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Biochemistry

  • 2010 Springer
    Borea, Pier Andrea.
  • v. 1, 3-6, Supp. 1, 1967.
    Status: Not Checked OutLane Catalog Record
    1. [Symposia I-III] -- 2. [Symposia IV-VII] -- 3. Colloquia I-XIV -- 4. General sessions A-F -- 5. General sessions G-J -- [6] Author index -- [7] Supplement I-
  • 2010 Springer
    Carlier, Marie-France.
    Part 1. Cellular Aspects -- Elementary Cellular Processes Driven by Actin Assembly: Lamellipodia and Filopodia / J. Victor Small and Klemens Rottner -- Coupling Membrane Dynamics to Actin Polymerization / Shiro Suetsugu and Tadaomi Takenawa -- Endocytic Control of Actin-based Motility / Andrea Disanza, Emanuela Frittoli, Chiara Giuliani, Francesca Milanesi and Andrea Palamidessi, et al. -- Actin in Clathrin-Mediated Endocytosis / Marko Kaksonen -- Actin Cytoskeleton and the Dynamics of Immunological Synapse / Viveka Mayya and Michael L. Dustin -- Actin-based Motile Processes in Tumor Cell Invasion / Matthew Oser, Robert Eddy and John Condeelis -- Actin-based Chromosome Movements in Cell Division / Rong Li -- Roles for Actin Dynamics in Cell Movements During Development / Minna Roh-Johnson, Jessica Sullivan-Brown and Bob Goldstein -- Part 2. Molecular Aspects -- Regulation of the Cytoplasmic Actin Monomer Pool in Actin-based Motility / Pekka Lappalainen, Maarit Makkonen and Hongxia Zhao -- From Molecules to Movement: In Vitro Reconstitution of Self-Organized Actin-based Motile Processes / Marie-France Carlier and Dominique Pantaloni -- The WASP-Homology 2 Domain and Cytoskeleton Assembly / Roberto Dominguez -- Formin-Mediated Actin Assembly / David R. Kovar, Andrew J. Bestul, Yujie Li and Bonnie J. Scott -- Visualization of Individual Actin Filament Assembly / Emmanuèle Helfer -- Movement of Cargo in Bacterial Cytoplasm: Bacterial Actin Dynamics Drives Plasmid Segregation / Dyche Mullins -- Part 3. Physical Aspects -- Protrusive Forces Generated by Dendritic Actin Networks During Cell Crawling / Ovijit Chaudhuri and Daniel A. Fletcher -- Mathematical and Physical Modeling of Actin Dynamics in Motile Cells / Anders E. Carlsson and Alex Mogilner -- Force Production by Actin Assembly: Simplified Experimental Systems for a Thorough Modeling / C. Sykes, J. Prost and J.F. Joanny.
  • 2008 Springer
    Chhabra, Deepak; Dos Remedios, Cristobal G.
  • 2012 Springer
    Chen, Chang-Hwei.
  • 2012 Springer
    Samuel, Charles E.
    ADAR proteins : structure and catalytic mechanism / Rena A. Goodman, Mark R. Macbeth and Peter A. Beal -- ADAR proteins : double-stranded RNA and Z-DNA binding domains / Pierre Barraud and Frédéric H.-T. Allain -- Editing of neurotransmitter receptor and ion channel RNAs in the nervous system / Jennifer L. Hood and Ronald B. Emeson -- Modulation of microRNA expression and function by ADARs / Bjorn-Erik Wulff and Kazuko Nishikura -- Nuclear editing of mRNA 3'-UTRs / Ling-Ling Chen and Gordon G. Carmichael -- Control of ADAR1 editing of hepatitis delta virus RNAs / John L. Casey -- Bioinformatic approaches for identification of A-to-I editing sites / Eli Eisenberg -- ADARs : viruses and innate immunity / Charles E. Samuel -- Role of ADARs in mouse development / Carl R. Walkley, Brian Liddicoat and Jochen C. Hartner -- Regulation and functions of ADAR in Drosophila / Simona Paro, Xianghua Li, Mary A. O'Connell and Liam P. Keegan.
  • 2009 Springer
    Abbracchio, M. P.; Mustafa, S. Jamal; Wilson, Constance N.
  • 2012 Springer
    Harris, Tony.
    Introduction to adherens junctions: from molecular mechanisms to tissue development and disease -- How AJs evolved -- Evolution of the cadherin-catenin complex -- How cadherins and catenins interact to assemble AJs -- Three-dimensional structure of the cadherin-catenin complex -- Biophysics of cadherin adhesion -- Adherens junction assembly -- How AJs interface with other cellular machinery -- Cytoskeleton and classical cadherin adhesions -- Immunoglobulin superfamily receptors and adherens junctions -- Signaling from the adherens junction -- Adherens junction turnover: regulating adhesion through cadherin endocytosis, degradation, and recycling -- How AJs affect cell behaviour and multicellular development -- Adherens junctions during cell migration -- Adherens junctions and cadherins in Drosophilia development -- Adherens junctions in C. elegans embryonic morphogenesis -- Cadherin function during xenopus gastrulation -- Adherens junctions in mammalian development, homeostasis and disease: lessons from mice -- How AJs affect tissue homeostasis and disease -- Adherens junctions and stem cells -- Adherens junctions and cancer -- Adherens junctions and pathogen entry.
  • 2012 CRCnetBASE
    Hunter, Ross J.; Preedy, Victor R.
    1. Acylation stimulating protein (ASP) as an adipokine / Thea Scantlebury-Manning -- 2. Adiponectin as an adipokine / Zhikui Wei and G. William Wong -- 3. Adipsin as an adipokine / Yu-Feng Zhao and Chen Chen -- 4. Adrenomedullin as a cytokine / Alexis Elias Malavazos and Gianluca Iacobellis -- 5. Angiotensinogen as an adipokine / Miki Nagase and Toshiro Fujita -- 6. Apelin as an adipokine / Despina D. Briana and Ariadne Malamitsi-Puchner -- 7. C1q/TNF-related proteins (CTRPs) as adipokines / Jonathan M. Peterson and G. William Wong -- 8. Fasting-induced adipose factor/angiopoietin-like protein 4 as an adipokine / Sander Kersten -- Interleukins as adipokines / Kirsten Prüfer Stone -- Leptin as an adipokine: important definitions and applications for cancer research / Barbara Stadterman [and others] -- Omentin as an adipokine: role in health and disease / Anne M. Lenz and Frank Diamond -- Plasminogen activator inhibitor 1 (Pai-1) as an adipokine / Francis Agyemang-Yeboah -- Resistin as an adipokine / Zhikui Wei and G. William Wong -- Retinol-binding protein 4 as an adipokine / Elmar Aigner and Christian Datz -- TNF-α as an adipokine / Theron C. Gilliland, Jr. and G. William Wong -- Transforming growth factor as an adipokine / Yutaka Yata and Terumi Takahara -- Vaspin as an adipokine: a link between obesity and metabolic alterations / Peter Kovacs, Matthias Blüher, and Gabriela Aust -- Visfatin as an adipokine / Ariadne Malamitsi-Puchner and Despina D. Briana -- Thermal stress and adipokines expression / Umberto Bernabucci, Patrizia Morera, and Loredana Basiricò -- Central nervous system roles for adiponectin in neuroendocrine and autonomic function / Ted D. Hoyda, Willis K. Samson, and Alastair V. Ferguson -- The astroglial leptin receptors and obesity / Weihong Pan [and others] -- Adipokines and obesity / Alberto O. Chavez and Devjit Tripathy -- Nonalcoholic fatty liver disease and adipokines / Stergios A. Polyzos, Jannis Kountouras, and Christos Zavos -- Endocrine disruptors, adipokines, and the metabolic syndrome / Eric R. Hugo and Nira Ben-Jonathan -- Adipokines and the heart / Gianluca Iacobellis -- Adipokines as mediators of rheumatic disease / Deborah M. Levy and Earl D. Silverman -- Adipokines and allergy / Giorgio Ciprandi [and others] -- Adipokines and sleep disorders / Kapil Dhawan [and others] -- Adipokines in children and adolescents and implications for disease / HuiLing Lu and Katherine Cianflone -- Role of leptin in immune cell development and innate immunity / Pamela J. Fraker and Afia Naaz -- Visfatin and hypocaloric diets / D.A. de Luis and R. Aller -- Apelin in energy metabolism / Isabelle Castan-Laurell and Philippe Valet -- Adiponectin deficiency / Wayne Bond Lau [and others] -- Adiponectin enhances inflammation in rheumatoid synovial fibroblasts and chondrocytes / Natsuko Kusunoki, Kanako Kitahara, and Shinichi Kawai -- Resistin, imflammation and seminal fluid / Uwe Paasch [and others] -- Resistin in amniotic fluid / Shali Mazaki-Tovi [and others].
  • 2014 Springer
    Darie, Costel C.; Woods, Alisa G.
    "This volume explores the use of mass spectrometry for biomedical applications. Chapters focus on specific therapeutic areas such as oncology, infectious disease and psychiatry. Additional chapters focus on methodology as well as new technologies and instrumentation. This volume provides readers with a comprehensive and informative manual that will allow them to appreciate mass spectrometry and proteomic research but also to initiate and improve their own work. Thus the book acts as a technical guide but also a conceptual guide to the newest information in this exciting field. Mass spectrometry is the central tool used in proteomic research today and is rapidly becoming indispensable to the biomedical scientist. With the completion of the human genome project and the genomic revolution, the proteomic revolution has followed closely behind. Understanding the human proteome has become critical to basic and clinical biomedical research and holds the promise of providing comprehensive understanding of human physiological processes. In addition, proteomics and mass spectrometry are bringing unprecedented biomarker discovery and are helping to personalize medicine"--Publisher's description.
    Also available: Print – 2014
  • v. 2-, 2007- Springer
    Dhalla, Naranjan S.
  • 2014 Future Med
    Aronow, Wilbert S.
    Advances in dyslipidemia / Wilbert S. Aronow -- Atherosclerosis : dyslipidemia, inflammation and lipoapoptosis / Fleur M. van der Valk, Dominik M. Schulte, Willem J.M. Mulder & Erik S.G. Stroes -- Familial hypercholesterolemia : bridging and minding the gap in healthcare / Jing Pang, Alistair W. Vickery & Gerald F. Watts -- PCSK9 inhibition : drug development for low-density lipoprotein lowering / Geetha Mukerji, Rawand M. Abdin & Dominic S. Ng -- Novel therapies for dyslipidemia / Anthony S. Wierzbicki -- The highs and lows of high-density lipoprotein / Daniel Soffer & Marisa Schoen -- Do we need to treat patients with heart failure with statins? / Sadip Pant, Guru S. Gurumurthy, Abhishek Deshmukh & Jawahar L. Mehta -- Management of dyslipidemia in patients with nonalcoholic fatty liver disease / Konstantinos Tziomalos, Vasilios G. Athyros & Asterios Karagiannis -- Dyslipidemia : current management and the challenge of residual cardiovascular risk reduction / Aaron Rockoff, Vaijinath S. Kamanna & Moti L. Kashyap -- Index.
  • 2013 Springer
    Pletschke, Brett; Shukla, Pratyoosh.
    Improvement of thermostable enzyme with sugar metabolic activity by targeted mutagenesis -- Glycoside hydrolases for modification of glycosylation in polyphenolic antioxidants -- On the enzyme specificity for the synthesis of prebiotic galacto-oligosaccharides -- Microbial mannanases: properties and applications -- Enzyme Synergy for Enhanced Degradation of Lignocellulosic Waste -- Manganese peroxidase: molecular diversity, heterologous expression and applications -- Advance Techniques in Enzyme Research -- Regulatory motif identification in biological sequences: An overview of computational methodologies -- Structural, Molecular and Functional Aspects of Chitin deacetylase -- Role of enzymes and proteins in plant -- microbe interaction : A study of M. oryzae vs Rice -- Industrial enzyme applications in biorefineries for starchy materials.
  • 2012 Springer
    Bottoni, Patrizia; Giardina, B.; Scatena, Roberto.
    Also available: Print – 2012
  • 2015 Springer Protocols
    Reichelt, Senta.
    Affinity chromatography : a historical perspective -- Protein purification by amniosquarylium cyanine dye-affinity chromatography -- One-step purification of phosphinothricin acetyltransferase using reactive dye-affinity chromatography -- Antibody purification from human plasma by metal-chelated affinity membranes -- Specific recognition of supercoiled plasmid DNA by affinity chromatography using a synthetic aromatic ligand -- Cell affinity separations on microfluidic devices -- Aptamer-modified magnetic beads in affinity separation of proteins -- The Strep-tag system for one-step affinity purification of proteins from mammalian cell culture -- Robotic high-throughput purification of affinity-tagged recombinant proteins -- Macroporous silica particles derivatized for enhanced lectin affinity enrichment of glycoproteins -- Immobilized magnetic beads-based multi-target affinity selection coupled with HPLC-MS for screening active compounds from traditional Chinese medicine and natural products -- Mixed-bed affinity chromatography : principles and methods -- Preparation and characterization of fluorophenylboronic acid-functionalized affinity monolithic columns for the selective enrichment of cis-diol-containing biomolecules -- Introduction to macroporous cryogels -- Cryogels with affinity ligands as tools in protein purification -- Direct capture of His₆-tagged proteins using megaporous cryogels developed for metal-ion affinity chromatography -- Ni(II) chelated IDA functionalized poly(HEMA-GMA) cryogels for urease adsorption -- A novel chromatographic media : histidine-containing composite cryogels for HIgG separation from human serum -- Molecularity imprinted cryogels for human serum albumin depletion -- Interpenetrating polymer network composite cryogels with tailored porous morphology and sorption properties -- Analysis of drug-protein interactions by high-performance affinity chromatography : interactions of sulfonylurea drugs with normal and glycated human serum albumin -- Accurate protein-peptide titration experiments by nuclear magnetic resonance using low-volume samples -- Characterization of the binding strengths between boronic acids and cis-diol-containing biomolecules by affinity capillary electrophoresis -- Determination if the kinetic rate constant of cyclodextrin supramolecular systems by high-performance affinity chromatography -- Molecular modeling of the affinity chromatography of monoclonal antibodies.
  • 2006 Springer
    Horikoshi, Kōki.
  • 2008 Springer
    Traut, Thomas.
    Section 1. Overview of enzymes. Introduction to enzymes ; The limits for life define the limits for enzymes ; Enzyme kinetics ; Properties and evolution of allosteric enzymes ; Kinetics of allosteric enzymes -- Section 2. K-type enzymes. Hemoglobin ; Glycogen phosphorylase ; Phosphofructokinase ; Ribonucleotide reductase ; Hexokinase -- Section 3. V-type enzymes. Introduction to V-type enzymes ; G proteins ; Protein kinases.
  • 2012 Springer Protocols
    Fenton, Aron W.
    Binding techniques to study the allosteric energy cycle -- Kinetic trapping of a key hemoglobin intermediate -- Allosteric coupling between transition metal-binding sites in homooligomeric metal sensor proteins -- Studying the allosteric energy cycle by isothermal titration calorimetry -- Detecting "Silent" Allosteric coupling -- Using mutant cycle analysis to elucidate long-range functional coupling in allosteric receptors -- A review of methods used for identifying structural changes in a large protein complex -- Allosteric mechanisms of g protein-coupled receptor signaling: A structural perspective -- Dynamic light scattering to study allosteric regulation -- Dissecting the linkage between transcription factor self-assembly and site-specific DNA binding: The role of the analytical ultracentrifuge -- Fluorescence correlation spectroscopy and allostery: The case of GroEL -- The morpheein model of allostery: Evaluating proteins as potential morpheeins -- Combining NMR and molecular dynamics studies for insights into the allostery of small GTPase-protein interactions -- Hydrogen-deuterium exchange study of an allosteric energy cycle -- Ensemble properties of network rigidity reveal allosteric mechanisms -- An in vivo approach to isolating allosteric pathways using hybrid multimeric proteins -- Mutations in the GABA(A) receptor that mimic the allosteric ligand etomidate -- Allosteric regulation of human liver pyruvate kinase by peptides that mimic the phosphorylated/dephosphorylated N-terminus -- In silico-screening approaches for lead generation: Identification of novel allosteric modulators of human-erythrocyte pyruvate kinase -- Identification of allosteric-activating drug leads for human liver pyruvate kinase -- A critical evaluation of correlated mutation algorithms and coevolution within allosteric mechanisms -- The advantage of global fitting of data involving complex linked reactions -- Predicting binding sites by analyzing allosteric effects.
  • 2016 Springer
    Sandhaus, Robert A.; Wanner, Adam.
    Alpha-1- Antitrypsin and the Serpins -- Alpha-1 Antitrypsin: The protein -- Misfolding and Polymerisation of Alpha1-Antitrypsin ? Conformational Pathology and Therapeutic Targeting -- Managing the Adaptive Proteostatic Landscape: Restoring Resilience in Alpha-1 Antitrypsin Deficiency -- United States Targeted Detection Program for Alpha-1 Antitrypsin Deficiency -- Lung Disease of Alpha-1 Antitrypsin Deficiency -- Alpha-1-Antitrypsin Deficiency: Role in Health and Disease -- Alpha-1 Antitrypsin as a Therapeutic Agents for Conditions not Associated with Alpha-1 Antitrypsin Deficiency -- The Alpha-1 Constellation of Voluntary Health Organizations as a Paradigm for Confronting Other Rare Diseases.
  • 2012 Springer Protocols
    Alterman, Michail A.; Hunziker, Peter.
    Rapid LC-MS/MS profiling of protein amino acids and metabolically related compounds for large-scale assessment of metabolic phenotypes -- Combination of an AccQ. Tag-ultra performance liquid chromatographic method with tandem mass spectrometry for the analysis of amino acids -- Isotope dilution liquid chromatography-tandem mass spectrometry for quantitative amino acid analysis -- Analysis of underivatized amino acids: Zwitterionic hydrophilic interaction chromatography combined with triple quadrupole tandem mass spectrometry -- Amino acid analysis via LC-MS method after derivatization with quaternary phosphonium -- Amino acid analysis by hydrophilic interaction chromatography coupled with isotope dilution mass spectrometry -- A universal HPLC-MS method to determine the stereochemistry of common and unusual amino acids -- Amino acid analysis by capillary electrophoresis-mass spectrometry -- New advances in amino acid profiling by capillary electrophoresis-electrospray ionization-mass spectrometry -- Optimal conditions for the direct RP-HPLC determination of underivatized amino acids with online multiple detection -- Absolute quantitation of proteins by acid hydrolysis combined with amino acid detection by mass spectrometry -- Amino acid analysis by means of MALDI TOF mass spectrometry or MALDI TOF/TOF tandem mass spectrometry -- Heptafluorobutyl chloroformate-based sample preparation protocol for chiral and nonchiral amino acid analysis by gas chromatography -- The EZ:Faast family of amino acid analysis kits: Application of the GC-FID kit for rapid determination of plasma tryptophan and other amino acids -- Amino acid analysis in physiological samples by GC-MS with propyl chloroformate derivatization and iTRAQ-LC-MS/MS -- Automated analysis of primary amino acids in plasma by high-performance liquid chromatography -- RP-LC of phenylthiocarbamyl amino acid adducts in plasma acetonitrile extracts: Use of multiple internal standards and variable wavelength UV detection -- Quantification of underivatised amino acids on dry blood spot, plasma, and urine by HPLC-ESI-MS/MS -- Capillary electrophoresis of free amino acids in physiological fluids without derivatization employing direct or indirect absorbance detection -- Measurement of 3-nitro-tyrosine in human plasma and urine by gas chromatography-tandem mass spectrometry -- Analysis of hydroxyproline in collagen hydrolysates -- Innovative and rapid procedure for 4-hydroxyproline determination in meat-based foods -- Multiple reaction monitoring for the accurate quantification of amino acids: Using hydroxyproline to estimate collagen content -- Sequential injection chromatography for fluorimetric determination of intracellular amino acids in marine microalgae -- Direct analysis of underivatized amino acids in plant extracts by LC-MS-MS -- Wheat gluten amino acid analysis by high-performance anion-exchange chromatography with integrated pulsed amperometric detection -- Preparative HPLC separation of underivatized amino acids for isotopic analysis -- Quantification of amino acids in a single cell by microchip electrophoresis with chemiluminescence detection.
  • 2007 Springer
    Wendisch, Volker F.
  • 2013 CRCnetBASE
    Wu, Guoyao.
    This up-to-date book presents basic concepts and recent advances in amino acid biochemistry and nutrition, providing comprehensive coverage of developments over the past 45 years. It includes information on absorption, metabolism, and excretion of amino acids while addressing protein quality and requirements. The text discusses metabolic pathways of amino acids in relation to health and disease in various organisms, as well as species differences in metabolism of amino acids. The author also presents a critical analysis of amino acid supplementation to humans and animals.
  • 2012 Springer Protocols
    Calero, Miguel; Gasset, Maria; Sigurdsson, Einar M.
    Rapid generation of dityrosine cross-linked A-[beta] oligomers via cu-redox cycling / Adam P. Gunn, Blaine R. Roberts, and Ashley I. Bush -- Application of photochemical cross-linking to the study of oligomerization of amyloidogenic proteins / Dahabada H.J. Lopes [and others] -- Preparation of stable amyloid b-protein oligomers of defined assembly order / Clark Rosensweig [and others] -- Purification and fibrillation of full-length recombinant PrP / Natallia Makarava and Ilia V. Baskakov -- Featuring amyloids with fourier transform infrared and circular dichroism spectroscopies / Miguel Calero and María Gasset -- Quasielastic light scattering study of amyloid b-protein fibrillogenesis / Aleksey Lomakin and David B. Teplow -- Conformations of microtubule-associated protein tau mapped by fluorescence resonance energy transfer / Sadasivam Jeganathan [and others] -- Measuring the kinetics of amyloid fibril elongation using quartz crystal microbalances / Alexander K. Buell, Christopher M. Dobson, and Mark E. Welland -- X-ray fibre diffraction studies of amyloid fibrils / Kyle L. Morris and Louise C. Serpell -- Structural characterization of prefibrillar intermediates and amyloid fibrils by small-angle x-ray scattering / Annette Eva Langkilde and Bente Vestergaard -- Atomic force fluorescence microscopy in the characterization of amyloid fibril assembly and oligomeric intermediates / Valeriy Ostapchenko, Maria Gasset, and Ilia V. Baskakov -- Investigating fibrillar aggregates of tau protein by atomic force microscopy / Susanne Wegmann, Daniel J. Muller, and Eckhard Mandelkow -- Structural studies of amyloids by quenched hydrogen-deuterium exchange by NMR / Marçal Vilar, Lei Wang, and Roland Riek -- Cyclic amplification of prion protein misfolding / Marcelo A. Barria, Dennisse Gonzalez-Romero, and Claudio Soto -- Search for amyloid-binding proteins by affinity chromatography / Miguel Calero, Agueda Rostagno, and Jorge Ghiso -- Establishing the links between A[beta] aggregation and cytotoxicity in vitro using biophysical approaches / Asad Jan and Hilal A. Lashuel -- Preparation of cultured human vascular cells / Ingvar H. Olafsson, Dadi Th. Vilhjalmsson, and Finnbogi R. Thormodsson -- Murine cerebrovascular cells as a cell culture model for cerebral amyloid angiopathy : isolation of smooth muscle and endothelial cells from mouse brain / Sebastien A. Gauthier [and others] -- In vitro assays measuring protection by proteins such as cystatin C of primary cortical neuronal and smooth muscle cells / Sebastien A. Gauthier [and others] -- Study of neurotoxic intracellular calcium signalling triggered by amyloids / Carlos Villalobos [and others] -- Bacterial amyloids / Yizhou Zhou [and others] -- Study of amyloids using yeast / Reed B. Wickner [and others] -- Cell-to-cell transmission of [alpha]-synuclein aggregates / Seung-Jae Lee [and others] -- Subcutaneous adipose tissue biopsy for amyloid protein studies / Per Westermark -- Analysis of s100 oligomers and amyloids / Hugo M. Botelho, Günter Fritz, and Cláudio M. Gomes -- S100A8/A9 amyloidosis in the ageing prostate : relating ex vivo and in vitro studies / Anna L. Gharibyan, Dina Raveh, and Ludmilla A. Morozova-Roche -- Isolation of amyloid by solubilization in water / Dadi Th. Vilhjalmsson, Indiana E. Ingolfsdottir, and Finnbogi R. Thormodsson -- Histological staining of amyloid and pre-amyloid peptides and proteins in mouse tissue / Hameetha B. Rajamohamedsait and Einar M. Sigurdsson -- Pentameric luminescent-conjugated oligothiophene for optical imaging of in vitro-formed amyloid fibrils and protein aggregates in tissue sections / K. Peter R. Nilsson, Mikael Lindgren, and Per Hammarström -- In vivo magnetic resonance imaging of amyloid-[beta] plaques in mice / Youssef Zaim Wadghiri [and others] -- Mouse model for scrapie : inoculation, clinical scoring, and histopathological techniques / Michele A. Di Bari, Romolo Nonno, and Umberto Agrimi -- Biochemical isolation of insoluble tau in transgenic mouse models of tauopathies / Carl Julien, Alexis Bretteville, and Emmanuel Planel -- Tissue processing prior to analysis of alzheimer's disease associated proteins and metabolites, including A[beta] / Stephen D. Schmidt, Ralph A. Nixon, and Paul M. Mathews -- A-[beta] measurement by enzyme-linked immunosorbent assay / Stephen D. Schmidt [and others] -- Cognitive and sensorimotor tasks for assessing functional impairments in mouse models of Alzheimer's Disease and related disorders / Allal Boutajangout [and others].
  • 2012 Wiley
    Jiskoot, Wim; Mahler, Hanns-Christian.
    The critical need for robust assays for quantitation and characterization of aggregates of therapeutic proteins -- Separation based analytical methods for measuring protein aggregation -- Laser light scattering-based techniques used for the characterization of protein therapeutics -- Online detection methods and emerging techniques for soluble aggregates in protein pharmaceuticals -- Analytical methods to measure sub-visible particulates -- Detection of visible particles in parenteral products -- Characterization of aggregates and particles using emerging techniques -- Ultraviolet absorption spectroscopy -- Fluorescence spectroscopy to characterize protein aggregates and particles -- Infrared spectroscopy to characterize protein aggregates -- Raman microscopy for characterization of particles -- Microscopic methods for particle characterization in protein pharmaceuticals -- Comparison of methods for soluble aggregate detection and size characterization -- Protein purification and its relation to protein aggregation and particles -- Formulation development and its relation to protein aggregation and particles.
  • 2011 Springer
    Katoch, Rajan.
    Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.
  • 2015 ScienceDirect
    Cole, James L.
    Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software. This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals.
  • 2011 Springer
    Al-Rubeai, Mohamed.
    Engineered antibodies currently represent over 30 per cent of biopharmaceuticals in clinical trials and their total worldwide sales continue to increase significantly. The importance of antibody applications is reflected in their increasing clinical and industrial applications as well as in the progression of established and emerging production strategies. This volume provides detailed coverage of the generation, optimization, characterization, production and applications of antibody. It provides the necessary theoretical background and description of methods for the expression of antibody in.
  • 2013 Wiley
    Phoenix, David; Dennison, Sarah R.; Harris, Frederick.
    Antimicrobial peptides: their history, evolution, and functional promiscuity -- Cationic antimicrobial peptides -- Anionic antimicrobial peptides -- Graphical techniques to visualize the amphiphilic structures of antimicrobial peptides -- Models for the membrane interactions of antimicrobial peptides -- Selectivity and toxicity of oncolytic antimicrobial peptides.
  • 2009 Springer
    McCartney, Brooke M.; Näthke, Inke S.
    APC and [beta]-catenin degradation -- Nuclear APC -- APC in cell migration -- The APC-EB1 interaction -- The role of APC in mitosis and in chromosome instability -- Role of APC and its binding partners in regulating microtubules in mitosis -- The adenomatous polyposis coli tumor -- Suppressor and Wnt signaling in the regulation of apoptosis -- APC and its modifiers in colon cancer -- Tissue-specific tumour suppression by APC -- Extra-colonic manifestations of familial adenomatous polyposis coli.
    Also available: Print – 2009
  • 2015 Springer
    Anantharamaiah, G. M.; Goldberg, Dennis Ian.
  • 2013 Springer
    Kinter, Michael; Kinter, Caroline S.
    A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites. The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages. Most importantly, it is fundamentally quantitative with a wide dynamic range. The output of the analysis is a numerical result that can range over several orders of magnitude. Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures. Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment. This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins.
  • 2009 Springer
    Agre, Peter; Beitz, Eric.
  • 2011 Springer Protocols
    Duchaine, Thomas; Hobman, Tom C.
    Purification of native argonaute complexes from the fission yeast Schizosaccharomyces pombe / Shane M. Buker and Mohammad R. Motamedi -- Chromatin immunoprecipitation in fission yeast / Thomas A. Volpe and Jessica DeMaio -- Biochemical analyzes of endogenous argonaute complexes immunopurified with anti-argonaute monoclonal antibodies / Keita Miyoshi ... [et al.] -- Mapping of Ago2-GW182 functional interactions / Bing Yao ... [et al.] -- Continuous density gradients to study argonaute and GW182 complexes associated with the endocytic pathway / Derrick Gibbings -- In vitro RISC cleavage assay / Julia Stoehr and Gunter Meister -- Native gel analysis for RISC assembly / Tomoko Kawamata and Yukihide Tomari -- Purification and assembly of human argonaute, dicer, and TRBP complexes / Nabanita De and Ian J. MacRae -- Detection of human dicer and argonaute 2 catalytic activity / Marjorie P. Perron ... [et al.] -- Imaging the cellular dynamics of drosophila argonaute proteins / Jing Li ... [et al.] -- Live cell imaging of argonaute proteins in mammalian cells / Justin M. Pare, Joaquin Lopez-Orozco, and Tom C. Hobman -- Reporter-based assays for analyzing RNA interference in mammalian cells / Lydia V. McClure, Gil Ju Seo, and Christopher S. Sullivan -- Artificial tethering of argonaute proteins for studying their role in translational repression of target mRNAs / Stephanie Eckhardt ... [et al.] -- Efficient system for Let-7 microRNA and GW182 protein-mediated deadenylation in vitro / Marc R. Fabian, Yuri V. Svitkin, and Nahum Sonenberg -- Cell-free microRNA-mediated translation repression in Caenorhabditis elegans / Edlyn Wu and Thomas F. Duchaine -- Argonaute pull-down and RISC analysis using 2'-O -methylated oligonucleotides affinity matrices / Guillaume Jannot, Alejandro Vasquez-Rifo, and Martin J. Simard -- Cloning argonaute-associated small RNAs from Caenorhabditis elegans / Weifeng Gu ... [et al.] -- Immunoprecipitation of piRNPs and directional, next generation sequencing of piRNAs / Yohei Kirino ... [et al.] -- Generation of an inducible mouse ES cell lines deficient for argonaute proteins / Hong Su and Xiaozhong Wang -- Whole cell proteome regulation by microRNAs captured in a pulsed SILAC mass spectrometry approach / Olivia A. Ebner and Matthias Selbach.
  • 2014 Springer
    Gurevich, Vsevolod V.
    This volume describes our current understanding of the biological role of visual and non-visual arrestins in different cells and tissues, focusing on the mechanisms of arrestin-mediated regulation of GPCRs and non-receptor signaling proteins in health and disease. The book covers wide range of arrestin functions, emphasizing therapeutic potential of targeting arrestin interactions with individual partners.
  • 2005 Wiley
    Breslow, Ronald.
    Artificial enzymes / Ronald Breslow -- Vitamin B6 enzyme models / Lei Liu and Ronald Breslow -- Evolution of synthetic polymers with enzyme-like catalytic activities / Irving M. Klotz and Junghun Suh -- Mimicking enzymes with antibodies / Donald Hilvert -- Protein-based artificial enzymes / Ben Duckworth and Mark D. Distefano -- Artificial hydrolytic metalloenzymes / Jik Chin and Hae-Jo Kim -- Artificial restriction enzymes as tools for future molecular biology and biotechnology / Yoji Yamamoto and Makoto Komiyama.
  • 2014 Springer Protocols
    Ogawa, Atsushi.
  • 2010
    Dye, Natalie Anne; Shapiro, Lucy; Spudich, James A.; Straight, Aaron; Theriot, Julie A.
    This work focuses on the mechanism by which MreB contributes to the maintenance of cell shape in the gram-negative alpha-proteobacterium Caulobacter crescentus. The gene mreB encodes a protein that resembles actin, a eukaryotic cytoskeletal protein. Previously, it was shown that mreB is required to maintain a rod-like shape and localizes to a helical pattern near the cytoplasmic membrane. Here, we show that MreB is associated with regions of active growth in Caulobacter, as mutant strains that mislocalize MreB to the cell poles direct new growth at or near the poles. We present evidence to suggest that MreB contributes to the determination of proper length, width, and curvature through partially distinct mechanisms. The determination of proper width involves the essential proteins MreC and Pbp2, which are encoded in the mreB operon. While MreB and MreC are both required to position the cell wall transpeptidase Pbp2 along the lateral sidewalls and away from midcell, the two do not colocalize and each can maintain its localization in the absence of the other. When MreB is mislocalized to the poles, MreC and Pbp2 do not follow. These data argue against the idea that MreB provides a scaffold-like structure to localize enzymes that directly modify the cell wall. The determination of proper curvature, involves the intermediate filament-like protein, Crescentin. We identify a putative binding site on MreB for Crescentin or other curvature-mediating factors. We also show that the extent to which the subcellular localization of MreB changes over the cell cycle is correlated with cell size, indicating that MreB is involved in the coordination between elongation and division. In addition, we show that in vitro purified MreB spontaneously forms very stable polymers in the presence or absence of nucleotide. These polymers are globular or amorphous and only filamentous when placed on a highly positively charged surface of Poly-L-lysine. These in vitro data suggest that MreB is likely to be regulated at the disassembly step in the cell and that the cellular environment may influence the structure of MreB polymers. Lastly, we present biochemical evidence to support the existence of a disassembly factor in cytoplasmic Caulobacter extract. Together our data suggest that the maintenance of the crescent-rod cell shape in Caulobacter is the result of a complicated balance between MreB's dynamic subcellular localization, polymeric structure, and communication with cellular components.
  • 2015 Springer Protocols
    Buchanan, Susan K.; Noinaj, Nicholas.
    The [beta]-barrel assembly machinery complex / Denise L. Leyton, Matthew J. Belousoff, and Trevor Lithgow -- Yeast mitochondria as a model system to study the biogenesis of bacterial [beta]-barrel proteins / Thomas Ulrich [and three others] -- Experimental methods for studying the BAM complex in Neisseria meningitides / Martine P. Bos, Ria Tommassen-van Boxtel, and Jan Tommassen -- Heart modifiability of outer membrane proteins from gram-negative bacteria / Nicholas Noinaj, Adam J. Kuszak, and Susan K. Buchanan -- The role of a destabilized membrane of OMP insertion / Ashlee M. Plummer, Dennis Gessmann, and Karen G. Fleming -- Treponema pallidum in gel microdroplets: a method for topological analysis of BamA (TP0326) and localization of rare outer membrane proteins / Amit Luthra, Arvind Anand, and Justin D. Radolf -- Analyzing the role of periplasmic folding factors in the biogenesis of OMPs and members of the Type V secretion system / Gustavo Bodelón, Elvira Marín, and Luis Ángel Fernandez -- An in vitro assay for substrate translocation by FhaC in liposomes / Enguo Fan, Derrick Norell, and Matthias Müller -- Measuring cell-cell binding using flow-cytometry / Zachary C. Ruhe, Christopher S. Hayes, and David A. Low -- Methods to characterize folding and function of BamA cross-link mutants / Adam J. Kuszak, Nicholas Noinaj, and Susan K. Buchanan -- Small angle X-ray scattering (SAXS) characterization of the POTRA domains of BamA / Pamela Arden Doerner and Marcelo Carlos Sousa -- Assessing the outer membrane insertion and folding of multimeric transmembrane [beta]-barrel proteins / Jack C. Leo, Philipp Oberhettinger, and Dirk Linke -- The expression, purification, and structure determination of BamA from E. coli / Dongchun Ni and Yihua Huang -- Expression and purification of the individual Bam components BamB-E / Suraaj Aulakh, Kelly H. Kim, and Mark Paetzel -- Structure determination of the BAM complex accessory lipoproteins BamB-E / Kornelius Zeth -- An in vitro assay for outer membrane protein assembly by the BAM complex / Giselle Roman-Hernandez and Harris D. Bernstein -- Identification of BamC on the surface of E. coli / Chaille T. Webb and Trevor Lithgow -- Construction and characterization of an E. coli bamD depletion strain / Dante R. Ricci -- Expression, purification, and screening of BamE, a component of the BAM complex, for structural characterization / Mark Jeeves, Pooja Sridhar, and Timothy J. Knowles -- Purification and bicelle crystallization for structure determination of the E. coli outer membrane protein TamA / Fabian Gruss, Sebastian Hiller, and Timm Maier -- Strategies for the analysis of Bam recognition motifs in outer membrane proteins / Nagarajan Paramasivam and Dirk Linke -- Summary and future directions / Nicholas Noinaj and Susan K. Buchanan
  • 2006 Springer
    Holtzhauer, M.
