Books by Subject
- Adaptive and neutral evolutionary insights from statistical analyses of population genetic data — Integration of sensory and reward information during perceptual decision-making in lateral intraparietal cortex (LIP) (100)
- Integrative modeling for genome-wide regulation of gene expression — What sustains life? : consilient mechanisms for protein-based machines and materials (100)
- Wistar Institute of Anatomy and Biology : history, organization, purposes, equipment. — Wistar Institute of Anatomy and Biology : history, organization, purposes, equipment. (1)
- 2010Zhengqing Ouyang.High-throughput genomics has been increasingly generating the massive amount of genome-wide data. With proper modeling methodologies, we can expect to archive a more comprehensive understanding of the regulatory mechanisms of biological systems. This work presents integrative approaches for the modeling and analysis of gene regulatory systems. In mammals, gene expression regulation is combinatorial in nature, with diverse roles of regulators on target genes. Microarrays (such as Exon Arrays) and RNA-Seq can be used to quantify the whole spectrum of RNA transcripts. ChIP-Seq is being used for the identification of transcription factor (TF) binding sites and histone modification marks. RNA interference (RNAi), coupled with gene expression profiles, allow perturbations of gene regulatory systems. Our approaches extract useful information from those genome-wide measurements for effectively modeling the logic of gene expression regulation. We present a predictive model for the prediction of gene expression from ChIP-Seq signals, based on quantitative modeling of regulator-gene association strength, principal component analysis, and regression-based model selection. We demonstrate the combinatorial regulation of TFs, and their power for explaining genome-wide gene expression variation. We also illustrate the roles of covalent histone modification marks on predicting gene expression and their regulation by TFs. We present a dynamical model of gene expression profiling, and derive the perturbed behaviors of the ordinary differential equation (ODE) system. Based on that, we present a regularized multivariate regression method for inferring the gene regulatory network of a stable cell type. We model the sparsity and stability of the network by a regularization approach. We applied the approaches to both a simulation data set and the RNAi perturbation data in mouse embryonic stem cells.
- 2006 SpringerHelmut König, Ajit Varma (eds.).
- 2012Ye Emily Wu.Nervous system function requires the structural and functional integrity of chemical synapses, the fundamental units of neural communication. Synapse formation requires the packaging of synaptic vesicle (SV) and active zone (AZ) proteins into vesicular cargoes in the cell soma, their long-distance microtubule-dependent transport down the axon and finally, their clustering and assembly into functional complexes at specific sites. The subcellular localization, size, and number of synapses diverse greatly between different types of neurons and are critical determinants of the identity and strength of neural connections. Compared to our knowledge of extrinsic signals and cell-surface molecules that regulate synapse formation and synaptic specificity, less is known about the intrinsic regulatory mechanisms that regulate presynaptic protein transport and assembly to achieve proper spatial distribution of synapses. We have taken advantage of the powerful genetics in Caenorhabditis elegans to investigate this question. Using in vivo time-lapse fluorescence microscopy in a motor neuron DA9 in C. elegans, we show that SV and AZ proteins exhibit extensive co-trafficking and undergo frequent pauses during axonal transport. At the pause sites, the balance between the capture and dissociation of mobile transport packets determines the extent of presynaptic protein clustering. Through forward genetic screens, we identified a molecular regulatory network that controls the capture and dissociation events to dictate the spatial distribution of presynaptic specializations. First, we identified an Arf-like small G protein, ARL-8, that regulates synapse distribution by inhibiting premature clustering of presynaptic cargoes during axonal transport. arl-8 mutants accumulate presynaptic specializations within the proximal axon of several neuronal classes, with a corresponding failure to assemble presynapses distally. Time-lapse imaging analysis revealed that presynaptic cargoes in arl-8 mutants exhibit a decreased tendency to dissociate from immotile clusters and an increased probability of being captured by immotile clusters. We further performed genetic suppressor screens to isolate molecules that functionally interact with arl-8 in presynaptic patterning. We report that loss-of-function mutations in a JKK-1/JNK-1 MAP kinase pathway and several active zone assembly proteins strongly and partially suppress the abnormal distribution of presynaptic proteins in arl-8 mutants. Time-lapse imaging analysis demonstrated that the JNK kinase pathway and active zone assembly molecules inhibit presynaptic protein dissociation. In addition, we found that the kinesin motor UNC-104/KIF1A controls the capture efficiency. Furthermore, UNC-104/KIF1A functions as an effector of ARL-8 by binding specifically to the GTP-bound form of ARL-8. Together, these findings revealed novel intrinsic mechanisms that control the subcellular localization, size, and number of synapses by coordinating axonal transport with presynaptic assembly.
- Introducing biological rhythms : a primer on the temporal organization of life, with implications for health, society, reproduction and the natural environment2006 SpringerWillard L. Koukkari and Robert B. Sothern.The study of biological rhythms -- General features of rhythms : terminology and characteristics -- Physical and biological time -- Photoperiodism -- Biological oscillators and timers : models and mechanisms -- Tidal and lunar rhythms -- Sexuality and reproduction -- Natural resources and agriculture -- Veterinary medicine -- Society -- Clinical medicine -- Autorhythmometry -- Chronobiometry : analyzing for rhythms.
- 2007 Springeredited by Marcello Barbieri.
- 2008 ScienceDirectedited by Terence D. Allen.Also available: Print – 2008
- 2013Bertrade Clemence Ange Mbom.[Beta]-Catenin is a multifunctional protein with critical roles in cell-cell adhesion, Wnt-signaling and the centrosome cycle. Whereas the roles of [beta]-catenin in adhesion and Wnt-signaling are well understood, how [beta]-catenin regulates the centrosome cycle is not. NIMA-related protein kinase 2 (Nek2), which stimulates centrosome separation, binds to and phosphorylates [beta]-catenin. Using in vitro and cell-based assays, we show that Nek2 phosphorylates the same regulatory sites as Glycogen Synthase Kinase 3[beta] (GSK3[beta]) in the N-terminus of [beta]-catenin (S33/S37/T41) as well as additional sites. Significantly, Nek2, not GSK3[beta], is responsible for phosphorylating [beta]-catenin at centrosomes. Nek2 binding promotes [beta]-catenin stability by inhibiting binding of the E3 ligase [beta]-TrCP to [beta]-catenin, thereby preventing [beta]-catenin ubiquitination and degradation. Thus, [beta]-catenin phosphorylated by Nek2 on S33/S37/T41 accumulates at centrosomes in mitosis. We further show that Plk1 regulates Nek2 activity in stabilizing [beta]-catenin. Taken together, these results identify a novel mechanism for regulating [beta]-catenin stability, and provide new insight into a pathway involving Plk1, Nek2 and [beta]-catenin that regulates the centrosome cycle.
- 2014 CRCnetBASEPrakash Singh Bisen, Emeritus Scientist, Defense Research Development Establishment, Defense Research Development Organization, Ministry of Defense, Government of India, Gwalior, India .Microscopy -- Microtome -- pH and Buffers -- Spectrophotometer -- Centrifugation -- Radiation Biology -- Chromatography -- Separation Technology -- Histochemical Techniques -- Cytogenetics -- Biomolecules -- Enzymology -- Membranes -- Photosynthesis and Respiration -- Microbiology -- Soil Biology -- Virology -- Immunochemical Methods -- Genetic Engineering -- Molecular Biology -- Biosensors -- Enzyme Immobilization -- Plant Tissue Culture -- Animal Cell Science and Technology -- Bioprocess Engineering -- Environmental Biology -- Bioinformatics
- 2007 SpringerM.L. López-Gracia and M.A. Ros.
- 2008 SpringerJesper Hoffmeyer, editor.Introduction: Bateson the precursor / Jesper Hoffmeyer -- Angels fear revisited: Gregory Bateson's cybernetic theory of mind applied to religion-science debates / Mary Catherine Bateson -- From thing to relation. On Bateson's bioanthropology / Jesper Hoffmeyer -- What connects the map to the territory? / Tyrone Cashman -- The pattern which connects pleroma to creatura: the autocell bridge from physics to life / Terrence Deacon and Jeremy Sherman -- Bateson's method: double description. What is it? How does it work? What do we learn? / Julie Hui, Tyrone Cashman, and Terrence Deacon -- Gregory Bateson's relevance to current molecular biology / Luis Emilio Bruni -- Process ecology: creatura at large in an open universe / Robert E. Ulanowicz -- Connections in action - bridging implicit and explicit domains / Teresa S.S. Shilhab and Christian Gerlach -- Bateson: biology with meaning / Brian Goodwin -- Gregory Bateson's "uncovery" of ecological aesthetics / Peter Harries-Jones -- Collapsing the wave function of meaning: the epistemological matrix of talk-in-interaction / Donald Favareau -- Re-enchanting evolution: transcending fundamentalisms through a mythopoetic epistemology / Gregory Mengel -- Bateson and Peirce on the pattern that connects and the sacred / Søren Brier -- Bateson, Peirce and the sign of the sacred / Deborah Eicher-Catt.
- 2006 Springered. by Lisa Gould and Michelle L. Sauther.Lemur origins, history of ecological studies on lemurs, and the ecology of the recently extinct (subfossil) lemurs -- Origin of the Malagasy strepsirhine primates / Ian Tattersall -- Notes on the history of ecological studies of Malagasy lemurs / Alison Jolly and R.W. Sussman -- Ecology and extinction of Madagascar's subfossil lemurs / Laurie R. Godfrey, William L. Jungers, and Gary T. Schwartz -- The evolution of lemur traits, basic and unusual patterns -- Impact of ecology on the teeth of extant lemurs : a review of dental adaptations, function, and life history / Frank P. Cuozzo and Nayuta Yamashita -- Big is beautiful : fat storage and hibernation as a strategy to cope with marked seasonality in the fat-tailed dwarf lemur (Cheirogaleus medius) / Joanna Fietz and K.H. Dausmann -- Polyspecific associations of crowned lemurs and Sanford's lemurs in Madagascar / Benjamin Z. Freed -- Cathemerality in lemurs / Deborah J. Curtis -- Adaptations in the aye-aye : a review / Eleanor J. Sterling and Erin E. McCreless -- Connecting ecology and adaptation : summaries and new studies -- Evolutionary divergence in the brown lemur species complex / Steig E. Johnson -- Ecological diversity and seasonal adaptations of mouse lemurs (Microcebus spp.) / Ute Radespiel -- Social pair-bonding and resource defense in wild red-bellied lemurs (Eulemur rubriventer) / Deborah J. Overdorff and Stacey R. Tecot -- Lemur catta ecology : what we know and what we need to know / Lisa Gould -- Impact of seasonality and reproduction on social structure, ranging patterns, and fission-fusion social organization in red ruffed lemurs / Natalie Vasey -- Ecologically enigmatic lemurs : the sifakas of the eastern forests (Propithecus candidus, P. diadema, P. edwardsi, P. perrieri, P. tattersalli) / Mitchell T. Irwin -- Behavioral and ecological adaptations in two small folivorous lemurs with different social organization : Avahi and Lepilemur / Urs Thalmann -- The feeding ecology and related adaptations of Indri indri / Joyce A. Powzyk and Christopher B. Mowry -- Behavior and ecology of gentle lemurs (genus Hapalemur) / Chia L. Tan -- Lemur adaptations to a changing environment, natural and anthropogenic stress -- Considering climate change effects in lemur ecology and conservation / Patricia C. Wright -- Diet composition, foraging, and feeding behavior in relation to habitat disturbance : implications for the adaptability of ruffed lemurs (Varecia variegata editorium) in Manombo Forest, Madagascar / Jonah Ratsimbazafy -- Overview on the health and disease ecology of wild lemurs : conservation implications / Randall E. Junge and Michelle L. Sauther
- 2006 Springeredited by J. Seckbach.
- 2007 Springerby Ricardo Amils, Cynan Ellis-Evans and Helmut Hinghofer-Szalkay.
