Lane Medical Library

Books by Subject

Biotechnology

  • Digital
    Monique Frize.
    Atypon2011
    Increasingly, biomedical scientists and engineers are involved in projects, design, or research and development that involve humans or animals. The book presents general concepts on professionalism and the regulation of the profession of engineering, including a discussion on what is ethics and moral conduct, ethical theories and the codes of ethics that are most relevant for engineers. An ethical decision-making process is suggested. Other issues such as conflicts of interest, plagiarism, intellectual property, confidentiality, privacy, fraud, and corruption are presented. General guidelines, the process for obtaining ethics approval from ethics Review Boards,and the importance of obtaining informed consent from volunteers recruited for studies are presented. A discussion on research with animals is included. Ethical dilemmas focus on reproductive technologies, stem cells, cloning, genetic testing, and designer babies. The book includes a discussion on ethics and the technologies of body enhancement and of regeneration. The importance of assessing the impact of technology on people, society, and on our planet is stressed. Particular attention is given to nanotechnologies, the environment, and issues that pertain to developing countries. Ideas on gender, culture, and ethics focus on how research and access to medical services have, at times, been discriminatory towards women. The cultural aspects focus on organ transplantation in Japan, and a case study of an Aboriginal child in Canada; both examples show the impact that culture can have on how care is provided or accepted. The final section of the book discusses data collection and analysis and offers a guideline for honest reporting of results, avoiding fraud, or unethical approaches. The appendix presents a few case studies where fraud and/or unethical research have occurred.
  • Digital
    Jan Helge Solbakk, Søren Holm, Bjørn Hofmann, editors.
    Springer2009
    Pt. 1. Research biobanking : the traditional approach -- Consent to biobank research : one size fits all? / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- What no one knows cannot hurt you : the limits of informed consent in the emerging world of biobanking / Arthur L. Caplan -- Users and uses of the biopolitics of consent : a study of DNA banks / Pascal Ducournau and Anne Cambon-Thomsen -- Information rights on the edge of ignorance / Anne Maria Skrikerud -- The dubious uniqueness of genetic information / Anne Maria Skrikerud -- Duties and rights of biobank participants : principled autonomy, consent, voluntariness and privacy / Lars Øystein Ursin -- Biobanking and disclosure of research results : addressing the tension between professional boundaries and moral intuition / Lynn G. Dressler -- Biobanks and our common good / Erik Christensen -- Trust, distrust and co-production : the relationship between research biobanks and donors / Pascal Ducournau and Roger Strand -- Scientific citizenship, benefit, and protection in population-based research / Vilhj́almur Árnason -- Pt. 2. Research Biobanking : towards a new conceptual approach -- Mapping the language of research biobanking : an analogical approach / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- The use of analogical reasoning in umbilical cord blood biobanking / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- The Alexandria plan : creating libraries for human tissue research and therapeutic use / Laurie Zoloth -- The art of biocollections / Anne Hambro Alnæs -- The health dugnard : biobank participation as the solidary pursuit of the common good / Lars Øystein Ursin and Berge Solberg -- Embodied gifting : reflections on the role of information in biobank recruitment / Klaus Hoeyer -- Conscription to biobank research? / Bjørn Hofmann, Jan Helge Solbakk, Søren Holm -- Ownership rights in research biobanks : do we need a new king of 'biological property'? / Paula Lobato de Faria -- Legal challenges and strategies in the regulation of research biobanking / Elisabeth Rynning -- Annexation of life : the biopolitics of industrial biology / Jan Reinert Karlsen and Roger Strand -- In the ruins of Babel : should biobank regulations be harmonized / Jan Reinert Karlsen, Jan Helge Solbak, and Roger Strand.
  • Digital/Print
    Steven D. Schwartzbach, Shigeru Shigeoka, editors.
    Digital : Springer2017
    Print2017
    "This much-needed book is the first definitive volume on Euglena in twenty-fire years, offering information on its atypical biochemistry, cell and molecular biology, and potential biotechnology applications. This volume gathers together contributions from well-known experts, who in many cases played major roles in elucidating the phenomenon discussed. Presented in three parts, the first section of this comprehensive book describes novel biochemical pathways which in some instances have an atypical subcellular localization. The second section details atypical cellular mechanisms of organelle protein import, organelle nuclear genome interdependence, gene regulation and expression that provides insights into the evolutionary origins of eukaryotic cells. The final section discusses how biotechnologists have capitalized on the novel cellular and biochemical features of Euglena to produce value added products. Euglena: Biochemistry, Cell and Molecular Biology will provide essential reading for cell and molecular biologists with interests in evolution, novel biochemical pathways, organelle biogenesis and algal biotechnology. Readers will come away from this volume with a full understanding of the complexities of the Euglena as well as new realizations regarding the diversity of cellular processes yet to be discovered"--Publisher's description.
  • Digital
    Peter T. Robbins, Farah Huzair, editors.
    Springer2011
  • Digital/Print
    Digital : WHO2010
    Print2010
    "The advent of nanotechnology has unleashed enormous prospects for the development of new products and applications for a wide range of industrial and consumer sectors. The new technological developments have already opened up a multibillion dollar industry in recent years, the global market impact of which is expected to reach US$1 trillion by 2015, with around 2 million workers. While the majority of manufacturing and use of nanoscale materials occurs in the United States, the European Union, with its around 30 percent global share of the sector, is not lagging far behind in this field. Like other sectors, nanotechnology promises to revolutionize the whole food chain--from production to processing, storage, and development of innovative materials, products and applications. Although the potential applications of nanotechnoloy are wide ranging, the current applications in the food and agricultural sectors are relatively few, because the science is still newly emergent. An overview of more than 800 nanotechnology-based consumer products that are currently available worldwide, suggests that only around 10 percent of these are foods, beverages and food packaging products. However, nanotechnology-derived products and applications in these sectors have been steadily increasing in recent years, and are predicted to grow rapidly in the future. This is because the new technologies have a great potential to address many of the industry's current needs." - p. 3
  • Digital
    Ijeoma F. Uchegbu, Andreas G. Schätzlein, Woei Ping Cheng, Aikaterini Lalatsa, editors.
    Springer2013
    Nanoscience and nanotechnology overview -- Liposome preparation and characterization -- Solid polymeric nanoparticles -- Lipid nanoparticles -- Polymeric vesicles -- Low molecular weight amphiphile micelles -- Polymeric micelles -- Niosomes -- Quantum dots -- Magnetic particles -- Drug nanoparticles -- Polymeric pro-drugs -- Therapeutic Applications of pharmaceutical nanosystems -- Drug solubilisation -- Gene therapy -- Chemotherapy -- Tissue engineering -- Anti-infectives -- Vaccination -- Anti-arthritic drugs -- Imaging agents -- Commercial exploitation of pharmaceutical nanosystems -- Future perspectives -- Multiple Choice -- Problem Sets -- Case Studies -- Suggested Reading.
  • Digital
    Ulrich Meyer, Thomas Meyer, Jörg Handschel, Hans Peter Wiesmann, (eds.).
    Springer2009
  • Digital
    William Wu, Helen H. Zhang, Michael J. Welsh, and Peter B. Kaufman.
    CRCnetBASE2011
    "The third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. Other topics include approaches to grant funding and SiRNA technology. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. This edition features new techniques for every chapter, as well as several new chapters"--Provided by publisher.
  • Digital/Print
    edited by David V. Goeddel.
    Digital : ScienceDirect1990
    Print1990
  • Digital
    maintained by HGMIS for the USDOE Human Genome Program.
  • Digital
    Jan Willem van der Laan, Joseph J. DeGeorge, editors.
    Springer2013
    The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) is a unique project that brings together the regulatory authorities of Europe, Japan and the US and experts from the pharmaceutical industry in the three regions to discuss scientific and technical aspects of product registration. In Japan, the members are the Ministry of Health, Labour and Welfare (MHLW), and the Japan Pharmaceutical Manufacturers Association (JPMA). In Europe, the members are the EU (Representatives of the European Commission and the European Medicines Agency [EMA]), and the European Federation of Pharmaceutical Industries and Associations (EFPIA). In the United States, the members are the Food and Drug Administration (FDA), and the Pharmaceutical Research and Manufacturers of America (PhRMA). The International Federation of Pharmaceutical Manufacturers Associations (IFPMA) is the secretariat of the ICH. Additional members include Observers from WHO, European Free Trade Association (EFTA), and Canada. The Observers represent non-ICH countries and regions. This volume considers one of ICH's major categories, Safety, covering topics relating to in vitro and in vivo pre-clinical studies (Carcinogenicity Testing, Genotoxicity Testing, etc.). Since the start of the ICH process, many guidelines have been written, but in most cases there is a lack of awareness of the many issues that were addressed during the development of the consensus guidances. Further, just as it is important to understand what the guidances state, it is also important to understand the thoughts, debates, and intent of the experts involved, which are not included in the guidance documents. Why has the guideline been written as it is written, why are some topics ignored, and why have some initial guidance proposals have been deleted. These and other related questions and answers are the contents of this book, written by experts who were directly involved in writing the ICH guidances that drive drug development today.
  • Digital
    Kimball Nill.
    CRCnetBASE2006
  • Digital
    Kimball Nill.
    CRCnetBASE2002
  • Digital
    edited by Alain Beck.
    Springer Protocols2013
    Engineering of therapeutic and diagnostic o-glycans on recombinant mucin-type immunoglobulin fusion proteins expressed in CHO cel / Linda Lindberg, Jining Liu, and Jan Holgersson -- Engineering a human-like glycosylation to produce therapeutic glycoproteins based on 6-linked sialylation in CHO cells / Nassimal El Maï [and others] -- Glycoengineered pichia-based expression of monoclonal antibodies / Dongxing Zha -- N-glycosylation humanization for production of therapeutic recombinant glycoproteins in Saccharomyces cerevisiae / Christelle Arico, Christine Bonnet, and Christophe Javaud -- Engineering the baculovirus genome to produce galactosylated antibodies in lepidopteran cells / Sylvie Juliant [and others] -- NanoLC chips MS/MS for the characterization of n-glycopeptides generated from trypsin digestion of a monoclonal antibody / Elsa Wagner-Rousset [and others] -- Cetuximab Fab and Fc n-glycan fast characterization using IdeS digestion and liquid chromatography coupled to electrospray ionization mass spectrometry / Marie-Claire Janin-Bussat [and others] -- Therapeutic antibody glycosylation analysis : a contract research organization perspective in the frame of batch release or comparability support / Arnaud Delobel [and others] -- Mass spectrometric analysis of o-linked oligosaccharides from various recombinant expression systems / Diarmuid T. Kenny [and others] -- Assessing Fc glycan heterogeneity of therapeutic recombinant monoclonal antibodies using NP-HPLC / T. Shantha Raju -- Application of capillary electrophoresis in glycoprotein analysis / Richard R. Rustandi, Carrie Anderson, and Melissa Hamm -- Characterization of glycoprotein biopharmaceutical products by caliper LC90 CE-SDS gel technology / Grace Chen, Sha Ha, and Richard R. Rustandi -- Hydrophobic interaction chromatography to analyze glycoproteins / Richard R. Rustandi -- Lectin glycoprofiling of recombinant therapeutic interleukin-7 / Ludovic Landemarre and Eric Duverger -- Analysis of monoclonal antibodies by sedimentation velocity analytical ultracentrifugation / W. Blaine Stine Jr. -- Noncovalent mass spectrometry for the characterization of antibody/antigen complexes / Cédric Atmanene [and others] -- Conformational analysis of recombinant monoclonal antibodies with hydrogen/deuterium exchange mass spectrometry / Damian Houde and John R. Engen -- Epitope mapping of antibodies by mass spectroscopy : a case study / Victor H. Obungu, Valentina Gelfanova, and Lihua Huang -- Evaluation of antibody-dependent cell cytotoxicity using lactate dehydrogenase (LDH) measurement / Matthieu Broussas, Lucile Broyer, and Liliane Goetsch -- Evaluation of complement-dependent cytotoxicity using ATP measurement and C1q/C4b binding / Lucile Broyer, Liliane Goetsch, and Matthieu Broussas -- Capture of the human IgG1 antibodies by protein A for the kinetic study of h-IgG/FcgR interaction using SPR-based biosensor technology / Thierry Champion and Alain Beck -- Mass spectrometry protocol for the absolute quantification of a monoclonal antibody in serum with immunopurification / François Becher [and others].
  • Digital/Print
    edited by Richard R. Burgess, Murray P. Deutscher.
    Digital : ScienceDirect2009
    Print2009
  • Digital
    [edited by] Sandro Carrara, EPFL, Lausanne, Switzerland, Krzysztof Iniewski, CMOS Emerging Technologies Research, Inc.
    Cambridge2015
  • Digital
    editor, Annie Cavalier, Danièle Spehner, and Bruno M. Humbel.
    CRCnetBASE2009
    Vitreous water / Jacques Dubochet -- Slam-freezing, metal-mirror freezing / Danièle Spehner and Ludwig Edelmann -- Plunge-freezing (Holey carbon method) / Sacha De Carlo -- Controlled vitrification / Peter M. Frederik, Felix de Haas, and Marc M.H. Storms -- BAL-TEC HPM 010 high-pressure freezing machine / Andres Kaech -- High-pressure freezing LEICA EMPACT / Dimitri Vanhecke and Daniel Studer -- Frozen-hydrated macromolecules for structural analysis / Corinne Crucifix, Gabor Papai, and Patrick Schultz -- 2-D crystals / Patrick Bron and Janet Vonck -- Cryo-negative staining / Sacha De Carlo -- Vitrification of dynamic microtubules / Isabelle Arnal, Marine Malleter, and Denis Chrétien -- CEMOVIS : cryo-electron microscopy of vitreous sections / Jacques Dubochet ... [et al.] -- Cryo-electron tomography / Andrew Leis, Manuela Gruska, and Wolfgang Baumeister -- Freeze-substitution / Bruno M. Humbel -- Cryo-fixation, freeze-substitution, rehydration and Tokuyasu cryo-sectioning / York-Dieter Stierhof ... [et al.] -- Freeze-drying and embedding of biological material / Ludwig Edelmann -- The shadow of hydrated biological specimens / Daniel Thomas -- Cryo-fracture of self assembled systems in organic solvent / Marc Schmutz and Philippe J. Mésini -- Progressive lowering of temperature for immunolabeling and in situ hybridization / Annie Cavalier and Danièle Spehner -- Cryo-sectioning according to Tokuyasu / Bruno M. Humbel and York-Dieter Stierhof -- Cryo-preparation procedures for elemental imaging by SIMS and EFTEM / Christian Bordat and Jean-luc Guerquin-Kern -- Correlative light and electron microscopy / Heinz Schwarz and Bruno M. Humbel -- SDS-digested freeze-fracture replica labeling (SDS-FRL) / Yugo Fukazawa ... [et al.] -- Immunolabeling of ultra-thin sections with enlarged 1 nm gold or QDots / York-Dieter Stierhof -- 3-D electron tomography of cells and organelles / Willie J.C. Geerts, Bruno M. Humbel and Arie J. Verkleij -- Final considerations / Hellmuth Sitte.
  • Digital
    by Jean-Francois Denault.
    TandFonline2017
    Preface -- Introduction -- The market research basics -- Collecting data -- primary research -- Secondary research -- Analyzing data -- Estimates and models -- The look n' feel -- Final words -- Bibliography -- Index.
  • Digital
    edited by Anil K. Pabby, Syed S.H. Rizvi, Ana Maria Sastre.
    CRCnetBASE2009
  • Print
    by Kewal K. Jain.
    Status: Not Checked OutLane Catalog Record
  • Digital
    edited by Stefan Dubel and Janice M. Reichert.
    Volume 1. Defining the Right Antibody Composition -- Volume 2. Clinical Development of Antibodies -- Volume 3. Approved Therapeutic Antibodies -- Volume 4. Approved Therapeutic Antibodies and in vivo Diagnostics.
  • Digital
    Kazuyuki Akasaka, Hitoshi Matsuki, editors.
    Springer2015
    Part I. Why and how proteins denature under prssure? -- Part II. Volume, compressibility, fluctuation and interaction in proteins -- Part III. Pressure and functional sub-states in proteins -- Part IV. Pressure and protein folding and assembly -- Part V. Pressure effects on biological membranes -- Part VI. Pressure adaptation and tolerance of proteins and living organisms -- Part VII. High pressure food processing and sterilization -- Part VIII. Pressure effects on motility, physiology and health -- Part IX. Methodology.
  • Digital
    edited by Yacine Graba, René Rezsohazy.
    Springer Protocols2014
    Discovery and classification of homeobox genes in animal genomes / Ferdinand Marlétaz, Jordi Paps, Ignacio Maeso, and Peter W. H. Holland -- How to study hox gene expression and function in mammalian oocytes and early embryos / Delphine Paul, Caroline Sauvegarde, René Rezsohazy, and Isabelle Donnay -- Genetic lineage tracing analysis of anterior hox expressing cells / Brigitte Laforest, Nicolas Bertrand, and Stéphane Zaffran -- A genetic strategy to obtain P-Gal4 elements in the Drosophila hox genes / Luis de Navas, David Foronda, Delia del Saz, and Ernesto Sánchez-Herrero -- Hox complex analysis through BAC recombineering / Mark Parrish, Youngwook Ahn, Christof Nolte, Bony De Kumar and Robb Krumlauf -- The genetics of murine Hox loci : TAMERE, STRING, and PANTHERE to engineer chromosome variants / Patrick Tschopp and Denis Duboule -- Topological organization of Drosophila hox genes using DNA fluorescent in situ hybridization / Frédéric Bantignies and Giacomo Cavalli -- Mining the Cis-regulatory elements of hox clusters / Navneet Kaur Matharu and Rakesh K. Mishra -- Functional analysis of hox genes in Zebrafish / Franck Ladam and Charles G. Sagerström -- Transgenesis in non-model organisms : the case of Parhyale / Zacharias Kontarakis and Anastasios Pavlopoulos -- Tissue specific RNA isolation in Drosophila embryos : a strategy to analyze context dependent transcriptome landscapes using FACS / Arnaud Defaye and Laurent Perrin -- Hox transcriptomics in Drosophila embryos / Maria Polychronidou and Ingrid Lohmann -- Measuring hox-DNA binding by electrophoretic mobility / Kelly Churion [and four others] -- Chromatin immunoprecipitation and chromatin immunoprecipitation with massively parallel sequencing on mouse embryonic tissue / Shilu Amin and Nicoletta Bobola -- chIP for hox proteins from Drosophila imaginal discs / Pavan Agrawal and L. S. Shashidhara -- SELEX-seq : a method for characterizing the compete repertoire of binding site preferences for transcription factor complexes / Todd R. Riley [and six others] -- DamID as an approach to studyinhg long-distance chromatin iteractions / Fabienne Cléard, François Karch, and oRbert K. Maeda -- cgCHIP : a cell type- and gene-specific method for chromatin analysis / Marios Agelopoulos, Daniel J. McKay, and Ricahrd S. Mann -- Bimolecular fluorescence complementation (biFC) in live Drosophila embryos / Marilyne Duffraisse, Bruno Hudry, and Samir Merabet -- Rational drug repurposing using sscMap analysis in a HOX-TALE model of leukemia / Laura M. Kettyle, Fabio G. Liberante, and Alexander Thompson.