  • 1999 NCBI Bookshelf
    Agranoff, Bernard W.; Siegel, George J.
    Also available: Print – 1999
  • 2010 Springer
    Hetz, Claudio.
    Homeostatic functions of BCL-2 proteins beyond apoptosis / Nika N. Danial, Alfredo Gimenez-Cassina, and Daniel Tondera -- Alternative functions of the BCL-2 protein family at the endoplasmic reticulum / Diego Rojas-Rivera ... [et al.] -- BH3-only proteins and their effects on cancer / Thanh-Trang Vo and Anthony Letai -- Endoplasmic reticulum stress and BCL-2 family members / Ross T. Weston and Hamsa Puthalakath -- Targeting survival pathways in lymphoma / Luca Paoluzzi and Owen A. O'Connor -- The interplay between BCL-2 family proteins and mitochondrial morphology in the regulation of apoptosis / Maria Eugenia Soriano and Luca Scorrano -- Noncanonical functions of BCL-2 proteins in the nervous system / Heather M. Lamb and J. Marie Hardwick -- Mitochondria on guard : role of mitochondrial fusion and fission in the regulation of apoptosis / Mariusz Karbowski.
    Also available: Print – 2010
  • 2012 AccessMedicine
    Janson, Lee W.; Tischler, Marc E.
    Section I. The Basic Molecules of Life -- 1. Amino acids and proteins -- 2. Carbohydrates -- 3. Lipids -- 4. Nucleosides, nucleotides -- Section II. Functional biochemistry -- 5. Enzymes and amino acid metabolism -- 6. Carbohydrate metabolism -- 7. Lipid metabolism -- 8. Membranes -- 9. DNA/RNA function and protein synthesis -- Section III. Applied biochemistry -- 10. Metabolism and vitamins/minerals / Maria L. Valencik and Cynthia C. Mastick -- 11. The Digestive system / Kshama Jaiswal -- 12. Muscles and cell motility / Darren Campbell -- 13. Connective tissue and bone / Jacques Kerr -- 14. Blood / Matthew Porteus -- 15. The Immune system / Eric L. Greidinger, Leonard M. Miller -- 16. The Cardiovascular system / Ralph Shohet, John A. Burns -- 17. The respiratory system / Howard Huang -- 18. The Urinary system / Armando J. Lorenzo -- 19. The Nervous system / Kathryn Beck-Yoo -- 20. The Reproductive system / Catrina Bubier -- Section IV. Appendices -- Appendix I. Biochemical Basis of Diseases -- Appendix II. Biochemical Methods -- Appendix III. Organic Chemistry Primer.
  • 2007 Springer
    Huopalahti, Rainer.
  • 2010 CRCnetBASE
    Owusu-Apenten, R. K.
    "Presenting data from human studies and trials, along with recent research findings, this work summarizes the applications, and benefits of bioactive peptides used to mitigate major metabolic derangements arising from chronic illnesses and resulting in unwanted weight loss. Recent studies show bioactive peptides to enhance the body's antioxidant status, antisepsis capacity, immune function, anti-inflammatory capacity, mineral absorption, and appetite. This book covers general principles, such as host response, quality factors, protein economics, and muscle loss. It includes case studies on ageing, AIDS, COPD, diabetes, inflammatory bowel disease, kidney failure, and tuberculosis."--Provided by publisher.
  • 2015 Springer
    Demeule, Barthélemy; Rosenberg, Amy.
  • 2013 Springer
    Dhalla, Naranjan S.; Mehta, Jawahar.
    Angiogenesis plays a key role in human physiology and pathophysiology. While necessary for tissue growth and development, uncontrolled angiogenesis plays a role in the progression of certain tumors as well as atherosclerosis. Lack of angiogenesis may be the basis of myocardial ischemia and stroke. Knowledge on the mechanisms of angiogenesis is growing very rapidly, and may lead to important drugs for therapy of a variety of clinical disorders. Experts from around the world have contributed a vast array of data on different aspects of angiogenesis in this volume edited by Professors Mehta and Dhalla. This information will be of immense help to basic scientists, clinicians and those involved in drug development.
  • 2015 Springer
    Chakrabarti, Abhijit; Surolia, Avadhesha.
    Consists of critical reviews and original research papers from the 2014 International Symposium on the "Biochemical Role of Eukaryotic Cell Surface Macromolecules". Topics covered include: · neurochemical and biochemical analysis of cell surface glycoconjugates · membrane skeletal organization · GPCRs and other receptors · biophysical approaches to study membrane interactions · glycoconjugate metabolism · dysregulation · molecular mechanisms involved in cell-cell and cell-matrix interaction · glycans in infectious and neurological diseases · cancer and glycosyltransferases as drug targets.
    Also available: Print – 2015
  • 2012 Springer
    Sudhakaran, Perumana R.; Surolia, Avadhesha.
    Glycomics: An Overview of the Complex Glycocode / Garima Gupta and Avadhesha Surolia -- Structural Glycomic Approaches to Molecular Recognition Events on Cell Surfaces / Koichi Kato -- ISCSM2011 Chondroitin Sulfate E-type Structure at Tumor Cell Surface Is Involved in Experimental Metastasis / Kazuyuki Sugahara and Shuji Mizumoto -- Chondroitin Sulfate-Specific Novel Hydrolase in Human / Shuhei Yamada -- Identification of Endothelial Cell Surface Carbohydrate-Binding Receptors by Carbohydrate Ligand Mimicry Peptides / Michiko N. Fukuda -- Lysosomal Enzyme Sorting Receptors - Where Did They First Appear in the Animal Kingdom? / Suryanarayanaraju Vegiraju, Suresh Koduru and Siva Kumar Nadimpalli -- Endoplasmic Reticulum-Targeted Bcl-2 Inhibitable Mitochondrial Fragmentation Initiates ER Stress-Induced Cell Death / B. C. Bhavya, Deepa Indira, Mahendra Seervi, Jeena Joseph and Praveen K. Sobhan, et al. -- Interactions Between Caveolin-1 and Sphingolipids, and Their Functional Relevance / Sandro Sonnino, Simona Prioni, Vanna Chigorno and Alessandro Prinetti -- Cell Membrane Repair Pathway Involves Sensing of Dynamics of Caveolae and Caspase-1 / Saumya S. Srivastava and M. V. Krishnasastry -- Angiogenic Response of Endothelial Cells to Fibronectin / V. B. S. Kumar, R. I. Viji, M. S. Kiran and Perumana R. Sudhakaran -- Lactosylceramide Synthase as a Therapeutic Target to Mitigate Multiple Human Diseases in Animal Models / Subroto Chatterjee and Nezar Alsaeedi -- Advanced FRET Methodologies: Protein-Lipid Selectivity Detection and Quantification / Fábio Fernandes, Manuel Prieto and Luís M. S. Loura -- Mechanism of GPCR-Directed Autoantibodies in Diseases / Hamiyet Unal, Rajaganapathi Jagannathan and Sadashiva S. Karnik -- Role of Membrane Cholesterol in Leishmanial Infection / Amitabha Chattopadhyay and Md. Jafurulla -- How Intact Is the Basement Membrane? Role of MMPs / S. Asha Nair, Sankar Jagadeeshan, Ramachandran Indu, Perumana R. Sudhakaran and M. R. Pillai -- Apoptosis of Breast Cancer Cells: Modulation of Genes for Glycoconjugate Biosynthesis and Targeted Drug Delivery / Subhash Basu, Rui Ma, Joseph R. Moskal, Manju Basu and Sipra Banerjee -- Altered Expression of Sialidases in Human Cancer / Taeko Miyagi, K. Takahashi, S. Moriya, K. Hata and K. Yamamoto, et al. -- Poly-ADP-Ribosylation of Vascular Endothelial Growth Factor and Its Implications on Angiogenesis / S. Binu, S. J. Soumya, V. B. S. Kumar and Perumana R. Sudhakaran -- Sphingolipid-Binding Domain in the Serotonin1A Receptor / Amitabha Chattopadhyay, Yamuna Devi Paila, Sandeep Shrivastava, Shrish Tiwari and Pushpendra Singh, et al. -- Withanolide D, Carrying the Baton of Indian Rasayana Herb as a Lead Candidate of Antileukemic Agent in Modern Medicine / Susmita Mondal, Saptarshi Roy, Rita Maity, Asish Mallick and Rajender Sangwan, et al. -- Engineered Glucose to Generate a Spectroscopic Probe for Studying Carbohydrate Biology / Ashish Tripathi, Vibha Singh, K. G. Aishwarya, Gopala Krishna Aradhyam and Srinivas Hotha -- Changes in Sialic Acid Content of Jelly Coat in Pesticide-Exposed Frog Eggs and Their Influence on Fertilization / H. P. Gurushankara, S. V. Krishnamurthy and V. Vasudev.
    Also available: Print – 2012
  • 2006 Wiley
    Alberty, Robert A.
    Accompanying CD-ROM contains the full text, as well as macros that help scientists and engineers solve their particular problems.
    Also available: Print – 2006
  • 2007
    Wilson, Golder.
    Status: Not Checked OutLane Catalog Record
  • 2013
    Wilson, Golder; Tonk, Vijay S.
    Status: Not Checked OutLane Catalog Record
  • 2010
    Wilson, Golder.
    Status: Not Checked OutLane Catalog Record
    High Yield Facts -- DNA Structure, Replication and Repair -- Gene Expression and Regulation -- Acid-base equilibria, amino acids, and protein structure -- Protein Structure/Function -- Carbohydrate Metabolism -- Bioenergetics and Energy Metabolism -- Lipid, Amino Acid and Nucleotide Metabolism -- Vitamins and Minerals -- Hormones and Integrated Metabolism -- Inheritance Mechanisms/Risk Calculations -- Genetic and Biochemical Diagnosis.
  • 2007 CRCnetBASE
    Lundblad, Roger L.
    Abbreviations and acronyms -- Glossary of terms useful in biochemistry and molecular biology and related disciplines -- Chemicals commonly used in biochemistry and molecular biology and their properties -- A listing of log P values, water solubility, and molecular weight for some selected chemicals -- Protease inhibitors and protease inhibitor cocktails -- List of buffers -- Organic name reactions useful in biochemistry and molecular biology.
  • 2015 ScienceDirect
    Komoda, Tsugikazu; Matsunaga, Toshiyuki.
  • 2007
    Pelley, John W.; Goljan, Edward F.
  • 2006 Springer
    Cheema, Sukhinder Kaur.
  • 2008 ScienceDirect
    Vance, Dennis E.; Vance, Jean E.
    Functional roles of lipids in membranes / William Dowhan, Mikhail Bogdanov, and Eugenia Mileykovskaya -- Lipid modifications of proteins / Anant K. Menon -- Fatty acid and phospholipid metabolism in prokaryotes / Charles O. Rock -- Lipid metabolism in plants / Katherine M. Schmid and John B. Ohlrogge -- Oxidation of fatty acids in eukaryotes / Horst Schulz -- Fatty acid synthesis in eukaryotes / Hei Sook Sul and Stuart Smith -- Fatty acid desaturation and chain elongation in mammals / Makoto Miyazaki and James N. Ntambi -- Phospholipid biosynthesis in eukaryotes / Dennis E. Vance and Jean E. Vance -- Ether-linked lipids and their bioactive species / Thomas M. McIntyre, Fred Snyder, and Gopal K. Marathe -- Lipid metabolism in adipose tissue / Ann V. Hertzel ... [et al.] -- Phospholipases / David C. Wilton -- The eicosanoids : cyclooxygenase, lipoxygenase, and epoxygenase pathways / William L. Smith and Robert C. Murphy -- Sphingolipids / Alfred H. Merrill, Jr. -- Cholesterol biosynthesis / Laura Liscum -- Metabolism and function of bile acids / Luis B. Agellon -- Lipid assembly into cell membranes / Dennis R. Voelker -- Lipoprotein structure / Ana Jonas and Michael C. Phillips -- Assembly and secretion of triacylglycerol-rich lipoproteins / Jean E. Vance and Khosrow Adeli -- Dynamics of lipoprotein transport in the circulatory system / Christopher J. Fielding and Phoebe E. Fielding -- Lipoprotein receptors / Wolfgang J. Schneider -- Lipids and atherosclerosis / Ira Tabas.
    Also available: Print – 2008
  • 2014 Springer
    Asson-Batres, Mary Ann; Rochette-Egly, Cécile.
    A role for vitamin A in living organisms has been known throughout human history. In the last 100 years, the biochemical nature of vitamin A and its active derivative, retinoic acid, its physiological impact on growth processes and the essential details of its mechanism of action have been revealed by investigations carried out by researchers using vertebrate and more recently invertebrate models to study a multiplicity of processes and conditions, encompassing embryogenesis, postnatal development to old age. A wealth of intercellular interactions, intracellular signaling systems and molecular mechanisms have been described and the overall conclusion is that retinoic acid is essential for life. This book series, with chapters authored by experts in every aspect of this complex field, unifies the knowledge base and mechanisms currently known in detailed, engaging, well-illustrated, focused chapters that synthesize information for each specific area. In view of the recent explosion in this field, it is timely to publish a contemporary, comprehensive, book series recapitulating the most exciting developments in the field and covering fundamental research in molecular mechanisms of vitamin A action, its role in physiology, development and continued well-being and the potential of vitamin A derivatives and synthetic mimetics to serve as therapeutic treatments for cancers and other debilitating human diseases. VOLUME I: Here, we present the first volume of a multi-volume series on Retinoic Acid Signaling that will cover all aspects of this broad and diverse field. One aim of Volume I is to present a compilation of topics related to the biochemistry of nuclear retinoic acid receptors, from their architecture when bound to DNA and associated with their coregulators to their ability to regulate target gene transcription. A second aim is to provide insight into recent advances that have been made in identifying novel targets and non-genomic effects of retinoic acid. Volume I is divided into ten chapters contributed by prominent experts in their respective fields. Each chapter starts with the history of the area of research. Then, the key findings that contributed to development of the field are described, followed by a detailed look at key findings and progress that are being made in current, ongoing research. Each chapter is concluded with a discussion of the relevance of the research and a perspective on missing pieces and lingering gaps that the author recommends will be important in defining future directions in vitamin A research.
  • 2005 Wiley
    Krauss, Gerhard.
    The regulation of gene expression -- The regulation of enzyme activity -- Structure and function of signal pathways -- Signaling by nuclear receptors -- G protein-coupled signal transmission pathways -- Intracellular messenger substance : second messengers -- Ser/Thr-specific protein kinases and protein phosphates -- Signal transmission via transmembrane receptors with tyrosine-specific protein kinase activity -- Signal transmission via ras proteins -- Intracellular signal transduction : the protein cascades of the MAP kinase pathways -- Membrane receptors with associated tyrosine kinase activity -- Other receptor classes -- Regulation of the cell cycle -- Malfunction of signaling pathways and tumorigenesis : oncogenes and tumor suppressor genes -- Apoptosis.
  • 2011 ClinicalKey
    Pelley, John W.; Goljan, Edward F.
    Carbohydrates, lipids, and amino acids : metabolic fuels and biosynthetic precursors -- Proteins and enzymes -- Membrane biochemistry and signal transduction -- Nutrition -- Generation of energy from dietary fuels -- Carbohydrate metabolism -- Lipid metabolism -- Nitrogen metabolism -- Integration of metabolism -- Nucleotide synthesis and metabolism -- Organization, synthesis, and repair of DNA -- Gene expression -- DNA technology -- Common laboratory values.
    Also available: Print – 2010
  • 2002 NCBI Bookshelf
    Berg, Jeremy M.; Stryer, Lubert; Tymoczko, John L.
    Also available: Print – 2002
  • 2008
    Swanson, Todd A.; Glucksman, Marc J.; Kim, Sandra.
    Status: Not Checked OutLane Catalog Record
  • v. 1-2, 2001-03.
    Metzler, David E.
    Status: Not Checked OutLane Catalog Record
  • 2013 ScienceDirect
    Hermanson, Greg T.
    Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. *Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab *Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates *Features full color illustrations *Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented.
  • 2011 Springer Protocols
    Mark, Sonny S.
    Site-specific labeling of proteins for single-molecule FRET measurements using genetically encoded ketone functionalities / Edward A. Lemke -- Enzymatically catalyzed conjugation of a biodegradable polymer to proteins and small molecules using microbial transglutaminase / Ahmed Besheer [and others] -- Synthesis of drug/dye-incorporated polymer-protein hybrids / Sukanta Dolai [and others] -- Dye/DNA conjugates as multiple labels for antibodies in sensitive fluorescence immunoassays / Qin Zhang, Shengchao Zhu, and Liang-Hong Guo -- Chemoselective modification of viral proteins bearing metabolically introduced "clickable" amino acids and sugars / Partha S. Banerjee and Isaac S. Carrico -- Preparation of peptide and other biomolecular conjugates through chemoselective ligations / Mathieu Galibert [and others] -- New fluorescent substrates of microbial transglutaminase and its application to peptide tag-directed covalent protein labeling / Noriho Kamiya and Hiroki Abe -- Covalent conjugation of poly(ethylene glycol) to proteins and peptides : strategies and methods / Anna Mero [and others] -- Extending the scope of site-specific cysteine bioconjugation by appending a prelabeled cysteine tag to proteins using protein trans-splicing / Tulika Dhar, Thomas Kurpiers, and Henning D. Mootz -- Polyethylenimine bioconjugates for imaging and DNA delivery in vivo / Andrea Masotti and Francesco Pampaloni -- Synthesis of a glycomimetic oligonucleotide conjugate by 1,3-dipolar cycloaddition / Gwladys Pourceau [and others] -- Site-specific DNA labeling by staudinger ligation / Samuel H. Weisbrod, Anna Baccaro, and Andreas Marx -- Improved cellular uptake of antisense peptide nucleic acids by conjugation to a cell-penetrating peptide and a lipid domain / Takehiko Shiraishi and Peter E. Nielsen -- Synthesis of oligonucleotide-peptide conjugates for biomedical and technological applications / Anna Avino [and others] -- Amphiphilic DNA block copolymers : nucleic acid-polymer hybrid materials for diagnostics and biomedicine / Jan Zimmermann [and others] -- Chemically selective liposome surface glyco-functionalization / Hailong Zhang, Yong Ma, and Xue-Long Sun -- Bioconjugation using mutant glycosyltransferases for the site-specific labeling of biomolecules with sugars carrying chemical handles / Boopathy Ramakrishnan [and others] -- Lipid-core-peptide system for self-adjuvanting synthetic vaccine delivery / Mariusz Skwarczynski and Istvan Toth -- Coupling carbohydrates to proteins for glycoconjugate vaccine development using a pentenoyl group as a convenient linker / Qianli Wang and Zhongwu Guo -- Conjugation of LPS-derived oligosaccharides to proteins using oxime chemistry / Joanna Kubler-Kielb -- Site-specific chemical modification of a glycoprotein fragment expressed in yeast / Junpeng Xiao and Thomas J. Tolbert -- On-resin convergent synthesis of a glycopeptide from HIV gp120 containing a high mannose type N-linked oligosaccharide / Rui Chen and Thomas J. Tolbert -- Design and synthesis of novel functional lipid-based bioconjugates for drug delivery and other applications / Rupa R. Sawant and Vladimir P. Torchilin -- Chemical functionalization and bioconjugation strategies for atomic force microscope cantilevers / Magnus Bergkvist and Nathaniel C. Cady -- Chemoselective protein and peptide immobilization on biosensor surfaces / Edith H.M. Lempens, Brett A. Helms, and Maarten Merkx -- Fabrication of dynamic self-assembled monolayers for cell migration and adhesion studies / Nathan P. Westcott and Muhammad N. Yousaf -- DNA detection using functionalized conducting polymers / Jadranka Travas-Sejdic [and others] -- Preparation and dynamic patterning of supported lipid membranes mimicking cell membranes / Stefan Kaufmann, Karthik Kumar, and Erik Reimhult -- Enzyme immobilization on reactive polymer films / Ana L. Cordeiro [and others] -- Characterization of protein-membrane binding interactions via a microplate assay employing whole Liposome Immobilization / Matthew D. Smith and Michael D. Best -- Bioconjugated phospholipid polymer biointerface with nanometer-scaled structure for highly sensitive immunoassays / Kazuki Nishizawa, Madoka Takai, and Kazuhiko Ishihara -- Purification, functionalization, and bioconjugation of carbon nanotubes / John H.T. Luong [and others] -- Functional integration of membrane proteins with nanotube and nanowire transistor devices / Aleksandr Noy [and others] -- Single-step conjugation of antibodies to quantum dots for labeling cell surface receptors in mammalian cells / Gopal Iyer, Jianmin Xu, and Shimon Weiss -- Practical strategy for constructing nanodrugs using carbon nanotubes as carriers / Wei Wu and Xiqun Jiang -- Design and synthesis of biofunctionalized metallic/magnetic nanomaterials / Eun-Kyung Lim [and others].
  • 2005 Springer
    Mullins, Chris.
    Theory of organelle biogenesis : a historical perspective / Barbara M. Mullock and J. Paul Luzio -- Protein coats as mediators of intracellular sorting and organelle biogenesis / Chris Mullins -- The role of proteins and lipids in organelle biogenesis in the secretory pathway / Thomas F.J. Martin -- Endoplasmic reticulum biogenesis : proliferation and differentiation / Erik Snapp -- The golgi apparatus : structure, function and cellular dynamics / Nihal Altan-Bonnet and Jennifer Lippincott-Schwartz -- Lysosome biogenesis and dynamics / Diane McVey Ward, Shelly L. Shiflett and Jerry Kaplan -- Nucleogenesis / Sui Huang -- Mitochondrial biogenesis / Danielle Leuenberger, Sean P. Curran and Carla M. Koehler -- The biogenesis and cell biology of peroxisomes in human health and disease / Stanley R. Terlecky and Paul A. Walton.
  • 2013 Springer
    Wang, Xiangdong.
    Clinical Bioinformatics in Human Proteomics Research / Duojiao Wu, Haihao Li -- Proteomics Defines Protein Interaction Network of Signaling Pathways / Shitao Li -- Protein Function Microarrays: Design, Use and Bioinformatic Analysis in Cancer Biomarker Discovery and Quantitation / Jessica Duarte, Jean-Michel Serufuri, Nicola Mulder -- Proteomics and Cancer Research / Elena Lopez Villar -- Toward Development of Novel Peptide-Based Cancer Therapeutics: Computational Design and Experimental Evaluation / Elena Pirogova, Taghrid Istivan -- Advances in Proteomic Methods / Xianyin Lai -- Clinical and Biomedical Mass Spectrometry: New Frontiers in Drug Developments and Diagnosis / Ákos Végvári, Melinda Rezeli -- Disease Biomarkers: Modelling MR Spectroscopy and Clinical Applications / Luis Martí-Bonmati -- Processing of Mass Spectrometry Data in Clinical Applications / Dario Di Silvestre, Pietro Brunetti, Pier Luigi Mauri -- Bioinformatics Approach for Finding Target Protein in Infectious Disease / Hemant Ritturaj Kushwaha, Indira Ghosh -- Identification of Network Biomarkers for Cancer Diagnosis / Jiajia Chen, Luonan Chen -- Software Development for Quantitative Proteomics Using Stable Isotope Labeling / Xin Huang, Shi-Jian Ding -- Clinical Translation of Protein Biomarkers Integrated with Bioinformatics / Xu Yang, Juanjuan Zhou, Chaoqin Du -- Proteomic Approaches for Urine Biomarker Discovery in Bladder Cancer / Ming-Hui Yang, Yu-Chang Tyan -- Antibody Microarrays and Multiplexing / Jerry Zhou, Larissa Belov, Nicola Armstrong -- Proteomics in Anaesthesia and Intensive Care Medicine / Ornella Piazza, Giuseppe De Benedictis.
  • 2002 Wiley
    Roat-Malone, Rosette M.
    ch. 1. Inorganic chemistry essentials -- ch. 2. Biochemistry fundamentals -- ch. 3. Instrumental and computer-based methods -- ch. 4. Iron containing oxygen carriers and their synthetic models -- ch. 5. Copper Enzymes -- ch. 6. The Enzyme nitrogenase -- ch. 7. Metals in medicine.
  • 2007 Wiley
    Roat-Malone, Rosette M.
    ch. 1. Inorganic chemistry essentials -- ch. 2. Biochemistry fundamentals -- ch. 3. Instrumental methods -- ch. 4. Computer hardware, software, and computational chemistry methods -- ch. 5 Group I and II metals in biological systems : homeostasis and group I biomolecules -- ch. 6. Group I and II metals in biological systems : group II -- ch. 7. Iron-containing proteins and enzymes.
  • 2012 Wiley
    Brown, Nathan.
  • 2007 Springer
    Bonucci, E.
  • 2014 ScienceDirect
    Ishii, Ken J.; Tang, Choon Kit.
    Biological DNA Sensor defines the meaning of DNA sensing pathways and demonstrates the importance of the innate immune responses induced by double stranded DNA (dsDNA) through its influencing functions in disease pathology and immune activity of adjuvants for vaccines. Though discussed in specific subsections of existing books, dsDNA and its immunogenic properties has never received the complete treatment given in this book. Biological DNA Sensor approaches the impact of dsDNA's immunogenicity on disease and vaccinology holistically. It paints a complete and concise picture on the topic so you can understand this area of study and make more informed choices for your respective research needs. Chapters are authored by researchers who are renowned for their research focus, ensuring that this book provides the most complete views on the topics. Multi-authored by a distinguished panel of world-class expertsIdeal source of information for those wanting to learn about DNA sensingProvides in-depth explanations of DNA sensing pathways and the innate immune system, bridging the gap between them
  • 2005 ScienceDirect
    Burlingame, A. L.
    Also available: Print – 2005
  • 2006 Springer
    Biswas, B. B.; Majumder, A. Lahiri.
  • 2011 ScienceDirect
    Bird, Phillip I.; Whisstock, James C.
    Also available: Print – 2011
  • 2012 Springer
    Rőszer, Tamás.
    This book fills in a gap in the NO literature. Recent progress in the field of NO-biology shows that NO is generated within distinct cell compartments, including specific plasma membrane regions, mitochondria, chloroplasts, peroxisomes, the Golgi-complex and intracellular membrane systems. NO synthesis plays specific roles in these compartments and, in turn, cell organelles also control intracellular NO levels. This monograph focuses on the roles played by the subcellular NO-signaling microdomains in the prokaryote-, fungus-, plant- and animal cells and shows how NO behaves as an intracellular signal in distinct cellular environments. This monograph also provides a summary of our knowledge on how NO synthesis came through evolution to be associated with organelles and subcellular compartments. Promotes the novel ideas that some functions of NO and its associations with subcellular units have been conserved during the evolution of the cell. A special chapter is dedicated to the biomedical relevance of subcellular NO synthesis, and this chapter also discusses the evidence that altered compartmentalization of NO-producing enzymes causes disease.
  • 2010 Springer
    Shahid, Mohammed.
  • 2014 ScienceDirect
    Lyubchenko, Yuri L.; Uversky, Vladimir N.
    Bio-Nanoimaging: Protein Misfolding & Aggregation provides a unique introduction to both novel and established nanoimaging techniques for visualization and characterization of misfolded and aggregated protein species. The book is divided into three sections covering: - Nanotechnology and nanoimaging technology, including cryoelectron microscopy of beta(2)-microglobulin, studying amyloidogensis by FRET; and scanning tunneling microscopy of protein deposits - Polymorphisms of protein misfolded and aggregated species, including fibrillar polymorphism, amyloid-like protofibrils, and insulin olig.
  • 2013 Springer
    Dinman, Jonathan D.
    When quantum mechanics was first proposed a century ago, nobody could have anticipated how deeply it would affect our lives. Today, we are connected and powered through devices whose existence is predicated on the basic principles of this strange physics. Not even the biological sciences have escaped its reach. As scientists query the deepest mysteries of the living world, the physical scales probed and the types of questions asked are increasingly blurring the lines between biology and physics. The hybrid field of biophysics represents the new frontier of the 21st century. The ribosome has been at the heart of three Nobel Prizes. Understanding its essential nature and how it interacts with other proteins and nucleic acids to control protein synthesis has been one of the central foundations in our understanding of the biology at the molecular level. With the advent of atomic scale structures, methods to visualize and separate individual molecules, and the computational power to model the complex interactions of over a million atoms at once, our understanding of how gene expression is controlled at the level of protein translation is now deeply ensconced in the biophysical realm. This book provides a premier resource to a wide audience, whether it be the general reader seeking a broad view of the field, a clinician interested in the role of protein translation in human disease, the bench researcher looking for state-of-the-art technologies, or computational scientists involved in cutting edge molecular modeling.
  • pt. A-B, 2009. ScienceDirect
    pt. B, 2009 ScienceDirect
    Herschlag, Daniel.
    Also available: Print – pt. A-B, 2009.
  • v. 1, 1958.
    Edsall, John T.; Wyman, Jeffries.
    Status: Not Checked OutLane Catalog Record
  • 2012 Knovel
    Cooper, Alan.
    1. Biological molecules -- 2. Spectroscopy -- 3. Mass spectrometry -- 4. Hydrodynamics -- 5. Thermodynamics and interactions -- 6. Kinetics -- 7. Chromatography and electrophoresis -- 8. Imaging -- 9. Single molecules.
  • 2011 Springer
    Buxbaum, Engelbert.
  • 2013 CRCnetBASE
    Lundblad, Roger L.
    "Discussing the role of plasma proteins in current biotechnology, this book describes the protein composition of human plasma, the fractionation of plasma to obtain therapeutic proteins, and the analysis of these products. It delineates the path from plasma products to recombinant products, and highlights products from albumin, intravenous immunoglobins, and coagulation. It offers a comprehensive review of current techniques for the analysis of proteins including electrophoresis, chromatography, spectrophotometry, and mass spectrometry as well as updates not published since 1975"--Provided by publisher.
  • pt. A-D, 2009-11. ScienceDirect
    pt. B, 2009 ScienceDirect
    pt. C, 2011 ScienceDirect
    pt. D, 2011 ScienceDirect
    Ackers, Gary K.; Holt, Jo M.; Johnson, Michael L.
  • 2010 Springer
    Cancro, M. P.
  • 2001 Karger
    Hills, Andrew P.; Jürimäe, T.
    Also available: Print – 2001
  • 2013 Springer
    Agrò, Felice Eugenio.
    The administration of intravenous fluids is one of the most common and important therapeutic practices in the treatment of surgical, medical and critically ill patients. The international literature accordingly contains a vast number of works on fluid management, yet there is still confusion as to the best options in the various situations encountered in clinical practice.The purpose of this volume is to help the decision-making process by comparing different solution properties describing their indications, mechanisms of action and side-effects according to physiologic body water distribution, electrolytic and acid-base balance, and to clarify which products available on the market represent the best choice in different circumstances. The book opens by discussing in detail the concepts central to a sound understanding of abnormalities in fluid and electrolyte homeostasis and the effect of intravenous fluid administration. In the second part of the monograph, these concepts are used to explain the advantages and disadvantages of solutions available on the market in different clinical settings. Body Fluid Management: From Physiology to Therapy will serve as an invaluable decision-making guide, including for those who are not experts in the subject.
  • 2007 Springer
    Hung, Mien-chie; Yu, Dihua.
    Overview of resistance to systemic therapy in patients with breast cancer / Ana Maria Gonzalez-Angulo, Flavia Morales-Vasquez and Gabriel N. Hortobagyi -- Roles of multidrug resistance genes in breast cancer chemoresistance / M. Tien Kuo -- Therapy-induced apoptosis in primary tumors / David J. McConkey -- Cell cycle deregulation in breast cancer: insurmountable chemoresistance or achilles' heel? / Laura Lambert and Khandan Keyomarsi -- p53, BRCA1 and breast cancer chemoresistance / Kimberly A. Scata and Wafik S. El-Deiry -- Integrin-mediated adhesion: tipping the balance between chemosensitivity and chemoresistance / Mary M. Zutter -- Insulin-like growth factors and breast cancer therapy / Xianke Zeng and Douglas Yee -- EGF receptor in breast cancer chemoresistance / Robert B. Dickson and T.B. Deb -- Molecular mechanisms of ErbB2-mediated breast cancer chemoresistance / Ming Tan and Dihua Yu -- Estrogen receptors in resistance to hormone therapy / Matthew H. Herynk and Suzanne A.W. Fuqua -- Novel approaches for chemosensitization of breast cancer cells: the E1A story / Yong Liao, Dihua Yu and Mien-Chie Hung.
    Also available: Print – 2007
  • 2010 Springer
    Dickerson, Ian M.; Hay, Debbie L.
  • 2007 ScienceDirect
    Krebs, J.; Michalak, Marek.
    Also available: Print – 2007
  • 2010
    Gomez-Ospina, Natalia; Clandinin, Thomas R.; Crabtree, Gerald R.; Dolmetsch, Ricardo E.; Meyer, Tobias.
    Abstract : Voltage-gated calcium channels are an important route of calcium entry into cells and are essential for converting electrical activity into biochemical events. In neurons these channels are vital for synaptic vesicle release and have been implicated in almost every activity-dependent process including survival, dendritic arborization, synaptic plasticity, and gene expression. One of the ways in which these channels regulate cellular behavior is by regulating gene expression but the mechanisms that link calcium channels to the transcription machinery are not well understood. In this thesis I show that a C-terminal fragment of CaV1.2, an L-type voltage-gated calcium channel, translocates to the nucleus and regulates transcription. I show that this calcium channel associated transcription regulator (CCAT), binds to a nuclear protein, associates with an endogenous promoter, and regulates the expression of a variety of endogenous genes that are important for the function of neurons and muscle cells. The nuclear localization of CCAT is regulated by changes in intracellular calcium on a time scale of minutes, suggesting that CCAT integrates information about the electrical activity of the cell. Together these findings reveal an entirely unexpected function for a well-characterized calcium channel. This works also addresses the question of how CCAT is generated. I show that CCAT is not released from proteolysis of full-length Cav1.2 channel but is generated from an mRNA that is transcribed from the 3' end of the Cav1.2 gene (CACNA1C). Consistent with this, I find that CCAT expression is independent of full-length channel protein and that exon 46 of the CACNA1C gene contains a promoter whose transcriptional activity is required for the expression of CCAT. Activity at this promoter, and consequently CCAT expression, is regulated spatially and temporally in the brain having highest expression during embryonic stages and in regions of the brain rich in inhibitory neurons. Analysis of 5' transcriptional starts from CACNA1C and Cap Analysis of Gene Expression (CAGE) tags from genome-wide studies show at least two mRNAs one of which encodes CCAT in vivo and a second transcript that is predicted to encode a membrane bound CCAT containing a voltage sensor. These findings reveal an unexpected mechanism by which CCAT is generated in neurons and provide a unique example by which two proteins with distinct biologic functions can be derived from a single gene. Such transcriptional phenomena may be at play in many other genes throughout the genome and has far reaching implications for prediction of gene products and interpretation of phenotypes in gene mutations and knockout studies.
  • 2010 Springer Protocols
    Petersen, O. H.; Verkhratskiĭ, A. N.
    Principles of the CA2+ homeostatic/signalling system / A. Verkhratsky and O.H. Petersen -- CA2+ recordings: Hardware and software (from microscopes to cameras) / E.C. Toescu and J. Graham -- CA2+ imaging: Principles of analysis and enhancement / F. Mammano and M. Bortolozzi -- Bioluminescent CA2+ indicators / L. Fedrizzi and M. Brini -- Monitoring calcium levels with genetically encoded indicators / O. Garaschuk and O. Griesbeck -- Intracellular calcium-sensitive microelectrodes / R.C. Thomas -- CA2+ caging and uncaging / S.H. Kim and M.K. Park -- CA2+ imaging of intracellular organelles: Endoplasmic reticulum / R. Blum, O.H. Petersen and A. Verkhratsky -- CA2+ imaging of intracellular organelles: Mitochondria / L. Núñez [and others] -- CA2+ imaging of dendrites and spines / K. Holthoff -- In vivo CA2+ imaging of the living brain using multi-cell bolus loading technique / G. Eichhoff [and others] -- CA2+ imaging of glia / C. Lohr and J.W. Deitmer.
  • 2012 Springer
    Islam, Md. Shahidul.
