADP-Ribosylation of Vascular Endothelial Growth Factor and Its Implications on Angiogenesis / S. Binu, S. J. Soumya, V. B. S. Kumar and Perumana R. Sudhakaran -- Sphingolipid-Binding Domain in the Serotonin1A Receptor / Amitabha Chattopadhyay, Yamuna Devi Paila, Sandeep Shrivastava, Shrish Tiwari and Pushpendra Singh, et al. -- Withanolide D, Carrying the Baton of Indian Rasayana Herb as a Lead Candidate of Antileukemic Agent in Modern Medicine / Susmita Mondal, Saptarshi Roy, Rita Maity, Asish Mallick and Rajender Sangwan, et al. -- Engineered Glucose to Generate a Spectroscopic Probe for Studying Carbohydrate Biology / Ashish Tripathi, Vibha Singh, K. G. Aishwarya, Gopala Krishna Aradhyam and Srinivas Hotha -- Changes in Sialic Acid Content of Jelly Coat in Pesticide-Exposed Frog Eggs and Their Influence on Fertilization / H. P. Gurushankara, S. V. Krishnamurthy and V. Vasudev.
    Also available: Print – 2012
  • 2013 Cambridge
    Insoo Hyun, Case Western Reserve University.
    "This book provides a sophisticated yet accessible account of emerging trends in stem cell research and their accompanying ethical issues"-- Provided by publisher.
  • 2010 Springer
    Livio Luzi ; in collaborazione con Roberto Codella ... [et al.].
  • 2006 Springer
    A.M. Smith, J.A. Callow (eds.).
  • 2013 Springer Protocols
    edited by Trygve O. Tollefsbol.
    Cell Senescence Culturing Methods / Huaping Chen, Yuanyuan Li, and Trygve O. Tollefsbol -- Digital Image Analysis of Cells Stained with the Senescence-Associated b-Galactosidase Assay / Liran I. Shlush and Sara Selig -- Analysis of Biomarkers of Caloric Restriction in Aging Cells / Yuanyuan Li and Trygve O. Tollefsbol -- Cell Sorting of Young and Senescent Cells / Graeme Hewitt, Thomas von Zglinicki, and Joã̃o F. Passos -- Studying the Replicative Life Span of Yeast Cells / David A. Sinclair -- Methods for Creating Mutations in C. elegans That Extend Lifespan / Dayong Wang, Min Cao, Jessica Dinh, and Yuqing Dong -- Aging Studies in Drosophila Melanogaster / Yaning Sun, Jason Yolitz, Cecilia Wang, Edward Spangler, Ming Zhan, and Sige Zou -- The Use of Calorie Restriction Mimetics to Study Aging / Munehiro Kitada and Daisuke Koya -- Using Somatic-Cell Nuclear Transfer to Study Aging / Satoshi Kishigami, Ah Reum Lee, and Teruhiko Wakayama -- Induction of Cellular Senescence by Oncogenic RAS / Romi Gupta and Narendra Wajapeyee -- Methods of Cellular Senescence Induction Using Oxidative Stress / Zhe Wang, Dandan Wei, and Hengyi Xiao -- Methods of Testing Pharmacological Drugs Effects on Aging and Life Span in Mice / Vadimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski -- Mitochondria-Targeted Antiaging Gene Therapy with Adeno-associated Viral Vectors / Dejia Li and Dongsheng Duan -- Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration / Nadav Bleich Kimelman, Ilan Kallai, Dmitriy Sheyn, Wafa Tawackoli, Zulma Gazit, Gadi Pelled, and Dan Gazit -- Exometabolomic Mapping of Caenorhabditis elegans: A Tool to Noninvasively Investigate Aging / Robert J. Mishur, Jeffrey A. Butler, and Shane L. Rea -- Detecting Polymorphisms in Human Longevity Studies: HLA Typing and SNP Genotyping by Amplicon Sequencing / Gilberto Vargas-Alarcón and Carmina Flores-Domı́nguez -- Gel Electrophoresis-Based Proteomics of Senescent Tissues / Steven Carberry and Kay Ohlendieck -- Single-Cell Semiconductor Sequencing / Andrea B. Kohn, Tatiana P. Moroz, Jeffrey P. Barnes, Mandy Netherton, and Leonid L. Moroz -- Application of DNA Microarray Technology to Gerontological Studies / Kiyoshi Masuda, Yuki Kuwano, Kensei Nishida, and Kazuhito Rokutan -- Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells / Carmen M. Koch and Wolfgang Wagner -- Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging / Leonid L. Moroz and Andrea B. Kohn
  • 2007 Springer Protocols
    edited by Trygve O. Tollefsbol.
  • 2015 Springer
    Vasilis Vasiliou, Samir Zakhari, Helmut K. Seitz, Jan B. Hoek, editors.
    In recent years, a significant amount of research has emerged connecting the link between alcohol and cancer. The field has rapidly advanced, especially since the complex connection between alcohol and cancer has several unique sub areas that are being investigated and this volume gives a comprehensive overview of these advancements.
    Also available: Print – 2015
  • 2015 Thieme-Connect
    [edited by] James P. Stannard, James L. Cook, Lisa A. Fortier.
    US definitions, current use, and FDA stance on use of platelet-rich plasma in sports medicine / Knut Beitzel, Donald Allen, John Apostolakos, Ryan P. Russell, Mary Beth McCarthy, Gregory J. Gallo, Mark P. Cote, and Augustus D. Mazzocca -- European definitions, current use, and EMA stance of platelet-rich plasma in sports medicine / Stefano Fiorentino, Alice Roffi, Giuseppe Filardo, Maurilio Marcacci, and Elizaveta Kon -- FDA regulation of adult stem cell therapies as used in sports medicine / Mary Ann Chirba, Berkley Sweetapple, Charles P. Hannon, and John A. Anderson -- Cellular chondroplasty : a new technology for joint regeneration / Mary Murphy and Frank Barry -- Autologous-conditioned serum : evidence for use in the knee / David D. Frisbie -- Role of platelet-rich plasma in articular cartilage injury and disease / Randy Mascarenhas, Bryan M. Saltzman, Lisa A. Fortier, and Brian J. Cole -- Use of platelet-rich plasma for patellar tendon and medial collateral ligament injuries : best current clinical practice / Isabel Andia and Nicola Maffulli -- Can platelet-rich plasma enhance anterior cruciate ligament and meniscal repair? / Ian D. Hutchinson, Scott A. Rodeo, Gabriel S. Perrone, Martha M. Murray -- How should we evaluate outcomes for use of biologics in the knee? / Christopher M. LaPrade, Evan W. James, Robert F. LaPrade, Lars Engebretsen -- Biologics used in bone healing / Brett D. Crist and Gregory J. Della Rocca -- Clinical decision making for use of biologics in orthopaedic practice / James L. Cook and James P. Stannard.
  • 2009 Springer
    Dov Zipori.
  • 2012 Springer
    Tamás Rőszer.
    This book fills in a gap in the NO literature. Recent progress in the field of NO-biology shows that NO is generated within distinct cell compartments, including specific plasma membrane regions, mitochondria, chloroplasts, peroxisomes, the Golgi-complex and intracellular membrane systems. NO synthesis plays specific roles in these compartments and, in turn, cell organelles also control intracellular NO levels. This monograph focuses on the roles played by the subcellular NO-signaling microdomains in the prokaryote-, fungus-, plant- and animal cells and shows how NO behaves as an intracellular signal in distinct cellular environments. This monograph also provides a summary of our knowledge on how NO synthesis came through evolution to be associated with organelles and subcellular compartments. Promotes the novel ideas that some functions of NO and its associations with subcellular units have been conserved during the evolution of the cell. A special chapter is dedicated to the biomedical relevance of subcellular NO synthesis, and this chapter also discusses the evidence that altered compartmentalization of NO-producing enzymes causes disease.
  • 2014 Cambridge
    2014 Knovel
    edited by Peter X. Ma, University of Michigan.
    "Written by world-leading experts, this book focusses on the role of biomaterials in stem cell research and regenerative medicine. Emphasising basic principles and methodology, it covers stem cell interactions, fabrication technologies, design principles, physical characterisation and biological evaluation, across a broad variety of systems and biomaterials. Topics include: stem cell biology, including embryonic stem cells, IPS, HSC and progenitor cells; modern scaffold structures, including biopolymer, bioceramic, micro- and nanofiber, ECM and biohydrogel; advanced fabrication technologies, including computer-aided tissue engineering and organ printing; cutting-edge drug delivery systems and gene therapy techniques; medical applications spanning hard and soft tissues, the cardiovascular system and organ regeneration. With a contribution by Nobel laureate Shinya Yamanaka, this is a must-have reference for anyone in the field of biomaterials, stem cell biology and engineering, tissue engineering and regenerative medicine"--Provided by publisher.
  • 2014 Springer Protocols
    edited by Gordana Vunjak-Novakovic, Department of Biomedical Engineering and Department of Medicine, Columbia University, New York, NY, USA, Kursad Turksen, Sprott Centre for Stem Cell Research, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
    Derivation and network formation of vascular cells from human pluripotent stem cells -- High-throughput cell aggregate culture for stem cell chondrogenesis -- Microfluidic device to culture 3D in vitro human capillary networks -- Multifunction co-culture model for evaluating cell-cell interactions -- Multiwell plate tools for controlling cellular alignment with grooved topography -- Bioreactor cultivation of anatomically shaped human bone grafts -- Determining the role of matrix compliance in the differentiation of mammary stem cells -- Conjugation of proteins to polymer chains to create multivalent molecules -- An assay to quantify chemotactic properties of degradation products from extracellular matrix -- Biomimetic strategies incorporating enzymes into CaP coatings mimicking the in vivo environment -- Fabrication of biofunctionalized, cell-laden macroporous 3D PEG hydrogels as bone marrow analogs for the cultivation of human hematopoietic stem and progenitor cells -- Extracellular matrix mimetic peptide scaffolds for neural stem cell culture and differentiation -- The delivery and evaluation of RNAi therapeutics for heterotopic ossification pathologies -- Mimicking bone microenvironment for directing adipose tissue-derived mesenchymal stem cells into osteogenic differentiation -- Cultivation of human bone-like tissue from pluripotent stem cell-derived osteogenic progenitors in perfusion bioreactors.
  • 2015 ScienceDirect
    edited by Ewa K. Paluch.
  • 2008 ScienceDirect
    edited by John J. Correia, H. William Detrich, III.
    Also available: Print – 2008
  • 2008 ScienceDirect
    edited by John Correia and H. William Detrich, III.
    Also available: Print – 2008
  • 2010 Springer
    Michael P. Cancro, editor.
  • 2013 Cambridge
    edited by Reinhold Munker, Gerhard C. Hildebrandt, Hillard M Lazarus, Kerry Atkinson.
    Machine generated contents note: Preface; Part I. Basic Science: 1. Hematopoietic cell transplantation: past, present and future Reinhold Munker; 2. Basic science Reinhold Munker and Kerry Atkinson; Part II. Hematologic Malignancies: 3. Therapeutic decision making in BMT/SCT for acute myeloid leukemia Reinhold Munker, Gerhard C. Hildebrandt and Kerry Atkinson; 4. Therapeutic decision making in BMT/SCT for acute lymphoblastic leukemia Reinhold Munker, Vishwas Sakhalkar, Hillard M. Lazarus and Kerry Atkinson; 5. Therapeutic decision making in BMT/SCT for chronic myeloid leukemia and other myeloproliferative syndromes Reinhold Munker, Hillard M. Lazarus and Kerry Atkinson; 6. Therapeutic decision making in BMT/SCT for chronic lymphatic leukemia Nebu Verghese Koshy, Reinhold Munker, Hillard M. Lazarus and Kerry Atkinson; 7. Therapeutic decision making in BMT/SCT for myelodysplasia Carolina Escobar, Reinhold Munker and Kerry Atkinson; 8. Hematopoietic cell transplantation for non-Hodgkin lymphoma Saurabh Chhabra and Ginna G. Laport; 9. Therapeutic decision making in BMT/SCT for Hodgkin lymphoma Reinhold Munker, Hillard M. Lazarus and Kerry Atkinson; 10. Therapeutic decision making in hematopoietic stem cell transplantation for multiple myeloma Reinhold Munker, Oscar F. Ballester, Hillard M. Lazarus and Kerry Atkinson; 11. Therapeutic decision making in SCT for amyloidosis Reinhold Munker, Hillard M. Lazarus and Kerry Atkinson; Part III. Solid Tumors: 12. Therapeutic decision making in BMT/SCT for nonseminomatous germ cell tumor of testis (NSGCT) Reinhold Munker, Hillard M. Lazarus and Kerry Atkinson; 13. Therapeutic decision making in BMT/SCT for renal cell cancer Richard Childs and Reinhold Munker; 14. Therapeutic decision making in BMT/SCT for soft tissue sarcomas Reinhold Munker, Vishwas Sakhalkar, Hillard M. Lazarus and Kerry Atkinson; Part IV. Nonmalignant Disorders: 15. Therapeutic decision making in BMT/SCT for severe aplastic anemia Reinhold Munker, Anna Locasciulli and Kerry Atkinson; 16. Therapeutic decision making in BMT/SCT for congenital immunodeficiencies Vishwas Sakhalkar, Reinhold Munker and Kerry Atkinson; 17. Therapeutic decision making in BMT/SCT for hemoglobinopathies Shalini Shenoy, Reinhold Munker and Kerry Atkinson; 18. Hematopoietic stem cell transplantation for inborn errors of metabolism and neurodegenerative disorders Robert F. Wynn, Jaap J. Boelens and Muhammad A. Saif; 19. Therapeutic decision making in BMT/SCT for autoimmune disorders Reinhold Munker; 20. Cellular therapy Hans-Jochem Kolb, Kerry Atkinson and Reinhold Munker; Part V. Practical Aspects and Procedures: 21. Practical aspects and procedures, including conditioning protocols and haploidentical transplantation Reinhold Munker, Hillard M. Lazarus and Kerry Atkinson; 22. Umbilical cord blood as alternative allogeneic graft source: clinical banking and transplant outcomes Mary J. Laughlin and Reinhold Munker; Part VI. Complications: 23. Pathobiology of graft-versus-host disease Pavan Reddy; 24. Diagnosis and treatment of graft-versus-host-disease Daniel R. Couriel; 25. Management and prophylaxis of infections after BMT/SCT Nicholas Barber and Alison G. Freifeld; 26. Organ-related and miscellaneous complications Gerhard C. Hildebrandt, Reinhold Munker, Ullrich Duffner, Daniel Wolff, Michael Stadler, Tina Dietrich, Klemens Angstwurm, Amanda Sun, Binu Nair, Hillard M. Lazarus and Kerry Atkinson; Part VII. The BMT/SCT Pharmacopoeia: 27. The BMT/SCT pharmacopoeia Jill Comeau, Reinhold Munker and Kerry Atkinson; Part VIII. HLA-Testing and Laboratory Medicine: 28. HLA-testing and laboratory medicine Nicholas R. DiPaola, Reinhold Munker and Kerry Atkinson; Appendix: guide to the internet and literature databases relevant for bone marrow and stem cell transplantation; Index.
  • 2013 Wiley
    Jianqing Wang, Qiong Wang.
    Introduction to Body Area Communications -- Electromagnetic Characteristics of the Human Body -- Electromagnetic Analysis Methods -- Body Area Channel Modeling -- Modulation/Demodulation -- Body Area Communication Performance -- Electromagnetic Compatibility Considerations -- Summary and Future Challenges.
  • 2014 Springer Protocols
    edited by Meral Beksac.
    Reporter Gene Technologies for Imaging Cell Fates in Hematopoiesis / Sophie Kusy and Christopher H. Contag -- Flow Cytometry for Hematopoietic Cells / Daniela S. Krause, Michelle E. DeLelys, and Frederic I. Preffer -- Isolation and Characterization of Mesenchymal Stem Cells / Sedat Odabas, A. Eser Elçin, and Y. Murat Elçin -- Methods for Functional Analysis of Stem Cells / Michelle Escobedo-Cousin, J. Alejandro Madrigal, and Aurore Saudemont -- An Overview of HLA Typing for Heamatopoietic Stem Cell Transplantation / Katy Latham, Ann-Margaret Little, and J. Alejandro Madrigal -- HLA Typing with Sequence-Specific Oligonucleotide Primed PCR (PCR-SSO) and Use of the Luminex Technology / Klara Dalva and Meral Beksaç -- Sequence Based Typing of HLA: An Improved Group Specific Full Length Gene Sequencing Approach / Christina E.M. Voorter, Fausto Palusci, and Marcel G.J. Tilanus -- Molecular Typing Methods for Minor Histocompatibility Antigens / Eric Spierings -- Natural Killer Cells and Killer-cell Immunoglobulin-like Receptor Polymorphisms: Their Role in Hematopoietic Stem Cell Transplantation / Jennifer Schellekens, Katia Gagne, and Steven G.E. Marsh -- Methods of Detection of Immune Reconstitution and T Regulatory Cells by Flow Cytometry / Richard Charles Duggleby and J. Alejandro Madrigal -- Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell Transplantation in Myeloid Disorders / Ahmet H. Elmaagacli -- Molecular Methods for Detection of Minimal Residual Disease Following Transplantation in Lymphoid and Plasma Cell Disorders / Paolo Corradini and Cristiana Carniti -- Molecular Methods for Detection of Invasive Fungal Infections and Mycobacteria and Their Clinical Significance in Hematopoietic Stem Cell Transplantation / Pinar Yurdakul and Sule Colakoglu -- Post-transplant Monitoring of Chimerism by Lineage-specific Analysis / Sandra Preuner and Thomas Lion -- Urinary Proteomics Employing Capillary Electrophoresis Coupled to Mass Spectrometry in The Monitoring Of Patients After Stem Cell Transplantation / Eva M. Weissinger, William Mullen, and Amaya Albalat.
  • 2012 Springer Protocols
    edited by Miep H. Helfrich, Stuart H. Ralston.
    Primary human osteoblast cultures / Jane P. Dillon [and others] -- Osteoblast isolation from murine calvaria and long bones / Astrid D. Bakker and Jenneke Klein-Nulend -- Rat osteoblast cultures / Isabel R. Orriss, Sarah E.B. Taylor, and Timothy R. Arnett -- Isolation of primary avian osteocytes / Cor M. Semeins, Astrid D. Bakker, and Jenneke Klein-Nulend -- Isolation of mouse osteocytes using cell fractionation for gene expression analysis/ Christine Halleux [and others] -- Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5 / Jennifer Rosser and Lynda F. Bonewald -- Isolation, differentiation, and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo / Rahul S. Tare [and others] -- Rodent osteoclast cultures / Isabel R. Orriss and Timothy R. Arnett -- Isolation and culture of primary chicken osteoclasts / Patricia Collin-Osdoby and Philip Osdoby -- Isolation and purification of rabbit osteoclasts / Fraser P. Coxon, Michael J. Rogers, and Julie C. Crockett -- Generation of human osteoclasts from peripheral blood / Kim Henriksen [and others] -- Osteoclast formation in mouse co-cultures / Cecile Itzstein and Robert J. Van 't Hof -- RANKL-mediated osteoclast formation from murine RAW 264.7 cells / Patricia Collin-Osdoby and Philip Osdoby -- Transfection of osteoclasts and osteoclast precursors / Julie C. Crockett, David J. Mellis, and Adam Taylor -- Analysis of signalling pathways by western blotting and immunoprecipitation / Aymen I. Idris -- Analysis of transcriptional regulation in bone cells / Huilin Jin and Stuart H. Ralston -- Extraction of nucleic acids from bone / Alun Hughes, Tracy L. Stewart, and Val Mann -- Analysis of gene expression in bone by quantitative RT/PCR / Alun Hughes -- Histomorphometry in rodents / Reinhold G. Erben and Martin Glösmann -- Studying gene expression in bone by in situ hybridization / Ina Kramer [and others] -- Immunostaining of skeletal tissues / Tobias B. Kurth and Cosimo De Bari -- Techniques for the study of apoptosis in bone / Sudeh Riahi and Brendon Noble -- Transmission electron microscopy of bone / Vincent Everts [and others] -- Scanning electron microscopy of bone / Alan Boyde -- Fluorescence imaging of osteoclasts using confocal microscopy / Fraser P. Coxon -- Live imaging of bone cell and organ cultures / Sarah L. Dallas and Patricia A. Veno -- Analysis of bone architecture in rodents using microcomputed tomography / Robert J. van 't Hof -- Bone measurements by peripheral quantitative computed tomography in rodents / Jürg A. Gasser and Johannes Willnecker -- Quantitative X-ray imaging of rodent bone by Faxitron / J.H. Duncan Bassett [and others] -- Bioluminescence imaging of bone metastasis in rodents / Thomas J.A. Snoeks [and others] -- Fourier transform infrared imaging of bone / Eleftherios P. Paschalis -- Raman microscopy of bone / Simon R. Goodyear and Richard M. Aspden -- The calvarial injection assay / Robert J. van 't Hof -- Ovariectomy/orchidectomy in rodents / Aymen I. Idris -- Mechanical properties of bone ex vivo / Simon R. Goodyear and Richard M. Aspden -- Mechanical stimulation of bone cells using fluid flow / Carmen Huesa and Astrid D. Bakker -- Using cell and organ culture models to analyze responses of bone cells to mechanical stimulation / Andrew A. Pitsillides and Simon C.F. Rawlinson -- In vivo mechanical loading / Roberto Lopes de Souza and Leanne Saxon.
  • 2009 Springer
    edited by Antonio Giordano, Nicola Normanno.
  • 2007 Springer
    Syed Z. Ali, Anil V. Parwani ; with contributions by Maureen F. Zakowski and Edi Brogi.
  • 2015 ScienceDirect
    edited by Jennifer Ross, Wallace F. Marshall.
    Chapter 1. In vitro systems for the study of microtubule-based cell polarity in fission yeast -- Chapter 2. Microtubules, MAPs, and motor patterns -- Chapter 3. Self-organization of motors and microtubules in lipid-monolayered droplets -- Chapter 4. Reconstitution of microtubule-based motility using cell extracts -- Chapter 5. Building cells for quantitative, live-cell analyses of collective motor protein functions -- Chapter 6. Reconstituting cytoskeletal contraction events with biomimetic actin-myosin active gels -- Chapter 7. Building an artificial actin cortex on microscopic pillar arrays -- Chapter 8. Triggering actin polymerization in Xenopus egg extracts from phosphoinositide-containing lipid bilayers -- Chapter 9. Reconstituting geometry-modulated protein patterns in membrane compartments -- Chapter 10. Structural and functional studies of membrane remodeling machines -- Chapter 11. Building interconnected membrane networks -- Chapter 12. Using supported bilayers to study the spatiotemporal organization of membrane-bound proteins -- Chapter 13. Reconstituting ParA/ParB-mediated transport of DNA cargo -- Chapter 14. Cell-sized liposomes that mimic cell motility and the cell cortex -- Chapter 15. Reconstitution of cortical actin networks within water-in-oil emulsions -- Chapter 16. Engineering artificial cells by combining HeLa-based cell-free expression and ultrathin double emulsion template -- Chapter 17. Reconstitution of proteins on electroformed giant unilamellar vesicles -- Chapter 18. Reconstituting SNARE-mediated membrane fusion at the single liposome level.
  • 2012 ScienceDirect
    edited by Joel H. Rothman, Andrew Singson.
    An updated edition of the classic Methods in Cell Biology volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.
  • 2011 ScienceDirect
    edited by Joel H. Rothman, Andrew Singson.
    Pt.I Genetics and functional genomics : Mapping mutations in C. elegans / Eric J. Lambie -- Specialized chromosomes and their uses in caenorhabditis elegans / Martin R. Jones, Zoe Lohn, Ann M. Rose -- Genome engineering by transgene-instructed gene conversion in C. elegans / Valérie J.P. Robert, Jean-Louis Bessereau -- RNAi methods and screening : RNAi based high-throughput genetic interaction screening / Patricia G. Cipriani, Fabio Piano -- Dissection of genetic pathways in C. elegans / Zheng Wang, David R. Sherwood -- Pt.II Molecular biology and biochemistry : Transgenesis in C. elegans / Vida Praitis, Morris F. Maduro -- RNA processing in C. elegans / J. Jason Morton, Thomas Blumenthal -- Analysis of microRNA expression and function / Priscilla M. Van Wynsberghe, Shih-Peng Chan, Frank J. Slack, Amy E. Pasquinelli -- In situ hybridization of embryos with antisense RNA probes / Gina Broitman-Maduro, Morris F. Maduro -- Gene-centered regulatory network mapping / Albertha J.M. Walhout -- Affinity purification of protein complexes in C. elegans / Esther Zanin, Julien Dumont, RetoGassmann, Iain Cheeseman, Paul Maddox, Shirin Bahmanyar, Ana Carvalho, Sherry Niessen, John R. Yates III, Karen Oegema, Arshad Desai -- Pt.III Development : Cell indentification and cell lineage analysis / Claudiu A. Giurumescu, Andrew D. Chisholm -- The genetics and cell biology of fertilization / Brian D. Geldziler, Mathew R. Marcello, Diane C. Shakes, Andrew Singson -- Imaging embryonic morphogenesis in C. elegans / Jeff Hardin -- Molecular and genetic approaches for the analysis of C. elegans neuronal development / Dong Yan, Yishi Jin -- Methods for evaluating the caenorhabditis elegans dauer state : standard dauer-formation assay using synthetic daumones and proteomic analysis of O-GIcNAc modifications / Jeeyong Lee, Kwang-Youl Kim, Kyoe-Jin Joo, Keekyeong Kim, Pan-Young Jeong, Young-Ki Paik.
  • 2016 Springer
    Juan A. Rosado, editor.
    Historical overview of store-operated Ca2+ entry -- The STIM1- Orai interaction -- The TRPCs, Orais and STIMs in ER/PM junctions -- The TRP-Na-Ca2+ exchanger coupling -- Role of TRPC channels in store-operated calcium entry -- Phospholipase A2 as a molecular determinant of store-operated calcium entry -- Extracellular calcium has multiple targets to control cell proliferation -- Regulation of platelet function by Orai, STIM and TRP -- On the roles of the Transient Receptor Potential Canonical 3 (TRPC3) channel in endothelium and macrophages: implications in atherosclerosis -- Second messenger-operated calcium entry through TRPC6 -- Transient Receptor Potential Canonical 7 (TRPC7), a Calcium (Ca2+) Permeable Nonselective Cation Channel -- Calcium entry through thermosensory channels -- Calcium signalling through ligand-gated ion channels such as P2X1 receptors in the platelet and other non-excitable cells -- The calcium entry-calcium refilling coupling -- Microdomains associated to lipid rafts -- Role of scaffolding proteins in the regulation of TRPC-dependent calcium entry -- Modulation of calcium entry by mitochondria -- Modulation of calcium entry by the endo-lysosomal system -- Remodeling of calcium entry pathways in cancer.
    Also available: Print – 2016
  • 2012 Springer
    Lee Yee-Ki, Siu Chung-Wah.
    Calcium Handling in hiPSC-Derived Cardiomyocytes -- Myocardial Infarction and Heart Failure -- Embryonic Stem Cells/Pluripotent Stem Cells -- Human Induced Pluripotent Stem Cell (hiPSC)-Derived Cardiomyocytes as a Source for Cardiac Regeneration -- Spontaneous Cardiac Differentiation -- Mesoderm Enrichment by Wnt-Signaling Growth Factors -- Endoderm-Like Cell (END)-2 Co-culture Method -- Calcium Homeostasis in Cardiomyocytes -- Sarcoplasmic Reticulum (SR) Governs Maturity of Cardiomyocytes -- SR Junctional Proteins Play a Role in Calcium Flux Between Cytosol and SR -- Spatial and Temporal Ca2+ Wavefront Dictated by T-Tubule Structure -- IP3-Mediated Calcium Release Contributes to Whole-Cell Calcium Transients -- Calcium Handling Properties of hES-Derived Cardiomyocytes -- Calcium Handling Properties of hiPSC-Derived Cardiomyocytes -- iPSC-Derived Cardiomyocyte as a Potential Platform for Disease Modeling of the Impaired Calcium Handling-Related Syndrome -- Disease Modeling of Catecholaminergic Polymorphic Ventricular Tachycardia (CPVT) -- Mitochondrial Dysfunction-Related Cardiomyopathy-Impaired SR Ca2+ Reuptake -- Measurement of Cytosolic [Ca2+] by Fluorescence Confocal Microscopy -- Materials -- Calibration of Fluo-3 -- Isolation of hiPSC- and hESC-Derived Cardiomyocytes -- Recording of Cytosolic [Ca2+] -- RT-qPCR of Calcium Handling Proteins.
  • 2010 ScienceDirect
    edited by Michael Whitaker.
    Chapter 1. A Practical Guide to the Preparation of Ca2+ Buffers / Donald M. Bers, Chris W. Patton, Richard Nuccitelli -- Chapter 2. Photorelease Techniques for Raising or Lowering Intracellular Ca2+ / Robert Zucker -- Chapter 3. Making and Using Calcium-Selective Mini- and Microelectrodes / L. Hove-Madsen, S. Baudet, D.M. Bers -- Chapter 4. Construction, Theory, and Practical Considerations for using Self-referencing of Ca2+-Selective Microelectrodes for Monitoring Extracellular Ca2+ Gradients / Mark A. Messerli, Peter J.S. Smith -- Chapter 5. Practical Aspects of Measuring Intracellular Calcium Signals with Fluorescent Indicators / Joseph P.Y. Kao, Gong Li, Darryl A. Auston -- Chapter 6. Genetically Encoded Probes for Measurement of Intracellular Calcium / Michael Whitaker -- Chapter 7. Patch Clamp Methods for Studying Calcium Channels / David L. Armstrong, Christian Erxleben, Jody A. White -- Chapter 8. Nuclear Patch-Clamp Recording from Inositol 1,4,5-Trisphosphate Receptors / Taufiq Rahman, Colin W. Taylor -- Chapter 9. Confocal and Multiphoton Imaging of Intracellular Ca2+ / Godfrey Smith, Martyn Reynolds, Francis Burton, Ole Johan Kemi -- Chapter 10. The Use of Aequorins to Record and Visualize Ca2+ Dynamics: From Subcellular Microdomains to Whole Organisms / Sarah E. Webb, Kelly L. Rogers, Eric Karplus, Andrew L. Miller.
  • 2010 Springer Protocols
    edited by Alexei Verkhratsky and Ole H. Petersen.
    Principles of the CA2+ homeostatic/signalling system / A. Verkhratsky and O.H. Petersen -- CA2+ recordings: Hardware and software (from microscopes to cameras) / E.C. Toescu and J. Graham -- CA2+ imaging: Principles of analysis and enhancement / F. Mammano and M. Bortolozzi -- Bioluminescent CA2+ indicators / L. Fedrizzi and M. Brini -- Monitoring calcium levels with genetically encoded indicators / O. Garaschuk and O. Griesbeck -- Intracellular calcium-sensitive microelectrodes / R.C. Thomas -- CA2+ caging and uncaging / S.H. Kim and M.K. Park -- CA2+ imaging of intracellular organelles: Endoplasmic reticulum / R. Blum, O.H. Petersen and A. Verkhratsky -- CA2+ imaging of intracellular organelles: Mitochondria / L. Núñez [and others] -- CA2+ imaging of dendrites and spines / K. Holthoff -- In vivo CA2+ imaging of the living brain using multi-cell bolus loading technique / G. Eichhoff [and others] -- CA2+ imaging of glia / C. Lohr and J.W. Deitmer.
  • 2012 Springer
    Md. Shahidul Islam, [editor].
    "This volume ... [explores] topics ranging from the fundamental aspects of calcium signaling to its varied clinical implications. It presents comprehensive discussion of cutting-edge research alongside detailed analysis of critical issues, at the same time as setting out testable hypotheses that point the way to future scientific endeavors. The contributions feature material on theoretical and methodological topics as well as related subjects including mathematical modeling and simulations. They examine calcium signaling in a host of contexts, from mammalian cells to oocytes, zebrafish and plants. With much of interest to newcomers to the field as well as seasoned experts, this new publication is both wide-ranging and authoritative." --Excerpt from publisher's description.
    Also available: Print – 2012
  • 2013 Springer
    Lars Kaestner.
    Summary -- Introduction -- Direct evidence - the digital approach -- Fluorescence-based visualisation -- Aequorin-based measurements -- Measurement of calcium transport across membranes -- Molecular biology based approaches -- Manipulation of calcium -- Calcium-induced function -- Calcium signalling in cardiac myocytes -- Calcium signalling in red blood cells -- Perspective -- Calcium Signalling Methodology -- Non-linear and ultra high-speed imaging for explorations of the murine and human heart -- A system for optical high resolution screening of electrical excitable cells -- Concepts for optical high content screens of excitable primary isolated cells -- A primary culture system for sustained expression of a calcium sensor in preserved adult rat ventricular myocytes -- Calcium imaging of individual erythrocytes: Problems and approaches -- Calcium Signalling in Cardiac Myocytes -- Reduced Cardiac L-Type Ca²+ Current in Cav€ ₂ -/- Embryos Impairs Cardiac Development and Contraction With Secondary Defects in Vascular Maturation -- Overexpression of junctin causes adaptive changes in cardiac myocyte Ca²+ signaling -- Remodelling of Ca²+ handling organelles in adult rat ventricular myocytes during longterm culture -- Functional and morphological preservation of adult ventricular myocytes in culture by sub-micromolar cytochalasin D supplement -- Calcium Signalling in Red Blood Cells -- The non-selective voltage-activated cation channel in the human red blood cell membrane: reconciliation between two conflicting reports and further characterisation -- Ion channels in the human red blood cell membrane: Their further investigation and physiological relevance -- Prostaglandin E₂ activates channelmediated calcium entry in human erythrocytes: An indication for a blood clot formation supporting process -- Functional NMDA receptors in rat erythrocytes -- Stimulation of human red blood cells leads to Ca²+-mediated intercellular adhesion -- Lysophospatidic acid induced red blood cell aggregation in vitro -- Regulation of phosphatidylserine exposure in red blood cells -- Cation Channels in Erythrocytes - Historical and Future Perspective.
  • 2007 Springer
    edited by Ernesto Carafoli and Marisa Brini.
    Annexinopathies / M.J. Hayes ... [et al.] -- Calpains and human disease / I. Bertipaglia and E. Carafoli -- Gelsolin and diseases / L. Spinardi and W. Witke -- Guanylate cyclase-activating proteins and retina disease / W. Baehr and K. Palczewski -- Pathologies involving the S100 proteins and rage / C.W. Heizmann ... [et al.] -- The calcium-sensing receptor: Physiology, pathophysiology and CaR-based therapeutics / E.M. Brown -- Physiological roles of the Ca2+/CaM-dependent protein kinase cascade in health and disease / J. Colomer and A.R. Means -- Calcium channelopathies: Voltage-gated calcium channels / P.J. Adams and T.P. Snutch -- TRP channels in disease / S.E. Jordt and B.E. Ehrlich -- Diseases associated with altered ryanodine receptor activity / W.J. Durham ... [et al.] -- Inositol 1,4,5-tripshosphate receptor, calcium signalling and Huntington's disease / I. Bezprozvanny -- SERCA pumps and human diseases / A. Hovnanian -- The plasma membrane calcium ATPase and disease / B.L. Tempel and D.J. Shilling -- Diseases involving the Golgi calcium pump / J. Vanoevelen ... [et al.] -- Calcium signalling and cancer cell growth / T. Capiod ... [et al.] -- Calcium misregulation and the pathogenesis of muscular dystrophy / F.W. Hopf ... [et al.] -- Calcium and cell death / A. Verkhratsky -- Calcium and cell death: The mitochondrial connection / P. Bernardi and A. Rasola -- Role of calcium in the pathogenesis of Alzheimer's disease and transgenic models / K.N. Green ... [et al.] -- Calcium and cardiomyopathies / E.G. Kranias and D.M. Bers -- Calcium signalling and calcium transport in bone disease / H.C. Blair ... [et al.].
  • 2015 Springer Protocols
    edited by Manuela Zaccolo.
    Simultaneous assessment of cAMP signaling events in different cellular compartments using FRET-based reporters / Alex Burdyga and Konstantinos Lefkimmiatis -- Recording intracellular cAMP levels with EPAC-based FRET sensors by fluorescence lifetime imaging / Marcel Raspe, Jeffrey Klarenbeek, and Kees Jalink -- Novel approach combining real-time imaging and the patch-clamp technique to calibrate FRET-based reporters for cAMP in their cellular microenvironment / Andreas Koschinski and Manuela Zaccolo -- Structure-based, in silico approaches for the development of novel cAMP FRET reporters / Matías Machado and Sergio Pantano -- Automated image analysis of FRET signals for subcellular cAMP quantification / Silas J. Leavesley [and others] -- Channel-based reporters for cAMP detection / Thomas C. Rich [and others] -- Imaging sub-plasma membrane cAMP dynamics with fluorescent translocation reporters / Anders Tengholm and Olof Idevall-Hagren -- Adenoviral transduction of FRET-based biosensors for cAMP in primary adult mouse cardiomyocytes / Oliver Lomas [and others] -- Generation of transgenic mice expressing FRET biosensors / Daniela Hübscher and Viacheslav O. Nikolaev -- Photoactivatable adenylyl cyclases (PACs) as a tool to study cAMP signaling in vivo : an overview / Marina Efetova and Martin Schwärzel -- Selective disruption of the AKAP signaling complexes / Eileen J. Kennedy and John D. Scott -- Screening for small molecule disruptors of AKAP-PKA interactions / Carolin Schächterle [and others] -- Structure-based bacteriophage screening for AKAP- selective PKA regulatory subunit variants / Ryan Walker-Gray and Matthew G. Gold -- Yeast-based high-throughput screen for modulators of phosphodiesterase activity / Ana Santos de Medeiros and Charles S. Hoffman -- Separation of PKA and PKG signaling nodes by chemical proteomics / Eleonora Corradini, Albert J.R. Heck, and Arjen Scholten -- Development of computational models of cAMP signaling / Susana R. Neves-Zaph and Roy S. Song.
  • 2011 Springer Protocols
    edited by Ian A. Cree.
    Xenotransplantation of breast cancers -- Feeder layers: Co-culture with nonneoplastic cells -- Drug design and testing: Profiling of antiproliferative agents for cancer therapy using a cell-based methyl-[3h]-thymidine incorporation assay -- Transfection and DNA-mediated gene transfer -- Analysis of drug interactions -- Development of rituximab-resistant B-NHL clones: An in vitro model for studying tumor resistance to monoclonal antibody-mediated immunotherapy -- Proteomic evaluation of cancer cells: Identification of cell surface proteins -- Measuring gene expression from cell cultures by quantitative reverse-transcriptase polymerase chain reaction -- Expression analysis of homeobox genes in leukemia/lymphoma cell lines -- Flow cytometric DNA analysis of human cancers and cell lines -- Angiogenesis assays -- Cell migration and invasion assays -- Molecular breakpoint analysis of chromosome translocations in cancer cell lines by long distance inverse-PCR -- Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay -- Purification of annexin V and its use in the detection of apoptotic cells -- Real-time cytotoxicity assays -- Differential staining cytotoxicity assay: A review -- Cell sensitivity assays: The ATP-based tumor chemosensitivity assay -- Cell sensitivity assays: The MTT assay -- Cellular chemosensitivity assays: An overview -- Isolation of endothelial cells from human tumors -- Isolation of inflammatory cells from human tumours -- Establishment and culture of leukemia-lymphoma cell lines -- Isolation and culture of ovarian cancer cells and cell lines -- Isolation and culture of squamous cell carcinoma lines -- Isolation and culture of melanoma and naevus cells and cell lines -- Isolation and culture of colon cancer cells and cell lines -- Generation of lung cancer cell line variants by drug selection or cloning -- Quality assurance and good laboratory practice -- Elimination of mycoplasmas from infected cell lines using antibiotics -- Detecting mycoplasma contamination in cell cultures by polymerase chain reaction -- Cell culture contamination -- Cytogenetic analysis of cancer cell lines -- Online verification of human cell line identity by STRDNA typing -- Characterization and authentication of cancer cell lines: An overview -- Storage of cell lines -- Principles of cancer cell culture -- Cancer biology.
  • 2011 Springer
    Glenn Dranoff, Editor.
    Immune infiltration in human cancer : prognostic significance and disease control / Wolf H. Fridman ... [et al.] -- Subversion and coercion : the art of redirecting tumor immune surveillance / John B. Mumm and Martin Oft -- STAT3 : a target to enhance antitumor immune response / Heehyoung Lee ... [et al.] -- Biology and clinical observations of regulatory T cells in cancer immunology / Michele W. Teng ... [et al.] -- Concepts and ways to amplify the antitumor immune response / Bent Rubin and Jean Edouard Gairin -- Angiogenesis and the tumor vasculature as antitumor immune modulators : the role of vascular endothelial growth factor and endothelin / Lana E. Kandalaft ... [et al.] -- Adoptive cellular therapy / Stephen A. Grupp and Carl H. June -- Dendritic cell subsets as vectors and targets for improved cancer therapy / Karolina Palucka ... [et al.] -- Identification of human idiotype-specific T cells in lymphoma and myeloma / Jinsheng Weng ... [et al.] -- Modulation of CTLA-4 and GITR for Cancer Immunotherapy / Francesca Avogadri ... [et al.] -- Immunobiology of cancer therapies targeting CD137 and B7-H1/PD-1 cosignal pathways / Shengdian Wang and Lieping Chen -- LAG-3 in cancer immunotherapy / Monica V. Goldberg and Charles G. Drake -- Immunologically active biomaterials for cancer therapy / Omar A. Ali and David J. Mooney -- Erratum : Identification of human idiotype-specific T cells in lymphoma and myeloma / Jinsheng Weng ... [et al.].
    Also available: Print – 2011
  • 2009 Springer
    Gianfranco Baronzio, Giammaria Fiorentini, Christopher R. Cogle, editors.
  • 2014 Wiley
    editor, Vinagolu K. Rajasekhar.
    Oxytocin and vasopressin systems: oxytocin, vasopressin, and their interplay with gonadal steroids: essentials of cancer stem cells and conceptual modeling -- Theoretical and experimental foundations of the "cancer stem cell" model -- The hallmarks of prostate cancer stem cells -- Self-renewal, induced proliferation, and autonomous cell growth represent distinct modes of cell multiplication: relevance to the cancer stem cell theory -- Human embryonic stem cells and cancer: modeling disease in a dish -- Cancer stem cell as a result of a reprogramming-like mechanism: implications in tumor development and treatment -- A cancer stem cell model: an insight into the conversion of induced pluripotent stem cells to cancer stem-like cells -- Altruistic stem cells and cancer stem cells -- The emerging concept of EMT-induced cancer stem cells -- Models to study chronic myeloid leukemia cancer stem cells -- Cancer stem cells in melanoma: biomarkers and mathematical models -- Stem cells in liquid tumors -- Acute myeloid leukemia stem cells: updates and controversies -- Leukemia-initiating cells in acute lymphoblastic leukemia -- Stem cells in solid tumors -- Lung cancer stem cells and resistance to radiotherapy -- Prostate cancer cell heterogeneity and prostate cancer stem cells -- Glioblastoma stem cells drive tumor recurrence and patient relapse: what's the evidence? -- Stem cells and pancreatic cancer -- Melanoma subpopulations with cancer stem cell phenotypes -- Sarcoma stem cells -- Cancer stem cells in tumor metastasis perspective -- Cancer stem cells in metastasis and minimal residual disease -- Role of cancer stem cells in metastasis -- Cancer stem cells and the stromal microenvironment -- A perspective on breast cancer malignant progression: from cancer stem cell intra tumor heterogeneity to metastasis-initiating cells -- Novel and potential targets in cancer stem cells -- Targeting cancer stem cells: modulating embryonic stem cell signaling, epigenetics, and tumor metabolism -- Oct4, Oct1, and cancer stem cells -- The role of Cripto-1 in cancer and cancer stem cells -- Leptin signaling in the regulation of stem and cancer stem cells -- Tumor-initiating stem-like cells: carcinogenesis through toll-like receptors, environmental factors, and virus -- The role of epithelial cell polarity pathways on cancer stem cells -- Cancer-initiating cells, exosomes, and the premetastatic niche -- Microrna therapeutics to target brain tumor stem cells -- The riboproteome orchestrates self-renewal and cell fate in leukemia -- Clinical relevance of cancer stem cells in patients -- Targeting different states of breast cancer stem cells -- Difficulties in targeting the beating heart: therapeutic implications of the cancer stem cell hypothesis in melanoma -- Targeting cancer stem cells for overcoming drug resistance and cancer progression -- The role of cancer stem cells in tumor radioresistance.
  • 2011 Springer
    Alison L. Allan, editor.
    Cancer stem cells : historical perspectives and lessons from leukemia / Christopher R. Cogle -- Cancer stem cells in breast cancer / Jenny E. Chu and Alison L. Allan -- Cancer stem cells in brain cancer / Xin Wang, Chitra Venugopal, and Sheila K. Singh -- Cancer stem cells in colorectal cancer / Mauro Biffoni, Eros Fabrizi, and Lucia Ricci-Vitiani -- Cancer stem cells in pancreatic cancer / Jorge Dorado, Alicia G. Serrano, and Christopher Heeschen -- Cancer stem cells in prostate cancer / Paula Kroon ... [et al.] -- Cancer stem cells in melanoma / Ping Jin ... [et al.] -- Cancer stem cells in lung cancer / Jun Shen and Feng Jiang -- Cancer stem cells in ovarian cancer / Fang Fang ... [et al.] -- Cancer stem cells in hepatocellular cancer / Russell C. Langan and Itzhak Avital -- Cancer stem cells in head and neck cancer / Mark E.P. Prince and Samantha J. Davis -- Relationship between regulatory pathways in pluripotent stem cells and human tumors / Olga Gaidarenko and Yang Xu -- Influence of the embryonic microenvironment on tumor progression / Daniela Quail ... [et al.] -- The epithelial-to-mesenchymal transition and cancer stem cells / Jonas Fuxe -- Application of stem cell assays for the characterization of cancer stem cells / Pamela M. Willan and Gillian Farnie -- Zebrafish as a model to study stem cells in development, disease, and cancer / Viviana Anelli, Cristina Santoriello, and Marina C. Mione -- Imaging cancer stem cells / Paula Foster -- Mouse models for studying normal and cancer stem cells / David A. Hess -- Cancer stem cells and disease prognosis / Zeshaan A. Rasheed, Jeanne Kowalski, and William H. Matsui -- Mechanisms of radioresistance in cancer stem cells / Cleo Y-F Lee and Maximilian Diehn -- The role of ABC transporters in cancer stem cell drug resistance / Vera S. Donnenberg, Ludovic Zimmerlin, and Albert D. Donnenberg -- Resistance to endocrine therapy in breast cancer : are breast cancer stem cells implicated? / Ciara S. O'Brien ... [et al.] -- Future directions : cancer stem cells as therapeutic targets / Alysha K. Croker and Alison L. Allan -- Final thoughts : complexity and controversy surrounding the "cancer stem cell" paradigm / Craig Gedye, Richard P. Hill, and Laurie Ailles.
  • 2007 Springer
    O.D. Wiestler, B. Haendler, D. Mumberg, eds.
  • 2015 Springer Protocols
    edited by Gary R. Skuse and Maureen C. Ferran.
    This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics, and function of cardiomyocytes. The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes, and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and current, Cardiomyocytes: Methods and Protocols explores complimentary areas of cardiomyocyte science that, taken together, can inform individuals with a broad range of interests.
  • 2014 Springer Protocols
    edited by Peter V. Bozhkov, Department of Plant Biology, Uppsa;a BioCenter, Swedish University of Agricultural Science and Linnean Center for Plant Biology, Uppsala, Sweden, Guy Salvesen, Sanford-Burnham Medical Research Institute, La Jolla, CA, USA.
    General in vitro Caspase Assay Procedures -- Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity.- Global Identification of Caspase Substrates using PROTOMAP (PROtein TOpography and Migration Analysis Platform) -- Caspase-2 Protocols -- Caspase-14 Protocols -- Caspase Protocols in Caenorhabditis elegans -- Detecting Caspase Activity in Drosophila Larval Imaginal Discs -- Methods for the Study of Caspase Activation in the Xenopus laevis Oocyte and Egg Extract -- Caspase Protocols in Mice -- Measurement of Caspase Activation in Mammalian Cell Cultures -- Detection and Measurement of Paracaspase MALT1 Activity -- Leishmania Metacaspase: an Arginine-Specific Peptidase -- Purification, Characterization, and Crystallisation of Trypanosoma Metacaspases -- Monitoring the Proteostasis Function of the Saccharomyces cerevisiae Metacaspase Yca1 -- Plant Metacaspase Activation and Activity -- Preparation of Arabidopsis thaliana Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC.
  • 2012 Springer
    edited by Jean-François Jasmin, Philippe G. Frank, Michael P. Lisanti.
    Lipid rafts, caveolae, and GPI linked proteins / Valerie L. Reeves, Candice M. Thomas, and Eric J. Smart -- Caveolae and the regulation of endocytosis / Anna L. Kiss -- Caveolin : role in cell signaling / Cecile Boscher and Ivan Robert Nabi -- Regulation of ENOS in caveolae / Chieko Mineo and Philip W. Shaul -- Recent developments in the interactions between caveolin and pathogens / Fabiana S. Machado ... [et al.] -- Caveolin and breast cancer : a new clinical perspective / Isabelle Mercier and Michael P. Lisanti -- Caveolin and prostate cancer progression / Michael R. Freeman, Wei Yang, and Dolores Di Vizio -- Caveolins and caveolae, roles in insulin signalling, and diabetes / Peter Strolfors -- Atherosclerosis, caveolae, and caveolin / Stephanos Pavlides ... [et al.] -- Caveolins and heart diseases / Mathivadhani Panneerselvam, Hemal H. Patel, and David M. Roth -- Caveolins and lung function / Nikolaos A. Maniatis ... [et al.].
    Also available: Print – 2012
  • 2011
    Michael Nathaniel Alonso.
    Monocytes rapidly infiltrate inflamed tissues and differentiate into inflammatory dendritic cells (DCs) that mediate pathogen clearance. However, the cellular and molecular mechanisms governing inflammatory DC formation and subsequent function remain elusive. Current evidence suggests that conserved molecular patterns present on different classes of microbes that engage pattern recognition receptors on DCs dictate the type of immune response initiated by DCs. Given that pathogens are under evolutionary pressure to avoid elimination, we reasoned that memory T cells were better positioned to inform the immune response. The studies presented in this dissertation indicate that memory T cells dictate the nature of the immune response through the induction of functionally specialized inflammatory DCs. Our studies indicate that T-helper (TH) cells interact with monocytes in inflamed tissues and instruct monocytes to differentiate into inflammatory DCs in vivo. This process is antigen-driven and requires cell contact, GM-CSF and TNF[alpha]. Interestingly, distinct TH cell subsets direct the formation of specialized DC subsets. In psoriasis lesions, TH1 and TH17 cells interact with monocytes and instruct these cells to differentiate into TH1- and TH17-promoting DCs, respectively. Correspondingly, in acute atopic dermatitis, TH2 cells interact with monocytes and elicit the formation of TH2-promoting DCs. Again, DC formation required GM-CSF and cell contact, whereas TH subset specific cytokines dictate DC function and the expression of DC subset specific surface molecules. Moreover, the phenotypes of T cell induced DC subsets are maintained following subsequent stimulation with a panel of toll-like receptor (TLR) agonists, suggesting that TH-derived signals outweigh downstream TLR signals in their influence on DC function. Our preliminary studies indicate that regulatory T cells promote the formation of tolerogenic DCs that are typically associated with tumor progression and poor patient prognosis. The data indicate that intra-tumoral Tregs frequently interact with monocytes within tumor tissue and instruct monocytes to differentiate into IL-10-producing tolerogenic DCs. Such Treg-induced DCs (DCTreg) are subsequently able to instruct naïve T cells to differentiate into FoxP3+, IL-10-producing regulatory T cells with potent suppressive activity. The capacity of Tregs to elicit DCTreg formation represents a physiologic mechanism that on one hand amplifies and perpetuates immune tolerance, but also contributes to attenuated anti-tumor immune responses. We also explored mechanisms capable of depleting inflammatory DCs. While previous studies have indicated that inflammatory DC depletion through systemic elimination of their monocyte precursors with clodronate-loaded liposomes ameliorates pathogenesis in psoriasis and other diseases, translation of these strategies into the clinic is difficult due the importance of monocytes in the clearance of infection. We describe three strategies that avoid the monocyte intermediates in order to directly prevent inflammatory DC formation or deplete inflammatory DCs through antibody-loaded toxins. These strategies provide useful tools for the targeted reduction of inflammatory DCs during disease pathogenesis. In summary, memory T cells dictate the nature of the immune response through the induction of functionally specialized inflammatory DCs. The system we utilized for generating DC subsets with differential TH polarizing capacity has facilitated the elucidation of cell surface and soluble factors governing the biology of TH-promoting DC subsets. These and additional molecules expressed by DC subsets should prove useful for detecting and quantifying each subset in situ and may enable the design of novel therapeutic strategies for T[not]H-associated inflammatory diseases.
  • 2006 Springer
    edited by Anne E. Cress and Raymond B. Nagle.
  • 2014 Springer
    Christian Behl, Christine Ziegler.
    Aging and cell aging : an introduction -- Cell cycle : the life cycle of a cell -- Theories and mechanisms of aging -- Selected age-related disorders.
  • 2013 Springer
    Heide Schatten, editor.
    Histopathology and grading of breast cancer / Magda Esebua -- Multicentric/multifocal breast cancer : overview, biology, and therapy / Federico Buggi ... [et al.] -- Immune system in breast cancer initiation and progression : role of epithelial to mesenchymal transition / Keith L. Knutson and Erik C. Radisky -- Remodelling of the extracellular matrix : implication for cancer / Thomas R. Cox and Janine T. Erler -- Biology and treatment of basal-like breast cancer / Bingchen Han ... [et al.] -- Re-excision after lumpectomy for breast cancer / Suzanne B. Coopey -- Novel anti-angiogenic therapies using naturally occurring and synthetic drugs to combat progestin-dependent breast cancer / Salman M. Hyder, Benford Mafuvadze, and Cynthia Besch-Williford -- New insights on estrogen receptor actions in hormone-responsive breast cancer cells by interaction proteomics / Concetta Ambrosino ... [et al.] -- Reprogramming breast cancer cells with embryonic microenvironments : insights from nodal signaling / Gina Kirsammer and Mary J.C. Hendrix - Metastatic determinants : breast tumour cells in circulation / Nisha Kanwar and Susan J. Done -- Breast cancer epigenetics : biomarkers and therapeutic potential / Nancy H. Nabilsi ... [et al.] -- Impact of centrosome abnormalities on breast cancer development and progression with a focus on targeting centrosomes for breast cancer therapy / Heide Schatten -- New perspective on cyclin D1 : beyond cell cycle regulation / Chenguang Wang, Timothy G. Pestell, and Richard G. Pestell -- Gene signatures of inflammatory breast cancer : epithelial plasticity and a cancer stem cell phenotype / Fredika M. Robertson ... [et al.] -- Integrated human mammary epithelial cell culture system for studying carcinogenesis and aging / Martha R. Stampfer, Mark A. LaBarge, and James C. Garbe -- New breast cancer treatment considerations a brief review of the use of genetically modified (attenuated) bacteria as therapy for advanced and metastatic breast cancer / Robert A. Kazmierczak ... [et al.].
  • 2007 Springer
    Ralph Bock (ed.).
  • 2002 ScienceDirect
    edited by Brian Matsumoto.
    Also available: Print – 2002
  • 2006 ScienceDirect
    edited by Julio E. Celis.
  • 2010 Springer
    edited by Eran Meshorer, Kathrin Plath.
    Early embryonic cell fate decisions in the mouse / Yojiro Yamanaka and Amy Ralston -- Nuclear architecture in stem cells / Kelly J. Morris, Mita Chotalia, and Ana Pombo -- Epigenetic regulation of pluripotency / Eleni M. Tomazou and Alexander Meissner -- Autosomal lyonization of replication domains during early mammalian development / Ichiro Hiratani and David M. Gilbert -- Preservation of genomic integrity in mouse embryonic stem cells / Peter J. Stambrook and Elisia D. Tichy -- Transcriptional regulation in embryonic stem cells / Jian Chien, Dominic Heng, and Huck Hui Ng -- Alternative splicing in stem cell self renewal and differentiation / David A. Nelles and Gene W. Yeo -- MicroRNA regulation of embryonic stem cell self renewal and differentiation / Collin Melton and Robert Blelloch -- Telomeres and telomerase in adult stem cells and pluripotent embryonic stem cells / Rosa M. Marión and Maria A. Blasco -- X chromosome inactivation and embryonic stem cells / Tahsin Stefan Barakat and Joost Gribnau -- Adult stem cells and their niches / Francesca Ferraro, Cristina Lo Celso, and David Scadden -- Adult stem cell differentiation and trafficking and their implications in disease / Ying Zhuge, Zhao-Jun Liu, and Omaida C. Velazquez -- Vertebrates that regenerate as models for guiding stem cells / Christopher L. Antos and Elly M. Tanaka -- Reprogramming of somatic cells to pluripotency / Masato Nakagawa and Shinya Yamanaka.
    Also available: Print – 2010
  • 2009 Springer
    Edward Koenig (ed.).
    Also available: Print – 2009
  • 2006 Springer
    Eckart D. Gundelfinger, Constanze I. Seidenbecher, Buckhart Schraven (eds.).
    Also available: Print – 2006
  • 2011 Springer Protocols
    edited by Michael Aschner, Cristina Suñol, Anna Bal-Price.
    Guidance on Good Cell Culture Practice (GCCP) -- Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology -- Neural Stem Cells -- Primary Cultures for Neurotoxicity Testing -- Preparation and Use of Serum-Free Aggregating Brain Cell Cultures for Routine Neurotoxicity Screening -- Cell Culture to Investigate Neurotoxicity and Neurodegeneration Utilizing Caenorhabditis elegans -- Modeling the Blood-Brain Barrier -- In vitro Models of the Blood-Cerebrospinal Fluid Barrier and Their Use in Neurotoxicological Research -- Introducing Cloned Genes into Cultured Neurons Providing Novel In vitro Models for Neuropathology and Neurotoxicity Studies -- P19 Embryonic Carcinoma Cell Line: A Model To Study Gene-Environment Interactions -- Signal Transduction and Neurotoxicity: What Can We Learn from Experimental Culture Systems? -- Neurite Degeneration in Human Neuronal SH-SY5Y Cells as an Indicator of Axonopathy -- The Use of Differentiating N2a and C6 Cell Lines for Studies of Organophosphate Toxicity -- Assessing Toxic Injuries of Experimental Therapeutics to the Crystalline Lens Using Lens Explant Culture -- Necrosis, Apoptosis, and Autophagy: Mechanisms of Neuronal and Glial Cell Death -- Inflammation and Reactive Oxygen/Nitrogen Species in Glial/Neuronal Cultures -- Neuronal Oxidative Injury and Biomarkers of Lipid Peroxidation -- Analysis of Protein Targets by Oxidative Stress Using the Oxyblot and Biotin-Avidin-Capture Methodology -- Catecholaminergic Cell Lines for the Study of Dopamine Metabolism and Neurotoxicity -- 13C NMR Spectroscopy and Mass Spectrometry Analysis of Intermediary Metabolism in Cultured Neural Cells -- Culture Models for the Study of Amino Acid Transport and Metabolism -- Neurotransmitter Transporters and Anticonvulsant Drug Development -- Ion Channel Electrophysiology in Cultured Neurons -- Neurotoxicity Assessment by Recording Electrical Activity from Neuronal Networks on Microelectrode Array Neurochips -- GABAA Receptor Binding and Ion Channel Function in Primary Neuronal Cultures for Neuropharmacology/Neurotoxicity Testing.
  • 2005 CRCnetBASE
    edited by Sadettin S. Ozturk, Wei-Shou Hu.
  • 2014 Springer Protocols
    edited by Eishi Noguchi and Mariana C. Gadaleta.
    Cell cycle-regulated transcription : effectively using a genomics toolbox / Sara L. Bristow, Adam R. Leman, and Steven B. Haase -- Cell cycle regulation by checkpoints / Kevin J. Barnum and Matthew J. O'Connell -- Interplacy between the cell cycle and double-strand break response in mammalian cells / Kate Beishline and Jane Azizkhan-Clifford -- Cell cycle regulation by protein degradation / Deanna M. Koepp -- Linking chromosome duplication and segregation via sister chromatid cohesion / Adam R. Leman and Eishi Noguchi -- The greatwall : PP2A axis in cell cycle control / Peng Wang, Marcos Malumbres, and Vincent Archambault -- Cell cycle regulation by the nutrient-sensing mammalian target of rapamycin (mTOR) pathway / Elisabet Cuyàs ... [and 3 others] -- The senescence arrest program and the cell cycle / Alessandro Bitto ... [and 4 others] -- Oncogenic Ras pushes (and pulls) cell cycle progression through ERK activation / Paul M. Campbell -- Cell cycle regulation during viral infection / Sumedha Bagga and Michael J. Bouchard -- The roles of cohesins in mitosis, meiosis, and human health and disease / Amanda S. Brooker and Karen M. Berkowitz -- Introductory review of computational cell cycle modeling / Andres Kriete, Eishi Noguchi, and Christian Sell -- Cell cycle synchronization and flow cytometry analysis of mammalian cells / Naoko Yoshizawa-Sugata and Hisao Masai -- Analyzing transcription dynamics during the budding yeast cell cycle / Adam R. Leman, Sara L. Bristow, and Steven B. Haase -- Analyzing cell cycle checkpoints in response to ionizing radiation in mammalian cells / Bin Wang -- Analyzing DNA replication checkpoint in budding yeast / Nicole Hustedt and Kenji Shimada -- Analyzing cell cycle-dependent degradation and ubiquitination in budding yeast / Dong-Hwan Kim and Deanna M. Koepp -- Imaging analysis of cell cycle-dependent degradation of Cdt1 in mammalian cells Yasushi Shiomi ... [and 4 others] -- PCNA-dependent ubiquitination of Cdt1 and p21 in mammalian cells / Akiyo Hayashi ... [and 3 others] -- Analyzing Cdc2/Cdk1 activation during stress response in Schizosaccharomyces pombe / Miguel A. Rodríguez-Gabriel -- Analyzing Ras-associated cell proliferation signaling / Matthew C. Stout ... [and 4 others] -- Telomere regulation during the cell cycle in fission yeast / Bettina A. Moser, Ya-Ting Chang, and Toru M. Nakamura -- Detecting senescence : methods and approaches / Elizabeth P. Crowe ... [and 5 others] -- Analyzing RB and E2F during the G1-S transition / Michael J. Thwaites, Matthew J. Cecchini, and Frederick A. Dick -- Analyzing G1-S transcriptional control / Steffi Klier, Sarah Farmer, and Robertus A.M. de Bruin -- Analysis of replication timing using synchronized budding yeast cultures / Jie Peng, M.K. Raghuraman, and Wenyi Feng -- Analysis of ssDNA gaps and DSBs in genetically unstable yeast cultures / Jie Peng, M.K. Raghuraman, and Wenyi Feng -- Chromatin fractionation analysis of licensing factors in mammalian cells / Hideo Nishitani ... [and 4 others] -- Imaging analysis to determine chromatin binding of the licensing factor MCM2-7 in mammalian cells / Masayuki Morino ... [and 3 others] -- Chromatin immunopreciptiation to investigate origin association of replication factors in mammalian cells / Adam R. Leman and Eishi Noguchi -- Live-cell fluorescence imaging for phenotypic analysis of mitosis / Sushama Sivakumar, John R. Daum, and Gary J. Gorbsky -- Analyzing sister chromatid cohesion in mammalian cells / Katherine M. Feeney, Laura McFarlane-Majeed, and Joanna L. Parish -- Affinity purification of protein complexes from Drosophila embryos in cell cycle studies / Zoltan Lipinszki ... [and 8 others] -- Tracking histone variant nucleosomes across the human cell cycle using biophysical, biochemical, and cytological analyses / Marcin P. Walkiewicz ... [and 3 others].
  • 2010 Springer
    Greg H. Enders, editor.
    Part I: Starting the Cell Division Cycle -- 1 Escape from Cellular Quiescence -- 2 Interplay Between Cyclin-Dependent Kinases and E2F-Dependent Transcription -- 3 Regulation of Pre-RC Assembly: A Complex Symphony Orchestrated by CDKs -- Part II: Proliferation Under Duress -- 4 Mitotic Checkpoint and Chromosome Instability in Cancer -- 5 Mitotic Catastrophe -- 6 p53, ARF, and the Control of Autophagy -- Part III: Long-Term Proliferation -- 7 Regulation of Self-Renewing Divisions in Normal and Leukaemia Stem Cells -- 8 Maintenance of Telomeres in Cancer -- 9 The Senescence Secretome and Its Impact on Tumor Suppression and Cancer -- Part IV: Applications in Preventing and Treating Cancer -- 10 Cell Cycle Deregulation in Pre-neoplasia: Case Study of Barrett8217;s Oesophagus -- 11 Targeting Cyclin-Dependent Kinases for Cancer Therapy -- Index.
  • 2010
    Stacey Ellen Wirt.
    The ability of stem/progenitor cells to exit the cell cycle is essential for proper embryonic development, but the mechanisms governing cell cycle exit are still not fully understood. Correct regulation of G1 is critical for ensuring proper organismal development, tissue homeostasis, and tumor suppression. The Retinoblastoma (Rb) gene family is composed of three genes, Rb, p107, and p130, which are collectively thought to serve as essential regulators of the G1 phase of the cell cycle in most proliferating cell types. The Rb family has also been implicated in promoting the gene expression of several differentiation programs in different cell lineages. However, due to the overlapping and compensatory functions among the Rb family members, a complete understanding of how the Rb family controls the cell cycle and differentiation programs within a cell remains unclear. Furthermore, it has not yet been tested whether the Rb family members are the only mediators of these cellular processes, or if there are alternative mechanisms for cell cycle exit and differentiation in mammalian stem and progenitor cells. Here we tested the requirement for the Rb protein and its family members p107 and p130 in G1 arrest and differentiation in mammalian cells. We employed several strategies to conditionally remove the Rb family genes in entire embryos and in specific neural lineages, as well as multiple differentiation systems in vitro. We found that Rb family triple knock-out (TKO) mouse embryos survive until days 9-11 of gestation, with similar patterning and organogenesis as seen in wild type embryos. Strikingly, a number of TKO cells, including in the neural lineage, are able to exit the cell cycle in G1 and fully differentiate in vivo and in culture. This ability of TKO cells to arrest in G1 is associated with the repression of key cell cycle target genes for E2F and Myc transcription factors. Thus, G1 arrest can be achieved independently of Rb family members, illustrating the robustness of cell cycle regulatory networks during embryogenesis, and identifying a novel program that regulates cell cycle exit in G1 in mammalian cells.
  • 2011 Springer
    Jacek Z. Kubiak, editor.
    Also available: Print – 2011
  • 2016 Springer Protocols
    edited by Amanda S. Coutts, Louise Weston.
  • 2006 Springer
    Philipp Kaldis (ed.).
    Also available: Print – 2006
  • 2010 Springer
    Antonio Giordano, Umberto Galderisi, editors.
  • 2011 Springer Protocols
    edited by Gaspar Banfalvi.
    1. Overview of cell synchronization / Gaspar Banfalvi -- 2. Synchronization of mammalian cells and nuclei by centrifugal elutriatio / Gaspar Banfalvi -- 3. Cytofluorometric purification of diploid and tetraploid cancer cells / Maria Castedo, Lorenzo Galluzzi, Ilio Vitale, Laura Senovilla, Didier Métivier, Mohamed Jèmaà, Santiago Rello-Varona, and Guido Kroemer -- 4. Large-scale mitotic cell synchronization / Kalyan Dulla and Anna Santamaria -- 5. Synchronization of mammalian cell cultures by serum deprivation / Thomas J. Langan and Richard C. Chou -- 6. Cell synchronization by inhibitors of DNA replication induces replication stress and DNA damage response: analysis by flow cytometry / Zbigniew Darzynkiewicz, H. Dorota Halicka, Hong Zhao, and Monika Podhorecka -- 7. Chromosome formation during fertilization in eggs of the teleost oryzias latipes / Takashi Iwamatsu -- 8. Specific cell cycle synchronization with butyrate and cell cycle analysis / Congjun Li -- 9. Analysis of nuclear uracil-DNA glycosylase (nUDG) turnover during the cell cycle / Jennifer A. Fischer and Salvatore Caradonna -- 10. Synchronization of HeLa cells / Hoi Tang Ma and Randy Y. C. Poon -- 11. Synchronization of Bacillus subtilis cells by spore germination and outgrowth / Gasper Banfalvi -- 12. Synchronization of yeast / Arkadi Manukyan, Lesley Abraham, Huzefa Dungrawala, and Brandt L. Schneider -- 13. Synchronization of pathogenic protozoans / Staffan Svärd and Karin Troell -- 14. Synchronization of in vitro maturation porcine oocytes / Tamas Somfai and Yuji Hirao -- 15. Synchronization of medicago sativa cell suspension culture / Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, and Gábor V. Horváth -- 16. Cell cycle synchronization for the purpose of somatic cell nuclear transfer (SCNT) / Yoel Shufaro and Benjamin E. Reubinoff -- 17. Ex vivo expansion of haematopoietic stem cells to improve engraftment in stem cell transplantation / Kap-Hyoun Ko, Robert Nordon, Tracey A. O'Brien, Geoff Symonds, and Alla Dolnikov -- 18. Flow cytometry developments and perspectives in clinical studies: examples in ICU patients / Fabienne Venet, Caroline Guignant, and Guillaume Monneret -- 19. Molecular network dynamics of cell cycle control: transitions to start and finish / Attila Csikász-Nagy, Alida Palmisano, and Judit Zámbprszky.
  • 2011 Springer
    by Gerardo H. Vázquez-Nin, María Luisa Escobar, M. De Felici, Olga Margarita Echeverría, Francesca Gioia Klinger.
    [Publisher-supplied data] The ovary is a suitable organ for studying the processes of cell death. Cell death was first described in the rabbit ovary (Graaffian follicles), the phenomenon being called chromatolysis. To date, it is recognized that various forms of cell death (programmed cell death, apoptosis and autophagy) are essential components of ovarian development and function. Programmed cell death is responsable for the ovarian endowment of primordial follicles around birth; in the prepuberal and adult period, apoptosis is a basic mechanism by which oocytes are eliminated by cancer therapies and environmental toxicants; in the ovarian cycle, follicular atresia and luteal regression involve follicular cell apoptosis. Finally, abnormalities in cell death processes may lead to ovarian disease such as cancer and chemoresistance. In this book, after an introductory description of various forms of cell death and of the ovary development and function in mammals, the processes of cell death in ovarian somatic cells and oocytes are described at cytological, physiological and molecular levels and analyzed in the embryonic, prepuberal and adult ovary. A complex array of molecular pathways triggered by extrinsic and intrinsic signals able tor induce or suppress cell death in the same cell, according to cell type and ovary developmental stage, emerges. Physiological interactions with the axis hypothalamus-hypophysis as well as ovarian internal functional signal are also critically reviewed to explain the abortive development of follicles before the beginning of the ovarian cycle. The book conveys information useful to the updating of biologists and physicians who are interested to the ovary biology and functions. Hopefully it should provide also clues for stimulating novel experiments in the study of cell death in the mammalian ovary still at an early stage.
  • 2014 Springer
    Hao Wu, editor.
    Beginning from centuries of anecdotal descriptions of cell death, such as those on the development of the midwife toad in 1842 by Carl Vogt, to modern-day investigations of cell death as a biological discipline, it has become accepted that cell death in multicellular organisms is a normal part of life. This book provides a comprehensive view of cell death, from its mechanisms of initiation and execution, to its implication in human disease and therapy. Physiological cell death plays critical roles in almost all aspects of biology, and the book details its roles in lymphocyte homeostasis, neuronal function, metabolism, and the DNA damage response.?When physiological cell death goes awry, diseases can arise, and cancer is presented as a central paradigm for the consequences of derangements in the interplay between cell survival and cell death. At the same time, the potential promise of targeted therapies aimed at interdicting cell death machineries are also discussed extensively. The molecular mechanisms that underlie apoptotic cell death are illustrated from the perspectives of both the intrinsic, mitochondrial apoptotic pathway and the extrinsic, death receptor pathway. Key players in these pathways, such as the Bcl2 family proteins, cytochrome c, Apaf-1, caspases, death receptor adapter proteins, and inhibitor of apoptosis proteins, are presented from both functional and structural angles. Until only a few years ago, programmed cell death has been considered essentially synonymous with apoptosis. However, we now know that programmed cell death can also take other forms such as necrosis or necroptosis, and to this end, the mechanisms that underlie programmed necrosis in development and host defense are illustrated. The past twenty plus years have seen an incredible growth of research in cell death, with one breakthrough after another, and the legacy still goes on with constant new surprises and findings. Long live cell death!
  • 2013 Springer
    Daniel E. Johnson, editor.
    Defects in cell death pathways promote tumor development and progression, with potentially devastating consequences for cancer patients. Greater understanding of the defects occurring in cancer cells, and the unique characteristics of tumors which can make them vulnerable to cell death stimuli, offers tremendous opportunities for developing novel and effective anti-cancer therapies. In Cell Death in Cancer Biology and Treatment leading experts in the field provide a wealth of up-to-date knowledge regarding the molecular mechanisms and cell biological processes that control cell death. Each chapter also highlights recent advances in the translation of basic research findings into clinical trials. Beginning and established investigators alike will benefit from the thorough presentations of the most promising avenues for future development of cell death-based, anti-cancer strategies and agents. The volume begins with a detailed description of many of the cell death defects that have been identified in human tumor specimens. The unique bioenergetics of cancer cells, and the influence of the tumor microenvironment, autophagy, and cellular microRNAs on cancer cell death are then discussed, along with current progress in targeting these distinctive features and processes. Additional chapters describe recent advances, and the therapeutic benefits of targeting DNA repair pathways, protein chaperones, sphingolipid signaling, Bcl-2 family members, IAPs, death receptor signaling, the proteasome, and survival signaling emanating from the PI3K/AKT and RAS/RAF/MEK/ERK pathways. Finally, recent discoveries are presented regarding interactions between the immune system and dying cancer cells and the potential for optimizing these interactions to maximize anti-cancer activities. In summary, Cell Death in Cancer Biology and Treatment will be a valuable resource for scientists interested in cutting-edge understanding of aberrant cell death in cancer cells, and the multitude of innovative molecular targeting approaches that are actively being pursued to achieve selective activation of cell death in human malignancies.
