Books by Subject

Cell Biology

  • Digital
    [edited by] P. Michael Conn.
    ScienceDirect2013
    Structural dynamics of nucleic acids by single-molecule FRET / Asger Christian Krüger, Lasse Lava Hildebrandt, Sofie Louise Kragh, Victoria Birkedal -- Visualizing the location and the dynamics of gene expression in living animals through bioluminescence imaging / Xiaojuan Chen -- Quality control protocol for confocal system / Anda Cornea -- Electron microscopy of bone / V. Everts, K.A. Hoeben -- Immunostaining : detection of signaling protein location in tissues, cells and subcellular compartments / Biswanath Maity, David Sheff, Rory A. Fisher -- Bimolecular fluorescence complementation (BiFC) analysis of protein-protein interaction : how to calculate signal-to-noise ratio / Yutaka Kodama, Chang-Deng Hu -- Immunohistochemical detection of neuropeptides/transmitters in mammalian brain / Lothar Jennes -- Intravital imaging of autoreactive T. cells in living animals / Naoto Kawakami, Ingo Bartholomäus, Marija Pesic, Nikolaos I. Kyratsous -- Genetically encoded click chemistry for single-molecule FRET of proteins / Swati Tyagi, Edward A. Lemke -- High-throughput methods for the detection of protein overexpression using fluorescence markers / Mads Gabrielsen, Andrew J. Roe, Karen McLuskey -- Correlative microscopy / Alexander A. Mironov, Galina V. Beznoussenko -- Stereology of the lung / Jan Philipp Schneider, Matthias Ochs -- Quantitative 3D cell-based assay performed with cellular spheroids and fluorescence microscopy / Nariman Ansari, Stefanie Müller, Ernst H.K. Stelzer, Francesco Pampaloni -- Optimizing fluorescence excitation and detection for intravital two-photon microscopy / Dan Suan, Henry R. Hampton, Michio Tomura, Osami Kanagawa, Tatyana Chtanova, Tri Giang Phan -- Two-dimensional crystallization by dialysis for structural studies of membrane proteins by the cryo-EM method electron crystallography / Matthew C. Johnson, Ingeborg Schmidt-Krey -- In situ hybridization / Chung-Jui Tsai, Scott A. Harding -- RNA imaging : tracking in real-time RNA transport in neurons using molecular beacons and confocal microscopy / Angélica Zepeda, Clorinda Arias, Fabian Flores-Jasso, Luis Vaca.
  • Digital
    Yoshiharu Takayama.
    Springer2012
    "Lactoferrin was first identified as an iron-binding glycoprotein abundantly found in milk. It has been considered to play important roles in host defense, since lactoferrin shows broad spectrum of anti-bacterial and anti-viral activities, arising from its iron-chelating property. Furthermore, lactoferrin can regulate the function of innate and adaptive immune cells and exhibits immuno-modulating properties. These activities of lactoferrin implicate that potential use of lactoferrin as a wound healing agent. In addition, this book shows that lactoferrin is a potent regulator of dermal fibroblasts and epidermal keratinocytes, and promotes skin or corneal epithelial wound healing by increasing their proliferation, migration or deposition of extracellular matrix (ECM) components such as collagen and hyaluronan. This book provides the scientific background on use of lactoferrin as a wound healing agent. Indeed, lactoferrin exerts its biological effects by binding to specific lactoferrin receptors on target cells. This book also covers the in-depth information on roles of lactoferrin as a signaling molecule."--Publisher's website.
  • Digital
    edited by Shizuya Saika, Liliana Werner, Frank J. Lovicu.
    Springer2014
    This book is the first to summarize the current knowledge of the cell biology of lens epithelial cells in relation to and in the development of posterior capsular opacification (PCO). PCO remains the most common long-term complication of modern cataract surgery, occurring months or years after cataract surgery, unlike most other complications that tend to arise during or soon after the procedure. Opacification of the posterior capsule appears to be linked to lens epithelial cells that are left behind in the eye during cataract removal. These cells proliferate, migrate across the posterior lens capsule, and undergo changes that result in fibrous or pearl-type opacities in the capsule. The first section of the text explains the molecular mechanism and biology of lens epithelial cells that lead to the incidence of PCO. In the second part, in addition to a description of the mechanism and pathological condition of PCO, surgical methods and devices for preventing PCO are discussed in detail. Lens Epithelium and Capsular Opacification will benefit not only young clinical residents and junior researchers, but also established faculty in the clinical or basic academic field.
  • Digital
    edited by Robert B. Ashman.
    Springer2012
    ENU-based phenotype-driven screening / Vera M. Ripoll, Philip L. Kong, and Paul K. Potter -- Detection and quantification of cytokines and other biomarkers / Evan L. Chiswick ... [et al.] -- Flow cytometry analysis of cell cycling and proliferation in mouse hematopoietic stem and progenitor cells / Valérie Barbier ... [et al.] -- Flow cytometry measurement of bone marrow perfusion in the mouse and sorting of progenitors and stems cells according to position relative to blood flow in vivo / Valérie Barbier ... [et al.] -- Analyzing cell death events in cultured leukocytes / Karin Christenson, Fredrik B. Thorén, and Johan Bylund -- Towards a four-dimensional view of neutrophils / Ben A. Croker, Andrew W. Roberts, and Nicos A. Nicola -- Isolation of human and mouse neutrophils ex vivo and in vitro / Yan Hu -- Measurement of oxidative burst in neutrophils / Yu Chen and Wolfgang G. Junger -- Measurement of neutrophil elastase, proteinase 3, and cathepsin G activities using intramolecularly quenched fluorogenic substrates / Brice Korkmaz ... [et al.] -- The macrophage / Chris P. Verschoor, Alicja Puchta, and Dawn M.E. Bowdish -- Generation and characterization of MacGreen mice, the CFS1R-EGFP transgenic mice / R. Tedjo Sasmono and Elizabeth Williams -- Generation of mouse bone marrow-derived macrophages / Silvia Manzanero -- Isolation and differentiation of monocytes-macrophages from human blood / Dipti Vijayan -- In vitro measurement of phagocytosis and killing of Cryptococcus neoformans by macrophages / André Moraes Nicola and Arturo Casadevall -- Measuring the inflammasome / Olaf Gross -- Arginine and macrophage activation / Mònica Comalada -- Immunodetection of granzyme B tissue distribution and cellular localisation / Catherina H. Bird ... [et al.] -- Detection of human and mouse granzyme B activity in cell extracts / Sarah Elizabeth Stewart ... [et al.] -- T cell transfer model of colitis : a great tool to assess the contribution of T cells in chronic intestinal inflammation / Rajaraman Eri, Michael A. McGuckin, and Robert Wadley -- Measurement of nitrite in urine by gas chromatography-mass spectrometry / Dimitrios Tsikas ... [et al.].
  • Digital
    Luis Alberto Chia.
    The intestinal epithelium is one of the most rapidly proliferating tissues in the body. A complete turnover of the epithelium occurs every 3-5 days in the mouse, a process that is maintained by a small population of intestinal stem cells (ISCs) that reside in the crypt bases. The signals that regulate the behavior of these ISCs are still poorly understood. However, the recent identification of genes that mark functional stem cells has yielded insights into how ISCs are regulated and maintained. Here, we demonstrate that Bmi1 and Lgr5 mark two functionally distinct ISCs in vivo. Lgr5 marks mitotically active ISCs that exhibit exquisite sensitivity to canonical Wnt modulation, contribute robustly to homeostatic regeneration, and are quantitatively ablated by irradiation. In contrast, Bmi1 marks quiescent ISCs that are insensitive to Wnt perturbations, contribute weakly to homeostatic regeneration, and are resistant to high dose radiation injury. Post-irradiation, however, the normally quiescent Bmi1+ ISCs dramatically proliferate to clonally repopulate multiple contiguous crypts and villi. Clonogenic culture of isolated single Bmi1+ ISCs yields long-lived self-renewing spheroids of intestinal epithelium that produce Lgr5-expressing cells, thereby establishing a lineage relationship between these two populations in vitro. Taken together, these data provide direct evidence that Bmi1 marks quiescent, injury-inducible reserve ISCs that exhibit striking functional distinctions from Lgr5+ ISCs, and support a model whereby distinct ISC populations facilitate homeostatic versus injury-induced regeneration. How these ISCs maintain their stemness remains unclear. Paneth cells have been suggested to serve as niche cells for the Lgr5+ ISCs, perhaps through the secretion of essential paracrine factors, but recent reports clearly demonstrate that Paneth cells are not required for Lgr5+ ISC maintenance. Recently, the G-protein-coupled receptors Lgr4-6 were reported to associate with Wnt receptors to mediate R-spondin signaling. Given the importance of Wnt/R-spondin signaling in intestinal crypt maintenance, we tested the in vivo function of Lgr5 by adenovirus-mediated overexpression of the soluble ligand-binding Lgr5 extracellular domain. Circulating Lgr5 ectodomain induced the migration of Paneth cells from the crypts, their eventual loss, and a concomitant disappearance of Lgr5+ ISCs. Paneth cell migration was associated with downregulation of Wnt signaling and its target EphB3. The loss of Lgr5+ ISCs did not affect maintenance of the intestinal epithelium, nor did Lgr5+ stem cells disappear from non-intestinal organs. Together, these findings characterize an easily tractable experimental model for the in vivo deletion of Lgr5+ ISCs, and suggest that Lgr receptors function to actively maintain Lgr5+ ISCs in vivo.
  • Digital
    edited by Hélio Chiarini-Garcia, Rossana C.N. Melo.
    Springer2011
    Glycol methacrylate embedding for improved morphological, morphometrical, and immunohistochemical investigations under light microscopy : testes as a model / Hélio Chiarini-Garcia, Gleydes Gambogi Parreira, and Fernanda R.C.L. Almeida -- Histological processing of teeth and periodontal tissues for light microscopy analysis / Gerluza Aparecida Borges Silva, Adriana Moreira, and José Bento Alves -- Large plant samples: how to process for GMA embedding / Élder Antônio Sousa Paiva, Sheila Zambello de Pinho, and Denise Maria Trombert Oliveira -- Image cytometry : nuclear and chromosomal DNA quantification / Carlos Roberto Carvalho, Wellington Ronildo Clarindo, and Isabella Santiago Abreu -- Histological approaches to study tissue parasitism during the experimental Trypanosoma cruzi infection / Daniela L. Fabrino, Grazielle A. Ribeiro, Livia Teixeira, and Rossana C.N. Melo -- Intravital microscopy to study leukocyte recruitment in vivo / Vanessa Pinho ... [et al.] -- Introduction to fluorescence microscopy / Ionita C. Ghiran -- Using the fluorescent styryl dye FM1-43 to visualize synaptic vesicles exocytosis and endocytosis in motor nerve terminals / Ernani Amaral, Silvia Guatimosim, and Cristina Guatimosim -- Imaging lipid bodies within leukocytes with different light microscopy techniques / Rossana C.N. Melo ... [et al.] -- Eicosa cell : an immunofluorescent-based assay to localize newly synthesized eicosanoid lipid mediators at intracellular sites / Christianne Bandeira-Melo, Peter F. Weller, and Patricia T. Bozza -- Nestin-driven green fluorescent protein as an imaging marker for nascent blood vessels in mouse models of cancer / Robert M. Hoffman -- Imaging calcium sparks in cardiac myocytes / Silvia Guatimosim, Cristina Guatimosim, and Long-Sheng Song -- Light microscopy in aquatic ecology: methods for plankton communities studies / Maria Carolina S. Soares ... [et al.] -- Fluorescence immunohistochemistry in combination with differential interference contrast microscopy for studies of semi-ultrathin specimens of epoxy resin-embedded samples / Shin-ichi Iwasaki and Hidekazu Aoyagi.
  • Digital
    edited by Gregg G. Gundersen, Howard J. Worman.
    Springer2018
    EM tomography of meiotic LINC complexes / Marie-Christin Spindler, Frederik Helmprobst, Christian Stigloher, and Ricardo Benavente -- Recombinant purification of the periplasmic portion of the LINC complex / Victor E. Cruz and Thomas U. Schwartz -- Analysis of high molecular weight isoforms of nesprin-1 and nesprin-2 with vertical agarose gel electrophoresis / Chloe Potter and Didier Hodzic -- Analysis of nesprin-2 interaction with its binding partners and actin / Susumu Antoku and Gregg G. Gundersen -- Interactions of nesprin-4-containing LINC complexes in outer hair cells explored by BioID / Brian Burke -- Using nesprin tension sensors to measure force on the LINC complex / Paul T. Arsenovic and Daniel E. Conway -- Analyzing mechanotransduction through the LINC complex in isolated nuclei / Nejma Belaadi, Angélique Millon-Frémillon, Julien Aureille, and Christophe Guilluy -- Direct force probe for nuclear mechanics / Vincent J. Tocco, Srujana Neelam, Qiao Zhang, Richard B. Dickinson, and Tanmay P. Lele -- Centrifugal displacement of nuclei in adherent cells to study LINC complex-dependent mechanisms of homeostatic nuclear positioning / Ruijun Zhu and Gregg G. Gundersen -- Assembly and use of a microfluidic device to study cell migration in confined environments / Jeremy Keys, Aaron Windsor, and Jan Lammerding -- Investigating LINC complex protein homo-oligomerization in the nuclear envelopes of living cells using fluorescence fluctuation spectroscopy / Jared Hennen, Isaac Angert, Kwang-Ho Hur, G. W. Gant Luxton, and Joachim D. Mueller -- Functional analysis of the yeast LINC complex using fluctuation spectroscopy and super-resolution imaging / Jay R. Unruh, Brian D. Slaughter, and Sue L. Jaspersen -- Genetic analysis of nuclear migration and anchorage to study LINC complexes during development of Caenorhabditis elegans / Heidi N. Fridolfsson, Leslie A. Herrera, James N. Brandt, Natalie E. Cain, Greg J. Hermann, and Daniel A. Starr -- High-resolution imaging methods to analyze LINC complex function during Drosophila muscle eevelopment / Alexander L. Auld, Mary Ann Collins, Torrey R. Mandigo, and Eric S. Folker -- Computational methods for studying the plant nucleus / Axel Poulet, Xiao Zhou, Kentaro Tamura, Iris Meier, Christophe Tatout, Katja Graumann, and David E. Evans -- Investigation of nuclear periphery protein interactions in plants using the membrane yeast two-hybrid (MbY2H) system / Maxime Voisin, Emmanuel Vanrobays, and Christophe Tatout -- Immunolabeling protocols for studying meiosis in plant mutants defective for nuclear envelope components / Javier Varas and Mónica Pradillo -- Generation and analysis of striated muscle selective LINC complex protein mutant mice / Matthew J. Stroud, Xi Fang, Jennifer Veevers, and Ju Chen -- In vitro system to measure the positioning, stiffness, and rupture of the nucleus in skeletal muscle / William Roman, Mafalda R. Pimentel, and Edgar R. Gomes -- Functional analysis of LINC complexes in the skin / Iakowos Karakesisoglou, Carmen Mross, and Angelika A. Noegel -- Detection of SUN1 splicing variants at the mRNA and protein levels in cancer / Ayaka Matsumoto, Nariaki Matsuura, and Miki Hieda -- Next-generation sequencing and mutational analysis : implications for genes encoding LINC complex proteins / Peter L. Nagy and Howard J. Worman.
  • Digital
    edited by Hongyuan Yang and Peng Li
    ScienceDirect2013
    This new volume of Methods in Cell Biology looks at lipid droplets LDs, covering sections on analyses of LDs in model systems, cell/tissue-specific analyses of LDs and imaging and in vitro analyses of LD biogenesis and growth. Chapters are written by experts in the field. With cutting-edge material, this comprehensive collection is intended to guide researchers of LDs for years to come. Covers sections on analyses of lipid droplets (LDs) in model systems, cell/tissue-specific analyses of LDs and imaging, and in vitro analyses of LD biogenesis and growth Chapters are written by experts in t
  • Digital/Print
    Daniel G.S. Capelluto, editor.
    Digital : Springer2013
    Print2013
    Sphingomyelin and its role in cellular signaling / Mahua Chakraborty and Xian-Cheng Jiang -- The driving force of alpha-synuclein insertion and amyloid channel formation in the plasma membrane of neural cells: key role of ganglioside- and cholesterol-binding domains / Jacques Fantini and Nouara Yahi -- The enigmatic role of sulfatides: new insights into cellular functions and mechanisms of protein recognition / Shuyan Xiao, Carla V. Finkielstein, and Daniel G. S. Capelluto -- Phosphoinositides and PDZ domain scaffolds / Anna Maria Wawrzyniak, Rudra Kashyap and Pascale Zimmermann -- PtdIns(4)P signalling and recognition systems / Marc Lenoir and Michael Overduin -- PtdIns(4,5)P2-mediated cell signaling: emerging principles and PTEN as a paradigm for regulatory mechanism / Arne Gericke, Nicholas R. Leslie, Mathias Lösche, and Alonzo H. Ross -- Role of phosphatidylinositol 3,4,5-trisphosphate in cell signaling / Robert D Riehle, Sinziana Cornea and Alexei Degterev -- The role of phosphoinositides and inositol phosphates in plant cell signaling / Glenda E. Gillaspy -- Phosphatidic acid-mediated signaling / Yu Liu, Yuan Su, and Xuemin Wang -- Phosphatidylserine-mediated cellular signaling / Jason G. Kay and Sergio Grinstein -- Cardiolipin-mediated cellular signaling / Vinay A. Patil and Miriam L. Greenberg.
  • Digital
    edited by Mark Waugh.
    Springer2016
    Method for assaying the lipid kinase phosphatidylinositol-5-phosphate 4-kinase α in quantitative high-throughput screening (qHTS) bioluminescent format / Mindy I. Davis, Atsuo T. Sasaki, and Anton Simeonov -- Assaying ceramide synthase activity in vitro and in living cells using liquid chromatography-mass spectrometry / Xin Ying Lim, Russell Pickford, and Anthony S. Don -- Fluorescent assays for ceramide synthase activity / Timothy A. Couttas and Anthony S. Don -- Identification of the interactome of a palmitoylated membrane protein, phosphatidylinositol 4-kinase type II alpha / Avanti Gokhale ... [et al.] -- Measurement of long-chain fatty Acyl-CoA synthetase activity / Joachim Füllekrug and Margarete Poppelreuther -- Qualitative and quantitative in vitro analysis of phosphatidylinositol phosphatase substrate specificity / Laura Ren Huey Ip and Christina Anja Gewinner -- Luciferase reporter assays to assess liver X receptor transcriptional activity / Matthew C. Gage, Benoit Pourcet, and Inés Pineda-Torra -- Metabolically biotinylated reporters for electron microscopic imaging of cytoplasmic membrane microdomains / Kimberly J. Krager and John G. Koland -- Fluorescence recovery after photobleaching analysis of the diffusional mobility of plasma membrane proteins : HER3 mobility in breast cancer cell membranes / Mitul Sarkar and John G. Koland -- Isolation and analysis of detergent-resistant membrane fractions / Massimo Aureli ... [et al.] -- Detection of isolated mitochondria-associated ER membranes using the sigma-1 receptor / Abasha Lewis, Shang-Yi Tsai, and Tsung-Ping Su -- Using surface plasmon resonance to quantitatively assess lipid-protein interactions / Kathryn Del Vecchio and Robert V. Stahelin -- Analyzing protein-phosphoinositide interactions with liposome flotation assays / Ricarda A. Busse ... [et al.] -- High-throughput fluorometric assay for membrane-protein interaction / Wonhwa Cho, Hyunjin Kim, and Yusi Hu -- Guidelines for the use of protein domains in acidic phospholipid imaging / Matthieu Pierre Platre and Yvon Jaillais -- Analysis of sphingolipid synthesis and transport by metabolic labeling of cultured cells with [3H]serine / Neale D. Ridgway -- Determination and characterization of tetraspanin-associated phosphoinositide-4 kinases in primary and neoplastic liver cells / Krista Rombouts and Vinicio Carloni -- Analysis of the phosphoinositide composition of subcellular membrane fractions / Deborah A. Sarkes and Lucia E. Rameh -- Single-molecule imaging of signal transduction via GPI- anchored receptors / Kenichi G. N. Suzuki -- Measuring phosphatidylinositol generation on biological membranes / Mark Waugh -- Assay for CDP-diacylglycerol generation by CDS in membrane fractions / Mark Waugh.
  • Digital
    edited by Gilbert Di Paolo, Markus R. Wenk.
    ScienceDirect2012
    Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding / Min Wu, Pietro de Camilli -- Studying lipids involved in the endosomal pathway / Christin Bissig, Shem Johnson, Jean Gruenberg -- Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking / Jean-Baptiste Manneville, Cécile Leduc, Benoit Sorre, Guillaume Drin -- Reconstituting multivesicular body biogenesis with purified components / Thomas Wollert -- Approaches to the study of Atg8-mediated membrane dynamics in vitro / Anjai Jotwani, Diana N. Richerson, Isabelle Motta, Omar Julca-Zevallos, Thomas J. Melia -- Reconstitution assay system for ceramide transport with semi-intact cells / Keigo Kumagai, Masahiro Nishijima, Kentaro Hanada -- Visualizing mitochondrial lipids and fusion events in mammalian cells / Huiyan Huang, Michael A. Frohman -- Targeted and non-targeted analysis of membrane lipids using mass spectrometry / Xue Li Guan, Markus R. Wenk -- Modulation of host phosphoinositide metabolism during salmonella invasion by the type III secreted effector SopB / Bernhard Roppenser, Sergio Grinstein, John H. Brumell -- Acute manipulation of phosphoinositide levels in cells / Belle Chang-Ileto, Samual G. Frere, Gilbert Di Paolo -- Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins / Marnix Wieffer, Volker Haucke, Michael Krauß -- Phosphoinositides at the neuromuscular junction of drosophila melanogaster : a genetic approach / Jan R. Slabbaert, Thang Manh Khuong, Patrik Verstreken -- Devising powerful genetics, biochemical and structural tools in the functional analysis of phosphatidylinositol transfer proteins (PITPs) across diverse species / James M. Davison, Vytas A. Bankaitis, Ratna Ghosh -- Genome-wide screens for gene products regulating lipid droplet dynamics / Weihua Fei, Hongyuan Yang -- The three dimensionality of cell membranes : lamellar to cubic membrane transition as investigated by electron microsocopy / Ketpin Chong, Yuru Deng -- Quantitative imaging of lipid metabolism in yeast : from 4D analysis to high content screens of mutant libraries / Heimo Wolinski, Kristian Bredies, Sepp D. Kohlwein -- Analysis of cholesterol trafficking with fluorescent probes / Frederick R. Maxfield, Daniel Wüstner -- Fluorescence correlation methods for imaging cellular behavior of sphingolipid-interacting probes / Rachel Kraut, Nirmalya Bag, Thorsten Wohland -- Monitoring phospholipid dynamics during phagocytosis : application of genetically-encoded fluorescent probes / Helen Sarantis, Sergio Grinstein -- Genetically encoded probes for phosopatidic acid / Nawal Kassas, Petra Tryoen-Tóth, Matthias Corrotte, Tamou Thahouly, Marie-France Bader, Nancy J. Grant, Nicolas Vitale.
  • Digital
    edited by Gerard G.M. D'Souza.
    Springer2017
  • Digital
    edited by Takahiro Ochiya.
    Springer2012
    Purification and culture of fetal mouse hepatoblasts that are precursors of mature hepatocytes and biliary epithelial cells / Nobuyoshi Shiojiri and Miho Nitou -- Clinical uses of liver stem cells Clinical uses of liver stem cells / Yock Young Dan -- Identification and isolation of adult liver stem/progenitor cells / Minoru Tanaka and Atsushi Miyajima -- Isolation and purification method of mouse fetal hepatoblasts / Luc Gailhouste -- Isolation of hepatic progenitor cells from the galactosamine-treated rat liver / Norihisa Ichinohe, Junko Kon, and Toshihiro Mitaka -- Purification of adipose tissue mesenchymal stem cells and differentiation toward hepatic-like cells / Agnieszka Banas -- Development of immortalized hepatocyte-like cells from hMSCs / Adisak Wongkajornsilp, Khanit Sa-ngiamsuntorn, and Suradej Hongeng -- Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages / Shohei Wakao [and others] -- Generation and hepatic differentiation of human iPS cells / Tetsuya Ishikawa, Keitaro Hagiwara, and Takahiro Ochiya -- Efficient hepatic differentiation from human iPS cells by gene transfer / Kenji Kawabata, Mitsuru Inamura, and Hiroyuki Mizuguchi -- "Tet-on" system toward hepatic differentiation of human mesenchymal stem cells by hepatocyte nuclear factor / Goshi Shiota and Yoko Yoshida -- SAMe and HuR in liver physiology / Laura Gomez-Santos [and others] -- Transdifferentiation of mature hepatocytes into bile duct/ductule cells within a collagen gel matrix / Yuji Nishikawa -- Identification of cancer stem cell-related microRNAs in hepatocellular carcinoma / Junfang Ji and Xin Wei Wang -- Intravenous human mesenchymal stem cells transplantation in NOD/SCID mice preserve liver integrity of irradiation damage / Moubarak Mouiseddine [and others] -- Engineering of implantable liver tissues / Yasuyuki Sakai [and others] -- Mesenchymal stem cell therapy on murine model of nonalcoholic steatohepatitis / Yoshio Sakai and Shuichi Kaneko.
  • Digital
    Pio Zeppa, Immacolata Cozzolino.
    Karger2018
    Fine-needle cytology : technical procedures and ancillary techniques -- Lymph nodal structure and cytological patterns -- Lymphadenitis and lymphadenopathy -- Non-Hodgkin lymphoma -- Hodgkin lymphoma -- Paediatric lymphadenopathies -- Immunodeficiency-associated lymphoproliferative disorders -- Extranodal and deeply located lymphoproliferative processes -- Metastases -- Lymph node haematopoietic and histiocytic proliferatons and other lymphoid organs.
  • Digital
    edited by Karin Öllinger, Hanna Appelqvist.
    Springer2017
  • Digital
    edited by Frances Platt, Nick Platt.
    ScienceDirect2015
  • Digital
    David S. Goodsell.
    Springer2009
    Introduction -- Molecular machines -- The processes of living -- Molecules in cells: Escherichia coli -- A human cell: The advantages of compartments -- The human body: The advantages of specialization -- Life and death -- Viruses -- You and your molecules.
  • Digital
    edited by Germain Rousselet.
    Springer2018
    Culture of human monocyte-derived macrophages / Aoife Kelly, Aleksander M. Grabiec, and Mark A. Travis -- Simple multistep protocol for differentiating human induced pluripotent stem cells into functional macrophages / Chandrayana Mukherjee, Christine Hale, and Subhankar Mukhopadhyay -- Growing murine bone marrow-derived macrophages / Anaïs Assouvie, Lisa P. Daley-Bauer, and Germain Rousselet -- Immortalization of murine bone marrow-derived macrophages / Dominic De Nardo, Dhan V. Kalvakolanu, and Eicke Latz -- Isolation and identification of murine serous cavity macrophages / Calum C. Bain and Stephen J. Jenkins -- Isolation and identification of interstitial macrophages from the lungs using different digestion enzymes and staining strategies / Shaikh M. Atif, Sophie L. Gibbings, and Claudia V. Jakubzick -- Isolation and phenotyping of adult mouse microglial cells / Kathleen Grabert and Barry W. McColl -- Isolation and phenotyping of bone marrow macrophages / Marion Chalot -- Isolation and phenotyping of intestinal macrophages / Vanessa Petit -- Phenotyping tumor-associated macrophages / R. Ian Cumming and Yen-Rei A. Yu -- Activating murine macrophages In Vitro / Elizabeth Dalby -- Polarizing macrophages in vitro / Xuan Huang, Yong Li, Mingui Fu, and Hong-Bo Xin -- Viral replication assay in bone marrow-derived macrophages / Linda Roback and Lisa P. Daley-Bauer -- Macrophage bactericidal assays / Mourad Aribi -- Quantitative phagocytosis assays in primary and cultured macrophages / Fernando Montaño, Sergio Grinstein, and Roni Levin -- Observing frustrated phagocytosis and phagosome formation and closure using total internal reflection fluorescence microscopy (TIRFM) / Anna Mularski, Florence Marie-Anaïs, Julie Mazzolini, and Florence Niedergang -- Chromatin immunoprecipitation in macrophages / Germain Rousselet -- Transfecting macrophages / Andrea-Anneliese Keller, Marten B. Maess, Michael Schnoor, Berith Scheiding, and Stefan Lorkowski -- In vitro migration assays / Lewis Taylor, Carlota Recio, David R. Greaves, and Asif J. Iqbal -- Cytokine-induced acute inflammatory monoarticular arthritis / Adrian Achuthan, Ming-Chin Lee, Reem Saleh, Andrew J. Fleetwood, John A. Hamilton, and Andrew D. Cook -- Characterizing activation, proliferation, and ontogeny of murine macrophages in parasitic helminth infections / Dominik Rückerl -- Genetic models of macrophage depletion / Li Hua, Jiayuan Shi, Leonard D. Shultz, and Guangwen Ren -- Depleting macrophages in vivo with clodronate-liposomes / Stéphanie G. Moreno -- Cre driver mice targeting macrophages / Jiayuan Shi, Li Hua, Danielle Harmer, Peishan Li, and Guangwen Ren.
  • Print
    Malgorzata Kloc, editor.
    Evolutionary Aspects of Macrophages Polarization / Eva-Stina Edholm, Kun Hyoe Rhoo, and Jacques Robert -- Development and Functional Differentiation of Tissue-Resident Versus Monocyte-Derived Macrophages in Inflammatory Reactions Paola Italiani and Diana Boraschi -- Hofbauer Cells: Placental Macrophages of Fetal Origin / Leticia Reyes, Bryce Wolfe, and Thaddeus Golos -- Mesenchymal Stem Cells Direct the Immunological Fate of Macrophages / Patricia Luz-Crawford, Christian Jorgensen, and Farida Djouad -- Monocyte/Macrophage: NK Cell Cooperation--Old Tools for New Functions / Elżbieta Wałajtys-Rode and Jolanta M. Dzik -- Macrophages in Invertebrates: From Insects and Crustaceans to Marine Bivalves / Prasad Abnave, Xavier Muracciole, and Eric Ghigo -- F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen / Alexandra dos Anjos Cassado -- Immunobiology of Nitric Oxide and Regulation of Inducible Nitric Oxide Synthase / Martin Lee [and others] -- Role for Mechanotransduction in Macrophage and Dendritic Cell Immunobiology / Svenja F. B. Mennens, Koen van den Dries, and Alessandra Cambi -- Macrophages' Role in Tissue Disease and Regeneration / Lewis Gaffney [and others] -- Macrophages and Their Contribution to the Development of Atherosclerosis / Yuri V. Bobryshev [and others] -- Macrophage Dysfunction in Respiratory Disease / Kylie B. R. Belchamber and Louise E. Donnelly -- Activation of Macrophages in Response to Biomaterials / Jamie E. Rayahin and Richard A. Gemeinhart -- Macrophage Differentiation in Normal and Accelerated Wound Healing / Girish J. Kotwal and Sufan Chien -- Macrophages and RhoA Pathway in Transplanted Organs / Yianzhu Liu [and others].
  • Digital
    edited by Neil E. Reiner.
    Springer2009
    Generation of retroviral macrophage cDNA expression libraries and functional screening for surface receptors / Georgia Schäfer and Gordon D. Brown -- Generation of murine growth factor-dependent long-term dendritic cell lines to investigate host-parasite interactions / Alessandra Mortellaro ... [et al.] -- Macrophage activation : classical vs. alternative / Andrea Classen, Jorge Lloberas, and Antonio Celada -- Analysis of macrophage phagocytosis : quantitative assays of phagosome formation and maturation using high-throughput florescence microscopy / Benjamin E. Steinberg and Sergio Grinstein -- Detection of activated Rab7 GTPase with an immobilized RILP probe / Jim Sun ... [et al.] -- Probing in vivo origins of mononuclear phagocytes by conditional ablation and reconstitution / Chen Varol, Limor Landsman, and Steffen Jung -- Multivalent protein probes for the identification and characterization of cognate cellular ligands for myeloid cell surface receptors / Hsi-Hsien Lin ... [et al.] -- Exploiting Fc chimaeric proteins for the identification of ligands specific for the mannose receptor / Luisa Martinez-Pomares -- The expression of exogenous genes in macrophages : obstacles and opportunities / Xia Zhang, Justin P. Edwards, and David M. Mosser -- Genetic modification of murine dendritic cells by RNA transfection / Maurizio Ceppi, Enrico Schmidt, and Philippe Pierre -- Recording phagosome maturation through the real-time, spectrofluorometric measurement of hydrolytic activities / Robin M. Yates, Albin Hermetter, and David G. Russell -- Derivation and characterization of murine alternatively activated (M2) macrophages / Victor W.H. Ho and Laura M. Sly -- Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology / Mattias Svensson -- Generation, culture, and flow-cytometric characterization of primary mouse macrophages / Ulrike Schleicher and Christian Bogdan -- Analyzing classical and alternative macrophage activation in macrophage/neutrophil-specific IL-4 receptor-alpha-deficient mice / Frank Brombacher ... [et al.] -- Analysis of matrix metalloproteinase secretion by macrophages / Paul T. Elkington, Justin A. Green, and Jon S. Friedland -- Identification of pathogen receptors on dendritic cells to understand their function and to identify new drug targets / Lot de Witte ... [et al.] -- Stable lentiviral vector-mediated gene silencing in human monocytic cell lines / Jimmy S. Lee and Neil E. Reiner -- Use of fluorescent probes to detect lipid signaling intermediates in macrophages / Vivan Kwok, Eric Vachon, and Gregory P. Downey -- Large-scale phagosome preparation / Adrien F. Vinet and Albert Descoteaux -- Coordinate regulation of sphingosine kinase and actin dynamics / Shankar S. Iyer and David J. Kusner.
  • Digital/Print
    edited by M. Zborowski, J.J. Chalmers.
    Digital : ScienceDirect2008
    Print2008
  • Digital
    Kyle E. Orwig, Brian P. Hermann, editors.
    Springer2011
    Scientists investigating germ cells have, over the past 15 years, originated discoveries and innovations that give us valuable insights into the mechanisms that regulate not just stem cell function, but human development in its widest sense. With contributions from some of the leading researchers in the field, Male Germline Stem Cells: Developmental and Regenerative Potential assesses the implications of these discoveries for understanding the fundamental biology of germline stem cells as well as their potential for human stem cell-based therapies. This monograph covers many of the fundamental issues now being explored by today's generation of stem cell researchers, including the field's potential for regenerative medicine.
  • Digital
    edited by Hayden A. Homer.
    Springer2013
    Studying recombination in mouse oocytes / Xianfei Sun and Paula E. Cohen -- Analysis of recombinants in female mouse meiosis / Esther de Boer, Maria Jasin, and Scott Keeney -- Studying meiosis-specific cohesins in mouse embryonic oocytes / Ji-hye Kim [and others] -- Immunohistochemical approaches to the study of human fetal ovarian development / Jing He [and others] -- Protein kinase assays for measuring MPF and MAPK activities in mouse and rat oocytes and early embryos / Jacek Z. Kubiak -- Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis I in mouse oocytes / Janet E. Holt, Simon I.R. Lane, and Keith T. Jones -- Using FRET to study ranGTP gradients in live mouse oocytes / Julien Dumont and Marie-Hélène Verlhac -- Making cRNA for microinjection and expression of fluorescently tagged proteins for live-cell imaging in oocytes / Mark Levasseur -- RNAi-based methods for gene silencing in mouse oocytes / Paula Stein, Petr Svoboda, and Richard M. Schultz -- Micro-injection of morpholino oligonucleotides for depleting securin in mouse oocytes / Petros Marangos -- Measuring transport and accumulation of radiolabeled substrates in oocytes and embryos / Jay M. Baltz, Hannah E. Corbett, and Samantha Richard -- Immunofluorescence staining of spindles, chromosomes, and kinetochores in human oocytes / Solon Riris [and others] -- Studying the roles of aurora-C kinase during meiosis in mouse oocytes / Kuo-Tai Yang [and others] -- Chromosome spreads with centromere staining in mouse oocytes / Jean-Philippe Chambon, Khaled Hached, and Katja Wassmann -- Preparation of mammalian oocytes for transmission electron microscopy / Lynne Anguish and Scott Coonrod -- Measuring Ca² oscillations in mammalian eggs / Karl Swann -- Counting chromosomes in intact eggs / Teresa Chiang and Michael A. Lampson -- Free-hand bisection of mouse oocytes and embryos / Zbigniew Polanski and Jacek Z. Kubiak -- Microarray-CGH for the assessment of aneuploidy in human polar bodies and oocytes / Souraya Jaroudi and Dagan Wells -- Nuclear transfer in the mouse oocyte / Eiji Mizutani, Atsuo Ogura, and Teruhiko Wakayama.
  • Digital
    edited by Finian Martin, Torsten Stein, Jillian Howlin.
    Springer2017
  • Digital
    Joseph H. Antin, Harvard Medical School and Dana-Farber Brigham and Women's Cancer Center, Boston, MA, Deborah Yolin Raley, Dana-Farber Brigham and Women's Cancer Center, Boston, MA.
