Books by Subject

Botany

  • Digital
    edited by Andy Pereira.
    Springer Protocols2011
    Analysis of High-Throughput Sequencing Data / Shrinivasrao P. Mane, Thero Modise, Bruno W. Sobral -- Identification of Plant microRNAs Using Expressed Sequence Tag Analysis / Taylor P. Frazier, Baohong Zhang -- Microarray Data Analysis / Saroj K. Mohapatra, Arjun Krishnan -- Setting Up Reverse Transcription Quantitative-PCR Experiments / Madana M. Ambavaram, Andy Pereira -- Virus-Induced Gene Silencing in Nicotiana benthamiana and Other Plant Species / Andrew Hayward, Meenu Padmanabhan, S. P. Dinesh-Kumar -- Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses / Zarir Vaghchhipawala, Clemencia M. Rojas, Muthappa Senthil-Kumar, Kirankumar S. Mysore -- Full-Length cDNA Overexpressor Gene Hunting System (FOX Hunting System) / Mieko Higuchi, Youichi Kondou, Takanari Ichikawa, Minami Matsui -- Activation Tagging with En/Spm-I /dSpm Transposons in Arabidopsis / Nayelli Marsch-Martínez, Andy Pereira -- Activation Tagging and Insertional Mutagenesis in Barley / Michael A. Ayliffe, Anthony J. Pryor -- Methods for Rice Phenomics Studies / Chyr-Guan Chern, Ming-Jen Fan, Sheng-Chung Huang, Su-May Yu, Fu-Jin Wei, Cheng-Chieh Wu, Arunee Trisiriroj, Ming-Hsing Lai, Shu Chen, Yue-Ie C. Hsing -- Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice / Sung-Ryul Kim, Jong-Seong Jeon, Gynheung An -- Transposon Insertional Mutagenesis in Rice / Narayana M. Upadhyaya, Qian-Hao Zhu, Ramesh S. Bhat -- Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants / Xiaofei Cheng, Jiangqi Wen, Million Tadege, Pascal Ratet, Kirankumar S. Mysore -- Screening Arabidopsis Genotypes for Drought Stress Resistance / Amal Harb, Andy Pereira -- Protein Tagging for Chromatin Immunoprecipitation from Arabidopsis / Stefan Folter -- Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions / Pieter B. Ouwerkerk, Annemarie H. Meijer -- Plant Metabolomics by GC-MS and Differential Analysis / Joel L. Shuman, Diego F. Cortes, Jenny M. Armenta, Revonda M. Pokrzywa, Pedro Mendes, Vladimir Shulaev -- Gramene Database: A Hub for Comparative Plant Genomics / Pankaj Jaiswal.
  • Digital
    edited by Sergey Shabala, Tracey Ann Cuin.
    Springer Protocols2012
    Patch-clamp protocols to study cell ionic homeostasis under saline conditions / Ana Maria Velarde-Buendia, Rene Alberto Enriquez-Figueroa, and Igor Pottosin -- Studying plant salt tolerance with the voltage clamp technique / Zhong-Hua Chen [and others] -- Using the multifunctional xylem probe for in situ studies of plant water and ion relations under saline conditions / Lars H. Wegner -- Measuring intracellular ion concentrations with multi-barrelled microelectrodes / Anthony J. Miller and Susan Smith -- Single-cell sampling and analysis (SiCSA) / Wieland Fricke -- Non-invasive flux measurements using microsensors : theory, limitations, and systems / Ian Newman [and others] -- Quantifying kinetics of net ion fluxes from plant tissues by non-invasive microelectrode measuring MIFE technique / Sergey Shabala [and others] -- Quantitative cryo-analytical scanning electron microscopy (CEDX) : an important technique useful for cell-specific localization of salt / Margaret McCully and Martin Canny -- Fluorescence lifetime imaging (FLIM) measurements in salinity research / Olga Babourina and Zed Rengel -- Cytosolic Ca² determinations in studying plant responses to salinity and oxidative stress / Anuphon Laohavisit, Renato Colaco, and Julia Davies -- Infrared thermography in plant phenotyping for salinity tolerance / Richard A. James and Xavier R.R. Sirault -- In vivo imaging of nitric oxide and reactive oxygen species using laser scanning confocal microscopy / Yan-Jie Xie and Wen-Biao Shen -- Metabolomics for salinity research / Ute Roessner and Diane M. Beckles -- Purification of plant plasma membranes by two-phase partitioning and measurement of H⁺ pumping / Anette Lund and Anja Thoe Fuglsang -- Determination of reactive oxygen species in salt-stressed plant tissues / Andres Alberto Rodriguez and Edith L. Taleisnik -- Quantification of the antioxidant activity in salt-stressed tissues / Jelena J. Dragisic Maksimovic and Branka D. Zivanovic -- Quantification of abscisic acid, cytokinin, and auxin content in salt-stressed plant tissues / Petre I. Dobrev and Radomira Vankova -- Fluorescence-activated cell sorting for analysis of cell type-specific responses to salinity stress in Arabidopsis and rice / Aurelie Evrard [and others] -- Transformation using controlled cDNA overexpression system / Gabor Rigo, Csaba Papdi, and Laszlo Szabados -- Transcriptome analysis of membrane transporters in response to salinity stress / Prasad Senadheera and Frans J.M. Maathuis -- Marker-assisted selection in plant breeding for salinity tolerance / M. Ashraf [and others] -- transcriptomics on small samples / Stuart J. Roy [and others] -- Plastid transformation for abiotic stress tolerance in plants / K.C. Bansal, A.K. Singh, and S.H. Wani -- Manipulating expression of tonoplast transporters / Zhigang Li [and others] -- Using heterologous expression systems to characterize potassium and sodium transport activities / Alonso Rodriguez, Begona Benito, and Olivier Cagnac -- Isotope techniques to study kinetics of Na⁺ and K⁺ transport under salinity conditions / Dev T. Britto and Herbert J. Ronzucker -- Trait dissection of salinity tolerance with plant phenomics / Bettina Berger, Bas de Regt, and Mark Tester -- Measuring soil salinity / Marcus Hardie and Richard Doyle.
  • Digital
    edited by Thomas Pfannschmidt.
    Springer Protocols2009
    Multiple dimensions in plant signal transduction : an overview / Jorge J. Casal -- Phenotyping of Arabidopsis mutants for developmental effects of gene deletions / Cordelia Bolle -- Phenotyping of abiotic responses and hormone treatment in Arabidopsis / Cordelia Bolle -- Accurate real-time reverse transcription quantitative PCR / Marco Klatte and Petra Bauer -- Probing spatio-temporal intracellular calcium variations in plants / Axel Mithöfer, Christian Mazars, and Massimo E. Maffei -- Dynamic redox measurements with redox-sensitive GFP in plants by confocal laser scanning microscopy / Andreas J. Meyer and Thorsten Brach -- Imaging reactive oxygen species in vivo / Steven M. Driever ... [et al.] -- Identification of thioredoxin targeted proteins using thioredoxin single cysteine mutant-immobilized resin / Ken Motohashi, Patrick G.N. Romano, and Toru Hisabori -- Determination of in vivo protein phosphorylation in photosynthetic membranes / Julia P. Vainonen, Alexander V. Vener, and Eva-Mari Aro -- Examining protein stability and its relevance for plant growth and development / Claus Schwechheimer ... [et al.] -- Probing circadian rhythms in Chlamydomonas rheinhardtii by functional proteomics / Volker Wagner and Maria Mittag -- Bimolecular fluorescence complementation (BiFC) to study protein-protein interactions in living plant cells / Katia Schütze, Klaus Harter, and Christina Chaban -- Fluorescence cross-correlation spectroscopy of plant proteins / Hideki Muto, Masataka Kinjo, and Kotaro T. Yamamoto -- The determination of protein-protein interactions by the mating-based split-ubiquitin-system (mbSUS) / Christopher Grefen, Petr Obrdlik, and Klaus Harter -- Functional complementation of yeast mutants to study plant signalling pathways / Norbert Mehlmer, Elisabeth Scheikl-Pourkhalil, and Markus Teige -- Phosphatase activities analyzed by in vivo expressions / Alois Schweighofer, Zahra Ayatollahi, and Irute Meskiene -- Chromatin immunoprecipitation experiments to investigate in vivo binding of Arabidopsis transcription factors to target sequences / Benjamin Fode and Christiane Gatz -- Fluorescence-based electrophoretic mobility shift assay in the analysis of DNA-binding proteins / Sebastian Steiner and Thomas Pfannschmidt -- Analysis of plant regulatory DNA sequences by the yeast-one-hybrid assay / Dierk Wanke and Klaus Harter -- Analysis of plant regulatory DNA sequences by transient protoplast assays and computer aided sequence evaluation / Kenneth W. Berendzen, Klaus Harter, and Dierk Wanke -- RNA protein interaction mediating post-transcriptional regulation in the circadian system / Jan C. Schöning and Dorothee Staiger.