  • 2011 Springer
    Thomas Dittmar, Kurt S. Zänker, editors.
    1. Introduction / Thomas Dittmar and Kurt S. Zänker -- 2. Heterochronic control of AFF-1-mediated cell-to-cell fusion in C. elegans / Lilach Friedlander-Shani and Benjamin Podbilewicz -- 3. Role of SNAREs in membrane fusion / Bhanu P. Jena -- 4. Molecular and cellular mechanisms of mammalian cell fusion / Xiaofeng Zhou and Jeffrey L. Platt -- 5. Membrane fusions during mammalian fertilization / Bart M. Gadella and Janice P. Evans -- 6. Trophoblast fusion / Berthold Huppertz and Martin Gauster -- 7. Macrophage fusion and multinucleated giant cells of inflammation / Amy K. McNally and James M. Anderson -- 8. Molecular mechanisms of myoblast fusion across species / Adriana Simionescu and Grace K. Pavlath -- 9. Cell-fusion-mediated reprogramming : pluripotency or transdifferentiation? : implications for regenerative medicine / Daniela Sanges, Frederic Lluis, and Maria Pia Cosma -- 10. Cell fusion and tissue regeneration / Manuel Álvarez-Dolado and Magdalena Martínez-Losa -- 11. Dendritic cell-tumour cell fusion vaccines / Walter T. Lee.
    Also available: Print – 2011
  • 2011 Springer
    Thomas Dittmar, Kurt S. Zänker, editors.
    Introduction / Thomas Dittmar and Kurt S. Zänker -- Horizontal Gene Transfers with or without Cell Fusions in All Categories of the Living Matter / Joseph G. Sinkovics -- Class III Viral Membrane Fusion Proteins / Marija Backovic and Theodore S. Jardetzky -- Human Trophoblast in Trisomy 21: A Model for Cell - Cell Fusion Dynamic Investigation / André Malassiné, Guillaume Pidoux, Pascale Gerbaud, Jean Louis Frendo and Danièle Evain-Brion -- Cell Fusion and Hyperactive Osteoclastogenesis in Multiple Myeloma / Franco Silvestris, Sabino Ciavarella, Sabino Strippoli and Franco Dammacco -- Cell Fusion Hypothesis of the Cancer Stem Cell / Xin Lu and Yibin Kang -- Expression of Macrophage Antigens by Tumor Cells / Ivan Shabo and Joar Svanvik -- Leukocyte-Cancer Cell Fusion: Initiator of the Warburg Effect in Malignancy? / Rossitza Lazova, Ashok Chakraborty and John M. Pawelek -- Cell Fusion, Drug Resistance and Recurrence CSCs / Christa Nagler, Kurt S. Zänker and Thomas Dittmar -- The Role of Microvesicles in Malignancies / Erna Pap.
    Also available: Print – 2011
  • 2015 Springer Protocols
    edited by Kurt Pfannkuche.
    This volume details protocols on detection and analysis of fusion events in various cell types in vitro and in vivo. Additional, protocols aim to facilitate the future highly relevant process of engineering cells for specific purposes, such as generation of transgenic embryos and development of hybrid cells, in order to tackle specific tasks in cell biology and medicine. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Cell Fusion: Overviews and Methods, Second Edition serves as an ideal reference on cell fusion and laboratory protocols.
  • 2008 Springer Protocols
    edited by Elizabeth H. Chen.
    Pt. 1. Cell fusion -- 1. Yeast mating, a model system for studying cell and nuclear fusion / Casey A. Ydenberg, Mark D. Rose -- 2. Cell fusion in the filamentous fungus, neurospora crassa / André Flei€ner, Anna R. Simonin, N. Louise Glass -- 3. Gametic cell adhesion and fusion in the uicellular alga chlamydomonas / Nedra F. Wilson -- 4. Cell Fusion in caenorhabditis elegans / Scott Alper, Benjamin Podbilewicz -- 5. Myoblast fusion in drosophila / Susan M. Abmayr, Shufei Zhuang, and Erika R. Geisbrecht -- 6. Mammalian fertilization is dependent on multiple membrane fusion events / Paul M. Wassarman, Eveline S. Litscher -- 7. Molecular control of mammalian myoblast fusion / Katie M. Jansen, Grace K. Pavlath -- 8. Placenta trophoblast fusion / Berthold Huppertz, Marcus Borges -- 9. Macrophage fusion / Agnès Vignery -- Pt. 2. Cell fusion assays -- 10. Cell fusion assays for yeast mating pairs / Eric Grote -- 11. Ultrastructural analysis of cell fusion in yeast / Alison E. Gammie -- 12. Isolation and in vitro binding of mating type plus fertilization tubules from chlamydomonas / Nedra F. Wilson -- 13. Optical imaging of cell fusion and fusion proteins in caenorhabditis elegans / Star Ems, William A. Mohler -- 14. Ultrastructural imaging of cell fusion in caenorhabditis elegans / Star Ems, William A. Mohler -- 15. Live imaging of drosophila myoblast fusion / Brian E. Richardson, Karen Beckett, Mary K. Baylies -- 16. Ultrastructural Analysis of myoblast fusion in drosophila / Shiliang Zhang, Elizabeth H. Chen -- 17. Genomic approach to myoblast fusion in drosophila / Beatriz Estrada, Alan M. Michelson -- 18. Mesenchymal cell fusion in the sea urchin embryo / Paul G. Hodor, Charles A. Ettensohn -- 19. Sperm-egg fusion assay in mammals / Naokazu Inoue, Masaru Okabe -- 20. Quantitative assays for cell fusion / Jessica H. Shinn-Thomas, Victoria L. Scranton, William A. Mohler -- 21. Fusion assays and models for the trophoblast / Sascha Drewlo, ... [et al.] -- 22. Methods to fuse macrophages in vitro / Agnès Vignery.
  • 2011 Springer
    Lars-Inge Larsson, editor.
  • 2012 Springer Protocols
    edited by Douglas J. Taatjes, Jürgen Roth.
    Digital images are data : and should be treated as such / Douglas W. Cromey -- Epi-fluorescence microscopy / Donna J. Webb and Claire M. Brown -- Live-cell migration and adhesion turnover assays / J. Lacoste, K. Young, and Claire M. Brown -- Multifluorescence confocal microscopy : application for a quantitative analysis of hemostatic proteins in human venous valves / Winifred E. Trotman, Douglas J. Taatjes, and Edwin G. Bovill -- Colocalization analysis in fluorescence microscopy / Jeremy Adler and Ingela Parmryd -- A time-lapse imaging assay to study nuclear envelope breakdown / Sunita S. Shankaran, Douglas R. Mackay, and Katharine S. Ullman -- Light sheet microsocopy in cell biology / Raju Tomer, Khaled Khairy, and Philipp J. Keller -- Image-based high-throughput screening for inhibitors of angiogenesis / Lasse Evensen, Wolfgang Link, and James B. Lorens -- Intravital microscopy to image membrane trafficking in live rats / Andrius Masedunskas [and others] -- Imaging non-fluorescent nanoparticles in living cells with wavelength-dependent differential interference contrast microscopy and planar illumination microscopy / Wei Sun, Lehui Xiao, and Ning Fang -- Laser scanning cytometry : principles and applications-an update / Piotr Pozarowski, Elena Holden, and Zbigniew Darzynkiewicz -- Laser capture microdissection for protein and NanoString RNA analysis / Yelena Golubeva [and others] -- Viewing dynamic interactons of proteins and a model lipid membrane with atomic force microscopy / Anthony S. Quinn [and others] -- Mica functionaliztion for imaging of DNA and protein-DNA complexes with atomic force microscopy / Luda S. Shlyakhtenko, Alexander A. Gall, and Yuri Lyubchenko -- Measuring the elastic properties of living cells with atomic force microscopy indentation / Joanna L. MacKay and Sanjay Kumar -- Atomic force microscopy functional imaging on vascular endothelial cells / Lilia A. Chtcheglova and Peter Hinterdorfer -- Porosome : the secretory nanomachine in cells / Bhanu P. Jena -- Stereology and morphometry of lung tissue / Christian Mühlfeld, Lars Knudsen, and Matthias Ochs -- A novel combined imaging/morphometrical method for the analysis of human sural nerve biopsies for clinical diagnosis / Michele A. vonTurkovich [and others] -- Correlative light-electron microscopy as a tool to study in vivo dynamics and ultrastructure of intracellular structures / Elena V. Polishchuk, Roman S. Polishchuk, and Alberto Luini -- Photooxidation technology for correlative light and electron microscopy / Claudia Meisslitzer-Ruppitsch [and others] -- Electron microscopy of endocytic pathways / Carmen Ranftler [and others] -- Morphological analysis of autophagy / Keisuke Tabata [and others] -- Cytochemical detection of peroxisomes and mitochondria / Nina A. Bonekamp [and others] -- Histochemical detection of lipid droplets in cultured cells / Michitaka Suzuki, Yuki Shinohara, and Toyoshi Fujimoto -- Environmental scanning electron microscopy in cell biology / J.E. McGregor [and others] -- Environmental scanning electron microscopy gold immunolabeling in cell biology / Francesco Rosso, Ferdinando Papale, and Alfonso Barbarisi -- High-pressure freezing for scanning transmission electron tomography analysis of cellular organelles / Paul Walther, Eberhard Schmid, and Katharina Höhn -- MALDI imaging mass spectrometry for direct tissue analysis / Stephan Meding and Axel Walch.
  • 2007 ScienceDirect
    edited by Yu-Li Wang, Dennis E. Discher.
    Also available: Print – 2007
  • 2011 Springer Protocols
    edited by Claire M. Wells, Maddy Parsons.
    Cell migration : an overview / Miguel Vicente-Manzanares and Alan Rick Horwitz -- Scratch-wound assay / Giles Cory -- HGF-induced DU145 cell scatter assay / Sally T. Fram, Claire M. Wells, and Gareth E. Jones -- Using the dunn chemotaxis chamber to analyze primary cell migration in real time / Sanjay Chaubey, Anne J. Ridley, and Claire M. Wells -- Imaging cells within 3D cell-derived matrix / Samantha J. King and Maddy Parsons -- Live cell imaging of neuronal growth cone motility and guidance in vitro / Daniel M. Suter -- Boyden chamber / Marco Falasca, Claudio Raimondi, and Tania Maffucci -- Transwell® invasion assays / John Marshall -- Imaging podosome dynamics and matrix degradation / Taylor W. Starnes, Christa L. Cortesio, and Anna Huttenlocher -- Endothelial cell migration under flow / Beata Wojciak-Stothard -- In vitro analysis of chemotactic leukocyte migration in 3D environments / Michael Sixt and Tim Lammermann -- Quantification of transendothelial migration using three-dimensional confocal microscopy / Robert J. Cain, Barbara Borda d'Agua, and Anne J. Ridley -- Chemotaxis of slow migrating mammalian cells analysed by video microscopy / Roman Zantl and Elias Horn -- Live cell fluorescence microscopy techniques / Shawn A. Galdeen and Alison J. North -- Measuring invasion in an organotypic model / Veronika Jenei, Maria L. Nystrom, and Gareth J. Thomas -- Analysis of cell migration using Caenorhabditis elegans as a model system / Ming-Ching Wong, Maria Martynovsky, and Jean E. Schwarzbauer -- Drosophila hemocyte migration : an in vivo assay for directional cell migration / Carolina G.A. Moreira [and others] -- Measuring inflammatory cell migration in the zebrafish / Philip M. Elks, Catherine A. Loynes, and Stephen A. Renshaw -- Border cell migration : a model system for live imaging and genetic analysis of collective cell movement / Mohit Prasad [and others] -- Assessment of development and chemotaxis in Dictyostelium discoideum mutants / Yulia Artemenko, Kristen F. Swaney, and Peter N. Devreotes -- Simple experimental and spontaneous metastasis assays in mice / Gary M. Box and Suzanne A. Eccles -- Two-photon intravital multicolour imaging to study metastatic behaviour of cancer cells in vivo / Sylvia E. Le Devedec [and others] -- Measuring angiogenesis in mice / Bernardo Tavora [and others] -- Time-lapse imaging of chick cardiac precursor cells / Junfang Song, Qiaoyun Yue, and Andrea Munsterberg -- Characterizing system performance in total internal reflection fluorescence microscopy / Juliane P. Schwarz, Ireen Konig, and Kurt I. Anderson -- Fluorescence recovery after photobleaching / Alex Carisey [and others] -- Measuring FRET using time-resolved FLIM / Penny E. Morton and Maddy Parsons -- Cell migration in confinement : a micro-channel-based assay / Melina L. Heuze [and others] -- Functional screening with a live cell imaging-based random cell migration assay / Wies van Roosmalen [and others] -- Beads on the run : beads as alternative tools for chemotaxis assays / Eric Theveneau and Roberto Mayor.
  • 2010 Karger
    volume editors, Frank Entschladen, Kurt S. Zänker.
    The migrating cell / Entschladen, F., Zänker, K.S. -- Stem cell migration in health and disease / Dittmar, T. ... [et al.] -- Leukocyte motility and human disease / Cooper, K., Nuzzi, P., Huttenlocher, A. -- Coordination of leukocyte polarity and migration / Martín-Cófreces, N.B., Serrador, J.M., Sánchez-Madrid, F. -- Positioning phosphoinositide 3-kinase in chemokine and antigen-dependent T-lymphocyte navigation mechanisms / Ward, S.G. -- Migration of functionally specialized T-helper cells : TFH cells, Th17 cells, and FoxP3+ T cells / Kim, C.H. -- ADAMs and ectodomain proteolytic shedding in leukocyte and tumour cell migration / Ager, A., Knäuper, V., Poghosyan, Z. -- Guided tour of cell migration : signals and pathways / Ratke, J., Lang, K. -- Regulation of the E-cadherin adhesion complex in tumor cell migration and invasion / Menke, A., Giehl, K. -- The cytoskeletal connection : understanding adaptor proteins / Ziegler, W.H. -- Locomotor force generation by myosins / Jbireal, J.M.A., Entschladen, F., Zänker, K.S.
  • 2006 Springer
    edited by Alan Wells.
    Cancer Morbidity and mortality result from invasive and metastatic spread. Advances in the field have highlighted the central role of cell motility during the dynamic and transient process of tumor invasion and metastasis. This book presents an overview of these studies.
  • 2012 ScienceDirect
    [edited by] Nicholas Sperelakis.
    Sect. I. Biophysical chemistry, metabolism, second messengers, and ultrastructure. Biophysical chemistry of physiological solutions / Jeffrey c. Freedman -- Physiological structure and function of proteins / Matthew R. Pincus -- Cell membranes / Jeffrey C. Freedman -- Ionophores in planar lipid bilayers / Jeffrey C. Freedman -- Cell structure / Michael S. Forbes -- Signal transduction and second messengers / Aldebaran M. Hofer -- Calcium as an intracellular second messenger: mediation by calcium-binding proteins / John R. Dedman and Marcia A. Kaetzel -- Sect. II. Membrane potential, transport physiology, pumps, and exchangers. Diffusion and permeability / Nicholas Sperelakis and Jeffrey C. Freedman -- Origin of resting membrane potentials / Nicholas Sperelakis -- Gibbs-Donnan equilibrium potentials / Nicholas Sperelakis -- Mechanisms of carrier-mediated transport: facilitated diffusion, cotransport and countertransport / Steven M. Grassl -- Active ion transport by ATP-driven ion pumps / Robert A. Farley -- Ca2+ -ATPases / Tracy J. Pritchard, Istvan Edes and Evangelia G. Kranias -- Na+ -Ca2+ exchange currents / John H.B. Bridge, Natalia S. Torres and Michela Ottolia -- Intracellular chloride regulation / Francisco J. Alvarez-Leefmans -- Osmosis and regulation of cell volume / Clive M. Baumgarten and Joseph J. Feher -- Intracellular pH regulation / Robert W. Putnam -- Sect. III. Membrane excitability and ion channels. Cable properties and propagation of action potentials / Nicholas sperelakis -- Electrogenesis of membrane excitability / Nicholas Sperelakis -- Patch-clamp techniques / Laura Conforti -- Structure and mechanism of voltage-gated ion channels / Simon Rock Levinson and William A. Sather -- Biology of gap junctions / Richard D. Veenstra -- Regulation of cardiac ion channels by cyclic nucleotide-dependent phosphorylation / Gordon M. Wahler and Nicholas Sperelakis -- Direct regulation of ion channels by GTP-binding proteins / Atsushi Inanobe and Yoshihisa Kurachi -- Developmental changes in ion channels / Takeshi Kobayashi ... [et al.] -- Regulation of ion channel localization and activity through interactions with the cytoskeleton / Stephen Lambert -- Why are so many ion channels mechanosensitives? / Catherine E. Morris -- Sect. IV. Ion channels as targets for toxins, drugs, and genetic diseases. Ion channels as targets for toxins / Kenneth M. Blumenthal -- Ion channels as targets for drugs / Seth Robey, Kevin J. Sampson and Robert S. Kass -- Inherited diseases of ion transport / Robert A. Farley -- Sect. V. Synaptic transmission and sensory transduction. Ligand-gated ion channels / Kenneth R. Tovar and Gary L. Westbrook -- Synaptic transmission / Janusz B. Suszkiw -- Excitation-secretion coupling / Nicole Gallo-Payet and Marcel Daniel Payet -- Stimulus-response coupling in metabolic sensor cells / Stan Misler -- Cyclic nucleotide-gated ion channels / Anita L. Zimmerman -- Sensory receptors and mechanotransduction / Andrew S. French and Päivi H. Torkkeli -- Acoustic transduction / Daniel C. Marcus -- Visual transduction / Anita L. Zimmerman -- Gustatory and olfactory sensory transduction / Stephen D. Roper -- Infrared sensory organs / Stephen D. Roper and Michael S. Grace -- Electroreceptors and magnetoreceptors / Timothy C. Tricas and Bruce A. Carlson -- Sect. VI. Muscle and other contractile systems. Skeletal muscle excitability / Nicholas Sperelakis, Judith Heiny and Hugo gonzalez-Serratos -- Cardiac action potentials / Gordon M. Wahler -- Smooth muscle excitability / Neil D. Detweiler ... [et al.] -- Excitation-contraction coupling in skeletal muscle / Judith A. Heiny and Gerhard Meissner -- Contraction of muscles: mechanochemistry / Richard J. Paul -- Flagella, cilia, actin- and centrin-based movement / Kenneth W. Foster -- Electrocytes of electric fish / Anthony L. Gotter, Marcia A. Kaetzel and John R. Dedman -- Sect. VII. Protozoa and bacteria. Physiological adaptations of protists / Michael Levandowsky -- Physiology of prokaryotic cells / Dennis W. Grogan -- Sect. VIII. Specialized processes: photosynthesis and bioluminescence. Photosynthesis / Darrell Fleischman -- bioluminescence / J. Woodland Hastings.
  • 2013 Springer Protocols
    edited by Lorenzo Galluzzi [and others].
    Cell senescence as both a dynamic and a static phenotype / Andrew R.J. Young, Masako Narita, and Masashi Narita -- Senescence regulation by mTOR / Vjekoslav Dulic -- Senescence regulation by the p53 protein family / Yingjuan Qian and Xinbin Chen -- Markers of cellular senescence / Amancio Carnero -- Biomarkers of cell senescence assessed by imaging cytometry / Hong Zhao and Zbigniew Darzynkiewicz -- Cytofluorometric assessment of cell cycle progression / Ilio Vitale, Mohamed Jemaà, Lorenzo Galluzzi, Didier Metivier, Maria Castedo, and Guido Kroemer -- Quantification of cell cycle-arresting proteins / Oliver Kepp, Isabelle Martins, Laurie Menger, Mickaël Michaud, Sandy Adjemian, Abdul Qader Sukkurwala, Lorenzo Galluzzi, and Guido Kroemer -- Colorimetric detection of senescence-associated [beta] galactosidase / Koji Itahana, Yoko Itahana, and Goberdhan P. Dimri -- Chemiluminescent detection of senescence-associated [beta] galactosidase / Vinicius Bassaneze, Ayumi Aurea Miyakawa, and José Eduardo Krieger -- Detection of the senescence-associated secretory phenotype (SASP) / Francis Rodier -- Unbiased characterization of the senescence-associated secretome using SILAC-based quantitative proteomics / Juan Carlos Acosta, Ambrosius P. Snijders, and Jesús Gil -- Detection of senescence-associated heterochromatin foci (SAHF) / Katherine M. Aird and Rugang Zhang -- Monitoring DNA damage during cell senescence / Glyn Nelson and Thomas von Zglinicki -- Assessment and quantification of telomerase enzyme activity / Michelle F. Maritz, Laura A. Richards, and Karen L. MacKenzie -- Methods for the assessment of telomere status / Asako J. Nakamura -- Detection of nuclear envelope alterations in senescence / Clea Bárcena, Fernando G. Osorio, and José Maria Pérez Freije -- Measuring reactive oxygen species in senescent cells / João F. Passos, Satomi Miwa, and Thomas von Zglinicki -- Quantification of protein carbonylation / Nancy B. Wehr and Rodney L. Levine -- Assays for the measurement of lipid peroxidation / Ana Cipak Gasparovic, Morana Jaganjac, Branka Mihaljevic, Suzana Borovic Sunjic, and Neven Zarkovic -- Raman spectroscopy for the detection of AGEs/ALEs / J. Renwick Beattie, John J. McGarvey, and Alan W. Stitt -- Monitoring oncogenic B-RAF-induced senescence in melanocytes / Sieu L. Tran and Helen Rizos -- Methods to investigate the role of SIRT1 in endothelial senescence / Bo Bai and Yu Wang -- Monitoring nutrient signaling through the longevity protein p66SHC1 / Sofia Chiatamone Ranieri and Giovambattista Pani -- Profiling the metabolic signature of senescence / Florian M. Geier, Silke Fuchs, Gabriel Valbuena, Armand M. Leroi, and Jacob G. Bundy -- Genome-wide RNAi screening to identify regulators of oncogene-induced cellular senescence / Narendra Wajapeyee, Sara K. Deibler, and Michael R. Green -- Integrated approach for monitoring cell senescence / Tatiana V. Pospelova, Zhanna V. Chitikova, and Valery A. Pospelov -- Robust multiparametric assessment of cellular senescence / Clara Correia-Melo, Diana Jurk, and João F. Passos -- Assessing chronological aging in bacteria / Stavros Gonidakis and Valter D. Longo -- Assessing organismal aging in the filamentous fungus Podospora anserina / Heinz D. Osiewacz, Andrea Hamann, and Sandra Zintel -- Assessing chronological aging in Saccharomyces cerevisiae / Jia Hu, Min Wei, Mario G. Mirisola, and Valter D. Longo -- Assessing aging and senescent decline in Caenorhabditis elegans : cohort survival analysis / Eirini Lionaki and Nektarios Tavernarakis -- High-throughput and longitudinal analysis of aging and senescent decline in Caenorhabditis elegans / Eirini Lionaki and Nektarios Tavernarakis -- Assessing senescence in Drosophila using video tracking / Reza Ardekani, Simon Tavaré, and John Tower -- Assessing vascular senescence in zebrafish / Sandra Donnini, Antonio Giachetti, and Marina Ziche.
  • 2015 Springer
    Henry J. Leese, Daniel R. Brison, editors.
    The book considers signaling events from the zygote embryo through to the blastocyst with relevant data from embryonic stem (ES) cells, including dialogue with the extracellular environment and with the maternal tract during the implantation process. Application of the knowledge described to improve the success of human and animal assisted conception is considered where appropriate, but the focus is largely on fundamental rather than applied cell/molecular biology, as this is the area that has historically been neglected. While the general features of metabolism during preimplantation development are well established, especially in terms of nutrient requirements, uptake and fate, remarkably little is known about early embryo signaling events, intracellular or intercellular, between individual embryos in vitro or with the female reproductive tract in vivo. This contrasts with the wealth of information on cell signaling in somatic cells and tissues, as a glance at any textbook of biochemistry illustrates. This lack of information is such that our understanding of the molecular cell biology of early embryos -- a prerequisite to defining the mechanisms which regulate development at this critical stage of the life cycle -- is seriously incomplete. This volume is the first to address this issue by describing the current state of knowledge on cell signaling during mammalian early embryo development and highlighting priority areas for research.
    Also available: Print – 2015
  • 2005 Springer
    edited by Jahar Bhattacharya.
    Heme oxygenase-1 and carbon monoxide in vascular regulation / Stefan W. Ryter and Augustine M.K. Choi -- Tumor necrosis factor-[alpha]/receptor signaling through the Akt kinase / Osman Nidai Ozes ... [et al.] -- Protein modifications by nitric oxide and reactive nitrogen species / Harry Ischiropoulos -- Redox signaling in hypoxic pulmonary vasoconstriction / E. Kenneth Weir ... [et al.] -- cGMP-dependent protein kinase in regulation of the perinatal pulmonary circulation / Usha Raj ... [et al.] -- Glutamate receptor activation in the pathogenesis of acute leg injury / Sami I. Said -- Carboxyl methylation of small GTPases and endothelial cell function / Sharon Rounds, Elizabeth O. Harrington, and Qing Lu -- Pressure-induced inflammatory signaling in lung endothelial cells / Wolfgang M. Kuebler -- Regulation of endothelial barrier function: contributions of the transcellular and paracellular pathways / Dolly Mehta, Richard D. Minshall, and Asrar B. Malik -- Sphingolipid signaling: implications for vascular biology / Margaret M. Harnett -- Regulations of cytokine signaling / Bao Q. Vuong ... [et al.] -- Cell signaling by vasoactive agents / Barry L. Fanburg ... [et al.] -- Reactive oxygen species and cell signaling in lung ischemia / Aron B. Fisher -- Pulmonary vascular barrier regulation by thrombin and Edg receptors / Jeffrey R. Jacobson and Joe G.N. Garcia -- Second-messenger signaling in lung capillaries / Kaushik Parthasarathi -- Plasma membrane-to-nucleus calcium signaling / Giles E. Hardingham -- Signaling by mitochondria / Navdeep S. Chandel -- Pro-inflammatory signaling by endothelial focal complexes in lung / Sunita Bhattacharya -- Hydrogen peroxide as intracellular messenger: production, target, and elimination / Sue Goo Rhee ... [et al.] -- Calcium-inhibited adenylyl cyclase (AC6) controls endothelial cell barrier function / Troy Stevens.
  • 2011 Springer
    Yasushi Sako, Masahiro Ueda, editors.
  • 2009 Springer
    Adrian Gee, editor.
    Part 1. Regulatory. -- Regulation of Cell Product Manufacturing and Delivery: A United States Perspective / R.W. Lindblad -- The Regulatory Situation for Academic Cell Therapy Facilities in Europe / I. Slaper-Cortenbach, M. Scott, D. Herrmann, M. Introna and K. Theunissen, et al. -- A Regulatory System for Cell and Tissue Therapies: Proposed Approach in Australia / A. Farrugia -- Part 2. GMP Facility Design. -- University of Minnesota - Molecular and Cellular Therapeutics (MCT) / D.H. McKenna -- University of Pittsburgh Cancer Institute - Hematopoietic Stem Cell Laboratory (HSC Lab)/Immunological Monitoring and Cellular Products Laboratory (IMCPL) / D.L. Griffin, A.D. Donnenberg and T.L. Whiteside / Baylor College of Medicine - Center for Cell and Gene Therapy (CAGT) / A. Gee -- Design of a New GMP Facility - Lessons Learned / A. Gee -- Part 3. Professional Cell Therapy Standards. -- AABB Cell Therapy Standards / Z.M. Szczepiorkowski and E. Nunes -- Professional Standards for Cellular Therapies: Foundation for the Accreditation of Cellular Therapy (FACT) / P.I. Warkentin -- Part 4. Facility Operations -- Standard Operating Procedures / C.G. Lindgren -- Staffing, Training, and Competency / D.M. Kadidlo -- Cleaning Procedures / A. Gee and D.L. Lyon -- Environmental Monitoring / A. Gee and D.L. Lyon -- Supply Management / A. Gee and C.M. Rooney -- Facility Equipment / D.L. Griffin -- Quality / J.W. Atkins -- Product Manufacturing / A. Gee -- Product Review, Release, and Administration / N.H. Collins -- Use of a Facility Master File to Facilitate Regulatory Submissions for Cell Therapy Products / E.J. Read and H.M. Khuu.
  • 2006 Springer
    edited by Cyndy Davis Sanberg and Paul R. Sanberg.
    Current views of the embryonic and neural stem cell : cell replacement or molecular repair? / Roya Sabetrasekh ... [et al.] -- Developing novel cell sources for transplantation in Parkinson's disease / Nicolaj S. Christophersen ... [et al.] -- Neural transplantation in the nonhuman primate model of Parkinson's disease / Kimberly B. Bjugstad and John R. Sladek, Jr. -- Cell-based therapy for Huntington's disease / Claire M. Kelly, Stephen B. Dunnett, and Anne E. Rosser -- Use of bone marrow stem cells as therapy for behavioral deficits in rodent models of Huntington's disease / Gary L. Dunbar, Justin D. Oh-Lee, and Laurent Lescaudron -- Human neuroteratocarcinoma cells as a neural progenitor graft source for cell transplantation in stroke / Cesario V. Borlongan ... [et al.] -- Therapeutic applications of bone marrow-derived stem cells in neurologic injury and disease / C. Dirk Keene ... [et al.] -- Cell therapy for models of pain and traumatic brain injury / Mary Eaton and Jacqueline Sagen -- The use of sertoli cells in neural transplantation / Dwaine F. Emerich, Cesario V. Borlongan, and Craig Halberstadt -- The choroids plexus : a novel graft source for neural transplantation / Cesario V. Borlongan ... [et al.] -- Progress and challenges in immunoisolation for CNS cell therapy / Christopher G. Thanos and Dwaine F. Emerich -- Evidence-based methodology for advancing neural reconstruction / Stephen Polgar -- Hematopoietic cell therapy for brain repair / Martina Vendrame and Alison E. Willing -- Stem cells and regenerative medicine : commercial and pharmaceutical implications / L. Eduardo Cruz and Silvia P. Azevedo.
    Also available: Print – 2006
  • 2013 Springer
    Jukka Jolkkonen, Piotr Walczak, editors.
    Stroke remains one of the main causes of death and disability worldwide. Effective therapy for stroke recovery remains an unmet need. Much hope and promises are placed on cell-based therapies. The aim of this book is to provide focused yet comprehensive reviews on the current state of cell-based approaches in the treatment of stroke. The topics covered include experimental data on functional outcome after intravascular and intracerebral delivery of cells in stroke animals, followed by translational chapters which will rely heavily on the use of different imaging modalities in the tracking of cells. The last and most challenging part will describe the early phase of clinical studies, providing guidelines for future research and clinical applications. Clearly and concisely written, this text will be a useful resource for neurologists, radiologists, and neuroscientists interested in cell transplantation as a therapeutic strategy for stroke patients.
  • 2014 Karger
    volume editors, Ricardo P. Casaroli-Marano, Marco A. Zarbin.
    Age-related macular degeneration: clinical findings, histopathology, and imaging techniques / Zarbin, M.A.; Casaroli-Marano, R.P.; Rosenfeld, P.J. -- General pathophysiology in retinal degeneration / Wert, K.J.; Lin, J.H.; Tsang, S.H. -- Juvenile-onset macular degeneration and allied disorders / North, V.; Gelman, R.; Tsang, S.H. -- Diagnosis and complementary examinations / Menghini, M.; Duncan, J.L. -- Stem cells for retinal repair / Stern, J.; Temple, S. -- Differentiation of pluripotent stem cells into retinal pigmented epithelium / Croze, R.H.; Clegg, D.O. -- Neural retinal regeneration with pluripotent stem cells / Ramsden, C.M.; Powner, M.B.; Carr, A.-J.F.; Smart, M.J.K.; da Cruz, L.; Coffey, P.J. -- Stem cells, retinal ganglion cells and glaucoma / Sluch, V.M.; Zack, D.J. -- Stem cells : immunology and immunomodulation / Tena, A.; Sachs, D.H. -- Biochemical restoration of aged human Bruch's membrane : experimental studies to improve retinal pigment epithelium transplant survival and differentiation / Sugino, I.K.; Sun, Q.; Cheewatrakoolpong, N.; Malcuit, C.; Zarbin, M.A. -- Approaches to cell delivery : substrates and scaffolds for cell therapy / Kundu, J.; Michaelson, A.; Baranov, P.; Young, M.J.; Carrier, R.L. -- Microdevice-based cell therapy for age-related macular degeneration / Lu, B.; Tai, Y.-C.; Humayun, M.S. -- Cell and gene therapy / Rao, R.C.; Zacks, D.N. -- Cellular manufacturing for clinical applications / Sheu, J.; Klassen, H.; Bauer, G. -- Regulatory issues in cell-based therapy for clinical purposes / Casaroli-Marano, R.P.; Tabera, J.; Vilarrodona, A.; Trias, E.
  • 2006. Springer
    [edited by] Frantisek Baluska, Dieter Volkmann, Peter W. Barlow.
    Cell-cell channels and their implications for cell theory / Frantisek Baluska, Dieter Volkmann, and Peter W. Barlow -- Mating cell-cell channels in conjugating bacteria / Elisabeth Grohmann -- The tetrahymena conjugation junction / Eric S. Cole -- Cytoplasmic bridges in volvox and its relatives / Harold J. Hoops, Ichiro Nishii, and David L. Kirk -- Vegetative hyphal fusion in filamentous fungi / Nick D. Read and M. Gabriela Roca -- Plasmodesmata: cell-cell channels in plants / Yoselin Benitez Alfonso, Laurence Cantrill, and David Jackson -- Sieve-pore plugging mechanisms / Aart J.E. van Bel -- Actin and myosin VIII in plant cell-cell channels / Jozef Samaj ... [et al.] -- Cell-cell communication in wood / John R. Barnett -- TMV movement protein targets cell-cell channels in plants and prokaryotes: possible roles of tubulin- and FtsZ-based cytoskeletons / Manfred Heinlein -- Viral movement proteins induce tubule formation in plant and insect cells / Jan W.M. van Lent and Corinne Schmitt-Keichinger -- Cell-cell movements of transcription factors in plants / Alexis Maizel -- Gap junctions: cell-cell channels in animals / Fabio Mammano -- Tunneling nanotubes: membranous channels between animal cells / Hans-Hermann Gerdes and Amin Rustom -- Cytoplasmic bridges as cell-cell channels of germ cells / Sami Ventelä -- Fusome as a cell-cell communication channel of drosophila ovarian cyst / Jean-René Huynh -- Cytonemes as cell-cell channels in human blood cells / Svetlana Ivanovna Galkina .. [et al.] -- Paracellular pores in endothelial barriers / Luca Manzi and Gianfranco Bazzoni -- Channels across endothelial cells / Radu V. Stan -- Molecular transfers through transient lymphoid cell-cell channels / Mary Poupot, Julie Gertner, and Jean-Jacques Fournié -- Cell-cell transport of homeoproteins: with or without channels / Alain Joliot and Alain Prochiantz -- Virological synapse for cell-cell spread of viruses / Eduardo Garcia and Vincent Piguet -- Cell-cell fusion: transient channels leading to plasma membrane merger / William A. Mohler.
  • 2013 Springer Protocols
    edited by Troy A. Baudino.
    Proteomics analysis of contact-initiated eph receptor-ephrin signaling / Claus Jorgensen and Alexei Poliakov -- Control of vascular tube morphogenesis and maturation in 3D extracellular matrices by endothelial cells and pericytes / George E. Davis ... [and 8 others] -- Analyzing cell-cell interactions in 3-dimensional adhesion assays / Stephanie L.K. Bowers and Troy A. Baudino -- Production of spontaneously beating neonatal rat heart tissue for calcium and contractile studies / Fnu Gerilechaogetu ... [and 6 others] -- Paracrine communication between mechanically stretched myocytes and fibroblasts / Hao Feng ... [and 6 others] -- Assessing blood-brain barrier function using in vitro assays / Joseph Bressler, Katherine Clark, and Cliona O'Driscoll -- Methods to assess tissue permeability / Juan C. Ibla and Joseph Khoury -- In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature / F. Bartsch ... [and 4 others] -- Cell membrane vesicles as a tool for the study of direct epithelial-stromal interaction: lessons from CD147 / Eric Gabison ... [and 3 others] -- Microencapsulation of stem cells to study cellular interactions / Keith Moore, Adam Vandergriff, and Jay D. Potts -- Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies / Timothy N. Feinstein -- Single cell analysis of lipid rafts / William T. Lee -- Micropatterning cell adhesion on polyacrylamide hydrogels / Jian Zhang ... [and 3 others] -- Measuring cell-cell tugging forces using bowtie-patterned mPADs (microarray post detectors) / Daniel M. Cohen, Mike T. Yang, and Christopher S. Chen -- Generation and analysis of biosensors to measure mechanical forces within cells / Katharina Austen ... [and 4 others] -- Proteomic analysis of the left ventricle post-myocardial infarction to identify in vivo candidate matrix metalloproteinase substrates / Andriy Yabluchanskiy ... [and 5 others].
  • 2005 Springer
    Agata Copani, Ferdinando Nicoletti and I.N.M. Neuromed.
    Cell cycle activation in neurons: the final exit of brain-morpho-dysregulation / Thomas Arendt -- Paved with good intentions: the link between cell cycle and cell death in the mammalian central nervous system / Yan Yang and Karl Herrup -- The role of presenilins in the cell cycle and apoptosis / Mervyn J. Monteiro -- Cell cycle activation and cell death in the nervous system / Zsuzsanna Nagy -- Cell cycle and chromosome segregation defects in Alzheimer's disease / Huntington Potter -- Alzheimer disease-a new beginning or a final exit? / Mark E. Obrenovich ... [et al.] -- From cell cycle activation to the inhibition of the WNT pathway: a hypothesis of how neurons die in response to B-Amyloid / Agata Copani ... [et al.].
  • 2010 Springer
    Roy Zent, Ambra Pozzi, editors.
  • 2015 Springer Protocols
    edited by Ülo Langel.
    Classes of cell-penetrating peptides / Margus Pooga and Ülo Langel -- Penetratin story : an overview / Edmond Dupont, Alain Prochiantz, and Alain Joliot -- Prediction of cell-penetrating peptides / Mattias Hällbrink and Mati Karelson -- Computer-aided virtual screening and designing of cell- penetrating peptides / Ankur Gautam, Kumardeep Chaudhary, Rahul Kumar, and Gajendra Pal Singh Raghava -- Investigating membrane interactions and structures of CPPs / Fatemeh Madani and Astrid Gräslund -- Determining the effects of membrane-interacting peptides on membrane integrity / William C. Wimley -- Study of CPP mechanisms by mass spectrometry / Sandrine Sagan, Chérine Bechara, and Fabienne Burlina -- Methods to study the role of the glycocalyx in the uptake of cell-penetrating peptides / Samuel Schmidt, Rike Wallbrecher, Toin H. van Kuppevelt, and Roland Brock -- Toxicity, immunogenicity, uptake, and kinetics methods for CPPs / Julia Uusna, Kent Langel, and Ülo Langel -- Unraveling the mechanisms of peptide-mediated delivery of nucleic acids using electron microscopy / Helerin Margus, Carmen Juks, and Margus Pooga -- SCARA involvement in the uptake of nanoparticles formed by cell-penetrating peptides / Henrik Helmfors, Staffan Lindberg, and Ülo Langel -- Protein mimicry and the design of bioactive Cell- penetrating peptides / John Howl and Sarah Jones -- Pepducins and other lipidated peptides as mechanistic probes and therapeutics / Ping Zhang, Lidija Covic, and Athan Kuliopulos -- Identification and characterization of homing peptides using in vivo peptide phage display / Maija Hyvönen and Pirjo Laakkonen -- Antimicrobial and antiviral applications of cell- penetrating peptides / Kalle Pärn, Elo Eriste, and Ülo Langel -- Visualizing actin architectures in cells incubated with cell-penetrating peptides / Lin He, Peter D. Watson, and Arwyn T. Jones -- Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro / Stine Rønholt, Mie Kristensen, and Hanne Mørck Nielsen -- Pathway toward tumor cell-selective CPPs? / Isabel D. Alves, Manon Carré, and Solange Lavielle -- PepFects and nickFects for the intracellular delivery of nucleic acids / Piret Arukuusk, Ly Pärnaste, Mattias Hällbrink, and Ülo Langel -- In vitro assays to assess exon skipping in duchenne muscular dystrophy / Prisca Boisguerin, Liz O'Donovan, Michael J. Gait, and Bernard Lebleu -- Applications of ApoB LDLR-binding domain approach for the development of CNS-penetrating peptides for alzheimer's disease / Eliezer Masliah and Brian Spencer -- CPP-based delivery system for in vivo gene delivery / Kaido Kurrikoff, Kadi-Liis Veiman, and Ülo Langel -- Application of CPPs for brain delivery / Artita Srimanee, Jakob Regberg, and Ülo Langel -- Intracellular delivery of nanoparticles with cell penetrating peptides / Giuseppina Salzano and Vladimir P. Torchilin -- Multifunctional oligoaminoamides for the receptor-specific delivery of therapeutic RNA / Judith Weber, Ulrich Lächelt, and Ernst Wagner -- Cell penetrating peptides for chemical biological studies / Ikuhiko Nakase, Toshihide Takeuchi, and Shiroh Futaki -- Experiences with CPP-based self assembling peptide systems for topical delivery of botulinum toxin / Jane Lee, Phil Kennedy, and Jacob M. Waugh -- Applications of CPPs in genome modulation of plants / Alicja Ziemienowicz, Jordan Pepper, and François Eudes -- DNA transfer into animal cells using stearylated CPP based Transfection Reagent / Kristiina Karro, Tiiu Männik, Andres Männik, and Mart Ustav -- Live cell genomics : cell-specific transcriptome capture in live tissues and cells / Thomas J. Bell and James Eberwine -- Live cell genomics : RNA exon-specific RNA-binding protein isolation / Thomas J. Bell and James Eberwine.
  • v. 2, 1970.
    [By] Edmund B. Sandborn. Version française par Réal Gagnon, Pierre Jean [et] Bernard Messier.
    Status: Not Checked OutLane Catalog Record
  • 2014 Springer
    Laurent Nguyen, Simon Hippenmeyer, editors.
    Cellular and Molecular Control of Neuronal Migration provides an up-to-date collection of reviews on the molecular and cellular principles of neuronal migration in the mammalian brain. Over the last decades a rich catalogue of signaling molecules controlling neuronal migration has been compiled, and within this book an international panel of experts provides up-to-date discussions of the state of knowledge how these distinct signaling pathways regulate various aspects of neuronal migration. This book introduces the reader to the latest discoveries and concepts of neuronal migration enabled through the application of most sophisticated methods and cutting edge experimental approaches. Cellular and Molecular Control of Neuronal Migration also provides an update on the underlying cellular and molecular basis of neurodevelopmental migration disorders in human patients for all interested neuroscientists and clinicians.
    Also available: Print – 2014
  • 2013 Springer Protocols
    edited by Volkmar Weissig, Tamer Elbayoumi, and Mark Olsen.
    Nanoparticle-GFP "chemical nose" sensor for cancer cell identification / Daniel F. Moyano and Vincent M. Rotello -- Method to map spatiotemporal pH changes in a multicellular living organism using a DNA nanosensor / Sunaina Surana and Yamuna Krishnan -- Simple method to visualize and assess the integrity of lysosomal membrane in mammalian cells using a fluorescent dye / Syed K. Sohaebuddin and Liping Tang -- Gold nanoparticle as a marker for precise localization of nano-objects within intracellular sub-domains / Valeriy Lukyanenko and Vadim Salnikov -- Immunoisolation of nanoparticles containing endocytic vesicles for drug quantitation / Ari Nowacek [and others] -- Methods for isolation and identification of nanoparticle-containing subcellular compartments / Ari Nowacek [and others] -- Permeabilization of cell membrane for delivery of nano-objects to cellular sub-domains / Valeriy Lukyanenko -- Method to encapsulate molecular cargo within DNA Icosahedra / Dhiraj Bhatia [and others] -- Delivery of plasmid DNA to mammalian cells using polymer-gold nanorod assemblies / James Ramos, Huang-Chiao Huang, and Kaushal Rege -- Lipophilic-formulated gold porphyrin nanoparticles for chemotherapy / Puiyan Lee and Kenneth K.Y. Wong -- Mitochondria-specific nano-emulsified therapy for myocardial protection against doxorubicin-induced cardiotoxicity / Amy Faulk, Volkmar Weissig, and Tamer Elbayoumi -- Formation of pit-spanning phospholipid bilayers on nanostructured silicon dioxide surfaces for studying biological membrane events / Indriati Pfeiffer and Michael Zach -- Characterization of nanoparticle-lipid membrane interactions using QCM-D / Rickard Frost and Sofia Svedhem -- Single-cell nanosurgery / Maxwell B. Zeigler and Daniel T. Chiu -- Single quantum dot imaging in living cells / Jerry C. Chang and Sandra J. Rosenthal -- Fabrication of fluorescent silica nanoparticles with aggregation-induced emission luminogens for cell imaging / Sijie Chen, Jacky W.Y. Lam, and Ben Zhong Tang -- Monitoring the degradation of reduction-sensitive gene carriers with fluorescence spectroscopy and flow cytometry / Constantin Hozsa, Miriam Breunig, and Achim Gopferich -- Quantification of intracellular mitochondrial displacements in response to nanomechanical forces displacements in response to nanomechanical forces / Yaron R. Silberberg and Andrew E. Pelling -- Imaging select mammalian organelles using fluorescent microscopy : application to drug delivery / Paul D.R. Dyer [and others] -- Real-time particle tracking for studying intracellular trafficking of pharmaceutical nanocarriers / Feiran Huang [and others] -- Interactions of nanoparticles with proteins : determination of equilibrium constants / Lennart Treuel and Marcelina Malissek -- Tracing the endocytic pathways and trafficking kinetics of cell signaling receptors using single QD nanoparticles / Katye M. Fichter and Tania Q. Vu -- Cellular internalization of quantum dots / Yue-Wern Huang [and others] -- Electrochemical scanning tunneling microscopy and spectroscopy for single-molecule investigation / Andrea Alessandrini and Paolo Facci -- Intracellular delivery of biologically active proteins with peptide-based carriers / Seong Loong Lo and Shu Wang -- Lipo-oligoarginine-based intracellular delivery / Jae Sam Lee and Ching-Hsuan Tung -- Fluorescent spherical monodisperse silica core-shell nanoparticles with a protein-binding biofunctional shell / Achim Weber, Marion Herz, and Gunter E.M. Tovar -- Direct quantification of PTD transduction using real-time monitoring / Mi-Sook Lee and Song Her -- Genotoxic assessment of carbon nanotubes / Olivera Neskovic [and others] -- Separation science : principles and applications for the analysis of bionanoparticles by asymmetrical flow field-flow fractionation (AF4) / Alexandre Moquin, Francoise M. Winnik, and Dusica Maysinger -- Polymersomes-mediated delivery of fluorescent probes for targeted and long-term imaging in live cell microscopy / Irene Canton and Giuseppe Battaglia -- Protocol for the preparation of stimuli-responsive gold nanoparticles capped with elastin-based pentapeptides / Vincent Lemieux, P. Hans H.M. Adams, and Jan C.M. van Hest.
  • 2007 ScienceDirect
    edited by J. Richard McIntosh.
    Physics of Rapid Cooling and Its Implications for Cryoimmobilization of Cells / J. Dubochet -- Cryopreparation Methods for Electron Microscopy of Selected Model Systems / K. McDonald -- Cryopreparation Methodology for Plant Cell Biology / M. W. Hess -- Correlative Light and Electron Microscopy of Early Caenorhabditis elegans Embryos in Mitosis / T. Muller-Reichert -- Understanding Microtubule Organizing Centers by Comparing Mutant and Wild-Type Structures with Electron Tomography / E. T. O'Toole -- Whole-Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography / J. L. Hoog -- Electron Microscopy of Archaea / C. E. Robertson -- Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography / B. J. Marsh -- Visualization of Membrane-Cytoskeletal Interactions During Plant Cytokinesis / M. S. Otegui -- Electron Tomographic Methods for Studying the Chemical Synapse / C. P. Arthur -- Using Electron Microscopy to Understand Functional Mechanisms of Chromosome Alignment on the Mitotic Spindle / B. F. McEwen -- Electron Microscopic Analysis of the Leading Edge in Migrating Cells / T. Svitkina -- Imaging Actomyosin In Situ / K. A. Taylor -- Electron Tomography of Bacterial Chemotaxis Receptor Assemblies / P. Zhang -- How to "Read" a Vitreous Section / J. Dubochet -- Single-Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction / I. I. Serysheva -- Electron Microscopy of Microtubule-Based Cytoskeletal Machinery / A. Hoenger -- Reconstructing the Endocytotic Machinery / Y. Cheng -- 3D Immunolocalization with Plastic Sections / M. K. Morphew -- Electron Microscopy Analysis of Viral Morphogenesis / A. Pelchen-Matthews -- Electron Tomography of Immunolabeled Cryosections / M. S. Ladinsky -- Visualizing Macromolecules with Fluoronanogold: From Photon Microscopy to Electron Tomography / T. Cheutin -- Markers for Correlated Light and Electron Microscopy / G. E. Sosinsky -- Localizing Specific Elements Bound to Macromolecules by EFTEM / R. D. Leapman -- Localization of Protein Complexes by Pattern Recognition / C. Best -- The Application of Energy-Filtered Electron Microscopy to Tomography of Thick, Selectively Stained Biological Samples / J. C. Bouwer -- Optimization of Image Collection for Cellular Electron Microscopy / P. Mooney -- Future Directions for Camera Systems in Electron Microscopy / N. Xuong -- Structure Determination In Situ by Averaging of Tomograms / F. Forster -- Methods for Image Segmentation in Cellular Tomography / K. Sandberg -- Database Resources for Cellular Electron Microscopy / M. E. Martone.
    Also available: Print – 2007
  • 2009 Springer
    Christian Ehnholm, editor.
  • v. 1-4, 1999-2002. Springer
  • v. 5-, 2002- Springer
  • 2010 Springer
    Jens F. Rehfeld, Jens R. Bundgaard (eds.).
    Also available: Print – 2010
  • 2012 Springer
    [edited by] Livio Luzi.
    1. Human Evolution and Physical Exercise: The Concept of Being "Born to Run" / Livio Luzi -- 2. Cell Morphology and Function: The Specificities of Muscle Cells / Anna Maestroni -- 3. The Cell Membrane of the Contractile Unit / Gianpaolo Zerbini -- 4. Gene Polymorphisms and Athletic Performance / Ileana Terruzzi -- 5. Nutrients and Whole-Body Energy Metabolism: The Impact of Physical Exercise / Stefano Benedini -- 6. Mitochondrial and Non-mitochondrial Studies of ATP Synthesis / Roberto Codella -- 7. Excessive Nutrients and Regional Energy Metabolism / Gianluca Perseghin -- 8. Muscle Biopsy To Investigate Mitochondrial Turnover / Rocco Barazzoni -- 9. Introduction to the Tracer-Based Study of Metabolism In Vivo / Andrea Caumo and Livio Luzi -- 10. Physical Activity and Inflammation / Raffaele Di Fenza and Paolo Fiorina -- 11. The HPA Axis and the Regulation of Energy Balance / Francesca Frigerio -- 12. Physical Exercise in Obesity and Anorexia Nervosa / Alberto Battezzati and Simona Bertoli -- 13. Physical Exercise and Transplantation / Valentina Delmonte, Vincenzo Lauriola, Rodolfo Alejandro and Camillo Ricordi -- 14. The Baboon as a Primate Model To Study the Physiology and Metabolic Effects of Exercise / Francesca Casiraghi, Alberto Omar Chavez, Nicholas Musi and Franco Folli.
  • 2012 ClinicalKey
    edited by Mordecai P. Blaustein, Joseph P.Y. Kao, Donald R. Matteson.
    Introduction : homeostasis and cellular physiology -- Diffusion and permeability -- Osmotic pressure and water movement -- Electrical consequences of ionic gradients -- Ion channels -- Passive electrical properties of membranes -- Generation and propagation of the action potential -- Ion channel diversity -- Electrochemical potential energy and transport processes -- Passive solute transport -- Active transport -- Synaptic physiology I -- Synaptic physiology II -- Molecular motors and the mechanism of muscle contraction -- Excitation-contraction coupling in muscle -- Mechanics of muscle contraction.
  • 2013 CRCnetBASE
    Marco Scianna and Luigi Preziosi.
    "All biological phenomena emerge from an intricate interconnection of multiple processes occurring at different levels of organization: namely, at the molecular, the cellular and the tissue level, see Figure 1. These natural levels can approximately be connected to a microscopic, mesoscopic, and macroscopic scale, respectively. The microscopic scale refers to those processes that occur at the subcellular level, such as DNA synthesis and duplication, gene dynamics, activation of receptors, transduction of chemical signals, diffusion of ions and transport of proteins. The mesoscopic scale, on the other hand, can refer to cell-level phenomena, such as adhesive interactions between cells or between cells and ECM components, cell duplication and death and cell motion. The macroscopic scale finally corresponds to those processes that are typical of multicellular behavior, such as population dynamics, tissue mechanics and organ growth and development. It is evident that research in biology and medicine needs to work in a multiscale fashion. This brings many challenging questions and a complexity that can not be addressed in the classical way, but can take advantage of the increasing collaboration between natural and exact sciences (for more detailed comments the reader is referred to [90, 262]). On the other hand, the recent literature provides evidence of the increasing attention of the mathematical, statistical, computational and physical communities toward biological and biomedical modeling, consequence of the successful results obtained by a multidisciplinary approach to the Life Sciences problems"-- Provided by publisher.
  • 2009 Springer
    Shireesh P. Apte, Rangaprasad Sarangarajan, editors ; foreword by Gregg L. Semenza.
  • 2009 Springer
    Đurđica Ugarković, editor.
  • 2012 Springer
    Heide Schatten, editor.
    Part I. Centrosomes in Reproduction -- 1. Centriole Duplication and Inheritance in Drosophila melanogaster / Tomer Avidor-Reiss, Jayachandran Gopalakrishnan, Stephanie Blachon and Andrey Polyanovsky -- 2. Sperm Centrioles and Their Dual Role in Flagellogenesis and Cell Cycle of the Zygote: Structure, Function, and Pathology / Hector E. Chemes -- 3. Centrosomal Functions and Dysfunctions in Cat Spermatozoa / Pierre Comizzoli and David E. Wildt -- 4. Nuclear-Centrosome Relationships During Fertilization, Cell Division, Embryo Development, and in Somatic Cell Nuclear Transfer Embryos / Heide Schatten and Qing-Yuan Sun -- 5. Human Centrosomal Dynamics During Gametogenesis, Fertilization, and Embryogenesis and Its Impact on Fertility: Ultrastructural Analysis / A. Henry Sathananthan -- 6. Asymmetric Centrosome Behavior in Stem Cell Divisions / Therese M. Roth, Yukiko M. Yamashita and Jun Cheng -- Part II. Cell and Molecular Biology of Centrosomes -- 7. Functional Associations Between the Golgi Apparatus and the Centrosome in Mammalian Cells / Breanne Karanikolas and Christine Sütterlin -- 8. Many Pathways to Destruction: The Role of the Centrosome in, and Its Control by Regulated Proteolysis / Harold A. Fisk -- 9. Regulation of the Centrosome Cycle by Protein Degradation / Suzanna L. Prosser and Andrew M. Fry -- 10. Molecular Links Between Centrosome Duplication and Other Cell Cycle-Associated Events / Kenji Fukasawa -- 11. Regulation of Centrosomes by Cyclin-Dependent Kinases / Rose Boutros -- Part III. Centrosome Abnormalities in Cancer -- 12. Disruption of Centrosome Duplication Control and Induction of Mitotic Instability by the High-Risk Human Papillomavirus Oncoproteins E6 and E7 / Nina Korzeniewski and Stefan Duensing -- 13. Centrosomes, DNA Damage and Aneuploidy / Chiara Saladino, Emer Bourke and Ciaran G. Morrison -- 14. Centrosome Regulation and Breast Cancer / Zeina Kais and Jeffrey D. Parvin -- 15. The Role of Centrosomes in Multiple Myeloma / Benedict Yan and Wee-Joo Chng -- 16. Centrosomal Amplification and Related Abnormalities Induced by Nucleoside Analogs / Ofelia A. Olivero -- 17. Mechanisms and Consequences of Centrosome Clustering in Cancer Cells / Alwin Krämer, Simon Anderhub and Bettina Maier -- Part IV. Centrosomes in Other Systems -- 18. Re-evaluation of the Neuronal Centrosome as a Generator of Microtubules for Axons and Dendrites / Peter W. Baas and Aditi Falnikar -- 19. Centrosomes and Cell Division in Apicomplexa / Leandro Lemgruber, Marek Cyrklaff and Freddy Frischknecht -- 20. The Centrosome Life Story in Xenopuslaevis / Jacek Z. Kubiak and Claude Prigent -- 21. Role of the MTOC in T Cell Effector Functions / Martin Poenie, Laura Christian, Sarah Tan and Yuri Sykulev -- 22. Thoughts on Progress in the Centrosome Field / Jeffrey L. Salisbury.
  • 2015 ScienceDirect
    [edited by] Renata Basto and Karen Oegema.
    This new volume of Methods in Cell Biology looks at methods for analyzing centrosomes and centrioles. Chapters cover such topics as methods to analyze centrosomes, centriole biogenesis and function in multi-ciliated cells, laser manipulation of centrosomes or CLEM, analysis of centrosomes in human cancers and tissues, proximity interaction techniques to study centrosomes, and genome engineering for creating conditional alleles in human cells.
  • 2012
    Joanna Y. Lee.
    Chromosomal translocations fusing genes that encode centrosome proteins and tyrosine kinases are frequently observed in myeloproliferative neoplasms (MPNs). Although the function of centrosome proteins in these fusions is not well understood, roles that may contribute to proliferative signaling including kinase centrosome localization and constitutive kinase dimerization have long been postulated. To test the relative contributions of localization and dimerization on kinase signaling efficiency, we targeted inducibly dimerizable FGFR1 to several subcellular locations and generated a centrosome localization mutant of the FOP-FGFR1 MPN fusion. Expression in mammalian tissue culture cells followed by substrate phosphorylation assays reveals a statistically significant decrease in kinase signaling effectiveness upon loss of FOP-FGFR1 centrosome localization. Interestingly, wild-type FOP-FGFR1 expression also caused a centrosome localization-dependent ciliogenesis defect. Further studies revealed that the fusion partner, FOP, localizes to centriolar satellites in addition to its previously described centrosomal localization. Centriolar satellites are granules clustered around the centrosome suspected to function in microtubule anchoring, protein trafficking, and ciliogenesis, though their precise function and composition remain unknown. Satellite localization is retained in the FOP-FGFR1 fusion and an increase in phosphotyrosine activity occurs at satellites in FOP-FGFR1 expressing cells. Given the importance of centriolar satellites in ciliogenesis, and the link between ciliogenesis and proliferative signaling, it is possible that increased phosphotyrosine activity at satellites is functionally significant in the progression of MPN. Finally, we assayed for ciliary signaling proteins in chronic myelogenous leukemia (CML) cells. Although FOP-FGFR1 causes a ciliogenesis defect, MPN affects blood cells, which have not been reported to contain cilia. We show here that, although CML cells do not appear to form a primary cilium, they contain masses of stabilized tubulin that co-localize with ciliary proteins. This may be evidence for a form of ciliary signaling in unciliated mammalian cells.
  • 2001 ScienceDirect
    Robert E. Palazzo, Trisha N. Davis.
    Also available: Print – 2001
  • 2013 Springer
    Masakatsu Shibasaki, Masamitsu Iino, Hiroyuki Osada, editors.
    At the forefront of life sciences today is the emerging discipline of chembiomolecular science. This new term describes the integration of the frontier fields of chemical biology, chemistry, and pharmacology. Chembiomolecular science aims to elucidate new biological mechanisms as potential drug targets and enhance the creation of new drug therapies. This book comprises the proceedings of the Uehara Memorial Foundation Symposium 2011, which focused on the most recent advances in chembiomolecular science made by leading experts in the field. The book is divided into three main topics. The first is the chemical approach to understanding complex biological systems on a molecular level using chemical compounds as a probe. The second describes the biological approach used to develop new lead drug compounds. The third focuses on the biological system that serves as the potential drug target, the beginning step in the process of developing new drugs. Replete with the latest research, the book will draw the attention of all scientists interested in the synergies between chemistry and biology to elucidate life on a molecular level and to promote drug discovery. Ultimately, the book helps promote the understanding of biological functions at the molecular level and create new pharmaceuticals that can contribute to improving human health.
  • 2006 Springer
    S. Jaroch, H. Weinmann, editors.
  • v. 1-3, 1970.
    edited by Endre A. Balazs.
    Status: Not Checked OutLane Catalog Record
    v. 1. Collagen, basal laminae elastin --v. 2. Glycosaminoglycans and proteoglycans --v. 3. Structural organization and function of the matrix.
  • 2006 Springer
    Brehon C. Laurent (ed.).
    Also available: Print – 2006
  • 2009 Springer Protocols
    edited by Srikumar P. Chellappan.
    Preparation of chromatin assembly extracts from preblastoderm Drosophila embryos / Edgar Bonte and Peter B. Becker -- Analysis of reconstituted chromatin using a solid-phase approach / Raphael Sandaltzopoulos and Peter B. Becker -- In vivo chromatin decondensation assays : molecular genetic analysis of chromatin unfolding characteristics of selected proteins / Sherry L. Winter, Philip Wong, and Mark G. Alexandrow -- DNA methyltransferase probing of chromatin structure within populations and on single molecules / Carolina Pardo ... [et al.] -- Visualization of the expression of HMGN nucleosomal binding proteins in the developing mouse embryo and in adult mouse tissues / Takashi Furusawa and Michael Bustin -- Drug-induced premature chromosome condensation (PCC) protocols : cytogenetic approaches in mitotic chromosome and interphase chromatin / Eisuke Gotoh -- Analysis of DNA topology in yeast chromatin / Randall H. Morse -- Preparation and analysis of uniquely positioned mononucleosomes / Daria A. Gaykalova ... [et al.] -- Monitoring DNA breaks in optically highlighted chromatin in living cells by laser scanning confocal microscopy / Michael J. Kruhlak, Arkady Celeste, and André Nussenzweig -- Methods to study transcription-coupled repair in chromatin / Hélène Gaillard, Ralf Erik Wellinger, and Andrés Aguilera -- Cytometric analysis of DNA damage : phosphorylation of histone H2AX as a marker of DNA double-strand breaks (DSBs) / Toshiki Tanaka ... [et al.] -- Detection of DNA damage induced by topoisomerase II inhibitors, gamma radiation, and crosslinking agents using the comet assay / Lori A. Hazlehurst -- Analysis of genomic aberrations using comparative genomic hybridization of metaphase chromosomes / Melanie Carless -- In vitro replication assay with mammalian cell extracts / Wasia Rizwani and Srikumar P. Chellappan -- Investigation of genomic methylation status using methylation-specific and bisulfite sequencing polymerase chain reaction / Melanie Carless -- Biochemical analysis of arginine methylation in transcription / Marc Tini, Hina Naeem, and Joseph Torchia -- In vitro histone demethylase assays / Kenji Kokura and Jia Fang -- Histone chaperone as coactivator of chromatin transcription : role of acetylation / Shrikanth S. Gadad ... [et al.] -- Histone deacetylase activity assay / Zhigang Yuan ... [et al.] -- In vitro and in vivo assays for studying histone ubiquitination and deubiquitination / Ling Zhai, Heui-Yun Joo, and Hengbin Wang -- Detection of histone H3 phosphorylation in cultured cells and tissue sections by immunostaining / Jaya Padmanabhan -- Chromatin immunoprecipitation assays : analyzing transcription factor binding and histone modifications in vivo / Smitha Pillai, Piyali Dasgupta, and Srikumar P. Chellappan -- ChIP on Chip assays : genome-wide analysis of transcription factor binding and histone modifications / Smitha Pillai and Srikumar P. Chellappan -- ChIP-on-Chip analysis methods for Affymetrix tiling arrays / Sean J. Yoder and Steven A. Enkemann -- Non-radioactive assay methods for the assessment of telomerase activity and telomere length / Partha P. Banerjee and Shankar Jagadeesh -- Detecting ATM-dependent chromatin modification in DNA damage and heat shock response / Sandeep Misri, Shruti Pandita, and Tej K. Pandita.
  • 2015 Springer Protocols
    edited by Srikumar P. Chellappan.
    Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condensation, and the use of comparative genomic hybridization to assess genomic aberration are included as well. Novel techniques for imaging chromatin using atomic force microscopy and the isolation of specific genomic regions using engineered DNA binding molecules generated by CRISPR are then examined. That section is followed by protocols to analyze DNA and histone modifications, while the third section includes methods to study DNA replication and repair, in the context of chromatin. Last but not least, protocols for studying chromatin and its relation with transcriptional regulation are presented in a fourth section. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromatin Protocols, Third Edition aims to help researchers in facilitating in-depth molecular analysis of various aspects of chromatin structure and function.
  • 2010
    Alyssa Christine Snider.
    Early embryonic cell types such as the zygote, blastomeres of the preimplantation embryo, and embryonic stem (ES) cells have powerful chromatin remodeling activities that facilitate DNA-dependent processes such as transcription and DNA repair. Chd1l encodes a chromatin remodeling factor and is more highly expressed in the inner cell mass (ICM) compared to the whole blastocyst. Chd1l expression is developmentally regulated during a time course of preimplantation development and is present in ES cells. Intriguing expression patterns suggested Chd1l could be a novel regulator of DNA-dependent processes in early developmental cell types. This dissertation describes research undertaken to address the role of Chd1l in chromatin remodeling in the preimplantation embryo and in ES cells. Reducing Chd1l protein to nearly undetectable levels in ES cells reveals that Chd1l is dispensable for ES cell viability, proliferation, and pluripotent morphology. Global gene expression patterns are unaltered by Chd1l knock-down. Chd1l is also dispensable for expression patterns of lineage markers associated with the formation of the primary germ layers in differentiating embryoid bodies. Zygote-stage embryos injected with Chd1l-MO arrest prior to the morula stage. Knock-down by the MO was confirmed at the transcript levels by microfluidic qPCR, and the arrest phenotype was partially rescued upon co-injection of Chd1l mRNA and Chd1l-MO. ES cells are known to have stringent and unique pathways to repair DNA damage to prevent mutation and genomic instability from arising in the organism. Reduction of Chd1l in ES cells confers resistance to induced DNA damage, a finding that contrasts with another study that used a differentiated cell line. Apoptosis induced by over-expression of Chd1l occurs specifically in ES cells and not in their differentiated counterparts. This switch in the effect of Chd1l over-expression during differentiation suggests that the function of Chd1l is very different in ES cells than in differentiated somatic cells. DNA repair through non-homologous end joining (NHEJ) is particularly critical in the zygote to repair the paternal genome. Differentiated somatic cells, but not ES cells also rely heavily on NHEJ. Biochemical evidence supports the involvement of Chd1l in NHEJ. Deficient DNA repair could underlie the Chd1l arrest phenotype. Therefore, Chd1l functions minimally, or not at all, in regulating gene expression and contributes to the DNA damage response in a developmental stage-specific and/or cell type-specific manner.
  • 2010
    Esteban Toro Gomez.
    Bacterial chromosomes are generally ~1000 times longer than the cells in which they reside, and concurrent replication, segregation, and transcription/translation of this crowded mass of DNA poses a challenging organizational problem. At first, it was assumed that DNA is organized randomly inside the cell, much like a bowl of spaghetti. Furthermore, it was argued that segregation of this disordered mass was not carried out by a mitotic machinery as is found in eukaryotes, but rather the by-product of cell growth. Hence, the canonical description of bacterial DNA was that of an unstructured polymer diffusing randomly. Recent advances in cell imaging technology, however, have revealed that the bacterial nucleoid is reliably oriented and highly organized within the cell. Furthermore, the speed of segregation is inconsistent with the old model driven by cell growth. Here, I use a screening approach to identify parS, a 100bp piece of DNA previously found to be anchored to the old cell pole, as the Caulobacter centromere. In direct contrast to other published models, parS segregates ahead of all other sequences, including the origin of replication (Cori), and moving parS away from Cori causes segregation -- but not replication -- to be delayed. The segregation of parS requires the ATPase activity of the ParA protein, and current work is aimed at understanding the molecular details of this process. All of these data point to the presence of an active, mitotic-like machine that drives chromosome segregation in Caulobacter. I also show that the circular Caulobacter chromosome is oriented in relation to the parS anchor site at the old cell pole, so that changing the genomic position of parS causes the entire chromosome to rotate within the cell. Finally, I find that the chromosome is organized into a dense core running down the length of the cell, and I arrive at two possible models for the large-scale structure of this core. Thus, this work forms part of an emerging view of the chromosome as a highly organized structure that is kept in place by simple yet active mechanisms, and whose segregation is insured by a complex dedicated machine.
  • 2009 ScienceDirect
    edited by Stephen M. King and Gregory J. Pazour.
  • 2009 ScienceDirect
    edited by Stephen M. King and Gregory J. Pazour.
  • pt. A-B, 2013 ScienceDirect
    pt. B ScienceDirect
    edited by Wallace F. Marshall.
  • 2009 ScienceDirect
    edited by Stephen M. King, Gregory J. Pazour.
  • 2016 Springer
    Richard J. Cote, Ram H. Datar, editors.
    Preface -- Foreword -- Section I. Technologies for CTC Identification -- 1. Significance of Studying Circulating Tumor Cells -- 2. Affinity-Based Enrichment of Circulating Tumor Cells -- 3. Size-Based and Non-Affinity Based Microfluidic Devices for Circulating Tumor Cell Enrichment and Characterization -- 4. Molecular Assays for the Detection and Molecular Characterization of CTCs -- 5. Cancer Stem Cells and Circulating Tumor Cells Molecular Markers, Isolation Techniques and Clinical Implications -- Section II. Fundamental Studies of CTC -- 6. Circulating Tumor Cells and Tumor Dormancy.- 7. Prevention of Conversion of Tumor Dormancy into Proliferative Metastases -- 8. Genesis of Circulating Tumor Cells through Epithelial-Mesenchymal Transition as a Mechanism for Distant Dissemination -- Section III. CTC Analysis -- 9. CTC Analysis FISH, ISH, Array-CGH and Other Molecular Assays -- 10. Genome-Wide Gene Copy Number Analysis of Circulating Tumor Cells -- 11. Perspectives on the Functional Characterization and In Vitro Maintenance of Circulating Tumor Cells -- 12. Prognostic Implications of CTC in Breast Cancer -- Section IV. Potential Clinical Applications of CTC -- 13. CTC in Advanced Breast Cancer Prognosis, Monitoring and Clinical Utility -- 14. Evolution of Metastatic Disease: The Need for Monitoring and Emerging Therapeutic Opportunities -- 15. CTC for Biomarker and Companion Diagnostic Development -- 16. Perspectives on Clinical Applications of CTCs -- Index.
  • 2011 Springer Protocols
    edited by Kursad Turksen.
    Measuring size-dependent permeability of the tight junction using PEG profiling / Christina M. Van Itallie and James M. Anderson -- Biochemical analysis of claudin-binding compatibility / Christina Ward and Michael Koval -- Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines / Alan S.L. Yu -- Tight junction, intercellular seal as a cell signaling player : protocols for examination of its status / Makoto Osanai -- Interactions between Clostridium perfringens enterotoxin and claudins / Susan L. Robertson and Bruce A. McClane -- Biophysical methods to probe claudin-mediated adhesion at the cellular and molecular level / Sri Ram Krishna Vedula [and others] -- Detection of tight junction barrier function in vivo by biotin / Lei Ding [and others] -- Coculture method to examine interactions between claudin isoforms in tight junction-free HEK293 cells and tight junction-bearing MDCK II cells / Tetsuichiro Inai -- Claudin-4 : functional studies beyond the tight junction / Holly A. Eckelhoefer [and others] -- Methods to analyze subcellular localization and intracellular trafficking of claudin-1 / P. Jaya Kausalya and Walter Hunziker -- Claudin family proteins in Caenorhabditis elegans / Jeffrey S. Simske and Jeff Hardin -- In vivo imaging of tight junctions using claudin-EGFP transgenic medaka / Tatsuo Miyamoto, Mikio Furuse, and Makoto Furutani-Seiki -- Claudins in a primary cultured puffer fish (Tetraodon nigroviridis) gill epithelium / Phuong Bui and Scott P. Kelly -- Manipulating claudin expression in avian embryos / Michelle M. Collins and Aimee K. Ryan -- Identification of claudins by western blot and immunofluorescence in different cell lines and tissues / Lorenza Gonzalez-Mariscal, Erika Garay, and Miguel Quiros -- Expression and function of claudins in hepatocytes / Takashi Kojima and Norimasa Sawada -- Analysis of changes in the expression pattern of claudins using salivary acinar cells in primary culture / Junko Fujita-Yoshigaki -- Development of biological tools to study claudins in the male reproductive tract / Daniel G. Cyr [and others] -- Using molecular tracers to assess the integrity of the intestinal epithelial barrier in vivo / Julian A. Guttman -- Laboratory methods in the study of endometrial claudin-4 / Paulo Serafini [and others] -- Role of claudins in oxidant-induced alveolar epithelial barrier dysfunction / Yu Sun, Richard D. Minshall, and Guochang Hu -- Tracing the endocytosis of claudin-5 in brain endothelial cells / Svetlana M. Stamatovic, Richard F. Keep, and Anuska V. Andjelkovic -- Quantitative in situ analysis of claudin expression at the blood-retinal barrier / Heping Xu and Janet Liversidge -- MMP-mediated disruption of claudin-5 in the blood-brain barrier of rat brain after cerebral ischemia / Yi Yang and Gary A. Rosenberg -- Claudin-5 expression in in vitro models of the blood-brain barrier / Itzik Cooper, Katayun Cohen-Kashi-Malina, and Vivian I. Teichberg -- HIV-1-induced alterations of claudin-5 expression at the blood-brain barrier level / Ibolya E. Andras and Michal Toborek -- Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies / Jane E. Collins [and others] -- Claudin-16/paracellin-1, cloning, expression, and its role in tight junction functions in cancer and endothelial Cells / Tracey A. Martin and Wen G. Jiang -- Dynamics of claudins expression in colitis and colitis-associated cancer in rat / Yoshiaki Arimura, Kanna Nagaishi, and Masayo Hosokawa -- Anti-claudin-4-conjugated highly luminescent nanoparticles as biological labels for pancreatic cancer sensing / Ken-Tye Yong.