    Cambridge2013
    1. Rationale for transplantation -- 2. Types of transplantation -- 3. Human leukocyte antigen matching in allogeneic transplantation -- 4. Stem cell source -- 5. Pre-transplant evaluation and counseling of patient and donor -- 6. Conditioning regimens -- 7. Stem cell infusion -- 8. ABO compatibility -- 9. Engraftment -- 10. Preventative care -- 11. Transplant-related complications -- 12. Overview of acute and chronic graft-versus-host disease -- 13. Acute graft-versus-host disease and staging -- 14. Graft-versus-host prophylactic regimens -- 15. Treatment guidelines for acute graft-versus-host disease -- 16. Chronic graft-versus-host disease -- 17. Engraftment syndrome -- 18. Infectious disease -- 19. Graft rejection and failure -- 20. Gastrointestinal complications -- 21. Oral health in stem cell transplantation -- 22. Pulmonary complications -- 23. Veno-occlusive disease -- 24. Special transfusion-related situations -- 25. Cardiovascular complications -- 26. Neurologic complications -- 27. Cystitis -- 28. Donor lymphocyte infusion -- 29. Transplantation: regulation and accreditation -- Index.
  • Digital/Print
    edited by Alasdair M. Gilfillan [and] Dean D. Metcalfe.
    Digital : Springer2011
    Print2011
    Mast cell biology : introduction and overview / Alasdair M. Gilfillan, Sarah J. Austin, and Dean D. Metcalfe -- Mast cell progenitor trafficking and maturation / Jenny Hallgren and Michael F. Gurish -- Monomeric IgE and mast cell development, survival, and function / Jun-ichi Kashiwakura, Iris M. Otani, and Toshiaki Kawakami -- Mast cell apoptosis and survival / Maria Ekoff and Gunnar Nilsson -- Regulators of Ca2+ signaling in mast cells: Potential targets for treatment of mast cell-related diseases / Hong-Tao Ma and Michael A. Beaven -- Spatio-temporal signaling in mast cells / Bridget S. Wilson, Janet M. Oliver, and Diane S. Lidke -- The mechanisms of exocytosis in mast cells / Ulrich Blank -- An emerging role for the lipid mediator sphingosine-1-phosphate in mast cell effector function and allergic disease / Ana Olivera and Juan Rivera -- Down-regulation of mast cell responses through itim containing inhibitory receptors / Laila Karra and Francesca Levi-Schaffer -- The mast cell in innate and adaptive immunity / Christopher P. Shelburne and Soman N. Abraham -- Mast cells and immunoregulation/immunomodulation / Mindy Tsai, Michele Grimbaldeston, and Stephen J. Galli -- Mast cell proteases as protective and inflammatory mediators / George H. Caughey -- Mast cells in lung inflammation / Elena P. Moiseeva and Peter Bradding -- Mast cells, angiogenesis, and cancer / Domenico Ribatti and Enrico Crivellato.
  • Digital
    edited by Michael R. Hughes and Kelly M. McNagny.
    Springer2015
    Paul Ehrlich's mastzellen : a historical perspective of relevant developments in mast cell biology / Jack Ghably [and others] -- Phylogenetic profile of mast cells / Enrico Crivellato, Luciana Travan, and Domenico Ribatti -- Mast cell development and function in the Zebrafish / Sahar I Da'as, Tugce B. Balci, and Jason N. Berman -- Human mast cell and basophil/eosinophil progenitors / Gail M. Gauvreau and Judah A. Denburg -- Methods for the study of mast cell recruitment and accumulation in different tissues / Tatiana G. Jones and Michael F. Gurish -- Notch2 signaling in mast cell development and distribution in the intestine / Mamiko Sakata-Yanagimoto and Shigeru Chiba -- Mast cells in human health and disease / Erin J. DeBruin ... [e tal.] -- Emerging prominence of the cardiac mast cell as a potent mediator of adverse myocardial remodeling / Joseph S. Janicki, Gregory L. Brower, and Scott P. Levick -- Parasympathetic nervous system as a regulator of mast cell function / Paul Forsythe -- Growth of human mast cells from bone marrow and peripheral blood-derived CD34+ pluripotent hematopoietic cells / Geethani Bandara, Dean D. Metcalfe, and Arnold S. Kirshenbaum -- Isolation and characterization of human intestinal mast cells / Axel Lorentz, Gernot Sellge, and Stephan C. Bischoff -- Human mast cell activation with viruses and pathogen products / Ian D. Haidl and Jean S. Marshall -- Basic techniques to study Fc[epsilon]RI signaling in mast cells / Yuko Kawakami and Toshiaki Kawakami -- Membrane-cytoskeleton dynamics in the course of mast cell activation / Pavel Dráber and Petr Dráber -- Fc[epsilon]RI expression and dynamics on mast cells / Eon J. Rios and Janet Kalesnikoff -- Regulation of mast cell survival and apoptosis / Christine Möller Westerberg, Maria Ekoff, and Gunnar Nilsson -- Protein tyrosine phosphatases in mast cell signaling / Alexander Geldman and Catherine J. Pallen -- MicroRNA function in mast cell biology : protocols to characterize and modulate MicroRNA expression / Steven Maltby [and others] -- Assay of mast cell mediators / Madeleine Rådinger [and others] -- Induction of mast cell apoptosis by a novel secretory granule-mediated pathway / Fabio R. Melo, Sara Wernersson, and Gunnar Pejler -- Measurement of nitric oxide in mast cells with the fluorescent indicator DAF-FM diacetate / Chris D. St. Laurent, Tae Chul Moon, and A. Dean Befus -- Real-time imaging of Ca2+ mobilization and degranulation in mast cells / Roy Cohen, David A. Holowka, and Barbara A. Baird -- Flow cytometry-based monitoring of mast cell activation / Glenn Cruse, Alasdair M. Gilfillan, and Daniel Smrz -- Measurement of mast cell surface molecules by high- throughput immunophenotyping using transcription (HIT) / D. James Haddon [and others] -- Cre/loxP-based mouse models of mast cell deficiency and mast cell-specific gene inactivation / Katrin Peschke [and others] -- Evaluation of synovial mast cell functions in autoimmune arthritis / Peter A. Nigrovic and Kichul Shin -- Methods for the study of mast cells in cancer / Nichole R. Blatner, FuNien Tsai, and Khashayarsha Khazaie -- Studying mast cells in peripheral tolerance by using a skin transplantation model / Victor C. de Vries [and others] -- Function of mast cells in autoimmune glomerulonephritis / Renato C. Monteiro [and others] -- Mouse model of atopic dermatitis / Yuko Kawakami and Toshiaki Kawakami -- Mouse models of allergic asthma / Matthew Gold, David Marsolais, and Marie-Renee Blanchet -- Methods in assessment of airway reactivity in mice / Matthew Gold and Marie-Renee Blanchet.
  • Digital
    Cem Akin, editor.
    Springer2020
    This unique book offers an in-depth, best-practices guide to diagnosis and management of mastocytosis, a too-often underdiagnosed disease. Mastocytosis: A Comprehensive Guide will open with a general overview and discussion of mast cell biology, addressing tryptase and other diagnostic markers in detail. Comprehensive diagnostic criteria and classification will follow, with special emphasis on commonly-seen related manifestations: skin disease, pediatric mastocytosis, gastrointestinal indicators, osteoporosis, anaphylaxis, venom and drug allergy, and pregnancy. Mastocytosis will be an ideal resource for not only the allergist confronted with this condition, but for a growing, multi-disciplinary audience of hematologists, gastroenterologists, dermatologists, pediatricians, primary care providers and other clinicians who encounter this disease in their patients. .
  • Digital
    Carmen Molina-París, Grant Lythe, editors.
    Springer2011
    Preface -- Thymocyte development -- A review of mathematical models for T cell receptor triggering and antigen discrimination -- Dynamic tuning of T cell receptor specificity by co-receptors and costimulation -- T cell activation and function: role of signal strength -- The cyton model for lymphocyte proliferation and differentiation -- Modeling itravital two-photon data of lymphocyte migration and interaction -- Modeling lymphocyte dynamics in vivo -- Continuous-time birth and death processes: diversity maintenance of naïve T cells in the periphery -- Multivariate competition processes: a model for two competing T cell clonotypes -- Stochastic modeling of T Cell homeostasis for two competing clonotypes via the master equation -- Dendritic cell migration in the intestinal tract -- Reassessing germinal center reaction concepts -- B cell strategies of Ag recognition in a stratified immune system -- Dynamics of Peripheral regulatory and effector T cells competing for antigen presenting cells -- Mathematical models of the role of IL-2 in the interactions between helper and regulstory CD4+ T cells -- A Physicist{u2019}s approach to immunology -- Timescales of the adaptive immune response -- Using mathematical models to explore the role of cytoxic T lymphocytes in HIV infection -- Viral immunity and persistence -- Index.
  • Digital
    Margaret Gardel and Patrick Oakes.
    Atypon2015
    Cells are inherently physical entities that both experience mechanical forces from their external environment and generate their own internal forces to drive cell motion. Our particular aim here is to present the reader with an introduction to the primary tools used to measure these mechanical interactions and the material properties of cells that result from them. These approaches can be applied to a diverse array of physiological processes and systems, providing important insight into the regulatory roles of mechanical interactions in cells. We cover techniques at both the molecular and cellular scales, including those that actively and passively probe the system. Along the way we cover the fundamental principles of each approach, while emphasizing the relevant length and timescales, along with the typical magnitudes of the measurements that can be made. Each section ends by highlighting uses of the various techniques in recent relevant publications, illustrating the exciting future of cell mechanics in quantitative cell biology research.
  • Digital
    Chelsey Savannah Simmons.
    The heart is a complex integrated system that leverages mechanoelectrical signals to synchronize cardiomyocyte contraction and push blood throughout the body. Due to the heart's limited regenerative capacity and the wide variety of cardiovascular pathologies, heart disease is often studied in vitro. However, it is difficult to accurately replicate the cardiac environment outside of the body. In this dissertation, I describe an integrated strain array for cell culture that mimics the mechanical movement of the heart and enables high-throughput mechanotransduction studies. Along with mechanical strain, substrate stiffness is an important mechanical stimulus. The heart and vasculature, along with other organs, remodel in both development and disease, changing their mechanical properties. I successfully implement a method that can simultaneously tune both substrate stiffness and mechanical strain in normal and pathological ranges. Polyacrylamide gels, attached to stretchable silicone platforms by interpenetrating networks, can be stretched up to 50% without delaminating. To fully harness the potential of studying heart disease in vitro, better techniques for studying heart cell contractions are required in addition to biomimetic dynamic culture. In this dissertation, I also describe two successful new approaches to quantifying cardiomyocyte contractility using simple phase contrast videos: one for single cells plated on soft gels and one for cell monolayers attached to glass or plastic. Together, these innovations provide a suite of tools to stimulate and assess cardiovascular cells and advance our collective knowledge of cardiovascular health and disease.
  • Digital
    Andre Kamkin, Ilya Lozinsky, editors ; foreword by Maik Gollasch.
    Springer2009
    The volume dwells on the major issues of mechanical stress influencing the ion channels and intracellular signaling pathways. This book is a unique collection of reviews outlining current knowledge and future developments in this rapidly growing field. In our opinion the book presents not only the latest achievements in the field but also brings the problem closer to the experts in related medical and biological sciences as well as practicing doctors. Knowledge of the mechanisms which underlie these processes is necessary for understanding of the normal functioning of different living organs and tissues and allows to predict changes, which arise due to alterations of their environment, and possibly will allow to develop new methods of artificial intervention. We also hope that presenting the problem will attract more attention to it both from researchers and practitioners and will assist to efficiently introduce it into the practical medicine.
  • Digital
    Dmitry O. Zharkov, editor.
    Springer2020
  • Digital
    Springerv. 1-, 2008-
  • Digital
    editors, Haruhisa Inoue and Yukio Nakamu.
    Springer2019
  • Digital
    [edited by] Walter F. Boron, Emile L. Boulpaep.
    ClinicalKey2017
  • Print
    [edited by] Walter F. Boron, MD, PhD, professor, David N. and Inez Myers/Antonio Scarpa Chairman, Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio, Emile L. Boulpaep, MD, professor, Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, Connecticut.
    Status: Not Checked Out
    Medical Physiology, in its updated 2nd edition, firmly relates molecular and cellular biology to the study of human physiology and disease. Drs. Walter Boron and Emile Boulpaep and a team of leading physiologists present practical, accurate coverage emphasizing the clinical implications of the material. Each chapter explains the principles and organization of each body system, while more than 1400 high-quality, full-color line drawings and prominently featured clinical examples clarify every concept. This exceptionally detailed and comprehensive guide to physiology is ideal for a rich, straightforward, state-of-the-art understanding of this essential subject.
  • Digital
    edited by Doron Rapaport, Johannes M. Herrmann.
    Springer2013
    Quantitative analysis of cellular lipids by nano- electrospray ionization mass spectrometry / Cagakan Özbalci, Timo Sachsenheimer, and Britta Brügger -- Thin-layer chromatography of phospholipids / Rania M. Deranieh, Amit S. Joshi, and Miriam L. Greenberg -- Analysis of membrane lipid biogenesis pathways using yeast genetics / Martina Gsell and Günther Daum -- Using 2D crystals to analyze the structure of membrane proteins / Ian Collinson, Janet Vonck, and Dilem Hizlan -- Crystallization of membrane proteins / Florian G. Müller and C. Roy D. Lancaster -- Molecular dynamics simulations of membrane proteins / Kristyna Pluhackova, Tsjerk A. Wassenaar, and Rainer A. Böckmann -- Site-specific fluorescent probe labeling of mitochondrial membrane proteins / Christine T. Schwall and Nathan N. Alder -- Topology determination of untagged membrane proteins / Iris Nasie, Sonia Steiner-Mordoch, and Shimon Schuldiner -- self-assembling GFP : a versatile tool for plant (membrane) protein analyses / Katharina Wiesemann ... [et al.] -- Use of cardiolipin-containing liposomes as a model system to study the interaction between proteins and the inner mitochondrial membrane / Milit Marom and Abdussalam Azem -- Analysis of the interaction between membrane proteins and soluble binding partners by surface plasmon resonance / Zht Cheng Wu... [et al.] -- Peptide interaction with and insertion into membranes / Ron Saar-Dover, Avraham Ashkenazi, and Yechiel Shai -- Scanning fluorescence correlation spectroscopy in model membrane systems / Joseph D. Unsay and Ana J. García-Sáez -- Analyses of protein-protein Interactions by in vivo photocrosslinking in budding yeast / Takuya Shiota, Shuh-ichi Nishikawa, and Toshiya Endo -- Sedimentation velocity analytical ultracentrifugation in hydrogenated and deuterated solvents for the characterization of membrane proteins / Aline Le Roy ... [et al.] -- Membrane partitioning and translocation studied by isothermal titration calorimetry / Carolyn Vargas, Johannes Klingler, and Sandro Keller -- Analyzing membrane dynamics with live cell fluorescence microscopy with a focus on yeast mitochondria / Dirk Scholz ... [et al.] -- Analysis of protein translocation into the endoplasmic reticulum of human cells / Johanna Dudek ... [et al.] -- Assay to monitor the membrane integration of single-span proteins / Katrin Krumpe and Doron Rapaport -- Methods to study the biogenesis of membrane proteins in yeast mitochondria / Daniel Weckbecker and Johannes M. Herrmann -- Reconstitution of mitochondrial presequence translocase into proteoliposomes / Martin van der Laan, Ralf M. Zerbes, and Chris van der Does -- Single channel analysis of membrane proteins in artificial bilayer membranes / Philipp Bartsch, Anke Harsman, and Richard Wagner -- Quantification of protein complexes by blue native electrophoresis / Juliana Heidler ... [et al.] -- Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions / Anna Hjelm ... [et al.] .
  • Digital
    Joachim Krebs, editor.
    Springer2017
    "This book describes the newest discoveries on calcium signaling happening at the cellular and intracellular membranes, often exerted in so called microdomains. Calcium entry and release, its interaction with proteins and resulting events on proteins and organelles are comprehensively depicted by leading experts in the field. Knowledge about details of these highly dynamic processes rapidly increased in recent years, the book therefore provides a timely summary on the processes of calcium signaling and related membrane dynamics; it is aimed at students and researchers in biochemistry and cell biology."-- Provided by publisher.
  • Digital
    edited by Bor Luen Tang.
    Springer2015
    This second edition explores membrane trafficking research that has been at the frontier of cell and molecular biology. In the past 20 years, this field has witnessed a remarkable convergence and synergism from information gained using genetic approaches in yeast cells, and biochemical and molecular approaches in mammalian cells. This volume contains updated chapters and the inclusion of a large number of new chapters, and is divided into three parts. Part I includes biochemical and molecular genetics approaches and methods used in analysing membrane traffic, in both yeast and mammalian cell models. Part II focuses on imaging and microscopy approaches and techniques, while Part III highlights the Omics-type approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and resourceful, Membrane Trafficking, Second Edition, is a valuable resource for both experienced researchers, graduate students, and junior scientists in the field of membrane trafficking.
  • Digital
    edited by Yaoliang Tang, Buddhadeb Dawn.
    ScienceDirect2015
    Insights into the Mechanism of Exosome Formation and Secretion / Kobina Essandoh, Guo-Chang Fan -- An Overview of the Proteomic and miRNA Cargo in MSC-Derived Exosomes / Soon Sim Tan, Tian Sheng Chen, Kok Hian Tan, Sai Kiang Lim -- Exosome Function in miRNA-Mediated Paracrine Effects / Sathyamoorthy Balasubramanian, Sheeja Rajasingh, Jayakumar Thangavel, Buddhadeb Dawn, Johnson Rajasingh -- Current Methods to Purify and Characterize Exosomes / W. Michael Dismuke, Yutao Liu -- Stem Cell Extracellular Vesicles: A Novel Cell-Based Therapy for Cardiovascular Diseases / Ewa K. Zuba-Surma, Marta Adamiak, Buddhadeb Dawn -- Therapeutic Potential of Stem Cell-Derived Extracellular Vesicles in Cardioprotection and Myocardium Repair / Bin Yu, Muhammad Ashraf, Meifeng Xu -- Engineered/Hypoxia-Preconditioned MSC-Derived Exosome: Its Potential Therapeutic Applications / Wei Zhu, Han Chen, Jian'an Wang -- Exosome-Based Translational Nanomedicine: The Therapeutic Potential for Drug Delivery / Lei Lv, Qingtan Zeng, Shenjun Wu, Hui Xie, Jiaquan Chen, Xiang Jiang Guo, Changning Hao, Xue Zhang, Meng Ye, Lan Zhang -- Effect of Exosomes from Mesenchymal Stem Cells on Angiogenesis / Susmita Sahoo, Feng Dong, Lola DiVincenzo, William Chilian, Liya Yin -- Exosomes for Bone Diseases / Paulomi Sanghavi, Porter Young, Sunil Upadhyay, Mark W. Hamrick -- Diagnostic and Prognostic Applications of MicroRNA-Abundant Circulating Exosomes / Baron Arnone, Xiaoqi Zhao, Zhipeng Zou, Gangjian Qin, Min Cheng.
  • Digital
    Lucas G. Chase, Mohan C. Vemuri, editors.
    Springer2013
    Mesenchymal Stem Cells or Multipotent Mesenchymal Stromal Cells (collectively referred to as MSCs) are currently being tested in a number of clinical trials for various diseases including graft-versus-host disease, Crohn's disease, myocardial infarction, stroke, diabetes, cartilage defects, and many others. The increasing number of clinical trials globally indicates the likely therapeutic potential of this specialized adult stem cell. Over the last decade, there has been tremendous progress towards understanding an MSC's phenotype, expansion features in culture, differentiation ability and global gene and protein expression profiles. Despite the enormous progress and data accrual at the molecular and cellular levels, several questions remain as to the utility of MSCs for clinical applications. Mesenchymal Stem Cell Therapy is a comprehensive collection of information comprised of both basic and pre-clinical biological studies as well as current advances in clinical applications within the MSC therapy field. This text is a resource for both the basic life science and cell therapy researchers and includes a spectrum of review chapters from top experts in the field discussing clinical scale culture, regulatory issues, genetic engineering, disease specific applications and others.
  • Digital
    edited by Darwin J. Prockop, Donald G. Phinney, Bruce A. Bunnell.
    Springer2008
    Part I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.]. Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger. A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C. W. Zannettino. Adipose-derived stem cells / John K. Fraser ... [et al.]. Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois ... [et al.] -- Part II. Manipulation of human multipotential stromal cells. Colony forming unit assays for MSCs / Radhika Pochampally. Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe. Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe. Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi. Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prockop. Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- Part III. Isolation and characterization of murine multipotential stromal cells from bone marrow. Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney.
  • Digital
    edited by Marco Fondi.
    Springer2018
    Reconstructing high-quality large-scale metabolic models with merlin / Oscar Dias, Miguel Rocha, Eugenio Campos Ferreira, and Isabel Rocha -- Analyzing and designing cell factories with OptFlux / Paulo Vilaca, Paulo Maia, Hugo Giesteira, Isabel Rocha, and Miguel Rocha -- MONGOOSE rational arithmetic toolbox / Christopher Le and Leonid Chindelevitch -- FASTCORE family : for the fast reconstruction of compact context-specific metabolic networks models / Maria Pires Pacheco and Thomas Sauter -- Reconstruction and analysis of central metabolism in microbes / Janaka N. Edirisinghe, Jose P. Faria, Nomi L. Harris, Benjamin H. Allen, and Christopher S. Henry -- Using PSAMM for the curation and analysis of genome-scale metabolic models / Keith Dufault-Thompson, Jon Lund Steffensen, and Ying Zhang -- Integration of comparative genomics with genome-scale metabolic modeling to investigate strain-specific phenotypical differences / Jonathan Monk and Emanuele Bosi -- Template-assisted metabolic reconstruction and assembly of hybrid bacterial models / Tiziano Vignolini, Alessio Mengoni, and Marco Fondi -- Integrated host-pathogen metabolic reconstructions / Anu Raghunathan and Neema Jamshidi -- Metabolic model reconstruction and analysis of an artificial microbial ecosystem / Chao Ye, Nan Xu, Xiulai Chen, and Liming Liu -- RNA sequencing and analysis in microorganisms for metabolic network reconstruction / Eva Pinatel and Clelia Peano -- Differential proteomics based on 2D-difference in-gel electrophoresis and tandem mass spectrometry for the elucidation of biological processes in antibiotic-producer bacterial strains / Giuseppe Gallo and Andrea Scaloni -- Techniques for large-scale bacterial genome manipulation and characterization of the mutants with respect to in silico metabolic reconstructions / George C. diCenzo and Turlough M. Finan -- Computational prediction of synthetic lethals in genome-scale metabolic models using fast-SL / Karthik Raman, Aditya Pratapa, Omkar Mohite, and Shankar Balachandran -- Coupling fluxes, enzymes, and regulation in genome-scale metabolic models / Paul A. Jensen -- Dynamic flux balance analysis using DFBAlab / Jose Alberto Gomez and Paul I. Barton -- Designing optimized production hosts by metabolic modeling / Christian Jungreuthmayer, Matthias P. Gerstl, David A. Pena Navarro, Michael Hanscho, David E. Ruckerbauer, and J€urgen Zanghellini -- Optimization of multi-omic genome-scale models : methodologies, hands-on tutorial, and perspectives / Supreeta Vijayakumar, Max Conway, Pietro Li o, and Claudio Angione.
  • Digital
    Piotr Dziegiel, Bartosz Pula, Christopher Kobierzycki, Mariusz Stasiolek, Marzenna Podhorska-Okolow.
    Springer2016
    Introduction -- Metallothioneins : structure and functions -- Metallothionein-3 -- The role of metallothioneins in carcinogenesis -- Metallothioneins and immune function.
  • Digital
    edited by Miriam Dwek, Susan A. Brooks, Udo Schumacher.
    Springerv.1 (2012)
    Springerv.2 (2014)
  • Digital
    edited by Franck Perez and David J. Stephens.
    ScienceDirect2013
  • Digital
    edited by Renata Basto and Wallace F. Marshall.
    ScienceDirect2015
    Imaging centrosomes and cilia in the mouse kidney / Masato Hoshi, Jinzhi Wang, Sanjay Jain, Moe R. Mahjoub -- Ependymal cell differentiation, from monociliated to multiciliated cells / Nathalie Delgehyr, Alice Meunier, Marion Faucourt, Montserrat Bosch Grau, Laetitia Strehl, Carsten Janke, Nathalie Spassky -- Observing planar cell polarity in multiciliated mouse airway epithelial cells / Eszter K. Vladar, Yin Loon Lee, Tim Stearns, Jeffrey D. Axelrod -- Imaging and analyzing primary cilia in cardiac cells / Nicolas Diguet, Jean-Francois Le Garrec, Tommaso Lucchesi, Sigolene M. Meilhac -- Cilia in photoreceptors / Linjing Li, Manisha Anand, Kollu Nageswara Rao, Hemant Khanna -- Analysis of primary cilia in the developing mouse brain / Judith T.M.L. Paridaen, Wieland B. Huttner, Michaela Wilsch-Brauninger -- In vivo investigation of cilia structure and function using Xenopus / Eric R. Brooks, John B. Wallingford -- A quantitative approach to study endothelial cilia bending stiffness during blood flow mechanodetection in-vivo / Francesco Boselli, Jacky G. Goetz, Gilles Charvin, Julien Vermot -- Quantitative description of fluid flows produced by left-right cilia in zebrafish / Craig Fox, M. Lisa Manning, Jeffrey D. Amack -- Efficient live fluorescence imaging of intraflagellar transport in mammalian primary cilia / Hiroaki Ishikawa, Wallace F. Marshall -- Analysis of soluble protein entry into primary cilia using semipermeabilized cells / David K. Breslow, Maxence V. Nachury -- Methods for imaging individual cilia in living echinoid embryos / Robert L. Morris, Hans W. Pope, Adam N. Sholi, Leah M. Williams, Chelsea R. Ettinger, Gwendolyn M. Beacham, Tatsushi Shintaku, Zachary D. Abbott, Elyse M. Doherty -- The planarian Schmidtea mediterranea as a model for studying motile cilia and multiciliated cells / Cyril Basquin, Anne-Marie Orfila, Juliette Azimzadeh -- Quantitative analysis of flagellar proteins in Drosophila sperm tails / Teresa Mendes Maia, Perrine Paul-Gilloteaux, Renata Basto -- Imaging cilia in Drosophila melanogaster / Jennifer Vieillard, Jean-Luc Duteyrat, Elisabeth Cortier, Benedicte Durand -- Studying G protein-coupled receptors: Immunoblotting, immunoprecipitation, phosphorylation, surface labeling, and cross-linking protocols / Kasturi Pal, Hemant Badgandi, Saikat Mukhopadhyay -- Image analysis of Caenorhabditis elegans ciliary transition zone structure, ultrastructure, molecular composition and function / Anna A. W. M. Sanders, Julie Kennedy, Oliver E. Blacque -- Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii / Huawen Lin, Susan K. Dutcher -- Axonemal motility in Chlamydomonas / Ken-ichi Wakabayashi, Ritsu Kamiya -- Posttranscriptional control over rapid development and ciliogenesis in marsilea / Stephen M. Wolniak, Thomas C. Boothby, Corine M. van der Weele -- Total internal reflection fluorescence microscopy of intraflagellar transport in tetrahymena thermophila / Yu-Yang Jiang, Karl Lechtreck, Jacek Gaertig -- Ciliary heterogeneity within a single cell : the paramecium model / Anne Aubusson-Fleury, Jean Cohen, Michel Lemullois -- Imaging intraflagellar transport in trypanosomes / Julien Santi-Rocca, Nicolas Chenouard, Cécile Fort, Thibault Lagache, Jean-Christophe Olivo-Marin, Philippe Bastin -- Scanning and three-dimensional electron microscopy methods for the study of trypanosoma brucei and leishmania mexicana flagella / Eva Gluenz, Richard John Wheeler, Louise Hughes, Sue Vaughan.
  • Digital
    edited by Robert P. Mecham.
    ScienceDirect2018
  • Digital
    edited by Thomas Weimbs.
    ScienceDirectPt A.
    ScienceDirectPt. B
  • Digital/Print
    edited by Bhanu P. Jena.
    Digital : ScienceDirect2008
    Print2008
  • Digital
    edited by Stuart C. Feinstein and Nichole E. Lapointe.
    ScienceDirect2017
    Methods in Tau Cell Biology, Volume 141, the latest release in the Methods in Cell Biology series, looks at methods involved in tau cell biology. Edited by leaders in the field, this volume provides proven, state-of-art techniques and relevant historical background and theory that aids researchers with tactics for efficient design and effective implementation of experimental methodologies. Topics of note in this updated volume include sections on Recombinant tau expression and purification, In vitro MT dynamics and MT ends, Methods related to investigating tau structure and MT bundling, Neurite outgrowth and retraction, and Methods related to studying tau fragmentation.
  • Digital
    edited by Junsang Doh, Daniel Fletcher, Matthieu Piel.
    ScienceDirect2018
  • Digital
    edited by Matthiew Piel, Daniel Fletcher, Junsang Doh.
    ScienceDirect2018
  • Digital
    edited by Daniel A. Fletcher, Junsang Doh, Matthieu Piel.
    ScienceDirect2018
    Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. Contains contributions from experts in the field from across the world. Covers a wide array of topics on both mitosis and meiosisIncludes relevant, analysis based topics.
  • Digital
    edited by David J. Carroll.
    Springer2009
    Expression of exogenous mRNA in Xenopus laevis embryos for the study of cell cycle regulation / Jill C. Sible and Brian N. Wroble -- Use of luciferase chimaera to monitor PLC[zeta] expression in mouse eggs / Karl Swann ... [et al.] -- Analysis of 14-3-3 family member function in Xenopus embryos by microinjection of antisense morpholino oligos / Jeffrey M.S. Lau and Anthony J. Muslin -- A microinjectable biological system, the Xenopus oocyte, as an approach to understanding signal transduction protein function / Katia Cailliau and Edith Browaeys-Poly -- Combining microinjection and immunoblotting to analyse MAP kinase phosphorylation in single starfish oocytes and eggs / David J. Carroll and Wei Hua -- Analysis of signaling pathways in zebrafish development by microinjection / William H. Kinsey -- Protein inhibition by microinjection and RNA-mediated interference in tissue culture cells : complementary approaches to study protein function / Jane R. Stout, Rania S. Rizk, and Claire E. Walczak -- DNA delivery by microinjection for the generation of recombinant mammalian cell lines / Sebastien Chenuet ... [et al.] -- Bacteriophage [phi]C31 integrase mediated transgenesis in Xenopus laevis for protein expression at endogenous levels / Bryan G. Allen and Daniel L. Weeks -- Germline transformation of Caenorhabditis elegans by injection / Pavan Kadandale, Indrani Chatterjee, and Andrew Singson -- Quantitative microinjection of mouse oocytes and eggs / Douglas Kline -- Microinjection of follicle-enclosed mouse oocytes / Laurinda A. Jaffe ... [et al.] -- Functional studies of regulatory genes in the sea urchin embryo / Vincenzo Cavalieri, Maria Di Bernardo, and Giovanni Spinelli -- Exploring the cytoskeleton during intracytoplasmic sperm injection in humans / Vanesa Y. Rawe and Héctor Chemes -- Somatic cell nuclear transfer in the mouse / Satoshi Kishigami and Teruhiko Wakayama.
  • Digital
    edited by Matthieu Piel and Manuel Théry.
    ScienceDirectPt. A
    ScienceDirectPt. B
    ScienceDirectPt. C
    "Micropatterning refers generally to techniques which provide an experimental control over the chemical, physical, or geometrical properties of materials at the micron or submicron scale, and are thus used to produce spatial patterns of these properties. These techniques, which were often originally designed for application in microelectronics, have spread over most areas of science, including biology. They have proved particularly useful for cell biology, bridging the gap between the Petri-dish and complex 3D assays and tissues."--Preface.
  • Digital/Print
    Gaetano Santulli, editor.
    Digital : Springer2015
    Print2015
    1. A fleeting glimpse inside microRNA, epigenetics and micropeptidomics -- 2. The microRNA machinery -- 3. microRNAs in mitochondria: an unexplored niche -- 4. microRNAs distinctively regulate vascular smooth muscle and endothelial cells: functional implications in angiogenesis, atherosclerosis, and in-stent restenosis -- 5. Mechanistic role of microRNAs in coupling lipid metabolism and atherosclerosis -- 6. microRNAs in pancreatic β-cell physiology -- 7. microRNA and cardiac regeneration -- 8. microRNAs and endometrial pathophysiology -- 9. microRNA and NF-kappa B -- 10. microRNAs: key players in hematopoiesis -- 11. Regulatory roles of miRNAs in aging -- 12. Computational prediction of microRNA targets
  • Digital
    Zhiguo Wang, Baofeng Yang.
    Springer2010
  • Digital
    Zhiguo Wang.
    Springer2009.
  • Digital/Print
    Catherine M. Greene, editor.
    Digital : Springer2015
    Print2015
    The book serves as a comprehensive resource for scientists and clinicians studying the role of non-coding RNAs in inflammation (viral infections, wound inflammation), human inflammatory diseases (i.e. rheumatoid arthritis, Crohnℓ́ℓs disease, diabetes), and innate immunity. It provides a universal reference work comprising both basic and specialized information. Given that ncRNAs represent new therapeutic targets, this volume will also be of interest to industrial biomedical researchers and those involved in drug development.
  • Digital
    edited by Tamas Dalmay.
    Springer2011
    In situ detection of microRNAs in animals / Dylan Sweetman -- Detection of microRNAs in plants by in situ hybridisation / Eva Varallyay and Zoltan Havelda -- Detecting sRNAs by northern blotting / Sara Lopez-Gomollon -- Profiling microRNAs by real-time PCR / Nanna Jacobsen, Ditte Andreasen, and Peter Mouritzen -- Detection of small RNAs and microRNAs using deep sequencing technology / Ericka R. Havecker -- Detection of microRNAs in prostate cancer cells by mircoRNA array / Xiaoqing Tang [and others] -- MicroRNA knock down by cholesterol-conjugated antisence oligos in mouse organ culture / Sharon Kredo-Russo and Eran Hornstein -- Protocols for use of homologous recombination gene targeting to produce microRNA mutants in drosophila / Ya-Wen Chen, Ruifen Weng, and Stephen M. Cohen -- Engineering elements for gene silencing : the artificial microRNAs technology / Pablo Andres Manavella and Ignacio Rubio-Somoza -- Mimicry technology : suppressing small RNA activity in plants / Ignacio Rubio-Somoza and Pablo Andres Manavella -- Experimental validation of microRNA targets using a luciferase reporter system / Francisco E. Nicolas -- Experimental identification of microRNA targets by immunoprecipitation of argonaute protein complexes / Michaela Beitzinger and Gunter Meister -- Comprehensive identification of miRNA target sites in live animals / Dimitrios G. Zisoulis, Gene W. Yeo, and Amy E. Pasquinelli -- Target validation of plant microRNAs / Cesar Llave [and others] -- High-throughput sequencing-based methodology to identify all uncapped and cleaved RNA molecules in eukaryotic genomes / Matthew W. Endres, Rebecca T. Cook, and Brian D. Gregory.
  • Digital
    edited by Anne Straube.
    Springer2011
    How to measure microtubule dynamics? / Anne Straube -- Purification of tubulin from porcine brain / Christopher Gell [and others] -- Purification of tubulin from the fission yeast Schizosaccharomyces pombe / Douglas R. Drummond [and others] -- Generation of differentially polyglutamylated microtubules / Benjamin Lacroix and Carsten Janke -- Preparation of pure tyrosinated or detyrosinated tubulin isoforms / Laurence Lafanechère and Didier Job -- [alpha][beta]-tubulin and microtubule-binding assays / Jaime N. Campbell and Kevin C. Slep -- Analysis of microtubule assembly kinetics using turbidimetry / Felicia Gaskin -- Isolation of centrosomes from cultured cells / Simone Reber -- Preparation of dual-color polarity-marked fluorescent microtubule seeds / Miho Katsuki, Etsuko Muto, and Robert A. Cross -- Reconstitution and quantification of dynamic microtubule end tracking in vitro using TIRF microscopy / Ivo A. Telley, Peter Bieling, and Thomas Surrey -- Force generation by dynamic microtubules in vitro / Svenja-Marei Kalisch, Liedewij Laan, and Marileen Dogterom -- Variations on the single-molecule assay for microtubule-associated proteins and kinesins / Susanne Bechstedt [and others] -- Analysing the ATP turnover cycle of microtubule motors / Claire T. Friel, Clive R. Bagshaw, and Jonathon Howard -- Cryo-electron tomography of microtubules assembled in vitro from purified components / Frédéric M. Coquelle [and others] -- Kinetochore microtubules, analyzed by correlated light and immunoelectron microscopy / Andreas Merdes and Jan De Mey -- Photoactivatable-GFP-[alpha]-tubulin as a tool to study microtubule plus-end turnover in living human cells / Catarina P. Samora and Andrew D. McAinsh -- Quantification of asymmetric microtubule nucleation at subcellular structures / Xiaodong Zhu and Irina Kaverina -- Investigating microtubule dynamic instability using microtubule-targeting agents / Stéphane Honore and Diane Braguer -- Laser ablation of the microtubule cytoskeleton : setting up and working with an ablation system / Nicola Maghelli and Iva M. Tolić-Nørrelykke -- Isolation, identification, and validation of microtubule-associated proteins from Drosophila embryos / Robin Antrobus and James G. Wakefield -- Isolation of novel +TIPs and their binding partners using affinity purification techniques / Ka Lou Yu [and others].
