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  • Studying NB-LRR immune receptor localization by agroinfiltration transient expression / Patrick Cournoyer and S.P. Dinesh-Kumar -- Fragment complementation and co-immunoprecipitation assays for understanding R protein structure and function / Peter Moffett -- Purification of resistance protein complexes using a biotinylated affinity (HPB) tag / Yiping Qi and Fumiaki Katagiri -- Biochemical purification of native immune protein complexes / James M. Elmore and Gitta Coaker -- Chromatin immunoprecipitation to identify global targets of WRKY transcription factor family members involved in plant immunity / Mario Roccaro and Imre E. Somssich -- Dose-response to and systemic movement of dexamethasone in the GVG-inducible transgene system in Arabidopsis / Xueqing Geng and David Mackey -- Quantifying alternatively spliced mRNA via capillary electrophoresis / Xue-Cheng Zhang and Walter Gassmann -- Constructing haustorium-specific cDNA libraries from rust fungi / Ann-Maree Catanzariti [and others] -- Microaspiration of esophageal gland cells and cDNA library construction for identifying parasitism genes of plant-parasitic nematodes / Richard S. Hussey, Guozhong Huang, and Rex Allen -- Construction of Pseudomonas syringae pv. tomato DC3000 mutant and polymutant strains / Brian H. Kvitko and Alan Collmer -- A straightforwward protocol for electro-transformation of Phytophthora capsici zoospores / Edgar Huitema, Matthew Smoker, and Sophien Kamoun -- Propagation, storage, and assays with Hyaloperonospora arabidopsidis : a model oomycete pathogen of Arabidopsis / John M. McDowell [and others] -- Assaying effector function in planta using double-barreled particle bombardment / Shiv D. Kale and Brett M. Tyler -- Assays for effector-mediated suppression of programmed cell death in yeast / Yuanchao Wang and Qian Huang -- Purification in effector-target protein complexes via transient expression in Nicotiana benthamiana / Joe Win, Sophien Kamoun, and Alexandrea M.E. Jones -- Imaging fluorescently tagged Phytophthora effector proteins inside infected plant tissue / Petra C. Boevink, Paul R.J. Birch, and Stephen C. Whisson -- Immunolocalization of pathogen effectors / Eric Kemen, Kurt Mendgen, and Ralf T. Voegele -- Laser capture microdissection of nematode feeding cells / Nagabhushana Ithal and Melissa G. Mitchum -- Laser microdissection of plant-fungus interaction sites and isolation of RNA for downstream expression profiling / Divya Chandran, Noriko Inada, and Mary C. Wildermuth -- Global expression profiling of RNA from laser microdissected cells at fungal-plant interaction sites / Divya Chandran, Greg Hather, and Mary C. Wildermuth -- Visualizing cellular dynamics in plant-microbe interactions using fluorescent-tagged proteins / William Underwood, Serry Koh, and Shauna C. Somerville.
  • Plant lipid signaling protocols 2013
    Springer Protocols
    As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.
  • Plant metabolomics 2006
  • Roles and functions of plant mineral nutrients / Frans J.M. Maathuis and Eugene Diatloff -- Plant growth and cultivation / Dorina Podar -- Protocols for growing plant symbioses: mycorrhiza / Michael Schultze -- Protocols for growing plant symbioses: rhizobia / Benjamin Gourion, Marie Bourcy, Viviane Cosson, and Pascal Ratet -- Plant cell suspension cultures / Roberto Moscatiello, Barbara Baldan, and Lorella Navazio -- Soil analysis using visible and near infrared spectroscopy / Johanna Wetterlind, Bo Stenberg, and Raphael A. Viscarra Rossel -- Mineral composition analysis: measuring anion uptake and anion concentrations in plant tissues / Malcolm J. Hawkesford, Saroj Parmar, and Peter Buchner -- Multielement plant tissue analysis using ICP spectrometry / T.H. Hansen, T.C. de Bang, K.H. Laursen, P. Pedas, S. Husted, and J.K. Schjoerring -- Mapping element distributions in plant tissues using synchrotron X-ray fluorescence techniques / Erica Donner, Martin D. de Jonge, Peter M. Kopittke, and Enzo Lombi -- Flux measurements of cations using radioactive tracers / D.T. Britto and H.J. Kronzucker -- Ion flux measurements using the MIFE technique / Sergey Shabala, Lana Shabala, Jayakumar Bose, Tracey Cuin, and Ian Newman -- Sampling and analysis of phloem sap / Sylvie Dinant and Julia Kehr -- Methods for xylem sap collection / M. Alexou and A.D. Peuke -- Plant single cell sampling / Wieland Fricke -- Measurements of cytosolic ion concentrations in live cells / Veena S. Anil, Kavitha P.G., Sam Kuruvilla, Pavan Kumar, and M.K. Mathew -- Real time measurement of cytoplasmic ions with ion-selective microelectrodes / Anthony J. Miller -- Large-scale plant ionomics / John M.C. Danku, Brett Labner, Elena Yakubova, and David E. Salt -- Applications of high-throughput plant phenotyping to study nutrient use efficiency / Bettina Berger, Bas de Regt, and Mark Tester.