- 2008 Springeredited by Eric J. Sargis and Marian Dagosto.Earliest evidence of Deltatheroida (Mammalia: Metatheria) from the early Cretaceous of North America / Brian M. Davis, Richard L. Cifelli and Zofia Kielan-Jaworowska -- Evolution of hind limb proportions in kangaroos (Marsupialia: Macropodoidea) / Benjamin P. Kear ... [et al.] -- Changing views in paleontology : the story of a giant (Megatherium, Xenarthra) / Christine Argot -- Evolutionary morphology of the Tenrecoidea (Mammalia) forelimb skeleton / Justine A. Salton and Eric J. Sargis -- Postcranial morphology of Apheliscus and Haplomylus (Condylarthra, Apheliscidae) : evidence for a Paleocene Holarctic origin of Macroscelidea / Tonya A. Penkrot ... [et al.] -- Postcranial skeleton of the Upper Paleocene (Itaboraian) "Condylarthra" (Mammalia) of the Itaboraí Basin, Brazil / Lilian P. Berqvist -- Postcranial osteology of mammals from Salla, Bolivia (late Oligocene) : form, function, and phylogenetic implications / Bruce J. Shockey and Frederico Anaya -- Evolution of the proximal third phalanx in Oligocene-Miocene equids, and the utility of phalangeal indices in phylogeny reconstruction / Jay A. O'Sullivan -- Adaptive zones and the pinniped ankle : a three-dimensional quantitative analysis of carnivoran tarsal evolution / P. David Polly -- The biogeographic origins of Primates and Euprimates : east, west, north, or south of Eden? / Mary T. Silcox -- Evaluating the mitten-gliding hypothesis for Paromomyidae and Micromomyidae (Mammalia, "Plesiadapiformes") using comparative functional morphology of new Paleogene skeletons / Douglas M. Boyer and Jonathan I. Bloch -- Morphological diversity in the skulls of large adapines (Primates, Adapiformes) and its systematic implications / Marc Godinot and Sébastien Couette -- Primate tibiae from the middle Eocene Shanghuang fissure-fillings of eastern China / Marian Dagosto ... [et al.] -- Rooneyia, postorbital closure, and the beginnings of the age of Anthropoidea / Alfred L. Rosenberger, Russell Hogg and Sai Man Wong -- Epitensoric position of the chorda tympani in Anthropoidea : a new synapomorphic character, with remarks on the fissura Glaseri in Primates / Wolfgang Maier -- Evolutionary morphology of the guenon postcranium and its taxonomic implications / Eric J. Sargis, Carl J. Terranova and Daniel L. Gebo -- Analysis of selected hominoid joint surfaces using laser scanning and geometric morphometrics : a preliminary report / William E.H. Harcourt-Smith ... [et al.] -- Comparative primate bone microstructure: records of life history, function, and phylogeny / Johanna Warshaw.
- [v. 3], 2016edited by Alexander Stone Macnow, MD.The Cell -- Reproduction -- Embryogenesis and Development -- The Nervous System -- The Endocrine System -- The Respiratory System -- The Cardiovascular System -- The Immune System -- The Digestive System -- Homeostasis -- Musculoskeletal System -- Genetics and Evolution.
- 2006 ScienceDirectedited by James Inglese.Also available: Print – 2006
- 2013Brett Theodore Staahl.During the development of the vertebrate nervous system neural progenitors divide, generate progeny that exit mitosis and then migrate to sites where they elaborate specific morphologies and synaptic connections. Mitotic exit in neurons is accompanied by an essential switch in the Mammalian SWI/SNF (also called BAF) ATP-dependent chromatin regulatory complexes from the neural progenitor npBAF to neuron-specific nBAF complexes. We elucidated the mechanism of a microRNA/chromatin switch underlying the switch of the npBAF subunit, BAF53a for the nBAF subunit BAF53b. Recapitulating this microRNA/chromatin switch in fibroblasts leads to their direct conversion to neurons. We have defined the kinetics of nBAF complex assembly in the formation of induced neurons from ES cells or fibroblasts as well as normal neural differentiation and using proteomic analysis find that this switch also includes the removal of SS18 and its replacement by CREST at mitotic exit. We find that switching of chromatin remodeling mechanisms is highly correlated with a broad switch in the use of neurogenic transcription factors. Knockdown of SS18 in neural stem cells causes cell-cycle exit and failure to self-renew, while continued expression of SS18 in neurons blocks dendritic outgrowth underlining the importance of subunit switching. Recent whole genome sequencing studies show that dominant mutations in BAF subunits underlie widely different human neurologic diseases including mental retardation, microcephally, schizophrenia, sporadic mutations in autism as well as the neurodegenerative disease, amyotrophic lateral sclerosis (ALS) also known as Lou Gehrig's disease. Using whole exome analysis of ALS trios (trio=two unaffected parents and affected child), we identified and functionally characterized two de novo mutations in CREST that function in a dominant negative mode to inhibit dendrite outgrowth in cortical and motor neurons. As the aforementioned neurological diseases arise in different neuronal types, our studies suggest that the characteristics of these diseases must be interpreted in the context of the different BAF assemblies in neurons rather than a singular mSWI/SNF complex.
- 2013Maryadele J. O'Neil, editor-in-chief ; Patricia E. Heckelman, senior associate editor ; Peter H. Dobbelaar, associate editor ; Kristin J. Roman, assistant editor ; Catherine M. Kenny, senior editorial assistant ; Linda S. Karaffa, technical assistant.
- 2008 SpringerPatrice Dion, Chandra Shekhar Nautiyal, editors ; foreword by John D. Rummel.
- 2008 SpringerDavid R. Anderson.
- 2007 SpringerWerner Krabs, Stefan Wolfgang Pickl.
- 2006 SpringerJörg Overmann (ed.).Syntrophic associations in methanogenic degradation / B. Schink -- Symbiosis between non-related bacteria in phototropic consortia / J. Overmann -- Prokaryotic symbionts of termite gut flagellates : phylogenetic and metabolic implications of a tripartite symbiosis / A. Brune, U. Stingl -- Towards an understanding of the killer trait : Caedibacter endocytobionts in Paramecium / J. Kusch, H.-D. Görtz -- Bacterial ectosymbionts which confer motility : Mixotricha paradoxa from the intestine of the Australian termite Mastotermes darwiniensis / Helmut König ... [et al.] -- Extrusive bacterial ectosymbionts of ciliates / G. Rosati -- Hydrogenosomes and symbiosis / J.H.P. Hackstein, N. Yarlett -- Molecular interactions between Rhizobium and legumes / P. Skorpil, W.J. Broughton -- Molecular mechanisms in the nitrogen-fixing Nostoc-bryophyte symbiosis / J.C. Meeks -- Symbiosis of thioautotrophic bacteria with Riftia pachyptila / F.J. Stewart, C.M. Cavanaugh -- Symbioses of methanotrophs and deep-sea mussels (Mytilidae: Bathymodiolinae) / E.G. DeChaine, C.M. Cavanaugh -- Symbioses between bacteria and gutless marine oligocahetes / N. Dubilier, A. Blazejak, C. Rühland -- Roles of bacterial regulators in the symbiosis between Vibrio fischeri and Euprymna scalopes / K. Geszvain, K. Visick -- Molecular requirements for the colonization of Hirudo medicinalis by Aeromonas veronii / J. Graf.
- 2011Kartik Viswanathan.Pancreatic [Beta]-cells secrete insulin to maintain systemic glucose balance. In response to physiological or pathological stresses that increase insulin demand, [Beta]-cells proliferate and enhance insulin secretion to increase insulin output. However, the mechanisms that govern these facultative changes are unclear. In this thesis, I investigate two potential factors in achieving these essential adaptive changes -- hypoxia inducible factor 1 alpha (Hif1a) and prolactin receptor (Prlr). During pregnancy, a common acquire state with increased insulin demand, Hif1a and Hif1a target gene expression, including Vegfa, Glut1, Gck were increased in maternal islets. Using mouse genetics, conditional deletion of Hif1a in [Beta]-cells ([Beta]Hif1a KO) resulted in glucose intolerance in pregnant, but not virgin, mice. Pregnant [Beta]Hif1a KO mice had impaired target gene expression, defective islet insulin secretion, and reduced vascularity. Pregnant mice develop transient hyperlipidemia, and recapitulation of the hyperlipidemia with fat-challenge or lipid treament induced Hif1a, Vegfa, and Pgk1 expression. Similar to pregnant [Beta]Hif1a KO mice, fat-challenged [Beta]Hif1a KO developed hyperglycemia, hypoinsulinemia, and glucose intolerance. All three hyperlipidemic states show ER stress, and treatment of unstressed mouse or human islets with thapsigargin was sufficient to increase Hif1a and downstream targets. To assess the significance of prolactin signaling in [Beta]-cell function and proliferation in adaptive settings, I created a novel conditional Prlr mouse model. In non-pregnant mice with [Beta]-cell-specific deletion of Prlr ([Beta]Prlr KO), glucose homeostasis is normal. However, pregnant [Beta]Prlr KO mice developed significant glucose intolerance. Given the widespread effects of lactogens on [Beta]-cell physiology, I anticipate altered [Beta]-cell proliferation and secretion in [Beta]Prlr KO islets, and current studies are underway to address this hypothesis. Collectively, our work has revealed that in settings of insulin resistance, both Hif1a and Prlr signaling play important roles in regulating the physiological changes required for proper [Beta]-cell function.
- 2006 SpringerWilhelm Foissner, Kuidong Xu.vol. 1. Protospathidiidae, Arcuospathidiidae, Apertospathulidae --
- 2006 Springerby Helmut Berger.
- 2008 Springerby John B. Silver.Designing a mosquito sampling programme -- Sampling the egg population -- Sampling the larval population -- Sampling the emerging adult population -- Sampling the adult resting population -- Sampling adults by animal bait catches and by animal-baited traps -- Blood-feeding and its epidemiological significance -- Sampling adults with non-attractant traps -- Sampling adults with light-traps -- Sampling adults with carbon dioxide traps -- Sampling adults with visual attraction traps, sound traps, and other miscellaneous attraction traps -- Estimation of the mortalities of the immature stages -- Methods of age-grading adults and estimation of adult survival rates -- Estimating the size of the adult population -- Measuring adult dispersal -- Experimental hut techniques -- Indices of association and species diversity indices.
- 2007 Springerguest editors, James S. Diana, Terry L. Margenau.
- 2006 SpringerJim O. Vigoreaux.
- 2015 SpringerDavid J. K. Balfour, Marcus R. Munafò, editors.The primary purpose of this book and its companion volume The Neuropharmacology of Nicotine Dependence is to explore the ways in which recent studies on nicotine and its role in tobacco addiction have opened our eyes to the psychopharmacological properties of this unique and fascinating drug. While the present volume considers the molecular and genetic factors which influence behavioral responses to nicotine and how these may impact on the role of nicotine in tobacco dependence, the book The Neuropharmacology of Nicotine Dependence focuses on the complex neural and psychological mechanisms that mediate nicotine dependence in experimental animal models and their relationship to tobacco addiction in humans. These volumes will provide readers with a contemporary overview of current research on nicotine psychopharmacology and its role in tobacco dependence from leaders in this field of research and will hopefully prove valuable to those who are developing their own research programmes in this important topic. CTBN Vol 23 The Behavioral Genetics of Nicotine and Tobacco The primary purpose of this book and its companion volume The Neuropharmacology of Nicotine Dependence is to explore the ways in which recent studies on nicotine and its role in tobacco addiction have opened our eyes to the psychopharmacological properties of this unique and fascinating drug. While the present volume considers the molecular and genetic factors which influence behavioral responses to nicotine and how these may impact on the role of nicotine in tobacco dependence, the book The Neuropharmacology of Nicotine Dependence focuses on the complex neural and psychological mechanisms that mediate nicotine dependence in experimental animal models and their relationship to tobacco addiction in humans. These volumes will provide readers with a contemporary overview of current research on nicotine psychopharmacology and its role in tobacco dependence from leaders in this field of research and will hopefully prove valuable to those who are developing their own research programmes in this important topic.