  • Digital
    edited by Kursad Turksen.
    Springer Protocols2013
    Primary Culture and Live Imaging of Adult Neural Stem Cells and Their Progeny / Felipe Ortega, Benedikt Berninger, and Marcos R. Costa -- Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology / Marie-Therese Armentero, Patrizia Bossolasco, and Lidia Cova -- High Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes / Gábor Földes and Maxime Mioulane -- A High Content Imaging-Based Approach for Classifying Cellular Phenotypes / Joseph J. Kim, Sebastián L. Vega, and Prabhas V. Moghe -- Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture Conditions / Go Nagamatsu and Toshio Suda -- Imaging and Tracking of Bone-Marrow-Derived Immune and Stem Cells / Youbo Zhao [and four others] -- Co-Visualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3-D DNA Methylation Phenotyping of Stem Cells / Jian Tajbakhsh -- Noninvasive Imaging of Myocardial Blood Flow Recovery in Response to Stem Cell Intervention / HuaLei Zhang and Rong Zhou -- Live Imaging of Early Mouse Embryos Using Fluorescently Labeled Transgenic Mice / Takaya Abe, Shinichi Aizawa, and Toshihiko Fujimori -- Live Imaging, Identifying, and Tracking Single Cells in Complex Populations In Vivo and Ex Vivo / Minjung Kang [and four others] -- Quantitative Evaluation of Stem Cell Grafting in the Central Nervous System of Mice by In Vivo Bioluminescence Imaging and Postmortem Multicolor Histological Analysis / Kristien Reekmans [and eight others] -- Micro-CT Technique for Three Dimensional Visualization of Human Stem Cells / Andrea Farini [and four others] -- Noninvasive Multimodal Imaging of Stem Cell Transplants in the Brain Using Bioluminescence Imaging and Magnetic Resonance Imaging / Annette Tennstaedt [and three others] -- Magnetic Resonance Imaging and Tracking of Stem Cells / Hossein Nejadnik, Rostislav Castillo, and Heike E. Daldrup-Link -- Whole Body MRI and Fluorescent Microscopy for Detection of Stem Cells Labeled with Superparamagnetic Iron Oxide (SPIO) Nanoparticles and DiI Following Intramuscular and Systemic Delivery / Boris Odintsov, Ju Lan Chun, and Suzanne E. Berry -- Molecular Imaging and Tracking Stem Cells in Neurosciences / Toma Spiriev, Nora Sandu, and Bernhard Schaller -- Bioluminescence Imaging of Human Embryonic Stem Cells-Derived Endothelial Cells for Treatment of Myocardial Infarction / Weijun Su [and four others].
  • Digital
    edited by Jose M. Guisan.
    Springer Protocols2013
    New opportunities for immobilization of enzymes / Jose M. Guisan -- Immobilization of enzymes : a literature survey / Beatriz Brena, Paula González-Pombo, and Francisco Batista-Viera -- Glutaraldehyde-mediated protein immobilization / Fernando López-Gallego, Jose M. Guisán, and Lorena Betancor -- Immobilization of enzymes on monofunctional and heterofunctional epoxy-activated supports / Cesar Mateo, Valeria Grazu, and Jose M. Guisan -- Stabilization of enzymes by multipoint covalent immobilization on supports activated with glyoxyl groups / Fernando López-Gallego ... [et al.] -- Oriented covalent immobilization of enzymes on heterofunctional-glyoxyl supports / Cesar Mateo ... [et al.] -- Reversible covalent immobilization of enzymes via disulfide bonds / Karen Ovsejevi, Carmen Manta, and Francisco Batista-Viera -- Immobilization of Candida rugosa lipase on superparamagnetic Fe3O4 nanoparticles for biocatalysis in low-water media / Joyeeta Mukherjee, Kusum Solanki, and Munishwar Nath Gupta -- Immobilization of enzymes by bioaffinity layering / Veena Singh, Meryam Sardar, and Munishwar Nath Gupta -- Immobilization of enzymes on magnetic beads through affinity interactions / Audrey Sassolas, Akhtar Hayat, and Jean-Louis Marty -- Tips for the functionalization of nanoparticles with antibodies / Ester Polo ... [et al.] -- Design and characterization of functional nanoparticles for enhanced bio-performance / Pablo del Pino, Scott G. Mitchell, and Beatriz Pelaz -- Immobilization of enzymes on ethynyl-modified electrodes via click chemistry / Akhtar Hayat ... [et al.] -- Modification of carbon nanotube electrodes with 1-pyrenebutanoic acid, succinimidyl ester for enhanced bioelectrocatalysis / Guinevere Strack ... [et al.] -- Enzyme immobilization by entrapment within a gel network / Audrey Sassolas, Akhtar Hayat, and Jean-Louis Marty -- Practical protocols for lipase immobilization via sol-gel techniques / Manfred T. Reetz -- Improving lipase activity by immobilization and post-immobilization strategies / Jose M. Palomo ... [et al.] -- High activity preparations of lipases and proteases for catalysis in low water containing organic solvents and ionic liquids / Ipsita Roy ... [et al.] -- Biomedical applications of immobilized enzymes : an update / Marta Pastor, Amaia Esquisabel, and José Luis Pedraz -- Immobilization of whole cells by chemical vapor deposition of silica / Susan R. Sizemore ... [et al.] -- Encapsulation of cells in alginate gels / Pello Sánchez ... [et al.] -- Microalgal immobilization methods / Ignacio Moreno-Garrido -- Therapeutic applications of encapsulated cells / Argia Acarregui ... [et al.] -- Whole cell entrapment techniques / Jorge A. Trelles and Cintia W. Rivero.
  • Digital/Print
    John D. Lambris, Kristina N. Ekdahl, Daniel Ricklin, Bo Nilsson, editors.
    Digital : Springer2015
    Print2015
    1. Thromboinflammation in therapeutic medicine -- 2. Complement interactions with blood cells, endothelial cells and microvesicles in thrombotic and inflammatory conditions -- 3. Role of complement on broken surfaces after trauma -- 4. Complement involvement in periodontitis: molecular mechanisms and rational therapeutic approaches -- 5. The lectin pathway of complement and biocompatibility -- 6. Foreign body reaction to subcutaneous implants -- 7. Molecular characterization of macrophage-biomaterial interactions -- 8. Heparan sulfate proteoglycan metabolism and the fate of grafted tissues -- 9. Xenotransplantation of cells, tissues, organs and the Greman research foundation Transregio Collaborative Research Centre 127 -- 10. Macroencapsulated pig islets correct induced diabetes in primates up to 6 months -- 11. Regulation of instant blood mediated inflammatory reaction (IBMIR) in pancreatic islet xeno-transplantation: points for therapeutic interventions -- 12. Cell surface engineering for reguation of immune reactions in cell therapy -- 13. Complement interception across humoral incompatibility in solid organ transplantation: a clinical perspective -- Index.
  • Digital
    Paul J. Fairchild, editor.
    Springer2013
    Recent years have witnessed numerous advances in stem cell research that have suggested novel strategies for the treatment of the chronic and regenerative diseases that pose a growing challenge to the provision of healthcare in the 21<sup>st</sup> century. Nevertheless, there is little doubt that related disciplines have failed to keep pace with such developments, of which the immunology of transplant rejection is perhaps the most relevant to the nascent field of regenerative medicine. Indeed, given that after decades of research into the mechanisms of organ allograft rejection, we remain wholly dependent on immune suppression to ensure acceptance of foreign tissues, we might legitimately ask whether there is any reason why tissues differentiated from stem cells might not suffer the same fate. This volume seeks to address this issue by drawing on the principles of alloantigen recognition to assess the validity of early convictions that embryonic stem cell-derived tissues may prove invisible to the immune system of the host and the more recent belief that induced pluripotent stem cells, derived in a fully autologous manner, will circumvent the issue of immunogenicity. Having debunked such assumptions and established the magnitude of the immunological barriers to be overcome, this book then draws on the expertise of internationally-recognised laboratories around the world to provide a critical assessment of the likely success of applying strategies such as stem cell banking, silencing of genes that confer immunogenicity, and the induction of transplantation tolerance to overcoming the immunological barriers in the future. Indeed, it is only through reaching a consensus in the field that enduring solutions to such an intractable problem may ultimately emerge.
  • Digital
    edited by Evgeny Katz.
    Wiley2014
  • Digital
    David Korpas.
    Springer2013
    History and development of pacing -- Basic principles of cardiac pacemaker technology -- Heart anatomy and physiology -- Pharmacological treatment of cardiac rhythm disorders -- Pacing modes -- Indications for implantable system treatment -- Leads -- Pacing systems -- Pacemaker timing -- Implantable cardioverter-defibrillators -- Cardiac resynchronization therapy -- Implantation, explantation, and replacement of devices and leads -- Patient follow-up -- Electromagnetic compatibility and technical requirements.
  • Digital
    Sibel Yildirim.
    Springer2012
  • Digital
    edited by Elena García-Fruitós.
    Springer Protocols2015
    General introduction : recombinant protein production and purification of insoluble proteins / Neus Ferrer-Miralles ... [et al.] -- Overcoming the solubility problem in E. coli : available approaches for recombinant protein production / Agustín Correa and Pablo Oppezzo -- Optimization of culture parameters and novel strategies to improve protein solubility / Ranjana Arya ... [et al.] -- Cleavable self-aggregating tags (cSAT) for protein expression and purification / Zhanglin Lin ... [et al.] -- Beyond the cytoplasm of Escherichia coli : localizing recombinant proteins where you want them / Jason T. Boock ... [et al.] -- Characterization of amyloid-like properties in bacterial intracellular aggregates / Anna Villar-Pique, Susanna Navarro, and Salvador Ventura -- Co-translational stabilization of insoluble proteins in cell- free expression systems / Lei Kai ... [et al.] -- Functional expression of plant membrane proteins in Lactococcus lactis / Sylvain Boutigny .... [et al.] -- High cell-density expression system : yeast cells in a phalanx efficiently produce a certain range of "Difficult-to-Express" secretory recombinant proteins / Yasuaki Kawarasaki, Takeshi Kurose, and Keisuke Ito -- Insect cells-baculovirus system for the production of difficult to express proteins / Judit Osz-Papai ... [et al.] -- Transient expression in HEK 293 cells : an alternative to E. coli for the production of secreted and intracellular mammalian proteins / Joanne E. Nettleship ... [et al.] -- Recombinant glycoprotein production in human cell lines / Kamilla Swiech ... [et al.] -- Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 / Maria Giuliani ... [et al.] -- Screening methodology for purifying proteins with aggregation problems / Mario Lebendiker, Michal Maes, and Assaf Friedler -- Solubilization and refolding of inclusion body proteins / Anupam Singh, Vaibhav Upadhyay, and Amulya K. Panda -- Bacterial inclusion body purification / Joaquin Seras-Franzoso ... [et al.] -- Characterization of intracellular aggresomes by fluorescent microscopy / Lianwu Fu and Elizabeth Sztul -- Dialysis : a characterization method of aggregation tendency / Mireia Pesarrodona, Ugutz Unzueta, and Esther Vázquez -- Applications of mass spectrometry to the study of protein aggregation / Sílvia Bronsoms and Sebastián A. Trejo -- Insoluble protein assemblies characterized by Fourier transform infrared spectroscopy / Antonino Natalello and Silvia M. Doglia -- Insoluble protein characterization by circular dichroism (CD) spectroscopy and nuclear magnetic resonance (NMR) / Shaveta Goyal ... [et al.] -- Methods for characterization of protein aggregates / Witold Tatkiewicz ... [et al.] -- Predicting the solubility of recombinant proteins in Escherichia coli / Roger G. Harrison and Miguel J. Bagajewicz -- Insoluble protein applications : the use of bacterial inclusion bodies as biocatalysts / Eva Hrabárová , Lucia Achbergerová , and Jozef Nahálka.
  • Digital
    edited by Murugan Ramalingam [and others].
    Wiley2012
  • Digital
    Joanna T. Brougher.
    Springer2014
    Introduction to intellectual property -- Obtaining, enforcing and defending patents -- The patent-eligibility of genes -- Medical procedure patents -- Rights and roles of universities and inventors -- Drug development : balancing innovation, access and profits -- Patent battles part I : small-molecule drugs -- Patent battles part II : the Biosimilars Act and biologics -- International patent law, trade law, and access to drugs.
  • Digital
    edited by Marcus Textor, H. Michelle Grandin.
    Wiley2012
    Frontmatter -- Color Plates -- Stimulus-Responsive Polymers as Intelligent Coatings for Biosensors: Architectures, Response Mechanisms, and Applications / Vinalia Tjong, Jianming Zhang, Ashutosh Chilkoti, Stefan Zauscher -- Smart Surfaces for Point-of-Care Diagnostics / Michael A Nash, Allison L Golden, John M Hoffman, James J Lai, Patrick S Stayton -- Design of Intelligent Surface Modifications and Optimal Liquid Handling for Nanoscale Bioanalytical Sensors / Laurent Feuz, Fredrik H̲̲k, Erik Reimhult -- Intelligent Surfaces for Field-Effect Transistor-Based Nanobiosensing / Akira Matsumoto, Yuji Miyahara, Kazunori Kataoka -- Supported Lipid Bilayers: Intelligent Surfaces for Ion Channel Recordings / Andreas Janshoff, Claudia Steinem -- Antimicrobial and Anti-Inflammatory Intelligent Surfaces / Hans J Griesser, Heike Hall, Toby A Jenkins, Stefani S Griesser, Krasimir Vasilev -- Intelligent Polymer Thin Films and Coatings for Drug Delivery / Alexander N Zelikin, Brigitte St̃dler -- Micro- and Nanopatterning of Active Biomolecules and Cells / Daniel Aydin, Vera C Hirschfeld-Warneken, Ilia Louban, Joachim P Spatz -- Responsive Polymer Coatings for Smart Applications in Chromatography, Drug Delivery Systems, and Cell Sheet Engineering / Roǧrio P Pirraco, Masayuki Yamato, Yoshikatsu Akiyama, Kenichi Nagase, Masamichi Nakayama, Alexandra P Marques, Rui L Reis, Teruo Okano -- Index.
  • Digital
    Alexander P. Demchenko.
    Springer2009
  • Digital
    by Stephen E. Harding, Michael P. Tombs and Gary G. Adams, Berit Smestad Paulsen, Kari Tvete Inngjerdingen and Hilde Barsett.
    CRCnetBASE2017
    Chapter 1. Polysaccharides and their potential for biotechnology -- Chapter 2. Structural polysaccharides -- Chapter 3. Pectic polysaccharides and bioactivity -- Chapter 4. Storage polysaccharides : starch and fructans -- Chapter 5. Marine polysaccharides -- Chapter 6. Some bacterial and synthetic polysaccharides.
  • Digital
    Widya Mulyasasmita.
    The repair of damaged tissues through cell and growth factor administration is a promising strategy in regenerative medicine. However, clinical success has been limited due to the lack of effective delivery methods. While deemed minimally invasive, the conventional approach of direct injection in saline compromises the efficacy of the biological payloads. Post-injection cell survival is often dismally low, owing to the combination of shear forces during injection and hostile environments at the injury site. Growth factors delivered by bolus injections face rapid clearance and distribution to off-target sites, leading to suboptimal concentrations at the sites of therapy and potential side effects in normal tissues. These are major therapeutic hurdles because symptomatic relief following cell therapy is often correlated with the number of surviving donor cells. In the case of growth factor therapy, spatiotemporal control over growth factor distribution is critical for normal recovery, as tissue regeneration is a tightly coordinated process. This thesis presents a biomaterials approach to overcome these hurdles by harnessing polymer and protein engineering strategies to design injectable hydrogel carries for optimal cell and drug delivery. A broad survey of protein-engineered biomaterials is first given in Chapter 1 to provide a foundation for the design principles, synthesis, and characterization methods employed in subsequent chapters. Chapter 2 describes a mechanistic investigation into the physical forces imposed on cells during injection, using alginate polysaccharides as model carriers. This study reveals extensional stresses to be the dominant cause of cell death, and that cell viability can be restored by pre-encapsulation in hydrogel carriers that exhibit thixotropy, or the ability to shear-thin and self-heal. Subsequent chapters describe the design and characterization of a thixotropic protein-engineered hydrogel system called MITCH, or Mixing-Induced Two-Component Hydrogels, and their applications as cell and drug delivery vehicles in regenerative medicine. The crosslinking of the MITCH network relies on the reversible binding between complementary peptide domains, enabling gelation and cell encapsulation by simple mixing at physiological conditions. The design rationale and demonstration of thixotropy, cyto-compatibility, and three-dimensional cell encapsulation are discussed in Chapter 3. In Chapter 4, the specificity and stoichiometric precision of the peptide-peptide crosslinking interactions are highlighted as a distinguishing feature of MITCH. Polymer physics considerations are combined with protein science methodologies to enable the predictable tuning of macroscopic-level gel mechanics through molecular-level variations of component concentration and stoichiometric ratio. The remainder of the thesis focuses on the utility of the MITCH material as a delivery carrier for cell and drug regenerative therapy. Cell protection is demonstrated in Chapter 5, where adipose-derived stem cells injected subcutaneously in mice exhibit improved retention when encapsulated and delivered in MITCH, relative to saline and control biomatrices. Moreover, histological analyses of explants show endogenous cell invasion and signs of native extracellular matrix remodeling at day 3. The simple mixing protocol allows the encapsulation and release of peptide drugs and growth factors in their bioactive state. Chapter 6 describes the engineering of an affinity- and avidity-based peptide drug delivery system, developed by using the molecular recognition domains in MITCH. Fusion of angiogenic peptides to MITCH-specific affinity tags enables drug immobilization, sustained release, and prolonged local drug availability. This controlled release strategy induces higher levels of endothelial cell migration and matrix invasion compared to delivery from saline alone. Chapter 7 presents a therapeutic angiogenesis strategy by dual delivery of human induced stem cell-derived endothelial cells (hiPSC-ECs) and vascular endothelial growth factor (VEGF). This chapter also introduces MITCH 2.0, a new class of protein polymer/synthetic polymer hybrid hydrogel system created to improve tunability and ease of synthesis. Similar to the original version, MITCH 2.0 protects cells during injection and delivers drugs with tunable kinetics. In a murine hindlimb ischemia model, hiPSC-ECs co-delivered with VEGF in MITCH show improved post-transplantation viability and restore blood perfusion to the ischemic limb. All in all, by improving the delivery of cells and biochemical factors, the biomaterials work completed here provides enabling tools to advance other biological research endeavors, to ultimately realize the clinical translation and success of regenerative medicine.
  • Digital
    Fabio Catani, Stefano Zaffagnini, editors.
    Springer2013