    "This volume ... [explores] topics ranging from the fundamental aspects of calcium signaling to its varied clinical implications. It presents comprehensive discussion of cutting-edge research alongside detailed analysis of critical issues, at the same time as setting out testable hypotheses that point the way to future scientific endeavors. The contributions feature material on theoretical and methodological topics as well as related subjects including mathematical modeling and simulations. They examine calcium signaling in a host of contexts, from mammalian cells to oocytes, zebrafish and plants. With much of interest to newcomers to the field as well as seasoned experts, this new publication is both wide-ranging and authoritative." --Excerpt from publisher's description.
    Also available: Print – 2012
  • 2014 Springer
    Wang, Yong-Xiao.
    Ryanodine and Inositol Trisphosphate Receptors/Ca2+ Release Channels in Airway Smooth Muscle Cells / Lin Mei, Yun-Min Zheng, and Yong-Xiao Wang -- Kv7 (KCNQ) Potassium Channels and L-type Calcium Channels in the Regulation of Airway Diameter / Kenneth L. Byron, Lioubov I. Brueggemann, Priyanka P. Kakad, and Jennifer M. Haick -- Transient Receptor Potential and Orai Channels in Airway Smooth Muscle Cells / Jun-Hua Xiao, Yong-Xiao Wang, and Yun-Min Zheng -- Large-conductance calcium-activated potassium channels / Hiroaki Kume -- Calcium-activated Chloride Channels / George Gallos and Charles W. Emala Sr. -- Local Calcium Signaling in Airway Smooth Muscle Cells / Quing-Hua Liu, Carlo Savoia, Yong-Xiao Wang -- Regulation of Airway Smooth Muscle Contraction by Ca2+ Signaling: Physiology Revealed by Microscopy Studies of Lung Slices / Michael J. Sanderson -- Temporal Aspects of Ca2+ Signaling in Airway Myocytes / Etienne Roux -- Mechanisms Underlying Ca2+ Store Refilling in Airway Smooth Muscle / Charlotte K. Billington, Ian P. Hall, and Carl P. Nelson -- Novel Mechanisms in Ca2+-homeostasis and Internal Store Refilling of Airway Smooth Muscle / Luke J. Janssen -- The Role of Mitochondria in Calcium Regulation in Airway Smooth Muscle / Philippe Delmotte, Li Jia, and Gary C. Sieck -- Role of Caveolae in the Airway / Christina M. Pabelick, Brij B. Singh, and Y.S. Prakash -- CD38 : Cyclic ADP-ribose-mediated Calcium Signaling in Airway Myocytes /Deepak A. Deshpande, Alonso Guedes, Mythili Dileepan, Timothy F. Walseth, and Mathur S. Kannan -- The Pathways and Signaling Cross-talk with Oxidant in Calcium Influx in Airway Smooth Muscle Cells / Lei Cai and Quinghua Hu -- Role of RhoA/Rho-kinase and Calcium Sensitivity in Airway Smooth Muscle Functions / Satoru Ito -- Role of Integrins in the Regulation of Calcium Signaling / Thai Tran and Chun Ming Teoh -- Sex Steroid Signaling in the Airway / Y.S. Prakash, Venkatachalem Sathish, and Elizabeth A. Townsend -- Regulation of Contractility in Immature Airway Smooth Muscle / Y.S. Prakash, Christina M. Pabelick, and Richard J. Martin -- Mathematical Modeling of Calcium Dynamics in Airway Smooth Muscle Cells / James Sneyd, Pengxing Cao, Xiahui Tan, and Michael J. Sanderson -- Effects of Inflammatory Cytokines on Ca2+ Homeostasis in Airway Smooth Muscle Cells / Hisako Matsumoto -- Ca2+ Signaling and P2 Receptors in Airway Smooth Muscle / Luis M. Montaño, Edgar Flores-Soto, and Carlos Barajas-López -- Calcium Signaling in Airway Smooth Muscle Remodeling / Tengyao Song, Yun-min Zheng, and Yong-Xiao Wang -- Regulation of Intracellular Calcium by Bitter Taste Receptors on Airway Smooth Muscle / Deepak A. Deshpande and Stephen B. Liggett -- Modulation of Airway Smooth Muscle Contractile Function by TNFa and IL-13 and Airway Hyper-responsiveness in Asthma / Yassine Amrani -- Airway Smooth Muscle Malfunction in COPD / Yunchao Su.
  • 2012 Springer Protocols
    Lambert, David G.; Rainbow, Richard D.
    Fluorescent measurement of [Ca2+]c : basic practical considerations / Alec W.M. Simpson -- Measurement of [Ca2+]i in whole cell suspensions using fura-2 / Anish Patel [and others] -- Confocal microscopy : theory and applications for cellular signaling / Stephen C. Tovey [and others] -- Ratiometric Ca2+ measurements using the FlexStation® scanning fluorometer / Ian C.B. Marshall, Izzy Boyfield, and Shaun McNulty -- Measuring Ca2+ changes in multiwell format using the fluorometric imaging plate reader (FLIPR®) / Ian C.B. Marshall, Davina E. Owen, and Shaun McNulty -- Ratiometric [Ca2+]i measurements in adherent cell-lines using the NOVOstar microplate reader / Benjamin D. Hunt and David G. Lambert -- Whole-cell patch-clamp recording of voltage-sensitive Ca2+ channel currents in single cells : heterologous expression systems and neurones / Jon Brown [and others] -- Combined calcium fluorescence recording with ionic currents in contractile cells / Richard D. Rainbow -- Measurement of phospholipase C by monitoring inositol phosphates using [3H]inositol labeling protocols in permeabilized cell / Alison Skippen, Philip Swigart, and Shamshad Cockcroft -- Single-cell imaging techniques for the real-time detection of IP3 in live cells / Carl P. Nelson -- Measurement of inositol(1,4,5)trisphosphate using a stereospecific radioreceptor mass assay / Darren Smart -- Measurement of [Ca2+]i in smooth muscle strips using front-surface fluorimetry / Hideo Kanaide and Katsuya Hirano -- Calcium measurements from whole heart using rhod-2 / Bum-Rak Choi -- Measurement of changes in endothelial and smooth muscle Ca2+ in pressurized arteries / Kim A. Dora and Michael A. Hill -- Single cell and subcellular measurements of intracellular Ca2+ concentration / John G. McCarron [and others] -- Simultaneous analysis of intracellular pH and Ca2+ from cell populations / Raul Martinez-Zaguilan [and others] -- Measurements of Ca2+ concentration with recombinant targeted luminescent probes / Denis Ottolini, Tito Calì, and Marisa Brini -- Chimeric G proteins in fluorimetric calcium assays : experience with opioid receptors / Valeria Camarda and Girolamo Calo -- Compartmentalizing genetically encoded calcium sensors / David A. Williams, Mastura Monif, and Kate L. Richardson -- Measurement of cytosolic-free Ca2+ in plant tissue / Martin R. McAinsh and Carl K.-Y. Ng -- Measurement of Ca2+-ATPase activity (in PMCA and SERCA1) / Danuta Kosk-Kosicka.
  • v. 1-2, 2002. Springer Protocols
    v. 2, 2002 Springer Protocols
    Vogel, Hans J.
    v. 1. Reviews and case studies -- v. 2. Methods and techniques.
  • 2013 Springer Protocols
    Heizmann, Claus W.
    Measurement of intracellular Ca2+ concentration in single cells using ratiometric calcium dyes -- Divers models of divalent cation interaction to calcium-binding proteins: Techniques and anthology -- Probing Ca2+-binding capability of viral proteins with the EF-hand motif by grafting approach -- New aspects of calmodulin-calmodulin binding domains recognition -- Purification and characterization of the human cysteine-rich S100A3 protein and its pseudo citrullinated forms expressed in insect cells -- X-ray structural analysis of S100 proteins -- Purification and stable isotope labeling of the calcium- and integrin-binding protein 1 for structural and functional NMR studies -- Analysis of calcium-induced conformational changes in calcium-binding allergens and quantitative determination of their IgE binding properties -- Longistatin, an EF-hand Ca2+-binding protein from vector tick: Identification, purification, and characterization -- Super-resolution microscopy of the neuronal calcium-binding proteins calneuron-1 and caldendrin -- NMR studies of the interaction of calmodulin with iq motif peptides -- Biochemical and immunological detection of physical interactions between penta-ef-hand protein ALG-2 and its binding partners -- Measuring binding of S100 proteins to RAGE by surface plasmon resonance -- Phage display selection of peptides that target calcium-binding proteins -- RAGE-mediated cell signaling -- RAGE splicing variants in mammals -- Screening for novel calcium-binding proteins that regulate cardiac hypertrophy: CIB1 as an example -- In vivo screening of S100B inhibitors for melanoma therapy -- Arrayed primer extension microarray for the analysis of genes associated with congenital stationary night blindness -- Nesfatin-1: Its role in the diagnosis and treatment of obesity and some psychiatric disorders -- Human cell line authentication: The critical first step in any project using human cell lines -- Detection of Ca2+-binding S100 proteins in human saliva by HPLC-ESI-MS -- Clinical use of the calcium-binding S100B protein -- Sensible use of high-sensitivity troponin assays -- S100a1 gene therapy in small and large animals.
  • 2016 ScienceDirect
    Feig, Andrew L.
  • 2008 Springer
    Daoud, Sayed S.
    Proteomics technologies -- Cell signaling proteomics -- Tumor proteomics -- Bioinformatics and regulatory aspects of proteomics.
  • 2013 Springer Protocols
    Maccari, Francesca; Volpi, Nicola.
    Recent advances in capillary electrophoresis-based proteomic techniques for biomarker discovery / Chenchen Wang, Xueping Fang, and Cheng S. Lee -- Capillary electrophoresis-mass spectrometry of carbohydrates / Joseph Zaia -- Approaches to enhancing the sensitivity of carbohydrate separations in capillary electrophoresis / M.C. Breadmore -- Determination of monosaccharides derivatized with 2-aminobenzoic acid by capillary electrophoresis / Mitsuru Abo, Li-ping He, Kae Sato and Akira Okubo -- Determination of mono-, di-, and oligosaccharides by capillary electrophoresis with capacitively coupled contactless conductivity detection / Claudimir Lucio do Lago, Thiago Nogueira, Lucas Blanes and Renata Mayumi Saito -- Separation of chitooligosaccharides in acidic solution by capillary electrophoresis / Toshiaki Hattori, Nobuhiro Anraku, and Ryo Kato -- Capillary electrophoresis for the analysis of glycosaminoglycan-derived disaccharides / Yuqing Chang, Bo Yang, Amanda Weyers and Robert J. Linhardt -- High-throughput capillary electrophoresis-mass spectrometry : from analysis of amino acids to analysis of protein complexes / Mehdi Moini -- Separation of amino acids by capillary electrophoresis with light-emitting diode-induced fluorescence in the presence of electroosmotic flow / Ming-Mu Hsieh and Po-Ling Chang -- Quantification of arginine and dimethylated arginines in human plasma by field-amplified sample injection capillary electrophoresis UV detection / Angelo Zinellu, Salvatore Sotgia, Luca Deianna and Ciriaco Carru -- Capillary electrophoresis-mass spectrometry for peptide analysis : target-based approaches and proteomics/peptidomics strategies / Carolina Simó, Alejandro Cifuentes, and Václav Kašička -- High resolution proteome/peptidome analysis of body fluids by capillary electrophoresis coupled with MS / Amaya Albalat, Vasiliki Bitsika, Petra Zurbig, Justyna Siwy and William Mullen -- Contribution of CE to the analysis of protein or peptide biomarkers / Kiarach Mesbah, Romain Verpillot, Franc̦ois de l'Escaille, Jean Bernard Falmagne and Myriam Taverna -- Highly charged polyelectrolyte coatings to prevent adsorption during protein and peptide analysis in capillary electrophoresis / Reine Nehmé and Catherine Perrin -- Capillary electrophoresis with laser-induced fluorescence detection of proteins from two types of complex sample matrices : food and biological fluids / Raul Garrido-Medina, Angel Puerta, Cristina Pelaez-Lorenzo, Zuly Rivera-Monroy, Andras Guttman, Jose Carlos Diez-Masa and Mercedes de Frutos -- Automated capillary electrophoresis in the screening for hemoglobinopathies / Frédéric Cotton, Fleur Wolff, and Béatrice Gulbis -- Protein fingerprinting of Staphylococcus aureus by capillary electrophoresis with on-capillary derivatization and laser-induced fluorescence detection / Cristina Pelaez-Lorenzo, Maria Teresa Veledo, Ramon Gonzales, Mercedes de Frutos and Jose Carlos Diez-Masa -- Capillary electrophoresis of seed storage proteins : the separation and identification of microheterogeneous rice glutelin subunits / Tomoyuki Katsube-Tanaka -- Evaluating amyloid beta (A[beta]) 1-40 degradation by capillary electrophoresis / Benjamin J. Alper and Walter K. Schmidt -- Capillary electrophoresis for protein profiling of the dimorphic, pathogenic fungus, penicillium marneffei / Julie M. Chandler, Heather R. Trenary, Gary R. Walker and Chester R. Cooper -- Capillary electrophoretic enzyme assays / Gerhard K.E. Scriba, Hans Abromeit, Martina Hense and Yi Fan -- A methodology for detection and quantification of esterase activity / Ana L. Simplício, Ana S. Coroadinha, John F. Gilmer and Joana Lamego -- Enzyme inhibitor screening by CE with and on-column immobilized enzyme microreactor created by an ionic binding technique / Chao Liu, Qianqian Zhang, and Jingwu Kang -- Fluorescent lipids as probes for sphingosine kinase activity by capillary electrophoresis / Philip M. Yangyuoru, Latanya Hammonds-Odie and Simon M. Mwongela -- Assessment of DNA damage by micellar electrokinetic chromatography / Paolo Fattorini, Giorgio Marrubini, Pierangela Grignani, Solange Sorc̦aburu-Cigliero and Carlo Previderé.
  • 2014 Springer
    Abdel-Salam, Omar M. E.
    This volume provides an up-to-date account of the achievements pertaining to the application of capsaicin and capsaicin-like molecules in the therapy of various human ailments such as pain, non-allergic rhinitis, obesity, tumors, and gastrointestinal, dermatologic, and urologic disorders. It discusses the basic functions of the capsaicin receptor (TRPV1), its mechanisms of action, and its role in physiological and pathological processes. The text focuses on the most recent progress in the use of capsaicin and capsaicin-like molecules as a therapeutic agent and highlights potential pharmaceutical implications of further TRPV1 research. The chapters are written by noted experts in their fields of endeavor. This book offers both clinicians and researchers valuable resource and reference material on the subject that will stimulate future research.
  • 2012 Springer Protocols
    Chevolot, Yann.
    Recent advances and future challenges in glycan microarray technology / José L. de Paz and Peter H. Seeberger -- Chemical synthesis of carbohydrates and their surface immobilization : a brief introduction / Daniel B. Werz -- General consideration on sialic acid chemistry / Hongzhi Cao and Xi Chen -- Synthesis of azido-functionalized carbohydrates for the design of glycoconjugates / Sami Cecioni [and others] -- Polypyrrole-oligosaccharide microarray for the measurement of biomolecular interactions by surface plasmon resonance imaging / Julia Bartoli, André Roget, and Thierry Livache -- Glycosylated self-assembled monolayers for arrays and surface analysis / Fang Cheng and Daniel M. Ratner -- Carbohydrate microarrays for enzymatic reactions and quantification of binding affinities for glycan-protein interactions / Myung-Ryul Lee, Sungjin Park, and Injae Shin -- Neoglycolipid-based oligosaccharide microarray system : preparation of NGLs and their noncovalent immobilization on nitrocellulose-coated glass slides for microarray analyses / Yan Liu [and others] -- Preparation of a mannose-6-phosphate glycan microarray through fluorescent derivatization, phosphorylation, and immobilization of natural high-mannose N-glycans and application in ligand identification of P-type lectins / Xuezheng Song [and others] -- Production of fluorous-based microarrays with uncharged carbohydrates / Sahana K. Nagapayya and Nicola L.B. Pohl -- General procedure for the synthesis of neoglycoproteins and immobilization on epoxide-modified glass slides / Yalong Zhang and Jeffrey C. Gildersleeve -- Immobilization of polyacrylamide-based glycoconjugates on solid phase in immunosorbent assays / Oxana E. Galanina [and others] -- Surface plasmon resonance imaging analysis of protein binding to a sialoside-based carbohydrate microarray / Matthew J. Linman [and others] -- Glycoarray by DNA-directed immobilization / Franc̦ois Morvan [and others] -- Fabrication of carbohydrate surfaces by using non-derivatised oligosaccharides / Jonathan Popplewell [and others] -- Polysaccharide microarrays : application to the identification of heparan sulphate mimetics / Julien Dheur [and others] -- Carbohydrate antigen microarrays / Denong Wang -- Probing virus-glycan interactions using glycan microarrays / Jamie Heimburg-Molinaro [and others] -- MALDI-ToF MS analysis of glycosyltransferase activities on gold surface arrays / Nicolas Laurent [and others] -- Microarray technology using glycans extracted from natural sources for serum antibody fluorescent detection / Emanuela Lonardi [and others] -- Studying modification of aminoglycoside antibiotics by resistance-causing enzymes via microarray / Matthew D. Disney -- Microarray method for the rapid detection of glycosaminoglycan-protein interactions / Claude J. Rogers and Linda C. Hsieh-Wilson -- Neoglycolipid-based "designer" oligosaccharide microarrays to define [beta]-glucan ligands for Dectin-1 / Angelina S. Palma [and others] -- Measurement of antibodies to pneumococcal polysaccharides with luminex xMAP microsphere-based liquid arrays / Jerry W. Pickering and Harry R. Hill -- Carbohydrate microarrays in 96-well polystyrene microtiter plates / Jean-Philippe Ebran, Nabil Dendane, and Oleg Melnyk -- Photoimmobilization of saccharides / Gregory T. Carroll and Denong Wang -- Microwave-assisted method for fabrication of carbohydrate cluster microarrays on 3-dimensional hydrazide-dendrimer substrate / Xichun Zhou, Jian Zhang, and Denong Wang -- Combinatorial glycoarray / Simon Rinaldi, Kathryn M. Brennan, and Hugh J. Willison.
  • 2003 Wiley
    Wong, Chi-Huey.
    ch. 1. Synthetic methodologies / Chikako Saotome, Osamu Kanie -- ch. 2. Complex carbohydrate synthesis / Makoto Kiso, Hideharu Ishida, Hiromune Ando -- ch. 3. The chemistry of sialic acid / Geert-Jan Boons, Alexei V. Demchenko -- ch. 4. Solid-phase oligosaccharide synthesis / Peter H. Seeberger -- ch. 5. Solution and polymer-supported synthesis of carbohydrates / Shin-Ichiro Nishimura -- ch. 6. Enzymatic synthesis of oligosaccharides / Jianbo Zhang, Jun Shao, Prezemk Kowal, Peng George Wang -- ch. 7. Glycopeptides and glycoproteins : synthetic chemistry and biology / Oliver Seitz -- ch. 8. Synthesis of complex carbohydrates : Everninomicin 13,384-1 / K. C. Nicolaou, Helen J. Mitchell, Scott A. Snyder -- ch. 9. Chemical synthesis of asparagine-linked glycoprotein oligosaccharides : recent examples / Yukishige Ito, Ichiro Matsuo -- ch. 10. Chemistry and biochemistry of asparagine-linked protein glycosylation / Barbara Imperiali, Vincent W.-F. Tai -- ch. 11. Conformational analysis of C-glycosides and related compounds : programming conformational profiles of C- and O-glycosides / Peter G. Goekjian, Alexander Wei, Yoshito Kishi -- ch. 12. Synthetic lipid A antagonists for sepsis treatment / William J. Christ, Lynn D. Hawkins, Michael D. Lewis, Yoshito Kishi -- ch. 13. Polysialic acid vaccines / Harold J. Jennings -- ch. 14. Synthetic carbohydrate-based vaccines / Stacy J. Keding, Samuel J. Danishefsky -- ch. 15. Chemistry, biochemistry, and pharmaceutical potentials of glycosaminoglycans and related saccharides / Tasneem Islam, Robert J. Linhardt -- ch. 16. A new generation of antithrombotics based on synthetic oligosaccharides / Maurice Petitou, Jean-Marc Herbert -- ch. 17. Sequencing of oligosaccharides and glycoproteins / Stuart M. Haslam, Kay-Hooi Khoo, Anne Dell -- ch. 18. Preparation of heterocyclic 2-deoxystreptamine aminoglycoside analogues and characterization of their interaction with RNAs by use of electrospray ionization mass spectrometry / Richard H. Griffey, Steven A. Hofstadler, Eric E. Swayze -- ch. 19. Glycosylation analysis of a recombinant P-selectin antagonist by high-pH anion-exchange chromatography with pulsed electrochemical detection (HPAEC/PED) / Mark R. Hardy, Richard J. Cornell -- ch. 20. Analytical techniques for the characterization and sequencing of glycosaminoglycans / Ram Sasisekharan, Zachary Shriver, Mallik Sundaram, Ganesh Venkataraman -- ch. 21. Thermodynamic models of the multivalency effect / Pavel I. Kitov, David R. Bundle -- ch. 22. Synthetic multivalent carbohydrate ligands as effectors or inhibitors of biological processes / Laura L. Kiessling, Jason K. Pontrello, Michael C. Schuster -- ch. 23. Glycosyltransferase inhibitors / Karl-Heinz Jung, Richard R. Schmidt -- ch. 24. RNA-aminoglycoside interactions / Haim Weizman, Yitzhak Tor -- ch. 25. Glycosylated natural products / Jon S. Thorson, Thomas Vogt -- ch. 26. Novel enzymatic mechanisms in the biosynthesis of unusual sugars / Alexander Wong, Xuemei He, Hung-Wen Liu -- ch. 27. Neoglycolipids : identification of functional carbohydrate epitopes / Ten Feizi, Alexander M. Lawson, Wengang Chai -- ch. 28. A preamble to aglycone reconstruction for membrane-presented glycolipid mimics / Murugesapillai Mylvaganam, Clifford A. Lingwood -- ch. 29. Small molecule inhibitors of the sulfotransferases / Dawn E. Verdugo, Lars C. Pedersen, Carolyn R. Bertozzi -- ch. 30. Carbohydrate-based treatment of cancer metastasis / Reiji Kannagi -- ch. 31. N-acetylneuraminic acid derivatives and mimetics as anti-influenza agents / Robin Thomson, Mark Von Itzstein -- ch. 32. Modified and modifying sugars as a new tool for the development of therapeutic agents : the biochemically engineered N-acyl side chain of sialic acid : biological implications and possible uses in medicine / Rüdiger Horstkorte, Oliver T. Keppler, Werner Reutter -- ch.33 . Modified and modifying sugars as a new tool for the development of therapeutic agents : glycosidated phospholipids as a new type of antiproliferative agents / Kerstin Danker, Annette Fischer, Werner Reutter -- ch. 34. Glycoside primers and inhibitors of glycosylation / Jillian R. Brown, Mark M. Fuster, Jeffrey D. Esko -- ch. 35. Carbohydrate-based drug discovery in the battle against bacterial infections : new opportunities arising from programmable one-pot oligosaccharide synthesis / Thomas K. Ritter, Prof. Dr. Chi-Huey Wong.
  • 2012 CRCnetBASE
    Grunwald, Peter.
    1. Basics in carbohydrate chemistry / Heinrich Hühnerfuss -- 2. Glycoconjugates : a brief overview / Peter Grunwald -- 3. Oligosaccharides and glycoconjugates in recognition processes / Thisbe K. Lindhorst -- 4. Glycoside hydrolases / Motomitsu Kitaoka -- 5. Disaccharide phosphorylases : mechanistic diversity and application in the glycosciences / Christiane Luley-Goedl and Bernd Nidetzky -- 6. Enzymatic and chemoenzymatic synthesis of nucleotide sugars : novel enzymes, novel substrates, novel products, and novel routes / Leonie Engels and Lothar Elling -- 7. Iteratively acting glycosyltransferases / Marta Luzhetska and Andreas Bechthold -- 8. Bacterial glycosyltransferases involved in molecular mimicry of mammalian glycans / Warren Wakarchuk -- 9. Sulfotransferases and sulfatases : sulfate modification of carbohydrates / Eli Chapman and Sarah R. Hanson -- 10. Glycosylation in health and disease / Peter Grunwald -- 11. Sialic acid derivatives, analogues, and mimetics as biological probes and inhibitors of sialic acid recognizing proteins / Joe Tiralongo and Thomas Haselhorst -- 12. Enzymes of the carbohydrate metabolism and catabolism for chemoenzymatic syntheses of complex oligosaccharides / Julian Thimm and Joachim Thiem -- 13. From gene to product : tailor-made oligosaccharides and polysaccharides by enzyme and substrate engineering / Jürgen Seibel -- 14. Synthesis and modification of carbohydrates via metabolic pathway engineering in microorganisms / Xian-wei Liu ... [et al.] -- 15. Metabolic pathway engineering for hyaluronic acid production / Esteban Marcellin, Wendy Y. Chen, and Lars K. Nielsen -- 16. Microbial rhamnolipids / Markus M. Müller ... [et al.] -- 17. Chitin-converting enzymes / Karin Moss ... [et al.] -- 18. Linear and cyclic oligosaccharides / Hajime Taniguchi -- 19. Fungal degradation of plant oligo- and polysaccharides / Ronald P. de Vries ... [et al.] -- 20. Bacterial strategies for plant cell degradation and their genomic information / Yutaka Tamaru and Roy H. Doi -- 21. Design of efficient multienzymatic reactions for cellulosic biomass processing / Anne S. Meyer.
  • 2014 Springer
    Frost, Susan C.; McKenna, Robert.
    The study of carbonic anhydrase has spanned multiple generations of scientists. Carbonic anhydrase was first discovered in 1932 by Meldrum and Roughton. Inhibition by sulfanilamide was shown in 1940 by Mann and Keilin. Even Hans Krebs contributed to early studies with a paper in 1948 showing the relationship of 25 different sulfonamides to CA inhibition. It was he who pointed out the importance of both the charged and uncharged character of these compounds for physiological experiments. The field of study that focuses on carbonic anhydrase (CA) has exploded in recent years with the identification.
  • 2013 Springer
    Dhalla, Naranjan S.; Oštá̕dal, Bohuslav.
    The processes of adaptation and maladaptation play an important role in the pathogeny of serious cardiovascular diseases, such as hypertension, valvular diseases, congenital heart disease, myocardial infarction and different cardiomyopathies as well as during adaptation to exercise and high altitude hypoxia. This volume incorporates the rapidly developing basic and clinically relevant information on adaptive mechanisms, thereby contributing to the better understanding of possible prevention and therapy of life-threatening cardiovascular diseases. The first section of this volume focuses on developmental aspects of cardiac adaptation, including chapters on comparative and molecular aspects of cardiac development, prenatal and postnatal developments, coronary vascular development, and ontogenetic adaptation to hypoxia, as well as cardiac and arterial adaptation during aging. The second section is devoted to cardiac adaptations to overload on the heart, centered around the mechanisms of cardiac hypertrophy due to pressure overload, volume overload, exercise, gender difference, high altitude, and different pathological situations. The third section of this volume highlights the roles of sympathetic nervous system with respect to [alpha]-adrenoceptor and [beta]-adrenoceptor mechanisms in the development of cardiac hypertrophy. Cardiac Adaptations will be of great value to cardiovascular investigators, who will find this book highly useful in their cardiovascular studies for finding solutions in diverse pathological conditions; it will also appeal to students, fellows, scientists, and clinicians interested in cardiovascular abnormalities.
  • 2015 Springer
    Dixon, Ian M. C.; Wigle, Jeffrey.
    Cardiac fibrosis and heart failure-- cause or effect? -- Fibroblast activation in the infarcted myocardium -- Mechanical and matrix regulation of valvular fibrosis -- Bone marrow-derived progenitor cells, micro-RNA, and fibrosis -- The stressful life of cardiac myofibroblasts -- Pathogenic origins of fibrosis in the hypertensive heart disease that accompnaies aldosternoism -- Embryological origin of valve progenitor cells -- Diverse cellular origin of valve progenitor cells -- Diverse cellular origins of cardiac fibroblasts -- Non-canonical regulation of TGF-β1 signaling: a role for Ski/Sno and YAP/TAZ -- Molecular mechanisms of smooth muscle and fibroblast phenotype conversions in the failing heart -- Current and future strategies for the diagnosis and treatment of cardiac fibrosis -- Remodelling of the cardiac extracellular matrix: role of collagen degradation and accumulation in pathogenesis of heart failure -- Matrix metalloproteinase 9 (MMP-9) -- Collagen processing and its role in fibrosis -- Mechanisms of cardiac fibrosis and heart failure -- Mathematical simulations of sphingosine-1-phosphate actions on mammalian ventricular myofibroblasts and myocytes -- Extracellular matrix and cardiac disease: surgical and scientific perspectives -- The role or neurohumoral activation in cardiac fibrosis and heart failure -- Natriuretic peptides: critical regulators of cardiac fibroblasts and the extracellular matrix in the heart -- Cardiac tissue engineering for the treatment of heart failure post-infarction -- Mechanisms in cardiac valve failure and the development of tissue engineered heart valves -- Index.
  • 2010 CRCnetBASE
    Landrum, John Thomas.
    "Carotenoids are of great interest due to their essential biological functions in both plants and animals. However, the properties and functions of carotenoids in natural systems are surprisingly complex. With an emphasis on the chemical aspects of these compounds, Carotenoids: Physical, Chemical, and Biological Functions and Properties presents a broad overview and recent developments with respect to understanding carotenoid structure, electronic and photochemical properties, and the use of novel analytical methods in the detection and characterization of carotenoids and their actions. The text also explores LC/MS and LC/MS/MS techniques as well as new applications of PCR and molecular biology methodologies"--Book jacket.
  • 2005 CRCnetBASE
    Packer, Lester.
  • 2013 CRCnetBASE
    Kraemer, Klaus; Siems, Werner; Sommerburg, Olaf.
    Hepatic retinoid metabolism : what is known and what is not / Jason J. Yuen, Jian Zhang Yang and William S. Blaner -- Metabolism of vitamin A in white adipose tissue and obesity / Shanmugam M. Jeyakumar ... [et al.] -- Retinoids in the treatment of psoriasis / Neil Borkar, Alan Menter -- Age-related macular degeneration and carotenoids / Nilesh Kalariya, Werner G. Siems, Frederik van Kuijk -- Lutein, zeaxanthin and vision across the lifespan / Billy R. Hammond, Jr. -- Assuring vitamin A adequacy to prevent eye and ear disorders / Tripper Sauer, Susan Emmett, Keith P. West, Jr. -- Disturbed accumulation and abnormal distribution of macular pigment in retinal disorders / Tos TJM Berendschot, Peter Charbel Issa, Thomas Theelen -- Retinol and otitis media / Akeem Olawale Lasisi -- Carotenoids and mutagenesis / P. M. Eckl ... [et al.] -- Carotenoids and vitamin A in lung cancer prevention / Anita Ratnasari Iskandar, Xiang-Dong Wang -- Is the effect of [Beta]-carotene on prostate cancer cells dependent on their androgen sensitivity? / Joanna Dulinska-Litewka ... [et al.] -- Carotenoid intake and supplementation in cancer : pro and con / W.G. Siems ... [et al.] -- Vitamin A in lung development and function / Hans-Konrad Biesalski -- Vitamin A and carotenoids in lung diseases / Olaf Sommerburg, Werner Siems -- Chemopreventive effects of astaxanthin on inflammatory bowel disease and inflammation-related colon carcinogenesis / Masashi Hosokawa, Yumiko Yasui -- Carotenoids in laboratory medicine / Ingolf Schimke, Michael Vogeser -- Supply of vitamin A in developing countries / Klaus Kraemer -- Vitamin A intakes are below recommendations in a significant proportion of the population in the western world / Hans-Konrad Biesalski, Barbara Trösch, Petra Weber -- Critical appraisal of vitamin A supplementation program in India / Dechenla Tshering Bhutia, Saskia De Pee -- Consequences of common genetic variations on SS-carotene cleavage for vitamin A supply / Georg Lietz, Anthony Oxley, Christine Boesch-Saadatmandi.
  • 2014 Springer Protocols
    Bozhkov, Peter V.; Salvesen, G.
    General in vitro Caspase Assay Procedures -- Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity.- Global Identification of Caspase Substrates using PROTOMAP (PROtein TOpography and Migration Analysis Platform) -- Caspase-2 Protocols -- Caspase-14 Protocols -- Caspase Protocols in Caenorhabditis elegans -- Detecting Caspase Activity in Drosophila Larval Imaginal Discs -- Methods for the Study of Caspase Activation in the Xenopus laevis Oocyte and Egg Extract -- Caspase Protocols in Mice -- Measurement of Caspase Activation in Mammalian Cell Cultures -- Detection and Measurement of Paracaspase MALT1 Activity -- Leishmania Metacaspase: an Arginine-Specific Peptidase -- Purification, Characterization, and Crystallisation of Trypanosoma Metacaspases -- Monitoring the Proteostasis Function of the Saccharomyces cerevisiae Metacaspase Yca1 -- Plant Metacaspase Activation and Activity -- Preparation of Arabidopsis thaliana Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC.
  • 2013 ScienceDirect
    Eiden, Lee E.
    Through the use of extended graphical abstracts and some traditional text-only abstracts this collection provides, a record of and roadmap to, the research presented at The Tenth International Catecholamine Symposium (XICS) held in September of 2012. Organized around ten general themes, each is introduced by a short overview identifying interesting research programs, results and potential areas of growth. The collection is a roadmap to key research and future opportunities for new catecholamine research programs and will be of interest to neuroscientists and clinical neurologists interested in understanding the current and future state of catecholamine research..
  • 2012 Springer
    Frank, Philippe G.; Jasmin, Jean-François; Lisanti, Michael P.
    Lipid rafts, caveolae, and GPI linked proteins / Valerie L. Reeves, Candice M. Thomas, and Eric J. Smart -- Caveolae and the regulation of endocytosis / Anna L. Kiss -- Caveolin : role in cell signaling / Cecile Boscher and Ivan Robert Nabi -- Regulation of ENOS in caveolae / Chieko Mineo and Philip W. Shaul -- Recent developments in the interactions between caveolin and pathogens / Fabiana S. Machado ... [et al.] -- Caveolin and breast cancer : a new clinical perspective / Isabelle Mercier and Michael P. Lisanti -- Caveolin and prostate cancer progression / Michael R. Freeman, Wei Yang, and Dolores Di Vizio -- Caveolins and caveolae, roles in insulin signalling, and diabetes / Peter Strolfors -- Atherosclerosis, caveolae, and caveolin / Stephanos Pavlides ... [et al.] -- Caveolins and heart diseases / Mathivadhani Panneerselvam, Hemal H. Patel, and David M. Roth -- Caveolins and lung function / Nikolaos A. Maniatis ... [et al.].
    Also available: Print – 2012
  • 2010 Springer
    Perbal, Annick; Perbal, Bernard V.; Takigawa, Masaharu.