- 2014 CRCnetBASEedited by Carla Mucignat-Caretta, Department of Molecular Medicine, University of Padova, Padova, Italy.Chapter 1. Introduction to chemical signaling in vertebrates and invertebrates / Tristram D. Wyatt -- chapter 2. Pheromones and general odor perception in insects / Michel Renou -- chapter 3. First investigation of the semiochemistry of South African dung beetle species / Barend (Ben) Victor Burger -- chapter 4. Pheromone reception in insects : the example of silk moths / Karl-Ernst Kaissling -- chapter 5. Chemical communication in the honey bee society / Laura Bortolotti and Cecilia Costa -- chapter 6. Drosophila pheromones : from reception to perception / Wynand van der Goes van Naters -- chapter 7. How drosophila detect volatile pheromones : signaling, circuits, and behavior / Samarpita Sengupta and Dean P. Smith -- chapter 8. Chemical signaling in amphibians / Sarah K. Woodley -- chapter 9. Vomeronasal organ : a short history of discovery and an account of development and morphology in the mouse / Carlo Zancanaro -- chapter 10. Vomeronasal receptors and signal transduction in the vomeronasal organ of mammals / Simona Francia, Simone Pifferi, Anna Menini, and Roberto Tirindelli -- chapter 11. Central processing of intraspecific chemical signals in mice / Carla Mucignat-Caretta -- chapter 12. Molecular and neural mechanisms of pheromone reception in the rat vomeronasal system and changes in the pheromonal reception by the maturation and sexual experiences / Makoto Kashiwayanagi -- chapter 13. Social cues, adult neurogenesis, and reproductive behavior / Paolo Peretto and Raúl G. Paredes -- chapter 14. Influence of cat odor on reproductive behavior and physiology in the house mouse (mus musculus) / Vera V. Voznessenskaya -- chapter 15. Pheromone of tiger and other big cats / Mousumi Poddar-Sarkar and Ratan Lal Brahmachary -- chapter 16. Cattle pheromones / Govindaraju Archunan, Swamynathan Rajanarayanan, and Kandasamy Karthikeyan -- chapter 17. Pheromones for newborns / Benoist Schaal -- chapter 18. Pheromone processing in relation to sex and sexual orientation / Ivanka Savic -- chapter 19. Human pheromones : do they exist? / Richard L. Doty.
- 2014 SpringerSusan L. Andersen, Daniel S. Pine, editors."Brain development, whether typical or atypical, provides the foundation for all behavior and possible psychopathology. This volume takes a comprehensive and translational approach in describing how basic neuronal patterning occurs, organizes into systems, and forms functional and inter-connected networks. The role that hormones and genes play in influencing brain development is also described. The resultant emotional, cognitive, reward, and social systems show how typical development proceeds. The second part of the book describes when the process of development goes awry. Here, a number of childhood disorders are covered. Autism, ADHD, emotional syndromes of bipolar disorder and depression, oppositional defiant disorder and conduct disorder, and sleep and schizophrenia are included in this volume. Written by leading experts in their respective fields, this volume will be a valuable resource to mental health professionals as well as preclinical investigators hoping to gain additional understanding about the neurobiology of the developing brain."--Publisher's website.
- 2012 ScienceDirectedited by Andries Kalsbeek, Martha Merrow, Till Roenneberg, and Russell G. Foster.This issue of Progress in Brain Research reviews current knowledge and understanding, provides a starting point for researchers and practitioners entering the field, and builds a platform for further research and discovery. Leading authors review the state-of-the-art in their field of investigation, and provide their views and perspectives for future research Chapters are extensively referenced to provide readers with a comprehensive list of resources on the topics covered All chapters include comprehensive background information and are written in a clear form that is also accessible to the non-specialist.
- 2006 Springer[edited by] J. Fitter, T. Gutberlet, J. Katsaras.
- New perspectives in the study of Mesoamerican primates : distribution, ecology, behavior, and conservation2006 Springeredited by Alejandro Estrada ... [et al.].Overview of the Mesoamerican primate fauna, primate studies, and conservation concerns / Alejandro Estrada ... [et al.] -- Taxonomy and distributions of Mesoamerican primates / Anthony B. Rylands ... [et al.] -- The biogeographic history of Mesoamerican primates / Susan M. Ford -- Demographic features of Alouatta pigra populations in extensive and fragmented forests / Sarie Van Belle and Alejandro Estrada -- Population structure of black howlers (Alouatta pigra) in southern Belize and responses to Hurricane Iris / Mary S.M. Pavelka and Colin A. Chapman -- The effects of forest fragment age, isolation, size, habitat type, and water availability on monkey density in a tropical dry forest / Holly Noelle DeGama-Blanchet and Linda Marie Fedigan -- Forest fragmentation and its effects on the feeding ecology of black howlers (Alouatta pigra) from the Calakmul area in Mexico / Andrómeda Rivera and Sophie Calmé -- Intestinal parasitic infections in Alouatta pigra in tropical rainforest in Lacandona, Chiapas, Mexico : implications for behavioral ecology and conservation / Kathryn E. Stoner and Ana González Di Pierro -- Average body weight for mantled howling monkeys (Alouatta palliata) : an assessment of average values and variability / Kenneth E. Glander -- An exploratory analysis of developmental plasticity in Costa Rican mantled howler monkeys (Alouatta palliata palliata) / Clara B. Jones -- Travel patterns and spatial mapping in Nicaraguan mantled howler monkeys (Alouatta palliata) / Paul A. Garber and Petra E. Jelinek -- Use of landmark cues to locate feeding sites in wild capuchin monkeys (Cebus capucinus) : an experimental field study / Paul A. Garber and Ellen Brown -- Leap, bridge, or ride? Ontogenetic influences on positional behavior in Cebus and Alouatta / Michelle F. Bezanson -- Food choice by juvenile capuchin monkeys (Cebus capucinus) in a tropical dry forest / Katherine C. MacKinnon -- Why be alpha male? Dominance and reproductive success in wild white-faced capuchins (Cebus capucinus) / Katharine M. Jack and Linda M. Fedigan -- Post-conceptive mating in white-faced capuchins, Cebus capucinus : hormonal and sociosexual patterns of cycling, noncycling, and pregnant females / Sarah D. Carnegie, Linda M. Fedigan and Toni E. Ziegler -- Growth of a reintroduced spider monkey (Ateles geoffroyi) population on Barro Colorado Island, Panama / Katharine Milton and Mariah E. Hopkins -- Primates in agroecosystems : conservation value of some agricultural practices in Mesoamerican landscapes / Alejandro Estrada ... [et al.] -- Primate populations in the protected forests of Maya archaeological sites in southern Mexico and Guatemala / Alejandro Estrada ... [et al.] -- Mapping primate populations in the Yucatan Peninsula, Mexico : a first assessment / Juan Carlos Serio-Silva, Víctor Rico-Gray and Gabriel Ramos-Fernández -- A metapopulation approach to conserving the howler monkey in a highly fragmented landscape in Los Tuxtlas, Mexico / Salvador Mandujano ... [et al.] -- Quantifying fragmentation of black howler (Alouatta pigra) habitat after Hurricane Iris (2001), southern Belize / Shelley M. Alexander, Mary S.M. Pavelka and Nicola H. Bywater -- New perspectives in the study of Mesoamerican primates : concluding comments and conservation priorities / Paul A. Garber, Alejandro Estrada and Mary S.M. Pavelka.
- 2008 CRCnetBASEedited by Quentin D. Wheeler.Introductory : toward the new taxonomy / Quentin D. Wheeler -- Networks and their role in e-taxonomy / Malcolm J. Scoble -- Taxonomy as a team sport / Sandra Knapp -- Planetary biodiversity inventories as models for the new taxonomy / Lawrence M. Page -- On the use of taxonomic concepts in support of biodiversity research and taxonomy / Nico M. Franz, Robert K. Peet and Alan S. Weakley -- International infrastructure for enabling the new taxonomy : the role of the Global Biodiversity Information Facility (GBIF) / Larry Speers and James L. Edwards -- DNA sequence in taxonomy : opportunities and challenges / Rudolf Meier -- Animal names for all : ICZN, ZooBank and the new taxonomy / Andrew Polaszek, Richard Pyle and Doug Yanega -- Understanding morphology in systematic contezxt : three-dimensional specimen ordination and recognition / Norman MacLeod -- Taxonomic shock and awe / Quentin D. Wheeler.
- 2013 ScienceDirectedited by Lisa Plitnick, Danuta Herzyk.Machine generated contents note: I.Development of Biophar-Maceuticals Defined as Novel Biologics -- 1.Overview of Biopharmaceuticals and Comparison with Small-molecule Drug Development / Thomas R. Gelzleichter -- Introduction -- History and Evolution of Biopharmaceuticals -- Development of Diverse Biopharmaceutical Modalities -- Comparison of Small-Molecule Drugs to Biopharmaceuticals -- Summary -- References -- 2.Regulatory Guidelines and their Application in the Nonclinical Evaluation of Biological Medicines / Maggie Dempster -- Introduction -- Species Selection -- Study Design Considerations for Repeat-Dose Studies -- Immunogenicity -- Reproductive and Developmental Toxicity -- Genotoxicity and Carcinogenicity -- Special Considerations for Anticancer Drugs -- First-in-Human (Fih) Clinical Trial -- Summary -- References -- 3.Early De-risking Strategy for Novel Biotherapeutics / Beth Hinkle -- Introduction -- Establishing A Safety Profile for Biotherapeutics -- General Safety Considerations Related to Biotherapeutics -- Progress in Evaluation of Immunotoxicity -- Can We Better Address Potential Off-Target Toxicity? -- Summary -- References -- 4.Novel Biopharmaceuticals: Pharmacokinetics, Pharmacodynamics, and Bioanalytics / Wolfgang Seghezzi -- Introduction -- Absorption, Distribution and Elimination of Biopharmaceuticals -- Disposition of Modified Molecules -- "Metabolism" and Biodistribution for Biopharmaceuticals -- Immunogenicity and impacts on PK and biodistribution -- Pharmacokinetics and Pharmacodynamics -- Preclinical to Clinical Translation -- Bioanalytics -- Drug Assays -- Biomarkers: Target Engagement Assays -- Immunogenicity Assessment: ADA Assays -- Summary -- References -- II.Development of Biosimilars -- 5.Overview of Biosimilar Therapeutics / Danuta J. Herzyk -- Introduction -- The Concept of Biosimilars -- General Considerations for Development of Biosimilars -- Biosimilar Candidates Based on Modality and Therapeutic Class -- Summary -- References -- 6.Regulatory Standards for the Approval of Biosimilar Products: A Global Review / Barbara Mounho-Zamora -- Introduction -- European Union -- Pioneer for the First Regulatory Pathway for Biosimilar Products -- The World Health Organization Guidance on Biosimilars -- Regulatory Pathway for Biosimilar Products in the United States -- Biosimilar Pathways in Other Regions -- Summary -- References -- 7.Early Characterization of Biosimilar Therapeutics / Thomas R. Gelzleichter -- Introduction -- Recombinant Insulins -- Recombinant Human Growth Hormone -- Recombinant Erythropoietins -- Recombinant Granulocyte Colony-Stimulating Factor -- Recombinant Interferons -- Low Molecular Weight Heparins -- Monoclonal Antibodies -- Other Classes -- Summary -- References -- III.Vaccines -- 8.Introduction to Vaccines and Adjuvants / Deborah L. Novicki -- Introduction -- The History of Vaccines -- The Impact of Vaccines on Human Health -- Advancements in Vaccines Technologies -- Advancements in Adjuvant Technologies -- Approved Infectious Disease Vaccines and Diseases for Which Preventive Vaccines are Still Needed -- Therapeutic Vaccines -- Product Complexity From a Quality Perspective -- Nonclinical Testing -- Clinical Testing -- Vaccine Development Using the Animal Rule -- The Anti-Vaccines Movement and Misperceptions About Vaccines and Their Safety -- Summary -- References -- 9.Global Regulatory Guidelines for Vaccines / Lisa M. Plitnick -- Introduction -- Toxicity Assessment -- Additional Toxicity Assessments -- Special Considerations -- Formulation/Delivery Devices -- Alternate Routes of Administration -- Special Considerations for Particular Types of Vaccines -- Summary -- References -- 10.Special Considerations for the Nonclinical Safety Assessment of Vaccines / Jayanthi J. Wolf -- Introduction -- De-risking Strategies for Vaccines -- Pharmacokinetics and Pharmacodynamics Assessments -- Differences in the Nonclinical Safety Assessment of Vaccines and Biopharmaceutical Drugs -- Summary -- References -- IV.Specialty Biologics and Indications -- 11.Turning the Corner with Viral-based Gene Therapy -- Development of the Rogue Biopharmaceutical / Timothy K. Maclachlan -- Introduction -- A History and Primer of Gene Therapy -- The Process of Getting Genes into Cells -- Health Authority Regulation of Gene Therapy Products -- Nonclinical Safety Studies and Regulatory Guidance -- Summary -- References -- 12.Blood Products / Vikram Arora -- Introduction -- Plasma-Derived Blood Products -- Blood Products Derived from Recombinant Technologies -- Summary -- References -- 13.Biological Therapies for Cancer / Gautham K. Rao -- Introduction -- Global Regulatory Guidances -- General Principles of Toxicology Assessments -- Nonclinical Studies and Principles of Study Design -- Specialty Toxicology Assessments -- Pharmacokinetics, Immunogenicity, and Pharmacodynamics -- Nonclinical Development of Marketed Biologies -- Recombinant Analogs of Endogeneous Human Proteins (Interferons, Cytokines, Enzymes) -- Summary -- References -- 14.Nonclinical Development of Multi-targeting Biopharmaceuticals / Anu V. Connor -- Introduction -- Scientific Rationale for Multi-Targeting Biopharmaceuticals (MTBs) -- Evolution of Technology Platforms -- General Challenges and Considerations -- Summary of Challenges -- T Cell-Dependent Bispecific Antibody (TDB) Biotherapeutics -- Dual-Targeting Antibodies -- Summary -- References -- 15.Considerations in the Development of Pluripotent Stem Cell-based Therapies / Joy A. Cavagnaro -- Introduction -- Early Decisions -- Nonclinical Development -- Translating Preclinical Data to Clinical Application -- Global Regulatory Guidance -- Summary -- References.