    This book discusses the full range of current applications of computer-assisted surgery and robotics in the field of knee surgery, and also considers potential future applications. The impact of computer-assisted surgery on a wide range of surgical procedures is clearly explained. Procedures considered include total knee arthroplasty, unicompartmental knee arthroplasty, cruciate ligament reconstruction, patellofemoral arthroplasty, and revision surgery. In each case, technical aspects are thoroughly addressed in a readily understandable manner. Knee Surgery Using Computer-Assisted Surgery and Robotics will be an ideal guide to this exciting field for both novice and more experienced surgeons who treat knee injuries and disorders.
  • Digital
    edited by Jon Lorsch.
    ScienceDirect2013
    Section I. Cell protocols : basic microbiological techniques. Pouring agar plates and streaking or spreading to isolate individual colonies / Elizabeth Eyler ; Storage of bacteria and yeast / Cheryl M. Koh -- Section II. Cell protocols : cellular fractionation. Cellular fractionation : mammalian cells / Joerg von Hagen and Uwe Michelsen ; Cellular fractionation : yeast cells / Joerg von Hagen and Uwe Michelsen -- Section III. Cell protocols : Knock-outs and gene replacements. Yeast-gene replacement using PCR products / Megan Bergkessel, Christine Guthrie, and John Abelson ; Measuring RNAi knockdown using qPCR / Stuart Milstein, Michael Nguyen, Rachel Meyers, and Antonin de Fougerolles ; Recombineering : using drug cassettes to knock out genes in vivo / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court ; Gene knockouts, in vivo site-directed mutagenesis and other modifications using the delitto perfetto system in Saccharomyces cerevisiae / Samantha Stuckey and Francesca Storici ; Gene targeting and site-specific recombination in mouse ES cells / Konstantinos Anastassiadis, Frank Schnütgen, Harald von Melchner, and A. Francis Stewart ; Recombineering : highly efficient in vivo genetic engineering using single-strand oligos / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court -- Section IV. Cell protocols : microbiological media. Growth media for E. coli / Juliane C. Lessard ; Saccharomyces cerevisiae growth media / Jessica S. Dymond --Section V. Cell protocols : preparation of slides for microscopy. Immunohistochemistry on freely floating fixed tissue sections / Julia Bachman ; Preparation of slides for microscopy from frozen tissue sections / Julia Bachman ; Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry / Kirstie Canene-Adams ; Preparation of cells for microscopy using cytospin / Cheryl M. Koh ; Preparation of cells for microscopy using chamber slides and coverslips / Cheryl M. Koh ; Preparation of cells for microscopy using 'cell blocks' / Cheryl M. Koh -- Section VI. Lipid and carbohydrate protocols. Enzymatic deglycosylation of glycoproteins / Min-Sung Kim and Dan Leahy -- Section VII. Lipid and carbohydrate protocol : making vesicles and micelles. Preparation of fatty acid or phospholipid vesicles by thin-film rehydration / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Preparation of fatty acid micelles / Ting F. Zhu, Itay Budin, and Jack W. Szostak -- Section VIII. Other protocols. Reverse-phase HPLC analysis and purification of small molecules / Kirstie Canene-Adams ; Thin layer chromatography / Marina Santiago and Scott Strobel ; ATP and GTP hydrolysis assays (TLC) / Vaishnavi Rajagopal and Jon R. Lorsch.
  • Digital
    edited by Jon Lorsch.
    ScienceDirect2013
    Explanatory chapter: PCR primer design / Rubén Álvarez-Fernández -- How plasmid preparation kits work / Laura Koontz -- Introducing exogenous DNA into cells / Laura Koontz -- Agarose gel electrophoresis / Laura Koontz -- Analysis of DNA by southern blotting / Gary Glenn and Lefkothea-Vasiliki Andreou -- Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE) / Sara Lopez-Gomollon and Francisco Esteban Nicolas -- Molecular cloning / Juliane C. Lessard -- Rapid creation of stable mammalian cell lines for regulated expression of proteins using the Gateway® recombination cloning technology and Flp-In T-REx® lines / Jessica Spitzer, Markus Landthaler, and Thomas Tuschl -- Restrictionless cloning / Mikkel A. Algire -- Isolation of plasmid DNA from bacteria / Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from bacteria / Lefkothea-Vasiliki Andreou -- Preparation of genomic DNA from Saccharomyces cerevisiae / Jessica S. Dymond -- Isolation of genomic DNA from mammalian cells / Cheryl M. Koh -- Sanger dideoxy sequencing of DNA / Sarah E. Walker and Jon Lorsch -- Preparation of fragment libraries for next-generation sequencing on the applied biosystems SOLiD platform / Srinivasan Yegnasubramanian -- Next generation sequencing / Srinivasan Yegnasubramanian -- Generating mammalian stable cell lines by electroporation / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Transient mammalian cell transfection with polyethylenimine (PEI) / Patti A. Longo, Jennifer M. Kavran, Min-Sung Kim, and Daniel J. Leahy -- Site-directed mutagenesis / Julia Bachman -- PCR-based random mutagenesis / Jessica S. Dymond -- Megaprimer method for mutagenesis of DNA / Craig W. Vander Kooi -- Troubleshooting PCR / Kirstie Canene-Adams -- Quantitative PCR / Jessica S. Dymond -- General PCR / Kirstie Canene-Adams -- Colony PCR / Megan Bergkessel and Christine Guthrie -- Chemical transformation of yeast / Megan Bergkessel and Christine Guthrie -- Transformation of E. coli via electroporation / Juliane C. Lessard -- Transformation of chemically competent E. coli / Rachel Green and Elizabeth J. Rogers.
  • Digital
    edited by Jon Lorsch.
    ScienceDirect2013
    Computational prediction of RNA secondary structure -- Reverse-transcription PCR (RT-PCR) -- Northern blotting -- Explanatory chapter: nuclease protection assays -- In vitro transcription from plasmid or PCR-amplified DNA -- Measurement of in vivo synthesis rates -- Method for measuring mRNA decay rate in saccharomyces cerevisiae -- Analysis of polysomes from bacteria -- Polysome profile analysis - yeast -- Polysome analysis of mammalian cells -- Polysome analysis for determining mRNA and ribosome association in saccharomyces cerevisiae -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates -- Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3' ends, and poly(A) tails of RNA -- RNA radiolabeling -- Fluorescently labeling synthetic RNAs -- Analysis of RNA by analytical polyacrylamide gel -- RNA purification by preparative polyacrylamide gel -- Explanatory chapter: nucleic acid concentration determination -- RNA purification-precipitation methods -- Reverse transcriptase dideoxy sequencing of RNA -- RNA structure experimental analysis-chemical modification -- Structural analysis of RNA backbone using in-line probing.
  • Digital
    edited by Graeme I. Murray.
    Springer Protocols2011
    Laser capture microdissection : methods and applications / Kristen DeCarlo . [and others] -- Laser microdissection for gene expression profiling / Lori A. Field [and others] -- Gene expression using the PALM system / Jian-Xin Lu and Cheuk-Chun Szeto -- Immunoguided microdissection techniques / Michael A. Tangrea [and others] -- Optimized RNA extraction from non-deparaffinized laser-microdissected material / Danny Jonigk [and others] -- Laser capture microdissection for analysis of gene expression in formalin-fixed paraffin-embedded Tissue / Ru Jiang, Rona S. Scott, and Lindsey M. Hutt-Fletcher -- MicroRNA profiling using RNA from microdissected immunostained tissue / Clemens L. Bockmeyer [and others] -- Profiling solid tumor heterogeneity by LCM and biological MS of fresh-frozen tissue sections / Donald J. Johann [and others] -- Amplification testing in breast cancer by multiplex ligation-dependent probe amplification of microdissected tissue / Cathy B. Moelans, Roel A. de Weger, and Paul J. van Diest -- Detection and quantification of MicroRNAs in laser-microdissected formalin-fixed paraffin-embedded breast cancer tissues / Sarkawt M. Khoshnaw [and others] -- Laser capture microdissection applications in breast cancer proteomics / Rene B.H. Braakman [and others] -- Proteomic analysis of laser microdissected ovarian cancer tissue with SELDI-TOF MS / Isabelle Cadron [and others] -- LCM Assisted biomarker discovery from archival neoplastic gastrointestinal tissues / Patricia A. Meitner and Murray B. Resnick -- Purification of diseased cells from Barrett's esophagus and related lesions by laser capture microdissection / Masood A. Shammas and Manjula Y. Rao -- Laser microdissection of intestinal epithelial cells and downstream analysis / Benjamin Funke -- Application of laser microdissection and quantitative PCR to assess the response of esophageal cancer to neoadjuvant chemo-radiotherapy / Claus Hann von Weyhern and Bjorn L.D.M. Brucher -- Oligonucleotide microarray expression profiling of contrasting invasive phenotypes in colorectal cancer / Christopher C. Thorn, Deborah Williams, and Thomas C. Freeman -- Evaluation of gastrointestinal mtDNA depletion in mitochondrial neurogastrointestinal encephalomyopathy (MNGIE) / Carla Giordano and Giulia d'Amati -- Laser microdissection for gene expression study of hepatocellular carcinomas arising in cirrhotic and non-cirrhotic livers / Maria Tretiakova and John Hart -- Laser capture microdissection of pancreatic ductal adeno-carcinoma cells to analyze EzH2 by western blot analysis / Aamer M. Qazi [and others] -- Laser-capture microdissection of renal tubule cells and linear amplification of RNA for microarray profiling and real-time PCR / Susie-Jane Noppert, Susanne Eder, and Michael Rudnicki -- Subcellular renal proximal tubular mitochondrial toxicity with tenofovir treatment / James J. Kohler and Seyed H. Hosseini -- Application of laser-capture microdissection to study renal carcinogenesis / Kerstin Stemmer and Daniel R. Dietrich -- Laser-capture microdissection and transcriptional profiling in archival FFPE tissue in prostate cancer / Ajay Joseph and Vincent J. Gnanapragasam -- Quantitative analysis of the enzymes associated with 5-Fluorouracil metabolism in prostate cancer biopsies / Tomoaki Tanaka -- Microdissection of gonadal tissues for gene expression analyses / Anne Jørgensen, Marlene Danner Dalgaard, and Si Brask Sonne -- Duplex real-time PCR assay for quantifying mitochondrial DNA deletions in laser microdissected single spiral ganglion cells / Adam Markaryan, Erik G. Nelson, and Raul Hinojosa -- Neuronal type-specific gene expression profiling and laser-capture microdissection / Charmaine Y. Pietersen . [et al.] -- Region-specific in situ hybridization-guided laser-capture microdissection on postmortem human brain tissue coupled with gene expression quantification / Rene Bernard, Sharon Burke, and Ilan A. Kerman -- UV-laser microdissection and mRNA expression analysis of individual neurons from postmortem Parkinson's disease brains / Jan Grundemann, Falk Schlaudraff, and Birgit Liss -- Transcriptome profiling of murine spinal neurulation using laser capture microdissection and high-density oligonucleotide microarrays / Shoufeng Cao, Boon-Huat Bay, and George W. Yip -- Probing the CNS microvascular endothelium by immune-guided laser-capture microdissection coupled to quantitative RT-PCR / Nivetha Murugesan [and others] -- Laser-capture microdissection for factor VIII-expressing endothelial cells in cancer tissues / Tomoatsu Kaneko [and others] -- Laser-capture microdissection and analysis of liver endothelial cells from patients with Budd-Chiari syndrome / Selcuk Sozer and Ronald Hoffman -- Laser-capture microdissection of hyperlipidemic/ApoE-/- mouse aorta atherosclerosis / Michael Beer [and others] -- Gene expression profiling in laser-microdissected bone marrow megakaryocytes / Kais Hussein -- Specific RNA collection from the rat endolymphatic sac by laser-capture microdissection (LCM) : LCM of a very small organ surrounded by bony tissues / Kosuke Akiyama [and others] -- Use of laser capture microdissection on adult human articular cartilage for gene expression analysis / Naoshi Fukui, Yasuko Ikeda, and Nobuho Tanaka -- Laser-capture microdissection of developing barley seeds and cDNA array analysis of selected tissues / Johannes Thiel, Diana Weier, and Winfriede Weschke -- Quantitative RT-PCR gene expression analysis of a laser microdissected placenta : an approach to study preeclampsia / Yuditiya Purwosunu [and others].
  • Digital
    Raphael C. Wong, Harley Y. Tse, editors.
    Springer2009
  • Digital
    Uri Gophna, editor.
    Springer2013
    "Although the phenomenon of lateral gene transfer has been known since the 1940s, it was the genomics era that has really revealed the extent and many facets of this evolutionary/genetic phenomenon. Even in the early 2000s with but a handful of genomes available, it became clear that the nature of microorganisms is full of genetic exchange between lineages that are sometimes far apart. The years following this saw an explosion of genomic data, which shook the "tree of life" and also raised doubts about the most appropriate species concepts for prokaryotes. This book represents the manyfold contributions of LGT to the evolution of micro and, to an extent, macroorganisms by focusing on the areas where it has the largest impact: metabolic innovations and adaptations and speciation." Back cover.
  • Digital/Print
    edited by Byron Rubin, Edward A. Dennis.
    Digital : ScienceDirect1997
    Print1997
  • Digital
    Yuki Nakamura, Yonghua Li-Beisson editors.
    Springer2016
    Lipids: from chemical structures, biosynthesis, and analyses to industrial applications -- Roles of lipids in photosynthesis -- DGDG and glycolipids in plants and algae -- Thylakoid development and galactolipid synthesis cyanobacteria -- Role of lipids in chloroplast biogenesis -- Role of MGDG and non-bilayer lipid phases in the structure and dynamics of chloroplast thylakoid membranes -- Chemical genetics in dissecting membrane glycerolipid functions -- Triacylglycerol accumulation in photosynthetic cells in plants and algae -- Cellular organization of triacylglycerol biosynthesis in microalgae -- High-throughput genetics strategies for identifying new components of lipid metabolism in the green alga Chlamydomonas reinhardtii -- Plant sphingolipid meatbolism and function -- Plant surface lipids and epidermis development -- Role of lipid metabolism in plant pollen exine development -- Long-distance lipid signaling and its role in plant development and stress response -- Acyl-CoA-biinding proteins (ACBPs) in plant development -- The rise and fall of Jasmonate biological activities -- Green leaf volatiles in plant signaling and response -- Omics in Chlamydomonas for biofuel production -- Microalgae as a source for VLC-PUFA production -- Understanding sugar catabolism in unicellular cyanobacteria toward the application in biofuel and biomaterial production.
  • Digital
    Katherine Lin Tsai.
    Lab-on-a-Chip devices are attractive for medical diagnostics due to their ability to perform laboratory tasks on small scales. This manuscript explores magnetic nanoparticle-based technologies for low-power, remote actuation in Lab-on-a-Chip systems. Two specific applications -- microfluidic pumping and cell separation -- are investigated. First, chemistry-independent microfluidic pumping using magnetic nanoparticles suspended within a fluid has been demonstrated. Magnetic circuits to generate magnetic field gradients for actuating superparamagnetic nanoparticles in fluids have been fabricated, and changes in fluid velocities when the magnetic circuits are applied have been measured. Results show that the fluid velocity in a microchannel increases 30 [mu]m/sec when a magnetic field gradient of ~3 T/m is applied, and 10 -- 70 [mu]m/sec when a magnetic field gradient of ~5 T/m is applied. The magnetic, optical, and mechanical properties of a magnetic polymer that is composed of SU-8 polymer embedded with nickel nanoparticles (SU8-Ni) have also been characterized. Results show the SU8-Ni composites exhibit weak ferromagnetic behavior and saturate at magnetic fields around 0.2 T, the transmittance of light through SU8-Ni decreases with increasing Ni concentrations, and SU8-Ni has a Young's modulus that is 30 times lower and a hardness that is 1400 times lower than that of bulk Ni. A torsional microactuator made of SU8-Ni has been fabricated to demonstrate its use for magnetic actuation, and a model of the SU8-Ni based on deflection experiments of the actuator has been developed. Micropillars made of SU8-Ni have been fabricated for capturing and concentrating breast cancer cells in microchannels. Magnetic field gradients up to 10,000 T/m have been predicted for an SU8-Ni pillar that is 100 [mu]m tall, 100 [mu]m in diameter, and composed of 12.5% Ni by weight. Experimental results show the SU8-Ni micropillars capture magnetic bead-bound cells when an external magnet is applied and release the cells when the magnet is removed.
  • Digital
    Dokyoon Kim.
    This dissertation presents the basic principles of the magnetic protein chip immunoassay and applications of the magnetic protein chip for the detection of protein biomarkers. The magnetic protein chip is based on the giant magnetoresistive (GMR) spin-valve sensors, and electrical resistance changes of the sensors are measured as signals. Immunoassays were developed using the magnetic protein chips to quantitate protein biomarkers with high sensitivity. The magnetic protein chip immunoassay has several advantages over conventional non-magnetic techniques, such as multiplex capability, smaller sample volume requirement, enhanced signal-to-noise ratio, and facile integration with electronics. Ionizing radiation is high energy radiation that can remove electrons from atoms, and it causes various cellular damages some of which are lethal. Using an in vivo mouse radiation model, we developed protocols for measuring fms-related tyrosine kinase 3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at >= 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A discriminant analysis using both proteins could show improved dose classification accuracy. Our magnetic protein chip immunoassay demonstrated the dose and time responses and low-dose sensitivity that have important advantages in radiation triage biodosimetry. Necrotizing enterocolitis (NEC) is an inflammatory bowel disease often observed in pre-term babies and has high mortality rate. However, timely diagnosis of NEC has been hampered due to its unspecific symptoms and ineffective clinical tests currently available. We developed a magnetic protein chip immunoassay for the validation of NEC biomarkers. Three biomarkers, C-reactive protein (CRP), matrix metalloproteinase-7 (MMP7), and epithelial cell adhesion molecule (EpCAM) were quantitated using a small amount of blood samples. Receiver operating characteristic (ROC) curve analysis combined with bootstrapping technique showed excellent discrimination of NEC from healthy control and NEC from sepsis. Given the generality of the detection scheme used in the magnetic protein chip immunoassay, the magnetic protein chips are expected to hold great potential for medical diagnosis and clinical research.
  • Digital
    [edited by] Jan de Boer, Clemens A. van Blitterswijk.
    Cambridge2013
    "This complete, yet concise, guide introduces you to the rapidly developing field of high throughput screening of biomaterials: materiomics. Bringing together the key concepts and methodologies used to determine biomaterial properties, you will understand the adaptation and application of materomics in areas such as rapid prototyping, lithography and combinatorial chemistry. Each chapter is written by internationally renowned experts, and includes tutorial paragraphs on topics such as biomaterial-banking, imaging, assay development, translational aspects, and informatics. Case studies of state-of-the-art experiments provide illustrative examples, whilst lists of key publications allow you to easily read up on the most relevant background material. Whether you are a professional scientist in industry, a student, or a researcher, this book is not to be missed if you are interested in the latest developments in biomaterials research"--Provided by publisher.