  • Digital
    edited by Jose R. Botella, Miguel A. Botella.
    Springer Protocols2016
    Suppressor screens in Arabidopsis / Xin Li and Yuelin Zhang -- Quantitative reverse transcription-qPCR-based gene expression analysis in plants / Heithem Ben Abdallah and Petra Bauer -- DNA-binding factor target identification by chromatin immunoprecipitation (ChIP) in plants / David Posé and Levi Yant -- Identification of genes responsible for natural variation in volatile content using next-generation sequencing technology / Iraida Amaya, Jeremy Pillet, and Kevin M. Folta -- Micro-tom tomato as an alternative plant model system : mutant collection and efficient transformation / Masahito Shikata and Hiroshi Ezura -- Culture of the tomato micro-tom cultivar in greenhouse / Christophe Rotha ... [et al.] -- Virus-induced gene silencing as a tool to study tomato fruit biochemistry / Elio Fantini and Giovanni Giuliano -- Simplified and rapid method for the isolation and transfection of Arabidopsis leaf mesophyll protoplasts for large-scale applications / Arnaldo L. Schapire and L. Maria Lois -- Preparation of epidermal peels and guard cell protoplasts for cellular, electrophysiological, and -omics assays of guard cell function / Mengmeng Zhu ... [et al.] -- Application of variable angle total internal reflection fluorescence microscopy to investigate protein dynamics in intact plant cells / Yinglang Wan ... [et al.] -- Immunoprecipitation of plasma membrane receptor-like kinases for identification of phosphorylation sites and associated proteins / asuhiro Kadota, Alberto P. Macho, and Cyril Zipfel -- Yeast three-hybrid system for the detection of protein-protein interactions / Natsumi Maruta, Yuri Trusov, and Jose R. Botella -- Cautions in measuring in vivo interactions using FRET and BiFC in Nicotiana benthamiana / Meral Tunc-Ozdemir, Yan Fu, and Alan M. Jones -- Analysis of protein-lipid interactions using purified C2 domains / Jessica Pérez-Sancho ... [et al.] -- Assessing kinase activity in plants with in-gel kinase assays / Pengcheng Wang and Jian-Kang Zhu -- Analyses of plant UDP-dependent glycosyltransferases to identify their volatile substrates using recombinant proteins / Yusuke Kamiyoshihara, Denise M. Tieman, and Harry J. Klee -- Competitive index : mixed infection-based virulence assays for genetic analysis in Pseudomonas syringae-plant interactions / Alberto P. Macho ... [e tal.] -- Simplified assays for evaluation of resistance to Alternaria brassicicola and turnip mosaic virus / Yuri Trusov ... [et al.] -- Simultaneous determination of plant hormones by GC-TOF-MS / José G. Vallarino and Sonia Osorio.
  • Digital
    edited by Zhi-Yong Wang, Zhenbiao Yang.
    Springer Protocols2012
    Experimental analysis of receptor kinase phosphorylation / Srijeet K. Mitra, Michael B. Goshe, and Steven D. Clouse -- Quantitative measurement of phosphopeptides and proteins via stable isotope labeling in Arabidopsis and functional phosphoproteomic strategies / Ning Li -- Identification of o-linked Beta-D-N-acetylglucosamine-modified proteins from Arabidopsis / Shou-Ling Xu [and others] -- Quantitative analysis of protein phosphorylation using two-dimensional difference gel electrophoresis / Zhiping Deng, Shuolei Bu, and Zhi-Yong Wang -- Quantitative analysis of plasma membrane proteome using two-dimensional difference gel electrophoresis / Wenqiang Tang -- Identification and verification of redox-sensitive proteins in Arabidopsis thaliana / Hai Wang, Shengbing Wang, and Yiji Xia -- Small-molecule dissection of brassinosteroid signaling / Mirela-Corina Codreanu [and others] -- Chemical genetics method to uncover small molecules for dissecting the mechanism of ABA responses in Arabidopsis seed germination / Yang Zhao -- Activation tagging / Xiaoping Gou and Jia Li -- Rho GTPase activity analysis in plant cells / Tongda Xu -- Analysis of in vivo ROP GTPase activity at the subcellular level by fluorescence resonance energy transfer microscopy / Lei Zhu and Ying Fu -- In vivo ubiquitination assay by agroinfiltration / Lijing Liu, Qingzhen Zhao, and Qi Xie -- In vitro protein ubiquitination assay / Qingzhen Zhao, Lijing Liu, and Qi Xie -- Genome-wide identification of transcription factor-binding sites in plants using chromatin immunoprecipitation followed by microarray (ChIP-chip) or sequencing (ChIP-seq) / Jia-Ying Zhu, Yu Sun, and Zhi-Yong Wang -- Smart pooling of mRNA samples for efficient transcript profiling / Raghunandan M. Kainkaryam [and others] -- Transient expression assays for quantifying signaling output / Yajie Niu and Jen Sheen -- Genome-wide profiling of uncapped mRNA / Yuling Jiao and Jose Luis Riechmann -- Computational tools for quantitative analysis of cell growth patterns and morphogenesis in actively developing plant stem cell niches / Anirban Chakraborty [and others]p.
  • Digital
    edited by Ling Yuan, Sharyn E. Perry.
    Springer Protocols2011
    1. MADS and more: transcription factors that shape the plant / Rainer Melzer and Günter Thei€en -- 2. In silico mining and PCR-based approaches to transcription factor discovery in non-model plants: gene diversity of the WRKY transcription factors in conifers / Jun-Jun Liu and Yu Xiang -- 3. Isolation of plant transcription factors using a yeast one-hybrid system / Tatiana Pyvovarenko and Sergiy Lopato -- 4. A transposon-based activation tagging system for gene function discovery in arabidopsis / Nayelli Marsch-Martinez -- 5. CRWA-T, an effective gene silencing system utilizing chimeric repressors / Nobutaka Mitsuda, Kyoko Matsui, Miho Ikeda, Masaru Nakata, Yoshimi Oshima, Yukari Nagatoshi, and Masaru Ohme-Takagi -- 6. Analysis of a transcription factor using transient assay in arabidopsis protoplasts / Yuji Iwata, Mi-Hyun Lee, and Nozomu Koizumi -- 7. Microarray-based identification of transcription factor target genes / Maartje Gorte, Anneke Horstman, Robert B. Page, Renze Heidstra, Arnold Stromberg and Kim Boutilier -- 8. Yeast protein-protein interaction assays and screens / Stefan de Folter and Richard G.H. Immink -- 9. Mapping functional domains of transcription factors / Ling Zhu and Enamul Huq -- 10. Biomolecular fluorescence complementation as a tool to study interactions of regulatory proteins in plant protoplasts / Sitakanta Pattanaik, Joshua R. Werkman, and Ling Yuan -- 11. Isolation of transcription factor complexes from arabidopsis cell suspension cultures by tandem affinity purification / Jelle Van Leene, Dominique Eeckhout, Geert Persiau, Eveline Van De Slijke, Jan Geerinck, Gert Van Isterdael, Erwin Witters, and Geert De Jaeger -- 12. Assaying transcription factor stability / Jasmina Kurepa and Jan A. Smalle -- 13. How to access the intercellular trafficking of transcription factors / Munawar Ahmad, Won Kyong Cho, Yeonggil Rim, Lijun Huang, and Jae-Yean Kim -- 14. SELEX (systematic evolution of ligands by exponential enrichment), as a powerful tool for deciphering the protein-DNA interaction space / Chenglin Chai, Zidian Xie, and Erich Grotewold -- 15. Footprinting and missing nucleoside analysis of transcription factor-DNA complexes / Ivana L. Viola and Daniel H. Gonzalez -- 16. Chromatin immunoprecipitation to verify or to identify in vivo protein-DNA interactions / Yumei Zheng and Sharyn E. Perry -- 17. Visualizing and characterizing invivo DNA-binding events and direct target genes of plant transcription factors / Jose M. Muiño, Gerco C. Angenent, and Kerstin Kaufmann -- 18. Mapping in vivo protein-DNA interactions in plants by DamID, a DNA adenine methylation-based method / Sophie Germann and Valérie Gaudin -- 19. Directed evolution through DNA shuffling for the improvement and understanding of genes and promoters / Joshua R. Werkman, Sitakanta Pattanaik, and Ling Yuan.