  • 2011 Springer
    Sheng Luan, editor.
    Plant cell calcium, past and future / Anthony J. Trewavas -- Calcium signalilng and homeostasis in nuclei / Christian Mazars .. [et al.] --Interactions between calcium and ROP signaling regulate pollen tube tip growth / Augusta Jamin and Zhenbiao Yang -- Calcium, mechanical signaling, and tip growth / Won-Gyu Choi, Sarah J. Swanson, and Simon Gilroy -- Calcium signals in the control of stomatal movements / Alex A.R. Webb and Fiona C. Robertson -- Stimulus perception and membrane excitation in unicellular alga Chlamydomonas / Kenjiro Yoshimura -- Cyclic nucleotide gated channels (CNGCs) and the generation of Ca2+ signals / Wei Ma and Gerald A. Berkowitz -- Annexins / Anuphon Laohavisit and Julia M. Davies -- Structure and function of CDPK : a sensor responder of calcium / Yohsuke Takahashi and Takeshi Ito -- Elucidation of calcium-signaling components and networks / Irene S. Day and A.S.N. Reddy -- Decoding of calcium signal through calmodulin : calmodulin-binding proteins in plants / Liqun Du [and others] / The CBL-CIPK network for decoding calcium signals in plants / Oliver Batistic [and others].
  • 2007 Springer
    Parvin Ganjei-Azar, Mehrdad Nadji.
  • 2016 Springer
    Adebowale J. Adeniran, David Chhieng.
    This text provides a comprehensive review of the most frequently encountered diagnostic pitfalls in thyroid cytopathology. Written in a case study format, the text provides the most current and complete information on both non-neoplastic and neoplastic thyroid conditions. Each chapter focuses on a specific diagnostic challenge and/or pitfalls that help in the differential diagnosis as well as the judicious use of ancillary studies when necessary. A review of the current literature as it relates to each disease entity is also incorporated into respective chapters. Highly illustrated with ample illustration of microscopic features to tackle common and uncommon diagnostic challenge in everyday routine practice. This book provides a concise yet comprehensive summary of the common cytologic features needed to resolve the diagnostic pitfalls. Common Diagnostic Pitfalls in Thyroid Cytopathology serve as a very useful resource for pathologists and physicians dealing with, and interested in the thyroid. .
  • 2015 ClinicalKey
    edited by Marluce Bibbo, David C. Wilbur.
    In the newly updated edition of Comprehensive Cytopathology, a team of international experts provides criteria and techniques in diagnosis, testing, and new insights in cytology. This accessible guide to diagnostic investigation and screening is ideal for daily laboratory use, taking a systematic approach to helping you understand major diagnostic criteria as well as the pitfalls and limitations of cytology. Quickly find the answers you need through a consistent chapter structure.Realize every possibility. Appropriate histopathological correlations and a consideration of the possible differential diagnosis accompany the cytological findings.
  • 2012 ScienceDirect
    edited by Anand R. Asthagiri, Adam P. Arkin.
    Principles of model building : an experimentation-aided approach to development of models for signaling networks / Ambhighainath Ganesan and Andre Levchenko -- Integrated inference and analysis of regulatory networks from multi-level measurements / Christopher S. Poultney, Alex Greenfield, and Richard Bonneau -- Swimming upstream : identifying proteomic signals that drive transcriptional changes using the interactome and multiple "-omics" datasets / Shao-shan Carol Huang and Ernest Fraenkel -- A framework for modeling the relationship between cellular steady-state and stimulus-responsiveness / Paul M. Loriaux and Alexander Hoffmann -- Stochastic modeling of cellular networks / Jacob Stewart-Ornstein and Hana El-Samad -- Quantifying traction stresses in adherent cells / Casey M. Kraning-Rush ... [et al.] -- CellOrganizer : image-derived models of subcellular organization and protein distribution / Robert F. Murphy -- Spatial modeling of cell signaling networks / Ann E. Cowan ... [et al.] -- Stochastic models of cell protrusion arising from spatiotemporal signaling and adhesion dynamics / Erik S. Welf and Jason M. Haugh -- Nonparametric variable selection and modeling for spatial and temporal regulatory networks / Anil Aswani ... [et al.] -- Quantitative models of the mechanisms that control genome-wide patterns of animal transcription factor binding / Tommy Kaplan and Mark D. Biggin -- Computational analysis of live cell images of the Arabidopsis thaliana plant / Alexandre Cunha ... [et al.] -- Multi-scale modeling of tissues using CompuCell3D / Maciej H. Swat ... [et al.] -- Multiscale model of fibrin accumulation on the blood clot surface and platelet dynamics / Zhiliang Xu ... [et al.].
  • 2012 Springer Protocols
    edited by Xuedong Liu, Meredith D. Betterton.
    Predictive models for cellular signaling networks / Dagmar Iber and Georgios Fengos -- Analyzing and constraining signaling networks : parameter estimation for the user / Florian Geier [and others] -- A tutorial on mathematical modeling of biological signaling pathways / Zhike Zi -- Bistability iin one equation or fewer / Graham A. Anderson, Xuedong Liu, and James E. Ferrell, Jr. -- Mathematical investigation of how oncogenic Ras mutants promote Ras signaling / Edward C. Stites and Kodi S. Ravichandran -- Modeling miRNA regulation in cancer signaling systems : miR-34a regulation of the p53/Sirt1 signaling module / Xin Lai, Olaf Wolkenhauer, and Julio Vera -- Design of experiments to investigate dynamic cell signaling models / Samuel Bandara and Tobias Meyer -- Mathematical modeling of biochemical systems with PottersWheel / Thomas Maiwald, Oliver Eberhardt, and Julie Blumberg -- Rule-based modeling of signal transduction : a primer / John A.P. Sekar and James R. Faeder -- Computational modeling of signal transduction networks : a pedagogical exposition / Ashok Prasad -- Modeling spatiotemporal dynamics of bacterial populations / Hao Song and Lingchong You -- Discrete dynamic modeling of signal transduction networks / Assieh Saadatpour and Réka Albert -- Analytic methods for modeling stochastic regulatory networks / Aleksandra M. Walczak, Andrew Mugler, and Chris H. Wiggins.
  • 2013 CRCnetBASE
    edited by Ernesto Oviedo-Orta, Brenda R. Kwak, William Howard Evans.
    Communication in the immune system by connexin channels / E. Oviedo-Orta and W. Howard Evans -- Gap junctions and connexins in the hematopoietic-immune system : structural considerations / T. Krenacs, I. Zsakovics and M. Rosendaal -- Approaches for studying the role(s) of gap junctions in the immune system / A.M. Glass, T.D. Nguyen, S. Taffet -- Gap junctions in antigen presenting cells / P.J. Saez, K.F. Shoji, J.C. Saez -- Connect the immune system : roles of gap junctions in antigen presentation and T cell activation / B. Pang, J. Neefjes -- Gap junctions and connexins in the immune defence against tumors / F. Salazar-Onfray -- Connexins in atherosclerosis / L. Burnier, B.R. Kwak -- Connexins in lung function and inflammation / M. Chanson, M. Koval -- The effect of oxidative stress on connexins in the vasculature / M. Billaud ... [et al.] -- The regulation of gap junctions and cellular coupling within the microvasculature in response to acute inflammation / D. Lidington, K. Tyml -- Impact of microglial activation on astroglial connexin expression and function in brain inflammation / C. Giaume ... [et al.] -- A role for connexins in inflammatory disorders of the epidermis / S. Donnelly, P. Martin -- Translating basic research on Cx43 gap junctions into therapies for reducing scarring and cardiac arrhythmia / J.M. Rhett ... [et al.] -- Connexin-based therapeutic approaches to inflammation in the central nervous system / J. Zhang ... [et al.] -- Enhancing epithelial tissue repair and reducing inflammation by targeting connexins / D.L. Becker ... [et al.].
  • 2015 ScienceDirect
    edited by Catherine Stavropoulos-Giokas, Dominique Charron, Cristina Navarrete.
    Section I: Introduction. Introduction to cord blood stem cells / Dominique Charron, Catherine Stavropoulos-Giokas, Cristina Navarrete. Section II: Cord blood cells biology. Cord blood content / Gesine Kögler [and 3 others] -- Cord blood hematopoiesis : the road to transplantation / Anna Rita Migliaccio, Thalia Papayannopoulu -- Immunobiology of cord blood cells / Sergio Querol, Aurore Saudemont, Antoine Toubert -- Cord and cord blood-derived endothelial cells / Suzanne M. Watt [and 8 others] -- HLA and immunogenetics in cord blood transplantation / Dominique Charron, Emeline Masson, Pascale Loiseau. Section III: Cord blood cells for clinical use. Clinical use of umbilical cord blood cells / Robert Danby, Vanderson Rocha -- Immunodeficiencies and metabolic diseases / Paul J. Orchard, Angela R. Smith -- Cord blood cells and autoimmune diseases / LingYun Sun [and 3 others] -- Umbilical cord as a source of immunomodulatory reagents / Antonio Galleu, Francesco Dazzi -- Cord blood cells for clinical use : expansion and manipulation / Amanda L. Olson, Elizabeth J. Shpall -- Cord blood stem cells for clinical use : diabetes and cord blood / Yong Zhao -- Section IV: Regenerative medicine applications. Emerging uses of cord blood in regenerative medicine : neurological applications / Jessica M. Sun, Joanne Kurtzberg -- Biobanks for induced pluripotent stem cells and reprogrammed tissues / Lee Carpenter. Section V: Cord blood banking : a current state of affairs. Cord blood banking : operational and regulatory aspects / Cristina Navarrete -- Cord blood unit selection for unrelated transplantation / Andromachi Scaradavou -- Quality management systems including accreditation standards / Andreas Papassavas [and 6 others] -- Regulation across the globe / Maria Mitrossili [and 3 others] -- International development and import/export : WMDA / Lydia Foeken, Daniela Orsini. Section VI: Cord blood banking : current and future outlooks. Allogeneic and autologous cord blood banks / Paolo Rebulla -- The future of cord blood banks / Catherine Stavropoulos-Giokas [and 3 others]. Section VII: The viewpoint of society. An introductory note to the cord blood banking issues in a European and international environment / Marietta Giannakou -- Ethical and legal issues in cord blood stem cells and biobanking / Maria Mitrossili, Marcos Sarris, Yannis Nikolados -- Industrial economics of cord blood banks / Gregory Katz -- Public health policies in European Union : an innovation strategy : Horizon 2020 / Sotiris Soulis [and 5 others].
  • 2014 ScienceDirect
    edited by Thomas Müller-Reichert, Paul Verkade.
    This new volume of Methods in Cell Biology looks at methods for analyzing correlative light and electron microscopy (CLEM). With CLEM, people try to combine the advantages of both worlds, i.e. the dynamics information obtained by light microscopy and the ultrastructure as provided by electron microscopy. This volume contains the latest techniques on correlative microscopy showing that combining two imaging modalities provides more than each technique alone. Most importantly it includes the essential protocols, including tips, tricks and images for you to repeat these exciting techniques in your own lab. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.
  • 2012 ScienceDirect
    edited by Thomas Müller-Reichert, Paul Verkade.
  • 2013 Springer
    Ryoichiro Kageyama, Tetsuo Yamamori, editors.
    This book reviews recent progress in cortical development research, focusing on the mechanisms of neural stem cell regulation, neuronal diversity and connectivity formation, and neocortical organization. Development of the cerebral cortex, the center for higher brain functions such as cognition, memory, and decision making, is one of the major targets of current research. The cerebral cortex is divided into many areas, including motor, sensory, and visual cortices, each of which consists of six layers containing a variety of neurons with different activities and connections. As this book explains, such diversity in neuronal types and connections is generated at various levels. First, neural stem cells change their competency over time, giving sequential rise to distinct types of neurons and glial cells: initially deep layer neurons, then superficial layer neurons, and lastly astrocytes. The activities and connections of neurons are further modulated via interactions with other brain regions, such as the thalamocortical circuit, and via input from the environment. This book on cortical development is essential reading for students, postdocs, and neurobiologists.
  • 2002- Wiley
    editorial board, Juan S. Bonifacino ... [et al.].
    ch. 1. Cell Culture -- ch. 2. Preparation and Isolation of Cells -- ch. 3. Subcellular Fractionation and Isolation of Organelles -- ch. 4. Microscopy -- ch. 5. Characterization of Cellular Proteins -- ch. 6. Electrophoresis and Immunoblotting -- ch. 7. Protein Labeling and Immunoprecipitation -- ch. 8. Cell Cycle Analysis -- ch. 9. Cell Adhesion -- ch. 10. Extracellular Matrix -- ch. 11. In Vitro Reconstitution -- ch. 12. Cell Motility -- ch. 13. Organelle Motility -- ch. 14. Signal Transduction: Protein Phosphorylation -- ch. 15. Protein Trafficking -- ch. 16. Antibodies as Cell Biological Tools -- ch. 17. Macromolecular Interactions in Cells -- ch. 18. Cellular Aging and Death -- ch. 19. Whole Organism and Tissue Analysis -- ch. 20. Expression and Introduction of Macromolecules into Cells -- ch. 21. Fluorescent Protein Technology -- ch. 22. Cell Biology of Chromosomes and Nuclei -- ch. 23. Stem Cells -- ch. 24. Lipids -- ch. 25. Nanotechnology -- ch. 26. Viruses -- ch. 27. RNA-Based Methods in Cell Biology -- Appendix 1. Useful Information and Data -- Appendix 2. Laboratory Stock Solutions and Equipment -- Appendix 3. Commonly Used Techniques -- Appendix. Suppliers.
  • 1997- Wiley
    editorial board, J. Paul Robinson, managing editor ... [et al.].
    Consists of a core publication dated Apr. 1997, and updated by supplements 1 (July 1997)-<57 (July 2010)>. Content is peer-reviewed protocols for flow and image cytometry updated quarterly.
  • 2007- Wiley
    editorial board, Thorsten Schlaeger (editor-in-chief), Roger Patient, Evan Snyder, Joseph Wu, Yukiko Yamashita ; past editors, Mick Bhatia, Andrew G. Elefany, Susan J. Fisher.
    Includes methods for isolating, maintaining, and differentiating embryonic and adult stem cells.
  • 2008 Springer
    Nancy Y. Ip, Li-Huei Tsai, editors.
  • 2014 Springer
    Takayuki Yoshimoto, Tomohiro Yoshimoto, editors.
    The Interleukin-1 Family / Charles A. Dinarello and Mihai G. Netea -- IL-6 and Inflammatory Diseases / Daisuke Kamimura, Yasunobu Arima, Toshio Hirano, Hideki Ogura, and Masaaki Murakami -- The Roles of IL-17A and IL-17F in Infection and Inflammatory Disorders / Harumichi Ishigame and Susumu Nakae -- IL-18 / Wen Li, Yoshimasa Tanaka, and Haruki Okamura -- Interleukin-21: a Pleiotropic Mediator of Immunity and Inflammation with Broad Therapeutic Potential / Rosanne Spolski and Warren J. Leonard -- Interleukin-22: A Bridge Between Epithelial Innate Host Defense and Immune Cells / Xiaoting Wang and Wenjun Ouyang -- IL-23 in Health and Disease / Jonathan P. Sherlock, Luis A. Zuniga, and Daniel J. Cua -- IL-36: An Epithelial Cytokine Important in Psoriasis / John E. Sims, Solenne Vigne, Cem Gabay, and Jennifer E. Towne -- Anti-TNF Therapy: 20 Years from Our First Therapeutic Adventure / Jagdeep Nanchahal, Peter Taylor, Richard Williams, and Marc Feldmann -- Interleukin-25: Key Initiator of Type 2 Immune Responses / Hiroshi Nakajima, Tomohiro Tamachi, and Koichi Hirose -- Interleukin-33: Multifunctional Alarmin that Promotes Both Health and Disease / Kazufumi Matsushita and Tomohiro Yoshimoto -- Thymic Stromal Lymphopoietin (TSLP) / Steven F. Ziegler, Florence Roan, Bryan D. Bell, Thomas A. Stoklasek, Masayuki Kitajima, and Hongwei Han -- Interleukin-10: Cytokines in Anti-inflammation and Tolerence / Ashleigh Howes, Philippa Stimpson, Paul Redford, Leona Gabrysova, and Anne O'Garra -- Interleukin-27: Regulation of Immune Responses and Disease Development by a Pleiotropic Cytokine with Pro- and Anti-inflammatory Properties / Izuru Mizoguchi, Kaname Higuchi, Kana Mitobe, Ren Tsunoda, Junichiro Mizuguchi, and Takayuki Yoshimoto -- Interleukin-35: A Novel Mediator of Peripheral Tolerance / Greg M. Delgoffe and Dario A.A. Vignali.
  • 2012 Springer Protocols
    edited by Marc de Ley.
    A tale of two interferon bioassays : how frustration with discrepant results from slightly dissimilar methods can engender discovery / Alfons Billiau -- The use of real-time quantitative PCR for the analysis of cytokine mRNA levels / Maria Forlenza -- Interleukin-27 induces interferon-inducible genes : analysis of gene expression profiles using Affymetrix microarray and DAVID / Tomozumi Imamichi [and others] -- Quantitative analysis of miRNA expression in epithelial cells and tissues / Markus Bitzer [and others] -- Evaluating posttranscriptional regulation of cytokine genes / Bernd Rattenbacher and Paul R. Bohjanen -- Cloning of cytokine 3' untranslated regions and posttranscriptional assessment using cell-based GFP assay / Latifa Al-Haj and Khalid S.A. Khabar -- Integrin-targeted stabilized nanoparticles for an efficient delivery of siRNAs in vitro and in vivo / Charudharshini Srinivasan, Dan Peer, and Motomu Shimaoka -- Hammerhead ribozyme-mediated knockdown of mRNA for fibrotic growth factors : transforming growth factor-beta 1 and connective tissue growth factor / Paulette M. Robinson [and others] -- Control of the interferon response in RNAi experiments / Jana Nejepinska, Matyas Flemr, and Petr Svoboda -- shRNA-induced interferon-stimulated gene analysis / Núria Morral and Scott R. Witting -- Use of RNA interference to investigate cytokine signal transduction in pancreatic beta cells / Fabrice Moore [and others] -- Ligand affinity chromatography, an indispensable method for the purification of soluble cytokine receptors and binding proteins / Daniela Novick and Menachem Rubinstein -- In vitro stimulation and detection of IFN[alpha] production in human plasmacytoid dendritic cells / William C. Adams and Karin Loré -- A sensitive and versatile cytokine bioassay based on type I interferon signaling in 2fTGH cells / Lennart Zabeau, José Van der Heyden, and Jan Tavernier.
  • 2011 CRCnetBASE
    editors, Victor R. Preedy, Ross J. Hunter.
    1. General and cellular aspects -- 2. Life style factor and life events -- 3. Immunology and infections -- 4. Cancer -- 5. Cardiovascular and metabolic disease -- 6. Organs and tissue systems.
  • 2006 Springer
    Dorothy L. Rosenthal, Stephen S. Raab.
  • 2014 ClinicalKey
    [edited by] Edmund S. Cibas, Barbara S. Ducatman.
    Fully updated, the 4th edition of Cytology helps you apply the latest diagnostic techniques for the interpretation of a complete range of cytological specimens. This medical reference book offers access to the newest methods and adjunct tests, all in a brief, highly readable format that makes quick work of reviewing the key information available on this powerful yet minimally invasive method. It's a perfectly practical bench manual for trainees and practicing pathologists. Take advantage of comprehensive yet concise coverage of nearly every type of cytology, including gynecologic, non-gynecologic, and FNA. Understand the clinical implications of your diagnoses and better equip yourself to make effective interpretations with clinical correlations emphasized throughout. Make the most informed reporting decisions through an in-depth analysis of common diagnostic pitfalls. Quickly find the answers you need with succinct, bulleted text, clinical pearls, and summaries of key points. Gain real-life perspective on a complete range of cytologic findings with over 700 full-color illustrations.
  • 2015 Springer
    Ricardo H. Bardales, editor.
  • 2014 Springer
    Ritu Nayar, editor.
    In recent decades, cytopathology has assumed an increasing role in the primary diagnosis of mass lesions owing to its ability to provide rapid, non-invasive, and timely information. With cytopathology at the forefront of diagnosis and prognostication of neoplastic lesions, it is essential that physicians involved in the diagnosis and treatment of precancerous lesions and cancer have a clear understanding of specimen collection techniques, terminology for reporting, and outcomes in various diagnostic categories. This book provides a comprehensive overview of the role of cytology at various body sites. The diagnostic details covered are abbreviated in comparison with those in pathology texts. Instead, a more clinical approach is taken, with the focus on the advantages and limitations of techniques and the key features of entities that are important to clinicians. Pathological clinical correlation is highlighted throughout the book, ensuring that it will be highly relevant for clinicians. In particular, physicians who deal with oncology patients will find it to be a rich source of guidance on how to use and understand cytopathology in the diagnosis and exclusion of malignancy.
    Also available: Print – 2014
  • 2015 Springer
    Yener S. Erozan, Armanda Tatsas.
    This volume fulfills the need for an easy-to-use and authoritative synopsis of intra-abdominal organ cytopathology. Part of the Essentials in Cytopathology book series, the book is heavily illustrated with a full color art program, while the text follows a user-friendly outline format. The book also fits into the lab coat pocket and is ideal for portability and quick reference. Written by authorities in the field, Cytopathology of the Liver, Biliary Tract, Kidney and Adrenal Glands is a concise and valuable resource to pathologists on the subject of intra-abdominal organ cytopathology.
  • 2011 Springer
    Walid E. Khalbuss, Anil V. Parwani.
    & Quot;Soft Tissue Cytopathology" by Anil V. Parwani, MD, PhD and Walid E. Khalbuss, MD, PhD, will fulfill the need for an easy-to-use and authoritative synopsis of soft tissue cytopathology. This volume, part of "The Essentials in Cytopathology book series," fits into the lab coat pocket and is ideal for portability and quick reference. Each volume in the series is heavily illustrated with a full color art program, while the text follows a user-friendly outline format. Anil V. Parwani, MD, PhD, is Associate Professor of Pathology at the University of Pittsburgh Medical Center - S.
  • 2013 Springer
    Gary W. Gill.
    Cytopreparation: Principles & Practice by Gary W. Gill fills a long-standing need for an easy-to-use and authoritative manual on the fundamentals of cytopreparation up-to-and- including microscopy, screening, and data analysis. The text describes in phenomenological terms the most common materials and methods of specimen collection through mounting for gyn, non-gyn, and FNA specimens, as well as the underlying mechanistic bases. The author provides his expertise and information that will empower and enable readers to review and improve their laboratories' cytopreparatory techniques as they apply to the vast majority of specimens. This unique volume provides facts that are not readily available anywhere. Cytopreparation: Principles & Practice is intended for everyone associated with, and involved in, making cytologic preparations that are useful for their intended purpose. It will serve as a valuable reference tool for educators in cytology and histology, cytotechnology and histotechnology students, cytotechnologists, cytopreparatory technicians, cytopathologists, anatomical/clinical pathologists, pathology residents and cytopathology fellows.
  • 2012 Springer
    Maria Kavallaris, editor.
    Part 1. Basics of the Cytoskeleton -- ACTIN / Vera Dugina, Richard Arnoldi, Paul A. Janmey and Christine Chaponnier -- Microtubules / Pavel Dráber and Eduarda Dráberová -- The Kinesin Superfamily / Linda Wordeman -- Basics of the Cytoskeleton: Myosins / Omar A. Quintero, Judy E. Moore and Christopher M. Yengo -- Part 2. Actin and Disease -- The Actin Cytoskeleton and Membrane Organisation in T Lymphocytes / Rhea Cornely, Thomas Grewal and Katharina Gaus -- Thin Filament Diseases of Striated Muscle / Anthony J. Kee and Edna C. Hardeman -- Filamins and Disease / Stephen P. Robertson and Philip B. Daniel -- LIM Kinase and Cancer Metastasis / Alice Schofield and Ora Bernard -- Actin Mutations and Deafness / Matías Morín, Fernando Mayo, Felipe Moreno and Miguel A. Moreno-Pelayo -- Therapeutic Targeting of the Actin Cytoskeleton in Cancer / Teresa Bonello, Jason Coombes, Galina Schevzov, Peter Gunning and Justine Stehn -- Part 3. Microtubules and Disease -- Microtubules as a Target in Cancer Therapy / April L. Risinger and Susan L. Mooberry -- Microtubules, Drug Resistance, and Tumorigenesis / Joshua A. McCarroll and Maria Kavallaris -- Posttranslational Modifications of Tubulin / Suzan K. Chao, Chia-Ping H. Yang and Susan Band Horwitz -- Stathmin and Cancer / Dominic Chi Hiung Ng and Frances Byrne -- The Biology and Pathobiology of Tau Protein / Garth F. Hall -- Tubulin-Related Malformations of Cortical Development / Xavier H. Jaglin, Jamel Chelly and Nadia Bahi-Buisson -- Part 4. Intermediate Filaments and Disease -- Spectrins in Human Diseases / Marie-Christine Lecomte -- Laminopathies / Nadir M. Maraldi and Giovanna Lattanzi -- Desmin and Heart Disease / J. Scott Pattison and Jeffrey Robbins -- Neurodegenerative Diseases and Intermediate Filaments / Rodolphe Perrot and Jean-Pierre Julien.
  • 2013 Springer Protocols
    edited by Rolf Dermeitzel.
    Fluorescence proteins and time-lapse imaging of the cytoskeleton / Carsten Theiss and Karl Meller -- Visualizing in vitro trafficking / John W. Murray -- Interaction of the clycocalyx with the actin cytoskeleton / Mia M. Thi, Eno E. Ebong, David C. Spray, and Sylvia O. Suadicani -- Cytoskeleton dynamics and binding factors / Elena E. Grintsvich and Emil Reisler -- Principles of proteomic approaches to the cytoskeleton / Christinina Loosse, Katrin Marcus, and Bodo Schoenebeck -- Analyzing the cytoskeletal transcriptome: sex differences in rat hypothalamus / Sanda Iacobas, Genevieve Neal-Perry, and Dumitru Andrei Iacobas -- Proteomic approaches for the study of electrical synapses and associated protein-interaction complexes / Cantas Alev, Georg Zoidl, and Rolf Dermietzel -- The cytoskeleton as a pharmacological target in neurodegenerative diseases / Saar Oz and Iliana Gozes -- Neurofilaments: properties, functions, and regulation / Rodolphe Perrot and Joel Eyer -- From an axon into a growth cone after axotomy: a model for cytoskeletal dynamics / Micha E. Spira and Hadas Erez -- Isolation of the postsynaptic density: a specialization of the subsynaptic cytoskeleton / Karl-Heinz Smalla, Patricia Klemmer, and Urusla Wyneken -- Cell-to-cell communication in astroglia and the cytoskeletal impact / Georg Zoidl, Stefan Olk, Audrey Tuchinowitz, and Rolf Dermietzel -- Reactive astrocytes, astrocyte intermediate filament proteins, and their role in the disease pathogenesis / Milos Pekny, Michelle Porritt, Yolanda de Pablo, Marcela Pekna, and Ulrika Wilhelmsson -- Microtubules and associated proteins in oligodendrocytes, the myelin forming cells of the central nervous system / Christiane Richter-Landsberg -- Myelin proteome analysis: methods and implications for the myelin cytoskelton / Olaf Jahn, Stefan Tenzer, Nicole Bartsch, Julia Patzig, and Hauke B. Werner.
  • 2009 Springer Protocols
    edited by Ray H. Gavin.
    High resolution multimode light microscopy of cell migration: long-term imaging and analysis / T. Wollert and G.M. Langford -- Imaging the cytoskeleton in live Xenopus laevis embryos / S. Woolner, A.L. Miller and W.M. Bement -- Live-cell imaging of the cytoskeleton and mitochondrial-cytoskeletal interactions in budding yeast / T.C. Swayne, T.G. Lipkin and L.A. Pon -- Jasplakinolide: an actin-specific reagent that promotes actin polymerization / A. Holzinger -- Analysis of centrosome function and microtubule dynamics by time-lapse microscopy in Xenopus egg extracts / C. Wiese, J.R. Mayers and A.J. Albee -- Quantitative fluorescence microscopy techniques / A. Esposito [and others] -- A FRET-based approach for studying conformational changes of a cytoskeleton-related tumor suppressor molecule / R.F. Hennigan [and others] -- Sample preparation for fluorescence imaging of the cytoskeleton in fixed and living plant roots / J. Dyachok [and others] -- Imaging of the cytoskeleton and mitochondria in fixed budding yeast cells / T.C. Swayne, I.R. Boldogh and L.A. Pon -- Imaging cytoskeleton components by electron microscopy / T. Svitkina -- Purification and localization of intraflagellar transport particles and polypeptides / R.D. Sloboda -- High pressure freezing, electron microscopy, and immuno-electron microscopy of Tetrahymena thermophila basal bodies / J.B. Meehl, T.H. Giddings, Jr. and M. Winey -- Strategies for imaging microtubules in plant cells / A. Holzinger, E. Kawamura and G.O. Wasteneys -- A rapid tracking method for the quantitative analysis of organelle streaming velocity / C.L. Holweg -- Melanosome motility in fish retinal pigment epithelial cells / C. King-Smith -- Analysis of properties of cilia using Tetrahymena thermophila / V. Rajagopalan [and others] -- Application of cell traction force microscopy for cell biology research / J.H. Wang and B. Li -- 2D and 3D quantitative analysis of cell motility and cytoskeletal dynamics / D. Wessels, S. Kuhl and D.R. Soll -- Detergent-extracted models for the study of cilia or flagella / C.B. Lindemann and K.A. Lesich -- The analysis of intermediate filament dynamics using transfections and cell fusions / J.M. Paramio -- Functional analysis of actin-binding proteins in the central nervous system of Drosophila / Q. He -- Proteomic tools for the analysis of cytoskeleton proteins / E. Wywial, V.N. Dongre and S.M. Singh.
  • 2016 Springer Protocols
    edited by Ray H. Gavin.
  • 2011 Springer
    Ralph A. Nixon, Aidong Yuan, editors.
    Central axonal development and pathology in early life / Robin L. Haynes and Hannah C. Kinney -- Microtubules in the nervous system / Nobuyuki Fukushima -- Tau phosphorylation / Jesús Avila and Félix Hernández -- Tau pathology / Nicolas Sergeant and Luc Buée -- Tauopathy and brain aging / Akihiko Takashima -- Microtubule-associated protein 4 / Kiyotaka Tokuraku ... [et al.] -- Structure of neural intermediate filaments / David A.D. Parry -- Alpha-internexin: the fourth subunit of neurofilaments in the mature CNS / Aidong Yuan and Ralph A. Nixon -- Peripherin pathology / Jesse R. McLean and Janice Robertson -- Neurofilament cross-bridge: a structure associated specifically with the neurofilament among the intermediate filament family / Takahiro Gotow -- Neurofilament transport / Andrew J. Grierson and Christopher C.J. Miller -- Knockout models of neurofilament proteins / Rodolphe Perrot and Jean-Pierre Julien -- Neurofilaments and radial growth: deconstruction of a hypothesis through the construction of gene targeted mice / Michael L. Garcia and Devin M. Barry -- Deregulation of cytoskeletal protein phosphorylation and neurodegeneration / Jyotshnabala Kanungo ... [et al.] -- Neurofilaments in aged animals / Shin-ichi Hisanaga, Takahiro Sasaki, and Atsuko Uchida -- Neurofilament changes in multiple sclerosis / Elizabeth Gray and Alastair Wilkins -- Intermediate filament interactions in neurons / Kevin G. Young and Rashmi Kothary -- Tropomyosins in neuronal morphogenesis and development / Nikki Margarita Curthoys, Peter William Gunning, and Thomas Fath -- The driving machinery for growth cone navigation / Takuro Tojima and Hiroyuki Kamiguchi -- Glial fibrillary acidic protein: the intermediate filament protein of astrocytes / Douglas L. Eng and Lawrence F. Eng -- Axonal transport mechanisms in cytoskeleton formation and regulation / Aidong Yuan and Ralph A. Nixon -- Axonal transport and motor neuron disease / Anna-Lena Ström ... [et al.] -- In vivo imaging of axonal transport in aging and Alzheimer's disease / Donna J. Cross and Satoshi Minoshima -- Regulation of cytoskeletal composition in neurons: transcriptional and post-transcriptional control in development, regeneration, and disease / Ben G. Szaro and Michael J. Strong -- Crosstalks between myelinating cells and the axonal cytoskeleton / Rodolphe Perrot and Joël Eyer -- Topographic regulation of neuronal intermediate filament proteins by phosphorylation: in health and disease / Parvathi Rudrabhatla and Harish C. Pant -- Cytoskeleton, axonal transport, and the mechanisms of axonal neuropathy / Hsinlin T. Cheng ... [et al.] -- Effects of insulin on tau and neurofilament / R. Schechter and K.E. Miller -- Ethanol effects on the cytoskeleton of nerve tissue cells / Sergio G. Evrard and Alicia Brusco.
  • 2009 Springer
    Holger Halthoff (ed.).
    The role of TNF in cancer / Harald Wajant -- Many checkpoints on the road to cell death: regulation of Fas-FasL interactions and Fas signaling in peripheral immune responses / Madhu Ramaswamy ... [et al.] -- FasL expression and reverse signaling / M. Lettau ... [et al.] -- Impact of TNF-R1 and CD95 internalization on apoptotic and antiapoptotic signaling / Stefan Schütze and Wulf Schneider-Brachert -- Ubiquitination and TNFR1 signaling / Ken-ichi Fujita and Srinivasa M. Srinivasula -- From biochemical principles of apoptosis induction by TRAIL to application in tumour therapy / Stefanie M. Cordier, Kerstin Papenfuss, and Henning Walczak -- Therapeutic targeting of TWEAK/Fn14 in cancer: exploiting the intrinsic tumor cell killing capacity of the pathway / Jennifer S. Michaelson and Linda C. Burkly -- APRIL in B-cell malignancies and autoimmunity / Fiona C. Kimberly, Jan Paul Medema, and Michael Hahne -- Autophagy in cancer and chemotherapy / Shida Yousefi and Hans-Uwe Simon -- Glucocorticoid-mediated apoptosis resistance of solid tumors / Ingrid Herr, Markus W. Büchler, and Jürgen Mattern -- Targeting death-receptors in radiation therapy / Olivier Niemöller and Claus Belka -- Death ligands designed to kill: development and application of targeted cancer therapeutics based on proapoptotic TNF family ligands / Jeannette Gerspach, Harald Wajant, and Klaus Pfizenmaier.
    Also available: Print – 2009
  • 2005 Springer
    edited by Wafik S. El-Deiry.
  • 2012
    Catherine Amalia Del Vecchio.
    Glioblastoma multiforme (GBM) is the most common and deadly primary brain tumor. Amplification and rearrangements of the epidermal growth factor receptor (EGFR) gene are frequently found in GBM, and the most common variant is EGFRvIII. Here we report that EGFRvIII is a novel cancer stem cell (CSC) or tumor initiating cell marker in GBM. EGFRvIII expression is restricted to sub-regions and sporadic cells within the tumor and EGFRvIII is frequently co-expressed in the putative CSC CD133+ population. EGFRvIII gene and protein expression can be maintained when primary GBM are cultured as tumor derived neurospheres and is lost when spheres are exposed to differentiating conditions. Furthermore, cells that express both EGFRvIII and CD133 display the greatest self-renewal and tumor initiating abilities when compared to the single positive or double negative populations. If amplification and rearrangement are early events in tumorigenesis, this implies that they should be preserved across the tumor despite focal expression. Unexpectedly, we found EGFR amplification and rearrangement throughout the tumor, including regions with no EGFRvIII expression. To study this phenomenon, we characterized three GBM cell lines with endogenous EGFRvIII expression. EGFRvIII defined a hierarchy and epigenetic mechanisms played a role in maintaining heterogeneous EGFRvIII expression, suggesting that drugs which modulate the epigenome might be used successfully in glioblastoma tumors. To assess a broad applicability of our findings to other tumor types, we investigated EGFRvIII expression and amplification in primary breast carcinoma. Our analyses confirmed the presence of EGFRvIII, but in the absence of amplification or rearrangement of the EGFR locus. Interestingly, EGFRvIII was often expressed in a subset of tumor cells and was co-expressed in the putative CSC CD44+/CD24-/low population. EGFRvIII-expressing cells had increased expression of stem cell and epithelial-mesenchymal transition (EMT) associated genes and increased in vitro sphere formation and in vivo tumor formation compared to non-expressing cells. Mechanistically, EGFRvIII mediated its effects through the Wnt/[beta]-catenin pathway. Collectively, this data suggests a novel function for EGFRvIII in tumorigenesis and further supports the use of EGFRvIII-targeted therapy in these tumor types.
  • 2013 Springer
    Sibel Yildirim.
    Dental Evolution -- Tooth development -- Dental Pulp is a Connective Tissue -- Dental Pulp Stem Cells (DPSC) -- Isolation methods of DPSC -- Characterization of DPSC -- Reprogramming of DPSC to induced pluripotent stem cells (iPSC) -- Immunomodulatory effects of DPSC -- Dental Pulp is a Complex Adaptive System.
  • 2012
    Ryan Ward Streble Peacock.
    Studying the effects of gene expression in biological systems has traditionally been undertaken by measuring phenotypic response at a limited number of expression levels. Typically the effects of gene expression are assayed at a high level of overexpression and compared to physiological levels of expression; however, many examples exist in which the level of gene expression greatly affects the resulting cellular phenotypes. Of particular interest for our purposes is the interplay between oncogenes in driving tumorigenesis and cancer progression. For example, oncogene H-Ras is capable of both enhancing cellular proliferation at intermediate levels expression levels (doses) and causing cell cycle arrest and senescence at high expression levels. In order to more thoroughly investigate such behavior we have created a set of tools for measuring dose-dependent effects of gene expression. These tools consist of promoter library to drive wide range gene expression, single-cell proliferation measurements, and data analysis methods to calculate correlation curves between gene expression level and phenotype response. The system is designed for cell-by-cell measuring of both expression level and phenotype response by flow cytometry. This approach allows for full dose experiments to be performed in a single heterogeneous culture with individual cells accessing different expression levels for a given phenotype. Because we are interested in studying the cell biology and genetics of tumor cells, measuring the phenotype of proliferation rate was of particular interest. To aid in further investigation of proliferation rate response to gene overexpression a genetic proliferation reporter was developed. This reporter utilizes the cell cycle-dependent activity of the E2F class of transcription factors, which are active during the G1/S transition. The highly stable fluorescent proteins EGFP and mCherry are expressed from both an E2F-responsive and constitutive promoter, respectively. The ratio of fluorescence levels correlates to proliferation rate in a variety of cell lines, matching predictions made initially by computational model. Finally, given a culture containing expression of a gene or combination of genes by the promoter library and phenotype measurement, the data collected from those cells must be analyzed to determine a correlation curve representing the dose response of the phenotype to level of expression of the genes of interest. In order to calculate this curve from the flow cytometry data several empirical analysis methods were evaluated. We determined the locally weighted scatter plot smoothing (LOWESS) curve fitting method to be best for our purposes of fitting the dose-response curves. As the LOWESS fits of flow cytometry data represented a technical hurdle to performing the dose-response experiments a custom software package was created to allow for easy implementation of the constitutive promoter library for studying dose-dependent behavior. The result is a set of biological and computational tools that allow for high throughput evaluation of gene dose effects. Further effort has been undertaken to move the system to evaluate multiple genes simultaneously, with the ultimate goal of being able to measure genetic cooperation.
  • 2011
    Michael Anthony Thompson.
    Fluorescence microscopy is one of the most widely used tools in cell biology due its intrinsically high detection sensitivity coupled with the ability to genetically label proteins and other cellular structures with fluorescent tags. However, the resolution of fluorescence microscopy has historically been limited to about 200 nm laterally and 800 nm axially because of the diffraction limit of visible light. In the past five years, imaging below the diffraction limit ("super-resolution imaging") by localizing single fluorophores, one at a time (1-3), has opened a wide a variety of new biological systems for study. This Dissertation is a collection of both techniques for two and three dimensional super-resolution imaging as well as applications in bacterial and yeast imaging. References 1. Betzig E, et al (2006) Imaging intracellular fluorescent proteins at nanometer resolution. Science 313: 1642-1645. 2. Hess ST, Girirajan TPK & Mason MD (2006) Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys J 91: 4258-4272. 3. Rust MJ, Bates M & Zhuang X (2006) Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3: 793-795.
  • 2014 Springer Protocols
    edited by David J. Carroll, Stephen A. Stricker.
    Culturing larvae of marine invertebrates / Richard R. Strathmann -- Obtaining gametes and embryos of ascidians / Charles C. Lambert -- Artificial seawater culture of the gastropod Crepidula fornicate for studies of larval settlement and metamorphosis / Anthony Pires -- Trichoplax adhaerens, an enigmatic basal metazoan with potential / Andreas Heyland, Roger Croll, Sophie Goodall, Jeff Kranyak, and Russell Wyeth -- Rearing gymnolaemate bryozoan larvae for cellular and molecular analyses / Michael H. Temkin -- An invertebrate embryologist's guide to routine processing of confocal images / George von Dassow -- TEM analyses of chaetognath reproductive organs / George L. Shinn -- SEM analysis of marine invertebrate gametes / John Buckland-Nicks -- Imaging neural development in embryonic and larval sea urchins / Oliver Krupke, Shunsuke Yaguchi, Junko Yaguchi, and Robert D. Burke -- Preparation and use of sea urchin egg homogenates / Anthony J. Morgan and Antony Galione -- Microinjection and 4D fluorescence imaging in the eggs and embryos of the ascidian Phallusia mammillata / Alex McDougall, Karen Wing-man Lee, and Remi Dumollard -- Isolating specific embryonic cells of the sea urchin by FACS / Celina Juliano, S. Zachary Swartz, and Gary Wessel -- Biotinylation of oocyte cell surface proteins of the starfish Patiria miniata / Samantha Cihal and David J. Carroll -- Perturbations to the hedgehog pathway in sea urchin embryos / Jacob F. Warner and David R. McClay -- Regulation of DNA synthesis at the first cell cycle in the sea urchin in vivo / Jolanta Kisielewska and Michael Whitaker -- Immunoblotting analyses of changes in protein phosphorylations during oocyte maturation in marine nemertean worms / Jose R. Escalona and Stephen A. Stricker -- Multicolor labeling in developmental gene regulatory network analysis / Aditya J. Sethi, Robert C. Angerer, and Lynne M. Angerer -- A 96-well plate format for detection of marine zooplankton with the sandwich hybridization assay / Julio B.J. Harvey -- Isolation and assessment of signaling proteins from synchronized cultures during egg activation and through the egg-to-embryo transition in sea urchins / Michelle M. Roux-Osovitz and Kathy R. Foltz -- Tools for sea urchin genomic analysis / R. Andrew Cameron -- Antibody inhibition of protein activity in starfish oocytes / Eiichi Okumura, Masatoshi Hara, and Takeo Kishimoto -- Immunophotoaffinity labeling of the binding proteins for 1-methyladenine, an oocyte maturation-inducing hormone of starfish / Tetsuo Kida, Shinjiro Matsuda, Atsushi Kuyama, and Tetsuo Toraya.
  • 2015 Springer
    Paolo A. Ascierto, David F. Stroncek, Ena Wang, editors.
    1. New insight of peptide vaccination in cancer immunotherapy -- 2. The determinants of T cell function for effective anticancer vaccine -- 3. T cell fate in the tumor microenvironment -- 4. T cell receptor avidity and affinity and tumor specific TCR engineer -- 5. Host genetic variation and somatic alteration associated with favorable or compromised T cell function -- 6. Production of Clinical T Cell Therapies -- 7. Clinical success of adoptive cell transfer therapy using tumor infiltrating lymphocytes.-8. Harnessing stem cell-like memory T cells for adoptive cell transfer therapy of cancer -- 9. T cell blockade- anti-CTLA4 immunotherapy against cancer and Abscopal effect in combination therapy -- 10. T cell modulation- anti-OD-1 antibodies for the treatment of cancer -- 11. T cell based therapies in combination with other procedures -- 12. Chimeric antigen receptor T cells CD19 CAR -- 13. Hematopoietic Stem Cell transplantation as immune therapy of malignancies.
  • 2015 Springer
    Walid E. Khalbuss, Qing Kay Li, editors.
    Diagnostic Cytopathology Board Review and Self-Assessment provides a comprehensive systems-based review of non-gynecological cytology including cytomorphology, pitfalls and ancillary studies, presented in a high-yield format with board-type multiple choice questions and detailed answers. It provides an excellent review, resource, and self-assessment for pathologists, cytopathologists, and cytotechnologists, as well as trainees (pathology residents, cytopathology fellows, and cytotechology students) who are preparing for board examinations or in-service examinations, in addition to those who are looking to fine-tune their cytology diagnostic skills. The volume is organized in a systems-based format including chapters covering lung, lymph nodes, pancreas, liver, gastrointestinal tract, thyroid, salivary gland, central nervous system, soft tissue and bone, kidney, adrenal, retroperitoneal, and exfoliative cytology of urine, serous fluids, and CSF. This book is enriched with high yield review, board exam-type questions, and high quality full-color images to maximize studying and review of cytopathology.
  • 2013 ClinicalKey
    edited by Gabrijela Kocjan, Winifred Gray, Tanya Levine, Ika Kardum-Skelin, Philippe Vielh.
    Introduction -- Female genital tract -- Respiratory -- Serous effusions -- Urine cytology -- Thyroid gland -- Haemopoietic -- Breast -- Salivary gland -- Liver, biliary tree and pancreas -- Childhood tumours -- Miscellaneous -- Techniques -- Self-assessment questions.
  • 2010 ClinicalKey
    edited by Winifred Gray, Gabrijela Kocjan.
    Cytopathology: the history, the present and the future direction -- Respiratory system -- Respiratory tract -- Serous cavities -- Serous effusions -- Breast -- Alimentary system -- Salivary glands -- Oral cavity -- Oesophagus and gastrointestinal tract -- Hepatobiliary system and pancreas -- Liver -- Gall bladder and extrahepatic bile ducts -- Pancreas -- Kidney and urinary tract -- Kidney and retroperitoneal tissues -- Urine cytology -- Lymphoreticular system -- Lymph nodes -- Other lymphoreticular organs -- Transplantation and immunosuppression -- Organ transplantation -- Immunosuppression -- Endocrine system -- Thyroid gland -- Other endocrine organs -- Male genital tract -- Prostate gland -- Testis and scrotum: cytology of testicular and scrotal masses and male infertility -- Female genital tract -- Vulva, vagina and cervix: normal cytology, hormonal and inflammatory conditions -- Cervical screening programmes -- Cervical intraepithelial neoplasia and squamous cell carcinoma of the cervix -- Glandular neoplasms of the cervix -- Other tumours and lesions of cervix, vulva and vagina -- Cytology of the body of the uterus -- Ovaries, fallopian tubes and associated lesions -- Skin, soft tissues and musculoskeletal system -- Skin -- Soft tissue and musculoskeletal system -- Synovial fluid -- Central nervous system -- Brain and cerebrospinal fluid -- Eyelids, orbit and eye -- Childhood tumours -- New techniques.
  • 2013 ScienceDirect
    edited by Greenfield Sluder and David E. Wolf.
  • 2008 Springer
    edited by Martino Bolognesi, Guido di Prisco, Cinzia Verde.
    Following their own brilliant careers in haeme protein research, the outstanding scientists Jonathan and Beatrice Wittenberg continue to provide inspiration to the research community in the study of oxygen-binding proteins. Their research has provided the intellectual stimulus to bring together scientists from all over the world with the common goal of developing fascinating new ideas and performing innovative experiments. This book is dedicated to Jonathana (TM)s and Beaa (TM)s lifetime careers. It further illuminates the facts and ideas which dot the paths they traced in Biochemistry and Physiology, elaborating on how these landmark achievements were made and how the haeme proteins community still refers to them. With the field of haeme protein science such a flourishing area, the contributors to this book predict Jonathan and Bea, having played such a seminal role, will continue to be key figures for quite some time to come.
  • 2012
    Kristen Jones Holmes.
    Whi3 is an RNA binding protein that regulates cell cycle entry by binding to mRNA of the G1 cyclin CLN3 and to the cyclin-dependent kinase Cdc28. Cln3 binds to Cdc28 to promote passage through Start. whi3[delta] cells have a small size phenotype, and Whi3 may regulate cell size by sequestering CLN3 mRNA and Cdc28 protein in the cytoplasm to delay G1 entry (Wang et al., 2004). We observe that Whi3 reversibly localizes from the cytosol to cytoplasmic puncta in response to glucose deprivation and heat shock. This phenotype is shared by components of stress granules (SGs), which are large RNP complexes that form in yeast in response to various stress conditions; SGs sort and process mRNAs during the stress response (Buchan et al., 2008). Because Whi3 shares several characteristics with known SG components, including an RNA binding domain and stress-dependent relocalization, we hypothesize that Whi3 is a novel component of SGs. We show that Whi3 colocalizes with SG markers in response to glucose deprivation and heat shock. Preventing SG formation inhibits Whi3 foci formation. Like SG components, Whi3 is enriched in insoluble cell fractions during stress. Whi3 is not required for SG formation, but may be targeted to SGs to carry out a previously unknown function. We conclude that Whi3 is a novel component of yeast stress granules. This observation suggests an alternative model for Whi3 function: that Whi3 might regulate mRNA translation during stress by transporting mRNA to stress granules. We demonstrate that Whi3 regulates many of its interacting RNAs, and that this regulation may promote stress-dependent survival.
  • 2005 Springer
    E.J.H.J Wiertz and M. Kikkert, eds.
    The secretory capacity of a cell depends on the efficiency of endoplasmic reticulum-associated degradation / M. Molinari and R. Sitia -- Recognition and delivery of ERAD substrates to the proteasome and alternative paths for cell survival / A.A. McCracken and J.L. Brodsky -- CPY* and the power of yeast genetics in the elucidation of quality control and associated protein degradation of the endoplasmic reticulum / D.H. Wolf and A. Schäfer -- The role of the ubiquination machinery in dislocation and degradation of endoplasmic reticulum proteins / M. Kikkert, G. Hassink, and E. Wiertz -- The role of p97/Cdc48p in endoplasmic reticulum-associated degradation : from the immune system to yeast / S. Bar-Nun -- The ins and outs of intracellular peptides and antigen presentation by MHC Class I molecules / T. Groothuis and J. Neefjes -- Entry of protein toxins into mammalian cells by crossing the endoplasmic reticulum membrane : co-opting basic mechanisms of endoplasmic reticulum-associated degradation / J.M. Lord , L.M. Roberts, and W.I. Lencer.
    Also available: Print – 2005
  • 2007 Springer
    A.B. Tonchev, T. Yamashima, G.N. Chaldakov.
  • 2010 Springer
    M. Celeste Simon, editor.
    The HIF-2[alpha]-driven pseudo-hypoxic phenotype in tumor aggressiveness, differentiation, and vascularization / A. Pietras, A.S. Johnsson and S. Pahlman -- Hypoxia and hypoxia inducible factors in cancer stem cell maintenance / Z. Li and J.N. Rich -- Role of carcinoma-associated fibroblasts and hypoxia in tumor progression / A.J. Giaccia and E. Schipani -- The role of hypoxia regulated microRNAs in cancer / R. McCormick ... [et al.] -- Oxygen sensing: A common crossroad in cancer and neurodegeneration / A. Quaegebeur and P. Carmeliet -- Hypoxia-inducible factors as essential regulators of inflammation / H.Z. Imtiyaz and M.C. Simon -- Hypoxia and metastasis in breast cancer / H. Rundqvist and R.S. Johnson.
    Also available: Print – 2010
  • 2008 Springer
    M. Shakibaei, C. Csaki, and A. Mobasheri.
  • 2013 Springer Protocols
    edited by Svetlana Makovets.
    DNA electrophoresis: historical and theoretical perspectives / Gareth A. Roberts and David T.F. Dryden -- Basic DNA electrophoresis in molecular cloning: a comprehensive guide for beginners / Svetlana Makovets -- Analysis of branched DNA replication and recombination intermediates from prokaryotic cells by two-dimensional (2D) native-native agarose gel electrophoresis / Nicholas P. Robinson -- Analysis of DNA structures from eukaryotic cells by two-dimensional native-native DNA agarose gel electrophoresis / Andreas S. Ivessa -- Directionality of replication fork movement determined by two-dimensional native-native DNA agarose gel electrophoresis / Andreas S. Ivessa -- Native/denaturing two-dimensional DNA electrophoresis and its application to the analysis of recombination intermediates / Jessica P. Lao, Shangming Tang, and Neil Hunter -- Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis / Jorge B. Schvartzman [and three others] -- A neutral glyoxal gel electrophoresis method for the detection and semi-quantitation of DNA single-strand breaks / Brian Pachkowski and Jun Nakamura -- Denaturing gradient gel electrophoresis (DGGE) / Fiona Strathdee and Andrew Free -- Polyacrylamide temperature gradient gel electrophoresis / Viktor Viglasky -- Separation of DNA oligonucleotides using denaturing urea PAGE / Fiona Flett and Heidrun Interthal -- Pulsed-field gel electrophoresis of bacterial chromosomes / Julia S.P. Mawer and David R.F. Leach -- Resolution of budding yeast chromosomes using pulsed-field gel electrophoresis / Aziz El Hage and Jonathan Houseley -- Analysis of DNA damage via single-cell electrophoresis / Diana Anderson and Julian Laubenthal -- Fluorescence in situ hybridization on electrophoresed cells to detect sequence specific DNA damage / Julian Laubenthal and Diana Anderson -- Analysis of DNA-protein interactions using PAGE: band-shift assays / Lynn Powell -- Assaying cooperativity of protein -- DNA interactions using agarose gel electrophoresis / Tanya L. Williams and Daniel L. Levy -- DNA bending by proteins: utilizing plasmid pBendAT as a tool / Fenfei Leng -- Using PCR coupled to PAGE for detection and semiquantitative evaluation of telomerase activity / Laura Gardano.
  • 2013 Springer
    Lesley A. Mathews, Stephanie M. Cabarcas, Elaine M. Hurt, editors.
    The existence of 'cancer stem cells' (CSCs) has been a topic of heated debate for the last few years within the field of cancer biology. Their continuous characterization in a variety of solid tumors has lead to an abundance of evidence supporting their existence. CSCs are believed to be responsible for resistance against conventional treatment regimes of chemotherapy and radiation, ultimately, leading to metastasis and patient demise. To help aid clinicians, pharmaceutical companies and academic labs investigating how to better kill these highly aggressive cells we have summarized the DNA repair mechanism(s) and their role in the maintenance and regulation of both normal and cancer stem cells. Our book represents a comprehensive investigation into the highly effective DNA repair mechanisms of CSCs and what we need to understand in order to develop more advanced therapies to eradicate them from patients. Currently, there are no other published works entirely on DNA repair and Cancer Stem Cells. In addition, our book provides a comprehensive overview of CSC isolation and characterization from a variety of solid tumor types.
  • 2015 Springer Protocols
    edited by Diana P. Bratu, Gerard P. McNeil.
    Drosophila melanogaster oogenesis : an overview / John M. McLaughlin and Diana P. Bratu -- Basic techniques in Drosophila ovary preparation / Letitia Thompson, Kristen Randolph, and Amanda Norvell -- Mosaic analysis in the Drosophila melanogaster ovary / Thomas Rubin and Jean-René Huynh -- Genetic mosaic analysis of stem cell lineages in the Drosophila ovary / Kaitlin M. Laws and Daniela Drummond-Barbosa -- Culturing Drosophila egg chambers and investigating developmental processes through live imaging / Lathiena Manning and Michelle Starz-Gaiano -- Border cell migration : a model system for live imaging and genetic analysis of collective cell movement / Mohit Prasad [and four others] -- Visualizing microtubule networks during Drosophila oogenesis using fixed and live Imaging / Kevin Legent, Nicolas Tissot, and Antoine Guichet -- Visualization of actin cytoskeletal dynamics in fixed and live Drosophila egg chambers / Christopher M. Groen and Tina L. Tootle -- Single-molecule RNA in situ hybridization (smFISH) and immunofluorescence (IF) in the Drosophila egg chamber / Livia V. Bayer [and three others] -- Fluorescent in situ hybridization of nuclear bodies in Drosophila melanogaster ovaries / Zehra F. Nizami, Ji-Long Liu, and Joseph G. Gall -- Ultrastructural analysis of Drosophila ovaries by electron microscopy / Thomas R. Hurd [and seven others] -- Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing piRNA biogenesis bodies in Drosophila ovaries / Shinsuke Shibata [and six others] -- Visualizing cytoophidia expression in Drosophila follicle cells via immunohistochemistry / Ömür Y. Tastan and Ji-Long Liu -- Detection of cell death and phagocytosis in the Drosophila ovary / Tracy L. Meehan [and three others] -- Analysis of cell cycle switches in Drosophila oogenesis / Dongyu Jia , Yi-Chun Huang , and Wu-Min Deng -- Global run-on sequencing (GRO-seq) library preparation from Drosophila ovaries / Nikolay V. Rozhkov -- Bioinformatics analysis to identify RNA-protein interactions in oogenesis / Ravinder Singh.
  • 2009 Springer
    Kiyotaka Toshimori.
    In humans, male infertility is caused by a number of factors such as the external environment, nutrient changes, genotoxins, and mutagens. This book discusses the events that occur in the normal sperm head and govern the structure-function relationship from the time of spermatogenesis to that of fertilization or egg activation.
  • 2012 ScienceDirect
    Stephen M. King.
    Research on dyneins has a direct impact on human diseases, such as viruses and cancer. With an accompanying website showing over one hundred streaming videos of cell dynamic behavior for best comprehension of material, Dynein: Structure, Biology and Disease is the only reference covering the structure, biology and application of dynein research to human disease. From bench to bedside, Dynein: Structure, Biology and Disease offers research on fundamental cellular processes to researchers and clinicians across developmental biology, cell biology, molecular biology, biophysics, biomedicine, genetics and medicine. . Broad-based up-to-date resource for the dynein class of molecular motors . Chapters written by world experts in their topics . Numerous well-illustrated figures and tables included to complement the text, imparting comprehensive information on dynein composition, interactions, and other fundamental features.
  • 2013 Springer
    D. James Morré, Dorothy M. Morré.
    "ENOX Proteins" documents a unique family of cell surface proteins which are the exclusive discovery (subsequently confirmed by others) of the authors, their students and their colleagues. An ENOX-based mechanism is provided for how cells increase in size that is both unique and applicable not only to cancer and cancer therapy but also to production agriculture and biomass increase. The role of ENOX proteins in biological time-keeping is described along with evidence for oscillations in the ratios of electron spin pairs defining ortho and para water states that form highly coordinated populations of coherent water of interest to physicists as the underlying mechanism. The age-related NADH oxidases that appear around age 30 and increase steadily thereafter with potentially causal involvements in atherogenesis and skin aging have been identified as five members of the TM-9 protein superfamily of all with different chromosomal locations. The arNOX proteins initially are membrane anchored but functionally-active N-terminal fragments that are shed into body fluids. Except for critical functional motifs, all of the ENOX protein family members have distinct protein sequences and chromosomal origins. A fourth member of the ENOX protein family occurs in plants as the primary target for both natural and synthetic growth hormone (auxin)-stimulated rapid cell enlargement. Despite masquerading as intractable and difficult to clone and characterize, ENOX proteins offer remarkable opportunities for research, commercial development and outside confirmation of new paradigms to help explain complex biological processes.
  • 2012 Springer
    Wen G. Jiang, editor.
    Electric Cell-Substrate Impedance Sensing Concept to Commercialization / Ivar Giaever and Charles R. Keese -- Protein Kinase C Isoforms in the Formation of Focal Adhesion Complexes: Investigated by Cell Impedance / Havovi Chichger, Katie L. Grinnell and Elizabeth O. Harrington -- ECIS as a Tool in the Study of Metastasis Suppressor Genes: Epithelial Protein Lost In Neoplasm (EPLIN) / Andrew J. Sanders, Vladimir M. Saravolac, Malcolm D. Mason and Wen G. Jiang -- Electrical Cell-Substrate Impedance Sensing for Measuring Cellular Transformation, Migration, Invasion, and Anticancer Compound Screening / Bryan Plunger, Chang Kyoung Choi and Tim E. Sparer -- Epithelial-Mesenchymal Transition and the Use of ECIS / Jane Lane and Wen G. Jiang -- Cell Growth and Cell Death Studied by Electric Cell-Substrate Impedance Sensing / Judith Anthea Stolwijk, Stefanie Michaelis and Joachim Wegener -- Tight Junctions in Cancer Metastasis and Their Investigation Using ECIS (Electric Cell-Substrate Impedance Sensing) / Tracey A. Martin and Wen G. Jiang -- Epithelial Wound Healing and the Effects of Cytokines Investigated by ECIS: Electric Cell-Substrate Impedance Sensing in Epithelial Research / Katalin Szaszi, Matthew Vandermeer and Yasaman Amoozadeh -- Tumour-Endothelial and Tumour-Mesothelial Interactions Investigated by Impedance Sensing Based Cell Analyses / Wen G. Jiang, Lin Ye, Haiying Ren, Ann Kift-Morgan and Nicholas Topley, et al. -- Application of Electric Cell-Substrate Impedance Sensing in Evaluation of Traditional Medicine on the Cellular Functions of Gastric and Colorectal Cancer Cells / Lin Ye, Ke Ji, Jiafu Ji, Rachel Hargest and Wen G. Jiang -- Electric Cell-Substrate Impedance Sensing as a Screening Tool for Wound Healing Agents / Cheuk Lun Liu, Jacqueline Chor Wing Tam, Andrew J. Sanders, David G. Jiang and Chun Hay Ko, et al. -- ECIS, Cellular Adhesion and Migration in Keratinocytes / David C. Bosanquet, Keith G. Harding and Wen G. Jiang -- Current and Future Applications of ECIS Models to Study Bone Metastasis / Lin Ye, Sivan M. Bokobza, Howard G. Kynaston and Wen G. Jiang.
  • 2010 ScienceDirect
    edited by Thomas Müller-Reichert.
    Chapter 1. Electron Microscopy of Viruses -- Chapter 2. Bacterial TEM : New Insights from Cryo-Microscopy -- Chapter 3. Analysis of the Ultrastructure of Archaea by Electron Microscopy -- Chapter 4. Chlamydomonas : Cryopreparation Methods for the 3-D Analysis of Cellular Organelles -- Chapter 5. Ultrastructure of the Asexual Blood Stages of Plasmodium falciparum -- Chapter 6. Electron Tomography and Immuno-labeling of Tetrahymena thermophila Basal Bodies -- Chapter 7. Electron Microscopy of Paramecium (Ciliata).
  • 2013 Springer Protocols
    edited by Nicholas Zavazava.
    Immune privilege of stem cells / Naoki Ichiryu and Paul J. Fairchild -- Immunogenicity of in vitro maintained and matured populations : potential barriers to engraftment of human pluripotent stem cell derivatives / Chad Tang, Irving L. Weissman, and Micha Drukker -- Hematopoietic and nature killer cell development from human pluripotent stem cells / Zhenya Ni, David A. Knorr, and Dan S. Kaufman -- Evaluation of immunogenicity of rat ES-cell derived endothelial cells / Martina Seifert and Juliane Ladhoff -- Interaction of ES cell derived neural progenitor cells with natural killer cells and cytotoxic T cells / Casimir de Rham and Jean Villard -- Strategies to generate induced pluripotent stem cells / Michael Hayes and Nicholas Zavazava -- Differentiation and lineage commitment of murine embryonic stem cells into insulin producing cells / Sudhanshu P. Raikwar and Nicholas Zavazava -- Mouse ES cell-derived hematopoietic progenitor cells / Eun-Mi Kim, Gohar Manzar, and Nicholas Zavazava -- Directed differentiation of embryonic stem cells to the T-lymphocyte lineage / Haydn C.-Y. Liang, Roxanne Holmes, and Juan Carlos Zúñiga-Pflücker -- Development of hematopoietic stem and progenitor cells from mouse embryonic stem cells, in vitro, supported by ectopic human HOXB4 expression / Sandra Pilat, Sebastian Carotta, and Hannes Klump -- Histone modification profiling in normal and transformed human embryonic stem cells using micro chromatin immunoprecipitation, scalable to genome-wide microarray analyses / Angelique Schnerch, Shravanti Rampalii, and Mickie Bhatia -- Combined total proteomic and phosphoproteomic analysis of human pluripotent stem cells / Junjie Hou ... [et al.] -- Transcriptional regulatory mechanisms that govern embryonic stem cell fate / Satyabrata Das and Dana Levasseur.
  • v. 1-2, 2006 Springer Protocols
    v. 2, 2006 Springer Protocols
    edited by Kursad Turksen.
    v. 1. Isolation and characterization -- v. 2. Differentiation models.
  • 2006 ScienceDirect
    edited by Irina Klimanskaya, Robert Lanza.
    Derivation and maintenance of embryonic stem cells -- Differentiation of embryonic stem cells.
    Also available: Print – 2006
  • 2007 Springer
    edited by John R. Masters, Bernhard O. Palsson and James A. Thomson.
    Defined culture media for human embryonic stem cells / T. Ludwig and J. Thomson -- Generation of disease-specific human embryonic stem cell lines / S. Minger -- Characterization and differentiation of human embryonic stem cells / A. Laslett ... [et al.] -- Genetic modification of human embryonic stem cells / T. Zwaka -- Hematopoietic differentiation / C. Cerdan ... [et al.] -- Neural differentiation / Z.-J. Zhang ... [et al.] -- Germ cell differentiation / V. Angeles and R. Pera -- Mesodermal differentiation / N. Sharon and N. Benvenisty -- Three-dimensional culture of human embryonic stem cells / S. Gerecht ... [et al.] -- Extraembryonic cell differentiation / L. Armstrong and M. Lako -- Pancreatic cell differentiation / B. Fishman ... [et al.] -- Cardiomyocyte differentiation / D. Singla ... [et al.] -- Human embryonal carcinoma (EC) cells: Complementary tools for embryonic stem cell research / P. Tonge and P. Andrews -- Quality control of human stem cell lines / G. Stacey.
  • 2013 Springer
    Huang-Ge Zhang, editor.
  • 2013 Springer
    Michael K. Danquah, Ram I. Mahato, editors.
  • 2013 Springer
    edited by Robert H. Kretsinger, Vladimir N. Uversky, Eugene A. Permyakov.
    In biochemistry, a metalloprotein is a generic term for a protein that contains a metal cofactor. The metal may be an isolated ion or may be coordinated with a nonprotein organic compound, such as the porphyrin found in hemoproteins. In some cases, the metal is co-coordinated with a side chain of the protein and an inorganic nonmetallic ion. This kind of protein-metal-nonmetal structure is seen in iron-sulfur clusters Metalloproteins deals with all aspects related to the intracellular and extracellular metal-binding proteins, including their structures, properties and functions. The biological roles of metal cations and metal-binding proteins are endless. They are involved in all crucial cellular activities. Many pathological conditions are related to the problematic metal metabolism. Research in metalloprotein-related topics is therefore rapidly growing, and different aspects of metal-binding proteins progressively enter curricula at Universities and even at the High School level on occasion. However, no key resource providing basic, but comprehensible knowledge on this rapidly expanding field exists. The Encyclopedia of Metalloproteins aims to bridge this gap, and will attempt to cover various aspects of metalloprotein/metalloproteomics and will deal with the different issues related to the intracellular and extracellular metal-binding proteins, including their structures, properties and functions. The goal is to cover exhaustively all catalytically and biologically crucial metal ions and to find at least one interacting protein for other metal ions. The Encyclopedia of Metalloproteins will provide a key resource for advanced undergraduate and graduate students, researchers, instructors, and professors interested in protein science, biochemistry, cell biology, and genetics.
  • 2012 Springer
    Sangdun Choi, editor.
  • 2009 Springer
    John A. Maksem, Stanley J. Robboy, John W. Bishop, Isabelle Meiers.
  • 2012 Springer
    edited by Patrizia Agostinis, Afshin Samali.
    The Endoplasmic Reticulum (ER) is an organelle with extraordinary signaling and homeostatic functions. It is the organelle responsible for protein folding, maturation, quality control and trafficking of proteins destined for the plasma membrane or for secretion into the extracellular environment. Failure, overloading or malfunctioning of any of the signaling or quality control mechanisms occurring in the ER may provoke a stress condition known as âER stressâ. Accumulating evidence indicates that ER stress may dramatically perturb interactions between the cell and its environment, and contribute to the development of human diseases, ranging from metabolic diseases and cancer to neurodegenerative diseases, or impact therapeutic outcome. This book primarily focuses on the pathophysiology of ER stress. It introduces the molecular bases of ER stress, the emerging relevance of the ER-mitochondria cross-talk, the signaling pathways engaged and cellular responses to ER stress, including the adaptive Unfolded Protein Response (UPR), autophagy as well as cell death. Next the book addresses the role of ER stress in physiology and in the etiology of relevant pathological conditions, like carcinogenesis and inflammation, neurodegeneration and metabolic disease. The last chapter describes how ER stress pathways can be targeted for therapeutic benefit. Altogether, this book will provide the reader with an exhaustive view of ER stress biology and the latest insights in the role of ER stress in relevant human diseases.
  • pt. A-B, 2014 ScienceDirect
    pt. B ScienceDirect
    volume editor, P. Michael Conn.
  • 2014 CRCnetBASE
    editors, Juan A. Rosado, Pedro C. Redondo.
    The endothelial cytoskeleton : multifunctional role of the endothelial actomyosin cytoskeleton / Yuka Shimizu and Joe G.N. Garcia -- Emerging themes on pulmonary microvascular endothelial cell : microtubules and microtubule associated proteins / Cristiaan D. Ochoa, Ron Balczon, and Troy Stevens -- Endothelial actin cytoskeleton and angiogenesis / Sadiqa K. Quadri -- Adherens junctions and endothelial cytoskeleton / Younes Smani and Tarik Smani-Hajami -- Mechanical force transmission via the cytoskeleton in vascular endothelial cells / Bori Mazzag ... [et al.] -- The functional role of the microtubule/microfilament cytoskeleton in the regulation of pulmonary vascular endothelial barrier / Irina B. Alieva and Alexander D. Verin -- Membrane-cytoskeleton interactions and control of vesicle trafficking in endothelial cells / Felicia Antohe -- Cell-matrix adhesion proteins in the regulation of endothelial permeability / Jurjan Aman, Geerten P. van Nieuw Amerongen, and Victor W.M. van Hinsbergh -- Endothelial cells and the regulation of platelet function / P.C. Redondo ... [et al.].
  • 2013 Springer Protocols
    edited by Ramiro Alberio.
    Generation and Characterization of Epiblast Stem Cells from Blastocyst-stage Mouse Embryos -- Reversion of Mouse Post-Implantation Epiblast Stem Cells to a Naïve Pluripotent State by Modulation of Signalling Pathways -- Converting Mouse Epiblast Stem Cells into Mouse Embryonic Stem Cells by using Small Molecules -- Isolation and Culture of Rabbit Embryonic Stem Cells -- Goat Embryonic Stem-like Cell Derivation and Characterization -- Derivation and Culture of Canine Embryonic Stem Cells -- Isolation and Culture of Porcine Embryonic Stem Cells -- Isolation and Culture of Pig Epiblast Stem Cells -- Isolation and Culture of Bovine Embryonic Stem Cells -- Analysis of Molecular Markers for Staging Peri-gastrulating Bovine Embryos -- Chicken Embryonic Stem Cells: Establishment and Characterization -- Manipulating the Avian Epiblast and Epiblast-derived Stem Cells -- Stepwise Differentiation from Naïve State Pluripotent Stem Cells to Functional Primordial Germ Cells through an Epiblast-like State -- Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells -- Derivation of Neural Precursors from Bovine Pre-implantation Embryos.
  • 2010 Springer Protocols
    edited by Kursad Turksen.
    Isolation of adult mouse stem keratinocytes using magnetic cell sorting (MACS) / Corina Lorz [and others] -- Functional investigations of keratinocyte stem cells and progenitors at a single-cell level using multiparallel clonal microcultures / Nicolas O. Furtunel [and others] -- Growth and stratification of epithelial cells in minimal culture conditions / Federica Riva [and others] -- Matched cultures of keratinocytes and fibroblasts derived from normal and NER-deficient mouse models / Alex Pines and Claude Backendorf -- Establishment of spontaneously immortalized keratinocyte lines from wild-type and mutant mice / Julia Reichelt and Ingo Haase -- Study of epidermal differentiation in human kertinocytes cultured in autocrine conditions / Frédéric Minner, Franc̦oise Herphelin, and Yves Poumay -- Directed differentiation of human embryonic stem cells to epidermal progenitors / Christian M. Metallo [and others] -- Expression and analysis of exogenous proteins in epidermal cells / Lina Dagnino, Ernest Ho, and Wing Y. Change -- Using siRNA knockdown in HaCaT cells to study transcriptional control of epidermal proliferation potential / Julie Wells and Xing Dai -- RNA interference in keratinocytes and an organotypic model of human epidermis / Cory L. Simpson, Shin-ichiro Kojima, and Spiro Getsios -- Scanning for transcription factor binding by a variant EMSA / Igor Gurevich, Carmen Zhang, and Brian J. Aneskievich -- Chromatin immunoprecipitation for identifying transcription factor targets in kertinocytes / Kori Ortt and Satrajit Sinha -- Gene expression profiling of mouse epidermal keratinocytes / Ramón García-Escudero and Jesús M. Paramio -- Analysis of tissue-specific gene expression using laser capture microdissection / Martin Ruetze [and others] --Comprehensive transcriptional profiling of human epidermis, reconstituted epidermal equivalents, and cultured keratinocytes using DNA microarray chips / Din-Dar Lee [and others] -- Molecular profiling of the epidermis : a proteomics approach / Jianjun Shen and Susan M. Fischer -- Detection of gene expression in embryonic tissues and stratified epidermis by in situ hybridization / Maria I. Morasso -- Embryonic mammary anlagen analysis using immunolabelling of whole mounts / Heena Panchal, Olivia Wansbury, and Beatrice A. Howard -- Whole-mount assays for gene induction and barrier formation in the developing epidermis / Carolyn Byrne [and others] -- Tetracycline-regulated gene expession in transgenic mouse epidermis / Rose-Anne Romano and Satrajit Sinha -- A versatile murine 3D organotypic model to evaluate aspects of wound healing and epidermal organization / Eve Kandyba, Malcolm Hodgins, and Patricia Martin -- Optical and biochemical dissection of connexin and disease-linked connexin mutants in 3D organotypic epidermis / Stéphanie Langlois, Jared M. Churko, and Dale W. Laird -- Cytokine release in tissue-engineered epidermal equivalents after prolonged mechanical loading / Lisette H. Cornelissen [and others] -- Three-dimensional human tissue models of wounded skin / Christophe Egles, Jonathan A. Garlick, and Yulia Shamis -- In vivo transplantation of genetically modified mouse embryonic epidermis / Ana Belén Martínez-Cruz [and others] -- A transplant model for human epidermal skin regeneration / Sophie Paquet-Fifield [and others] -- Identification of epithelial stem cells in vivo and in vitro using keratin 19 and brdU / Danielle Larouche [and others] -- Isolation and culture of hair follicle pluripotent stem (hfPS) cells and their use for nerve and spinal cord regeneration / Yasuyuki Amoh and Robert M. Hoffman -- Limiting dilution analysis of murine epidermal stem cells using an in vivo regeneration assay / Lauren R. Strachan and Ruby Ghadially.