  • The tomato leaf as a model system for organogenesis -- Usefulness of Physcomitrella patens for studying plant organogenesis -- The dicot root as a model system for studying organogenesis -- Genetic control of root organogenesis in cereals -- Gene expression analysis of aquatic angiosperms podostemaceae to gain insight into the evolution of their enigmatic morphology -- Brown algae as a model for plant organogenesis -- Microscopic analysis of ovule development in Arabidopsis thaliana -- Imaging of phenotypes, gene expression, and protein localization during embryonic root formation in Arabidopsis -- Inducible system for lateral roots in Arabidopsis thaliana and maize -- Adventitious root induction in Arabidopsis thaliana as a model for in vitro root organogenesis -- High-throughput, noninvasive imaging of root systems -- Small-molecule screens to study lateral root development -- Cell lineage analyses in living tissues -- Protein immunolocalization in maize tissues -- Auxin immunolocalization in plant tissues -- Gene expression analysis of shoot apical meristem cell types -- Kinematic analysis of cell division in leaves of mono- and dicotyledonous species: A basis for understanding growth and developing refined molecular sampling strategies -- Regeneration in arabidopsis tissue culture -- Isolation and analysis of mRNAs from specific cell types of plants by ribosome immunopurification -- Analyzing small and long RNAs in plant development using non-radioactive in situ hybridization -- Analyzing protein distribution in plant tissues using "Whole-mount" Immunolocalization -- Culture methods and mutant generation in the filamentous brown algae Ectocarpus siliculosus -- Building simulation models of developing plant organs using VirtualLeaf.
  • v. 1. The Background.
  • Plant proteomics 2007
  • Total protein extraction with TCA-acetone / Valérie Méchin, Catherine Damerval, and Michel Zivy -- Phenol extraction of proteins for proteomic studies of recalcitrant plant tissues / Mireille Faurobert, Esther Pelpoir, and Jamila Chaïb -- Protein extraction from cereal seeds / Gérard Branlard and Emmanuelle Blancel -- Protein extraction from xylem and phloem sap / Julia Kehr and Martijn Rep -- Protein extraction from woody plants / Christophe Plomion and Céline Lalanne -- Isolation of chloroplast proteins from Arabidopsis thaliana for proteome analysis / Klaas J. van Wijk, Jean-Benoit Peltier, and Lisa Giacomelli -- Isolation and subfractionation of plant mitochondria for proteomic analysis / Holger Eubel, Joshua L. Heazlewood, and A. Harvey Millar -- Extraction of nuclear proteins from root meristematic cells / Fernando González-Camacho and Francisco Javier Medina -- Isolation of nuclear proteins / Setsuko Komatsu -- Isolation of cell wall proteins from Medicago sativa stems / Bonnie S. Watson and Lloyd W. Sumner -- Plant plasma membrane protein extraction and solubilization for proteomic analysis / Véronique Santoni -- Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis / Thierry Rabilloud ... [et al.] -- Two-dimensional electrophoresis for plant proteomics / Walter Weiss and Angelika Görg -- Visible and fluorescent staining of two-dimensional gels / François Chevalier, Valérie Rofidal, and Michel Rossignol -- Two-dimensional differential in-gel electrophoresis (DIGE) of leaf and roots of Lycopersicon esculentum / Matthew Keeler ... [et al.] -- Quantitative analysis of 2D gels / Michel Zivy -- Multivariate data analysis of proteome data / Kåre Engkilde, Susanne Jacobsen, and Ib Søndergaard -- Edman sequencing of plant proteins from 2D gels / Setsuko Komatsu -- Peptide mass fingerprinting: identification of proteins by MALDI-TOF / Nicolas Sommerer, Delphine Centeno, and Michel Rossignol -- Protein identification using nano liquid chromatography-tandem mass spectrometry / Luc Negroni -- Two-dimensional nanoflow liquid chromatography-tandem mass spectrometry of proteins extracted from rice leaves and roots / Linda Breci and Paul A. Haynes -- Separation, identification, and profiling of membrane proteins by GFC/IEC/SDS-PAGE and MALDI TOF MS / Wojciech Szponarski ... [et al.] -- The PROTICdb database for 2-DE proteomics / Olivier Langella, Michel Zivy, and Johann Joets -- Identification of phosphorylated proteins / Maria V. Turkina and Alexander V. Vener -- Plant proteomics and glycosylation / Anne-Catherine Fitchette ... [et al.] -- Blue-native gel electrophoresis for the characterization of protein complexes in plants / Jesco Heinemeyer, Dagmar Lewejohann, and Hans-Peter Braun -- Electroelution of intact proteins from SDS-PAGE gels and their subsequent MALDI-TOF MS analysis / Zhentian Lei ... [et al.] -- Generation of plant protein microarrays and investigation of antigen-antibody interactions / Birgit Kersten and Tanja Feilner -- Phosphorylation studies using plant protein microarrays / Tanja Feilner and Birgit Kersten.