    1. A Recent Breakthrough in the CCN Field: Functional Interactions Between CCN2 and CCN3 are Uncovered / Bernard Perbal -- 2. Report on the Fifth International Workshop on the CCN Family of Genes / A. E. Irvine, B. Perbal, and H. Yeger -- 3. Asking the Right Questions: What Can the Structure of the CCN Protein Domains Tell Us? / Kenneth P. Holbourn, Bernard Perbal, and K. Ravi Acharya -- 4. Nucleophosmin/B23: A Multifunctional Regulator that Determines the Fate of CCN2 mRNA / Satoshi Kubota, Yoshiki Mukudai, Harumi Kawaki, Seiji Kondo, Takanori Eguchi, Kumi Sumiyoshi, Toshihiro Ohgawara, Tsuyoshi Shimo, and Masaharu Takigawa -- 5. The CCN Genes as the “Master” Regulators of Angiogenesis, Vasculogenesis, Fibrogenesis and Cell Differentiation/Fate Specification in Mechanical Force-Driven Developmental Processes and Pathological Events / Mary Hanna and Brahim Chaqour -- 6. A Monoclonal Antibody Approach to CCN5 Domain Analysis / Lan Wei, Frank McKeon, Joshua W. Russo, Joan Lemire, and John Castellot -- 7. Matricellular Protein CCN2 Produced by Tubular Epithelial Cells Plays a Pivotal Role in Renal Fibrogenesis / Hirokazu Okada, Tsutomu Inoue, and Hiromichi Suzuki -- 8. Cooperative Regulation of Cell Proliferation and Differentiation by CCN2 and CCN3 / Masaharu Takigawa, Harumi Kawaki, Satoshi Kubota, Karen M. Lyons, and Bernard Perbal -- 9. The Role of CCN3 in Mesenchymal Stem Cells / Ken-Ichi Katsube -- 10. Role of Connective Tissue Growth Factor in Cardiac Fibrosis / Daiji Kawanami, Saptarsi M. Haldar, and Mukesh K. Jain -- 11. Gene Expression of CCN Family Members in Young and Aged Human Skin In Vivo / Taihao Quan, Sharon Shin, Zhaoping Qin, and Gary J. Fisher -- 12. Global Expression Profiling Reveals a Role for CTGF/CCN2 in Lactogenic Differentiation of Mouse Mammary Epithelial Cells / Weihan Wang, Cynthia Jose, Nicholas Kenney, Bethanie Morrison, and Mary Lou Cutler -- 13. CCN3 (NOV): A Negative Regulator of CCN2 (CTGF) Activity and an Endogenous Inhibitor of Fibrosis in Experimental Diabetic Nephropathy / Bruce. L. Riser, Feridoon Najmabadi, Bernard Perbal, Jo Ann Rambow, Melisa L. Riser, Ernest Sukowski, Herman Yeger, Sarah C. Riser, and Darryl R. Peterson -- 14. Inhibitors of Connective Tissue Growth Factor (CCN2)-Mediated Fibrogenesis: Underlying Mechanisms and Prospects for Anti-fibrotic Therapy / David R. Brigstock -- 15. CCN3 Promotes Melanoma Progression by Regulating Integrin Expression, Adhesion and Apoptosis Induced by Cytotoxic Drugs / Viviana Vallacchi, Maria Daniotti, Annamaria De Filippo, Licia Rivoltini, Bernard Perbal, and Monica Rodolfo -- 16. CCN3: A NOVel Growth Factor in Leukaemia / Lynn McCallum and Alexandra E Irvine -- 17. Prognostic Relevance of CCN3 in Bone Sarcomas / Bernard Perbal, Noureddine Lazar, Diana Zambelli, Monia Zuntini, Massimo Serra, Jose Antonio Lopez-Guerrero, Antonio Llombart-Bosch, Piero Picci, and Katia Scotlandi -- 18. CCN6 Regulates Breast Cancer Growth and Invasion Through Modulation of IGF Signaling and Epithelial to Mesenchymal Transition / Anupama Pal, Wei Huang, and Celina G. Kleer -- 19. Novel Transcriptional Regulation of CCN2/CTGF by Nuclear Translocation of MMP3 / Takanori Eguchi, Satoshi Kubota, Kazumi Kawata, Yoshiki Mukudai, Junji Uehara, Toshihiro Ohgawara, Soichiro Ibaragi, Akira Sasaki, Takuo Kuboki, and Masaharu Takigawa -- Fifth International Workshop on the CCN Family of Genes: Abstracts and Posters October 18-22, 2008.
  • 2007 Springer
    Calderwood, Stuart K.
    Surveys the knowledge concerning the expression and function of stress proteins in different organisms, ranging from prokaryotes to humans. This book provides an overview of the diversity and complex evolutionary history of cell stress proteins and describes their function and expression in different eukaryote models.
  • 2005 Springer
    Limbird, Lee E.
    Also available: Print – 2005
  • 2014 Springer Protocols
    Alexandrov, Kirill; Johnston, Wayne A.
    Production of eukaryotic cell-free lysate from Leishmania tarentolae / Wayne A. Johnston and Kirill Alexandrov -- Bioinformatics analysis and optimization of cell-free protein synt / Alexander A. Tokmakov ... [et al.] -- Cell-free expression screen to identify fusion tags for improved protein expression / Andrew Kralicek -- One-pot, microscale cell-free enzyme expression and screening / Aarthi Chandrasekaran and Anup K. Singh -- Cell-free translation of biofuel enzymes / Taichi E. Takasuka ... [et al.] -- Cloning-independent expression and screening of enzymes using cell-free protein synthesis systems / Yong-Chan Kwon, Jae-Kwang Song, and Dong-Myung Kim -- High-level cell-free production of membrane proteins with nanodiscs / Christian Roos ... [et al.] -- Cell-free protein-based enzyme discovery and protein-ligand interaction study / Sabrina Guillemer ... [et al.] -- Human cell extract-derived cell-free systems for virus synthesis / Tominari Kobayashi, Kodai Machida, and Hiroaki Imataka -- Cell-free protein synthesis in microfluidic 96-well plates / Kirsten Jackson, Ruba Khnouf, and Z. Hugh Fan -- Preparation of multiple site-specific mutant proteins for NMR studies by PCR-Directed cell-free protein synthesis / Kiyoshi Ozawa and Ruhu Qi -- Site-specific incorporation of unnatural amino acids into proteins by cell-free protein synthesis / Kiyoshi Ozawa and Choy Theng Loh -- In vitro translation of papillomavirus authentic and codon-modified L1 capsid gene mRNAs in mouse keratinocyte cell-free lysate / Kong-Nan Zhao -- Optimized yeast cell-free lysate system for in vitro translation of human virus mRNA / Xiao Wang, Liang Zhao, and Kong-Nan Zhao -- In vitro translation-based protein kinase substrate identification / Szilvia K. Nagy and Tamás Mészáros -- Preparation of protein arrays using cell-free protein expression / Elizabeth A. Cook and Mingyue He -- Posttranscriptional control of protein synthesis in Drosophila S2 cell-free system / Motoaki Wakiyama and Shigeyuki Yokoyama -- Cell-free membrane protein expression / Tomomi Kimura-Soyema, Mikako Shirouzu, and Shigeyuki Yokoyama -- PURE System for Protein Production / Yoshihiro Shimizu ... [et al.] -- Cell-free protein synthesis system from insect cells / Toru Ezure, Takashi Suzuki, and Eiji Ando -- Cell-free expression platform for production of protein microarrays / Xristo Zárate and David W. Galbraith.
  • 2010 Springer
    Adams, Peter D.; Sedivy, John M.
  • 2012 ScienceDirect
    Gilbert, H. J.
    Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume in the Methods in Enzymology series comprehensively covers this topic.
  • 2014 ScienceDirect
    Galluzzi, Lorenzo; Kroemer, Guido.
  • 2009 Springer
    Antos, L. K.; Hofmann, Franz; Schmidt, Harald H. H. W.; Stasch, Johannes-Peter.
  • 2006 Springer
    Braakman, Ineke; Makarow, Marja.
  • 2013 Springer
    Macario, Alberto J. L.; Cappello, Francesco; Conway de Macario, Everly.
    This Brief provides a concise review of chaperonopathies, i.e., diseases in which molecular chaperones play an etiologic-pathogenic role. Introductory chapters deal with the chaperoning system and chaperoning teams and networks, HSP-chaperone subpopulations, the locations and functions of chaperones, and chaperone genes in humans. Other chapters present the chaperonopathies in general, including their molecular features and mechanistic classification into by defect, excess, or mistake. Subsequent chapters discuss the chaperonopathies in more detail, focusing on their distinctive characteristic.
  • 2010
    Van, Christopher; Chen, James K.; Cimprich, Karlene; Straight, Aaron; Wang, Teresa.
    Stalled replication forks activate and are stabilized by the ATR-mediated checkpoint, but ultimately they must also recover from the arrest. While primed ssDNA is sufficient for checkpoint activation, it is still unknown how this signal is generated at a stalled replication fork. Furthermore, it is not clear how recovery and fork restart occur in higher eukaryotes. Using Xenopus egg extracts, we show that DNA replication continues at a stalled fork through the synthesis and elongation of new primers independent of the checkpoint. This synthesis is dependent on the activity of PCNA, Pol[delta] and Pol[epsilon], and it contributes to the phosphorylation of Chk1. We also used defined DNA structures to show that for a fixed amount of ssDNA, increasing the number of primer-template junctions strongly enhances Chk1 phosphorylation. These results suggest that new primers are synthesized at stalled replication forks by the leading and lagging strand polymerases and that accumulation of these primers may contribute to checkpoint activation.
  • 2013 Springer
    Shibasaki, Masakatsu.
    At the forefront of life sciences today is the emerging discipline of chembiomolecular science. This new term describes the integration of the frontier fields of chemical biology, chemistry, and pharmacology. Chembiomolecular science aims to elucidate new biological mechanisms as potential drug targets and enhance the creation of new drug therapies. This book comprises the proceedings of the Uehara Memorial Foundation Symposium 2011, which focused on the most recent advances in chembiomolecular science made by leading experts in the field. The book is divided into three main topics. The first is the chemical approach to understanding complex biological systems on a molecular level using chemical compounds as a probe. The second describes the biological approach used to develop new lead drug compounds. The third focuses on the biological system that serves as the potential drug target, the beginning step in the process of developing new drugs. Replete with the latest research, the book will draw the attention of all scientists interested in the synergies between chemistry and biology to elucidate life on a molecular level and to promote drug discovery. Ultimately, the book helps promote the understanding of biological functions at the molecular level and create new pharmaceuticals that can contribute to improving human health.
  • 2015 Springer Protocols
    Hempel, Jonathan E.; Hong, Charles C.; Williams, Charles H.
    Identification of Therapeutic Small Molecule Leads in Cultured Cells Using Multiplexed Pathway Reporter Read-outs -- Applying the Logic of Genetic Interaction to Discover Small Molecules That Functionally Interact with Human Disease Alleles -- Construction and Application of a Photo-cross-linked Chemical Array -- High Content Screening for Modulators of Cardiac Differentiation in Human Pluripotent Stem Cells -- Small Molecule High-Throughput Screening Utilizing Xenopus Egg Extract -- Fission Yeast-based High-throughput Screens for PKA Pathway Inhibitors and Activators -- A Method for High-throughput Analysis of Chronological Aging in Schizosaccharomyces pombe -- Protocols for the Routine Screening of Drug Sensitivity in the Human Parasite Trichomonas vaginalis -- Chemical Genetic Screens Using Arabidopsis thaliana Seedlings Grown on Solid Medium -- Small Molecule Screening Using Drosophila Models of Human Neurological Disorders -- High-throughput Small Molecule Screening in Caenorhabditis elegans -- Whole-organism Screening for Modulators of Fasting Metabolism Using Transgenic Zebrafish -- High Content Screening for Modulators of Cardiovascular or Global Developmental Pathways in Zebrafish -- Extraction Methods of Natural Products from Traditional Chinese Medicines -- Bioassay-guided Identification of Bioactive Molecules from Traditional Chinese Medicines -- NMR Screening in Fragment-Based Drug Design: A Practical Guide -- Practical Strategies for Small Molecule Probe Development in Chemical Biology -- Principal Component Analysis as a Tool for Library Design: A Case Study Investigating Natural Products, Brand-name Drugs, Natural Product-like Libraries, and Drug-like Libraries -- Small Molecule Library Screening by Docking with PyRx -- Fluorous Photoaffinity Labeling to Probe Protein-Small Molecule Interactions -- Identification of the Targets of Biologically Active Small Molecules Using Quantitative Proteomics -- Drug Affinity Responsive Target Stability (DARTS) for Small Molecule Target Identification -- Chemical Genomic Profiling via Barcode Sequencing to Predict Compound Mode of Action -- Image-Based Prediction of Drug Target in Yeast.
  • 2006 Springer
    Jaroch, S.; Weinmann, Hilmar.
  • 2012 Springer Protocols
    Zanders, Edward D.
    Overview of chemical genomics and proteomics / Edward D. Zanders -- Exploring chemical space : recent advances in chemistry / Yung-Sing Wong -- In silico design of small molecules / Paul H. Bernardo and Joo Chuan Tong -- Surface plasmon resonance for proteomics / Nico J. de Mol -- Biomimetic affinity ligands for immunoglobulins based on the multicomponent Ugi reaction / Graziella El Khoury, Laura A. Rowe, and Christopher R. Lowe -- Preparation of photo-cross-linked small molecule affinity matrices for affinity selection of protein targets for biologically active small molecules -- Profiling cellular myristoylation and palmitoylation using [omega]-alkynyl fatty acids -- Fluorescence labels in kinases: A high-throughput kinase binding assay for the identification of DFG-out binding ligands / Hiroshi Takayama, Takashi Moriya, and Naoki Kanoh -- Electrochemical aptamer sensor for small molecule assays / Xin Liu [and others] -- Miniaturized, microarray-based assays for chemical proteomic studies of protein function / Jonathan M. Blackburn, Aubrey Shoko, and Natasha Beeton-Kempen -- Glycan microarrays / Xuezheng Song [and others] -- Resolving bottlenecks for recombinant protein expression in E. coli / Yoav Peleg and Tamar Unger -- Recombinant protein expression in the baculovirus-infected insect cell system / Tamar Unger and Yoav Peleg -- Systems for the cell-free synthesis of proteins / Lei Kai [and others] -- Protein identification by MALDI-TOF mass spectrometry / Judith Webster and David Oxley.
  • 2009
    Brennan, Ian Michael; Herschlag, Daniel; Straight, Aaron; Theriot, Julie A.
    During cell division, chromosome segregation must be coordinated with cell cleavage so that cytokinesis occurs after chromosomes have been safely distributed to each spindle pole. Polo like kinase 1 (Plk1) is an essential kinase that regulates spindle assembly, mitotic entry and chromosome segregation but because of its many mitotic roles it has been difficult to specifically study its post-anaphase functions. Small molecule inhibitors were used to block Plk1activity at anaphase onset and demonstrate that Plk1controls both spindle elongation and cytokinesis. Plk1 inhibited cells failed to assemble a contractile ring and contract the cleavage furrow due to a defect in Rho and Rho-GEF localization to the division site. Plk1 inhibition did not affect anaphase A chromosome to pole movement but blocked anaphase B spindle elongation. Anaphase B is thought to result from the coordinated activities of microtubule-sliding motors that drive the poles further apart and changes in spindle microtubule dynamics. Plk1 is unlikely to control anaphase B through regulation of a spindle kinesin because inhibition of known motor proteins failed to recapitulate the Plk1 depletion phenotype. Instead, Plk1 inhibition caused a significant decrease in microtubule growth rate during metaphase and early anaphase, indicating a role for Plk1 in regulating microtubule dynamics. Consistent with an inhibition of microtubule growth rate, Plk1 inhibition reduced the rate of poleward microtubule flux in metaphase spindles and caused a reduction in metaphase spindle length that could be reversed by microtubule stabilization with taxol. These data suggest a model in which Plk1 accelerates microtubule growth during mitosis to maintain spindle length and drive anaphase B spindle elongation.
  • 2013 Springer Protocols
    Banghart, Matthew R.
    Engineering k(+) channels using semisynthesis -- Chemical derivatization and purification of peptide-toxins for probing ion channel complexes -- Using yeast to study potassium channel function and interactions with small molecules -- A FLIPR assay for evaluating agonists and antagonists of GPCR heterodimers -- Characterizing caged molecules through flash photolysis and transient absorption spectroscopy -- Characterization of one- and two-photon photochemical uncaging efficiency -- Photochromic potassium channel blockers: Design and electrophysiological characterization -- A (1)h NMR assay for measuring the photostationary states of photoswitchable ligands -- Developing a photoreactive antagonist -- Development and in vitro characterization of ratiometric and intensity-based fluorescent ion sensors -- Characterization of voltage-sensitive dyes in living cells using two-photon excitation -- Characterization and validation of fluorescent receptor ligands: A case study of the ionotropic serotonin receptor -- Imaging single synaptic vesicles in mammalian central synapses with quantum dots -- Directed evolution of protein-based neurotransmitter sensors for MRI.
  • 2012 Springer Protocols
    Bantscheff, Marcus; Drewes, Gerard.
    Mass spectrometry-based chemoproteomic approaches -- Chemical proteomics in drug discovery -- Compound immobilization and drug-affinity chromatography -- Affinity-based chemoproteomics with small molecule-peptide conjugates -- A chemical proteomic probe for detecting dehydrogenases: Catechol rhodanine -- Probing proteomes with benzophenone photoprobes -- Biotinylated probes for the analysis of protein modification by electrophiles -- Profiling of methyltransferases and other S-adenosyl-l: -homocysteine-binding proteins by capture compound mass spectrometry -- Identifying cellular targets of small-molecule probes and drugs with biochemical enrichment and SILAC -- Determination of kinase inhibitor potencies in cell extracts by competition binding assays and isobaric mass tags -- Affinity-based profiling of dehydrogenase subproteomes -- Probing the specificity of protein-protein interactions by quantitative chemical proteomics -- Fluorescence-based proteasome activity profiling -- Chemical cross-linking and high-resolution mass spectrometry to study protein-drug interactions -- Monitoring ligand modulation of protein-protein interactions by chemical cross-linking and high-mass MALDI mass spectrometry -- Time-controlled transcardiac perfusion crosslinking for in vivo interactome studies -- Ligand discovery using small-molecule microarrays -- Working with small molecules : preparing and storing stock solutions and determination of kinetic solubility -- A database for chemical proteomics : ChEBI -- Working with small molecules: Rules-of-thumb of Drug likeness.
  • 2014 CRCnetBASE
    Lundblad, Roger L.
  • 2010
    Sellmyer, Mark Anthony; Chu, Gilbert; Contag, Christopher H.; Ferrell, James Ellsworth; Mitchell, Beverly S.; Wandless, Thomas.
    A detailed understanding of living systems requires tools to examine and manipulate biological processes. Small molecules and optical imaging technologies are uniquely suited for this purpose. Small molecules enable the specific manipulation of biomolecular targets, and optical imaging permits the real-time observation of molecular and cellular processes in vivo. This dissertation describes a combination of chemical tool development and imaging strategies to address the following biological problems: 1) specific modification of the genome 2) exquisite control of protein function 3) observing cell-cell interactions in living animals. Chapter One describes a technology for targeted gene modification via the induction of double strand breaks in genomic DNA. The chapter begins with an overview of the field of gene targeting, and documents the design, synthesis, and testing of a novel method for high efficiency homologous recombination. The method relies on engineering two reagents, a small molecule DNA targeting element and a nuclease fusion protein. The targeting element, a peptide nucleic acid (PNA), was designed and synthesized to target DNA via Watson-Crick base pairing. The PNA also was covalently linked to the small molecule, trimethoprim, to recruit a DHFR nuclease fusion protein to a specific DNA site. Our studies show that the individual interactions of PNA/DNA and of PNA/nuclease can readily occur. Further, the ternary complex of PNA, DNA, and nuclease can form in solution. Chapters Two, Three, and Four describe the development and further characterization of a general method to perturb protein stability and function. Briefly, an unstable protein domain, termed a destabilizing domain (DD), can confer instability to a fused protein of interest and promote its rapid degradation. This instability can be rescued by the addition of a small molecule, Shield-1. Our work in Chapter Two describes the use of this system to regulate protein function in living mammals. In one example, we show that regulation of a secreted protein, the immunomodulatory cytokine IL-2, can control tumor burden in mouse models. Additionally, we used the DD to control the function of TNF-[Alpha] after systemic delivery to a tumor. Chapter Three expands on these in vivo efforts by employing the system to regulate secreted FGF2, an important modulator of bone formation, for skeletal tissue engineering. Shield-1 induction of FGF2 causes induction of bone formation in a calvarial-defect model. Chapter Four presents our observations on the behavior of the DD in various cellular environments --the cytoplasm, nucleus, endoplasmic reticulum, and mitochondria- and in the presence of small molecules that modulate protein production, degradation, and local protein quality control machinery. These data indicate that the levels of the DD, in the presence and absence ligand, is dependent on its subcellular locale and protein homeostasis machinery. The fifth chapter of this dissertation reports the development of a method to assess spatiotemporal cell-cell relationships in real-time and in living animals. The method is based on small-molecule diffusion of an activatable substrate between two populations of cells, thus allowing assessment of cell-cell proximity in vivo via bioluminescence imaging. One cell population catalyzes the release of a caged luciferin. The free luciferin can diffuse to a nearby population of cells expressing luciferase capable of light-emitting catalysis. Thus the luciferase cells in closest proximity to the "pool" of free luciferin emit the most light. We demonstrate the utility of this system in vitro and in vivo and are currently investigating its use for the detection of cancer and early metastatic disease in mouse models.
  • v. 1, 1967.
    Florkin, Marcel; Kidder, George Wallace; Scheer, Bradley T.
    Status: Not Checked OutLane Catalog Record
    v. 1. Protozoa -- v. 2. Porifera, Coelenterata, and Platyhelminthes -- v. 3. Echinodermata, Nematoda, and Acanthocephala -- v. 4. Annelida, Echiura, and Sipuncula -- v. 5-6. Arthropoda -- v. 7. Mollusca -- v. 8. Deuterostomians, cyclostomes, and fishes -- v. 9. Amphibia and Reptilia -- v. 10. Aves.
  • 2005 ScienceDirect
    Garg, Hari G.; Hales, Charles A.; Linhardt, Robert J.
  • v. 1-3, 1970.
    Balazs, Endre A.
    Status: Not Checked OutLane Catalog Record
    v. 1. Collagen, basal laminae elastin --v. 2. Glycosaminoglycans and proteoglycans --v. 3. Structural organization and function of the matrix.
  • 2013 CRCnetBASE
    Brahmachari, Goutam.
    "Natural products play crucial roles in modern drug development and constitute a prolific source of novel lead compounds or pharmacophores for ongoing drug discovery programs. Chemistry and Pharmacology of Naturally Occurring Bioactive Compounds presents cutting-edge research in the chemistry of bioactive natural products and demonstrates how natural product research continues to make significant contributions in the discovery and development of new medicinal entities."--Page [4] of cover.
  • 2015 Springer
    Moore, P. K.; Whiteman, Matt.
    This book puts hydrogen sulfide in context with other gaseous mediators such as nitric oxide and carbon monoxide, reviews the available mechanisms for its biosynthesis and describes its physiological and pathophysiological roles in a wide variety of disease states. Hydrogen sulfide has recently been discovered to be a naturally occurring gaseous mediator in the body. Over a relatively short period of time this evanescent gas has been revealed to play key roles in a range of physiological processes including control of blood vessel caliber and hence blood pressure and in the regulation of nerve function both in the brain and the periphery. Disorders concerning the biosynthesis or activity of hydrogen sulfide may also predispose the body to disease states such as inflammation, cardiovascular and neurological disorders. Interest in this novel gas has been high in recent years and many research groups worldwide have described its individual biological effects. Moreover, medicinal chemists are beginning to synthesize novel organic molecules that release this gas at defined rates with a view to exploiting these new compounds for therapeutic benefit.
  • 2007 Springer
    Harrison, Jeffrey K.; Lukacs, Nicholas W.
  • 2016 ScienceDirect
    Handel, Tracy M.
  • 2013 Springer Protocols
    Cardona, Astrid E.; Ubogu, Eroboghene E.
    Chemokines constitute a large family of structurally similar cytokines that contain a signature of conserved cysteine residues joined by disulfide bridges. Binding of chemokines to specific G protein-coupled receptors followed by downstream signaling defines their biological function. Initially, chemoattraction was the key function linked to chemokines/chemokine receptors; however, in recent years, it has become clear that chemokine ligand-receptor interactions can also modulate cellular activation, survival, and proliferation, among other functions in homeostatic and diseased states. Importantly, major advances in our understanding of chemokine biology have led to chemokine receptors becoming specific therapeutic targets with great potential. In Chemokines: Methods and Protocols, expert researchers provide practical information regarding experimental models and state of the art protocols used to delineate chemokine/chemokine receptor function and their applications in health and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Chemokines: Methods and Protocols aims to reveal key protocols of functional and descriptive chemokine ligand/receptor assays that will be of practical significance to graduate students, post-doctoral fellows, trainees, and researchers in academia and industry.
  • 2013 Springer Protocols
    Scriba, Gerhard K. E.
    Chiral recognition in separation science : an overview / Gerhard K.E. Scriba -- Enantioseparations by thin-layer chromatography / Massimo Del Bubba [and others] -- Gas-chromatographic enantioseparation of unfunctionalized chiral hydrocarbons : an overview / Volker Schurig and Diana Krieidler -- HPLC enantioseparation on cyclodextrin-based chiral stationary phases / Yong Wang and Siu Choon Ng -- Enantioseparations by high-performance liquid chromatography using polysaccharide-based chiral stationary phases : an overview / Bezhan Chankvetadze -- Common screening approaches for efficient analytical method development in LC and SFC on columns packed with immobilized polysaccharide-derived chiral stationary phases / Pilar Franco and Tong Zhang -- Chiral separations by HPLC on immobilized polysaccharide chiral stationary phases / Imran Ali, Zeid A. AL-Othman, and Hassan Y. Aboul-Enein -- Enantioseparations by high-performance liquid chromatography using macrocyclic glycopeptide-based chiral stationary phases : an overview / István Ilisz [and others] -- Enantioseparations of primary amino compounds by high-performance liquid chromatography using chiral crown ether-based chiral stationary phase / Myung Ho Hyun -- Screening of Pirkle-type chiral stationary phases for HPLC enantioseparations / Gregory K. Webster and Ted J. Szczerba -- Enantioseparations by high-performance liquid chromatography based on chiral ligand-exchange / Benedetto Natalini, Roccaldo Sardella, and Federica Ianni -- Enantioseparations by high-performance liquid chromatography using molecularly imprinted polymers / David A. Spivak -- Chiral mobile phase additives in HPLC enantioseparations / Lushan Yu, Shengjia Wang, and Su Zeng -- Chiral benzofurazan-derived derivatization reagents for indirect enantioseparations by HPLC / Toshimasa Toyo'oka -- Separation of racemic 1-(9-Anthryl)-2,2,2-trifluoroethanol by sub-/supercritical fluid chromatography / Xiqin Yang, Leo Hsu, and Gerald Terfloth -- Chiral separations by simulated moving bed method using polysaccharide-based chiral stationary phases / Toshiharu Minoda -- Enantioseparations by capillary electrophoresis using cyclodextrins as chiral selectors / Gerhard K.E. Scriba and Pavel Jáč -- Application of dual cyclodextrin systems in capillary electrophoresis enantioseparations / Anne-Catherine Servais and Marianne Fillet -- Enantioseparations in nonaqueous capillary electrophoresis using charged cyclodextrins / Anne-Catherine Servais and Marianne Fillet -- Use of macrocyclic antibiotics as the chiral selectors in capillary electrophoresis / Chengke Li and Jingwu Kang -- Application of polymeric surfactants in chiral micellar electrokinetic chromatography (CMEKC) and CMEKC coupled to mass spectrometry / Jun He and Shahab A. Shamsi -- Cyclodextrin-modified micellar electrokinetic chromatography for enantioseparations / Wan Aini Wan Ibrahim, Dadan Hermawan, and Mohd Marsin Sanagi -- Cyclodextrin-mediated enantioseparation in microemulsion electrokinetic chromatography / Claudia Borst and Ulrike Holzgrabe -- Chiral separations by capillary electrophoresis using proteins as chiral selectors / Jun Haginaka -- Enantioseparation by chiral ligand-exchange capillary electrophoresis / Yi Chen and Lijuan Song -- Experimental design methodologies in the optimization of chiral CE or CEC separations : an overview / Bieke Dejaegher, Debby Mangelings, and Yvan Vander Heyden -- Chiral capillary electrophoresis-mass spectrometry / Elena Domínguez-Vega, Antonio L. Crego, and Maria Luisa Marina -- Application of chiral ligand-exchange stationary phases in capillary electrochromatography / Martin G. Schmid -- Polysaccharide-derived chiral stationary phases in capillary electrochromatography enantioseparations / Zhenbin Zhang, Hanfa Zou, and Junjie Ou -- Open tubular molecular imprinted phases in chiral capillary electrochromatography / Won Jo Cheong and Song Hee Yang -- Enantioseparations in capillary electrochromatography using sulfated poly [beta]-cyclodextrin-modified silica-based monolith as stationary phase / Ruijuan Yuan and Guosheng Ding -- Cyclodextrin-mediated enantioseparations by capillary electrochromatography / Dorothee Wistuba and Volker Schurig.
  • 2006 Springer
    Grimm, Bernhard.
  • 2002 CRCnetBASE
    Guardiola, Francesc.
  • 2010 Springer
    Harris, James R.
    Cholesterol-protein interaction: methods and cholesterol reporter molecules / G. Gimpl -- Cholesterol in Alzheimer's disease and other amyloidogenic disorders / J.R. Harris and N.G. Milton -- Cholesterol-binding viral proteins in virus entry and morphogenesis / C. Schroeder -- Sterol-protein interactions in cholesterol and bile acid synthesis / E. De Fabiani ... [et al.] -- Cholesterol oxidase: structure and function / A. Vrielink -- Oxysterol-binding proteins / N.D. Ridgway -- High density lipoprotein structure-function and role in reverse cholesterol transport / S. Lund-Katz and M.C. Phillips -- Lipoprotein modification and macrophage uptake: role of pathologic cholesterol transport in atherogenesis / Y.I. Miller ... [et al.] -- Cholesterol interaction with proteins that partition into membrane domains: an overview / R.M. Epand ... [et al.] -- Caveolin, sterol carrier protein-2, membrane cholesterol-rich microdomains and intracellular cholesterol trafficking / F. Schroeder ... [et al.] -- Cholesterol in Niemann-Pick type C disease / X. Bi and G. Liao -- Protein mediators of sterol transport across intestinal brush border membrane / J.M. Brown and L. Yu -- Cholesterol at the endoplasmic reticulum: Roles of the sigma-1 receptor chaperone and implications thereof in human diseases / T. Hayashi and T.P. Su -- Prominin-1: a distinct cholesterol-binding membrane protein and the organisation of the apical plasma membrane of epithelial cells / D. Corbeil ... [et al.] -- Mammalian StAR-related lipid transfer (START) domains with specificity for cholesterol: Structural conservation and mechanism of reversible binding / P. Lavigne, R. Najmanivich and J.G. Lehoux -- Membrane cholesterol in the function and organization of G-protein coupled receptors / Y.D. Paila and A. Chattopadhyay -- Cholesterol effects on nicotinic acetylcholine receptor: cellular aspects / F.J. Barrantes -- Cholesterol and myelin biogenesis / G. Saher and M. Simons -- Cholesterol and ion channels / I. Levitan ... [et al.] -- The cholesterol-dependent cytolysin family of gram-positive bacterial toxins / A.P. Heuck, P.C. Moe and B.B. Johnson -- Cholesterol specificity of some heptameric beta-barrel pore-forming bacterial toxins: Structural and functional aspects / J.R. Harris and M. Palmer -- Cholesterol-binding toxins and anti-cholesterol antibodies as structural probes for cholesterol localization / Y. Ohno-Iwashita ... [et al.].
  • 2012 Wiley
    Barrantes, Francisco J.; Levitan, Irena.
    Cholesterol trafficking and distribution between cellular membranes / Daniel Wustner, Lukasz M. Solanko, Frederik W. Lund -- Cholesterol regulation of membrane protein function by changes in bilayer physical properties : an energetic perspective / Jens A. Lundbaek and Olaf S. Andersen -- Insights into structural determinants of cholesterol sensitivity of Kir channels / Avia Rosenhouse-Dantsker and Irena Levitan -- Role for lipid rafts in the regulation of store-operated Ca2+ channels / Hwei L. Ong and Indu S. Ambudkar -- Cholesterol regulation of cardiac ion channels / Elise Balse, Stephane Hatem and Stanley Nattel -- Differential contribution of BK subunits to nongenomic regulation of channel function by steroids / Alex M. Dopico, Anna N. Bukiya, and Aditya K. Singh -- Regulation of K+ channels by cholesterol-rich membrane domains in immune system / Núria Comes and Antonio Felipe -- Indirect channel regulation by cholesterol : the role of caveolae and caveolins in regulating KATP channel function / Caroline Dart -- Regulation of the nicotinic acetylcholine receptor by cholesterol as a boundary lipid / Francisco J. Barrantes -- Specific and non-specific regulation of GPCR function by cholesterol / Gerald Gimpl and Katja Gehrig-Burger -- Structural insights into cholesterol interactions with G protein-coupled receptors / Jeremiah S. Joseph, Enrique E. Abola, and Vadim Cherezov -- Membrane cholesterol : implications in receptor function / Sandeep Shrivastava and Amitabha Chattopadhyay -- The role of cholesterol and lipid rafts in regulation of TLR receptors / Ruxana T. Sadikot.
  • pt. A-C, 2004. ScienceDirect
    pt. B ScienceDirect
    pt. C ScienceDirect
    Allis, C. David; Wu, Carl.
    Also available: Print – pt. A-C, 2004.
  • 2007 Springer
    Dasgupta, Dipak; Kundu, Tapas K.
  • 2010 Springer
    Albrecht, Urs.
  • pt. A-B, 2015 ScienceDirect
    pt. B ScienceDirect
    Sehgal, Amita.
    Two new volumes of Methods in Enzymology continue the legacy of this premier serial with quality chapters authored by leaders in the field. Circadian Rhythms and Biological Clocks Part A and Part B is an exceptional resource for anybody interested in the general area of circadian rhythms. As key elements of timekeeping are conserved in organisms across the phylogenetic tree, and our understanding of circadian biology has benefited tremendously from work done in many species, the volume provides a wide range of assays for different biological systems. Protocols are provided to assess clock function, entrainment of the clock to stimuli such as light and food, and output rhythms of behavior and physiology. This volume also delves into the impact of circadian disruption on human health. Contributions are from leaders in the field who have made major discoveries using the methods presented here.
  • 2015 Springer
    Igaz, Peter.
    MicroRNAs as the endogenous mediators of RNA interference have experienced an unprecedented career in recent years, highlighting their pathogenic, diagnostic and potential therapeutic relevance. Beside tissue microRNAs, they are also found in body fluids, most notably in blood. Significant differences of circulating microRNA levels have been found in various diseases, making them candidates for minimally invasive markers of disease, for example tumor malignancy. The book focuses on the potential diagnostic applicability of circulating microRNAs in various diseases and their potential biological significance.
  • 2015 Springer
    Gahan, Peter B.
  • 2011 Springer Protocols
    Turksen, Kursad.
    Measuring size-dependent permeability of the tight junction using PEG profiling / Christina M. Van Itallie and James M. Anderson -- Biochemical analysis of claudin-binding compatibility / Christina Ward and Michael Koval -- Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines / Alan S.L. Yu -- Tight junction, intercellular seal as a cell signaling player : protocols for examination of its status / Makoto Osanai -- Interactions between Clostridium perfringens enterotoxin and claudins / Susan L. Robertson and Bruce A. McClane -- Biophysical methods to probe claudin-mediated adhesion at the cellular and molecular level / Sri Ram Krishna Vedula [and others] -- Detection of tight junction barrier function in vivo by biotin / Lei Ding [and others] -- Coculture method to examine interactions between claudin isoforms in tight junction-free HEK293 cells and tight junction-bearing MDCK II cells / Tetsuichiro Inai -- Claudin-4 : functional studies beyond the tight junction / Holly A. Eckelhoefer [and others] -- Methods to analyze subcellular localization and intracellular trafficking of claudin-1 / P. Jaya Kausalya and Walter Hunziker -- Claudin family proteins in Caenorhabditis elegans / Jeffrey S. Simske and Jeff Hardin -- In vivo imaging of tight junctions using claudin-EGFP transgenic medaka / Tatsuo Miyamoto, Mikio Furuse, and Makoto Furutani-Seiki -- Claudins in a primary cultured puffer fish (Tetraodon nigroviridis) gill epithelium / Phuong Bui and Scott P. Kelly -- Manipulating claudin expression in avian embryos / Michelle M. Collins and Aimee K. Ryan -- Identification of claudins by western blot and immunofluorescence in different cell lines and tissues / Lorenza Gonzalez-Mariscal, Erika Garay, and Miguel Quiros -- Expression and function of claudins in hepatocytes / Takashi Kojima and Norimasa Sawada -- Analysis of changes in the expression pattern of claudins using salivary acinar cells in primary culture / Junko Fujita-Yoshigaki -- Development of biological tools to study claudins in the male reproductive tract / Daniel G. Cyr [and others] -- Using molecular tracers to assess the integrity of the intestinal epithelial barrier in vivo / Julian A. Guttman -- Laboratory methods in the study of endometrial claudin-4 / Paulo Serafini [and others] -- Role of claudins in oxidant-induced alveolar epithelial barrier dysfunction / Yu Sun, Richard D. Minshall, and Guochang Hu -- Tracing the endocytosis of claudin-5 in brain endothelial cells / Svetlana M. Stamatovic, Richard F. Keep, and Anuska V. Andjelkovic -- Quantitative in situ analysis of claudin expression at the blood-retinal barrier / Heping Xu and Janet Liversidge -- MMP-mediated disruption of claudin-5 in the blood-brain barrier of rat brain after cerebral ischemia / Yi Yang and Gary A. Rosenberg -- Claudin-5 expression in in vitro models of the blood-brain barrier / Itzik Cooper, Katayun Cohen-Kashi-Malina, and Vivian I. Teichberg -- HIV-1-induced alterations of claudin-5 expression at the blood-brain barrier level / Ibolya E. Andras and Michal Toborek -- Enhanced immunohistochemical resolution of claudin proteins in glycolmethacrylate-embedded tissue biopsies / Jane E. Collins [and others] -- Claudin-16/paracellin-1, cloning, expression, and its role in tight junction functions in cancer and endothelial Cells / Tracey A. Martin and Wen G. Jiang -- Dynamics of claudins expression in colitis and colitis-associated cancer in rat / Yoshiaki Arimura, Kanna Nagaishi, and Masayo Hosokawa -- Anti-claudin-4-conjugated highly luminescent nanoparticles as biological labels for pancreatic cancer sensing / Ken-Tye Yong.