  • 2014 Springer Protocols
    edited by Kursad Turksen, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
    Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage / Igor Kogut, Dennis R. Roop, and Ganna Bilousova -- Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells / Fahad K. Kidwai, Tong Cao, and Kai Lu -- Protocol for Serial Cultivation of Epithelial Cells Without Enzymes or Chemical Compounds / Dongxia Ye and Antonio Peramo -- Growth and Differentiation of HaCaT Keratinocytes / Van G. Wilson -- Transgene Delivery to Cultured Keratinocytes Via Replication-Deficient Adenovirus Vectors / Vincent P. Ramirez and Brian J. Aneskievich -- Analyzing the Global Chromatin Structure of Keratinocytes by MNase-Seq / Jason M. Rizzo and Satrajit Sinha -- Analysis and Meta-Analysis of Transcriptional Profiling in Human Epidermis / Claudia Mimoso, Ding-Dar Lee, Jiri Zavadil, Marjana Tomic-Canic, and Miroslav Blumenberg -- Compound Screening and Transcriptional Profiling in Human Primary Keratinocytes: A Brief Guideline / Raphaela Rid, Harald Hundsberger, and Kamil Önder -- Preparation of Primary Cultures of Mouse Epidermal Keratinocytes and the Measurement of Phospholipase D Activity / Lakiea J. Bailey, Vivek Choudhary, Purnima Merai, and Wendy B. Bollag -- Lipid Rafts and Detergent-Resistant Membranes in Epithelial Keratinocytes / Kathleen P. McGuinn and Mỹ G. Mahoney -- MMP-2, 9 and TIMP-1, 2 Assays in Keratinocyte Cultures / Takashi Kobayashi -- Reactive Oxygen Species (ROS) Protection Via Cysteine Oxidation in the Epidermal Cornified Cell Envelope / Wilbert P. Vermeij and Claude Backendorf -- Modified Methods for Growing 3-D Skin Equivalents: An Update / Rebecca Lamb and Carrie A. Ambler -- A Novel Three-Dimensional Cell Culture Method to Analyze Epidermal Cell Differentiation In Vitro / Yoji Okugawa and Yohei Hirai -- Reconstruction of Normal and Pathological Human Epidermis on Polycarbonate Filter / Evelyne De Vuyst, Céline Charlier, Séverine Giltaire, Valérie De Glas, Catherine Lambert de Rouvroit, and Yves Poumay -- Methods for the Preparation of an Autologous Serum-Free Cultured Epidermis and for Autografting Applications / John J. Wille, Jeremy J. Burdge, and Jong Y. Park -- Human Keratinocyte Cultures in the Investigation of Early Steps of Human Papillomavirus Infection / Laura M. Griffin, Louis Cicchini, Tao Xu, and Dohun Pyeon -- Preparation and Delivery of 4-Hydroxy-Tamoxifen for Clonal and Polyclonal Labeling of Cells of the Surface Ectoderm, Skin, and Hair Follicle / Christine Chevalier, Jean-François Nicolas, and Anne-Cécile Petit -- Microdissection and Visualization of Individual Hair Follicles for Lineage Tracing Studies / Inês Sequeira, Emilie Legué, Suzanne Capgras, and Jean-François Nicolas -- Isolation and Characterization of a Stem Cell Side-Population from Mouse Hair Follicles / Paula L. Miliani de Marval, Sun Hye Kim, and Marcelo L. Rodriguez Puebla -- Multi-Scale Mathematical Modeling and Simulation of Cellular Dynamical Process / Shinji Nakaoka -- Erratum.
  • 2012 Springer Protocols
    edited by Scott H. Randell and M. Leslie Fulcher.
    Progress towards Drosophila epithelial cell culture / Amanda Simcox -- Culture of choroid plexus epithelial cells and in vitro model of blood-CSF barrier / Andrew D. Monnot and Wei Zheng -- Methods for culture of human corneal and conjunctival epithelia / Sandra J. Spurr-Michaud and Ilene K. Gipson -- The culture and maintenance of functional retinal pigment epithelial monolayers from adult human eye / Timothy A. Blenkinsop [and others] -- Oral and pharyngeal epithelial keratinocyte culture / Kantima Leelahavanichkul and J. Silvio Gutkind -- Primary culture of the human olfactory neuroepithelium / Nancy E. Rawson and Mehmet Hakan Ozdener -- Primary culture of mammalian taste epithelium / Mehmet Hakan Ozdener and Nancy E. Rawson -- Human nasal and tracheo-bronchial respiratory epithelial cell culture / M. Leslie Fulcher and Scott H. Randell -- Culture and differentiation of mouse tracheal epithelial cells / Yingjian You and Steven L. Brody -- Isolation and culture of alveolar epithelial type I and type II cells from rat lungs / Robert F. Gonzalez and Leland G. Dobbs -- Classical human epidermal keratinocyte cell culture / Cathy Rasmussen, Christina Thomas-Virnig, and B. Lynn Allen-Hoffmann -- Mouse epidermal keratinocyte culture / Luowei Li -- Three-dimensional culture of human breast epithelial cells : the how and the why / Pierre-Alexandre Vidi, Mina J. Bissell, and Sophie A. Lelièvre -- Three-dimensional cultures of mouse mammary epithelial cells / Rana Mroue and Mina J. Bissell -- Isolation, characterization, and reaggregate culture of thymic epithelial cells / Natalie Seach, Maree Hammett, and Ann Chidgey -- Enrichment and clonal culture of hepatic stem/progenitor cells during mouse liver development / Akihide Kamiya and Hiromitsu Nakauchi -- Long-term culture and coculture of primary rat and human hepatocytes / Maria Shulman and Yaakov Nahmias -- Tissue culture correlational study of genetic cholangiopathy of autosomal recessive polycystic kidney disease / Yasuni Nakanuma, Yasunori Sato, and Kenichi Harada -- Primary mouse small intestinal epithelial cell cultures / Toshiro Sato and Hans Clevers -- Isolation, growth, and characterization of human renal epithelial cells using traditional and 3D methods / John J. Gildea [and others] -- Human female reproductive tract epithelial cell culture / Rachel W.S. Chan [and others] -- Primary culture and propagation of human prostate epithelial cells / Birunthi Niranjan [and others] -- Urothelial cell culture : stratified urothelial sheet and three-dimensional growth of urothelial structure / Yuanyuan Zhang and Anthony Atala -- Analysis of tissue interactions in ectodermal organ culture / Pauliina M. Munne, Katja Närhi, and Frederic Michon -- Generation of human cell lines using lentiviral-mediated genetic engineering / Patrick Salmon -- Viral transformation of epithelial cells / Jennifer A. Regan and Laimonis A. Laimins.
  • 2009 Springer
    edited by Steven G. Elliott, MaryAnn Foote, and Graham Molineux.
    Erythropoiesis: an overview / B.E. Torbett and J.S. Friedman -- Regulation of endogenous erythropoietin production / D.R. Mole and P.J. Ratcliffe -- Biology of erythropoietin / G. Molineux and A.M. Sinclair -- Erythropoiesis - genetic abnormalities / J.T. Prchal and X.T. Gregg -- Studies of erythropoiesis and the discovery and cloning of recombinant human erythropoietin / M.A. Foote -- Commercial production of recombinant erythropoietins / A.S. Chuck ... [et al.] -- Biosimilar epoetins / H. Schellekens -- New molecules and formulations / S.G. Elliott -- Structural basis for the signal transduction of erythropoietin / T.D. Osslund -- Intracellular signaling by the erythropoietin receptor / H.F. Lodish ... [et al.] -- Mechanism of erythropoietin receptor activation / S.N. Constantinescu -- Pharmacokinetics of erythropoiesis-stimulating agents / S. Doshi ... [et al.] -- Use of erythropoietic stimulating agents in the setting of renal disease / D.A. Goodkin -- Abuse of recombinant erythropoietins and blood products by athletes / D.H. Catlin and C.K. Hatton -- Role and regulation of iron metabolism in erythropoiesis and disease / T.L. Arvedson and B.J. Sasu -- Nonhematopoietic effects of erythropoiesis-stimulating agents / W. Jelkmann, R. Depping and E. Metzen.
  • 2009 Springer
    Xiao-Ming Yin, Zheng Dong, editors.
  • 2013 Springer
    Robert Chunhua Zhao, editor.
    Basic Research/Mechanisms -- A Historical Overview and Concepts of Mesenchymal Stem Cells / Shihua Wang, Robert Chunhua Zhao -- Biology of MSCs Isolated from Different Tissues / Simone Pacini -- Secretome of Mesenchymal Stem Cells / Yuan Xiao ... [et al.] -- Immunomodulatory Properties of Mesenchymal Stem Cells and Related Applications / Lianming Liao, Robert Chunhua Zhao -- Mesenchymal Stem Cell Homing to Injured Tissues / Yaojiong Wu, Robert Chunhua Zhao -- Major Signaling Pathways Regulating the Proliferation and Differentiation of Mesenchymal Stem Cells / Joseph D. Lamplot ... [et al.] -- MicroRNAs in Mesenchymal Stem Cells / Mohammad T. Elnakish, Ibrahim A. Alhaider, Mahmood Khan -- Genetic Modification of MSCs for Pharmacological Screening / Jie Qin, Martin Zenke -- Control of Mesenchymal Stem Cells with Biomaterials / Sandeep M. Nalluri, Michael J. Hill, Debanjan Sarkar -- Clinical Translation -- Mesenchymal Stem Cells for Cardiovascular Disease / Wei Wu, Shuyang Zhang -- Mesenchymal Stem Cells as Therapy for Graft Versus Host Disease: What Have We Learned? / Partow Kebriaei ... [et al.] -- Mesenchymal Stem Cells for Liver Disease / Feng-chun Zhang -- Mesenchymal Stem Cells for Bone Repair / Hongwei Ouyang, Xiaohui Zou, Boon Chin Heng, Weiliang Shen -- Mesenchymal Stem Cells for Diabetes and Related Complications / Vladislav Volarevic, Majlinda Lako, Miodrag Stojkovic -- Mesenchymal Stromal Cell (MSC) Therapy for Crohn's Disease / Jignesh Dalal -- The Summary of Stroke and Its Stem Cell Therapy / Renzhi Wang ... [et al.] -- Mesenchymal Stem Cell Transplantation for Systemic Lupus Erythematosus / Lingyun Sun -- International Regulations and Guidelines Governing Stem Cell Based Products -- Considerations of Quality Control Issues for the Mesenchymal Stem Cells-Based Medicinal Products / Bao-Zhu Yuan ... [et al.] -- Regulations/Ethical Guidelines on Human Adult/Mesenchymal Stem Cell Clinical Trial and Clinical Translation / Xiaomei Zhai, Renzong Qiu.
  • 2009 ScienceDirect
    editors, Robert Lanza ... [et al.].
    Introduction to stem cells -- Basic biology/mechanisms -- Tissue and organ development -- Methods -- Applications -- Regulation and ethics.
  • 2009 Springer
    Jan Helge Solbakk, Søren Holm, Bjørn Hofmann, editors.
    Pt. 1. Research biobanking : the traditional approach -- Consent to biobank research : one size fits all? / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- What no one knows cannot hurt you : the limits of informed consent in the emerging world of biobanking / Arthur L. Caplan -- Users and uses of the biopolitics of consent : a study of DNA banks / Pascal Ducournau and Anne Cambon-Thomsen -- Information rights on the edge of ignorance / Anne Maria Skrikerud -- The dubious uniqueness of genetic information / Anne Maria Skrikerud -- Duties and rights of biobank participants : principled autonomy, consent, voluntariness and privacy / Lars Øystein Ursin -- Biobanking and disclosure of research results : addressing the tension between professional boundaries and moral intuition / Lynn G. Dressler -- Biobanks and our common good / Erik Christensen -- Trust, distrust and co-production : the relationship between research biobanks and donors / Pascal Ducournau and Roger Strand -- Scientific citizenship, benefit, and protection in population-based research / Vilhj́almur Árnason -- Pt. 2. Research Biobanking : towards a new conceptual approach -- Mapping the language of research biobanking : an analogical approach / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- The use of analogical reasoning in umbilical cord blood biobanking / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- The Alexandria plan : creating libraries for human tissue research and therapeutic use / Laurie Zoloth -- The art of biocollections / Anne Hambro Alnæs -- The health dugnard : biobank participation as the solidary pursuit of the common good / Lars Øystein Ursin and Berge Solberg -- Embodied gifting : reflections on the role of information in biobank recruitment / Klaus Hoeyer -- Conscription to biobank research? / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- Ownership rights in research biobanks : do we need a new king of 'biological property'? / Paula Lobato de Faria -- Legal challenges and strategies in the regulation of research biobanking / Elisabeth Rynning -- Annexation of life : the biopolitics of industrial biology / Jan Reinert Karlsen and Roger Strand -- In the ruins of Babel : should biobank regulations be harmonized / Jan Reinert Karlsen, Jan Helge Solbak, and Roger Strand.
  • 2013 Springer
    Fred W. Keeley, Robert P. Mecham, editors.
    The evolution of single cells into multicellular organisms was mediated, in large part, by the extracellular matrix. The proteins and glycoconjugates that make up the extracellular matrix provide structural support to cellular complexes, facilitate cell adhesion and migration, and impart mechanical properties that are important for tissue function. Each class of ECM macromolecule has evolved to incorporate distinctive properties that are defined by conserved modules that are mixed together to achieve appropriate function. This volume provides a comprehensive analysis of how the major ECM components evolved over time in order to fill their specific roles found in modern organisms. The major focus is on the structural matrix proteins, matricellular proteins, and more complex ECM structures such as basement membranes. Adhesive proteins and their receptors are also discussed.The series Biology of Extracellular Matrix is published in collaboration with the American Society for Matrix Biology.
    Also available: Print – 2013
  • 2014 Springer Protocols
    edited by Andrei I. Ivanov.
    Pharmacological inhibitors of exocytosis and endocytosis: novel bullets for old targets -- Systematic analysis of endocytosis by cellular perturbations -- Real-time detection of SNARE complex assembly with FRET using the tetracysteine system -- Profiling lysine ubiquitination by selective enrichment of ubiquitin remnant-containing peptides -- Secretion of circular proteins using sortase -- Fractionation of subcellular membrane vesicles of epithelial and non-epithelial cells by OptiPrep density gradient ultracentrifugation -- Combining pulsed SILAC labeling and click-chemistry for quantitative secretome analysis -- Probabilistic density maps to study the spatial organization of endocytosis -- Use of Kaede and Kikume green-red fusions for live cell imaging of g protein-coupled receptors -- HaloTag as a tool to investigate peroxisome dynamics in cultured mammalian cells -- SNAP-tag to monitor trafficking of membrane proteins in polarized epithelial cells -- FlAsh-palm: Super-resolution pointillist imaging with FlAsh-tetracysteine labeling -- Analysis of protein dynamics with tandem fluorescent protein timers -- Synchronization of secretory cargos trafficking in populations of cells -- Use of transmembrane fret to investigate the internalization of glycosylated proteins -- A method to rapidly induce organelle-specific molecular activities and membrane tethering -- A novel pair of split Venus fragments to detect protein-protein interactions by in vitro and in vivo bimolecular fluorescence complementation assays -- Real-time investigation of plasma membrane deformation and fusion pore expansion using polarized total internal reflection fluorescence microscopy -- Nanocones to study initial steps of endocytosis -- A novel permeabilization protocol to obtain intracellular 3d immunolabeling for electron tomography -- VIS2FIX: Rapid chemical fixation of vitreous sections for immuno-electron microscopy -- Chemical genomics: Characterizing target pathways for bioactive compounds using the endomembrane trafficking network -- Application of RNAi technology and fluorescent protein markers to study membrane traffic in c. Elegans -- Visualization of clathrin-mediated endocytosis in live drosophila egg chambers -- A novel extraction protocol to probe the role of cholesterol in synaptic vesicle recycling -- Microfluidic devices for imaging trafficking events in vivo using genetic model organisms -- The "in situ" proximity ligation assay to probe protein-protein interactions in intact tissues -- Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy -- Measurement of dynamic F-actin changes during exocytosis.
  • 2008 Springer Protocols
    [edited by] Andrei I. Ivanov.
    Vesicular trafficking : molecular tools and targets / Elena V. Vassilieva and Asma Nusrat -- Pharmacological inhibition of endocytic pathways : is it specific enough to be useful? / Andrei I. Ivanov -- In vitro assays to measure SNARE-mediated vesicle fusion / Susanne Kreye, Jörg Malsam, and Thomas H. Söllner -- Imaging Ca²⁺-triggered exocytosis of single secretory granules on plasma membrane lawns from neuroendocrine cells / Thorsten Lang -- FRAP analysis of secretory granule lipids and proteins in the sea urchin egg / Julian L. Wong and Gary M. Wessel -- A cell-free assay for endocytosis of E-cadherin / Toshiaki Sakisaka and Yoshimi Takai -- Cell-surface biotinylation to study endocytosis and recycling of occludin / Noriyuki Nishimura and Takuya Sasaki -- Fractionation of subcellular membrane vesicles of epithelial and nonepithelial cells by OptiPrep density gradient / Xuhang Li and Mark Donowitz -- Identification and characterization of interacting partners of Rab GTPases by yeast two-hybrid analyses / Mark Kail and Angelika Barnekow -- High-throughput analysis of the dynamics of recycling cell surface proteins / Roland Glovers, David E. James, and Adelle C.F. Coster -- Studying phagocytosis by live-cell scintillation proximity assay / Walter Stockinger and Axel Nohturfft -- Transcytosis of polymeric immunoglobin A in polarized Madin-Darby canine kidney cells / Asli Oztan, Christine Rondanino, and Gerard Apodaca -- The use of syntaxin chimeras to study polarized protein trafficking in epithelial cells / Martin B.A. ter Beest -- Targeting the epithelial SNARE machinery by bacterial neurotoxins / Véronique Proux-Gillardeaux and Thierry Galli -- Endocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor / Munekazu Yamakuchi ... [et al.] -- Molecular dissection of HCL secretion in gastric parietal cells using streptolysin O permeabilization / Xia Ding .. [et al.] -- Addressing membrane protein topology using the fluorescence protease protection (FPP) assay / Holger Lorenz, Dale W. Hailey, and Jennifer Lippincott-Schwartz -- Visualizing clathrin-mediated IgE receptor internalization by electron and atomic force microscopy / Alan R. Burns ... [et al.] -- Analyzing endosomes in nonsectioned cells by transmission electron microscopy / Willem Stoorvogel -- Imaging exocytosis of single insulin secretory granules with TIRF microscopy / Shinya Nagamatsu and Mica Ohara-Imaizumi -- Reconstitution of depolarization and Ca²⁺-evoked secretion in Xenopus oocytes monitored by membrane capacitance / Roy Cohen, Bernhard M. Schmitt, and Daphne Atlas -- Low-noise recording of single-vesicle capacitance steps in cell-attached patches / Vitaly Klyachko, Zhenjie Zhang, and Meyer Jackson -- Good practices in single-cell amperometry / David J. Machado, Mónica S. Montesinos, and Ricardo Borges -- Analysis of single-vesicle exocytotic events recorded by amperometry / Eugene V. Mosharov -- Application of RNAi technology and fluorescent protein markers to study membrane traffic in Caenorhabditis elegans / Dmitry Poteryaev and Anne Spang -- FM 1-43 labeling of synaptic vesicle pools at the Drosophila neuromuscular junction / Patrik Verstreken, Tomoko Ohyama, and Hugo J. Bellen -- Probing E-cadherin endocytosis by morpholino-mediated Rab5 knockdown in zebrafish / Florian Ulrich and Carl-Philipp Heisenberg -- Quantifying endocytosis in vivo using intravital two-photon microscopy / Ruben M. Sandoval and Bruce A. Molitoris.
  • 2014 Springer Protocols
    edited by Peter Thorn, School of Biomedical Sciences, University of Queensland, St. Lucia, QLD, Australia.
    Part I. Techniques in the Measurement of Exocytosis -- Solution Single-Vesicle Fusion Assay by Single-Molecule Alternating-Laser Excitation / Jae-Yeol Kim, Bong-Kyu Choi, Yeon-Kyun Shin, and Nam Ki Lee -- Imaging the Stages of Exocytosis in Epithelial Type II Pneumocytes / Thomas Haller and Paul Dietl -- Carbon-Fiber Amperometry in the Study of Exocytosis / Michael D. Duffield, Ravinarayan Raghupathi, and Damien J. Keating -- Imaging of Insulin Exocytosis from Pancreatic Beta Cells / Mica Ohara-Imaizumi, Kyota Aoyagi, and Shinya Nagamatsu -- Functional, Quantitative, and Super-Resolution Imaging and Spectroscopic Approaches for Studying Exocytosis / Rory R. Duncan and Colin Rickman -- Electrophysiologic Measurements of Membrane Capacitance in Hormone-Secreting Cells / Boštjan Rituper and Robert Zorec. Part II. Model Systems for the Study of Exocytosis -- Measuring Exocytosis in Endocrine Tissue Slices / Maša Skelin Klemen, Jurij Dolenšek, Andraž Stožer, and Marjan Slak Rupnik -- Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Live Rodents / Oleg Milberg, Natalie Porat-Shliom, Muhibullah Tora, Laura Parente, Andrius Masedunskas, and Roberto Weigert -- Rapid Analysis of Synaptic Vesicle Endocytosis in Synaptosomes / James A. Daniel and Phillip J. Robinson -- Isolated Neurohypophysial Terminals: Model for Depolarization-Secretion Coupling / José R. Lemos, James McNally, Edward Custer, Adolfo Cuadra, Hector Marrero, and Dixon Woodbury -- The Sea Urchin Egg and Cortical Vesicles as Model Systems to Dissect the Fast, Ca²-Triggered Steps of Regulated Exocytosis / Prabhodh S. Abbineni, Elise P. Wright, Tatiana P. Rogasevskaia, Murray C. Killingsworth, Chandra S. Malladi, and Jens R. Coorssen.
  • 2008 Springer
    N. Taniguchi ... [et al.], (eds.).
  • 2010 Springer
    Evgenia Gerasimovskaya, Elzbieta Kaczmarek, editors.
  • 2014 Springer Protocols
    edited by Jennie B. Leach, Elizabeth M. Powell.
    This volume provides comprehensive procedures for analyzing the extracellular matrix in native, injured, and engineered neural tissues. Divided into four parts, each focusing on different aspects of the extracellular matrix and the nervous system, Extracellular Matrix covers methods to analyze native tissue, in vitro models for investigating cell-extracellular matrix interactions in a variety of contexts, protocols to investigate the role of the extracellular matrix in nervous system injury, degeneration, and regeneration, as well as therapeutics and engineered systems. Each chapter is written by leading experts and presents established protocols in a concise format, encompassing current technologies as well as methods developed over years of research. Beginning with an introduction to the method, chapters continue with a listing of the materials and equipment, step-by-step protocols, and a Notes section with troubleshooting tips, supplemental details and alternative approaches, as well as a list of references for further information.
  • 2011 Springer
    Robert P. Mecham, editor.
    An overview of extracellular matrix structure and function / Jürgen Engel and Matthias Chiquet -- Fibronectin and other adhesive glycoproteins / Jielin Xu and Deane Mosher -- Collagens, suprastructures, and collagen fibril assembly / David E. Birk and Peter Brückner -- Basement membranes / Jeffrey H. Miner -- Hyaluronan and the aggregating proteoglycans / Thomas N. Wight, Bryan P. Toole, and Vincent C. Hascall -- Small leucine-rich proteoglycans / Renato V. Iozzo ... [et al.] -- Microfibrils and fibrillin / Dirk Hubmacher and Dieter P. Reinhardt -- Elastin / Beth A. Kozel, Robert P. Mecham, and Joel Rosenbloom -- Lysyl oxidase and lysyl oxidase-like enzymes / Herbert M. Kagan and Faina Ryvkin -- The fibulins / Marion A. Cooley and W. Scott Argraves -- Matricellular proteins / David D. Roberts and Lester F. Lau.
    Also available: Print – 2011
  • 2011 Springer
    William C. Parks, Robert P. Mecham, editors.
    Matrix metalloproteinases and their inhibitors in turnover and degradation of extracellular matrix / Sean E. Gill and William C. Parks -- Role of cysteine cathepsins in extracellular proteolysis / Dieter Brömme and Susan Wilson -- Cooperation between proteolysis and endocytosis in collagen turnover / Thomas H. Bugge and Niels Behrendt -- Pericellular proteolysis / Judith S. Bond, Timothy R. Keiffer, and Qi Sun -- Triple helicase activity and the structural basis of collagenolysis / Hideaki Nagase and Robert Visse -- Structural basis of extracellular matrix interactions with matrix metalloproteinases / Steven R. Van Doren -- The role of matrix metalloproteinases in cellular invasion and metastasis / Elena I. Deryugina and James P. Quigley -- Degradation of bone and the role of osteoclasts, bone lining cells and osteocytes / Vincent Everts and Paul Saftig -- Consequences of elastolysis / A. McGarry Houghton, Majd Mouded, and Steven D. Shapiro.
    Also available: Print – 2011
  • 2013 Springer
    Douglas W. DeSimone, Robert P. Mecham, editors.
    Cells in the developing embryo depend on signals from the extracellular environment to help guide their differentiation. An important mediator in this process is the extracellular matrix - secreted macromolecules that interact to form large protein networks outside the cell. During development, the extracellular matrix serves to separate adjacent cell groups, participates in establishing morphogenic gradients, and, through its ability to interact directly will cell-surface receptors, provides developmental clocks and positional information. This volume discusses how the extracellular matrix influences fundamental developmental processes and how model systems can be used to elucidate ECM function. The topics addressed range from how ECM influences early development as well as repair processes in the adult that recapitulate developmental pathways.The series Biology of Extracellular Matrix is published in collaboration with the American Society for Matrix Biology.
    Also available: Print – 2013
  • 2014 CRCnetBASE
    edited by Paul Harrison, Chris Gardiner, Ian L. Sargent.
    1. Overview of extracellular vesicles in health and disease / Rienk Nieuwland, Edwin van der Pol, and Augueste Sturk -- 2. The biogenesis of exosomes / D. Michiel Pegtel -- 3. Extracellular vesicle-mediated epigenetic reprogramming of cells / Giovanni Camussi, Federica Collino, and Maria C. Deregibus -- 4. An overview of novel and conventional methods to detect extracellular vesicles / Edwin van der Pol, Ton G. van Leeuwen, and Rienk Nieuwland -- 5. Preanalytical variables / Romaric Lacroix, Coralie Judicone, Stéphane Robert, Laurent Arnaud, Florence Sabatier, and Françoise Dignat-George -- 6. Procoagulant assays / Barry J. Woodhams -- 7. Capture-based assays for extracellular vesicles within the blood / Jean Amiral -- 8. Flow cytometry / Romaric Lacroix, Stephane Robert, Florence Sabatier, and Francoise Dignat-George -- 9. Atomic force microscopy measurement of extracellular vesicles derived from plasma / Yuana Yuana and Susanne Osanto -- 10. Light-scattering methods to characterize extracellular vesicles / Don A. Gabriel, Sam Glover, Regina Bowling, and Nigel Key -- 11. Nanoparticle tracking analysis / Chris Gardiner and Rebecca Dragovic -- 12. Extracellular vesicle proteomic analysis / Andrés F. Parguiña and Ángel García -- 13. Microvesicles and exosomes in cancer / Rebecca Karp and Jeffrey Zwicker -- 14. Extracellular vesicles and tissue factor / Pierre-Emmanuel Rautou and Nigel Mackman -- 15. Extracellular vesicles in normal pregnancy and pre-eclampsia / Ian L. Sargent, Rebecca A. Dragovic, Dionne S. Tannetta, and Christopher W. Redman -- 16. Extracellular vesicles in cardiovascular disease / Anne-Clémence Vion and Chantal M. Boulanger -- 17. Extracellular vesicles in immunology / Els J. van der Vlist, Esther N.M. Nolte-'t Hoen, and Marca H.M. Wauben.
  • 2012 Wiley
    Robert Brown.
  • 2008 Springer
    Ali Mobasheri [and others].
    Articular cartilage is a unique and highly specialized avascular connective tissue in which the availability of oxygen and glucose is significantly lower than synovial fluid and plasma. Glucose is an essential source of energy during embryonic growth and fetal development and is vital for mesenchymal cell differentiation, chondrogenesis and skeletal morphogenesis. Glucose is an important metabolic fuel for differentiated chondrocytes during post-natal development and in adult articular cartilage and is a common structural precursor for the synthesis of extracellular matrix glycosaminoglycans.
  • 2006 Springer
    Gabrijela Kocjan.
    Introduction and historical perspective -- FNAC technique and slide preparation -- Diagnostic interpretation of FNAC material -- Diagnostic dilemmas in FNAC practice: cystic lesions -- Diagnostic dilemmas in FNAC practice: lymphoid infiltrates -- Diagnostic dilemmas in FNAC practice: metastatic tumours -- Diagnostic dilemmas in FNAC cytology: small round cell tumours -- Diagnostic dilemmas in FNAC cytology: soft-tissue lesions -- Diagnostic dilemmas in FNAC cytology: difficult breast lesions -- Principles of safe practice: the role of FNAC in clinical management -- Principles of safe FNAC practice: FNAC versus core biopsy -- Principles of safe FNAC practice: medicolegal issues.
  • 2007 Springer
    edited by Marion G. Macey.
    Principles of flow cytometry / Marion G. Macey -- Cell preparation / Desmond A. McCarthy -- Fluorochromes and fluorescence / Desmond A. McCarthy -- Quality control in flow cytometry / David Barnett and John T. Reilly -- Experimental design, data analysis, and fluorescence quantitation / Mark Lowdell -- Apoptosis detection by flow cytometry / Paul Allen and Derek Davies -- DNA analysis by flow cytometry / Derek Davies and Paul Allen -- Immunological studies of human cells / Ulrika Johansson -- Calcium: cytoplasmic, mitochondrial, endoplasmic reticulum, and flux measurements / Gary Warnes and Marion G. Macey -- Further functional studies / Marion G. Macey -- Cell sorting by flow cytometry / Derek Davies.
    Also available: Print – 2007
  • 2014 Springer
    Christopher P. Toseland, Natalia Fili, editors.
    This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.
  • 2014 CRCnetBASE
    edited by Richard N. Day, Michael W. Davidson.
    Introduction to fluorescent proteins / Marc Zimmer -- Lessons learned from the structure of aequorea victoria fluorescent proteins / S. James Remington -- Optimization of fluorescent proteins / Mark A. Rizzo -- Development of new colors from coral fluorescent proteins / Nathan C. Shaner -- Red fluorescent proteins : multipurpose markers for live cell imaging / Jörg Wiedenmann and G. Ulrich Nienhaus -- Optical highlighter photophysical properties / George H. Patterson -- Far red and near infrared fluorescent proteins / Jun Chu, Yan Xing, and Michael Z. Lin -- Genetically-encoded fluorescent proteins and FRAP / James G. McNally -- Optical highlighters : applications to cell biology / Malte Renz and Jennifer Lippincott-Schwartz -- Optogenetic tools derived from plant photoreceptors / John M. Christie -- Fluorescent proteins for FRET : monitoring protein interactions in living cells / Richard N. Day -- Superresolution techniques / Michael W. Davidson -- The in vivo imaging revolution made by fluorescent proteins / Robert Hoffman.
  • 2008 ScienceDirect
    edited by Kevin F. Sullivan.
    Also available: Print – 2008
  • 2011 Springer
    Andreas Wyttenbach, Vincent O'Connor, editors.
    Folding for the Synapse addresses the current view on how protein folding and misfolding, controlled by molecular chaperones, contribute to synapse function and dysfunction. Molecular chaperones have been studied in relation to de novo protein folding, but there is increasing awareness that chaperone function is required for the regulation of protein dynamics when functioning physiologically as an isolated moiety or part of a protein complex. This book will introduce both important concepts of folding machineries and give examples of the biological relevance of further chaperone functions.
  • 2011 Springer
    Katharina Spanel-Borowski.
    The cyclic ovary can be seen as a site of tissue damage, repair and precisely controlled tissue homeostasis, as long ovulation and luteolysis can be compared with acute and chronic inflammation. Innate immunity appears to be a powerful force in the endocrine system, representing a novel concept. In this monograph, remarkable evidence is given for the immune-privileged ovary being an implant on the chicken chorioallantoic membrane with an areactive mesenchyme. Mild to severe tissue damages due to follicular atresia, follicular rupture, or intraovarian oocyte release do no lasting harm. The most exciting part relates to the analysis of cytokeratin-positive (CK<sup>+</sup>) cells, comparing the fate mapping of this cell type from the fetal ovary to the adult organ. Findings on toll-like receptor 4 regulation and interferon-gamma-dependent positive effects indicate that CK<sup>+</sup> cells from human preovulatory follicles and bovine corpora lutea have similarities with nonlymphoid dendritic cells, a discovery that has the makings of a top story in basic and clinical research on the ovary.
  • 2014 Springer Protocols
    edited by Juan C. Stockert, Jesús Espada, Alfonso Blázquez-Castro.
    Predictive binding geometry of ligands to DNA minor groove : isohelicity and hydrogen-bonding pattern / Juan C. Stockert -- Using microchip gel electrophoresis to probe DNA-drug binding interactions / Nan Shi and Victor M. Ugaz -- Identifying different types of chromatin using Giemsa staining / Juan C. Stockert, Alfonso Blázquez-Castro, and Richard W. Horobin -- Analysis of DNA damage and repair by comet fluorescence in situ hybridization (comet-FISH) / Michael Glei and Wiebke Schlörmann -- Alkaline nuclear dispersion assays for the determination of DNA damage at the single cell level / Piero Sestili and Carmela Fimognari -- Polarization microscopy of extended chromatin fibers / Maria Luiza S. Mello and Benedicto de Campos Vidal -- DNA labeling in vivo : quantification of epidermal stem cell chromatin content in whole mouse hair follicles using Fiji image processing software / Elisa Carrasco, María I. Calvo, and Jesús Espada -- Historical overview of bromo-substituted DNA and sister chromatid differentiation / Roberto Mezzanotte and Mariella Nieddu -- Image analysis of chromatin remodelling / Benedicto de Campos Vidal, Marina B. Felisbino, and Maria Luiza S. Mello -- FISH methods in cytogenetic studies / Miguel Pita, Juan Orellana, Paloma Martínez-Rodríguez, Ángel Martínez-Ramírez, Begoña Fernández-Calvín, and José L. Bella -- Ultrastructural and immunofluorescent methods for the study of the XY body as a biomarker / Roberta B. Sciurano and Alberto J. Solari -- Atomic force microscopy for analyzing metaphase chromosomes : comparison of AFM images with fluorescence labeling images of banding patterns / Osamu Hoshi and Tatsuo Ushiki -- Selective detection of phagocytic phase of apoptosis in fixed tissue sections / Vladimir V. Didenko -- Selective transport of cationized fluorescent topoisomerase into nuclei of live cells for DNA damage studies / Candace L. Minchew and Vladimir V. Didenko -- Visualization and interpretation of eukaryotic DNA replication intermediates in vivo by electron microscopy / Kai J. Neelsen, Arnab Ray Chaudhuri, Cindy Follonier, Raquel Herrador, and Massimo Lopes -- Combined bidimensional electrophoresis and electron microscopy to study specific plasmid DNA replication intermediates in human cells / Cindy Follonier and Massimo Lopes -- Standard DNA methylation analysis in mouse epidermis : bisulfite sequencing, methylation-specific PCR, and 5-methyl-cytosine (5mC) immunological detection / Jesús Espada, Elisa Carrasco, and María I. Calvo -- Methyl-combing : single-molecule analysis of DNA methylation on stretched DNA fibers / Attila Németh -- Investigating 5-hydroxymethylcytosine (5hmC) : the state of the art / Colm E. Nestor, James P. Reddington, Mikael Benson, and Richard R. Meehan -- Hydroxymethylated DNA immunoprecipitation (hmeDIP) / Colm E. Nestor and Richard R. Meehan -- Microscale thermophoresis for the assessment of nuclear protein-binding affinities / Wei Zhang, Stefan Duhr, Philipp Baaske, and Ernest Laue -- Analysis of histone posttranslational modifications from nucleolus-associated chromatin by mass spectrometry / Stefan Dillinger, Ana Villar Garea, Rainer Deutzmann, and Attila Németh -- Salt-urea, sulfopropyl-sepharose, and covalent chromatography methods for histone isolation and fractionation / Pedro Rodriguez-Collazo -- Chromatin immunoprecipitation / Javier Rodríguez-Ubreva and Esteban Ballestar -- Analysis of chromatin composition of repetitive sequences : the ChIP-Chop assay / Raffaella Santoro -- Purification of specific chromatin domains from single- copy gene loci in Saccharomyces cerevisiae / Stephan Hamperl, Christopher R. Brown, Jorge Perez-Fernandez, Katharina Huber, Manuel Wittner, Virginia Babl, Ulrike Stöckl, Hinrich Boeger, Herbert Tschochner, Philipp Milkereit, and Joachim Griesenbeck -- Deep sequencing of small chromatin-associated RNA : isolation and library preparation / Sarah Daniela Diermeier, Thomas Schubert, and Gernot Längst -- Deep sequencing of small chromatin-associated RNA : bioinformatic analysis / Sarah Daniela Diermeier and Gernot Längst.
  • 2010 Springer
    Margit Pavelka, Jürgen Roth.
  • 2007 Springer
    by Rana S. Hoda, Syed A. Hoda ; foreword by Prabodh K. Gupta.
    A short dictionary of the Pap test -- Analogies in the Pap test : "Of corn flakes and raisins" -- Basic cytology principles -- Basic anatomy and cytology of the female genital tract -- The Bethesda system -- Liquid-based cytology -- Physiological cytology -- Reactive changes and organisms -- Human papillomavirus in cervical carcinogenesis -- Atypical squamous cells -- Low-grade squamous intra-epithelial lesion -- High-grade squamous intra-epithelial lesion -- Invasive squamous carcinoma -- Endocervical lesions -- Endometrial lesions -- Metastatic tumors -- Therapy-related changes -- Artifacts, contaminants, and incidental findings -- Screening and management guidelines of Pap test and newer techniques.
  • 2012 Springer
    edited by Monika Fuxreiter, Peter Tompa.
    Fuzzy complexes : a more stochastic view of protein function / Monika Fuxreiter and Peter Tompa -- Dynamic fuzziness during linker histone action / Steven J. McBryant and Jeffrey C. Hansen -- Intrinsic protein flexibility in regulation of cell proliferation : advantages for signaling and opportunities for novel therapeutics / Ariele Viacava Follis, Charles A. Galea, and Richard W. Kriwacki -- Interplay between protein order, disorder and oligomericity in receptor signaling / Alexander B. Sigalov -- Consequences of fuzziness in the NFB/IB interaction / Elizabeth A. Komives -- Roles for intrinsic disorder and ̂fuzziness in generating context specific function in ultrabithorax : a hox transcription factor / Sarah E. Bondos and Hao Ching Hsiao -- Molecular recognition by the EWS transcriptional activation domain / Kevin A.W. Lee -- The measles virus NTAILXD complex : an illustrative example of fuzziness / Sonia Longhi -- Fuzziness in the core of the human pathogenic viruses HCV and HIV / Roland Ivanyi Nagy and Jean Luc Darlix -- Structural disorder and protein elasticity / Sarah Rauscher and Røgis Pomès -- "Fuzziness" in the cellular interactome: a historical perspective / G. Rickey Welch.
    Also available: Print – 2012
  • 2016 ScienceDirect
    edited by Arun K. Shukla.
  • 2013 Springer
    Tiziana A.L. Brevini, Georgia Pennarossa.
    This Brief offers a concise, handy overview of the main concepts related to Embryology, revisited through the novel concepts that are applied daily in stem cell research and cell therapy oriented investigations. It is based on three main areas: -The process involved in female gamete differentiation and maturation. The main aspects related to cell biology will be covered and an overview of the epigenetic regulation of gametogenesis will be presented. -Early stages of embryo development with a careful analysis of the regulatory mechanisms driving cleavage, polarization and genome activation. -Stem cell and gametogenesis. The use of the oocyte as a possible source for the derivation of stem cell lines is discussed and depicted as a powerful tool to investigate oocyte potency and asymmetric imprinting. The potential biological implications are evaluated and use of stem cells to derive oocytes is presented.
  • 2005 Springer
    Elke Winterhager (ed.).
  • 2012 Springer
    Anton Hermann, Guzel F. Sitdikova, Thomas M. Weiger, editors.
    "The gases termed gasotransmitters play an increasingly important role in understanding how signalling into and between cells is modulated and fine-tuned. The advent of gasotransmitters has profoundly changed our way of thinking about biosynthesis, liberation, storage and action mechanisms in cellular signaling. In recent years an impressive amount of new data, distributed throughout the existing literature, has been generated. For this book the editors have recruited distinguished colleagues in the field to summarize and review important biological, pharmacological and medical functions and their implications, as well as methods for the detection of gasotransmitters."--P. [4] of cover.
  • 2006 Springer
    edited by Jan A. Nolta.
    Also available: Print – 2006
  • 2009 Springer Protocols
    edited by Christopher Baum.
    Immunomagnetic enrichment of human and mouse hematopoietic stem cells for gene therapy applications / Guillermo Guenechea, Jose C. Segovia, and Juan A. Bueren -- Isolation of human and mouse hematopoietic stem cells / Yuk Yin Ng ... [et al.] -- Murine hematopoietic stem cell transduction using retroviral vectors / Ute Modlich ... [et al.] -- Genetic modification of human hematopoietic cells: preclinical optimization of oncoretroviral-mediated gene transfer for clinical trials / Tulin Budak-Alpdogan and Isabelle Rivière -- Short-term culture of human CD34⁺ cells for lentiviral gene transfer / Francesca Santoni de Sio and Luigi Naldini -- T cell culture for gammaretroviral transfer / Sebastian Newrzela, Gunda Brandenburg, and Dorothee von Laer -- Retroviral transduction of murine primary T lymphocytes / James Lee, Michel Sadelain, and Renier Brentjens -- Lentiviral vector gene trasfer into human T cells / Els Verhoeyen, Caroline Costa, and Francois-Loic Cosset -- DNA transposons for modification of human primary T lymphocytes / Xin Huang ... [et al.] -- Retroviral gene transfer into primary human natural killer cells / Evran Alici, Tolga Sutlu, and M. Sirac Dilber -- Lentiviral vector-mediated genetic programming of mouse and human dendritic cells / Renata Stripecke -- In situ (in vivo) gene transfer into murine bone marrow stem cells / Dao Pan -- In vivo and ex vivo gene transfer in thymocytes and thymocyte precursors / Oumeya Adjali ... [et al.] -- Design and production of retro- and lentiviral vectors for gene expression in hematopoietic cells / Axel Schambach, William P. Swaney, and Johannes C.M. van der Loo -- Knock-down of gene expression in hematopoietic cells / Michaela Scherr, Letizia Venturiani, and Matthias Eder -- The use of retroviral vectors for tet-regulated gene expression in cell populations / Rainer Löw -- Detection of replication competent retrovirus and lentivirus / Lakshmi Sastry and Kenneth Cornetta -- Release testing of retroviral vectors and gene-modified cells / Diana Nordling, Anne Kaiser, and Lilith Reeves -- Copy number determination of genetically-modificed hematopoietic stem cells / Todd Schuesler ... [et al.] -- Tissue procurement for molecular studies using laser-assisted microdissection / Ulrich Lehmann and Hans Kreipe -- Leukemia diagnosis in murine bone marrow transplantation models / Zhixiong Li, Ute Modlich, and Anjali Mishra -- Humanized mouse models to study the human haematopoietic stem cell compartment / Dominique Bonnet -- Canine models of gene-modified hematopoiesis / Brian C. Beard and Hans-Peter Kiem -- Detection of retroviral integration sites by linear amplification-mediated PCR and tracking of individual integration clones in different samples / Manfred Schmidt ... [et al.] -- Retroviral insertion site analysis in dominant haematopoietic clones / Olga S. Kustikova, Ute Modlich, and Boris Fehse -- Tracking gene-modified T cells in vivo / Alessandra Recchia and Fulvio Mavilio -- DNA microarray studies of hematopoietic subpopulations / Karin Pike-Overzet ... [et al.] -- Quantification of genomic mutations in murine hematopoietic cells / Hartmut Geiger ... [et al.] -- Proteomics studies after hematopoietic stem cell transplantation / Eva M. Weissinger, Petra Zürbig, and Arnold Ganser -- Spectral karyotyping and fluorescence in situ hybridization of murine cells / Cornelia Rudolph and Brigitte Schlegelberger -- Database setup for preclinical studies of gene-modified hematopoiesis / Brenden Balcik, Elke Grassman, and Lilith Reeves -- The US and EU regulatory perspectives on the clinical use of hematopoietic stem/progenitor cells genetically modified ex vivo by retroviral vectors / Carolyn A. Wilson and Klaus Cichutek.
  • 2007 Springer
    volume editor, Dirk-Henner Lankenau.
    From microorganisms to humans, this volume provides an interdisciplinary overview of how genome integrity is maintained. It begins with DNA replication and continues with replicative DNA repair and pleiotropic protein interactions. It also reviews the cellular responses to radiation and genotoxic stress affecting whole genomes.
  • 2013 Springer
    Tim Schedl, editor.
    Also available: Print – 2013
  • 2012 Springer Protocols
    edited by Wai-Yee Chan, Le Ann Blomberg.
    Isolation of fetal gonads from embryos of timed-pregnant mice for morphological and molecular studies -- Neonatal testicular gonocytes isolation and processing for immunocytochemical analysis -- Isolation of undifferentiated and early differentiating type a spermatogonia from pou5f1-GFP reporter mice -- Isolation of human male germ-line stem cells using enzymatic digestion and magnetic-activated cell sorting -- Isolation and purification of murine male germ cells -- Preparation of enriched mouse syncitia-free pachytene spermatocyte cell suspensions -- Revealing the transcriptome landscape of mouse spermatogonial cells by tiling microarray -- Biochemical characterization of a testis-predominant isoform of n-[alpha] acetyltransferase -- Identification of novel long noncoding RNA transcripts in male germ cells -- Methylation profiling using methylated DNA immunoprecipitation and tiling array hybridization -- Spermatogenesis in cryptorchidism -- In vitro culture of fetal ovaries: A model to study factors regulating early follicular development -- Microspread ovarian cell preparations for the analysis of meiotic prophase progression in oocytes with improved recovery by cytospin centrifugation -- In vitro maturation (IVM) of porcine oocytes -- Experimental approaches to the study of human primordial germ cells -- Investigating the origins of somatic cell populations in the perinatal mouse ovaries using genetic lineage tracing and immunohistochemistry -- DNA methylation analysis of germ cells by using bisulfite-based sequencing methods -- Gene expression in mouse oocytes by RNA-Seq.
  • 2008 Springer Protocols
    edited by Steven X. Hou, Shree Ram Singh.
    pt. I. Identification and regulation of germline stem cells in model organisms -- The development of germline stem cells in Drosophila / David A. Dansereau and Paul Lasko -- Analysis of the C. elegans germline stem cell region / Sarah L. Crittenden and Judith Kimble -- Immunohistological techniques for studying the Drosophila male germline stem cell / Shree Ram Singh and Steven X. Hou -- Genetic tools used for cell lineage tracing and gene manipulation in Drosophila germline stem cells / Wei Liu and Steven X. Hou -- Structural polarity and dynamics of male germline stem cells in an insect (milkweed bug Oncopeltus fasciatus) / David C. Dorn and August Dorn -- High-resolution light microscopic characterization of spermatogonia / Hélio Chiarini-Garcia and Marvin L. Meistrich -- Identification and characterization of spermatogonial subtypes and their expansion in whole mounts and tissue sections from primate testes / Jens Ehmcke and Stefan Schlatt -- Epigenetic control in male germ cells : a transillumination-assisted microdissection method for the analysis of developmentally regulated events / Durga Prasad Mishra and Paolo Sassone-Corsi -- GDNF maintains mouse spermatogonial stem cells in vivo and in vitro / Hannu Sariola and Tiina Immonen -- g pt. II. In vitro culture and application of germline stem cells -- Ectopic grafting of mammalian testis tissue into mouse hosts / Ina Dobrinski and Rahul Rathi -- Spermatogonial stem cell transplantation, testicular function, and restoration of male fertility in mice / Derek J. McLean -- Isolating highly pure rat spermatogonial stem cells in culture / F. Kent Hamra ... [et al.] -- Deriving mouse spermatogonial stem cell lines / Ilaria Falciatori ... [et al.] -- Production of knockdown rats by lentiviral transduction of embryos with short hairpin RNA transgenes / Christina Tenenhaus Dann and David L. Garbers -- Testicular germ cell tumors in mice : new ways to study a genetically complex trait / Jason D. Heaney and Joseph H. Nadeau -- Study origin of germ cells and formation of new primary follicles in adult human and rat ovaries / Antonin Bukovsky ... [et al.].
  • 2015 Springer
    Rosemary H. Tambouret, David C. Wilbur.
    This volume provides a compact, concise and up-to-date resource for pathologists and clinicians who are involved in women's health. The book summarizes the incidence, epidemiology and risk factors for cervical adenocarcinoma, while reviewing the normal histology of the cervix and glandular lesions of the cervix. The cytological appearance of glandular lesions of the cervix and their mimics is amply illustrated and discussed. Management of glandular lesions is also outlined. Written by authorities in the field, Glandular Lesions of the Uterine Cervix: Cytopathology with Histologic Correlates is of great value to cytopathologists, surgical pathologists, gynecologic oncologists and surgeons.
  • 2009 Springer
    Henry deF. Webster, Karl E. Åström.
    Gliogenesis -- Astroglia -- Oligodendroglia -- Microglia -- Ependymal cells -- Tanycytes -- Schwann cells.
  • 2013 Springer
    Jolanta Barańska, editor.
    Also available: Print – 2013
  • 2011 Springer
    Mauro S.G. Pavão, editor.
    Heparan sulfate proteoglycan in inflammation and angiogenesis / Lianchun Wang -- Heparan sulfate proteoglycans in infection / Allison H. Bartlett and Pyong Woo Park -- Glycans in cancer / Lubor Borsig -- Glycosaminoglycans in atherosclerosis and thrombosis / C.P. Vicente, J.A.P. Godoy, and C.C. Werneck -- Glycans in embryonic stem cells / Claire Pickford ... [et al.] -- Heparan sulfate proteoglycans in cancer therapy / Ezeddin Salem Gassar, Sherif A. Ibrahim, and Martin Götte -- Structure and biological activities of glycosaminoglycan analogs from marine invertebrates : new therapeutic agents? / Eliene O. Kozlowski ... [et al.]
    Also available: Print – 2011
  • 2009 Springer
    E. Nigel Wardle.
  • 2010 ScienceDirect
    edited by Paul M. Wassarman and Philippe Soriano.
    Also available: Print – 2010
  • 2010 ScienceDirect
    edited by Paul M. Wassarman and Philippe M. Soriano.
    Also available: Print – 2010
  • 2005 NAP
    Committee on Guidelines for Human Embryonic Stem Cell Research, Board on Life Sciences, Division on Earth and Life Studies, Board on Health Sciences Policy, Institute of Medicine, National Research Council and Institute of Medicine of the National Academies.
  • 2005 Springer
    K. Morioka.
  • 2010 ScienceDirect
    editors-in-chief, Ralph A. Bradshaw, Edward A. Dennis.
  • 2012 CRCnetBASE
    edited by Keiko Hirose, Linda Amos.
    "This book draws together recent data on both cytoplasmic and flagellar dyneins and the proteins they interact with, to give readers a clear picture of what is currently known about the structure and mechanics of these remarkable macro-molecular machines. Each chapter is written by active researchers, with a focus on currently used biophysical, biochemical, and cell biological methods. This is a useful handbook for frontline researchers as well as a textbook for advanced students."--P. [4] of cover.
  • 2011 CRCnetBASE
    edited by Leland J. Cseke, Ara Kirakosyan, Peter B. Kaufman, Margaret V. Westfall.
    "Reflecting the milestones in the field that have occurred since the publication of the first edition, this book provides an updated tool kit that incorporates conventional as well as modern approaches to tackling biological and medicinal research in the post genomic era. The authors have evaluated, revised, and sometimes replaced all protocols with more efficient, reliable, or simpler ones. The book is also completely reorganized and covers topics of modern "omics" approaches, including genomics, proteomics, and metabolomics. This is followed coverage of the Manipulation of Biological Systems, including RNAi, and the use of microscopy"--Provided by publisher.
  • 2015 Springer
    Fan Lin, Jeffrey Prichard, editors.
    In a conceptually current, quick-reference, Question & Answer format, the second edition of Handbook of Practical Immunohistochemistry: Frequently Asked Questions continues to provide a comprehensive and yet concise state-of-the-art overview of the major issues specific to the field of immunohistochemistry. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 10,000 tumors and normal tissue to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. ℗ℓ Fully revised and updated from the first edition, the new features of the second edition include over 200 additional questions or revised questions with an IHC panel to answer each question; over 250 new color photos and illustrations; over 20 new useful biomarkers; hundreds of new references; several new chapters to cover phosphoproteins, rabbit monoclonal antibodies, multiplex IHC stains, overview of predictive biomarkers, and integration of IHC into molecular pathology; many new coauthors who are international experts in a related field; many updated IHC panels using Geisinger IHC data collected from over 10,000 tumors and normal tissues; and updated appendices containing detailed antibody information for both manual and automated staining procedures. ℗ℓ Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, Second Edition will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.
  • 2011 Springer
    Fan Lin, Jeffrey Prichard, editors ; Haiyan Liu, Myra Wilkerson, Conrad Schuerch, assoc. editors.
    Quality management and regulation / Jeffrey Prichard -- Technique and troubleshooting of antibody testing / Fan Lin and Jianhui Shi -- Overview of automated immunohistochemistry / Jeffrey Prichard, Angela Bitting, and Joe Myers -- Automated staining : Dako perspective / Ole F. Rasmussen and Andreas Schønau -- Automated staining : Ventana perspective / Angela Sattler -- Tissue microarray / Myra Wilkerson and Erin Powell -- Unknown primary/undifferentiated neoplasms in surgical and cytologic specimens / Fan Lin and Haiyan Liu -- Exfoliative cytopathology / Steven Meschter and Jan F. Silverman -- Predictive markers of breast cancer : ER, PR, and HER2 / Jeffrey Prichard, David Hicks, and Elizabeth Hammond -- Central and peripheral nerve system tumors / Hueizhi (Hope) Wu, Conrad Schuerch, and Douglas C. Miller -- Thyroid and parathyroid gland / Haiyan Liu, Fan Lin, and Ronald A. DeLellis -- Adrenal gland / Hanna G. Kaspar -- Salivary gland and other head and neck structures / Conrad Schuerch -- Lung, pleura, and mediastinum / Kai Zhang and Phillip Cagle -- Breast / Haiyan Liu -- Uterus / Hanna G. Kaspar -- Ovary / Jeffrey Prichard, Haiyan Liu, and Myra Wilkerson -- Prostate gland / Haiyan Liu, Fan Lin, and Qihui (Jim) Zhai -- Urinary bladder / Myra Wilkerson -- Kidney / Fan Lin and Ximing J. Yang -- Testis and paratesticular tissues / Myra Wilkerson -- Pancreas and ampulla / Fan Lin and Hanlin L. Wang -- Liver, bile ducts, and gallbladder / Jeffrey Prichard and Fan Lin -- Upper gastrointestinal tract / Jinhong Li and Fan Lin -- Lower gastrointestinal tract and microsatellite instability / Jinhong Li and Fan Lin -- Soft tissue and bone tumors / Shaobo Zhu and Markku Miettinen -- Lymph node / Xiaohong (Mary) Zhang and Nadine S. Aguilera -- Bone marrow / R. Patrick Dorion and Xiaohong (Mary) Zhang -- Infectious diseases / Dirk M. Elston, Lawrence E. Gibson, and Heinz Kutzner -- Skin / Tammie Ferringer -- Application of direct immunofluorescence for the skin and mucosal biopsies : a practical review / William B. Tyler -- In situ hybridization in surgical and cytologic specimens / Hong Yin and Barbara Paynton.
  • 2004 ScienceDirect
    editors, Robert Lanza, John Gearhart, Helen Blau ... [et al.].
    Vol. 1. Embryonic stem cells -- Vol. 2. Adult and fetal stem cells.
    Also available: Print – v. 1-2, 2004.
  • 2010 Springer
    Motonari Kondo, editor.
  • 2006 Springer
    [edited by] Isabelle Godin, Ana Cumano.
    Origin and fate of hematopoietic precursors in the early mouse embryo / Julien Yuan Bertrand ... [et al.] -- Hematopoietic development in drosophila: a parallel with vertebrates / Marie Meister and Shubha Govind -- Intraembryonic development of hematopoietic stem cells during human ontogeny: expression analysis / Caroline Marshall -- Gene and cell therapy involving hematopoietic stem cell / Isabelle André-Schmutz and Marina Cavazzana-Calvo. Ventral and dorsal contributions to hematopoiesis in xenopus / Aldo Ciau-Uitz, Maggie Walmsley and Roger Patient -- Genetic dissection of hematopoiesis using the zebrafish / Rebecca A. Wingert and Leonard I. Zon -- Extra- and intraembryonic HSC commitment in the avian model / Thierry Jaffredo ... [et al.] -- Avian lymphopoiesis and transcriptional control of hematopoietic stem cell differentiation / Jussi Liippo and Olli Lassila -- Yolk sac development in mice / James Palis -- Long-term reconstituting hematopoietic stem cell capacity in the embryo / Mervin C. Yoder -- The endothelium: the cradle of definitive hematopoiesis? / Katrin Ottersbach and Elaine Dzierzak -- Endothelial and hematopoietic cells in the intraembryonic compartment / Fumio Arai and Toshio Suda --
    Also available: Print – 2006
  • 2014 Springer Protocols
    edited by Kevin D. Bunting and Cheng-Kui Qu, Department of Pediatrics, Aflac Cancer and Blood Disorders Center, Emory University School of Medicine, Atlanta, GA, USA.
  • 2009 Springer
    Michael R. Bishop, editor.
    Also available: Print – 2009
  • 2008 Springer
    edited by Robert J. Soiffer.
    Stem cell biology / Elizabeth O. Hexner and Stephen G. Emerson -- Stem cell transplantation for hematologic malignancies / Effie W. Petersdorf -- Immunobiology of transplantation / Robert S. Negrin -- Allogeneic and autologous hematopoietic stem cell transplantation for acute lymphoblastic leukemia and acute myelogenous leukemia in the adult / Anthony Stein and Stephen J. Forman -- Hematopoietic cell transplantation for chronic myeloid leukemia / Uday Popat and Sergio Giralt -- Stem cell transplantation for Hodgkin's and non-Hodgkin's lymphomas / John W. Sweetenham -- Transplantation in chronic lymphocytic leukemia / Rifca Le Diew and John G. Gribben -- Stem cell transplantation in multiple myeloma / John Koreth and Kenneth C. Anderson -- Allogeneic hematopoietic cell transplantation for patients with myelodysplastic syndrome and myeloproliferative disorders / Aravind Ramakrishnan and H. Joachim Deeg -- Stem cell transplantation for marrow failure / Philippe Armand and Joseph H. Antin -- Stem cell transplantation for hemoglobinopathies / Catherine J. Wu -- Stem cell sources for allogeneic transplantation / Corey Cutler -- Allogeneic unrelated donor blood and marrow transplantation / Daniel Weisdorf -- Umbilical cord hematopoietic stem cell transplantation / Mitchell E. Horwitz and Nelson Chao -- Haploidentical stem cell transplantation / Bimalangshu R. Dey and Thomas R. Spitzer -- Ablative preparative regimens for hematopoietic stem cell transplantation / Amin Alousi and Marcos de Lima -- Nonmyeloablative transplantation / Frédéric Baron and Brenda M. Sandmaier -- Immune reconstitution after allogeneic transplantation / Carolyn A. Keever-Taylor -- Infection in the hematopoietic stem cell transplant recipient / Francisco M. Marty and Lindsey R. Baden -- EBV lymphoproliferative disease after transplantation / Stephen Gottschalk, Cliona M. Rooney, and Helen E. Heslop -- Hepatic veno-occlusive disease / Erica Linden and Paul Richardson -- Lung injury after hematopoietic stem cell transplantation / Kenneth R. Cooke and Gregory Yanik -- Quality of life issues post-transplantation / Stephanie J. Lee -- Pathophysiology of acute graft-versus-host disease / Pavan Reddy and James L.M. Ferrara -- Acute graft-versus-host disease / Martin Benesch and H. Joachim Deeg -- Chronic Graft-versus-host disease / Javier Bolaños-Meade and Georgia B. Vogelsang -- Graft manipulation : T cell depletion and tumor cell purging / Vincent T. Ho -- Donor lymphocyte infusions : clinical applications and the graft-versus-leukemia effect / Edwin P. Alyea -- Regenerative medicine : application in cardiovascular diseases / Emerson C. Perin, Guilherme V. Silva, and Marlos R. Fernandes.
    Also available: Print – 2008
  • 2009 Springer Protocols
    edited by Anil Dhawan, Robin D. Hughes.
    Human hepatocyte transplantation overview / Juliana Puppi and Anil Dhawan -- Isolation of human hepatocytes / Ragai R. Mitry -- An optimised method for cryopreservation of human hepatocytes / Claire Terry and Robin D. Hughes -- Liver cell culture techniques / José V. Castell and María José Gómez-Lechón -- In-vitro assays for induction of drug metabolism / Brian G. Lake ... [et al.] -- Hepatocyte apoptosis / Mustapha Najimi, Françoise Smets, and Etienne Sokal -- Small animal models of hepatocyte transplantation / Jurgen Seppen, Ebtisam El Filali, and Ronald Oude Elferink -- Hepatocyte transplantation techniques : large animal models / Anne Weber, Marie Thérèse Groyer-Picard, and Ibrahim Dagher -- Cell transplant techniques : engraftment detection of cells / Robert A. Fisher and Valeria R. Mas -- Hepatic preconditioning for transplanted cell engraftment and proliferation / Yao-Ming Wu and Sanjeev Gupta -- Ex vivo gene transfer into hepatocytes / Xia Wang ... [et al.] -- Sources of adult hepatic stem cells : haematopoietic / Rosemary Jeffery, Richard Poulsom, and Malcolm R. Alison -- Production of hepatocyte-like cells from human amnion / Toshio Miki ... [et al.] -- Generation of hepatocytes from human embryonic stem cells / Niloufar Safinia and Stephen L. Minger -- Isolation, in vitro cultivation, and characterisation of foetal liver cells / Yue Wu, Chetan C. Shatapathy, and Stephen L. Minger -- Human intrahepatic biliary epithelial cell lineages : studies in vitro / Ruth Joplin and Stivelia Kachilele -- Liver cell labelling with MRI contrast agents / Michel Modo, Thomas J. Meade, and Ragai R. Mitry -- Microbiological monitoring of hepatocyte isolation in the GMP laboratory / Sharon C. Lehec.
  • 2010 Springer Protocols
    edited by Patrick Maurel.
    1. General review on in vitro hepatocyte models and their applications / Christiane Guguen-Guillouzo and Andre Guillouzo -- 2. Human foetal hepatocytes / Anne Weber ... [et al.] -- 3. Isolation and culture of primary hepatocytes from resected human liver tissue / Edward L. LeCluyse and Eliane Alexandre -- 4. Optimisation of the cryopreservation of primary hepatocytes / Nicola J. Hewitt -- 5. Cryopreservation of human hepatocytes for clinical use / Ragai R. Mitry, Sharon C. Lehec, and Robin D. Hughes -- 6. Hepatocyte differentiation / Katy M. Olsavsky Goyak, Elizabeth M. Laurenzana, and Curtis J. Omiecinski -- 7. Reversible manipulation of apoptosis sensitivity in cultured hepatocytes by matrix-mediated manipulation of signaling activities / Patricio Godoy ... [et al.] -- 8. Markers and signaling factors for stem cell differentiation to hepatocytes / Frédéric Lemaigre -- 9. Hepatic stem cells / Robert E. Schwartz and Catherine Verfaillie -- 10. Hepatic stem cells and liver development / Nalu Navarro-Alvarez, Alejandro Soto-Gutierrez, and Naoya Kobayashi -- 11. Generation of hepatocytes from human embryonic stem cells / Neta Lavon -- 12. Isolation and culture of adult human liver progenitor cells / Sabine Gerbal-Chaloin ... [et al.] -- 13. The HepaRG cell line / Marie-Jeanne Marion, Olivier Hantz, and David Durantel -- 14. Transdifferentiation of pancreatic cells to hepatocytes / Chia-Ning Shen and David Tosh -- 15. Evaluation of drug metabolism, drug-drug interactions and in vitro hepatotoxicity with cryopreserved human hepatocytes / Albert P. Li -- 16. The use of human hepatocytes to investigate drug metabolism and CYP enzyme induction / Sylvie Klieber ... [et al.] -- 17. The use of hepatocytes to investigate UDP-glucuronosyltransferases and sulfotransferases / Sylvie Fournel-Gigleux ... [et al.] -- 18. The use of hepatocytes to investigate drug uptake transporters / Kazuya Maeda and Yuichi Sugiyama -- 19. Metabonomic studies on human hepatocyte in primary culture / Vincent Croizmarie ... [et al.] -- 20. The application of HepRG cells in evaluation of cytochrome P450 induction properties of drug compounds / Tommy B. Andersson -- 21. The use of hepatocytes to investigate drug toxicity / María José Gómez-Lechón, José V. Castell, Maria Teresa Donato -- 22. The use of human hepatocytes to investigate bile acid synthesis / Ewa C.S. Ellis and Lisa-Mari Nilsson -- 23. The use of human hepatocytes to investigate blood coagulation factor / Christine Biron-Andréani ... [et al.] -- 24. Use of human hepatocytes to investigate HCV infection / Lydiane Pichard-Garcia ... [et al.] -- 25. The use of hepatocytes to investigate HDV infection / Camille Sureau -- 26. Rodent models of liver repopulation / Helène Gilgenkrantz -- 27. Chimeric mice with humanized liver / Stephen C. Strom, Julio Davila, and Markus Grompe -- 28. Bioartificial liver support systems / Gesine Pless -- 29. Human hepatocyte transplantation / Anil Dhawan ... [et al.].
  • 2006 Springer Protocols
    edited by D. Lansing Taylor, Jeffrey R. Haskins, Kenneth A. Giuliano.
  • 2014 Springer
    Harris G. Fienberg, Garry P. Nolan, editors.
    This volume highlights the most interesting biomedical and clinical applications of high-dimensional flow and mass cytometry. It reviews current practical approaches used to perform high-dimensional experiments and addresses key bioinformatic techniques for the analysis of data sets involving dozens of parameters in millions of single cells. Topics include single cell cancer biology; studies of the human immunome; exploration of immunological cell types such as CD8+ T cells; decipherment of signaling processes of cancer; mass-tag cellular barcoding; analysis of protein interactions by proximity ligation assays; Cytobank, a platform for the analysis of cytometry data; computational analysis of high-dimensional flow cytometric data; computational deconvolution approaches for the description of intracellular signaling dynamics; and hyperspectral cytometry. All 10 chapters of this book have been written by respected experts in their fields. It is an invaluable reference book for both basic and clinical researchers.
    Also available: Print – 2014
  • 2007
    Ronald W. Dudek.
    Status: Not Checked OutLane Catalog Record
  • 2013 Springer
    Moshe Oren, Yael Aylon, editors.
    The Hippo signaling pathway is rapidly gaining recognition as an important player in organ size control and tumorgenesis. This volume presents virtually all aspects of tumor biology because members of the Hippo Pathway have been associated with numerous well-established cell signaling pathways, just to name a few; Rho, Wnt, TGFbeta and p53. Hippo signaling is not solely involved in regulating "classic" tumor characteristics such as cell proliferation, survival and growth, but is also diversely involved in cell-autonomous and non-cell-autonomous differentiation, migration and organ size control. With the multitude of signaling events mediated by the Hippo pathway and the vastly different functions that it plays, it is evident that these tumor suppressors are unique governors of cellular homeostasis. This timely volume gathers wide-ranging and burgeoning information on the Hippo pathway and its role in cancer into an accessible format of a single book.With the multitude of signaling events mediated by the Hippo pathway and the vastly different functions that it plays, it is evident that these tumor suppressors are unique governors of cellular homeostasis. This timely volume gathers wide-ranging and burgeoning information on the Hippo pathway and its role in cancer into an accessible format of a single book.
  • 2013 CRCnetBASE
    edited by Jean-Marie Exbrayat.
    Section 1. Histological and histochemical methods with light microscopy -- section 2. Cytological methods in electron microscopy -- section 3. Image quantification.
  • 2010 AccessMedicine
    Douglas F. Paulsen.
    Pt 1. Fundamental concepts -- pt 2. The four basic tissue types -- pt 3. Organs and organ systems .
  • 2016 ClinicalKey
    Abraham L. Kierszenbaum, Laura L. Tres.
    Epithelium -- Epithelial glands -- Cell signaling -- Connective tissue -- Osteogenesis -- Blood and hematopoiesis -- Muscle tissue -- Nervous tissue -- Sensory organs vision and hearing -- Immune-lymphatic system -- Integumentary system -- Cardiovascular system -- Respiratory system -- Urinary system -- Upper digestive system -- Lower digestive system -- Digestive glands -- Neuroendocrine system -- Endocrine system -- Spermatogenesis -- Sperm transport and maturation -- Follicle development and the menstrual cycle -- Fertilization, placentation, and lactation.
  • 2013 Springer
    Li Wu, Olivier Schwartz, editors.
    Also available: Print – 2013
  • 2009 Springer
    Paul Spearman, Eric O. Freed, editors.
    Preface -- Host restriction of HIV-1 by APOBEC3 and viral evasion through Vif -- Interactions of viral protein U (Vpu) with cellular factors -- TRIM5alpha -- Human immunodeficiency virus type-1 Gag and host vesicular trafficking pathways -- The roles of tetraspanins in HIV-1 replication -- Imaging of HIV/host protein interactions -- Virological and cellular roles of the transcriptional coactivator LEDGF/p75 -- Implications of Nef: host cell interactions in viral persistence and progression to AIDS -- Vpr and its interactions with cellular proteins.
    Also available: Print – 2009
  • 2014 Springer
    Gaspar Banfalvi.
    Human homeostasis refers to complex interactions to regulate and maintain the internal conditions of the organism under stable condition. In spite of the progress made on cell growth and division, the circuits that coordinate these processes and maintain the homeostatic balance between cell growth and cell death have not been clarified. Deregulation of homeostatic processes results in different forms of imbalances that can turn to diseases including hepatitis, cyrrhosis and tumor formation. Abnormal growth of tissues can lead to a broad spectrum of neoplastic diseases from tissue swelling, primary tumor formation, cancer and ultimately to secondary tumors known as metastases. Tumor formation is a complex process, that develops through many steps. The discussion of balance and its loss in cell growth, primary and secondary tumor formation necessitate basic information in chemistry (carcinogenesis), biochemistry (metabolism), physics (instrumentation), cell biology (compartments), anatomy, physiology, clinical experience (screening, medical imaging, therapy). The focus of this book has been placed on: a) balance of cell growth, b) tumor formation, c) development of cancer and metastasis, e) diagnosis and treatment of cancer. The steps of the lymphatic type of metastatic tumor spread are described. Due to the interdisciplinary nature, the book gives information for a wide audience including biology, biotechnology, molecular, ecology, medical, pharmacist, chemist students. Additional information is provided for biomedical, pharma, medical and life scientists, physicians and health care professionals.
  • 2008 Springer
    Shlomo Melmed ... [et al.], eds.
  • 2009 Springer
    edited by John R. Masters and Bernhard Ø. Palsson.
    1. Neural progenitors / Dustin R. Wakeman ... [et al.] -- 2. Multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.] -- 3. Endothelium / Sangmo Kwon, Takayuki Asahara -- 4. Lung / Rabindra Tirouvanziam, Megha Makam, Bruno Péault -- 5. Eye / Maria Notara ... [et al.] -- 6. Colon / F. Iovino ... [et al.] -- 7. Spermatogonia / Makoto C. Nagano, Jonathan R. Yeh, Khaled Zohni -- 8. Hair follicle pluripotent stem (hfPS) cells / Robert M. Hoffman -- 9. Pancreas / Fang-Xu Jiang, Grant Morahan -- 10. Prostate / C. Foley ... [et al.].
  • 2012 Springer Protocols
    edited by Ragai R. Mitry, Robin D. Hughes.