  • Digital
    Marie-Angèle Grandbastien, Josep M. Casacuberta, editors.
    Springer2012
    Transposable elements are DNA sequences with the capacity to move within a genome. Although their presence and impact has long been known, the recent genome-wide analysis of many eukaryotic genomes has uncovered their major role in genome dynamics and function. The present book explains how to recognize and study transposable elements, e.g. by using state-of-the-art strategies based on new-generation sequencing. Moreover, the impact of transposable elements on plant genome structure and function is reviewed in detail and illustrated in examples and case studies. The book is intended both for readers familiar with the field and for newcomers. With large-scale sequencing becoming increasingly available, more and more people will come across transposable element sequences in their data, and this volume will hopefully help to convince them that transposable elements are not just "junk" DNA, and may actually be the most interesting and fun part of their data!
  • Digital
    edited by Thomas Peterson.
    Springer Protocols2013
    Historical overview of transposable element research / P.A. Peterson -- Distinguishing variable phenotypes from variegation caused by transposon activities / V. Walbot -- Using transposons for genetic mosaic analysis of plant development / P.W. Becraft -- Survey of natural and transgenic gene markers used to monitor transposon activity / L. Krishnaswamy and T. Peterson -- Molecular biology of maize Ac/Ds elements: An overview / K. Lazarow, M.L. Doll and R. Kunze -- Gene tagging with engineered Ds elements in maize / Y. Li, G. Segal, Q. Wangm H.K. Donner -- Plant regeneration methods for rapid generation of a large scale Ds transposant population in rice / Y.H. Xuan [and nine others] -- Isolation of sequences flanking Ac insertion sites by Ac casting / D. Wang and T. Peterson -- Regulation of the mutator system of transposons in maize / D. Lisch -- Using MuDR/Mu transposons in directed tagging strategies / V. Walbot and J. Questa -- Genetic and molecular analyses of UniformMu transposon insertion lines / D.R. McCarty, M. Suzuki, C. Hunter, J. Collins, W.T. Avigne, K.E. Koch -- Digestion-ligation-amplification (DLA): A simple genome walking method to amplify unknown sequences flanking mutator (Mu) transposons and thereby facilitate gene cloning / S. Liu, A.P. Hsia and P.S. Schnable -- Molecular genetics and epigenetics of CACTA elements / N.V. Fedoroff -- Activation tagging using the maize En-I transposon system for the identification of abiotic stress resistance genes in arabidopsis / A. Harb and A. Pereira -- Reverse genetics in rice using Tos17 / D. Mieulet, A. Dievart, G. Droc, N. Lanau, E. Guiderdoni -- Identification and applications of the Petunia Class II Act1/dTph1 transposable element system / T. Gerats, J. Zethof and M. Vandenbussche -- Transposon display: A versatile method for transposon tagging / M. Vandenbussche, J. Zethof and T. Gerats -- Massive indexed parallel identification of transposon flanking sequences / M. Vandenbussche, J. Zethof and T. Gerats -- Use of next generation sequencing (NGS) technologies for the genome-wide detection of transposition / M. Elbaidouri, C. Chaparro and O. Panaud -- Overview of repeat annotation and de novo repeat identification / N. Jiang -- Computational methods for identification of DNA transposons / N. Jiang -- Tenest 2.0: Computational annotation and visualization of nested transposable elements / B.A. Kronmiller and R.P. Wise.
  • Digital
    Kenneth Palmer, Yuri Gleba, editors.
    Springer2014
    In this volume, the authors provide an excellent overview of how far the plant viral vector field has come. The discipline is no longer exclusively in the domain of academics-there is a small, but growing number of small biotechnology companies that exploit plant viruses as the platform for commercial innovation in crop improvement, industrial product manufacturing, and human and veterinary health care.
  • Digital
    editors: Gary D. Foster, I. Elisabeth Johansen, Yiguo Hong, Peter D. Nagy.
    Springer Protocols2008
  • Digital
    edited by Ichiro Uyeda and Chikara Masuta.
    Springer Protocols2015
    Detection of plant viruses in mixed infection by a macroarray-assisted method -- RT-PCR and real-time RT-PCR methods for the detection of potato virus Y in potato leaves and tubers -- A new method to isolate total dsRNA -- Multiplex RT-PCR method for the simultaneous detection of nine grapevine viruses -- Detection methods for rice viruses by a reverse-transcription loop-mediated isothermal amplification (RT-LAMP) -- Real-time PCR protocols for the quantification of the begomovirus tomato yellow leaf curl sardinia virus in tomato plants and in its insect vector -- Detection and analysis of non-retroviral RNA virus-like elements in plant, fungal, and insect genomes -- Detection of plant viruses in natural environments by using RNA-seq -- Cloning and profiling of small RNAs from cucumber mosaic virus satellite rna -- Drawing sirnas of viral origin out from plant siRNAs libraries -- Viral detection by high-throughput sequencing -- Analysis and application of viroid-specific small RNAs generated by viroid-inducing RNA silencing -- Detection and characterization of mycoviruses in arbuscular mycorrhizal fungi by deep-sequencing -- Supersage as an analytical tool for host and viral gene expression -- Microarray analysis of R-gene-mediated resistance to viruses -- Construction of infectious cDNA clones derived from the potyviruses clover yellow vein virus and bean yellow mosaic virus -- Virus-induced gene silencing of n gene in tobacco by apple latent spherical virus vectors -- Simplified methods for the construction of RNA and DNA virus infectious clones -- Efficient double-stranded RNA production methods for utilization in plant virus control -- Detection of plant virus in meristem by immunohistochemistry and in situ hybridization
  • Digital
    edited by Manfred Heinlein.