  • 2012 Springer Protocols
    Kalish, Heather; Phillips, Terry M.
    Overview of CE in clinical analysis / David S. Hage -- Monitoring of arrays of amino acids in clinical samples using capillary electrophoresis with contactless conductivity detection / Petr Tu°ma and Karel Štulík -- Selective determination of sulfates, sulfonates, and phosphates in urine by capillary electrophoresis/mass spectrometry / Svenja-Catharina Bunz and Christian Neusüss -- Analysis of neurotransmitter amino acids by CE-LIF detection in biological fluids / Angelo Zinellu [and others] -- Expanded newborn screening of inborn errors of metabolism by capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) / Philip Britz-McKibbin -- Development of an on-site measurement system for salivary stress-related substances based on microchip CE / Yoshihide Tanaka and Nahoko Naruishi -- Simultaneous determination of atenolol and amiloride by capillary electrophoresis with capacitively coupled contactless conductivity detection (C4D) / Khaldun M. AL Azzam and Hassan Y. Aboul-Enein -- Homogeneous immunoassay of thyroxine based on microchip electrophoresis and chemiluminescence detection / Shulin Zhao and Yi-Ming Liu -- High-throughput profiling of the serum n-glycome on capillary electrophoresis microfluidics systems / Dieter Vanderschaeghe, Andras Guttman, and Nico Callewaert -- Screening of matrix metalloproteinase inhibitors by microanalysis with fluorescence detection / Xin Hai, Erwin Adams, and Ann Van Schepdael -- ABO genotyping by capillary electrophoresis / James Chun-I Lee [and others] -- Separation of hemoglobin variants by capillary electrophoresis / Frédéric Cotton and Béatrice Gulbis -- Newborn screening for hemoglobinopathies using capillary electrophoresis / P.C. Giordano -- Application of CZE in the differentiation of Staphylococcus aureus strains / Bogusław Buszewski, Katarzyna Hrynkiewicz, and Ewelina Dziubakiewicz -- Multiplex and quantitative pathogen detection with high-resolution capillary electrophoresis-based single-strand conformation polymorphism / Hee Sung Hwang [and others] -- Application of CGE to virus identification / Julia A. Fruetel and Victoria A. VanderNoot -- Capillary electrophoresis-based proteomic techniques for biomarker discovery / Xueping Fang, Chenchen Wang, and Cheng S. Lee -- Integrated affinity and electrophoresis systems for multiplexed biomarker analysis / Pamela N. Nge [and others] -- Urinary proteomics based on capillary electrophoresis coupled to mass spectrometry in kidney disease / Amaya Albalat [and others] -- Immunoaffinity, capillary electrophoresis, and statistics for studying intact alpha 1-acid glycoprotein isoforms as an atherothrombosis biomarker / Angel Puerta [and others] -- Chip-based immunoassays / Akwasi A. Apori and Amy E. Herr -- CE analysis of g-globulin mobility and potential clinical utility / Dieter Vanderschaeghe [and others] -- Cytokine analysis by immunoaffinity capillary electrophoresis / Mark Mendonca and Heather Kalish -- Capillary electrophoresis for the detection of fragile X expanded alleles / Rong Mao, Pinar Bayrak-Toydemir, and Elaine Lyon -- Analysis of microsatellite instability by microfluidic-based electrophoresis / Natalia Elfimova, Wafa Amer, and Margarete Odenthal -- HLA DR-DQ genotyping by capillary electrophoresis for risk assessment for celiac disease / Ewa H. Lavant and Joyce Carlson.
  • 2013 ClinicalKey
    Cowan, Robert A.; Gaw, Allan; Murphy, Michael J.; O'Reilly, Denis St. J.; Srivastava, Rajeev.
    The clinical biochemistry laboratory -- The use of the laboratory -- The interpretation of results -- Point of care testing -- Reference intervals -- Fluid and electrolyte balance : Concepts and vocabulary -- Water and sodium balance -- Hyponatraemia : pathophysiology -- Hyponatraemia : assessment and management -- Hypernatraemia -- Hyperkalaemia -- Hypokalaemia -- Intravenous fluid therapy -- Investigation of renal function (1) -- Investigation of renal function (2) -- Urinalysis -- Proteinuria -- Acute renal failure -- Chronic renal failure -- Acid-base : concepts and vocabulary -- Metabolic acid-base disorders -- Respiratory and mixed acid-base disorders -- Oxygen transport -- Acid-base disorders : diagnosis and management -- Proteins and enzymes -- Immunoglobulins -- Myocardial infarction -- Liver function tests -- Jaundice -- Liver disease -- Glucose metabolism and diabetes mellitus -- Diagnosis and monitoring of diabetes mellitus -- Diabetic ketoacidosis -- Hypoglycaemia -- Calcium regulation and hypocalcaemia -- Hypercalcaemia -- Phosphate and magnesium -- Bone disease -- Osteoporosis -- Endocrine control -- Dynamic function tests -- Pituitary function -- Growth disorders and acromegaly -- Thyroid pathophysiology -- Hypothyroidism -- Hyperthyroidism -- Adrenocortical pathophysiology -- Hypofunction of the adrenal cortex -- Hyperfunction of the adrenal cortex -- Gonadal function -- Subfertility -- Nutritional assessment -- Nutritional support -- Parenteral nutrition -- The metabolic response to injury -- Gastrointestinal disorders -- Iron -- Zinc and copper -- Therapeutic drug monitoring -- Toxicology -- Metal poisoning -- Alcohol -- Coma -- Ascites and pleural fluid -- Cerebrospinal and other body fluids -- Lipoprotein metabolism -- Clinical disorders of lipid metabolism -- Hypertension -- Cancer and its consequences -- Tumour markers -- Multiple endocrine neoplasia -- Hyperuricaemia -- Myopathy -- Biochemistry in the elderly -- Fetal monitoring and prenatal diagnosis -- Pregnancy -- Antenatal screening -- Screening the newborn for disease -- Paediatric biochemistry -- Inborn errors of metabolism -- Selected inherited disorders -- Case history comments -- Web resources.
  • 2015 ClinicalKey
    Ballantyne, Christie M.
    Human plasma lipoprotein metabolism -- Regulation and clearance of apolipoprotein B-containing lipoproteins -- Absorption and excretion of intestinal cholesterol and other sterols -- High-density lipoprotein metabolism -- Lipoproteins : mechanisms for atherogenesis and progression of atherothrombotic disease -- Impact of rare and common genetic variants on lipoprotein metabolism -- Lipoprotein oxidation : mechanisms and biotheranostic applications -- Cholesterol : concentration, ratio, and particle number -- High-density lipoprotein cholesterol and triglycerides in coronary heart disease risk assessment -- Lipoprotein(a) -- Clinical evaluation for genetic and secondary causes of dyslipidemia -- Use of high-sensitivity c-reactive protein for risk assessment -- Role of lipoprotein-associated phospholipase A2 in vascular disease -- Emerging assays -- Imaging atherosclerosis for risk stratification by cardiac computed tomography or carotid ultrasound -- Overview of general approach to management of elevated low-density lipoprotein cholesterol and mixed dyslipidemia, high triglycerides, and low high-density lipoprotein cholesterol -- Treatment guidelines overview -- Dietary patterns for the prevention and treatment of cardiovascular disease -- Exercise and lipids -- Weight loss -- Statins -- Bile acid sequestrants -- Cholesterol absorption inhibitors -- Niacin (nicotinic acid) -- Fibrates -- Omega-3 fatty acids -- Combination therapy for dyslipidemia -- Nutraceuticals and functional foods for cholesterol reduction -- Evolving targets of therapy : cholesteryl ester transfer protein inhibition -- Evolving targets of therapy : proprotein convertase subtilisin/kexin 9 inhibition -- Evolving targets of therapy : inflammation as a method to predict and prevent cardiovascular events -- Invasive imaging modalities and atherosclerosis : intravascular ultrasound -- Noninvasive imaging modalities and atherosclerosis : the role of ultrasound -- Noninvasive imaging modalities and atherosclerosis : the role of magnetic resonance imaging and positron emission tomography imaging -- Special patient populations : diabetes and metabolic syndrome -- Special patient populations : women and elderly -- Special patient populations : children and adolescents -- Special patient populations : familial hypercholesterolemia and other severe hypercholesterolemias -- Special patient populations : acute coronary syndromes -- Special patient populations : transplant recipients -- Special patient populations : chronic kidney disease -- Special patient populations : lipid abnormalities in high-risk ethnic groups -- Special patient populations : human immunodeficiency virus patients -- Therapeutic targeting of high-density lipoprotein metabolism.
  • 2007
    Glew, Robert H.; Rosenthal, Miriam D.
    Status: Not Checked OutLane Catalog Record
  • 2011
    Tan, Fraser Elisabeth; Brown, Patrick O.; Krasnow, Mark; Stearns, Tim; Straight, Aaron.
    In the mammalian lung, multiciliated cells lining the conducting airways play a crucial role in mucociliary clearance, the primary defense mechanism of the lung against foreign particles and bacterial infection. The cilia of the multiciliated cells beat in metachronic waves to propel mucus up from the deep distal branches of the lung and out of the trachea, to be either expectorated or swallowed. Breakdown of the mucociliary escalator, due to ciliary dysfunction as occurs in primary ciliary dyskinesia, or due to changes in osmotic balance, as occurs in cystic fibrosis, result in chronic airway bacterial infections. Viral infection in healthy individuals also causes loss of cilia, and this correlates with high rates of bacterial infections that follow many viral infections. Despite the importance of these cells, we know very little about how they are specified during development, nor about how multiple cilia are generated. Each cilium is a membrane encased microtubule based structure that protrudes from the apical surface of the cell. Most cells bear a solitary, or primary, cilium, while only a few specialized cell types can generate hundreds of cilia each. Each cilia is nucleated by a single centriole, and centriole number is normally tightly regulated. How these multiciliated cells circumvent this regulation to generate hundreds of centrioles is unknown. One the developing multiciliated cell has generated its centrioles, those centrioles migrate to and dock with the apical membrane and generate their ciliary axonemes. While many proteins involved in ciliary function and intraflagellar transport have been identified, little is known about how these process are controlled from a molecular level. Very few transcription factors are known to play a role in ciliogenesis. The most well-studied one is Foxj1, which is required for motility in single cilia, and is also critical for proper centriole migration in multiciliated cells. However, ectopic expression of Foxj1 cannot confer a ciliated fate on a non-ciliated cell, and cells null for foxj1 still generate centrioles, so there must be other transcription factors that initiate ciliogenesis and act in the early steps of the process, such as centriole generation. Here, we have identified the first transcription factor that acts upstream of Foxj1 during ciliogenesis. We screened through a subset of the known and predicted transcription factors in the mouse genome for those expressed in the developing lung in a pattern similar to that of Foxj1. We isolated a single candidate, the myeloblastosis oncogene (c-myb). We showed that c-Myb was expressed in post-mitotic cells in the developing lung epithelium, many of which co-expressed Foxj1, indicating that c-Myb is expressed in ciliating cells. Analysis of the timing of c-Myb expression during development as compared to Foxj1 and to maturing ciliary axonemes revealed that c-Myb was expressed during the early steps of ciliogenesis and was downregulated as the cells matured. Specific removal of c-Myb from the developing airway epithelium abrogated Foxj1 expression and centriole generation at E15.5. c-myb null epithelia did not have mature ciliated cells at E17.5, although Foxj1 expression and centriole generation appear to have recovered by this time point. This is the first identification of a transcription factor that regulates the initiation of ciliogenesis in the developing mouse lung. Finally, we propose a modular model of transcriptional control of cilia, in which cells deploy specific transcriptional modules to build the particular kind of cilia they require.
  • 2013 Thieme Book
    Koolman, Jan; Röhm, Klaus-Heinrich.
    Basics -- Chemistry -- Physical Chemistry -- Biomolecules -- Carbohydrates -- Lipids -- Amino acids -- Peptides and proteins -- Nucleotides and nucleic acids -- Metabolism -- Enzymes -- Metabolic pathways -- Energy metabolism -- Carbohydrate metabolism -- Lipid metabolism -- Protein metabolism -- Nucleotide metabolism -- Porphyrin metabolism -- Cell organelles -- Basics -- Cytoskeleton -- Nucleus -- Mitochondria -- Membranes -- ER and Golgi apparatus -- Lysosomes -- Peroxisomes -- Molecular Genetics -- Overview -- Genome -- Chromatin -- Gennetic code -- Genetic engineering -- Tissues and organs -- Digestive system -- Blood -- Immune system -- Liver -- Adipose tissue -- Kidney -- Muscle -- Connective tissue -- Brain and sensory organs -- Integration of metabolism -- Nutrition -- Nutrients -- Vitamins -- Signaling systems -- Signal transduction -- Hormone systems -- Lipophilic signaling substances -- Hydrophilic signaling substances -- Growth and development -- Appendix.
  • 2006 CRCnetBASE
    Boldi, Armen M.
  • v. 1-7, 1960-1964.
    Florkin, Marcel; Mason, Howard S.
    Status: Not Checked OutLane Catalog Record
    v. 1. Sources of free energy -- v. 2. Free energy and biological function -- v. 3-5. Constituents of life -- v. 6. Cells and organisms -- v. 7. Supplementary volume. [and Topical subject index, volumes I-VII]
  • ScienceDirect
    Ginsburg, Victor.
    Also available: Print – 1982
  • 2009 ScienceDirect
    Hopwood, D. A.
    Also available: Print – 2009
  • 2009 ScienceDirect
    Hopwood, D. A.
    Also available: Print – 2009
  • 2015 CRCnetBASE
    Burkowski, Forbes J.
    Chapter 1. Introduction : macromolecules and chimera -- chapter 2. Accessing and displaying molecular data with chimera -- chapter 3. Algorithms dealing with distance -- chapter 4. Algorithms dealing with angles -- chapter 5. Structure overlap and alignment -- chapter 6. Potential energy functions -- chaper 7. Rotamers and side-chain conformation -- chapter 8. Residue networks.
  • 2015 ScienceDirect
    Burke, Donald H.; Chen, Shi-Jie.
    This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers computational prediction RNA structure and dynamics, including such topics as computational modeling of RNA secondary and tertiary structures, riboswitch dynamics, and ion-RNA, ligand-RNA and DNA-RNA interactions.Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers computational methods and applications in RNA structure and dynamics.
  • 2015 Springer Protocols
    Huang, Jian; Zhou, Peng.
    De novo peptide structure prediction : an overview / Pierre Thévenet [and three others] -- Molecular modeling of peptides / Krzysztof Kuczera -- Improved methods for classification, prediction, and design of antimicrobial peptides / Guangshun Wang -- Building MHC class II epitope predictor using machine learning approaches / Loan Ping Eng, Tin Wee Tan, and Joo Chuan Tong -- Brownian dynamics simulation of peptides with the University of Houston Brownian Dynamics (UHBD) program / Tongye Shen and Chung F. Wong -- Computational prediction of short linear motifs from protein sequences / Richard J. Edwards and Nicolas Palopoli -- Peptide toxicity prediction / Sudheer Gupta [and five others] -- Synthetic and structural routes for the rational conversion of peptides into small molecules / Pasqualina Liana Scognamiglio, Giancarlo Morelli, and Daniela Marasco -- In silico design of antimicrobial peptides / Giuseppe Maccari, Mariagrazia Di Luca, and Riccardo Nifosì -- Information-driven modeling of protein-peptide complexes / Mikael Trellet, Adrien S.J. Melquiond, and Alexandre M.J.J. Bonvin -- Computational approaches to developing short cyclic peptide modulators of protein-protein interactions / Fergal J. Duffy, Marc Devocelle, and Denis C. Shields -- A use of homology modeling and molecular docking methods : to explore binding mechanisms of nonylphenol and bisphenol A with antioxidant enzymes / Mannu Jayakanthan [and three others] -- Computational peptide vaccinology / Johannes Söllner -- Computational modeling of peptide-aptamer binding / Kristen L. Rhinehardt, Ram V. Mohan, and Goundla Srinivas.
  • 2012 Springer
    Karthik, M. V. K.; Shukla, Pratyoosh.
  • 2014 ScienceDirect
    Galluzzi, Lorenzo; Kroemer, Guido.
    Metabolic alterations of cancer cells / Marco Sciacovelli, Edoardo Gaude, Mika Hilvo, and Christian Frezza -- Autophagy and cancer metabolism / Juliet Goldsmith, Beth Levine, and Jayanta Debnath -- Regulation of cancer metabolism by oncogenes and tumor suppressors / Raffaella Iurlaro, Clara Lucía León-Annicchiarico, and Cristina Muñoz-Pinedo -- Cross talk between cell death regulation and metabolism / Simone Fulda -- Techniques to monitor glycolysis / Tara TeSlaa and Michael A. Teitell -- Measurement of enolase activity in cell lysates / Keigo Fukano and Kazuhiro Kimura / Extracellular flux analysis to monitor glycolytic rates and mitochondrial oxygen consumption / Martin Pelletier, Leah K. Billingham, Madhu Ramaswamy, and Richard M. Siegel -- Conventional techniques to monitor mitochondrial oxygen consumption / Hélène Simonnet, Arnaud Vigneron, and Jacques Pouysségur -- Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry / Gerhard Krumschnabel, Andrea Eigentler, Mario Fasching, and Erich Gnaiger -- Kinetic analysis of local oxygenation and respiratory responses of mammalian cells using intracellular oxygen-sensitive probes and time-resolved fluorometry / Alexander V. Zhdanov, Ruslan I. Dmietriev, James Hynes, and Dmitri B. Papkovsky -- Cell-based measurements of mitochondrial function in human subjects / Ju-Gyeong Kang, Ping-yuan Wang, and Paul M. Hwang -- Use of chemical probes to detect mitochondrial ROS by flow cytometry and spectrofluorometry / Jing Chen and Clayton E. Mathews -- Methods to monitor ROS production by fluorescence microscopy and fluorometry / Aleksandra Wojtala, Massimo Bonora, Dominika Malinska, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski -- Genetically encoded redox sensor / Wai Kan Chiu, Atif Towheed, and Michael J. Palladino -- Use of genetically encoded sensors to monitor cytosolic ATP/ADP ratio in living cells / Andrei I. Tarasov and Guy A. Rutter -- Methods to monitor and compare mitochondrial and glycolytic ATP production / Simone Patergnani, Federica Baldassari, Elena De Marchi, Agnieszka Karkucinska-Wieckowska, Mariusz R. Wieckowski, and Paolo Pinton -- Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption / Christos Chinopoulos, Gergely Kiss, Hibiki Kawamata, and Anatoly A. Starkov -- Real-time assessment of the metabolic profile of living cells with genetically encoded NADH sensors / Yuzheng Zhao, Yi Yang, and Joseph Loscalzo -- 13C isotope-assisted methods for quantifying glutamine metabolism in cancer cells / Jie Zhang, Woo Suk Ahn, Paulo A. Gameiro, Mark A. Keibler, Zhe Zhang, and Gregory Stephanopoulos -- Measurement of fatty acid oxidation rates in animal tissues and cell lines / Frank K. Huynh, Michelle F. Green, Timothy R. Koves, and Matthew D. Hirschey -- Methods to assess lipid accumulation in cancer cells / Jørgen Sikkeland, Yang Jin, and Fahri Saatcioglu -- Analysis of hypoxia-induced metabolic preprogramming / Chendong Yang, Lei Jiang, Huafeng Zhang, Larissa A. Shimoda, Ralph J. Bernardinis, and Gregg L. Semenza.
  • 2010 Springer
    Groettrup, Marcus.
    To cope with the steadily growing body of knowledge and for organizational reasons the articles in this book are focussed on the modifiers ubiquitin, SUMO1/2/3, NED 8, ISG15 and FAT10 but it should be at least mentioned here that enzymes involved in conjugation and deconjugation of URM1, UFM1, ATG8 and ATG12 have been reported and continue to be investigated.
  • pt. A-B, 2010. ScienceDirect
    pt. B ScienceDirect
    Conn, P. Michael.
    Also available: Print – pt. A-B, 2010.
  • 2009 CRCnetBASE
    Floris, Giovanni; Mondovì, Bruno.
    History / Franca Buffoni -- Nomenclature and potential functions of copper amine oxidases / Sinéad Boyce ... [et al.] -- Cofactors of amine oxidases : copper ion and its substitution and the 2,4,5-trihydroxylphenylalanine quinone / Shinnichiro Suzuki ... [et al.] -- Copper amine oxidases from plants / Rosaria Medda ... [et al.] -- Soluble copper amine oxidases from mammals / Paola Pietrangeli ... [et al.] -- Membrane-bound copper amine oxidases / Andrew Holt -- Copper amine oxidase genes / Ivo Frébort and Hubert G. Schwelberger -- Mechanism of TPQ biogenesis in prokaryotic copper amine oxidase / Toshihide Okajima and Katsuyuki Tanizawa -- Copper amine oxidase crystal structures / J. Mitchell Guss, Giuseppe Zanotti, and Tiina A. Salminen -- Plasma amine oxidases in various clinical conditions and in apoptosis / Frans Boomsma, Anton H. van den Meiracker, and Antonio Toninello -- Copper amine oxidases in intestinal diseases / Wieslawa Agnieszka Fogel, Ewa Toporowska-Kowalska, and Anna Stasiak -- Copper amine oxidases in adipose tissue-related disorders / Christian Carpéné -- Copper amine oxidases in adhesion molecules in renal pathology and in Alzheimer's disease and VAP-1 in leukocyte migration / Peter Boor ... [et al.] -- Inhibitors of copper amine oxidases : past, present, and future / Marek Šebela and Lawrence M. Sayre -- Pharmacological applications of copper amine oxidases / Emanuela Masini and Laura Raimondi -- Copper amine oxidases as antioxidant and cardioprotective agents / Mircea Alexandru Mateescu and Réginald Nadeau -- Biotechnological aspects of copper amine oxidases / Roberto Stevanato -- Concluding remarks / David M. Dooley.
  • 2008 Springer
    Clemen, Christoph S.; Eichinger, Ludwig; Rybakin, Vasily.
    Phylogenetic, structural, and functional relationships between WD and kelch repeat proteins / Andrew M. Hudson and Lynn Cooley -- Diversity of WD repeat proteins / Temple F. Smith -- A brief history of the coronin family / Eugenio L. de Hostos -- Molecular phylogeny and evolution of the coronin gene family / Reginald O. Morgan and M. Pilar Fernandez -- Coronin structure and implications / Bernadette McArdle and Andreas Hofmann -- Coronin : the double-edged sword of actin dynamics / Meghal Gandhi and Bruce L. Goode -- Invertebrate coronins / Maria C. Shina and Angelika A. Noegel -- Evolutionary and functional diversity of coronin proteins / Charles Peter Xavier ... [et al.] -- Role of mammalian coronin 7 in the biosynthetic pathway / Vasily Rybaki -- Coronin 1 in innate immunity / Jean Pieters -- The role of mammalian coronins in development and disease / David W. Roadcap, Christoph S. Clemen, and James E. Bear.
  • 2007 Springer
    Salomons, Gajja S.; Wyss, Markus.
  • 2010- Wiley
    Current Protocols in Chemical Biology provides validated protocols and overviews about specialized chemical techniques and tools for studies of biology and drug design. These tools include small-molecule design, synthesis, derivatization, handling, and detection as well as advances in laboratory automation, robotics, and medicinal chemistry as applied to high-throughput screening (HTS). Methods for modification of proteins, nucleic acids, carbohydrates and lipids for their use as tools in the study of particular biological systems are also included
  • 2002- Wiley
    Beaucage, Serge L.
  • 2001- Wiley
    Enna, S. J.
  • [1995]- Wiley
    Coligan, John E.
    Also available: Print – 1995-2005.
  • 2008 Springer Protocols
    Ayoub, Samir S.; Flower, R. J.; Seed, Michael P.
    Part I. In vitro protocols for studying expression and activity of cyclooxygenase enzymes and characterisation of their inhibition patterns -- 1. In vitro cyclo-oxygenase expression and activity protocols : introduction to Part I / Roderick J. Flower -- 2. Techniques used to study regulation of cyclooxygenase-2 promoter sites / Hiroyasu Inoue and Rieko Nakata -- 3. Purification of recombinant human COX-1 and COX-2 / James K. Gierse -- 4. Cloning and expression of cyclooxygenase-1 and cyclooxygenase-2 / Nicholas R. Staten and Beverly A. Reitz -- 5. Expression of cyclooxygenase isoforms in the baculovirus expression system / John C. Hunter, Natalie M. Myres, and Daniel L. Simmons -- 6. Peroxidase active site activity assay / Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett -- 7. Study of inhibitors of the PGH synthases for which potency Is regulated by the redox state of the enzymes / Olivier Boutaud and John A. Oates -- 8. Different methods for testing potential cyclooxygenase-1 and cyclooxygenase-2 inhibitors / Stefan Laufer and Sabine Luik -- 9. In vitro cyclooxygenase activity assay in tissue homogenates / Samir S. Ayoub -- 10. Techniques used to characterize the binding of cyclooxygenase Inhibitors to the cyclooxygenase active site / William F. Hood -- 11. In vitro Assays for cyclooxygenase activity and inhibitor characterization / Mark C. Walker and James K. Gierse -- -- Part II. Extraction and measurement of prostanoids and isoprostanes -- 12. Extraction and measurement of prostanoids and isoprostanes : introduction to Part II / Paola Patrignani -- 13. Prostanoid extraction and measurement / Lorenzo Polenzani and Samir S. Ayoub -- 14. Measurement of 8-iso-prostaglandin F2a in biological fluids as a measure of lipid peroxidation / Stefania Tacconelli, Marta L. Capone, and Paola Patrignani -- -- Part III. In vivo models to study involvement of cyclooxygenase products in health and disease -- 15. In vivo models to study cyclooxygenase products in health and disease: introduction to Part III / Derek W. Gilroy, Melanie Stables, and Justine Newson -- 16. Protocols to assess the gastrointestinal side effects resulting from inhibition of Cyclo-Oxygenase isoforms / Brendan J.R. Whittle -- 17. Cyclooxygenase enzymes and their products in the carrageenan-induced pleurisy in rats / Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed -- 18. Iloprost-induced nociception : determination of the site of anti-nociceptive action of cyclooxygenase inhibitors and the Involvement of cyclooxygenase products in central mechanisms of nociception / Samir S. Ayoub and Regina M. Botting.
  • 2015 Springer
    Ortiz de Montellano, Paul R.
    This authoritative Fourth Edition summarizes the advances of the past decade concerning the structure, mechanism, and biochemistry of cytochrome P450 enzymes, with sufficient coverage of earlier work to make each chapter a comprehensive review of the field. Thirteen chapters are divided into two detailed volumes, the first covering the fundamentals of cytochrome P450 biochemistry, as well as the microbial, plant, and insect systems, and the second exclusively focusing on mammalian systems. Volume 1 begins with an exploration of the biophysics and mechanistic enzymology of cytochrome P450 enzymes, with a discussion of the structures of P450 enzymes and their electron donor partners, the mechanisms of oxygen activation and substrate oxidation, and the approaches and nature of cytochrome P450 inhibition. Two more chapters discuss the nature and roles of cytochrome P450 enzymes in microbes, plants and insects, and an eighth chapter is a survey of the potential utility of P450 enzymes in biotechnology. The first chapter of Volume 2 examines the roles of P450 enzymes in mammals, mainly humans. Four further chapters then deal with the genetic and hormonal regulation of P450 enzymes and their specific roles in the processing of sterols and lipids. Cytochrome P450: Structure, Mechanism, and Biochemistry is a key resource for scientists, professors, and students interested in fields as diverse as biochemistry, chemistry, biophysics, molecular biology, pharmacology and toxicology.
  • 2014 Springer
    Dey, Aparajita.
    The book deals with various clinical aspects of cytochrome P450 2E1 (CYP2E1) which is a potent source for oxidative stress. Oxidative stress is critical for pathogenesis of diseases and CYP2E1 is a major contributor for oxidative stress. Several clinical disorders are associated with changes in regulation of CYP2E1 and the consequent abnormalities which include alcoholic liver disease, alcoholic pancreatitis, carcinogenesis, non-alcoholic fatty liver disease, non-alcoholic steatohepatitis, obesity, hepatitis C virus infection, reproductive organ toxicity, hepatocellular and cholestatic liver cirrhosis, inhibition of bone repair, cross-tolerance in smokers and people treated with nicotine, disorders of central nervous system, changes in metabolism of protoxicants in the circulatory system and susceptibility to human papillomavirus infection. Hence, CYP2E1 emerges as a new and potent player in aggravating injury and furthering disease complications.
  • 2002 ScienceDirect
    Johnson, Eric F.; Waterman, Michael R.
    Also available: Print – 2002
  • 2013 Springer Protocols
    Ortiz de Montellano, Paul R.; Phillips, Ian R.; Shephard, Elizabeth A.
    This volume provides a wide range of techniques accessible to researchers in biochemistry, molecular biology, pharmacology, toxicology, environmental biology and genetics. It features step-by-step, readily reproducible protocols and notes on troubleshooting.
  • 2011 Springer Protocols
    Eisenacher, Martin; Hamacher, Michael; Stephan, Christian.
    Instruments and methods in proteomics -- In-depth protein characterization by mass spectrometry -- Analysis of phosphoproteomics data -- The origin and early reception of sequence databases -- Laboratory data and sample management for proteomics -- PRIDE and "Database on demand" As valuable tools for computational proteomics -- Analysing proteomics identifications in the context of functional and structural protein annotation: Integrating annotation using PICR, DAS, and BioMart -- Tranche distributed repository and proteomecommons.Org -- Data standardization by the HUPO-PSI: How has the community benefitted? -- mzIdentML: An open community-built standard format for the results of proteomics spectrum identification algorithms -- Spectra, chromatograms, metadata: mzML-the standard data format for mass spectrometer output -- imzML: Imaging mass spectrometry markup language: A common data format for mass spectrometry imaging -- Tandem mass spectrometry spectral libraries and library searching -- Inter-lab proteomics: Data mining in collaborative projects on the basis of the HUPO brain proteome project's pilot studies -- Data management and data integration in the HUPO plasma proteome project -- Statistics in experimental design, preprocessing, and analysis of proteomics data -- The evolution of protein interaction networks -- Cytoscape: Software for visualization and analysis of biological networks -- Text mining for systems modeling -- Identification of alternatively spliced transcripts using a proteomic informatics approach -- Distributions of ion series in ETD and CID spectra: Making a comparison -- Evaluation of peak-picking algorithms for protein mass spectrometry -- OpenMS and TOPP: Open source software for LC-MS data analysis -- LC/MS data processing for label-free quantitative analysis -- Spectral properties of correlation matrices - towards enhanced spectral clustering -- Standards, databases, and modeling tools in systems biology -- Modeling of cellular processes: Methods, data, and requirements.
  • 2011
    Havenstrite, Karen; Blau, Helen M.; Nolan, Garry P.; Wang, Clifford.
    The ultimate goal of regenerative medicine is to repair tissues damaged by aging, injury or disease. Tissue-specific adult stem cells constitute a reservoir of cells in postnatal tissue that have the remarkable capacity to proliferate and repair tissue damage because they can both self-renew, or produce more stem cells, and differentiate into mature cells. Adult stem cells have been identified in a variety of tissues, including blood, brain, skin, intestine, and muscle and methods exist to prospectively isolate these populations by flow cytometry. Upon transplantation, they possess an extraordinary ability to contribute extensively to tissue regeneration. Notably, adult stem cells are a relatively rare cell population and methods to propagate and expand these cell types in culture without loss of regenerative capacity are lacking, a hurdle to their clinical use. In vivo, stem cells reside in an instructive microenvironment, or niche, which serves to regulate fate decisions, including quiescence, self-renewal, and differentiation. Given the complexity of their native environment, it is not surprising that upon removal from their niche they rapidly lose regenerative capacity. While the role of biochemical signals in regulating stem cell fate and function has been widely explored, the effects of biophysical signals have not been discerned. To elucidate the role of matrix elasticity in regulating adult stem cell fate, we first design a biomimetic hydrogel culture platform to mimic tissue elasticity and physiologic presentation of biochemical cues. Adapting a previously described conjugate addition reaction, poly(ethylene glycol) hydrogel substrates are fabricated which have a Young's modulus that is tunable in the physiologic range (1-50kPa). Gels are designed to have limited post-polymerization swelling to enable constant density of tethered biological ligands. Utilizing this tunable hydrogel culture platform, we provide the first definitive evidence that matrix elasticity regulates adult stem cell self-renewal in culture. Using a combination of culture studies and in vivo functional assays in mice, we demonstrate that substrate rigidity profoundly impacts the self-renewal potential of tissue-specific adult stem cells isolated from skeletal muscle and bone marrow. In contrast with rigid tissue culture plastic in which regenerative potential is lost, we demonstrate that culture on a pliant hydrogel substrate maintains the 'stemness' of muscle stem cells (MuSCs) and hematopoetic stem cells (HSCs). Further, we describe a novel in vivo screen and identify an extracellular matrix molecule which, in conjunction with soft hydrogel, has a previously unrecognized role in regulating HSC fate. These studies demonstrate that recapitulating tissue rigidity, a key component of the in vivo microenvironment, enables propagation of functional adult stem cells in culture for the first time. We expect these experimental approaches will be broadly applicable to other adult stem cell types and ultimately will profoundly impact regenerative medicine by enabling generation of functional stem cell populations for use in clinical cell-based therapies.
  • 2010
    Clemons, Heather Jean; Brown, Patrick O.; Chu, Gilbert; Kingsley, David M.; Krasnow, Mark.
    Adipose tissue is found in diverse depots throughout the human body. The diversity of physiological specialization of these fat depots is reflected in the diverse depot-specific alterations seen in lipodystrophies and links between specific patterns of fat distribution and susceptibility to diseases, including Type II Diabetes and coronary artery disease. Previous studies, although focused on only 2 major fat depots (omental and abdominal subcutaneous), have identified numerous differences in metabolic, endocrine and developmental programs. We carried out a more extensive and higher resolution investigation of the anatomic specialization of white adipose tissue, based on 59 samples collected from 7 anatomically diverse fat depots, from 56 individuals. Using DNA microarrays we measured relative abundance of ~25,000 distinct mRNAs in each adipose tissue sample and in adipocytes and stromal-vascular cells isolated from each sample, and compared their gene expression patterns with ~120 samples representing diverse non-adipose tissues. Adipocytes from different regions of the body displayed distinct gene expression profiles. Characteristic patterns of expression of HOX genes distinguished adipocyte samples by site of origin; these patterns were recapitulated when adipocyte precursors from each site were cultured and differentiated ex vivo, suggesting that these genes may have a role in programming and/or maintaining depot-specific differentiation of adipocytes. Expression in adipocytes of 300 genes with major roles in energy metabolism showed both depot-dependent and inter-individual variation. Some of the patterns suggested important differences among anatomic depots in metabolic roles. For example, a set of genes involved in lipid uptake and storage and hormone-regulated lipolysis were generally most highly expressed in breast, pericolonic and omental adipocytes whereas genes involved in glycogen metabolism and de novo fatty acid synthesis were generally most highly expressed in breast and abdominal subcutaneous adipocytes, suggesting that these depots play a role as glucose buffers. Dozens of genes known or predicted to encode secreted molecular signals were highly expressed in adipocytes compared to either adipose stromal-vascular cells or other organs; many of these were differentially expressed among adipocyte depots. Some of these genes appear to be good candidates for encoding novel adipokines. In summary, we found extensive variation among adipose depots, as well as inter-individual variation, in global gene expression. Differences in genes linked to development, metabolism and cell-cell communication suggest new hypotheses relevant to depot-specific and general aspects of adipocyte biology.
  • 2015 Springer Protocols
    Kurien, Biji T.; Scofield, R. Hal.