  • Digital
    Qing-Hua Qin.
    CRCnetBASE2013
    Introduction to bone materials -- Basic bone remodeling theory -- Multifield internal bone remodeling -- Multifield surface bone remodeling -- Theoretical models of bone modeling and remodeling -- Effect of parathyroid hormone on bone metabolism -- Cortical bone remodeling under mechanical stimulus -- Bone remodelling under pulsed electromagnetic fields and clinical applications -- Experiments.
  • Digital
    Bert Gordijn, Ruth Chadwick, editors.
    Springer2008
  • Digital
    Peiman Hematti, Armand Keating, editors.
    Springer2013
    1. Mesenchymal stromal cells in regressive medicine: a perspective / Peiman Hematti and Armand Keating -- 2. MSCs: changing hypotheses, paradigms, and controversies on mechanisms of action in repairing tissues / Darwin J. Prockop and Roxanne L. Reger -- 3. MSCs: the need to rethink / Paolo Bianco and Pamela G. Robey -- 4. Characterization of MSCs: from early studies to the present / Mark F. Pittenger -- 5. MSCs as therapeutics / Arnold I. Caplan -- 6. MSC niche for hematopoiesis / Daniel Lucas, Sandra Pinhol and Paul S. Frenette -- 7. Immunomodulatory properties of MSCs / Maria Ester Bernardo, Lynne M. Ball, Franco Locatelli and Willem E. Fibbe -- 8. MSCs and inmate immune responses: a balancing act / Krisztián Németh and Éva Mezey -- 9. MSCs: paracrine effects / Siddiraju V. Boregowda and Donald G. Phinney -- 10. Cross-talk between MSCs and their environments / Thomas P. Lozito and Rocky S. Tuan -- 11. Human MSCs from bone marrow, umbilical cord blood, and adipose tissue: all the same? / Patrick Wuchter and Anthony D. Ho -- 12. MSCs in solid tumors and hematological malignancies: from basic biology to therapeutic applications / Rodrigo Jaeamo, Erika Spaeth, Venkata Battula, Frank Marini and Michael Andreeff -- 13. MSC studies in large-animal models / Bruce A. Bunnell, Christine Gagliardi, and Maria Isabel Ribeiro Dias -- 14. Defining the potential of MSCs with a prenatal large animal model / Grac̦a Almeida-Porada, Christopher D. Orada and Esmail D. Zanjani -- 15. Bench-to-bedside development of MSC therapies: a multidisciplinary approach / Sowmya Viswanathan and Elizabeth J. Read -- 16. cGMP production of MSCs / Derek J. Hei and David H. McKenna, Jr. -- 17. MSCs: the US regulatory perspective / Adrian P. Gee -- 18. MSCs: clinical applications and European regulatory aspects / Jens Reinhardt, Egbert Flory, Isabel Büttel, Christa Schröder, Stefan Fricke, Vladan Vucinic, Michael Cross and Dietger Niederwieser -- 19. Stem cell treatments around the World: boon or bane?/ Alok Srivastava -- 20. Safety issues in MSC therapy / Minoo Battiwalla and A. John Barrett -- 21. In vivo imaging of MSCs / Jeff W.M. Bulte -- 22. National Heart, Lung and Blood Institute support of cellular therapies regenerative medicine / Traci Heath Mondoro and John Thomas -- 23. MSC therapy of inborn errors / Edwin M. Horwitz -- 24. MSCs for enhancement of hematopoietic progenitor cell engraftment and poor graft function / Paolo F. Caimi and Hillard M. Lazarus -- 25. MSCs ro graft-versus-host disease / Jakub Tolar, Katarina Le Blanc and Bruce R. Blazar -- 26. MSCs in pediatric hematopoietic stem cell transplantation / Lynne M. Ball, Maria Ester Bernardo, Franco Locatelli and R. Maarten Egeler -- 27. MSC for ex vivo expansion of umbilical cord blood cells / Ian K. McNiece, Simon N. Robinson, and Elizabeth J. Shpall -- 28. MSCs for autoimmune diseases / Alan Tyndall and Chiara Bocelli -- 29. MSCs for induction of solid organ allograft acceptance / Marc Dahlke, Philipp Renner and Elke Eggenhofer -- 30. MSCc for gastrointestinal disorders / Maria Ester Bernardo, Angela Maria Cometa and Franco Locatelli -- 31. MSCs for cardiac repair / Amish N. Raval and Timothy A. Hacker -- 32. MSCs for treatment of acute lung injury / Vladimir B. Serikov, Anna Krasnodembskaya, Jae Wood Lee, Marlowe W. Eldridge and Michael A. Matthay -- 33. MSCs for diabetes / Luca Inverardi, Giacomo Lanzoni, Juan Dominguez-Bendala and Camillo Ricordi -- 34. MSCs for renal repair / Anna M. Gooch and Christof Westenfelder -- 35. MSCs for the treatment of stroke, spinal cord injury, and traumatic brain injury: from bench work to clinical trials / Bing Yang, Ramy El Khoury, and Sean I. Savitz -- 36. MSCs in reconstructive surgery / Summer E. Hanson and Michael L. Bentz -- 37. MSCs in orthopedic surgery / Summer E. Hanson and Nicholas H. Maassen -- 30. Adipose tissue-derived MSCs: moving to the clinic / Pedro P. Carvalho, Manuela E. Gomes, Rui L. Reis and Jeffrey M. Gimble.
  • Digital
    edited by Christina D. Smolke.
    CRCnetBASEv. 1
    CRCnetBASEv. 2
    [v. 1.] Fundamentals -- [v. 2.] Tools and applications.
  • Digital
    edited by Joakim Dillner.
    Springer Protocols2011
    Genetic research and biobanks / D. Chalmers -- The need to downregulate: A minimal ethical framework for biobank research / M.G. Hansson -- Nordic biological specimen bank cohorts as basis for studies of cancer causes and control: Quality control tools for study cohorts with more than two million sample donors and 130,000 prospective cancers / E. Pukkala -- Biobanks collected for routine healthcare purposes: Build-up and use for epidemiologic research / J. Dillner and K. Andersson -- Biobanks and registers in epidemiologic research on cancer / E. Pukkala -- Study designs for biobank-based epidemiologic research on chronic diseases / E. Laara -- The European prospective investigation into cancer and nutrition biobank / P. Hainaut ... [et al.] -- The aids and cancer specimen resource / L.W. Ayers ... [et al.] -- Specific advantages of twin registries and biobanks / J. Kaprio -- The Swedish multi-generation register / A. Ekbom -- Multigenerational information: The example of the Icelandic genealogy database / H. Tulinius -- Creation of a new prospective research biobank: The example of HUNT3 / K. Hveem -- Best practices for establishing a biobank / G. Hallmans and J.B. Vaught -- Extraction, quantitation, and evaluation of function DNA from various sample types / M. Ivarsson and J. Carlson -- Cervical cytology biobanks as a resource for molecular epidemiology / M. Arbyn ... [et al.] -- Biobanking of fresh frozen tissue from clinical surgical specimens: Transport logistics, sample selection, and histologic characterization / J. Botling and P. Micke -- Protein extraction from solid tissue / C. Ericsson and M. Nister -- Collection and preservation of frozen microorganisms / R. Tedeschi and P. De Paoli -- Handling of solid brain tumor tissue for protein analysis / C. Ericsson and M. Nister -- Blood plasma handling for protein analysis / C. Ericsson and M. Nister -- Biobank informatics: Connecting genotypes and phenotypes / J.E. Litton -- A practical guide to constructing and using tissue microarrays / I. Chandler, R. Houlston and G. Landberg -- Breast cancer genomics based on biobanks / A. Forsti and K. Hemminki -- Monitoring, alarm, and data visualization service on sample preparing and sample storing devices in biobanks / H. Hauksdottir, K. Jonsdottir and A. Thorarinsson -- Fresh frozen tissue: RNA extraction and quality control / J. Botling and P. Micke.
  • Digital
    Walker, John M.
    Springer Protocolsv. 1-24, 1997-2007
  • Digital
    edited by Amy E. Keating.
    ScienceDirect2013
    Computational design of novel protein binders and experimental affinity maturation -- Mining tertiary structural motifs for assessment of designability -- Computational methods for controlling binding specificity -- Flexible backbone sampling methods to model and design protein alternative conformations -- Osprey: protein design with ensembles, flexibility, and provable algorithms -- Scientific benchmarks for guiding macromolecular energy function improvement -- Molecular dynamics simulations for the ranking, evaluation, and refinement of computationally designed proteins -- Multistate protein design using CLEVER and CLASSY -- Using analyses of amino acid coevolution to understand protein structure and function -- Evolution-based design of proteins -- Protein engineering and stabilization from sequence statistics: variation and covariation analysis -- Enzyme engineering by targeted libraries -- Generation of high-performance binding proteins for pepetide motifs by affinity clamping -- Engineering fibronectin-based binding proteins by yeast surface display -- Engineering and analysis of peptide-recognition domain specificities by phage display and deep sequencing -- Efficient sampling of SCHEMA chimera families to identify useful sequence elements -- Protein switch engineering by domain insertion -- Design of chimeric proteins by combination of subdomain-sized fragments -- [alpha]-helix mimicry with [alpha/beta]-peptides.
  • Digital
    Database : NCBI Bookshelf
    This searchable database is a collection of in vivo molecular imaging agents developed for positron emission tomography (PET), single photon emission computed tomography (SPECT), magnetic resonance imaging (MRI), ultrasound (US), computed tomography (CT), and optical imaging. MICAD, a key component of the "Molecular Libraries and Imaging" program of the NIH Roadmap, is being developed by NCBI, the National Center for Biotechnology Information".
  • Digital
    edited by Murugan Ramalingam, Esmaiel Jabbari, Seeram Ramakrishna, and Ali Khadembosseini.
    Wiley2013
    Stem cells and nanotechnology in tissue engineering and regenerative medicine / Rocky Tuan -- Nanofiber technology for controlling stem cell functions and tissue engineering / Seeram Ramakrishna, Shayanti Mukherjee -- Micro- and nanoengineering approaches to developing gradient biomaterials suitable for interface tissue engineering / Ali Khademhosseini, Serge Ostrovidov -- Microengineered polymer and ceramic-based biomaterial scaffolds : a topical review on design, processing, and biocompatibility properties / Bikramjit Basu, Garima Tripathi -- Synthetic enroutes to engineer electrospun scaffolds for stem cells and tissue regeneration / Seeram Ramakrishna, Radhakrishnan Sridhar -- Integrating top-down and bottom-up scaffolding tissue engineering approach for bone regeneration / Esmaiel Jabbari, Yunzhi Yang -- Characterization of the adhesive interactions between cells and biomaterials / John Fisher -- Microfluidic formation of cell-laden hydrogel modules for tissue engineering / Shoji Takeuchi, Yuya Morimoto -- Micro- and nanospheres for tissue engineering / John Jansen -- Micro and nano technologies to engineer bone regeneration / Balaji Sitharaman -- Micro and nanotechnology for vascular tissue engineering / Laura Suggs -- Application of stem cells in ischemic heart disease / Gangapatnam Subrahmanyam, Ravi Shankar.
  • Digital
    Karl Fraser, Zidong Wang, Xiaohui Liu.
    CRCnetBASE2010
  • Digital
    edited by Luis Rueda.
    CRCnetBASE2014
    Microarray Image and Data Analysis: Theory and Practice is a compilation of the latest and greatest microarray image and data analysis methods from the multidisciplinary international research community. Delivering a detailed discussion of the biological aspects and applications of microarrays, the book:Describes the key stages of image processing, gridding, segmentation, compression, quantification, and normalizationFeatures cutting-edge approaches to clustering, biclustering, and the reconstruction of regulatory networksCovers different ty.
  • Digital/Print
    Andreas Bosio, Bernhard Gerstmayer, editors.
    Digital : Springer2008
    Print2008
  • Digital
    Prakash S. Bisen, Mousumi Debnath, Godavarthi B.K.S. Prasad.
    Wiley2012
  • Digital
    edited by José-Luis Barredo.
    Springer Protocols2012
  • Digital
    Angela Ruohao Wu.
    Protein--DNA interactions are responsible for numerous critical cellular events: for example, gene expression and silencing are mediated by transcription factor protein binding and histone protein modifications, and DNA replication and repair rely on site specific protein binding. Chromatin immunoprecipitation (ChIP) is the only molecular assay that directly determines, in a living cell, the binding association between a protein of interest and specific genomic loci. It is an indispensible tool in the biologists' toolbox, but the many limitations of this technique prevent broad adoption of ChIP in biological studies. The typical ChIP assay can take up to one week to complete, and the process is technically tricky, yet tedious. ChIP assay yields are also low, thus requiring on the order of millions to billions of cells as starting material, which makes the assay unfeasible for studies on rare or precious samples. Cancer stem cells (CSCs), for instance, are obtained from primary tumors, and FACS sorting of the dissociated tumor cells rarely yields more than ~100,000 CSCs per tumor. This thesis describes the microfluidics-based strategies for performing ChIP. The first design for a microfluidic ChIP design utilizes the automation and scalability aspects of microfluidics to reduce both total and hands-on assay time, and improve throughput. It can take chromatin prepared using any existing ChIP protocol as input, and generate ChIP-qPCR results in just 1 day. The device is shown to be comparable to existing ChIP protocols, and can detect cellular epigenetic changes induced by external cytokine stimulants. It was used to investigate transcription factor binding dynamics in an aging-related pathway, and was able to link transcription factor binding to local changes in histone modifications. The second design of the microfluidic ChIP platform focuses on addressing the cell number requirements of ChIP. In addition to reducing assay time through automation, this design incorporates microfluidic designs that allow whole fixed cells as input, and enables automated ChIP from as few as 2,000 cells. Finally, using this high-sensitivity ChIP device in conjunction with next-generation sequencing technology, a protocol for determining genome-wide epigenetic landscapes from as few as 2,000 cells is developed, with the ultimate goal of performing protein-DNA association studies on CSCs.
  • Digital
    edited by Gareth Jenkins, Colin D. Mansfield.
    Springer Protocols2013
    Present technology and future trends in point-of-care microfluidic diagnostics / Lawrence Kulinsky, Zahra Noroozi, and Marc Madou -- Teaching microfluidic diagnostics using Jell-O® chips / Cheng Wei T. Yang and Eric T. Lagally -- Fundamentals of microfluidics for high school students with no prior knowledge of fluid mechanics / Vishal Tandon and Walter Peck -- Measuring microchannel electroosmotic mobility and zeta potential by the current monitoring method / Chenren Shao and Don L. DeVoe -- Overview of the microfluidic diagnostics commercial landscape / Lily Kim -- Practical aspects of the preparation and filing of patent applications / Fiona Bessoth -- Introduction to in vitro diagnostic device regulatory requirements / Jonathan Day -- Microfluidic device fabrication by thermoplastic hot-embossing / Shuang Yang and Don L. DeVoe -- Introduction to glass microstructuring techniques / Radoslaw Mazurczyk and Colin D. Mansfield -- Glass microstructure capping and bonding techniques / Radoslaw Mazurczyk, Colin D. Mansfield, and Marcin Lygan -- Rapid prototyping of PDMS devices using SU-8 lithography / Gareth Jenkins -- Microfluidic interface technology based on stereolithography for glass-based lab-on-a-chips / Song-I Han and Ki-Ho Han -- Three-dimensional, paper-based microfluidic devices containing internal timers for running time-based diagnostic assays / Scott T. Phillips and Nicole K. Thom -- Thread based devices for low-cost diagnostics / Meital Reches -- Droplet-based microfluidics / Sanjiv Sharma [and others] -- Droplet-based microfluidics for binding assays and kinetics based on FRET / Monpichar Srisa-Art and Sanjiv Sharma -- Surface treatments for microfluidic biocompatibility / N.J. Shirtcliffe, R. Toon, and P. Roach -- Superhydrophobicity for antifouling microfluidic surfaces / N.J. Shirtcliffe and P. Roach -- Application of microfluidic devices for viral diagnosis in developing countries / Samantha M. Hattersley, John Greenman, and Stephen J. Haswell -- Applications of microfluidics for molecular diagnostics / Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale -- Quantitative heterogeneous immunoassays in protein modified polydimethylsiloxane microfluidic channels for rapid detection of disease biomarkers / Peng Li -- Breast cancer diagnostics using microfluidic multiplexed immunohistochemistry / Minseok S. Kim, Seyong Kwon, and Je-Kyun Park -- Charged-coupled device (CCD) detectors for lab-on-a chip (LOC) optical analysis / Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck -- Multilayer microfluidic poly(ethylene glycol) diacrylate hydrogels / Michael P. Cuchiara and Jennifer L. West -- Purification of DNA/RNA in a microfluidic device / Andy Fan, Samantha Byrnes, and Catherine Klapperich -- Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR / Xuefei Leng and Chaoyong James Yang -- Integrated fluidic circuits (IFCs) for digital PCR / Ramesh Ramakrishnan [and others] -- microFIND® approach to fluorescent in situ hybridization (FISH) / Andrea Zanardi, Emanuele Barborini, and Roberta Carbone -- ELISA lab-on-a-chip (ELISA-LOC) / Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov -- Multiplexed surface plasmon resonance imaging for protein biomarker analysis / Eric Ouellet, Louise Lund, and Eric T. Lagally -- Surface acoustic wave (SAW) biosensors : coupling of sensing layers and measurement / Kerstin Lange, Friederike J. Gruhl, and Michael Rapp -- Microchip UV absorbance detection applied to isoelectric focusing of proteins / Junjie Ou and Carolyn L. Ren.
  • Digital
    edited by David J. Carroll.
    Springer Protocols2009
    Expression of exogenous mRNA in Xenopus laevis embryos for the study of cell cycle regulation / Jill C. Sible and Brian N. Wroble -- Use of luciferase chimaera to monitor PLC[zeta] expression in mouse eggs / Karl Swann ... [et al.] -- Analysis of 14-3-3 family member function in Xenopus embryos by microinjection of antisense morpholino oligos / Jeffrey M.S. Lau and Anthony J. Muslin -- A microinjectable biological system, the Xenopus oocyte, as an approach to understanding signal transduction protein function / Katia Cailliau and Edith Browaeys-Poly -- Combining microinjection and immunoblotting to analyse MAP kinase phosphorylation in single starfish oocytes and eggs / David J. Carroll and Wei Hua -- Analysis of signaling pathways in zebrafish development by microinjection / William H. Kinsey -- Protein inhibition by microinjection and RNA-mediated interference in tissue culture cells : complementary approaches to study protein function / Jane R. Stout, Rania S. Rizk, and Claire E. Walczak -- DNA delivery by microinjection for the generation of recombinant mammalian cell lines / Sebastien Chenuet ... [et al.] -- Bacteriophage [phi]C31 integrase mediated transgenesis in Xenopus laevis for protein expression at endogenous levels / Bryan G. Allen and Daniel L. Weeks -- Germline transformation of Caenorhabditis elegans by injection / Pavan Kadandale, Indrani Chatterjee, and Andrew Singson -- Quantitative microinjection of mouse oocytes and eggs / Douglas Kline -- Microinjection of follicle-enclosed mouse oocytes / Laurinda A. Jaffe ... [et al.] -- Functional studies of regulatory genes in the sea urchin embryo / Vincenzo Cavalieri, Maria Di Bernardo, and Giovanni Spinelli -- Exploring the cytoskeleton during intracytoplasmic sperm injection in humans / Vanesa Y. Rawe and Héctor Chemes -- Somatic cell nuclear transfer in the mouse / Satoshi Kishigami and Teruhiko Wakayama.
  • Digital
    Niall Barron, editor.
    Springer2012