    Springer Protocols2015
    Plasmodesmata : channels for intercellular signaling during plant growth and development / Iris Sevilem, Shri Ram Yadav, and Ykä Helariutta -- Plasmodesmata : channels for viruses on the move / Manfred Heinlein -- Imaging plasmodesmata with high-resolution scanning electron microscopy / Deborah A. Barton and Robyn L. Overall -- Preparative methods for imaging plasmodesmata at super-resolution / Karen Bell and Karl Oparka -- Isolation of plasmodesmata from Arabidopsis suspension culture cells / Magali S. Grison, Lourdes Fernandez-Calvino, Sébastien Mongrand, and Emmanuelle M.F. Bayer -- Immunofluorescence detection of callose deposition around plasmodesmata sites / Ali Pendle and Yoselin Benitez-Alfonso -- Imaging callose at plasmodesmata using aniline blue : quantitative confocal microscopy / Raul Zavaliev and Bernard L. Epel -- Localization of fluorescently tagged protein to plasmodesmata by correlative light and electron microscopy / Shannon Modla, Jeffrey L. Caplan, Kirk J. Czymmek, and Jung-Youn Lee -- Quantification of plant cell coupling with live-cell microscopy / Johannes Liesche and Alexander Schulz -- Drop-ANd-See : a simple, real-time, and noninvasive technique for assaying plasmodesmal permeability / Weier Cui, Xu Wang, and Jung-Youn Lee -- Mapping symplasmic fields at the shoot apical meristem using iontophoresis and membrane potential measurements / Christiaan van der Schoot and Päivi L. H. Rinne -- Analysis of the conductivity of plasmodesmata by microinjection / Friedrich Kragler -- Investigating plasmodesmata genetics with virus-induced gene silencing and an agrobacterium-mediated GFP movement assay / Jacob O. Brunkard, Tessa M. Burch-Smith, Anne M. Runkel, and Patricia Zambryski -- Probing plasmodesmata function with biochemical inhibitors / Rosemary G. White -- GAL4 transactivation-based assay for the detection of selective intercellular protein Movement / Dhinesh Kumar, Huan Chen, Yeonggil Rim, and Jae-Yean Kim -- Techniques for assessing the effects of pharmacological inhibitors on intercellular protein movement / Shuang Wu and Kimberly L. Gallagher -- Probing protein targeting to plasmodesmata using fluorescence recovery after photo-bleaching / Kathryn M. Wright and Katrin M. MacKenzie -- Tracking of intercellular small RNA movement / Christophe Himber and Patrice Dunoyer -- Analysis of the role of myosins in targeting proteins to plasmodesmata / Martin Di Donato and Khalid Amari -- Pumilio-based RNA in vivo imaging / Jens Tilsner -- In vivo RNA labeling using MS2 / Eduardo Peña, Manfred Heinlein, and Adrian Sambade.
  • Digital
    by Michael Hesse, Heidemarie Halbritter, Reinhard Zetter, Martina Weber, Ralf Buchner ... [et al.].
    Springer2009
  • Digital
    edited by Arthur G. Hunt, Qingshun Quinn Li.
    Springer Protocols2015
    Computational analysis of plant polyadenylation signals / Xiaohui Wu , Guoli Ji , and Qingshun Quinn Li -- Prediction of plant mRNA polyadenylation sites / Xiaohui Wu , Guoli Ji , and Qingshun Quinn Li -- Extraction of poly(A) sites from large-scale RNA-seq data / Min Dong ... [et al.] -- Poly(A)-tag deep sequencing data processing to extract poly(A) sites / Xiaohui Wu, Guoli Ji, and Qingshun Quinn Li -- Analysis of poly(A) site choice using a java-based clustering algorithm / Patrick E. Thomas -- RADPRE : a computational program for identification of differential mRNA processing including alternative polyadenylation / Denghui Xing and Qingshun Quinn Li -- Characterization of plant polyadenylation complexes by using tandem affinity purification / Hongwei Zhao, Xinfu Ye, and Qingshun Quinn Li -- In vitro analysis of cleavage and polyadenylation in Arabidopsis / Hongwei Zhao and Qingshun Quinn Li -- Production, purification, and assay of recombinant proteins for in vitro biochemical analyses of the plant polyadenylation complex / Stephen A. Bell and Balasubrahmanyam Addepalli -- Detection of disulfide linkage by chemical derivatization and mass spectrometry / Balasubrahmanyam Addepalli -- Transient expression using agroinfiltration to study polyadenylation in plants / Carol Von Lanken and Arthur G. Hunt -- 3' RACE protocol to confirm polyadenylation sites / Liuyin Ma and Arthur G. Hunt -- Phage display library screening for identification of interacting protein partners / Balasubrahmanyam Addepalli, Suryadevara Rao, and Arthur G. Hunt -- Genome- wide determination of poly(A) site choice in plants / Pratap Kumar Pati, Liuyin Ma, and Arthur G. Hunt -- DNA/RNA hybrid primer mediated poly(A) tag library construction for illumina sequencing / Man Liu, Xiaohui Wu, and Qingshun Quinn Li -- Poly(A) tag library construction from 10 ng total RNA / Jingyi Cao and Qingshun Quinn Li -- Rapid, simple, and inexpensive method for the preparation of strand-specific RNA-seq libraries / Arthur G. Hunt -- Genome-wide analysis of distribution of RNA polymerase II isoforms using ChIP-Seq / Laura de Lorenzo.
  • Digital
    Kerry Bone, Simon Mills ; forewords Michael Dixon, Mark Blumenthal.
    ScienceDirect2013
    Machine generated contents note: pt. ONE Background and strategies -- 1.Herbal therapeutic systems -- 2.Principles of herbal pharmacology -- 3.Principles of herbal treatment -- 4.Validating herbal therapeutics -- 5.Optimising safety -- pt. TWO Practical clinical guides -- 6.Dosage and dosage forms in herbal medicine -- 7.A systematic approach to herbal prescribing -- 8.Herbal approaches to pathological states -- Topical applications -- Fever -- Infectious diseases -- Inflammatory and autoimmune diseases -- Fatigue and debility -- Malignant diseases -- 9.Herbal approaches to system dysfunctions -- Digestive system and bowel -- Biliary system -- The liver -- Cardiovascular system -- Respiratory system -- Urinary system -- Nervous system -- Female reproductive system -- Joint diseases -- Skin diseases -- Male reproductive system -- Endocrine disorders -- pt. THREE Materia Medica -- 10.How to use the monographs -- Andrographis -- Arnica flowers -- Astragalus -- Bearberry -- Contents note continued: Berberis bark and Hydrastis root -- Bilberry fruit -- Black cohosh -- Boswellia -- Buchu -- Bugleweed and European bugleweed -- Bupleurum -- Butcher's broom -- Chamomile, German -- Chaste tree -- Chelidonium -- Devil's claw -- Dong quai -- Echinacea root -- Evening primrose oil -- Eyebright -- Fennel fruit -- Feverfew -- Ginger -- Ginkgo -- Ginseng -- Globe artichoke -- Gotu kola -- Hawthorn -- Horsechestnut seed -- Kava -- Licorice -- Meadowsweet -- Melilotus -- Myrrh -- Nettle -- Pau d'arco -- Peppermint -- Poke root -- Rehmannia -- Saw palmetto -- Siberian ginseng -- St John's wort -- St Mary's thistle -- Thyme -- Tribulus leaf -- Turmeric -- Valerian -- Willow bark -- Witchhazel -- Withania.
  • Digital
    edited by S. Mohan Jain and Praveen K. Saxena.