  • Digital
    edited by John J. Correia, Leslie Wilson.
    ScienceDirect2013
    Site-specific fluorescent labeling of tubulin / Kamalika Muhkerjee, Susan L. Bane -- Measuring microtubule persistence length using a microtubule gliding assay / Douglas S. Martin -- Structural studies of the doublecortin family of MAPs / Frank Fourniol, Mylène Perderiset, Anne Houdusse, Carolyn Moores -- Detection and quantification of microtubule detachment from centrosomes and spindle poles / Anutosh Ganguly, Hailing Yang, Fernando Cabral -- Regulation of tubulin expression by micro-RNAs : implications for drug resistance / Sharon Lobert, Mary E. Graichen -- Determining the structure-mechanics relationships of dense microtubule networks with confocal microscopy and magnetic tweezers-based microrheology / Yali Yang, Megan T. Valentine -- Studying mitochondria and microtubule localization and dynamics in standardized cell shapes / Andrea Pelikan, James Sillibourne, Stephanie Miserey-Lenkei, Frederique Carlier-Grynkorn, Bruno Goud, Phong T. Tran -- Going solo : measuring the motions of microtubules with an in vitro assay for TIRF microscopy / Kris Leslie, Niels Galjart -- Analysis of microtubules in isolated axoplasm from the squid giant axon / Yuyu Song, Scott T. Brady -- Imaging GTP-bound tubulin : from cellular to in vitro assembled microtubules / Hélène de Forges, Antoine Pilon, Christian Poüs, Franck Perez -- Tubulin-specific chaperones : components of a molecular machine that assembles the ... heterodimer / Guoling Tian, Nicholas J. Cowan -- Heterotrimeric G proteins and microtubules / Witchuda Saengsawang, Mark M. Rasenick -- Purification and biophysical analysis of microtubule-severing enzymes in vitro / Juan Daniel Diaz-Valencia, Megan Bailey, Jennifer L. Ross -- Measurement of in vitro microtubule polymerization by turbidity and fluorescence / Matthew Mirigian, Kamalika Mukherjee, Susan L. Bane, Dan L. Sackett -- Live-cell imaging of microtubules and microtubule-associated proteins in arabidopsis thaliana / Jessica Lucas -- Investigating tubulin posttranslational modifications with specific antibodies / Maria M. Magiera, Carsten Janke -- Purification and assembly of bacterial tubulin BtubA/B and constructs bearing eukaryotic tubulin sequences / José M. Andreu, María A. Oliva -- Microtubule-associated proteins and tubulin interaction by isothermal titration calorimetry / P.O. Tsvetkov, P. Barbier, G. Breuzard, V. Peyrot, F. Devred -- Methods for studying microtubule binding site interactions : zampanolide as a covalent binding agent / Jessica J. Field, Enrique Calvo, Peter T. Northcote, John H. Miller, Karl-Heinz Altmann, José Fernando Díaz -- Studying kinetochore-fiber ultrastructure using correlative light-electron microscopy / Daniel G. Booth, Liam P. Cheeseman, Ian A. Prior, Stephen J. Royle -- Fluorescence-based assays for microtubule architecture / Susanne Bechstedt, Gary L. Brouhard -- Structure-function analysis of yeast tubulin / Anna Luchniak, Yusuke Fukuda, Mohan L. Gupta -- Using MTBindingSim as a tool for experimental planning and interpretation / Julia T. Philip, Aranda R. Duan, Emily O. Alberico, Holly V. Goodson -- Imaging individual spindle microtubule dynamics in fission yeast / Judite Costa, Chuanhai Fu, Viktoriya Syrovatkina, Phong T. Tran.
  • Digital
    edited by Leslie Wilson, John J. Correia.
    ScienceDirect2010
    sect. I. Isolation and biochemistry of tubulin and characterization of antibodies and isotypes -- Preparation of microtubule protein and purified tupbulin from bovine brain by cycles of assembly and disassembly and phosphocellulose chromatography / Herbert P. Miller, Leslie Wilson -- Isolating tubulin from nonneural sources / Dan L. Sackett, Karl A. Werbovetz, Naomi S. Morrissette -- Characterization of anti-B-tubulin antibodies / Anthony J. Spano, Anthony Frankfurter -- Expression profliing of tubulin isotypes and microtubule-interacting proteins using real-time polymerase chain reaction / Sharon Lobert, Laree Hiser, John J. Correia -- Nondenaturing electrophoresis as a tool to investigate tabulin complexes / Monica Lopez Fanarago... [et al.] -- Mass spectrometry analysis of c-terminal postranslational modifications of tubulins / Virginie Redeker -- Methods in tubulin proteomics / Leah M. Miller... [et al.] -- sect. II. Microtubule structure and dynamics -- Cryo-em studies of microtubule structural intermediates and kinetochore-microtubule interactions / Eva Nogales, Vincent H. Rame, Hong-Wei Wang -- High-resolution imaging of microtubules and cytoskeleton structures by atomic force microscopy / Loic Hamon, Patrick A. Curmi, David Patre -- Using computational modeling to understand microtubule dynamics : a primer for cell biologists / Holly V. Goodson, Ivan V. Gregoretti -- Analysis of dynamic instability of steady-state microtubules in vitro by video-enchanced differential interference contrast microscopy with an appendix by Emin Oroudjev / Mythili Yenjerla, Manu Lopus, Leslie Wilson -- Nanometer-resolution microtubule polymerization assays using optical tweezers and microfabricated barriers/ Blake d. Charlebois, Henry T. Schek III, Alan J. Hunt -- Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy / Christopher Gell... [et al.] -- Studying kinesin motors by optical 3D-nanometry in gliding motility assays / Bert Nitzsche... [et al.] -- sect. III. Drugs -- Analysis of tubulin oligomers by analytical ultricentrifugation / John J. Correia -- Determination of drug binding to mactrotubules in vitro / Jennifer A. Smith, Mary Ann Jordan -- Fluorescence spectroscopic methods to analyze drug-tubulin interactoins / Bhabatarak Bhattacharyya, Sonia Kapoor, Dulal Panda -- Tubulin polymerizatin microassay used to compare ligand eficacy / Ashley Davis... [et al.] -- Fluorescent taxoid probes for microtubule research / Isabel Barasoain, J. Fernando Diaz, Jose M. Andrea -- Binding of vinca domain agents to tubulin : structural and biomedical studies / Anthony Cormier... [et al.] -- Measurement of ligand binding to tubulin by sulfhydryl reactivity / Adrian Begaye, Dan L. Sackett -- sect. IV. Interactions with motors and MAPs -- Probing interactions of tubulin with small molecules, peptides, and protein fragments by solution nuclear magnetic resonance / Marie-Jeanne Clement... [et al.] -- Microtubule and MAPs : thermodynamics of complex formation by AUC, ITC, fluorescence, and NMR / Francois Devred... [et al.] -- Quantitative analysis of MAP-mediated regulation of microtubule dynamic instability in vitro--focus on tau / Erkan Kiris, Donovan Ventimiglia, Stuart C. Feinstein -- Structure and dynamics of the kinesin-Microtubule interaction revealed by fluorescence polarization microscopy / Hernando Sosa, Ana B. Asenjo, Erwin J.G. Peterman -- Multiple collor single molecule TIRF imaging and tracking of MAPs and motors / Jennifer L. Ross, Ram Dixit -- Studying plus-end tracking at single molecule resolution using TIRF microscopy / Ram Dixit, Jennifer L. Ross -- Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor, and +TIP dynamics / Peter Bieling... [et al.] --
  • Digital
    edited by Lynne Cassimeris and Phong Tran.
    ScienceDirect2010
  • Digital
    Mine Ergüven, Takashi Muramatsu, Ayhan Bilir, editors.
    Springer2012
    pt. 1. GENERAL ASPECTS OF MIDKINE -- General Information on Midkine / Takashi Muramatsu -- The Role of Midkine in Neural Development and Patterning / Sheng Yao and Christoph Winkler -- Midkine: A Player in Innate Immunity / Sara L. Nordin and Arne Egesten -- Midkine/P63 Axis in Regulation of Epithelial-Mesenchymal Transition / Yiping Huang and Edward A. Ratovitski -- Immunochemical Methods for the Detection of Midkine in Samples of Human Origin / Stefan Sommerschuh, Michael Kirchner and Nicole Gliese -- Circulating Midkine in Malignant, Inflammatory, and Infectious Diseases: A Systematic Review / Malgorzata Krzystek-Korpacka and Malgorzata Matusiewicz -- pt. 2. MIDKINE AND INFLAMMATION -- Midkine in Inflammation and Inflammatory Diseases / Nuray Yazıhan, Mehtap Kacar Kocak and Ethem Akcıl -- Rheumatoid Arthritis and Midkine / Hisako Muramatsu, Kiyoko Maruyama and Naoki Ishiguro -- pt. 3. MIDKINE AND THE CARDIOVASCULAR SYSTEM -- Midkine and Heart Failure / Mitsuru Horiba and Kenji Kadomatsu -- Vascular Restenosis and Midkine / Kenji Kadomatsu -- The Potential of MK Through the Renin-Angiotensin System / Tomoki Kosugi and Kenji Kadomatsu -- pt. 4. MIDKINE AND THE NERVOUS SYSTEM -- Midkine and Multiple Sclerosis / Hideyuki Takeuchi -- Midkine Gene Transfer in Brain Infarction / Hiroaki Ooboshi, Eiichi Ishikawa, Junichi Takada, Takashi Shichita and Yasuhiro Kumai -- Midkine in Psychiatric and Neurodegenerative Diseases / Eiji Shimizu and Daisuke Matsuzawa -- pt. 5. MIDKINE AND THE UROGENITAL SYSTEM, REPRODUCTION AND AGING -- Role of Midkine in Nephrogenesis and Kidney Disease / Waichi Sato and Kenji Kadomatsu -- Midkine, a Factor Promoting Cytoplasmic Maturation of Oocytes / Masayasu Yamada, Yuuki Isaji and Shuntaro Ikeda -- Endometriosis May Be Promoted by Midkine: Possible Involvement of Ovarian Follicles / Yutaka Osuga, Yasushi Hirota, Kaori Koga, Osamu Yoshino and Tetsuya Hirata -- Stem Cells and Midkine / Hisako Muramatsu, Peng Zou and Takashi Muramatsu -- Midkine in Skeletal Physiology / Astrid Liedert, Thorsten Schinke, Anita Ignatius and Michael Amling -- pt. 6. MIDKINE AND CANCER -- Midkine and Chemoresistance in Cancers / Yoshifumi Takei and Kenji Kadomatsu -- A Gene Medicine with the Midkine-mediated Transcriptional Regulation as New Cancer Therapeutics / Masatoshi Tagawa, Kiyoko Kawamura, Ling Yu, Yuji Tada and Kenzo Hiroshima, et al. -- Midkine as a Tumor-Shared Antigen / Jerome Kerzerho, Florence Anne Castelli and Bernard Maillère -- Midkine in Prostate Cancer / Zongbing You -- Midkine Is a Potential Therapeutic Target of Hepatocellular Carcinoma / Li Cheng Dai, Xing Yao and Jing Zhong -- Midkine in Glioblastoma with the Modulator Role Switching Autophagy to Cell Death or Cell Survival / Mine Ergüven and Ayhan Bilir -- Colon Cancer and Midkine / Özgür Kemik, Ahu Sarbay Kemik and A. Cumhur Dülger -- Osteosarcoma and Midkine / Tadashi Kaname, Kumiko Yanagi and Hiroki Maehara.
  • Digital
    H. Tedeschi.
    Springer1976
  • Digital
    edited by Liza A. Pon, Eric A. Schoon.
    ScienceDirect2020
  • Digital
    edited by Dejana Mokranjac, Fabiana Perocchi.
    Springer2017
    Guide to computational methods for predicting mitochondrial localization / Su Sun and Bianca H. Habermann -- Isolation of functional mitochondria from cultured cells and mouse tissues / Jennifer Wettmarshausen and Fabiana Perocchi -- Isolation of mitochondria from Saccharomyces cerevisiae / Toshiaki Izawa and Ann-Katrin Unger -- Isolation of contact sites between inner and outer mitochondrial membranes / Max Harner -- Isolation of mitochondria-associated membranes (MAM) from mouse brain tissue / Bernadette Schreiner and Maria Ankarcrona -- Label-free quantitative analysis of mitochondrial proteomes using the multienzyme digestion-filter aided sample preparation (MED-FASP) and "Total Protein Approach" / Jacek R. Wiśniewski -- Quantitative analysis of glycerophospholipids in mitochondria by mass spectrometry / Takashi Tatsuta -- Detection of cysteine redox states in mitochondrial proteins in intact mammalian cells / Markus Habich and Jan Riemer -- Chemical crosslinking in intact mitochondria / Rupa Banerjee, Umut Günsel, and Dejana Mokranjac -- Reconstitution of mitochondrial membrane proteins into nanodiscs by cell-free expression / Ketan Malhotra and Nathan N. Alder -- Detection of dual targeting and dual function of mitochondrial proteins in yeast / Reut Ben-Menachem and Ophry Pines -- Localizing mRNAs encoding mitochondrial proteins in yeast by fluorescence microscopy and subcellular fractionation / Dmitry Zabezhinsky, Hannah Sperber, and Jeffrey E. Gerst -- Assessing mitochondrial bioenergetics in isolated mitochondria from various mouse tissues using seahorse XF96 analyzer / Arcangela Iuso, Birgit Repp, Caroline Biagosch, Caterina Terrile, and Holger Prokisch -- Application of FRET-based biosensor "ATeam" for visualization of ATP levels in the mitochondrial matrix of living mammalian cells / Tomoki Yoshida, Soaad Alfaqaan, Norio Sasaoka, and Hiromi Imamura -- Microplate-based bioluminescence assay of mitochondrial calcium uptake / María Teresa Alonso, Paloma Navas-Navarro, and Javier García-Sancho -- New imaging tools to analyze mitochondrial morphology in Caenorhabditis elegans / Saroj G. Regmi and Stéphane G. Rolland -- Single molecule tracking and localization of mitochondrial protein complexes in live cells / Timo Appelhans and Karin Busch -- Analysis of yeast mitochondria by electron microscopy / Ann-Katrin Unger, Stefan Geimer, Max Harner, Walter Neupert, and Benedikt Westermann -- Analysis of mitochondrial membrane protein complexes by electron cryo-tomography / Vicki A.M. Gold, Tobias Brandt, Laetitia Cavellini, Mickael M. Cohen, Raffaele Ieva, and Martin van der Laan -- Assays for Mitophagy in Yeast / Akinori Eiyama and Koji Okamoto -- Assessing Mitochondrial Selective Autophagy in the Nematode Caenorhabditis elegans / Konstantinos Palikaras and Nektarios Tavernarakis -- Assessing mitochondrial unfolded protein response in mammalian cells / Fiona Durand and Nicholas Hoogenraad -- Analysis of mitochondrial RNA-processing defects in patient-derived tissues by qRT-PCR and RNAseq / Robert Kopajtich, Johannes A. Mayr, and Holger Prokisch -- Identification of disease-causing mutations by functional complementation of patient-derived fibroblast cell lines / Laura S. Kremer and Holger Prokisch.
  • Digital
    Keshav K. Singh, Leslie C. Costello, editors.
    Springer2009
  • Digital
    edited by Carlos M. Palmeira, António J. Moreno.
    Springer2012
    Overview of mitochondrial bioenergetics / Vitor M.C. Madeira -- Evaluation of respiration with Clark type electrode in isolated mitochondria and permeabilized animal cells / Ana M. Silva and Paulo J. Oliveira -- High-resolution respirometry : OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle / Dominik Pesta and Erich Gnaiger -- High-throughput analysis of mitochondrial oxygen consumption / James Hynes, Rachel L. Swiss, and Yvonne Will -- Modulation of cellular respiration by endogenously produced nitric oxide in rat hippocampal slices / Ana Ledo, R.M. Barbosa, and J. Laranjinha -- Mitochondrial membrane potential ([delta][psi]) fluctuations associated with the metabolic states of mitochondria / Carlos M. Palmeira and Anabela P. Rolo -- Safranine as a fluorescent probe for the evaluation of mitochondrial membrane potential in isolated organelles and permeabilized cells / Tiago R. Figueira ... [et al.] -- Fluorescence measurement of mitochondrial membrane potential changes in cultured cells / David G. Nicholls -- Phenomenological kinetic and control analysis of oxidative phosphorylation in isolated mitochondria / Vilmante Borutaite and Rasa Baniene -- Expression of uncoupling proteins in a mammalian cell culture system (HEK293) and assessment of their protein function / Martin Jastroch -- Measurement of proton leak and electron leak in isolated mitochondria / Charles Affourtit, Casey L. Quinlan, and Martin D. Brand -- Relation between mitochondrial membrane potential and ROS formation / Jan M. Suski ... [et al.] -- Use of a calcium-sensitive electrode for studies on mitochondrial calcium transport / António J.M. Moreno and Joaquim A. Vicente -- Imaging mitochondrial calcium signalling with fluorescent probes and single or two photon confocal microscopy / Sean M. Davidson and Michael R. Duchen -- Mitochondrial permeability transition pore and calcium handling / Renee Wong, Charles Steenbergen, and Elizabeth Murphy -- Imaging of mitochondrial pH using SNARF-1 / Venkat K. Ramshesh and John J. Lemasters -- Redox equivalents and mitochondrial bioenergetics / James R. Roede, Young-Mi Go, and Dean P. Jones -- NMR methodologies for studying mitochondrial bioenergetics / Tiago C. Alves, Ivana Jarak, and Rui A. Carvalho -- Computational modeling of mitochondrial function / Sonia Cortassa and Miguel A. Aon.
  • Digital
    edited by Carlos M. Palmeira and António J. Moreno.
    Springer2018
    Overview of mitochondrial bioenergetics / Vitor M. C. Madeira -- Evaluation of respiration with clark-type electrode in isolated mitochondria and permeabilized animal cells / Ana M. Silva and Paulo J. Oliveira -- High-resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated Mitochondria / Carolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, Andras T. Meszaros, and Erich Gnaiger -- High-throughput analysis of mitochondrial oxygen consumption / James Hynes, Rachel L. Swiss, and Yvonne Will -- Modulation of cellular respiration by endogenously produced nitric oxide in rat hippocampal slices / Ana Ledo, Rui M. Barbosa, and Joao Laranjinha -- Mitochondrial membrane potential (ΔΨ) fluctuations associated with the metabolic states of mitochondria / Joao Soeiro Teodoro, Carlos Marques Palmeira, and Anabela Pinto Rolo -- Fluorescence measurement of mitochondrial membrane potential changes in cultured cells / David G. Nicholls -- Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production / Timea Koml odi, Ondrej Sobotka, Gerhard Krumschnabel, Nicole Bezuidenhout, Elisabeth Hiller, Carolina Doerrier, and Erich Gnaiger -- Measurement of proton leak in isolated mitochondria / Charles Affourtit, Hoi-Shan Wong, and Martin D. Brand -- Imaging mitochondrial calcium fluxes with fluorescent probes and single- or two-photon confocal microscopy / Sean M. Davidson and Michael R. Duchen -- Mitochondrial permeability transition pore and calcium handling / Randi J. Parks, Elizabeth Murphy, and Julia C. Liu -- Redox equivalents and mitochondrial bioenergetics / James R. Roede, Young-Mi Go, and Dean P. Jones -- Mitochondrial bioenergetics by 13C-NMR isotopomer analysis / Rui A. Carvalho and Ivana Jarak -- Computational modeling of mitochondrial function from a systems biology perspective / Sonia Cortassa, Steven J. Sollott, and Miguel A. Aon -- Monitoring the mitochondrial dynamics in mammalian cells / Luca Simula and Silvia Campello -- Plate-based measurement of superoxide and hydrogen peroxide production by isolated mitochondria / Hoi-Shan Wong, Pierre-Axel Monternier, Adam L. Orr, and Martin D. Brand -- Plate-based measurement of respiration by isolated mitochondria / Shona A. Mookerjee, Casey L. Quinlan, Hoi-Shan Wong, Pratiksha Dighe, and Martin D. Brand -- Detection of iron depletion- and hypoxia-induced mitophagy in mammalian cells / Shun-Ichi Yamashita and Tomotake Kanki -- Importance of calcium ions for determining mitochondrial glycerol-3-phosphate dehydrogenase activity when measuring uncoupling protein 1 (UCP1) function in mitochondria isolated from brown adipose tissue / Kieran J. Clarke and Richard K. Porter -- Isolation and analysis of mitochondrial small RNAs from rat liver tissue and HepG2 cells / Julian Geiger and Louise T. Dalgaard -- Imaging of mitochondrial pH using SNARF-1 / Venkat K. Ramshesh and John J. Lemasters -- Relation between mitochondrial membrane potential and ROS formation / Jan Suski, Magdalena Lebiedzinska, Massimo Bonora, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski -- Assessing spatiotemporal and functional organization of mitochondrial networks / Felix T. Kurz, Miguel A. Aon, Brian O'Rourke, and Antonis A. Armoundas -- Measurement of mitochondrial ROS formation / Soni Deshwal, Salvatore Antonucci, Nina Kaludercic, and Fabio Di Lisa.
  • Digital
    edited by Lee-Jun C. Wong.
    Springer2012
    Mitochondrial DNA mutations : an overview of clinical and molecular aspects / William J. Craigen -- Nuclear gene defects in mitochondrial disorders / Fernando Scaglia -- Diagnostic challenges of mitochondrial disorders : complexities of two genomes / Brett H. Graham -- Biochemical analyses of the electron transport chain complexes by spectrophotometry / Ann E. Frazier and David R. Thorburn -- Measurement of mitochondrial oxygen consumption using a Clark electrode / Zhihong Li and Brett H. Graham -- Mitochondrial respiratory chain : biochemical analysis and criterion for deficiency in diagnosis / Manuela M. Grazina -- Assays of pyruvate dehydrogenase complex and pyruvate carboxylase activity / Douglas Kerr, George Grahame, and Ghunwa Nakouzi -- Assessment of thymidine phosphorylase function : measurement of plasma thymidine (and deoxyuridine) and thymidine phosphorylase activity / Ramon Martí, Luis C. López, and Michio Hirano -- Measurement of mitochondrial dNTP pools / Ramon Martí, Beatriz Dorado, and Michio Hirano -- Measurement of oxidized and reduced coenzyme Q in biological fluids, cells, and tissues : an HPLC-EC method / Peter H. Tang and Michael V. Miles -- Assay to measure oxidized and reduced forms of CoQ by LC-MS/MS / Si Houn Hahn, Sandra Kerfoot, and Valeria Vasta -- Morphological assessment of mitochondrial respiratory chain function on tissue sections / Kurenai Tanji -- Blue native polyacrylamide gel electrophoresis : a powerful diagnostic tool for the detection of assembly defects in the enzyme complexes of oxidative phosphorylation / Scot C. Leary -- Radioactive labeling of mitochondrial translation products in cultured cells / Florin Sasarman and Eric A. Shoubridge -- Transmitochondrial cybrids : tools for functional studies of mutant mitochondria / Sajna Antony Vithayathil, Yewei Ma, and Benny Abraham Kaipparettu -- Fluorescence-activated cell sorting analysis of mitochondrial content, membrane potential, and matrix oxidant burden in human lymphoblastoid cell lines / Stephen Dingley, Kimberly A. Chapman, and Marni J. Falk -- Molecular profiling of mitochondrial dysfunction in Caenorhabditis elegans / Erzsebet Polyak, Zhe Zhang, and Marni J. Falk -- Analysis of common mitochondrial DNA mutations by allele-specific oligonucleotide and southern blot hybridization / Sha Tang [and others] -- Sequence analysis of the whole mitochondrial genome and nuclear genes causing mitochondrial disorders / Megan L. Landsverk, Megan E. Cornwell, and Meagan E. Palculict -- Utility of array CGH in molecular diagnosis of mitochondrial disorders / Jing Wang and Mrudula Rakhade -- Quantification of mtDNA mutation heteroplasmy (ARMS qPCR) / Victor Venegas and Michelle C. Halberg -- Measurement of mitochondrial DNA copy number / Victor Venegas and Michelle C. Halberg -- Determination of the clinical significance of an unclassified variant / Victor Wei Zhang and Jing Wang.
  • Digital
    Lee-Jun C. Wong, editor.
    Springer2013
    Mitochondrial cytopathies are mutations in the inherited maternal mitochondrial genome, or the nuclear DNA-mutation. Mitochondrial respiratory chain disorders (RCD) are a group of genetically and clinically heterogeneous diseases, due to the fact that protein components of the respiratory chain are encoded by both mitochondrial and nuclear genomes and are essential in all cells. In addition, the biogenesis, structure and function of mitochondria, including DNA replication, transcription, and translation, all require nuclear encoded genes. Since mitochondria are present in every cell, every tissue, mitochondrial disorders usually affects multiple organs. Mitochondrial Disorders Caused by Nuclear Genes discusses the biochemical, molecular, clinical, and genetic aspects of complex dual genome mitochondrial disorders. Chapters include genes involved in mitochondrial DNA biogenesis and maintenance of mitochondrial DNA integrity, complex subunits and assembly genes, and mitochondrial protein translation related diseases.
  • Digital
    Justin C. St. John, editor.
    Springer2013
    1. Clinical Approach to the Diagnosis of Mitochondrial Disease / Dominic Thyagarajan, p. 1-23 -- 2. Mitochondrial DNA Mutations and Their Effects on Complex I Biogenesis: Implications for Metabolic Disease / Matthew McKenzie, p. 25-47 -- 3. Embryonic Stem Cells: A Signalling Perspective / Marios P. Stavridis, p. 49-68 -- 4. From Oocytes and Pluripotent Stem Cells to Fully Differentiated Fates: (Also) a Mitochondrial Odyssey / João Ramalho-Santos, Ana Sofia Rodrigues, p. 69-86 -- 5. From Pluripotency to Differentiation: The Role of mtDNA in Stem Cell Models of Mitochondrial Diseases / João Facucho-Oliveira, Tejal Kulkarni, p. 87-118 -- 6. The Role of Mitochondrial DNA in Tumorigenesis / Ka Yu Yeung, Adam Dickinson, p. 119-155 -- 7. Assisted Reproductive Technologies: The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next / Richard D. W. Kelly, Arsalan Mahmud, p. 157-183.
  • Digital
    edited by Yvonne Will, James A. Dykens.
    Wiley2018
    Mitochondrial Dysfunction Caused by Drugs and Environmental Toxicants. Front Matter -- Contributions of Plasma Protein Binding and Membrane Transporters to Drug-Induced Mitochondrial Toxicity / Gavin P McStay -- The Role of Transporters in Drug Accumulation and Mitochondrial Toxicity / Kathleen M Giacomini, Huan-Chieh Chien -- Structure-Activity Modeling of Mitochondrial Dysfunction / Steve Enoch, Claire Mellor, Mark Nelms -- Mitochondria-Targeted Cytochromes P450 Modulate Adverse Drug Metabolism and Xenobiotic-Induced Toxicity / Haider Raza, F Peter Guengerich, Narayan G Avadhani -- Mitochondrial Dysfunction in Drug-Induced Liver Injury / Annie Borgne-Sanchez, Bernard Fromenty -- Evaluating Mitotoxicity as Either a Single or Multi-Mechanistic Insult in the Context of Hepatotoxicity / Amy L Ball, Laleh Kamalian, Carol E Jolly, Amy E Chadwick -- Cardiotoxicity of Drugs / Zoltan V Varga, Pal Pacher -- Skeletal Muscle Mitochondrial Toxicity / Eric K Herbert, Saul R Herbert, Karl E Herbert -- Manifestations of Drug Toxicity on Mitochondria in the Nervous System / Jochen H M Prehn, Irene Llorente-Folch -- Nephrotoxicity / Ana Belén Sanz, Maria Dolores Sanchez-Niño, Adrian M Ramos, Alberto Ortiz -- Mammalian Sperm Mitochondrial Function as Affected by Environmental Toxicants, Substances of Abuse, and Other Chemical Compounds / Sandra Amaral, Renata S Tavares, Sara Escada-Rebelo, Andreia F Silva, João Ramalho-Santos -- Biological and Computational Techniques to Identify Mitochondrial Toxicants / Robert B Cameron, Craig C Beeson, Rick G Schnellmann -- The Parallel Testing of Isolated Rat Liver and Kidney Mitochondria Reveals a Calcium-Dependent Sensitivity to Diclofenac and Ibuprofen / Sabine Schulz, Sabine Borchard, Tamara Rieder, Carola Eberhagen, Bastian Popper, Josef Lichtmannegger, Sabine Schmitt, Hans Zischka -- In Vitro Methodologies to Investigate Drug-Induced Toxicities / Rui F Simões, Teresa Cunha-Oliveira, Cláudio F Costa, Vilma A Sardão, Paulo J Oliveira -- Combined Automated Measurement of Respiratory Chain Complexes and Oxidative Stress / Marc Conti, Thierry Delvienne, Sylvain Loric -- Measurement of Mitochondrial Toxicity by Flow Cytometry / Padma Kumar Narayanan, Nianyu Li -- MitoChip / Varsha G Desai, G Ronald Jenkins -- Using 3D Microtissues for Identifying Mitochondrial Liabilities / Simon Messner, Olivier Frey, Katrin Rössger, Andy Neilson, Jens M Kelm -- Toward Mitochondrial Medicine / David A Dunn, Michael H Irwin, Walter H Moos, Kosta Steliou, Carl A Pinkert -- Measurement of Oxygen Metabolism In Vivo / M P J Diemen, R Ubbink, F M Münker, E G Mik, G J Groeneveld -- Detection of Mitochondrial Toxicity Using Zebrafish / Sherine S L Chan, Tucker Williamson -- MiRNA as Biomarkers of Mitochondrial Toxicity / Terry R Vleet, Prathap Kumar Mahalingaiah -- Biomarkers of Mitochondrial Injury After Acetaminophen Overdose / Benjamin L Woolbright, Hartmut Jaeschke -- Acylcarnitines as Translational Biomarkers of Mitochondrial Dysfunction / Richard D Beger, Sudeepa Bhattacharyya, Pritmohinder S Gill, Laura P James -- Mitochondrial DNA as a Potential Translational Biomarker of Mitochondrial Dysfunction in Drug-Induced Toxicity Studies / Afshan N Malik -- Predicting Off-Target Effects of Therapeutic Antiviral Ribonucleosides / Jamie J Arnold, Craig E Cameron -- Imaging of Mitochondrial Toxicity in the Kidney / Andrew M Hall, Joana R Martins, Claus D Schuh -- Imaging Mitochondrial Membrane Potential and Inner Membrane Permeability / Anna-Liisa Nieminen, Venkat K Ramshesh, John J Lemasters -- Quantifying Skeletal Muscle Mitochondrial Function In Vivo by 31P Magnetic Resonance Spectroscopy / Graham J Kemp -- Supplemental Images -- Front Matter -- Statin and Fibrate-Induced Dichotomy of Mitochondrial Function / Viruna Neergheen, Alex Dyson, Luke Wainwright, Iain P Hargreaves -- Friend or Foe / Sofia Annis, Adeel Safdar, Eduardo Biala, Ayesha Saleem, Housaiyin Li, Priya Gandhi, Zoe Fleischmann, Carmen Castaneda-Sceppa, Jonathan L Tilly, Dori C Woods, Konstantin Khrapko -- Involvement of Mitochondrial Dysfunction on the Toxic Effects Caused by Drugs of Abuse and Addiction / Daniel José Barbosa, João Paulo Capela, Maria Lourdes Bastos, Félix Carvalho -- Drug-Induced Mitochondrial Toxicity during Pregnancy / Diana Luz Juárez-Flores, Ana Sandra Hernández, Laura García-Otero, Mariona Guitart-Mampel, Marc Catalán-García, Ingrid González-Casacuberta, Jose César Milisenda, Josep Maria Grau, Francesc Cardellach, Constanza Morén, Glòria Garrabou -- Mitochondrial Toxicity in Children and Adolescents Exposed to Antiretroviral Therapy / Antoni Noguera-Julian, Eneritz Velasco-Arnaiz, Clàudia Fortuny -- Drug-Induced Mitochondrial Cardiomyopathy and Cardiovascular Risks in Children / Neha Bansal, Mariana Gerschenson, Tracie L Miller, Stephen E Sallan, Jason Czachor, Hiedy Razoky, Ashley Hill, Miriam Mestre, Steven E Lipshultz -- Role of Mitochondrial Dysfunction in Linezolid-Induced Lactic Acidosis / Alessandro Santini, Dario Ronchi, Daniela Piga, Alessandro Protti -- Metformin and Lactic Acidosis / Jean-Daniel Lalau -- Lessons Learned from a Phase I Clinical Trial of Mitochondrial Complex I Inhibition / Cecilia C Low Wang, Jeffrey L Galinkin, William R Hiatt -- Pharmacological Activation of Mitochondrial Biogenesis for the Treatment of Various Pathologies / Whitney S Gibbs, Natalie E Scholpa, Craig C Beeson, Rick G Schnellmann -- Mitochondrial Toxicity Induced by Chemotherapeutic Drugs / Luciana L Ferreira, Ana Raquel Coelho, Paulo J Oliveira, Teresa Cunha-Oliveira -- The Mitochondrial Exposome / Douglas I Walker, Kurt D Pennell, Dean P Jones -- Central Mitochondrial Signaling Mechanisms in Response to Environmental Agents / Young-Mi Go, Karan Uppal, Dean P Jones -- Detection of Mitochondrial Toxicity of Environmental Pollutants Using Caenorhabditis elegans / Laura L Maurer, Anthony L Luz, Joel N Meyer -- Persistent Organic Pollutants, Mitochondrial Dysfunction, and Metabolic Syndrome / Hong Kyu Lee, Youngmi Kim Pak -- Cigarette Smoke and Mitochondrial Damage / Jalal Pourahmad, Marjan Aghvami, Mohammad Hadi Zarei, Parvaneh Naserzadeh.
  • Digital
    Viswanathan Natarajan, Narasimham L. Parinandi, editors.
    Springer2014
    Mitochondria, often referred to as the 'powerhouses' of the cell, generate adenosine triphosphate (ATP) by oxidative phosphorylation or OXPHOS, and maintain cellular homeostasis. In addition to generating ATP, mitochondria are involved in regulation of cell cycle, proliferation, free radical production, innate immune responses and apoptosis. Mitochondrial Function in Lung Health and Disease fills the current gap in the literature and outlines the growing clinical relevance of mitochondrial dysfunction. Currently, there is no overview on the role of mitochondria in pulmonary diseases and this volume focuses on the mitochondrial metabolism, redox signaling, and mechanisms of mitochondrial pathways in lung injury, inflammation, repair and remodeling. Furthermore, in addition to their well-recognized role in cellular energy production and apoptosis, mitochondria appear to play a role in many respiratory diseases and lung cancer. Chapters are written by top notch researchers and clinicians and outline the evidence for mitochondrial biogenesis in inhalational lung injury, COPD and asthma.
  • Digital
    Anna Gvozdjáková, editor.
    Springer2008
  • Digital
    edited by Volkmar Weissig, Marvin Edeas.
    Springer2015
    This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. "Mitochondrial Medicine: Volume I, Probing Mitochondrial Function" focuses on methods being used for the assessment of mitochondrial function under physiological conditions as well as in healthy isolated mitochondria. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, "Mitochondrial Medicine" provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.
  • Digital
    edited by Volkmar Weissig, Marvin Edeas.
    Springer2015
    This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. "Mitochondrial Medicine: Volume II, Manipulating Mitochondrial Function" describes techniques developed for manipulating and assessing mitochondrial function under general pathological conditions and specific disease states. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, "Mitochondrial Medicine" provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.
  • Digital
    Bernhard Kadenbach, editor.
    Springer2012
    Introduction to Mitochondrial Oxidative Phosphorylation / Bernhard Kadenbach -- Mitochondrial Dynamics: The Intersection of Form and Function / Andrew Ferree and Orian Shirihai -- Biogenesis of Mitochondrial Proteins / Johannes M. Herrmann, Sebastian Longen, Daniel Weckbecker and Matthieu Depuydt -- Assembly Factors of Human Mitochondrial Respiratory Chain Complexes: Physiology and Pathophysiology / Daniele Ghezzi and Massimo Zeviani -- Supramolecular Organisation of the Mitochondrial Respiratory Chain: A New Challenge for the Mechanism and Control of Oxidative Phosphorylation / Giorgio Lenaz and Maria Luisa Genova -- Molecular Mechanisms of Superoxide Production by the Mitochondrial Respiratory Chain / Stefan Dröse and Ulrich Brandt -- Studies on the Function and Regulation of Mitochondrial Uncoupling Proteins / Richard K. Porter -- Evolution of the Couple Cytochrome c and Cytochrome c Oxidase in Primates / Denis Pierron, Derek E. Wildman, Maik Hüttemann, Thierry Letellier and Lawrence I. Grossman -- Reaction Mechanism of Mammalian Mitochondrial Cytochrome c Oxidase / Shinya Yoshikawa, Kazumasa Muramoto and Kyoko Shinzawa-Itoh -- Phosphorylation of Mammalian Cytochrome c and Cytochrome c Oxidase in the Regulation of Cell Destiny: Respiration, Apoptosis, and Human Disease / Maik Hüttemann, Icksoo Lee, Lawrence I. Grossman, Jeffrey W. Doan and Thomas H. Sanderson -- Individual Biochemical Behaviour Versus Biological Robustness: Spotlight on the Regulation of Cytochrome c Oxidase / Rabia Ramzan, Petra Weber, Bernhard Kadenbach and Sebastian Vogt -- Bigenomic Regulation of Cytochrome c Oxidase in Neurons and the Tight Coupling Between Neuronal Activity and Energy Metabolism / Margaret T. T. Wong-Riley -- Cytochrome c Oxidase and Its Role in Neurodegeneration and Neuroprotection / Susanne Arnold -- The Many Clinical Faces of Cytochrome c Oxidase Deficiency / Salvatore DiMauro, Kurenai Tanji and Eric A. Schon.