    "MicroRNAs are small, non-coding RNA molecules that interact with target mRNAs to modulate their translation. They are now recognized as widespread and critical regulators of gene expression in the cell. This book is aimed at both industry- and academic-based researchers in the Biopharmaceutical production space who wish to learn more about these fascinating molecules..."--Page [4] of cover.
  • Digital
    Guanghui Ma, Zhiguo Su.
    CRCnetBASE2013
    1. Microspheres for enzyme immobilization / Fei Gao, Yuxia Wang, and Guanghui Ma -- 2. Microspheres for cell culture / Weiqing Zhou, Guanghui Ma, and Zhiguo Su -- 3. Microcapsules for cell transplantation : design, preparation, and application / Guojun Lv, Ying Zhang, Mingqian Tan, Hongguo Xie, and Xiaojun Ma -- 4. Microspheres for the separation and refolding of proteins with an emphasis on particles made of agarose / Jan-Christer Janson -- 5. Microspheres for separation of bioactive small molecules / Fangling Gong, Guifeng Zhang, and Zhiguo Su -- 6. Microspheres for separation of PEG-modified biomolecules / Yongdong Huang, Yanqin Zhai, and Zhiguo Su -- 7. Microspheres for solid-phase organic synthesis / Jing Zhang and Zhiguo Su -- 8. Microspheres for solid-phase modification of proteins / Tao Hu and Zhiguo Su -- 9. Microspheres and microcapsules for protein drug delivery / Lianyan Wang, Tingyuan Yang, and Guanghui Ma -- 10. Micro/nanospheres for gene drug delivery / Jie Wu and Guanghui Ma -- 11. Microspheres for targeting delivery of anticancer drugs / Wei Wei, Zhanguo Yue, Hua Yue, and Guanghui Ma -- 12. Microspheres for targeting delivery to brain / Chen Jiang, Xinguo Jiang, Yang Liu, Kun Shao, and Rongqin Huang -- 13. Nano/microspheres in bioimaging and medical diagnosis / Xiaohui Li and Chunying Chen -- 14. Affinity nanoparticles for detection / Haruma Kawaguchi and Hiroshi Handa.
  • Digital
    Gadryn Christopher Higgs.
    With each heart contraction, blood rushes throughout the expansive circulatory system providing oxygen and other nutrients to the body. Cardiomyocytes are the contractile units responsible for these contractions. In the event of ischemia, the heart reduces its contractile output and loses a significant number of cardiomyocytes. In the ischemic region of the heart, the tissue remodels and the mechanical properties differ significantly from healthy tissue. Due to the limited regeneration capabilities of the heart, recent focus has been on understanding how the reintegration of pluripotent-derived cardiomyocytes can help restore contractile function. These pluripotent-derived cardiomyocytes will need to survive in mechanically active environments and contribute to the contractile output of the heart. However, even before these cardiomyocytes can be used, we need to develop an understanding for how primary immature cardiomyocytes should respond to mechanical forces in vitro. This work explores cardiomyocyte contractile ability in the presence of mechanical forces. For this work, we investigate neonatal murine cardiomyocytes. We begin by developing a protein patterning technique suitable for polyacrylamide. With this technique, we achieve repeatable arrays of neonatal cardiomyocytes and control their shape and size. We found this technique improves sarcomere distribution, making them look more mature and like adult cardiomyocytes. Using micro-tools, we then investigate cardiomyocyte contractility in the presence of mechanical forces. These findings suggest that the Frank-Starling law that governs the heart is also applicable to neonatal cardiomyocytes. Later in this work, we introduce the MEMS tool developed to better understand cardiomyocyte contractile forces. We describe the design goals and fabrication process of the micro-device. In addition, we present the calibration and testing results, and demonstrate that this device provides a reliable and alternative way to calibrate substrates used to study contractile forces. In the closing section of this work, we introduce ideas for future studies building on this work, and the work of others.
  • Digital
    Kunal Kumar Ghosh.
    Fluorescence microscopy has emerged as a workhorse of modern biology and medicine. Fluorescence microscopes are indispensable as tools for basic research in the biological sciences, enable platforms for drug discovery in the biotechnology and pharmaceutical industries, and are increasingly aiding in several clinical diagnostic applications. However, state-of-the-art fluorescence microscopes remain bulky and expensive benchtop instruments with an architecture that impedes usage in certain applications and a cost that precludes adoption in large numbers. For example existing benchtop fluorescence microscopes are not amenable for in vivo imaging in animals, with the mouse being a common animal subject, especially during awake, active behavior. For the field of neuroscience in particular, such an experimental capability permits correlating causal cellular processes with animal behavior -- a longstanding goal. Inspired by this need, we have designed a miniature fluorescence microscope that can be borne by a mouse during active behavior. We have fabricated several such microscopes, each less than 2 g in mass, perhaps heralding a transformation in fluorescence microscopy from today's bulky and expensive benchtop paradigm towards miniature and mass-producible devices. Our fabricated microscopes achieve 2.5 μm spatial resolution imaging fields-of-view up to 800 μm x 600 μm at 36 Hz, suitable for cellular-level imaging at high temporal resolution. To facilitate design of our microscopes, we have adopted a modeling-based microscope design methodology, akin to that used in the design of integrated circuits, and have developed a set of tools to model the microscope as an integrated device from the specimen to final digital image. We have experimentally validated fabricated microscopes for in vivo brain imaging in freely behaving mice, specifically using them for: 1) Imaging cerebellar vasculature and hemodynamics during activity; and 2) Imaging cerebellar Purkinje cell Calcium dynamics, analyzing spiking activity of populations of neurons with single neuron specificity during different motor behaviors. We also show how several of these microscopes imaging in parallel can enable new high-throughput imaging solutions with the potential to achieve broad impact, specifically demonstrating high-throughput image-based assays for: 1) Mutant phenotype screening in genetic model species such as the zebrafish; and 2) Multiple well plate cell analyses. Finally, we use a Bayesian iterative image restoration algorithm to enhance acquired microscope images, and show how spatial resolution can be further scaled into the sub-micron regime by leveraging advances in digital image sensing technology in conjunction with our post-acquisition image restoration algorithm.
  • Digital
    Norma Allewell, Linda O. Narhi, Ivan Rayment (eds.).
    Springer2013
    This volume of the series Biophysics for the Life Sciences focuses on the conceptual framework and major research tools of contemporary molecular biophysics. It is designed to enable non-specialists -both students and professionals in other fields - to understand how these approaches can be used across the biosciences and in medicine, agriculture, biotechnology, pharmaceutical development and other fields. The scope of this volume is appropriate for advanced undergraduate and graduate courses in biophysics and biophysical chemistry. The book begins with an overview of the development of molecular biophysics and a brief survey of structural, physical, and chemical principles. Subsequent chapters written by experts present, with examples, the major experimental methods: optical spectroscopy, X-ray and neutron diffraction and scattering, nuclear magnetic resonance, electron paramagnetic resonance, mass spectrometry, and single molecule methods. The relationship between the biophysical properties of biological macromolecules and their roles as molecular machines is emphasized throughout and illustrated with three examples DNA helicases, rotary motor ATPases, and myosin.The concluding chapter discusses future prospects in X-ray and neutron scattering, mass spectrometry, and pharmaceutical development.
  • Digital
    edited by George P. Patrinos, Wilhelm J. Ansorge.
    ScienceDirect2010
    Chapter 1. Molecular Diagnostics: Past, Present, and Future / George P. Patrinos, Wilhelm J. Ansorge -- Chapter 2 . Allele-Specific Mutation Detection / Aglaia Athanassiadou, Eleana F. Stavrou, Adamandia Papachatzopoulou, George P. Patrinos -- Chapter 3. Enzymatic and Chemical Cleavage Methods to Identify Genetic Variation / Chinh T. Bui, Emmanuelle Nicolas, Georgina Sallmann, Maria Chiotis, Andreana Lambrinakos, Kylee Rees, Ian Trounce, Richard G.H. Cotton, Lauryn Blakesley, Andrew K. Godwin, Anthony T. Yeung -- Chapter 4. Mutation Detection by Single Strand Conformation Polymorphism and Heteroduplex Analysis / Panayiotis G. Menounos, George P. Patrinos -- Chapter 5. Capillary Electrophoresis / Anja Bosserhoff, Claus Hellerbrand -- Chapter 6. Temperature and Denaturing Gradient Gel Electrophoresis / Hartmut Peters, Peter N. Robinson -- Chapter 7. Real-Time Polymerase Chain Reaction / Lut Overbergh, Anna-Paula Giulietti, Dirk Valckx, Chantal Mathieu -- Chapter 8 - Pyrosequencing / Sharon Marsh -- Chapter 9. Application of Padlock and Selector Probes in Molecular Medicine / Mats Nilsson, Chatarina Larsson, Johan Stenberg, Jenny Göransson, Ida Grundberg, Magnus Isaksson, Tim Conze, Sara Henriksson -- Chapter 10. Molecular Cytogenetics in Molecular Diagnostics / Holger Tönnies -- Chapter 11. Analysis of Human Splicing Defects Using Hybrid Minigenes / Franco Pagani, Francisco E. Baralle -- Chapter 12. Detection of Genomic Duplications and Deletions / Graham R. Taylor, Carol A. Delaney -- Chapter 13. Multiplex Ligation-Dependent Probe Amplification (MLPA) and Methylation-Specific (MS)-MLPA: Multiplex Detection of DNA/mRNA Copy Number and Methylation Changes / Eline M. Sepers, Jan P. Schouten -- Chapter 14. Molecular Techniques for DNA Methylation Studies / Jörg Tost, Ivo G. Gut -- Chapter 15. High-Resolution Melting Curve Analysis for Molecular Diagnostics / Jared S. Farrar, Gudrun H. Reed, Carl T. Wittwer -- Chapter 16. DNA Microarrays and Genetic Testing / Lars Dyrskjøt, Karina Dalsgaard-Sørensen, Marie Stampe-Ostenfeld, Karin Birkenkamp-Demtroder, Kasper Thorsen, Claus L. Andersen, Mogens Kruhøffer, Jens L. Jensen, Torben F. Ørntoft -- Chapter 17. Arrayed Primer Extension Microarrays for Molecular Diagnostics / Neeme Tonisson, Eneli Oitmaa, Kaarel Krjutskov, Janne Pullat, Ilona Lind, Merike Leego, Ants Kurg, Andres Metspalu -- Chapter 18. Application of Proteomics to Disease Diagnostics / Samir Hanash, Ji Qiu, Vitor Faca -- Chapter 19. RNA-Based Variant Detection: The Protein Truncation Test / Johan T. den Dunnen -- Chapter 20. Protein Diagnostics by Proximity Ligation: Combining Multiple Recognition and DNA Amplification for Improved Protein Analyses / Karl-Johan Leuchowius, Ola Söderberg, Masood Kamali-Moghaddam, Malin Jarvius, Irene Weibrecht, Katerina Pardali, Ulf Landegren -- Chapter 21. Mass Spectrometry and its Applications to Functional Proteomics / Thomas Köcher, Giulio Superti-Furga -- Chapter 22. Pharmacogenetics and Pharmacogenomics: Impact on Drug Discovery and Clinical Care / Minoli Perera, Federico Innocenti -- Chapter 23. Nutrigenomics: Integrating Genomic Approaches into Nutrition Research / Lynette R. Ferguson, Martin Philpott, Matthew P.G. Barnett -- Chapter 24. Novel Next-Generation DNA Sequencing Techniques for Ultra High-Throughput Applications in Bio-Medicine / Wilhelm J. Ansorge -- Chapter 25. Locus-Specific and National/Ethnic Mutation Databases: Emerging Tools for Molecular Diagnostics / George P. Patrinos -- Chapter 26. Molecular Diagnostic Applications in Forensic Science / Bruce Budowle, John V. Planz, Rowan Campbell, Arthur J. Eisenberg -- Chapter 27. Mass Disaster Victim Identification Assisted by DNA Typing / Daniel Corach -- Chapter 28. Detection of Highly Pathogenic Viral Agents: Implications for Therapeutics, Vaccines and Biodefense / Kylene Kehn-Hall, Sina Bavari -- Chapter 29. Identification of Genetically Modified Organisms / Farid E. Ahmed -- Chapter 30. Molecular Diagnostics and Comparative Genomics in Clinical Microbiology / Alex van Belkum -- Chapter 31. Genetic Monitoring of Laboratory Rodents / Jean-Louis Guénet, Fernando J. Benavides -- Chapter 32. Safety Analysis in Retroviral Gene Therapy: Identifying Virus Integration Sites in Gene-Modified Cells / Stephanie Laufs, Luisa Schubert, Patrick Maier, W. Jens Zeller, Stefan Fruehauf -- Chapter 33. Preimplantation Genetic Diagnosis / Martine de Rycke, Karen Sermon -- Chapter 34. Automated DNA Hybridization and Detection / Bert Gold -- Chapter 35. The Use of Microelectronic-Based Techniques in Molecular Diagnostic Assays / Piotr Grodzinski, Michael Ward, Robin Liu, Kathryn Scott, Saul Surrey, Paolo Fortina -- Chapter 36. Human Gene Patents and Genetic Testing / Timothy Caulfield, Yann Joly -- Chapter 37. Genetic Counseling and Ethics in Molecular Diagnostics / Mary Petrou -- Chapter 38. Genetic Testing and Psychology / Andrea Farkas Patenaude -- Chapter 39. General Considerations Concerning Safety in Biomedical Research Laboratories / William E. Grizzle, Walter C. Bell, Jerry Fredenburgh -- Chapter 40. Quality Management in the Laboratory / David Burnett.
  • Digital
    Mousumi Debnath, Godavarthi B.K.S. Prasad, Prakash S. Bisen.
    Springer2010
  • Digital
    [edited by] Wayne W. Grody ... [et al.].
    ScienceDirect2010
    Advances in genomic and proteomic profiling of disease have transformed the field of molecular diagnostics, thus leading the way for a major revolution in clinical practice. While the range of tests for disease detection and staging is rapidly expanding, many physicians lack the knowledge required to determine which tests to order and how to interpret results. This handbook provides a complete guide to the use and interpretation of molecular testing in the clinical arena. No other available resource offers this emphasis, comprehensive scope, and practical utility in the clinical setting.
  • Digital
    Database : NCBI Bookshelf
    This searchable database is a collection of in vivo molecular imaging agents developed for positron emission tomography (PET), single photon emission computed tomography (SPECT), magnetic resonance imaging (MRI), ultrasound (US), computed tomography (CT), and optical imaging. MICAD, a key component of the "Molecular Libraries and Imaging" program of the NIH Roadmap, is being developed by NCBI, the National Center for Biotechnology Information".
  • Digital
    edited by Shree Ram Singh, Vincenzo Coppola.
    Springer Protocols2014
  • Digital/Print
    Eran Zahavy ... [et al.], editors.
    Digital : Springer2012
    Print2012
    Biomolecule/nanomaterial hybrid systems for nanobiotechnology -- Superresolution optical fluctuation imaging (SOFI) -- Application of nanoparticles for the detection and sorting of pathogenic bacteria by flow-cytometry -- Advancing nanostructured porous si-based optical transducers for label free bacteria detection -- Gold fibers as a platform for biosensing -- Surface-enhanced raman spectroscopy of organic molecules adsorbed on metallic nanoparticles -- Quantum dots and fluorescent protein FRET-based biosensors -- Semiconductor quantum dots as FRET acceptors for multiplexed diagnostics and molecular ruler application -- Assembly and microscopic characterization of DNA origami structures -- DNA nanotechnology -- Role of carbohydrate receptors in the macrophage uptake of dextran-coated iron oxide nanoparticles -- Toxicity of gold nanoparticles on somatic and reproductive cells -- Ultrasound activated nano-encapsulated targeted drug delivery and tumour cell poration -- Ultrasound mediated localized drug delivery -- Sonochemical proteinaceous microspheres for wound healing -- Alendronate liposomes for antitumor therapy: activation of [gamma] [delta] T cells and inhibition of tumor growth.
  • Digital
    edited by Christof M. Niemeyer and Chad A. Mirkin.
    [v. 1]. Concepts, applications, and perspectives -- 1 . Biocompatible inorganic devices / Thomas Sawitowski -- 2. Microfluidics meets nano : lab-on-a-chip devices and their potential for nanobiotechnology / Holger Bartos, Friedrich Götz, and Ralf-Peter Peters -- 3. Microcontact printing of proteins / Emmanuel Delamarche -- 4. Cell-nanostructure interactions / Joachim P. Spatz -- 5. Defined networks of neuronal cells in vitro / Andreas Offenhäusser and Angela K. Vogt -- 6. S-Layers / Uwe B. Sleytr ... [et al.] -- 7. Engineered nanopores / Hagan Bayley ... [et al.] -- 8. Genetic approaches to programmed assembly / Stanley Brown -- 9. Microbial nanoparticle production / Murali Sastry ... [et al.] -- 10. Magnetosomes : nanoscale magnetic iron minerals in bacteria / Richard B. Frankel and Dennis A. Bazylinski -- 11. Bacteriorhodopsin and its potential in technical applications / Norbert Hampp and Dieter Oesterhelt -- 12. Polymer Nanocontainers / Alexandra Graff ... [et al.] -- 13. Biomolecular motors operating in engineered environments / Stefan Diez, Jonne H. Helenius, and Jonathon Howard -- 14. Nanoparticle-biomaterial hybrid systems for bioelectronic devices and circuitry / Eugenii Katz and Itamar Willner -- 15. DNA-protein nanostructures / Christof M. Neimeyer -- 16. DNA-templated electronics / Erez Braun and Uri Sivan -- 17. Biomimetic fabrication of DNA-based metallic nanowires and networks / Michael Mertig and Wolfgang Pompe -- 18. Mineralization in nanostructured biocompartments : biomimetic ferritins for high-density data storage / Eric L. Mayes and Stephen Mann -- 19. DNA-gold nanoparticle conjugates / C. Shad Thaxton and Chad A. Mirkin -- 20. DNA nanostructures for mechanics and computing : nonlinear thinking with life's central molecule / Nadrian C. Seeman -- 21. Nanoparticles as non-viral transfection agents / M.N.V. Ravi Kumar, Udo Bakowsky, and Claus-Michael Lehr -- 22. Luminescent quantum dots for biological labeling / Xiaohu Gao and Shuming Nie -- 23. Nanoparticle molecular labels / James F. Hainfeld, Richard D. Powell, and Gerhard W. Hacker -- 24. Surface biology : analysis of biomolecular structure by atomic force microscopy and molecular pulling / Emin Oroudjev, Signe Danielsen and Helen G. Hansma -- 25. Force spectroscopy / Markus Seitz -- 26. Biofunctionalized nanoparticles for surface-enhanced raman scattering and surface plasmon resonance / Mahnaz El-Kouedi and Christine D. Keating -- 27. Bioconjugated silica nanoparticles for bioanalytical applications / Timothy J. Drake, Xiaojun Julia Zhao, and Weihong Tan. -- v. 2. More concepts and applications -- Part I. Self-Assembly and Nanoparticles: Novel Principles -- ch. 1. Self-Assembled Artificial Transmembrane Ion Channels / Mary S. Gin, Emily G. Schmidt and Pinaki Talukdar, p. 1-15 -- ch. 2. Self-Assembling Nanostructures from Coiled-Coil Peptides / Maxim G. Ryadnov and Derek N. Woolfson, p. 17-38 -- ch. 3. Synthesis and Assembly of Nanoparticles and Nanostructures Using Bio-Derived Templates / Erik Dujardin and Stephen Mann, p. 39-63 -- ch. 4. Proteins and Nanoparticles: Covalent and Noncovalent Conjugates / Rochelle R. Arvizo, Mrinmoy De and Vincent M. Rotello, p. 65-78 -- ch. 5. Self-Assembling DNA Nanostructures for Patterned Molecular Assembly / Thomas H. LaBean, Kurt V. Gothelf and John H. Reif, p. 79-97 -- ch. 6. Biocatalytic Growth of Nanoparticles for Sensors and Circuitry / Ronan Baron, Bilha Willner and Itamar Willner, p. 99-121 -- Part II. Nanostructures for Analytics -- ch. 7. Nanoparticles for Electrochemical Bioassays / Joseph Wang, p. 123-140 -- ch. 8. Luminescent Semiconductor Quantum Dots in Biology / Thomas Pons, Aaron R. Clapp, Igor L. Medintz and Hedi Mattoussi, p. 141-157 -- ch. 9. Nanoscale Localized Surface Plasmon Resonance Biosensors / Katherine A. Willets, W. Paige Hall, Leif J. Sherry, Xiaoyu Zhang, Jing Zhao and Richard P. Van Duyne, p. 159-173 -- ch. 10. Cantilever Array Sensors for Bioanalysis and Diagnostics / Hans Peter Lang, Martin Hegner and Christoph Gerber, p. 175-195 -- ch. 11. Shear-Force-Controlled Scanning Ion Conductance Microscopy / Tilman E. Schäffer, Boris Anczykowski, Matthias Böcker and Harald Fuchs, p. 197-212 -- ch. 12. Label-Free Nanowire and Nanotube Biomolecular Sensors for In-Vitro Diagnosis of Cancer and other Diseases / James R. Heath, p. 213-232 -- ch. 13. Bionanoarrays / Rafael A. Vega, Khalid Salaita, Joseph J. Kakkassery and Chad A. Mirkin, p. 233-259 -- Part III. Nanostructures for Medicinal Applications --. ch. 14. Biological Barriers to Nanocarrier-Mediated Delivery of Therapeutic and Imaging Agents, p. 261-284 / Rudy Juliano -- ch. 15. Organic Nanoparticles: Adapting Emerging Techniques from the Electronics Industry for the Generation of Shape-Specific, Functionalized Carriers for Applications in Nanomedicine / Larken E. Euliss, Julie A. DuPont and Joseph M. DeSimone, p. 285-303 -- ch. 16. Poly(amidoamine Dendrimer-Based Multifunctional Nanoparticles / Thommey P. Thomas, Rameshwer Shukla, Istvan J. Majoros, Andrzej Myc and James R. Baker, p. 305-319 -- ch. 17. Nanoparticle Contrast Agents for Molecular Magnetic Resonance Imaging / Young-Wook Jun, Jae-Hyun Lee and Jinwoo Cheon, p. 321-346 -- ch. 18. Micro- and Nanoscale Control of Cellular Environment for Tissue Engineering / Ali Khademhosseini, Yibo Ling, Jeffrey M. Karp and Robert Langer, p. 347-364 -- ch. 19. Diagnostic and Therapeutic Targeted Perfluorocarbon Nanoparticles / Patrick M. Winter, Shelton D. Caruthers, Gregory M. Lanza and Samuel A. Wickline, p. 365-380 -- Part IV. Nanomotors -- ch. 20. Biological Nanomotors / Manfred Schliwa, p. 381-399 -- ch. 21. Biologically Inspired Hybrid Nanodevices / David Wendell, Eric Dy, Jordan Patti and Carlo D. Montemagno, p. 401-418.