  • Analysis of High-Throughput Sequencing Data / Shrinivasrao P. Mane, Thero Modise, Bruno W. Sobral -- Identification of Plant microRNAs Using Expressed Sequence Tag Analysis / Taylor P. Frazier, Baohong Zhang -- Microarray Data Analysis / Saroj K. Mohapatra, Arjun Krishnan -- Setting Up Reverse Transcription Quantitative-PCR Experiments / Madana M. Ambavaram, Andy Pereira -- Virus-Induced Gene Silencing in Nicotiana benthamiana and Other Plant Species / Andrew Hayward, Meenu Padmanabhan, S. P. Dinesh-Kumar -- Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses / Zarir Vaghchhipawala, Clemencia M. Rojas, Muthappa Senthil-Kumar, Kirankumar S. Mysore -- Full-Length cDNA Overexpressor Gene Hunting System (FOX Hunting System) / Mieko Higuchi, Youichi Kondou, Takanari Ichikawa, Minami Matsui -- Activation Tagging with En/Spm-I /dSpm Transposons in Arabidopsis / Nayelli Marsch-Martínez, Andy Pereira -- Activation Tagging and Insertional Mutagenesis in Barley / Michael A. Ayliffe, Anthony J. Pryor -- Methods for Rice Phenomics Studies / Chyr-Guan Chern, Ming-Jen Fan, Sheng-Chung Huang, Su-May Yu, Fu-Jin Wei, Cheng-Chieh Wu, Arunee Trisiriroj, Ming-Hsing Lai, Shu Chen, Yue-Ie C. Hsing -- Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice / Sung-Ryul Kim, Jong-Seong Jeon, Gynheung An -- Transposon Insertional Mutagenesis in Rice / Narayana M. Upadhyaya, Qian-Hao Zhu, Ramesh S. Bhat -- Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants / Xiaofei Cheng, Jiangqi Wen, Million Tadege, Pascal Ratet, Kirankumar S. Mysore -- Screening Arabidopsis Genotypes for Drought Stress Resistance / Amal Harb, Andy Pereira -- Protein Tagging for Chromatin Immunoprecipitation from Arabidopsis / Stefan Folter -- Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions / Pieter B. Ouwerkerk, Annemarie H. Meijer -- Plant Metabolomics by GC-MS and Differential Analysis / Joel L. Shuman, Diego F. Cortes, Jenny M. Armenta, Revonda M. Pokrzywa, Pedro Mendes, Vladimir Shulaev -- Gramene Database: A Hub for Comparative Plant Genomics / Pankaj Jaiswal.
  • Plant secondary metabolites 2007
    Springer Protocols
  • Multiple dimensions in plant signal transduction : an overview / Jorge J. Casal -- Phenotyping of Arabidopsis mutants for developmental effects of gene deletions / Cordelia Bolle -- Phenotyping of abiotic responses and hormone treatment in Arabidopsis / Cordelia Bolle -- Accurate real-time reverse transcription quantitative PCR / Marco Klatte and Petra Bauer -- Probing spatio-temporal intracellular calcium variations in plants / Axel Mithöfer, Christian Mazars, and Massimo E. Maffei -- Dynamic redox measurements with redox-sensitive GFP in plants by confocal laser scanning microscopy / Andreas J. Meyer and Thorsten Brach -- Imaging reactive oxygen species in vivo / Steven M. Driever ... [et al.] -- Identification of thioredoxin targeted proteins using thioredoxin single cysteine mutant-immobilized resin / Ken Motohashi, Patrick G.N. Romano, and Toru Hisabori -- Determination of in vivo protein phosphorylation in photosynthetic membranes / Julia P. Vainonen, Alexander V. Vener, and Eva-Mari Aro -- Examining protein stability and its relevance for plant growth and development / Claus Schwechheimer ... [et al.] -- Probing circadian rhythms in Chlamydomonas rheinhardtii by functional proteomics / Volker Wagner and Maria Mittag -- Bimolecular fluorescence complementation (BiFC) to study protein-protein interactions in living plant cells / Katia Schütze, Klaus Harter, and Christina Chaban -- Fluorescence cross-correlation spectroscopy of plant proteins / Hideki Muto, Masataka Kinjo, and Kotaro T. Yamamoto -- The determination of protein-protein interactions by the mating-based split-ubiquitin-system (mbSUS) / Christopher Grefen, Petr Obrdlik, and Klaus Harter -- Functional complementation of yeast mutants to study plant signalling pathways / Norbert Mehlmer, Elisabeth Scheikl-Pourkhalil, and Markus Teige -- Phosphatase activities analyzed by in vivo expressions / Alois Schweighofer, Zahra Ayatollahi, and Irute Meskiene -- Chromatin immunoprecipitation experiments to investigate in vivo binding of Arabidopsis transcription factors to target sequences / Benjamin Fode and Christiane Gatz -- Fluorescence-based electrophoretic mobility shift assay in the analysis of DNA-binding proteins / Sebastian Steiner and Thomas Pfannschmidt -- Analysis of plant regulatory DNA sequences by the yeast-one-hybrid assay / Dierk Wanke and Klaus Harter -- Analysis of plant regulatory DNA sequences by transient protoplast assays and computer aided sequence evaluation / Kenneth W. Berendzen, Klaus Harter, and Dierk Wanke -- RNA protein interaction mediating post-transcriptional regulation in the circadian system / Jan C. Schöning and Dorothee Staiger.
  • Transposable elements are DNA sequences with the capacity to move within a genome. Although their presence and impact has long been known, the recent genome-wide analysis of many eukaryotic genomes has uncovered their major role in genome dynamics and function. The present book explains how to recognize and study transposable elements, e.g. by using state-of-the-art strategies based on new-generation sequencing. Moreover, the impact of transposable elements on plant genome structure and function is reviewed in detail and illustrated in examples and case studies. The book is intended both for readers familiar with the field and for newcomers. With large-scale sequencing becoming increasingly available, more and more people will come across transposable element sequences in their data, and this volume will hopefully help to convince them that transposable elements are not just "junk" DNA, and may actually be the most interesting and fun part of their data!
  • Plant viral vectors 2014
    In this volume, the authors provide an excellent overview of how far the plant viral vector field has come. The discipline is no longer exclusively in the domain of academics-there is a small, but growing number of small biotechnology companies that exploit plant viruses as the platform for commercial innovation in crop improvement, industrial product manufacturing, and human and veterinary health care.