    1. Introduction to cell culture / Christina Philippeos, Robin D. Hughes, Anil Dhawan and Ragai R. Mitry -- 2. Isolation and cultivation of dermal stem cells that differentiate into functional epidermal melanocytes / Ling Li, Mizubo Fukunaaga -- 3. Isolation, cultivation, and application of human alveolar epithelial cells / Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, Hanno Huwer, and Claus-Michael Lehr -- 4. Culture of parathyroid cells / Peyman Björklund and Per Hellman -- 5. Functional analysis of human islets of langerhans maintained in culture / Shanta J. Persaud, Bo Liu, and Peter M. Jones -- 6. Conversion of non-endocrine human pancreatic cells to insulin-producing cells for treatment of diabetes / Min Zhao and Guo Cai Huang -- 7. Evaluation of cytochrome P450 activities in human hepatocytes in vitro / María José Gómez-Lechón, Agustín Lahoz, José V. Castell, and María Teresa Donato -- 8. Human hepatocytes: isolation, culture, and quality procedures / Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, Peter Büchler, Michael Schoenberg, Jörg Kleeff, Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, Jan Hengstler, and Andrea K. Nussler -- 9. Preclinical testing ov virotherapeutics for primary and secondary tumors of the liver / Martina Zimmermann, Timo Weiland, Michael Bitzer, and Ulrich M. Lauer -- 10. Isolation and functional studies of human fetal gastric epithelium in primary culture / Pierre Chailler, Jean-Franc̦ois Beaulieu, and Daniel Ménard -- 11. Isolation, characterization, and culture of normal human intestinal crypt and villus cells / Jean-Franc̦ois Beaulieu and Daniel Ḿenard -- 12. Primary culture of human renal proximal tubule epithelial cells and interstitial fibroblasts / Claire C. Sharpe and Mark E.C. Dockrell -- 13. Glomerular epithelial and mesangial cell culture and characterization / Heather M. Wilson and Keith N. Stewart -- 14. Culture of isolated human adipocytes and isolated adipose tissue / Kirstin A. Carwswell, Mi-Jeong Lee, and Susan K. Fried -- 15. Primary culture of human adipocyte precursor cells: expansion and differentiation / Thomas Skurk and Hans Hauner -- 16. Primary culture of ovarian cells for research on cell interactions in the hormonal control of steroidogenesis / Jerzy F. Galas -- 17. Human vascular smooth muscle cell culture / Diane Proudfoot and Catherine Shanahan -- 18. Culture of human endothelial cells from umbilical veins / Richard C.M. Siow -- 19. Human peripheral blood mononuclear cell culture for flow cytometric analysis of phosphorylated mitogen-activated protein kinases / Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, and Dimitrios P. Bogdanos -- 20. Human CD4+CD25(high [superscript]) CD127 (low/neg [superscript]) regulatory T cells / Haibin Su, Maria Serena Longhi, Pengyun Wang, Diego Vergani, and Yun Ma -- 21. Human chondrocyte cultures as models of cartilage-specific gene regulation / Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring -- 22. Isolation and culture of human osteoblasts / Alison Gartland, Robin M.H. Rumney, Jane P. Dillon, and James A. Gallagher -- 23. Human osteoclast culture and phenotypic characterization / Ankita Agrawal, James A. Gallagher, and Alison Gartland -- 24. Effects of temperature generated from the holmium: Yag laser on human osteoblasts in monolayer tissue culture / Moustafa I. Hafez, Anne Sandison, Richard R.H. Coombs, Ian D. McCarthy, and Al-Shymaa M. Hafez -- 25. Laser microdissection microscopy: application to cell culture / Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia -- 26. Automated adherent human cell culture (mesenchymal stem cells) / Robert Thomas and Elizabeth Ratcliffe -- 27. Culturing and differentiating human mesenchymal stem cells for biocompatible scaffolds in regenerative medicine / William R. Otto and Catherine E. Sarraf.
  • 2011 Springer
    Johng S. Rhim, Richard Kremer, editors.
    Pt. 1. Multistep models -- pt. 2. Prostate cancer/stem cells -- pt. 3. Breast cancer/stem cells -- pt. 4. Multistep/genes.
    Also available: Print – 2011
  • 2010 Springer Protocols
    edited by Kursad Turksen.
    Human embryo culture and assessment for the derivation of embryonic stem cells (ESC) / A. Henry Sathananthan and Tiki Osianlis -- Derivation of human embryonic stem cell lines from vitrified human embryos / Teija T. Peura, Julia Schaft, and Tomas Stojanov -- Growth of human embryonic stem cells using derivates of human fibroblasts / Carmen Escobedo-Lucea and miodrag Stojkovic -- In vitro neural differentiation of human embronic stem cells using a low-density mouse embryonic fibroblast feeder protocol / John A. Ozolek [and others] -- Optimization of physiological xenofree molecularly defined media and matrices to maintain human embryonic stem cell pluripotency / Isabelle Pfeiffer [and others] -- Serum-free and feeder-free culture expansion of human embryonic stem cells / Katherine E. Wagner and Mohan C. Vemuri -- Single cell enzymatic dissociation of human embryonic stem cells : a straightforward, robust, and standardized culture method / Catharina Ellerström, Johan Hyllner, and Raimund Strehl -- Monitoring stemness in long-term hESC cultures by real-time PCR / Amparo Galán and Carlos Simón -- Culture and preparation of human embryonic stem cells for proteomics-based applications / Charles C. King -- A two- and three-dimensional approach for visualizing human embryonic stem cell differentiation / Christian B. Brøchner [and others] -- Immunofluorescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions / Aashir Awan [and others] -- Study of gap junctions in human embryonic stem cells / Raymond C.B. Wong and Alice Pébay -- hESC engineering by integrase-mediated chromosomal targeting / Ying Liu [and others] -- Human embryonic stem cell differentiation on periodontal ligament fibroblasts / Y. Murat Elc̦in, Bülend İnanc̦, and A. Eser Elc̦in -- Generation of neural crest cells and peripheral sensory neurons from human embryonic stem cells / Ronald S. Goldstein [and others] -- Embryonic stem cells as a model for studying melanocyte development / Susan E. Zabierowski and Meenhard Herlyn -- In vitro derivation of chondrogenic cells from human embryonic stem cells / Wei Seong Toh [and others] -- Vascular differentiation of human embryonic stem cells in bioactive hydrogel-based scaffolds / Sharon Gerecht, Lino S. Ferreira, and Robert Langer -- Differentiation of neural precursors and dopaminergic neurons from human embryonic stem cells / Xiao-Quing Zhang and Su-Chung Zhang -- Transplantation of human embryonic stem cells and derivatives to the chick embryo / Ronald S. Goldstein -- Genetic manipulation of human embryonic stem cells / Silvina Epsztejn-Litman and Rachel Eiges -- Genetic manipulation of human embryonic stem cells in serum and feeder-free media / Stefan R. Braam, Chris Denning, and Christine L. Mummery -- Human-induced pluripotent stem cells : derivation, propagation, and freezing in serum- and feeder layer-free culture conditions / Hossein Baharvand [and others].
  • 2015 Springer Protocols
    edited by Kursad Turksen.
    Derivation of human embryonic stem cell lines from vitrified human blastocysts / Cara K. Bradley ... [et al.] - Characterizing pluripotent stem sells using the TaqMan® hPSC scorecardTM panel / Jeffrey Fergus, Rene Quintanilla, and Uma Lakshmipathy -- Growth of human pluripotent stem cells using functional human extracellular matrix / Andres Sanz-Garcia, Miodrag Stojkovic, and Carmen Escobedo-Lucea -- Efficient expansion of dissociated human pluripotent stem cells using a synthetic substrate / Eihachiro Kawase -- Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells / Noboru Sato and Ali Brivanlou -- Monitoring stemness in long-term hESC sultures by real-time PCR / Amparo Galan and Carlos Simon -- Study of gap junctions in human embryonic stem cells / Alice Pebay and Raymond C.B. Wong -- Immunofluorescence microscopy and mRNA analysis of human embryonic stem cells (hESCs) including primary cilia associated signaling pathways / Maj Linea Vestergaard ... [et al.] -- Analysis of intracellular calcium signaling in human embryonic stem cells / Adrienn Pentek, Katalin Paszty, and Agota Apati -- Genetic manipulation of human embryonic stem cells / Rachel Eiges -- Genetic modification in human pluripotent stem cells by homologous recombination and CRISPR/Cas9 system / Haipeng Xue ... [et al.] -- Use of multicolor flow cytometry for isolation of specific cell populations deriving from differentiated human embryonic stem cells / Isabella Mengarelli, Andrew Fryga, and Tiziano Barberi -- Definitive endoderm differentiation of human embryonic stem cells combined with selective elimination of undifferentiated cells by methionine deprivation / Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume -- Derivation of endothelial cells and pericytes from human pluripotent stem cells / Sravanti Kusuma and Sharon Gerecht -- Differentiation of human embryonic stem cells on periodontal ligament fibroblasts / Y. Murat Elcin, Bulend Inanc, and A. Eser Elcin -- Derivation of epithelial cells from human embryonic stem cells as an in vitro model of vocal mucosa / Vlasta Lungova, Ciara Leydon, and Susan Thibeault -- Human embryonic and hepatic stem cell differentiation visualized in two and three dimensions based on serial sections / Peter S. Vestentoft ... [et al.] -- Derivation of chondrogenic cells from human embryonic stem cells for cartilage tissue engineering / Wei Seong Toh and Tong Cao -- Microgrooved surface modulates neuron differentiation in human embryonic stem cells / David Lu ... [et al.] -- Directed differentiation of human embryonic stem cells into neural progenitors / Erin Banda and Laura Grabel -- Direct conversion of pluripotent human embryonic stem cells under defined culture conditions into human neuronal or cardiomyocyte cell therapy derivatives / Xuejun H. Parsons -- Generation of epithelial cell populations from human pluripotent stem cells using a small-molecule inhibitor of Src family kinases / Joshua A. Selekman, Xiaojun Lian, and Sean P. Palecek -- Dual-SMAD inhibition/WNT activation-based methods to induce neural crest and derivatives from human pluripotent stem cells / Stuart M. Chambers ... [et al.] -- Accelerated three-dimensional neuroepithelium formation from human embryonic stem cells and its use for quantitative differentiation to human retinal pigment epithelium / Yu Zhu, Sven Schreiter, and Elly M. Tanaka -- Efficient production of photoreceptor precursor cells from human embryonic stem cells / Anat Yanai ... [et al.] -- Generation of megakaryocytes and platelets from human pluripotent stem cells / Marjorie Pick -- Simple protocol for the generation of cardiomyocytes from human pluripotent stem cells / Glen Lester Sequiera, Ashish Mehta, and Winston Shim -- Erratum to: Microgrooved surface modulates neuron differentiation in human embryonic stem cells / David Lu ... [et al.].
  • 2006 Springer Protocols
    edited by Kursad Turksen.
    Human embryonic stem cells: isolation, maintenance and differentiation / Kursad Turksen and Tammy-Claire Troy -- Blastocyst culture for deriving human embryonic stem cells / Ariff Bongso -- Propagation of human embryonic stem cells on human feeder cells / Mark Richards and Ariff Bongso -- Derivation and maintenance of human embryonic stem cells / Michal Amit and Joseph Itskovitz-Eldor -- Isolation and characterization of human embryonic stem cells / Maisam Mitalipova and Gail Palmarini -- Routine culture and differentiation of human embryonic stem cells / Jim McWhir, Davina Wojtacha, and Alison Thomson -- Culture of human embryonic stem cells on human and mouse feeder cells / Gautam Dravid, Holly Hammond, and Linzhao Cheng -- Maintenance of human embryonic stem cells in animal serum- and feeder layer- free culture conditions / Michal Amit and Joseph Itskovitz-Eldor -- Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor / Noboru Sato and Ali H. Brivanlou -- Derivation of human feeders for prolonged support of human embryonic stem cells / Chui-Yee Fong and Ariff Bongso -- Transplantation of human embryonic stem cells to the chick embryo / Ronald S. Goldstein -- Derivation and characterization of neuronal precursors and dopaminergic neurons from human embryonic stem cells in vitro / Melissa Carpenter ... [et al.] -- In vitro differentiation of neural precursors from human embryonic stem cells / Xue-Jun Li and Su-Chun Zhang -- Derivation and characterization of hematopoietic cells from human embryonic stem cells / Lisheng Wang ... [et al.] -- Retroviral transduction of hematopoietic progenitors derived from human embryonic stem cells / Pablo Menendez ... [et al.] -- Genetic manipulation of human embryonic stem cells by transfection / Rachel Eiges -- Designing, testing, and validating a microarray for stem cell characterization / Yongquan Luo ... [et al.] -- Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells / Noboru Sato and Ali H. Brivanlou -- Massively parallel signature sequencing / Daixing Zhou ... [et al.] -- Confrontation cultures of embryonic stem cells with multicellular tumor spheroids to study tumor-induced angiogenesis / Maria Wartenberg ... [et al.] -- A novel experimental platform for investigating cancer growth and anti-cancer therapy in a human tissue microenvironment derived from human embryonic stem cells / Maty Tzukerman and Karl L. Skorecki -- The analysis of mitochondria and mitochondrial DNA in human embryonic stem cells / Justin C. St. John ... [et al.].
  • 2012 Springer Protocols
    edited by Kursad Turksen.
    1. Establishment of new lines of human embryonic stem cells: evolution of the methodology / Ana Maria Fraga, Érica Sara Souza de Araújo, Raquel Stabellini, Naja Vergani, and Lygia V. Pereira -- 2. Human embryonic stem cells derived in xeno-free conditions / Amparo Galán and Carlos Simón -- 3. Procedures for derivation and characterisation of human embryonic stem cells from Odense, Denmark / Linda Harkness and Moustapha Kassem -- 4. Principles for derivation of human embryonic stem cells / Mikael C.O. Englund, Catharina Ellerström, Katarina Andersson, Karin Noaksson, and Johan Hyllner -- 5. Derivation and maintenance of undifferentiated human embryonic stem cells / Guoliang Meng and Derrick E. Rancourt -- 6. Establishment of hESC lines from the inner cell mass of blastocyst-stage embryos and single blastomeres of 4-cell stage embryos / Ileana Mateizel, Mieke Geens, Hidle Van de Velde, and Karen Sermon -- 7. Analysis of LINE-1 expression in human pluripotent cells / Martin Muñoz-Lopez, Marta Garcia-Cañadas, Angela Macia, Santiago Morell, and Jose L. Garcia-Perez -- 8. Characterization and gene expression profiling of five human embryonic stem cell lines derived in Taiwan / Steven Shoe-Lung Li -- 9. Derivation of human embryonic stem cell lines from poor quality embryos / Ronak Shetty and Maneesha S. Inamdar -- 10. Derivation, expansion, and characterization of human embryonic stem cell lines from aneuploid embryos / Juan-Carolos Biancotti and Neta Lavon -- 11. Mutated human embryonic stem cells for the study of human genetic disorders / Ahmi Ben-Yehudah, Mira Malcov, Tsvia Frumkin, and Dalit Ben-Yosef -- 12. Single-cell enzymatic dissociations of hESC lines OxF1-OxF4 and culture in feeder-free conditions / Frances A. Brook -- 13. Protocol for expansion of undifferentiated human embryonic and pluripotent stem cells in suspension / Hossein Baharvand, Mehran Rezaei Larijani, and Maryam Yousfi -- 14. Suspension bioreactor expansion of undifferentiated human embryonic stem cells / Roman Krawetz and Derrick E. Rancourt -- 15. Derivation, propagation, and characterization of neuroprogenitors from pluripotent stem cells (hESCs and hiPSCs) / Khun-Hong Lie, Henry C.Y. Chung, and Kuldip S. Sidhu -- 16. Comparison of neural differentiation potential of human pluripotent stem cell lines using a quantitative neural differentiation protocol / Dezhong Yin, Tahereh Tavakoli, Wei-Qiang Gao, and Wu Ma --17. Array-comparative genomic hybridization characterization of human pluripotent stem cells / Aaron M. Elliott, Kristi A. Hohenstein Elliott, and Anja Kammesheidt -- 18. Chromatin immunoprecipitation-based analysis of gene regulatory networks operative in human embryonic stem cells / Marc Jung and James Adjaye -- 19. Analysis of the methylome of human embryonic stem cells employing methylated DNA immunoprecipitation coupled to next generation sequencing / Christina Grimm and James Adjaye -- 20. Stable isotope labelling with amino acids in cell culture for human embryonic stem cell proteomic analysis / Linda Harkness, Tatyana A. Prokhorova, Moustapha Kassem, and Blagoy Blagoev.
  • 2014 Springer Protocols
    edited by Zev Rosenwaks, Paul M. Wassarman.
    General aspects of fertility and infertility / Mark A. Damario -- Genetics of male fertility / Yi-Nan Lin and Martin M. Matzuk -- Genetics of female infertility due to anomalies of the ovary and mullerian ducts / Joe Leigh Simpson -- Gene polymorphisms in female reproduction / Livio Casarini and Manuela Simoni -- Understanding the spermatozoon / Queenie V. Neri ... [et al.] -- Derivation of human embryonic stem cells (hESC) / Nikica Zaninovic, Qiansheng Zhan, and Zev Rosenwaks -- Endocrinology of the menstrual cycle / Robert L. Barbieri -- Assisted reproductive techniques / Jack Yu Jen Huang and Zev Rosenwaks -- Novel markers of male infertility / Michael Funaro and Darius A. Paduch -- Luteal phase support in ART treatments / Yuval Or, Edi Vaisbuch, and Zeev Shoham -- General principles of cryopreservation / Roger G. Gosden -- In vitro maturation of immature human oocytes for clinical application / Ri-Cheng Chian and Yun-Xia Cao -- GnRH antagonist-based protocols for in vitro fertilization / David Reichman and Zev Rosenwaks -- Ovarian stimulation for IVF : mild approaches / O. Hamdine, F. J. Broekmans, and B. C. J. M. Fauser -- IVF stimulation : protocols for poor responders / Owen K. Davis -- Oocyte retrieval and quality evaluation / Lucinda Veeck Gosden -- Sperm retrieval and quality evaluation / Peter J. Stahl, Peter N. Schlegel, and Marc Goldstein -- Treatment of male infertility / Gianpiero D. Palermo, Justin Kocent, Devin Monahan, Queenie V. Neri, and Zev Rosenwaks -- Techniques for slow cryopreservation of embryos / Lucinda Veeck Gosden -- Cryopreservation of eggs / Zsolt Peter Nagy ... [et al.] -- Ovarian tissue cryopreservation and transplantation : a realistic, effective technology for fertility preservation / Dror Meirow, Hila Raanani, and Hannah Biderman -- Detection of monogenic disorders and chromosome aberrations by preimplantation genetic diagnosis / Kangpu Xu and David Reichman -- Embryo culture and selection : morphological criteria / Aparna Hegde and Barry Behr -- Embryo selection using metabolomics / D. Sakkas -- Embryo transfer / Anate Aelion Brauer and Glenn Schattman -- Safety of intracytoplasmic sperm injection / Gianpiero D. Palermo, Queenie V. Neri, and Zev Rosenwaks -- Human germ cell differentiation from pluripotent embryonic stem cells and induced pluripotent stem cells / Jose V. Medrano, Carlos Simon, and Renee Reijo Pera.
  • 2013 Springer
    Yuri B. Yurov, Svetlana G. Vorsanova, Ivan Y. Iourov, editors.
  • 2011 Springer Protocols
    edited by Philip H. Schwartz, Robin L. Wesselschmidt.
    The Stem Cell Laboratory: Design, Equipment, and Oversight -- Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research -- Embryonic Stem Cell Derivation from Human Embryos -- Derivation of Human Parthenogenetic Stem Cell Lines -- Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfer -- Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction -- Transgene-free Production of Pluripotent Stem Cells using PiggyBac Transposons -- Traditional Human Embryonic Stem Cell Culture -- Xeno-Free Culture of Human Pluripotent Stem Cells -- Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically-Defined Medium with Enzymatic Single-Cell Passaging -- GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications -- Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging -- Classical Cytogenetics: Karyotyping Techniques -- FISH Analysis of Human Pluripotent Stem Cells -- Immunocytochemical Analysis of Human Pluripotent Stem Cells -- Flow Cytometric Analysis of Human Pluripotent Stem Cells -- The Teratoma Assay: An In vivo Assessment of Pluripotency -- Detection of Copy Number Variation using SNP Genotyping -- Genome-wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq -- Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays -- Development of High Content Screening Approaches and Analysis for Human Pluripotent Stem Cells -- Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells -- Lentivirus-mediated Modification of Pluripotent Stem Cells -- Nucleofection of Human Embryonic Stem Cells -- Non-viral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells -- Gene Targeting in Human Pluripotent Stem Cells -- Episomal Transgene Expression in Pluripotent Stem Cells -- The Generation of Embryoid Bodies from Feeder-based or Feeder-free Human Pluripotent Stem Cell Cultures -- Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells -- Directed Differentiation of Dopamine Neurons From Human Pluripotent Stem Cells -- Methods for the Derivation and Use of Cardiomyocytes from Human Pluripotent Stem Cells -- In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells -- Differentiation of Dendritic Cells from Human Embryonic Stem Cells.
  • 2009 ScienceDirect
    edited by Robert Stern.
    Association between cancer and "acid mucopolysaccharides" : an old concept comes of age, finally -- Hyaluronan-CD44 interactions and chemoresistance in cancer cells -- Growth factor regulation of hyaluronan deposition in malignancies -- Hyaluronan binding protein 1 (HABP1/p32/gClqR) : a new perspective in tumor development -- CD44 meets Merlin and Ezrin : their interplay mediates the pro-tumor activity of CD44 and tumor-suppressing effect of Merlin -- Hyaluronan-mediated CD44 interaction with receptor and non-receptor kinases promotes oncogenic signaling, cytoskeleton activation, and tumor progression -- Adhesion and penetration : two sides of CD44 signal transduction cascades in the context of cancer cell metastasis -- Involvement of CD44, a molecule with a thousand faces, in cancer dissemination -- RHAMM/HMMR : an itinerant and multifunctional hyaluronan binding protein that modifies CD44 signaling and mitotic spindle formation -- Altered hyaluronan biosynthesis in cancer progression -- Hyaluronidase : both a tumor promoter and suppressor -- The hyaluronidases in cancer biology -- Hyaluronan fragments : informational polymers commandeered by cancers -- Hyaluronan in human tumors : importance of stromal and cancer cell-associated hyaluronan -- The oncofetal paradigm revisited : MSF and HA as contextual drivers of cancer progression -- Hyaluronan synthesis and turnover in prostate cancer -- Role of hyaluronan in melanoma progression -- Role of hyaluronan metabolism in the initiation, invasion, and metastasis of breast cancer -- Clinical use of hyaluronidase in combination cancer chemotherapy : a historic perspective -- Exploring the hyaluronan:CD44 interaction for cancer therapy -- Hyaluronidase-2 and its role as a cell-entry receptor for sheep retroviruses that cause contagious respiratory tract cancers.
  • pt. A-C=, 2013 ScienceDirect
    pt. B ScienceDirect
    pt. C ScienceDirect
    volume editor, Lester Packer, Enrique Cadenas.
  • 2009 Springer
    Sara Gil-Perotín, Arturo Álvarez-Buylla and José Manuel Garcia-Verdugo.
    Adult neurogenesis has been questioned for many years. In the early 1900s, a dogma was established that denied new neuron formation in the adult brain. Adult neural stem cells are undifferentiated cells that are present in the adult brain and are capable of dividing and differentiating into glia and new neurons.
  • 2013 Springer
    Valérie Quesniaux, Bernhard Ryffel, Franco Di Padova, editors.
    The knowledge of Th17 cells and other cell populations which secrete IL-17A, and/or IL-22 has expanded tremendously since the publication of the first edition "Th17 Cells: Role in Inflammation and Autoimmune Disease" in 2008. The present volume has been completely revised with the addition of new chapters on the IL-17 receptor family and signaling, and an in-depth review of IL-22 and innate lymphoid cells. The differentiation of naïve T cells into regulatory T cells and Th17 cells as well as the plasticity of Th17 cells is discussed.
    Also available: Print – 2013
  • 2013 Springer Protocols
    edited by Kursad Turksen.
    Primary Culture and Live Imaging of Adult Neural Stem Cells and Their Progeny / Felipe Ortega, Benedikt Berninger, and Marcos R. Costa -- Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology / Marie-Therese Armentero, Patrizia Bossolasco, and Lidia Cova -- High Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes / Gábor Földes and Maxime Mioulane -- A High Content Imaging-Based Approach for Classifying Cellular Phenotypes / Joseph J. Kim, Sebastián L. Vega, and Prabhas V. Moghe -- Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture Conditions / Go Nagamatsu and Toshio Suda -- Imaging and Tracking of Bone-Marrow-Derived Immune and Stem Cells / Youbo Zhao [and four others] -- Co-Visualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3-D DNA Methylation Phenotyping of Stem Cells / Jian Tajbakhsh -- Noninvasive Imaging of Myocardial Blood Flow Recovery in Response to Stem Cell Intervention / HuaLei Zhang and Rong Zhou -- Live Imaging of Early Mouse Embryos Using Fluorescently Labeled Transgenic Mice / Takaya Abe, Shinichi Aizawa, and Toshihiko Fujimori -- Live Imaging, Identifying, and Tracking Single Cells in Complex Populations In Vivo and Ex Vivo / Minjung Kang [and four others] -- Quantitative Evaluation of Stem Cell Grafting in the Central Nervous System of Mice by In Vivo Bioluminescence Imaging and Postmortem Multicolor Histological Analysis / Kristien Reekmans [and eight others] -- Micro-CT Technique for Three Dimensional Visualization of Human Stem Cells / Andrea Farini [and four others] -- Noninvasive Multimodal Imaging of Stem Cell Transplants in the Brain Using Bioluminescence Imaging and Magnetic Resonance Imaging / Annette Tennstaedt [and three others] -- Magnetic Resonance Imaging and Tracking of Stem Cells / Hossein Nejadnik, Rostislav Castillo, and Heike E. Daldrup-Link -- Whole Body MRI and Fluorescent Microscopy for Detection of Stem Cells Labeled with Superparamagnetic Iron Oxide (SPIO) Nanoparticles and DiI Following Intramuscular and Systemic Delivery / Boris Odintsov, Ju Lan Chun, and Suzanne E. Berry -- Molecular Imaging and Tracking Stem Cells in Neurosciences / Toma Spiriev, Nora Sandu, and Bernhard Schaller -- Bioluminescence Imaging of Human Embryonic Stem Cells-Derived Endothelial Cells for Treatment of Myocardial Infarction / Weijun Su [and four others].
  • 2013 Springer Protocols
    edited by Jose M. Guisan.
    New opportunities for immobilization of enzymes / Jose M. Guisan -- Immobilization of enzymes : a literature survey / Beatriz Brena, Paula González-Pombo, and Francisco Batista-Viera -- Glutaraldehyde-mediated protein immobilization / Fernando López-Gallego, Jose M. Guisán, and Lorena Betancor -- Immobilization of enzymes on monofunctional and heterofunctional epoxy-activated supports / Cesar Mateo, Valeria Grazu, and Jose M. Guisan -- Stabilization of enzymes by multipoint covalent immobilization on supports activated with glyoxyl groups / Fernando López-Gallego ... [et al.] -- Oriented covalent immobilization of enzymes on heterofunctional-glyoxyl supports / Cesar Mateo ... [et al.] -- Reversible covalent immobilization of enzymes via disulfide bonds / Karen Ovsejevi, Carmen Manta, and Francisco Batista-Viera -- Immobilization of Candida rugosa lipase on superparamagnetic Fe3O4 nanoparticles for biocatalysis in low-water media / Joyeeta Mukherjee, Kusum Solanki, and Munishwar Nath Gupta -- Immobilization of enzymes by bioaffinity layering / Veena Singh, Meryam Sardar, and Munishwar Nath Gupta -- Immobilization of enzymes on magnetic beads through affinity interactions / Audrey Sassolas, Akhtar Hayat, and Jean-Louis Marty -- Tips for the functionalization of nanoparticles with antibodies / Ester Polo ... [et al.] -- Design and characterization of functional nanoparticles for enhanced bio-performance / Pablo del Pino, Scott G. Mitchell, and Beatriz Pelaz -- Immobilization of enzymes on ethynyl-modified electrodes via click chemistry / Akhtar Hayat ... [et al.] -- Modification of carbon nanotube electrodes with 1-pyrenebutanoic acid, succinimidyl ester for enhanced bioelectrocatalysis / Guinevere Strack ... [et al.] -- Enzyme immobilization by entrapment within a gel network / Audrey Sassolas, Akhtar Hayat, and Jean-Louis Marty -- Practical protocols for lipase immobilization via sol-gel techniques / Manfred T. Reetz -- Improving lipase activity by immobilization and post-immobilization strategies / Jose M. Palomo ... [et al.] -- High activity preparations of lipases and proteases for catalysis in low water containing organic solvents and ionic liquids / Ipsita Roy ... [et al.] -- Biomedical applications of immobilized enzymes : an update / Marta Pastor, Amaia Esquisabel, and José Luis Pedraz -- Immobilization of whole cells by chemical vapor deposition of silica / Susan R. Sizemore ... [et al.] -- Encapsulation of cells in alginate gels / Pello Sánchez ... [et al.] -- Microalgal immobilization methods / Ignacio Moreno-Garrido -- Therapeutic applications of encapsulated cells / Argia Acarregui ... [et al.] -- Whole cell entrapment techniques / Jorge A. Trelles and Cintia W. Rivero.
  • 2015 Springer Protocols
    edited by Adalberto Merighi and Laura Lossi, Department of Veterinary Sciences, University of Torino, Grugliasco, Torino, Italy.
    Machine generated contents note: 1.The Evolution of Immunocytochemistry in the Dissection of Neural Complexity / Laura Lossi -- pt. I Immunocytochemistry in Non-Mammalian Brain -- 2.Immunofluorescence and Genetic Fluorescent Labeling Techniques in the Drosophila Nervous System / Christian Wegener -- 3.Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris / Graziano Fiorito -- 4.Genetic Mosaic Labeling and Immunofluorescence Techniques in Zebrafish Brain / Yoshihiro Yoshihara -- pt. II Detection of Cell Proliferation and Neurogenesis -- 5.Immunocytochemical Phenotype of Differentiating Neurons / Antonio Carai -- 6.Immunocytochemical Identification of Mammalian Differentiating Neurons in the Process of Adult Neurogenesis in the Hippocampal Dentate Gyrus / Oliver von Bohlen und Halbach -- 7.Identification of Proliferating and Migrating Cells by BrdU and Other Thymidine Analogs: Benefits and Limitations / Pasko Rakic --
  • 2013 Springer
    Paul J. Fairchild, editor.
    Recent years have witnessed numerous advances in stem cell research that have suggested novel strategies for the treatment of the chronic and regenerative diseases that pose a growing challenge to the provision of healthcare in the 21<sup>st</sup> century. Nevertheless, there is little doubt that related disciplines have failed to keep pace with such developments, of which the immunology of transplant rejection is perhaps the most relevant to the nascent field of regenerative medicine. Indeed, given that after decades of research into the mechanisms of organ allograft rejection, we remain wholly dependent on immune suppression to ensure acceptance of foreign tissues, we might legitimately ask whether there is any reason why tissues differentiated from stem cells might not suffer the same fate. This volume seeks to address this issue by drawing on the principles of alloantigen recognition to assess the validity of early convictions that embryonic stem cell-derived tissues may prove invisible to the immune system of the host and the more recent belief that induced pluripotent stem cells, derived in a fully autologous manner, will circumvent the issue of immunogenicity. Having debunked such assumptions and established the magnitude of the immunological barriers to be overcome, this book then draws on the expertise of internationally-recognised laboratories around the world to provide a critical assessment of the likely success of applying strategies such as stem cell banking, silencing of genes that confer immunogenicity, and the induction of transplantation tolerance to overcoming the immunological barriers in the future. Indeed, it is only through reaching a consensus in the field that enduring solutions to such an intractable problem may ultimately emerge.
  • 2012 Springer
    Sibel Yildirim.
  • 2012 Springer
    by Vivi M. Heine, Stephanie Dooves, Dwayne Holmes, Judith Wagner.
  • 2011
    Kevin Shaoang Wei.
    1. VEGF inhibition increases hematocrit by hepatic Hif-2[Alpha]-dependent erythrocytosis and Hif-2[Alpha]--independent plasma volume contraction. Vascular Endothelial Growth Factor (VEGF) is an essential regulator of angiogenesis whose pharmacological inhibition represents a promising cancer therapeutic modality. We have previously reported that high-grade inhibition of VEGF by adenoviral expression of soluble VEGF receptor (VEGFR) ectodomains and recombinant VEGF Trap protein induce hepatic Epo-dependent erythrocytosis and polycythemia. Here, we demonstrate that VEGF inhibition induces erythrocytosis in a hepatic Hif-2[Alpha]-dependent manner. Conditional inactivation of hepatic Hif-2[Alpha] in Hif2[Alpha] flox/flox mice by either adenovirus Cre injection or albumin-Cre genetic cross suppressed VEGF inhibition-induced hepatic Epo production and blunted hematocrit elevation. Interestingly, a modest rise in hematocrit (5-10%) persisted in VEGF inhibitor-treated Hif-2[Alpha] liver knockout mice despite the absence of hepatic Epo elevation or reticulocytosis. Analysis of red blood cell mass and plasma volume indicate that, in addition to erythrocytosis, VEGF inhibition significantly reduces blood plasma volume (PV) (10-30% decrease) in a EPO- and Hif-2[Alpha]-independent manner. Thus, VEGF inhibition-induced hematocrit elevation in mice is attributable to both hepatic Hif-2[Alpha]-dependent erythrocytosis and Hif-2[Alpha] --independent plasma volume contraction, with accompanying implications for human patients exhibiting elevated haemoglobin levels in response to pharmacologic VEGF inhibition. 2. VEGF Inhibitors Enhance Hepatic insulin Signaling and Ameliorates Diabetes through Activation of Hepatic HIF-2[Alpha]. In the United States, over 18 million people have diabetes and approximately 90% of these cases are the type 2 form of the disease. The central pathophysiologic defect of T2DM is insulin resistance, where tissues such as muscle, fat and liver exhibit reduced responsiveness to insulin, despite normal to elevated levels of production of the hormone. During our studies on the role of vascular endothelial growth factor (VEGF) in erythropoeisis, we made a surprising observation that VEGF inhibition reduces blood glucose in normal lean mice and significantly corrects hyperglycemia in diabetic and insulin-resistant db/db mice. Metabolic profiling of VEGF inhibitor-treated mice revealed enhanced insulin sensitivity at the liver but not at the periphery. In the liver of these mice, expression of insulin receptor substrate-2 (IRS-2) was elevated, accompanied by enhanced Akt activation and suppression of gluconeogenic genes. Hepatic insulin sensitization by VEGF inhibitors requires hypoxia-inducible factor 2-alpha (Hif-2[Alpha]) as genetic deletion of hepatic Hif-2[Alpha] abrogated insulin sensitization and normalizes blood glucose in VEGF inhbitor-treated mice. Conversely, overexpression of a constitutively active allele of Hif-2[Alpha] in liver hepatocytes reduces blood glucose and activates hepatic insulin signaling in vitro and in vivo. Analysis of the human Irs-2 sequence revealed the presence of several potential Hypoxia-Responsive-Elements (HREs) within its 5' promoter. Using a Irs-2 promoter reporter construct, we found that overexpression of Hif-2[Alpha] or addition of a hypoxia-mimetic drug deferoxamine (DFO) resulted in increased Irs-2 transcription. These data suggest Irs-2 is a hypoxia-inducible gene. In this study, we show that VEGF inhibitors improve hepatic insulin action through activation of hepatic HIF-2[Alpha]. Our results suggest Hif-2[Alpha] positively regulates insulin signaling by promoting Irs-2 expression. These data suggest a previously unsuspected link between hypoxia and insulin signaling in the liver and indicate VEGF inhibitors may be useful for the treatment of diabetes.
  • 2016 Springer
    Seung-Kyu Han.
    1. Basics of wound healing -- 2. Interactive wound dressings -- 3. Biologic dermis graft -- 4. Tissue-engineered dermis graft -- 5. Atypical island flaps -- 6. Management of chronic wounds: with focus on diabetic ulcers -- 7. Infection, debridement, and biofilm -- 8. Negative-pressure wound therapy -- 9. Growth factor therapy -- 10. Cell therapy -- 11. Adjunctive therapy -- 12. Injectable tissue-engineered soft tissue.
  • 2013 CRCnetBASE
    Tony Fleming.
    1. Introduction -- 2. Self-field theory -- 3. Biological and cosmological evolution -- 4. Biophotons and diffusion in biology -- 5. Cell division and membrane diffusion -- 6. Electromagnetic and acoustic-based therapies.
  • 2008 Springer
    Michael Torzewski, Karl J. Lackner, Jürgen Bohl, Clemens Sommer.
  • 2011 Springer Protocols
    edited by Motomu Shimaoka.
    Overview : assays for studying integrin-dependent cell adhesion / Alexandre Chigaev and Larry A. Sklar -- Cell adhesion assays / Gabriele Weitz-Schmidt and Stephanie Chreng -- Real-time analysis of integrin-dependent transendothelial migration and integrin-independent interstitial motility of leukocytes / Ziv Shulman and Ronen Alon -- Lentiviral gene transfer method to study integrin function in T lymphocytes / Daliya Banerjee and Motomu Shimaoka -- Surface plasmon resonance biosensing in studies of the binding between b2 integrin I domains and their ligands / Thomas Vorup-Jensen -- Cell-free ligand-binding assays for integrin LFA-1 / Koichi Yuki -- Overview : structural biology of integrins / Guanyuan Fu, Wei Wang, and Bing-Hao Luo -- Protein expression and purification of integrin I domains and IgSF ligands for crystallography / Hongmin Zhang and Jia-huai Wang -- Electron microscopic imaging of integrin / Kenji Iwasaki -- NMR method to study protein-protein interactions / Noritaka Nishida and Ichio Shimada -- Single-molecule methods to study cell adhesion molecules / Joonil Seog -- Overview : imaging in the study of integrins / Christopher V. Carman -- Live imaging of LFA-1-dependent T-cell motility and stop signals / Andrew J. Wiemer, Sarah Wernimont, and Anna Huttenlocher -- Monitoring integrin activation by fluorescence resonance energy transfer / Craig T. Lefort, Young-Min Hyun, and Minsoo Kim -- High-resolution fluorescence microscopy to study transendothelial migration / Christopher V. Carman -- Multiphoton intravital microscopy to study lymphocyte motility in lymph nodes / Thomas T. Murooka and Thorsten R. Mempel -- Overview of integrin signaling in the immune system / Tatsuo Kinashi -- Rap1 and integrin inside-out signaling / Koko Katagiri and Tatsuo Kinashi -- Isolation of focal adhesion proteins for biochemical and proteomic analysis / Jean-Cheng Kuo [and others] -- Talin and signaling through integrins / Mohamed Bouaouina, David S. Harburger, and David A. Calderwood -- Proteomics method for identification of pseudopodium phosphotyrosine proteins / Yingchun Wang and Richard L. Klemke -- Overview : studying integrins in vivo / Clifford A. Lowell and Tanya N. Mayadas -- Method for the generation of conditional gene-targeted mice / Masahiro Yamamoto and Kiyoshi Takeda -- T-cell homing to the gut mucosa : general concepts and methodological considerations / Jaime De Calisto [and others] -- In vivo quantitative proteomics : the SILAC mouse / Sara Zanivan, Marcus Krueger, and Matthias Mann -- Analysis of chemotaxis in Dictyostelium / Huaqing Cai [and others] -- Integrins in tumor angiogenesis and lymphangiogenesis / Philippe Foubert and Judith A. Varner -- PET-radioimmunodetection of integrins : imaging acute colitis using a 6464Cu-labeled anti-B7 integrin antibody / Jason L.J. Dearling and Alan B. Packard -- Integrin-targeted nanoparticles for siRNA delivery / Noa Ben-Arie, Ranit Kedmi, and Dan Peer -- Humanized mice for studying human leukocyte integrins in vivo / Sang-Soo Kim [and others].
  • 2011 ClinicalKey
    editors-in-chief, Edward A. Dennis, Ralph A. Bradshaw.
    "Cell signaling, which is also often referred to as signal transduction or, in more specialized cases, transmembrane signaling, is the process by which cells communicate with their environment and respond temporally to external cues that they sense there. All cells have the capacity to achieve this to some degree, albeit with a wide variation in purpose, mechanism, and response. At the same time, there is a remarkable degree of similarity over quite a range of species, particularly in the eukaryotic kingdom, and comparative physiology has been a useful tool in the development of this field. The central importance of this general phenomenon (sensing of external stimuli by cells) has been appreciated for a long time, but it has truly become a dominant part of cell and molecular biology research in the past three decades, in part because a description of the dynamic responses of cells to external stimuli is, in essence, a description of the life process itself. This approach lies at the core of the developing fields of proteomics and metabolomics, and its importance to human and animal health is already plainly evident"--Provided by publisher.
  • 2011 Springer
    Aleš Prokop, editor.
  • 2015 Springer
    Isabella C. Felli, Roberta Pierattelli, editors.
    1. Back to the future: nuclear magnetic resonance and bioinformatics studies on intrinsically disordered proteins -- 2. Structure and dynamics of intrinsically disordered proteins -- 3. NMR methods for the study of intrinsically disordered proteins structure, dynamics, and interactions: general overview and practical guidelines -- 4. Ensemble calculation for intrinsically disordered proteins using NMR parameters -- 5. NMR spectroscopic studies of the conformational ensembles of intrinsically disordered proteins -- 6. Recombinant intrinsically disordered proteins for NMR: tips and tricks -- 7. Biophysical methods to investigate intrinsically disordered proteins: avoiding an "elephant and blind men" situation -- 8. Application of SAXS for the structural characterization of IDPs -- 9. Bioinformatics approaches for predicting disordered protein motifs -- 10. Towards understanding protein disorder in-cell -- 11. The Protein Ensemble Database -- 12. Order and disorder in the replicative complex of paramyxoviruses -- 13. Druggability of intrinsically disordered proteins -- 14. Beta amyloid hallmarks: from intrinsically disordered proteins to Alzheimer's Disease.
    Also available: Print – 2015
  • 2013 ScienceDirect
    William Stillwell.
    An Introduction to Biological Membranes: From Bilayers to Rafts covers many aspects of membrane structure/function that bridges membrane biophysics and cell biology. Offering cohesive, foundational information, this publication is valuable for advanced undergraduate students, graduate students and membranologists who seek a broad overview of membrane science. Brings together different facets of membrane research in a universally understandable mannerEmphasis on the historical development of the field. Topics include membrane sugars, membrane models, membrane isolation methods, and membrane transport.
  • 2008 ScienceDirect
    edited by Terence D. Allen.
    Also available: Print – 2008
  • 2014 Springer
    Ricardo H. Bardales, editor.
    Chapter 1. Ultrasound, general considerations -- Chapter 2. The interventional cytopathologist -- Chapter 3. The thyroid gland -- Chapter 4. The parathyroid gland -- Chapter 5. The salivary glands -- Chapter 6. The head and neck, miscellaneous lesions -- Chapter 7. The lymph nodes -- Chapter 8. The breast -- Index.
  • 2012
    Angela N. Bowman.
    In order for a tissue to be properly sized and patterned, enormous care must be taken to ensure that cell proliferation and differentiation are tightly regulated. The embryonic brain must execute a temporally and spatially defined developmental scheme, resulting in the production of millions of cells of multiple lineages. These cells and their resultant functional architecture must be maintained in the homeostatic adult mouse brain. These processes are in part controlled by external signaling factors, which include Wnts, and their transcriptional programs. However, the degree to which and exact mechanisms by which cell fate choices--including self-renewal, proliferation, and differentiation--are controlled by Wnt/ss-catenin signaling are unknown. Here, I describe studies to investigate these questions. First, I show the identification of a novel target of Wnt/sscatenin signaling, Pou6f1, which mediates neural differentiation and proliferation in embryonic neural progenitor cells. Next, I illustrate the use of a lineage tracing approach using a Wnt/ss-catenin target gene, Axin2, to identify and trace sites of Wnt/ss-catenin responsiveness in the developing and adult mouse brain and central nervous system. Using this same Axin2CreERT2 mouse, I describe the functional consequences of loss of sscatenin from Axin2-expressing cells. Axin2CreERT2 labels numerous radial glial cell populations throughout the central nervous system, three of which serve as functional stem/progenitor populations. Loss of ss-catenin from adult Axin2+ populations results in tissue architecture defects and increased proliferation, presumably because Wnt serves as a self-renewal signal. These studies demonstrate the importance of Wnt/ss-catenin signaling for mediating multiple cell fate choices during the development and homeostasis of the mouse central nervous system, and suggest mechanisms by which neural stem/progenitor populations could be manipulated for therapeutic outcomes.
  • 2014 CRCnetBASE
    edited by Matthew Eckwahl, MPhil.
    Part 1. Cell polarity, growth, and cell cycle -- part 2. Yeast regulatory circuits -- part 3. Gene expression and trait locus analysis -- part 4. Comparative genomics.
  • 1996 ScienceDirect
    Edward C. Conley ; with contributions from William J. Brammar.
    1. Extracellular ligand-gated channels -- 2. Intracellular ligand-gated channels -- 3. Inward rectifier and intercellular channels -- 4. Voltage-gated channels.
  • 2013 Springer Protocols
    edited by Nikita Gamper.
    1. Approaches to cloning of pain-related ion channel genes / Armen N. Akopian -- 2. Mammalian expression systems and transfection techniques / Daunia Laurenti and Lezanne Ooi -- 3. Use of Escherichia coli for the production and purification of membrane proteins / Vincent G.L. Postis, Andrea E. Rawlings, Amelia Lesiuk, and Stephen A. Baldwin -- 4. Transient overexpression of genes in neurons using nucleofection / Hannah M. Kirton, Louisa Pettinger, and Nikita Gamper -- 5. Viral gene delivery : optimized protocol for production of high titer lentiviral vectors / James Hewinson, Julian F.R. Paton, and Sergey Kasparov -- 6. Two-electrode voltage clamp / Bingcai Guan, Xingjuan Chen and Hailin Zhang -- 7. Conventional micropipette-based patch clamp techniques /Jonathan D. Lippiat and David C. Wrighton -- 8. Recording of ion channel activity in planar lipid bilayer experiments / Eleonora Zakharian -- 9. Recording macroscopic currents in large patches from Xenopus oocytes / Tibor Rohacs -- 10. Combined single-channel and macroscopic recording techniques to analyze gating mechanisms of the large conductance Ca²⁺ and voltage activated (BK) potassium channel / Nguyen V. Nguyen, Aleksandra Gruslova, Wojciech A. Kosiba and Bing Wang -- 11. Perforated whole-cell patch-clamp recording / John E. Linley -- 12. Piezo-electrically driven mechanical stimulation of sensory neurons / Jizhe Hao, Jérôme Ruel, Bertrand Coste, Yann Roudaut, Marcel Crest and Patrick Dlemas -- 13. Automated planar patch-clamp / Carol J. Milligan and Clemens Möller -- 14. Recording single-channel currents using "smart patch-clamp" technique / Anamika Bhargava and Julia Gorelik -- 15. Using total internal reflection fluorescence microscopy to observe ion channel trafficking and assembly / Sarah Schwarzer, Gregory I. Mashanov, Justin E. Molloy, and Andrew Tinker -- 16. Förster resonance energy transfer-based imaging at the cell surface of live cells / Sonya M. Bierbower and Mark S. Shapiro -- 17. Use of dansyl-calmodulin to study interactions with channels and other proteins / Alessandro Alaimo, Covadonga Malo, Pilar Areso, Kerman Aloria, Oscar MIllet and Alvaro Villarroel -- 18. Imaging and quantification of recycled K[subscript ATP] channels / Christopher J. Cockcroft -- 19. Generation of antibodies that are externally acting isoform-specific inhibitors of ion channels / Jacqueline Naylor and David J. Beech -- 20. Site-directed mutagenesis to study the structure-function relationships of ion channels / Wei Yang and Lin-Hua Jiang -- 21. Cysteine-based cross-linking approach to study inter-domain interactions in ion channels / Lin-Hua Jiang -- 22. Analysis of Ca²⁺⁻ binding sites in the MthK RCK domain by x-ray crystallography / Frank J. Smith and Brad S. Rothberg -- 23. Isotope labeling strategies for analysis of an ion channel cytoplasmic domain by NMR spectroscopy / Karin Abarca-Heidemann, Elke Duchardt-Ferner, Jens Woehnert and Brad S. Rothberg -- 24. Recording dendritic ion channel properties and function from cortical neurons / Mala M. Shah -- 25. M-current recording from acute DRG slices / Kirstin E. Rose, Sylvain Gigout, and Nikita Gamper -- 26. Studying ion channels in human erythrocytes by direct and indirect means / Stephan M. Huber -- 27. Recording ion channels in isolated, split-opened tubules / Elena Mironova, Vladislav Bugay, Oleh Pochynyuk, Alexander Staruschenko and James D. Stockand -- 28. Single-channel analysis of TRPC channels in the podocytes of freshly isolated glomeruli / Daria V. Ilatovskaya and Alexander Staruschenko -- 29. Ca²+ imaging as a tool to assess TRP channel function in murine distal nephrons / Mykola Mamenko, Oleg Zaika, Roger G. O'Neil and Oleh Pochynyuk -- 30. Patch-clamping Drosophila sensory neurons / Volodymyr Kucher, Benjamin A. Eaton, James D. Stockand and Nina Boiko -- 31. Production and validation of recombinant adeno-associated virus for channelrhodopsin expression in neurons / John Y. Lin -- 32. Optical control of ligand-gated ion channels / Stephanie Szobota, Catherine McKenzie, and Harald Janovjak.
  • 2012
    Feng-Chiao Tsai.
    Terminating cell migration is critical for cancer treatment, because malignancy progresses through migrating tumor cells (metastasis) and migrating endothelial cells (neoangiogenesis.) Although cell migration requires Ca2+ for polarity and turning, it is not well understood how Ca2+ signals regulate cell migration. By studying sheet migration using endothelial cells loaded with Fura-2/AM, we identified pulsatile local Ca2+ pulses near the cell front. Each Ca2+ pulse controlled a local retraction and adhesion process in lamellipodia. Eliminating endogenous Ca2+ pulses reduced lamellipodial retraction while photo-release of Ca2+ from NP-EGTA induced front retraction and adhesion, indicating that these small Ca2+ signals were both necessary and sufficient for cyclic lamellipodia activity. Mechanistically, these local Ca2+ pulses acts through myosin light-chain kinase and myosin II to mediate actin cable retraction and to strengthen focal adhesion. We further explored the origin of these local Ca2+ signals. Removing extracellular Ca2+ did not eliminate local Ca2+ pulses, suggesting that these local signals are generated from activated inositol-3-phosphate receptors (IP3R) in the endoplasmic reticulum (ER). Inhibiting receptor tyrosine kinases (RTK) diminished local Ca2+ pulses, confirming that RTK-induced IP3R activation was the major source of these Ca2+ signals. More experiments demonstrated that diacylglycerol, the downstream regulator of RTK signaling, was also enriched in the cell front. It contributed to cell motility and polarity through protein kinase C. Therefore, polarized RTK signaling generates local Ca2+ signals and diacylglycerol in the cell front to ensure coordinated cell movement. We also investigated how Ca2+ homeostasis was maintained during cell migration. Our data showed that plasma membrane Ca2+ ATPases (PMCA) pumped Ca2+ out of the cell more actively in the front than in the back. The resulting low basal Ca2+ in the front of migrating cells maintained the sensitivity of the cell front to local Ca2+ pulses. Furthermore, to avoid depletion of Ca2+ in the front ER after it being released through IP3R, microtubules transported STIM1, a sensor of ER luminal Ca2+, toward the cell front triggering store-operated Ca2+ influx near the leading edge. Thus, cells maintain efficient migration through coordinated Ca2+ signaling systems. In summary, temporal-spatial coordination of Ca2+ signaling controls cell migration by regulating local adhesion, motility, and polarization. Our research also reveals the importance of interactions between signaling modules in migrating cells. Finally, a "synthetic immobility" screen will elucidate how different components are coordinated in cell migration, and will identify novel drug targets against cancer metastasis.
  • 2012 Springer
    Thomas Decker, Mathias Müller, editors.
    The Continuing Fascination with Jaks and Stats: An Introduction / Thomas Decker and Mathias Müller -- Regulation of JAKs: Insights Gleaned from the Functional Protein Domains / Claude Haan, Daniela Ungureanu, Tuija Pekkala, Olli Silvennoinen and Serge Haan -- Nuclear Functions of the Janus Kinases / Mark A. Dawson and Andrew J. Bannister -- Self-association of STAT Proteins from Monomers to Paracrystals / Mathias Droescher and Uwe Vinkemeier -- How Stats Interact with the Molecular Machinery of Transcriptional Activation / Amanda M. Jamieson, Matthias Farlik and Thomas Decker -- Nongenomic Functions of STAT3 / Daniel J. Gough, Pravin Sehgal and David E. Levy -- Evolution of Jak and Stat Proteins / Claus Vogl, Priyank Shukla and Ingo Ebersberger -- JAK/STAT and Chromatin Regulation in Drosophila / Kriti Gaur and Willis X. Li -- JAK/STAT Signaling and Invertebrate Immune Responses / Feng Zhou and Hervé Agaisse -- Stat5 as a Hematopoietic Master Regulator for Differentiation and Neoplasia Development / Harini Nivarthi, Katrin Friedbichler and Richard Moriggl -- Regulation of Dendritic Cell Development by STATs / Haiyan S. Li and Stephanie S. Watowich. STAT Transcription Factors: Controlling All Aspects of NK Cell Biology / Eva Maria Putz, Eva Zebedin and Veronika Sexl -- Function of JAKs and STATs in Lymphocytes: Bench to Bedside / Alejandro Villarino, Xiang-Ping Yang, Kiyoshi Hirahara, Yuka Kanno and John J. O' Shea, et al. -- The Role of Janus Kinases in Hematopoietic Malignancies / Damla Olcaydu and Robert Kralovics -- Activation of Janus Kinases During Tumorigenesis / Jeffrey W. Schmidt and Kay-Uwe Wagner -- STAT1 and STAT3 Transcription Factors in Inflammation-associated Colon Cancer / Paulina Rampetsreiter and Robert Eferl -- Universal and Specific Functions of STAT3 in Solid Tumours / Lidia Avalle, Gabriella Regis and Valeria Poli -- Impact of STAT5 on Normal Tissue Development and Cancer / Maria M. Caffarel and Christine J. Watson -- Crosstalk with the Jak-STAT Pathway in Inflammation / Lionel B. Ivashkiv -- Activation and Inhibition of JAK-STAT Signal Transduction by RNA Viruses / Curt M. Horvath -- Multifaceted Antiviral Actions of Interferon-stimulated Gene Products / Birgit Strobl, Nicole R. Leitner and Mathias Müller -- Interferon Regulation of the Innate Response to Bacteria / Ali Abdul-Sater and Christian Schindler.
  • 2013 Springer Protocols
    edited by Sandra E. Nicholson and Nicos A. Nicola.
    Analysis of Janus tyrosine kinase phosphorylation and activation / Jeremy A. Ross, Georgialina Rodriguez, and Robert A. Kirken -- Co-immunoprecipitation protocol to investigate cytokine receptor-associated proteins, e.g., Janus kinases or other associated signaling proteins / Claude Haan and Serge Haan -- In vitro JAK kinase activity and inhibition assays / Jeffrey J. Babon and James M. Murphy -- Quantitative analysis of JAK binding using isothermal titration calorimetry and surface plasmon resonance / Jeffrey J. Babon -- Determination of protein turnover rates in the JAK/STAT pathway using a radioactive pulse-chase approach / Anna Dittrich, Elmar Siewert, and Fred Schaper -- Designing RNAi screens to identify JAK/STAT pathway components / Katherine H. Fisher, Stephen Brown, and Martin P. Zeidler -- The use of JAK-specific inhibitors as chemical biology tools / Christopher J. Burns, David Segal, and Andrew F. Wilks -- Methods for detecting mutations in the human JAK2 gene / Anthony J. Bench, E. Joanna Baxter, and Anthony R. Green -- Detection of activated STAT proteins / Jean-Patrick Parisien and Curt M. Horvath -- Detection of activated STAT species using electrophoretic mobility shift assay (EMSA) and potential pitfalls arising from the use of detergents / Serge Haan and Claude Haan -- Flow cytometric analysis of STAT phosphorylation / Jane Murphy and Gabrielle L. Goldberg -- Acetylation of endogenous STAT proteins / Torsten Ginter, Thorsten Heinzel, and Oliver H. Krämer -- Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining / Reza Khorooshi and Trevor Owens -- Nuclear trafficking of STAT proteins visualized by live cell imaging / Velasco Cimica and Nancy C. Reich -- Characterization of STAT self-association by analytical ultracentrifugation / Nikola Wenta and Uwe Vinkemeier -- Constitutively active STAT5 constructs / Lynn M. Heltemes-Harris and Michael A. Farrar -- Analysis of suppressor of cytokine signalling (SOCS) gene expression by real-time quantitative PCR / Tatiana B. Kolesnik and Sandra E. Nicholson -- Detection of endogenous SOCS1 and SOCS3 proteins by immunoprecipitation and western blot analysis / Jian-Guo Zhang and Sandra E. Nicholson -- In vitro ubiquitination and cytokine signaling components / Jeffrey J. Babon, Artem Laktyushin, and Nadia J. Kershaw -- Production and crystallization of recombinant JAK proteins / Isabelle S. Lucet and Rebecca Bamert -- Bacterial expression, purification, and crystallization of tyrosine phosphorylated STAT proteins / Florence Baudin and Christoph W. Müller.
  • 2009 Springer
    Peter De Wulf, William C. Earnshaw, editors.
    Centromeres and kinetochores : an historical perspective / Kerry S. Bloom -- The basics of chromosome segregation / Mitsuhiro Yanagida -- The centromere / Beth A. Sullivan -- Neocentromeres / Owen J. Marshall and K.H. Andy Choo -- Human artificial centromeres : de novo assembly of functional centromeres on human artificial chromosomes / Hiroshi Masumoto, Teruaki Okada, and Yasuhide Okamoto -- Kinetochore composition, formation, and organization / Tatsuo Fukagawa and Peter De Wulf -- Evolution of centromeres and kinetochores : a two-part fugue / Paul B. Talbert, Joshua J. Bayes, and Steven Henikoff -- Mitotic spindle assembly mechanisms / Rebecca Heald and Claire E. Walczak -- Kinetochore-microtubule interactions / Lesley Clayton and Tomoyuki U. Tanaka -- Post-translational modifications that regulate kinetochore activity / Chitra V. Kotwaliwale and Sue Biggins -- The role of the kinetochore in spindle checkpoint signaling / P. Todd Stukenberg and Daniel J. Burke -- Kinetochore regulation of anaphase and cytokinesis / Scott Thomas and Kenneth B. Kaplan -- Roles of centromeres and kinetochores in meiosis / Adele L. Marston -- The kinetochore-cancer connection / Takeshi Tomonaga -- The kinetochore as target for cancer drug development / Song-tao Liu and Tim J. Yen.
  • 2013 Springer
    Alexander S. Kauffman, Jeremy T. Smith, editors.
    Also available: Print – 2013
  • 2012 Springer
    Leopold G Koss, Rana S. Hoda.
    Indication, Collection, and Laboratory Processing of Cytologic Samples -- The Cellular and Acellular Components of the Urinary Sediment -- The Cytologic Makeup of the Urinary Sediment According to the Collection Technique -- Cytologic Manifestations of Benign Disorders Affecting Cells of the Lower Urinary Tract -- Tumors and Related Conditions of the Bladder and Lower Urinary Tract -- Urine-Based Assays Complementing Cytologic Examination in the Detection of Urothelial Neoplasm.
  • 2012 ScienceDirect
    [edited by] P. Michael Conn.
    Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields.
  • 2013 ScienceDirect
    [edited by] P. Michael Conn.
    Structural dynamics of nucleic acids by single-molecule FRET / Asger Christian Krüger, Lasse Lava Hildebrandt, Sofie Louise Kragh, Victoria Birkedal -- Visualizing the location and the dynamics of gene expression in living animals through bioluminescence imaging / Xiaojuan Chen -- Quality control protocol for confocal system / Anda Cornea -- Electron microscopy of bone / V. Everts, K.A. Hoeben -- Immunostaining : detection of signaling protein location in tissues, cells and subcellular compartments / Biswanath Maity, David Sheff, Rory A. Fisher -- Bimolecular fluorescence complementation (BiFC) analysis of protein-protein interaction : how to calculate signal-to-noise ratio / Yutaka Kodama, Chang-Deng Hu -- Immunohistochemical detection of neuropeptides/transmitters in mammalian brain / Lothar Jennes -- Intravital imaging of autoreactive T. cells in living animals / Naoto Kawakami, Ingo Bartholomäus, Marija Pesic, Nikolaos I. Kyratsous -- Genetically encoded click chemistry for single-molecule FRET of proteins / Swati Tyagi, Edward A. Lemke -- High-throughput methods for the detection of protein overexpression using fluorescence markers / Mads Gabrielsen, Andrew J. Roe, Karen McLuskey -- Correlative microscopy / Alexander A. Mironov, Galina V. Beznoussenko -- Stereology of the lung / Jan Philipp Schneider, Matthias Ochs -- Quantitative 3D cell-based assay performed with cellular spheroids and fluorescence microscopy / Nariman Ansari, Stefanie Müller, Ernst H.K. Stelzer, Francesco Pampaloni -- Optimizing fluorescence excitation and detection for intravital two-photon microscopy / Dan Suan, Henry R. Hampton, Michio Tomura, Osami Kanagawa, Tatyana Chtanova, Tri Giang Phan -- Two-dimensional crystallization by dialysis for structural studies of membrane proteins by the cryo-EM method electron crystallography / Matthew C. Johnson, Ingeborg Schmidt-Krey -- In situ hybridization / Chung-Jui Tsai, Scott A. Harding -- RNA imaging : tracking in real-time RNA transport in neurons using molecular beacons and confocal microscopy / Angélica Zepeda, Clorinda Arias, Fabian Flores-Jasso, Luis Vaca.
  • 2012 Springer
    Yoshiharu Takayama.
    "Lactoferrin was first identified as an iron-binding glycoprotein abundantly found in milk. It has been considered to play important roles in host defense, since lactoferrin shows broad spectrum of anti-bacterial and anti-viral activities, arising from its iron-chelating property. Furthermore, lactoferrin can regulate the function of innate and adaptive immune cells and exhibits immuno-modulating properties. These activities of lactoferrin implicate that potential use of lactoferrin as a wound healing agent. In addition, this book shows that lactoferrin is a potent regulator of dermal fibroblasts and epidermal keratinocytes, and promotes skin or corneal epithelial wound healing by increasing their proliferation, migration or deposition of extracellular matrix (ECM) components such as collagen and hyaluronan. This book provides the scientific background on use of lactoferrin as a wound healing agent. Indeed, lactoferrin exerts its biological effects by binding to specific lactoferrin receptors on target cells. This book also covers the in-depth information on roles of lactoferrin as a signaling molecule."--Publisher's website.
  • 2007 ScienceDirect
    edited by Michael W. Berns, Karl Otto Greulich.
    Also available: Print – 2007
  • 2014 Springer
    edited by Shizuya Saika, Liliana Werner, Frank J. Lovicu.
    This book is the first to summarize the current knowledge of the cell biology of lens epithelial cells in relation to and in the development of posterior capsular opacification (PCO). PCO remains the most common long-term complication of modern cataract surgery, occurring months or years after cataract surgery, unlike most other complications that tend to arise during or soon after the procedure. Opacification of the posterior capsule appears to be linked to lens epithelial cells that are left behind in the eye during cataract removal. These cells proliferate, migrate across the posterior lens capsule, and undergo changes that result in fibrous or pearl-type opacities in the capsule. The first section of the text explains the molecular mechanism and biology of lens epithelial cells that lead to the incidence of PCO. In the second part, in addition to a description of the mechanism and pathological condition of PCO, surgical methods and devices for preventing PCO are discussed in detail. Lens Epithelium and Capsular Opacification will benefit not only young clinical residents and junior researchers, but also established faculty in the clinical or basic academic field.
  • 2012 Springer Protocols
    edited by Robert B. Ashman.
    ENU-based phenotype-driven screening / Vera M. Ripoll, Philip L. Kong, and Paul K. Potter -- Detection and quantification of cytokines and other biomarkers / Evan L. Chiswick ... [et al.] -- Flow cytometry analysis of cell cycling and proliferation in mouse hematopoietic stem and progenitor cells / Valérie Barbier ... [et al.] -- Flow cytometry measurement of bone marrow perfusion in the mouse and sorting of progenitors and stems cells according to position relative to blood flow in vivo / Valérie Barbier ... [et al.] -- Analyzing cell death events in cultured leukocytes / Karin Christenson, Fredrik B. Thorén, and Johan Bylund -- Towards a four-dimensional view of neutrophils / Ben A. Croker, Andrew W. Roberts, and Nicos A. Nicola -- Isolation of human and mouse neutrophils ex vivo and in vitro / Yan Hu -- Measurement of oxidative burst in neutrophils / Yu Chen and Wolfgang G. Junger -- Measurement of neutrophil elastase, proteinase 3, and cathepsin G activities using intramolecularly quenched fluorogenic substrates / Brice Korkmaz ... [et al.] -- The macrophage / Chris P. Verschoor, Alicja Puchta, and Dawn M.E. Bowdish -- Generation and characterization of MacGreen mice, the CFS1R-EGFP transgenic mice / R. Tedjo Sasmono and Elizabeth Williams -- Generation of mouse bone marrow-derived macrophages / Silvia Manzanero -- Isolation and differentiation of monocytes-macrophages from human blood / Dipti Vijayan -- In vitro measurement of phagocytosis and killing of Cryptococcus neoformans by macrophages / André Moraes Nicola and Arturo Casadevall -- Measuring the inflammasome / Olaf Gross -- Arginine and macrophage activation / Mònica Comalada -- Immunodetection of granzyme B tissue distribution and cellular localisation / Catherina H. Bird ... [et al.] -- Detection of human and mouse granzyme B activity in cell extracts / Sarah Elizabeth Stewart ... [et al.] -- T cell transfer model of colitis : a great tool to assess the contribution of T cells in chronic intestinal inflammation / Rajaraman Eri, Michael A. McGuckin, and Robert Wadley -- Measurement of nitrite in urine by gas chromatography-mass spectrometry / Dimitrios Tsikas ... [et al.].
  • 2012
    Luis Alberto Chia.
    The intestinal epithelium is one of the most rapidly proliferating tissues in the body. A complete turnover of the epithelium occurs every 3-5 days in the mouse, a process that is maintained by a small population of intestinal stem cells (ISCs) that reside in the crypt bases. The signals that regulate the behavior of these ISCs are still poorly understood. However, the recent identification of genes that mark functional stem cells has yielded insights into how ISCs are regulated and maintained. Here, we demonstrate that Bmi1 and Lgr5 mark two functionally distinct ISCs in vivo. Lgr5 marks mitotically active ISCs that exhibit exquisite sensitivity to canonical Wnt modulation, contribute robustly to homeostatic regeneration, and are quantitatively ablated by irradiation. In contrast, Bmi1 marks quiescent ISCs that are insensitive to Wnt perturbations, contribute weakly to homeostatic regeneration, and are resistant to high dose radiation injury. Post-irradiation, however, the normally quiescent Bmi1+ ISCs dramatically proliferate to clonally repopulate multiple contiguous crypts and villi. Clonogenic culture of isolated single Bmi1+ ISCs yields long-lived self-renewing spheroids of intestinal epithelium that produce Lgr5-expressing cells, thereby establishing a lineage relationship between these two populations in vitro. Taken together, these data provide direct evidence that Bmi1 marks quiescent, injury-inducible reserve ISCs that exhibit striking functional distinctions from Lgr5+ ISCs, and support a model whereby distinct ISC populations facilitate homeostatic versus injury-induced regeneration. How these ISCs maintain their stemness remains unclear. Paneth cells have been suggested to serve as niche cells for the Lgr5+ ISCs, perhaps through the secretion of essential paracrine factors, but recent reports clearly demonstrate that Paneth cells are not required for Lgr5+ ISC maintenance. Recently, the G-protein-coupled receptors Lgr4-6 were reported to associate with Wnt receptors to mediate R-spondin signaling. Given the importance of Wnt/R-spondin signaling in intestinal crypt maintenance, we tested the in vivo function of Lgr5 by adenovirus-mediated overexpression of the soluble ligand-binding Lgr5 extracellular domain. Circulating Lgr5 ectodomain induced the migration of Paneth cells from the crypts, their eventual loss, and a concomitant disappearance of Lgr5+ ISCs. Paneth cell migration was associated with downregulation of Wnt signaling and its target EphB3. The loss of Lgr5+ ISCs did not affect maintenance of the intestinal epithelium, nor did Lgr5+ stem cells disappear from non-intestinal organs. Together, these findings characterize an easily tractable experimental model for the in vivo deletion of Lgr5+ ISCs, and suggest that Lgr receptors function to actively maintain Lgr5+ ISCs in vivo.
  • 2011 Springer Protocols
    edited by Hélio Chiarini-Garcia, Rossana C.N. Melo.
    Glycol methacrylate embedding for improved morphological, morphometrical, and immunohistochemical investigations under light microscopy : testes as a model / Hélio Chiarini-Garcia, Gleydes Gambogi Parreira, and Fernanda R.C.L. Almeida -- Histological processing of teeth and periodontal tissues for light microscopy analysis / Gerluza Aparecida Borges Silva, Adriana Moreira, and José Bento Alves -- Large plant samples: how to process for GMA embedding / Élder Antônio Sousa Paiva, Sheila Zambello de Pinho, and Denise Maria Trombert Oliveira -- Image cytometry : nuclear and chromosomal DNA quantification / Carlos Roberto Carvalho, Wellington Ronildo Clarindo, and Isabella Santiago Abreu -- Histological approaches to study tissue parasitism during the experimental Trypanosoma cruzi infection / Daniela L. Fabrino, Grazielle A. Ribeiro, Livia Teixeira, and Rossana C.N. Melo -- Intravital microscopy to study leukocyte recruitment in vivo / Vanessa Pinho ... [et al.] -- Introduction to fluorescence microscopy / Ionita C. Ghiran -- Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals / Ernani Amaral, Silvia Guatimosim, and Cristina Guatimosim -- Imaging lipid bodies within leukocytes with different light microscopy techniques / Rossana C.N. Melo ... [et al.] -- Eicosa cell : an immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites / Christianne Bandeira-Melo, Peter F. Weller, and Patricia T. Bozza -- Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer / Robert M. Hoffman -- Imaging calcium sparks in cardiac myocytes / Silvia Guatimosim, Cristina Guatimosim, and Long-Sheng Song -- Light microscopy in aquatic ecology: methods for plankton communities studies / Maria Carolina S. Soares ... [et al.] -- Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedded samples / Shin-ichi Iwasaki and Hidekazu Aoyagi.
  • 2013 ScienceDirect
    edited by Hongyuan Yang and Peng Li
    This new volume of Methods in Cell Biology looks at lipid droplets LDs, covering sections on analyses of LDs in model systems, cell/tissue-specific analyses of LDs and imaging and in vitro analyses of LD biogenesis and growth. Chapters are written by experts in the field. With cutting-edge material, this comprehensive collection is intended to guide researchers of LDs for years to come. Covers sections on analyses of lipid droplets (LDs) in model systems, cell/tissue-specific analyses of LDs and imaging, and in vitro analyses of LD biogenesis and growth Chapters are written by experts in t
  • 2016 Springer Protocols
    edited by Mark Waugh.
    Method for assaying the lipid kinase phosphatidylinositol-5-phosphate 4-kinase α in quantitative high-throughput screening (qHTS) bioluminescent format / Mindy I. Davis, Atsuo T. Sasaki, and Anton Simeonov -- Assaying ceramide synthase activity in vitro and in living cells using liquid chromatography-mass spectrometry / Xin Ying Lim, Russell Pickford, and Anthony S. Don -- Fluorescent assays for ceramide synthase activity / Timothy A. Couttas and Anthony S. Don -- Identification of the interactome of a palmitoylated membrane protein, phosphatidylinositol 4-kinase type II alpha / Avanti Gokhale ... [et al.] -- Measurement of long-chain fatty Acyl-CoA synthetase activity / Joachim Füllekrug and Margarete Poppelreuther -- Qualitative and quantitative in vitro analysis of phosphatidylinositol phosphatase substrate specificity / Laura Ren Huey Ip and Christina Anja Gewinner -- Luciferase reporter assays to assess liver X receptor transcriptional activity / Matthew C. Gage, Benoit Pourcet, and Inés Pineda-Torra -- Metabolically biotinylated reporters for electron microscopic imaging of cytoplasmic membrane microdomains / Kimberly J. Krager and John G. Koland -- Fluorescence recovery after photobleaching analysis of the diffusional mobility of plasma membrane proteins : HER3 mobility in breast cancer cell membranes / Mitul Sarkar and John G. Koland -- Isolation and analysis of detergent-resistant membrane fractions / Massimo Aureli ... [et al.] -- Detection of isolated mitochondria-associated ER membranes using the sigma-1 receptor / Abasha Lewis, Shang-Yi Tsai, and Tsung-Ping Su -- Using surface plasmon resonance to quantitatively assess lipid-protein interactions / Kathryn Del Vecchio and Robert V. Stahelin -- Analyzing protein-phosphoinositide interactions with liposome flotation assays / Ricarda A. Busse ... [et al.] -- High-throughput fluorometric assay for membrane-protein interaction / Wonhwa Cho, Hyunjin Kim, and Yusi Hu -- Guidelines for the use of protein domains in acidic phospholipid imaging / Matthieu Pierre Platre and Yvon Jaillais -- Analysis of sphingolipid synthesis and transport by metabolic labeling of cultured cells with [3H]serine / Neale D. Ridgway -- Determination and characterization of tetraspanin-associated phosphoinositide-4 kinases in primary and neoplastic liver cells / Krista Rombouts and Vinicio Carloni -- Analysis of the phosphoinositide composition of subcellular membrane fractions / Deborah A. Sarkes and Lucia E. Rameh -- Single-molecule imaging of signal transduction via GPI- anchored receptors / Kenichi G. N. Suzuki -- Measuring phosphatidylinositol generation on biological membranes / Mark Waugh -- Assay for CDP-diacylglycerol generation by CDS in membrane fractions / Mark Waugh.
  • 2013 Springer
    Daniel G.S. Capelluto, editor.