  • Digital
    edited by Carlos M. Palmeira, Anabela P. Rolo.
    Springer2015
    Isolation of crude mitochondrial fraction from cells / Mariusz R. Wieckowski and Lech Wojtczak -- Isolation of mitochondria from liver and extraction of total RNA and protein : analyses of MicroRNA and protein expressions / Pedro M. Borralho, Clifford J. Steer, and Cecília M.P. Rodrigues -- PCR based determination of mitochondrial DNA copy number in multiple species / John P. Rooney [and eight others] -- NAD⁺ content and its role in mitochondria / Wei Li and Anthony A. Sauve -- Measuring PGC-1[alpha] and its acetylation status in mouse primary myotubes / Ana P. Gomes and David A. Sinclair -- Measurement of mitochondrial oxygen consumption rates in mouse primary neurons and astrocytes / Sofia M. Ribeiro, Alfredo Giménez-Cassina, and Nika N. Danial -- Determination of oxidative phosphorylation complexes activities / João S. Teodoro, Carlos M. Palmeira, and Anabela P. Rolo -- Histoenzymatic methods for visualization of the activity of individual mitochondrial respiratory chain complexes in the muscle biopsies from patients with mitochondrial defects / Agnieszka Karkucinska-Wieckowska, Maciej Pronicki, and Mariusz R. Wieckowski -- A method aimed at assessing the functional consequences of the supramolecular organization of the respiratory electron transfer chain by time- resolved studies / Fabrice Rappaport -- BN-PAGE-based approach to study thyroid hormones and mitochondrial function / Elena Silvestri [and three others] -- Detection of UCP1 protein and measurements of dependent GDP-sensitive proton leak in non- phosphorylating thymus mitochondria / Kieran J. Clarke [and three others] -- Exploring liver mitochondrial function by ¹³C-stable isotope breath tests : implications in clinical biochemistry / Ignazio Grattagliano [and five others] -- Following mitochondria dynamism : confocal analysis of the organelle morphology / Francesca R. Mariotti, Mauro Corrado, and Silvia Campello -- Analysis of pro-apoptotic protein trafficking to and from mitochondria / Ignacio Vega-Naredo [and four others] -- Mitophagy and mitochondrial balance / Simone Patergnani and Paolo Pinton.
  • Digital
    edited by Nobutaka Hattori and Shinji Saiki.
    Springer2018
    Short Overview / Norihiko Furuya, pages 3-8 -- Induction of PINK1/Parkin-Mediated Mitophagy / Shigeto Sato, Norihiko Furuya, pages 9-17 -- Immunocytochemical Monitoring of PINK1/Parkin-Mediated Mitophagy in Cultured Cells / Motoki Fujimaki, Shinji Saiki, Yukiko Sasazawa, Kei-Ichi Ishikawa, Yoko Imamichi, Katsuhiko Sumiyoshi ... et al., pages 19-27 -- The Use of Correlative Light-Electron Microscopy (CLEM) to Study PINK1/Parkin-Mediated Mitophagy / Chieko Kishi-Itakura, Folma Buss, pages 29-39 -- Observation of Parkin-Mediated Mitophagy in Pancreatic β-Cells / Atsushi Hoshino, Satoaki Matoba, pages 41-46 -- Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila / Tsuyoshi Inoshita, Kahori Shiba-Fukushima, Hongrui Meng, Nobutaka Hattori, Yuzuru Imai, pages 47-57 -- Assessment of Mitophagy in iPS Cell-Derived Neurons / Kei-Ichi Ishikawa, Akihiro Yamaguchi, Hideyuki Okano, Wado Akamatsu, pages 59-67 -- Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting / Sachiyo Nagumo, Koji Okamoto, pages 71-83 -- MitoPho8Δ60 Assay as a Tool to Quantitatively Measure Mitophagy Activity / Zhiyuan Yao, Xu Liu, Daniel J. Klionsky, pages 85-93 -- Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants / Kentaro Furukawa, Tomotake Kanki, pages 95-104 -- Flow Cytometer Monitoring of Bnip3- and Bnip3L/Nix-Dependent Mitophagy / Matilda Šprung, Ivan Dikic, Ivana Novak, pages 105-110 -- Exploring MicroRNAs on NIX-Dependent Mitophagy / Wen Li, Hao Chen, Shupeng Li, Guanghong Lin, Du Feng, pages 111-121 -- Monitoring of Atg5-Independent Mitophagy / Satoko Arakawa, Shinya Honda, Satoru Torii, Masatsune Tsujioka, Shigeomi Shimizu, pages 125-132 -- Monitoring of Paternal Mitochondrial Degradation in Caenorhabditis elegans / Miyuki Sato, Ken Sato, pages 133-140 -- Detection of Hypoxia-Induced and Iron Depletion-Induced Mitophagy in Mammalian Cells / Shun-ichi Yamashita, Tomotake Kanki, pages 141-149 -- Monitoring Mitophagy During Aging in Caenorhabditis elegans / Nikolaos Charmpilas, Konstantinos Kounakis, Nektarios Tavernarakis, pages 151-160 -- Monitoring of Iron Depletion-Induced Mitophagy in Pathogenic Yeast / Koichi Tanabe, Minoru Nagi, pages 161-172 -- Dopaminergic Neuron-Specific Autophagy-Deficient Mice / Shigeto Sato, Nobutaka Hattori, pages 173-175.
  • Digital
    edited by David Sharp.
    Springer2014
    Analysis of Mitotic Protein Dynamics and Function in Drosophila Embryos by Live Cell Imaging and Quantitative Modeling -- Rapid Measurement of Mitotic Spindle Orientation in Cultured Mammalian Cells -- Automated Segmentation of the First Mitotic Spindle in Differential Interference Contrast Micro copy Images of C. elegans Embryos -- Imaging the Mitotic Spindle by Spinning Disk Microscopy in Tobacco Suspension Cultured Cells -- Image-Based Computational Tracking and Analysis of Spindle Protein Dynamics -- The Use of Cultured Drosophila Cells for Studying the Microtubule Cytoskeleton -- Measuring Microtubule Growth and Gliding in C. elegans Embryos -- XenopusEgg Extracts as a Simplified Model System for Structure Function Studies of Dynein Regulators -- Covalent Immobilization of Microtubules on Glass Surfaces for Molecular Motor Force Measurements and Other Single-Molecule Assays -- An Improved Optical Tweezers Assay for Measuring the Force Generation of Single Kinesin Molecules -- Seeded Microtubule Growth for Cryo-electron Microscopy of End Binding Proteins -- The Segmentation of Microtubules in Electron Tomograms using Amira -- Manipulating Cell Shape by Placing Cells into Micro-fabricated Chambers -- Four color FISH for the Detection of Low Levels Aneuploidy in Interphase Cells.
  • Digital
    edited by Helder Maiato, Melina Schuh.
    ScienceDirectPt. A
    ScienceDirectPt. B
  • Digital
    edited by Fernando Monje-Casas, Ethel Queralt.
    Springer2017
  • Digital/Print
    Robert B. Denman, editor.
    Digital : Springer2012
    Print2012
    Introduction: Reminiscing on Models and Modeling / Robert B. Denman -- Probing Astrocyte Function in Fragile X Syndrome / Shelley Jacobs, Connie Cheng and Laurie C. Doering -- Neural Stem Cells / Maija Castrén -- Fragile X Mental Retardation Protein (FMRP) and the Spinal Sensory System / Theodore J. Price and Ohannes K. Melemedjian -- The Role of the Postsynaptic Density in the Pathology of the Fragile X Syndrome / Stefan Kindler and Hans-Jürgen Kreienkamp -- Behavior in a Drosophila Model of Fragile X / Sean M. McBride, Aaron J. Bell and Thomas A. Jongens -- Molecular and Genetic Analysis of the Drosophila Model of Fragile X Syndrome / Charles R. Tessier and Kendal Broadie -- Fragile X Mental Retardation Protein and Stem Cells / Abrar Qurashi, Xuekun Li and Peng Jin -- Manipulating the Fragile X Mental Retardation Proteins in the Frog / Marc-Etienne Huot, Nicolas Bisson, Thomas Moss and Edouard W. Khandjian -- Exploring the Zebra Finch Taeniopygia guttata as a Novel Animal Model for the Speech-Language Deficit of Fragile X Syndrome / Claudia Winograd and Stephanie Ceman -- Neuroendocrine Alterations in the Fragile X Mouse / Abdeslem El Idrissi, Xin Yan, William L'Amoreaux, W. Ted Brown and Carl Dobkin -- Taking STEPs Forward to Understand Fragile X Syndrome / Susan M. Goebel-Goody and Paul J. Lombroso -- Fmr-1 as an Offspring Genetic and a Maternal Environmental Factor in Neurodevelopmental Disease / Bojana Zupan and Miklos Toth -- Mouse Models of the Fragile X Premutation and the Fragile X Associated Tremor/Ataxia Syndrome / Michael R. Hunsaker, Gloria Arque, Robert F. Berman, Rob Willemsen and Renate K. Hukema -- Clinical Aspects of the Fragile X Syndrome / W. Ted Brown -- Fragile X Syndrome: A Psychiatric Perspective / Michael R. Tranfaglia -- Fragile X Syndrome and Targeted Treatment Trials / Randi Hagerman, Julie Lauterborn, Jacky Au and Elizabeth Berry-Kravis -- The Fragile X-Associated Tremor Ataxia Syndrome / Flora Tassone and Randi Hagerman -- Vignettes: Models in Absentia / Robert B. Denman.
  • Digital
    María Elena Álvarez-Buylla Roces, Juan Carlos Martínez-García, José Dávila-Velderrain, Elisa Domínguez-Hüttinger, Mariana Esther Martínez-Sánchez.
    Springer2018
    "This book contributes to better understand how lifestyle modulations can effectively halt the emergence and progression of human diseases. The book will allow the reader to gain a better understanding of the mechanisms by which the environment interferes with the bio-molecular regulatory processes underlying the emergence and progression of complex diseases, such as cancer. Focusing on key and early cellular bio-molecular events giving rise to the emergence of degenerative chronic disease, it builds on previous experience on the development of multi-cellular organisms, to propose a mathematical and computer based framework that allows the reader to analyze the complex interplay between bio-molecular processes and the (micro)-environment from an integrative, mechanistic, quantitative and dynamical perspective. Taking the wealth of empirical evidence that exists it will show how to build and analyze models of core regulatory networks involved in the emergence and progression of chronic degenerative diseases, using a bottom-up approach"--Publisher's description.
  • Digital
    volume editors, Marilyn M. Bui, Liron Pantanowitz.
    Karger2020
    "The purpose of this book is to describe, illustrate, and review many of the most recent developments regarding modern techniques employed in cytopathology. It is intended for all cytologists, including cytopathologists, cytotechnologists, cytology lab assistants, trainees, research scientists, and anyone who is interested in the field of cytopathology"-- Provided by publisher.
  • Digital
    Mark Ping Chao.
    Growing evidence indicates that cancers are composed of functionally heterogeneous cells that are organized by a cellular hierarchy and are maintained by a stem cell-like population. Given their unique ability to initiate tumor growth, eradication of these cancer stem cells (CSC) is necessary for cure. Thus, the development of CSC targeted therapies is necessary to improve current clinical outcomes. This body of work identifies CD47 as a therapeutic target on CSC and bulk cells in several human malignancies. CD47 is a cell surface protein that functions to inhibit phagocytosis through binding its ligand SIRP[Alpha], expressed on phagocytes. Compared to normal cell counterparts, CD47 was highly expressed on several human tumors including acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), and non-Hodgkin lymphoma (NHL), suggesting that higher CD47 levels on tumors allow evasion of immune phagocytosis. Higher CD47 expression in these tumors correlated with a worse clinical prognosis and was an independent prognostic factor. Furthermore, a monoclonal blocking antibody against CD47 enabled phagocytosis of human AML, ALL, and NHL in vitro and eliminated disease in vivo. When combined with the Fc receptor-activating antibody, rituximab, anti-CD47 antibody therapy achieved high cure rates in pre-clinical models of NHL in contrast to partial responses with either agent alone. Lastly, anti-CD47 antibody treatment specifically targeted tumor but not normal cells, resulting in minimal toxicity in vitro and in vivo. The selective targeting of tumor cells by anti-CD47 antibody was due to the selective expression of the pro-phagocytic signal, calreticulin on tumor but not normal cells. In summary, this body of work identifies CD47 as a therapeutic target on several human cancers and that a monoclonal blocking antibody against CD47 has pre-clinical efficacy in eliminating these tumors through the promotion of phagocytosis.
  • Digital
    edited by Michael S. Wolfe.
    ScienceDirect2018
    The Molecular and Cellular Basis of Neurodegenerative Diseases: Underlying Mechanisms presents the pathology, genetics, biochemistry and cell biology of the major human neurodegenerative diseases, including Alzheimer's, Parkinson's, frontotemporal dementia, ALS, Huntington's, and prion diseases. Edited and authored by internationally recognized leaders in the field, the book's chapters explore their pathogenic commonalities and differences, also including discussions of animal models and prospects for therapeutics. Diseases are presented first, with common mechanisms later. Individual chapters discuss each major neurodegenerative disease, integrating this information to offer multiple molecular and cellular mechanisms that diseases may have in common. This book provides readers with a timely update on this rapidly advancing area of investigation, presenting an invaluable resource for researchers in the field.
  • Digital
    Maurice B. Hallett editor.
    Springer2020
    Intro -- Preface -- Contents -- Contributors -- 1 An Introduction to Phagocytosis -- Introduction to Phagocytosis -- The Cell Biology of Phagocytosis -- Phagocytosis Dissected -- The Importance of Phagocytosis -- Evolution and Phagocytosis -- Introduction to the Chapters in This Book -- 2 A Brief History of Phagocytosis -- Introduction -- Purpose of Chapter -- Let's Start at the Very Beginning -- The Eureka Moment of Metchnikoff -- Inside the Phagocyte -- Phagosomal pH -- Ca2+ Ions and Phagocytosis -- Conclusion -- 3 The Role of Membrane Surface Charge in Phagocytosis -- Introduction Lipids That Contribute to Phagocytosis and Phagosome Maturation -- Phospholipid Dynamics and Inner Leaflet Charge During Phagocytosis and Phagosome Maturation -- Role of Electrostatic Interactions in Protein Localization During Phagocytosis and Phagosome Maturation -- Relevance of Inner Leaflet Surface Charge to Phagocytes and Immunity -- Concluding Remarks -- 4 Receptor Models of Phagocytosis: The Effect of Target Shape -- Introduction -- Why Modelling? -- Receptor Models of Phagocytosis -- The Basic Receptor Model -- Phagocytosis Versus Other Types of Endocytosis -- The Effect of Target Shape Known Experimental Results -- Modelling Shape Dependence -- Model Extensions and the Future -- Summary -- 5 Decision Making in Phagocytosis -- Introduction -- Uptake, Processing, and Release of Long Particles -- Uptake of a Long Particle -- Phagosome Processing -- Release of a Particle -- Myosin-II Does Not Accumulate on the Incipient Phagosome -- Conversion of a Tubular Phagosome into a Bleb -- Simultaneous Uptake and Release of Particles -- Struggle for a Bacterium -- Discussion -- 6 Membrane Tension and the Role of Ezrin During Phagocytosis -- Introduction Apparent Expansion of Cell Surface Area During Phagocytosis -- Exocytotic Addition to the Plasma Membrane -- Cell Surface "Wrinkles" -- Membrane Tension and Cell Shape Changes -- Wrinkles and Membrane Tension -- Molecular Maintenance of Membrane Tension -- Ezrin -- Ezrin and Membrane Tension -- Ezrin and Phagocytosis -- Regulation Membrane Tension by Ezrin During Phagocytosis -- Actin Push and the Brownian Ratchet -- Conclusion and Synthesis -- 7 Molecular Mechanisms of Calcium Signaling During Phagocytosis -- Abbreviations -- Introduction -- Overview of Ca2+ Signaling -- Phagocytic Ca2+ Signals Ca2+-Permeable Channels Involved in Phagocytic Ca2+ Signals -- ORAI Channels, STIM Proteins and Junctate -- TRPC Channels -- TRPC3 -- TRPC4/5 -- TRPC6 -- TRPM Channels -- TRPM2 -- TRPM8 -- TRPML Channels -- TRPV Channels -- TRPV1 and TRPV2 -- TRPV4 -- P2X Channels -- P2X7 -- P2X4 -- Conclusions and Future Directions -- 8 Calpain Activation by Ca2+ and Its Role in Phagocytosis -- Introduction -- Ca2+ in Phagocytosis and Cell Shape Change -- Calpain Activation in Shape Change -- Calpain in Phagocytosis -- Structure of Calpain 1 and 2 -- Physiological Activation of Calpain by Ca2+ in Cells Conclusions
  • Digital
    Fernando C. Schmitt, editor.
    Springer2018
    This book is intended for practicing pathologists and cytopathologists, as well as for pathology trainees and cytotechnicians. It starts with a detailed description of the extremely important pre-analytical phase for molecular testing followed by a presentation of the key tests and their application in different organs, e.g. the lung or thyroid. Step-by-step instructions for the different assays, reporting and clinical integration of the test results are discussed. The authors help the reader to benefit from their experiences by providing a valuable tool for the implementation of these techniques in daily practice. Though the use of molecular techniques is well established in surgical biopsies, to date they are not widely used in connection with cytological material. However, in some fields like lung cancer or aspirates from the pancreas and biliary tract the only available material for diagnosis is the cytological preparation a fact that has created a need for the standardization of molecular techniques on cytology. .
  • Digital
    Amittha Wickrema, Barbara Kee, editors.
    Springer2009
  • Digital/Print
    Roland Martin, Andreas Lutterotti (eds.).
    Digital : Springer2010
    Print2010
  • Digital/Print
    Bruce Alberts ... [et al.].
    Digital : NCBI Bookshelf2002Fulltext accessible by its search box
    Print2002
    Print2002
  • Print
    Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter ; with problems by John Wilson, Tim Hunt.
    Status: Not Checked Out
    "As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts. As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced. Protein structures better illustrate structure-function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing open-ended questions highlighting "What We Don't Know," introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text. Thought-provoking end-of-chapter questions have been expanded to all chapters, including questions on developmental biology, tissues and stem cells, the immune system, and pathogens."-- Provided by publisher.
  • Digital/Print
    Harvey Lodish ... [et al.].
    Digital : NCBI Bookshelf2000
    Print2000
  • Print
    Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott.
    Status: Not Checked Out
    Overview: Molecular Cell Biology presents the key concepts in cell biology and their experimental underpinnings. The authors, all world-class researchers and teachers, incorporate medically relevant examples where appropriate to help illustrate the connections between cell biology and health and human disease. As always, a hallmark of MCB is the use of experiments to engage students in the history of cell biology and the research that has contributed to the field. The book contains predominantly color illustrations, with some black-and-white illustrations.
  • Print
    Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Kelsey C. Martin.
    Status: Not Checked Out
    Pt. I, Chemical and molecular foundations : -- Molecules, cells, and model organisms -- Chemical foundations -- Protein structure and function -- Culturing and visualizing cells. Pt. II, Biomembranes, genes, and gene regulation : -- Fundamental molecular genetic mechanisms -- Molecular genetic techniques -- Biomembrane structure -- Genes, genomics, and chromosomes -- Transcriptional control of gene expression -- Post-transcriptional gene control. Pt. III, Cellular organization and function : -- Transmembrane transport of ions and small molecules -- Cellular energetics -- Moving proteins into membranes and organelles -- Vesicular traffic, secretion, and endocytosis -- Signal transduction and G protein, coupled receptors -- Signaling pathways that control gene expression -- Cell organization and movement I: microfilaments -- Cell organization and movement II: microtubules and intermediate filaments -- The Eukaryotic cell cycle. Pt. IV, Cell growth and differentiation : -- Integrating cells into tissue -- Stem cells, cell asymmetry, and cell death -- Cells of the nervous system -- Immunology -- Cancer.
  • Digital
    edited by Lorenzo Galluzzi, José Manuel Bravo-San Pedro and Guido Kroemer.
    ScienceDirectPt. A
    ScienceDirectPt. B
  • Digital
    Bin Yang, Jianyu Rao, editors.
    Springer2016
    1. Introduction -- 2. Advantage of molecular testing using cytologic materials -- 3. Validation of molecular techniques in liquid-based specimens -- 4. Establishment of molecular cytology laboratory -- 5. Researches on molecular cytopathology -- 6. Molecular Biomarkers in cervical cytology -- 7. Molecular Biomarkers in thyroid cytology -- 8. Molecular Biomarkers in head and neck cytology -- 9. Molecular Biomarkers in pulmonary cytology -- 10. Molecular Biomarkers in breast cytology -- 11. Molecular Biomarkers in urine cytology -- 12. Molecular Biomarkers in hepatobiliary and pancreatic cytology -- 13. Molecular Biomarkers in mesothelioma and body fluid cytology -- 14. Molecular Biomarkers in soft tissue cytology -- 15. Molecular Biomarkers in brain tumors -- 16. Molecular cytopathology in therapeutic surveillance.
  • Digital/Print
    edited by Michael M. Gottesman.
    Digital : ScienceDirect1987
    Print1987
  • Print
    S.S. Gambhir.
    Status: Not Checked Out
    Chapter 1. Molecular imaging -- Chapter 2. Basic cell & molecular biology -- Chapter 3. Ovreview of imaging modalities -- Chapter. 4. Molecular imaging strategies -- Chapter 5. Assays -- Chapter 6. Nanoparticle imaging -- Chapter 7. Clinical applications -- Chapter 8. Molecular imaging examples -- Chapter 10. Frequently asked questions -- Chapter 11. Useful tables -- Chapter 12. Probe structures table.
  • Digital
    volume editor, Karl Lenhard Rudolph.
    Karger2010
    Speakers at the Symposium / Hartmann, D. -- Hematopoietic stem cell aging and fate decision / Illing, A., Ju, Z. -- Alterations of systemic environment impair stem cell function during aging / Song, Z., Ju, Z. -- Novel insights into regulation of stem cell aging by aging of the niche and alterations in systemic factors / Geiger, H. -- Aging and hematopoietic malignancies / Gompf, A. -- Intestinal stem cell aging / Sperka, T., Rudolph, K.L. -- Molecular mechanisms of aging in muscle stem cells / Tümpel, S. -- Free radicals and stem cell aging / Guachalla, L.M. -- DNA damage, checkpoint responses, and cell cycle control in aging stem cells / Sperka, T., Nalapareddy, K., Rudolph, K.L.
  • Digital/Print
    Rafal P. Piprek, editor.
    Digital : Springer2016
    Print2016
    Early Development of the Gonads -- Origin and Differentiation of the Somatic Cells of the Genital Ridges -- The formation and migration of primordial germ cells in mouse and man -- The Gonadal Supporting Cell Lineage and Mammalian Sex Determination: the Differentiation of Sertoli and Granulosa Cells -- Mesonephric cell migration into the gonads and vascularization are processes crucial for testis development -- Origin and Differentiation of Androgen-producing Cells in the Gonads -- Germ cell commitment to oogenic versus spermatogenic pathway -- the role of retinoic acid -- Ovarian Folliculogenesis -- Control of oocyte growth and development by intercellular communication within the follicular niche -- Biology of the Sertoli cell in the Fetal, Pubertal and Adult Mammalian Testis -- Mechanisms Regulating Spermatogonial Differentiation -- Stem cells in mammalian gonads -- A Role of microRNAs in Cell Differentiation during Gonad Development -- The Battle of the Sexes: Human Sex Development and its Disorders -- Methods for the study of gonadal development.
  • Digital
    Benjamin Bonavida, editor.
    Springer2013
    Patients with various cancers are treated with conventional chemotherapeutic drugs and the majority responds well to such therapies. However, there is a subset of patients who does not respond initially and another subset who no longer responds to further treatments. Clearly, in those two subsets of patients, the cancer cells exhibit mechanisms of resistance. One of the main challenges facing us to date is to develop new therapies to treat patients with the resistant tumors. The development of new effective therapies will be dependent on delineating the biochemical, molecular, and genetic mechanisms that regulate tumor cell resistance. Such mechanisms have revealed gene products that directly regulate resistance and are targets for therapy. Of interest, several FDA-approved drugs were able to overcome drug resistance and have been successfully used clinically. They have been used as monotherapy or synergized with other therapies for the treatment of resistant tumors. This volume constitutes a total of twelve selective reviews by pioneer scientists in the field of cancer drug resistance. Specific mechanisms in drug resistance are reviewed and novel approaches are being proposed for therapeutic interventions. This volume is of general interest to scientists, clinicians, health care providers, and students.
  • Digital
    edited by Christophe Lavelle.
    Springer2018
  • Digital
    Abdulrasheed A. Alabi.
    Regulated exo-endocytosis is essential for the function of many intercellular signaling networks. Using a new optical nanosensor termed pHdot, I explore the molecular and cellular signals that tune the balance between two modes of vesicle fusion and recycling: kiss-and-run (KR) and full collapse fusion (FF) in hippocampal synapses in primary culture. Stimulation after a period of rest generates a frank initial rise in pHdot fluorescence, an unmistakeable signature of early phase KR. The prevalence of KR was dependent on the available pool of readily releasable vesicles and modulated by internal Ca2+ dynamics. Intriguingly, pharmacological inhibition of the Ca2+-dependent phosphatase calcineurin and stimulation of synapses with phorbol esters had dramatic yet opposing effects on fusion mode balance; providing a means of turning KR "on" or "off" respectively. Emerging data further suggests calcineurin facilitates a molecular switch from KR to FF that is dependent on actin dynamics and the F-BAR domain-containing protein syndapin I. Altogether, this body of work provides mechanistic insight into what appears to be a rather complex molecular choreography underlying kiss-and-run fusion at small, central synapses. The rich set of modulatory effects described could conceivably account for disparate reports on the prevalence of kiss-and-run at nerve terminals, a source of longstanding controversy. More exciting however is the likelihood that these findings reflect the diversity of signals and proteins that participate in shaping the energetics of fusion pore formation thus providing a glimpse into the intricate biochemical milieu that a vesicle navigates to direct its dynamic release properties.
  • Digital
    Emily Lawson Egeler.
    Cellular maintenance of protein homeostasis , or proper protein folding, is essential for normal cellular function. Accumulation of misfolded protein can lead to disease and even cell death. The ubiquitin-proteasome system plays a central role in processing cellular proteins for degradation, but little is currently known about how misfolded cytosolic proteins are recognized by protein quality control machinery and targeted for degradation in mammalian cells. In the work presented here, we work with a model protein substrate that is unstable when expressed in cells, but stabilized by addition of a small cell-permeable ligand. Using purified protein, we show that unstable mutants are poorly folded or rapidly sampling unfolded states. The propensity to unfold in vitro correlates with observed instability when expressed in cells. We also show that these substrates are rapidly ubiquitinated en route to degradation at the proteasome in a ligand-dependent manner. We conducted a mass spectrometry screen to identify endogenous protein interactors with the model substrate, which identified a few annotated ubiquitin-proteasome system components. The findings presented herein correlate observed cellular instability to both the propensity of the protein to unfold and the extent of ubiquitination in the ligand-free state. We propose that this protein is a ligand-dependent substrate for the protein quality control machinery, providing an excellent substrate into further query the mechanisms of protein quality control.
  • Digital
    Domenico Ribatti.
    Springer2012
    Introduction -- Morphological aspects of tumor vasculature -- Mechanisms of control of angiogenesis in tumor vasculature -- Anti-Angiogenesis and vascular targeting in tumor vasculature.
  • Digital
    Lorenzo Alibardi.
    Springer2010
  • Digital
    editor, Gerhard Seifer ; contributors, M. Altmannsberger and others.
    Springer1987
    New methods in immunocytochemistry and hybridization techniques enable the pathologist active in diagnosis to clarify more effectively problems in the classification and prognosis of tumors. By adopting these methods into his diagnostic repertoire it will be possible to create a closer, more productive connection between morphological diagnosis and clinical work. This volume gives the reader an up-to-date general survey from international experts of the method, technique and practical application of these new procedures.
  • Digital
    Giuseppe d'Onofrio, Gina Zini ; translated by Barbara J. Bain.
    Wiley2015
    Blood and bone marrow cells -- Erythropoiesis and anaemia -- Myelodysplastic syndromes -- Acute myeloid leukaemia -- Myeloproliferative neoplasms -- Hypereosinophilia -- Myelodysplastic/myeloproliferative neoplasms -- Acute lymphoblastic and mixed phenotype leukaemia -- Neoplasms of mature B, T and NK cells -- Plasma cell proliferative disorders -- Reactive bone marrow changes and non-haemopoietic neoplasms.
  • Digital
    Sunil C. Kaul, Renu Wadhwa, editors.
    Springer2012
    Part 1. Structure and Function of Mortalin / Birth of Mortalin: Multiple Names, Niches and Functions Connecting Stress, Senescence and Cancer / Renu Wadhwa and Sunil C. Kaul -- Mortalin's Machinery / Custer C. Deocaris, Sunil C. Kaul and Renu Wadhwa -- The Role of Mortalin in Iron Homeostasis / Wen-I Luo and James A. Cowan -- Functional Characteristics of Mortalin / Walter A. Baseler, Tara L. Croston and John M. Hollander -- Part 2. Mortalin in Evolution2 Deocaris / Mortalin and Drosophila DmHsp22: Two Mitochondrial Chaperones Regulating Aging and Carcinogenesis / Marie Le Pécheur, Geneviève Morrow and Robert M. Tanguay -- Mortalin in Invertebrates and The Induction of Apoptosis by Wild-Type p53 Following Defeat of Mortalin-Based Cytoplasmic Sequestration in Cancerous Clam Hemocytes / Charles W. Walker, Ben Low and S. Anne Böttger -- Mortalin and Stem Cells: A Study from Planarians / Renata Batistoni -- Part 3. Mortalin in Health and Disease / Mortalin in Cell Protection from Immune Attack / Moran Saar, Oren Moskovich and Zvi Fishelson -- Mortalin in Neurological Diseases / Jinghua Jin, Jing Zhang, Travis J. Cook and Jake G. Hoekstra -- Loss of Mortalin Function in Parkinson's Disease-Supporting the Mitochondrial Pathway of Neurodegeneration / Lena F. Burbulla and Rejko Krüger -- Hsp75/mortalin and Protection from Ischemic Brain Injury / Robin E. White, Yi-Bing Ouyang and Rona G. Giffard -- Catecholamine Regulated Protein (CRP40), A Splice Variant of Mortalin-2: Functional Role in CNS Disorders / Joseph P. Gabriele, Sarah E. Groleau, Ritesh P. Daya, Zdenek B. Pristupa and Ram K. Mishra -- Chaperonopathies: Diseases in Which Mortalin and Other Hsp-Chaperones Play a Role in Etiology and Pathogenesis / Alberto J. L. Macario, Francesco Cappello and Everly Conway de Macario -- Part 4. Mortalin and Cancer / Many Faces of Mortalin and Tid1 / Ohad Iosefson and Abdussalam Azem -- Mortalin: A Positive Regulator of Centrosome Duplication and Amplification / Masayuki Kanai and Kenji Fukasawa -- Mortalin Expression in Normal and Neoplastic Tissues / Angheliki Nomikos, Sinclair R. Dundas and Graeme I. Murray -- Mortalin-p53 Interaction as a Target for Liver Cancer Therapy / Wen-Jing Lu, Nikki P. Lee, Renu Wadhwa and John M. Luk -- Mortalin Targeting Gadgets for Cancer Therapy / Chae-Ok Yun and Renu Wadhwa -- Part 5. Mortalin Based Technologies / Cell Internalizing Anti-Mortalin Antibody for Generation of Illuminating MSCs for Long-Term In vitro and In vivo Tracking / Toshimasa Uemura, Masanori Nishi, Sunil C. Kaul and Renu Wadhwa -- Mortalin Staining Pattern as a Reporter for Cell Based Anti-Cancer Drug Screening / Ran Gao, Zeenia Kaul, Tomoko Yaguchi and Renu Wadhwa -- Cell Internalizing Anti-mortalin Antibody as a Nanocarrier / Zeenia Kaul, Tomoko Yaguchi, Renu Wadhwa and Sunil C. Kaul.
  • Digital/Print
    Jacek Z. Kubiak, editor.
    Digital : Springer2012
    Print2012
    Post-transcriptional Control of Gene Expression During Mouse Oogenesis / Hugh J. Clarke -- Polarity and Asymmetry During Mouse Oogenesis and Oocyte Maturation / Malgorzata Kloc, Rafik M. Ghobrial, Ewa Borsuk and Jacek Z. Kubiak -- Chromatin Structure and ATRX Function in Mouse Oocytes / Rabindranath De La Fuente, Claudia Baumann and Maria M. Viveiros -- Cyclin B in Mouse Oocytes and Embryos: Importance for Human Reproduction and Aneuploidy / Zbigniew Polański, Hayden Homer and Jacek Z. Kubiak -- Src Protein Kinases in Mouse and Rat Oocytes and Embryos / Mattan Levi, Lihi Ninio-Mani and Ruth Shalgi -- Gradual Meiosis-To-Mitosis Transition in the Early Mouse Embryo / Aurélien Courtois and Takashi Hiiragi -- Maternal Control of Mouse Preimplantation Development / Wenjing Zheng and Kui Liu -- Preimplantation Mouse Embryo: Developmental Fate and Potency of Blastomeres / Aneta Suwińska -- Creation of Trophectoderm, the First Epithelium, in Mouse Preimplantation Development / Yusuke Marikawa and Vernadeth B. Alarcon -- Cell Lineage Allocation Within the Inner Cell Mass of the Mouse Blastocyst / Panagiotis Xenopoulos, Minjung Kang and Anna-Katerina Hadjantonakis -- Formation of Distinct Cell Types in the Mouse Blastocyst / Samantha A. Morris and Magdalena Zernicka-Goetz -- Cell Movements in the Egg Cylinder Stage Mouse Embryo / Bradley Joyce and Shankar Srinivas -- Balancing the Dose in the Mouse / Mary E. Donohoe -- Mouse Oviduct Development / C. Allison Stewart and Richard R. Behringer -- Cell Lineages, Growth and Repair of the Mouse Heart / Fabienne Lescroart and Sigolène M. Meilhac -- Cellular Reprogramming During Mouse Development / Zoë D. Burke, Gabriela Miron-Buchacra and David Tosh -- Differentiation of Definitive Endoderm from Mouse Embryonic Stem Cells / Peter T. W. Kim and Christopher J. Ong -- Mouse and Human Pluripotent Stem Cells and the Means of Their Myogenic Differentiation / I. Grabowska, K. Archacka, A. M. Czerwinska, M. Krupa and M. A. Ciemerych -- Stem Cells and Corneal Epithelial Maintenance: Insights from the Mouse and Other Animal Models / Richard L. Mort, Panagiotis Douvaras, Steven D. Morley, Natalie Dorà and Robert E. Hill, et al. -- Mouse-Induced Pluripotent Stem Cells / Hui Zhang and Ying Jin -- Aging in the Mouse and Perspectives of Rejuvenation Through Induced Pluripotent Stem Cells (iPSCs) / Ken-ichi Isobe, Zhao Cheng, Sachiko Ito and Naomi Nishio.
  • Digital
    edited by Mark Lewandoski.
    Springer2014
    In situ Hybridization Methods for Mouse Whole Mounts and Tissue Sections with and Without Additional [beta]-galactosidase Staining -- Two Color In Situ Hybridization of Whole-Mount Mouse Embryos -- Detection and Monitoring of MicroRNA Expression in Developing Mouse Brain and Fixed Brain Cryosections -- Laser Capture Microdissection of Embryonic Cells and Preparation of RNA for Microarray Assays -- EMAGE: Electronic Mouse Atlas of Gene Expression -- Real-time PCR Quantification of gene Expression in Embryonic Mouse Tissue -- Identifying Essential Genes in Mouse Development Via an ENU-based Forward Genetic Approach -- Generation of Mouse Embryos with Small Hairpin RNA-mediated Knockdown of Gene Expression -- Generation of Tissue Organoids by Compaction Reaggregation -- Ultra-Rapid Vitrification of Mouse Oocytes and Embryos -- Mammalian Preimplantation Embryo Culture -- Serum-Free Culture of Mid-Gestation Mouse Embryos: A Tool for the Study of Endoderm-Derived Organs -- Genetically Encoded Probes Provide a Window On Embryonic Arrhythmia -- Microscopic Computed Tomography-based Skeletal Phenotyping for Genetic Model Organisms -- Gene Transfer Techniques in Whole Embryo Cultured Post-Implantation Mouse Embryos -- Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues -- Protein/Peptide Transduction in Metanephric Explant Culture -- Direction of Cells Programmed to Die in Mouse Embroys -- Microscopic Computed Tomography-based ?Virtual Histology of Embryos -- Collection and Preparation of Rodent Embryonic Samples for Transcriptome Study -- The Latest Improvements in the Mouse Sperm Preservation -- Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ in vitro -- Using the Textpresso Site Specific Recombinases Web Server to Identify ?Cre Expressing Mouse Strains and Floxed Alleles -- Live Imaging Mouse Embryonic Development: Seeing is Believing and Revealing -- Genetic Cell Ablation -- Essentials of Recombinase-based Genetic Fate Mapping in Mice.