    Western blotting : origin and ascent of the species / W. Neal Burnette -- Methods to concentrate proteins for protein isolation, proteomic, and peptidomic evaluation / J. P. Dean Goldring -- Measuring protein concentration on nitrocellulose and after the electrophoretic transfer of protein to nitrocellulose / J. P. Dean Goldring -- Detection of blotted proteins : not all blockers are created equal / Vishal Kothari and Suresh T. Mathews -- Protein stains to detect antigen on membranes / Anil Dsouza and R. Hal Scofield -- Fluorescent labeling of proteins and its application to SDS-PAGE and Western blotting / F. Javier Alba, Salvador Bartolomé, Antonio Bermúdez, and Joan-Ramon Daban -- Rapid, antibody-free detection of recombinant proteins on blots using enzyme fragment complementation / Neil W. Charter, Joe Horecka Chin-Yee Loh, Albert Doan, Tom Wehrman, and Keith R. Olson -- Use of nonradioactive detection method for north and South-Western blot / Claudia Franke, Daniel Gräfe, Holger Bartsch, and Michael P. Bachmann -- Immunoblotting using radiolabeled reagents for detection / Holger Bartsch, Claudia Franke, and Michael P. Bachmann -- Immunoblotting of antigens : whole, strip, and new-line nitrocellulose membrane immunoblotting using the chemiluminescence technique / Yaser Dorri -- Detection of protein carbonyls by means of biotin hydrazide-streptavidin affinity methods / Kenneth Hensley -- Direct immunodetection of antigens within the precast polyacrylamide gel / Surbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend -- Quantitative analysis of signal transduction with in-cell Western immunofluorescence assays / Vince Boveia and Amy Schutz-Geschwender -- Ultrasensitive protein detection on dot blots and Western blots with semiconducting polymer dots / Fangmao Ye, Polina B. Smith, and Daniel T. Chiu -- Co-detection of target and total protein by CyDye labeling and fluorescent ECL plex immunoblotting in a standard proteomics workflow / Caitriona Scaife, Ciara A. McManus, Pamela M. Donoghue, and Michael J. Dunn -- Using biotinylated proteins to demonstrate immunodetection of antigens via Western blotting, dot blots, and immunohistochemistry / Thomas Millar, Ronald Knighton, and Jo-Anne Chuck -- Calcium binding by Ro 60 multiple antigenic peptides on PVDF membrane / Biji T. Kurien and Michael P. Bachmann -- Sequential use of immunoblots for characterization of autoantibody specificities / Holger Bartsch and Michael P. Bachmann -- Nanogold immunodetection detection systems for the identification of autoantigens by Western blotting / Jacen S. Moore and R. Hal Scofield -- Application of intermittent microwave irradiation to Western blot analysis / Yu-Ting Liu and Shinya Toyokuni -- Visualization of unstained protein bands on PVDF / Jun Park, Masaharu Mabuchi, and Ajay Sharma -- Multiplexed fluorescent immunodetection using low autofluorescence Immobilon®-FL membrane / Jun Park, Masaharu Mabuchi, and Ajay Sharma -- Cold microwave-enabled protein detection and quantification / Niels Grützner, Romy M. Heilmann, Aaron G. Smith, Carol B. Johnson, Stanislav Vitha, Jörg M. Steiner, and Andreas K. Holzenburg -- TLC-blot (Far-Eastern blot) and its application to functional lipidomics / Takao Taki -- Analysis of electroblotted proteins by mass spectrometry / Jose L. Luque-Garcia and Thomas A. Neubert -- On-membrane renaturation of recombinant Ro60 autoantigen by calcium ions / Biji T. Kurien and Michael P. Bachmann -- Phosphoprotein detection on protein electroblot using a phosphate-specific fluorophore / Lee Broderick Bockus and R. Hal Scofield -- Purification of tryptic digests on polyvinylidene difluoride membrane / Biji T. Kurien and R. Hal Scofield -- Detection of blotted proteins on nitrocellulose/PVDF membranes by Alta / Jayanta K. Pal, Shilpa J. Rao, and Dhanashri J. Godbole -- Nonstripping "rainbow" and multiple antigen detection (MAD) Western blotting / Stan (Stanislaw) Krajewski, Michelle M. Tsukamoto, Xianshu Huang, and Sebastian B. Krajewski -- Supported molecular matrix electrophoresis / Yu-ki Matsuno and Akihiko Kameyama -- Parafilm-M®, an available cost-effective alternative for immuno-blot pouches / Syed M. S. Quadri -- Succinylation-alcian blue staining of mucins on polyvinylidene difluoride membranes / Akihiko Kameyama, Weijie Dong, and Yu-ki Matsuno -- Comparison of chemiluminescence vs. infrared techniques for detection of fetuin-A in saliva / Suresh T. Mathews, Emily Graff, Robert L. Judd, and Vishal Kothari -- A novel methodology for stripping and reprobing of Western blots originally developed with colorimetric substrate TMB / Parmita Kar, Saurabh Kumar Agnihotri, Archana Sharma, Rekha Sachan, Madan Lal Bhatt, and Monika Sachdev -- Other notable methods of membrane protein detection : a brief review / Biji T. Kurien and R. Hal Scofield -- Nitrocellulose membrane : the new canvas / Jasmin R. Kurien and Bianca A. Kurien -- Invisible ink marking in ECL membrane assays / Biji T. Kurien.
  • 2011 CRCnetBASE
    Lundblad, Roger L.
    "A comprehensive assessment of biomarkers, this book covers the history and current status of the application of biomarkers in diagnostics and prognostics. It explores the technology used for the study of biomarkers, and the validation of biomarkers including a comparison of the various technologies used to identify and measure biomarkers. The editors emphasize the technology underlying biomarkers and the translation of basic science to clinical laboratory technology, including the commercial development of biomarkers. The book also covers proteomics and proteomic technologies and their applications in the identification of biomarkers"--Provided by publisher.
  • 2010
    Franzini, Raphael Marcel; Du Bois, Justin; Huestis, Wray H.; Kool, Eric T.
    Templated fluorescence activation is an elegant nucleic acid detection technique, which relies on a target-activated chemical reaction between two oligonucleotide probes, eliciting a fluorescence readout. This method reports on genetic markers in solution phase and in cells. The research described in this thesis was aimed to expand the reaction scope of templated fluorescence activation and to develop probes, which overcome shortcomings of previous designs. Examples of DNA template-mediated fluorogenic reactions developed during this project include an organomercury-induced cyclization of Rhodamine thiosemicarbazide and the deprotection of a 7-azidomethoxycoumarin profluorophore by a Staudinger reaction. The most promising probe design (Q-STAR probes) is based on the reductive cleavage of an [alpha]-azidoether quencher release linker conjugated to a fluorophored-labeled DNA. A triphenylphosphine DNA-probe rapidly activates Q-STAR probes in the presence of the matched DNA target strand, reporting its presence by a strong fluorescence turn-on signal. Q-STAR probes are inert to aqueous conditions and cellular components, properties that were suboptimal for previous probe designs. Q-STAR probes report the target with single nucleotide specificity and enable an amplified detection signal by harnessing the target as a catalyst for the templated reaction. Q-STAR probes efficiently detect the presence of rRNAs in bacteria and mammalian cells. The probes are responsive to single nucleotide differences, which allows discriminating bacteria species by genetic variations using fluorescence microscopy or flow cytometry. Similarly, rRNAs in mammalian cells generate a strong fluorescence turn-on signal for Q-STAR probes. Templated fluorescence activation schemes bear considerable promise for applications in clinical diagnostics and molecular biology. The probe designs described in this thesis, in particular Q-STAR probes, constitute a major advancement in the field and will help achieve these goals.
  • 2014 ClinicalKey
    Dabbs, David J.
    Diagnostic Immunohistochemistry presents the latest information and most reliable guidance on immunohistological diagnoses in surgical pathology. David J. Dabbs, MD and other leading experts bring you state-of-the-art coverage on genomic and theranostic applications, molecular anatomic pathology, immunocytology, Non-Hodgkin's lymphoma, and more. Additional features such as tables discussing antibody specifications, differential diagnosis boxes, ancillary anatomic molecular diagnostics, and full-color histological images ensure user-friendly coverage that makes key information easy to find and apply. The fully searchable text is also available online at expertconsult.com, along with a downloadable image bank and access to Path Consult. This concise and complete resource is today's indispensable guide to the effective use of immunohistochemical diagnosis. Discusses diagnostic pitfalls through immunohistologic differential diagnosis wherever appropriate so you can provide the most accurate diagnoses. Presents chapters arranged by organ system for comprehensive coverage of all relevant information in a convenient and intuitive organization. Provides quick reference graphs for antibodies throughout the text that illustrate the frequency of immunostaining for a variety of antibodies in tumors. Includes Key Diagnostic Points boxes in every chapter for a quick summary of text areas that are of particular importance. Features an expert author for each chapter to ensure coverage of the current state of the art.
  • Knovel
    Stenesh, J.
    Also available: Print – 1989
  • 2012 Springer Protocols
    Cramer, Rainer; Westermeier, Reiner.
    Part I Fundamentals -- DIGE : past and future / Jonathan S. Minden -- Basics of 2D DIGE / Phil Beckett -- Multifluorescence 2D gel imaging and image analysis / Ingo Vormbrock, Sonja Hartwig, and Stefan Lehr -- Assessing signal-to-noise in quantitative proteomics : multivariate statistical analysis in DIGE experiments / David B. Friedman -- Analysis of proteins using DIGE and MALDI mass spectrometry / Witold M. Winnik [and others] -- Synthesis and validation of cyanine-based dyes for DIGE / Michael E. Jung [and others] -- Part II Methods -- 2D DIGE saturation labeling for minute sample amounts / Georg J. Arnold and Thomas Frohlich -- Proteomic analysis of redox-dependent changes using cysteine-labeling 2D DIGE / Hong-Lin Chan, John Sinclair, and John F. Timms -- Analysis of protein posttranslational modifications using DIGE-based proteomics / Robert M. DeKroon [and others] -- Comparative analyses of protein complexes by blue native DIGE / Katrin Peters and Hans-Peter Braun -- 2D DIGE analysis of protein extracts from muscle tissue / Cecilia Gelfi and Sara De Palma -- Combination of highly efficient hexapeptide ligand library-based sample preparation with 2D DIGE for the analysis of the hidden human serum/plasma proteome / Sonja Hartwig and Stefan Lehr -- 2D DIGE analysis of serum after fractionation by proteominertm beads / Cynthia Liang, Gek San Tan, and Maxey C.M. Chung -- Study design in DIGE-based biomarker discovery / Alexandra Graf and Rudolf Oehler -- Comparative 2D DIGE analysis of the depleted serum proteome for biomarker discovery / Megan Penno, Matthias Ernst, and Peter Hoffmann -- Part III Applications in clinical proteomics -- Differential gel-based proteomic approach for cancer biomarker discovery using human plasma / Keun Na [and others] -- 2D DIGE for the analysis of RAMOS cells subproteomes / Marisol Fernandez and Juan Pablo Albar -- Application of saturation labeling in lung cancer proteomics / Gereon Poschmann [and others] -- Proteomic profiling of the epithelial-mesenchymal transition using 2D DIGE / Rommel A. Mathias, Hong Ji, and Richard J. Simpson -- Method for protein subfractionation of cardiovascular tissues before DIGE analysis / Athanasios Didangelos, Xiaoke Yin, and Manual Mayr -- Application of DIGE and mass spectrometry in the study of type 2 diabetes mellitus mouse models / Celia Smith, Davinia Mills, and Rainer Cramer -- Evaluating the efficacy of subcellular fractionation of blast cells using live cell labeling and 2D DIGE / Yin Ying Ho [and others] -- Part IV Applications in animal, plant, and microbial proteomics -- DIGE analysis of plant tissue proteomes using a phenolic protein extraction method / Christina Rode [and others] -- Native DIGE of fluorescent plant protein complexes / Veronika Reisinger and Lutz Andreas Eichacker -- Overview of 2D DIGE analysis of marine (environmental) bacteria / Ralf Rabus -- Application of 2D DIGE in animal proteomics / Ingrid Miller.
  • 2006 Springer
    Bank, Ute; Lendeckel, Uwe; Reinhold, Dirk.
    Topic I. Structure and function. Peptide substrates of dipeptidyl peptidases / Inger Brandt ... [et al.] Phosphorus-containing inhibitors of proteolytic enzymes / Angel Stöckel-Maschek ... [et al.] Biochemical properties of recombinant prolyl dipeptidases DPP-IV and DPP8 / Xin Chen. Prediction of dipeptidyl peptidase (DP) 8 structure by homology modelling / Melissa R. Pitman, R. Ian Menz, and Catherine A. Abbott -- Topic II. DPIV-related enzymes. Structure and function in dipeptidyl peptidase IV and related proteins / Mark D. Gorrell ... [et al.] Expression and function of dipeptidyl peptidase IV and related enzymes in cancer / Petr Busek ... [et al.] DP8 and DP9 have extra-enzymatic roles in cell adhesion, migration, and apoptosis / Denise M.T. Yu ... [et al.] In vivo effects of a potent, selective DPPII inhibitor: UAMC00039 is a possible tool for the elucidation of the physiological function of DPPII / Marie-Berthe Maes ... [et al.] Expression of dipeptidyl peptidase IV-like enzymes in human peripheral blood mononuclear cells / Sabine Wrenger ... [et al.] Dipeptidyl peptidase 8 has post-proline dipeptidyl aminopeptidase and prolyl endopeptidase activities / Joohong Park ... [et al.] Prolyl endopeptidase cleaves the apoptosis rescue peptide humanin and exhibits and unknown post-cysteine cleavage specificity / Joachim Wolfgang Bär ... [et al.] Distribution of dipeptidyl peptidase IV-like activity enzymes in canine and porcine tissue sections by RT-PCR / Leona Wagner ... [et al.] -- Topic III. Metabolic disorders. Relative contribution of incretins to the glucose lowering effect of DP IV inhibitors in type 2 diabetes mellitus (T2DM) / Simon A. Hinke, Raymond A. Pederson, and Christopher H.S. McIntosh. Dipeptidyl peptidase IV: a molecular switch of vascular actions of neuropeptide Y / Lijun Li, Hans-Ulrich Demuth, and Zofia Zukowska -- Topic IV. Immune disorders. Dipeptidylpeptidase IV (DPIV) and alanyl-aminopeptidases (AAPs) as a new target complex for treatment of autoimmune and inflammatory diseases: proof of concept in a mouse model of colitis / Ute Bank ... [et al.] Dipeptidyl peptidases and inflammatory bowel disease / Catherine A. Abbott ... [et al.] Possible role of DP IV inhibitors in acne therapy / Anja Thielitz ... [et al.] Dipeptidyl peptidase-IV activity and/or structure homologs (DASH): contributing factors in the pathogenesis of rheumatic diseases? / Eva Balaziova ... [et al.] -- Topic V. Neuronal diseases. Dipeptidyl peptidase IV (DP IV, CD26) and aminopeptidase N (APN, CD13) as regulators of T cell function and targets of immunotherapy in CNS inflammation / Aliza Biton ... [et al.] CD26/DP IV in T cell activation and autoimmunity / Vera Preller ... [et al.] DPIV/CD26 and FAP in cancer: a tale of contradictions / Melanie L. Sulda, Catherine A. Abbott, and Martin Hildebrandt. Type-II transmembrane prolyl dipeptidases and matrix metalloproteinases in membrane vesicles of active endothelial cells / Monica Salamone ... [et al.] Extra-enzymatic roles of DPIV and FAP in cell adhesion and migration on collagen and fibronectin / Xin M. Wang ... [et al.] Role of neuropeptide Y and dipeptidyl peptidase IV in regulation of Ewing's sarcoma growth / Joanna Kitlinska ... [et al.] The role of CD26/DPP IV in preservation of early pulmonary graft function / Florian Johannes Jung ... [et al.].
    Also available: Print – 2006
  • 2002 Wiley
    Brakmann, Susanne; Johnsson, Kai.
    ch. 1. Introduction, p. 1-3 / Kai Johnsson and Susanne Brakmann, p. 1-3 -- ch. 2. Evolutionary Biotechnology - From Ideas and Concepts to Experiments and Computer Simulations / Peter Schuster, p. 5-28 -- ch. 3. Using Evolutionary Strategies to Investigate the Structure and Function of Chorismate Mutases / Donald Hilvert, Sean V. Taylor and Peter Kast, p. 29-62 -- ch. 4. Construction of Environmental Libraries for Functional Screening of Enzyme Activity / Rolf Daniel, p. 63-78 -- ch. 5. Investigation of Phage Display for the Directed Evolution of Enzymes / Patrice Soumillion and Jacques Fastrez, p. 79-110 -- ch. 6. Directed Evolution of Binding Proteins by Cell Surface Display: Analysis of the Screening Process / K. Dane Wittrup, p. 111-126 -- ch. 7. Yeast n-Hybrid Systems for Molecular Evolution / Brian T. Carter, Hening Lin and Virginia W. Cornish, p. 127-158 -- ch. 8. Advanced Screening Strategies for Biocatalyst Discovery / Andreas Schwienhorst, p. 159-175 -- ch. 9. Engineering Protein Evolution / Stefan Lutz and Stephen J. Benkovic, p. 177-213 -- ch. 10. Exploring the Diversity of Heme Enzymes through Directed Evolution / Patrick C. Cirino and Frances H. Arnold, p. 215-243 -- ch. 11. Directed Evolution as a Means to Create Enantioselective Enzymes for Use in Organic Chemistry / Manfred T. Reetz and Karl-Erich Jaeger, p. 245-279 -- ch. 12. Applied Molecular Evolution of Enzymes Involved in Synthesis and Repair of DNA / John F. Davidson, Jon Anderson, Haiwei Guo, Daniel Landis and Lawrence A. Loeb, p. 281-307 -- ch. 13. Evolutionary Generation Versus Rational Design of Restriction Endonucleases with Novel Specificity / Thomas Lanio, Albert Jeltsch and Alfred Pingoud, p. 309-327 -- ch. 14. Evolutionary Generation of Enzymes with Novel Substrate Specificities / Uwe T. Bornscheuer, p. 329-341.
  • 2006 CRCnetBASE
    McGrath, Barry M.; Walsh, Gary.
    Applied enzymology: an overview / Nancy Shanley and Gary Walsh -- Enzyme engineering / Barry M. McGrath -- Tissue plasminogen activator-based thrombolytic agents / Gary Walsh -- Activated protein C / Brian W. Grinnell, S. Betty Yan, and William L. Macias -- Deoxyribonuclease I / Niek N. Sanders, Stefaan C. de Smedt, and Joseph Demeester -- SS-glucocerebrosidase ceredase and cerezyme / Tim Edmunds -- A-galactosidase / Debra Barngrover -- Urate oxidase / Alain Bayol ... [et al.] -- L-asparaginase review of pharmacology, drug resistance, and clinical applications / Christine Mauz-Korholz, Volker Wahn, and Dieter Korholz -- Recombinant factor VIIa / Elisabeth Erhardtsen ... [et al.] -- Factor IX (protease zymogen) / Barry M. McGrath -- The development of aldurazyme (laronidase) / Emil Kakkis -- Additional therapeutic enzymes / Shane O'Connell.
  • 2013 Springer
    Spies, Maria.
    Also available: Print – 2013
  • 2005 CRCnetBASE
    Esteller, Manel.
  • 2009 Springer Protocols
    Tost, Jörg.
    DNA methylation : an introduction to the biology and the disease-associated changes of a promising biomarker / Jörg Tost -- Quantification of global DNA methylation by capillary electophoresis and mass spectrometry / Maria Berdasco, Mrio F. Fraga, and Manel Esteller -- Methyl group acceptance assay for the determination of global DNA methylation levels / Kenneth P. Nephew, Curt Balch, and David G. Skalnik -- Immunodetection array / Johannes Pröll ... [et al.] -- Methylated DNA immunoprecipitation (MeDIP) / Fabio Mohn ... [et al.] -- The MIRA method for DNA methylation analysis / Tibor A. Rauch and Gerd P. Pfeifer -- The HELP assay / Mayumi Oda and John M. Greally -- Differential methylation hybridization : profiling DNA methylation with a high-density CpG island microarray / Pearlly S. Yan ... [et al.] -- Analysis of DNA methylation by amplification of intermethylated sites (AIMS) / Mireia Jordà ... [et al.] -- Methylation-sensitive representational difference analysis (MS-RDA) / Toshikazu Ushijima and Satoshi Yamashita -- Restriction landmark genomic scanning : analysis of CpG islands in genomes by 2D gel elctrophoresis / Joseph F. Costello ... [et al.] -- GoldenGate assay for DNA methylation profiling / Marina Bibikova and Jian-Bing Fan -- 5'-azacytidine expression arrays / Paul Cairns -- DNA methylation analysis by bisulfite conversion, cloning, and sequencing of individual clones / Yingying Zhang ... [et al.] -- Identification and quantification of differentially methylated loci by the Pyrosequencing technology / Emelyne Dejeux ... [et al.] -- Mass spectrometric analysis of cytosine methylation by base-specific cleavage and primer extension methods / Dirk van den Boom and Mathias Ehrich -- Melting curve assays for DNA methylation analysis / Tomasz K. Wojdacz and Alexander Dobrovic -- Methylatioin SNaPshot : a method for the quantification of site-specific DNA methylation levels / Zachary Kaminsky and Arturas Petronis -- Bio-COBRA : absolute quantification of DNA methylation in electrofluidics chips / Romulo Martin Brena and Christoph Plass -- Restriction digestion and real-time PCR (qAMP) / Christopher C. Oakes ... [et al.] -- MethylQuant : a real-time PCR-based method to quantify DNA methylation at single specific cytosines / Claire Dugast-Darzacq and Thierry Grange -- Methylation-specific PCR / Julien D.F. Licchesi and James G. Herman -- MethyLight / Mihaela Campan ... [et al.] -- Quantification of methylated DNA by HeavyMethyl duplex PCR / Jürgen Distler -- Analysis of methylated circulating DNA in cancer patients' blood / Eiji Sunami ... [et al.] -- Prevention of PCR cross-contamination by UNG treatment of bisulfite-treated DNA / Reimo Tetzner -- Profiling DNA methylation from small amounts of genomic DNA starting material : efficient sodium bisulfite conversion and subsequent whole-genome amplification / Jonathan Mill and Arturas Petronis.
  • 2006 Springer
    Böhm, P.; Doerfler, Walter.
    Also available: Print – 2006
  • 2010 Springer
    Neve, Kim A.
    Also available: Print – 2010
  • 2008
    Nakamoto, Kazuo; Strahan, Gary D.; Tsuboi, Masamichi.
    This book provides vital information about drug-DNA interactions. Understand the types of structural and bonding information that can be obtained using specific physico-chemical methods and discover how to design new drugs that are more effective than current treatments and have fewer side effects. Find detailed information about X-ray crystallography, NMR spectroscopy, molecular modeling, and optical spectroscopy such as UV-Visible absorption, fluorescence, circular dichroism (CD), flow linear dichroism (FLD), infrared (IR) and Raman spectroscopy.
  • 2013 Springer
    Morré, D. James; Morré, Dorothy M.
    "ENOX Proteins" documents a unique family of cell surface proteins which are the exclusive discovery (subsequently confirmed by others) of the authors, their students and their colleagues. An ENOX-based mechanism is provided for how cells increase in size that is both unique and applicable not only to cancer and cancer therapy but also to production agriculture and biomass increase. The role of ENOX proteins in biological time-keeping is described along with evidence for oscillations in the ratios of electron spin pairs defining ortho and para water states that form highly coordinated populations of coherent water of interest to physicists as the underlying mechanism. The age-related NADH oxidases that appear around age 30 and increase steadily thereafter with potentially causal involvements in atherogenesis and skin aging have been identified as five members of the TM-9 protein superfamily of all with different chromosomal locations. The arNOX proteins initially are membrane anchored but functionally-active N-terminal fragments that are shed into body fluids. Except for critical functional motifs, all of the ENOX protein family members have distinct protein sequences and chromosomal origins. A fourth member of the ENOX protein family occurs in plants as the primary target for both natural and synthetic growth hormone (auxin)-stimulated rapid cell enlargement. Despite masquerading as intractable and difficult to clone and characterize, ENOX proteins offer remarkable opportunities for research, commercial development and outside confirmation of new paradigms to help explain complex biological processes.
  • 2002 CRCnetBASE
    Brajter-Toth, Anna; Chambers, James Q.
  • 2005 ScienceDirect
    Paleček, Emil; Scheller, F.; Wang, Joseph.
  • 2013 Springer Protocols
    Cheng, Yifan; Schmidt-Krey, Ingeborg.
    Introduction to electron crystallography / Werner Kuhlbrandt -- Practical aspects in expression and purification of membrane proteins for structural analysis / Kutti R. Vinothkumar, Patricia C. Edwards, and Joerg Standfuss -- Two-dimensional crystallization of membrane proteins by reconstitution through dialysis / Matthew C. Johnson [and others] -- Monolayer two-dimensional crystallization of membrane proteins / Luc Lebeau and Catherine Venien-Bryan -- Screening for two-dimensional crystals by transmission electron microscopy of negatively stained samples / Tina M. Dreaden [and others] -- Low dose techniques and cryo-electron microscopy / Yoshinori Fujiyoshi -- Grid preparation for cryo-electron microscopy / Nobuhiko Gyobu -- Recording high-resolution images of two-dimensional crystals of membrane proteins / Agustin Avila-Sakar [and others] -- Collection of high-resolution electron diffraction data / Tamir Gonen -- Image processing of 2D crystal images / Marcel Arheit [and others] -- Merging of image data in electron crystallography / Marcel Arheit [and others] -- Evaluation of electron crystallographic data from images of two-dimensional crystals / Vinzenz M. Unger -- Modeling, docking, and fitting of atomic structures to 3D maps from cryo-electron microscopy / Gregory S. Allen and David L. Stokes -- Phasing electron diffraction data by molecular replacement : strategy for structure determination and refinement / Goragot Wisedchaisri and Tamir Gonen -- High-throughput methods for electron crystallography / David L. Stokes [and others] -- Automated grid handling and image acquisition for two-dimensional crystal screening / Anchi Cheng -- Automation of data acquisition in electron crystallography / Anchi Cheng -- Automation of image processing in electron crystallography / Marcel Arheit [and others] -- Choice and maintenance of equipment for electron crystallography / Deryck J. Mills and Janet Vonck -- Future developments in instrumentation for electron crystallography / Kenneth H. Downing -- Tubular crystals and helical arrays : structural determination of HIV-1 capsid assemblies using iterative helical real-space reconstruction / Peijun Zhang, Xin Meng, and Gongpu Zhao -- Single particle electron microscopy / Wilson C.Y. Lau and John L. Rubinstein -- Electron tomography of paracrystalline 2D arrays / Hanspeter Winkler, Shenping Wu, and Kenneth A. Taylor -- High-resolution imaging of 2D outer membrane protein F crystals by atomic force microscopy / Dimitrios Fotiadis and Daniel J. Muller -- Determination of soluble and membrane protein structures by x-ray crystallography / Raquel L. Lieberman, Mary E. Peek, and J. Derrick Watkins -- Solution nuclear magnetic resonance spectroscopy / James J. Chou and Remy Sounier -- Structure-function insights of membrane and soluble proteins revealed by electron crystallography / Tina M. Dreaden [and others] -- Lipid monolayer and sparse matrix screening for growing two-dimensional crystals for electron crystallography : methods and examples / Mark Yeager, Kelly A. Dryden, and Barbie K. Ganser-Pornillos -- Processing of electron diffraction patterns with the XDP program / Kaoru Mitsuoka -- Future directions of electron crystallography / Yoshinori Fujiyoshi.
  • 2015 ScienceDirect
    v. 564 ScienceDirect
    Qin, Peter Z.; Warncke, Kurt.
  • 2007
    Brady, Catherine.
    Status: Not Checked OutLane Catalog Record
    A certain sense of self -- Shedding encumbrances -- One of Gall's gals -- Revelations -- Opportunism -- Gold rush -- Entering the fray -- An interlocking system -- Dr. Jekyll or Mr. Hyde? -- Members of a guild -- Citizen scientist -- Political fallout -- "You have to think it's fun" -- Notes -- Bibliography.
  • pt. A-<D>, 1987-<90>
    Dorian, A. F.
    Status: Not Checked OutLane Catalog Record
    pt. A. General medicine -- pt. B. Anatomy -- pt. C. Biology, genetics and biochemistry -- pt. D. Therapeutic substances.
  • 2012 ClinicalKey
    Pelley, John W.
    Bonus Student Consult access - included with the text - allows readers to conveniently access the book's content online. This concise and user-friendly reference provides crucial knowledge for the early years of medical training, as well as for exam preparation.
  • 2006 ScienceDirect
    Ricard, Jacques.
    Also available: Print – 2006
  • 2011
    Ufimtsev, Ivan S.; Markland, Thomas E.; Martinez, Todd J.; Pande, Vijay.
    The role of atomistic modeling of molecules and organic compounds in biology and pharmaceutical research is constantly increasing, providing insights on chemical and biological phenomena at the highest resolution. To achieve relevant results, however, computational biology has to deal with systems containing at least 1000 atoms. Such big molecules cause large computational demands and impose limitations on the level of theory used to describe molecular interactions. Classical molecular mechanics based on various empirical relationships has become a workhorse of computational biology, as a practical compromise between accuracy and computational cost. Several decades of classical force field development have seen many successes. Nevertheless, more accurate treatment of bio-molecules from first principles is highly desirable. Hartree-Fock (HF) and density functional theory (DFT) are two low-level ab initio methods that provide sufficient accuracy to interpret experimental data. They are therefore the methods of choice to study large biological systems. Recently DFT has been applied to calculate single point energy of a solvated Rubredoxin protein. The system contained 2825 atoms and required more than two hours on a supercomputer with 8196 parallel cores. This study clearly demonstrates the scale of problems one has to tackle in first principles calculations of biologically relevant systems. Dynamical simulations requiring thousands of single point energy and force evaluations therefore appear to be completely out of reach. This fact has essentially prohibited the use of first principles methods for many important biological systems. Fortunately, the computer industry is evolving quickly and novel computing architectures such as graphical processing units (GPUs) are emerging. The GPU is an indispensable part any modern desktop computer. It is special purpose hardware responsible for graphics processing. Most problems in computer graphics are embarrassingly parallel, meaning they can be split into a large number of smaller subproblems that can be solved in parallel. This fact has guided GPU development for more than a decade; and modern GPUs evolved into a massively parallel computing v architecture containing hundreds of basic computational units, which all together can perform trillions of arithmetic operations per second. The large computational performance and low price of consumer graphics cards makes it tempting to consider using them for computationally intensive general purpose computing. This fact was recognized long ago and several groups of enthusiasts attempted to use GPUs for non-graphics computing in the early 2000's. One of the few successes from these attempts is now known as Folding@Home. These early attempts were primarily stymied by three major problems: lack of adequate development frameworks, limited precision available on GPUs, and the difficulty of mapping existing algorithms onto the new architecture. The two former impediments have been recently alleviated by the introduction of efficient GPU programming toolkits such as CUDA and the latest generation of graphics cards supporting full double precision arithmetic operations in hardware. These advances led to an explosion of interest in general purpose GPU computing and led to the development of many GPU-based high performance applications in various fields such as classical molecular dynamics, magnetic resonance imaging, and computational fluid dynamics. Most of the projects, however, lie far outside of quantum chemistry which is likely caused by the complexity of quantum chemistry algorithms and the associated difficulty of mapping them onto the GPU architecture. Various specific features of the hardware require complete redesign of conventional HF and DFT algorithms in order to fully benefit from the large computational performance of GPUs. We have successfully solved this problem and implemented the new algorithms in TeraChem, a high performance general purpose quantum chemistry package designed for graphical processing units from the ground up. Using TeraChem, we performed the first ab initio molecular dynamics simulation of an entire Bovine pancreatic trypsin inhibitor (BPTI) protein for tens of picoseconds on a desktop workstation with eight GPUs operating in parallel. Coincidently, this was also the first protein ever simulated on a computer using the classical molecular mechanics approach. BPTI binds to trypsin with a binding free energy of approximately 20 kcal/mol, making BPTI one of the strongest non-covalent binders. It vi is even more remarkable that a single BPTI amino acid LYS15 contributes half of the binding free energy by forming a salt bridge with one of the trypsin's negatively charged residues inside the binding pocket. In fact, the LYS15's contribution to the overall binding energy is approximately twice as large as what would be expected based on experimental measurements of salt bridge interactions in other proteins. Our simulation of BPTI demonstrated that substantial charge transfer occurs at the proteinwater interface, where between 2.0 and 3.5 electrons are transferred from the interfacial water to the protein. This effect decreases the net protein charge from +6e as observed in gas-phase experiments to +4e or less. We demonstrate how this effect may explain the unusual binding affinity of the LYS15 amino acid.
  • Wiley
    A fully searchable, working editorial site of articles by scientists and scientific historians in the fields of biochemistry and physiology, cell biology, developmental biology, ecology, evolution, genetics, immunology, molecular biology, neuroscience, microbiology and virology, plant science, structural biology, and science and society.
  • 2013 Springer
    Kretsinger, Robert H.; Permi︠a︡kov, E. A.; Uversky, Vladimir N.
    In biochemistry, a metalloprotein is a generic term for a protein that contains a metal cofactor. The metal may be an isolated ion or may be coordinated with a nonprotein organic compound, such as the porphyrin found in hemoproteins. In some cases, the metal is co-coordinated with a side chain of the protein and an inorganic nonmetallic ion. This kind of protein-metal-nonmetal structure is seen in iron-sulfur clusters Metalloproteins deals with all aspects related to the intracellular and extracellular metal-binding proteins, including their structures, properties and functions. The biological roles of metal cations and metal-binding proteins are endless. They are involved in all crucial cellular activities. Many pathological conditions are related to the problematic metal metabolism. Research in metalloprotein-related topics is therefore rapidly growing, and different aspects of metal-binding proteins progressively enter curricula at Universities and even at the High School level on occasion. However, no key resource providing basic, but comprehensible knowledge on this rapidly expanding field exists. The Encyclopedia of Metalloproteins aims to bridge this gap, and will attempt to cover various aspects of metalloprotein/metalloproteomics and will deal with the different issues related to the intracellular and extracellular metal-binding proteins, including their structures, properties and functions. The goal is to cover exhaustively all catalytically and biologically crucial metal ions and to find at least one interacting protein for other metal ions. The Encyclopedia of Metalloproteins will provide a key resource for advanced undergraduate and graduate students, researchers, instructors, and professors interested in protein science, biochemistry, cell biology, and genetics.
  • 2012 Springer
    Kuro-o, Makoto.
    The Structural Biology of the FGF19 Subfamily / Andrew Beenken and Moosa Mohammadi -- Klotho and βKlotho / Makoto Kuro-o -- FGF23 and Syndromes of Abnormal Renal Phosphate Handling / Clemens Bergwitz and Harald Jüppner -- Evidence for FGF23 Involvement in a Bone-Kidney Axis Regulating Bone Mineralization and Systemic Phosphate and Vitamin D Homeostasis / Aline Martin and L. Darryl Quarles -- FGF23, Klotho and Vitamin D Interactions: What Have We Learned from In Vivo Mouse Genetics Studies? / M. Shawkat Razzaque -- FGF23 and the Parathyroid / Justin Silver and Tally Naveh-Many -- Regulation of Ion Channels by Secreted Klotho / Chou-Long Huang -- FGF23 in Chronic Kidney Disease / Patricia Wahl and Myles Wolf -- Secreted Klotho and Chronic Kidney Disease / Ming Chang Hu, Makoto Kuro-o and Orson W. Moe -- FGF23 as a Novel Therapeutic Target / Takashi Shimada and Seiji Fukumoto -- Physiology of FGF15/19 / Stacey A. Jones -- FGF19 and Cancer / Benjamin C. Lin and Luc R. Desnoyers -- Understanding the Structure-Function Relationship between FGF19 and Its Mitogenic and Metabolic Activities / Xinle Wu and Yang Li -- FGF21 as a Therapeutic Reagent / Yang Zhao, James D. Dunbar and Alexei Kharitonenkov.
    Also available: Print – 2012
  • 2010 Springer
    Quinn, Peter J.; Wang, Xiaoyuan.