  • Digital
    edited by Sandra J. Rosenthal and David W. Wright.
    Springer Protocols2013
    High-aspect-ratio gold nanorods : their synthesis and application to image cell-induced strain fields in collagen films / Davin J. Chernak ... [et al.] -- Imaging intracellular quantum dots : fluorescence microscopy and transmission electron microscopy / Craig J. Szymanski ... [et al.] -- Imaging of cell populations in atherosclerosis using quantum dot nanocrystals / Joshua R. Trantum and Ashwath Jayagopal -- Imaging of endothelial progenitor cell subpopulations in angiogenesis using quantum dot nanocrystals / Joshua M. Barnett, John S. Penn, and Ashwath Jayagopal -- Imaging single synaptic vesicles in mammalian central synapses with quantum dots / Qi Zhang -- Quantum dot-based single-molecule microscopy for the study of protein dynamics / Jerry C. Chang and Sandra J. Rosenthal -- Three-dimensional molecular imaging with photothermal optical coherence tomography / Melissa C. Skala ... [et al.] -- Detecting respiratory syncytial virus using nanoparticle-amplified immuno-PCR / Jonas W. Perez ... [et al.] -- Gold nanoparticle-oligonucleotide conjugates for the profiling of malignant melanoma phenotypes / John W. Stone ... [et al.] -- Methods for isolating RNA sequences capable of binding to or mediating the formation of inorganic materials / Carly Jo Carter, Alina Owczarek, and Daniel L. Feldheim -- Single-walled carbon nanotube-mediated small interfering RNA delivery for gastrin-releasing peptide receptor silencing in human neuroblastoma / Jingbo Qiao ... [et al.] -- Amino acid mediated linear assembly of au nanomaterials / Manish Sethi and Marc R. Knecht -- Enzyme-gold nanoparticle bioconjugates : quantification of particle stoichiometry and enzyme specific activity / Jacqueline D. Keighron and Christine D. Keating -- In vivo testing for gold nanoparticle toxicity / Carrie A. Simpson, Brian J. Huffman, and David E. Cliffel -- Methods for studying toxicity of silica-based nanomaterials to Living Cells / Yang Zhao ... [et al.].
  • Digital
    edited by Paolo Bergese, Kimberly Hamad-Schifferli.
    Springer Protocols2013
    Preparation of 2 nm gold nanoparticles for in vitro and in vivo applications / Daniel F. Moyano, Bradley Duncan, and Vincent M. Rotello -- DNA conjugation to nanoparticles / Sunho Park -- Conjugation of nanoparticles to proteins / Marie-Eve Aubin-Tam -- Water-solubilization and functionalization of semiconductor quantum dots / Christina M. Tyrakowski, Adela Isovic, and Preston T. Snee -- Synthesizing and modifying peptides for chemoselective ligation and assembly into quantum dot-peptide bioconjugates / W. Russ Algar ... [et al.] -- Reliable methods for silica coating of au nanoparticles / Isabel Pastoriza-Santos and Luis M. Liz-Marzán -- Surface modifications by polymers for biomolecule conjugation / Laura Sola ... [et al.] -- Functionalization protocols of silicon micro/nano- mechanical biosensors / Francesca Frascella and Carlo Ricciardi -- Stability and aggregation assays of nanoparticles in biological media / James Chen Yong Kah -- Electrochemical measurements of DNA melting on surfaces / Irina Belozerova, Dongbiao Ge, and Rastislav Levicky -- Formation and characterization of the nanoparticle-protein corona / Marco P. Monopoli ... [et al.] -- Electrophoretic implementation of the solution-depletion method for measuring protein adsorption, adsorption kinetics, and adsorption competition among multiple proteins in solution / Hyeran Noh ... [et al.] -- Hyperspectral microscopy for characterization of gold nanoparticles in biological media and cells for toxicity assessment / Christin Grabinski, John Schlager, and Saber Hussain -- Immunocytochemistry, electron tomography, and energy dispersive x-ray spectroscopy (EDXS) on cryosections of human cancer cells doped with stimuli responsive polymeric nanogels loaded with iron oxide nanoparticles / Roberto Marotta ... [et al.] -- Zwitterion siloxane to passivate silica against nonspecific protein adsorption / Zaki G. Estephan and Joseph B. Schlenoff -- Preparation and characterization of DNA block copolymer assemblies loaded with nanoparticles / Xi-Jun Chen, Robert J. Hickey, and So-Jung Park -- Polyaspartic acid coated iron oxide nanoprobes for PET/MRI imaging / Taku Cowger and Jin Xie -- Ligand synthesis and passivation for silver and large gold nanoparticles for single-particle-based sensing and spectroscopy / Daniel Montiel ... [et al.] -- Noncovalent intracellular drug delivery of hydrophobic drugs on au NPs / Tennyson Doane and Clemens Burda -- Modification of carbon nanotubes for gene delivery vectors / Victor Ramos-Perez ... [et al.] -- Lipid-based nanoparticles as nonviral gene delivery vectors / Daniele Pezzoli ... [et al.] -- Stabilizing gold nanoparticle bioconjugates in physiological conditions by PEGylation / Joan Comenge and Víctor F. Puntes.
  • Digital
    edited by Maria E. Gracheva.
    Springer Protocols2012
    Detecting and characterizing individual molecules with single nanopores -- Protein sensing with engineered protein nanopores -- Measurements of DNA immobilized in the alpha-hemolysin nanopore -- DNA unzipping and protein unfolding using nanopores -- DNA characterization with ion beam-sculpted silicon nitride nanopores -- DNA sequencing by nanopore-induced photon emission -- Optical tweezers for mechanical control over DNA in a nanopore -- Analyzing single DNA molecules by nanopore translocation -- DNA characterization by transverse electrical current in a nanochannel -- Optimization of the molecular dynamics method for simulations of DNA and ion transport through biological nanopores -- Polymer translocation through an electrically tunable nanopore in a multilayered semiconductor membrane -- Graphene nanopore devices for DNA sensing -- Measuring single-wall carbon nanotubes with solid-state nanopores -- Passive and electrically actuated solid-state nanopores for sensing and manipulating DNA.
  • Digital
    edited by Ping Wang.
    Springer Protocols2011
    Nanoscale-engineered cytochrome P450 system with a branch structure / Hidehiko Hirakawa and Teruyuki Nagamune -- Chemically induced self-assembly of enzyme nanorings / Brian R. White, Qing Li, and Carston R. Wagner -- Self-assemblies of polymer-enzyme conjugates at oil-water interfaces for interfacial biocatalysis / Guangyu Zhu and Ping Wang -- Molecular assembly-assisted biocatalytic reactions in ionic liquids / Muhammad Moniruzzaman and Masahiro Goto -- Organic-soluble enzyme nano-complexes formed by ion-pairing with surfactants / Songtao Wu, Andreas Buthe, and Ping Wang -- Enzyme-immobilized CNT network probe for in vivo neurotransmitter detection / Gi-Ja Lee [and others] -- Kinesin I ATPase manipulates biohybrids formed from tubulin and carbon nanotubes / Cerasela Zoica Dinu [and others] -- Reversible his-tagged enzyme immobilization on functionalized carbon nanotubes as nanoscale biocatalyst / Liang Wang and Rongrong Jiang -- TiO2 nanoparticle system for sacrificial solar H2 production prepared by rational combination of a hydrogenase with a ruthenium photosensitizer / Erwin Reisner and Fraser A. Armstrong -- Preparation and characterization of single-enzyme nanogels / Jun Ge [and others] -- Fabrication and characterization of bioactive thiol-silicate nanoparticles / Frances Neville and Paul Millneri -- Immobilization of enzymes on fumed silica nanoparticles for applications in nonaqueous media / Juan C. Cruz [and others] -- Microencapsulation of bioactive nanoparticles / Fei Gao, Ping Wang, and Guanghui Ma -- Engineering the logical properties of a genetic and gate / Daniel J. Sayut, Yan Niu, and Lianhong Sun -- Strain engineering strategies for improving whole-cell biocatalysis : engineering Escherichia coli to overproduce xylitol as an example / Jonathan W. Chin and Patrick C. Cirino -- Enzyme-carrying electrospun nanofibers / Hongfei Jia -- Uniform lab-scale biocatalytic nanoporous latex coatings for reactive microorganisms / Jimmy L. Gosse and Michael C. Flickinger -- Entrapment of enzymes in nanoporous sol-gels / Andreas Buthe.
  • Digital
    edited by Manfred Ogris, David Oupicky.
    Springer Protocols2013
    Toxicological aspects for nanomaterial in humans -- Lactate dehydrogenase assay for assessment of polycation cytotoxicity -- Combined fluorimetric caspase 3/7 assay and bradford protein determination for assessment of polycation-mediated cytotoxicity -- Anti-PEG igm production via a PEGylated nano-carrier system for nucleic acid delivery -- Near-infrared optical imaging of nucleic acid nanocarriers in vivo -- Lipids for nucleic acid delivery: Synthesis and particle formation -- Histidine-rich cationic amphipathic peptides for plasmid DNA and siRNA delivery -- Synthesis of polyethylenimine-based nanocarriers for systemic tumor targeting of nucleic acids -- Synthesis of bioreducible polycations with controlled topologies -- Lyophilization of synthetic gene carriers -- Surface- and hydrogel-mediated delivery of nucleic acid nanoparticles -- Layer-by-layer assembled gold nanoparticles for the delivery of nucleic acids -- In situ AFM analysis investigating disassembly of DNA nanoparticles and nano-films -- Enhancing nucleic acid delivery with ultrasound and microbubbles -- Magnetic and acoustically active microbubbles loaded with nucleic acids for gene delivery -- Synthesis of lipidic magnetic nanoparticles for nucleic acid delivery -- Lipopeptide delivery of siRNA to the central nervous system -- Flow cytometry-based cell type-specific assessment of target regulation by pulmonary siRNA delivery -- Liver-targeted gene delivery through retrograde intrabiliary infusion.
  • Digital
    edited by Melba Navarro, Josep A. Planell.
    Springer Protocols2012
    Is nanotechnology the key to unravel and engineer biological processes? -- Synthesis of genetically engineered protein polymers (recombinamers) as an example of advanced self-assembled smart materials -- Design of biomolecules for nanoengineered biomaterials for regenerative medicine -- Stimuli responsive polymers for nanoengineering of biointerfaces -- Micro/nanopatterning of proteins using a nanoimprint-based contact printing technique -- Functionalization of surfaces with synthetic oligonucleotides -- Fabrication of PEG hydrogel microwell arrays for high-throughput single stem cell culture and analysis -- Preparation of polyelectrolyte nanocomplexes containing recombinant human hepatocyte growth factor as potential oral carriers for liver regeneration -- Electrospinning technology in tissue regeneration -- Protein adsorption characterization -- Measuring wettability of biosurfaces at the microscale -- AFM to study bio/nonbio interactions -- Evaluation of cytocompatibility and cell response to boron nitride nanotubes -- Nanobiosensors for in vitro and in vivo analysis of biomolecules -- Novel strategies to engineering biological tissue in vitro -- Enabling biomedical research with designer quantum dots -- The role of nanophotonics in regenerative medicine -- Molecular dynamics methods for modeling complex interactions in biomaterials -- Regenerative nanomedicine: ethical, legal, and social issues.
  • Digital
    Amalya Lumerman Oliver.
    ProQuest Ebook Central2009
    Networks, collaborations, and learning and knowledge-creation -- The biotechnology industry through the lenses of organizational and networks scholarship -- New organizational forms for knowledge-creation in biotechnology -- Scientific entrepreneurship -- Science and discoveries in the context of private and public knowledge-creation and learning -- The search for university-industry collaborations : linear and chaotic networking processes -- Trust in collaborations and the social structure of academic research -- Organizational learning and strategic alliances : recombination and duality of competition and collaboration -- Further directions for understanding interorganizational collaborations and learning.
  • Digital
    Dongjun Shin.
    The increasing demand for physical interaction between humans and robots has led to an interest in robots whose behavior is guaranteed to be safe when they are in close proximity with humans. However, attaining sufficiently high levels of performance while ensuring safety creates formidable challenges in mechanical design, actuation, sensing, and control. To promote safety without compromising performance, a new actuation concept, referred to as hybrid actuation, has been developed. Since low impedance output at high frequencies is essential for robot safety, while optimal passive stiffness is needed for robot performance, the new actuation approach employs a pneumatic artificial muscle as a macro actuator to provide low-frequency torques. Artificial pneumatic muscles provide high force-to-weight ratio and inherent compliance, both of which allow for low impedance actuation. To compensate for the slow and non-linear dynamics of pneumatic actuation, a small electromagnetic actuator collocated at the robot's joint is employed as a mini actuator, which provides high mechanical bandwidth for high performance without increasing the inertia and size of the manipulator. To achieve the appropriate balance between safety and performance, design methodologies were developed that optimally determine key design parameters such as the required mini motor torque capacity, the joint stiffness introduced by an antagonistic pair of muscles, and the pulley radius. Using a testbed, referred to as the Stanford Safety Robot (S2rho), the hybrid actuation was evaluated for position tracking performance, force tracking performance, and impact behavior. The experimental results demonstrate that by significantly improving control performance with the hybrid actuation over performance with pneumatic muscles alone, while reducing the effective inertia significantly, the competing design objectives of safety and performance can be successfully integrated into a single robotic manipulator. As an extension of the hybrid actuation concept, the new design of dual four-degree-of-freedom robotic arms with torso is presented and detailed descriptions of the design are included.
  • Digital
    volume editors, Philippe Poindron, Pascale Piguet.
    Karger2008
    Theoretical considerations on animal models / Poindron, P., Callizot, N., Piguet, P. -- Mouse models with motor neuron defects as a tool for deciphering amyotrophic lateral sclerosis. Applications, limits, and future challenges / Echaniz-Laguna, A. ... [et al.] -- A chronic relapsing animal model for multiple sclerosis. Myelin oligodendrocyte glycoprotein induced experimental autoimmune encephalomyelitis in dark agouti rats / Kinter, J., Zeis, T., Schaeren-Wiemers, N. -- Occlusion of the middle cerebral artery in rats. Refinement of an animal model for human stroke / Cam, E. ... [et al.] -- Pyridoxine-induced peripheral neuropathy. Animal models and uses / Callizot, N., Poindron, P. -- A dopamine-sensitive inherited motor syndrome linked to the frissonnant mutation / Callizot, N., Guenet, J.-L., Poindron, P.
  • Digital
    Avi Ma'ayan, Ben D. MacArthur, editors.
    Springer2012
    Gene Expression Profiling and Regulatory Networks in Single Cells / Fumio Arai, Kentaro Hosokawa, Yoshiko Matsumoto, Hirofumi Toyama and Toshio Suda -- Cell Fate Regulatory Networks / Sonya J. Ridden and Ben D. MacArthur -- Noise in Regulatory Networks / Srinandan Dasmahapatra -- Generalised Modelling in Systems Biology / Thilo Gross -- Relative Quantification Mass Spectrometry Using iTRAQ Isobaric Tags / Richard D. Unwin, Emma Carrick and Anthony D. Whetton -- Deciphering Protein Complexes and Protein Interaction Networks for Stem Cell Pluripotency / Jianlong Wang -- Omics Era in Stem Cell Research: Data Integration of Multi-regulatory Layers / Ana Sevilla, Benjamin Trinite and Ihor R. Lemischka -- High Throughput Affinity Purification and Mass Spectrometry to Determine Protein Complex Interactions / Anna Malovannaya, Rainer B. Lanz, Bert W. O'Malley and Jun Qin -- Network Pharmacology to Aid the Drug Discovery Process / Ruth Dannenfelser, Huilei Xu, Catherine Raimond and Avi Ma'ayan -- What Can Networks Do for You? / Bernhard M. Schuldt, Franz-Josef Müller and Andreas A. Schuppert.
  • Digital
    edited by Jian-Bing Fan.
    Springer Protocols2012
    Controlling miRNA regulation in disease / Willemijn M. Gommans and Eugene Berezikov -- Introduction to miRNA profiling technologies and cross-platform comparison / Sarah Aldridge and James Hadfield -- Stem-loop RT-qPCR for microRNA expression profiling / James Hurley [and others] -- Poly(T) adaptor RT-PCR / Rui-Shi [and others] -- MicroRNA in situ hybridization / Boye Schnack Nielsen -- Agilent microRNA microarray profiling system / Petula N. D'Andrade and Stephanie Fulmer-Smentek -- MiRNA expression profiling using Illumina Universal BeadChips / Jing Chen, Craig S. April, and Jian-Bing Fan -- MicroRNA expression analysis using the Affymetrix platform / Suzanne Dee and robert C. Getts -- Individualized miRNA assay panels using optically encoded beads / Keld Sorensen -- Microfluidic primer extension assay / Markus Beier and Valesca Boisguérin -- MicroRNA profiling using [mu]Paraflo microfluidic array technology / Xiaochuan Zhou [and others] -- MicroRNA expression analysis using the Illumina microRNA-Seq platform / Shujun Luo -- Next-generation sequencing of miRNAs with Roche 454 GS-FLX technology : steps for a successful application / Ana Raquel Soares, Patrícia M. Pereira, and Manuel A.S. Santos -- Methods for small RNA preparation for digital gene expression profiling by next-generation sequencing / Sam E.V. Linsen and Edwin Cuppen -- Profiling of short RNAs using Helicos single-molecule sequencing / Philipp Kapranov, Fatih Ozsolak, and Patrice M. Milos -- DeepBASE : annotation and discovery of microRNAs and other noncoding RNAs from deep-sequencing data / Jian-Hua Yang and Liang-Hu Qu -- PhenomiR : microRNAs in human diseases and biological processes / Andreas Ruepp, Andreas Kowarsch, and Fabian Theis -- MiRNA expression profiling : from reference genes to global mean normalization / Barbara D'haene [and others] -- MiRNA data analysis : next-gen sequencing / Preethi H. Gunaratne [and others] -- Integrated miRNA expression analysis and target prediction / William Ritchie and John E.J. Rasko -- MiRNAs in human cancer / Xiaomin Zhong, George Coukos, and Lin Zhang -- Blood-based miRNA preparation for noninvasive biomarker development / Svenja Debey-Pascher [and others].