    Springer Protocols2009
    Establishment of adventitious root cultures of Echinacea purpurea for the production of caffeic acid derivatives / K.Y. Paek, H.N. Murthy and E.J. Hahn -- In vitro propagation of Rauwolfia serpentina using liquid medium, assessment of genetic fidelity of micropropagated plants, and simultaneous quantitation of reserpine, ajmaline, and ajmalicine / M.K. Goel ... [et al.] -- Medicinal properties, in vitro protocols and secondary metabolite analyses of Scots pine / H. Haggman ... [et al.] -- Saussurea medusa cell suspension cultures for flavonoid production / C.Z. Liu and P.K. Saxena -- Large-scale in vitro multiplication of Crataeva nurvala / S.B. Babbar, N. Walia and A. Kaur -- Bilberry in vitro protocols and analyses of phenolic compounds / L. Jaakola ... [et al.] -- In vitro propagation of two tuberous medicinal plants: Holostemma ada-kodien and Ipomoea mauritiana / S.P. Geetha ... [et al.] -- In vitro production of gymnemic acid from Gymnema sylvestre (Retz) R. Br. Ex Roemer and Schultes through callus culture under abiotic stress conditions / A.B. Ali Ahmed, A.S. Rao and M.V. Rao -- Establishment of plant regeneration and cryopreservation system from zygotic embryo-derived embryogenic cell suspension cultures of Ranunculus kazusensis / S.W. Kim and M.J. Oh -- In vitro culture and secondary metabolite isolation in bryophytes / A. Sabovljevic, M. Sabovljevic and N. Jockovic -- Micropropagation and in vitro conservation of vanilla (Vanilla planifolia Andrews) / M. Divakaran and K.N. Babu -- Protocol for in vitro regeneration and marker glycoside assessment in Swertia chirata Buch-Ham / S. Koul ... [et al.] -- Protocols for establishment of an in vitro collection of medicinal plants in the genus Scutellaria / I.B. Cole, F.T. Farooq and S.J. Murch -- Protocols for in vitro culture and phytochemical analysis of Phyllanthus species (Euphorbiaceae) / E. Catapan ... [et al.] -- In vitro clonal propagation of Asparagus racemosus, a high value medicinal plant / S. Saxena and N. Bopana -- Micropropagation of Penthorum chinense through axillary buds / J. Yang and Z.S. Peng -- Spontaneous plant regeneration and production of secondary metabolites from hairy root cultures of Centaurium erythraea Rafn / A. Subotic ... [et al.] -- Transgenic Hypericum perforatum / G. Franklin, M.M. Oliveira and A.C. Dias -- Agrobacterium-mediated transformation of Ruta graveolens L / K. Lievre ... [et al.] -- Gene expression profiling in Taxus baccata L. seedlings and cell cultures / K. Brun akova and J. Kosuth -- Catapol production in Chinese foxglove (Rehmannia glutinosa Libos.) hairy roots transformed with Agrobacterium rhizogenes ATCC15834 / S.J. Hwang -- Identification of medicinal plants and plant sequences: multiplexed MLPA assay / R.A. Barthelson -- Isolation of microsatellites from Catharanthus roseus (L.) G. Don using enriched libraries / S. Bhatia and B. Shokeen -- Production of cinnamyl glycosides in compact callus aggregate cultures of Rhodiola rosea through biotransformation of cinnamyl alcohol / Z. Gyorgy and A. Hohtola -- Spearmint plantlet culture system as a means to study secondary metabolism / B. Tisserat, M. Berhow and S.F. Vaughn -- Bioreactor production of secondary metabolites from cell cultures of periwinkle and sandalwood / J.V. Valluri -- Camptothecin production by in vitro cultures and plant regeneration in Ophiorrhiza species / T. Asano ... [et al.] -- Metabolomic analysis of Ocotea odorifera cell cultures: a model protocol for acquiring metabolite data / M. Maraschin ... [et al.] -- Production of 9-methoxycanthin-6-one from callus cultures of (Eurycoma longifolia Jack) Tongkat Ali / M. Maziah and N. Rosli -- Plant secondary metabolism in altered gravity / L.K. Tuominen, L.H. Levine and M.E. Musgrave -- The role of biotechnology in the production of the anticancer compound podophyllotoxin / H. Lata, C.S. Mizuno and R.M. Moraes.
  • Digital
    edited Maurizio Lambardi, Elif Aylin Ozudogru, Shri Mohan Jain.
    Springer Protocols2013
    Micropropagation of pear (Pyrus sp.) / Barbara M. Reed [and others] -- In vitro propagation of jojoba / Berta E. Llorente and Nancy M. Apóstolo -- In vitro propagation of olive (Olea europaea L.) by nodal segmentation of elongated shoots / Maurizio Lambardi, Elif Aylin Ozudogru, and Romano Roncasaglia -- Micropropagation systems of feijoa (Acca sellowiana (O. Berg) burret) / Miguel Pedro Guerra [and others] -- Micropropagation of Vaccinium sp. by in vitro axillary shoot proliferation / Wojciech Litwinczuk -- In vitro propagation of peanut (Arachis hypogaea L.) by shoot tip culture / Elif Aylin Ozudogru, Ergun Kaya, and Maurizio Lambardi -- In vitro propagation of persimmon (Diospyros kaki Thunb.) / Edgardo Giordani, Mar Naval, and Carla Benelli -- Micropropagation of Citrus spp. by organogenesis and somatic embryogenesis / Benedetta Chiancone and Maria Antonietta Germanà -- Micropropagation of Prunus species relevant to cherry fruit production / Philippe Druart -- Micropropagation of peach rootstocks and cultivars / Ildikó Balla and Lucienne Mansvelt -- Micropropagation of Rubus and Ribes spp / Ewa Dziedzic and Joanna Jagła -- Somatic embryogenesis for efficient micropropagation of guava (Psidium guajava L.) / Nasim Akhtar -- Micropropagation of chokeberry by in vitro axillary shoot proliferation / Wojciech Litwińczuk -- Micropropagation of Lavandula spp / Sandra Gonçalves and Anabela Romano -- In vitro propagation of Acacia mangium and A. mangium? A. auriculiformis / Olivier Monteuuis, Antoine Galiana, and Doreen Goh -- In vitro propagation of ash (Fraxinus excelsior L.) by somatic embryogenesis / Maurizio Capuana -- Micropropagation of paradise tree (Melia azedarach) by in vitro culture of axillary buds / Luis A. Mroginski and Hebe Y. Rey -- In Vitro Propagation of Hydrangea spp / Barbara Ruffoni, Ermanno Sacco, and Marco Savona -- In vitro propagation of fraser photinia using Azospirillum-mediated root development / Berta E. Llorente and Ezequiel E. Larraburu -- Micropropagation of Helleborus through axillary budding / Margherita Beruto, Serena Viglione, and Alessandro Bisignano -- Micropropagation of Cordyline terminalis / Tui Ray, Prasenjit Saha, and Satyesh C. Roy -- Micropropagation of african violet (Saintpaulia ionantha Wendl.) / Mukund Shukla [and others] -- Micropropagation of Iris sp / Sladana Jevremovic, Zoran Jeknic, and Angelina Subotic -- Micropropagation of Gerbera (Gerbera jamesonii Bolus) / Ghani Minerva and Surinder Kumar -- Micropropagation of onion (Allium cepa L.) from immature inflorescences / Pablo Marinangeli -- In vitro propagation of cauliflower using curd microexplants / Martin Kieffer and Michael P. Fuller -- Micropropagation of Asparagus by in vitro shoot culture / Nataša Štajner -- Micropropagation and cryopreservation of garlic (Allium sativum L.) / E.R. Joachim Keller and Angelika Senula -- Micropropagation of globe artichoke (Cynara cardunculus L. var. scolymus) / Giovanni Iapichino -- In vitro rejuvenation of woody species / Paul E. Read and Christina M. Bavougian -- Encapsulation of in vitro- derived explants : an innovative tool for nurseries / Alvaro Standardi and Maurizio Micheli -- Thermotherapy, chemotherapy, and meristem culture in banana / Ludivine Lassois [and others] -- Agrobacterium-mediated genetic transformation of pineapple (Ananas comosus L., Merr.) / Minal Mhatre -- Protocol for inducing flower color somaclonal variation in Torenia (Torenia fournieri Lind.) / Duong Tan Nhut [and others] -- Production of pathogen-free horticultural crops by cryotherapy of in vitro-grown shoot tips / Chaohong Feng [and others].
  • Digital
    Da-Cheng Hao.
    ScienceDirect2018
    Ranunculales is an order of flowering plants belonging to a paraphyletic group known as the basal eudicots. Medicinal plants of this order provide myriad pharmaceutically active components commonly used worldwide in ethnomedicine and traditional Chinese medicine. Interest in plant-based medicinal resources has led to discoveries of many novel compounds in various species, and to investigations on their chemodiversity, biodiversity and pharmacotherapy. Ranunculales Medicinal Plants covers this order of flowering plants comprehensively in this context; detailing the phytochemistry, chemotaxonomy, molecular biology, and phylogeny of selected medicinal plants families and genera, and their relevance to drug efficacy. The book carries out an exhaustive survey of the literature in order to characterize global trends in the application of flexible technologies. The interrelationship between Chinese species and between Chinese and non-Chinese species is inferred, in the text, through molecular phylogeny, based on nuclear and chloroplast DNA sequencing. The title discusses the conflict between chemotaxonomy and molecular phylogeny, in the context of drug discovery and development. It offers invaluable and holistic coverage for the study of Ranunculales as a pharmaceutical resource.