    Endotoxins: Lipopolysaccharides of Gram-negative bacteria / X. Wang and P.J. Quinn -- Purification and characterization of lipopolysaccharides / X. Wang ... [et al.] -- Endotoxins: Relationship between structure, function, and activity / K. Brandenburg, A.B. Schromm and T. Gutsmann -- The diversity of the core oligosaccharide in lipopolysaccharides / A. Silipo and A. Molinaro -- Salmonella-regulated lipopolysaccharide modifications / S.M. Richards, K.L. Strandberg and J.S. Gunn -- The variation of O antigens in Gram-negative bacteria / L. Wang, Q. Wang and P.R. Reeves -- Regulators of TLR4 signaling by endotoxins / A.F. McGettrick and L.A.J. O'Neill -- Membrane partitioning: Is location everything when it comes to endotoxin recognition? / M. Triantafilou and K. Triantafilou -- Endotoxin detection - from limulus amebocyte lysate to recombinant Factor C / J.L. Ding and B. Ho -- The role of endotoxin in infection: Helicobacter pylori and Campylobacter jejuni / A.P. Moran -- The role of pseudomonas lipopolysaccharide in cystic fibrosis airway infection / S.M. Moskowitz and R.K. Ernst -- Development of small-molecule endotoxin sequestering agents / S.A. David and D. Sil -- Development of an anti-endotoxin vaccine for sepsis / A.S. Cross -- Synthetic and natural TLR4 agonists as safe and effective vaccine adjuvants / C.B. Fox ... [et al.] -- Targeting endotoxin in the treatment of sepsis / J.-S. Rachoin, C.A. Schorr and R.P. Dellinger -- Lipopolysaccharides in rhizobium-legume symbioses / R.W. Carlson, L.S. Forsberg and E.L. Kannenberg -- Lipopolysaccharides and plant innate immunity / G. Erbs ... [et al.].
  • 2010 Springer Protocols
    Mackay, Joel P.; Segal, David J.
    The generation of zinc finger proteins by modular assembly / Mital S. Bhakta and David J. Segal -- Engineering single Cys2His2 zinc finger domains using a bacterial cell-based two-hybrid selection system / Stacey Thibodeau-Beganny, Morgan L. Maeder, and J. Keith Joung -- Bipartite selection of zinc fingers by phage display for any 9-bp DNA target site / Jia-Ching Shieh -- Structure-based DNA-binding prediction and design / Andreu Alibés, Luis Serrano, and Alejandro D. Nadra -- Generation of cell-permeable artificial zinc finger protein variants / Takashi Sera -- Inhibition of viral transcription using designed zinc finger proteins / Kimberley A. Hoeksema and D. Lorne J. Tyrrell -- Modulation of gene expression using zinc finger-based artificial transcription factors / Sabine Stolzenburg ... [et al.] -- Construction of combinatorial libraries that encode zinc finger-based transcription factors / Seokjoong Kim, Eun Ji Kim, and Jin-Soo Kim -- Silencing of gene expression by targeted DNA methylation : concepts and approaches / Renata Z. Jurkowska and Albert Jeltsch -- Remodeling genomes with artificial transcription factors (ATFs) / Adriana S. Beltran and Pilar Blancafort -- Transgenic mice expressing an artificial zinc finger regulator targeting an endogenous gene / Claudio Passananti ... [et al.] -- Artificial zinc finger nucleases for DNA cloning / Vardit Zeevi, Andriy Tovkach, and Tzvi Tzfira -- In vitro assessment of zinc finger nuclease activity / Toni Cathomen and Cem Șöllü -- Quantification of zinc finger nuclease-associated toxicity / Tatjana I. Cornu and Toni Cathomen -- A rapid and general assay for monitoring endogenous gene modification / Dmitry Y. Guschin ... [et al.] -- Engineered zinc finger proteins for manipulation of the human mitochondrial genome / Michal Minczuk -- High-efficiency gene targeting in Drosophila with zinc finger nucleases / Dana Carroll, Kelly J. Beumer, and Jonathan K. Trautman -- Using zinc finger nucleases for efficient and heritable gene disruption in zebrafish / Jasmine M. McCammon and Sharon L. Amacher -- A transient assay for monitoring zinc finger nuclease activity at endogenous plant gene targets / Justin P. Hoshaw ... [et al.] -- Validation and expression of zinc finger nucleases in plant cells / Andriy Tovkach, Vardit Zeevi, and Tzvi Tzfira -- Non-FokI-based zinc finger nucleases / Miki Imanishi, Shigeru Negi, and Yukio Sugiura -- Designing and testing chimeric zinc finger transposases / Matthew H. Wilson and Alfred L. George -- Seeing genetic and epigenetic information without DNA denaturation using sequence-enabled reassembly (SEER) / Jason R. Porter ... [et al.] -- Zinc finger-mediated live cell imaging in Arabidopsis roots / Beatrice I. Lindhout, Tobias Meckel, and Bert J. van der Zaal -- Biophysical analysis of the interaction of toxic metal ions and oxidants with the zinc finger domain of XPA / Andrea Hartwig, Tanja Schwerdtle, and Wojciech Bal -- Preparation and zinc-binding properties of multi-fingered zinc-sensing domains / John H. Laity and Linda S. Feng -- Using ChIP-seq technology to identify targets of zinc finger transcription factors / Henriette O'Geen, Seth Frietze, and Peggy J. Farnham -- Crystallization of zinc finger proteins bound to DNA / Nancy C. Horton and Chad K. Park -- Beyond DNA : zinc finger domains as RNA-binding modules / Josep Font and Joel P. Mackay.
  • 2013 Springer
    Divakar, Soundar.
    Transformations using enzymes have been extensively investigated in the last two decades and the results promise great potential for this growing field, especially in the area of synthetic organic chemistry mainly due to of its many advantages. Accordingly, this book has attempted to bring out the advantages of using enzymes involving complex underivatized and unprotected substrates in non-polar media under homogenous and heterogeneous reaction conditions. Merits and demerits of using enzymes in terms of yields and selectivity/specificity are presented without any prejudice. Almost all the reactions dealt with are from the author's laboratory comprising diverse substrates, and the catalysis involves two important hydrolyzing enzymes, extensively examined for the reverse reactions. Thus, esterification involving lipses and glycosylation involving glycosidases were investigated with respect to various strategies like optimization of reaction conditions, response surface methodology and kinetics, carrying out reactions under solvent, non-solvent and super critical carbon dioxide conditions. In short, the work presented is to ensure the comprehension of the problems faced by the researchers in this area so as to work out further efficient strategies for carrying out enzymatic transformations in the laboratory successfully with better yields and specificity.
  • 2013 Springer Protocols
    Samuelson, James C.
    1. Tripartite fusion system for the selection of protein variants with increased stability in vivo / Linda Foit and James C.A. Bardwell -- 2. Determining enzyme kinetics via isothermal titration calorimetry / Neil A. Demarse, Marie C. Killian, Lee D. Hansen and Colette F. Quinn -- 3. GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria / Olga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo, George Georgiou and Everett Stone -- 4. Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties / Sarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg and Jordan Jarjour -- 5. TAL effector nuclease (TALEN) engineering / Ting Li and Bing Yang -- 6. In vitro evolution of enzymes / Misha V. Golynskiy, John C. Haugner III, Aleardo Morelli, Dana Morrone and Burckhard Seeling -- 7. Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo / Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington -- 8. Reconstructing evolutionary adaptive paths for protein engineering / Megan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, and Eric A. Gaucher -- 9. Oligonucleotide recombination enabled site-specific mutagenesis in bacteria / Bryan M. Swingle -- 10. FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants / Eric R. Geertsma -- 11. Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes / Hidehiko Hirakawa and Teruyuki Nagamune -- 12. Gene synthesis by assembly of deoxyuridine-containing oligonucleotides / Romualdas Vaisvila and Jurate Bitinaite -- 13. Protein engineering : single or multiple site-directed mutagenesis / Pei-Chung Hsieh and Romualdas Vaisvila -- 14. Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination / Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish -- 15. Promiscuity-based enzyme selection for rational directed evolution experiments / Sandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. Dandekar, Swapan K. Bhattacharjee and Basuthkar J. Rao -- 16. Rational protein sequence diversification by multi-codon scanning mutagenesis / Jia Liu and T. Ashton Cropp -- 17. Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter / Jian-Wei Liu and David L. Ollis -- 18. In vitro directed evolution of enzymes expressed by E. coli in microtiter plates / Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis.
  • 2010 ScienceDirect
    Purich, Daniel L.
    Chapter 1. An Introduction to Enzyme Science -- Chapter 2. Active Sites and their Chemical Properties -- Chapter 3. Fundamentals of Chemical Kinetics -- Chapter 4. Practical Aspects of Measuring Initial Rates and Reaction Parameters -- Chapter 5. Initial-Rate Kinetics of One-Substrate Enzyme-Catalyzed Reactions -- Chapter 6. Initial-Rate Kinetics of Multi-Substrate Enzyme-Catalyzed Reactions -- Chapter 7. Factors Influencing Enzyme Activity -- Chapter 8. Kinetic Behavior of Enzyme Inhibitors -- Chapter 9. Isotopic Probes of Biological Catalysis -- Chapter 10. Probing Fast Enzyme Processes -- Chapter 11. Regulatory Behavior of Enzymes -- Chapter 12. Single-Molecule Enzyme Kinetics -- Chapter 13. Mechanoenzymes: Catalysis, Force Generation and Kinetics.
  • 2002 Wiley
    Bisswanger, Hans; Bubenheim, Leonie.
    Introduction, p. 1-3 -- ch. 1. Multiple Equilibria, p. 5-50 -- ch. 2. Enzyme Kinetics, p. 51-160 -- ch. 3. Methods, p. 161-246.
  • 2011 Wiley
    Alberty, Robert A.
    Also available: Print – v. 53, 2011.
  • 2002 ScienceDirect
    Purich, Daniel L.
    Also available: Print – 2002
  • 2011 Springer Protocols
    Minteer, Shelley D.
    Introduction to the field of enzyme immobilization and stabilization -- Stabilization of enzymes through encapsulation in liposomes -- Micellar enzymology for thermal, pH, and solvent stability -- Lipase activation and stabilization in room-temperature ionic liquids -- Nanoporous silica glass for the immobilization of interactive enzyme systems -- Enzyme stabilization and immobilization by sol-gel entrapment -- Nanoporous gold for enzyme immobilization -- Enzyme stabilization via bio-templated silicification reactions -- The immobilization of enzyme on Eupergit) supports by covalent attachment -- Micellar polymer encapsulation of enzymes -- Enzyme stabilization via cross-linked enzyme aggregates -- Enzyme immobilization in polyelectrolyte microcapsules -- Macroporous poly(GMA-co-EGDMA) for enzyme stabilization -- Enzyme-nanoparticle conjugates for biomedical applications -- Enzyme nanoparticle fabrication: Magnetic nanoparticle synthesis and enzyme immobilization -- Cytochrome C stabilization and immobilization in aerogels -- Kinetic measurements for enzyme immobilization.
  • 2002 CRCnetBASE
    Burns, Richard G.; Dick, Richard P.
    1. Enzyme Activities and Microbiological and Biochemical Processes in Soil / Paolo Nannipieri, Ellen Kandeler, and Pacifico Ruggiero -- 2. Ecology of Microbial Enzymes in Lake Ecosystems / Ryszard Jan Chróst and Waldemar Siuda -- 3. Ecological Significance of Bacterial Enzymes in the Marine Environment / Hans-Georg Hoppe, Carol Arosti, and Gerhard F. Herndl -- 4. Enzymes and Microorganisms in the Rhizosphere / David C. Naseby and James M. Lynch -- 5. Enzymes in the Arbuscular Mycorrhizal Symbiosis / José Manuel García-Garrido, Juan Antonio Ocampo, and Inmaculada García-Romera -- 6. Microbes and Enzymes Associated with Plant Surfaces / Alan P. Thompson and Mark J. Bailey -- 7. Microbial Enzymes in the Biocontrol of Plant Pathogens and Pests / Leonid Cherin and Ilan Chet -- 8. Microbiology and Enzymology of Carbon and Nitrogen Cycling / Robert L. Tate III -- 9. Enzyme and Microbial Dynamics of Litter Decomposition / Robert L. Sinsabaugh, Margaret M. Carreiro, and Sergio Alvarez -- 10. Fungal Communities, Succession, Enzymes, and Decomposition / Annelise H. Kjøller and Sten Struwe -- 11. Enzyme Adsorption on Soil Mineral Surfaces and Consequences for the Catalytic Activity / Hervé Quiquampoix, Sylvie Servagent-Noinville, and Marie-Hélène Baron -- 12. Microbes and Enzymes in Biofilms / Jana Jass, Sara K. Roberts, and Hilary M. Lappin-Scott -- 13. Search for and Discovery of Microbial Enzymes from Thermally Extreme Environments in the Ocean / Jody W. Deming and John A. Baross -- 14. Molecular Methods for Assessing and Manipulating the Diversity of Microbial Populations and Processes / Søren J. Sørensen, Julia R. de Lipthay, Anne Kirstine Müller, Tamar Barkay, Lars H. Hansen, and Lasse Dam Rasmussen -- 15. Bioindicators and Sensors of Soil Health and the Application of Geostatistics / Ken Killham and William J. Staddon -- 16. Hydrolytic Enzyme Activities to Assess Soil Degradation and Recovery / Tom W. Speir and Des J. Ross -- 17. Enzymatic Responses to Pollution in Sediments and Aquatic Systems / Sabine Kuhbier, Hans-Joachim Lorch, and Johannes C.G. Ottow -- 18. Microbial Dehalogenation Reactions in Microorganisms / Lee A. Beaudette, William J. Staddon, Michael B. Cassidy, Marc Habash, Hung Lee, and Jack T. Trevors -- 19. Isolated Enzymes for the Transformation and Det6xification of Organic Pollutants / Liliana Gianfreda and Jean-Marc Bollag -- 20. Enzyme-Mediated Transformations of Heavy Metals/Metalloids: Applications in Bioremediation / Robert S. Dungan and William T. Frankenberger, Jr. -- 21. Enzymes in Soil: Research and Developments in Measuring Activities / M. Ali Tabatabai and Warren A. Dick.
  • 2007 Springer
    Berliner, Lawrence J.; Hemminga, Marcus A.
  • 2012 Springer
    MacNeill, Stuart.
    Composition and Dynamics of the Eukaryotic Replisome: A Brief Overview / Stuart MacNeill -- Evolutionary Diversification of Eukaryotic DNA Replication Machinery / Stephen J. Aves, Yuan Liu and Thomas A. Richards -- The Origin Recognition Complex: A Biochemical and Structural View / Huilin Li and Bruce Stillman -- Archaeal Orc1/Cdc6 Proteins / Stephen D. Bell -- Cdt1 and Geminin in DNA Replication Initiation / Christophe Caillat and Anastassis Perrakis -- MCM Structure and Mechanics: What We Have Learned from Archaeal MCM / Ian M. Slaymaker and Xiaojiang S. Chen -- The Eukaryotic Mcm2-7 Replicative Helicase / Sriram Vijayraghavan and Anthony Schwacha -- The GINS Complex: Structure and Function / Katsuhiko Kamada -- The Pol [alpha]-Primase Complex / Luca Pellegrini -- The Structure and Function of Replication Protein A in DNA Replication / Aishwarya Prakash and Gloria E. O. Borgstahl -- Structural Biology of Replication Initiation Factor Mcm10 / Wenyue Du, Melissa E. Stauffer and Brandt F. Eichman -- Structure and Function of Eukaryotic DNA Polymerase [delta] / Tahir H. Tahirov -- DNA Polymerase [epsilon] / Matthew Hogg and Erik Johansson -- The RFC Clamp Loader: Structure and Function / Nina Y. Yao and Mike O'Donnell -- PCNA Structure and Function: Insights from Structures of PCNA Complexes and Post-translationally Modified PCNA / Lynne M. Dieckman, Bret D. Freudenthal and M. Todd Washington -- The Wonders of Flap Endonucleases: Structure, Function, Mechanism and Regulation / L. David Finger, John M. Atack, Susan Tsutakawa, Scott Classen and John Tainer, et al. -- DNA Ligase I, the Replicative DNA Ligase / Timothy R. L. Howes and Alan E. Tomkinson.
  • NCBI Bookshelf
  • 2012 Springer
    Navard, Patrick.
    This book provides an essential overview of the science of polysaccharides. It both approaches polysaccharides as a polymer class and provides detailed descriptions of most major polysaccharides (cellulose, mannan, xylan, chitin-chitosan, cyclodextrines). Owing to the multidisciplinary character of the European Polysaccharide Network of Excellence (EPNOE), the book describes all main aspects of polysaccharide science and technology (biology, enzymology, physics, chemistry, materials science and processing). Notations and concepts follow a uniform format throughout the whole work in order to create a valuable reference book on the field of polysaccharide science. Owing to the major importance of industry in the EPNOE, concrete applications are also described in detail.
  • 2013 Wiley
    Copeland, Robert Allen.
    "There has been explosive growth in the hunt for new pharmaceutically agents globally. Traditionally, this has been the purview of the pharmaceutical industry, but today, this effort crosses academic, government, and industry laboratories across the world. Enzymes remain the most valued and common of drug targets; hence, a detailed understanding of their interactions with inhibitors is critical to successful drug discovery. This book provides a practical, readable, and comprehensive treatment of these topics that allows scientists to master the art of applied enzymology for drug discovery. The book addresses the opportunities for inhibitor interactions with enzyme targets arising from consideration of the catalytic reaction mechanism; discusses how inhibitors are properly evaluated for potency, selectivity, and mode of action, covers the potential advantages and liabilities of specific inhibition modalities with respect to efficacy in vivo, and provides valuable biochemical insights to help medicinal chemists and pharmacologists most effectively pursue lead optimization. It includes two new chapters, one on the pioneering idea of drug-target residence time fostered by Dr. Copeland, and the second on quantitative biochemistry. Five new appendices are added"--Provided by publisher.
  • 2006 CRCnetBASE
    Lundblad, Roger L.
  • 2008 Springer
    Taniguchi, Naoyuki.
  • 2011 Springer
    Mecham, Robert P.; Parks, William C.
    Matrix metalloproteinases and their inhibitors in turnover and degradation of extracellular matrix / Sean E. Gill and William C. Parks -- Role of cysteine cathepsins in extracellular proteolysis / Dieter Brömme and Susan Wilson -- Cooperation between proteolysis and endocytosis in collagen turnover / Thomas H. Bugge and Niels Behrendt -- Pericellular proteolysis / Judith S. Bond, Timothy R. Keiffer, and Qi Sun -- Triple helicase activity and the structural basis of collagenolysis / Hideaki Nagase and Robert Visse -- Structural basis of extracellular matrix interactions with matrix metalloproteinases / Steven R. Van Doren -- The role of matrix metalloproteinases in cellular invasion and metastasis / Elena I. Deryugina and James P. Quigley -- Degradation of bone and the role of osteoclasts, bone lining cells and osteocytes / Vincent Everts and Paul Saftig -- Consequences of elastolysis / A. McGarry Houghton, Majd Mouded, and Steven D. Shapiro.
    Also available: Print – 2011
  • 2009 Springer Protocols
    Artym, Vira V.; Even-Ram, Sharona; Streuli, Charles H.
    Retroviral delivery of ECM genes / Vitali alexeev and Olga Igoucheva -- Tissue-specific KO of ECM proteins / Mara Brancaccio, Emila Turco, and Emilio Hirsch -- Recombinant collagen trimers from insect cells and yeast / Johanna Myllyharju -- Eukaryotic expression and purification of recombinant extracellular matrix proteins carrying the Strep II tag / Neil Smyth ... [et al.] -- Preparation of recombinant fibronectin fragments for functional and structural studies / David Staunton ... [et al.] -- Quantitative determination of collagen cross-links / Nicholas C. Avery, Trevor J. Sims, and Allen J. Bailey -- ECM macromolecules : height-mapping and nano-mechanics using atomic force microscopy / Nigel W. Hodson, Cay M. Kielty, and Michael J. Sherratt -- Atomic force microscopy measurements of intermolecular binding forces / Gradimir N. Misevic, Yannis Karamanos, and Nikola J. Misevic -- Mass-mapping of ECM macromolecules by scanning transmission electron microscopy / Michael J. Sherratt ... [et al.] -- Chemical microscopy of biological samples by dynamic mode secondary ion mass spectrometry / Gradimir N. Misevic ... [et al.] -- ECM macromolecules : rotary shadowing and transmission electron microscopy / Michael J. Sherratt ... [et al.] -- Using self-assembled monolayers to pattern ECM proteins and cells on substrates / Emanuele Ostuni ... [et al.] -- Solid phase assays for studying ECM protein-protein interactions / A. Paul Mould -- Cell adhesion assays / Martin J. Humphries -- ECM degradation assays for analyzing local cell invasion / Vira V. Artym, Kenneth M. Yamada, and Susette C. Mueller -- Fluorescence-based assays for in vitro analysis of cell adhesion and migration / Paola Spessotto ... [et al.] -- Fibrin gel model for assessment of cellular contractility / Sharona Even-Ram -- Fluorescent labeling techniques for investigation of fibronectin fibrillogenesis (labeling fibronectin fibrillogenesis) / Roumen Pankov and Albena Momchilova -- Stromagenesis during tumorigenesis : characterization of tumor-associated fibroblasts and stroma-derived 3D matrices / Remedios Castelló-Cros and Edna Cukierman -- Tissue recombinants to study extracellular matrix targeting to basement membranes / Patricia Simon-Assmann ... [et al.] -- ECM and FGF-dependent assay of embryonic SMG epithelial morphogenesis : investigating growth factor/matrix regulation of gene expression during submandibular gland development / Ivan T. Rebustini and Matthew P. Hoffman -- Analyzing how cell adhesion controls mammary gland function by transplantation of embryonic mammary tissue from knockout mice / Teresa C.M. Klinowska and Charles H. Streuli -- Characterizing ECM production by cells encapsulated in hydrogels / Iossif A. Strehin and Jennifer H. Elisseeff -- Tissue engineering and cell-populated collagen matrices / Paul D. Kemp.
  • 2010 Springer
    Kikuchi, Yo; Rykova, Elena Y.
    Extracellular DNA as Matrix Component in Microbial Biofilms / Wen-Chi Chiang and Tim Tolker-Nielsen -- Gene Transfer Agents and Defective Bacteriophages as Sources of Extracellular Prokaryotic DNA / Andrew S. Lang and J. Thomas Beatty -- Roles of Extracellular DNA in Bacterial Ecosystem / Katsuji Tani and Masao Nasu -- Stable Extracellular DNA: A Novel Substrate for Genetic Engineering that Mimics Horizontal Gene Transfer in Nature / Shinya Kaneko and Mitsuhiro Itaya -- Extracellular Nucleic Acids of the Marine Phototrophic Bacterium Rhodovulum sulfidophilum and Related Bacteria: Physiology and Biotechnology / Yo Kikuchi -- Systemic RNAi in C. elegans from the Viewpoint of RNA as Extracellular Signals / Lisa Timmons -- Circulating Nucleic Acids in Health and Disease / E. Y. Rykova, P. P. Laktionov and V. V. Vlassov -- Circulating MicroRNAs in Cancer / Patrick S. Mitchell and Muneesh Tewari -- Biology and Diagnostic Applications of Cell-Free Fetal Nucleic Acids in Maternal Plasma / Jason C. H. Tsang and Y. M. Dennis Lo -- The Biology of Circulating Nucleic Acids in Plasma and Serum (CNAPS) / P. B. Gahan and M. Stroun -- Modulation and Regulation of Gene Expression by CpG Oligonucleotides / Dennis Klinman and Sven Klaschik -- Immune Recognition of Nucleic Acids and Their Metabolites / Shohei Koyama, Shizuo Akira and Ken J. Ishii.
  • 2012 Springer
    Kotb, Essam.
  • 2014 Springer Protocols
    Schleicher, Erik; Weber, Stefan.
    Structure and General Properties of Flavins -- Recent advances in Riboflavin Biosynthesis -- Natural Riboflavin Analogs -- A Roadmap to the Isotopolog Space of Flavocoenzymes -- Electron Transferases -- Aldonolactone Oxidoreductases -- Flavins and Flavoproteins: Applications in Medicine -- Practical Aspects on the Use of Kinetic Isotope Effects as Probes of Flavoprotein Enzyme Mechanisms -- On the in vivo Redox State of Flavin-Containing Photosensory Receptor Proteins -- Computational Spectroscopy, Dynamics and Photochemistry of Photosensory Flavoproteins -- NMR Spectroscopy on Flavins and Flavoproteins -- Solid-State NMR of Flavins and Flavoproteins.-EPR on Flavoproteins -- FTIR Spectroscopy of Flavin-Binding Photoreceptors -- Resonance Raman Spectroscopy -- Photoactivation Mechanisms of Flavin-binding Photoreceptors Revealed through Ultrafast Spectroscopy and Global Analysis Methods -- Excited State Chemistry of Flavoproteins. .
  • 2012 CRCnetBASE
    Crozier, Alan; Spencer, Jeremy P .E.
    Bioavailability of flavanones / Mireia Urpi-Sarda ... [et al.] -- Bioavailability of dietary monomeric and polymeric flavan-3-ols / Alan Crozier, Michael N. Clifford, and Daniele Del Rio -- Anthocyanins : understanding their absorption and metabolism / Ronald L. Prior -- Bioavailability of flavonols and flavones / Mariusz Konrad Piskula, Kaeko Murota, and Junji Terao -- Bioavailability of isoflavones in humans / Aedín Cassidy, José Peñalvo, and Peter Hollman -- Dietary hydroxycinnamates and their bioavailability / Angelique Stalmach, Gary Williamson, and Mike Clifford -- Bioavailability of dihydrochalcones / Elke Richling -- Occurrence, bioavailability, and metabolism of resveratrol / Paola Vitaglione, Stefano Sforza, and Daniele Del Rio -- Bioavailability and metabolism of ellagic acid and ellagitannins / Mar Larrosa ... [et al.] -- Colon-derived microbial metabolites of dietary phenolic compounds / Anna-Marja Aura -- Synthesis of dietary phenolic metabolites and isotopically labeled dietary phenolics / Denis Barron ... [et al.] -- Interactions of flavan-3-ols within cellular signaling pathways / Cesar G. Fraga and Patricia I. Oteiza -- Flavonoids and vascular function / Ana Rodriguez-Mateos and Jeremy P.E. Spencer -- Effects of flavonoids on the vascular endothelium : what is known and what is next? / Antje R. Weseler and Aalt Bast -- Green tea flavan-3-ols and their role in protecting against Alzheimer's and Parkinson's disease pathophysiology / Orly Weinreb ... [et al.] -- Flavonoids and neuroinflammation / David Vauzour and Katerina Vafeiadou -- Effects of flavonoids on cognitive performance / Shibu M. Poulose and Barbara Shukitt-Hale -- Flavonoids and oral cancer / Thomas Walle -- Flavonoids and cancer-effects on dna damage / Piyawan Sitthiphong ... [et al.].
  • 2010 ClinicalKey
    Halperin, M. L.; Goldstein, Marc B.; Kamel, Kamel S.
    Principles of acid-base physiology -- Tools to use to diagnose acid-base disorders -- Metabolic acidosis: clinical approach -- Metabolic acidosis due to a deficit of NaHCO₃ -- Ketoacidosis -- Metabolic acidosis: acid gain types -- Metabolic alkalosis -- Respiratory acid-base disturbances -- Sodium and waterphysiology -- Hyponatremia -- Hypernatremia -- Polyuria -- Potassium physiology -- Hypokalemia -- Hyperkalemia -- Hyperglycemia.
    Also available: Print – 2010
  • 2007 Springer
    Ishihara, Hironori; Giesecke, A. H.
  • 2008 ScienceDirect
    Brand, Ludwig; Johnson, Michael L.
    A method in enzymology for measuring hydrolytic activities in live cell environments / Beverly Z. Packard and Akira Komoriya -- Heterogeneity of fluorescence determined by the method of area-normalized time-resolved emission spectroscopy / N. Periasamy -- Multiparametric probing of microenvironment with solvatochromic fluorescent dyes / Andrey S. Klymchenko and Alexander P. Demchenko -- Site-selective red-edge effects / Alexander P. Demchenko -- Fluorescence approaches to quantifying biomolecular interactions / Catherine A. Royer and Suzanne F. Scarlata -- Forster resonance energy transfer measurements of transmembrane helix dimerization energetics / Mikhail Merzlyakov and Kalina Hristova -- Application of single-molecule spectroscopy in studying enzyme kinetics and mechanism / Jue Shi ... [et al.] -- Ultrafast fluorescence spectroscopy via upconversion : applications to biophysics / Jianhua Xu and Jay R. Knutson -- Use of fluorescence resonance energy transfer (FRET) in studying protein-induced DNA bending / Anatoly I. Dragan and Peter L. Privalov --Fluorescent pteridine probes for nucleic acid analysis / Mary E. Hawkins -- Single-molecule fluorescence methods for the analysis of RNA folding and ribonucleoprotein assembly / Goran Pljevaljčić and David P. Millar -- Using fluorophore-labeled oligonucleotides to measure affinities of protein-DNA interactions / Brian J. Anderson ... [et al.] -- Identifying small pulsatile signals within noisy data : a fluorescence application / Michael L. Johnson ... [et al.] -- Determination of zinc using carbonic anhydrase-based fluorescence biosensors / Rebecca Bozym ... [et al.] -- Instrumentation for fluorescence-based fiber optic biosensors / Richard B. Thompson ... [et al.]
    Also available: Print – 2008
  • 2014 Springer
    Fili, Natalia; Toseland, Christopher P.
    This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.
  • 2014 Springer Protocols
    Newman, Robert H.; Ni, Qiang; Zhang, Jin.
    Design and application of genetically encodable biosensors based on fluorescent proteins / Robert H. Newman and Jin Zhang -- Introduction to fluorescence imaging techniques geared towards biosensor applications / J. Goedhart, Mark A. Hink, and Kees Jalink -- Quantitative measurement of Ca²⁺ and Zn²⁺ in mammalian cells using genetically encoded fluorescent biosensors / J. Genevieve Park and Amy E. Palmer -- Detecting cAMP with an epac-based FRET sensor in single living cells / J. Klarenbeek and Kees Jalink -- Analysis of compartmentalized cAMP : a method to compare signals from differently targeted FRET reporters / Alessandra Stangherlin ... [et al.] -- Genetically encoded fluorescent biosensors for live cell imaging of lipid dynamics / Moritoshi Sato -- Live-cell imaging of cytosolic NADH-NAD⁺ redox state using a genetically encoded fluorescent biosensor / Yin Pun Hung and Gary Yellen -- Measuring membrane voltage with microbial rhodopsins / Adam E. Cohen and Daniel R. Hochbaum -- Imaging the activity of Ras superfamily GTPase proteins in small subcellular compartments in neurons / Ana F. Oliveira and Ryohei Yasuda -- Imaging kinase activity at protein scaffolds / Maya T. Kunkel and Alexandra C. Newton -- Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells / Sohum Mehta and Jin Zhang -- Genetically encoded FRET indicators for live-cell imaging of histone acetylation / Kazuki Sasaki and Minoru Yoshida -- Genetically encoded fluorescent biosensors for live-cell imaging of MT1-MMP protease activity / Mingxing Ouyang, Shaoying Lu, and Yingxiao Wang -- Biosensor imaging in brain slice preparations / Marina Polito, Pierre Vincent, and Elvire Guiot -- Optical calcium imaging using DNA-encoded fluorescence sensors in transgenic fruit flies, Drosophila melanogaster / Shubham Dipt, Thomas Riemensperger, and André Fiala -- Multiparameter live cell imaging approach to monitor cyclic AMP and protein kinase a dynamics in parallel / Nwe-Nwe Aye-Han and Jin Zhang -- FRET and BRET-based biosensors in live cell compound screens / Katie Herbst Robinson, Jessica R. Yang, and Jin Zhang -- Integrating fluorescent biosensor data using computational models / Eric C. Greenwald, Renata K. Polanowska-Grabowska, and Jeffrey J. Saucerman.
  • 2011 Springer
    O'Connor, Vincent; Wyttenbach, Andreas.
    Folding for the Synapse addresses the current view on how protein folding and misfolding, controlled by molecular chaperones, contribute to synapse function and dysfunction. Molecular chaperones have been studied in relation to de novo protein folding, but there is increasing awareness that chaperone function is required for the regulation of protein dynamics when functioning physiologically as an isolated moiety or part of a protein complex. This book will introduce both important concepts of folding machineries and give examples of the biological relevance of further chaperone functions.
  • 2011 Springer
    Chang, Rowen J. Y.; Ventura, Salvador.
    1. Oxidative folding: coupling conformational folding and disulfide formation -- 2. The case of oxidative folding of ribonuclease A: factors impacting fold maturation of ER-processed proteins -- 3. Cystine knot folding in cyclotides -- 4. In vitro folding of single/double chain insulins and related proteins -- 5. Unfolding and refolding of disulfide proteins using the method disulfide scrambling -- 6. Oxidative protein folding with small molecules -- 7. Protein disulfide isomerase and the catalysis of oxidative protein folding -- 8. Allosteric disulfide bonds -- 9. The problem of expression of multidisulfide bonded recombinant proteins in E. coli -- 10. NMR-spectroscopic investigation of disulfide dynamics in unfolded states of proteins -- 11. A half-century of oxidative folding and protein disulfphide formation -- Index.
  • 2009 Springer
    Maiese, Kenneth.
    FOXO1, T-cell trafficking and immune responses / Florent Carrette, Stéphanie Fabre and Georges Bismuth -- FOXP3 and its role in the immune system / Chang H. Kim -- Molecular regulation of cellular immunity by FOXP3 / Alicia N. McMurchy ... [et al.] -- The biology of FOXP3 : a key player in immune suppression during infections, autoimmune diseases and cancer / Frances Mercer and Derya Unutmaz -- The cooperative roles of FOXC1 and FOXC2 in cardiovascular development / Tsutomu Kume -- FOXO proteins and cardiac pathology / Albert Wong and Elizabeth A. Woodcock -- The forkhead transcription factors play important roles in vascular pathology and immunology / Xiao-Feng Yang ... [et al.] -- Regulatory T cells, FOXP3 and atherosclerosis / Michal Entin-Meer, Arnon Afek and Jacob George -- FOXP genes, neural development, speech and language disorders / Hiroshi Takahashi, Kaoru Takahashi and Fu-Chin Liu -- Pathophysiological relevance of forkhead transcription factors in brain ischemia / Kohji Fukunaga and Norifumi Shioda -- New insights for FOXO and cell-fate decision in HIV infection and HIV associated neurocognitive disorder / Min Cui ... [et al.] -- FOXP3+ regulatory T-cells in chronic kidney disease : molecular pathways and clinical implications / Pascal Meier -- Transcriptional role of FOXO1 in drug resistance through antioxidant defense systems / Tomoko Goto and Masashi Takano -- FOXP3 expressing regulatory T-cells in allergic disease / Kayhan T. Nouri-Aria -- Human clinical phenotype associated with FOXN1 mutations / Claudio Pignata, Anna Fusco and Stefania Amorosi -- FOXL2 : at the crossroads of female sex determination and ovarian function / Bérénice A. Benayoun ... [et al.] -- FOXO transcription factors : from cell fate decisions to regulation of human female reproduction / Jan J. Brosens, Miranda S.C. Wilson and Eric W.-F. Lam -- The "O" class : crafting clinical care with FOXO transcription factors / Kenneth Maiese ... [et al.].
    Also available: Print – 2009
  • 2013 Springer
    Banerji, Anirban.
    Introduction -- Characterization of Protein-Protein Interfaces, Considering Surface-Roughness and Local Shape -- Adhesion on Protein (and Other Rough Biomolecular) Surfaces.
  • 2013 Springer
    Banerji, Anirban.
    Introduction -- Characterization of Protein-Protein Interfaces, Considering Surface-Roughness and Local Shape -- Adhesion on Protein (and Other Rough Biomolecular) Surfaces.
  • 2006 Springer
    Sonntag, C. von.
  • 2012 Springer
    Barciszewski, Jan; Erdmann, V. A.