    Sphingomyelin and its role in cellular signaling / Mahua Chakraborty and Xian-Cheng Jiang -- The driving force of alpha-synuclein insertion and amyloid channel formation in the plasma membrane of neural cells: key role of ganglioside- and cholesterol-binding domains / Jacques Fantini and Nouara Yahi -- The enigmatic role of sulfatides: new insights into cellular functions and mechanisms of protein recognition / Shuyan Xiao, Carla V. Finkielstein, and Daniel G. S. Capelluto -- Phosphoinositides and PDZ domain scaffolds / Anna Maria Wawrzyniak, Rudra Kashyap and Pascale Zimmermann -- PtdIns(4)P signalling and recognition systems / Marc Lenoir and Michael Overduin -- PtdIns(4,5)P2-mediated cell signaling: emerging principles and PTEN as a paradigm for regulatory mechanism / Arne Gericke, Nicholas R. Leslie, Mathias Lösche, and Alonzo H. Ross -- Role of phosphatidylinositol 3,4,5-trisphosphate in cell signaling / Robert D Riehle, Sinziana Cornea and Alexei Degterev -- The role of phosphoinositides and inositol phosphates in plant cell signaling / Glenda E. Gillaspy -- Phosphatidic acid-mediated signaling / Yu Liu, Yuan Su, and Xuemin Wang -- Phosphatidylserine-mediated cellular signaling / Jason G. Kay and Sergio Grinstein -- Cardiolipin-mediated cellular signaling / Vinay A. Patil and Miriam L. Greenberg.
    Also available: Print – 2013
  • 2012 ScienceDirect
    edited by Gilbert Di Paolo, Markus R. Wenk.
    Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding / Min Wu, Pietro de Camilli -- Studying lipids involved in the endosomal pathway / Christin Bissig, Shem Johnson, Jean Gruenberg -- Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking / Jean-Baptiste Manneville, Cécile Leduc, Benoit Sorre, Guillaume Drin -- Reconstituting multivesicular body biogenesis with purified components / Thomas Wollert -- Approaches to the study of Atg8-mediated membrane dynamics in vitro / Anjai Jotwani, Diana N. Richerson, Isabelle Motta, Omar Julca-Zevallos, Thomas J. Melia -- Reconstitution assay system for ceramide transport with semi-intact cells / Keigo Kumagai, Masahiro Nishijima, Kentaro Hanada -- Visualizing mitochondrial lipids and fusion events in mammalian cells / Huiyan Huang, Michael A. Frohman -- Targeted and non-targeted analysis of membrane lipids using mass spectrometry / Xue Li Guan, Markus R. Wenk -- Modulation of host phosphoinositide metabolism during salmonella invasion by the type III secreted effector SopB / Bernhard Roppenser, Sergio Grinstein, John H. Brumell -- Acute manipulation of phosphoinositide levels in cells / Belle Chang-Ileto, Samual G. Frere, Gilbert Di Paolo -- Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins / Marnix Wieffer, Volker Haucke, Michael Krauß -- Phosphoinositides at the neuromuscular junction of drosophila melanogaster : a genetic approach / Jan R. Slabbaert, Thang Manh Khuong, Patrik Verstreken -- Devising powerful genetics, biochemical and structural tools in the functional analysis of phosphatidylinositol transfer proteins (PITPs) across diverse species / James M. Davison, Vytas A. Bankaitis, Ratna Ghosh -- Genome-wide screens for gene products regulating lipid droplet dynamics / Weihua Fei, Hongyuan Yang -- The three dimensionality of cell membranes : lamellar to cubic membrane transition as investigated by electron microsocopy / Ketpin Chong, Yuru Deng -- Quantitative imaging of lipid metabolism in yeast : from 4D analysis to high content screens of mutant libraries / Heimo Wolinski, Kristian Bredies, Sepp D. Kohlwein -- Analysis of cholesterol trafficking with fluorescent probes / Frederick R. Maxfield, Daniel Wüstner -- Fluorescence correlation methods for imaging cellular behavior of sphingolipid-interacting probes / Rachel Kraut, Nirmalya Bag, Thorsten Wohland -- Monitoring phospholipid dynamics during phagocytosis : application of genetically-encoded fluorescent probes / Helen Sarantis, Sergio Grinstein -- Genetically encoded probes for phosopatidic acid / Nawal Kassas, Petra Tryoen-Tóth, Matthias Corrotte, Tamou Thahouly, Marie-France Bader, Nancy J. Grant, Nicolas Vitale.
  • 2005 Springer
    edited by Anne Kathrine Hvoslef-Eide and Walter Preil.
  • 2012 Springer Protocols
    edited by Takahiro Ochiya.
    Purification and culture of fetal mouse hepatoblasts that are precursors of mature hepatocytes and biliary epithelial cells / Nobuyoshi Shiojiri and Miho Nitou -- Clinical uses of liver stem cells Clinical uses of liver stem cells / Yock Young Dan -- Identification and isolation of adult liver stem/progenitor cells / Minoru Tanaka and Atsushi Miyajima -- Isolation and purification method of mouse fetal hepatoblasts / Luc Gailhouste -- Isolation of hepatic progenitor cells from the galactosamine-treated rat liver / Norihisa Ichinohe, Junko Kon, and Toshihiro Mitaka -- Purification of adipose tissue mesenchymal stem cells and differentiation toward hepatic-like cells / Agnieszka Banas -- Development of immortalized hepatocyte-like cells from hMSCs / Adisak Wongkajornsilp, Khanit Sa-ngiamsuntorn, and Suradej Hongeng -- Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages / Shohei Wakao [and others] -- Generation and hepatic differentiation of human iPS cells / Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya -- Efficient hepatic differentiation from human iPS cells by gene transfer / Kenji Kawabata, Mitsuru Inamura, and Hiroyuki Mizuguchi -- "Tet-on" system toward hepatic differentiation of human mesenchymal stem cells by hepatocyte nuclear factor / Goshi Shiota and Yoko Yoshida -- SAMe and HuR in liver physiology / Laura Gomez-Santos [and others] -- Transdifferentiation of mature hepatocytes into bile duct/ductule cells within a collagen gel matrix / Yuji Nishikawa -- Identification of cancer stem cell-related microRNAs in hepatocellular carcinoma / Junfang Ji and Xin Wei Wang -- Intravenous human mesenchymal stem cells transplantation in NOD/SCID mice preserve liver integrity of irradiation damage / Moubarak Mouiseddine [and others] -- Engineering of implantable liver tissues / Yasuyuki Sakai [and others] -- Mesenchymal stem cell therapy on murine model of nonalcoholic steatohepatitis / Yoshio Sakai and Shuichi Kaneko.
  • 2006 Springer
    edited by Constantine Tsoukas.
    Protein kinase C-theta (PKC[theta]): new perspectives on its functions in T-cell biology / Rina Barouch-Bentov and Amnon Altman -- Travel informations on the tec kinases during lymphocyte activation / Fabien Garçon and Jacques A. Nunès -- Inducible T-cell tyrosine kinase (ITK): structural requirements and actin polymerization / Constantine D. Tsoukas ... [et al.] -- Differential requirements of PI3K subunits for BCR or BCR/CD19-induced ERK activation / Elena Vigorito and Martin Turner -- Protein tyrosine phosphatases in human disease / Tomas Mustelin -- The T-cell-specific adapter protein functions as a regulator of peripheral but not central immunological tolerance / Philip E. Lapinski ... [et al.] -- Proximal signals controlling B-cell antigen receptor (BCR) mediated NF-[kappa]B activation / Miguel E. Moreno-García ... [et al.] -- The adapter 3BP2: how it plugs into leukocyte signaling / Marcel Deckert and Robert Rottapel -- CTLA-4 regulation of T-cell function via RAP-1-mediated adhesion / Helga Schneider ... [et al.] -- Protein crosstalk in lipid rafts / Raquel J. Nunes, Mónica A.A. Castro, and Alexandre M. Carmo -- Role of lipid rafts in activation-induced cell death: the fas pathway in aging / Anis Larbi ... [et al.] -- T-cell response in aging: influence of cellular cholesterol modulation / Tamàs Fülöp ... [et al.] -- Lymphocyte signaling and the translatability of mRNA / Suzanne Miyamoto -- Protein arginine methylation: a new frontier in T-cell signal transduction / Brandon T. Schurter, Fabien Blanchet, and Oreste Acuto -- Immune regulation by ubiquitin conjugation / K. Venuprasad ... [et al.] -- CTIB (C-terminus protein of I[kappa]B-[beta]): a novel factor required for acidic adaptation /Toshiko Fukamachi ... [et al.] -- HIV may deplete most CD4 lymphocytes by a mechanism involving signaling through its receptors on non-permissive resting lymphocytes / Miles W. Cloyd ... [et al.] -- Integrating traditional and postgenomic approaches to investigate lymphocyte development and function / Yina Hsing Huang ... [et al.] -- Phosphoproteomic analysis of lymphocyte signaling / Lulu Cao, Kebing Yu, and Arthur R. Salomon.
    Also available: Print – 2006
  • 2005 Springer
    Paul Saftig.
    History and morphology of the lysosome / Renate Lüllmann-Rauch -- Transport of lysosomal enzymes / Stephan Storch and Thomas Braulke -- Adaptor proteins in lysosomal biogenesis / Peter Schu -- Lysosomal membrane proteins / Paul Saftig -- Lysosomal proteases: revival of the sleeping beauty / Klaudia Brix -- Lysosomal storage disorders / Ole Kristian Greiner-Tollersrud and Thomas Berg -- Lysosomal transporters and associated diseases / Frans W. Verheijen and Grazia M.S. Mancini -- Neuronal ceroid-lipofuscinoses / Jaana Tyynelè -- Cholesterol transport in lysosomes / Judith Storch and Sunita R. Cheruku -- Therapy of lysosomal storage diseases / Ulrich Matzner -- Lysosomal proteome and transcriptome / Jobst Landgrebe and Torben Lubke -- External lysosomes: the osteoclast and its unique capacities / Vincent Everts and Wouter Beertsen -- Membrane resealing mediated by lysosomal exocytosis / Norma W. Andrews -- Macroautophagy in mammalian cells / Eeva-Liisa Eskelinen -- Chaperone-mediated autophagy / Erwin Knecht and Natalia Salvador.
  • 2015 ScienceDirect
    edited by Frances Platt, Nick Platt.
  • 2009 Springer
    David S. Goodsell.
    Introduction -- Molecular machines -- The processes of living -- Molecules in cells: Escherichia coli -- A human cell: The advantages of compartments -- The human body: The advantages of specialization -- Life and death -- Viruses -- You and your molecules.
  • 2009 Springer Protocols
    edited by Neil E. Reiner.
    Generation of retroviral macrophage cDNA expression libraries and functional screening for surface receptors / Georgia Schäfer and Gordon D. Brown -- Generation of murine growth factor-dependent long-term dendritic cell lines to investigate host-parasite interactions / Alessandra Mortellaro ... [et al.] -- Macrophage activation : classical vs. alternative / Andrea Classen, Jorge Lloberas, and Antonio Celada -- Analysis of macrophage phagocytosis : quantitative assays of phagosome formation and maturation using high-throughput florescence microscopy / Benjamin E. Steinberg and Sergio Grinstein -- Detection of activated Rab7 GTPase with an immobilized RILP probe / Jim Sun ... [et al.] -- Probing in vivo origins of mononuclear phagocytes by conditional ablation and reconstitution / Chen Varol, Limor Landsman, and Steffen Jung -- Multivalent protein probes for the identification and characterization of cognate cellular ligands for myeloid cell surface receptors / Hsi-Hsien Lin ... [et al.] -- Exploiting Fc chimaeric proteins for the identification of ligands specific for the mannose receptor / Luisa Martinez-Pomares -- The expression of exogenous genes in macrophages : obstacles and opportunities / Xia Zhang, Justin P. Edwards, and David M. Mosser -- Genetic modification of murine dendritic cells by RNA transfection / Maurizio Ceppi, Enrico Schmidt, and Philippe Pierre -- Recording phagosome maturation through the real-time, spectrofluorometric measurement of hydrolytic activities / Robin M. Yates, Albin Hermetter, and David G. Russell -- Derivation and characterization of murine alternatively activated (M2) macrophages / Victor W.H. Ho and Laura M. Sly -- Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology / Mattias Svensson -- Generation, culture, and flow-cytometric characterization of primary mouse macrophages / Ulrike Schleicher and Christian Bogdan -- Analyzing classical and alternative macrophage activation in macrophage/neutrophil-specific IL-4 receptor-alpha-deficient mice / Frank Brombacher ... [et al.] -- Analysis of matrix metalloproteinase secretion by macrophages / Paul T. Elkington, Justin A. Green, and Jon S. Friedland -- Identification of pathogen receptors on dendritic cells to understand their function and to identify new drug targets / Lot de Witte ... [et al.] -- Stable lentiviral vector-mediated gene silencing in human monocytic cell lines / Jimmy S. Lee and Neil E. Reiner -- Use of fluorescent probes to detect lipid signaling intermediates in macrophages / Vivan Kwok, Eric Vachon, and Gregory P. Downey -- Large-scale phagosome preparation / Adrien F. Vinet and Albert Descoteaux -- Coordinate regulation of sphingosine kinase and actin dynamics / Shankar S. Iyer and David J. Kusner.
  • 2008 ScienceDirect
    edited by M. Zborowski, J.J. Chalmers.
    Also available: Print – 2008
  • 2011 Springer
    Kyle E. Orwig, Brian P. Hermann, editors.
    Scientists investigating germ cells have, over the past 15 years, originated discoveries and innovations that give us valuable insights into the mechanisms that regulate not just stem cell function, but human development in its widest sense. With contributions from some of the leading researchers in the field, Male Germline Stem Cells: Developmental and Regenerative Potential assesses the implications of these discoveries for understanding the fundamental biology of germline stem cells as well as their potential for human stem cell-based therapies. This monograph covers many of the fundamental issues now being explored by today's generation of stem cell researchers, including the field's potential for regenerative medicine.
  • 2013 Springer Protocols
    edited by Hayden A. Homer.
    Studying recombination in mouse oocytes / Xianfei Sun and Paula E. Cohen -- Analysis of recombinants in female mouse meiosis / Esther de Boer, Maria Jasin, and Scott Keeney -- Studying meiosis-specific cohesins in mouse embryonic oocytes / Ji-hye Kim [and others] -- Immunohistochemical approaches to the study of human fetal ovarian development / Jing He [and others] -- Protein kinase assays for measuring MPF and MAPK activities in mouse and rat oocytes and early embryos / Jacek Z. Kubiak -- Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis I in mouse oocytes / Janet E. Holt, Simon I.R. Lane, and Keith T. Jones -- Using FRET to study ranGTP gradients in live mouse oocytes / Julien Dumont and Marie-Hélène Verlhac -- Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes / Mark Levasseur -- RNAi-based methods for gene silencing in mouse oocytes / Paula Stein, Petr Svoboda, and Richard M. Schultz -- Micro-injection of morpholino oligonucleotides for depleting securin in mouse oocytes / Petros Marangos -- Measuring transport and accumulation of radiolabeled substrates in oocytes and embryos / Jay M. Baltz, Hannah E. Corbett, and Samantha Richard -- Immunofluorescence staining of spindles, chromosomes, and kinetochores in human oocytes / Solon Riris [and others] -- Studying the roles of aurora-C kinase during meiosis in mouse oocytes / Kuo-Tai Yang [and others] -- Chromosome spreads with centromere staining in mouse oocytes / Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann -- Preparation of mammalian oocytes for transmission electron microscopy / Lynne Anguish and Scott Coonrod -- Measuring Ca² oscillations in mammalian eggs / Karl Swann -- Counting chromosomes in intact eggs / Teresa Chiang and Michael A. Lampson -- Free-hand bisection of mouse oocytes and embryos / Zbigniew Polanski and Jacek Z. Kubiak -- Microarray-CGH for the assessment of aneuploidy in human polar bodies and oocytes / Souraya Jaroudi and Dagan Wells -- Nuclear transfer in the mouse oocyte / Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama.
  • 2013 Cambridge
    Joseph H. Antin, Harvard Medical School and Dana-Farber Brigham and Women's Cancer Center, Boston, MA, Deborah Yolin Raley, Dana-Farber Brigham and Women's Cancer Center, Boston, MA.
    1. Rationale for transplantation -- 2. Types of transplantation -- 3. Human leukocyte antigen matching in allogeneic transplantation -- 4. Stem cell source -- 5. Pre-transplant evaluation and counseling of patient and donor -- 6. Conditioning regimens -- 7. Stem cell infusion -- 8. ABO compatibility -- 9. Engraftment -- 10. Preventative care -- 11. Transplant-related complications -- 12. Overview of acute and chronic graft-versus-host disease -- 13. Acute graft-versus-host disease and staging -- 14. Graft-versus-host prophylactic regimens -- 15. Treatment guidelines for acute graft-versus-host disease -- 16. Chronic graft-versus-host disease -- 17. Engraftment syndrome -- 18. Infectious disease -- 19. Graft rejection and failure -- 20. Gastrointestinal complications -- 21. Oral health in stem cell transplantation -- 22. Pulmonary complications -- 23. Veno-occlusive disease -- 24. Special transfusion-related situations -- 25. Cardiovascular complications -- 26. Neurologic complications -- 27. Cystitis -- 28. Donor lymphocyte infusion -- 29. Transplantation: regulation and accreditation -- Index.
  • 2011 Springer
    edited by Alasdair M. Gilfillan [and] Dean D. Metcalfe.
    Mast cell biology : introduction and overview / Alasdair M. Gilfillan, Sarah J. Austin, and Dean D. Metcalfe -- Mast cell progenitor trafficking and maturation / Jenny Hallgren and Michael F. Gurish -- Monomeric IgE and mast cell development, survival, and function / Jun-ichi Kashiwakura, Iris M. Otani, and Toshiaki Kawakami -- Mast cell apoptosis and survival / Maria Ekoff and Gunnar Nilsson -- Regulators of Ca2+ signaling in mast cells: Potential targets for treatment of mast cell-related diseases / Hong-Tao Ma and Michael A. Beaven -- Spatio-temporal signaling in mast cells / Bridget S. Wilson, Janet M. Oliver, and Diane S. Lidke -- The mechanisms of exocytosis in mast cells / Ulrich Blank -- An emerging role for the lipid mediator sphingosine-1-phosphate in mast cell effector function and allergic disease / Ana Olivera and Juan Rivera -- Down-regulation of mast cell responses through itim containing inhibitory receptors / Laila Karra and Francesca Levi-Schaffer -- The mast cell in innate and adaptive immunity / Christopher P. Shelburne and Soman N. Abraham -- Mast cells and immunoregulation/immunomodulation / Mindy Tsai, Michele Grimbaldeston, and Stephen J. Galli -- Mast cell proteases as protective and inflammatory mediators / George H. Caughey -- Mast cells in lung inflammation / Elena P. Moiseeva and Peter Bradding -- Mast cells, angiogenesis, and cancer / Domenico Ribatti and Enrico Crivellato.
    Also available: Print – 2011
  • 2015 Springer Protocols
    edited by Michael R. Hughes and Kelly M. McNagny.
    Paul Ehrlich's mastzellen : a historical perspective of relevant developments in mast cell biology / Jack Ghably [and others] -- Phylogenetic profile of mast cells / Enrico Crivellato, Luciana Travan, and Domenico Ribatti -- Mast cell development and function in the Zebrafish / Sahar I Da'as, Tugce B. Balci, and Jason N. Berman -- Human mast cell and basophil/eosinophil progenitors / Gail M. Gauvreau and Judah A. Denburg -- Methods for the study of mast cell recruitment and accumulation in different tissues / Tatiana G. Jones and Michael F. Gurish -- Notch2 signaling in mast cell development and distribution in the intestine / Mamiko Sakata-Yanagimoto and Shigeru Chiba -- Mast cells in human health and disease / Erin J. DeBruin ... [e tal.] -- Emerging prominence of the cardiac mast cell as a potent mediator of adverse myocardial remodeling / Joseph S. Janicki, Gregory L. Brower, and Scott P. Levick -- Parasympathetic nervous system as a regulator of mast cell function / Paul Forsythe -- Growth of human mast cells from bone marrow and peripheral blood-derived CD34+ pluripotent hematopoietic cells / Geethani Bandara, Dean D. Metcalfe, and Arnold S. Kirshenbaum -- Isolation and characterization of human intestinal mast cells / Axel Lorentz, Gernot Sellge, and Stephan C. Bischoff -- Human mast cell activation with viruses and pathogen products / Ian D. Haidl and Jean S. Marshall -- Basic techniques to study Fc[epsilon]RI signaling in mast cells / Yuko Kawakami and Toshiaki Kawakami -- Membrane-cytoskeleton dynamics in the course of mast cell activation / Pavel Dráber and Petr Dráber -- Fc[epsilon]RI expression and dynamics on mast cells / Eon J. Rios and Janet Kalesnikoff -- Regulation of mast cell survival and apoptosis / Christine Möller Westerberg, Maria Ekoff, and Gunnar Nilsson -- Protein tyrosine phosphatases in mast cell signaling / Alexander Geldman and Catherine J. Pallen -- MicroRNA function in mast cell biology : protocols to characterize and modulate MicroRNA expression / Steven Maltby [and others] -- Assay of mast cell mediators / Madeleine Rådinger [and others] -- Induction of mast cell apoptosis by a novel secretory granule-mediated pathway / Fabio R. Melo, Sara Wernersson, and Gunnar Pejler -- Measurement of nitric oxide in mast cells with the fluorescent indicator DAF-FM diacetate / Chris D. St. Laurent, Tae Chul Moon, and A. Dean Befus -- Real-time imaging of Ca2+ mobilization and degranulation in mast cells / Roy Cohen, David A. Holowka, and Barbara A. Baird -- Flow cytometry-based monitoring of mast cell activation / Glenn Cruse, Alasdair M. Gilfillan, and Daniel Smrz -- Measurement of mast cell surface molecules by high- throughput immunophenotyping using transcription (HIT) / D. James Haddon [and others] -- Cre/loxP-based mouse models of mast cell deficiency and mast cell-specific gene inactivation / Katrin Peschke [and others] -- Evaluation of synovial mast cell functions in autoimmune arthritis / Peter A. Nigrovic and Kichul Shin -- Methods for the study of mast cells in cancer / Nichole R. Blatner, FuNien Tsai, and Khashayarsha Khazaie -- Studying mast cells in peripheral tolerance by using a skin transplantation model / Victor C. de Vries [and others] -- Function of mast cells in autoimmune glomerulonephritis / Renato C. Monteiro [and others] -- Mouse model of atopic dermatitis / Yuko Kawakami and Toshiaki Kawakami -- Mouse models of allergic asthma / Matthew Gold, David Marsolais, and Marie-Renee Blanchet -- Methods in assessment of airway reactivity in mice / Matthew Gold and Marie-Renee Blanchet.
  • 2011 Springer
    Carmen Molina-París, Grant Lythe, editors.
    Preface -- Thymocyte development -- A review of mathematical models for T cell receptor triggering and antigen discrimination -- Dynamic tuning of T cell receptor specificity by co-receptors and costimulation -- T cell activation and function: role of signal strength -- The cyton model for lymphocyte proliferation and differentiation -- Modeling itravital two-photon data of lymphocyte migration and interaction -- Modeling lymphocyte dynamics in vivo -- Continuous-time birth and death processes: diversity maintenance of naïve T cells in the periphery -- Multivariate competition processes: a model for two competing T cell clonotypes -- Stochastic modeling of T Cell homeostasis for two competing clonotypes via the master equation -- Dendritic cell migration in the intestinal tract -- Reassessing germinal center reaction concepts -- B cell strategies of Ag recognition in a stratified immune system -- Dynamics of Peripheral regulatory and effector T cells competing for antigen presenting cells -- Mathematical models of the role of IL-2 in the interactions between helper and regulstory CD4+ T cells -- A Physicist{u2019}s approach to immunology -- Timescales of the adaptive immune response -- Using mathematical models to explore the role of cytoxic T lymphocytes in HIV infection -- Viral immunity and persistence -- Index.
  • 2009 Springer
    Andre Kamkin, Ilya Lozinsky, editors ; foreword by Maik Gollasch.
    The volume dwells on the major issues of mechanical stress influencing the ion channels and intracellular signaling pathways. This book is a unique collection of reviews outlining current knowledge and future developments in this rapidly growing field. In our opinion the book presents not only the latest achievements in the field but also brings the problem closer to the experts in related medical and biological sciences as well as practicing doctors. Knowledge of the mechanisms which underlie these processes is necessary for understanding of the normal functioning of different living organs and tissues and allows to predict changes, which arise due to alterations of their environment, and possibly will allow to develop new methods of artificial intervention. We also hope that presenting the problem will attract more attention to it both from researchers and practitioners and will assist to efficiently introduce it into the practical medicine.
  • 2005 NCBI Bookshelf
    edited by Andre Kamkin and Irina Kiseleva.
    Mechanosensitivity of cells from various tissues -- Mechanotransduction in the Nematode Caenorhabditis elegans -- Mechanically mediated crosstalk in heart -- Swelling-and stretch-activated chloride channels in the heart: regulation and function -- Role of stretch-activated channels in the heart: action potential and Ca2+ transients -- Isolated cardiomyocytes: mechanosensitivity of action potential, membrane current and ion concentration -- Mechano-electric feedback in the heart: evidence from intracellular microelectrode recordings on multicellular preparations and single cells from healthy and diseased tissue -- The Role of mechanosensitive fibroblasts in the heart -- Mechanical modulation of intracellular ion concentrations: mechanisms and electrical consequences -- Stretch-induced slow force response in mammalian ventricular myocardium -- The Na+/H+ exchanger as the main protagonist following myocardial stretch: the Anrep effect and myocardial hypertrophy -- Mechano-electric feedback and atrial arrhythmias -- The Role of mechano-electrical feedback in the cholinergic atrial fibrillation initiation --; Mechanosensitive cation channels of leech neurons -- Mechanosensitivity of primary afferent nociceptors in the pain pathway -- Stimulus-secretion coupling in the osmoreceptive prolactin cell of the tilapia -- Stretch-inactivated channels in skeletal muscle -- Stretch-activated cation channels and the myogenic response of small arteries -- Mechanobiology of bone tissue and bone cells -- Functional roles of mechanosensitive ion channels, ss1 integrins and kinase cascades in chondrocyte mechanotransduction -- Enigmatic roles of the epithelial sodium channel (ENaC) in articular chondrocytes and osteoblasts: mechanotransduction, sodium transport or extracellular sodium sensing?
  • v. 1-, 2008- Springer
  • 2008
    edited by Steven R. Goodman.
    Status: Not Checked OutLane Catalog Record
  • 2012
    [edited by] Walter F. Boron, MD, PhD, professor, David N. and Inez Myers/Antonio Scarpa Chairman, Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio, Emile L. Boulpaep, MD, professor, Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut.
    Status: Not Checked OutLane Catalog Record
    Medical Physiology, in its updated 2nd edition, firmly relates molecular and cellular biology to the study of human physiology and disease. Drs. Walter Boron and Emile Boulpaep and a team of leading physiologists present practical, accurate coverage emphasizing the clinical implications of the material. Each chapter explains the principles and organization of each body system, while more than 1400 high-quality, full-color line drawings and prominently featured clinical examples clarify every concept. This exceptionally detailed and comprehensive guide to physiology is ideal for a rich, straightforward, state-of-the-art understanding of this essential subject.
  • 2017 ClinicalKey
    [edited by] Walter F. Boron, Emile L. Boulpaep.
  • 2013 Springer Protocols
    edited by Doron Rapaport, Johannes M. Herrmann.
    Quantitative analysis of cellular lipids by nano- electrospray ionization mass spectrometry / Cagakan Özbalci, Timo Sachsenheimer, and Britta Brügger -- Thin-layer chromatography of phospholipids / Rania M. Deranieh, Amit S. Joshi, and Miriam L. Greenberg -- Analysis of membrane lipid biogenesis pathways using yeast genetics / Martina Gsell and Günther Daum -- Using 2D crystals to analyze the structure of membrane proteins / Ian Collinson, Janet Vonck, and Dilem Hizlan -- Crystallization of membrane proteins / Florian G. Müller and C. Roy D. Lancaster -- Molecular dynamics simulations of membrane proteins / Kristyna Pluhackova, Tsjerk A. Wassenaar, and Rainer A. Böckmann -- Site-specific fluorescent probe labeling of mitochondrial membrane proteins / Christine T. Schwall and Nathan N. Alder -- Topology determination of untagged membrane proteins / Iris Nasie, Sonia Steiner-Mordoch, and Shimon Schuldiner -- self-assembling GFP : a versatile tool for plant (membrane) protein analyses / Katharina Wiesemann ... [et al.] -- Use of cardiolipin-containing liposomes as a model system to study the interaction between proteins and the inner mitochondrial membrane / Milit Marom and Abdussalam Azem -- Analysis of the interaction between membrane proteins and soluble binding partners by surface plasmon resonance / Zht Cheng Wu... [et al.] -- Peptide interaction with and insertion into membranes / Ron Saar-Dover, Avraham Ashkenazi, and Yechiel Shai -- Scanning fluorescence correlation spectroscopy in model membrane systems / Joseph D. Unsay and Ana J. García-Sáez -- Analyses of protein-protein Interactions by in vivo photocrosslinking in budding yeast / Takuya Shiota, Shuh-ichi Nishikawa, and Toshiya Endo -- Sedimentation velocity analytical ultracentrifugation in hydrogenated and deuterated solvents for the characterization of membrane proteins / Aline Le Roy ... [et al.] -- Membrane partitioning and translocation studied by isothermal titration calorimetry / Carolyn Vargas, Johannes Klingler, and Sandro Keller -- Analyzing membrane dynamics with live cell fluorescence microscopy with a focus on yeast mitochondria / Dirk Scholz ... [et al.] -- Analysis of protein translocation into the endoplasmic reticulum of human cells / Johanna Dudek ... [et al.] -- Assay to monitor the membrane integration of single-span proteins / Katrin Krumpe and Doron Rapaport -- Methods to study the biogenesis of membrane proteins in yeast mitochondria / Daniel Weckbecker and Johannes M. Herrmann -- Reconstitution of mitochondrial presequence translocase into proteoliposomes / Martin van der Laan, Ralf M. Zerbes, and Chris van der Does -- Single channel analysis of membrane proteins in artificial bilayer membranes / Philipp Bartsch, Anke Harsman, and Richard Wagner -- Quantification of protein complexes by blue native electrophoresis / Juliana Heidler ... [et al.] -- Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions / Anna Hjelm ... [et al.] .
  • 2005 Springer
    edited by Mark P. Mattson.
    From intramolecular asymmetries to raft assemblies: a short guide for the puzzled in lipidomics / Ephraim Yavin and Annette Brand -- Regulatory aspects of membrane microdomain (raft) dynamics in live cells: a biophysical approach / János Matkó and János Szöllosi -- Lipid raft membrane skeletons / Elizabeth J. Luna ... [et al.] -- Role of cholesterol in membrane microdomain signaling / Christopher J. Fielding -- Raft lipid metabolism in relation to alkyl-lysophospholipid-induced apoptosis / Arnold H. van der Luit, Marcel Verheij, and Wim J. van Blitterswijk -- Role of lipid rafts in signal transduction and synaptic plasticity of neural cells / Markus Delling and Melitta Schachner -- Role of rafts in virus fusion and budding / Wu Ou and Jonathan Silver -- Alterations in raft lipid metabolism in aging and neurodegenerative disorders / Mark P. Mattson, Roy G. Cutler, and Norman J. Haughey -- Caveolin and cancer: a complex relationship / Mordechai Liscovitch ... [et al.] -- Dietary modulation of lipid rafts: implications for disease prevention and treatment / Mark P. Mattson.
  • 2015 Springer Protocols
    edited by Bor Luen Tang.
    This second edition explores membrane trafficking research that has been at the frontier of cell and molecular biology. In the past 20 years, this field has witnessed a remarkable convergence and synergism from information gained using genetic approaches in yeast cells, and biochemical and molecular approaches in mammalian cells. This volume contains updated chapters and the inclusion of a large number of new chapters, and is divided into three parts. Part I includes biochemical and molecular genetics approaches and methods used in analysing membrane traffic, in both yeast and mammalian cell models. Part II focuses on imaging and microscopy approaches and techniques, while Part III highlights the Omics-type approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and resourceful, Membrane Trafficking, Second Edition, is a valuable resource for both experienced researchers, graduate students, and junior scientists in the field of membrane trafficking.
  • 2008 Springer Protocols
    edited by Ales Vancura.
    Pt. I. Yeast Cells -- Ch. 1. Freight management in the cell / Johannes M. Herrmann, Anne Spang -- Ch. 2. High-throughput protein extraction and immunoblotting analysis in saccharomyces cerevisiae / Todd C. Lorenz, Vikram C. Anand, Gregory S. Payne -- Ch. 3. Genome-wide analysis of membrane transport using yeast knockout arrays / Helen E. Burston, Michael Davey, Elizabeth Conibear -- Ch. 4. A cell-free system for reconstitution of transport between prevacuolar compartments and vacuoles in saccharomyces cerevisiae / Thomas A. Vida -- Ch. 5. In vitro analysis of the mitochondrial preprotein import machinery using recombinant precursor polypeptides / Dorothea Becker, Martin Krayl, Wolfgang Voos -- Ch. 6. In vitro import of proteins into isolated mitochondria / Karl Bihlmaier, Melanie Bien, Johannes M. Herrmann -- Ch. 7. Synthesis and sorting of mitochondrial translation products / Heike Bauerschmitt, Soledad Funes, Johannes M. Herrmann -- Ch. 8. In vivo labeling and analysis of mitochondrial translation products in budding and in fission yeasts / Karine Gouget, Fulvia Verde, Antoni Barrientos -- Ch. 9. Exploring protein-protein interactions involving newly synthesized mitochondrial DNA encoded proteins / Darryl Horn, Flavia Fontanesi, Antoni Barrientos -- Ch. 10. Purification of yeast membranes and organelles by sucrose density gradient centrifugation / Jennifer Chang, Victoria Ruiz, Ales Vancura -- Ch. 11. Microscopic analysis of lipid droplet metabolism and dynamics in yeast / Heimo Wolinski, Sepp D. Kohlwein -- Ch. 12. Use of bimolecular fluorescence complementation (BiFC) in yeast saccharomyces cerevisiae / Kari-Pekka Skarp, ... [et al.] -- Pt. II. Mammalian cells -- Ch. 13. Using quantitative fluorescence microscopy to probe organelle assembly and membrane trafficking / Brian Storrie, Tregei Starr, Kimberly Forsten-Williams -- Ch. 14. Measuring secretory membrane traffic / Vytaute Starkuviene, ... [et al.] -- Ch. 15. A correlative light and electron microscopy method based on laser micropatterning and etching / Julien Colombelli, ... [et al.] -- Ch. 16. Approaches to investigate the role of signaling in ER-to-Golgi transport / Laura J. Sharpe, Ximing Du, Andrew J. Brown -- Ch. 17. Recruitment of coat proteins to peptidoliposomes / Gregor Suri, Martin Spiess, Pascal Crottet -- Ch. 18. SNAREs mediated fusion of liposomes / Jérôme Vicogne, Jeffrey E. Pessin -- Ch. 19. Use of polarized PC12 cells to monitor protein localization in the early biosynthetic pathway / Ragna Sannerud, ... [et al.] -- Ch. 20. Tracking the transport of E-cadherin to and from the plasma membrane / Matthew P. Wagoner, Kun Ling, Richard A. Anderson -- Ch. 21. Analysis of nucleocytoplasmic shuttling of NFkB proteins in human leukocytes / Chandra C. Ghosh ... [et al.] -- Ch. 22. Imaging pHluorin-based probes at hippocampal synapses / Stephen J. Royle, ... [et al.] -- Ch. 23. Analysis of receptor tyrosine kinase internalization using flow cytometry / Ning Li, Kristen S. Hill, Lisa A. Elferink -- Ch. 24. Measurement of receptor endocytosis and recycling / Jane M. Knisely, Jiyeon Lee, Guojun Bu -- Ch. 25. Styryl dye-based synaptic vesicle recycling assay in cultured cerebellar granule neurons / Victor Anggono, Michael A. Cousin, Phillip J. Robinson -- Ch. 26. Use of quantitative immunofluorescence microscopy to study intracellular trafficking / Vincent Blot, Timothy E. McGraw -- Ch. 27. Dissecting GLUT4 traffic components in L6 myocytes by fluorescence-based, single cell assays / Costin N. Antonescu, Varinder K Randhawa, Amira Klip -- Ch. 28. Functional genetic analysis of the mammalian mitochondrial DNA encoded peptides / Maria Pilar Bayona-Bafaluy, ... [et al.].
  • 2015 ScienceDirect
    edited by Yaoliang Tang, Buddhadeb Dawn.
    Insights into the Mechanism of Exosome Formation and Secretion / Kobina Essandoh, Guo-Chang Fan -- An Overview of the Proteomic and miRNA Cargo in MSC-Derived Exosomes / Soon Sim Tan, Tian Sheng Chen, Kok Hian Tan, Sai Kiang Lim -- Exosome Function in miRNA-Mediated Paracrine Effects / Sathyamoorthy Balasubramanian, Sheeja Rajasingh, Jayakumar Thangavel, Buddhadeb Dawn, Johnson Rajasingh -- Current Methods to Purify and Characterize Exosomes / W. Michael Dismuke, Yutao Liu -- Stem Cell Extracellular Vesicles: A Novel Cell-Based Therapy for Cardiovascular Diseases / Ewa K. Zuba-Surma, Marta Adamiak, Buddhadeb Dawn -- Therapeutic Potential of Stem Cell-Derived Extracellular Vesicles in Cardioprotection and Myocardium Repair / Bin Yu, Muhammad Ashraf, Meifeng Xu -- Engineered/Hypoxia-Preconditioned MSC-Derived Exosome: Its Potential Therapeutic Applications / Wei Zhu, Han Chen, Jian'an Wang -- Exosome-Based Translational Nanomedicine: The Therapeutic Potential for Drug Delivery / Lei Lv, Qingtan Zeng, Shenjun Wu, Hui Xie, Jiaquan Chen, Xiang Jiang Guo, Changning Hao, Xue Zhang, Meng Ye, Lan Zhang -- Effect of Exosomes from Mesenchymal Stem Cells on Angiogenesis / Susmita Sahoo, Feng Dong, Lola DiVincenzo, William Chilian, Liya Yin -- Exosomes for Bone Diseases / Paulomi Sanghavi, Porter Young, Sunil Upadhyay, Mark W. Hamrick -- Diagnostic and Prognostic Applications of MicroRNA-Abundant Circulating Exosomes / Baron Arnone, Xiaoqi Zhao, Zhipeng Zou, Gangjian Qin, Min Cheng.
  • 2013 Springer
    Lucas G. Chase, Mohan C. Vemuri, editors.
    Mesenchymal Stem Cells or Multipotent Mesenchymal Stromal Cells (collectively referred to as MSCs) are currently being tested in a number of clinical trials for various diseases including graft-versus-host disease, Crohn's disease, myocardial infarction, stroke, diabetes, cartilage defects, and many others. The increasing number of clinical trials globally indicates the likely therapeutic potential of this specialized adult stem cell. Over the last decade, there has been tremendous progress towards understanding an MSC's phenotype, expansion features in culture, differentiation ability and global gene and protein expression profiles. Despite the enormous progress and data accrual at the molecular and cellular levels, several questions remain as to the utility of MSCs for clinical applications. Mesenchymal Stem Cell Therapy is a comprehensive collection of information comprised of both basic and pre-clinical biological studies as well as current advances in clinical applications within the MSC therapy field. This text is a resource for both the basic life science and cell therapy researchers and includes a spectrum of review chapters from top experts in the field discussing clinical scale culture, regulatory issues, genetic engineering, disease specific applications and others.
  • 2008 Springer Protocols
    edited by Darwin J. Prockop, Donald G. Phinney, Bruce A. Bunnell.
    Part I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.]. Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger. A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C. W. Zannettino. Adipose-derived stem cells / John K. Fraser ... [et al.]. Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois ... [et al.] -- Part II. Manipulation of human multipotential stromal cells. Colony forming unit assays for MSCs / Radhika Pochampally. Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe. Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe. Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi. Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prockop. Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- Part III. Isolation and characterization of murine multipotential stromal cells from bone marrow. Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney.
  • 2016 Springer
    Piotr Dziegiel, Bartosz Pula, Christopher Kobierzycki, Mariusz Stasiolek, Marzenna Podhorska-Okolow.
    Introduction -- Metallothioneins : structure and functions -- Metallothionein-3 -- The role of metallothioneins in carcinogenesis -- Metallothioneins and immune function.
  • v.1-2, 2012-2014 Springer Protocols
    v.2 (2014) Springer Protocols
    edited by Miriam Dwek, Susan A. Brooks, Udo Schumacher.
  • 2013 ScienceDirect
    edited by Franck Perez and David J. Stephens.
  • 2002 ScienceDirect
    prepared under the auspices of the American Society for Cell Biology ; edited by Josephine C. Adams.
    Also available: Print – 2002
  • 2015 ScienceDirect
    edited by Renata Basto and Wallace F. Marshall.
    Imaging centrosomes and cilia in the mouse kidney / Masato Hoshi, Jinzhi Wang, Sanjay Jain, Moe R. Mahjoub -- Ependymal cell differentiation, from monociliated to multiciliated cells / Nathalie Delgehyr, Alice Meunier, Marion Faucourt, Montserrat Bosch Grau, Laetitia Strehl, Carsten Janke, Nathalie Spassky -- Observing planar cell polarity in multiciliated mouse airway epithelial cells / Eszter K. Vladar, Yin Loon Lee, Tim Stearns, Jeffrey D. Axelrod -- Imaging and analyzing primary cilia in cardiac cells / Nicolas Diguet, Jean-Francois Le Garrec, Tommaso Lucchesi, Sigolene M. Meilhac -- Cilia in photoreceptors / Linjing Li, Manisha Anand, Kollu Nageswara Rao, Hemant Khanna -- Analysis of primary cilia in the developing mouse brain / Judith T.M.L. Paridaen, Wieland B. Huttner, Michaela Wilsch-Brauninger -- In vivo investigation of cilia structure and function using Xenopus / Eric R. Brooks, John B. Wallingford -- A quantitative approach to study endothelial cilia bending stiffness during blood flow mechanodetection in-vivo / Francesco Boselli, Jacky G. Goetz, Gilles Charvin, Julien Vermot -- Quantitative description of fluid flows produced by left-right cilia in zebrafish / Craig Fox, M. Lisa Manning, Jeffrey D. Amack -- Efficient live fluorescence imaging of intraflagellar transport in mammalian primary cilia / Hiroaki Ishikawa, Wallace F. Marshall -- Analysis of soluble protein entry into primary cilia using semipermeabilized cells / David K. Breslow, Maxence V. Nachury -- Methods for imaging individual cilia in living echinoid embryos / Robert L. Morris, Hans W. Pope, Adam N. Sholi, Leah M. Williams, Chelsea R. Ettinger, Gwendolyn M. Beacham, Tatsushi Shintaku, Zachary D. Abbott, Elyse M. Doherty -- The planarian Schmidtea mediterranea as a model for studying motile cilia and multiciliated cells / Cyril Basquin, Anne-Marie Orfila, Juliette Azimzadeh -- Quantitative analysis of flagellar proteins in Drosophila sperm tails / Teresa Mendes Maia, Perrine Paul-Gilloteaux, Renata Basto -- Imaging cilia in Drosophila melanogaster / Jennifer Vieillard, Jean-Luc Duteyrat, Elisabeth Cortier, Benedicte Durand -- Studying G protein-coupled receptors: Immunoblotting, immunoprecipitation, phosphorylation, surface labeling, and cross-linking protocols / Kasturi Pal, Hemant Badgandi, Saikat Mukhopadhyay -- Image analysis of Caenorhabditis elegans ciliary transition zone structure, ultrastructure, molecular composition and function / Anna A. W. M. Sanders, Julie Kennedy, Oliver E. Blacque -- Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii / Huawen Lin, Susan K. Dutcher -- Axonemal motility in Chlamydomonas / Ken-ichi Wakabayashi, Ritsu Kamiya -- Posttranscriptional control over rapid development and ciliogenesis in marsilea / Stephen M. Wolniak, Thomas C. Boothby, Corine M. van der Weele -- Total internal reflection fluorescence microscopy of intraflagellar transport in tetrahymena thermophila / Yu-Yang Jiang, Karl Lechtreck, Jacek Gaertig -- Ciliary heterogeneity within a single cell : the paramecium model / Anne Aubusson-Fleury, Jean Cohen, Michel Lemullois -- Imaging intraflagellar transport in trypanosomes / Julien Santi-Rocca, Nicolas Chenouard, Cécile Fort, Thibault Lagache, Jean-Christophe Olivo-Marin, Philippe Bastin -- Scanning and three-dimensional electron microscopy methods for the study of trypanosoma brucei and leishmania mexicana flagella / Eva Gluenz, Richard John Wheeler, Louise Hughes, Sue Vaughan.
  • 2008 ScienceDirect
    edited by Bhanu P. Jena.
    Also available: Print – 2008
  • 2009 Springer Protocols
    edited by David J. Carroll.
    Expression of exogenous mRNA in Xenopus laevis embryos for the study of cell cycle regulation / Jill C. Sible and Brian N. Wroble -- Use of luciferase chimaera to monitor PLC[zeta] expression in mouse eggs / Karl Swann ... [et al.] -- Analysis of 14-3-3 family member function in Xenopus embryos by microinjection of antisense morpholino oligos / Jeffrey M.S. Lau and Anthony J. Muslin -- A microinjectable biological system, the Xenopus oocyte, as an approach to understanding signal transduction protein function / Katia Cailliau and Edith Browaeys-Poly -- Combining microinjection and immunoblotting to analyse MAP kinase phosphorylation in single starfish oocytes and eggs / David J. Carroll and Wei Hua -- Analysis of signaling pathways in zebrafish development by microinjection / William H. Kinsey -- Protein inhibition by microinjection and RNA-mediated interference in tissue culture cells : complementary approaches to study protein function / Jane R. Stout, Rania S. Rizk, and Claire E. Walczak -- DNA delivery by microinjection for the generation of recombinant mammalian cell lines / Sebastien Chenuet ... [et al.] -- Bacteriophage [phi]C31 integrase mediated transgenesis in Xenopus laevis for protein expression at endogenous levels / Bryan G. Allen and Daniel L. Weeks -- Germline transformation of Caenorhabditis elegans by injection / Pavan Kadandale, Indrani Chatterjee, and Andrew Singson -- Quantitative microinjection of mouse oocytes and eggs / Douglas Kline -- Microinjection of follicle-enclosed mouse oocytes / Laurinda A. Jaffe ... [et al.] -- Functional studies of regulatory genes in the sea urchin embryo / Vincenzo Cavalieri, Maria Di Bernardo, and Giovanni Spinelli -- Exploring the cytoskeleton during intracytoplasmic sperm injection in humans / Vanesa Y. Rawe and Héctor Chemes -- Somatic cell nuclear transfer in the mouse / Satoshi Kishigami and Teruhiko Wakayama.
  • pt. A-C, 2014 ScienceDirect
    Pt. A ScienceDirect
    Pt. B ScienceDirect
    Pt. C ScienceDirect
    edited by Matthieu Piel and Manuel Théry.
    "Micropatterning refers generally to techniques which provide an experimental control over the chemical, physical, or geometrical properties of materials at the micron or submicron scale, and are thus used to produce spatial patterns of these properties. These techniques, which were often originally designed for application in microelectronics, have spread over most areas of science, including biology. They have proved particularly useful for cell biology, bridging the gap between the Petri-dish and complex 3D assays and tissues."--Preface.
  • 2010 Springer
    Zhiguo Wang, Baofeng Yang.
  • 2009. Springer
    Zhiguo Wang.
  • 2015 Springer
    Gaetano Santulli, editor.
    1. A fleeting glimpse inside microRNA, epigenetics and micropeptidomics -- 2. The microRNA machinery -- 3. microRNAs in mitochondria: an unexplored niche -- 4. microRNAs distinctively regulate vascular smooth muscle and endothelial cells: functional implications in angiogenesis, atherosclerosis, and in-stent restenosis -- 5. Mechanistic role of microRNAs in coupling lipid metabolism and atherosclerosis -- 6. microRNAs in pancreatic β-cell physiology -- 7. microRNA and cardiac regeneration -- 8. microRNAs and endometrial pathophysiology -- 9. microRNA and NF-kappa B -- 10. microRNAs: key players in hematopoiesis -- 11. Regulatory roles of miRNAs in aging -- 12. Computational prediction of microRNA targets
    Also available: Print – 2015
  • 2015 Springer
    Catherine M. Greene, editor.
    The book serves as a comprehensive resource for scientists and clinicians studying the role of non-coding RNAs in inflammation (viral infections, wound inflammation), human inflammatory diseases (i.e. rheumatoid arthritis, Crohnℓ́ℓs disease, diabetes), and innate immunity. It provides a universal reference work comprising both basic and specialized information. Given that ncRNAs represent new therapeutic targets, this volume will also be of interest to industrial biomedical researchers and those involved in drug development.
    Also available: Print – 2015
  • 2011 Springer Protocols
    edited by Tamas Dalmay.
    In situ detection of microRNAs in animals / Dylan Sweetman -- Detection of microRNAs in plants by in situ hybridisation / Eva Varallyay and Zoltan Havelda -- Detecting sRNAs by northern blotting / Sara Lopez-Gomollon -- Profiling microRNAs by real-time PCR / Nanna Jacobsen, Ditte Andreasen, and Peter Mouritzen -- Detection of small RNAs and microRNAs using deep sequencing technology / Ericka R. Havecker -- Detection of microRNAs in prostate cancer cells by mircoRNA array / Xiaoqing Tang [and others] -- MicroRNA knock down by cholesterol-conjugated antisence oligos in mouse organ culture / Sharon Kredo-Russo and Eran Hornstein -- Protocols for use of homologous recombination gene targeting to produce microRNA mutants in drosophila / Ya-Wen Chen, Ruifen Weng, and Stephen M. Cohen -- Engineering elements for gene silencing : the artificial microRNAs technology / Pablo Andres Manavella and Ignacio Rubio-Somoza -- Mimicry technology : suppressing small RNA activity in plants / Ignacio Rubio-Somoza and Pablo Andres Manavella -- Experimental validation of microRNA targets using a luciferase reporter system / Francisco E. Nicolas -- Experimental identification of microRNA targets by immunoprecipitation of argonaute protein complexes / Michaela Beitzinger and Gunter Meister -- Comprehensive identification of miRNA target sites in live animals / Dimitrios G. Zisoulis, Gene W. Yeo, and Amy E. Pasquinelli -- Target validation of plant microRNAs / Cesar Llave [and others] -- High-throughput sequencing-based methodology to identify all uncapped and cleaved RNA molecules in eukaryotic genomes / Matthew W. Endres, Rebecca T. Cook, and Brian D. Gregory.
  • 2011 Springer Protocols
    edited by Anne Straube.
    How to measure microtubule dynamics? / Anne Straube -- Purification of tubulin from porcine brain / Christopher Gell [and others] -- Purification of tubulin from the fission yeast Schizosaccharomyces pombe / Douglas R. Drummond [and others] -- Generation of differentially polyglutamylated microtubules / Benjamin Lacroix and Carsten Janke -- Preparation of pure tyrosinated or detyrosinated tubulin isoforms / Laurence Lafanechère and Didier Job -- [alpha][beta]-tubulin and microtubule-binding assays / Jaime N. Campbell and Kevin C. Slep -- Analysis of microtubule assembly kinetics using turbidimetry / Felicia Gaskin -- Isolation of centrosomes from cultured cells / Simone Reber -- Preparation of dual-color polarity-marked fluorescent microtubule seeds / Miho Katsuki, Etsuko Muto, and Robert A. Cross -- Reconstitution and quantification of dynamic microtubule end tracking in vitro using TIRF microscopy / Ivo A. Telley, Peter Bieling, and Thomas Surrey -- Force generation by dynamic microtubules in vitro / Svenja-Marei Kalisch, Liedewij Laan, and Marileen Dogterom -- Variations on the single-molecule assay for microtubule-associated proteins and kinesins / Susanne Bechstedt [and others] -- Analysing the ATP turnover cycle of microtubule motors / Claire T. Friel, Clive R. Bagshaw, and Jonathon Howard -- Cryo-electron tomography of microtubules assembled in vitro from purified components / Frédéric M. Coquelle [and others] -- Kinetochore microtubules, analyzed by correlated light and immunoelectron microscopy / Andreas Merdes and Jan De Mey -- Photoactivatable-GFP-[alpha]-tubulin as a tool to study microtubule plus-end turnover in living human cells / Catarina P. Samora and Andrew D. McAinsh -- Quantification of asymmetric microtubule nucleation at subcellular structures / Xiaodong Zhu and Irina Kaverina -- Investigating microtubule dynamic instability using microtubule-targeting agents / Stéphane Honore and Diane Braguer -- Laser ablation of the microtubule cytoskeleton : setting up and working with an ablation system / Nicola Maghelli and Iva M. Tolić-Nørrelykke -- Isolation, identification, and validation of microtubule-associated proteins from Drosophila embryos / Robin Antrobus and James G. Wakefield -- Isolation of novel +TIPs and their binding partners using affinity purification techniques / Ka Lou Yu [and others].
  • 2013 ScienceDirect
    edited by John J. Correia, Leslie Wilson.
    Site-specific fluorescent labeling of tubulin / Kamalika Muhkerjee, Susan L. Bane -- Measuring microtubule persistence length using a microtubule gliding assay / Douglas S. Martin -- Structural studies of the doublecortin family of MAPs / Frank Fourniol, Mylène Perderiset, Anne Houdusse, Carolyn Moores -- Detection and quantification of microtubule detachment from centrosomes and spindle poles / Anutosh Ganguly, Hailing Yang, Fernando Cabral -- Regulation of tubulin expression by micro-RNAs : implications for drug resistance / Sharon Lobert, Mary E. Graichen -- Determining the structure-mechanics relationships of dense microtubule networks with confocal microscopy and magnetic tweezers-based microrheology / Yali Yang, Megan T. Valentine -- Studying mitochondria and microtubule localization and dynamics in standardized cell shapes / Andrea Pelikan, James Sillibourne, Stephanie Miserey-Lenkei, Frederique Carlier-Grynkorn, Bruno Goud, Phong T. Tran -- Going solo : measuring the motions of microtubules with an in vitro assay for TIRF microscopy / Kris Leslie, Niels Galjart -- Analysis of microtubules in isolated axoplasm from the squid giant axon / Yuyu Song, Scott T. Brady -- Imaging GTP-bound tubulin : from cellular to in vitro assembled microtubules / Hélène de Forges, Antoine Pilon, Christian Poüs, Franck Perez -- Tubulin-specific chaperones : components of a molecular machine that assembles the ... heterodimer / Guoling Tian, Nicholas J. Cowan -- Heterotrimeric G proteins and microtubules / Witchuda Saengsawang, Mark M. Rasenick -- Purification and biophysical analysis of microtubule-severing enzymes in vitro / Juan Daniel Diaz-Valencia, Megan Bailey, Jennifer L. Ross -- Measurement of in vitro microtubule polymerization by turbidity and fluorescence / Matthew Mirigian, Kamalika Mukherjee, Susan L. Bane, Dan L. Sackett -- Live-cell imaging of microtubules and microtubule-associated proteins in arabidopsis thaliana / Jessica Lucas -- Investigating tubulin posttranslational modifications with specific antibodies / Maria M. Magiera, Carsten Janke -- Purification and assembly of bacterial tubulin BtubA/B and constructs bearing eukaryotic tubulin sequences / José M. Andreu, María A. Oliva -- Microtubule-associated proteins and tubulin interaction by isothermal titration calorimetry / P.O. Tsvetkov, P. Barbier, G. Breuzard, V. Peyrot, F. Devred -- Methods for studying microtubule binding site interactions : zampanolide as a covalent binding agent / Jessica J. Field, Enrique Calvo, Peter T. Northcote, John H. Miller, Karl-Heinz Altmann, José Fernando Díaz -- Studying kinetochore-fiber ultrastructure using correlative light-electron microscopy / Daniel G. Booth, Liam P. Cheeseman, Ian A. Prior, Stephen J. Royle -- Fluorescence-based assays for microtubule architecture / Susanne Bechstedt, Gary L. Brouhard -- Structure-function analysis of yeast tubulin / Anna Luchniak, Yusuke Fukuda, Mohan L. Gupta -- Using MTBindingSim as a tool for experimental planning and interpretation / Julia T. Philip, Aranda R. Duan, Emily O. Alberico, Holly V. Goodson -- Imaging individual spindle microtubule dynamics in fission yeast / Judite Costa, Chuanhai Fu, Viktoriya Syrovatkina, Phong T. Tran.
  • 2010 ScienceDirect
    edited by Leslie Wilson, John J. Correia.
    sect. I. Isolation and biochemistry of tubulin and characterization of antibodies and isotypes -- Preparation of microtubule protein and purified tupbulin from bovine brain by cycles of assembly and disassembly and phosphocellulose chromatography / Herbert P. Miller, Leslie Wilson -- Isolating tubulin from nonneural sources / Dan L. Sackett, Karl A. Werbovetz, Naomi S. Morrissette -- Characterization of anti-B-tubulin antibodies / Anthony J. Spano, Anthony Frankfurter -- Expression profliing of tubulin isotypes and microtubule-interacting proteins using real-time polymerase chain reaction / Sharon Lobert, Laree Hiser, John J. Correia -- Nondenaturing electrophoresis as a tool to investigate tabulin complexes / Monica Lopez Fanarago... [et al.] -- Mass spectrometry analysis of c-terminal postranslational modifications of tubulins / Virginie Redeker -- Methods in tubulin proteomics / Leah M. Miller... [et al.] -- sect. II. Microtubule structure and dynamics -- Cryo-em studies of microtubule structural intermediates and kinetochore-microtubule interactions / Eva Nogales, Vincent H. Rame, Hong-Wei Wang -- High-resolution imaging of microtubules and cytoskeleton structures by atomic force microscopy / Loic Hamon, Patrick A. Curmi, David Patre -- Using computational modeling to understand microtubule dynamics : a primer for cell biologists / Holly V. Goodson, Ivan V. Gregoretti -- Analysis of dynamic instability of steady-state microtubules in vitro by video-enchanced differential interference contrast microscopy with an appendix by Emin Oroudjev / Mythili Yenjerla, Manu Lopus, Leslie Wilson -- Nanometer-resolution microtubule polymerization assays using optical tweezers and microfabricated barriers/ Blake d. Charlebois, Henry T. Schek III, Alan J. Hunt -- Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy / Christopher Gell... [et al.] -- Studying kinesin motors by optical 3D-nanometry in gliding motility assays / Bert Nitzsche... [et al.] -- sect. III. Drugs -- Analysis of tubulin oligomers by analytical ultricentrifugation / John J. Correia -- Determination of drug binding to mactrotubules in vitro / Jennifer A. Smith, Mary Ann Jordan -- Fluorescence spectroscopic methods to analyze drug-tubulin interactoins / Bhabatarak Bhattacharyya, Sonia Kapoor, Dulal Panda -- Tubulin polymerizatin microassay used to compare ligand eficacy / Ashley Davis... [et al.] -- Fluorescent taxoid probes for microtubule research / Isabel Barasoain, J. Fernando Diaz, Jose M. Andrea -- Binding of vinca domain agents to tubulin : structural and biomedical studies / Anthony Cormier... [et al.] -- Measurement of ligand binding to tubulin by sulfhydryl reactivity / Adrian Begaye, Dan L. Sackett -- sect. IV. Interactions with motors and MAPs -- Probing interactions of tubulin with small molecules, peptides, and protein fragments by solution nuclear magnetic resonance / Marie-Jeanne Clement... [et al.] -- Microtubule and MAPs : thermodynamics of complex formation by AUC, ITC, fluorescence, and NMR / Francois Devred... [et al.] -- Quantitative analysis of MAP-mediated regulation of microtubule dynamic instability in vitro--focus on tau / Erkan Kiris, Donovan Ventimiglia, Stuart C. Feinstein -- Structure and dynamics of the kinesin-Microtubule interaction revealed by fluorescence polarization microscopy / Hernando Sosa, Ana B. Asenjo, Erwin J.G. Peterman -- Multiple collor single molecule TIRF imaging and tracking of MAPs and motors / Jennifer L. Ross, Ram Dixit -- Studying plus-end tracking at single molecule resolution using TIRF microscopy / Ram Dixit, Jennifer L. Ross -- Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor, and +TIP dynamics / Peter Bieling... [et al.] --
  • 2010 ScienceDirect
    edited by Lynne Cassimeris and Phong Tran.
  • 2012 Springer
    Mine Ergüven, Takashi Muramatsu, Ayhan Bilir, editors.
    pt. 1. GENERAL ASPECTS OF MIDKINE -- General Information on Midkine / Takashi Muramatsu -- The Role of Midkine in Neural Development and Patterning / Sheng Yao and Christoph Winkler -- Midkine: A Player in Innate Immunity / Sara L. Nordin and Arne Egesten -- Midkine/P63 Axis in Regulation of Epithelial-Mesenchymal Transition / Yiping Huang and Edward A. Ratovitski -- Immunochemical Methods for the Detection of Midkine in Samples of Human Origin / Stefan Sommerschuh, Michael Kirchner and Nicole Gliese -- Circulating Midkine in Malignant, Inflammatory, and Infectious Diseases: A Systematic Review / Malgorzata Krzystek-Korpacka and Malgorzata Matusiewicz -- pt. 2. MIDKINE AND INFLAMMATION -- Midkine in Inflammation and Inflammatory Diseases / Nuray Yazıhan, Mehtap Kacar Kocak and Ethem Akcıl -- Rheumatoid Arthritis and Midkine / Hisako Muramatsu, Kiyoko Maruyama and Naoki Ishiguro -- pt. 3. MIDKINE AND THE CARDIOVASCULAR SYSTEM -- Midkine and Heart Failure / Mitsuru Horiba and Kenji Kadomatsu -- Vascular Restenosis and Midkine / Kenji Kadomatsu -- The Potential of MK Through the Renin-Angiotensin System / Tomoki Kosugi and Kenji Kadomatsu -- pt. 4. MIDKINE AND THE NERVOUS SYSTEM -- Midkine and Multiple Sclerosis / Hideyuki Takeuchi -- Midkine Gene Transfer in Brain Infarction / Hiroaki Ooboshi, Eiichi Ishikawa, Junichi Takada, Takashi Shichita and Yasuhiro Kumai -- Midkine in Psychiatric and Neurodegenerative Diseases / Eiji Shimizu and Daisuke Matsuzawa -- pt. 5. MIDKINE AND THE UROGENITAL SYSTEM, REPRODUCTION AND AGING -- Role of Midkine in Nephrogenesis and Kidney Disease / Waichi Sato and Kenji Kadomatsu -- Midkine, a Factor Promoting Cytoplasmic Maturation of Oocytes / Masayasu Yamada, Yuuki Isaji and Shuntaro Ikeda -- Endometriosis May Be Promoted by Midkine: Possible Involvement of Ovarian Follicles / Yutaka Osuga, Yasushi Hirota, Kaori Koga, Osamu Yoshino and Tetsuya Hirata -- Stem Cells and Midkine / Hisako Muramatsu, Peng Zou and Takashi Muramatsu -- Midkine in Skeletal Physiology / Astrid Liedert, Thorsten Schinke, Anita Ignatius and Michael Amling -- pt. 6. MIDKINE AND CANCER -- Midkine and Chemoresistance in Cancers / Yoshifumi Takei and Kenji Kadomatsu -- A Gene Medicine with the Midkine-mediated Transcriptional Regulation as New Cancer Therapeutics / Masatoshi Tagawa, Kiyoko Kawamura, Ling Yu, Yuji Tada and Kenzo Hiroshima, et al. -- Midkine as a Tumor-Shared Antigen / Jerome Kerzerho, Florence Anne Castelli and Bernard Maillère -- Midkine in Prostate Cancer / Zongbing You -- Midkine Is a Potential Therapeutic Target of Hepatocellular Carcinoma / Li Cheng Dai, Xing Yao and Jing Zhong -- Midkine in Glioblastoma with the Modulator Role Switching Autophagy to Cell Death or Cell Survival / Mine Ergüven and Ayhan Bilir -- Colon Cancer and Midkine / Özgür Kemik, Ahu Sarbay Kemik and A. Cumhur Dülger -- Osteosarcoma and Midkine / Tadashi Kaname, Kumiko Yanagi and Hiroki Maehara.
  • 2007 Springer
    edited by Stephen W. Schaffer and M.-Saadeh Suleiman.
    Regulation of mitochondrial respiration in heart muscle / Ilmo Hassinen -- Regulation of fatty acid oxidation of the heart / Clifford D.L. Folmes and Gary D. Lopaschuk -- Regulation of mitochondrial fuel handling by the peroxisome proliferator-activated receptors / Mary C. Sugden and Mark J. Holness -- Molecular structure of the mitochondrial citrate transport protein / Ronald S. Kaplan and June A. Mayor -- Regulation of pyruvate and amino acid metabolism / Thomas C. Vary, Wiley W. Souba, and Cristopher J. Lynch -- Amino acids and the mitochondria / Nicola King -- Mechanotransduction of shear-stress at the mitochondria / Abu-Bakr Al-Mehdi -- Formation of reactive oxygen species in mitochondria / Julio F. Turrens -- Mitochondrial calcium : role in the normal and ischaemic/reperfused myocardium / Elinor J. Griffiths ... [et al.] -- Mitochondrial ion channels / Brian O'Rourke -- The mitochondrial permeability transition pore : from molecular mechanism to reperfusion injury and cadioprotection / Andrew P. Halestrap, Samantha J. Clarke, and Igor Khalilin -- The apoptotic mitochondrial pathway : modulators, interventions, and clinical implications / M.-Saadeh Suleiman and Stephen W. Schaffer -- The role of mitochondria in necrosis following myocardial ischemia-reperfusion / Elizabeth Murphy and Charles Steenbergen -- Mitochondria and their role in ischemia/reperfusion injury / Sebastian Phillip, James M. Downey, and Michael V. Cohen -- Mitochondrial DNA damage and repair / Inna N. Shokolenko, Susan P. Ledoux, and Glenn L. Wilson.
  • 2009 Springer
    Keshav K. Singh, Leslie C. Costello, editors.
  • 2007 ScienceDirect
    edited by Liza A. Pon, Eric A. Schon.
    Also available: Print – 2007
  • 2007. Springer Protocols
    edited by Dario Leister, Johannes Herrmann.
    Offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike.
  • 2012 Springer Protocols
    edited by Carlos M. Palmeira, António J. Moreno.
    Overview of mitochondrial bioenergetics / Vitor M.C. Madeira -- Evaluation of respiration with Clark type electrode in isolated mitochondria and permeabilized animal cells / Ana M. Silva and Paulo J. Oliveira -- High-resolution respirometry : OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle / Dominik Pesta and Erich Gnaiger -- High-throughput analysis of mitochondrial oxygen consumption / James Hynes, Rachel L. Swiss, and Yvonne Will -- Modulation of cellular respiration by endogenously produced nitric oxide in rat hippocampal slices / Ana Ledo, R.M. Barbosa, and J. Laranjinha -- Mitochondrial membrane potential ([delta][psi]) fluctuations associated with the metabolic states of mitochondria / Carlos M. Palmeira and Anabela P. Rolo -- Safranine as a fluorescent probe for the evaluation of mitochondrial membrane potential in isolated organelles and permeabilized cells / Tiago R. Figueira ... [et al.] -- Fluorescence measurement of mitochondrial membrane potential changes in cultured cells / David G. Nicholls -- Phenomenological kinetic and control analysis of oxidative phosphorylation in isolated mitochondria / Vilmante Borutaite and Rasa Baniene -- Expression of uncoupling proteins in a mammalian cell culture system (HEK293) and assessment of their protein function / Martin Jastroch -- Measurement of proton leak and electron leak in isolated mitochondria / Charles Affourtit, Casey L. Quinlan, and Martin D. Brand -- Relation between mitochondrial membrane potential and ROS formation / Jan M. Suski ... [et al.] -- Use of a calcium-sensitive electrode for studies on mitochondrial calcium transport / António J.M. Moreno and Joaquim A. Vicente -- Imaging mitochondrial calcium signalling with fluorescent probes and single or two photon confocal microscopy / Sean M. Davidson and Michael R. Duchen -- Mitochondrial permeability transition pore and calcium handling / Renee Wong, Charles Steenbergen, and Elizabeth Murphy -- Imaging of mitochondrial pH using SNARF-1 / Venkat K. Ramshesh and John J. Lemasters -- Redox equivalents and mitochondrial bioenergetics / James R. Roede, Young-Mi Go, and Dean P. Jones -- NMR methodologies for studying mitochondrial bioenergetics / Tiago C. Alves, Ivana Jarak, and Rui A. Carvalho -- Computational modeling of mitochondrial function / Sonia Cortassa and Miguel A. Aon.
  • 2012 Springer Protocols
    edited by Lee-Jun C. Wong.
    Mitochondrial DNA mutations : an overview of clinical and molecular aspects / William J. Craigen -- Nuclear gene defects in mitochondrial disorders / Fernando Scaglia -- Diagnostic challenges of mitochondrial disorders : complexities of two genomes / Brett H. Graham -- Biochemical analyses of the electron transport chain complexes by spectrophotometry / Ann E. Frazier and David R. Thorburn -- Measurement of mitochondrial oxygen consumption using a Clark electrode / Zhihong Li and Brett H. Graham -- Mitochondrial respiratory chain : biochemical analysis and criterion for deficiency in diagnosis / Manuela M. Grazina -- Assays of pyruvate dehydrogenase complex and pyruvate carboxylase activity / Douglas Kerr, George Grahame, and Ghunwa Nakouzi -- Assessment of thymidine phosphorylase function : measurement of plasma thymidine (and deoxyuridine) and thymidine phosphorylase activity / Ramon Martí, Luis C. López, and Michio Hirano -- Measurement of mitochondrial dNTP pools / Ramon Martí, Beatriz Dorado, and Michio Hirano -- Measurement of oxidized and reduced coenzyme Q in biological fluids, cells, and tissues : an HPLC-EC method / Peter H. Tang and Michael V. Miles -- Assay to measure oxidized and reduced forms of CoQ by LC-MS/MS / Si Houn Hahn, Sandra Kerfoot, and Valeria Vasta -- Morphological assessment of mitochondrial respiratory chain function on tissue sections / Kurenai Tanji -- Blue native polyacrylamide gel electrophoresis : a powerful diagnostic tool for the detection of assembly defects in the enzyme complexes of oxidative phosphorylation / Scot C. Leary -- Radioactive labeling of mitochondrial translation products in cultured cells / Florin Sasarman and Eric A. Shoubridge -- Transmitochondrial cybrids : tools for functional studies of mutant mitochondria / Sajna Antony Vithayathil, Yewei Ma, and Benny Abraham Kaipparettu -- Fluorescence-activated cell sorting analysis of mitochondrial content, membrane potential, and matrix oxidant burden in human lymphoblastoid cell lines / Stephen Dingley, Kimberly A. Chapman, and Marni J. Falk -- Molecular profiling of mitochondrial dysfunction in Caenorhabditis elegans / Erzsebet Polyak, Zhe Zhang, and Marni J. Falk -- Analysis of common mitochondrial DNA mutations by allele-specific oligonucleotide and southern blot hybridization / Sha Tang [and others] -- Sequence analysis of the whole mitochondrial genome and nuclear genes causing mitochondrial disorders / Megan L. Landsverk, Megan E. Cornwell, and Meagan E. Palculict -- Utility of array CGH in molecular diagnosis of mitochondrial disorders / Jing Wang and Mrudula Rakhade -- Quantification of mtDNA mutation heteroplasmy (ARMS qPCR) / Victor Venegas and Michelle C. Halberg -- Measurement of mitochondrial DNA copy number / Victor Venegas and Michelle C. Halberg -- Determination of the clinical significance of an unclassified variant / Victor Wei Zhang and Jing Wang.
  • 2013 Springer
    Lee-Jun C. Wong, editor.
    Mitochondrial cytopathies are mutations in the inherited maternal mitochondrial genome, or the nuclear DNA-mutation. Mitochondrial respiratory chain disorders (RCD) are a group of genetically and clinically heterogeneous diseases, due to the fact that protein components of the respiratory chain are encoded by both mitochondrial and nuclear genomes and are essential in all cells. In addition, the biogenesis, structure and function of mitochondria, including DNA replication, transcription, and translation, all require nuclear encoded genes. Since mitochondria are present in every cell, every tissue, mitochondrial disorders usually affects multiple organs. Mitochondrial Disorders Caused by Nuclear Genes discusses the biochemical, molecular, clinical, and genetic aspects of complex dual genome mitochondrial disorders. Chapters include genes involved in mitochondrial DNA biogenesis and maintenance of mitochondrial DNA integrity, complex subunits and assembly genes, and mitochondrial protein translation related diseases.
  • 2013 Springer
    Justin C. St. John, editor.
    1. Clinical Approach to the Diagnosis of Mitochondrial Disease / Dominic Thyagarajan, p. 1-23 -- 2. Mitochondrial DNA Mutations and Their Effects on Complex I Biogenesis: Implications for Metabolic Disease / Matthew McKenzie, p. 25-47 -- 3. Embryonic Stem Cells: A Signalling Perspective / Marios P. Stavridis, p. 49-68 -- 4. From Oocytes and Pluripotent Stem Cells to Fully Differentiated Fates: (Also) a Mitochondrial Odyssey / João Ramalho-Santos, Ana Sofia Rodrigues, p. 69-86 -- 5. From Pluripotency to Differentiation: The Role of mtDNA in Stem Cell Models of Mitochondrial Diseases / João Facucho-Oliveira, Tejal Kulkarni, p. 87-118 -- 6. The Role of Mitochondrial DNA in Tumorigenesis / Ka Yu Yeung, Adam Dickinson, p. 119-155 -- 7. Assisted Reproductive Technologies: The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next / Richard D. W. Kelly, Arsalan Mahmud, p. 157-183.
  • 2014 Springer
    Viswanathan Natarajan, Narasimham L. Parinandi, editors.