  • Plant-derived vaccines : technologies & applications / Franco M. Buonaguro -- Patents and molecular farming / Pascal M.W. Drake & Harry Thangaraj -- In planta production of recombinant and indigenous adjuvants / Antonio Granell, Asun Fernandez-del-Carmen & Diego Orzáez -- Rapid and high-yield production of immunotherapeutic plant-made antibodies / Cristina Capodicasa, Marcello Donini, Maria Elena Villani & Eugenio Benvenuto -- Vaccine peptide display on recombinant tobacco mosaic virus particles / Tatiana V. Komarova, Igor V. Petrunia & Yuri L. Dorokhov -- Plastid transformation as a tool for plant-based production of vaccines and therapeutic proteins / Paolo Lenzi, Nunzia Scotti, Pal Maliga & Teodoro Cardi -- Prophylactic and therapeutic HPV vaccines from plants / Edward P. Rybicki, Colomba Giorgi & Rosella Franconi -- Plant-derived anti-HIV-1 proteins / Nunzia Scotti, Luigi Buonaguro, Maria Lina Tornesello, Teodoro Cardi & Franco Maria Buonaguro -- Plant-based hepatitis B vaccines / Sergei N. Shchelkunov & Galina A. Shchelkunova -- Influenza vaccines : new perspectives from plants / Chiara Lico, Giampaolo Buriani, Floriana Capuano, Eugenio Benvenuto & Selene Baschieri -- Plant-made mucosal vaccines / Rurick K. Salyaev, Maria Manuela Rigano & Natalya I. Rekoslavskaya -- Current status of plant-made veterinary vaccines / Assunta Pelosi, Claire Penney, Huai-Yian Ling & Amanda M. Walmsley.
  • The use of protoplasts to study innate immune responses / Ping He, Libo Shan, and Jen Sheen -- Marker-exchange mutagenesis and complementation strategies for the gram-negative bacteria Xanthomonas oryzae pv. oryzae / Sang-Won Lee and Pamela C. Ronald -- Whole-genome analysis to identify type III-secreted effectors / Boris A. Vinatzer and Jean T. Greenberg -- In planta expression of oomycete and fungal genes / Thirumala-Devi Kanneganti, Edgar Huitema, and Sophien Kamoun -- Use of nipponbare bac clones for physical mapping of an R gene locus in rice / Jong-Seong Jeon and Pamela C. Ronald -- Agrobacterium-mediated transformation to create an insertion library in magnaporthe grisea / Sara L.Tucker and Marc J. Orbach -- Identification of components in disease-resistance signaling in arabidopsis by map-based cloning / Yuelin Zhang, Jane Glazebrook, and Xin Li -- Yeast two-hybrid approaches to dissecting the plant defense response / Mawsheng Chern, Todd Richter, and Pamela C. Ronald -- Use of rolling-circle amplification for large-scale yeast two-hybrid analyses / Xiaodong Ding, Yan Zhang, and Wen-Yuan Song -- Preparative denaturing isoelectric focusing for enhancing sensitivity of proteomic studies / Antonio Serna-Sanz, Greg Rairdan, and Scott C. Peck -- Use of massively parallel signature sequencing to study genes expressed during the plant defense response / Blake C. Meyers, Christian D. Haudenschild, and Kalyan Vemaraju -- Use of microarray analysis to dissect the plant defense response / Jane Glazebrook -- Use of robust long-serial analysis of gene expression to identify novel fungal and plant genes involved in host-pathogen interactions / Malali Gowda ... [et al.] -- Analysis of gene function in rice through virus-induced gene silencing / Xin Shun Ding, C. Srinivasa Rao, and Richard S. Nelson -- Use of RNA interference to dissect defense-signaling pathways in rice / Chuansheng Mei, Xiangjun Zhou, and Yinong Yang -- Virus-induced gene silencing in plant roots / Isgouhi Kaloshian -- Methods for engineering resistance to plant viruses / Mysore R. Sudarshana, Gourgopal Roy, and Bryce W. Falk -- Development of genetically engineered resistant papaya for papaya ringspot virus in a timely manner: a comprehensive and successful approach / Savarni Tripathi, Jon Suzuki, and Dennis. Gonsalves.
  • Also available: Print – 2009
    Plant vaccines: an immunological perspective / D.C. Hooper -- Display of peptides on the surface of tobacco mosaic virus particles / M.L. Smith ... [et al.] -- Chloroplast-derived vaccine antigens and biopharmaceuticals: expression, folding, assembly and functionality / S. Chebolu and H. Daniell -- Production of antibodies in plants: approaches and perspectives / K. Ko, R. Brodzik and Z. Steplewski -- Plant production of veterinary vaccines and therapeutics / R.W. Hammond and L.G. Nemchinov -- Plant-based oral vaccines: results of human trials / C.O. Tacket.