  • Digital
    edited by Loïc Faye and Véronique Gomord.
    Springer Protocols2009
    From Neanderthal to nanobiotech : from plant potions to pharming with plant factories / Christophe Sourrouille ... [et al.] -- Cowpea mosaic virus-based systems for the expression of antigens and antibodies in plants / Frank Sainsbury, Li Liu, and George P. Lomonossoff -- Transient expression of antibodies in plants using syringe agroinfiltration / Marc-André D'Aoust ... [et al.] -- Rapid system for evaluating bioproduction capacity of complex pharmaceutical proteins in plants / Giuliana Medrano ... [et al.] -- Production and localization of recombinant pharmaceuticals in transgenic seeds / Thomas Rademacher, Elsa Arcalis, and Eva Stoger -- Production of antibody fragments in Arabidopsis seeds / Bart Van Droogenbroeck, Kirsten De Wilde, and Ann Depicker -- Production of plantibodies in Nicotiana plants / Marta Ayala ... [et al.] -- Physcomitrella patens : a non-vascular plant for recombinant protein production / David Liénard and Fabien Nogué -- Production of recombinant proteins in suspension-cultured plant cells / Carole Plasson ... [et al.] -- Chloroplast-derived vaccine antigens and biopharmaceuticals : protocols for expression, purification, or oral delivery and functional evaluation / N. Dolendro Singh, Yi Ding, and Henry Daniell -- Protein body induction : a new tool to produce and recover recombinant proteins in plants / Margarita Torrent, Imma Llop-Tous, and M. Dolors Ludevid -- A case study for plant-made pharmaceuticals comparing different plant expression and production systems / Guy Vancanneyt ... [et al.] -- Glycosylation of antibody therapeutics : optimisation for purpose / Roy Jefferis -- N-glycosylation of plant recombinant pharmaceuticals / Muriel Bardor ... [et al.] -- Companion protease inhibitors to protect recombinant proteins in transgenic plant extracts / Meriem Benchabane ... [et al.] -- Strategies for improving vaccine antigens expression in transgenic plants : fusion to carrier sequences / Jose M. Escribano and Daniel M. Perez-Filgueira -- Immunomodulation of plant function by in vitro selected single-chain Fv intrabodies / Manfred Gahrtz and Udo Conrad -- On-chip detection of low-molecular-weight recombinant proteins in plant crude extracts by SELDI-TOF MS / Amine M. Badri ... [et al.] -- Assessing the risk of undesirable immunogenicity/allergenicity of plant-derived therapeutic proteins / Paul D. Chamberlain -- Biosafety, risk assessment, and regulation of plant-made pharmaceuticals / Penelope A.C. Sparrow and Richard M. Twyman.
  • Digital
    edited by Yinong Yang.
    Springer Protocols2013
    Rice artificial hybridization for genetic analysis / Xueyan Sha -- Fluorescence in situ hybridization techniques for cytogenetic and genomic analyses / Jason G. Walling, Wenli Zhang, and Jiming Jiang -- Generation of rice mutants by chemical mutagenesis / Thomas H. Tai -- TILLING and ecotilling for rice / Jennifer L. Cooper [and others] -- Functional characterization of rice genes using a gene-indexed T-DNA insertional mutant population / Ki-Hong Jung and Gynheung An -- QTL analysis and map-based cloning of salt tolerance gene in rice / Ji-Ping Gao and Hong-Xuan Lin -- Site-specific gene integration in rice / Vibha Srivastava -- Transcriptome profiling analysis using rice oligonucleotide microarrays / Ki-Hong Jung and Pamela C. Ronald -- Cloning of small RNAs for the discovery of novel microRNAs in plants / Guru Jagadeeswaran and Ramanjulu Sunkar -- Global identification of small RNA targets in plants by sequencing sliced ends of messenger RNAs / Yong-Fang Li and Ramanjulu Sunkar -- Artificial microRNAs for specific gene silencing in rice / Norman Warthmann [and others] -- Rice proteomic analysis : sample preparation for protein identification / Ganesh Kumar Agrawal [and others] -- Quantification of jasmonic and salicylic acids in rice seedling leaves / Kyoungwon Cho [and others] -- Analysis of insect-induced volatiles from rice / Nan Zhao [and others] -- Phenotypic and physiological evaluation for drought and salinity stress responses in rice / Utlwang Batlang [and others] -- Phenotypic, physiological, and molecular evaluation of rice chilling stress response at the vegetative stage / Benildo G. de los Reyes [and others] -- Analysis of rice root hair morphology using cryo-scanning electron microscopy / Zhiming Yu -- Inoculation and virulence assay for bacterial blight and bacterial leaf streak of rice / Bing Yang and Adam Bogdanove -- Inoculation and scoring methods for rice sheath blight disease / Yulin Jia [and others] -- Molecular approaches to improve rice abiotic stress tolerance / Junya Mizoi and Kazuko Yamaguchi-Shinozaki -- Molecular strategies to improve rice disease resistance / Emily E. Helliwell and Yinong Yang -- Molecular strategies to engineer transgenic rice seed compartments for large-scale production of plant-made pharmaceuticals / Trevor Greenham and Illimar Altosaar.
  • Digital
    edited by Hiroaki Kodama, Atsushi Komamine.
    Springer Protocols2011
    Overview of plant RNAi / Yuichiro Watanabe -- Caveat of RNAi in plants : the off-target effect / Muthappa Senthil-Kumar and Kirankumar S. Mysore -- Plant gateway vectors for RNAi as a tool for functional genomic studies / Toshiya Muranaka -- Heat-inducible RNAi for gene functional analysis in plants / Frederic Masclaux and Jean-Philippe Galaud -- Gene function analysis by artificial microRNAs in Physcomitrella patens / Basel Khraiwesh [and others] -- Virus-induced gene silencing in ornamental plants / Cai-Zhong Jiang, Jen-Chih Chen, and Michael Reid -- Local RNA silencing mediated by agroinfiltration / Jutta Maria Helm, Elena Dadami, and Kriton Kalantidis -- Direct transfer of synthetic double-stranded RNA into protoplasts of Arabidopsis thaliana / Ha-il Jung, Zhiyang Zhai, and Olena K. Vatamaniuk -- Detection of long and short double-stranded RNAs / Toshiyuki Fukuhara [and others] -- Quantitative stem-loop RT-PCR for detection of microRNAs / Erika Varkonyi-Gasic and Roger P. Hellens -- Large-scale sequencing of plant small RNAs / William P. Donovan, Yuanji Zhang, and Miya D. Howell -- Computational prediction of plant miRNA targets / Ying-Hsuan Sun [and others] -- Bisulfite sequencing for cytosine-methylation analysis in plants / Nazmul Haque and Masamichi Nishiguchi -- Using nuclear run-on transcription assays in RNAi studies / Basel Khraiwesh -- Proteomic analysis of RNA interference induced knockdown plant / Sang Yeol Lee and Kyun Oh Lee -- Comparative analysis of phosphoprotein expression using 2D-DIGE / Tomoya Asano and Takumi Nishiuchi.
  • Digital
    Antonio Costa de Oliveira, Rajeev K. Varshney, editors.
    Springer2011
    With the predicted increase of the human population and the subsequent need for larger food supplies, root health in crop plants could play a major role in providing sustainable highly productive crops that can cope with global climate changes. While the essentiality of roots and their relation to plant performance is broadly recognized, less is known about their role in plant growth and development. "Root Genomics" examines how various new genomic technologies are rapidly being applied to the study of roots, including high-throughput sequencing and genotyping, Tilling, transcription.
  • Digital
    edited by Allison R. Kermode.