  • Digital
    edited by Marie-Hélène Verlhac, Marie-Emilie Terret.
    Springer2018
  • Digital
    Tim Chard, Richard Lilford.
    Springer1987
    MRCOG Part 1 is a multiple choice question and answer volume dealing with the basic sciences in obstetrics and gynaecology. The overall aim of the volume is to give candidates for examinations such as the MCROG Part 1 as close as possible a flavour of the examination itself. The material presented in the book covers cell biology, embryology and the placenta, anatomy, general physiology and biochemistry, endocrinology, pathology, microbiology, pharmacology, statistics, and ultrasound. For those who seek information in addition to the answer provided, a reference to the appropriate page number in the textbook "Basic Sciences in Obstetrics and Gynaecology, 2nd edition" by Professors Chard and Lilford is also included in the answer.
  • Digital
    edited by Thomas Weichhart.
    Springer2012
    Mammalian target of rapamycin : a signaling kinase for every aspect of cellular life / Thomas Weichhart -- Biochemical and pharmacological inhibition of mTOR by rapamycin and an ATP-competitive mTOR inhibitor / Ker Yu and Lourdes Toral-Barza -- Evaluation of the nutrient-sensing mTOR pathway / Sungki Hong, Aristotle M. Mannan, and Ken Inoki -- mTor activity under hypoxia / Douangsone D. Vadysirisack and Leif W. Ellisen -- Isolation of the mTOR complexes by affinity purification / Dos D. Sarbassov [and others] -- An in vitro assay for the kinase activity of mTOR complex 2 / Jingxiang Huang -- Overexpression or downregulation of mTOR in mammalian cells / Mahmoud Khalil and Ivan Gout -- Detection of cytoplasmic and nuclear functions of mTOR by fractionation / Margit Rosner and Markus Hengstschläger -- Evaluation of rapamycin-induced cell death / Lorenzo Galluzzi [and others] -- Evaluation of mTOR-regulated mRNA translation / Valentina Iadevaia [and others] -- A genome-wide RNAi screen for polypeptides that alter rpS6 phosphorylation / Angela Papageorgiou and Joseph Avruch -- Immunohistochemical analysis of mTOR activity in tissues / Jinhee Kim [and others] -- Assessing cell size and cell cycle regulation in cells with altered TOR activity / Megan Cully and Julian Downward -- Quantitative visualization of autophagy induction by mTOR inhibitors / Beat Nyfeler [and others] -- The in vivo evaluation of active-site TOR inhibitors in models of BCR-ABL+ leukemia / Matthew R. Janes and David A. Fruman -- Inducible raptor and rictor knockout mouse embryonic fibroblasts / Nadine Cybulski, Vittoria Zinzalla, and Michael N. Hall -- Expanding human T regulatory cells with the mTOR-inhibitor rapamycin / Manuela Battaglia, Angela Stabilini, and Eleonora Tresoldi -- Rapamycin-induced enhancement of vaccine efficacy in mice / Chinnaswamy Jagannath and Pearl Bakhru -- Utilizing a retroviral RNAi system to investigate in vivo mTOR functions in T cells / Koichi Araki and Bogumila T. Konieczny -- Exploring functional in vivo consequences of the selective genetic ablation of mTOR signaling in T helper lymphocytes / Greg M. Delgoffe and Jonathan D. Powell -- Evaluating the therapeutic potential of mTOR inhibitors using mouse genetics / Huawei Li, Jennifer L. Cotton, and David A. Guertin -- Inhibition of PI3K-Akt-mTOR signaling in glioblastoma by mTORC1/2 inhibitors / Qi-Wen Fan and William A. Weiss -- Assessing the function of mTOR in human embryonic stem cells / Jiaxi Zhou, Dong Li, and Fei Wang -- Video-EEG monitoring methods for characterizing rodent models of tuberous sclerosis and epilepsy / Nicholas R. Rensing, Dongjun Guo, and Michael Wong -- A genetic model to dissect the role of TSC-mTORC1 in neuronal cultures / Duyu Nie and Mustafa Sahin -- Tissue-specific ablation of TSC1 in pancreatic beta-cells / Hiroyuki Mori and Kun-Liang Guan -- A mouse model of diet-induced obesity and insulin resistance / Chao-Yung Wang and James K. Liao -- Rapamycin as immunosuppressant in murine transplantation model / Louis-Marie Charbonnier and Alain Le Moine -- Development of ATP-competitive mTOR inhibitors / Qingsong Liu [and others].
  • Digital
    Ruslan I. Dmitriev, editor.
    Springer2017
    Current state-of-the-art 3D tissue models and their compatibility with live cell imaging -- Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation -- Quantitative live cell FLIM imaging in three dimensions -- Three-dimensional tissue models and available probes for multi-parametric live cell microscopy: a brief overview -- Fabrication and handling of 3D scaffolds based on polymers and decellularized tissues -- Multi-parametric imaging of hypoxia and cell cycle in intestinal organoid culture -- Imaging of intracellular pH in tumor spheroids using genetically encoded sensor SypHer2 -- Application of fluorescence lifetime imaging (FLIM) to measure intracellular environments in a single cell -- Quantitative imaging of Ca2+ by 3D-FLIM in live tissues -- Live cell imaging of viscosity in 3D tumour cell models -- Live imaging of cell invasion using a multicellular spheroid model and lightsheet microscopy -- Raman imaging microscopy for quantitative analysis of biological samples.
  • Digital
    edited by Anna Porwit, Marie Christine Béné.
    Cambridge2018
    Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.
  • Digital
    Meletios A. Dimopoulos, Thierry Facon, Evangelos Terpos, editors.
    Springer2018
  • Digital
    Allison D. Fryer, Arthur Christopoulos, Neil M. Nathanson, editors.
    Springer2012
    Muscarinic receptor pharmacology and signaling -- Overview of muscarinic receptor subtypes -- Structure-function studies of muscarinic acetylcholine receptors -- Polymorphisms in human muscarinic receptor subtype genes -- Muscarinic receptor trafficking -- Physiological role of G-protein coupled receptor phosphorylation -- Novel muscarinic receptor mutant mouse models -- Muscarinic receptors in the CNS -- Muscarinic receptor pharmacology and circuitry for the modulation of cognition -- Muscarinic agonists and antagonists in schizophrenia -- Muscarinic pain pharmacology: realizing the promise of novel anagesics by overcoming old challenges -- Muscarinic modulation of striatal function and circuitry -- Muscarinic receptors in brain stem and mesopontine cholinergic arousal functions -- Muscarinic receptors in autonomic effector organs -- Muscarinic receptor agonists and antagonists: effects on ocular function -- Muscarinic receptor agonists and antagonists: effects on cardiovascular function -- Muscarinic receptor antagonists: effects on pulmonary function -- Muscarinic agonists and antagonists: effects on gastrointestinal function -- Muscarinic agonists and antagonists: effets on the urinary bladder -- Muscarinic receptors and mediation of hormonal effects of acetylcholine -- Muscarinic receptor agonists and antagonists: effects on inflammation and immunity -- Muscarinic receptor agonists and antagonists: effects on keratinocyte functions -- Muscarinic receptor agonists and antagonists: effects on cancer -- Activation of muscarinic receptors by non-neuronal acetylcholine.
  • Digital
    edited by Eusebio Perdiguero, DDW Cornelison.
    Springer2017
    Muscle stem cells : a model system for adult stem cell biology / DDW Cornelison and Eusebio Perdiguero -- Isolation of muscle stem cells from mouse skeletal muscle / Barbara Gayraud-Morel, Francesca Pala, Hiroshi Sakai, and Shahragim Tajbakhsh -- Primary mouse myoblast purification using magnetic cell separation / Marie Claude Sincennes, Yu Xin Wang, and Michael A. Rudnicki -- Isolation, culture, and immunostaining of skeletal muscle myofibers from WildType and Nestin-GFP mice as a means to analyze satellite cells / Pascal Stuelsatz, Paul Keire, and Zipora Yablonka-Reuveni -- Characterization of drosophila muscle stem cell-like adult muscle precursors / Guillaume Lavergne, Cedric Soler, Monika Zmojdzian, and Krzysztof Jagla -- Using transgenic zebrafish to study muscle stem/progenitor cells / Phong D. Nguyen and Peter D. Currie -- Muscle interstitial cells : a brief field guide to non-satellite cell populations in skeletal muscle / Francesco Saverio Tedesco, Louise A. Moyle, and Eusebio Perdiguero -- Isolation and characterization of vessel-associated stem/progenitor cells from skeletal muscle / Rossana Tonlorenzi, Giuliana Rossi, and Graziella Messina -- Fibro/adipogenic progenitors (FAPs) : Isolation by FACS and culture / Marcela Low, Christine Eisner, and Fabio Rossi -- Single cell gene expression profiling of skeletal muscle-derived cells / Sole Gatto, Pier Lorenzo Puri, and Barbora Malecova -- Engraftment of FACS isolated muscle stem cells into injured skeletal muscle / Matthew Tierney and Alessandra Sacco -- Transplantation of skeletal muscle stem cells / Monica N. Hall, John K. Hall, Adam B. Cadwallader, Bradley T. Pawlikowski, Jason D. Doles, Tiffany L. Elston, and Bradley B. Olwin -- Simultaneous measurement of mitochondrial and glycolytic activity in quiescent muscle stem cells / James G. Ryall -- Monitoring autophagy in muscle stem cells / Laura García-Prat, Pura Muñoz-Cánoves, and Marta Martínez-Vicente -- Mimicking muscle stem cell quiescence in culture : methods for synchronization in reversible arrest / Reety Arora, Mohammed Rumman, Nisha Venugopal, Hardik Gala, and Jyotsna Dhawan -- Methods for observing and quantifying muscle satellite cell motility and invasion in vitro / Dane K. Lund, Patrick McAnulty, Ashley L. Siegel, and DDW Cornelison -- Effects of macrophage conditioned-medium on murine and human muscle cells : analysis of proliferation, differentiation, and fusion / Marielle Saclier, Marine Theret, Rémi Mounier, and Bénédicte Chazaud -- Optimization of stellite cell culture through biomaterials / Sadegh Davoudi and Penney M. Gilbert -- Systematic identification of genes regulating muscle stem cell self-renewal and differentiation / Krishnamoorthy Sreenivasan, Thomas Braun, and Johnny Kim -- Bioinformatics for novel long intergenic noncoding RNA (lincRNA) identification in skeletal muscle cells / Xianlu Peng, Kun Sun, Jiajian Zhou, Hao Sun, and Huating Wang.
  • Digital
    Mari Dezawa.
    Springer2018
    This book provides the first comprehensive account of multilineage-differentiating stress-enduring (Muse) cells, a pluripotent and non-tumorigenic subpopulation of mesenchymal stem cells (MSCs) that have the ability to detect damage signals, migrate to damaged sites, and spontaneously differentiate into cells compatible with the affected tissue, thereby enabling repair of all tissue types. The coverage encompasses everything from the basic properties of Muse cells to their tissue repair effects and potential clinical applications--for example, in acute myocardial infarction, stroke, skin injuries and ulcers, renal failure, and liver disease. An important technical chapter provides a practical and precise protocol for the isolation of Muse cells, which will enable readers to use Muse cells in their own research. In offering fascinating insights into the strategic organization of the body's reparative function and explaining how full utilization of Muse cells may significantly enhance the effectiveness of MSC treatment, the book will be of high value for Ph.D. students, postdocs, basic researchers, clinical doctors, and industrial developers.
  • Digital
    Swati Palit Deb, Sumitra Deb, editor.
    Springer2014
    This book provides the readers with an overview of research on p53, which has been shown to play a role in numerous crucial biological pathways in normal and cancer cells. Leading scientist in the field, who have all made direct contributions to the understanding of the molecular events underpinning p53 function, have been invited to contribute the various chapters, which discuss the current knowledge of the signaling cascades that are activated by mutations in p53 and overexpression of MDM2, frequently found in human cancer, and are major causes of oncogenesis. This book features chapters on the molecular basis of oncogenesis induced by gain of function mutation of p53, signaling pathways induced by MDM2 overexpression, control of mutant or wild-type p53 function by MDM2 and MDMX, p53 mutation in hereditary cancer, and structural aspects that activate mutant p53 which can be targeted by drug therapy. This book should be useful for scientists at all levels.
  • Digital
    edited by Laura Soucek, Nicole M. Sodir.
    Springer2013
  • Digital
    edited by Siamon Gordon, Emeritus Professor of Cellular Pathology, Sir William Dunn School of Pathology, Oxford, United Kingdom.
    Am Soc Microbiol2017
  • Digital
    Dennis V. Cokkinos, editor.
    Springer2019
    This timely book reveals an integrated approach to myocardial preservation focusing on translational research and clinical applications. Chapters cover both the mechanisms of heart failure in addition to therapeutic considerations, including forms of cardiac cell death, cardiac remodelling and cardiac regeneration. Potential future research directions are also proposed, enabling the reader to gain a broad in-depth understanding of the topic. Myocardial Preservation: Translational Research and Clinical Application presents a thorough review of myocardial preservation. Its comprehensive approach provides a valuable reference for cardiology researchers and practising and trainee cardiologists seeking new insight to the topic.
  • Digital
    edited by Joseph X. DiMario.
    Springer2012
    Isolation and characterization of human fetal myoblasts/ Ariya D. Lapan and Manuela Gussoni -- Skeletal muscle satellite cells : background and methods for isolation and analysis in a primary culture system / Maria Elena Danoviz and Zipora Yablonka-Reuveni -- Isolation of muscle stem cells by fluorescence activated cell sorting cytometry / Alessandra Pasut, Paul Oleynik, and Michael A. Rudnicki -- Mouse and human mesoangioblasts : isolation and characterization from adult skeletal muscles / Mattia Quattrocelli [and others] -- Direct electrical stimulation of myogenic cultures for analysis of muscle fiber type control / Eric J. Cavanaugh, Jennifer R. Crew, and Joseph X. DiMario -- Single muscle-fiber isolation and culture for cellular, molecular, pharmacological, and evolutionary studies / Judy E. Anderson, Ashley C. Wozniak, and Wataru Mizunoya -- Somite unit chronometry to analyze teratogen phase specificity in the paraxial mesoderm / Sarah J. Venters and Charles P. Ordahl -- Analysis of skeletal muscle development in drosophila / Ginny R. Morriss [and others] -- Immunocytochemistry to study myogenesis in zebrafish / Nathan C. Bird, Stefanie E. Windner, and Stephen H. Devoto -- Immunofluorescent localization of proteins in Caenorhabditis elegans muscle / Kristy J. Wilson, Hiroshi Qadota, and Guy M. Benian -- Resistance loading and signaling assays for oxidative stress in rodent skeletal muscle / Stephen E. Alway and Robert G. Cutlip -- Analysis of skeletal muscle hypertrophy in models of increased loading / Sue C. Bodine and Keith Baar -- Protein overexpression in skeletal muscle using plasmid-based gene transfer to elucidate mechanisms controlling fiber size / Chia-Ling Wu and Susan C. Kandarian -- In vivo measurement of muscle protein synthesis rate using the flooding dose technique / Marta L. Fiorotto, Horacio A. Sosa Jr., and Teresa A. Davis -- Recombinant adeno-associated viral vector production and purification / Jin-Hong Shin, Yongping Yue, and Dongsheng Duan -- Generation of lentiviral vectors for use in skeletal muscle research / Christophe Pichavant and Jacques P. Tremblay -- Generating tamoxifen-inducible Cre alleles to investigate myogenesis in mice / Christoph Lepper and Chen-Ming Fan -- Gene profiling studies in skeletal muscle by quantitative real-time polymerase chain reaction assay / Shephali Bhatnagar, Siva K. Panguluri, and Ashok Kumar -- Analysis of lipid profiles in skeletal muscles / Vassilis Mougios and Anatoli Petridou -- Proteomic analysis of dystrophic muscle / Caroline Lewis, Philip Doran, and Kay Ohlendieck -- Detection of calcium release via ryanodine receptors / Jerry P. Eu and Gerhard Meissner -- Measurement of calcium release due to inositol trisphosphate receptors in skeletal muscle / Mariana Casas, Francisco Altamirano, and Enrique Jaimovich -- Detection of calcium sparks in intact and permeabilized skeletal muscle fibers / Noah Weisleder, Jingsong Zhou, and Jianjie Ma -- Analysis of calcium transients in cardiac myocytes and assessment of the sarcoplasmic reticulum Ca(2+)-ATPase contribution / Anand Mohan Prasad and Giuseppe Inesi -- Analysis of muscle gene transcription in cultured skeletal muscle cells / Charis L. Himeda, Phillip W.L. Tai, and Stephen D. Hauschka -- Analysis of fiber-type differences in reporter gene expression of [beta]-gal transgenic muscle / Phillip W.L. Tai [and others] -- Determination of gene promoter activity in skeletal muscles in vivo / Sarah M. Senf and Andrew R. Judge -- Determination of miRNA targets in skeletal muscle cells / Zhan-Peng Huang, Ramón Espinoza-Lewis, and Da-Zhi Wang -- ShRNA-mediated gene knockdown in skeletal muscle / Muriel Golzio, Jean-Michel Escoffre, and Justin Teissié -- Detection of NF-[kappa]B activity in skeletal muscle cells by electrophoretic mobility shift analysis / Jason M. Dahlman and Denis C. Guttridge -- Isolation of nuclei from skeletal muscle satellite cells and myofibers for use in chromatin immunoprecipitation assays / Yasuyuki Ohkawa [and others] -- An improved restriction enzyme accessibility assay for analyzing changes in chromatin structure in samples of limited cell number / Yasuyuki Ohkawa [and others] -- ChIP-enriched in silico targets (ChEST), a ChIP-on-chip approach applied to analyzing skeletal muscle genes / Guillaume Junion and Krzysztof Jagla.
  • Digital/Print
    David G. Capco, Yongsheng Chen, editors.
    Digital : Springer2014
    Print2014
    Presence in, and release of, nanomaterials from consumer products / Yu Yang and Paul Westerhoff -- Nanoparticle aggregation: principles and modeling / Wen Zhang -- Influences of nanomaterials on the barrier function of epithelial cells / Shariq Ali and Erik Rytting -- Engineered nanoparticles induced brush border disruption in a human model of the intestinal epithelium / James J. Faust, Benjamin M. Masserano, Adam H. Mielke, Anup Abraham, and David G. Capco -- Nanoparticles: cellular uptake and cytotoxicity / Isaac M. Adjei, Blanka Sharma and Vinod Labhasetwar -- Atomic force microscopy study of the interaction of DNA and nanoparticles / Kungang LI, Songyan Du, Steven Van Ginkel, and Yongsheng Chen -- Intracellular signal modulation by nanomaterials / Salik Hussain, Stavros Garantziotis, Fernando Rodrigues-Lima, Jean-Marie Dupret, Armelle Baeza-Squiban, Sonja Boland -- Nanomaterials: impacts on cells and cell organelles / Željka Krpetić, Sergio Anguissola, David Garry, Philip Kelly and Kenneth A. Dawson -- Design, synthesis, and functionalization of nanoparticles for therapeutic drug delivery / Taraka Sai Pavan Grandhi and Kaushal Rege -- Preparation of nanoscale pulmonary drug delivery formulations by spray drying / Adam Bohr, Christian A. Ruge, and Moritz Beck-Broichsitter -- Nanomedicine: the promise and challenges in cancer chemotherapy / Youssef W. Naguib and Zhengrong Cui -- Transgenerational effects of NMs / Anna Poma, Sabrina Colafarina, Gabriella Fontecchio, and Giuseppe Chichiricco -- Stem cells and nanomaterials / Maria-Claude Hofmann.
  • Digital
    Jacques Zimmer, editor.
    Springer2010
  • Digital
    editors, Michael T. Lotze, Angus W. Thomson.
    ScienceDirect2010
    Chapter 1, Developmental stages and pathways of NK cell maturation / Bartosz Grzywacz, Jeffery S. Miller, Michael R. Verneris -- Chapter 2. Developmental origins of thymus-derived natural killer cells / James R. Carlyle, Juan C. Zúñiga-Pflücker -- Chapter 3. Interferon-producing killer dendritic cells (IKDC) / Franck Housseau -- Chapter 4. Natural killer cells in cancer / Petter Höglund, Hans-Gustaf Ljunggren -- Chapter 5. NK cell immune recognition: NKG2D ligands and stressed cells / Carlos López-Larrea, Alejandro López-Soto, Segundo González -- Chapter 6. NK cell KIR heterogeneity and evolution / Makoto Yawata, Nobuyo Yawata -- Chapter 7. Signalling events in natural killer cells / P.K. Epling-Burnette, Sheng Wei, Julie Y. Djeu -- Chapter 8. Human natural killer cell development / Edward L. Briercheck, Aharon G. Freud, Michael A. Caligiuri -- Chapter 9. Isolation, culture and propagation of natural killer cells / Michael Magee, Lazar N. Vujanovic, Lisa H. Butterfield, Nikola L. Vujanovic -- Chapter 10. The localization and migration of natural killer cells in health and disease / Vasileios Bekiaris, Peter J.L. Lane -- Chapter 11. Receptors on NK cells / Noam Stern-Ginossar, Ofer Mandelboim -- Chapter 12. Development and testing of NK cell lines / Hans Klingemann -- Chapter 13. NK cell-derived cytokines and delivery: NK cell synapses / Claudia Semino, Anna Rubartelli -- Chapter 14. NK cells as recipients of cytokine signals / Tara J. Loux, Michael T. Lotze, Herbert J. Zeh III -- Chapter 15. NK cells and chemokines / Angela Gismondi, Giovanni Bernardini, Angela Santoni -- Chapter 16. Natural killer cytolytic activity / Lesley R. de Armas, Eckhard R. Podack -- Chapter 17. Illuminating intranodal natural killer cell behaviour using two-photon microscopy / Kym R. Garrod, Michael D. Cahalan -- Chapter 18. Dendritic cells and NK cells / Amy K. Wesa, Robbie B. Mailliard -- Chapter 19. NK and NKT cells: The innate-adaptive interface including humoral responses / Jeff Subleski, Jonathan M. Weiss, Robert H. Wiltrout, John R. Ortaldo -- Chapter 20. NK cell and endothelial cell interactions / Angela Gismondi, Claudia Carlino, Helena Stabile, Angela Santoni -- Chapter 21. Natural killer cell/epithelial interactions / Taisuke Ito, Masao Takei, Ralf Paus -- Chapter 22. NK cell-T cell interactions / Benedict J. Chambers -- Chapter 23. NK cells and NKT cells in cutaneous disorders / Anna Balato, Anthony A. Gaspari -- Chapter 24. Natural killer cells in the respiratory tract / Borna Mehrad -- Chapter 25. Intestinal natural killer cells / Martin R. Goodier -- Chapter 26. Natural killer cells in the liver / Shiguang Qian, Jia Fan, Shuang-Jian Qiu, Yong-sheng Xiao, Lina Lu -- Chapter 27. Natural killer cells in the spleen and lymph node / Alex Karlsson-Parra -- Chapter 28. Nature killer cells in the central nervous system / Fu-Dong Shi, Richard M. Ransohoff -- Chapter 29. NK cells in the eye / Jerry Y. Niederkorn -- Chapter 30. NK cells and reproduction / Ashley Moffett, Victoria H. Male -- Chapter 31. Identification of natural killer cells in tissues and their isolation / Paolo Carrega, Erika Montalto, Guido Ferlazzo -- Chapter 32. Natural killer cell immune regulation: Coordination of immune function in tissues / Lorenzo Moretta, Alessandro Moretta -- Chapter 33. Natural killer cells and transplantation / Benjamin M. Matta, Angus W. Thomson -- Chapter 34. Natural killer cells and autoimmunity / Christian Münz -- Chapter 35. Interaction of NK cells with bacteria / Kenji Chamoto, Daiko Wakita, Shinichi Koizumi, Kazutaka Masuko, Takayuki Ikeda, Rieko Mitamura, Takashi Nishimura -- Chapter 36. Natural killer cells and human immunodeficiency virus / Domenico Mavilio, Anne Hosmalin, Daniel Scott-Algara -- Chapter 37. Cytomegalovirus infection and NK cells / Jerome D. Coudert, Christopher E. Andoniou, Mariapia A. Degli-Esposti -- Chapter 38. Natural killer cells in allergy / Syh-Jae Lin, Yen-Chang Lee, Dah-Chin Yan, Ming-Ling Kuo -- Chapter 39. Natural killer cells in wound healing / Jussi Liippo, Mervi Toriseva, Veli-Matti Kähäri -- Chapter 40. Natural killer cells in atherosclerosis / Godfrey S. Getz, Catherine A. Reardon -- Chapter 41. Natural killer cells in leukaemia / Jérôme Rey, Cyril Fauriat, Alessandro Moretta, Régis Costello, Daniel Olive -- Chapter 42. Natural killer cells and allogeneic haematopoietic cell transplantation / Andrea Velardi -- Chapter 43. Clinical trials of NK cells for cancer / Sarah Cooley, Jeffrey S. Miller -- Chapter 44. Natural killer cells and hepatitis C virus infection / Michael A. Nalesnik, Tatsuya Kanto -- Chapter 45. Natural killer cells and the biology of parasitism / Marianne A. Bryan, Karen A. Norris -- Chapter 46. Lymphoproliferative disorders of natural killer cells / William G. Morice -- Chapter 47. Natural killer cell induction of tolerance / Lina Lu, Alexandra Y. Zhang, William L. Camp, Shiguang Qian -- Chapter 48. Genetic engineering of natural killer cells / Stephen R. Goding, Per H. Basse -- Chapter 49. NK cell-mediated target cell death / Beverly Z. Packard, Akira Komoriya, David S. Ucker -- Chapter 50. NK-cell tracking using non-invasive imaging modalities / Priyanka Jha, Verena Reinhart, Christiane Knopp, Siyuan Liu, Winfried S. Wels, Michael F. Wendland, Heike Daldrup-Link.
  • Digital
    Masaki Terabe, Jay A. Berzofsky, editors.
    Springer2012
    Introduction : mechanisms of NKT-cell-mediated adjuvant activity and function of iPS-derived NKT cells / Masaru Taniguchi ... [et al.] -- Structure and recognition of antigens for invariant NKT cells / Bo Pei and Mitchell Kronenberg -- Invariant NKT cell-based vaccine strategies / John-Paul Jukes ... [et al.] -- Immune regulation of tumor immunity by NKT cells / Jessica J. O'Konek, Jay A. Berzofsky, and Masaki Terabe -- The regulation of CD1d+ and CD1d- tumors by NKT cells : the roles of NKT cells in regulating CD1d+ and CD1d- tumor immunity / Jianyun Liu, Gourapura J. Renukaradhya, and Randy R. Brutkiewicz -- DC-based immunotherapy targeting NKT cells / Shin-ichiro Fujii and Kanako Shimizu -- Therapeutic approaches utilising NKT cells / Stephen R. Mattarollo and Mark J. Smyth -- NKT cells or cancer patients and how models can inform therapeutic plans / Mark A. Exley, Lydia Lynch, and Michael Nowak -- Understanding the role of natural killer T cells in hematologic malignancies : progress and challenges / Natalia Neparidze and Madhav V. Dhodapkar -- Clinical trials with alpha-galactosylceramide (KRN7000) in advanced cancer / Famke L. Schneiders ... [et al.] -- Clinical trials of invariant natural killer T cell-based immunotherapy for cancer / Shinichiro Motohashi, Yoshitaka Okamoto, and Toshinori Nakayama.
  • Digital
    edited by Kimberly McCall, Charles Klein.
    Springer2013
    Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. -- Publisher website.
  • Digital
    edited by Paul A. Trainor.
    ScienceDirect2014
    This book summarizes discoveries of historical significance and provides in-depth, current analyses of the evolution of neural crest cells, their contribution to embryo development, and their roles in disease. In addition, prospects for tissue engineering, repair and regeneration are covered, offering a timely synthesis of the current knowledge in neural crest cell research.
  • Digital
    Mahendra S. Rao, Melissa Carpenter, Mohan C. Vemuri, editors.
    Springer2012
    Defining neural stem cells and their role in normal development of the nervous system / Sally Temple -- Embryonic stem cells and neurogenesis / Robin L. Wesselschmidt -- The cellular and molecular properties of multipotent neural stem cells / Scott R. Hutton and Larysa H. Pevny -- Multipotent stem cells in the embryonic nervous system / Chian-Yu Peng ... [et al.] -- Neural crest formation and diversification / Marcos S. Simões-Costa ... [et al.] -- Glial nature of adult neural stem cells : neurogenic competence in adult astrocytes / William W. Hwang, Arturo Alverez-Buylla and Daniel A. Lim -- Neural stem cell death regulation in nervous system development and disease / K.C. Walls and Kevin A. Roth -- Stem cells of adult olfactory epithelium / James E. Schwob, Woochan Jang and Eric H. Holbrook -- Retinal stem cells / Carolina B. Del Debbio ... [et al.] -- Transdifferentiation in the nervous system / Ying Liu and Mahendra S. Rao -- Neural and dopaminergic differentiation of human pluripotent stem cells / Olga Momcilovic and Xianmin Zeng -- Mobilization of neural stem cells in the adult central nervous system / Harish Babu, Theo D. Palmer and Fred H. Gage -- Stem cell pathways in brain tumors / Justin D. Lathia ... [et al.].
  • Digital
    edited by Brent A. Reynolds, Loic P. Deleyrolle.
    Springer2013
    Culturing Fetal Precursor Cells Using Free Floating Serum-Free Conditions / Andrew Chojnacki and Samuel Weiss -- In Vitro Expansion of Fetal Neural Progenitors as Adherent Cell Lines / Steven M. Pollard -- Isolate and Culture Precursor Cells from the Adult Periventricular Area / Chiara Cavazzin, Margherita Neri, and Angela Gritti -- Culturing and Expansion of Precursor Cells from the Adult Hippocampus / Dhanisha J. Jhaveri, Boris W. Prosper, and Perry F. Bartlett -- Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord / Jean-Philippe Hugnot -- Culturing and Expansion of "Clinical Grade" Precursors Cells from the Fetal Human Central Nervous System / Maurizio Gelati [and five others] -- Isolating and Culturing of Precursor Cells from the Adult Human Brain / Florian A. Siebzehnrubl and Dennis A. Steindler -- Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord / Luc Bauchet [and five others] -- Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny / Hassan Azari -- Isolation of Adult Stem Cells from the Human Olfactory Mucosa / François Féron [and three others] -- Enumerating Stem Cell Frequency: Neural Colony Forming Cell Assay / Sharon A. Louis and Carmen K.H. Mak -- Flow Cytometry of Neural Cells / Geoffrey W. Osborne -- Neonatal Transplant in Hypoxic Injury / Tong Zheng and Michael D. Weiss -- Isolation and Purification of Self-Renewable Human Neural Stem Cells for Cell Therapy in Experimental Model of Ischemic Stroke / Ricardo L. Azevedo-Pereira and Marcel M. Daadi -- Transplantation of Fetal Midbrain Dopamine Progenitors into a Rodent Model of Parkinson's Disease / Lachlan H. Thompson and Clare L. Parish -- Distribution of Neural Precursor Cells in the Adult Mouse Brain / Daniel G. Blackmore and Rodney L. Rietze -- Identifying Neural Progenitor Cells in the Adult Human Brain / Thomas I.H. Park [and seven others] -- Clinical Trials for the Treatment of Spinal Cord Injury: Not So Simple / Alan Mackay-Sim and François Féron.
  • Digital
    edited by Leslie P. Weiner.
    Springer2008
    Definitions and criteria for stem cells / Leslie P. Weiner -- Neural stem cells and the future treatment of neurological diseases : raising the standard / Jaime Imitola and Samia J. Khoury -- Neural differentiation of human embryonic stem cells / Mirella Dottori and Martin F. Pera -- Generating neurons from stem cells / Andreas Androutsellis-Theotokis ... [et al.] -- Isolation of neural stem and precursor cells from rodent tissue / Yuanyuan Wu ... [et al.] -- Adult rodent spinal cord-derived neural stem cells : isolation and characterization / Lamya S. Shihabuddin -- Adult neural stem cells / Rossella Galli, Angela Gritti, and Angelo L. Vescovi -- Olfactory ensheathing cells : isolation and culture from the neonatal olfactory bulb / Susan C. Barnett and A. Jane Roskams -- Culturing olfactory ensheathing cells from the mouse olfactory epithelium / Miranda Richter, Kathryn Westendorf, and A. Jane Roskams -- Production and characterization of immortal human neural stem cell line with multipotent differentiation property / Seung U. Kim ... [et al.] -- Preparation of neural progenitors from bone marrow and umbilical cord blood / Shijie Song and Juan Sanchez-Ramos -- Production of neurospheres from CNS tissue / Gregory P. Marshall II ... [et al.] -- Nuclear transfer to study the nuclear reprogramming of human stem cells / Shigeo Saito ... [ et al.] -- Clonal analyses and cryopreservation of neural stem cell cultures / Angela Gritti, Rossella Galli, and Angelo L. Vescovi -- Telomere neurobiology / Mark P. Mattson, Peisu Zhang, and Aiwu Cheng -- Chromosomal mosaicism in neural stem cells / Suzanne E. Peterson ... [et al.] -- In vitro assays for neural stem cell differentiation : induction of dopaminergic phenotype / Marcel M. Daadi -- In vitro migration assays of neural stem cells / Pascale Durbec .. [et al.] -- Measuring apoptosis in neural stem cells / Brett T. Lund and Eve E. Kelland -- Markers of adult neural stem cells / Eric Wexler -- Differential gene expression in ES-derived neural stem cells by using RT-PCR / Nicole Slawny, Crystal Pacut, and Theresa E. Gratsch -- Microfluidic devices for high-throughput gene expression profiling of single hESC-derived neural stem cells / Yan Chen and Jiang F. Zhong -- Electroporation strategies for genetic manipulation and cell labeling / Terence J. Van Raay, Rhonda T. Lassiter, and Michael R. Stark -- Novel and immortalization-based protocols for the generation of neural CNS stem cell lines for gene therapy approaches / Luciano Conti and Elena Cattaneo -- Identification of newborn cells by BrdU labeling and immunocytochemistry in vivo / Sanjay S. Magavi and Jeffrey D. Macklis -- Immunocytochemical analysis of neuronal differentiation / Sanjay S. Magavi and Jeffrey D. Macklis -- Neuroanatomical tracing of neuronal projections with Fluoro-Gold / Lisa A. Catapano, Sanjay S. Magavi, and Jeffrey D. Macklis -- Labeling stem cells in vitro for identification of their differentiated phenotypes after grafting into the CNS / Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore -- Optimizing stem cell grafting into the CNS / Scott R. Whittemore ... [et al.] -- Routes of stem cell administration in the adult rodent / Alison E. Willing ... [et al.]
  • Digital/Print
    Mieczyslaw Pokorski, editor.