  • Pomegranate phytochemicals / Navindra P. Seeram ... [et al.] -- Antioxidative properties of pomegranate : in vitro studies / Mira Rosenblat and Michael Aviram -- Bioavailability of pomegranate polyphenols / Francisco A. Tomas-Barberan, Navindra P. Seeram, and Juan Carlos Espin -- Protection against cardiovascular disease / Bianca Fuhrman and Michael Aviram -- Protection against stroke / Marva I. Sweeney-Nixon -- Anticancer potential of pomegranate / Shishir Shishodia ... [et al.] -- Molecular mechanisms of chemoprevention of cancer by pomegranate / Deeba Syed ... [et al.] -- Pomegranate and prostate cancer chemoprevention / John T. Leppert and Allan J. Pantuck -- Accessment of estrogenicity of pomegranate in an in vitro bioassay / Diane M. Harris, Emily Besselink, and Navindra P. Seeram -- Absence of significant estrogenic effects in the postmenopausal population / Michelle P. Warren ... [et al.] -- Antimicrobial activities of pomegranate / G.K. Jayaprakasha, P.S. Negi, B.S. Jena -- Commercialization of pomegranates : fresh fruit, beverages, and botanical extracts / Navindra P. Seeram, Yanjun Zhang, and David Heber -- Pomegranates: a botanical perspective / David W. Still -- Postharvest biology and technology of pomegranates / Adel A. Kader.
  • Machine generated contents note: pt. ONE Background and strategies -- 1.Herbal therapeutic systems -- 2.Principles of herbal pharmacology -- 3.Principles of herbal treatment -- 4.Validating herbal therapeutics -- 5.Optimising safety -- pt. TWO Practical clinical guides -- 6.Dosage and dosage forms in herbal medicine -- 7.A systematic approach to herbal prescribing -- 8.Herbal approaches to pathological states -- Topical applications -- Fever -- Infectious diseases -- Inflammatory and autoimmune diseases -- Fatigue and debility -- Malignant diseases -- 9.Herbal approaches to system dysfunctions -- Digestive system and bowel -- Biliary system -- The liver -- Cardiovascular system -- Respiratory system -- Urinary system -- Nervous system -- Female reproductive system -- Joint diseases -- Skin diseases -- Male reproductive system -- Endocrine disorders -- pt. THREE Materia Medica -- 10.How to use the monographs -- Andrographis -- Arnica flowers -- Astragalus -- Bearberry -- Contents note continued: Berberis bark and Hydrastis root -- Bilberry fruit -- Black cohosh -- Boswellia -- Buchu -- Bugleweed and European bugleweed -- Bupleurum -- Butcher's broom -- Chamomile, German -- Chaste tree -- Chelidonium -- Devil's claw -- Dong quai -- Echinacea root -- Evening primrose oil -- Eyebright -- Fennel fruit -- Feverfew -- Ginger -- Ginkgo -- Ginseng -- Globe artichoke -- Gotu kola -- Hawthorn -- Horsechestnut seed -- Kava -- Licorice -- Meadowsweet -- Melilotus -- Myrrh -- Nettle -- Pau d'arco -- Peppermint -- Poke root -- Rehmannia -- Saw palmetto -- Siberian ginseng -- St John's wort -- St Mary's thistle -- Thyme -- Tribulus leaf -- Turmeric -- Valerian -- Willow bark -- Witchhazel -- Withania.
  • Establishment of adventitious root cultures of Echinacea purpurea for the production of caffeic acid derivatives / K.Y. Paek, H.N. Murthy and E.J. Hahn -- In vitro propagation of Rauwolfia serpentina using liquid medium, assessment of genetic fidelity of micropropagated plants, and simultaneous quantitation of reserpine, ajmaline, and ajmalicine / M.K. Goel ... [et al.] -- Medicinal properties, in vitro protocols and secondary metabolite analyses of Scots pine / H. Haggman ... [et al.] -- Saussurea medusa cell suspension cultures for flavonoid production / C.Z. Liu and P.K. Saxena -- Large-scale in vitro multiplication of Crataeva nurvala / S.B. Babbar, N. Walia and A. Kaur -- Bilberry in vitro protocols and analyses of phenolic compounds / L. Jaakola ... [et al.] -- In vitro propagation of two tuberous medicinal plants: Holostemma ada-kodien and Ipomoea mauritiana / S.P. Geetha ... [et al.] -- In vitro production of gymnemic acid from Gymnema sylvestre (Retz) R. Br. Ex Roemer and Schultes through callus culture under abiotic stress conditions / A.B. Ali Ahmed, A.S. Rao and M.V. Rao -- Establishment of plant regeneration and cryopreservation system from zygotic embryo-derived embryogenic cell suspension cultures of Ranunculus kazusensis / S.W. Kim and M.J. Oh -- In vitro culture and secondary metabolite isolation in bryophytes / A. Sabovljevic, M. Sabovljevic and N. Jockovic -- Micropropagation and in vitro conservation of vanilla (Vanilla planifolia Andrews) / M. Divakaran and K.N. Babu -- Protocol for in vitro regeneration and marker glycoside assessment in Swertia chirata Buch-Ham / S. Koul ... [et al.] -- Protocols for establishment of an in vitro collection of medicinal plants in the genus Scutellaria / I.B. Cole, F.T. Farooq and S.J. Murch -- Protocols for in vitro culture and phytochemical analysis of Phyllanthus species (Euphorbiaceae) / E. Catapan ... [et al.] -- In vitro clonal propagation of Asparagus racemosus, a high value medicinal plant / S. Saxena and N. Bopana -- Micropropagation of Penthorum chinense through axillary buds / J. Yang and Z.S. Peng -- Spontaneous plant regeneration and production of secondary metabolites from hairy root cultures of Centaurium erythraea Rafn / A. Subotic ... [et al.] -- Transgenic Hypericum perforatum / G. Franklin, M.M. Oliveira and A.C. Dias -- Agrobacterium-mediated transformation of Ruta graveolens L / K. Lievre ... [et al.] -- Gene expression profiling in Taxus baccata L. seedlings and cell cultures / K. Brun akova and J. Kosuth -- Catapol production in Chinese foxglove (Rehmannia glutinosa Libos.) hairy roots transformed with Agrobacterium rhizogenes ATCC15834 / S.J. Hwang -- Identification of medicinal plants and plant sequences: multiplexed MLPA assay / R.A. Barthelson -- Isolation of microsatellites from Catharanthus roseus (L.) G. Don using enriched libraries / S. Bhatia and B. Shokeen -- Production of cinnamyl glycosides in compact callus aggregate cultures of Rhodiola rosea through biotransformation of cinnamyl alcohol / Z. Gyorgy and A. Hohtola -- Spearmint plantlet culture system as a means to study secondary metabolism / B. Tisserat, M. Berhow and S.F. Vaughn -- Bioreactor production of secondary metabolites from cell cultures of periwinkle and sandalwood / J.V. Valluri -- Camptothecin production by in vitro cultures and plant regeneration in Ophiorrhiza species / T. Asano ... [et al.] -- Metabolomic analysis of Ocotea odorifera cell cultures: a model protocol for acquiring metabolite data / M. Maraschin ... [et al.] -- Production of 9-methoxycanthin-6-one from callus cultures of (Eurycoma longifolia Jack) Tongkat Ali / M. Maziah and N. Rosli -- Plant secondary metabolism in altered gravity / L.K. Tuominen, L.H. Levine and M.E. Musgrave -- The role of biotechnology in the production of the anticancer compound podophyllotoxin / H. Lata, C.S. Mizuno and R.M. Moraes.
  • From Neanderthal to nanobiotech : from plant potions to pharming with plant factories / Christophe Sourrouille ... [et al.] -- Cowpea mosaic virus-based systems for the expression of antigens and antibodies in plants / Frank Sainsbury, Li Liu, and George P. Lomonossoff -- Transient expression of antibodies in plants using syringe agroinfiltration / Marc-André D'Aoust ... [et al.] -- Rapid system for evaluating bioproduction capacity of complex pharmaceutical proteins in plants / Giuliana Medrano ... [et al.] -- Production and localization of recombinant pharmaceuticals in transgenic seeds / Thomas Rademacher, Elsa Arcalis, and Eva Stoger -- Production of antibody fragments in Arabidopsis seeds / Bart Van Droogenbroeck, Kirsten De Wilde, and Ann Depicker -- Production of plantibodies in Nicotiana plants / Marta Ayala ... [et al.] -- Physcomitrella patens : a non-vascular plant for recombinant protein production / David Liénard and Fabien Nogué -- Production of recombinant proteins in suspension-cultured plant cells / Carole Plasson ... [et al.] -- Chloroplast-derived vaccine antigens and biopharmaceuticals : protocols for expression, purification, or oral delivery and functional evaluation / N. Dolendro Singh, Yi Ding, and Henry Daniell -- Protein body induction : a new tool to produce and recover recombinant proteins in plants / Margarita Torrent, Imma Llop-Tous, and M. Dolors Ludevid -- A case study for plant-made pharmaceuticals comparing different plant expression and production systems / Guy Vancanneyt ... [et al.] -- Glycosylation of antibody therapeutics : optimisation for purpose / Roy Jefferis -- N-glycosylation of plant recombinant pharmaceuticals / Muriel Bardor ... [et al.] -- Companion protease inhibitors to protect recombinant proteins in transgenic plant extracts / Meriem Benchabane ... [et al.] -- Strategies for improving vaccine antigens expression in transgenic plants : fusion to carrier sequences / Jose M. Escribano and Daniel M. Perez-Filgueira -- Immunomodulation of plant function by in vitro selected single-chain Fv intrabodies / Manfred Gahrtz and Udo Conrad -- On-chip detection of low-molecular-weight recombinant proteins in plant crude extracts by SELDI-TOF MS / Amine M. Badri ... [et al.] -- Assessing the risk of undesirable immunogenicity/allergenicity of plant-derived therapeutic proteins / Paul D. Chamberlain -- Biosafety, risk assessment, and regulation of plant-made pharmaceuticals / Penelope A.C. Sparrow and Richard M. Twyman.
  • Rhodiola rosea 2014
    Chapter 1. Taxonomy of Rhodiola rosea L., with special attention to molecular analyses of Nunavik (Québec) populations / Alain Cuerrier, Mariannick Archambault, Michel Rapinski, and Anne Bruneau -- Chapter 2. Ethnobotany and conservation of Rhodiola species / Alain Cuerrier, Youri Tendland, and Michel Rapinski -- Chapter 3. Phytochemistry of Rhodiola rosea / Fida Ahmed, Vicky Filion, Ammar Saleem, and John T. Arnason -- Chapter 4. Cultivation of Rhodiola rosea in Europe / Bertalan Galambosi -- Chapter 5. Rhodiola rosea cultivation in Canada and Alaska / Kwesi Ampong-Nyarko -- Chapter 6. Diseases of wild and cultivated Rhodiola rosea / Sheau-Fang Hwang, Stephen E. Strelkov, Kwesi Ampong-Nyarko, and Ron J. Howard -- Chapter 7. Biotechnology of Rhodiola rosea / Zsuzsanna György -- Chapter 8. Pharmacological activities of Rhodiola rosea / Fida Ahmed, Steffany A.L. Bennett, and John T. Arnason -- Chapter 9. Evidence-based efficacy and effectiveness of Rhodiola SHR-5 extract in treating stress- and age- associated disorders / Alexander Panossian and Georg Wikman -- Chapter 10. Rhodiola rosea in psychiatric and medical practice / Patricia L. Gerbarg, Petra A. Illig, and Richard P. Brown -- Chapter 11. Toxicology and safety of Rhodiola rosea / Hugh Semple and Brandie Bugiak -- Chapter 12. Commercialization of Rhodiola rosea / Nav Sharma and Raimar Loebenberg.