- 2007 SpringerPetra Marschner and Zdenko Rengel (eds.)
- 2008 Springerby Andrii Rozhok.
- 2008 SpringerGiuseppe Valacchi, editor ; Paul A. Davis, co-editor.
- 2007 ScienceDirectedited by Helmut Sies and Bernard Brüne.Also available: Print – 2007
- 2006 Springeredited by Giuseppe Cicco... [et al.].Also available: Print – 2006
- 2008 Springeredited by Lars Olof Björn.
- 2013 SpringerJohn A. Fuerst, editor.History, classification and cultivation of the planctomycetes / Cheryl Jenkins and James T. Staley -- Cell compartmentalization and endocytosis in planctomycetes: structure and function in complex bacteria / John A. Fuerst, Richard I. Webb, and Evgeny Sagulenko -- Structural aspects of MC proteins of PVC superphylum members / Damien P. Devos -- Cell biology of anaerobic ammonium-oxidizing bacteria: unique prokaryotes with an energy-conserving intracellular compartment / Sarah Neumann, Muriel C.F. van Teeseling and Laura van Niftrik -- Acidophilic planctomycetes: expanding the horizons of new planctomycete diversity / Svetlana N. Dedysh and Irina S. Kulichevskaya -- Toward the development of genetic tools for Planctomycetes / Mareike Jogler and Christian Jogler -- Genomics and bioinformatics of the PVC superphylum / Olga K. Kamneva, Daniel H. Haft, Stormy J. Knight, Davide A. Liberles, and Naomi L. Ward -- Distribution and evolution of C1 transfer enzymes and evolution of the planctomycetes / Ludmila Chistoserdova -- Unusual members of the PVC superphylum: the methanotrophic Verrucomicrobia genus "Methylacidiphilum" / Christine E. Sharp, Huub J.M. Op den Camp, Ivica Tamas, and Peter F. Dunfield -- Phyla related to Planctomycetes: members of phylum Chlamydiae and their implications for Planctomycetes cell biology / Claire Bertelli and Gilbert Greub -- Planctomycetes: their evolutionary implications for models for origins of eukaryotes and the eukaryote nucleus and endomembranes / John A. Fuerst and Evgeny Sagulenko -- Final word: the future of planctomycetology and related studies / John A. Fuerst -- Index.
- 2008 SpringerBerryman, A. A.; Berryman, A. A.; Kindlmann, Pavel.
- 2007 Springerby Ragan M. Callaway.
- 2007 SpringerUta B. Schambra
- 2006 Springer[edited by] Shawn M. Lehman, John G. Fleagle.Biogeography and primates : a review / Shawn M. Lehman, John G. Fleagle -- Nested distribution patterns and the historical biogeography of the primates of Guyana / S.M. Lehman -- Genetic evidence on the historical biogeography of Central American howler monkeys / Julie A. Ellsworth, Guy A. Hoelzer -- Ecological biogeography of primates in Guyana / S.M. Lehman ... [et al.] -- Contrasting phylogeographic histories of chimpanzees in Nigeria and Cameroon : a multi-locus genetic analysis / M. Katherine Gonder, Todd R. Disotell -- Geographic variation in Savanna baboon (Papio) ecology and its taxonomic and evolutionary implications / Jason M. Kamilar -- Biogeography and evolution of the Cercocebus-Mandrillus clade : evidence from the face / W. Scott McGraw, John G. Fleagle -- Mouse lemur phylogeography revises a model of ecogeographic constraint in Madagascar / Anne D. Yoder, Kellie L. Heckman -- Abiotic and biotic factors as predictors of species richness on Madagascar / Nancy J. Stevens, Patrick M. O'Connor -- The geography of mammals and rivers in mainland Southeast Asia / Erik Meijaard, Colin Peter Groves -- Primate biogeography and ecology on the Sunda Shelf islands : a paleontological and zooarchaeological perspective / Terry Harrison, John Krigbaum, Jessica Manser -- The biogeography of primate evolution : the role of plate tectonics, climate and chance / John G. Fleagle, Christopher C. Gilbert -- Biogeographic origins of primate higher taxa / Christopher P. Heesy, Nancy J. Stevens, Karen E. Samonds -- Mammalian biogeography and anthropoid origins / K. Christopher Beard -- Continental paleobiogeography as phylogenetic evidence / James B. Rossie, Erik R. Seiffert.
- 2006 Springeredited by Nicholas E. Newton-Fisher ... [et al.].Taxonomy and biogeography of the primates of western Uganda / Colin Groves -- Factors influencing variation in the population densities of Colobus guereza within selectively logged forest at the Budongo Reserve : the importance of lianas during a subsistence diet / Graham Anthony Preece -- How does the golden monkey of the Virungas cope in a fruit-scarce environment? / Dennis Twinomugish ... [et al.] -- The diet of olive baboons (Papio anubis) in Budongo Forest Reserve, Uganda / Adam Alberts Okecha and Nicholas E. Newton-Fisher -- Aspects of diet, foraging, and seed predation in Ugandan forest baboons / James D. Paterson -- The interaction of hormones with ecological factors in male Budongo Forest chimpanzees / Sally B. Seraphin, Patricia L. Whitten, and Vernon Reynolds -- The role of diet in self-medication among chimpanzees in the Sonso and Kanyawara communities, Uganda / Paula Pebsworth, Sabrina Krief, and Michael A. Huffman -- Geophagy in chimpanzees (Pan troglodytes schweinfurthii) of the Budongo Forest Reserve, Uganda : a multidisciplinary study / Mnason Tweheyo ... [et al.] -- Nutritional aspects of the diet of wild gorillas : how do Bwindi gorillas compare? / Jessica M. Rothman ... [et al.] -- The parasites of the gorillas in Bwindi Impenetrable National Park, Uganda / Jessica M. Rothman ... [et al.] -- Preliminary GIS analysis of range use by sympatric mountain gorillas and chimpanzees in Bwindi Impenetrable National Park, Uganda / J. Bosco Nkurunungi and Craig B. Stanford -- Comparison of sex differences in gregariousness in fission-fusion species : reducing bias by standardizing for party size / Melissa Emery Thompson and Richard W. Wrangham -- Urinary estrone conjugates and reproductive parameters in Kibale (Kanyawara) and Budongo (Sonso) chimpanzees / Melissa Emery Thompson, Richard W. Wrangham, and Vernon Reynolds -- Frequent copulations by females and high promiscuity in chimpanzees in the Kalinzu Forest, Uganda / Chie Hashimoto and Takeshi Furuichi -- Effects of injury on the locomotion of free-living chimpanzees in the Budongo Forest Reserve, Uganda / Julie Munn -- Effect of snare injuries on the fig-feeding behavior of chimpanzees of the Budongo Forest, Uganda : behavioral adaptations and long-term implications / Emma J. Stokes and Richard W. Byrne -- Deciphering junglespeak : an update on chimpanzee vocalizations, referential communication, and their relationship to language / Hugh Notman -- Instrumental leaf use by chimpanzees of the Budongo Forest (Sonso community) / Duane Quiatt -- A survey of prosimians in the national parks and forest reserves of Uganda / Lesley Ambrose -- The diets, preferences, and overlap of the primate community in the Budongo Forest Reserve, Uganda : effects of logging on primate diets / Andrew J. Plumptre -- Behavioral patterns of colobus in logged and unlogged forests : the conservation value of harvested forests / Colin A. Chapman, Michael D. Wasserman, and Thomas R. Gillespie -- Threats to, and protection of, the chimpanzees of the Budongo Forest Reserve / Vernon Reynolds -- Gorillas living on the edge : literally and figuratively / Michele L. Goldsmith, Joel Glick, and Evarist Ngabirano -- Local ecological perceptions of chimpanzees and forest resources : a case study near Budongo Forest, Uganda / Cristy Watkins.
- v. 2-15, 1995-2004. ScienceDirect
- 2007 Springeredited by Almaz Aldashev, Robert Naeije.
- 2014 SpringerJaroslava Halper, editor.This volume is a reference handbook focusing on diseases like Marfan syndrome, Ehlers-Danlos syndrome, Loeys-Dietz syndrome and other heritable soft connective tissue diseases. The book presents detailed information for both basic scientists and for clinicians seeing patients. It is also a stepping stone for new investigations and studies that goes beyond the facts about the composition and biochemistry of the connective tissue and extracellular matrix, as the authors connect individual components to specific aspects of various soft tissue disorders and to the actual or potential treatment of them. Progress in Heritable Soft Connective Tissue Diseases features very prominent physicians and scientists as contributors who bring their most recent discoveries to the benefit of readers. Their expertise will help clinicians with proper diagnosis of sometimes elusive and uncommon heritable diseases of soft connective tissues. This book also offers an update on the pathophysiology of these diseases, including an emphasis on unifying aspects such as connections between embryonic development of the different types of connective tissues and systems, and the role of TGF-beta in development and physiology of soft tissues. This new set of data explains, at least in part, why many of these disorders are interconnected, though the primary pathophysiological events, such as gene mutations, may be different for each disorder.Also available: Print – 2014
- 2012 SpringerJ. Robin Harris, editor.Introduction and technical survey: Protein aggregation and fibrillogenesis -- Fibril formation by short synthetic peptides -- In vitrooligomerization and fibrillogenesis of amyloid-beta peptides -- Tau fibrillogenesis -- Prion protein aggregation and fibrillogenesisin vitro -- Alpha-synuclein aggregation and modulating factors -- Pathological self-aggregation of [beta](2)-microglobulin: A challenge for protein biophysics -- Islet amyloid polypeptide: Aggregation and fibrillogenesisin vitroand its inhibition -- Mechanisms of transthyretin aggregation and toxicity -- Fibrillogenesis of huntingtin and other glutamine containing proteins -- Aggregation and fibrillogenesis of proteins not associated with disease: a few case studies -- Experimental inhibition of peptide fibrillogenesis by synthetic peptides, carbohydrates and drugs -- Experimental inhibition of fibrillogenesis and neurotoxicity by amyloid-beta (abeta) and other disease-related peptides/proteins by plant extracts and herbal compounds -- Alzheimer's disease -- Modeling the polyglutamine aggregation pathway in Huntington's disease: from basic studies to clinical applications -- Parkinson's disease -- Human prion diseases: From kuru to variant Creutzfeldt-Jakob disease -- Animal prion diseases -- [beta](2)-Microglobulin amyloidosis -- Systemic AA amyloidosis -- Familial amyloidotic polyneuropathy and transthyretin -- The challenge of systemic immunoglobulin light-chain amyloidosis (AL).
- 2008 SpringerRosa Margesin ... [et. al.], editors.