    Springer Protocols2011
    "Understanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying these processes will become increasingly important to many economical sectors and for species that provide the world's food supply. Seed Dormancy: Methods and Protocols covers analytical methods and approaches which have already lead to significant advances in the understanding of seed dormancy and germination. Chapters cover explanations of processes leading to the induction, maintenance, and termination of seed dormancy, the classification of different dormancy types, as well as an overview of protocols used for dormancy-termination of seeds of conifer species. This volume emphasizes methods essential for abscisic acid (ABA) analyses, including methods that have been important for receptor identification, analyses of ABA-catabolizing enzymes (the 8'-hydroxylases), and identification of novel signal transduction components, interacting partners, and/or response factors. The volume closes by addressing the development of new technologies, including spectroscopic methods (some of which allow for non-destructive sampling) as well as highly effective tissue-printing methods for seed dormancy research. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls."--Publisher's description.
  • Digital
    Eckart Eich.
    Springer2008
  • Digital
    Cathryn H. Greenberg, Beverly S. Collins, Frank R. Thompson III, editors.
    Springer2011
    This edited volume addresses a rising concern among natural resource scientists and management professionals about decline of the many plant and animal species associated with early-successional habitats, especially within the Central Hardwood Region of the USA. These open habitats, with herbaceous, shrub, or young forest cover, are disappearing as abandoned farmland, pastures, and cleared forest patches return to forest. There are many questions about 'why, what, where, and how' to manage for early successional habitats. In this book, expert scientists and experienced land managers synthesize knowledge and original scientific work to address critical questions sparked by the decline of early successional habitats.
  • Digital
    edited by Sarah E. O'Connor.
    ScienceDirect2016
    Directing biosynthesis: Practical supply of natural and unnatural cyanobactins / D. Sardar, M.D. Tianero, and E.W. Schmidt-- Synthetic biology approaches to new bisindoles/ L.M. Alkhalaf, Y.-L. Du, and K.S. Ryan-- Enzymatic [4+2] cycloadditions in the biosynthesis of Spirotetramates and spirotetronates/ B. Pang, G. Zhong, Z. Tang, and W. Liu-- Application and modification of flavin-dependent halogenases/ K.-H. van Pée, D. Milbredt, E.P. Patallo, V.Weichold, and M. Gajewi-- Engineering flavin-dependent halogenases/ J.T.Payne, M.C. Andorfer, and J.C. Lewis-- Heterologous expression of fungal secondary metabolite pathways in the Aspergillus nidulans host system/ J.W.A. van Dijk and C.C.C. Wang-- Plug-and-play benzylisoquinoline alkaloid biosynthetic gene discovery in engineered yeast-- J.S. Morris, M. Dastmalcji, J. Li, L. Chang, X. Chen, J.M. Hagel, and P.J. Facchini-- Optimizing metabolic pathways for the improved production of natural products/ J.A. Jones and M.A.G. Koffas-- Reconstituting plant secondary metabolism in Saccharomyces cerevisae for production of high-value benzylisoquinoline alkaloids/ M.E. Pyne, L. Narcross, E. Fossati, L. Bourgeois, E. Burton, N.D. Gold, and V.J.J. Martin-- Engineering microbes to synthesize plant isoprenoids/ K. Zhou, S. Edgar, and G. Stephanopoulos-- Natural product biosysnthesis in Escherichia coli: Menta monoterpenoids/ H.S. Toogood, S. Tait, A. Jervis, A. Ni Cheallaigh, L. Humphreys, E. Takano, J.M. Gardiner, and N.S. Scrutton-- High-efficiency genome editing of Streptomyces species by an engineered CRISPR/Cas system/ Y. Wang, R.E. Cobb, and H. Zhao-- Rapid optimization of engineered metabolic pathways with Serine Integrase Recombinational Assembly (SIRA)/ C.A. Merrick, C. Wardrope, J.E. Paget, S.D. Colloms, and S.J. Rosser-- Rewiring riboswitches to create new genetic circuits in bacteria/ C.J. Robinson, D. Medina-Stacey, M.-C. Wu, H.A. Vincent, and J. Micklefield.
  • Digital
    edited by Sarah E. O'Connor.
    ScienceDirect2016
  • Digital
    James F. Hancock, editor.
    Springer2008
  • Digital
    CRCnetBasev. 15-, 2015-
  • Digital
    edited by Baohong Zhang.
    Springer Protocols2013
    Transgenic cotton : from biotransformation methods to agricultural application / Baohong Zhang -- Genetically modified cotton in India and detection strategies / Gurinder Jit Randhawa and Rashmi Chhabra -- Agrobacterium-mediated transformation of cotton / Baohong Zhang -- Biolistic transformation of cotton zygotic embryo meristem / Kanniah Rajasekaran -- Biolistic transformation of cotton embryogenic cell suspension cultures / Kanniah Rajasekaran -- Cotton transformation via pollen tube pathway / Min Wang, Baohong Zhang, and Qinglian Wang -- Silicon carbide whisker-mediated transformation of cotton (Gossypium hirsutum L.) / Muhammad Arshad, Yusuf Zafar, and Shaheen Asad -- Investigating transgene integration and organization in cotton (Gossypium hirsutum L.) genome / Jun Zhang and Yan Hong -- Estimating the copy number of transgenes in transformed cotton by real-time quantitative PCR / Chengxin Yi and Yan Hong -- Development of enzyme-linked immunosorbent assay for the detection of Bt protein in transgenic cotton / Suchitra Kamle, Abhishek Ojha, and Arvind Kumar -- DNA-based diagnostics for genetically modified cotton : decaplex PCR assay to Differentiate MON531 and MON15985 Bt cotton events / Gurinder Jit Randhawa, Monika Singh, and Rashmi Chhabra -- Simple and rapid method for determining transgenic cotton plants / Baohong Zhang [and others] -- Efficient grafting technique for recovery of transgenic cotton plants / Min Wang, Qinglian Wang, and Baohong Zhang -- Inheritance of transgenes in transgenic Bt lines resistance to Helicoerpa armigera in upland cotton / Baolong Zhang, Wangzhen Guo, and Tianzhen Zhang -- Agrobacterium rhizogenes-induced cotton hairy root culture as an alternative tool for cotton functional genomics / Hee Jin Kim -- Overexpression of miR 156 in cotton via Agrobacterium-mediated transformation / Baohong Zhang [and others] -- Development of transgenic CryIA(c) + GNA cotton plants via pollen tube pathway method confers resistance to Helicoverpa armigera and Aphis gossypii Glover / Zhi Liu, Zhen Zhu, and Tianzhen Zhang -- Agrobacterium-mediated transformation of cotton (Gossypium hirsutum) shoot apex with a fungal phytase gene improves phosphorus acquisition / Zhiying Ma, Jianfeng Liu, and Xingfen Wang -- Genetic transformation of cotton with a harpin-encoding gene hpaXoo confers an enhanced defense response against Verticillium dahliae kleb / Weiguo Miao and Jingsheng Wang -- Development of insect-resistant transgenic cotton with chimeric TVip3A* accumulating in chloroplasts / Jiahe Wu and Yingchuan Tian -- Determining gene flow in transgenic cotton / Xiaoping Pan.
  • Digital
    edited by Jim M. Dunwell, Andy C. Wetten.