  • Rice protocols 2013
    Springer Protocols
    Rice artificial hybridization for genetic analysis / Xueyan Sha -- Fluorescence in situ hybridization techniques for cytogenetic and genomic analyses / Jason G. Walling, Wenli Zhang, and Jiming Jiang -- Generation of rice mutants by chemical mutagenesis / Thomas H. Tai -- TILLING and ecotilling for rice / Jennifer L. Cooper [and others] -- Functional characterization of rice genes using a gene-indexed T-DNA insertional mutant population / Ki-Hong Jung and Gynheung An -- QTL analysis and map-based cloning of salt tolerance gene in rice / Ji-Ping Gao and Hong-Xuan Lin -- Site-specific gene integration in rice / Vibha Srivastava -- Transcriptome profiling analysis using rice oligonucleotide microarrays / Ki-Hong Jung and Pamela C. Ronald -- Cloning of small RNAs for the discovery of novel microRNAs in plants / Guru Jagadeeswaran and Ramanjulu Sunkar -- Global identification of small RNA targets in plants by sequencing sliced ends of messenger RNAs / Yong-Fang Li and Ramanjulu Sunkar -- Artificial microRNAs for specific gene silencing in rice / Norman Warthmann [and others] -- Rice proteomic analysis : sample preparation for protein identification / Ganesh Kumar Agrawal [and others] -- Quantification of jasmonic and salicylic acids in rice seedling leaves / Kyoungwon Cho [and others] -- Analysis of insect-induced volatiles from rice / Nan Zhao [and others] -- Phenotypic and physiological evaluation for drought and salinity stress responses in rice / Utlwang Batlang [and others] -- Phenotypic, physiological, and molecular evaluation of rice chilling stress response at the vegetative stage / Benildo G. de los Reyes [and others] -- Analysis of rice root hair morphology using cryo-scanning electron microscopy / Zhiming Yu -- Inoculation and virulence assay for bacterial blight and bacterial leaf streak of rice / Bing Yang and Adam Bogdanove -- Inoculation and scoring methods for rice sheath blight disease / Yulin Jia [and others] -- Molecular approaches to improve rice abiotic stress tolerance / Junya Mizoi and Kazuko Yamaguchi-Shinozaki -- Molecular strategies to improve rice disease resistance / Emily E. Helliwell and Yinong Yang -- Molecular strategies to engineer transgenic rice seed compartments for large-scale production of plant-made pharmaceuticals / Trevor Greenham and Illimar Altosaar.
  • Root genomics 2011
    With the predicted increase of the human population and the subsequent need for larger food supplies, root health in crop plants could play a major role in providing sustainable highly productive crops that can cope with global climate changes. While the essentiality of roots and their relation to plant performance is broadly recognized, less is known about their role in plant growth and development. "Root Genomics" examines how various new genomic technologies are rapidly being applied to the study of roots, including high-throughput sequencing and genotyping, Tilling, transcription.
  • Shengmai san 2002
    1. Shengmai San - a renowned traditional Chinese medicinal formula / Song-Ming Liang, Shu-Ying Chen, Si-Qian Liang -- 2. Pharmacological studies on Shengmai San / Kam-Ming Ko, Duncan H.F. Mak, Tze-Kin Yim and Yong-Quing Yan -- 3. Clinical studies on Shengmai San / Ye-Zhi Rong, Mei-Hua Zhao, Bao-Jing Lu, Xiang-Yang Zhu, Shang-Biao Lu, Ya-Chen Zhang, Jie Chen -- 4. Side effects of Shengmai San / Zhi-Min Xu and Ye-Zhi Rong -- 5. Phytochemistry and pharmacology of component herbs of Shengmai San / Siu-Po Ip, Timothy C.M. Tam and Chung-Tao Che -- 6. Contemporary applications of a traditional formula: manufacture of Shengmai San (SMS) preparations and their applications / Liang-Yuan Zheng -- Glossary.
  • This edited volume addresses a rising concern among natural resource scientists and management professionals about decline of the many plant and animal species associated with early-successional habitats, especially within the Central Hardwood Region of the USA. These open habitats, with herbaceous, shrub, or young forest cover, are disappearing as abandoned farmland, pastures, and cleared forest patches return to forest. There are many questions about 'why, what, where, and how' to manage for early successional habitats. In this book, expert scientists and experienced land managers synthesize knowledge and original scientific work to address critical questions sparked by the decline of early successional habitats.