- 2013 CRCnetBASEJerome Goddard."Increasing problems of emerging and re-emerging infectious diseases, brought about, at least in part, by vectors fueled by increased worldwide travel and commerce, make this a highly timely book for anyone in the public practice of medical and veterinary entomology, including entomologists, public health officials, and pest control technicians and managers. Employing a reader-friendly style with bulleted texts, scenarios, and case history boxes, it targets the major vectors and pests of medical and public health importance, addresses regulatory and academic medical entomology, and discusses disease vector control with worldwide application"--Provided by publisher.
- 2012Stefanie Duttler.One of the most critical times in the life of a protein is during its biogenesis at the ribosome. A nascent polypeptide cannot achieve its final fold while still bound to the ribosome, and is at risk of being accessed by the cellular quality control machinery. However, the extent to which cotranslational ubiquitination and degradation occur remains under debate. Conflicting reports exist, ranging from 40% of nascent chains being subject to cotranslational degradation to claims that nascent polypeptides are, on the contrary, protected from degradation. Here, we developed a direct and quantitative method to determine the extent of cotranslational ubiquitination using ribosome isolation coupled to ubiquitin-affinity purification. We find that cotranslational ubiquitination occurs at low levels, and that at least a fraction of ubiquitinated nascent chains is targeted to the proteasome for degradation. We determined which proteins are susceptible to cotranslational ubiquitination and find that intrinsic sequence features determine the cotranslational ubiquitination of a specific subset of proteins. These proteins are more aggregation-prone, highly expressed and longer than 400 amino acids. Short proteins seem to be protected from cotranslational ubiquitination. One such mechanism could be the cotranslational formation of folded structures, which lead us to disrupt cotranslational folding by deleting chaperones or through drugs such as azetidine-2-carboxylic acid (AZC). Both lead to an increase of cotranslational ubiquitination as observed by pulse-labeling as well as microarray analysis. Ribosome-associated chaperones and cotranslational folding seem to have evolved to prevent a high degree of nascent chain degradation/ubiquitination. Similarly to ribosome-associated chaperones, the ubiquitin-proteasome-system may also be able to access nascent protein chains, but rather target them for degradation. We therefore determined which parts of the UPS mediate cotranslational ubiquitination. A clear E2-E3 relationship could however not be established, possibly due to redundancy in the cellular ubiquitin ligase network. Finally, we find that disruption of mRNA quality control components also leads to enhanced ubiquitination of nascent chains, suggesting that cotranslational quality control serves to avoid the production of erroneous proteins and protect the cell from resulting toxicity.
- 2014 Springer Protocolsedited by Adriana Fontes, Beate Saegesser Santos.
- 2009 SpringerSamantha J. Richardson, Vivian Cody [editors].
- 2007 CRCnetBASEedited by Trevor R. Hodkinson, John A.N. Parnell.section A. Introduction and general context -- section B. Reconstructing and using the tree of life -- section C. Taxonomy and systematics of species rich groups (case studies).
- Regulable and cell-type-specific rescue of Niemann-pick disease type C, a neurodegenerative lysosomal storage disorder2011Manuel Eduardo Lopez, Jr.Niemann-Pick disease type C (NPC) is a rare metabolic lysosomal storage disorder (LSD) marked by accumulation of large quantities of cholesterol, lipids and other metabolites in cells. Though the disorder has been extensively explored using various genetic animal models, an understanding of the molecular and cellular pathology of the disease remains limited. A cell-type-specific and regulable rescue mouse model of NPC disease was engineered in order to identify the therapeutically relevant cell type and ascertain the effect of inflammation on disease progression. With the current information presented in this dissertation, a probable road map of NPC disease pathology has been drawn. The road map for NPC may also be applicable to, or act as a template for, other lysosomal storage diseases and neurodegenerative disorders with similar pathologies.
- 2013Jaclyn Geok Yueen Lim.Adult stem cells are undifferentiated cells that are capable of both self-renewal and differentiation into a variety of specialized cells. Adult stem cell lineages have been identified in several organs including skin, blood, breast, intestine, bladder, skeletal muscle, prostate, and the testes. The changes in homeostatic conditions in these organs caused by tissue turnover or injury rely heavily on the pool of adult stem cells for cell replenishment. Therefore, the activity of adult stem cells must be tightly controlled, and many of these regulations are orchestrated by the stem cell niche, the local microenvironment in which stem cells reside. The fast generation cycle and the genetic tractability of fruit flies make the Drosophila testis stem cell niche an ideal system for the study of adult stem cell regulation. The Drosophila testis maintains two types of stem cells: germline stem cells (GSCs) which give rise to sperm, and cyst stem cells (CySCs) which differentiate into cyst cells that encapsulate germ cells. The somatic cyst cell lineage has been implicated to be required at several stages of spermatogenesis and play pivotal roles in germline proliferation, survival, and differentiation. This dissertation focuses on understanding the role of the cyst cell lineage in GSC maintenance and investigating the mechanisms by which cyst cells direct early germ cell differentiation. Previously, it has been proposed that CySCs are the source of instructive self-renewal cues for GSCs. In contrast to this model, I showed that early germ cells with GSC characteristics can be maintained in the absence of CySCs and cyst cells. These germ cells failed to enter the transit-amplifying program, which is regarded as the first step of differentiation in many adult stem cell lineages. My observations suggest that cyst cells provide a pro-differentiation environment for GSCs, and that this mechanism(s) may be repressed in CySCs which indirectly allow for GSC self-renewal. Encapsulation of germ cells by cyst cells is one of the differentiation-promoting mechanisms imposed by the soma on the germline, and I have uncovered that this process requires activation of the EGFR-Ras-MEK-MAPK pathway in differentiated cyst cells. Furthermore, I also showed that repression of EGFR activation in CySCs is important in maintaining the population of GSCs and CySCs at the niche, as premature activation of the pathway resulted in displacement of GSCs from the hub by somatic cells. Preliminary studies revealed a STAT target gene, Socs36E as a negative regulator of the EGFR pathway in CySCs to prevent out-competition of GSCs by somatic cells. To understand how the somatic cyst cells germline differentiation, I performed two genetic screens: an RNAi screen of genes that caused premature germ cell differentiation when misexpressed in the soma, and a misexpression screen of genes predicted to be cell-surface and secreted proteins. Three promising candidates were identified through these screens: two ribosomal subunits, Rpl13A and RpS10a; and a septate junction component, Neurexin IV. The two ribosomal proteins may be involved in the soma to promote germ cell encapsulation. Neurexin IV however, operates non-cell autonomously in cyst cells to regulate germ cell differentiation into spermatocytes. Together, the results of this dissertation emphasize the importance and complexity of the interaction between adult stem cells and their microenvironment in maintaining tissue homeostasis.
- Regulation of the longevity associated tumor suppressor FoxO3 by lysine methylation and binding to protein partners2010Daniel Richard Calnan.The FoxO family of transcription factors plays an important role in longevity and tumor suppression by regulating the expression of a wide range of target genes. FoxO3 has recently been found to be associated with extreme longevity in humans and to regulate the homeostasis of adult stem cell pools in mammals, which may contribute to longevity. The activity of FoxO3 is controlled by a variety of post-translational modifications that have been proposed to form a 'code' affecting FoxO3 subcellular localization, DNA binding ability, protein-protein interactions and protein stability. Lysine methylation is a key post-translational modification on histones that regulate chromatin accessibility and is a key part of the 'histone code'. However, whether lysine methylation plays a role in modulating FoxO3 activity has never been examined. I found that the methyltransferase Set9 directly methylates FoxO3 in vitro and in cells. Using a combination of tandem mass spectrometry and methyl-specific antibodies, I find that Set9 methylates FoxO3 at a single residue, lysine 271, a site previously known to be deacetylated by Sirt1. Methylation of FoxO3 by Set9 decreases FoxO3 protein stability, while slightly increasing FoxO3 transcriptional activity. The modulation of FoxO3 stability and activity by methylation may be critical for fine-tuning cellular responses to stress stimuli, which may in turn affect FoxO3's ability to promote tumor suppression and longevity. Post-translational modifications control many aspects of FoxO3 activity, including protein stability, subcellular localization and binding partner association. To analyze whether FoxO3 functions primarily alone, or in a complex with other factors, I used size exclusion chromatography to assess the size of the potential FoxO3 protein complexes in cellular extracts. I found that FoxO3 is present in fractions with a relative molecular weight larger ranging from 250 to 700 kDa. Interestingly, the cytoplasmic fraction of FoxO3 appears to be in a larger complex than the nuclear fraction, suggesting that FoxO3 is present in more than one protein complex in cells. To identify novel binding partners of FoxO3 that could be present in these large molecular-weight protein complexes, I conducted a tandem affinity purification (TAP) of dual tagged FoxO3 in HeLa S3 cells followed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) of the elution from the immunoprecipitation. I identified over 1,000 potential FoxO3 binding partners that were not present in the control immunoprecipitation. These candidate binding partners included protein kinases (e.g. mTOR, MAPKs), other tumor suppressors (e.g. p65, p130) and oncogenes (e.g.Myb), as well as proteins that contain a methyl lysine (e.g. WDR17, PHF3) and acetyl lysine (e.g. BAZ2B, BRD1) binding domains. Taken together, these results suggest that FoxO3 functions in large protein complexes that could affect FoxO3 activity. The formation of FoxO3-containing complexes could be triggered by FoxO3 post-translational modifications. By increasing our understanding of the regulation of FoxO3, as well identifying possible interacting proteins, we further our knowledge of the pathways important for both longevity and age related diseases, including cancer.
- 2006 Springeredited by Larissa Swedell and Steven R. Leigh.Perspectives on reproduction and life history in baboons / Larissa Swedell & Steven R. Leigh -- Infant mortality, paternity certainty, and female reproductive strategies in hamadryas baboons / Larissa Swedell & Julian Saunders -- Female behavioral strategies of hybrid baboons in the Awash National Park, Ethiopia / Jacinta C. Beehner & Thore J. Bergman -- Hybrid baboons and the origins of the hamadryas male reproductive strategy / Thore J. Bergman -- The social and ecological flexibility of Guinea baboons : implications for Guinea baboon social organization and male strategies / Anh Galat-Luong, Gérard Galat & Suzanne Hagell -- Social organization, reproductive systems, and mediation of baboon endogenous virus (BaEV) copy number in gelada, hamadryas, and other Papio baboons / Monica Uddin, Jane E. Phillips-Conroy & Clifford J. Jolly -- Reproduction, mortality, and female reproductive success in chacma baboons of the Okavango Delta, Botswana / Dorothy L. Cheney ... [et al.] -- Maternal characteristics and offspring growth in chacma baboons : a life-history perspective / Sara E. Johnson -- Whose life is it anyway? Maternal investment, developmental trajectories, and life history strategies in baboons / Louise Barrett, S. Peter Henzi & John E. Lycett -- Ontogeny, life history, and maternal investment in baboons / Steven R. Leigh & Robin M. Bernstein -- Testicular size, developmental trajectories, and male life history strategies in four baboon taxa / Clifford J. Jolly & Jane E. Phillips-Conroy -- The evolutionary past and the research future : environmental variation and life history flexibility in a primate lineage / Susan C. Alberts & Jeanne Altmann.