    Digital : Springer2013
    Print2013
    Altered histone deacetylase activity and iNOS expression in cells isolated from induced sputum of COPD patients treated with tiotropium -- Involvement of NF-[kappa]B and muscle specific E3 ubiquitin ligase MuRF1 in cigarette smoke-induced catabolism in C2 myotubes -- Siglec-8 in induced sputum of COPD patients -- Essential amino acid leucine and proteasome inhibitor MG132 attenuate cigarette smoke induced catabolism in C2 myotubes -- Smoking denial in cardiovascular disease studies -- Differential effects of kainic acid lesions in medullary raphe on cough and sneeze in anesthetized rabbits -- Detection of Chlamydophila pneumoniae antigens in patients with chronic cough -- Antioxidant activity of herbal polysaccharides and cough reflex -- Cytoglobin and neuroglobin in the human brainstem and carotid body -- Flow cytometry in the diagnosis of influenza -- Accuracy of rapid influenza detection test in diagnosis of influenza A and B viruses in children less than 59 months old -- Virological monitoring of influenza activity and influenza-like illness in the epidemic season 2011-2012 in Poland -- Incidence of Chlamydophila pneumoniae infection in children during 2007-2010 -- Clinical features and outcomes of influenza A and B infections in children -- Respiratory complications in children hospitalized with Varicella -- Hyponatremia in children hospitalized due to pneumonia -- In vitro sensitivity of Acinetobacter baumannii and Pseudomonas aeruginosa to carbapenems among intensive care unit patients -- Prevalence of acute respiratory tract diseases among soldiers deployed for military operations in Iraq and Afghanistan -- In vitro susceptibility of Staphylococci and Enterococci to vancomycin and teicoplanin -- Sinusitis in people living in the Medieval Ages -- Immunization coverage against capsular bacteria in splenectomized patients -- Umbilical cord blood gas content, postnatal state of neonates, and lactation after caesarean and natural childbirth -- Respiratory function in pregnant women -- Lung function in patients with gastro-esophageal reflux disease and respiratory symptoms -- Efficacy of noninvasive mechanical ventilation in obese patients with chronic respiratory failure -- Near-infrared hemoencephalography for monitoring blood oxygenation in prefrontal cortical areas in diagnosis and therapy of developmental dyslexia -- Occlusal stabilization splint therapy in orofacial pain and tension-type headache -- Dynamics of upper airways during the Müller maneuver in healthy subjects: a cine MRI study -- EMG analysis of human inspiratory muscle resistance to fatigue during exercise -- Assessment of temporomandibular joint disease -- Spirometry Day: a means to enhance social knowledge on respiratory diseases -- Lung function in divers -- Influence of traffic-related air pollutants on lung function -- Fractional exhaled nitric oxide in clinical trials: an overview -- Real-time breath analysis in type 2 diabetes patients during cognitive effort -- Exhaled nitric oxide in specific inhalation challenge -- Integration of simulated multipotential signals: the role of integration window width and of the number of spikes -- Functional and topographic concordance of right atrial neural structures inducing sinus tachycardia -- Occurrence of disseminated intravascular coagulation in 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced pneumonia in the rat -- Implementation of non-invasive methods in the diagnosis of diisocyanate-induced asthma -- Evaluation of a 4-steps-1-day whole body challenge protocol for the diagnosis of occupational asthma due to diisocyanates -- Occupational allergic respiratory diseases in garbage workers: relevance of molds and actinomycetes -- Th1, Th2, Th17, and regulatory cytokines in children with different clinical forms of allergy -- Soluble receptor for urokinase plasminogen activator in community-acquired pneumonia in children -- Hypersensitivity pneumonitis due to metalworking fluids: how to find the antigens -- Angiogenic axis angiopoietin-1 and angiopoietin-2/tie-2 in non-small cell lung cancer: a bronchoalveolar lavage and serum study -- Cancer incidence in pulmonary vasculitis -- Influence of delays in diagnosis and treatment on survival in small cell lung cancer -- Familial idiopathic pulmonary fibrosis -- High resolution computed tomography in 2-year follow-up of stage I sarcoidosis -- Altruistic aptitude: age-dependent influence of temperament and emotional intelligence -- Effect of socio-economic status on quality of life in people affected with respiratory allergy -- Exposure to paracetamol and antibiotics in early life and elevated risk of asthma in childhood -- Health behaviors and quality of life among patients with chronic respiratory disease -- Attitudes toward euthanasia among Polish physicians, nurses and people who have no professional experience with the terminally ill -- Death as a result of violent asphyxia in autopsy reports -- Respiratory and systemic infections in children with severe aplastic anemia on immunosupressive therapy.
  • Digital
    edited by Giovanni Manfredi, Hibiki Kawamata.
    Springer2011
    Introduction to neurodegenerative diseases and related techniques -- In vivo and in vitro determination of cell death markers in neurons -- Degenerating processes identified by electron microscopic immunocytochemical methods -- Isolation and culture of neurons and astrocytes from the mouse brain cortex -- Isolation and culture of postnatal spinal motoneurons -- Converting human pluripotent stem cells to neural tissue and neurons to model neurodegeneration -- Neural differentiation of induced pluripotent stem cells -- Developing yeast models of human neurodegenerative disorders -- Modeling dopamine neuron degeneration in Caenorhabditis elegans -- Role of the proteasome in fly models of neurodegeneration -- Modeling neurodegenerative diseases in zebrafish embryos -- Practical considerations of genetic rodent models for neurodegenerative diseases -- Modeling focal cerebral ischemia in vivo -- Pharmacological models of Parkinson's disease in rodents.
  • Digital
    Michail S. Davidoff, Ralf Middendorff, Dieter Müller, Adolf F. Holstein.
    Springer2009
  • Digital
    edited by Shohreh Amini, Martyn K. White.
    Springer2013
    General overview of neuronal cell culture -- Considerations for the use of SH-SY5Y neuroblastoma cells in neurobiology -- Cultured cell line models of neuronal differentiation : NT2, PC12 -- Murine teratocarcinoma-derived neuronal cultures -- Isolation and propagation of primary human and rodent embryonic neural progenitor cells and cortical neurons -- Mouse enteric neuronal cell culture -- Preparation of neural stem cells and progenitors : neuronal production and grafting applications -- Derivation of neuronal cells from fetal Normal Human Astrocyte (NHA) -- Slice culture modeling of Central Nervous System (CNS) viral infection -- Neurospheres and glial cell cultures : immunocytochemistry for cell phenotyping -- Transfection of neuronal cultures -- Lentiviral transduction of neuronal cells -- Compartmentalized neuronal cultures -- Quantitative assessment of neurite outgrowth over growth promoting or inhibitory substrates.
  • Digital
    edited by Laura Lossi and Adalberto Merighi.
    Springer2015
    Neuronal cell death : an overview of its different forms in central and peripheral neurons / Laura Lossi, Claudia Castagna, and Adalberto Merighi -- Cell volume regulation monitored with combined epifluorescence and digital holographic microscopy / Nicolas Pavillon and Pierre Marquet -- Flow cytometric analysis of DNA synthesis and apoptosis in central nervous system using fresh cell nuclei / Noelia López-Sánchez and José M. Frade -- Nuclear signs of pre-neurodegeneration / Fernando C. Baltanás ... [et al.] -- Multi-parametric O2 imaging in three-dimensional neural cell models with the phosphorescent probes / Ruslan I. Dmitriev and Dmitri B. Papkovsky -- Calcium imaging in neuron cell death / María Calvo, Carlos Villalobos, and Lucía Núñez -- Monitoring mitochondrial membranes permeability in live neurons and mitochondrial swelling through electron microscopy analysis / Macarena S. Arrázola and Nibaldo C. Inestrosa -- Real-time visualization of caspase-3 activation by fluorescence resonance energy transfer (FRET) / Silvia Alasia ... [et al.] -- Design and cloning of short hairpin RNAs (shRNAs) into a lentiviral silencing vector to study the function of selected proteins in neuronal apoptosis / Nadia Canu -- Genomic analysis using affymetrix standard microarray genechips (169 format) in degenerate murine retina / Sook Hyun Chung , Weiyong Shen , and Mark Gillies -- Genomic analysis of transcriptional changes underlying neuronal apoptosis / Sebastiano Cavallaro -- High-throughput cell death assays / Matthew E. Pamenter and Gabriel G. Haddad -- Staining of dead neurons by the golgi method in autopsy material / Stavros J. Baloyannis -- Image analysis algorithms for immunohistochemical assessment of cell death / Stan Krajewski ... [et al.] -- In vitro oxygen-glucose deprivation to study ischemic cell death / Carla I. Tasca, Tharine Dal-Cim, and Helena Cimarosti -- Laser microbeam targeting of single nerve axons in cell culture / Nicholas Hyun , Linda Z. Shi , and Michael W. Berns -- Real-time imaging of retinal cell apoptosis by confocal scanning laser ophthalmoscopy / Eduardo M. Normando ... [et al.] -- Targeted toxicants to dopaminergic neuronal cell death / Huajun Jin ... [et al.] -- Stem cells, neural progenitors, and engineered stem cells / Raj R. Rao and Shilpa Iyer -- Herpes simplex virus type 1 (HSV-1)-derived recombinant vectors for gene transfer and gene therapy / Peggy Marconi , Cornel Fraefel, and Alberto L. Epstein -- Herpes simplex virus type 1 (HSV-1)-derived amplicon vectors for gene transfer and gene therapy / Cornel Fraefel Peggy Marconi, and Alberto L. Epstein -- Bone marrow transplantation for research and regenerative therapies in the central nervous system / David Díaz, José Ramón Alonso, and Eduardo Weruaga -- Detection of activated caspase-8 in injured spinal axons by using fluorochrome-labeled inhibitors of caspases (FLICA) / Antón Barreiro-Iglesias and Michael I. Shifman -- Generation of zebrafish models by CRISPR/Cas9 genome editing / Alexander Hruscha and Bettina Schmid -- In vivo assessment of neuronal cell death in Drosophila / Pierre Dourlen -- Drosophila model for studying phagocytosis following neuronal cell death / Boris Shklyar, Flonia Levy-Adam, and Estee Kurant.
  • Digital
    edited by K. Kevin Pfister.
    ScienceDirect2016
  • Digital
    edited by William E. Armstrong, Jeffrey G. Tasker.
    Wiley2015
    Electrophysiology of magnocellular neurons in vivo -- Oxytocin neurons during suckling : lessons from organotypic cultures -- Peptidergic control of oxytocin and vasopressin neurons and its role in reproductive and hypertension-associated plasticity -- The osmotic control of vasopressin releasing neurons -- Function and localization of epithelial sodium channels in vasopressin and oxytocin neurons -- Visible markers of vasopressin and oxytocin activity and their use in identifying the neuronal activity of specific neuroendocrine cell types -- Neurophysiology of neurohypophysial terminals -- Neuronal-glia remodeling of the magnocellular system -- Dendritic release of the neuropeptides vasopressin and oxytocin -- Endocannabinoid modulation of synaptic inputs to magnocellular neurons -- Role of central vasopressin in the generation of multimodal homeostatic responses -- Elucidating the structure and function of gonadotropin-releasing hormone (GnRH) neuron dendrites -- Roles of estrogen and kisspeptin in controlling gonadotropin releasing hormone (GnRH) neuronal excitability -- Multiple-unit activity recording of the gonadotropin-releasing hormone pulse generator.
  • Digital
    Jessica A. Filosa.
    Atypon2015
    Optimal cerebral blood flow is coordinated by functional hyperemia and cerebral autoregulation. These processes ensure that the metabolic demands of the brain are met at all times. Both in vivo and in vitro studies support a role for astrocytes in the regulation of cerebral blood flow. In this we review the cellular mechanisms contributing to astrocyte-mediated vasodilation and vasoconstriction of parenchymal arterioles. Primarily, we discuss how activity-dependent changes in astrocytic Ca2+ contribute to the release of vasoactive signals involved in neurovascular coupling. Following the rise in astrocytic Ca2+ and phospholipase A2 activation, arachidonic acid is released and metabolized into multiple vasoactive signals (e.g., prostaglandins, epoxyeicosatrienoic acids, and 20-HETE). The level of arteriole tone along with the metabolic conditions of the tissue can alter the action of these signals leading to either vasodilation or vasoconstriction. Moreover, increased Ca2+ also activates large conductance Ca2+-activated K+ channels expressed in astrocytic endfeet processes. Depending on the K+ efflux concentration released, this pathway can also elicit vasodilation or vasoconstriction of parenchymal arterioles. Finally, we conclude by discussing recent divergent in vivo findings which question a role for astrocyte in functional hyperemia.
  • Digital
    edited by Mark T. Quinn, Frank R. DeLeo.
    Springer2014
    The role of neutrophils in the immune system : an overview / Harry L. Malech, Frank R. DeLeo, and Mark T. Quinn -- Isolation of human neutrophils from venous blood / William M. Nausef -- Neutrophil isolation from nonhuman species / Daniel W. Siemsen ... [and 7 others] -- Collection of in vivo transmigrated neutrophils from human skin / Karin Christenson ... [and 6 others] -- Subcellular fractionation of human neutrophils and analysis of subcellular markers / Stine Novrup Clemmensen, Lene Udby, and Niels Borregaard -- Rho family and Rap GTPase activation assays / Richard T. Jennings and Ulla G. Knaus -- Measurement of phospholipid metabolism in intact neutrophils / Susan Sergeant and Linda C. McPhail -- Optical methods for the measurement and manipulation of cytosolic calcium signals in neutrophils / Maurice B. Hallett, Maha Al-Jumaa, and Sharon Dewitt -- Analysis of electrophysiological properties and responses of neutrophils / Deri Morgan and Thomas E. DeCoursey -- Assessment of neutrophil apoptosis / David A. Dorward ... [and 3 others] -- Microinjection methods for neutrophils / Iraj Laffafian ... [and 3 others] -- Generation of functionally mature neutrophils from induced pluripotent stem cells / Colin L. Sweeney ... [and 6 others] -- Neutrophil migration through extracellular matrix / Richard T. Jennings and Ulla G. Knaus -- Spinning disk confocal imaging of neutrophil migration in zebrafish / Pui-Ying Lam ... [and 4 others] -- Detection of bidirectional signaling during integrin activation and neutrophil adhesion / Stuart M. Altman, Neha Dixit, and Scott I. Simon -- Immunofluorescence and confocal microscopy of neutrophils / Lee-Ann H. Allen -- Expressin of genetically encoded fluorescent probes to monitor phospholipid dynamics in live neutrophils / Benjamin E. Steinberg, Marco A.O. Magalhaes, and Sergio Grinstein -- Quantitative assessment of neutrophil phagocytosis using flow cytometry / Pontus Nordenfelt -- Analysis of neutrophil bactericidal activity / Heather A. Parker ... [and 4 others] -- Induction and quantification of neutrophil extracellular traps / Alejandro Sanchez Gonzalez ... [and 3 others] -- Measurement of respiratory burst products, released or retained, during activation of professional phagocytes / Johan Bylund ... [and 4 others] -- Cell-free NADPH oxidase activation assays : "In vitro veritas" / Edgar Pick -- Assessment of priming of the human neutrophil respiratory burst / Margarita Hurtado-Nedelec ... [and 4 others] -- Affinity pruification and reconstitution of human phagocyte flavocytochrome b for detection of conformational dynamics in the membrane / Marcia Riesselman and Algirdas J. Jesaitis -- Evaluation of p47phox phosphorylation in human neutrophils using phospho-specicfic antibodies / Sahra Amel Belambri, Pham My-Chan Dang, and Jamel El-Benna -- Genome-scale transcript analyses with human neutrophils / Scott D. Kobayashi, Daniel E. Sturdevant, and Frank R. DeLeo -- Fast and accurate quantitative analysis of cytokine gene expression in human neutrophils / Nicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni -- High-purity neutrophil isolation from human peripheral blood and saliva for transcriptome analysis / Flavia S. Lakschevitz and Michael Glogauer -- Detection of intact transciption factors in human neutrophils / Patrick P. McDonald and Richard D. Ye -- Disorders of neutrophil function : an overview / Mary C. Dinauer -- Diagnostic assays for chronic granulomatous disease and other neutrophil disorders / Houda Zghal Elloumi and Steven M. Holland -- Diagnostic assays for myeloperoxidase and myeloperoxidase deficiency / William M. Nauseef.
  • Digital
    Avi Ma'ayan, Ben D. MacArthur, editors.
    Springer2012
    Gene Expression Profiling and Regulatory Networks in Single Cells / Fumio Arai, Kentaro Hosokawa, Yoshiko Matsumoto, Hirofumi Toyama and Toshio Suda -- Cell Fate Regulatory Networks / Sonya J. Ridden and Ben D. MacArthur -- Noise in Regulatory Networks / Srinandan Dasmahapatra -- Generalised Modelling in Systems Biology / Thilo Gross -- Relative Quantification Mass Spectrometry Using iTRAQ Isobaric Tags / Richard D. Unwin, Emma Carrick and Anthony D. Whetton -- Deciphering Protein Complexes and Protein Interaction Networks for Stem Cell Pluripotency / Jianlong Wang -- Omics Era in Stem Cell Research: Data Integration of Multi-regulatory Layers / Ana Sevilla, Benjamin Trinite and Ihor R. Lemischka -- High Throughput Affinity Purification and Mass Spectrometry to Determine Protein Complex Interactions / Anna Malovannaya, Rainer B. Lanz, Bert W. O'Malley and Jun Qin -- Network Pharmacology to Aid the Drug Discovery Process / Ruth Dannenfelser, Huilei Xu, Catherine Raimond and Avi Ma'ayan -- What Can Networks Do for You? / Bernhard M. Schuldt, Franz-Josef Müller and Andreas A. Schuppert.
  • Digital/Print
    edited by Enrique Cadenas, Lester Packer.
    Digital : ScienceDirect2002
    Print2002
  • Digital
    Muller Fabbri, editor.
    Springer2014
    Introduction / Muller Gabbri -- Biogenesis and physiology of microRNAs / Carlos A. Melo and Sonia A. Melo -- Detecting noncoding RNA expression : from arrays to next-generation sequencing / Catalina Perdomo, Joshua Campbell, and Frank Schembri -- MicroRNAs in solid tumors / Jin Wang and Subrata Sen -- MicroRNAs in hematologic malignancies / Johannes Bloehdorn ... [et al.] -- miRNAs as cancer biomarkers / Edward R. Sauter -- Unraveling the complex network of interactions between noncoding RNAs and epigenetics in cancer / Veronica Davalos and Manel Esteller -- MicroRNA SNPs in cancer / Yujing LI and Peng Jin -- Bioinformatics approaches to the study of MircoRNAs / Ravi Gupta and Ramana V. Davuluri -- Beyond miRNAs : role of other noncoding RNAs in cancer / Roxana S. Redis and George A. Calin -- Translational implication for noncoding RNA in cancer / Duaa Dakhallah, Melissa Piper, and S. Patrick Nana-Sinkam.
  • Digital
    edited by Hugo J. Bellen, Shinya Yamamoto.
    Springer2014
    Introduction to notch signaling / Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen -- Genetic screens to identify new notch pathway mutants in Drosophila / Nikolaos Giagtzoglou -- Structure-function analysis of Drosophila notch using genomic tescue transgenes / Jessica Leonardi and Hamed Jafar-Nejad -- Overview of genetic tools and techniques to study notch signaling in mice / Thomas Gridley and Andrew K. Groves -- Immunohistochemical tools and techniques to visualize notch in Drosophila melanogaster / Emiliana Tognon and Thomas Vaccari -- Antibody uptake assay and in vivo imaging to study intracellular trafficking of notch and delta in Drosophila / Lydie Couturier and François Schweisguth -- Tracking trafficking of notch and its ligands in mammalian cells / Patricia Chastagner and Christel Brou -- Visualizing notch signaling in vivo in Drosophila tissues / Benjamin E. Housden, Jinghua Li, and Sarah J. Bray -- Monitoring notch activity in the mouse / Swananda Marathe and Lavinia Alberi -- Notch signaling assays in Drosophila cultured cell lines / Jinghua Li, Benjamin E. Housden, and Sarah J. Bray -- Monitoring notch activation in cultured mammalian cells : transcriptional reporter assays / Ma. Xenia G. Ilagan and Raphael Kopan -- Monitoring notch activation in cultured mammalian cells : luciferase complementation imaging assays / Ma. Xenia G. Ilagan and Raphael Kopan -- Visualization of notch signaling oscillation in cells and tissues / Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama -- Proteomic analysis of the notch interactome / K. G. Guruharsha ... [et al.] -- Bacterial expression and in vitro refolding of limited fragments of the notch receptor and its ligands / Pat Whiteman, Christina Redfield, and Penny A. Handford -- Analyzing the posttranslational modification status of notch using mass spectrometry / Shinako Kakuda and Robert S. Haltiwanger -- Assay to probe proteolytic processing of notch by [gamma]-secretase / Lutgarde Serneels, Ina Tesseur, and Bart De Strooper -- Analyzing the nuclear complexes of notch signaling by electrophoretic mobility shift assay / Kelly L. Arnett and Stephen C. Blacklow -- Identifying direct notch transcriptional targets using the GSI-washout assay / Will Bailis, Yumi Yashiro-Ohtani, and Warren S. Pear -- Probing the epigenetic status at notch target genes / Robert Liefke and Tilman Borggrefe -- Notch-ligand binding assays in Drosophila cells / Aiguo Xu and Kenneth D. Irvine -- Modeling notch signaling : a practical tutorial / Pau Formosa-Jordan and David Sprinzak -- Small molecules that inhibit notch signaling / Gerdien E. De Kloe and Bart De Strooper -- Application and evaluation of anti-notch antibodies to modulate notch signaling / Wendy R. Gordon and Jon C. Aster -- Application of anti-ligand antibodies to inhibit notch signaling / Jun-ichiro Koga and Masanori Aikawa.
  • Digital/Print
    Christopher Ahern, Stephan Pless, editors.
    Digital : Springer2015
    Print2015
    Introduction -- Engineered ionized side chains -- Cysteine modification: probing channel structure, function, and conformational change -- Functional site-directed fluorometry -- Bioreactive tethers -- Flipping the photoswitch: ion channels under light control -- Incorporation of non-canonical amino acids -- Index.
  • Digital
    Stefan Fruehauf, W. Jens Zeller, Gary Calandra, editors.
    Springer2012
    Part 1. Basic Science and Experimental Studies on Stem Cell Mobilization -- Preclinical Experience with AMD3100 for Mobilization of Hematopoietic Stem and Progenitor Cells -- Molecular Pharmacology of CXCR4 Inhibition -- Combination Strategies for Stem Cell Mobilization Targeting CXCR4 and S1P Receptors -- Should We Stay or Should We Go Now? -- Cell Adhesion and Mobilization Pathways of Hematopoietic Cells -- Role of the CXCR4/CXCL12 Axis in Hematopoietic Stem Cell Trafficking -- Part 2. Clinical Studies on Stem Cell Mobilization -- Front matter -- Early Studies of AMD3100/Plerixafor in Healthy Volunteers -- The Current Role of Plerixafor in Stem Cell Mobilization for Hematopoietic Stem Cell Transplantation -- Experience with Apheresis Procedures After Plerixafor Mobilisation -- Influence of Different Stem Cell Mobilization Strategies on Graft Composition and Outcome of Autologous or Allogeneic Transplantation -- Relevance and Clinical Implications of Tumor Cell Mobilization in Autologous Transplantation of Multiple Myeloma and Non-Hodgkin's Lymphoma -- Plerixafor: Data from the Compassionate Use Program -- Stem Cell Mobilization with Chemomobilization Plus Plerixafor in Hard-to-Mobilize Patients: The Experience at Kuopio University Hospital, Finland -- Blood Stem Cell Mobilization in Solid Tumors: Experience with Plerixafor and Alternative Ways of Hematopoietic Stem Cell Mobilization -- Mobilization with Chemotherapy + G-CSF + Plerixafor in Europe: The Italian Experience -- Experience with Plerixafor in Poor Mobilizers -- CXCR4 Antagonists -- Part 3. CXCR4 Antagonists for Treatment of Leukemias and Solid Tumors -- Front matter -- Application of CXCR4 Inhibitors in Leukemia -- CXCR4 Antagonists for the Treatment of CML -- Targeting CXCR4 in Chronic Lymphocytic Leukemia: Preclinical Rationale and Early Clinical Experience -- CXCR4-Blockade for Treatment of Breast Cancer -- Molecular Imaging of CXCR4 Receptor Expression in Tumors -- Part 4. Novel applications -- Potential Use of CXCR4 Antagonists to Mobilize Endothelial and Mesenchymal Stem Cells -- Strategies to Enhance Hematopoietic Stem Cell Engraftment Following Transplantation -- Mobilization for Gene Therapy.
  • Digital
    Michael Robert Mancuso.
    Judah Folkman first hypothesized in 1971 that tumor growth depends on angiogenesis, the growth of new blood vessels from pre-existing ones. Eighteen years later, in 1989, Harold Dvorak and Napoleana Ferrara independently isolated Vascular Endothelial Growth Factor (VEGF), which Dr. Ferrara went on to prove to be one of the most important endothelial cell mitogens ever discovered. In 2004, the FDA approved the use of Bevacizumab, a monoclonal antibody against VEGF, for the treatment of colon cancer after the drug showed superiority when combined with a standard chemotherapy regimen. While the use of VEGF inhibitors in the clinic has shown benefit in treating patients, there is room for clinical improvement. This thesis was initially established on the concept that the identification of other novel regulators of angiogenesis could provide additional targets for anti-angiogenic therapy to be used either alone or in combination with VEGF inhibition to achieve results superior to VEGF blockade alone. Against this background, mouse models of conditional Egfl7, Egfl8, miR-126, and GPR124 genetic deletion were designed and generated to determine the function of these novel molecules in the context of regulating angiogenesis. We discovered that the previously described Egfl7 mouse knockout phenotypes of 50% embryonic lethality with edema and delayed cranial blood vessel formation and post-natal vascularization of the retina were actually due to disruption of the microRNA miR-126 residing in the seventh intron of Egfl7. We further showed that miR-126 regulates angiogenesis through suppressing expression of p85[beta] and Spred1 which inhibit the downstream signaling cascade of VEGF signaling. Surprisingly, the Egfl7 mice were phenotypically normal even with simultaneous deletion of the paralog Egfl8. We noted that the previous study inaccurately described the miR-126 angiogenic phenotypes as those of Egfl7 after using insertional deletion ("knockin") strategies to develop mouse models of Egfl7 gene deletion which inadvertently disrupted expression of miR-126. We highlight that this concept complicates the design of mouse genetic deletion studies and recommend that strategies which provide minimal deletion of a region of the locus are superior to "knockin" strategies. GPR124 is a known requirement of developmental angiogenesis specifically within the Central Nervous System (CNS). As GPR124 is expressed by endothelial cells and pericytes in the CNS, it was unknown at the start of my graduate career if endothelial cell or pericyte expression was required for blood vessel migration into the CNS. Using an endothelial cell specific driver, we were able to delete GPR124 specifically within endothelial cells and prove that endothelial cell expression of GPR124 drove vascularization of the CNS during development, thus proving that GPR124 functions endothelial cell autonomously. Further, as embryos lacking GPR124 die with 100% penetrance before birth, I crossed my conditional allele with an inducible driver to delete GPR124 in adulthood in order to bypass the window of embryonic lethality. I found that GPR124 was not required for homeostasis of the quiescent cerebrovasculature. Using orthotopic glioma and transient middle cerebral artery occlusion studies, we found that mice lacking GPR124 were susceptible to intracranial hemorrhage after insult. These results showed that GPR124 plays an important role in maintaining cerebrovacular integrity in the context of disease within the CNS. Overall, this thesis has identified, clarified or further described the functional role of several novel regulators of angiogenesis. Future studies beyond the scope of this thesis will use the mouse models of conditional gene deletion built for this dissertation to further investigate the mechanisms through which these genes regulate angiogenesis. Hopefully, further understanding of how each of these novel genes regulates endothelial cell physiology will provide valuable contributions towards improving out ability to treat cancer through manipulation of its blood supply.
  • Digital
    edited by Christophe Lavelle, Jean-Marc Victor.
    ScienceDirect2018
    Nuclear Architecture and Dynamics provides a definitive resource for (bio)physicists and molecular and cellular biologists whose research involves an understanding of the organization of the genome and the mechanisms of its proper reading, maintenance, and replication by the cell. This book brings together the biochemical and physical characteristics of genome organization, providing a relevant framework in which to interpret the control of gene expression and cell differentiation. It includes work from a group of international experts, including biologists, physicists, mathematicians, and bioinformaticians who have come together for a comprehensive presentation of the current developments in the nuclear dynamics and architecture field. The book provides the uninitiated with an entry point to a highly dynamic, but complex issue, and the expert with an opportunity to have a fresh look at the viewpoints advocated by researchers from different disciplines.
  • Digital
    edited by Shinichi Nakagawa, Tetsuro Hirose.
    Springer2015
    Visualization of lncRNA by single-molecule fluorescence in situ hybridization / Margaret Dunagin , Moran N. Cabili , John Rinn , and Arjun Raj -- Super-resolution imaging of nuclear bodies by STED microscopy / Yasushi Okada and Shinichi Nakagawa -- High-resolution 3D DNA FISH using plasmid probes and computational correction of optical aberrations to study chromatin structure at the sub-megabase scale / Luca Giorgetti, Tristan Piolot, and Edith Heard -- Time-lapse imaging of nuclear bodies / Saskia Hutten, Samuel Swift, and Angus I. Lamond -- Visualization of nucleic acids with synthetic exciton-controlled fluorescent oligonucleotide probes / Dan Ohtan Wang and Akimitsu Okamoto -- Live CLEM imaging to analyze nuclear structures at high resolution / Tokuko Haraguchi, Hiroko Osakada, and Takako Koujin -- Ultrastructural analysis of nuclear bodies using electron microscopy / Sylvie Souquere and Gérard Pierron -- Analyses of nuclear proteins and nucleic acid structures using atomic force microscopy / Jamie L. Gilmore [and eight others] -- Genome-wide co-localization screening of nuclear body components using a fluorescently tagged FLJ cDNA clone library / Tetsuro Hirose and Naoki Goshima -- Purification of specific chromatin regions using oligonucleotides : capture hybridization analysis of RNA targets (CHART) / Christopher P. Davis and Jason A. West -- RNA antisense purification (RAP) for mapping RNA interactions with chromatin / Jesse Engreitz, Eric S. Lander, and Mitchell Guttman -- In situ dissection of RNA functional subunits by domain-specific chromatin isolation by RNA purification (dChIRP) / Jeffrey J. Quinn and Howard Y. Chang -- Extracting, enriching, and identifying nuclear body sub-complexes using label-based quantitative mass spectrometry / Archa Fox [and three othrs] -- Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells : native ribonucleoprotein immunoprecipitation (RIP) assays / Joseph A. Cozzitorto [and eight others] -- Cross-linking and immunoprecipitation of nuclear RNA-binding proteins / Quan Li , Yuri Uemura , and Yukio Kawahara -- Purification of noncoding RNA and bound proteins using FLAG peptide-conjugated antisense-oligonucleotides / Shungo Adachi and Tohru Natsume -- MMCT-mediated chromosome engineering technique applicable to functional analysis of lncRNA and nuclear dynamics / Makiko Meguro-Horike and Shin-ichi Horike -- Reconstitution of nucleocytoplasmic transport using digitonin-permeabilized cells / Shingo Kose, Tomoko Funakoshi, and Naoko Imamoto -- Genome-wide analysis of long noncoding RNA turnover / Hidenori Tani [and eight others] -- Knockdown of nuclear-retained long noncoding RNAs using modified DNA antisense oligonucleotides / Xinying Zong [and six others] -- siRNA screening of nuclear proteins / Yuko Hasegawa and Shinichi Nakagawa.
  • Digital
    edited G.V. Shivashankar.
    ScienceDirect2010
    Section A. Cell Nucleus: Organization & MechanoBiology -- Chapter 1. Fluorescence Fluctuation Microscopy to Reveal 3D Architecture and Function in the Cell Nucleus / Thorsten Lenser, Klaus Weisshart, Tobias Ulbricht, Karolin Klement, Peter Hemmerich -- Chapter 2. Studying Histone Modifications and Their Genomic Functions by Employing Chromatin Immunoprecipitation and Immunoblotting / Ranveer S. Jayani, Praveena L. Ramanujam, Sanjeev Galande -- Chapter 3. Dynamic Organization of Chromatin Assembly and Transcription Factories in Living Cells / Bidisha Sinha, Dipanjan Bhattacharya, Deepak Kumar Sinha, Shefali Talwar, Shovamayee Maharana, Soumya Gupta, G.V. Shivashankar -- Chapter 4. Manipulation and Isolation of Single Cells and Nuclei / Swee Jin Tan, Qingsen Li, Chwee Teck Lim -- Chapter 5. Beyond Lamins: Other Structural Components of the Nucleoskeleton / Zhixia Zhong, Katherine L. Wilson, Kris Noel Dahl -- Chapter 6. Altered Mechanical Properties of the Nucleus in Disease / Maria Lucia Lombardi, Jan Lammerding -- Chapter 7. Theoretical Concepts and Models of Cellular Mechanosensing / Rumi De, Assaf Zemel, Samuel A. Safran -- Section B. Impact of Nuclear Mechanics on Function -- Chapter 8. Mechanical Induction of Gene Expression in Connective Tissue Cells / Matthew W.C. Chan, Boris Hinz, Christopher A. McCulloch -- Chapter 9. Physical Plasticity of the Nucleus and its Manipulation / Irena Ivanovska, Joe Swift, Takamasa Harada, J. David Pajerowski, Dennis E. Discher -- Chapter 10. Prestressed Nuclear Organization in Living Cells / Aprotim Mazumder, T. Roopa, Abhishek Kumar, K. Venkatesan Iyer, Nisha M. Ramdas, G.V. Shivashankar -- Chapter 11. Nanotopography/Mechanical Induction of Stem-Cell Differentiation / Benjamin Kim Kiat Teo, Soneela Ankam, Lesley Y. Chan, Evelyn K.F. Yim -- Chapter 12. Mechanical Induction in Embryonic Development and Tumor Growth: Integrative Cues Through Molecular to Multicellular Interplay and Evolutionary Perspectives / Maria-Elena Fernandez-Sanchez, Fanny Serman, Padra Ahmadi, Emmanuel Farge -- Chapter 13. Informatics-Based Analysis of Mechanosignaling in the Laminopathies / Frank P.L. Lai, Radfidah A. Mutalif, Siew Cheng Phua, Colin L. Stewart -- Chapter 14. Autosomal Dominant Leukodystrophy Caused by Lamin B1 Duplications: A Clinical and Molecular Case Study of Altered Nuclear Function and Disease / Quasar Saleem Padiath, Ying-Hui Fu.
  • Digital
    edited by Valérie Doyle.
    ScienceDirect2014
    Volume 122 of Methods in Cell Biology describes modern tools and techniques used to study nuclear pore complexes and nucleocytoplasmic transport in diverse eukaryotic model systems (including mammalian cells, Xenopus, C. elegans, yeast). The volume enables investigators to analyze nuclear pore complex structure, assembly, and dynamics; to evaluate protein and RNA trafficking through the nuclear envelope; and to design in vivo or in vitro assays appropriate to their research needs.
  • Digital
    edited by Iain J. McEwan.
    Springer2009
    Nuclear receptors : one big family / Iain J. McEwan -- Methods for measuring ligand dissociation and nuclear receptor turnover in whole cells / Elizabeth M. Wilson -- Flow cytometry as a tool for measurement of steroid hormone receptor protein expression in leukocytes / Cherie L. Butts and Esther M. Sternberg -- X-ray crystallography of agonis/antagonist-bound receptors / Ashley C.W. Pike -- FRAP and FRET methods to study nuclear receptors in living cells / Martin E. van Royen ... [et al.] -- Receptor-DNA interactions : EMSA and footprinting / Jason T. Read ... [et al.] -- Chromatin immunoprecipitation (ChIP) methodology and readouts / Charles E. Massie and Ian G. Mills -- Yeast-based reporter assays for the functional characterization of cochaperone interactions with steroid hormone receptors / Heather A. Balsiger and Marc B. Cox -- High throughput analysis of nuclear receptor-cofactor interactions / Michael L. Goodson, Behnom Farboud, and Martin L. Privalsky -- Binding affinity and kinetic analysis of nuclear receptor/co-regulator interactions using surface plasmon resonance / Derek N. Lavery -- Using RNA interference to study protein function / Carol D. Curtis and Ann M. Nardulli -- Using intrinsic fluorescence emission spectroscopy to study steroid receptor and coactivator protein conformation dynamics / Kate Watt and Iain J. McEwan -- Development of phosphorylation site-specific antibodies to nuclear receptors / Inés Pineda Torra ... [et al.] -- Tissue-selective knockouts of steroid receptors : a novel paradigm in the study of steroid action / Karel De Gendt and Guido Verhoeven -- Methods for identifying and studying genetic alterations in hormone-dependent cancers / Outi R. Saramäki, Kati K. Waltering, and Tapio Visakorpi.
  • Digital
    edited by Chris M. Bunce, Moray J. Campbell.
    Springer2010
    Nuclear receptors an introductory overview / C.M. Bunce and M.J. Campbell -- What does evolution teach us about nuclear receptors? / G. Markov, F. Bonneton and V. Laudet -- Functions of nuclear receptors in insect development / D. Martín -- The glucocorticoid receptor / R.H. Oakley and J.A. Cidlowski -- Estrogen receptors: Their actions and functional roles in health and disease / S. Nilsson and J.-Å. Gustafsson -- Androgen receptor / J.T. Dalton and W. Gao -- Thyroid hormone receptors / B. Vennström, H. Liu and D. Forrest -- The vitamin D receptor (NR1I1) / O. Maguire and M.J. Campbell -- Retinoic acid receptors / A. Cras, F. Guidez and C. Chomienne -- PPARs: Important regulators in metabolism and inflammation / L.M. Sanderson and S. Kersten -- Xenobiotic receptors CAR and PXR / C. Klaassen and H. Lu -- Fxr / Y. Wang ... [et al.] -- Physiological functions of TR2 and TR4 orphan nuclear receptor / S. Liu ... [et al.] -- Nuclear receptors and ATP dependent chromatin remodeling: A complex story / C.J. Burd and T.K. Archer -- Non-genomic action of sex steroid hormones / A. Migliaccio, G. Castoria and F. Auricchio -- Ligand regulation and nuclear receptor action / M. Hewison -- New insights to nuclear receptor gene regulation from analysis of their response elements in target genes / C. Carlberg -- Systems biology: Towards realistic and useful models of molecular networks / F.J. Bruggeman ... [et al.].
  • Digital
    edited by Nathalie Beaujean, Hélène Jammes, Alice Jouneau.