    Springer Protocols2012
    Employment of cytokinin vectors for marker-free and backbone-free transformation -- Organophosphorus hydrolase: A multifaceted plant genetic marker which is selectable, scorable, and quantifiable in whole seed -- Use of northern blotting for specific detection of small RNA molecules in transgenic plants -- Genetic transformation of the model green alga Chlamydomonas reinhardtii -- A high-efficiency agrobacterium-mediated transformation system of rice (Oryza sativa L.) -- Selection of transgenic rice plants using a herbicide tolerant form of the acetolactate synthase gene -- Visual selection in rice: A strategy for the efficient identification of transgenic calli accumulating transgene products -- Characterization of rice genes using a heterologous full-length cCDNA expression system -- Bioactive bead-mediated transformation of plants with large DNA fragments -- Agrobacterium-mediated transformation of sorghum bicolor using immature embryos -- Split-transgene expression in wheat -- Agrobacterium-mediated transformation of brachypodium distachyon -- Transformation of barley (Hordeum vulgare L.) by agrobacterium tumefaciens infection of in vitro cultured ovules -- Biolistic-mediated production of transgenic oil palm -- Transformation of oil palm using agrobacterium tumefaciens -- Highly efficient transformation protocol for plum (Prunus domestica L.) -- Co-transformation of grapevine somatic embryos to produce transgenic plants free of marker genes -- Initiation and transformation of grapevine embryogenic cultures -- Development of highly efficient genetic transformation protocols for table grape sugraone and crimson seedless -- Cotton pistil drip transformation method -- Enhanced agrobacterium-mediated transformation of embryogenic calli of upland cotton -- Targeted biolistics for improved transformation of impatiens balsamina -- A protocol for transformation of torenia -- Efficient modification of floral traits by heavy-ion beam irradiation on transgenic torenia -- Expression of artificial micrornas in physcomitrella patens -- High frequency of single-copy t-DNA transformants produced after floral dip in CRE-expressing arabidopsis plants -- A developmentally regulated cre-lox system to generate marker-free transgenic Brassica napus plants -- Exploiting multisite gateway and pENFRUIT plasmid collection for fruit genetic engineering -- A one-time inducible transposon to create knockout mutants in rice -- Marker-free gene targeting by recombinase-mediated cassette exchange -- Targeting DNA to a previously integrated transgenic locus using zinc finger nucleases -- Double-strand break-induced targeted mutagenesis in plants -- Combinatorial genetic transformation of cereals and the creation of metabolic libraries for the carotenoid pathway -- Production of a his-tagged canecystatin in transgenic sugarcane -- Plastid transformation as an expression tool for plant-derived biopharmaceuticals -- Use of a callus-specific selection system to develop transgenic rice seed accumulating a high level of recombinant protein -- How to grow transgenic arabidopsis in the field.
  • Digital
    edited by Huw D. Jones and Peter R. Shewry.
    Springer Protocols2009
    Transgenic wheat, barley and oats : production and characterization / Paul A. Lazzeri and Huw D. Jones -- Selection of transformed plants / Huw D. Jones and Caroline A. Sparks -- Reporter genes / Alison Huttly -- Biolistics transformation of wheat / Caroline A. Sparks and Huw D. Jones -- Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos / Huixia Wu, Angela Doherty and Huw D. Jones -- Floral transformation of wheat / Sujata Agarwal ... [et al.] -- Highly efficient agrobacterium-mediated transformation of wheat via in planta inoculation / Thierry Risacher ... [et al.] -- Barley transformation using biolistic techniques / Wendy A. Harwood and Mark A. Smedley -- Barley transformation using agrobacterium-mediated techniques / Wendy A. Harwood ... [et al.] -- Transformation of oats and its application to improving osmotic stress tolerance / Shahina B. Maqbool ... [et al.] -- Promoter sequences for defining transgene expression / Huw D. Jones and Caroline A. Sparks -- Down-regulation of gene expression by RNA-induced gene silencing / Silvia Travella and Beat Keller -- Gene insertion patterns and sites / Philippe Vain and Vera Thole -- Fluorescent in situ hybridization to detect transgene integration into plant genomes / Trude Schwarzacher -- Establishing substantial equivalence : transcriptomics / María Marcela Baudo ... [et al.] -- Establishing substantial equivalence : proteomics / Alison Lovegrove, Louise Salt, and Peter R. Shewry -- Establishing substantial equivalence : metabolomics / Michael H. Beale, Jane L. Ward, and John M. Baker -- Design and management of field trials of transgenic cereals / Zoltán Bedő, Mariann Rakszegi, and László Láng -- GM risk assessment / Penny A.C. Sparrow -- Transgenic wheat, barley and oats : future prospects / Jim M. Dunwell.
  • Digital
    edited by Annette Becker.
    Springer Protocols2013
    Virus-induced gene silencing (VIGS) in plants : an overview of target species and the virus-derived vector systems -- Rationale for developing new virus vectors to analyze gene function in grasses through virus-induced gene silencing -- Virus-induced gene silencing for rice using agroinoculation -- Utilizing virus-induced gene silencing for the functional characterization of maize genes during infection with the fungal pathogen ustilago maydis -- Analysis of developmental control genes using virus-induced gene silencing -- Virus-induced gene silencing in the rapid cycling columbine aquilegia coerulea Origami -- Virus-induced gene silencing of the alkaloid-producing basal eudicot model plant eschscholzia californica (California poppy) -- Virus-induced gene silencing using artificial miRNAs in nicotiana benthamiana -- The use of vigs technology to study plant-herbivore interactions -- Virus-aided gene expression and silencing using TRV for functional analysis of floral scent-related genes -- Virus-induced gene silencing in soybean and common bean -- Functional genomic analysis of cotton genes with agrobacterium-mediated virus-induced gene silencing -- Highly efficient virus-induced gene silencing in apple and soybean by apple latent spherical virus vector and biolistic inoculation -- VIGS : a tool to study fruit development in Solanum lycopersicum -- A protocol for VIGS in arabidopsis thaliana using a one-step TYMV-derived vector -- Virus-induced gene silencing in strawberry fruit.
  • Digital
    edited by Cletus P. Kurtzman, Jack W. Fell and Teun Boekhout.
    ScienceDirect2010
    Fully revised, updated and offered in a new two-volume format, The Yeasts: A Taxonomic Study, 5th Edition remains the most comprehensive presentation of yeast taxonomy and systematics available. Nearly 1500 species of ascomycete and basidiomycete yeasts are included, each description offering not only standard morphological and physiological characters, but also information on systematics, habitat, ecology, agricultural and biotechnological applications and clinical importance. Extensive introductory chapters discuss clinical aspects of yeasts, their role in biotechnology, food and beverage spoilage, agriculture and ecology, while other chapters include methodology for isolation of species from various habitats, phenotypic characterization, chemotaxonomy, gene sequence analysis and phylogenetics, including whole genome analysis. Additionally, easy-to-understand trees illustrate the phylogenetic placement of each species in its assigned genus as they have been determined from gene sequence analysis. This essential work, prepared by the leading experts in the field, is the most definitive treatment of taxonomy and systematics of yeasts on the market, and a necessary reference for any bookshelf or workbench. High quality photomicrographs and line drawings enhance world-class content Detailed trees clearly illustrate phylogenetic placement of each species in its assigned genus Discussion of clinical aspects of yeasts, including their role in biotechnology, agriculture and ecology help contextualize content for a wide range of researchers.

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Large number of high quality software and database programming titles from O'Reilly. Other software titles are also available from Sams and Prentice Hall. Limited to 7 concurrent users.Vast collection of software and database programming titles from multiple publishers, including Microsoft Press.Largest provider of engineering-related eBooks; includes titles in computer science and biomedical engineering.Over 4,000 full-text e-books covering scientific and technical information from CRC Press and others. Many handbooks and single volume reference sources.Includes peer-reviewed life science and biomedical research protocols compiled from Methods in Molecular Biology, Methods in Molecular Medicine, Methods in Biotechnology, Methods in Pharmacology and Toxicology, Neuromethods, the Biomethods Handbook, the Proteomics Handbook, and Springer Laboratory Manuals.Contains full text access to selected biomedical and nursing books.

Provides online, full-text access to Springer's journal titles as well as journals from other publishers.

Subjects include: life sciences, chemical sciences, environmental sciences, geosciences, computer science, mathematics, medicine, physics and astronomy, engineering and economics. Also includes eBooks.

Collection of over 8 thousand fulltext titles in engineering, math, and basic and applied biomedical research. Coverage is from 1967 to the present.A library of ebooks on a wide array of topics, digitized and made available online in conjunction with the original publishers.