- 2006 Springeredited by Alison Jolly ... [et al.].The distribution and biogeography of the ringtailed lemur (Lemur catta) in Madagascar / Steven M. Goodman, Soava V. Rakotoarisoa and Lucienne Wilmé -- A preliminary estimate of Lemur catta population density using satellite imagery / Robert W. Sussman ... [et al.] -- Berenty Reserve : a research site in southern Madagascar / Alison Jolly ... [et al.] -- Beza Mahafaly Special Reserve : a research site in southwestern Madagascar / Robert W. Sussman and Joelisoa Ratsirarson -- Plant species fed on by Lemur catta in gallery forests of the southern domain of Madagascar / Bruno Simmen ... [et al.] -- Tamarind recruitment and long-term stability in the gallery forest at Berenty, Madagascar / Kathryn Blumenfeld-Jones ... [et al.] -- Home ranges of ringtailed lemur troops and the density of large trees at Berenty Reserve, Madagascar / Naoki Koyama ... [et al.] -- The influence of tamarind tree quality and quantity on Lemur catta behavior / Anne S. Mertl-Millhollen ... [et al.] -- Feeding competition between introduced Eulemur fulvus and native Lemur catta during the birth season at Berenty Reserve, southern Madagascar / Susan Pinkus, James N.M. Smith and Alison Jolly -- Tradition and novelty : Lemur catta feeding strategy on introduced tree species at Berenty Reserve / Takayo Soma -- Diet quality and taste perception of plant secondary metabolites by Lemur catta / B. Simmen ... [et al.] -- Territory as bet-hedging : Lemur catta in a rich forest and an erratic climate / Alison Jolly ... [et al.] -- Resource defense in Lemur catta : the importance of group size / R. Ethan Pride ... [et al.] -- Social changes in a wild population of ringtailed lemurs (Lemur catta) at Berenty, Madagascar / Shinichiro Ichino and Naoki Koyama -- Obsession with agonistic power / Michael. E. Pereira -- Male and female ringtailed lemurs' energetic strategy does not explain female dominance / Hantanirina Rasamimanana ... [et al.] -- Male sociality and integration during the dispersal process in Lemur catta : a case study / Lisa Gould -- Patterns of health, disease, and behavior among wild ringtailed lemurs, Lemur catta: effects of habitat and sex / Michelle L. Sauther ... [et al.] -- Bald lemur syndrome and the miracle tree : alopecia associated with Leucaena leucocephala at Berenty Reserve, Madagascar / Graham C. Crawford ... [et al.] -- Temporal change in tooth size among ringtailed lemurs (Lemur catta) at the Beza Mahafaly Special Reserve, Madagascar : effects of an environmental fluctuation / Frank P. Cuozzo and Michelle L. Sauther.
- Role of theory in advancing 21st century biology : catalyzing transformative research : report of Committee on Defining and Advancing the Conceptual Basis of Biological Sciences in the 21st Century, National Research Council.2007 NAP
- 2012Peggie Cheung.Epigenetics is the study of heritable changes in gene expression that occur independent of changes in the primary DNA sequence. Chromatin structure defines the state in which genetic information is organized in the cell. The organization of this structure greatly influences the abilities of genes to be activated or silenced. In eukaryotic cells, 146 base pairs of DNA is wrapped around the histone octamer (two H2A/H2B dimers and one H3/H4 tetramer) forming the nucleosomes, the basic unit of chromatin. The nucleosome cores are connected by linker DNA sequences to further package into higher-order chromatin structures. In addition to the core histones, each histone contains an unstructured N-terminal tail. The histone tails are the sites of most of the post-translational modifications (PTMs), such as acetylation, methylation and phosphorylation. These modifications regulate the structure and function of chromatin through two general mechanisms. In the first model, histone modifications may play a direct role in altering chromatin structure. For example, histone acetylation neutralizes the positive charge of lysine residues and thus, affecting the interactions of the histones with DNA, transcription factors and other nucleosomes. Secondly, histone modifications can indirectly affect chromatin functions by serving as a binding platform for modular proteins and complexes. For instance, the methylation of histone H3 at lysine 9 is recognized specifically by the chromatin organization modifier (Chromo) domain of heterochromatin protein 1 (HP1), which contributes to the induction and the propagation of heterochromatin structure. Ten years ago, Strahl and Allis proposed a general idea of "histone code" hypothesis, which states that histone modifications, distinct or in combination, to form a "code" to influence chromatin structure and lead to varied transcriptional outputs. In recent years, many chromatin regulators were identified, such as the proteins that "write" or "erase" or "read" the modifications. Some chromatin regulators are expressed in a tissue-specific manner and play important roles in physiology and disease pathogenesis. For instance, the H3K27 histone methyltransferase EZH2 is overexpressed in tumors such as prostate, breast, colon, skin and lung cancer. Disruption of normal patterns of covalent histone modifications is another hallmark of cancer. One of the most characterized examples is the global reduction of the trimethylation of H4K20 and acetylation of H4K16, along with hypomethylation, at repeat sequences in many tumors. Since post-translational modifications have been shown to be important for many biological processes such as gene expression, DNA damage and repair and apoptosis, disruption of these processes has been linked to carcinogenesis and other disease pathogenesis. The discovery of reversible mutations in the epigenetic machinery makes post-translational modifications as one of the most promising and expanding fields in the current biomedical research. Methylation does not neutralize the charge of the modified residue nor does addition of methyl groups add considerable bulk, this mark is believed to create a distinct molecular architecture on histones that is recognized by specialized binding domains present within chromatin-regulatory proteins. The proteins and domains that recognize histone modifications, named "effectors" or "readers", are thought to define the functional consequences of lysine methylation by transducing molecular events at chromatin into biological outcomes. Mutations in these "readers" proteins have been shown to link to many disease pathogenesis. However, relatively few effector domains have been identified in comparison to the number of modifications present on histones and non-histone proteins. Here we developed a human epigenome peptide microarray platform (HEMP) for high-throughput discovery of chromatin effectors. We probed this platform with modification-specific antibodies and known chromatin effector domains to test the integrity of the peptides on the slides. We also screened a library of Royal Domain family members and identified three effector proteins with novel modified-histone binding activity. Hence, the development of the HEMP facilitates the identification of effector proteins and understanding of chromatin signaling networks. Multiple Myeloma (MM) is a malignancy of bone marrow plasma cells that frequently results in bone marrow destruction, bone marrow failure and death. 15% of patients with multiple myeloma is diagnosed with an immunoglobin gene, t(4; 14), translocation. MM patients carrying the t(4; 14) translocation is associated with the overexpression of WHSC1/MMSET/NSD2. NSD2 is a protein lysine methylatransferase in the nuclear receptor binding SET domain protein family. However, the molecular mechanism by which NSD2 contributes to myeloma pathogenesis is not known. Here we show that the dimethylation of histone H3 at lysine 36 (H3K36) is the principal physiological activity of NSD2. In mammalian cells, H3K36me2 normally maps to gene bodies. In t(4; 14)+ myeloma cells, overexpression of NSD2 disrupts the physiologic genomic organization of H3K36me2 which is found being dispersed throughout the genome. NSD2 expression is linked to transcription activation and H3K36me2 location at gene bodies positively correlates with transcription levels. In Myeloma cells, NSD2-mediated localized elevation of H3K36me2 induces transcription at normally inert cancer-associated genes. Catalytic activity of NSD2 confers tumor formation in xenograft model and promotes oncogenic transformation of primary cells by regulating transcriptional programs that favor oncogenesis. The BAH domain is an evolutionarily conserved chromatin-associated motif. Utilizing the HEMP, we screened several BAH domains from yeast and human for binding activity. We found that the BAH domain of human ORC1 specifically bind to H4K20me2 peptides. Structural studies show that BAH domain has an aromatic dimethyl-lysine-binding cage that interacts with the bound peptide. ORC1 is dispensable for ORC complex assembly but is necessary for loading of the complex into chromatin. The ability of ORC1 BAH domain binding to H4K20me2 is required for the efficient stabilization of ORC complex at chromatin. H4K20me2 is enriched at replication origins. Abrogation in ORC1 and H4K20me2 interactions impairs cell-cycle progression. Mutations in ORC1 BAH domain have been implicated in aetiology of Meier-Gorlin syndrome (MGS), a form of primordial dwarfism. In a zebrafish model, orc1 morphants display an MGS-like dwarfism phenotype, which can be rescued by wild type Orc1 but not ORC1 binding mutants. Zebrafish depleted with H4K20me2 also displays the MGS-like phenotype. Together, our findings reveal a new function for histone methylation signaling at chromatin in the regulation of DNA replication and organismal growth. KDM2A is the first jumonjiC domain-containing demethylase identified. We solved the co-crystal structure of KDM2A and its substrate, H3K36me2. We found that KDM2A demethylation activity is required to maintain genomic stability. We also show that KDM2A is a tumor suppressor and its demethylation activity is required for suppressing cellular transformation.
- 2010Kelly Elizabeth McCann.Efficient DNA double-strand break (DSB) repair is essential for maintaining the stability of the genome. A single long-lived DSB can cause cell lethality (Bennett et al., 1996). Humans with defects in DSB repair are sensitive to DNA damaging agents and are predisposed to developing cancers, as exemplified by such diseases as Nijmegan breakage syndrome, Ataxia telangiectasia, and breast cancer susceptibility in women with mutations in the BRCA1 and BRCA2 genes (Duker, 2002). Repair of DNA damage requires activation of cell cycle checkpoint controls, recruitment of repair proteins to DNA lesions, and transcriptional activation of relevant genes. As shown by our data, deletion of particular histone modification genes produces sensitivity to ionizing radiation in Saccharomyces cerevisiae, suggesting a role for chromatin modification enzymes in the repair process as well. Because damage recognition and repair of lesions are both influenced by chromatin structure, we began by studying the role of histone acetylation in the DNA damage response. Acetylation of the N-terminal tails of histone H4 opens the chromatin to allow repair enzymes to access broken DNA. Through a screen of the yeast deletion pool, we found that deletion of BRE1 and DOT1 genes causes sensitivity to ionizing radiation. Ubiquitination of H2B on lysine 123 (H2B-K123) by ubiquitin ligase Bre1 is necessary for methylation of H3 on lysine 79 (H3-K79) by Dot1. Through the histone modifications they catalyze, these proteins are involved in many aspects of the DNA repair response, as outlined in Sections C, D, and E. Our studies focused on homologous recombination repair defects, genome-wide expression patterns in BRE1 and DOT1 deletion mutants, an analysis of the data regarding proteins purported to bind to methylated H3-K79, and optimization of a protein purification strategy to find Dot1 binding partners. Our yeast deletion pool screen also predicted a role for N-terminal acetyltransferase complex NatB in DNA double-strand break repair. In Section F, we build a case for acetylation of DNA end-binding protein Mre11.
- Safety of biologics therapy : monoclonal antibodies, cytokines, fusion proteins, hormones, enzymes, coagulation proteins, vaccines, botulinum toxins2016 SpringerBrian A. Baldo.1: Approved Biologics Used For Therapy and their Adverse Effects -- 2: Monoclonal Antibodies: Introduction -- 3: Monoclonal Antibodies Approved for Cancer Therapy -- 4: Other Approved Therapeutic Monoclonal Antibodies -- 5: Cytokines -- 6: Fusion Proteins -- 7: Peptide Hormones -- 8: Glycoprotein Hormones -- 9: Enzymes Approved for Therapy -- 10: Blood Coagulation -- 11: Vaccines -- 12: Botulinum Neurotoxins -- 13: Biosimilars.
- 2006 Springeredited by Jianguo Wu ... [et al.].
- 2012Julie Rebecca Perlin.The nervous system connects cells throughout the body to coordinate actions and transmit signals. Critical to the nervous system are the neurons that extend axons to their targets and the glial cells that interact with these axons. Oligodendrocytes in the central nervous system and Schwann cells in the peripheral nervous system wrap axons to make the myelin sheath. Myelin is critical for enhancing the speed of action potentials as well as providing support to axons. Myelin was a critical adaptation that has allowed vertebrates to increase in size, precision of movement, and complexity. Insults to myelin cause devastating diseases, and a better understanding of the normal development of myelinating glia is necessary for improving treatment of human neuropathies. In this dissertation I investigate both well-known and novel signaling pathways and their roles in Schwann cell migration and myelination in zebrafish peripheral nerves. Before making myelin, Schwann cells must migrate along and cover the surface of a bundle of axons. Here, I demonstrate that Neuregulin 1 type III (Nrg 1 type III) is required in neurons to signal through ErbB receptors in Schwann cells for Schwann cell migration along the posterior lateral line nerve, a mechanosensory nerve. Further, ectopic expression of this signal in all neurons is sufficient to attract peripheral Schwann cells into the spinal cord. There appears to be distinct regulation of Schwann cell migration in different nerves of the peripheral nervous system, as migration of Schwann cells in motor nerves requires ErbB receptors but not the Nrg 1 type III ligand. Nrg1 type III does, however, control myelination in both sensory and motor nerves. Schwann cell migration is not only important to properly localize Schwann cells, but also to localize the lateral line nerve itself, which begins in the epidermis but then transitions across a basement membrane to the subepidermal space. As they migrate, Schwann cells degrade the basement membrane beneath the skin, which allows the nerve to transition out of the epidermis, and then rebuild the basement membrane after the nerve has been repositioned and protected from the disorganization that otherwise would take place if it remained in the epidermis. Finally, through analysis of a mutation that was found in a forward genetic screen for genes essential in myelination, I also identify a novel regulator of Schwann cell myelination. Together this work elucidates new roles of known genes in Schwann cell and nerve development and also identifies a novel gene required for peripheral myelination.