    Mitochondria, often referred to as the 'powerhouses' of the cell, generate adenosine triphosphate (ATP) by oxidative phosphorylation or OXPHOS, and maintain cellular homeostasis. In addition to generating ATP, mitochondria are involved in regulation of cell cycle, proliferation, free radical production, innate immune responses and apoptosis. Mitochondrial Function in Lung Health and Disease fills the current gap in the literature and outlines the growing clinical relevance of mitochondrial dysfunction. Currently, there is no overview on the role of mitochondria in pulmonary diseases and this volume focuses on the mitochondrial metabolism, redox signaling, and mechanisms of mitochondrial pathways in lung injury, inflammation, repair and remodeling. Furthermore, in addition to their well-recognized role in cellular energy production and apoptosis, mitochondria appear to play a role in many respiratory diseases and lung cancer. Chapters are written by top notch researchers and clinicians and outline the evidence for mitochondrial biogenesis in inhalational lung injury, COPD and asthma.
  • 2008 Springer
    Anna Gvozdjáková, editor.
  • 2015 Springer Protocols
    edited by Volkmar Weissig, Marvin Edeas.
    This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. "Mitochondrial Medicine: Volume I, Probing Mitochondrial Function" focuses on methods being used for the assessment of mitochondrial function under physiological conditions as well as in healthy isolated mitochondria. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, "Mitochondrial Medicine" provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.
  • 2015 Springer Protocols
    edited by Volkmar Weissig, Marvin Edeas.
    This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. "Mitochondrial Medicine: Volume II, Manipulating Mitochondrial Function" describes techniques developed for manipulating and assessing mitochondrial function under general pathological conditions and specific disease states. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, "Mitochondrial Medicine" provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.
  • 2012 Springer
    Bernhard Kadenbach, editor.
    Introduction to Mitochondrial Oxidative Phosphorylation / Bernhard Kadenbach -- Mitochondrial Dynamics: The Intersection of Form and Function / Andrew Ferree and Orian Shirihai -- Biogenesis of Mitochondrial Proteins / Johannes M. Herrmann, Sebastian Longen, Daniel Weckbecker and Matthieu Depuydt -- Assembly Factors of Human Mitochondrial Respiratory Chain Complexes: Physiology and Pathophysiology / Daniele Ghezzi and Massimo Zeviani -- Supramolecular Organisation of the Mitochondrial Respiratory Chain: A New Challenge for the Mechanism and Control of Oxidative Phosphorylation / Giorgio Lenaz and Maria Luisa Genova -- Molecular Mechanisms of Superoxide Production by the Mitochondrial Respiratory Chain / Stefan Dröse and Ulrich Brandt -- Studies on the Function and Regulation of Mitochondrial Uncoupling Proteins / Richard K. Porter -- Evolution of the Couple Cytochrome c and Cytochrome c Oxidase in Primates / Denis Pierron, Derek E. Wildman, Maik Hüttemann, Thierry Letellier and Lawrence I. Grossman -- Reaction Mechanism of Mammalian Mitochondrial Cytochrome c Oxidase / Shinya Yoshikawa, Kazumasa Muramoto and Kyoko Shinzawa-Itoh -- Phosphorylation of Mammalian Cytochrome c and Cytochrome c Oxidase in the Regulation of Cell Destiny: Respiration, Apoptosis, and Human Disease / Maik Hüttemann, Icksoo Lee, Lawrence I. Grossman, Jeffrey W. Doan and Thomas H. Sanderson -- Individual Biochemical Behaviour Versus Biological Robustness: Spotlight on the Regulation of Cytochrome c Oxidase / Rabia Ramzan, Petra Weber, Bernhard Kadenbach and Sebastian Vogt -- Bigenomic Regulation of Cytochrome c Oxidase in Neurons and the Tight Coupling Between Neuronal Activity and Energy Metabolism / Margaret T. T. Wong-Riley -- Cytochrome c Oxidase and Its Role in Neurodegeneration and Neuroprotection / Susanne Arnold -- The Many Clinical Faces of Cytochrome c Oxidase Deficiency / Salvatore DiMauro, Kurenai Tanji and Eric A. Schon.
  • 2015 Springer Protocols
    edited by Carlos M. Palmeira, Anabela P. Rolo.
    Isolation of crude mitochondrial fraction from cells / Mariusz R. Wieckowski and Lech Wojtczak -- Isolation of mitochondria from liver and extraction of total RNA and protein : analyses of MicroRNA and protein expressions / Pedro M. Borralho, Clifford J. Steer, and Cecília M.P. Rodrigues -- PCR based determination of mitochondrial DNA copy number in multiple species / John P. Rooney [and eight others] -- NAD⁺ content and its role in mitochondria / Wei Li and Anthony A. Sauve -- Measuring PGC-1[alpha] and its acetylation status in mouse primary myotubes / Ana P. Gomes and David A. Sinclair -- Measurement of mitochondrial oxygen consumption rates in mouse primary neurons and astrocytes / Sofia M. Ribeiro, Alfredo Giménez-Cassina, and Nika N. Danial -- Determination of oxidative phosphorylation complexes activities / João S. Teodoro, Carlos M. Palmeira, and Anabela P. Rolo -- Histoenzymatic methods for visualization of the activity of individual mitochondrial respiratory chain complexes in the muscle biopsies from patients with mitochondrial defects / Agnieszka Karkucinska-Wieckowska, Maciej Pronicki, and Mariusz R. Wieckowski -- A method aimed at assessing the functional consequences of the supramolecular organization of the respiratory electron transfer chain by time- resolved studies / Fabrice Rappaport -- BN-PAGE-based approach to study thyroid hormones and mitochondrial function / Elena Silvestri [and three others] -- Detection of UCP1 protein and measurements of dependent GDP-sensitive proton leak in non- phosphorylating thymus mitochondria / Kieran J. Clarke [and three others] -- Exploring liver mitochondrial function by ¹³C-stable isotope breath tests : implications in clinical biochemistry / Ignazio Grattagliano [and five others] -- Following mitochondria dynamism : confocal analysis of the organelle morphology / Francesca R. Mariotti, Mauro Corrado, and Silvia Campello -- Analysis of pro-apoptotic protein trafficking to and from mitochondria / Ignacio Vega-Naredo [and four others] -- Mitophagy and mitochondrial balance / Simone Patergnani and Paolo Pinton.
  • 2012 CRCnetBASE
    edited by Sten Orrenius, Lester Packer, Enrique Cadenas.
    "This book examines a unique assembly of coordinated mitochondrial functions that are important in regulating cell function and are of relevance in a myriad of pathophysiological situations, such as age-related neurodegenerative disorders, cancer, metabolic syndrome, and cardiovascular disease. The text covers themes essential for the maintenance of mitochondrial activity, including electron transport and energy production, mitochondrial biogenesis and dynamics, mitochondrial signaling, and apoptosis and autophagy. The book features chapters that are reviews of the important topics covered and are written by an impressive cadre of internationally recognized scientist"--Provided by publisher.
  • 2014 Springer Protocols
    edited by David Sharp.
    Analysis of Mitotic Protein Dynamics and Function in Drosophila Embryos by Live Cell Imaging and Quantitative Modeling -- Rapid Measurement of Mitotic Spindle Orientation in Cultured Mammalian Cells -- Automated Segmentation of the First Mitotic Spindle in Differential Interference Contrast Micro copy Images of C. elegans Embryos -- Imaging the Mitotic Spindle by Spinning Disk Microscopy in Tobacco Suspension Cultured Cells -- Image-Based Computational Tracking and Analysis of Spindle Protein Dynamics -- The Use of Cultured Drosophila Cells for Studying the Microtubule Cytoskeleton -- Measuring Microtubule Growth and Gliding in C. elegans Embryos -- XenopusEgg Extracts as a Simplified Model System for Structure Function Studies of Dynein Regulators -- Covalent Immobilization of Microtubules on Glass Surfaces for Molecular Motor Force Measurements and Other Single-Molecule Assays -- An Improved Optical Tweezers Assay for Measuring the Force Generation of Single Kinesin Molecules -- Seeded Microtubule Growth for Cryo-electron Microscopy of End Binding Proteins -- The Segmentation of Microtubules in Electron Tomograms using Amira -- Manipulating Cell Shape by Placing Cells into Micro-fabricated Chambers -- Four color FISH for the Detection of Low Levels Aneuploidy in Interphase Cells.
  • 2003 CRCnetBASE
    edited by C. Pozrikidis.
  • 2012 Springer
    Robert B. Denman, editor.
    Introduction: Reminiscing on Models and Modeling / Robert B. Denman -- Probing Astrocyte Function in Fragile X Syndrome / Shelley Jacobs, Connie Cheng and Laurie C. Doering -- Neural Stem Cells / Maija Castrén -- Fragile X Mental Retardation Protein (FMRP) and the Spinal Sensory System / Theodore J. Price and Ohannes K. Melemedjian -- The Role of the Postsynaptic Density in the Pathology of the Fragile X Syndrome / Stefan Kindler and Hans-Jürgen Kreienkamp -- Behavior in a Drosophila Model of Fragile X / Sean M. McBride, Aaron J. Bell and Thomas A. Jongens -- Molecular and Genetic Analysis of the Drosophila Model of Fragile X Syndrome / Charles R. Tessier and Kendal Broadie -- Fragile X Mental Retardation Protein and Stem Cells / Abrar Qurashi, Xuekun Li and Peng Jin -- Manipulating the Fragile X Mental Retardation Proteins in the Frog / Marc-Etienne Huot, Nicolas Bisson, Thomas Moss and Edouard W. Khandjian -- Exploring the Zebra Finch Taeniopygia guttata as a Novel Animal Model for the Speech-Language Deficit of Fragile X Syndrome / Claudia Winograd and Stephanie Ceman -- Neuroendocrine Alterations in the Fragile X Mouse / Abdeslem El Idrissi, Xin Yan, William L'Amoreaux, W. Ted Brown and Carl Dobkin -- Taking STEPs Forward to Understand Fragile X Syndrome / Susan M. Goebel-Goody and Paul J. Lombroso -- Fmr-1 as an Offspring Genetic and a Maternal Environmental Factor in Neurodevelopmental Disease / Bojana Zupan and Miklos Toth -- Mouse Models of the Fragile X Premutation and the Fragile X Associated Tremor/Ataxia Syndrome / Michael R. Hunsaker, Gloria Arque, Robert F. Berman, Rob Willemsen and Renate K. Hukema -- Clinical Aspects of the Fragile X Syndrome / W. Ted Brown -- Fragile X Syndrome: A Psychiatric Perspective / Michael R. Tranfaglia -- Fragile X Syndrome and Targeted Treatment Trials / Randi Hagerman, Julie Lauterborn, Jacky Au and Elizabeth Berry-Kravis -- The Fragile X-Associated Tremor Ataxia Syndrome / Flora Tassone and Randi Hagerman -- Vignettes: Models in Absentia / Robert B. Denman.
    Also available: Print – 2012
  • 2010
    Mark Ping Chao.
    Growing evidence indicates that cancers are composed of functionally heterogeneous cells that are organized by a cellular hierarchy and are maintained by a stem cell-like population. Given their unique ability to initiate tumor growth, eradication of these cancer stem cells (CSC) is necessary for cure. Thus, the development of CSC targeted therapies is necessary to improve current clinical outcomes. This body of work identifies CD47 as a therapeutic target on CSC and bulk cells in several human malignancies. CD47 is a cell surface protein that functions to inhibit phagocytosis through binding its ligand SIRP[Alpha], expressed on phagocytes. Compared to normal cell counterparts, CD47 was highly expressed on several human tumors including acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), and non-Hodgkin lymphoma (NHL), suggesting that higher CD47 levels on tumors allow evasion of immune phagocytosis. Higher CD47 expression in these tumors correlated with a worse clinical prognosis and was an independent prognostic factor. Furthermore, a monoclonal blocking antibody against CD47 enabled phagocytosis of human AML, ALL, and NHL in vitro and eliminated disease in vivo. When combined with the Fc receptor-activating antibody, rituximab, anti-CD47 antibody therapy achieved high cure rates in pre-clinical models of NHL in contrast to partial responses with either agent alone. Lastly, anti-CD47 antibody treatment specifically targeted tumor but not normal cells, resulting in minimal toxicity in vitro and in vivo. The selective targeting of tumor cells by anti-CD47 antibody was due to the selective expression of the pro-phagocytic signal, calreticulin on tumor but not normal cells. In summary, this body of work identifies CD47 as a therapeutic target on several human cancers and that a monoclonal blocking antibody against CD47 has pre-clinical efficacy in eliminating these tumors through the promotion of phagocytosis.
  • 2007 CRCnetBASE
    edited by Michel M.J. Modo, Jeff W.M. Bulte.
    Methods for labeling non-phagocytic cells with MR contrast agents / Joseph A. Frank, Stasia A. Anderson, & Ali S. Arbab -- Cellular imaging of cell transplants / Michel Modo & Jeff W.M. Bulte -- Cellular and molecular imaging of the diabetic pancreas / Zdravka Medarova & Anna Moore -- Functional cellular imaging with manganese / Vincent van Meir & Annemie van der Linden -- Translating promising experimental approaches to clinical trials / Adrian D. Nunn -- An outlook on cellular and molecular MR imaging / Michel Modo & Jeff W.M. Bulte. What is molecular and cellular imaging? / Michel Modo & Jeff W.M. Bulte -- The physicochemical principles of MR contrast agents / Peter Caravan -- Paramagnetic contract agents / Silvio Aime ... [et al.] -- Superparamagnetic contrast agents / Claire Corot ... [et al.] -- Physical mechanism and applications of cest contrast agents / Mike McMahon ... [et al.] -- Paracest contrast agents / A. Dean Sherry & Mark Woods -- Genetic approaches for modulating MRI contrast / Eric Ahrens, Williams F. Goins, Clinton S. Robinson -- Molecular and functional mr imaging of cancer / Michael A. Jacobs ... [et al.] -- Molecular imaging of atherosclerosis with magnetic resonance / Karen C. Briley-Saebo ... [et al.] -- Molecular imaging of apoptosis / Mikko Kettunen & Kevin M. Brindle -- Molecular imaging of reporter genes / Keren Ziv ... [et al.] -- Pharmacological MRI as a molecular imaging technique / Y. Iris Chen & Bruce Jenkins -- Cellular imaging of the liver using contrast agents / N. Cem Balci & Sukru Mehmet Erturk -- Cellular imaging of macrophage activity in infection and inflammation / Martin Rausch ... [et al.] -- Cellular imaging of macrophage activity in stroke / Ralph Weber & Mathias Hoehn -- Magnetic resonance imaging in developmental biology / Cyrus Papan, J. Michael Tyszka, Russell E. Jacobs --
  • 2009 Springer
    Amittha Wickrema, Barbara Kee, editors.
  • 2010 Springer
    Roland Martin, Andreas Lutterotti (eds.).
    Also available: Print – 2010
  • 2008
    Bruce Alberts ... [et al.] ; with problems by John Wilson ... [et al.].
    Status: Not Checked OutLane Catalog Record
  • 2015
    Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter ; with problems by John Wilson, Tim Hunt.
    Status: Not Checked OutLane Catalog Record
    "As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts. As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced. Protein structures better illustrate structure-function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing open-ended questions highlighting "What We Don't Know," introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text. Thought-provoking end-of-chapter questions have been expanded to all chapters, including questions on developmental biology, tissues and stem cells, the immune system, and pathogens."-- Provided by publisher.
  • 2002 NCBI Bookshelf, Fulltext accessible by its search box
    Bruce Alberts ... [et al.].
    Also available: Print – 2002
    Also available: Print – 2002
  • 2008
    Bruce Alberts ... [et al.] ; with problems by John Wilson, Tim Hunt.
    Status: Not Checked OutLane Catalog Record
    Cells and genomes -- Cell chemistry and biosynthesis -- Proteins -- DNA, chromosomes, and genomes -- DNA replication, repair, and recombination -- How cells read the genome: from DNA to protein -- Control of gene expression -- Manipulating proteins, DNA, and RNA -- Visualizing cells -- Membrane structure -- Membrane transport of small molecules and the electrical properties of membranes -- Intracellular compartments and protein sorting -- Intracellular vesicular traffic -- Energy conversion: mitochondria and chloroplasts -- Mechanisms of cell communication -- The cytoskeleton -- The cell cycle -- Apoptosis -- Cell junctions, cell adhesion, and the extracellular matrix -- Cancer -- Sexual reproduction: meiosis, germ cells, and fertilization -- Development of multicellular organisms -- Specialized tissues, stem cells, and tissue renewal -- Pathogens, infection, and innate immunity -- The adaptive immune system. Tables: The genetic code, amino acids.
  • 2013
    Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott.
    Status: Not Checked OutLane Catalog Record
    Overview: Molecular Cell Biology presents the key concepts in cell biology and their experimental underpinnings. The authors, all world-class researchers and teachers, incorporate medically relevant examples where appropriate to help illustrate the connections between cell biology and health and human disease. As always, a hallmark of MCB is the use of experiments to engage students in the history of cell biology and the research that has contributed to the field. The book contains predominantly color illustrations, with some black-and-white illustrations.
  • 2000 NCBI Bookshelf
    Harvey Lodish ... [et al.].
    Also available: Print – 2000
  • 1987 ScienceDirect
    edited by Michael M. Gottesman.
    Also available: Print – 1987
  • 2015
    S.S. Gambhir.
    Status: Not Checked OutLane Catalog Record
    Chapter 1. Molecular imaging -- Chapter 2. Basic cell & molecular biology -- Chapter 3. Ovreview of imaging modalities -- Chapter. 4. Molecular imaging strategies -- Chapter 5. Assays -- Chapter 6. Nanoparticle imaging -- Chapter 7. Clinical applications -- Chapter 8. Molecular imaging examples -- Chapter 10. Frequently asked questions -- Chapter 11. Useful tables -- Chapter 12. Probe structures table.
  • 2010 Karger
    volume editor, Karl Lenhard Rudolph.
    Speakers at the Symposium / Hartmann, D. -- Hematopoietic stem cell aging and fate decision / Illing, A., Ju, Z. -- Alterations of systemic environment impair stem cell function during aging / Song, Z., Ju, Z. -- Novel insights into regulation of stem cell aging by aging of the niche and alterations in systemic factors / Geiger, H. -- Aging and hematopoietic malignancies / Gompf, A. -- Intestinal stem cell aging / Sperka, T., Rudolph, K.L. -- Molecular mechanisms of aging in muscle stem cells / Tümpel, S. -- Free radicals and stem cell aging / Guachalla, L.M. -- DNA damage, checkpoint responses, and cell cycle control in aging stem cells / Sperka, T., Nalapareddy, K., Rudolph, K.L.
  • 2016 Springer
    Rafal P. Piprek, editor.
    Early Development of the Gonads -- Origin and Differentiation of the Somatic Cells of the Genital Ridges -- The formation and migration of primordial germ cells in mouse and man -- The Gonadal Supporting Cell Lineage and Mammalian Sex Determination: the Differentiation of Sertoli and Granulosa Cells -- Mesonephric cell migration into the gonads and vascularization are processes crucial for testis development -- Origin and Differentiation of Androgen-producing Cells in the Gonads -- Germ cell commitment to oogenic versus spermatogenic pathway -- the role of retinoic acid -- Ovarian Folliculogenesis -- Control of oocyte growth and development by intercellular communication within the follicular niche -- Biology of the Sertoli cell in the Fetal, Pubertal and Adult Mammalian Testis -- Mechanisms Regulating Spermatogonial Differentiation -- Stem cells in mammalian gonads -- A Role of microRNAs in Cell Differentiation during Gonad Development -- The Battle of the Sexes: Human Sex Development and its Disorders -- Methods for the study of gonadal development.
    Also available: Print – 2016
  • 2013 Springer
    Benjamin Bonavida, editor.
    Patients with various cancers are treated with conventional chemotherapeutic drugs and the majority responds well to such therapies. However, there is a subset of patients who does not respond initially and another subset who no longer responds to further treatments. Clearly, in those two subsets of patients, the cancer cells exhibit mechanisms of resistance. One of the main challenges facing us to date is to develop new therapies to treat patients with the resistant tumors. The development of new effective therapies will be dependent on delineating the biochemical, molecular, and genetic mechanisms that regulate tumor cell resistance. Such mechanisms have revealed gene products that directly regulate resistance and are targets for therapy. Of interest, several FDA-approved drugs were able to overcome drug resistance and have been successfully used clinically. They have been used as monotherapy or synergized with other therapies for the treatment of resistant tumors. This volume constitutes a total of twelve selective reviews by pioneer scientists in the field of cancer drug resistance. Specific mechanisms in drug resistance are reviewed and novel approaches are being proposed for therapeutic interventions. This volume is of general interest to scientists, clinicians, health care providers, and students.
  • 2005 Springer
    S. Milz, M. Benjamin, R. Putz.
  • 2011
    Emily Lawson Egeler.
    Cellular maintenance of protein homeostasis , or proper protein folding, is essential for normal cellular function. Accumulation of misfolded protein can lead to disease and even cell death. The ubiquitin-proteasome system plays a central role in processing cellular proteins for degradation, but little is currently known about how misfolded cytosolic proteins are recognized by protein quality control machinery and targeted for degradation in mammalian cells. In the work presented here, we work with a model protein substrate that is unstable when expressed in cells, but stabilized by addition of a small cell-permeable ligand. Using purified protein, we show that unstable mutants are poorly folded or rapidly sampling unfolded states. The propensity to unfold in vitro correlates with observed instability when expressed in cells. We also show that these substrates are rapidly ubiquitinated en route to degradation at the proteasome in a ligand-dependent manner. We conducted a mass spectrometry screen to identify endogenous protein interactors with the model substrate, which identified a few annotated ubiquitin-proteasome system components. The findings presented herein correlate observed cellular instability to both the propensity of the protein to unfold and the extent of ubiquitination in the ligand-free state. We propose that this protein is a ligand-dependent substrate for the protein quality control machinery, providing an excellent substrate into further query the mechanisms of protein quality control.
  • 2012 Springer
    Domenico Ribatti.
    Introduction -- Morphological aspects of tumor vasculature -- Mechanisms of control of angiogenesis in tumor vasculature -- Anti-Angiogenesis and vascular targeting in tumor vasculature.
  • 2010 Springer
    Lorenzo Alibardi.
  • 2015 Wiley
    Giuseppe d'Onofrio, Gina Zini ; translated by Barbara J. Bain.
    Morphology of Blood Disorders, 2nd edition is an outstanding atlas with over 800 high-quality digital images, covering the whole spectrum of blood and bone marrow morphology, with particular emphasis on malignant haematology. Originally written in the Italian language by two world leaders in the field, the book has been expertly translated by the renowned haematologist and teacher, Barbara Bain. This book explores the major topics of haematological pathology, blending classical teaching with up-to-date WHO classification and terminology. Each image in this book is derived from material obtained for diagnostic purposes from patients with serious haematological conditions. Morphological details are supplemented by detailed descriptions of the output and role of automated instruments in disorders of the blood. Morphology of Blood Disorders, 2nd edition is an essential reference source for diagnosis in the haematology laboratory, designed to be the go-to guide for anyone with an interest in blood cell morphology.
  • 2012 Springer
    Sunil C. Kaul, Renu Wadhwa, editors.
    Part 1. Structure and Function of Mortalin / Birth of Mortalin: Multiple Names, Niches and Functions Connecting Stress, Senescence and Cancer / Renu Wadhwa and Sunil C. Kaul -- Mortalin's Machinery / Custer C. Deocaris, Sunil C. Kaul and Renu Wadhwa -- The Role of Mortalin in Iron Homeostasis / Wen-I Luo and James A. Cowan -- Functional Characteristics of Mortalin / Walter A. Baseler, Tara L. Croston and John M. Hollander -- Part 2. Mortalin in Evolution2 Deocaris / Mortalin and Drosophila DmHsp22: Two Mitochondrial Chaperones Regulating Aging and Carcinogenesis / Marie Le Pécheur, Geneviève Morrow and Robert M. Tanguay -- Mortalin in Invertebrates and The Induction of Apoptosis by Wild-Type p53 Following Defeat of Mortalin-Based Cytoplasmic Sequestration in Cancerous Clam Hemocytes / Charles W. Walker, Ben Low and S. Anne Böttger -- Mortalin and Stem Cells: A Study from Planarians / Renata Batistoni -- Part 3. Mortalin in Health and Disease / Mortalin in Cell Protection from Immune Attack / Moran Saar, Oren Moskovich and Zvi Fishelson -- Mortalin in Neurological Diseases / Jinghua Jin, Jing Zhang, Travis J. Cook and Jake G. Hoekstra -- Loss of Mortalin Function in Parkinson's Disease-Supporting the Mitochondrial Pathway of Neurodegeneration / Lena F. Burbulla and Rejko Krüger -- Hsp75/mortalin and Protection from Ischemic Brain Injury / Robin E. White, Yi-Bing Ouyang and Rona G. Giffard -- Catecholamine Regulated Protein (CRP40), A Splice Variant of Mortalin-2: Functional Role in CNS Disorders / Joseph P. Gabriele, Sarah E. Groleau, Ritesh P. Daya, Zdenek B. Pristupa and Ram K. Mishra -- Chaperonopathies: Diseases in Which Mortalin and Other Hsp-Chaperones Play a Role in Etiology and Pathogenesis / Alberto J. L. Macario, Francesco Cappello and Everly Conway de Macario -- Part 4. Mortalin and Cancer / Many Faces of Mortalin and Tid1 / Ohad Iosefson and Abdussalam Azem -- Mortalin: A Positive Regulator of Centrosome Duplication and Amplification / Masayuki Kanai and Kenji Fukasawa -- Mortalin Expression in Normal and Neoplastic Tissues / Angheliki Nomikos, Sinclair R. Dundas and Graeme I. Murray -- Mortalin-p53 Interaction as a Target for Liver Cancer Therapy / Wen-Jing Lu, Nikki P. Lee, Renu Wadhwa and John M. Luk -- Mortalin Targeting Gadgets for Cancer Therapy / Chae-Ok Yun and Renu Wadhwa -- Part 5. Mortalin Based Technologies / Cell Internalizing Anti-Mortalin Antibody for Generation of Illuminating MSCs for Long-Term In vitro and In vivo Tracking / Toshimasa Uemura, Masanori Nishi, Sunil C. Kaul and Renu Wadhwa -- Mortalin Staining Pattern as a Reporter for Cell Based Anti-Cancer Drug Screening / Ran Gao, Zeenia Kaul, Tomoko Yaguchi and Renu Wadhwa -- Cell Internalizing Anti-mortalin Antibody as a Nanocarrier / Zeenia Kaul, Tomoko Yaguchi, Renu Wadhwa and Sunil C. Kaul.
  • 2012 Springer
    Jacek Z. Kubiak, editor.
    Post-transcriptional Control of Gene Expression During Mouse Oogenesis / Hugh J. Clarke -- Polarity and Asymmetry During Mouse Oogenesis and Oocyte Maturation / Malgorzata Kloc, Rafik M. Ghobrial, Ewa Borsuk and Jacek Z. Kubiak -- Chromatin Structure and ATRX Function in Mouse Oocytes / Rabindranath De La Fuente, Claudia Baumann and Maria M. Viveiros -- Cyclin B in Mouse Oocytes and Embryos: Importance for Human Reproduction and Aneuploidy / Zbigniew Polański, Hayden Homer and Jacek Z. Kubiak -- Src Protein Kinases in Mouse and Rat Oocytes and Embryos / Mattan Levi, Lihi Ninio-Mani and Ruth Shalgi -- Gradual Meiosis-To-Mitosis Transition in the Early Mouse Embryo / Aurélien Courtois and Takashi Hiiragi -- Maternal Control of Mouse Preimplantation Development / Wenjing Zheng and Kui Liu -- Preimplantation Mouse Embryo: Developmental Fate and Potency of Blastomeres / Aneta Suwińska -- Creation of Trophectoderm, the First Epithelium, in Mouse Preimplantation Development / Yusuke Marikawa and Vernadeth B. Alarcon -- Cell Lineage Allocation Within the Inner Cell Mass of the Mouse Blastocyst / Panagiotis Xenopoulos, Minjung Kang and Anna-Katerina Hadjantonakis -- Formation of Distinct Cell Types in the Mouse Blastocyst / Samantha A. Morris and Magdalena Zernicka-Goetz -- Cell Movements in the Egg Cylinder Stage Mouse Embryo / Bradley Joyce and Shankar Srinivas -- Balancing the Dose in the Mouse / Mary E. Donohoe -- Mouse Oviduct Development / C. Allison Stewart and Richard R. Behringer -- Cell Lineages, Growth and Repair of the Mouse Heart / Fabienne Lescroart and Sigolène M. Meilhac -- Cellular Reprogramming During Mouse Development / Zoë D. Burke, Gabriela Miron-Buchacra and David Tosh -- Differentiation of Definitive Endoderm from Mouse Embryonic Stem Cells / Peter T. W. Kim and Christopher J. Ong -- Mouse and Human Pluripotent Stem Cells and the Means of Their Myogenic Differentiation / I. Grabowska, K. Archacka, A. M. Czerwinska, M. Krupa and M. A. Ciemerych -- Stem Cells and Corneal Epithelial Maintenance: Insights from the Mouse and Other Animal Models / Richard L. Mort, Panagiotis Douvaras, Steven D. Morley, Natalie Dorà and Robert E. Hill, et al. -- Mouse-Induced Pluripotent Stem Cells / Hui Zhang and Ying Jin -- Aging in the Mouse and Perspectives of Rejuvenation Through Induced Pluripotent Stem Cells (iPSCs) / Ken-ichi Isobe, Zhao Cheng, Sachiko Ito and Naomi Nishio.
    Also available: Print – 2012
  • 2014 Springer Protocols
    edited by Mark Lewandoski.
    In situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional [beta]-galactosidase Staining -- Two Color In Situ Hybridization of Whole-Mount Mouse Embryos -- Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections -- Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays -- EMAGE: Electronic Mouse Atlas of Gene Expression -- Real-time PCR Quantification of gene Expression in Embryonic Mouse Tissue -- Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic Approach -- Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene Expression -- Generation of Tissue Organoids by Compaction Reaggregation -- Ultra-Rapid Vitrification of Mouse Oocytes and Embryos -- Mammalian Preimplantation Embryo Culture -- Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs -- Genetically Encoded Probes Provide a Window On Embryonic Arrhythmia -- Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model Organisms -- Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse Embryos -- Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues -- Protein/Peptide Transduction in Metanephric Explant Culture -- Direction of Cells Programmed to Die in Mouse Embroys -- Microscopic Computed Tomography-based ?Virtual Histology of Embryos -- Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study -- The Latest Improvements in the Mouse Sperm Preservation -- Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitro -- Using the Textpresso Site Specific Recombinases Web Server to Identify ?Cre Expressing Mouse Strains and Floxed Alleles -- Live Imaging Mouse Embryonic Development: Seeing is Believing and Revealing -- Genetic Cell Ablation -- Essentials of Recombinase-based Genetic Fate Mapping in Mice.
  • 2012 Springer Protocols
    edited by Thomas Weichhart.
    Mammalian target of rapamycin : a signaling kinase for every aspect of cellular life / Thomas Weichhart -- Biochemical and pharmacological inhibition of mTOR by rapamycin and an ATP-competitive mTOR inhibitor / Ker Yu and Lourdes Toral-Barza -- Evaluation of the nutrient-sensing mTOR pathway / Sungki Hong, Aristotle M. Mannan, and Ken Inoki -- mTor activity under hypoxia / Douangsone D. Vadysirisack and Leif W. Ellisen -- Isolation of the mTOR complexes by affinity purification / Dos D. Sarbassov [and others] -- An in vitro assay for the kinase activity of mTOR complex 2 / Jingxiang Huang -- Overexpression or downregulation of mTOR in mammalian cells / Mahmoud Khalil and Ivan Gout -- Detection of cytoplasmic and nuclear functions of mTOR by fractionation / Margit Rosner and Markus Hengstschläger -- Evaluation of rapamycin-induced cell death / Lorenzo Galluzzi [and others] -- Evaluation of mTOR-regulated mRNA translation / Valentina Iadevaia [and others] -- A genome-wide RNAi screen for polypeptides that alter rpS6 phosphorylation / Angela Papageorgiou and Joseph Avruch -- Immunohistochemical analysis of mTOR activity in tissues / Jinhee Kim [and others] -- Assessing cell size and cell cycle regulation in cells with altered TOR activity / Megan Cully and Julian Downward -- Quantitative visualization of autophagy induction by mTOR inhibitors / Beat Nyfeler [and others] -- The in vivo evaluation of active-site TOR inhibitors in models of BCR-ABL+ leukemia / Matthew R. Janes and David A. Fruman -- Inducible raptor and rictor knockout mouse embryonic fibroblasts / Nadine Cybulski, Vittoria Zinzalla, and Michael N. Hall -- Expanding human T regulatory cells with the mTOR-inhibitor rapamycin / Manuela Battaglia, Angela Stabilini, and Eleonora Tresoldi -- Rapamycin-induced enhancement of vaccine efficacy in mice / Chinnaswamy Jagannath and Pearl Bakhru -- Utilizing a retroviral RNAi system to investigate in vivo mTOR functions in T cells / Koichi Araki and Bogumila T. Konieczny -- Exploring functional in vivo consequences of the selective genetic ablation of mTOR signaling in T helper lymphocytes / Greg M. Delgoffe and Jonathan D. Powell -- Evaluating the therapeutic potential of mTOR inhibitors using mouse genetics / Huawei Li, Jennifer L. Cotton, and David A. Guertin -- Inhibition of PI3K-Akt-mTOR signaling in glioblastoma by mTORC1/2 inhibitors / Qi-Wen Fan and William A. Weiss -- Assessing the function of mTOR in human embryonic stem cells / Jiaxi Zhou, Dong Li, and Fei Wang -- Video-EEG monitoring methods for characterizing rodent models of tuberous sclerosis and epilepsy / Nicholas R. Rensing, Dongjun Guo, and Michael Wong -- A genetic model to dissect the role of TSC-mTORC1 in neuronal cultures / Duyu Nie and Mustafa Sahin -- Tissue-specific ablation of TSC1 in pancreatic beta-cells / Hiroyuki Mori and Kun-Liang Guan -- A mouse model of diet-induced obesity and insulin resistance / Chao-Yung Wang and James K. Liao -- Rapamycin as immunosuppressant in murine transplantation model / Louis-Marie Charbonnier and Alain Le Moine -- Development of ATP-competitive mTOR inhibitors / Qingsong Liu [and others].
  • 2012 Springer
    Allison D. Fryer, Arthur Christopoulos, Neil M. Nathanson, editors.
    Muscarinic receptor pharmacology and signaling -- Overview of muscarinic receptor subtypes -- Structure-function studies of muscarinic acetylcholine receptors -- Polymorphisms in human muscarinic receptor subtype genes -- Muscarinic receptor trafficking -- Physiological role of G-protein coupled receptor phosphorylation -- Novel muscarinic receptor mutant mouse models -- Muscarinic receptors in the CNS -- Muscarinic receptor pharmacology and circuitry for the modulation of cognition -- Muscarinic agonists and antagonists in schizophrenia -- Muscarinic pain pharmacology: realizing the promise of novel anagesics by overcoming old challenges -- Muscarinic modulation of striatal function and circuitry -- Muscarinic receptors in brain stem and mesopontine cholinergic arousal functions -- Muscarinic receptors in autonomic effector organs -- Muscarinic receptor agonists and antagonists: effects on ocular function -- Muscarinic receptor agonists and antagonists: effects on cardiovascular function -- Muscarinic receptor antagonists: effects on pulmonary function -- Muscarinic agonists and antagonists: effects on gastrointestinal function -- Muscarinic agonists and antagonists: effets on the urinary bladder -- Muscarinic receptors and mediation of hormonal effects of acetylcholine -- Muscarinic receptor agonists and antagonists: effects on inflammation and immunity -- Muscarinic receptor agonists and antagonists: effects on keratinocyte functions -- Muscarinic receptor agonists and antagonists: effects on cancer -- Activation of muscarinic receptors by non-neuronal acetylcholine.
  • 2006 Springer
    [edited by] Helen Sink.
    An introduction to muscle development in drosophila / Helen Sink -- The muscle pattern of drosophila / Volker Hartenstein -- Mesoderm formation in the drosophila embryo / Noriko Wakabayashi-Ito and Y. Tony Ip -- Development of the cardiac musculature / Rolf Bodmer -- Development of the somatic gonad and fat bodies / Mark Van Doren -- Development of the larval visceral musculature / Hsiu-Hsiang Lee, Stephane Zaffran and Manfred Frasch -- Development of the larval somatic musculature / Ana Carmena and Mary Baylies -- Muscle morphogenesis: the process of embryonic myoblast fusion / Susan M. Abmayr and Kiranmai S. Kocherlakota -- Muscle attachment sites-where migrating muscles meet their match / Talila Volk -- Neuromuscular development: connectivity and plasticity / Louise Nicholson and Haig Keshishian -- Metamorphosis and the formation of the adult musculature / Devkanya Dutta and K. VijayRaghavan -- Molecular basis of muscle structure / Jim O. Vigoreaux -- Whole genome approaches to studying drosophila muscle development / Eileen E.M. Furlong -- Comparison of muscle development in drosophila and vertebrates / Michael V. Taylor.
  • 2014 Springer
    Swati Palit Deb, Sumitra Deb, editor.
    This book provides the readers with an overview of research on p53, which has been shown to play a role in numerous crucial biological pathways in normal and cancer cells. Leading scientist in the field, who have all made direct contributions to the understanding of the molecular events underpinning p53 function, have been invited to contribute the various chapters, which discuss the current knowledge of the signaling cascades that are activated by mutations in p53 and overexpression of MDM2, frequently found in human cancer, and are major causes of oncogenesis. This book features chapters on the molecular basis of oncogenesis induced by gain of function mutation of p53, signaling pathways induced by MDM2 overexpression, control of mutant or wild-type p53 function by MDM2 and MDMX, p53 mutation in hereditary cancer, and structural aspects that activate mutant p53 which can be targeted by drug therapy. This book should be useful for scientists at all levels.
  • 2013 Springer Protocols
    edited by Laura Soucek, Nicole M. Sodir.
  • 2008 Springer Protocols
    edited by Tanya Parish and Amanda Claire Brown.
    Isolation of Mycobacterium species genomic DNA / John T. Belisle, Spencer B. Mahaffey, and Preston J. Hill -- Isolation of mycobacterial RNA / Tige R. Rustad [and others] -- Lipoglycans of Mycobacterium tuberculosis : isolation, purification, and characterization / Libin Shi, Jordi B. Torrelles, and Delphi Chatterjee -- Analyzing lipid metabolism : activation and [beta]-oxidation of fatty acids / Paul Robert Wheeler -- Whole genome analysis using microarrays / S.J. Waddell, J. Hinds, and P.D. Butcher -- Use of DNA microarrays to study global patterns of gene expression / Roberta Provvedi, Giorgio Palù, and Riccardo Manganelli -- Two-dimensional gel electrophoresis-based proteomics of mycobacteria / Jens Mattow [and others] -- Transport assays and permeability in pathogenic mycobacteria / Marie-Antoinette Lanéelle and Mamadou Daffé -- Continuous culture of mycobacteria / Joanna Bacon and Kim A. Hatch -- Measuring minimum inhibitory concentrations in mycobacteria / Frederick A. Sirgel, Ian J.F. Wiid, and Paul D. van Helden -- Rapid screening of inhibitors of Mycobacterium tuberculosis growth using tetrazolium salts / Anita G. Amin [and others] -- Electroporation of mycobacteria / Renan Goude and Tanya Parish -- Ins and outs of mycobacterial plasmids / Farahnaz Movahedzadeh and Wilbert Bitter -- The use of temperature-sensitive plasmids in mycobacteria / Damien Portevin, Wladimir Malaga, and Christophe Guilhot -- Heterologous expression of genes in mycobacteria / James A. Triccas and Anthony A. Ryan -- Inducible expression systems for mycobacteria / Christopher M. Sassetti -- Assaying promoter activity using LacZ and GFP as reporters / Paul Carroll and Jade James -- Construction of unmarked deletion mutants in mycobacteria / Houhui Song, Frank Wolschendorf, and Michael Niederweis -- Construction of targeted mycobacterial mutants by homologous recombination / Sharon L. Kendall and Rosangela Frita -- Phage transposon mutagenesis / M. Sloan Siegrist and Eric J. Rubin -- Gene essentiality testing in Mycobacterium smegmatis using specialized transduction / Apoorva Bhatt and William R. Jacobs, Jr. -- Gene switching and essentiality testing / Amanda Claire Brown -- Insertion element IS6110-based restriction fragment length polymorphism genotyping of Mycobacterium tuberculosis / Robin M. Warren, Paul D. van Helden, and Nicolaas C. Gey van Pittius -- Typing Mycobacterium tuberculosis using variable number tandem repeat analysis / T.J. Brown, V.N. Nikolayevskyy, and F.A. Drobniewski -- Molecular detection of drug-resistant Mycobacterium tuberculosis with a scanning-frame oligonucleotide microarray / Dmitriy V. Volokhov [and others] -- MycoDB : an online database for comparative genomics of the mycobacteria and related organisms / Roy R. Chaudhuri -- Environmental amoebae and mycobacterial pathogenesis / Melanie Harriff and Luiz E. Bermudez.
  • 2012 Springer Protocols
    edited by Joseph X. DiMario.
    Isolation and characterization of human fetal myoblasts/ Ariya D. Lapan and Manuela Gussoni -- Skeletal muscle satellite cells : background and methods for isolation and analysis in a primary culture system / Maria Elena Danoviz and Zipora Yablonka-Reuveni -- Isolation of muscle stem cells by fluorescence activated cell sorting cytometry / Alessandra Pasut, Paul Oleynik, and Michael A. Rudnicki -- Mouse and human mesoangioblasts : isolation and characterization from adult skeletal muscles / Mattia Quattrocelli [and others] -- Direct electrical stimulation of myogenic cultures for analysis of muscle fiber type control / Eric J. Cavanaugh, Jennifer R. Crew, and Joseph X. DiMario -- Single muscle-fiber isolation and culture for cellular, molecular, pharmacological, and evolutionary studies / Judy E. Anderson, Ashley C. Wozniak, and Wataru Mizunoya -- Somite unit chronometry to analyze teratogen phase specificity in the paraxial mesoderm / Sarah J. Venters and Charles P. Ordahl -- Analysis of skeletal muscle development in drosophila / Ginny R. Morriss [and others] -- Immunocytochemistry to study myogenesis in zebrafish / Nathan C. Bird, Stefanie E. Windner, and Stephen H. Devoto -- Immunofluorescent localization of proteins in Caenorhabditis elegans muscle / Kristy J. Wilson, Hiroshi Qadota, and Guy M. Benian -- Resistance loading and signaling assays for oxidative stress in rodent skeletal muscle / Stephen E. Alway and Robert G. Cutlip -- Analysis of skeletal muscle hypertrophy in models of increased loading / Sue C. Bodine and Keith Baar -- Protein overexpression in skeletal muscle using plasmid-based gene transfer to elucidate mechanisms controlling fiber size / Chia-Ling Wu and Susan C. Kandarian -- In vivo measurement of muscle protein synthesis rate using the flooding dose technique / Marta L. Fiorotto, Horacio A. Sosa Jr., and Teresa A. Davis -- Recombinant adeno-associated viral vector production and purification / Jin-Hong Shin, Yongping Yue, and Dongsheng Duan -- Generation of lentiviral vectors for use in skeletal muscle research / Christophe Pichavant and Jacques P. Tremblay -- Generating tamoxifen-inducible Cre alleles to investigate myogenesis in mice / Christoph Lepper and Chen-Ming Fan -- Gene profiling studies in skeletal muscle by quantitative real-time polymerase chain reaction assay / Shephali Bhatnagar, Siva K. Panguluri, and Ashok Kumar -- Analysis of lipid profiles in skeletal muscles / Vassilis Mougios and Anatoli Petridou -- Proteomic analysis of dystrophic muscle / Caroline Lewis, Philip Doran, and Kay Ohlendieck -- Detection of calcium release via ryanodine receptors / Jerry P. Eu and Gerhard Meissner -- Measurement of calcium release due to inositol trisphosphate receptors in skeletal muscle / Mariana Casas, Francisco Altamirano, and Enrique Jaimovich -- Detection of calcium sparks in intact and permeabilized skeletal muscle fibers / Noah Weisleder, Jingsong Zhou, and Jianjie Ma -- Analysis of calcium transients in cardiac myocytes and assessment of the sarcoplasmic reticulum Ca(2+)-ATPase contribution / Anand Mohan Prasad and Giuseppe Inesi -- Analysis of muscle gene transcription in cultured skeletal muscle cells / Charis L. Himeda, Phillip W.L. Tai, and Stephen D. Hauschka -- Analysis of fiber-type differences in reporter gene expression of [beta]-gal transgenic muscle / Phillip W.L. Tai [and others] -- Determination of gene promoter activity in skeletal muscles in vivo / Sarah M. Senf and Andrew R. Judge -- Determination of miRNA targets in skeletal muscle cells / Zhan-Peng Huang, Ramón Espinoza-Lewis, and Da-Zhi Wang -- ShRNA-mediated gene knockdown in skeletal muscle / Muriel Golzio, Jean-Michel Escoffre, and Justin Teissié -- Detection of NF-[kappa]B activity in skeletal muscle cells by electrophoretic mobility shift analysis / Jason M. Dahlman and Denis C. Guttridge -- Isolation of nuclei from skeletal muscle satellite cells and myofibers for use in chromatin immunoprecipitation assays / Yasuyuki Ohkawa [and others] -- An improved restriction enzyme accessibility assay for analyzing changes in chromatin structure in samples of limited cell number / Yasuyuki Ohkawa [and others] -- ChIP-enriched in silico targets (ChEST), a ChIP-on-chip approach applied to analyzing skeletal muscle genes / Guillaume Junion and Krzysztof Jagla.
  • 2014 CRCnetBASE
    edited by Bhanu P. Jena and Douglas J. Taatjes.
    Porosomes--the universal secretory portals in cells: a brief essay / Bhanu P. Jena -- The hair cell porosome: molecular and synaptic implications / Dennis G. Drescher -- The neuronal porosome complex in mammalian brain: a study using electron microscopy / Mzia G. Zhvania, Nadezhda J. Japaridze, Mariam G. Qsovreli, Vera G. Okuneva, Arkadi G. Surmava, and Tamar G. Lordkipanidze -- Granule size distribution suggests mechanism: the case for granule growth and elimination as a fusion nano-machine / Ilan Hammel and Isaac Meilijson -- Probing protein assembly, biomineralization, and biomolecular interactions by atomic force microscopy / Kang Rae Cho and James J. De Yoreo -- High-resolution imaging of amylin aggregation and internalization in pancreatic cells: implications in health and disease / Saurabh Trikha, Sanghamitra Singh, and Aleksandar M. Jeremic -- Repair of Nanodefects in a two-dimensional crystal anticoagulant shield in the antiphospholipid syndrome: novel molecular strategies assessed by atomic force microscopy / Douglas J. Taatjes, Anthony S. Quinn, Xiao-Xuan Wu, Han-Mou Tsai, and Jacob H. Rand -- A novel approach to study molecular evolution: detection of ancestral conformation hidden in present-day proteins using antibody as nanostructure probes / Jian-Ping Jin -- mRNA nanomachines and stress reprogramming following brain ischemia / Donald J. DeGracia, Jill T. Jamison, Manupreet Chawla, Monique K. Lewis, Michelle Smith, and Jeffery J. Szymanski -- Physical properties and biomedical applications of superparamagnetic iron oxide nanoparticles / Gavin Lawes, Ratna Naik, and Prem Vaishnava -- Atomic force microscopy imaging of DNA delivery nanosystems / Yi Zou, David Oupicky, and Guangzhao Mao -- Impedance spectroscopy for characterization of biological function / Brian C. Riggs, Janet L. Paluh, G.E. Plopper, and Douglas B. Chrisey.
  • 2014 Springer
    David G. Capco, Yongsheng Chen, editors.
    Presence in, and release of, nanomaterials from consumer products / Yu Yang and Paul Westerhoff -- Nanoparticle aggregation: principles and modeling / Wen Zhang -- Influences of nanomaterials on the barrier function of epithelial cells / Shariq Ali and Erik Rytting -- Engineered nanoparticles induced brush border disruption in a human model of the intestinal epithelium / James J. Faust, Benjamin M. Masserano, Adam H. Mielke, Anup Abraham, and David G. Capco -- Nanoparticles: cellular uptake and cytotoxicity / Isaac M. Adjei, Blanka Sharma and Vinod Labhasetwar -- Atomic force microscopy study of the interaction of DNA and nanoparticles / Kungang LI, Songyan Du, Steven Van Ginkel, and Yongsheng Chen -- Intracellular signal modulation by nanomaterials / Salik Hussain, Stavros Garantziotis, Fernando Rodrigues-Lima, Jean-Marie Dupret, Armelle Baeza-Squiban, Sonja Boland -- Nanomaterials: impacts on cells and cell organelles / Željka Krpetić, Sergio Anguissola, David Garry, Philip Kelly and Kenneth A. Dawson -- Design, synthesis, and functionalization of nanoparticles for therapeutic drug delivery / Taraka Sai Pavan Grandhi and Kaushal Rege -- Preparation of nanoscale pulmonary drug delivery formulations by spray drying / Adam Bohr, Christian A. Ruge, and Moritz Beck-Broichsitter -- Nanomedicine: the promise and challenges in cancer chemotherapy / Youssef W. Naguib and Zhengrong Cui -- Transgenerational effects of NMs / Anna Poma, Sabrina Colafarina, Gabriella Fontecchio, and Giuseppe Chichiricco -- Stem cells and nanomaterials / Maria-Claude Hofmann.
    Also available: Print – 2014
  • 2010 Springer
    Jacques Zimmer, editor.
  • 2010 ScienceDirect
    editors, Michael T. Lotze, Angus W. Thomson.
    Chapter 1, Developmental stages and pathways of NK cell maturation / Bartosz Grzywacz, Jeffery S. Miller, Michael R. Verneris -- Chapter 2. Developmental origins of thymus-derived natural killer cells / James R. Carlyle, Juan C. Zúñiga-Pflücker -- Chapter 3. Interferon-producing killer dendritic cells (IKDC) / Franck Housseau -- Chapter 4. Natural killer cells in cancer / Petter Höglund, Hans-Gustaf Ljunggren -- Chapter 5. NK cell immune recognition: NKG2D ligands and stressed cells / Carlos López-Larrea, Alejandro López-Soto, Segundo González -- Chapter 6. NK cell KIR heterogeneity and evolution / Makoto Yawata, Nobuyo Yawata -- Chapter 7. Signalling events in natural killer cells / P.K. Epling-Burnette, Sheng Wei, Julie Y. Djeu -- Chapter 8. Human natural killer cell development / Edward L. Briercheck, Aharon G. Freud, Michael A. Caligiuri -- Chapter 9. Isolation, culture and propagation of natural killer cells / Michael Magee, Lazar N. Vujanovic, Lisa H. Butterfield, Nikola L. Vujanovic -- Chapter 10. The localization and migration of natural killer cells in health and disease / Vasileios Bekiaris, Peter J.L. Lane -- Chapter 11. Receptors on NK cells / Noam Stern-Ginossar, Ofer Mandelboim -- Chapter 12. Development and testing of NK cell lines / Hans Klingemann -- Chapter 13. NK cell-derived cytokines and delivery: NK cell synapses / Claudia Semino, Anna Rubartelli -- Chapter 14. NK cells as recipients of cytokine signals / Tara J. Loux, Michael T. Lotze, Herbert J. Zeh III -- Chapter 15. NK cells and chemokines / Angela Gismondi, Giovanni Bernardini, Angela Santoni -- Chapter 16. Natural killer cytolytic activity / Lesley R. de Armas, Eckhard R. Podack -- Chapter 17. Illuminating intranodal natural killer cell behaviour using two-photon microscopy / Kym R. Garrod, Michael D. Cahalan -- Chapter 18. Dendritic cells and NK cells / Amy K. Wesa, Robbie B. Mailliard -- Chapter 19. NK and NKT cells: The innate-adaptive interface including humoral responses / Jeff Subleski, Jonathan M. Weiss, Robert H. Wiltrout, John R. Ortaldo -- Chapter 20. NK cell and endothelial cell interactions / Angela Gismondi, Claudia Carlino, Helena Stabile, Angela Santoni -- Chapter 21. Natural killer cell/epithelial interactions / Taisuke Ito, Masao Takei, Ralf Paus -- Chapter 22. NK cell-T cell interactions / Benedict J. Chambers -- Chapter 23. NK cells and NKT cells in cutaneous disorders / Anna Balato, Anthony A. Gaspari -- Chapter 24. Natural killer cells in the respiratory tract / Borna Mehrad -- Chapter 25. Intestinal natural killer cells / Martin R. Goodier -- Chapter 26. Natural killer cells in the liver / Shiguang Qian, Jia Fan, Shuang-Jian Qiu, Yong-sheng Xiao, Lina Lu -- Chapter 27. Natural killer cells in the spleen and lymph node / Alex Karlsson-Parra -- Chapter 28. Nature killer cells in the central nervous system / Fu-Dong Shi, Richard M. Ransohoff -- Chapter 29. NK cells in the eye / Jerry Y. Niederkorn -- Chapter 30. NK cells and reproduction / Ashley Moffett, Victoria H. Male -- Chapter 31. Identification of natural killer cells in tissues and their isolation / Paolo Carrega, Erika Montalto, Guido Ferlazzo -- Chapter 32. Natural killer cell immune regulation: Coordination of immune function in tissues / Lorenzo Moretta, Alessandro Moretta -- Chapter 33. Natural killer cells and transplantation / Benjamin M. Matta, Angus W. Thomson -- Chapter 34. Natural killer cells and autoimmunity / Christian Münz -- Chapter 35. Interaction of NK cells with bacteria / Kenji Chamoto, Daiko Wakita, Shinichi Koizumi, Kazutaka Masuko, Takayuki Ikeda, Rieko Mitamura, Takashi Nishimura -- Chapter 36. Natural killer cells and human immunodeficiency virus / Domenico Mavilio, Anne Hosmalin, Daniel Scott-Algara -- Chapter 37. Cytomegalovirus infection and NK cells / Jerome D. Coudert, Christopher E. Andoniou, Mariapia A. Degli-Esposti -- Chapter 38. Natural killer cells in allergy / Syh-Jae Lin, Yen-Chang Lee, Dah-Chin Yan, Ming-Ling Kuo -- Chapter 39. Natural killer cells in wound healing / Jussi Liippo, Mervi Toriseva, Veli-Matti Kähäri -- Chapter 40. Natural killer cells in atherosclerosis / Godfrey S. Getz, Catherine A. Reardon -- Chapter 41. Natural killer cells in leukaemia / Jérôme Rey, Cyril Fauriat, Alessandro Moretta, Régis Costello, Daniel Olive -- Chapter 42. Natural killer cells and allogeneic haematopoietic cell transplantation / Andrea Velardi -- Chapter 43. Clinical trials of NK cells for cancer / Sarah Cooley, Jeffrey S. Miller -- Chapter 44. Natural killer cells and hepatitis C virus infection / Michael A. Nalesnik, Tatsuya Kanto -- Chapter 45. Natural killer cells and the biology of parasitism / Marianne A. Bryan, Karen A. Norris -- Chapter 46. Lymphoproliferative disorders of natural killer cells / William G. Morice -- Chapter 47. Natural killer cell induction of tolerance / Lina Lu, Alexandra Y. Zhang, William L. Camp, Shiguang Qian -- Chapter 48. Genetic engineering of natural killer cells / Stephen R. Goding, Per H. Basse -- Chapter 49. NK cell-mediated target cell death / Beverly Z. Packard, Akira Komoriya, David S. Ucker -- Chapter 50. NK-cell tracking using non-invasive imaging modalities / Priyanka Jha, Verena Reinhart, Christiane Knopp, Siyuan Liu, Winfried S. Wels, Michael F. Wendland, Heike Daldrup-Link.
  • 2012 Springer
    Masaki Terabe, Jay A. Berzofsky, editors.
    Introduction : mechanisms of NKT-cell-mediated adjuvant activity and function of iPS-derived NKT cells / Masaru Taniguchi ... [et al.] -- Structure and recognition of antigens for invariant NKT cells / Bo Pei and Mitchell Kronenberg -- Invariant NKT cell-based vaccine strategies / John-Paul Jukes ... [et al.] -- Immune regulation of tumor immunity by NKT cells / Jessica J. O'Konek, Jay A. Berzofsky, and Masaki Terabe -- The regulation of CD1d+ and CD1d- tumors by NKT cells : the roles of NKT cells in regulating CD1d+ and CD1d- tumor immunity / Jianyun Liu, Gourapura J. Renukaradhya, and Randy R. Brutkiewicz -- DC-based immunotherapy targeting NKT cells / Shin-ichiro Fujii and Kanako Shimizu -- Therapeutic approaches utilising NKT cells / Stephen R. Mattarollo and Mark J. Smyth -- NKT cells or cancer patients and how models can inform therapeutic plans / Mark A. Exley, Lydia Lynch, and Michael Nowak -- Understanding the role of natural killer T cells in hematologic malignancies : progress and challenges / Natalia Neparidze and Madhav V. Dhodapkar -- Clinical trials with alpha-galactosylceramide (KRN7000) in advanced cancer / Famke L. Schneiders ... [et al.] -- Clinical trials of invariant natural killer T cell-based immunotherapy for cancer / Shinichiro Motohashi, Yoshitaka Okamoto, and Toshinori Nakayama.
  • 2013 Springer Protocols
    edited by Kimberly McCall, Charles Klein.
    Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. -- Publisher website.
  • 2014 ScienceDirect
    edited by Paul A. Trainor.
    This book summarizes discoveries of historical significance and provides in-depth, current analyses of the evolution of neural crest cells, their contribution to embryo development, and their roles in disease. In addition, prospects for tissue engineering, repair and regeneration are covered, offering a timely synthesis of the current knowledge in neural crest cell research.
  • 2006 Springer
    edited by Mahendra S. Rao.
  • 2012 Springer
    Mahendra S. Rao, Melissa Carpenter, Mohan C. Vemuri, editors.
    Defining neural stem cells and their role in normal development of the nervous system / Sally Temple -- Embryonic stem cells and neurogenesis / Robin L. Wesselschmidt -- The cellular and molecular properties of multipotent neural stem cells / Scott R. Hutton and Larysa H. Pevny -- Multipotent stem cells in the embryonic nervous system / Chian-Yu Peng ... [et al.] -- Neural crest formation and diversification / Marcos S. Simões-Costa ... [et al.] -- Glial nature of adult neural stem cells : neurogenic competence in adult astrocytes / William W. Hwang, Arturo Alverez-Buylla and Daniel A. Lim -- Neural stem cell death regulation in nervous system development and disease / K.C. Walls and Kevin A. Roth -- Stem cells of adult olfactory epithelium / James E. Schwob, Woochan Jang and Eric H. Holbrook -- Retinal stem cells / Carolina B. Del Debbio ... [et al.] -- Transdifferentiation in the nervous system / Ying Liu and Mahendra S. Rao -- Neural and dopaminergic differentiation of human pluripotent stem cells / Olga Momcilovic and Xianmin Zeng -- Mobilization of neural stem cells in the adult central nervous system / Harish Babu, Theo D. Palmer and Fred H. Gage -- Stem cell pathways in brain tumors / Justin D. Lathia ... [et al.].
  • 2013 Springer Protocols
    edited by Brent A. Reynolds, Loic P. Deleyrolle.
    Culturing Fetal Precursor Cells Using Free Floating Serum-Free Conditions / Andrew Chojnacki and Samuel Weiss -- In Vitro Expansion of Fetal Neural Progenitors as Adherent Cell Lines / Steven M. Pollard -- Isolate and Culture Precursor Cells from the Adult Periventricular Area / Chiara Cavazzin, Margherita Neri, and Angela Gritti -- Culturing and Expansion of Precursor Cells from the Adult Hippocampus / Dhanisha J. Jhaveri, Boris W. Prosper, and Perry F. Bartlett -- Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord / Jean-Philippe Hugnot -- Culturing and Expansion of "Clinical Grade" Precursors Cells from the Fetal Human Central Nervous System / Maurizio Gelati [and five others] -- Isolating and Culturing of Precursor Cells from the Adult Human Brain / Florian A. Siebzehnrubl and Dennis A. Steindler -- Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord / Luc Bauchet [and five others] -- Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny / Hassan Azari -- Isolation of Adult Stem Cells from the Human Olfactory Mucosa / François Féron [and three others] -- Enumerating Stem Cell Frequency: Neural Colony Forming Cell Assay / Sharon A. Louis and Carmen K.H. Mak -- Flow Cytometry of Neural Cells / Geoffrey W. Osborne -- Neonatal Transplant in Hypoxic Injury / Tong Zheng and Michael D. Weiss -- Isolation and Purification of Self-Renewable Human Neural Stem Cells for Cell Therapy in Experimental Model of Ischemic Stroke / Ricardo L. Azevedo-Pereira and Marcel M. Daadi -- Transplantation of Fetal Midbrain Dopamine Progenitors into a Rodent Model of Parkinson's Disease / Lachlan H. Thompson and Clare L. Parish -- Distribution of Neural Precursor Cells in the Adult Mouse Brain / Daniel G. Blackmore and Rodney L. Rietze -- Identifying Neural Progenitor Cells in the Adult Human Brain / Thomas I.H. Park [and seven others] -- Clinical Trials for the Treatment of Spinal Cord Injury: Not So Simple / Alan Mackay-Sim and François Féron.
  • 2008 Springer Protocols
    edited by Leslie P. Weiner.
    Definitions and criteria for stem cells / Leslie P. Weiner -- Neural stem cells and the future treatment of neurological diseases : raising the standard / Jaime Imitola and Samia J. Khoury -- Neural differentiation of human embryonic stem cells / Mirella Dottori and Martin F. Pera -- Generating neurons from stem cells / Andreas Androutsellis-Theotokis ... [et al.] -- Isolation of neural stem and precursor cells from rodent tissue / Yuanyuan Wu ... [et al.] -- Adult rodent spinal cord-derived neural stem cells : isolation and characterization / Lamya S. Shihabuddin -- Adult neural stem cells / Rossella Galli, Angela Gritti, and Angelo L. Vescovi -- Olfactory ensheathing cells : isolation and culture from the neonatal olfactory bulb / Susan C. Barnett and A. Jane Roskams -- Culturing olfactory ensheathing cells from the mouse olfactory epithelium / Miranda Richter, Kathryn Westendorf, and A. Jane Roskams -- Production and characterization of immortal human neural stem cell line with multipotent differentiation property / Seung U. Kim ... [et al.] -- Preparation of neural progenitors from bone marrow and umbilical cord blood / Shijie Song and Juan Sanchez-Ramos -- Production of neurospheres from CNS tissue / Gregory P. Marshall II ... [et al.] -- Nuclear transfer to study the nuclear reprogramming of human stem cells / Shigeo Saito ... [ et al.] -- Clonal analyses and cryopreservation of neural stem cell cultures / Angela Gritti, Rossella Galli, and Angelo L. Vescovi -- Telomere neurobiology / Mark P. Mattson, Peisu Zhang, and Aiwu Cheng -- Chromosomal mosaicism in neural stem cells / Suzanne E. Peterson ... [et al.] -- In vitro assays for neural stem cell differentiation : induction of dopaminergic phenotype / Marcel M. Daadi -- In vitro migration assays of neural stem cells / Pascale Durbec .. [et al.] -- Measuring apoptosis in neural stem cells / Brett T. Lund and Eve E. Kelland -- Markers of adult neural stem cells / Eric Wexler -- Differential gene expression in ES-derived neural stem cells by using RT-PCR / Nicole Slawny, Crystal Pacut, and Theresa E. Gratsch -- Microfluidic devices for high-throughput gene expression profiling of single hESC-derived neural stem cells / Yan Chen and Jiang F. Zhong -- Electroporation strategies for genetic manipulation and cell labeling / Terence J. Van Raay, Rhonda T. Lassiter, and Michael R. Stark -- Novel and immortalization-based protocols for the generation of neural CNS stem cell lines for gene therapy approaches / Luciano Conti and Elena Cattaneo -- Identification of newborn cells by BrdU labeling and immunocytochemistry in vivo / Sanjay S. Magavi and Jeffrey D. Macklis -- Immunocytochemical analysis of neuronal differentiation / Sanjay S. Magavi and Jeffrey D. Macklis -- Neuroanatomical tracing of neuronal projections with Fluoro-Gold / Lisa A. Catapano, Sanjay S. Magavi, and Jeffrey D. Macklis -- Labeling stem cells in vitro for identification of their differentiated phenotypes after grafting into the CNS / Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore -- Optimizing stem cell grafting into the CNS / Scott R. Whittemore ... [et al.] -- Routes of stem cell administration in the adult rodent / Alison E. Willing ... [et al.]
  • 2014 CRCnetBASE
    edited by Laurival Antonio De Luca Jr., José Vanderlei Menani, Alan Kim Johnson.
    "The importance of the regulation of salt is of clinical relevance given its relation to the "epidemic" of high blood pressure and that virtually all foods throughout the EU and USA will be mandated to reduce their salt content. This book covers virtually all aspects of the innate drives of thirst and salt, including transduction, sensory mechanism, afferent systems, organization, CNS integrative circuitry, and efferent pathways and effectors systems. It also covers the addition aspects of salt appetites and the orexigenic aspects, as well as other emerging topics in this field"--Provided by publisher.
  • 2013 Springer
    Mieczyslaw Pokorski, editor.
    Altered histone deacetylase activity and iNOS expression in cells isolated from induced sputum of COPD patients treated with tiotropium -- Involvement of NF-[kappa]B and muscle specific E3 ubiquitin ligase MuRF1 in cigarette smoke-induced catabolism in C2 myotubes -- Siglec-8 in induced sputum of COPD patients -- Essential amino acid leucine and proteasome inhibitor MG132 attenuate cigarette smoke induced catabolism in C2 myotubes -- Smoking denial in cardiovascular disease studies -- Differential effects of kainic acid lesions in medullary raphe on cough and sneeze in anesthetized rabbits -- Detection of Chlamydophila pneumoniae antigens in patients with chronic cough -- Antioxidant activity of herbal polysaccharides and cough reflex -- Cytoglobin and neuroglobin in the human brainstem and carotid body -- Flow cytometry in the diagnosis of influenza -- Accuracy of rapid influenza detection test in diagnosis of influenza A and B viruses in children less than 59 months old -- Virological monitoring of influenza activity and influenza-like illness in the epidemic season 2011-2012 in Poland -- Incidence of Chlamydophila pneumoniae infection in children during 2007-2010 -- Clinical features and outcomes of influenza A and B infections in children -- Respiratory complications in children hospitalized with Varicella -- Hyponatremia in children hospitalized due to pneumonia -- In vitro sensitivity of Acinetobacter baumannii and Pseudomonas aeruginosa to carbapenems among intensive care unit patients -- Prevalence of acute respiratory tract diseases among soldiers deployed for military operations in Iraq and Afghanistan -- In vitro susceptibility of Staphylococci and Enterococci to vancomycin and teicoplanin -- Sinusitis in people living in the Medieval Ages -- Immunization coverage against capsular bacteria in splenectomized patients -- Umbilical cord blood gas content, postnatal state of neonates, and lactation after caesarean and natural childbirth -- Respiratory function in pregnant women -- Lung function in patients with gastro-esophageal reflux disease and respiratory symptoms -- Efficacy of noninvasive mechanical ventilation in obese patients with chronic respiratory failure -- Near-infrared hemoencephalography for monitoring blood oxygenation in prefrontal cortical areas in diagnosis and therapy of developmental dyslexia -- Occlusal stabilization splint therapy in orofacial pain and tension-type headache -- Dynamics of upper airways during the Müller maneuver in healthy subjects: a cine MRI study -- EMG analysis of human inspiratory muscle resistance to fatigue during exercise -- Assessment of temporomandibular joint disease -- Spirometry Day: a means to enhance social knowledge on respiratory diseases -- Lung function in divers -- Influence of traffic-related air pollutants on lung function -- Fractional exhaled nitric oxide in clinical trials: an overview -- Real-time breath analysis in type 2 diabetes patients during cognitive effort -- Exhaled nitric oxide in specific inhalation challenge -- Integration of simulated multipotential signals: the role of integration window width and of the number of spikes -- Functional and topographic concordance of right atrial neural structures inducing sinus tachycardia -- Occurrence of disseminated intravascular coagulation in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced pneumonia in the rat -- Implementation of non-invasive methods in the diagnosis of diisocyanate-induced asthma -- Evaluation of a 4-steps-1-day whole body challenge protocol for the diagnosis of occupational asthma due to diisocyanates -- Occupational allergic respiratory diseases in garbage workers: relevance of molds and actinomycetes -- Th1, Th2, Th17, and regulatory cytokines in children with different clinical forms of allergy -- Soluble receptor for urokinase plasminogen activator in community-acquired pneumonia in children -- Hypersensitivity pneumonitis due to metalworking fluids: how to find the antigens -- Angiogenic axis angiopoietin-1 and angiopoietin-2/tie-2 in non-small cell lung cancer: a bronchoalveolar lavage and serum study -- Cancer incidence in pulmonary vasculitis -- Influence of delays in diagnosis and treatment on survival in small cell lung cancer -- Familial idiopathic pulmonary fibrosis -- High resolution computed tomography in 2-year follow-up of stage I sarcoidosis -- Altruistic aptitude: age-dependent influence of temperament and emotional intelligence -- Effect of socio-economic status on quality of life in people affected with respiratory allergy -- Exposure to paracetamol and antibiotics in early life and elevated risk of asthma in childhood -- Health behaviors and quality of life among patients with chronic respiratory disease -- Attitudes toward euthanasia among Polish physicians, nurses and people who have no professional experience with the terminally ill -- Death as a result of violent asphyxia in autopsy reports -- Respiratory and systemic infections in children with severe aplastic anemia on immunosupressive therapy.
    Also available: Print – 2013
  • 2011 Springer Protocols
    edited by Giovanni Manfredi, Hibiki Kawamata.
    Introduction to neurodegenerative diseases and related techniques -- In vivo and in vitro determination of cell death markers in neurons -- Degenerating processes identified by electron microscopic immunocytochemical methods -- Isolation and culture of neurons and astrocytes from the mouse brain cortex -- Isolation and culture of postnatal spinal motoneurons -- Converting human pluripotent stem cells to neural tissue and neurons to model neurodegeneration -- Neural differentiation of induced pluripotent stem cells -- Developing yeast models of human neurodegenerative disorders -- Modeling dopamine neuron degeneration in Caenorhabditis elegans -- Role of the proteasome in fly models of neurodegeneration -- Modeling neurodegenerative diseases in zebrafish embryos -- Practical considerations of genetic rodent models for neurodegenerative diseases -- Modeling focal cerebral ischemia in vivo -- Pharmacological models of Parkinson's disease in rodents.
  • 2009 Springer
    Michail S. Davidoff, Ralf Middendorff, Dieter Müller, Adolf F. Holstein.
  • 2013 Springer Protocols
    edited by Shohreh Amini, Martyn K. White.
    General overview of neuronal cell culture -- Considerations for the use of SH-SY5Y neuroblastoma cells in neurobiology -- Cultured cell line models of neuronal differentiation : NT2, PC12 -- Murine teratocarcinoma-derived neuronal cultures -- Isolation and propagation of primary human and rodent embryonic neural progenitor cells and cortical neurons -- Mouse enteric neuronal cell culture -- Preparation of neural stem cells and progenitors : neuronal production and grafting applications -- Derivation of neuronal cells from fetal Normal Human Astrocyte (NHA) -- Slice culture modeling of Central Nervous System (CNS) viral infection -- Neurospheres and glial cell cultures : immunocytochemistry for cell phenotyping -- Transfection of neuronal cultures -- Lentiviral transduction of neuronal cells -- Compartmentalized neuronal cultures -- Quantitative assessment of neurite outgrowth over growth promoting or inhibitory substrates.
  • 2015 Springer Protocols
    edited by Laura Lossi and Adalberto Merighi.
    Neuronal cell death : an overview of its different forms in central and peripheral neurons / Laura Lossi, Claudia Castagna, and Adalberto Merighi -- Cell volume regulation monitored with combined epifluorescence and digital holographic microscopy / Nicolas Pavillon and Pierre Marquet -- Flow cytometric analysis of DNA synthesis and apoptosis in central nervous system using fresh cell nuclei / Noelia López-Sánchez and José M. Frade -- Nuclear signs of pre-neurodegeneration / Fernando C. Baltanás ... [et al.] -- Multi-parametric O2 imaging in three-dimensional neural cell models with the phosphorescent probes / Ruslan I. Dmitriev and Dmitri B. Papkovsky -- Calcium imaging in neuron cell death / María Calvo, Carlos Villalobos, and Lucía Núñez -- Monitoring mitochondrial membranes permeability in live neurons and mitochondrial swelling through electron microscopy analysis / Macarena S. Arrázola and Nibaldo C. Inestrosa -- Real-time visualization of caspase-3 activation by fluorescence resonance energy transfer (FRET) / Silvia Alasia ... [et al.] -- Design and cloning of short hairpin RNAs (shRNAs) into a lentiviral silencing vector to study the function of selected proteins in neuronal apoptosis / Nadia Canu -- Genomic analysis using affymetrix standard microarray genechips (169 format) in degenerate murine retina / Sook Hyun Chung , Weiyong Shen , and Mark Gillies -- Genomic analysis of transcriptional changes underlying neuronal apoptosis / Sebastiano Cavallaro -- High-throughput cell death assays / Matthew E. Pamenter and Gabriel G. Haddad -- Staining of dead neurons by the golgi method in autopsy material / Stavros J. Baloyannis -- Image analysis algorithms for immunohistochemical assessment of cell death / Stan Krajewski ... [et al.] -- In vitro oxygen-glucose deprivation to study ischemic cell death / Carla I. Tasca, Tharine Dal-Cim, and Helena Cimarosti -- Laser microbeam targeting of single nerve axons in cell culture / Nicholas Hyun , Linda Z. Shi , and Michael W. Berns -- Real-time imaging of retinal cell apoptosis by confocal scanning laser ophthalmoscopy / Eduardo M. Normando ... [et al.] -- Targeted toxicants to dopaminergic neuronal cell death / Huajun Jin ... [et al.] -- Stem cells, neural progenitors, and engineered stem cells / Raj R. Rao and Shilpa Iyer -- Herpes simplex virus type 1 (HSV-1)-derived recombinant vectors for gene transfer and gene therapy / Peggy Marconi , Cornel Fraefel, and Alberto L. Epstein -- Herpes simplex virus type 1 (HSV-1)-derived amplicon vectors for gene transfer and gene therapy / Cornel Fraefel Peggy Marconi, and Alberto L. Epstein -- Bone marrow transplantation for research and regenerative therapies in the central nervous system / David Díaz, José Ramón Alonso, and Eduardo Weruaga -- Detection of activated caspase-8 in injured spinal axons by using fluorochrome-labeled inhibitors of caspases (FLICA) / Antón Barreiro-Iglesias and Michael I. Shifman -- Generation of zebrafish models by CRISPR/Cas9 genome editing / Alexander Hruscha and Bettina Schmid -- In vivo assessment of neuronal cell death in Drosophila / Pierre Dourlen -- Drosophila model for studying phagocytosis following neuronal cell death / Boris Shklyar, Flonia Levy-Adam, and Estee Kurant.