  • Transgenic cotton : from biotransformation methods to agricultural application / Baohong Zhang -- Genetically modified cotton in India and detection strategies / Gurinder Jit Randhawa and Rashmi Chhabra -- Agrobacterium-mediated transformation of cotton / Baohong Zhang -- Biolistic transformation of cotton zygotic embryo meristem / Kanniah Rajasekaran -- Biolistic transformation of cotton embryogenic cell suspension cultures / Kanniah Rajasekaran -- Cotton transformation via pollen tube pathway / Min Wang, Baohong Zhang, and Qinglian Wang -- Silicon carbide whisker-mediated transformation of cotton (Gossypium hirsutum L.) / Muhammad Arshad, Yusuf Zafar, and Shaheen Asad -- Investigating transgene integration and organization in cotton (Gossypium hirsutum L.) genome / Jun Zhang and Yan Hong -- Estimating the copy number of transgenes in transformed cotton by real-time quantitative PCR / Chengxin Yi and Yan Hong -- Development of enzyme-linked immunosorbent assay for the detection of Bt protein in transgenic cotton / Suchitra Kamle, Abhishek Ojha, and Arvind Kumar -- DNA-based diagnostics for genetically modified cotton : decaplex PCR assay to Differentiate MON531 and MON15985 Bt cotton events / Gurinder Jit Randhawa, Monika Singh, and Rashmi Chhabra -- Simple and rapid method for determining transgenic cotton plants / Baohong Zhang [and others] -- Efficient grafting technique for recovery of transgenic cotton plants / Min Wang, Qinglian Wang, and Baohong Zhang -- Inheritance of transgenes in transgenic Bt lines resistance to Helicoerpa armigera in upland cotton / Baolong Zhang, Wangzhen Guo, and Tianzhen Zhang -- Agrobacterium rhizogenes-induced cotton hairy root culture as an alternative tool for cotton functional genomics / Hee Jin Kim -- Overexpression of miR 156 in cotton via Agrobacterium-mediated transformation / Baohong Zhang [and others] -- Development of transgenic CryIA(c) + GNA cotton plants via pollen tube pathway method confers resistance to Helicoverpa armigera and Aphis gossypii Glover / Zhi Liu, Zhen Zhu, and Tianzhen Zhang -- Agrobacterium-mediated transformation of cotton (Gossypium hirsutum) shoot apex with a fungal phytase gene improves phosphorus acquisition / Zhiying Ma, Jianfeng Liu, and Xingfen Wang -- Genetic transformation of cotton with a harpin-encoding gene hpaXoo confers an enhanced defense response against Verticillium dahliae kleb / Weiguo Miao and Jingsheng Wang -- Development of insect-resistant transgenic cotton with chimeric TVip3A* accumulating in chloroplasts / Jiahe Wu and Yingchuan Tian -- Determining gene flow in transgenic cotton / Xiaoping Pan.
  • Transgenic wheat, barley and oats : production and characterization / Paul A. Lazzeri and Huw D. Jones -- Selection of transformed plants / Huw D. Jones and Caroline A. Sparks -- Reporter genes / Alison Huttly -- Biolistics transformation of wheat / Caroline A. Sparks and Huw D. Jones -- Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos / Huixia Wu, Angela Doherty and Huw D. Jones -- Floral transformation of wheat / Sujata Agarwal ... [et al.] -- Highly efficient agrobacterium-mediated transformation of wheat via in planta inoculation / Thierry Risacher ... [et al.] -- Barley transformation using biolistic techniques / Wendy A. Harwood and Mark A. Smedley -- Barley transformation using agrobacterium-mediated techniques / Wendy A. Harwood ... [et al.] -- Transformation of oats and its application to improving osmotic stress tolerance / Shahina B. Maqbool ... [et al.] -- Promoter sequences for defining transgene expression / Huw D. Jones and Caroline A. Sparks -- Down-regulation of gene expression by RNA-induced gene silencing / Silvia Travella and Beat Keller -- Gene insertion patterns and sites / Philippe Vain and Vera Thole -- Fluorescent in situ hybridization to detect transgene integration into plant genomes / Trude Schwarzacher -- Establishing substantial equivalence : transcriptomics / María Marcela Baudo ... [et al.] -- Establishing substantial equivalence : proteomics / Alison Lovegrove, Louise Salt, and Peter R. Shewry -- Establishing substantial equivalence : metabolomics / Michael H. Beale, Jane L. Ward, and John M. Baker -- Design and management of field trials of transgenic cereals / Zoltán Bedő, Mariann Rakszegi, and László Láng -- GM risk assessment / Penny A.C. Sparrow -- Transgenic wheat, barley and oats : future prospects / Jim M. Dunwell.
  • Fully revised, updated and offered in a new two-volume format, The Yeasts: A Taxonomic Study, 5th Edition remains the most comprehensive presentation of yeast taxonomy and systematics available. Nearly 1500 species of ascomycete and basidiomycete yeasts are included, each description offering not only standard morphological and physiological characters, but also information on systematics, habitat, ecology, agricultural and biotechnological applications and clinical importance. Extensive introductory chapters discuss clinical aspects of yeasts, their role in biotechnology, food and beverage spoilage, agriculture and ecology, while other chapters include methodology for isolation of species from various habitats, phenotypic characterization, chemotaxonomy, gene sequence analysis and phylogenetics, including whole genome analysis. Additionally, easy-to-understand trees illustrate the phylogenetic placement of each species in its assigned genus as they have been determined from gene sequence analysis. This essential work, prepared by the leading experts in the field, is the most definitive treatment of taxonomy and systematics of yeasts on the market, and a necessary reference for any bookshelf or workbench. High quality photomicrographs and line drawings enhance world-class content Detailed trees clearly illustrate phylogenetic placement of each species in its assigned genus Discussion of clinical aspects of yeasts, including their role in biotechnology, agriculture and ecology help contextualize content for a wide range of researchers.

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