- 2007 Springeredited by Christopher J.O. Baker and Kei-Hoi Cheung.
- Serosal membranes (pleura, pericardium, peritoneum) : normal structure, development and experimental pathology2006 SpringerK.N. Michailova, K.G. Usunoff.
- 2007Jan A. Pechenik.
- Small heat shock proteins as novel anti-inflammatory therapeutics for neurological diseases : multiple sclerosis and stroke2011Sara Elaine Brownell.This thesis explores the use of small heat shock proteins as anti-inflammatory therapeutics in the context of neurological diseases, specifically multiple sclerosis (MS) and stroke. The family of small heat shock proteins (sHSPs) has been extensively studied as intracellular molecular chaperones. However, recent studies looking at the role of sHSPs in neurological diseases have demonstrated a near universal upregulation of certain sHSPs in damaged and diseased brains. Initially, it was thought that sHSPs are pathological in these disease states, but transgenic overexpression and exogenous administration of sHSPs in various experimental disease paradigms have shown just the contrary -- that sHSPs are protective, not pathological. In this thesis, I have found that members of the family of sHSPs are protective and therapeutic in mouse models of multiple sclerosis and stroke, experimental autoimmune encephalomyelitis (EAE) and middle cerebral artery occlusion (MCAO), respectively. Alpha B crystallin (cryab), a member of the sHSP family, is elevated in stroke and deficiency of cryab leads to worse disease outcome, illustrating the protective role of alpha B crystallin. Exogenous administration of small heat shock proteins, including alpha B crystallin, decreases disease severity by modulating the immune system. This leads to the conclusion that sHSPs are endogenous anti-inflammatory and neuroprotectant molecules produced after neurological disease, whose beneficial properties can be augmented when administered therapeutically.
- Solanaceae and convolvulaceae - secondary metabolites : biosynthesis, chemotaxonomy, biological and economic significance : a handbook2008 SpringerEckart Eich.
- 2012Alexander Gray Vaughan.This thesis encompasses three projects at the intersection of genetics, behavior, and neurobiology in the fruitfly, Drosophila melanogaster. The major work presented is an investigation of the afferent auditory system in D. melanogaster. The afferent auditory system of D. melanogaster arises from the Johnston's Organ in the second antennal segment, from which mechanosensory neurons project to a protocerebral region known as the Antennomechanosensory and Motor Complex (AMMC). The antennal mechanosensory system in Drosophila is used in multiple contexts, including vestibular mechanosensation, wind sensitivity, and courtship hearing. During courtship, male flies present a stereotypic pulse song as well as a hum-like sine-song to female flies; these stimuli are species-specific and critical to female receptivity. Using a large enhancer-Gal4 collection, I identify 12 candidate cell types within the AMMC, divided into 7 types of projection neurons as well as 5 types of local AMMC interneurons. I tested each of these cell types for its effect on courtship hearing by testing the effect of neuronal silencing on a) female receptivity, and b) a male locomotor response (Song-Induced Locomotion). These tests reveal that the activity of one class of projection neuron (aPN1), and one class of local interneuron (aLN(al)) are critical to courtship hearing in both male and female flies. Other cell types appear to be dispensable for courtship hearing, and may play a role in non-courtship related mechanosensation. To help confirm that these phenotypes are not specific to neuronal silencing, I also hyperactivated each cell type using the temperature-sensitive dTrpA1 reagent; these results confirm that aPN1 and aLN(al) alone are necessary for courtship hearing. Overall this work identifies a set of parallel pathways from the AMMC leading to the ventrolateral protocerebrum (VLPR), but reveal that only one of these pathways is necessary for courtship hearing. The structure of this circuit is analogous to the multi-lineage projection identified in D. melanogaster olfaction, in which a parallel pathways share a common projection but carry independent information. In addition to this work, I also discuss an investigation of the role of abnormal-chemosensory jump 6 (acj6) on escape behaviors, as well as an investigation of the expression of doublesex (dsx) in the nervous system.
- 2009John S. Wilkins.In this comprehensive work, John S. Wilkins traces the history of the idea of "species" from antiquity to today, providing a new perspective on the relationship between philosophical and biological approaches.--[Book cover].
- 2006 SpringerBrian Fry.
- 2005- SpringerLinkAlso available: Print – 2001, 2003, 2005, 2008.
- 2011Kerri A. Spilker.Specification and assembly of synapses is a highly coordinated and regulated process. Knowledge of the position and connectivity of all C. elegans neurons makes it a highly useful organism for studying the underlying mechanisms that control synapse formation. Using cell-specific promoters and fluorescently-labeled synaptic vesicle proteins, we are able to monitor synapse formation in subsets of C. elegans neurons. Close observation of synapse formation in a single posterior motorneuron (DA9) led to the identification of a mutation in the alternative splicing regulator mbl-1 that changes the synaptic pattern. The cholinergic motorneuron DA9 is required for backwards locomotion and forms ~25 synapses onto both inhibitory neurons and body wall muscles in the dorsal nerve cord (DNC) of the worm. We found that the 10 most distal synapses of DA9 fail to form in mbl-1 mutants, visualized with the synaptic vesicle-associated protein RAB-3 and the active zone proteins SYD-2/liprin-[Alpha] and UNC-10/Rim. In addition, some RAB-3 mis-localizes to the dendrite of DA9 and animals have a backwards locomotion defect consistent with a loss of synapses onto dorsal body wall muscles. mbl-1 is a member of the conserved MBNL (Muscleblind like) family of CCCH zinc-finger RNA binding proteins that regulate alternative splicing of target genes by directly binding to target mRNA. In the human disease myotonic dystrophy type 1 (DM1), a progressive muscular dystrophy, sequestration of MBNL proteins in nuclear foci leads to altered splicing of downstream genes. Mis-splicing of several genes is responsible for the muscular and cardiac symptoms present in individuals with DM1. Most work on the MBNL proteins has focused on their role in muscle morphogenesis and maintenance. However, C. elegans mbl-1 is expressed in a subset of motorneurons including DA9 and is required cell autonomously in these neurons to regulate proper synapse formation. Post-synaptic and muscle markers were unaffected in mbl-1 mutant animals. Thus, our work demonstrates that mbl-1 also functions in neurons to regulate synapse formation. In a separate set of experiments, we identified a new mutation in the coding region of the touch cell-specific beta-tubulin, mec-7(wy116) that causes a defect in synapse formation in the mechanosensory neuron PLM. Previous studies have shown that mec-7 is expressed exclusively in the six touch neurons of C. elegans and is required for sensing light touch. Our mec-7 mutation leads to a loss of synaptic vesicle accumulation at PLM synaptic sites in the ventral nerve cord and synaptic vesicles are visible at ectopic locations along the lateral axon of PLM. Localization of the synaptic proteins VAMP and GIT-1 is also defective in our mutant, but neuronal morphology is wild-type. mec-7(wy116) is mildly Mec, but other alleles of mec-7 (e1506, e1527) do not phenocopy the synaptic vesicle localization defect. mec-7(wy116) is a missense mutation that alters a highly conserved Thr at position 409 to Ile. Crystal structures of tubulin indicate that this residue is on the face of tubulin that interacts with kinesin motor. Because we see synaptic vesicles along the lateral axon of PLM, we believe that kinesin-mediated vesicle transport is less efficient in mec-7(wy116) mutants.
- 2013 ScienceDirectedited by Huimin Zhao.Synthetic Biology provides a framework to examine key enabling components in the emerging area of synthetic biology. Chapters contributed by leaders in the field address tools and methodologies developed for engineering biological systems at many levels, including molecular, pathway, network, whole cell, and multi-cell levels. The book highlights exciting practical applications of synthetic biology such as microbial production of biofuels and drugs, artificial cells, synthetic viruses, and artificial photosynthesis. The roles of computers and computational design are discussed, as well as future prospects in the field, including cell-free synthetic biology and engineering synthetic ecosystems. Synthetic biology is the design and construction of new biological entities, such as enzymes, genetic circuits, and cells, or the redesign of existing biological systems. It builds on the advances in molecular, cell, and systems biology and seeks to transform biology in the same way that synthesis transformed chemistry and integrated circuit design transformed computing. The element that distinguishes synthetic biology from traditional molecular and cellular biology is the focus on the design and construction of core components that can be modeled, understood, and tuned to meet specific performance criteria and the assembly of these smaller parts and devices into larger integrated systems that solve specific biotechnology problems. Includes contributions from leaders in the field presents examples of ambitious synthetic biology efforts including creation of artificial cells from scratch, cell-free synthesis of chemicals, fuels, and proteins, engineering of artificial photosynthesis for biofuels production, and creation of unnatural living organisms. Describes the latest state-of-the-art tools developed for low-cost synthesis of ever-increasing sizes of DNA and efficient modification of proteins, pathways, and genomesHighlights key technologies for analyzing biological systems at the genomic, proteomic, and metabolomic levels which are especially valuable in pathway, whole cell, and multi-cell applications. Details mathematical modeling tools and computational tools which can dramatically increase the speed of the design process as well as reduce the cost of development.
- 2007 Springeredited by Mohamed Al-Rubeai and Martin Fussenegger.
- 2013 SpringerMichael G. Katze, editor.First, systems biology is an inter-disciplinary approach, requiring the combined talents of biologists, mathematicians, and computer scientists. Second, systems biology is holistic, with the goal of obtaining a comprehensive understanding of the workings of biological systems. This is achieved through the acquisition of massive amounts of data by high-throughput technologies-oligonucleotide microarrays, mass spectrometry, and next-generation sequencing-and the analysis of this data through sophisticated mathematical algorithms.
- 2007 SpringerP. Bringmann, E.C. Butcher, G. Parry, B. Weiss, editors.
- 2007 CRCnetBASEedited by Andrzej K. Konopka.Basic concepts of systems biology / Andrzej K. Konopka -- Understanding through modeling : a historical perspective and review of biochemical systems theory as a powerful tool for systems biology / Eberhard O. Voit and John H. Schwacke -- Thermostatics : a poster child of systems thinking / Peter Salamon, Anna Salamon, and Andrzej K. Konopka -- Friesian epistemology / Kelley L. Ross -- Reconsidering the notion of the organic / Robert E. Ulanowicz -- The metaphor of "chaos" / Wlodzimierz Klonowksi -- Biological complexity : an engineering perspective / Stephen W. Kercel -- The von Neumann's self-replicator and a critique of its misconceptions / Stephen W. Kercel -- The mathematical structure of thermodynamics / Peter Salamon ... [et al.].
- v. 1-, 2010- SpringerChoi, Sangdun.
- 2007 MyiLibraryedited by Isidore Rigoutsos and Gregory Stephanopoulos.
- 2007 Springeredited by John A. Bissonette, Ilse Storch.
- An online journal and ebook service of the Thieme Publishing Group. The ebook package is also called Thieme Clinical Collections.
- v. 2-, 2005- SpringerBronner, Felix; Farach-Carson, Mary C.
- v.1-2=, 2014 CRCnetBASEv.2 (2014) CRCnetBASEeditor, Gian Paolo Rossini.Structured account of the existing knowledge of toxic algae, the chemistry of the toxins they produce, the effects these substances exert in humans and wildlife, as well as the strategies envisaged to protect public health and the environment. Covers recent advances in the understanding of the biology of toxin producers and the factors involved in the appearance and dynamics of harmful algae blooms, the factors affecting toxin production, the synthesis of toxins both in natural producers and by chemical means in a lab, and the toxin groups posing continuing and novel. Studies effects toxic microalgae and their poisonous products exert on living systems and how they may affect human activities.
- 2006 Springeredited by Jacques Brodeur and Guy Boivin.
- 2008 CRCnetBASEHugh Cartwright ; chapter 10, Evolvable developmental systems, contributed by Nawwaf Kharma.