  • 2016 ScienceDirect
    edited by K. Kevin Pfister.
  • 2013 CRCnetBASE
    Raymond C. Valentine, David L. Valentine.
    Nature of neurons and their specialized membranes -- DHA contributes both benefits and risks to mammals -- Use it or lose it concept of brain health is linked to DHA -- Evolution and DHA : redefining the DHA principle -- DHA is king omega for maximizing mental speed -- DHA improves energy efficiency in neurons -- Wild conformational dynamics of DHA might save energy by sealing stretch-induced defects as fast as they occur in membranes -- Lipid whisker model and water-wire theory of energy uncoupling in neurons -- Neurons boost their energy state by using powerful antioxidant systems to protect their membranes against damage -- Oxygen-dependent damage (lipid peroxidation) of DHA membranes of neurons is inevitable and requires novel mechanisms for long-term protection -- Revised mitochondrial theory of aging and brain span -- Development of dual energy pacemaker theory of aging : role of the membrane -- Membranes and cancer -- Parkinson's disease -- Prion diseases -- Brain trauma-induced dementia -- Alzheimer's disease.
  • 2015 Wiley
    edited by William E. Armstrong, Jeffrey G. Tasker.
    Electrophysiology of magnocellular neurons in vivo -- Oxytocin neurons during suckling : lessons from organotypic cultures -- Peptidergic control of oxytocin and vasopressin neurons and its role in reproductive and hypertension-associated plasticity -- The osmotic control of vasopressin releasing neurons -- Function and localization of epithelial sodium channels in vasopressin and oxytocin neurons -- Visible markers of vasopressin and oxytocin activity and their use in identifying the neuronal activity of specific neuroendocrine cell types -- Neurophysiology of neurohypophysial terminals -- Neuronal-glia remodeling of the magnocellular system -- Dendritic release of the neuropeptides vasopressin and oxytocin -- Endocannabinoid modulation of synaptic inputs to magnocellular neurons -- Role of central vasopressin in the generation of multimodal homeostatic responses -- Elucidating the structure and function of gonadotropin-releasing hormone (GnRH) neuron dendrites -- Roles of estrogen and kisspeptin in controlling gonadotropin releasing hormone (GnRH) neuronal excitability -- Multiple-unit activity recording of the gonadotropin-releasing hormone pulse generator.
  • 2007 Springer Protocols
    edited by Tiziana Borsello.
    Neuronal death and neuroprotection : a review / Mariaelena Repici, Jean Mariani, and Tiziana Borsello -- The use of propidium iodide to assess excitotoxic neuronal death in primary mixed cortical cultures / Anthony C. Lau, Hong Cui, and Michael Tymianski -- A modified silver technique (de Olmos Stain) for assessment of neuronal and axonal degeneration / Tatyana I. Tenkova and Mark P. Goldberg -- Model of acute injury to study neuroprotection / Michal Schwartz and Jonathan Kipnis -- Organotypic entorhino-hippocampal slice cultures : a tool to study the molecular and cellular regulation of axonal regeneration and collateral sprouting in vitro / Domenico Del Turco and Thomas Deller -- Role of Nrf2-dependent ARE-driven antioxidant pathway in neuroprotection / Jiang Li ... [et al.] -- Biochemical methods to assess the coupling of brain energy metabolism in control and disease states / Jennifer Zechel, W. David Lust, and Michele Puchowicz -- A method for isolating prosurvival targets of NF-[kappa]B/rel transcription factors / Christian Kuntzen ... [et al.] -- Functional cloning of genes regulating apoptosis in neuronal cells / Roberta Visconti and Luciano D'Adamio -- Characterization of mRNA expression in single neurons / David M. Lin ... [et al.] -- Brain on a chip : a method to detect novel neuroprotective candidate targets / Yang Tang and Myriam Bernaudin -- Intracerebral infusion of neurotrophic factors / Theo Hagg -- Synthesis of cell-penetrating peptides and their application in neurobiology / Gunnar P.H. Dietz and Mathias Bähr -- The stem cells as a potential treatment for neurodegeneration / Ferrari Daniela, Angelo Luigi Vescovi, and Daniele Bottai -- In vivo tomographic imaging studies of neurodegeneration and neuroprotection : a review / Rikki N. Waterhouse and Jun Zhao.
  • 2014 Springer Protocols
    edited by Mark T. Quinn, Frank R. DeLeo.
    The role of neutrophils in the immune system : an overview / Harry L. Malech, Frank R. DeLeo, and Mark T. Quinn -- Isolation of human neutrophils from venous blood / William M. Nausef -- Neutrophil isolation from nonhuman species / Daniel W. Siemsen ... [and 7 others] -- Collection of in vivo transmigrated neutrophils from human skin / Karin Christenson ... [and 6 others] -- Subcellular fractionation of human neutrophils and analysis of subcellular markers / Stine Novrup Clemmensen, Lene Udby, and Niels Borregaard -- Rho family and Rap GTPase activation assays / Richard T. Jennings and Ulla G. Knaus -- Measurement of phospholipid metabolism in intact neutrophils / Susan Sergeant and Linda C. McPhail -- Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils / Maurice B. Hallett, Maha Al-Jumaa, and Sharon Dewitt -- Analysis of electrophysiological properties and responses of neutrophils / Deri Morgan and Thomas E. DeCoursey -- Assessment of neutrophil apoptosis / David A. Dorward ... [and 3 others] -- Microinjection methods for neutrophils / Iraj Laffafian ... [and 3 others] -- Generation of functionally mature neutrophils from induced pluripotent stem cells / Colin L. Sweeney ... [and 6 others] -- Neutrophil migration through extracellular matrix / Richard T. Jennings and Ulla G. Knaus -- Spinning disk confocal imaging of neutrophil migration in zebrafish / Pui-Ying Lam ... [and 4 others] -- Detection of bidirectional signaling during integrin activation and neutrophil adhesion / Stuart M. Altman, Neha Dixit, and Scott I. Simon -- Immunofluorescence and confocal microscopy of neutrophils / Lee-Ann H. Allen -- Expressin of genetically encoded fluorescent probes to monitor phospholipid dynamics in live neutrophils / Benjamin E. Steinberg, Marco A.O. Magalhaes, and Sergio Grinstein -- Quantitative assessment of neutrophil phagocytosis using flow cytometry / Pontus Nordenfelt -- Analysis of neutrophil bactericidal activity / Heather A. Parker ... [and 4 others] -- Induction and quantification of neutrophil extracellular traps / Alejandro Sanchez Gonzalez ... [and 3 others] -- Measurement of respiratory burst products, released or retained, during activation of professional phagocytes / Johan Bylund ... [and 4 others] -- Cell-free NADPH oxidase activation assays : "In vitro veritas" / Edgar Pick -- Assessment of priming of the human neutrophil respiratory burst / Margarita Hurtado-Nedelec ... [and 4 others] -- Affinity pruification and reconstitution of human phagocyte flavocytochrome b for detection of conformational dynamics in the membrane / Marcia Riesselman and Algirdas J. Jesaitis -- Evaluation of p47phox phosphorylation in human neutrophils using phospho-specicfic antibodies / Sahra Amel Belambri, Pham My-Chan Dang, and Jamel El-Benna -- Genome-scale transcript analyses with human neutrophils / Scott D. Kobayashi, Daniel E. Sturdevant, and Frank R. DeLeo -- Fast and accurate quantitative analysis of cytokine gene expression in human neutrophils / Nicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni -- High-purity neutrophil isolation from human peripheral blood and saliva for transcriptome analysis / Flavia S. Lakschevitz and Michael Glogauer -- Detection of intact transciption factors in human neutrophils / Patrick P. McDonald and Richard D. Ye -- Disorders of neutrophil function : an overview / Mary C. Dinauer -- Diagnostic assays for chronic granulomatous disease and other neutrophil disorders / Houda Zghal Elloumi and Steven M. Holland -- Diagnostic assays for myeloperoxidase and myeloperoxidase deficiency / William M. Nauseef.
  • 2007 Springer Protocols
    edited by Mark T. Quinn, Frank R. DeLeo, Gary M. Bokoch.
    The role of neutrophils in the immune system: an overview / H.L. Malech -- Subcellular fractionation of human neutrophils and analysis of subcellular markers / L. Udby and N. Borregaard -- Rac and Rap GTPase activation assays / U.G. Knaus, A. Bamberg and G.M. Bokoch -- Measurement of phospholipid metabolism in intact neutrophils / S. Sergeant and L.C. McPhail -- Analysis of protein phosphorylation in human neutrophils / J. El-Benna and P.M.-C. Dang -- Mitogen-activated protein kinase assays / M. Torres -- Transduction of proteins into intact neutrophils / T. Zhao and G.M. Bokoch -- Optical methods for the measurement and manipulation of cytosolic free calcium in neutrophils / E.J. Hillson, S. Dewitt and M.B. Hallett -- Analysis of electrophysiological properties and responses of neutrophils / D. Morgan and T.E. DeCoursey -- Analysis of neutrophil apoptosis / E.L. Taylor ... [et al.] -- Optical and fluorescence detection of neutrophil integrin activation / U.Y. Schaff ... [et al.] -- Analysis of neutrophil polarization and chemotaxis / M.A. Lokuta, P.A. Nuzzi and A. Huttenlocher -- Quantifying and localizing actin-free barbed ends in neutrophils / M. Glogauer -- Measurement of neutrophil adhesion under conditions mimicking blood flow / M.A. Jutila ... [et al.] -- Model systems to investigate neutrophil adhesion and chemotaxis / N.A. Louis, E. Campbell and S.P. Colgan -- Immunofluorescence and confocal microscopy of neutrophils / L.-A.H. Allen -- Assessment of phagosome formation and maturation by fluorescence microscopy / B.E. Steinberg and S. Grinstein -- Analysis of neutrophil membrane traffic during phagocytosis / P. Loennbro, P. Nordenfelt and H. Tapper -- Analysis of neutrophil bactericidal activity / J.N. Green, C.C. Winterbourn and M.B. Hampton -- A skin chamber technique as a human model for studies of aseptic inflammatory reactions / P. Follin and C. Dahlgren -- Measurement of respiratory burst products generated by professional phagocytes / C. Dahlgren, A. Karlsson and J. Bylund -- The K-562 cell model for analysis of neutrophil NADPH oxidase function / T.L. Leto ... [et al.] -- Cell-free assays: the reductionist approach to the study of NADPH oxidase assembly, or "all you wanted to know about cell-free assays but did not dare to ask" / S. Molshanski-Mor ... [et al.] -- Immunoaffinity purification of human phagocyte flavocytochrome b and analysis of conformational dynamics / R.M. Taylor and A.J. Jesaitis -- Genome-scale transcript analyses in human neutrophils / S.D. Kobayashi, D.E. Sturdevant and F.R. DeLeo -- Fast and accurate quantitative analysis of cytokine gene expression in human neutrophils by reverse transcription real-time PCR / N. Tamassia, M.A. Cassatella and F. Bazzoni -- Detection of intact transcription factors in human neutrophils / P.P. McDonald and R.D. Ye -- Disorders of neutrophil function: an overview / M.C. Dinauer -- Diagnostic assays for chronic granulomatous disease and other neutrophil disorders / H.Z. Elloumi and S.M. Holland -- Diagnostic assays for myeloperoxidase deficiency / W.M. Nauseef.
  • 2007 Springer
    M. Kusano, S. Shioda (eds.).
  • 2012 Springer
    Avi Ma'ayan, Ben D. MacArthur, editors.
    Gene Expression Profiling and Regulatory Networks in Single Cells / Fumio Arai, Kentaro Hosokawa, Yoshiko Matsumoto, Hirofumi Toyama and Toshio Suda -- Cell Fate Regulatory Networks / Sonya J. Ridden and Ben D. MacArthur -- Noise in Regulatory Networks / Srinandan Dasmahapatra -- Generalised Modelling in Systems Biology / Thilo Gross -- Relative Quantification Mass Spectrometry Using iTRAQ Isobaric Tags / Richard D. Unwin, Emma Carrick and Anthony D. Whetton -- Deciphering Protein Complexes and Protein Interaction Networks for Stem Cell Pluripotency / Jianlong Wang -- Omics Era in Stem Cell Research: Data Integration of Multi-regulatory Layers / Ana Sevilla, Benjamin Trinite and Ihor R. Lemischka -- High Throughput Affinity Purification and Mass Spectrometry to Determine Protein Complex Interactions / Anna Malovannaya, Rainer B. Lanz, Bert W. O'Malley and Jun Qin -- Network Pharmacology to Aid the Drug Discovery Process / Ruth Dannenfelser, Huilei Xu, Catherine Raimond and Avi Ma'ayan -- What Can Networks Do for You? / Bernhard M. Schuldt, Franz-Josef Müller and Andreas A. Schuppert.
  • 2005 Karger
    volume editors, Philippe Poindron, Pascale Piguet, Eckart Förster.
    Hippocampal slice cultures / Förster, E., Bartos, M., Zhao, S. -- Protocol for the primary culture of cortical neurons / Gonthier, B. ... [et al.] -- A new technique to control the architecture of neuronal networks in vitro / Wyart, C. ... [et al.] -- Organotypic cultures of the rat retina / Hofmann, H.-D. ... [et al.] -- Cerebellar slice cultures / Kapfhammer, J.P. -- Culture of dissociated hippocampal neurons / Rigato, F. -- Improved methods for culturing rat and mouse motor neurons. Their applications / Guettier-Sigrist, S. ... [et al.] -- Characterization of long-term cultured cell lines of murine microglia called MAMI. The case for MAMI-96 cell line / Laporte, V. ... [et al.].
  • 2002 ScienceDirect
    edited by Enrique Cadenas, Lester Packer.
    Also available: Print – 2002
  • 2014 Springer
    Muller Fabbri, editor.
    Introduction / Muller Gabbri -- Biogenesis and physiology of microRNAs / Carlos A. Melo and Sonia A. Melo -- Detecting noncoding RNA expression : from arrays to next-generation sequencing / Catalina Perdomo, Joshua Campbell, and Frank Schembri -- MicroRNAs in solid tumors / Jin Wang and Subrata Sen -- MicroRNAs in hematologic malignancies / Johannes Bloehdorn ... [et al.] -- miRNAs as cancer biomarkers / Edward R. Sauter -- Unraveling the complex network of interactions between noncoding RNAs and epigenetics in cancer / Veronica Davalos and Manel Esteller -- MicroRNA SNPs in cancer / Yujing LI and Peng Jin -- Bioinformatics approaches to the study of MircoRNAs / Ravi Gupta and Ramana V. Davuluri -- Beyond miRNAs : role of other noncoding RNAs in cancer / Roxana S. Redis and George A. Calin -- Translational implication for noncoding RNA in cancer / Duaa Dakhallah, Melissa Piper, and S. Patrick Nana-Sinkam.
  • 2014 Springer Protocols
    edited by Hugo J. Bellen, Shinya Yamamoto.
    Introduction to notch signaling / Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen -- Genetic screens to identify new notch pathway mutants in Drosophila / Nikolaos Giagtzoglou -- Structure-function analysis of Drosophila notch using genomic tescue transgenes / Jessica Leonardi and Hamed Jafar-Nejad -- Overview of genetic tools and techniques to study notch signaling in mice / Thomas Gridley and Andrew K. Groves -- Immunohistochemical tools and techniques to visualize notch in Drosophila melanogaster / Emiliana Tognon and Thomas Vaccari -- Antibody uptake assay and in vivo imaging to study intracellular trafficking of notch and delta in Drosophila / Lydie Couturier and François Schweisguth -- Tracking trafficking of notch and its ligands in mammalian cells / Patricia Chastagner and Christel Brou -- Visualizing notch signaling in vivo in Drosophila tissues / Benjamin E. Housden, Jinghua Li, and Sarah J. Bray -- Monitoring notch activity in the mouse / Swananda Marathe and Lavinia Alberi -- Notch signaling assays in Drosophila cultured cell lines / Jinghua Li, Benjamin E. Housden, and Sarah J. Bray -- Monitoring notch activation in cultured mammalian cells : transcriptional reporter assays / Ma. Xenia G. Ilagan and Raphael Kopan -- Monitoring notch activation in cultured mammalian cells : luciferase complementation imaging assays / Ma. Xenia G. Ilagan and Raphael Kopan -- Visualization of notch signaling oscillation in cells and tissues / Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama -- Proteomic analysis of the notch interactome / K. G. Guruharsha ... [et al.] -- Bacterial expression and in vitro refolding of limited fragments of the notch receptor and its ligands / Pat Whiteman, Christina Redfield, and Penny A. Handford -- Analyzing the posttranslational modification status of notch using mass spectrometry / Shinako Kakuda and Robert S. Haltiwanger -- Assay to probe proteolytic processing of notch by [gamma]-secretase / Lutgarde Serneels, Ina Tesseur, and Bart De Strooper -- Analyzing the nuclear complexes of notch signaling by electrophoretic mobility shift assay / Kelly L. Arnett and Stephen C. Blacklow -- Identifying direct notch transcriptional targets using the GSI-washout assay / Will Bailis, Yumi Yashiro-Ohtani, and Warren S. Pear -- Probing the epigenetic status at notch target genes / Robert Liefke and Tilman Borggrefe -- Notch-ligand binding assays in Drosophila cells / Aiguo Xu and Kenneth D. Irvine -- Modeling notch signaling : a practical tutorial / Pau Formosa-Jordan and David Sprinzak -- Small molecules that inhibit notch signaling / Gerdien E. De Kloe and Bart De Strooper -- Application and evaluation of anti-notch antibodies to modulate notch signaling / Wendy R. Gordon and Jon C. Aster -- Application of anti-ligand antibodies to inhibit notch signaling / Jun-ichiro Koga and Masanori Aikawa.
  • 2015 Springer
    Christopher Ahern, Stephan Pless, editors.
    Introduction -- Engineered ionized side chains -- Cysteine modification: probing channel structure, function, and conformational change -- Functional site-directed fluorometry -- Bioreactive tethers -- Flipping the photoswitch: ion channels under light control -- Incorporation of non-canonical amino acids -- Index.
    Also available: Print – 2015
  • 2012 Springer
    Stefan Fruehauf, W. Jens Zeller, Gary Calandra, editors.
    Part 1. Basic Science and Experimental Studies on Stem Cell Mobilization -- Preclinical Experience with AMD3100 for Mobilization of Hematopoietic Stem and Progenitor Cells -- Molecular Pharmacology of CXCR4 Inhibition -- Combination Strategies for Stem Cell Mobilization Targeting CXCR4 and S1P Receptors -- Should We Stay or Should We Go Now? -- Cell Adhesion and Mobilization Pathways of Hematopoietic Cells -- Role of the CXCR4/CXCL12 Axis in Hematopoietic Stem Cell Trafficking -- Part 2. Clinical Studies on Stem Cell Mobilization -- Front matter -- Early Studies of AMD3100/Plerixafor in Healthy Volunteers -- The Current Role of Plerixafor in Stem Cell Mobilization for Hematopoietic Stem Cell Transplantation -- Experience with Apheresis Procedures After Plerixafor Mobilisation -- Influence of Different Stem Cell Mobilization Strategies on Graft Composition and Outcome of Autologous or Allogeneic Transplantation -- Relevance and Clinical Implications of Tumor Cell Mobilization in Autologous Transplantation of Multiple Myeloma and Non-Hodgkin's Lymphoma -- Plerixafor: Data from the Compassionate Use Program -- Stem Cell Mobilization with Chemomobilization Plus Plerixafor in Hard-to-Mobilize Patients: The Experience at Kuopio University Hospital, Finland -- Blood Stem Cell Mobilization in Solid Tumors: Experience with Plerixafor and Alternative Ways of Hematopoietic Stem Cell Mobilization -- Mobilization with Chemotherapy + G-CSF + Plerixafor in Europe: The Italian Experience -- Experience with Plerixafor in Poor Mobilizers -- CXCR4 Antagonists -- Part 3. CXCR4 Antagonists for Treatment of Leukemias and Solid Tumors -- Front matter -- Application of CXCR4 Inhibitors in Leukemia -- CXCR4 Antagonists for the Treatment of CML -- Targeting CXCR4 in Chronic Lymphocytic Leukemia: Preclinical Rationale and Early Clinical Experience -- CXCR4-Blockade for Treatment of Breast Cancer -- Molecular Imaging of CXCR4 Receptor Expression in Tumors -- Part 4. Novel applications -- Potential Use of CXCR4 Antagonists to Mobilize Endothelial and Mesenchymal Stem Cells -- Strategies to Enhance Hematopoietic Stem Cell Engraftment Following Transplantation -- Mobilization for Gene Therapy.
  • 2012
    Michael Robert Mancuso.
    Judah Folkman first hypothesized in 1971 that tumor growth depends on angiogenesis, the growth of new blood vessels from pre-existing ones. Eighteen years later, in 1989, Harold Dvorak and Napoleana Ferrara independently isolated Vascular Endothelial Growth Factor (VEGF), which Dr. Ferrara went on to prove to be one of the most important endothelial cell mitogens ever discovered. In 2004, the FDA approved the use of Bevacizumab, a monoclonal antibody against VEGF, for the treatment of colon cancer after the drug showed superiority when combined with a standard chemotherapy regimen. While the use of VEGF inhibitors in the clinic has shown benefit in treating patients, there is room for clinical improvement. This thesis was initially established on the concept that the identification of other novel regulators of angiogenesis could provide additional targets for anti-angiogenic therapy to be used either alone or in combination with VEGF inhibition to achieve results superior to VEGF blockade alone. Against this background, mouse models of conditional Egfl7, Egfl8, miR-126, and GPR124 genetic deletion were designed and generated to determine the function of these novel molecules in the context of regulating angiogenesis. We discovered that the previously described Egfl7 mouse knockout phenotypes of 50% embryonic lethality with edema and delayed cranial blood vessel formation and post-natal vascularization of the retina were actually due to disruption of the microRNA miR-126 residing in the seventh intron of Egfl7. We further showed that miR-126 regulates angiogenesis through suppressing expression of p85[beta] and Spred1 which inhibit the downstream signaling cascade of VEGF signaling. Surprisingly, the Egfl7 mice were phenotypically normal even with simultaneous deletion of the paralog Egfl8. We noted that the previous study inaccurately described the miR-126 angiogenic phenotypes as those of Egfl7 after using insertional deletion ("knockin") strategies to develop mouse models of Egfl7 gene deletion which inadvertently disrupted expression of miR-126. We highlight that this concept complicates the design of mouse genetic deletion studies and recommend that strategies which provide minimal deletion of a region of the locus are superior to "knockin" strategies. GPR124 is a known requirement of developmental angiogenesis specifically within the Central Nervous System (CNS). As GPR124 is expressed by endothelial cells and pericytes in the CNS, it was unknown at the start of my graduate career if endothelial cell or pericyte expression was required for blood vessel migration into the CNS. Using an endothelial cell specific driver, we were able to delete GPR124 specifically within endothelial cells and prove that endothelial cell expression of GPR124 drove vascularization of the CNS during development, thus proving that GPR124 functions endothelial cell autonomously. Further, as embryos lacking GPR124 die with 100% penetrance before birth, I crossed my conditional allele with an inducible driver to delete GPR124 in adulthood in order to bypass the window of embryonic lethality. I found that GPR124 was not required for homeostasis of the quiescent cerebrovasculature. Using orthotopic glioma and transient middle cerebral artery occlusion studies, we found that mice lacking GPR124 were susceptible to intracranial hemorrhage after insult. These results showed that GPR124 plays an important role in maintaining cerebrovacular integrity in the context of disease within the CNS. Overall, this thesis has identified, clarified or further described the functional role of several novel regulators of angiogenesis. Future studies beyond the scope of this thesis will use the mouse models of conditional gene deletion built for this dissertation to further investigate the mechanisms through which these genes regulate angiogenesis. Hopefully, further understanding of how each of these novel genes regulates endothelial cell physiology will provide valuable contributions towards improving out ability to treat cancer through manipulation of its blood supply.
  • 2005 Springer
    Zofia Zukowska, Giora Z. Feuerstein, editors.
    Also available: Print – 2005
  • 2015 Springer Protocols
    edited by Shinichi Nakagawa, Tetsuro Hirose.
    Visualization of lncRNA by single-molecule fluorescence in situ hybridization / Margaret Dunagin , Moran N. Cabili , John Rinn , and Arjun Raj -- Super-resolution imaging of nuclear bodies by STED microscopy / Yasushi Okada and Shinichi Nakagawa -- High-resolution 3D DNA FISH using plasmid probes and computational correction of optical aberrations to study chromatin structure at the sub-megabase scale / Luca Giorgetti, Tristan Piolot, and Edith Heard -- Time-lapse imaging of nuclear bodies / Saskia Hutten, Samuel Swift, and Angus I. Lamond -- Visualization of nucleic acids with synthetic exciton-controlled fluorescent oligonucleotide probes / Dan Ohtan Wang and Akimitsu Okamoto -- Live CLEM imaging to analyze nuclear structures at high resolution / Tokuko Haraguchi, Hiroko Osakada, and Takako Koujin -- Ultrastructural analysis of nuclear bodies using electron microscopy / Sylvie Souquere and Gérard Pierron -- Analyses of nuclear proteins and nucleic acid structures using atomic force microscopy / Jamie L. Gilmore [and eight others] -- Genome-wide co-localization screening of nuclear body components using a fluorescently tagged FLJ cDNA clone library / Tetsuro Hirose and Naoki Goshima -- Purification of specific chromatin regions using oligonucleotides : capture hybridization analysis of RNA targets (CHART) / Christopher P. Davis and Jason A. West -- RNA antisense purification (RAP) for mapping RNA interactions with chromatin / Jesse Engreitz, Eric S. Lander, and Mitchell Guttman -- In situ dissection of RNA functional subunits by domain-specific chromatin isolation by RNA purification (dChIRP) / Jeffrey J. Quinn and Howard Y. Chang -- Extracting, enriching, and identifying nuclear body sub-complexes using label-based quantitative mass spectrometry / Archa Fox [and three othrs] -- Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells : native ribonucleoprotein immunoprecipitation (RIP) assays / Joseph A. Cozzitorto [and eight others] -- Cross-linking and immunoprecipitation of nuclear RNA-binding proteins / Quan Li , Yuri Uemura , and Yukio Kawahara -- Purification of noncoding RNA and bound proteins using FLAG peptide-conjugated antisense-oligonucleotides / Shungo Adachi and Tohru Natsume -- MMCT-mediated chromosome engineering technique applicable to functional analysis of lncRNA and nuclear dynamics / Makiko Meguro-Horike and Shin-ichi Horike -- Reconstitution of nucleocytoplasmic transport using digitonin-permeabilized cells / Shingo Kose, Tomoko Funakoshi, and Naoko Imamoto -- Genome-wide analysis of long noncoding RNA turnover / Hidenori Tani [and eight others] -- Knockdown of nuclear-retained long noncoding RNAs using modified DNA antisense oligonucleotides / Xinying Zong [and six others] -- siRNA screening of nuclear proteins / Yuko Hasegawa and Shinichi Nakagawa.
  • 2007 Springer
    K. Nagata, K. Takeyasu (eds.).
  • 2010 ScienceDirect
    edited G.V. Shivashankar.
    Section A. Cell Nucleus: Organization & MechanoBiology -- Chapter 1. Fluorescence Fluctuation Microscopy to Reveal 3D Architecture and Function in the Cell Nucleus / Thorsten Lenser, Klaus Weisshart, Tobias Ulbricht, Karolin Klement, Peter Hemmerich -- Chapter 2. Studying Histone Modifications and Their Genomic Functions by Employing Chromatin Immunoprecipitation and Immunoblotting / Ranveer S. Jayani, Praveena L. Ramanujam, Sanjeev Galande -- Chapter 3. Dynamic Organization of Chromatin Assembly and Transcription Factories in Living Cells / Bidisha Sinha, Dipanjan Bhattacharya, Deepak Kumar Sinha, Shefali Talwar, Shovamayee Maharana, Soumya Gupta, G.V. Shivashankar -- Chapter 4. Manipulation and Isolation of Single Cells and Nuclei / Swee Jin Tan, Qingsen Li, Chwee Teck Lim -- Chapter 5. Beyond Lamins: Other Structural Components of the Nucleoskeleton / Zhixia Zhong, Katherine L. Wilson, Kris Noel Dahl -- Chapter 6. Altered Mechanical Properties of the Nucleus in Disease / Maria Lucia Lombardi, Jan Lammerding -- Chapter 7. Theoretical Concepts and Models of Cellular Mechanosensing / Rumi De, Assaf Zemel, Samuel A. Safran -- Section B. Impact of Nuclear Mechanics on Function -- Chapter 8. Mechanical Induction of Gene Expression in Connective Tissue Cells / Matthew W.C. Chan, Boris Hinz, Christopher A. McCulloch -- Chapter 9. Physical Plasticity of the Nucleus and its Manipulation / Irena Ivanovska, Joe Swift, Takamasa Harada, J. David Pajerowski, Dennis E. Discher -- Chapter 10. Prestressed Nuclear Organization in Living Cells / Aprotim Mazumder, T. Roopa, Abhishek Kumar, K. Venkatesan Iyer, Nisha M. Ramdas, G.V. Shivashankar -- Chapter 11. Nanotopography/Mechanical Induction of Stem-Cell Differentiation / Benjamin Kim Kiat Teo, Soneela Ankam, Lesley Y. Chan, Evelyn K.F. Yim -- Chapter 12. Mechanical Induction in Embryonic Development and Tumor Growth: Integrative Cues Through Molecular to Multicellular Interplay and Evolutionary Perspectives / Maria-Elena Fernandez-Sanchez, Fanny Serman, Padra Ahmadi, Emmanuel Farge -- Chapter 13. Informatics-Based Analysis of Mechanosignaling in the Laminopathies / Frank P.L. Lai, Radfidah A. Mutalif, Siew Cheng Phua, Colin L. Stewart -- Chapter 14. Autosomal Dominant Leukodystrophy Caused by Lamin B1 Duplications: A Clinical and Molecular Case Study of Altered Nuclear Function and Disease / Quasar Saleem Padiath, Ying-Hui Fu.
  • 2014 ScienceDirect
    edited by Valérie Doyle.
    Volume 122 of Methods in Cell Biology describes modern tools and techniques used to study nuclear pore complexes and nucleocytoplasmic transport in diverse eukaryotic model systems (including mammalian cells, Xenopus, C. elegans, yeast). The volume enables investigators to analyze nuclear pore complex structure, assembly, and dynamics; to evaluate protein and RNA trafficking through the nuclear envelope; and to design in vivo or in vitro assays appropriate to their research needs.
  • 2009 Springer Protocols
    edited by Iain J. McEwan.
    Nuclear receptors : one big family / Iain J. McEwan -- Methods for measuring ligand dissociation and nuclear receptor turnover in whole cells / Elizabeth M. Wilson -- Flow cytometry as a tool for measurement of steroid hormone receptor protein expression in leukocytes / Cherie L. Butts and Esther M. Sternberg -- X-ray crystallography of agonis/antagonist-bound receptors / Ashley C.W. Pike -- FRAP and FRET methods to study nuclear receptors in living cells / Martin E. van Royen ... [et al.] -- Receptor-DNA interactions : EMSA and footprinting / Jason T. Read ... [et al.] -- Chromatin immunoprecipitation (ChIP) methodology and readouts / Charles E. Massie and Ian G. Mills -- Yeast-based reporter assays for the functional characterization of cochaperone interactions with steroid hormone receptors / Heather A. Balsiger and Marc B. Cox -- High throughput analysis of nuclear receptor-cofactor interactions / Michael L. Goodson, Behnom Farboud, and Martin L. Privalsky -- Binding affinity and kinetic analysis of nuclear receptor/co-regulator interactions using surface plasmon resonance / Derek N. Lavery -- Using RNA interference to study protein function / Carol D. Curtis and Ann M. Nardulli -- Using intrinsic fluorescence emission spectroscopy to study steroid receptor and coactivator protein conformation dynamics / Kate Watt and Iain J. McEwan -- Development of phosphorylation site-specific antibodies to nuclear receptors / Inés Pineda Torra ... [et al.] -- Tissue-selective knockouts of steroid receptors : a novel paradigm in the study of steroid action / Karel De Gendt and Guido Verhoeven -- Methods for identifying and studying genetic alterations in hormone-dependent cancers / Outi R. Saramäki, Kati K. Waltering, and Tapio Visakorpi.
  • 2010 Springer
    edited by Chris M. Bunce, Moray J. Campbell.
    Nuclear receptors an introductory overview / C.M. Bunce and M.J. Campbell -- What does evolution teach us about nuclear receptors? / G. Markov, F. Bonneton and V. Laudet -- Functions of nuclear receptors in insect development / D. Martín -- The glucocorticoid receptor / R.H. Oakley and J.A. Cidlowski -- Estrogen receptors: Their actions and functional roles in health and disease / S. Nilsson and J.-Å. Gustafsson -- Androgen receptor / J.T. Dalton and W. Gao -- Thyroid hormone receptors / B. Vennström, H. Liu and D. Forrest -- The vitamin D receptor (NR1I1) / O. Maguire and M.J. Campbell -- Retinoic acid receptors / A. Cras, F. Guidez and C. Chomienne -- PPARs: Important regulators in metabolism and inflammation / L.M. Sanderson and S. Kersten -- Xenobiotic receptors CAR and PXR / C. Klaassen and H. Lu -- Fxr / Y. Wang ... [et al.] -- Physiological functions of TR2 and TR4 orphan nuclear receptor / S. Liu ... [et al.] -- Nuclear receptors and ATP dependent chromatin remodeling: A complex story / C.J. Burd and T.K. Archer -- Non-genomic action of sex steroid hormones / A. Migliaccio, G. Castoria and F. Auricchio -- Ligand regulation and nuclear receptor action / M. Hewison -- New insights to nuclear receptor gene regulation from analysis of their response elements in target genes / C. Carlberg -- Systems biology: Towards realistic and useful models of molecular networks / F.J. Bruggeman ... [et al.].
  • 2015 Springer Protocols
    edited by Nathalie Beaujean, Hélène Jammes, Alice Jouneau.
    Nuclear Transfer in the Mouse -- Nuclear Transfer in the Rabbit -- Nuclear Transfer in Ruminants -- Nuclear Transfer and Transgenesis in the Pig -- Embryonic Stem Cell-Somatic Cell Fusion and Post-Fusion Enucleation -- Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte -- Assessing the Quality of Donor Cells: Karyotyping Methods -- Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer -- Fluorescent Immunodetection of Epigenetic Modifications on Pre-Implantation Mouse Embryos -- Visualization of Epigenetic Modifications in Preimplantation Embryos -- Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer -- Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming -- Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining -- Gene Expression Analysis in Early Embryos through Reverse Transcription Quantitative PCR (RT-qPCR) -- Studying Bovine Early Embryo Transcriptome by Microarray -- Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level -- Micro Chromatin Immunoprecipitation ({uF06D}ChIP) from Early Mammalian Embryos -- Assessing Reprogramming by Chimera Formation and Tetraploid Complementation -- Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology -- Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP).
  • 2011 Springer
    Justin Ainscough, Shinya Yamanaka, Takashi Tada, editors.
    Annotation Research into the field of stem cell biology has developed exponentially over recent years, and is beginning to offer significant promise for unravelling the molecular basis of a multitude of disease states. Importantly, in addition to offering the opportunity to delve deeply into the mechanisms that drive disease aetiology the research is realistically opening the doors for development of targeted and personalized therapeutic applications that many considered, until recently, to be nothing more that a far fetched dream. This volume provides a timely glimpse into the methods that have been developed to instigate, and the mechanisms that have been identified to drive, the process of nuclear reprogramming, chronicling how the field has developed over the last 50-60 years. Since the early 1950s a small number of notable experiments have provided significant impetus to the field, primarily the demonstration of reprogramming ability, first by the complex cytoplasmic milieu that constitutes the amphibian egg, then that of the mammalian egg, and finally that of the mammalian embryonic stem cell. Most recently, the demonstration that a limited pool of defined molecules is capable of reprogramming a multitude of cell types has provided massive impetus and facilitated transition towards realistic therapeutic application. We have therefore reproduced some of the key articles that elegantly document these dramatic stages of development of the field in an inclusive appendix to the book, for the benefit of readers keen to investigate the history of how the field of stem cell biology has evolved. Owing to the ever broadening nature of this field, and the incredible rate at which it is evolving, the main content of this volume focuses on areas that have shown significant movement in recent years, are most likely to translate into personalized therapeutic application, and thus provide greatest potential for significant impact on human health in the not too distant future. We recognize that research into many other disease states and cell types are all equally worthy of discussion. We would therefore like to acknowledge those researchers involved whose work we have not been able to include in this volume. Nuclear Reprogramming and Stem Cells will serve as a valuable resource for all researchers in the field of stem cell biology, including those just setting out on their career path as well as those already established in the field.
  • 2006 Springer Protocols
    edited by Paul J. Verma, Alan O. Trounson.
    Cloning of exotic/endangered species: (desert bighorn sheep) / James "Buck" Williams ... [et al.] -- Handmade somatic cell cloning in cattle / Gábor Vatja, Ian M. Lewis, and R. Tayfur Tecirlioglu -- Genetic modification of sheep by nuclear transfer with gene-targeted somatic cells / A. John Clark, Sarah Burl, and Chris Denning -- Transgenesis using nuclear transfer in goats / Anthoula Lazaris ... [et al.] -- Nuclear reprogramming : an overview / Takashi Tada -- Expression of imprinted genes in cloned mice / Takashi Kohda, Fumitoshi Ishino, and Atsuo Ogura -- Nuclear remodeling assay in xenopus egg extract / Koichi Gonda and Nobuaki Kikyo -- In vitro reprogramming of nuclei and cells / Anne-Mari Håkelien ... [et al.] -- Techniques for nuclear transfer to mouse embryonic stem cells / Danielè Pralong and Paul J. Verma -- Gene expression and methylation patterns in cloned embryos / Christine Wrenzycki ... [et al.] -- Are there any normal clones? / Ian Wilmut -- Future and applications of cloning / Alan O. Trounson. Origin and progress of nuclear transfer in nonmammalian animals / Marie A. Di Berardino -- In vitro maturation and embryo production in cattle / Marc-André Sirard and Karine Coenen -- Activation of oocytes after nuclear transfer / Zoltán Macháty -- Culture medium preferences of pre-implantation cloned mouse embryos / Björn Heindryckx, Josiane Van der Elst, and Marc Dhont -- Maintenance of pregnancy in pigs with limited viable embryos / Tim King and Paul A. De Sousa -- Health and neonatal care of bovine clones / Jacqui T. Forsyth and David N. Wells -- Optimization of procedures for cloning by somatic cell nuclear transfer in mice / Young Gie Chung, Shaorong Gao, and Keith E. Latham -- Production of cloned calves using roscovitine-treated adult somatic cells as donors / Kazuchika Miyoshi, Sezen Arat, and Steven L. Stice -- A double nuclear transfer technique for cloning pigs / Irina A. Polejaeva, Shawn Walker, and Keith Campbell -- Nuclear transfer in nonhuman primates / Shoukhrat M. Mitalipov and Don P. Wolf --
  • 2014 Springer
    Jørgen Kjems, Elena Ferapontova, Kurt V. Gothelf, editors.
    This volume on nucleic acid nanotechnology offers authoritative, up-to-date and comprehensive coverage of nanotechnological studies and applications of nucleic acids. It provides reviews of various aspects of nucleic acid nanotechnology, each written by an internationally leading expert in the field, and presents state-of-the-art and recent advances in nucleic acid synthetic modifications, nanoscale design, manipulation and current and future applications in bioengineering, medicine, electronics, genetic analysis, chemistry, molecular biology, surface and material sciences. It examines how nucleic acid research is merging with nanotechnology, allowing the nanoscale properties of nucleic acid to be exploited in performing challenging nanotechnological tasks, from nanorobotics and nanosensing to nucleic acid computing. This book will above all benefit anyone who is interested in nanotechnological concepts of nucleic acid design and applications, and offers a valuable resource for teaching these concepts. It is essential reading for a broad audience of scientists both in academia and industry who wish to expand their expertise on the potential of nucleic acid functions and applications.
  • 2014 Springer
    Katsuhiko S. Murakami, Michael A. Trakselis, editors.
    This book provides a review of the multitude of nucleic acid polymerases, including DNA and RNA polymerases from Archea, Bacteria and Eukaryota, mitochondrial and viral polymerases, and other specialized polymerases such as telomerase, template-independent terminal nucleotidyl transferase and RNA self-replication ribozyme. Although many books cover several different types of polymerases, no book so far has attempted to catalog all nucleic acid polymerases. The goal of this book is to be the top reference work for postgraduate students, postdocs, and principle investigators who study polymerase.
  • 2011 Springer
    Mark O.J. Olson [editor].
  • 2015 Springer Protocols
    edited by Ronald Hancock.
    Cell type-specific affinity purification of nuclei for chromatin profiling in whole animals / Florian A. Steiner and Steven Henikoff -- Lysis gradient centrifugation : a flexible method for the isolation of nuclei from primary cells / Karl Katholnig ... [et al.] -- Isolation of nuclei in media containing an inert polymer to mimic the crowded cytoplasm / Ronald Hancock and Yasmina Hadj-Sahraoui -- New rapid method for isolating nucleoli / Zhou Fang Li and Yun Wah Lam -- Sequential recovery of macromolecular components of the nucleolus / Baoyan Bai and Marikki Laiho -- Au nanoinjectors for electrotriggered gene delivery into the cell nucleus / Mijeong Kang and Bongsoo Kim -- Improving chromatin immunoprecipitation (ChIP) by suppression of method-induced DNA-damage signaling / Sascha Beneke -- Purification of specific chromatin loci for proteomic analysis / Stephanie D. Byrum, Sean D. Taverna, and Alan J. Tackett -- Chromatin structure analysis of single gene molecules by psoralen cross-linking and electron microscopy / Christopher R. Brown ... [et al.] -- Purification of proteins on newly synthesized DNA using iPOND / Huzefa Dungrawala and David Cortez -- Applying the ribopuromycylation method to detect nuclear translation / Alexandre David and Jonathan W. Yewdell -- Targeted nano analysis of water and ions in the nucleus using cryo-correlative microscopy / Frédérique Nolin ... [et al.] -- Redox-sensitive yellow fluorescent protein sensor for monitoring nuclear glutathione redox dynamics / Agata Banach-Latapy, Michèle Dardalhon, and Meng-Er Huang -- Determination of the dissociation constant of the NF[Kappa]B p50/p65 heterodimer in living cells using fluorescence cross-correlation spectroscopy / Manisha Tiwari and Masataka Kinjo -- Imaging and quantification of amyloid fibrillation in the cell nucleus / Florian Arnhold, Andrea Scharf, and Anna von Mikecz -- Analysis of nuclear organization with TANGO, software for high-throughput quantitative analysis of 3D fluorescence microscopy images / Jean Ollion ... [et al.] -- Quantitative analysis of chromosome localization in the nucleus / Sandeep Chakraborty ... [et al.].
  • 2005 CRCnetBASE
    edited by Janos Zempleni, Krishnamurti Dakshinamurti.
    Also available: Print – 2005
  • 2012 Springer Protocols
    edited by Chrissa Kioussi.
    Histological analysis of the embryonic and adult tooth / Atsushi Ohazama -- Determination of gene expression patterns by whole-mount in situ hybridization / Sergiy Kyryachenko [and others] -- Determination of gene expression patterns by in situ hybridization in sections/ Kateryna Kyrylkova [and others] -- Immunohistochemistry and detection of proliferating cells by BrdU / Sergiy Kyryachenko [and others] -- Detection of apoptosis by TUNEL assay / Kateryna Kyrylkova [and others] -- Use of siRNA in dental tissue-derived cell cultures : integrin knockdown in fibroblasts / Malgorzata M. Barczyk, Donald Gullberg, and Anne Isine Bolstad -- Organ cultures and kidney-capsule grafting of tooth germs / Keishi Otsu, Naoki Fujiwara, and Hidemitsu Harada -- Evaluation of skull and tooth morphology and mineralization using high-resolution x-ray tomography / Brian K. Bay -- Electron microscopy / Hans U. Luder and Margrit Amstad-Jossi -- Deoxyoligonucleotide microarrays for gene expression profiling in murine tooth germs / Harald Osmundsen, Anne-Marthe Jevnaker, and Maria A. Landin -- Lineage differentiation of mesenchymal stem cells from dental pulp, apical papilla, and periodontal ligament / Kentaro Akiyama [and others] -- In vivo transplantation and tooth repair / Shuhei Tsuchiya and Masaki J. Honda -- Methods to validate tooth-supporting regenerative therapies / Miguel Padial-Molina [and others] -- Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (organ germ method) / Masamitsu Oshima [and others] -- In vitro studies on odontogenic tumors / Javier Caton, Thimios A. Mitsiadis, and Peter R. Morgan -- Whole mount immunohistochemistry and in situ hybridization of larval and adultzZebra fish dental tissues / Barbara Verstraeten, Ellen Sanders, and Ann Huysseune.
  • 2013 Springer
    S. Samuel Kim, editor.
    Fertility preservation has become one of the most important and fast growing fields of reproductive medicine. Although there are several strategies for fertility preservation in female, most of them are still considered experimental. It is important to perfect the existing technologies, but also developing new strategies should be actively sought. The future development of fertility preservation strategies should be based on the sound scientific knowledge and principles. One of the main objectives of fertility preservation in females is prevention of oocyte depletion. The mechanisms of oocyte loss and survival in the ovary are complex, which include genetic control both in germ cells and in somatic cells, DNA damage and repair mechanism, apoptosis and autophagy, and other poorly understood molecular mechanisms.To develop clinically effective and safe strategies for fertility preservation, it is essential to know and understand the fundamentals of oocyte and ovarian biology at the molecular level. Thus, the purpose of this edition is to review the current progress in research related to molecular and genetic control of oocyte development that can be applied to fertility preservation. The main topics that are discussed in this publication include molecular signaling mechanisms of oocyte activation and loss, genomic integrity of oocytes, and epigenetics.
    Also available: Print – 2013
  • 2013 Springer
    Giovanni Coticchio, David F. Albertini, Lucia De Santis, editors.
    The oocyte is the largest and most mysterious cell in the body of mammalian organisms. Through its growth and maturation, it reaches extreme levels of specialization, while maintaining at the same time a condition of totipotency. Its unique ability, in co-operation with the spermatozoon, to give rise to a fully developed organism formed from hundreds of different tissues and myriads of individual cells has inspired intellectuals of all ages.Oogenesis finds impetus and purpose in casting scientific perspective towards this unique cell for the benefit of scientists and assisted reproductive technology (ART) specialists. The authors of the chapters are distinguished authorities in their respective areas of competence. From the opening of the book the reader is lead on a fantastic voyage from the formation of the primordial oocyte to the development of the early embryo, passing through crucial processes of oogenesis, such as co-ordination of oocyte and follicle growth, gene expression and organelle reorganization during growth and maturation, epigenetic mechanisms, regulation of meiosis, totipotency, cell polarity, oogenesis in vitro and maternal regulation of early development.
  • 2011 Springer
    Alberto Diaspro, editor.
    Fundamentals of optical microscopy / Franco Quercioli -- The white confocal: continuous spectral tuning in excitation and emission / Rolf Borlinghaus -- Second/third harmonic generation microscopy / Shakil Rehman ... [et al.] -- Role of scattering and nonlinear effects in the illumination and the photobleaching distribution profiles / Zeno Lavagnino, Francesca Cella Zanacchi and Alberto Diaspro -- New analytical tools for evaluation of spherical aberration in optical microscopy / Isabel Escobar ... [et al.] -- Improving image formation by pushing the signal-to-noise ratio / Emiliano Ronzitti ... [et al.] -- Site-specific labeling of proteins in living cells using synthetic fluorescent dyes / Gertrude Bunt -- Imaging molecular physiology in cells using FRET-based fluorescent nanosensors / Fred S. Wouters -- Measuring molecular dynamics by FRAP, FCS, and SPT / Kevin Braeckmans ... [et al.] -- In vitro-in vivo fluctuation spectroscopies / M. Collini ... [et al.] -- Interference x-ray diffraction from single muscle cells reveals the molecular basis of muscle braking / L. Fusi ... [et al.] -- Low concentration protein detection using novel SERS devices / Gobind Das ... [et al.] -- Near infrared three-dimensional nonlinear optical monitoring of stem cell differentiation / Uday K. Tirlapur and Clarence Yapp -- A correlative microscopy: a combination of light and electron microscopy / Umberto Fascio and Anna Sartori-Rupp.
  • 2007 CRCnetBASE
    Guy Cox.
  • 2010
    Robert Barretto.
    Optical microendoscopy is an emerging modality for imaging in live subjects. Using gradient refractive index (GRIN) microlenses, microendoscopy enables subcellular-resolution imaging in deep tissues that are inaccessible by traditional imaging techniques. We present a platform of methods and technologies that build upon GRIN microendoscopy: 1) miniaturized microscopes for imaging in awake, behaving animals, 2) methods for imaging contractile dynamics in the muscles of animal and human subjects, 3) chronic brain preparations that allow for longitudinal examinations of subcellular neuronal features and disease progression, and 4) novel microendoscope probes whose imaging capabilities approach that of standard water-immersion microscope objectives. When combined with the broad sets of available fluorescent reporters, and minimally invasive surgical preparations, the work described in this dissertation enables sophisticated experimental designs for probing how cellular characteristics may underlie or explain behavior, in models of both healthy and diseased states.
  • 2013 Springer
    Ravi Mehrotra, editor.
    Oral Cytology: A Concise Guide provides a state-of-the-art review of the major issues specific to the field of oral cytology. The sections of the book have been structured to review the complexities of sample collection, manual/automated analysis, pitfalls, gray zones and controversies in diagnosis, as well as, issues not covered by other available materials. Recent advancements in ancillary techniques like DNA ploidy, image analysis and molecular biology are discussed in detail. This useful text is extensively illustrated with patient and microscopic pictures, which will further highlight the clinical application. Throughout the text, simultaneous inclusion of both cytologic and histologic images will allow cyto-histological correlation. Readers will find descriptions of techniques offering greater simplicity, speed, patient comfort and cost effectiveness, as well as improved diagnostic precision. This bench-side reference is a unique and valuable resource in the field of oral cytology, both for those currently in training and clinicians dealing with the diagnosis of oral lesions.
  • 2012 ScienceDirect
    2012 ClinicalKey
    [edited by] Svante R. Orell, Gregory F. Sterrett.
    Chapter 1 Introduction -- Chapter 2 The techniques of FNA cytology -- Chapter 3 Imaging methods for guidance of aspiration cytology -- Chapter 4 Head and neck; salivary glands -- Chapter 5 Lymph nodes -- Chapter 6 The thyroid gland -- Chapter 7 Breast -- Chapter 8 Lung, chest wall and pleura -- Chapter 9 Mediastinum -- Chapter 10 Liver and spleen -- Chapter 11 Pancreas, biliary tract and intra-abdominal organs -- Chapter 12 Kidney, adrenal and retroperitoneum -- Chapter 13 Male and female genital organs -- Chapter 14 Skin and subcutis -- Chapter 15 Soft tissues -- Chapter 16 Bone and cartilage -- Chapter 17 Paediatric tumors -- Chapter 18 Infectious Diseases.
  • 2011
    Daniel James Dickinson.
    A fundamental characteristic of metazoans is the formation of a simple, polarized epithelium. In higher animals, the structural integrity and functional polarization of simple epithelia require a cell-cell adhesion complex containing a classical cadherin, the Wnt-signaling protein [Beta]-catenin and the actin-binding protein [Alpha]-catenin. I have investigated the evolutionary origins of epithelial cell polarity and of the cadherin-catenin complex. I show that the non-metazoan Dictyostelium discoideum forms a polarized epithelium that is essential for multicellular development. Although D. discoideum lacks a cadherin homolog, I have identified and characterized an [Alpha]-catenin ortholog that binds a [Beta]-catenin-related protein. Both proteins are essential for formation of the epithelium, polarized protein secretion and proper multicellular morphogenesis. Thus the organizational principles of metazoan multicellularity may be more ancient than previously recognized, and the role of the catenins in cell polarity predates the evolution of Wnt signaling and classical cadherins.
  • 2006 Springer
    Olivier Civelli, Qun-Yong Zhou (eds.).
    Also available: Print – 2008
  • 2007 ScienceDirect
    edited by Dieter Häussinger, Helmut Sies.
    Water compartments in cells / G. D. Fullerton ... [et al.] -- Yeast osmoregulation / S. Hohmann ... [et al.] -- Physiological analysis of bacterial mechanosensitive channels / I. R. Booth ... [et al.] -- Control of cell cycle in response to osmostress: Lessons from yeast / J. Clotet ... [et al.] -- Bacterial osmosensing transporters / J. M. Wood -- Plant gene networks in osmotic stress response: From genes to regulatory networks / L. S. Tran ... [et al.] -- Osmosensing by integrins in rat liver / F. Schliess ... [et al.] -- Hyperosmotic activation of the CD95 system / R. Reinehr ... [et al.] -- New approaches for determining apoptotic volume decrease in cells / C. D. Bortner ... [et al.] -- Transient receptor potential channels in mechanosensing and cell volume regulation / S. F. Pedersen ... [et al.] -- Cell volume regulatory ion channels in cell proliferation and cell death / F. Lang ... [et al.] -- Actin cytoskeleton architecture and signaling in osmosensing / E. A. Papakonstanti ... [et al.] -- Osmotic stress and DNA damage / N. I. Dmitrieva ... [et al.] -- Transcriptional activator tone-binding protein in cellular protection and differentiation / J. A. Kim ... [et al.] -- Desiccation response of mammalian cells: Anhydrosignaling / Z. Huang ... [et al.] -- Tonicity-regulated gene expression / J. D. Ferraris ... [et al.] -- Hyperosmotic induction of mitogen-activated protein kinase scaffolding / T. L. Hilder ... [et al.] -- Osmoregulation of bile formation / R. Kubitz ... [et al.] -- Osmosignaling and volume regulation in intestinal epithelial cells / C. H. Lim ... [et al.] -- Osmotic regulation of cellular glucose uptake / P. Gual ... [et al.] -- Effects of osmolytes on protein folding and aggregation in cells / Z. Ignatova ... [et al.] -- Simulations of macromolecules in protective and denaturing osmolytes: Properties of mixed solvent systems and their effects on water and protein structure and dynamics / D. A. Beck ... [et al.] -- Application of the transfer model to understand how naturally occurring osmolytes affect protein stability / M. Auton ... [et al.] -- Mechanisms of high salinity tolerance in plants / N. Tuteja -- Phenotype of the taurine transporter knockout mouse / U. Warskulat ... [et al.] -- Molecular basis of osmolyte effects on protein and metabolites / J. Rosgen -- Methods of changing biopolymer volume fraction and cytoplasmic solute concentrations for in vivo biophysical studies / M. C. Konopka ... [et al.] -- Characterization of plant aquaporins / R. Kaldenhoff ... [et al.].
    Also available: Print – 2007
  • 2012
    Scott Fraser Owen.
    Neural circuits throughout the brain are under the continuous influence of neuromodulators which shape network activity in accordance with behavioral context. Oxytocin is a key neuromodulator that has been linked to social memory and maternal behavior in animals, as well as to autism spectrum disorders, trust, emotion recognition and parenting in humans. Here we show that activation of oxytocin receptors sharpens the responses of the hippocampal circuit, increasing the signal of spike transmission through the network while simultaneously suppressing the noise of background spontaneous activity. Both of these actions are mediated through a depolarization of the fast-spiking interneurons. The resulting increase in inhibitory tone serves to silence spontaneous activity in the CA1 pyramidal cells, while a use-dependent depression of the inhibitory synapses permits enhanced feed-forward spike transmission. Furthermore, we show that oxytocin potently modulates spontaneous hippocampal Sharp-Wave Ripple oscillations in a slice preparation. These results elucidate the action of oxytocin in the hippocampus, while simultaneously shedding light on a novel mechanism by which modulation of fast-spiking interneurons can modify hippocampal circuit activity.
  • 2011
    Colleen Ann Brady.
    The p53 protein suppresses tumorigenesis by inducing cell cycle arrest, apoptosis, or cellular senescence. At the molecular level p53 plays a central role as a transcriptional activator, and p53 has two amino-terminal transactivation domains important for this role. However, the contribution of each of these domains to p53 activity has not been determined in vivo. To analyze transactivation domain requirements for p53 function, we have generated a series of p53 knock-in mouse models in which we replaced the endogenous p53 gene with transactivation mutants containing point mutations in the first (p53^25,26), second (p53^53,54), or both (p53^25,26,53,54) p53 transactivation domains. These experiments are the first to address the unique contribution of each of the p53 transactivation domains in vivo. By gene expression microarray, we determined that these mutants represent an allelic series of p53 transactivation mutants, whereby p53^53,54 retains wild-type p53 transactivation potential, p53^25,26,53,54 loses nearly all p53-dependent transactivation, and p53^25,26 exhibits a severely compromised transactivation phenotype. Experiments comparing all three transactivation domain mutants in cell culture and mouse models have unveiled important differences in the function of the two transactivation domains. Apoptosis or cell-cycle arrest downstream of acute DNA damage rely on the first transactivation domain and robust p53 transactivation, while senescence and tumor suppression in response to oncogene activation can occur without the full transactivation function of p53. These results implicate unique p53 transcriptional programs in acute DNA damage responses and tumor suppression, and mechanistically distinguish these two p53 response pathways. Further, we have uncovered striking differences in the developmental phenotypes of these different mutant mice, leading to conclusions about the activity of p53 tetramers.
  • 2009 Springer
    Juan Domínguez-Bendala.
  • 2012
    Samuel Bandara.
    Cell signaling networks are complex dynamical systems with mechanisms for adaptation and failure-tolerance such that typical stimulus experiments are often robust to the perturbation of individual components. While such robustness is often advantageous, it also means that the information content of an experiment with respect to internal states and activities is often poor. We treat this problem formally by examining the statistical uncertainty of parameter estimates of corresponding signaling models as a function of experimental protocols that are based on live-cell imaging of fluorescent reporters. The first part of this work uses such model predictions of parameter sensitivity to design numerically optimized live-cell imaging protocols with the goal to maximally constrain the space of possible parameter values. The second part instead uses an experimental approach to define invariant parameters by correlated population estimates in order to reveal changes of regulated parameters in systematic siRNA perturbation experiments. Our results show that the effects of siRNA perturbations on reporter dynamics can be used to infer functional changes of the probed system. We exploit this feature to define not only the key molecules responsible for activity but also direct and indirect regulators.
  • 2016 Springer
    Dorothy L. Rosenthal, Eva M. Wojcik, Daniel F.I. Kurtycz, editors.
    Pathogenesis of Urothelial Carcinoma -- Adequacy of Urine Specimens (Adequacy) -- Negative for High Grade Urothelial Carcinoma (Negative) -- Atypical Urothelial Cells (AUC) -- Suspicious for High Grade Urothelial Carcinoma (Suspicious) -- High Grade Urothelial Carcinoma (HGUC) -- Low Grade Urothelial Neoplasia (LGUN) -- Other Malignancies Primary and Metastatic and Miscellaneous Lesions -- Ancillary Studies in Urinary Cytology -- Cytopreparatory Techniques -- Clinical Management, Including Microscopic Hematuria.
  • 2015 Springer
    Ellen Hsu, Louis Du Pasquier.
    Evolution of immunity and pathogens -- A host-pathogen interaction reduced to first principles: Antigenic variation in T. brucei -- Antigenic variation in Plasmodium falciparum -- Polymorphic mucin-like proteins in Schistosoma mansoni, a variable antigen and a key component of the compatibility between the schistosome and its snail host -- Fibrinogen-related proteins (FREP) in mollusks -- Somatic and germline diversification of a putative immunoreceptor within one phylum: Dscam in arthropods -- An immune effector system in the Protochordate gut sheds light on fundamental aspects of vertebrate immunity -- Variable lymphocyte receptors: a current view -- Antibody repertoire in fish -- Unique features of fish immune repertoires: particularities of the adaptive immunity within the largest group of vertebrates -- The evolution and structure of atypical T cell receptors -- Diversification of the primary antibody repertoire by AID-mediated gene conversion -- Antibody isotype switching in vertebrates.
    Also available: Print – 2015
  • 2016 Springer Protocols
    edited by Andras Nagy, Kursad Turksen.
    Generation of patient-specific induced pluripotent stem cell from peripheral blood mononuclear cells by Sendai reprogramming vectors / Oscar Quintana-Bustamante and Jose C. Segovia -- Doxycycline-inducible system for genetic correction of iPSC disease models / Xiuli Sim ... [et al.] -- Generation and characterization of patient-specific induced pluripotent stem cell for disease modeling / Renuka Sivapatham and Xianmin Zeng -- Modeling genomic imprinting disorders using induced pluripotent stem cells / Stormy J. Chamberlain ... [et al.] -- Generation and characterization of induced pluripotent stem cells from patients with mtDNA mutations / Riikka H. Hssamssalssainen and Anu Suomalainen -- Skin biopsy and patient-specific stem cell lines / Yao Li, Huy V. Nguyen, and Stephen H. Tsang -- Directed myogenic differentiation of human induced pluripotent stem cells / Emi Shoji, Knut Woltjen, and Hidetoshi Sakurai -- Using human induced pluripotent stem cells to model skeletal diseases / Emilie Barruet and Edward C. Hsiao -- Modeling cardiovascular diseases with patient-specific human pluripotent stem cell-derived cardiomyocytes / Paul W. Burridge ... [et al.] -- Calcium imaging in pluripotent stem cell-derived cardiac myocytes / Anna Walter ... [et al.] -- Patient-specific induced pluripotent stem cell models : generation and characterization of cardiac cells / Fabian Zanella and Farah Sheikh -- Differentiation of human pluripotent stem cells to cardiomyocytes under defined conditions / Cathelijne W. van den Berg ... [et al.] -- Generation of cardiomyocytes from pluripotent stem cells / Hiroko Nakahama and Elisa Di Pasquale -- Generation and characterization of patient-specific iPSC model for cardiovascular disease / Yee Ki Lee ... [et al.] -- Transgene-free disease-specific iPSC generation from fibroblasts and peripheral blood mononuclear cells / Kerem Fidan ... [et al.] -- Generation and neuronal Differentiation of patient-specific induced pluripotent stem cells derived from Niemann-Pick type C1 fibroblasts / Michaela Trilck, Rayk Hubner, and Moritz J. Frech -- Multisystemic disease modeling of liver-derived protein folding disorders using induced pluripotent stem cells (iPSCs) / Amy Leung and George J. Murphy -- In vitro modeling of alcohol-induced liver injury using human-induced pluripotent stem cells / Lipeng Tian, Neha Prasad, and Yoon-Young Jang -- Generation of human induced pluripotent stem cells using RNA-based sendai virus system and pluripotency validation of the resulting cell population / Valeria Chichagova ... [et al.] -- Modeling axonal phenotypes with human pluripotent stem cells / Kyle R. Denton, Chong-Chong Xu, and Xue-Jun Li -- Mitochondrial disease-specific induced pluripotent stem cell models : generation and characterization / Xuan Zhang ... [et al.] -- Patient-specific induced pluripotent stem cell models : characterization of iPS cell-derived cardiomyocytes / Toru Egashira ... [et al.] -- Generation of integration-free patient specific iPS cells using episomal plasmids under feeder free conditions / Sara Caxaria ... [et al.].
  • 2013 Wiley
    edited by Kyle J. Cetrulo [and others].
    Perinatal Stem Cells, 2nd Edition builds on the first edition to provide an updated tutorial on perinatal stem cells, including stem cells harvested from the amniotic fluid, placenta, maternal blood supply, umbilical cord and Wharton's Jelly. As in the first edition, coverage includes the underlying biology of each of the sources of pregnancy related stem cells, cell culture, and potential therapeutic uses, as well as insights on the impact of these stem cells from obstetricians and gynecologists, cardiologists, hematologists, tissue engineers, and cord blood bankers. Normally discard.
  • 2011 Springer Protocols
    edited by Kursad Turksen.
    Computational modeling of the skin barrier / Arne Naegel, Michael Heisig, and Gabriel Wittum -- In vitro human skin segmentation and drug concentration-skin depth profiles / Ana Melero [and others] -- Transcriptional regulation of epidermal barrier formation / Ambica Bhandari [and others] -- Epidermal permeability barrier measurement in mammalian skin / Arup Kumar Indra and Mark Leid -- Assessment of permeability barriers to macromolecules in the rodent endometrium at the onset of implantation / Brent M. Bany and G. Scot Hamilton -- Assessment of intestinal permeability in (premature) neonates by sugar absorption tests / Willemijn E. Corpeleijn [and others] -- Analysis of epithelial cell shedding and gaps in the intestinal epithelium / Carrie A. Duckworth and Alastair J. Watson -- Studying permeability in a commonly used epithelial cell line : T84 intestinal epithelial cells / Rino P. Donato [and others] -- Optimization of the caco-2 permeability assay to screen drug compounds for intestinal absorption and efflux / Barry Press -- Ouabain modulates cell contacts as well as functions that depend on cell adhesion / Isabel Larre, Ruben G. Contreras, and Marcelino Cereijido -- Monitoring of the dynamics of epithelial dome formation using a novel culture chamber for long-term continuous live-cell imaging / Judith Lechner [and others] -- Measuring permeability in human retinal epithelial cells (ARPE-19) : implications for the study of diabetic retinopathy / Marta Garcia-Ramirez [and others] -- Analysis of epithelial barrier integrity in polarized lung epithelial cells / Monika Strengert and Ulla G. Knaus -- Permeability of differentiated human urothelium in vitro / Peter Rubenwolf and Jennifer Southgate -- Phenotyping the claudin 11 deficiency in testis : from histology to immunohistochemistry / Severine Mazaud-Guittot, Alexander Gow, and Brigitte Le Magueresse-Battistoni -- In vitro system to study sertoli cell blood-testis barrier dynamics / Dolores D. Mruk and C. Yan Cheng -- Analysis of endothelial barrier function in vitro / Yuping Wang and J. Steven Alexander -- Role of endothelial cell-cell junctions in endothelial permeability / Armelle Le Guelte and Julie Gavard -- In vitro analyses of endothelial cell permeability / Elizabeth Monaghan-Benson and Erika S. Wittchen -- Mechano-transduction and barrier regulation in lung microvascular endothelial cells / Kristina Giantsos, Mark Cluff, and Randal Dull -- Role of Caveolin-1 in the regulation of pulmonary endothelial permeability / Yu Sun, Richard D. Minshall, and Guochang Hu -- Assessment of endothelial permeability and leukocyte transmigration in human endothelial cell monolayers / Andreas Ludwig, Anselm Sommer, and Stefan Uhlig -- Permeability of endothelial Barrier : cell culture and in vivo models / Alexander N. Garcia [and others] -- Size-selective and in vitro assessment of inner blood retina barrier permeability / Matthew Campbell and Peter Humphries -- Assessment of permeability in barrier type of endothelium in brain using tracers : evans blue, sodium fluorescein, and horseradish peroxidase / Mehmet Kaya and Bulent Ahishali -- In vitro and in vivo methods for assessing FcRn-mediated reverse transcytosis across the blood-brain barrier / Nadia Caram-Salas [and others] -- Evaluation of VEGF-induced vascular permeability in mice / Sara M. Weis -- In vivo measurement of glioma-induced vascular permeability / Jisook Lee, Andrew Baird, and Brian P. Eliceiri -- In vivo optical imaging of ischemic blood-brain barrier disruption / Abedelnasser Abulrob [and others].
  • 2007 Springer
    edited by Leopold Flohé and J. Robin Harris.
    Introduction / Leopold Flohé and J. Robin Harris -- Evolution of the peroxiredoxins / Bernard Knoops, Eléonore Loumaye and Valérie Van Der Eecken -- Structural survey of the peroxiredoxins / P. Andrew Karplus and Andrea Hall -- The catalytic mechanism of peroxiredoxins / Leslie B. Poole -- Kinetics of peroxiredoxins and their role in the decomposition of peroxynitrite / Madia Trujillo ... [et al.] -- The peroxiredoxin repair proteins / Thomas J. Jönsson and W. Todd Lowther -- Peroxiredoxins in bacterial antioxidant defense / James M. Dubbs and Skorn Mongkolsuk -- The NADH oxidase-Prx system in Amphibacillus xylanus / Youichi Niimura -- Peroxiredoxin systems in mycobacteria / Timo Jaeger -- Peroxiredoxin systems of protozoal parasites / Marcel Deponte, Stefan Rahlfs and Katja Becker -- The trypanothione system / Luise R. Krauth-Siegel, Marcelo A. Comini and Tanja Schlecker -- Functions of typical 2-Cys peroxiredoxins in yeast / Brian A. Morgan and Elizabeth A. Veal -- The dual function of plant peroxiredoxins in antioxidant defence and redox signaling / Karl-Josef Dietz -- Mitochondrial peroxiredoxins / Zhenbo Cao, J. Gordon Lindsay and Neil W. Isaacs -- Peroxiredoxins in the lung with emphasis on peroxiredoxin VI / Bruno Schremmer ... [et al.] -- Peroxiredoxins in gametogenesis and embryo development / Isabelle Donnay and Bernard Knoops -- Peroxiredoxins in the central nervous system / Fumiyuki Hattori and Shinzo Oikawa -- Stress-induced peroxiredoxins / Tetsuro Ishii and Toru Yanagawa.
  • 2013 Springer
    Luis A. del Río, editor.
    Peroxisomes are a class of ubiquitous and dynamic single membrane-bounded cell organelles, devoid of DNA, with an essentially oxidative type of metabolism. In recent years it has become increasingly clear that peroxisomes are involved in a range of important cellular functions in almost all eukaryotic cells. In higher eukaryotes, including humans, peroxisomes catalyze ether phospholipids biosynthesis, fatty acid alpha-oxidation, glyoxylate detoxification, etc, and in humans peroxisomes are associated with several important genetic diseases.
  • 2010 Springer
    Henning Ulrich, editor.
  • 2009 Springer
    Dmitri V. Krysko, Peter Vandenabeele, editors.
  • 2013 Springer Protocols
    edited by Kazushige Touhara.
    Female moth pheromones / Tetsu Ando -- Pheromones in the fruit fly / Jacqueline S.R. Chin and Joanne Y. Yew -- Analysis of volatile mouse pheromones by gas chromatography mass spectrometry / Milos V. Novotny and Helena A. Soini -- Murine nonvolatile pheromones : isolation of exocrine-gland secreting peptide 1 / Hiroko Kimoto and Kazushige Touhara -- Chemical analysis of aquatic pheromones in fish / Michael Stewart, Cindy F. Baker, and Peter W. Sorensen -- Analysis of ascarosides from Caenorhabditis elegans using mass spectrometry and NMR spectroscopy / Xinxing Zhang ... [et al.] -- Identification of chemosensory receptor genes from vertebrate genomes / Yoshihito Niimura -- Functional assays for insect olfactory receptors in Xenopus oocytes / Tatsuro Nakagawa and Kaushige Touhara -- A protocol for heterologous expression and functional assay for mouse pheromone receptors / Sandeepa Dey, Senmiao Zhan, and Hiroaki Matsunami -- Genetic manipulation to analyze pheromone responses : knockouts of multiple receptor genes / Tomohiro Ishii -- Electroantennogram and single sensillum recording in insect antennae / Shannon B. Olsson and Bill S. Hansson -- Calcium imaging of pheromone responses in the insect antennal lobe / Susy M. Kim and Jing W. Wang -- Live cell calcium imaging of dissociated vomeronasal neurons / Angeldeep Kaur, Sandeepa De, and Lisa Stowers -- Whole-mount imaging of responses in mouse vomeronasal neurons / Pei Sabrina Xu and Timothy E. Holy -- Calcium imaging of vomeronasal organ response using slice preparations from transgenic mice expressing G-CaMP2 / C. Ron Yu -- The electrovomeronasogram : field potential recordings in the mouse vomeronasal organ / Trese Leinders-Zufall and Frank Zufall -- Electrical recordings from the accessory olfactory bulb in VNO-AOB ex vivo preparations / Julian P. Meeks and Timothy E. Holy -- Pheromone -induced expression of immediate early genes in the mouse vomeronasal sensory system / Sachiko Haga-Yamanaka and Kazushige Touhara -- Insect pheromone behavior : fruit fly / Daisuke Yamamoto, Soh Kohatsu, and Masayuki Koganezawa -- Quantitative assessment of pheromone-induced dauer formation in Caenorhabditis elegans / Scott J. Neal, Kyuhyung Kim, and Piali Sengupta -- Acute behavioral responses to pheromones in C. elegans (adult behaviors : attraction, repulsion ) / Heeun Jang and Cornelia I. Bargmann -- Behavioral analysis of pheromones in fish / Peter W. Sorensen -- Analysis of male aggressive and sexual behavior in mice / Takefumi Kikusui -- Assessment of urinary pheromone discrimination, partner preference, and mating behaviors in female mice / Olivier Brock, Julie Bakker, and Michael J. Baum.
  • 2014 Springer
    Paramjit S. Tappia, Naranjan S. Dhalla, editors.
    Phospholipases generate lipid signaling molecules through their hydrolytic action on phospholipids and are known to regulate function of a variety of cells under normal and diseased conditions. While several physiological, biochemical and molecular techniques have identified key players involved in different disease processes, phospholipases have also emerged as critical players in the pathogenesis of a number of different diseases including cancer and heart disease. In addition, phospholipases are also implicated in such conditions as brain disorder/injury, kidney and immune cell dysfunction. Phospholipases in Health and Disease is a compilation of review articles dedicated to the study of the field with respect to biochemical and molecular mechanisms of normal and abnormal cell function. The wide range of area covered here is of interest to basic research scientists, clinicians and graduate students, who are engaged in studying pathophysiological basis of a variety of diseases. Furthermore, this book highlights the potential of the differ