    Springer2015
    Nuclear Transfer in the Mouse -- Nuclear Transfer in the Rabbit -- Nuclear Transfer in Ruminants -- Nuclear Transfer and Transgenesis in the Pig -- Embryonic Stem Cell-Somatic Cell Fusion and Post-Fusion Enucleation -- Analysis of Nuclear Reprogramming Following Nuclear Transfer to Xenopus Oocyte -- Assessing the Quality of Donor Cells: Karyotyping Methods -- Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer -- Fluorescent Immunodetection of Epigenetic Modifications on Pre-Implantation Mouse Embryos -- Visualization of Epigenetic Modifications in Preimplantation Embryos -- Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer -- Analysis of Nucleolar Morphology and Protein Localization as an Indicator of Nuclear Reprogramming -- Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining -- Gene Expression Analysis in Early Embryos through Reverse Transcription Quantitative PCR (RT-qPCR) -- Studying Bovine Early Embryo Transcriptome by Microarray -- Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level -- Micro Chromatin Immunoprecipitation ({uF06D}ChIP) from Early Mammalian Embryos -- Assessing Reprogramming by Chimera Formation and Tetraploid Complementation -- Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology -- Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP).
  • Digital
    Justin Ainscough, Shinya Yamanaka, Takashi Tada, editors.
    Springer2011
    Annotation Research into the field of stem cell biology has developed exponentially over recent years, and is beginning to offer significant promise for unravelling the molecular basis of a multitude of disease states. Importantly, in addition to offering the opportunity to delve deeply into the mechanisms that drive disease aetiology the research is realistically opening the doors for development of targeted and personalized therapeutic applications that many considered, until recently, to be nothing more that a far fetched dream. This volume provides a timely glimpse into the methods that have been developed to instigate, and the mechanisms that have been identified to drive, the process of nuclear reprogramming, chronicling how the field has developed over the last 50-60 years. Since the early 1950s a small number of notable experiments have provided significant impetus to the field, primarily the demonstration of reprogramming ability, first by the complex cytoplasmic milieu that constitutes the amphibian egg, then that of the mammalian egg, and finally that of the mammalian embryonic stem cell. Most recently, the demonstration that a limited pool of defined molecules is capable of reprogramming a multitude of cell types has provided massive impetus and facilitated transition towards realistic therapeutic application. We have therefore reproduced some of the key articles that elegantly document these dramatic stages of development of the field in an inclusive appendix to the book, for the benefit of readers keen to investigate the history of how the field of stem cell biology has evolved. Owing to the ever broadening nature of this field, and the incredible rate at which it is evolving, the main content of this volume focuses on areas that have shown significant movement in recent years, are most likely to translate into personalized therapeutic application, and thus provide greatest potential for significant impact on human health in the not too distant future. We recognize that research into many other disease states and cell types are all equally worthy of discussion. We would therefore like to acknowledge those researchers involved whose work we have not been able to include in this volume. Nuclear Reprogramming and Stem Cells will serve as a valuable resource for all researchers in the field of stem cell biology, including those just setting out on their career path as well as those already established in the field.
  • Digital
    Jørgen Kjems, Elena Ferapontova, Kurt V. Gothelf, editors.
    Springer2014
    This volume on nucleic acid nanotechnology offers authoritative, up-to-date and comprehensive coverage of nanotechnological studies and applications of nucleic acids. It provides reviews of various aspects of nucleic acid nanotechnology, each written by an internationally leading expert in the field, and presents state-of-the-art and recent advances in nucleic acid synthetic modifications, nanoscale design, manipulation and current and future applications in bioengineering, medicine, electronics, genetic analysis, chemistry, molecular biology, surface and material sciences. It examines how nucleic acid research is merging with nanotechnology, allowing the nanoscale properties of nucleic acid to be exploited in performing challenging nanotechnological tasks, from nanorobotics and nanosensing to nucleic acid computing. This book will above all benefit anyone who is interested in nanotechnological concepts of nucleic acid design and applications, and offers a valuable resource for teaching these concepts. It is essential reading for a broad audience of scientists both in academia and industry who wish to expand their expertise on the potential of nucleic acid functions and applications.
  • Digital
    Katsuhiko S. Murakami, Michael A. Trakselis, editors.
    Springer2014
    This book provides a review of the multitude of nucleic acid polymerases, including DNA and RNA polymerases from Archea, Bacteria and Eukaryota, mitochondrial and viral polymerases, and other specialized polymerases such as telomerase, template-independent terminal nucleotidyl transferase and RNA self-replication ribozyme. Although many books cover several different types of polymerases, no book so far has attempted to catalog all nucleic acid polymerases. The goal of this book is to be the top reference work for postgraduate students, postdocs, and principle investigators who study polymerase.
  • Digital
    Mark O.J. Olson [editor].
    Springer2011
  • Digital
    edited by Ronald Hancock.
    Springer2015
    Cell type-specific affinity purification of nuclei for chromatin profiling in whole animals / Florian A. Steiner and Steven Henikoff -- Lysis gradient centrifugation : a flexible method for the isolation of nuclei from primary cells / Karl Katholnig ... [et al.] -- Isolation of nuclei in media containing an inert polymer to mimic the crowded cytoplasm / Ronald Hancock and Yasmina Hadj-Sahraoui -- New rapid method for isolating nucleoli / Zhou Fang Li and Yun Wah Lam -- Sequential recovery of macromolecular components of the nucleolus / Baoyan Bai and Marikki Laiho -- Au nanoinjectors for electrotriggered gene delivery into the cell nucleus / Mijeong Kang and Bongsoo Kim -- Improving chromatin immunoprecipitation (ChIP) by suppression of method-induced DNA-damage signaling / Sascha Beneke -- Purification of specific chromatin loci for proteomic analysis / Stephanie D. Byrum, Sean D. Taverna, and Alan J. Tackett -- Chromatin structure analysis of single gene molecules by psoralen cross-linking and electron microscopy / Christopher R. Brown ... [et al.] -- Purification of proteins on newly synthesized DNA using iPOND / Huzefa Dungrawala and David Cortez -- Applying the ribopuromycylation method to detect nuclear translation / Alexandre David and Jonathan W. Yewdell -- Targeted nano analysis of water and ions in the nucleus using cryo-correlative microscopy / Frédérique Nolin ... [et al.] -- Redox-sensitive yellow fluorescent protein sensor for monitoring nuclear glutathione redox dynamics / Agata Banach-Latapy, Michèle Dardalhon, and Meng-Er Huang -- Determination of the dissociation constant of the NF[Kappa]B p50/p65 heterodimer in living cells using fluorescence cross-correlation spectroscopy / Manisha Tiwari and Masataka Kinjo -- Imaging and quantification of amyloid fibrillation in the cell nucleus / Florian Arnhold, Andrea Scharf, and Anna von Mikecz -- Analysis of nuclear organization with TANGO, software for high-throughput quantitative analysis of 3D fluorescence microscopy images / Jean Ollion ... [et al.] -- Quantitative analysis of chromosome localization in the nucleus / Sandeep Chakraborty ... [et al.].
  • Digital
    edited by Chrissa Kioussi.
    Springer2012
    Histological analysis of the embryonic and adult tooth / Atsushi Ohazama -- Determination of gene expression patterns by whole-mount in situ hybridization / Sergiy Kyryachenko [and others] -- Determination of gene expression patterns by in situ hybridization in sections/ Kateryna Kyrylkova [and others] -- Immunohistochemistry and detection of proliferating cells by BrdU / Sergiy Kyryachenko [and others] -- Detection of apoptosis by TUNEL assay / Kateryna Kyrylkova [and others] -- Use of siRNA in dental tissue-derived cell cultures : integrin knockdown in fibroblasts / Malgorzata M. Barczyk, Donald Gullberg, and Anne Isine Bolstad -- Organ cultures and kidney-capsule grafting of tooth germs / Keishi Otsu, Naoki Fujiwara, and Hidemitsu Harada -- Evaluation of skull and tooth morphology and mineralization using high-resolution x-ray tomography / Brian K. Bay -- Electron microscopy / Hans U. Luder and Margrit Amstad-Jossi -- Deoxyoligonucleotide microarrays for gene expression profiling in murine tooth germs / Harald Osmundsen, Anne-Marthe Jevnaker, and Maria A. Landin -- Lineage differentiation of mesenchymal stem cells from dental pulp, apical papilla, and periodontal ligament / Kentaro Akiyama [and others] -- In vivo transplantation and tooth repair / Shuhei Tsuchiya and Masaki J. Honda -- Methods to validate tooth-supporting regenerative therapies / Miguel Padial-Molina [and others] -- Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (organ germ method) / Masamitsu Oshima [and others] -- In vitro studies on odontogenic tumors / Javier Caton, Thimios A. Mitsiadis, and Peter R. Morgan -- Whole mount immunohistochemistry and in situ hybridization of larval and adultzZebra fish dental tissues / Barbara Verstraeten, Ellen Sanders, and Ann Huysseune.
  • Digital
    edited by Mikhail A. Nikiforov.
    Springer2017
  • Digital/Print
    S. Samuel Kim, editor.
    Digital : Springer2013
    Print2013
    Fertility preservation has become one of the most important and fast growing fields of reproductive medicine. Although there are several strategies for fertility preservation in female, most of them are still considered experimental. It is important to perfect the existing technologies, but also developing new strategies should be actively sought. The future development of fertility preservation strategies should be based on the sound scientific knowledge and principles. One of the main objectives of fertility preservation in females is prevention of oocyte depletion. The mechanisms of oocyte loss and survival in the ovary are complex, which include genetic control both in germ cells and in somatic cells, DNA damage and repair mechanism, apoptosis and autophagy, and other poorly understood molecular mechanisms.To develop clinically effective and safe strategies for fertility preservation, it is essential to know and understand the fundamentals of oocyte and ovarian biology at the molecular level. Thus, the purpose of this edition is to review the current progress in research related to molecular and genetic control of oocyte development that can be applied to fertility preservation. The main topics that are discussed in this publication include molecular signaling mechanisms of oocyte activation and loss, genomic integrity of oocytes, and epigenetics.
  • Print
    Malgorzata Kloc, editor.
    1. Exoogenous molecule and organelle delivery in oogenesis -- 2. Control of mammalian oocyte development by interactions with the matenal follicular environment -- 3. Transovarial transmission of symbionts in insects -- 4. Acquisition of oocyte polarity -- 5. The pole (germ) plasm in insect oocytes -- 6. Multiple functions of the DEAD-Box helicase vasa in Drosophila oogenesis -- 7. The role of microtubule motors in mRNA localization and patterning within the Drosophila oocyte -- 8. Phosphoinositides and cell polarity in the Drosophila egg chamber -- 9. RNA localization in the vertebrate oocyte: establishment of oocyte polarity and localized mRNA assemblages -- 10. DNA methyltransferases in mammalian oocytes -- 11. Accumulation of chromatin remodelling enzyme and histone transcripts in bovine oocytes -- 12. Translational regulation in the mammalian oocyte -- 13. Regulation of translationally repressed mRNAs in zebrafish and mouse oocytes -- 14. Switches in dicer activity during oogenesis and early development -- 15. The regulation and function of cohesin and condensin in mammalian oocytes and spermatocytes -- 16. Supply and demand of energy in the oocyte and the role of mitochondria -- 17. Functions of vitellogenin in eggs -- 18. Lipids in insect oocytes: from the storage pathways to their multiple functions -- 19. Parthenogenesis in insects: the centriole renaissance -- 20. The origin and evolution of maternal genes -- 21. Noninheritable maternal factors useful for genetic manipulation in mammals.
  • Digital
    Giovanni Coticchio, David F. Albertini, Lucia De Santis, editors.
    Springer2013
    The oocyte is the largest and most mysterious cell in the body of mammalian organisms. Through its growth and maturation, it reaches extreme levels of specialization, while maintaining at the same time a condition of totipotency. Its unique ability, in co-operation with the spermatozoon, to give rise to a fully developed organism formed from hundreds of different tissues and myriads of individual cells has inspired intellectuals of all ages.Oogenesis finds impetus and purpose in casting scientific perspective towards this unique cell for the benefit of scientists and assisted reproductive technology (ART) specialists. The authors of the chapters are distinguished authorities in their respective areas of competence. From the opening of the book the reader is lead on a fantastic voyage from the formation of the primordial oocyte to the development of the early embryo, passing through crucial processes of oogenesis, such as co-ordination of oocyte and follicle growth, gene expression and organelle reorganization during growth and maturation, epigenetic mechanisms, regulation of meiosis, totipotency, cell polarity, oogenesis in vitro and maternal regulation of early development.
  • Digital
    Alberto Diaspro, editor.
    Springer2011
    Fundamentals of optical microscopy / Franco Quercioli -- The white confocal: continuous spectral tuning in excitation and emission / Rolf Borlinghaus -- Second/third harmonic generation microscopy / Shakil Rehman ... [et al.] -- Role of scattering and nonlinear effects in the illumination and the photobleaching distribution profiles / Zeno Lavagnino, Francesca Cella Zanacchi and Alberto Diaspro -- New analytical tools for evaluation of spherical aberration in optical microscopy / Isabel Escobar ... [et al.] -- Improving image formation by pushing the signal-to-noise ratio / Emiliano Ronzitti ... [et al.] -- Site-specific labeling of proteins in living cells using synthetic fluorescent dyes / Gertrude Bunt -- Imaging molecular physiology in cells using FRET-based fluorescent nanosensors / Fred S. Wouters -- Measuring molecular dynamics by FRAP, FCS, and SPT / Kevin Braeckmans ... [et al.] -- In vitro-in vivo fluctuation spectroscopies / M. Collini ... [et al.] -- Interference x-ray diffraction from single muscle cells reveals the molecular basis of muscle braking / L. Fusi ... [et al.] -- Low concentration protein detection using novel SERS devices / Gobind Das ... [et al.] -- Near infrared three-dimensional nonlinear optical monitoring of stem cell differentiation / Uday K. Tirlapur and Clarence Yapp -- A correlative microscopy: a combination of light and electron microscopy / Umberto Fascio and Anna Sartori-Rupp.
  • Digital
    Robert Barretto.
    Optical microendoscopy is an emerging modality for imaging in live subjects. Using gradient refractive index (GRIN) microlenses, microendoscopy enables subcellular-resolution imaging in deep tissues that are inaccessible by traditional imaging techniques. We present a platform of methods and technologies that build upon GRIN microendoscopy: 1) miniaturized microscopes for imaging in awake, behaving animals, 2) methods for imaging contractile dynamics in the muscles of animal and human subjects, 3) chronic brain preparations that allow for longitudinal examinations of subcellular neuronal features and disease progression, and 4) novel microendoscope probes whose imaging capabilities approach that of standard water-immersion microscope objectives. When combined with the broad sets of available fluorescent reporters, and minimally invasive surgical preparations, the work described in this dissertation enables sophisticated experimental designs for probing how cellular characteristics may underlie or explain behavior, in models of both healthy and diseased states.
  • Digital
    Ravi Mehrotra, editor.
    Springer2013
    Oral Cytology: A Concise Guide provides a state-of-the-art review of the major issues specific to the field of oral cytology. The sections of the book have been structured to review the complexities of sample collection, manual/automated analysis, pitfalls, gray zones and controversies in diagnosis, as well as, issues not covered by other available materials. Recent advancements in ancillary techniques like DNA ploidy, image analysis and molecular biology are discussed in detail. This useful text is extensively illustrated with patient and microscopic pictures, which will further highlight the clinical application. Throughout the text, simultaneous inclusion of both cytologic and histologic images will allow cyto-histological correlation. Readers will find descriptions of techniques offering greater simplicity, speed, patient comfort and cost effectiveness, as well as improved diagnostic precision. This bench-side reference is a unique and valuable resource in the field of oral cytology, both for those currently in training and clinicians dealing with the diagnosis of oral lesions.
  • Digital
    [edited by] Svante R. Orell, Gregory F. Sterrett.
    ScienceDirect2012
    ClinicalKey2012
    Chapter 1 Introduction -- Chapter 2 The techniques of FNA cytology -- Chapter 3 Imaging methods for guidance of aspiration cytology -- Chapter 4 Head and neck; salivary glands -- Chapter 5 Lymph nodes -- Chapter 6 The thyroid gland -- Chapter 7 Breast -- Chapter 8 Lung, chest wall and pleura -- Chapter 9 Mediastinum -- Chapter 10 Liver and spleen -- Chapter 11 Pancreas, biliary tract and intra-abdominal organs -- Chapter 12 Kidney, adrenal and retroperitoneum -- Chapter 13 Male and female genital organs -- Chapter 14 Skin and subcutis -- Chapter 15 Soft tissues -- Chapter 16 Bone and cartilage -- Chapter 17 Paediatric tumors -- Chapter 18 Infectious Diseases.
  • Digital
    edited by Takashi Tsuji.
    Springer2017
    Generation of various telencephalic regions from human embryonic stem cells in three-dimensional culture / Taisuke Kadoshima, Hideya Sakaguchi, and Mototsugu Eiraku -- Generation of a three-dimensional retinal tissue from self-organizing human ESC culture / Atsushi Kuwahara, Tokushige Nakano, and Mototsugu Eiraku -- 3D culture for self-formation of the cerebellum from human pluripotent stem cells through Induction of the isthmic organizer / Keiko Muguruma -- Reconstitution of a patterned neural tube from single mouse embryonic stem cells / Keisuke Ishihara, Adrian Ranga, Matthias P. Lutolf, Elly M. Tanaka, and Andrea Meinhardt -- Functional pituitary tissue formation / Chikafumi Ozone and Hidetaka Suga -- Directed differentiation of mouse embryonic stem cells into inner ear sensory epithelia in 3D culture / Jing Nie, Karl R. Koehler, and Eri Hashino -- Generation of functional thyroid tissue using 3D-based culture of embryonic stem cells / Francesco Antonica, Dominika Figini Kasprzyk, Andrea Alex Schiavo, Mírian Romitti, and Sabine Costagliola -- Functional tooth regeneration / Masamitsu Oshima, Miho Ogawa, and Takashi Tsuji -- Functional hair follicle regeneration by the rearrangement of stem cells / Kyosuke Asakawa, Koh-ei Toyoshima, and Takashi Tsuji -- Functional salivary gland regeneration / Miho Ogawa and Takashi Tsuji -- Generation of a bioengineered lacrimal gland by using the organ germ method / Masatoshi Hirayama, Kazuo Tsubota, and Takashi Tsuji -- Generation of gastrointestinal organoids from human pluripotent stem cells / Jorge O. Múnera and James M. Wells -- Generation of a three-dimensional kidney structure from pluripotent stem cells / Yasuhiro Yoshimura, Atsuhiro Taguchi, and Ryuichi Nishinakamura -- Making a kidney organoid using the directed differentiation of human pluripotent stem cells / Minoru Takasato and Melissa H. Little -- Liver regeneration using cultured liver bud / Keisuke Sekine, Takanori Takebe, and Hideki Taniguchi -- Formation of stomach tissue by organoid culture using mouse embryonic stem cells / Taka-aki K. Noguchi and Akira Kurisaki -- In vivo model of small intestine / Mahe M. Maxime, Nicole E. Brown, Holly M. Poling, and Helmrath A. Michael.
  • Digital
    Mitsuo Tagaya, Thomas Simmen, editors.
    Springer2017
    "This book provides the first comprehensive coverage of the quickly evolving research field of membrane contact sites (MCS). A total of 16 chapters explain their organization and role and unveil the significance of MCS for various diseases. MCS, the intracellular structures where organellar membranes come in close contact with one another, mediate the exchange of proteins, lipids, and ions. Via these functions, MCS are critical for the survival and the growth of the cell. Owing to that central role in the functioning of cells, MCS dysfunctions lead to important defects of human physiology, influence viral and bacterial infection, and cause disease such as inflammation, type II diabetes, neurodegenerative disorders, and cancer. To approach such a multifaceted topic, this volume assembles a series of chapters dealing with the full array of research about MCS and their respective roles for diseases. Most chapters also introduce the history and the state of the art of MCS research, which will initiate discussion points for the respective types of MCS for years to come. This work will appeal to all cell biologists as well as researchers on diseases that are impacted by MCS dysfunction. Additionally, it will stimulate graduate students and postdocs who will energize, drive, and develop the research field in the near future."--Publisher's description.
  • Digital
    Daniel James Dickinson.
    A fundamental characteristic of metazoans is the formation of a simple, polarized epithelium. In higher animals, the structural integrity and functional polarization of simple epithelia require a cell-cell adhesion complex containing a classical cadherin, the Wnt-signaling protein [Beta]-catenin and the actin-binding protein [Alpha]-catenin. I have investigated the evolutionary origins of epithelial cell polarity and of the cadherin-catenin complex. I show that the non-metazoan Dictyostelium discoideum forms a polarized epithelium that is essential for multicellular development. Although D. discoideum lacks a cadherin homolog, I have identified and characterized an [Alpha]-catenin ortholog that binds a [Beta]-catenin-related protein. Both proteins are essential for formation of the epithelium, polarized protein secretion and proper multicellular morphogenesis. Thus the organizational principles of metazoan multicellularity may be more ancient than previously recognized, and the role of the catenins in cell polarity predates the evolution of Wnt signaling and classical cadherins.
  • Digital
    Hubert Wartenberg, Andreas Miething, Kjeld Møllgård.
    Springer2019
  • Digital/Print
    Olivier Civelli, Qun-Yong Zhou (eds.).
    Digital : Springer2006
    Print2008
  • Digital/Print
    edited by Dieter Häussinger, Helmut Sies.
    Digital : ScienceDirect2007
    Print2007
    Water compartments in cells / G. D. Fullerton ... [et al.] -- Yeast osmoregulation / S. Hohmann ... [et al.] -- Physiological analysis of bacterial mechanosensitive channels / I. R. Booth ... [et al.] -- Control of cell cycle in response to osmostress: Lessons from yeast / J. Clotet ... [et al.] -- Bacterial osmosensing transporters / J. M. Wood -- Plant gene networks in osmotic stress response: From genes to regulatory networks / L. S. Tran ... [et al.] -- Osmosensing by integrins in rat liver / F. Schliess ... [et al.] -- Hyperosmotic activation of the CD95 system / R. Reinehr ... [et al.] -- New approaches for determining apoptotic volume decrease in cells / C. D. Bortner ... [et al.] -- Transient receptor potential channels in mechanosensing and cell volume regulation / S. F. Pedersen ... [et al.] -- Cell volume regulatory ion channels in cell proliferation and cell death / F. Lang ... [et al.] -- Actin cytoskeleton architecture and signaling in osmosensing / E. A. Papakonstanti ... [et al.] -- Osmotic stress and DNA damage / N. I. Dmitrieva ... [et al.] -- Transcriptional activator tone-binding protein in cellular protection and differentiation / J. A. Kim ... [et al.] -- Desiccation response of mammalian cells: Anhydrosignaling / Z. Huang ... [et al.] -- Tonicity-regulated gene expression / J. D. Ferraris ... [et al.] -- Hyperosmotic induction of mitogen-activated protein kinase scaffolding / T. L. Hilder ... [et al.] -- Osmoregulation of bile formation / R. Kubitz ... [et al.] -- Osmosignaling and volume regulation in intestinal epithelial cells / C. H. Lim ... [et al.] -- Osmotic regulation of cellular glucose uptake / P. Gual ... [et al.] -- Effects of osmolytes on protein folding and aggregation in cells / Z. Ignatova ... [et al.] -- Simulations of macromolecules in protective and denaturing osmolytes: Properties of mixed solvent systems and their effects on water and protein structure and dynamics / D. A. Beck ... [et al.] -- Application of the transfer model to understand how naturally occurring osmolytes affect protein stability / M. Auton ... [et al.] -- Mechanisms of high salinity tolerance in plants / N. Tuteja -- Phenotype of the taurine transporter knockout mouse / U. Warskulat ... [et al.] -- Molecular basis of osmolyte effects on protein and metabolites / J. Rosgen -- Methods of changing biopolymer volume fraction and cytoplasmic solute concentrations for in vivo biophysical studies / M. C. Konopka ... [et al.] -- Characterization of plant aquaporins / R. Kaldenhoff ... [et al.].
  • Digital
    edited by Tahira Farooqui, Akhlaq A. Farooqui.
    Wiley2012
  • Digital
    Scott Fraser Owen.
    Neural circuits throughout the brain are under the continuous influence of neuromodulators which shape network activity in accordance with behavioral context. Oxytocin is a key neuromodulator that has been linked to social memory and maternal behavior in animals, as well as to autism spectrum disorders, trust, emotion recognition and parenting in humans. Here we show that activation of oxytocin receptors sharpens the responses of the hippocampal circuit, increasing the signal of spike transmission through the network while simultaneously suppressing the noise of background spontaneous activity. Both of these actions are mediated through a depolarization of the fast-spiking interneurons. The resulting increase in inhibitory tone serves to silence spontaneous activity in the CA1 pyramidal cells, while a use-dependent depression of the inhibitory synapses permits enhanced feed-forward spike transmission. Furthermore, we show that oxytocin potently modulates spontaneous hippocampal Sharp-Wave Ripple oscillations in a slice preparation. These results elucidate the action of oxytocin in the hippocampus, while simultaneously shedding light on a novel mechanism by which modulation of fast-spiking interneurons can modify hippocampal circuit activity.
  • Digital
    Colleen Ann Brady.
    The p53 protein suppresses tumorigenesis by inducing cell cycle arrest, apoptosis, or cellular senescence. At the molecular level p53 plays a central role as a transcriptional activator, and p53 has two amino-terminal transactivation domains important for this role. However, the contribution of each of these domains to p53 activity has not been determined in vivo. To analyze transactivation domain requirements for p53 function, we have generated a series of p53 knock-in mouse models in which we replaced the endogenous p53 gene with transactivation mutants containing point mutations in the first (p53^25,26), second (p53^53,54), or both (p53^25,26,53,54) p53 transactivation domains. These experiments are the first to address the unique contribution of each of the p53 transactivation domains in vivo. By gene expression microarray, we determined that these mutants represent an allelic series of p53 transactivation mutants, whereby p53^53,54 retains wild-type p53 transactivation potential, p53^25,26,53,54 loses nearly all p53-dependent transactivation, and p53^25,26 exhibits a severely compromised transactivation phenotype. Experiments comparing all three transactivation domain mutants in cell culture and mouse models have unveiled important differences in the function of the two transactivation domains. Apoptosis or cell-cycle arrest downstream of acute DNA damage rely on the first transactivation domain and robust p53 transactivation, while senescence and tumor suppression in response to oncogene activation can occur without the full transactivation function of p53. These results implicate unique p53 transcriptional programs in acute DNA damage responses and tumor suppression, and mechanistically distinguish these two p53 response pathways. Further, we have uncovered striking differences in the developmental phenotypes of these different mutant mice, leading to conclusions about the activity of p53 tetramers.
  • Digital/Print
    edited by Miriam Ramírez-Domínguez.
    Digital : Springer2016
    Print2016
    Historical Background of Pancreatic Islet Isolation / Miriam Ramírez-Domínguez -- The Different Faces of the Pancreatic Islets / Midhat H.Abdulreda, Rayner Rodriguez-Diaz, Over Cabrera, Alejandro Caicedo, Per-Olof Berggren -- Isolation of Mouse Pancreatic Islets of Langerhans / Miriam Ramírez-Domínguez -- Pancreatic Islets: Methods for Isolation and Purification of Juvenile and Adult Pig Islets / Heide Brandhorst, Paul R.V. Johnson, Daniel Brandhorst -- Isolation of Pancreatic Islets from NonHuman Primates / Dora M. Berman -- Necessities for a Clinical Islet Program / Wayne John Hawthorne -- Clinical Islet Isolation / Wayne John Hawthorne, Lindy Jane Williams, Yi Vee Chew -- Index.
  • Digital
    Juan Domínguez-Bendala.
    Springer2009
  • Digital
    Hou, Jianghui.
    ScienceDirect2019
    The Paracellular Channel: Biology, Physiology and Disease serves as the first volume to offer a cohesive and unifying picture of the critical functions of paracellular channels (tight junctions) in different tissues. This new class of ion channel utilizes a completely different mechanism to create ion passage pathways across the cell junction. This volume outlines common principles that govern the organization and regulation of these diverse cellular structures, describes the methodology of study, and highlights the pathophysiologic consequence of abnormal structure and functions of the paracellular channels in human diseases. Coverage includes biochemical, biophysical, structural, physiologic analyses of the paracellular channel, and new technologies for recording and characterization.
  • Digital
    Samuel Bandara.
    Cell signaling networks are complex dynamical systems with mechanisms for adaptation and failure-tolerance such that typical stimulus experiments are often robust to the perturbation of individual components. While such robustness is often advantageous, it also means that the information content of an experiment with respect to internal states and activities is often poor. We treat this problem formally by examining the statistical uncertainty of parameter estimates of corresponding signaling models as a function of experimental protocols that are based on live-cell imaging of fluorescent reporters. The first part of this work uses such model predictions of parameter sensitivity to design numerically optimized live-cell imaging protocols with the goal to maximally constrain the space of possible parameter values. The second part instead uses an experimental approach to define invariant parameters by correlated population estimates in order to reveal changes of regulated parameters in systematic siRNA perturbation experiments. Our results show that the effects of siRNA perturbations on reporter dynamics can be used to infer functional changes of the probed system. We exploit this feature to define not only the key molecules responsible for activity but also direct and indirect regulators.
  • Digital
    Dorothy L. Rosenthal, Eva M. Wojcik, Daniel F.I. Kurtycz, editors.
    Springer2016
    Pathogenesis of Urothelial Carcinoma -- Adequacy of Urine Specimens (Adequacy) -- Negative for High Grade Urothelial Carcinoma (Negative) -- Atypical Urothelial Cells (AUC) -- Suspicious for High Grade Urothelial Carcinoma (Suspicious) -- High Grade Urothelial Carcinoma (HGUC) -- Low Grade Urothelial Neoplasia (LGUN) -- Other Malignancies Primary and Metastatic and Miscellaneous Lesions -- Ancillary Studies in Urinary Cytology -- Cytopreparatory Techniques -- Clinical Management, Including Microscopic Hematuria.
  • Digital/Print
    Ellen Hsu, Louis Du Pasquier.
    Digital : Springer2015
    Print2015
    Evolution of immunity and pathogens -- A host-pathogen interaction reduced to first principles: Antigenic variation in T. brucei -- Antigenic variation in Plasmodium falciparum -- Polymorphic mucin-like proteins in Schistosoma mansoni, a variable antigen and a key component of the compatibility between the schistosome and its snail host -- Fibrinogen-related proteins (FREP) in mollusks -- Somatic and germline diversification of a putative immunoreceptor within one phylum: Dscam in arthropods -- An immune effector system in the Protochordate gut sheds light on fundamental aspects of vertebrate immunity -- Variable lymphocyte receptors: a current view -- Antibody repertoire in fish -- Unique features of fish immune repertoires: particularities of the adaptive immunity within the largest group of vertebrates -- The evolution and structure of atypical T cell receptors -- Diversification of the primary antibody repertoire by AID-mediated gene conversion -- Antibody isotype switching in vertebrates.
  • Digital
    Christopher E. Dandoy, Joanne M. Hilden, Amy L. Billett, Brigitta U. Mueller, editors.
    Springer2017
  • Digital
    edited by Andras Nagy, Kursad Turksen.
    Springer2016
    Generation of patient-specific induced pluripotent stem cell from peripheral blood mononuclear cells by Sendai reprogramming vectors / Oscar Quintana-Bustamante and Jose C. Segovia -- Doxycycline-inducible system for genetic correction of iPSC disease models / Xiuli Sim ... [et al.] -- Generation and characterization of patient-specific induced pluripotent stem cell for disease modeling / Renuka Sivapatham and Xianmin Zeng -- Modeling genomic imprinting disorders using induced pluripotent stem cells / Stormy J. Chamberlain ... [et al.] -- Generation and characterization of induced pluripotent stem cells from patients with mtDNA mutations / Riikka H. Hssamssalssainen and Anu Suomalainen -- Skin biopsy and patient-specific stem cell lines / Yao Li, Huy V. Nguyen, and Stephen H. Tsang -- Directed myogenic differentiation of human induced pluripotent stem cells / Emi Shoji, Knut Woltjen, and Hidetoshi Sakurai -- Using human induced pluripotent stem cells to model skeletal diseases / Emilie Barruet and Edward C. Hsiao -- Modeling cardiovascular diseases with patient-specific human pluripotent stem cell-derived cardiomyocytes / Paul W. Burridge ... [et al.] -- Calcium imaging in pluripotent stem cell-derived cardiac myocytes / Anna Walter ... [et al.] -- Patient-specific induced pluripotent stem cell models : generation and characterization of cardiac cells / Fabian Zanella and Farah Sheikh -- Differentiation of human pluripotent stem cells to cardiomyocytes under defined conditions / Cathelijne W. van den Berg ... [et al.] -- Generation of cardiomyocytes from pluripotent stem cells / Hiroko Nakahama and Elisa Di Pasquale -- Generation and characterization of patient-specific iPSC model for cardiovascular disease / Yee Ki Lee ... [et al.] -- Transgene-free disease-specific iPSC generation from fibroblasts and peripheral blood mononuclear cells / Kerem Fidan ... [et al.] -- Generation and neuronal Differentiation of patient-specific induced pluripotent stem cells derived from Niemann-Pick type C1 fibroblasts / Michaela Trilck, Rayk Hubner, and Moritz J. Frech -- Multisystemic disease modeling of liver-derived protein folding disorders using induced pluripotent stem cells (iPSCs) / Amy Leung and George J. Murphy -- In vitro modeling of alcohol-induced liver injury using human-induced pluripotent stem cells / Lipeng Tian, Neha Prasad, and Yoon-Young Jang -- Generation of human induced pluripotent stem cells using RNA-based sendai virus system and pluripotency validation of the resulting cell population / Valeria Chichagova ... [et al.] -- Modeling axonal phenotypes with human pluripotent stem cells / Kyle R. Denton, Chong-Chong Xu, and Xue-Jun Li -- Mitochondrial disease-specific induced pluripotent stem cell models : generation and characterization / Xuan Zhang ... [et al.] -- Patient-specific induced pluripotent stem cell models : characterization of iPS cell-derived cardiomyocytes / Toru Egashira ... [et al.] -- Generation of integration-free patient specific iPS cells using episomal plasmids under feeder free conditions / Sara Caxaria ... [et al.].
  • Digital
    Alexander Birbrair, editor.
    Springer2018
    This volume explores novel concepts of pericyte biology. The present book is an attempt to describe the most recent developments in the area of pericyte biology which is one of the emergent hot topics in the field of molecular and cellular biology today. Here, we present a selected collection of detailed chapters on what we know so far about the pericytes. Together with its companion volumes Pericyte Biology in Different Organs and Pericyte Biology in Disease, Pericyte Biology - Novel Concepts presents a comprehensive update on the latest information and most novel functions attributed to pericytes. To those researchers newer to this area, it will be useful to have the background information on these cells' unique history. It will be invaluable for both advanced cell biology students as well as researchers in cell biology, stem cells and researchers or clinicians involved with specific diseases. .
  • Digital
    editor, Alexander Birbrair.
    Springer2019
    This volume explores pericytes' roles under distinct pathological conditions, ranging from tumors, ALS, Alzheimers disease, Multiple Sclerosis, stroke, diabetes, atherosclerosis, muscular dystrophies and more. Together with its companion volumes Pericyte Biology in Different Organs and Pericyte Biology – Novel Concepts, Pericyte Biology in Disease presents a comprehensive update on the latest information and most novel functions attributed to pericytes. To those researchers newer to this area, it will be useful to have the background information on these cells' unique history. It will be invaluable for both advanced cell biology students as well as researchers in cell biology, stem cell biology and clinicians involved with these specific diseases.
  • Digital
    edited by Kyle J. Cetrulo [and others].
    Wiley2013
    Amniotic fluid stem cells / Sean Vincent Murphy and Anthony Atala -- Cord blood transplants : perinatal stem cells in clinical practice / Richard L. Haspel and Karen K. Ballen -- Hematopoietic stem cell development in the placenta / Katrin E.R. Ericson, Akanksha Chhabra, and Hanna K.A. Mikkolu -- Perinatal mesenchymal stem cell banking for umbilical cord blood transplantation and regenerative medicine / Rouzbeh R. Taghizadeh -- Making organ and stem cell transplantation safer : the role of mesenchymal stem cells / Hans Klingemann -- Wharton's jelly mesenchymal stem cells and immune modulation : regenerative medicine meets tissue repair / Rita Anzalone [and others] -- Immunogenicity versus immunomodulation of perinatal stem cells / Bram Lutton and Raimon Duran-Struuck -- The translational potential of perinatal stem cells in clinical medicine : mesenchymal stem cells / Radbeh Torabi [and others] -- Newborn stem cells : identity, function, and clinical potential / Anthony Park [and others] -- Biomedical potential of human perinatal stem cells / Oleg V. Semenov and Christian Breymann -- Progenitor cell therapy for the treatment of traumatic brain injury / Alex Bryan Olsen [and others] -- The human amniotic membrane : a tissue with multifaceted properties and different potential clinical applications / Maddalena Caruso, Antonietta Silini, and Ornella Parolini -- Advances and possible applications of human amnion for the management of liver disease / Fabio Marongiu [and others] -- Amnion-derived cells for stroke restorative therapy / Naoki Tajiri, Loren E. Glover, and Cesar V. Borlongan -- Pregnancy-acquired fetal progenitors as natural cell therapy / Elke Seppanen, Nicholas M. Fisk, and Kiarash Khosrotehrani -- Perinatal stem cells : an industry perspective / Kyle J. Cetrulo -- Patent protection of stem cell innovations / John R. Wetherell -- Interview with Frances Verter, founder of Parent's Guide to Cord Blood Foundation / Frances Verter and Kyle J. Cetrulo -- Umbilical cord blood banking : an obstetrician's perspective / Jordan H. Perlow.