  • Digital
    Justin Ainscough, Shinya Yamanaka, Takashi Tada, editors.
    Springer2011
    Annotation Research into the field of stem cell biology has developed exponentially over recent years, and is beginning to offer significant promise for unravelling the molecular basis of a multitude of disease states. Importantly, in addition to offering the opportunity to delve deeply into the mechanisms that drive disease aetiology the research is realistically opening the doors for development of targeted and personalized therapeutic applications that many considered, until recently, to be nothing more that a far fetched dream. This volume provides a timely glimpse into the methods that have been developed to instigate, and the mechanisms that have been identified to drive, the process of nuclear reprogramming, chronicling how the field has developed over the last 50-60 years. Since the early 1950s a small number of notable experiments have provided significant impetus to the field, primarily the demonstration of reprogramming ability, first by the complex cytoplasmic milieu that constitutes the amphibian egg, then that of the mammalian egg, and finally that of the mammalian embryonic stem cell. Most recently, the demonstration that a limited pool of defined molecules is capable of reprogramming a multitude of cell types has provided massive impetus and facilitated transition towards realistic therapeutic application. We have therefore reproduced some of the key articles that elegantly document these dramatic stages of development of the field in an inclusive appendix to the book, for the benefit of readers keen to investigate the history of how the field of stem cell biology has evolved. Owing to the ever broadening nature of this field, and the incredible rate at which it is evolving, the main content of this volume focuses on areas that have shown significant movement in recent years, are most likely to translate into personalized therapeutic application, and thus provide greatest potential for significant impact on human health in the not too distant future. We recognize that research into many other disease states and cell types are all equally worthy of discussion. We would therefore like to acknowledge those researchers involved whose work we have not been able to include in this volume. Nuclear Reprogramming and Stem Cells will serve as a valuable resource for all researchers in the field of stem cell biology, including those just setting out on their career path as well as those already established in the field.
  • Digital
    edited by Debmalya Barh, Kenneth Blum, Margaret Anne Madigan.
    CRCnetBASE2012
    Section 1. Methodology and application -- section 2. Empirical research -- section 3. Computational and systems biology -- section 4. Therapeutics -- section 5. Future perspective.
  • Digital
    Debmalya Barh, Dipali Dhawan, Nirmal Kumar Ganguly, editors.
    Springer2013
    Omics for Personalized Medicine will give to its prospective readers the insight of both the current developments and the future potential of personalized medicine. The book brings into light how the pharmacogenomics and omics technologies are bringing a revolution in transforming the medicine and the health care sector for the better. Students of biomedical research and medicine along with medical professionals will benefit tremendously from the book by gaining from the diverse fields of knowledge of new age personalized medicine presented in the highly detailed chapters of the book. The book chapters are divided into two sections for convenient reading with the first section covering the general aspects of pharmaocogenomic technology that includes latest research and development in omics technologies. The first section also highlights the role of omics in modern clinical trials and even discusses the ethical consideration in pharmocogenomics. The second section is focusing on the development of personalized medicine in several areas of human health. The topics covered range from metabolic and neurological disorders to non-communicable as well as infectious diseases, and even explores the role of pharmacogenomics in cell therapy and transplantation technology. Thirty-four chapters of the book cover several aspects of pharmacogenomics and personalized medicine and have taken into consideration the varied interest of the readers from different fields of biomedical research and medicine. Advent of pharmacogenomics is the future of modern medicine, which has resulted from culmination of decades of research and now is showing the way forward. The book is an honest endeavour of researchers from all over the world to disseminate the latest knowledge and knowhow in personalized medicine to the community health researchers in particular and the educated public in general.
  • Digital
    edited by Santi M. Spampinato.
    Springer Protocols2015
    Overview of genetic analysis of human opioid receptors / Santi M. Spampinato -- Computational structural biology of opioid receptors / Davide Provasi -- Analysis of epigenetic mechanisms regulating opioid receptor gene transcription / Cheol Kyu Hwang [and three others] -- Renilla luciferase reporter assay to study 3'UTR-Driven posttranscriptional regulations of OPRM1 / Gabriele Vincelli and Andrea Bedini -- High-throughput gene expression profiling of opioid-induced alterations in discrete brain areas / Michal Korostynski [and three others] -- Real-time imaging of mu opioid receptors by total internal reflection fluorescence microscopy / Cristina Roman-Vendrell and Guillermo Ariel Yudowski -- In vivo techniques to investigate the internalization profile of opioid receptors / Amynah A. Pradhan [and three others] -- Monitoring opioid receptor dimerization in living cells by bioluminescence resonance energy transfer (BRET) / Monica Baiula -- Analysis of potassium and calcium imaging to assay the function of opioid receptors / Viola Spahn, Dinah Nockemann, and Halina Machelska -- Electrophysiological patch clamp assay to monitor the action of opioid receptors / Viola Spahn, Dinah Nockemann, and Halina Machelska -- Skin-nerve preparation to assay the function of opioid receptors in peripheral endings of sensory neurons / Rabih Moshourab, Yvonne Schmidt, and Halina Machelska -- Mechanical nociception measurement in mice and rats with automated Von Frey equipment / Gabriele Campana and Roberto Rimondini -- Detecting zinc release induced by mu-opioid receptor agonists in brain slices / María Rodríguez-Muñoz [and three others] -- Opioid receptors : methods for detection and their modes of actions in the eye / Shahid Husain -- Evaluation of murine macrophage cytokine production after in vivo morphine treatment / Silvia Franchi [and four others] -- Measurement of macrophage toll-like receptor 4 expression after morphine treatment / Mara Castelli [and three others] -- The role of opioid receptors in migration and wound recovery in vitro in cultured human keratinocytes and fibroblasts / Mei Bigliardi-Qi and Paul L. Bigliardi -- Role of opioid receptors in the reinstatement of opioid- seeking behavior : an overview / Liana Fattore [and three others] -- Analysis of opioid-seeking reinstatement in the rat / Liana Fattore [and three others] -- Induction of a high alcohol consumption in rats and mice : role of opioid receptors / Roberto Rimondini and Gabriele Campana -- Evaluation of social and nonsocial behaviors mediated by opioids in mouse pups / Francesca R. D'Amato.
  • Digital
    edited by Stephen P. Morgan, Felicity R.A.J. Rose, Stephen J. Matcher.
    CRCnetBASE2014
    "This book is focused on the optical techniques that can be applied to regenerative medicine, including optical coherence tomography, acousto-optic imaging, Raman spectroscopy, machine vision, polarized light imaging, fibre optic sensors, second harmonic generation, multi-photon microscopy, coherent anti-Stokes Raman scattering, and polarized light imaging. It covers applications both in fundamental research and in the regenerative medicine industry for tissue engineering products. Each chapter gives an overview of a particular technique, its advantages and limitations in terms of structural and functional information provided, and examples of applications in regenerative medicine. The book provides a practical guide to the most appropriate techniques for a given application. It also offers a summary of major recent advances, such as use of machine vision for tracking growth of three-dimensional constructs in bioreactors and polarization sensitive optical coherence tomography for monitoring collagen alignment in cartilage growth"--Provided by publisher.
  • Digital
    edited by Joydeep Basu, John W. Ludlow.
    Springer Protocols2013
    1. Isolation and characterization of human adipose-derived stem cells for use in tissue engineering / Benjamin M. Buehrer and Bentley Cheatham -- 2. Isolation of smooth muscle cells from bladder for generation of engineered urologic organs / Darell W. McCoy -- 3. Isolation of urothelial cells from bladder tissue / Namrata Sangha -- 4. Isolation of pulsatile cell bodies from esophageal tissue / John W. Ludlow, Joydeep Basu, Christopher W. Genheimer, Kelly I. Guthrie, and Namrata Sangha -- 5. Cell isolation through whole-liver perfusion and preparation of hepatocytes for cytochrome P450 analysis / Sonya O. Meheux -- 6. Ex vivo culture and separation of functional renal cells / Andrew T. Bruce, Kelly I. Guthrie, and Rusty Kelley -- 7. Isolation and myogenic differentiation of mesenchymal stem cells for urologic tissue engineering / Rongpei Wu, Guihua Liu, Shantaram Bharadwaj and Yuanyuan Zhang -- 8. Xeno-free adaptation and culture of human pluripotent stem cells / Tori Sampsell-Barron -- 9. Reverse engineering life : physical and chemical mimetics for controlled stem cell differentiation into cardiomyocytes / Gary R. Skuse and Kathleen A. Lamkin-Kennard -- 10. Phenotypic analysis of bioactive cells for application in regenerative medicine / Teresa Bora Burnette and Andrew T. Bruce -- 11. Preparation and evaluation of natural scaffold materials for kidney regenerative applications / Monica A. Serban, Toyin A. Knight, and Richard G. Payne -- 12. Rapid production of autologous fibrin hydrogels for cellular encapsulation in organ regeneration / Adelola O. Oseni, Peter E. Butler, and Alexander M. Seifalian -- 13. Electrospinning tubular scaffolds with tissue-like mechanical properties and biomimetic surface features / Scott Rapoport -- 14. Design, fabrication, and preparation of synthetic scaffolds for urologic tissue engineering / Richard G. Payne and Toyin A. Knight -- 15. Characterization of a PGA-based scaffold for use in a tissue-engineered neo-urinary conduit / Toyin A. Knight and Richard G. Payne -- 16. Migration assay to evaluate cellular interactions with biomaterials for tissue engineering/regenerative medicine applications / Kelly I. Guthrie, Namrata Sangha, Christopher W. Genheimer, Joydeep Basu, and John W. Ludlow -- 17. Care of rodent models used for preclinical evaluation of tissue-engineered/regenerative medicine product candidates / Kim L. Mihalko -- 18. Bioreactor design considerations for hollow organs / Jeff Fish, Craig Halberstadt, Darell W. McCoy and Neil Robbins -- 19. Construction of a multicoaxial hollow fiber bioreactor / Randall McClelland, Katherine Tech, and Jeffrey M. Macdonald -- 20. Isolation of human cadaveric pancreatic islets for clinical transplantation / Craig Halberstadt, Deana Williams, and Paul Gores -- 21. Microencapsulation of pancreatic islets for use in a bioartificial pancreas / Emmanuel C. Opara, John P. McQuilling, and Alan C. Farney -- 22. Bioengineered skin substitutes / Pedro Lei, Hui You, and Stelios T. Andreadis -- 23. Formulation of selected renal cells for implantation into a kidney / Craig Halberstadt, Neil Robbins, Darrell W. McCoy, Kelly I. Guthrie, Andrew T. Bruce, Toyin A. Knight and Richard G. Payne -- 24. Human liver bioengineering using a whole liver decellularized bioscaffold / Pedro M. Baptista, Dipen Vyas, Emma Moran, Zhan Wang and Shay Soker -- 25. A "living bioreactor" for the production of tissue-engineered small intestine / Daniel E. Levin, Frederic G. Sala, Erik R. Barthel, Allison L. Speer, Xiaogang Hou, Yasuhiro Torshima and Tracy C. Grikscheit -- 26. Tissue engineering of esophagus and small intestine in rodent injury models / Joydeep Basu, Kim L. Mihalko, Elias A. Rivera, Kelly I. Guthrie, Christopher W. Genheimer, Namrata Sangha and John W. Ludlow -- 27. Scanning electron microscopy evaluation of endothelialized tissue-engineered constructs / Sandra L. Johnson -- 28. Genotypic and phenotypic analysis of in vivo tissue regeneration in an animal model / Christopher W. Genheimer -- 29. Histological evaluation of tissue regeneration using biodegradable scaffold seeded by autologous cells for tubular/hollow organ applications / Elias A. Rivera and Manuel J. Jayo.
  • Digital
    Anwar Sunna, Andrew Care, Peter L. Bergquist, editors.
    Springer2017
    1. Programmable bio-surfaces for biomedical applications -- 2. Solid-binding peptides in biomedicine -- 3. Molecular modelling of peptide-based materials for biomedical applications -- 4. Design principles of peptide based self-assembled nanomaterials -- 5. Bioprinting and biofabrication with peptide and protein biomaterials -- 6. Peptides as bio-inspired molecular electronic materials -- 7. Peptide-based materials for cartilage tissue regeneration -- 8. Peptides and drug delivery -- 9. Peptide lipidation-- a synthetic strategy to afford peptide based therapeutics -- 10. Molecular engineering of Conus peptides as therapeutic leads -- 11. Uptake mechanism of cell-penetrating peptides -- 12. Empowering the potential of cell-penetrating peptides for targeted intracellular delivery via molecular self-assembly -- 13. The current role of cell-penetrating peptides in cancer therapy -- Index.
  • Digital/Print
    edited by Carlos A. Guzman, Giora Z. Feuerstein.
    Digital : Springer2009
    Print2009
    Translational medicine: a paradigm shift in modern drug discovery and development: the role of biomarkers / Mark Day, J. Lynn Rutkowski, and Giora Z. Feuerstein -- Natural products in drug discovery: present status and perspectives / Gabriella Molinari -- Protein pharmaceuticals: discovery and preclinical development / Davinder S. Gill -- The role of nanobiotechnology in drug discovery / Kewal K. Jain -- Conotoxin venom peptide therapeutics / Richard J. Lewis -- Shark novel antigen receptors: the next generation of biologic therapeutics? / Caroline Barelle, Davinder Gill, and Keith Charlton -- Immune interventions of human diseases through toll-like receptors / Cevayir Coban, Ken J. Ishii, and Shizuo Akira -- Genome-based vaccine development: a short cut for the future / Danilo Gomes Moriel ... [et al.] -- The antigenome: from protein subunit vaccines to antibody treatments of bacterial infections? / Carmen Giefing, Eszter Nagy, and Alexander von Gabain -- HSV as a vector in vaccine development and gene therapy / Peggy Marconi ... [et al.] -- Virus-like particles as a vaccine delivery system: myths and facts / Polly Roy and Rob Noad -- Applications of bacterial ghosts in biomedicine / Petra Lubitz, Ulrike Beate Mayr, and Werner Lubitz -- Immune modulators with defined molecular targets: cornerstone to optimize rational vaccine design / Thomas Ebensen and Carlos A. Guzmøn -- Innovative approaches to develop prophylactic and therapeutic vaccines against HIV/AIDS / Aurelio Cafaro ... [et al.] -- New strategies to overcome the drawbacks of currently available flu vaccines / Epifanio Fichera ... [et al.].
  • Digital
    edited by Christine E. Pullar.
    CRCnetBASE2011
    Measuring endogenous electric fields / Richard Nuccitelli -- Investigation systems to study the biological effects of weak physiological electric fields / Francis X. Hart -- Endogenous bioelectric signals as morphogenetic controls of development, regeneration, and neoplasm / Michael Levin -- Stem cell physiological responses to noninvasive electrical stimulation / Igor Titushkin ... [et al.] -- Electrical signals control corneal epithelial cell physiology and wound repair / Colin D. McCaig -- Physiological electric fields can direct keratinocyte migration and promote healing in chronic wounds / Christine E. Pullar -- Electrical control of angiogenesis / Entong Wang ... [et al.] -- Inflammatory cell electrotaxis / Francis Lin and Christine E. Pullar -- Effects of DC electric fields on migration of cells of the musculoskeletal system / Najmuddin J. Gunja, Clark T. Hung, and J. Chlöe Bulinski -- Neuronal growth cone guidance by physiological DC electric fields / Ann M. Rajnicek -- Can applied voltages be used to produce spinal cord regeneration and recovery in humans? / Richard Ben Borgens -- Bioelectricty of cancer : voltage-gated ion channels and direct-current electric fields / Mustafa B. A. Djamgoz.
  • Digital
    edited by David Edwards.
    Springer Protocols2016
    Using GenBank / Eric W. Sayers and Ilene Karsch-Mizrachi -- UniProtKB/Swiss-Prot, the manually annotated section of the UniProt knowledgebase : how to use the entry view / Emmanuel Boutet ... [et al.] -- KEGG bioinformatics resource for plant genomics and metabolomics / Minoru Kanehisa -- Plant pathway databases / Pankaj Jaiswal and Björn Usadel -- Plant ontology : a tool for plant genomics / Laurel Cooper and Pankaj Jaiswal -- Ensembl plants : integrating tools for visualizing, mining, and analyzing plant genomics data / Dan Bolser ... [et al.] -- Gramene : a resource for comparative analysis of plants genomes and pathways / Marcela Karey Tello-Ruiz ... [et al.] -- PGSB/MIPS plant genome information resources and concepts for the analysis of complex grass genomes / Manuel Spannagl ... [et al.] -- MaizeGDB : the maize genetics and genomics database / Lisa Harper ... [et al.] -- WheatGenome.info : a resource for wheat genomics resource / Kaitao Lai -- User guidelines for the brassica database : BRAD / Xiaobo Wang, Feng Cheng, and Xiaowu Wang -- TAG sequence identification of genomic regions using TAGdb / Pradeep Ruperao -- Short read alignment using SOAP2 / Bhavna Hurgobin -- Tablet : visualizing next-generation sequence assemblies and mappings / Iain Milne ... [et al.] -- Analysis of genotyping-by-sequencing (GBS) data / Sateesh Kagale ... [et al.] -- Skim-based genotyping by sequencing using a double haploid population to call SNPs, infer gene conversions, and improve genome assemblies / Philipp Emanuel Bayer -- Finding and characterizing repeats in plant genomes / Jacques Nicolas, Pierre Peterlongo, and Sébastien Tempel -- Analysis of RNA-Seq data using TopHat and cufflinks / Sreya Ghosh and Chon-Kit Kenneth Chan.
  • Digital
    edited by James A. Birchler.
    Springer Protocols2011
    Recent advances in plant transformation -- Engineering the plastid genome of nicotiana sylvestris, a diploid model species for plastid genetics -- Homologous recombination in plants : An antireview -- Chromosome painting for plant biotechnology -- Plant B chromosomes -- Telomere truncation in plants -- Engineered plant minichromosomes -- Method for bxb1-mediated site-specific integration in planta -- Targeted mutagenesis in arabidopsis using zinc-finger nucleases -- Vectors and methods for hairpin RNA and artificial microRNA-mediated gene silencing in plants -- Recombinant protein expression in nicotiana -- Chromosome analysis and sorting using flow cytometry -- Super-stretched pachytene chromosomes for plant molecular cytogenetic mapping -- Cytological dissection of the triticeae chromosomes by the gametocidal system -- Development and use of oat-maize chromosome additions and radiation hybrids -- Enhancer trapping in plants -- Chromatin beacons : Global sampling of chromatin physical properties using chromatin charting lines -- Transposable elements as catalysts for chromosome rearrangements.