- 2012Alan Hunter Shain.Pancreatic ductal adenocarcinoma, subsequently referred to as pancreatic cancer, is one of the most deadly cancers with a five-year survival of only 5%. Typical treatment regimens include surgery and chemotherapy while targeted therapies are lacking. A better understanding of the underlying cancer biology may yield novel therapeutic targets. The field of genomics has transformed our ability to study cancer. Over the past two decades, microarray and sequencing based innovations have enabled biology to be carried out at the --omic scale (discussed further in Chapter 1). This dissertation employs --omic level analysis to query the differences between the pancreatic cancer genome and the normal human genome. First, high throughput structural characterizations were performed in order to annotate the mutated, rearranged, deleted, and amplified genes across seventy pancreatic cancers. These assays identified hundreds of candidate cancer genes. Among these candidates, SMURF1 was validated to be an oncogene (Chapter 2). Furthermore, various subunits of the SWI/SNF chromatin remodeling complex were validated to be tumor suppressor genes (Chapter 3). While SMURF1 and SWI/SNF were validated individually, the other candidates from structural studies were evaluated in parallel shRNA competive growth screen. From this, we characterize nine genes further -- some previously known to play a role in cancer and some novel (Chapter 4). The sum total of all of this work is a much deeper understanding of the biology of pancreatic cancer, yielding many novel therapeutic targets and hopefully allowing scientists to gain a foothold in the fight against this disease.
- 2016 SpringerH.G. Stratmann.
- 2008 SpringerBoris Bogdan, Ralph Villiger.
- 2007 Springer Protocolsedited by Nair Sreejayan, Jun Ren.Rat carotid artery balloon injury model / David A. Tulis -- Histological and morphometric analyses for rat carotid balloon injury model / David A. Tulis -- Plaque rupture model in mice / Takeshi Sasaki, Kae Nakamura and Masafumi Kuzuya -- Immunostaining of mouse atherosclerotic lesions / Hong Lu, Debra L. Rateri and Alan Daugherty -- Surgical animal model of ventricular hypertrophy / Giuseppe Marano and Alberto U. Ferrari -- Animal models of hypertension / Brett M. Mitchell, Thomas Wallerath and Ulrich Förstermann -- Rat models of cardiac insulin resistance / Sanjoy Ghosh, Brian Rodrigues and Jun Ren -- The Isolated, perfused pseudo-working heart model / Gary J. Grover and Rajni Singh -- Altering and analyzing glucose metabolism in perfused hearts of transgenic mice / Rajakumar V. Donthi and Paul N. Epstein -- Methods in the evaluation of cardiovascular renin angiotensin aldosterone activation and oxidative stress / Camila Manrique -- Detection and quantification of apoptosis in the vasculature / Wendy A. Boivin and David J. Granville -- Endothelial progenitor cell and mesenchymal stem cell isolation, characterization, viral transduction / Keith R. Brunt, Sean R. R. Hall, Christopher A. Ward, and Luis G. Melo -- Assessment of endothelial damage/dysfunction: a focus on circulating endothelial cells / Christopher J. boos, Andrew D. Blann, and Gregory Y. H. Lip -- Evaluation of endothelial function by in vivo microscopy / Rosario Scalia -- Hapotaxis of endothelial cell migration under flow / Steve Hsu, Rahul Thakar, and Song Li -- Isolation, culture, and functional analysis of adult mouse cardiomyocytes / Ronglih Liao and Mohit Jain -- Mechanical measurement of contractile function of isolated ventricular myocytes / Loren E. Wold and Jun Ren -- Studying ischemia and reperfusion in isolated neonatal rat ventricular myocytes using coverslip hypoxia / Kelly R. Pitts and Christopher F. Toombs -- Isolation and functional studies of rat aortic smooth muscle cells / Nair Sreejayan and Xiaoping Yang -- Detection of reactive oxygen species and nitric oxide in vascular cells and tissues / Hua Cai, Sergey Dikalow, Kathy K. Griendling, and David G. Harrison -- Assessment of protein glycoxidation in ventricular tissues / Shi-Yan Li and Jun Ren -- Assessment of PI-3 kinase and Akt in ischemic heart diseases in diabetes / Takashi Matsui and Amy J. Davidoff -- Two-dimensional gel electrophoresis in platelet proteomics research / Ángel García -- Stem cell therapy in the heart and vasculature / Loren E. Wold, Wangde Dai, Joan S. Dow, and Robert A. Kloner.
- 2012 SpringerMichael G. Rossmann, Venigalla B. Rao, editors.Viruses: sophisticated biological machines / M.G. Rossmann and V.B. Rao -- F(1)-ATPase: A prototypical rotary molecular motor / K. Kinosita, Jr. -- Principles of virus structural organization / B.V. Prasad and M.F. Schmid -- Reconstructing virus structures from nanometer to near-atomic resolutions with cryo-electron microscopy and tomography / J. Chang ... [et al.] -- Contractile tail machines of bacteriophages / P.G. Leiman and M.M. Shneider -- Long noncontractile tail machines of bacteriophages / A.R. Davidson ... [et al.] -- Short noncontractile tail machines: adsorption and DNA delivery by podoviruses / S.R. Casjens and I.J. Molineux -- Infection of cells by alphaviruses / D.T. Brown and R. Hernandez -- Influenza virus entry / M. Luo -- Molecular mechanisms of HIV entry / C.B. Wilen, J.C. Tilton and R.W. Doms -- Bunyavirus: structure and replication / T.S. Guu, W. Zheng and Y.J. Tao -- Viral polymerases / K.H. Choi -- Chaperonin-mediated folding of viral proteins / Z.L. Hildenbrand and R.A. Bernal -- Building the machines: scaffolding protein functions during bacteriophage morphogenesis / P.E. Prevelige and B.A. Fane -- Bacteriophage HK97 capsid assembly and maturation / R.W. Hendrix and J.E. Johnson -- Lipid-containing viruses: Bacteriophage PRD1 assembly / S.J. Butcher, V. Manole and N.J. Karhu -- Assembly of large icosahedral double-stranded RNA viruses / M.M. Poranen and D.H. Bamford -- The papillomavirus virion: a machine built to hide molecular Achilles' heels / C.B. Buck and B.L. Trus -- Procapsid assembly, maturation, nuclear exit: dynamic steps in the production of infectious herpesvirions / G. Cardone ... [et al.] -- Assembly and architecture of HIV / B.K. Ganser-Pornillos, M. Yeager and O. Pornillos -- Condensed genome structure / L.W. Black and J.A. Thomas -- The bacteriophage DNA packaging machine / M. Feiss and V.B. Rao -- The dsDNA packaging motor in Bacteriophage o29 / M.C. Morais -- Single-molecule studies of viral DNA packaging / Y.R. Chemla and D.E. Smith -- Genome gating in tailed bacteriophage capsids / P. Tavares, S. Zinn-Justin and E.V. Orlova -- Packaging in dsRNA Viruses / L. Mindich -- Mechanism of RNA packaging motor / E.J. Mancini and R. Tuma -- Helical viruses / G. Stubbs and A. Kendall.Also available: Print – 2012
- 2010Li Ling.The mammalian nervous system consists of billions of neurons arranged into complicated networks (neuronal circuits) that mediate all aspects of brain function and behavior. A fundamental goal of neuroscience is to describe the structure of neural circuits at the level of single cells and to understand how this structure enables information acquisition, processing, storage, and ultimately the control of behavior. Over the past hundred years, the cumulative efforts of many individuals have led to the development of a wide array of neuronal labeling tools. First, in this thesis, with the ultimate goal of further expanding genetically coded labeling tools to visualize the presynaptic distribution in single neurons within the mouse brain in vivo, I developed a new genetic synaptic labeling method and used it to analyze the spatial patterning of synapses in developing and mature cerebellar granule. Second, taking the advantage of the MADM system that allows gene knockout in single cells and simultaneously labeling the cells for morphology characterization, I also utilized MADM to study the function of the Rai1 gene, heterozygosity of which results in Smith-Magenis Syndrome in human.
- 2007 Springeredited by Irene Rochlitz.Cat behaviour : social organization, communication, and development / by Sharon L. Crowell-Davis -- The assessment of welfare / by Rachel A. Casey and John W.S. Bradshaw -- The human-cat relationship / by Penny L. Bernstein --Behaviour problems and welfare / by Sarah E. Heath -- Cat overpopulation in the United States / by Philip H. Kass -- The welfare of feral cats / by Margaret R. Slater -- Housing and welfare / by Irene Rochlitz -- Disease and welfare / by Kit Sturgess -- Nutrition and welfare / by Kit Sturgess and Karyl J. Hurley -- Breeding and welfare / by Andreas Steiger.
- 2006 SpringerDan W. Urry.What sustains life? an overview -- The pilgrimage: highlights in our understanding of products and components of living organisms -- Likelihood of life's protein machines: extravagant in construction yet efficient in function -- Consilient mechanisms for diverse protein-based machines: the efficient comprehensive hydrophobic effect -- On the evolution of protein-based machines: toward complexity of structure and diversity of function -- Biology thrives near a movable cusp of insolubility -- Consilient mechanisms for the protein-based machines of biology -- Advanced materials for the future: protein-based materials with potential to sustain individual health and societal development -- Appendix 1. Mechanics of elastin: molecular mechanism of biological elasticity and its relationship to contraction / Dan W. Urry and Timothy M. Parker -- Appendix 2. Development of elastic protein-based polymers as materials for acoustic absorption / Dan W. Urry... [et al.]
- Adaptive and neutral evolutionary insights from statistical analyses of population genetic data — Integration of sensory and reward information during perceptual decision-making in lateral intraparietal cortex (LIP) (100)
- Integrative modeling for genome-wide regulation of gene expression — What sustains life? : consilient mechanisms for protein-based machines and materials (100)
- Wistar Institute of Anatomy and Biology : history, organization, purposes, equipment. — Wistar Institute of Anatomy and Biology : history, organization, purposes, equipment. (1)
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Includes cited reference searching, citation maps, and an analyze tool.
Features systematic reviews that summarize the effects of interventions and makes a determination whether the intervention is efficacious or not.
Cochrane reviews are created through a strict process of compiling and analyzing data from multiple randomized control trials to ensure comprehensiveness and reliability.Provides systematic coverage of the psychological literature from the 1800s to the present through articles, book chapters and dissertations.BMJ Clinical Evidence. A clinical information tool built around systematic reviews summarizing the current state of knowledge about prevention and treatment of clinical conditions.PIER (Physicians' Information and Education Resource) is a Web-based decision-support tool designed for rapid point-of-care delivery of up-to-date, evidence-based guidance for primary care physicians.Cochrane Central Register of Controlled Trials (CENTRAL) provides access to 300,000 controlled trials that have been identified the Cochrane Collaboration.Provides drug information targeted for patients.A continually updating drug monograph.The National Guideline Clearinghouse (NGC): A comprehensive database of evidence-based clinical practice guidelines and related documents.MedlinePlus: A repository of health information from the National Library of Medicine. Links are from trusted sites. No advertising, no endorsement of commercial companies or productsLPCH CareNotes via MicroMedex: Patient education handouts customized by LPCH clinical staffMicromedex Lab Advisor: Evidence based laboratory test informationA drug database organized by generic name, trade name and drug class.LPCH / Stanford Hospital Formulary.A goldmine of trusted consumer health information from the world's largest medical library.A trusted source of expert advice for and about kids, providing the information necessary to help patients and parents understand their unique needs.Provides patient handouts from the American Academy of Family Physician.Access to the Stanford Health Library for patients.Lane provides access to over 5,000 eBooks many of which provide helpful background material that will prepare you to better tackle primary literature.
Largest, broadest eBook package; covers all sciences, as well as technology (including software), medicine, and humanities.
In addition to covering Wiley and Springer, MyiLibrary is also the only provider for Oxford and Cambridge University Press titles. No seat restrictions.A collection of biomedical books that can be searched directly by concept, and linked to terms in PubMed abstracts.
A web-based, decision support system for infectious diseases, epidemiology, microbiology and antimicrobial chemotherapy. The database, updated weekly, currently includes 337 diseases, 224 countries, 1,147 microbial taxa and 306 antibacterial (-fungal, -parasitic, -viral) agents and vaccines.
Over 10,000 notes outline the status of specific infections within each country.
Provides online, full-text access to Springer's journal titles as well as journals from other publishers.
Subjects include: life sciences, chemical sciences, environmental sciences, geosciences, computer science, mathematics, medicine, physics and astronomy, engineering and economics. Also includes eBooks.Collection of over 8 thousand fulltext titles in engineering, math, and basic and applied biomedical research. Coverage is from 1967 to the present.A library of ebooks on a wide array of topics, digitized and made available online in conjunction with the original publishers.