  • Digital
    Zhong Chao Han, Tsuneo A. Takahashi, Zhibo Han, Zongjin Li, editors.
    Springer2019
    This book provides a comprehensive introduction to various types of perinatal stem cells. Given their unique regenerative abilities, stem cells offer a promising avenue in the treatment of degenerative diseases or injury. Currently, the limitations of postnatal cell sources and expanding efficiency may limit autologous stem cell therapies. Although embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can be cultured indefinitely ex vivo, and can differentiate into three germ layers, ethical issues, the teratoma formation of ESCs and oncogenic risk of iPSCs are major obstacles to their clinical application. More recently, perinatal stem cells have been isolated from the umbilical cord, Wharton's Jelly, placenta, amniotic membrane and amniotic fluid, which are normally discarded as medical waste. This book, after describing perinatal stem cells in detail, introduces readers to the various types of perinatal stem cells, addressing their characterization, banking, quality control and stability. Importantly, it also reviews the clinical applications of perinatal stem cells to therapy of diseases. Accordingly, it offers a valuable resource for clinicians, researchers and graduate students alike.
  • Digital
    edited by Anthony Atala, Kyle J. Cetrulo, Rouzbeh R. Taghizadeh, Sean V. Murphy, Curtis L. Cetrulo, Jr.
    ScienceDirect2018
    Perinatal Stem Cells provides researchers and clinicians with a comprehensive description of the current clinical and pre-clinical applications of stem cells derived from perinatal sources, such as amniotic fluid, placenta and placental membranes, the umbilical cord and Wharton's jelly. It's compiled by leading experts in the field, offering readers detailed insights into sources of perinatal stem cells and their potential for disease treatment. Therapeutic applications of perinatal stem cells include the treatment of in utero and pregnancy related diseases, cardiac disease, liver disease, pulmonary disease, inflammatory diseases, for hematopoietic regeneration, and for neural protection after stroke or traumatic brain injury. In addition, the rapid advance in clinical translation and commercialization of perinatal stem cell therapies is highlighted in a section on Clinical and Industry Perspective which provides insight into the new opportunities and challenges involved in this novel and exciting industry.
  • Digital
    edited by Kursad Turksen.
    Springer2011
    Computational modeling of the skin barrier / Arne Naegel, Michael Heisig, and Gabriel Wittum -- In vitro human skin segmentation and drug concentration-skin depth profiles / Ana Melero [and others] -- Transcriptional regulation of epidermal barrier formation / Ambica Bhandari [and others] -- Epidermal permeability barrier measurement in mammalian skin / Arup Kumar Indra and Mark Leid -- Assessment of permeability barriers to macromolecules in the rodent endometrium at the onset of implantation / Brent M. Bany and G. Scot Hamilton -- Assessment of intestinal permeability in (premature) neonates by sugar absorption tests / Willemijn E. Corpeleijn [and others] -- Analysis of epithelial cell shedding and gaps in the intestinal epithelium / Carrie A. Duckworth and Alastair J. Watson -- Studying permeability in a commonly used epithelial cell line : T84 intestinal epithelial cells / Rino P. Donato [and others] -- Optimization of the caco-2 permeability assay to screen drug compounds for intestinal absorption and efflux / Barry Press -- Ouabain modulates cell contacts as well as functions that depend on cell adhesion / Isabel Larre, Ruben G. Contreras, and Marcelino Cereijido -- Monitoring of the dynamics of epithelial dome formation using a novel culture chamber for long-term continuous live-cell imaging / Judith Lechner [and others] -- Measuring permeability in human retinal epithelial cells (ARPE-19) : implications for the study of diabetic retinopathy / Marta Garcia-Ramirez [and others] -- Analysis of epithelial barrier integrity in polarized lung epithelial cells / Monika Strengert and Ulla G. Knaus -- Permeability of differentiated human urothelium in vitro / Peter Rubenwolf and Jennifer Southgate -- Phenotyping the claudin 11 deficiency in testis : from histology to immunohistochemistry / Severine Mazaud-Guittot, Alexander Gow, and Brigitte Le Magueresse-Battistoni -- In vitro system to study sertoli cell blood-testis barrier dynamics / Dolores D. Mruk and C. Yan Cheng -- Analysis of endothelial barrier function in vitro / Yuping Wang and J. Steven Alexander -- Role of endothelial cell-cell junctions in endothelial permeability / Armelle Le Guelte and Julie Gavard -- In vitro analyses of endothelial cell permeability / Elizabeth Monaghan-Benson and Erika S. Wittchen -- Mechano-transduction and barrier regulation in lung microvascular endothelial cells / Kristina Giantsos, Mark Cluff, and Randal Dull -- Role of Caveolin-1 in the regulation of pulmonary endothelial permeability / Yu Sun, Richard D. Minshall, and Guochang Hu -- Assessment of endothelial permeability and leukocyte transmigration in human endothelial cell monolayers / Andreas Ludwig, Anselm Sommer, and Stefan Uhlig -- Permeability of endothelial Barrier : cell culture and in vivo models / Alexander N. Garcia [and others] -- Size-selective and in vitro assessment of inner blood retina barrier permeability / Matthew Campbell and Peter Humphries -- Assessment of permeability in barrier type of endothelium in brain using tracers : evans blue, sodium fluorescein, and horseradish peroxidase / Mehmet Kaya and Bulent Ahishali -- In vitro and in vivo methods for assessing FcRn-mediated reverse transcytosis across the blood-brain barrier / Nadia Caram-Salas [and others] -- Evaluation of VEGF-induced vascular permeability in mice / Sara M. Weis -- In vivo measurement of glioma-induced vascular permeability / Jisook Lee, Andrew Baird, and Brian P. Eliceiri -- In vivo optical imaging of ischemic blood-brain barrier disruption / Abedelnasser Abulrob [and others].
  • Digital
    edited by Michael Schrader.
    Springer2017
  • Digital
    Luis A. del Río, editor.
    Springer2013
    Peroxisomes are a class of ubiquitous and dynamic single membrane-bounded cell organelles, devoid of DNA, with an essentially oxidative type of metabolism. In recent years it has become increasingly clear that peroxisomes are involved in a range of important cellular functions in almost all eukaryotic cells. In higher eukaryotes, including humans, peroxisomes catalyze ether phospholipids biosynthesis, fatty acid alpha-oxidation, glyoxylate detoxification, etc, and in humans peroxisomes are associated with several important genetic diseases.
  • Digital
    Henning Ulrich, editor.
    Springer2010
  • Digital
    edited by Roberto Botelho.
    Springer2017
    This volume details experimental approaches used to investigate phagocytosis and phagosome maturation. Chapters present methods and protocols on quantifying uptake and phagosome maturation using biophysical and biochemical approaches, proteomics, microscopy, and flow cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phagocytosis and Phagosomes: Methods and Protocols aims to be an important resource for both experts in the field and for those investigators delving into phagocytosis and phagosome maturation for the first time.
  • Digital
    Dmitri V. Krysko, Peter Vandenabeele, editors.
    Springer2009
  • Digital
    edited by Kazushige Touhara.
    Springer2013
    Female moth pheromones / Tetsu Ando -- Pheromones in the fruit fly / Jacqueline S.R. Chin and Joanne Y. Yew -- Analysis of volatile mouse pheromones by gas chromatography mass spectrometry / Milos V. Novotny and Helena A. Soini -- Murine nonvolatile pheromones : isolation of exocrine-gland secreting peptide 1 / Hiroko Kimoto and Kazushige Touhara -- Chemical analysis of aquatic pheromones in fish / Michael Stewart, Cindy F. Baker, and Peter W. Sorensen -- Analysis of ascarosides from Caenorhabditis elegans using mass spectrometry and NMR spectroscopy / Xinxing Zhang ... [et al.] -- Identification of chemosensory receptor genes from vertebrate genomes / Yoshihito Niimura -- Functional assays for insect olfactory receptors in Xenopus oocytes / Tatsuro Nakagawa and Kaushige Touhara -- A protocol for heterologous expression and functional assay for mouse pheromone receptors / Sandeepa Dey, Senmiao Zhan, and Hiroaki Matsunami -- Genetic manipulation to analyze pheromone responses : knockouts of multiple receptor genes / Tomohiro Ishii -- Electroantennogram and single sensillum recording in insect antennae / Shannon B. Olsson and Bill S. Hansson -- Calcium imaging of pheromone responses in the insect antennal lobe / Susy M. Kim and Jing W. Wang -- Live cell calcium imaging of dissociated vomeronasal neurons / Angeldeep Kaur, Sandeepa De, and Lisa Stowers -- Whole-mount imaging of responses in mouse vomeronasal neurons / Pei Sabrina Xu and Timothy E. Holy -- Calcium imaging of vomeronasal organ response using slice preparations from transgenic mice expressing G-CaMP2 / C. Ron Yu -- The electrovomeronasogram : field potential recordings in the mouse vomeronasal organ / Trese Leinders-Zufall and Frank Zufall -- Electrical recordings from the accessory olfactory bulb in VNO-AOB ex vivo preparations / Julian P. Meeks and Timothy E. Holy -- Pheromone -induced expression of immediate early genes in the mouse vomeronasal sensory system / Sachiko Haga-Yamanaka and Kazushige Touhara -- Insect pheromone behavior : fruit fly / Daisuke Yamamoto, Soh Kohatsu, and Masayuki Koganezawa -- Quantitative assessment of pheromone-induced dauer formation in Caenorhabditis elegans / Scott J. Neal, Kyuhyung Kim, and Piali Sengupta -- Acute behavioral responses to pheromones in C. elegans (adult behaviors : attraction, repulsion ) / Heeun Jang and Cornelia I. Bargmann -- Behavioral analysis of pheromones in fish / Peter W. Sorensen -- Analysis of male aggressive and sexual behavior in mice / Takefumi Kikusui -- Assessment of urinary pheromone discrimination, partner preference, and mating behaviors in female mice / Olivier Brock, Julie Bakker, and Michael J. Baum.
  • Digital
    Paramjit S. Tappia, Naranjan S. Dhalla, editors.
    Springer2014
    Phospholipases generate lipid signaling molecules through their hydrolytic action on phospholipids and are known to regulate function of a variety of cells under normal and diseased conditions. While several physiological, biochemical and molecular techniques have identified key players involved in different disease processes, phospholipases have also emerged as critical players in the pathogenesis of a number of different diseases including cancer and heart disease. In addition, phospholipases are also implicated in such conditions as brain disorder/injury, kidney and immune cell dysfunction. Phospholipases in Health and Disease is a compilation of review articles dedicated to the study of the field with respect to biochemical and molecular mechanisms of normal and abnormal cell function. The wide range of area covered here is of interest to basic research scientists, clinicians and graduate students, who are engaged in studying pathophysiological basis of a variety of diseases. Furthermore, this book highlights the potential of the different phospholipases as therapeutic targets as well as part of prevention strategies. Twenty three articles in this book are organized in four sections that are designed to emphasize the most characterized forms of the phospholipases in mammalian cells. The first section discusses general aspect of phospholipases. Section two covers the role and function of phospholipase A in different pathophysiological conditions. The third section is focussed on phospholipase C which is believed to play a central role in transmembrane signaling. The final section covers phospholipase D which is present in a variety of different cells. The book illustrates that the activation of phospholipases is of fundamental importance in signal transduction affecting cell function. Overall, this book discusses the diverse mechanisms of phospholipase mediated signal transduction in different pathophysiological conditions and raises the possibility of specific forms of phospholipases serving as novel targets for drug development.
  • Digital
    Alexei Verkhratsky and Vladimir Parpura.
    Atypon2015
    Astrocytes can be defined as the glia inhabiting the nervous system with the main function in the maintenance of nervous tissue homeostasis. Classified into several types according to their morphological appearance, many of astrocytes form a reticular structure known as astroglial syncytium, owing to their coupling via intercellular channels organized into gap junctions. Not only do astrocytes establish such homocellular contacts, but they also engage in intimate heterocellular interactions with neurons, most notably at synaptic sites. As synaptic structures house the very core of information transfer and processing in the nervous system, astroglial perisynaptic positioning assures that these glial cells can nourish neurons and establish bidirectional communication with them, functions outlined in the concepts of the astrocytic cradle and multi-partite synapse, respectively. Astrocytes possess a rich assortment of ligand receptors, ion and water channels, and ion and ligand transporters, which collectively contribute to astrocytic control of homeostasis and excitability. Astroglia control glutamate and adenosine homeostasis to exert modulatory actions affecting the real-time operation of synapses. Fluctuations of intracellular calcium can lead to the release of various chemical transmitters from astrocytes through a process termed gliotransmission. Sodium fluctuations are closely associated to those of calcium with both dynamic events interfacing signaling and metabolism. Astrocytes appear fully integrated into the brain cellular circuitry, being an indispensable part of neural networks.
  • Digital
    Antonio Malvasi, Domenico Baldini, editors.
    Springer2020
    This richly illustrated book focuses on one of the specific and crucial steps of the Medically Assisted Procreation techniques that is often overlooked: the oocyte pick-up. Enhanced by a large number of high-quality pictures, this atlas of oocyte retrieval comprehensively describes important aspects such as the setting of the ultrasonic equipment, the choice of needle, the layout of the devices in the room and the technique to be used, which, if not properly handled, can lead to erroneous behaviors. The original and detailed illustrations, mainly microscopic images and explanatory drawings, help readers to better understand how to manage all the phases of the ovarian pick-up and oocyte management. This practical atlas provides a valuable guide for all specialists who want to improve their skills and gain confidence with the MPA techniques.
  • Digital
    Von G. Samedi, Therese Bocklage, .
    Springer2016
    Breast Cytology -- Female Reproductive System Cytology -- Salivary Gland Cytology -- Gastrointestinal Tract -- Pancreaticobiliary Tract -- Liver Cytology -- Respiratory Tract Cytology -- Urinary Tract Cytology -- Endocrine Cytology -- Body Cavity Fluids -- Lymph Nodes Cytology -- Bone and Soft Tissue Cytology -- Cerebrospinal Fluid Cytology.
  • Digital
    edited by Kursad Turksen.
    Springer2012
    Live imaging of Drosophila embryos : quantifying protein numbers and dynamics at subcellular locations / Daryl J.V. David [and others] -- Analyzing frizzled signaling using fixed and live imaging of the asymmetric cell division of the Drosophila sensory organ precursor cell / Bertrand Jauffred and Yohanns Bellaiche -- Protein-protein interaction techniques : dissect PCP signaling in Xenopus / Yingqun Wang -- Cuticle refraction microscopy : a rapid and simple method for imaging Drosophila wing topography, an alternative readout of wing planar cell polarity / David Neff, Justin Hogan, and Simon Collier -- Analysis of cell shape and polarity during Zebrafish gastrulation / Douglas C. Weiser and David Kimelman -- Analyzing planar cell polarity during Zebrafish gastrulation / Jason R. Jessen -- Wnt/planar cell polarity signaling in the regulation of convergent extension movements during Xenopus gastrulation / Gun-Hwa Kim, Edmond Changkyun Park, and Jin-Kwan Han -- Using 32-cell stage Xenopus embryos to probe PCP signaling / Hyun-Shik Lee [and others] -- Gene loss-of-function and live imaging in chick embryos / Anne C. Rios, Christophe Marcelle, and Olivier Serralbo -- Activation and function of small GTPases Rho, Rac, and Cdc42 during gastrulation / Courtney Mezzacappa, Yuko Komiya, and Raymond Habas -- Convergent extension analysis in mouse whole embryo culture / Sophie E. Pryor [and others] -- Analysis of PCP defects in mammalian eye lens / Yuki Sugiyama and John W. McAvoy -- Examining planar cell polarity in the mammalian cochlea / Helen May-Simera and Matthew W. Kelley -- Role of prickle1 and prickle2 in neurite outgrowth in murine neuroblastoma cells / Lisa Fujimura and Masahiko Hatano -- Planar cell polarity pathway and parietal endoderm cell migration / Kristi LaMonica and Laura Grabel -- Analysis of Wnt/planar cell polarity pathway in cultured cells / Mitsuharu Endo, Michiru Nishita, and Yasuhiro Minami -- Regulation of focal adhesion dynamics by Wnt5a signaling / Shinji Matsumoto and Akira Kikuchi -- Embryonic mouse gut tube as a model for analysis of epithelial polarity / Makoto Matsuyama and Akihiko Shimono -- Assessing PCP in the cochlea of mammalian ciliopathy models / Daniel J. Jagger and Andrew Forge -- Morphometric analysis of centrosome position in tissues / Hester Happe, Emile de Heer, and Dorien J.M. Peters.
  • Digital
    edited by Victor M. Loyola-Vargas, Neftalí Ochoa-Alejo.
    Springer2012
    Introduction to plant cell culture : the future ahead / Victor M. Loyola-Vargas and Neftali Ochoa-Alejo -- History of plant tissue culture / Trevor Thorpe -- Callus, cuspension Culture, and Hairy Roots. Induction, maintenance and characterization / Rosa M. Galaz-Avalos [and others] -- Growth measurements : estimation of cell division and cell expansion / Gregorio Godoy-Hernandez and Felipe A. Vazquez-Flota -- Measurement of cell viability / Lizbeth A. Castro-Concha, Rosa Maria Escobedo, and Maria de Lourdes Miranda-Ham -- Pathogen and biological contamination management in plant tissue culture : phytopathogens, vitro pathogens, and vitro pests / Alan C. Cassells -- Cryopreservation of embryogenic cell suspensions by encapsulation-vitrification and encapsulation-dehydration / Zhenfang Yin [and others] -- Study of in vitro development in plants : general approaches and photography / Edward C. Yeung -- Use of statistics in plant biotechnology / Michael E. Compton -- Tissue culture methods for the clonal propagation and genetic improvement of spanish red cedar (Cedrela odorata) / Yuri Pena-Ramirez [and others] -- Micropropagation of banana / Yıldız Aka Kacar and Ben Faber -- Liquid in vitro culture for the propagation of Arundo donax / Miguel Angel Herrera-Alamillo and Manuel L. Robert -- Production of haploids and doubled haploids in maize / Vanessa Prigge and Albrecht E. Melchinger -- Maize somatic embryogenesis : recent features to improve plant regeneration / Veronica Garrocho-Villegas, Maria Teresa de Jesus-Olivera, and Estela Sanchez Quintanar -- Improved shoot regeneration from root explants using an abscisic acid-containing medium / Subramanian Paulraj and Edward C. Yeung -- Cryopreservation of shoot tips and meristems : an overview of contemporary methodologies / Erica E. Benson and Keith Harding -- Anther culture of chili pepper (Capsicum spp.) / Neftali Ochoa-Alejo -- Production of interspecific hybrids in ornamental plants / Juntaro Kato and Masahiro Mii -- Plant tissue culture of fast-growing trees for phytoremediation research / Jose Luis Couselo [and others] -- Removing heavy metals by in vitro cultures / Maria del Socorro Santos-Diaz and Maria del Carmen Barron-Cruz -- Establishment of a sanguinarine-producing cell suspension culture of Argemone mexicana L (papaveraceae) : induction of alkaloid accumulation / Felipe A. Vazquez-Flota [and others] -- Epigenetics, the role of DNA methylation in tree development / Marcos Viejo [and others] -- Potential roles of microRNAs in molecular breeding / Qing Liu and Yue-Qin Chen -- Determination of histone methylation in mono- and dicotyledonous plants / Geovanny I. Nic-Can and Clelia De la Pena -- Basic procedures for epigenetic analysis in plant cell and tissue culture / Jose L. Rodriguez [and others] -- Plant tissue culture and molecular markers / Maria Tamayo-Ordonez [and others] -- Biolistic- and Agrobacterium-mediated transformation protocols for wheat / Cecilia Tamas-Nyitrai, Huw D. Jones, and Laszlo Tamas -- Improved genetic transformation of cork oak (Quercus suber L.) / Ruben Alvarez-Fernandez and Ricardo-Javier Ordas -- Organelle transformation / Anjanabha Bhattacharya [and others] -- Appendix A Components of the culture media / Victor M. Loyola-Vargas -- Appendix B Plant biotechnology and tissue culture resources in the internet / Victor M. Loyola-Vargas.
  • Digital
    edited by Víctor M. Loyola-Vargas, Neftalí Ochoa-Alejo.
    Springer2018
    Introduction to plant tissue culture : advances and perspectives / Victor M. Loyola-Vargas and Neftalí Ochoa-Alejo -- Micropropagation in the twenty-first century / Jean Carlos Cardoso, Lee Tseng Sheng Gerald, and Jaime A. Teixeira da Silva -- Cellular and morpho-histological foundations of in vitro plant regeneration / Diego Ismael Rocha, Lorena Melo Vieira, Andréa Dias Koehler, and Wagner Campos Otoni -- Bacterial endophytes in plant tissue culture : mode of action, detection, and control / Mona Quambusch and Traud Winkelmann -- Digital photography as a tool of research and documentation in plant tissue culture / Victor Gaba, Yehudit Tam, Danny Shavit, and Benjamin Steinitz -- Selection of molecular markers for the estimation of somaclonal variation / Octavio Martínez -- Plant tissue culture : a battle horse in the genome editing using CRISPR/Cas9 / Víctor M. Loyola-Vargas and Randy N. Avilez-Montalvo -- Micropropagation of agave species / Benjamín Rodríguez-Garay and José Manuel Rodríguez-Domínguez -- Protocol for the micropropagation of coconut from plumule explants / Luis Sáenz, José Luis Chan, María Narvaez, and Carlos Oropeza -- Micropropagation of yucca species / Yessica López-Ramírez, Alejandra Palomeque-Carlín, Lucía Isabel Chávez Ortiz, Ma. de Lourdes de la Rosa-Carrillo, and Eugenio Pérez-Molphe-Balch -- Auxin immunolocalization in Coffea canephora tissues / Ruth E. Márquez-López, Ángela Ku-González, Hugo A. Méndez-Hernández, Rosa M. Galaz-Ávalos, and Víctor M. Loyola-Vargas -- Somatic embryogenesis in common bean Phaseolus vulgaris L. / José Luis Cabrera-Ponce, Itzel Anayetzi González-Gómez, Claudia G. León-Ramírez, José A. Sánchez-Arreguín, and Alba E. Jofre y Garfias -- Induction of somatic embryogenesis in Jatropha curcas / Rosa M. Galaz-Ávalos, Heydi G. Martínez-Sánchez, and Víctor M. Loyola-Vargas -- In vitro proliferation of female buds for induction of somatic embryogenesis from false horn Plantain (AAB, cv. Curraré) / Rosa Maria Escobedo-Gracia-Medrano, Carlos Iván Cruz-Cárdenas, Lucila Aurelia Sánchez-Cach, José Roberto Ku-Cauich, and Wilma Aracely González-Kantún -- Somatic embryogenesis in Theobroma cacao L. / Claudia Garcia, Jean-Philippe Marelli, Juan Carlos Motamayor, and Cristiano Villela -- Somatic embryogenesis of Quercus suber L. from immature zygotic wmbryos / Pilar S. Testillano, Aránzazu Gómez-Garay, Beatriz Pintos, and María C. Risueño -- Cryotherapy : a novel method for virus eradication in economically important plant species / Min-Rui Wang, Long Chen, Zhibo Zhang, Dag-Ragnar Blystad, and Qiao-Chun Wang -- Cryopreservation of pineapple shoot tips by the droplet vitrification technique / Fernanda Vidigal Duarte Souza, Everton Hilo de Souza, Ergun Kaya, Lívia de Jesus Vieira, and Ronilze Leite da Silva -- Cryopreservation of pollen grains of pineapple and other bromeliads / Fernanda Vidigal Duarte Souza, Everton Hilo de Souza, and Ronilze Leite da Silva -- Application of in Casa pollination and embryo rescue techniques for breeding of agave species / Benjamín Rodríguez-Garay, Sigifredo López-Díaz, José Manuel Rodríguez-Domínguez, Antonia Gutiérrez-Mora, and Ernesto Tapia-Campos -- Haploid and doubled haploid plant production in carrot using induced parthenogenesis and ovule excision in vitro / Agnieszka Kiełkowska, Adela Adamus, and Rafal Baranski -- Using flow cytometry analysis in plant tissue culture derived plants / Rosa María Escobedo-Gracia-Medrano, Martha Josefa Burgos-Tan, José Roberto Ku-Cauich, and Adriana Quiroz-Moreno -- Procedure for estimating the tolerance and accumulation of heavy metals using plant cell cultures / Antonio Bernabé-Antonio, Amalia Maldonado-Magaña, María Elena Estrada-Zúñiga, Leticia Buendía-González, and Francisco Cruz-Sosa -- Proteomics as a tool to study molecular changes during plant morphogenesis in vitro / André Luis Wendt dos Santos, Ricardo Souza Reis, Angelo Schuabb Heringer, Eny Iochevet Segal Floh, Claudete Santa-Catarina, and Vanildo Silveira -- Proteomic analysis of non-model plant tissues using phenol extraction, two-dimensional electrophoresis, and MALDI mass spectrometry / Petra Peharec Štefanić, Mario Cindrić, and Biljana Balen -- Chromatin immunoprecipitation (ChiP) protocol for the analysis of gene regulation by histone modifications in Agave angustifolia haw / Rosa Us-Camas and Clelia De-la-Peña -- Transcription factors : their role in the regulation of somatic embryogenesis in Theobroma cacao L. and other species / Claudia Garcia, Dahyana Britto, and Jean-Philippe Marelli -- MicroRNA expression and regulation during maize somatic embryogenesis / Brenda Anabel López-Ruiz, Vasti Thamara Juárez-González, Elva Carolina Chávez-Hernández, and Tzvetanka D. Dinkova -- Elaboration of transcriptome during the induction of somatic embryogenesis / Elsa Góngora-Castillo, Geovanny I. Nic-Can, Rosa M. Galaz-Ávalos, and Víctor M. Loyola-Vargas -- Induction of specialized metabolism in in vitro cultures of Capsicum chinense Jacq / Felipe A. Vázquez-Flota and María de Lourdes Miranda-Ham -- Analysis of terpenoid indole alkaloids, carotenoids, phytosterols, and NMR-based metabolomics for Catharanthus roseus cell suspension cultures / Mohd Zuwairi Saiman, Natali Rianika Mustafa, and Robert Verpoorte -- Transformed root culture : from genetic transformation to NMR-based metabolomics / Andrey S. Marchev, Zhenya P. Yordanova, and Milen I. Georgiev -- Genetic transformation of Pentalinon andrieuxii tissue cultures / Yeseña Burgos-May, Elidé Avilés-Berzunza, Luis Manuel Peña-Rodríguez, and Gregorio Godoy-Hernández -- Appendix A : Components of the culture media.
  • Digital
    edited by Zoë A. Popper.
    Springer2011
    Plant tissue cultures / Anna Karkonen [and others] -- Computerized molecular modeling of carbohydrates / Alfred D. French and Glenn P. Johnson -- Oligosaccharide mass profiling (OLIMP) of cell wall polysaccharides by MALDI-TOF/MS / Markus Gunl, Florian Kraemaer, and Markus Pauly -- High-voltage paper electrophoresis (HVPE) of cell-wall building blocks and their metabolic precursors / Stephen C. Fry -- Carbohydrate gel electrophoresis / Florence Goubet, Paul Dupree, and Katja Salomon Johansen -- Monoclonal antibodies, carbohydrate-binding modules, and the detection of polysaccharides in plant cell walls / Cecile Herve, Susan E. Marcus, and J. Paul Knox -- Screening and characterization of plant cell walls using carbohydrate microarrays / Iben Sorensen and William G.T. Willats -- Electron tomography and immunogold labelling as tools to analyse de novo assembly of plant cell walls / Marisa S. Otegui -- Analysing cellulose biosynthesis with confocal microscopy / Meera Nair and Seth DeBolt -- Visual mapping of cell wall biosynthesis / Yumiko Sakuragi, Morten H.H. Norholm, and Henrick V. Scheller -- Atomic force microscopy of plant cell walls / Andrew R. Kirby -- Using solid-state 13C NMR spectroscopy to study the molecular organization of primary plant cell walls / Tracey J. Bootten [and others] -- Formation of cellulose-based composites with hemicelluloses and pectins using gluconacetobacter fermentation / Deirdre Mikkelsen and Michael J. Gidley -- Structural proteins of the primary cell wall : extraction, purification, and analysis / Derek T.A. Lamport, Li Tan, and Marcia J. Kieliszewski -- New insights into the control of cell growth / Claudia Blaukopf, Matthaus Z. Krol, and Georg J. Seifert -- Extraction and detection of Arabinogalactan proteins / Zoe A. Popper -- Characterization of plant cell wall proteome using high-throughput screens / Sang-Jik Lee and Jocelyn K.C. Rose -- Knocking out the wall : protocols for gene targeting in Physcomitrella patens / Alison W. Roberts [and others] -- Measuring in vitro extensibility of growing plant cell walls / Daniel J. Cosgrove.
  • Digital
    edited by Chris Hawes and Verena Kriechbaumer.
    Springer2018
    This volume presents a range of different techniques that have been used to characterize the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field.
  • Digital
    edited by Hans-Peter Mock and Andrea Matros, Katja Witzel.
    Springer2018
    Free flow zonal electrophoresis for fractionation of plant membrane compartments prior to proteomic analysis / Bronwyn J. Barkla -- Isolation and purity assessment of membranes from Norway spruce / Enni Vaisanen, Junko Takahashi, Luis A. Jimenez Barboza, Xianbao Deng, Teemu H. Teeri, Kurt V. Fagerstedt, Sabine Luthje, and Anna Kssarkonen -- Nuclear proteome : isolation of intact nuclei, extraction of nuclear proteins, and 2-DE analysis / Aarti Pandey, Subhra Chakraborty, and Niranjan Chakraborty -- Identification of plant nuclear proteins based on a combination of flow sorting, SDS-PAGE, and LC-MS/MS analysis / Ivo Chamrad, Jana Urinovska, Beata Petrovska, Hana Jerabkova, Rene Lenobel, Jan Vrana, Jaroslav Dolezel, and Marek Sebela -- Isolation, purity assessment, and proteomic analysis of nuclei / Setsuko Komatsu -- Proteomic analysis of rice golgi membranes isolated by floating through discontinuous sucrose density gradient / Kazusato Oikawa, Takuya Inomata, Yoshitoshi Hirao, Tadashi Yamamoto, Marouane Baslam, Kentaro Kaneko, and Toshiaki Mitsui -- Analyzing the vacuolar membrane (tonoplast) proteome / Miwa Ohnishi, Katsuhisa Yoshida, and Tetsuro Mimura -- Preparation of membrane fractions (envelope, thylakoids, grana, and stroma lamellae) from arabidopsis chloroplasts for quantitative proteomic investigations and other studies / Lucas Moyet, Daniel Salvi, Martino Tomizioli, Daphne Seigneurin-Berny, and Norbert Rolland -- Isolation of intact thylakoid membranes from heterocysts of filamentous, nitrogen-fixing cyanobacteria / Ann Magnuson and Tanai Cardona -- Targeted quantification of isoforms of a thylakoid-bound protein : MRM method development / Roque Bru-Martınez, Ascensi on Martınez-Marquez, Jaime Morante-Carriel, Susana Selles-Marchart, Marıa Jose Martınez-Esteso, Jose Luis Pineda-Lucas, and Ignacio Luque -- Sample preparation for analysis of the plant mitochondrial membrane proteome / Christine Schikowsky, Beate Thal, Hans-Peter Braun, and Holger Eubel -- Plasma membrane proteomics of Arabidopsis suspension-cultured cells associated with growth phase using Nano-LC-MS/MS / Bin Li, Daisuke Takahashi, Yukio Kawamura, and Matsuo Uemura -- Mini-scale isolation and preparation of plasma membrane proteins from potato roots for LC/MS analysis / Anna M. Jozefowicz, Andrea Matros, Katja Witzel, and Hans-Peter Mock -- Assay of plasma membrane H+-ATPase in plant tissues under abiotic stresses / Małgorzata Janicka, Anna Wdowikowska, and Grazyna Kłobus -- Absolute quantitation of in vitro expressed plant membrane proteins by targeted proteomics (MRM) for the determination of kinetic parameters / Carsten Rautengarten, Berit Ebert, and Joshua L. Heazlewood -- MSE for label-free absolute protein quantification in complex proteomes / Stefan Helm and Sacha Baginsky -- Identification and characterization of plant membrane proteins using ARAMEMNON / Rainer Schwacke and Ulf-Ingo Flugge -- VANTED : a tool for integrative visualization and analysis of -omics data / Anja Hartmann and Anna Maria Jozefowicz.
  • Digital
    edited by Laura De Gara and Vittoria Locato.
    Springer2018
  • Digital
    Robert B. Lorsbach, Marwan Yared, editors.
    Springer2016
    1. Introduction -- 2. Clinical features, management, and therapy of plasma cell neoplasms: what pathologists need to know -- 3. Morphologic and immunohistochemical evaluation of plasma cell neoplasms -- 4. Cytogenetics of plasma cell neoplasms -- 5. Flow cytometric analysis in the diagnosis and prognostication of plasma cell neoplasms -- 6. Renal manifestations of plasma cell neoplasms -- Index.
  • Digital
    Tiziana A.L. Brevini, Fulvio Gandolfi.
    Springer2013
    This addition to the Springer Brief in Stem Cells series focuses on aspects related to the specific mechanisms that ensure and control pluripotency and cell commitment in domestic animal species. This topic is generating rapidly-increasing interest due to the great potential for domestic animal species to be used as intermediate biomedical models, between the mouse and the human. The Brief addresses why we need large animal models for regenerative medicine. It also describes early embryo development with a careful and specific analysis of the regulatory mechanisms driving cleavage, polarization and genome activation in domestic species. How pluripotency is compartmentalized in domestic species as well as the different aspects that make the derivation of stem cells in domestic species very difficult are also addressed.
  • Digital
    edited by Uma Lakshmipathy, Mohan C. Vemuri.
    Springer2013
    1. Sources and derivation of human embryonic stem cells / Michal Amit -- 2. New chemical approach to the efficient generation of mouse embryonic stem cells / Hossein Baharvand and Seyedeh-Nafiseh Hassani -- 3. Review of the methods for human iPSC derivation / Nasir Malik and Mahendra S. Rao -- 4. Generation of human-induced pluripotent stem cells by lentiviral transduction / Jennifer C. Moore -- 5. Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus / Pauline T. Lieu, Andrew Fontes, Mohan C. Vemuri and Chad C. MacArthur -- 6. Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8 [trade mark] medium conditions / Andrew Fontes, Chad C. MacArthur, Pauline T. Lieu and Mohan C. Vemuri -- 7. Feeder-free substrates for pluripotent stem cell culture / Alexandria Sams and Mark J. Powers -- 8. Methods for culturing human embryonic stem cells on feeders / Jasmeet Kaur and Mary Lynn Tilkins -- 9. Methods for culturing human embryonic stem cells in a xeno-free system / Jasmeet Kaur, Mary Lynn Tilkins, Richard Eckert and Shayne Boucher -- 10. Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage / Dennis Van Hoof and Muluye E. Liku -- 11. Directed differentiation of pluripotent stem cells to functional hepatocytes / Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie -- 12. Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes / Paul W. Burridge and Elias T. Zambidis -- 13. Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors / Kejin Hu and Igor Slukvin -- 14. Cellular characterization of human pluripotent stem cells / Rene H. Quintanilla Jr. -- 15. TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells / Sunali N. Patel, Yalei Wu, Yun Bao, Ricardo Mancheo, Janice Au-Young and Elena Grigorenko -- 16. Epigenome analysis of pluripotent stem cells / Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart -- 17. Single cell gene expression analysis of pluripotent stem cells / Ronald V. Abruzzese and Richard A. Fekete -- 18. Profiling stem cells using quantitative PCR protein assays / David Ruff and Pauline T. Lieu -- 19. Labeled stem cells as disease models and in drug discovery / Catharina Ellerström, Raimund Strehl, and Johan Hyllner -- 20. Cloning technologies / Andrew Fontes -- 21. Stable transfection using episomal vectors to create modified human embryonic stem cells / Ying Liu, Kate Judd, and Uma Lakshmipathy -- 22. Site-specific integration in human ESC using Jump-In [trade mark] TI [trade mark] technology / Chad C. MacArthur.
  • Digital
    Kapil Bharti, editor.
    Springer2019
    This book discusses applications of pluripotent stem cells to study eye disease in vitro and to create novel therapies for degenerative eye diseases. Chapters are contributed by experts in the field and cover such topics as the use of pluripotent stem cells in 2D and 3D engineering of ocular tissues for disease modelling and drug testing as well as approaches to replace degenerated RPE and photoreceptors in macular degeneration and retinitis pigmentosa. Pluripotent Stem Cells in Eye Disease Therapy presents a comprehensive discussion of basic science and clinical applications and is an indispensable resource for everyone from advanced graduate students to advanced professionals who want to learn about the potential of stem cell biology and its role in the field of retinal diseases.

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