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Derived from Current Medical Diagnosis & Treatment, AccessMedicine's Quick Medical Diagnosis & Treatment provides topic reviews with key diagnostic and treatment features for more than 500 diseases.

A repository of medical knowledge from internal medicine, cardiology, genetics, pharmacy, diagnosis and management, basic sciences, patient care, and more.

Continuously expanding, all databases in the repository contain the latest editions of selected medical titles.

MicroMedex: Premier pharmaceutical information source containing multiple databases and drug reference tools. Of particular value is DRUGDEX Evaluations, one of the most comprehensive drug sources available.DynaMed Plus is a clinical information resource used to answer questions quickly at the point-of-care. Easy-to-interpret Levels of Evidence help clinicians rapidly determine the quality of the available evidence.

Biomedical and pharmacological abstracting and indexing database of published literature, by Elsevier. Embase® contains over 32 million records from over 8,500 currently published journals (1947-present) and is noteworthy for its extensive coverage of the international pharmaceutical and alternative/complementary medicine literature.

Scopus is the largest abstract and citation database of peer-reviewed literature: scientific journals, books and conference proceedings.A drug information resource containing: American Hospital Formulary System (AHFS), drug formulary for Lucile Packard Children's Hospital (LPCH) and Stanford Hospital & Clinics (SHC), Lexi-Drugs (adverse reactions, dosage and administration, mechanism of action, storage, use, and administration information), Lexi-Calc, Lexi-ID, Lexi-I.V. Compatibility (King Guide), Lexi-Interact, and Lexi-PALS.Cumulative Index to Nursing and Allied Health Literature (CINAHL) contains coverage of nursing and allied health literature.A knowledge database that provides access to topic reviews based on over 6000 clinically relevant articles. The evidence-based content, updated regularly, provides the latest practice guidelines in 59 medical specialties.Provides critical assessments of systematic reviews compiled from a variety of medical journals.Selects from the biomedical literature original studies and systematic reviews that are immediately clinically relevant and then summarizes these articles in an enhanced abstract with expert commentary.

Multidisciplinary coverage of over 10,000 high-impact journals in the sciences, social sciences, and arts and humanities, as well as international proceedings coverage for over 120,000 conferences.

Includes cited reference searching, citation maps, and an analyze tool.

Features systematic reviews that summarize the effects of interventions and makes a determination whether the intervention is efficacious or not.

Cochrane reviews are created through a strict process of compiling and analyzing data from multiple randomized control trials to ensure comprehensiveness and reliability.

Provides systematic coverage of the psychological literature from the 1800s to the present through articles, book chapters and dissertations.BMJ Clinical Evidence. A clinical information tool built around systematic reviews summarizing the current state of knowledge about prevention and treatment of clinical conditions.PIER (Physicians' Information and Education Resource) is a Web-based decision-support tool designed for rapid point-of-care delivery of up-to-date, evidence-based guidance for primary care physicians.Cochrane Central Register of Controlled Trials (CENTRAL) provides access to 300,000 controlled trials that have been identified the Cochrane Collaboration.Provides drug information targeted for patients.A continually updating drug monograph.The National Guideline Clearinghouse (NGC): A comprehensive database of evidence-based clinical practice guidelines and related documents.MedlinePlus: A repository of health information from the National Library of Medicine. Links are from trusted sites. No advertising, no endorsement of commercial companies or productsLPCH CareNotes via MicroMedex: Patient education handouts customized by LPCH clinical staffMicromedex Lab Advisor: Evidence based laboratory test informationA drug database organized by generic name, trade name and drug class.LPCH / Stanford Hospital Formulary.A goldmine of trusted consumer health information from the world's largest medical library.A trusted source of expert advice for and about kids, providing the information necessary to help patients and parents understand their unique needs.Provides patient handouts from the American Academy of Family Physician.Access to the Stanford Health Library for patients.Lane provides access to over 5,000 eBooks many of which provide helpful background material that will prepare you to better tackle primary literature.

Largest, broadest eBook package; covers all sciences, as well as technology (including software), medicine, and humanities.

In addition to covering Wiley and Springer, MyiLibrary is also the only provider for Oxford and Cambridge University Press titles. No seat restrictions.

A collection of biomedical books that can be searched directly by concept, and linked to terms in PubMed abstracts.

A web-based, decision support system for infectious diseases, epidemiology, microbiology and antimicrobial chemotherapy. The database, updated weekly, currently includes 337 diseases, 224 countries, 1,147 microbial taxa and 306 antibacterial (-fungal, -parasitic, -viral) agents and vaccines.

Over 10,000 notes outline the status of specific infections within each country.

Large number of high quality software and database programming titles from O'Reilly. Other software titles are also available from Sams and Prentice Hall. Limited to 7 concurrent users.Vast collection of software and database programming titles from multiple publishers, including Microsoft Press.Largest provider of engineering-related eBooks; includes titles in computer science and biomedical engineering.Over 4,000 full-text e-books covering scientific and technical information from CRC Press and others. Many handbooks and single volume reference sources.Includes peer-reviewed life science and biomedical research protocols compiled from Methods in Molecular Biology, Methods in Molecular Medicine, Methods in Biotechnology, Methods in Pharmacology and Toxicology, Neuromethods, the Biomethods Handbook, the Proteomics Handbook, and Springer Laboratory Manuals.Contains full text access to selected biomedical and nursing books.

Provides online, full-text access to Springer's journal titles as well as journals from other publishers.

Subjects include: life sciences, chemical sciences, environmental sciences, geosciences, computer science, mathematics, medicine, physics and astronomy, engineering and economics. Also includes eBooks.

Collection of over 8 thousand fulltext titles in engineering, math, and basic and applied biomedical research. Coverage is from 1967 to the present.A library of ebooks on a wide array of topics, digitized and made available online in conjunction with the original publishers.
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