    RNAissance / Juergen Brosius -- Nucleic Acids as Therapeutics / Saraswat Pushpendra, Pareek Arvind and Bhandari Anil -- Thio Effects as a Tool for Mechanistic Studies of the Cleavage of RNA Phosphodiester Bonds: The Chemical Basis / Mikko Ora, Tuomas Lönnberg and Harri Lönnberg -- Off-Target Effects and Safety Aspects of Phosphorothioate Oligonucleotides / Martina Stessl, Christian R. Noe and Johannes Winkler -- Oligonucleotide Conjugates: Rationale, Synthesis, and Applications / Yashveer Singh, Pierre Murat, Nicolas Spinelli and Eric Defrancq -- CRISPR: A Bacterial Immunity System Based on Small RNAs / Rolf Wagner and Ümit Pul -- Antisense Technology: From Unique Laboratory Tool to Novel Anticancer Treatments / Christine Di Cresce, Colin Way, Mateusz Rytelewski, Saman Maleki Vareki and Supritha Nilam, et al. -- Antisense-Mediated Reduction of Eukaryotic Noncoding RNAs / Xue-hai Liang, Timothy A. Vickers and Stanley T. Crooke -- Antisense Oligonucleotides in the Treatment of Malignant Gliomas / Gerardo Caruso, Mariella Caffo, Giuseppe Raudino, Federica Raudino and Mario Venza, et al. -- Natural Antisense Transcripts Mediate Regulation of Gene Expression / Marco Magistri and Mohammad Ali Faghihi -- Activation and Deactivation of Antisense and RNA Interference Function with Light / Jeane M. Govan and Alexander Deiters -- Ribozymes as Molecular Biology Reagents / Sanchita Bhadra, Arti Pothukuchy and Andrew Ellington -- RNA Technologies for Mitochondrial Genetics / André Dietrich, Katarzyna Rolle, Marta Gabryelska, Eliza Wyszko and Romain Val, et al. -- RNA-Based Therapies for Inherited Metabolic Diseases / Belén Pérez, Magdalena Ugarte and Lourdes R. Desviat -- RNA-Cleaving DNA Enzymes and Their Potential Therapeutic Applications as Antibacterial and Antiviral Agents / P. I. Pradeepkumar and Claudia Höbartner -- Hammerhead Ribozymes Against Virus and Viroid RNAs / Alberto Carbonell, Ricardo Flores and Selma Gago -- Suppression of Hepatitis C Viral Genome Replication with RNA-Cleaving Deoxyribozyme / Dal-Hee Min and Dong-Eun Kim -- Design of Synthetic shRNAs for Targeting Hepatitis C: A New Approach to Antiviral Therapeutics / Brian H. Johnston and Qing Ge -- The Diverse Active Sites in Splicing, Debranching, and MicroRNA Processing Around RNA Phosphodiester Bonds / Sourav Kumar Dey, Eduardo Paredes, Molly Evans and Subha R. Das -- MicroRNAs and Their Antagonists as Novel Therapeutics / Jeppe Vinther, Jakob Lewin Rukov and Noam Shomron -- microRNAs in Human Diseases and Viral Infections / Alessandra Mescalchin and Tobias Restle -- Dysregulation of MicroRNA Expression and Human Diseases? / To-Ha Thai -- Noncoding RNAs: Identification of Cancer-Associated MicroRNAs / Muller Fabbri and George A. Calin -- Targeting Non-coding RNAs for Cancer Therapy / Satya K. Kota and Savithri Balasubramanian -- MicroRNA Pathways in Drosophila / Geetanjali Chawla and Nicholas S. Sokol -- Viroids: The Smallest Known Infectious Agents Cause Accumulation of Viroid-Specific Small RNAs / Jaroslav Matoušek, Detlev Riesner and Gerhard Steger.
  • 2011
    Chuan, Peiying; Dunn, Alexander Robert; Harbury, Pehr A. B.; Herschlag, Daniel; Spudich, James A.
    Myosins are a superfamily of molecular motors that couple the chemical energy derived form adenosine triphosphate (ATP) hydrolysis to precise mechanical movements along the filamentous cytoskeletal protein actin. There exist more than 20 distinct classes of myosins within this superfamily, and their specific biophysical and biochemical properties likely allow them to function accordingly in vivo. My research has focused on understanding the how the structural and kinetic characteristics of a particular myosin, myosin VI, relate to its proposed trafficking and tethering functions. In the first section of this dissertation, I perform single molecule studies on the contribution of an unexpected structural domain in the myosin VI tail to the efficiency of processive stepping, especially against external forces. I additionally use optical trapping to examine the load-dependent kinetics of the motor in the second section, and propose that external load in vivo likely regulates myosin VI's dual functions as cellular transporter and cytoskeletal anchor. The specific cellular cargoes as well as oligomerization states of different myosins are defined by their tail domain, which is the most divergent structural region among myosin classes. Myosin VI, shown to be monomeric when purified in its native form, likely dimerizes upon cargo binding. As most in vitro work on myosin VI so far have been performed on a truncated, artificially forced myosin VI dimer, I discuss in the third section steps taken toward achieving a more native, biochemically reconstituted full-length myosin VI molecule bound to its bona fide cargo. Having gained a better understanding of the biophysics, kinetics and biochemistry of myosin at the single molecule level, I moved on to studying myosin function at the cellular level. In the forth section of this dissertation, I examine the effects of a familial hypertrophic cardiomyopathy (FHC) mutation, R403Q in cardiac myosin, on the contractile function of single mouse cardiomyocytes. I find that the functional effects of the R403Q mutation in the diseased FHC heart are manifest at the level of cardiomyocytes as well, suggesting that these effects are cell autonomous. From these studies, we can better appreciate the structural and kinetic properties of myosin VI that make it uniquely suited for its functions. This knowledge is also beneficial in aiding the understanding of other molecular motors that may employ similar mechanisms. We are now also aware of the cell-autonomy of particular dysfunctions exhibited by diseased R403Q FHC hearts, and are better poised to understand the complex disorder as a whole.
  • 2006 Springer
    2006 MyiLibrary
    Cheng, Jing; Xing, Wan-Li.
  • 2007 Springer
    Leung, Po Sing.
  • 2011 Springer
    Nettleship, Joanne E.; Owens, Raymond J.
    Glycoproteomics in health and disease / Weston B. Struwe, Eoin F.J. Cosgrave, Jennifer C. Byrne, Radka Saldova, and Pauline M. Rudd -- Glyco-engineering of Fc Glycans to enhance the biological functions of therapeutic IgGs / T. Shantha Raju, David M. Knight, and Robert E. Jordan -- Bioinformatics databases and applications available for glycobiology and glycomics / René Ranzinger, Kai Maass, and Thomas Lütteke -- Lectin microarrays: Simple tools for the analysis of complex glycans / Lakshmi Krishnamoorthy and Lara K. Mahal -- The application of high throughput mass spectrometry to the analysis of glycoproteins / Sasha Singh, Morten Thaysen Andersen, and Judith Jebanathirajah Steen -- Solutions to the glycosylation problem for low- and high-throughput structural glycoproteomics / Simon J. Davis and Max Crispin -- Role of glycoproteins in virus-human cell interactions / Thomas A. Bowden and Elizabeth E. Fry.
  • 2010 ScienceDirect
    Fukuda, Minoru.
    Also available: Print – 2010
  • 2006 CRCnetBASE
    Feng, Li; Prestwich, Glenn D.
  • 2014 Springer
    Abmayr, Susan M.; Workman, Jerry L.
    Chromatin is the combination of DNA and proteins that make up the genetic material of chromosomes. It is essential for packaging DNA, regulation of gene expression, DNA replication and repair. The audience for this book includes newly established scientists, graduate students and scientists seeking detailed overviews of various aspects of chromatin structure and function to gain entry into this field. The chapters in this book give a strong, updated groundwork about the fundamentals of chromatin which are essential to understand epigenetics. Some the fundamentals that this book covers include the structure and biochemistry of chromatin and the enzyme complexes that manage it.
  • 2007 Springer
    Buxbaum, Engelbert.
    Pt. I. Protein structure. Amino acids ; Protein structure ; Purification and characterisation of proteins ; Protein sequencing -- Pt. II. Enzymes. Enzymes are biocatalysts ; Enzyme kinetics ; Haemoglobin and myoglobin: co-operativity ; Enzyme kinetics: special cases -- Pt. III. Special proteins. Prion proteins and prion diseases ; Immunoproteins ; Cell skeleton ; Motor proteins and movement ; Cell-cell interactions ; Aiding in folding: molecular chaperones and chaperonins -- Pt. IV. Membrane transport. Transport of proteins into mitochondria ; Intracellular protein transport ; Transport of solutes across membranes -- Appendix. A.1. Solutions for the exercise ; A.2. Short biographies of scientists mentioned in this book ; A.3. List of symbols ; A.4. Greek alphabet ; A.5. The genetic code ; A.6. Acronyms.
  • 2012 Springer
    Fuxreiter, Monika; Tompa, Peter.
    Fuzzy complexes : a more stochastic view of protein function / Monika Fuxreiter and Peter Tompa -- Dynamic fuzziness during linker histone action / Steven J. McBryant and Jeffrey C. Hansen -- Intrinsic protein flexibility in regulation of cell proliferation : advantages for signaling and opportunities for novel therapeutics / Ariele Viacava Follis, Charles A. Galea, and Richard W. Kriwacki -- Interplay between protein order, disorder and oligomericity in receptor signaling / Alexander B. Sigalov -- Consequences of fuzziness in the NFB/IB interaction / Elizabeth A. Komives -- Roles for intrinsic disorder and ̂fuzziness in generating context specific function in ultrabithorax : a hox transcription factor / Sarah E. Bondos and Hao Ching Hsiao -- Molecular recognition by the EWS transcriptional activation domain / Kevin A.W. Lee -- The measles virus NTAILXD complex : an illustrative example of fuzziness / Sonia Longhi -- Fuzziness in the core of the human pathogenic viruses HCV and HIV / Roland Ivanyi Nagy and Jean Luc Darlix -- Structural disorder and protein elasticity / Sarah Rauscher and Røgis Pomès -- "Fuzziness" in the cellular interactome: a historical perspective / G. Rickey Welch.
    Also available: Print – 2012
  • 2013 ScienceDirect
    Conn, P. Michael.
    The protein local optimization program and g-protein-coupled receptors:loop restoration and applications to homology modeling -- Modeling active GPCR conformations -- Molecular modeling of adenosine receptors -- Predicting g-protein-coupled receptors families using differet physiochemical properties and pseudo amino acid composition -- Computational design and experimental characterization of GPCR segment models -- G-protein-coupled receptor interaction with small GTPases -- Quantification of receptor tyrosine kinase activiation and transactivation by G-protein-coupled receptors using spatial intensity distribution analysis (SplDA) -- NMR modeling for detection of small molecule binding to RGS4 -- Group II activators of g-protein signaling: monitoring the interaction of G[alpha] with G-protein motif in the intact cell -- Homogenous time-resolved fluorescence assay to probe folded G protein-coupled receptors -- cAMP biosensors applied in molecular pharmacological studies of G protein-coupled receptors -- Rhodospin-lipid intereactions studied by NMR -- Biasing the parathyroid hormone receptor: relating in vitro ligand efficacy to in vivo biological activity -- Application of Monte Carlo-based receptor ensemble docking to virtual screening for GPCR ligands -- From heptahelical bundle to hits from the haystack: structure-based virtual screening for GPCR ligands -- Kinetics and dynamics in the G protein-coupled receptor signaling cascade -- Magic angle spinning nuclear magnetic resonance spectroscopy fo G protein-coupled receptors -- Posttranslation modification of G protein-coupled receptor in relationship to biased agonism -- Protein S-nitrosylation measurement.
  • 2013 ScienceDirect
    Conn, P. Michael.
    This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain. This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain.
  • 2013 ScienceDirect
    Conn, P. Michael.
    Section 1. GPCR trafficking. Therapeutic rescue of misfolded/mistrafficked mutants : automation-friendly high-throughput assays for identification of pharmacoperone drugs of GPCRs / David C. Smithson, Jo Ann Janovick, and P. Michael Conn ; Trafficking of the follitropin receptor / Alfredo Ulloa-Aguirre, James A. Dias, George Bousfield, Ilpo Huhtaniemi, and Eric Reiter ; Single-molecule imaging technique to study the dynamic regulation of GPCR function at the plasma membrane / B.E. Snaar-Jagalska, A. Cambi, T. Schmidt, and S. de Keijzer ; GPCR oligomerization and receptor trafficking / Richard J. Ward, Tian-Rui Xu, and Graeme Milligan ; [Beta]-arrestins and G protein-coupled receptor trafficking / Dong Soo Kang, Xufan Tian, and Jeffrey L. Benovic ; Tracking cell surface mobility of GPCRs using [alpha]-bungarotoxin-linked fluorophores / Saad Hannan, Megan E. Wilkins, Philip Thomas, and Trevor G. Smart ; Regulatory mechanism of G protein-coupled receptor trafficking to the plasma membrane : a role for mRNA localization / Kusumam Joseph, Eleanor K. Spicer, and Baby G. Tholanikunnel ; Dissecting trafficking and signaling of atypical chemokine receptors / Elena Borroni, Cinzia Cancellieri, Massimo Locati, and Raffaella Bonecchi -- Section 2. Trafficking motifs. Systematic and quantitative analysis of G protein-coupled receptor trafficking motifs / Carl M. Hurt, Vincent K. Ho, and Timothy Angelotti ; Identification of endoplasmic reticulum export motifs for G protein-coupled receptors / Guangyu Wu ; Amino acid residues of G-protein-coupled receptors critical for endoplasmic reticulum export and trafficking / Motonao Nakamura, Daisuke Yasuda, Nobuaki Hirota, Teruyasu Yamamoto, Satoshi Yamaguchi, Takao Shimizu, and Teruyuki Nagamune -- Section 3. GPCR oligomerization. G-protein-coupled heteromers : regulation in disease / Ivone Gomes, Achla Gupta, and Lakshmi A. Devi ; Hetero-oligomerization and specificity changes of G protein-coupled purinergic receptors : novel insight into diversification of signal transduction / Tokiko Suzuki, Kazunori Namba, Natsumi Mizuno, and Hiroyasu Nakata ; Bimolecular fluorescence complementation analysis of G protein-coupled receptor dimerization in living cells / Karin F.K. Ejendal, Jason M. Conley, Chang-Deng Hu, and Val J. Watts ; G protein-coupled receptor heterodimerization in the brain / Dasiel O. Borroto-Escuela, Wilber Romero-Fernandez, Pere Garriga, Francisco Ciruela, Manuel Narvaez, Alexander O. Tarakanov, Miklós Palkovits, Luigi F. Agnati, and Kjell Fuxe ; Experimental strategies for studying G protein-coupled receptor homo- and heteromerization with radioligand binding and signal transduction methods / Roberto Maggio, Cristina Rocchi, and Marco Scarselli ; Analysis of GPCR dimerization using acceptor photobleaching resonance energy transfer techniques / Marta Busnelli, Mario Mauri, Marco Parenti, and Bice Chini ; Techniques for the discovery of GPCR-associated protein complexes / Avais Daulat, Pascal Maurice, and Ralf Jockers ; Expression, purification, and analysis of G-protein-coupled receptor kinases / Rachel Sterne-Marr, Alison I. Baillargeon, Kevin R. Michalski, and John J.G. Tesmer ; Modern methods to investigate the oligomerization of glycoprotein hormone receptors (TSHR, LHR, FSHR) / Marco Bonomi and Luca Persani.
  • 2002 ScienceDirect
    Hildebrandt, John D.; Iyengar, Ravi.
    Also available: Print – 2002
  • 2002 ScienceDirect
    Hildebrandt, John D.; Iyengar, Ravi.
    Also available: Print – 2002
  • 2002 ScienceDirect
    Hildebrandt, John D.; Iyengar, Ravi.
    Also available: Print – 2002
  • 2014 Springer Protocols
    Stevens, Craig W.
    G protein-coupled receptors : research and methods in the post-genomic era / Craig W. Stevens -- Examining the dynamic evolution of G protein-coupled receptors / Claudia Stäubert, Diana Le Duc, and Torsten Schöneberg -- Functional properties of virus-encoded and virus-regulated G protein-coupled receptors / Katja Spiess and Mette M. Rosenkilde -- Discovery and function of the very large G protein-coupled receptor / Perrin C. White and D. Randy McMillan -- Functional evolution of opioid family G protein-coupled receptors / Eyal Vardy, Craig W. Stevens, and Bryan L. Roth -- Investigation of G protein-coupled receptor function and regulation using antisense / Jonathon Willets and Craig Nash -- Signalosome profiling reveals allosteric interactions between G protein-coupled receptors / Katrin Altosaar ... [et al.] -- Use of optogenetic approaches to control intracellular signaling of G protein-coupled receptors / Olivia A. Masseck, Melanie D. Mark, and Stefan Herlitze -- Viral infection for G protein-coupled receptor expression in eukaryotic cells / Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi -- Using in vitro mutagenesis to characterize structure- function relationships in G protein-coupled receptors / Gregory W. Sawyer and Frederick J. Ehlert -- CRE Luc Mouse model for bioimaging ligand activation of G protein-coupled receptors / H. Gregory Polites and Kyriakos D. Economides -- Inducing conformational changes in G protein-coupled receptors by domain coupling / Hamiyet Unal and Sadashiva S. Karnik -- Rescue of defective G protein-coupled receptor function by intermolecular cooperation / Ashutosh Trehan ... [et al.] -- Design of super-arrestins for gene therapy of diseases associated with excessive signaling of G protein-coupled receptors / Vsevolod V. Gurevich and Eugenia V. Gurevich -- Importance of G protein-coupled receptor genetics in clinical medicine / Ramakrishna Devaki -- Pharmacogenomics of G protein-coupled receptor signaling and other pathways in essential hypertension / Pedro A. Jose ... [et al.] -- Exon sequencing of G protein-coupled receptor genes and perspectives for disease treatment / Angeliki Karamitri and Ralf Jockers -- Gene therapy using G protein-coupled receptors for the treatment of cardiovascular disease / Dario Leosco ... [et al.] -- Novel mechanisms of G protein-coupled receptor oligomer and ion channel Iiteractions in nociception / Ajay S. Yekkirala -- Discovery and characterization of leucine-rich repeat-containing G protein-coupled receptors / Marie-Isabelle Garcia, Valeria Fernandez-Vallone, and Gilbert Vassart -- Role of G protein-coupled receptors in adult neurogenesis / Katie M. Collette, Van A. Doze, and Dianne M. Perez.
  • 2014 Springer
    Filizola, Marta.
    G protein-coupled receptors (GPCRs) are heptahelical transmembrane receptors that convert extra-cellular stimuli into intra-cellular signaling, and ultimately into biological responses. Since GPCRs are natural targets for approximately 40% of all modern medicines, it is not surprising that they have been the subject of intense research. Notwithstanding the amount of data generated over the years, discovering ligands of these receptors with optimal therapeutic properties is not straightforward and has certainly been hampered for years by the lack of high-resolution structural information about these receptors.--Back cover.
    Also available: Print – 2014
  • 2007 Springer
    Enna, S. J.; Möhler, Hanns.
  • 2010 Springer
    Hökfelt, T.
    Galanin : a multitalented neuropeptide / T. Hökfelt and K. Tatemoto -- Galanin, galanin receptors, and drug targets / K. Mitsukawa, X. Lu, and T. Bartfai -- Galanin acts as a trophic factor to the central and peripheral nervous systems / S-A. Hobson ... [et al.] -- Galanin and spinal pain mechanisms : past, present, and future / Xiao-Jun Xu, Tomas Hökfelt, and Zsuzsanna Wiesenfeld-Hallin -- Galanin family of peptides in skin function / J.W. Bauer ... [et al.] -- Galanin in glia : expression and potential roles in the CNS / Helmut Butzkueven and Andrew L. Gundlach -- Galanin and the neuroendocrine axes / Istvan Merchenthaler -- Galanin and consummatory behavior : special relationship with dietary fat, alcohol, and circulating lipids / Jessica R. Barson, Irene Morganstern, and Sarah F. Leibowitz -- Neurochemical modulation of central cardiovascular control : the integrative role of galanin / Zaida Díaz Cabiale ... [et al.] -- Galanin impairs cognitive abilities in rodents : relevance to Alzheimer's disease / J.N. Crawley -- Neuroprotective role for galanin in Alzheimer's disease / Scott E. Counts ... [et al.] -- Galanin, galanin receptor subtypes, and depression-like behaviour / Eugenia Kuteeva ... [et al.] -- Galanin and epilepsy / J.T. Lerner, R. Sankar, and A.M. Mazarati -- Galanin and addiction / Marina R. Picciotto -- Galanin systems and ischemia : peptide and receptor plasticity in neurons and oligodendroglial precursors / Pei-Juan Shen and Andrew L. Gundlach -- The galanin system in cancer / I. Rauch and Barbara Kofler -- Galanin systems in non-mammalian vertebrates with special focus on fishes / Elsie Tachie Mensah, Helene Volkoff, and Suraj Unniappan -- Galanin-like peptide : neural regulator of energy homeostasis and reproduction / Catherine B. Lawrence and Gregory S. Fraley.
  • 2015 Springer Protocols
    Cummings, Richard D.; Stowell, Sean R.
    Evolving mechanistic insights into galectin functions / Connie M. Arthur [and three others] -- Cloning, expression, and purification of galectins for in vitro studies / Paul A. Poland, Carol L. Kinlough, and Rebecca P. Hughey -- Alkylation of galectin-1 with iodoacetamide and mass spectrometric mapping of the sites of incorporation / Sean R. Stowell [and three others] -- Evaluation of galectin binding by frontal affinity chromatography (FAC) / Jun Iwaki and Jun Hirabayashi -- Probing lectin-mucin interactions by isothermal titration microcalorimetry / Tarun K. Dam and C. Fred Brewer -- Examination of whole cell galectin binding by solid phase and flow cytometric analysis / Anne Leppänen, Connie M. Arthur, Sean R. Stowell, and Richard D. Cummings -- Evaluation of galectin binding by surface plasmon resonance / Padmaja Mehta-D'souza -- Examining galectin binding specificity using glycan microarrays / Connie M. Arthur [and seven others] -- Methods for assessing the effects of galectins on leukocyte trafficking / Beatrice R. Gittens, Rachael D. Wright, and Dianne Cooper -- Examination of the role of galectins in plasma cell differentiation / Chih-Ming Tsai and Kuo-I Lin -- Examination of galectin-induced lattice formation on early B-cell development / Stéphane J.C. Mancini [and five others] -- Detection of phosphatidylserine exposure on leukocytes following treatment with human galectins / Connie M. Arthur [and five others] -- Examination of galectins in phagocytosis / Huan-Yuan Chen [and four others] -- Assessing the roles of galectins in regulating dendritic cell migration through extracellular matrix and across lymphatic endothelial cells / Sandra Thiemann, Jeanette H. Man, and Linda G. Baum -- Examination of the role of galectins in intestinal inflammation / Atsushi Nishida, Cindy W. Lau, and Atsushi Mizoguchi -- Study of galectins in tumor immunity : strategies and methods / Juan P. Cerliani [and seven others] -- Galectins in the regulation of platelet biology / Maria A. Romaniuk, Gabriel A. Rabinovich, and Mirta Schattner -- Examination of the role of galectins and galectin inhibitors in endothelial cell biology / Iris A. E. Schulkens [and four others] -- Regulation of galectins by hypoxia and their relevance in angiogenesis : strategies and methods / Mariana Salatino [and ten others] -- Examination of the role of galectins during in vivo angiogenesis using the chick chorioallantoic membrane assay / Esther A. Kleibeuker [and four others] -- Examination of the role of galectins in cell migration and re-epithelialization of wounds / Zhiyi Cao [and three others] -- Manipulating galectin expression in Zebrafish (Danio rerio) / Chiguang Feng [and seven others] -- Examination of galectin localization using confocal microscopy / Daniel Giuliano Cerri [and six others] -- Examination of the regulation of galectin-3 expression in cancer / Hafiz Ahmed and Gargi Bandyopadhyaya -- Evaluation of the role of galectins in parasite immunity / Sarah Preston [and four others] -- Effect of galectins on viral transmission / Michel Ouellet [and three others] -- Evaluation of the bactericidal activity of galectins / Connie M. Arthur, Richard D. Cummings, and Sean R. Stowell -- Examination of the role of galectins in pre-mRNA splicing / Ronald J. Patterson [and three others] -- Analysis of the intracellular role of galectins in cell growth and apoptosis / Daniel K. Hsu [and three others] -- Nucleocytoplasmic shuttling of galectin-3 / Eric J. Arnoys, Cheri M. Ackerman, and John L. Wang.
  • 2013 Springer
    Castellano, Immacolata; Merlino, Antonello.
    Gamma-Glutamyl Transpeptidases (g-GTs) are members of the N-terminal nucleophile hydrolase superfamily, enzymes that cleave the g-glutamyl amide bond of glutathione to liberate cysteinylglycine. The released g-glutamyl group can be transferred to water (hydrolysis) or to amino acids or short peptides (transpeptidation). g-GT plays a key role in the gamma glutamyl cycle by regulating the cellular levels of the antioxidant glutathione, hence it is a critical enzyme in maintaining cellular redox homeostasis.g-GT is upregulated during inflammation and in several human tumors, and it is involved in many physiological disorders related to oxidative stress, such as Parkinson's disease and diabetes. Furthermore, this enzyme is used as a marker of liver disease and cancer. This book covers current knowledge about the structure-function relationship of g-GTs and gives information about applications of g-GTs in different fields ranging from clinical biochemistry to biotechnology and biomedicine.
  • 2005 Springer
    Winterhager, Elke.
  • 2013 Springer
    Seidler, Norbert W.
    Also available: Print – 2013
  • 2011 Springer Protocols
    Gevaert, Kris; Vandekerckhove, Joël.
    Mass spectrometry-driven proteomics : an introduction / Kenny Helsens [and others] -- Metabolic labeling of model organisms using heavy nitrogen (15N) / Joost W. Gouw, Bastiaan B.J. Tops, and Jeroen Krijgsveld -- Trypsin-catalyzed oxygen-18 labeling for quantitative proteomics / Wei-Jun Qian [and others] -- ICPL labeling strategies for proteome research / Friedrich Lottspeich and Josef Kellermann -- Quantitative proteome analysis using isobaric peptide termini labeling (IPTL) / Magnus Ø. Arntzen [and others] -- Complete chemical modification of amine and acid functional groups of peptides and small proteins / Casey J. Krusemark [and others] -- Production and use of stable isotope-labeled proteins for absolute quantitative proteomics / Dorothée Lebert [and others] -- Organelle proteomics / Sophie Duclos and Michel Desjardins -- Membrane protein digestion -- comparison of LPI HexaLane with traditional techniques / Ping Sui [and others] -- GeLCMS for in-depth protein characterization and advanced analysis of proteomes / Alicia Lundby and Jesper V. Olsen -- Exploring new proteome space : combining Lys-N proteolytic digestion and strong cation exchange (SCX) separation in peptide-centric MS-driven proteomics / Nadia Taouatas, Shabaz Mohammed, and Albert J.R. Heck -- Quantitation of newly synthesized proteins by pulse labeling with azidohomoalanine / Gertjan Kramer [and others] -- Analytical strategies in mass spectrometry-based phosphoproteomics / Heidi Rosenqvist, Juanying Ye, and Ole N. Jensen -- Protocol on the use of titanium dioxide chromatography for phosphoproteomics / Martijn W.H. Pinkse, Simone Lemeer, and Albert J.R. Heck -- Positional proteomics at the N-Terminus as a means of proteome simplification / Gemma R. Davidson, Stuart D. Armstrong, and Robert J. Beynon -- N-Terminomics : a high-content screen for protease substrates and their cleavage sites / John C. Timmer and Guy S. Salvesen -- Protease specificity profiling by tandem mass spectrometry using proteome-derived peptide libraries / Oliver Schilling, Ulrich auf dem Keller, and Christopher M. Overall -- Identification of proteolytic products and natural protein N-Termini by terminal amine isotopic labeling of substrates (TAILS) / Alain Doucet [and others] -- Lectins as tools to select for glycosylated proteins / Els J.M. Van Damme -- Strong cation exchange chromatography for analysis of sialylated glycopeptides / Katharina Lohrig, Albert Sickmann, and Urs Lewandrowski -- Titanium dioxide enrichment of sialic acid-containing glycopeptides / Giuseppe Palmisano, Sara E. Lendal, and Martin R. Larsen -- Chemical De-O-glycosylation of glycoproteins for applications in LC-based proteomics / Franz-Georg Hanisch -- Ubiquitination and degradation of proteins / Yelena Kravtsova-Ivantsiv and Aaron Ciechanover -- Bioinformatics challenges in mass spectrometry-driven proteomics / Lennart Martens -- Case study on the comparison of different software tools for automated quantification of peptides / Niklaas Colaert [and others].
  • 2012 ScienceDirect
    Friedmann, Theodore.
    General principles of retrovirus vector design / Tammy Chang and Jiing-Kuan Yee -- Strategies for retrovirus-based correction of severe, combined immunodeficiency (SCID) / Alain Fischer, Salima Hacein-Bey-Abina, and Marina Cavazzana-Calvo -- Retrovirus and lentivirus vector design and methods of cell conditioning / Samantha Cooray, Steven J. Howe, and Adrian J. Thrasher -- Analysis of the clonal repertoire of gene-corrected cells in gene therapy / Anna Paruzynski ... [et al.] -- Developing novel lentiviral vectors into clinical products / Anna Leath and Kenneth Cornetta -- Lentivirus vectors in [beta]-thalassemia / Emmanuel Payen ... [et al.] -- Gene therapy for chronic granulomatous disease / Elizabeth M. Kang and Harry L. Malech -- Alternative splicing caused by lentiviral integration in the human genome / Arianna Moiani and Fulvio Mavilio -- Genotoxicity assay for gene therapy vectors in tumor prone mice / Eugenio Montini and Daniela Cesana -- Lentiviral hematopoietic cell gene therapy for X-linked adrenoleukodystrophy / Nathalie Cartier ... [et al.] -- Retroviral replicating vectors in cancer / Christopher R. Logg ... [et al.] -- Adeno-associated virus vectorology, manufacturing, and clinical applications / Joshua C. Grieger and R. Jude Samulski -- Gene delivery to the retina : from mouse to man / Jean Bennett, Daniel C. Chung, and Albert Maguire -- Generation of hairpin-based RNAi vectors for biological and therapeutic application / Ryan L. Boudreau and Beverly L. Davidson -- Recombinant adeno-associated viral vector reference standards / Philippe Moullier and Richard O. Snyder -- NIH oversight of human gene transfer research involving retroviral, lentiviral, and adeno-associated virus vectors and the role of the NIH Recombinant DNA Advisory Committee / Marina O'Reilly ... [et al.] -- Regulatory structures for gene therapy medicinal products in the European Union / Bettina Klug ... [et al.].
  • 2007 Springer
    Lankenau, Dirk-Henner.
    From microorganisms to humans, this volume provides an interdisciplinary overview of how genome integrity is maintained. It begins with DNA replication and continues with replicative DNA repair and pleiotropic protein interactions. It also reviews the cellular responses to radiation and genotoxic stress affecting whole genomes.
  • 2010 Springer
    Nasheuer, Heinz Peter.
    Coming full circle: Cyclin-dependent kinases as anti-cancer drug targets / R.P. Fisher -- Core and linker histone modifications involved in the DNA damage response / J.E. Chubb and S. Rea -- Chromatin assembly and signalling the end of DNA repair requires acetylation of histone H3 on lysine 56 / T. Costelloe and N.F. Lowndes -- Structure and function of histone H2AX / D.M. Pinto and A. Flaus -- The initiation step of eukaryotic DNA replication / H. Pospiech, F. Grosse and F.M. Pisani -- Non-coding RNAs: New players in the field of eukaryotic DNA replication / T. Krude -- Function of TopBP1 in Genome Stability / M. Sokka ... [et al.] -- Eukaryotic single-stranded DNA binding proteins: central factors in genome stability / S. Broderick ... [et al.] -- DNA polymerases and mutagenesis in human cancers / E. Crespan, A. Amoroso and G. Maga -- DNA polymerase [eta], a key protein in translesion synthesis in human cells / S. Cruet-Hennequart ... [et al.] -- The mitochondrial DNA polymerase in health and disease / W.C. Copeland -- Centromeres: assembling and propagating epigenetic function / M. Glynn ... [et al.] -- Nucleotide excision repair in higher eukaryotes: mechanism of primary damage recognition in global genome repair / N.I. Rechkunova and O.I. Lavrik -- Nonhomologous DNA end joining (NHEJ) and chromosomal translocations in humans / M.R. Lieber ... [et al.] -- Fluorescence-based quantification of pathway-specific DNA double-strand break repair activities: a powerful method for the analysis of genome destabilizing mechanisms / M. Bohringer and L. Wiesmuller -- Apoptosis: a way to maintain healthy individuals / C. Mondello and A.I. Scovassi -- The use of transgenic mice in cancer and genome stability research / S. Conmy and H.P. Nasheuer.
  • 2011
    Airen, Isoken Omosefe; Covert, Markus; Spormann, Alfred M.; Swartz, James R.
    We set out to develop and apply a high-throughput cell-free protein synthesis (CFPS) platform that provides functional genomics information for a wide variety of open reading frames (ORFs). We then used this information to improve CFPS yields by 4- to 5-fold, depending on the protein product. With the increasing number of completed genome sequences and ongoing sequencing projects, the post-genomic era has ushered in the promise of complete understanding of biological systems. For such a task, the most important set of information is inarguably the knowledge of the function of each gene product. To lead this endeavor of discerning the properties and functions of the entirety of an organism's genes and gene products, the field of functional genomics has emerged. Functional genomics focuses on dynamic cellular aspects, such as gene transcription, translation, and protein-protein interactions, in attempts to understand the relationship between an organism's genome and its phenotype. Thus, the ultimate goal of such studies is to provide a more complete picture of how biological function arises from the hereditary information of a living system. However, despite this clear interest in analyzing the expression and function of gene products, the development of techniques to address the high-throughput needs of functional genomics has been challenging, given the large diversity of protein functions and physiochemical properties, such as molecular weight and hydrophobicity, as well as the varying expression levels of proteins within a cell. In light of these challenges, we developed a sequential CFPS platform, which is capable of characterizing a variety of diverse proteins in the context of the dynamic metabolic networks that exist in vivo. The first round of expression is directed by PCR-generated expression templates (ETs) and creates an array of cell extracts that are individually enriched with a single target gene product. This round of CFPS is terminated by the selective degradation of the linear DNA templates, and a subsequent round of protein expression is initiated by the addition of a plasmid ET for a reporter protein. The array is then screened to identify gene products that enhanced or inhibited the expression and folding of the reporter. With such a method, we expect that the observations will expand our knowledge of both cell-free and in vivo metabolism, as well as identify key factors and reactions that could potentially lead to improved in vitro transcription, translation and protein folding. CFPS systems offer attractive alternatives to conventional fermentation processes used for protein production. Although improvements in CFPS energetics and reaction conditions have greatly enhanced in vitro protein synthesis, we believe that there are still other issues limiting the productivity of the technology. For this reason, identifying targets that could further improve CFPS is desirable. To validate the developed sequential CFPS protocol, we conducted a genome-wide survey of the well-studied bacterium Escherichia coli (E. coli) to identify soluble gene products that influence the in vitro metabolism. With this method, we identified 139 gene products (79 positive and 60 negative effectors) that influenced the cell-free transcription, translation, and protein folding of our three reporter proteins, as well as the energy metabolism and RNA and protein stability in the CFPS system. Encouragingly, most of the observed effects were consistent with the accepted in vivo metabolic functions of the gene products. However, many were not and required subsequent assays and in-depth literature searches to suggest hypotheses for the in vitro activities of the identified gene products. In many cases, the observations illuminated principles and influences that are unknown, lesser known, or secondary functions that were not expected to influence the CFPS performance. The information from the genome-wide survey was then used to guide modifications of the CFPS system to improve the productivity and duration of in vitro protein synthesis, as well as the efficiency of protein folding. First, fifteen positive effectors were produced and supplemented into the expression reactions in various combinations of the effectors in order to identify cooperative interactions that further enhance system performance. Next, we constructed and evaluated four mutant E. coli strains with chromosomal deletions in non-essential genes that encode negative effectors identified by the genomic survey. We also re-optimized the small molecule metabolite environment in the CFPS reactions. Thus, in the improved in vitro expression system, energy generation, translation initiation and elongation, and protein folding were enhanced; the reaction pH was stabilized; the supplies of specific molecular substrates that are essential for protein synthesis were replenished; and mRNA transcripts were stabilized. With this new system, the total, soluble, and active yields of the several diverse proteins were enhanced by 300 to 400%. The functional genomic analysis of E. coli has greatly broadened our understanding of the biology of the organism. And with the use of species-independent translational leaders that can facilitate cell-free expression (Mureev et al., 2009), our sequential CFPS platform can be used for similar genome-wide surveys of most organisms. In this way, the vast wealth of information available in the sequenced genomes will be utilized, and our knowledge of these biological systems will be significantly improved. Furthermore, the forward, or targeted, metabolic engineering strategy that was used to enhance our CFPS system can be applied to the development and/or improvement of most organism-based in vitro protein expression platforms. These targeted metabolic changes will lead to more rapid and more significant enhancements than traditional improvement strategies, as well as bring us closer to a complete understanding of the biological systems.
  • 2010
    Paulsen, Renee Darlene; Cimprich, Karlene; Meyer, Tobias; Wang, Clifford; Wysocka, Joanna.
    Genome instability has long been known to be a hallmark of cancerous cells, but the cellular causes and consequences of such instability are still not fully understood. Mutations, translocations, DNA rearrangements, as well as chromosomal loss can all result in the loss of genomic integrity. To prevent the disruption of cellular homeostasis due to DNA damage accumulation, cells contain pathways to sense and respond to DNA damage including cell cycle checkpoints and numerous DNA repair processes, collectively known as the DNA damage response (DDR). Mutations in many of the genes involved in the DDR are linked to several diseases, including premature aging, neurodegeneration and cancer. These signaling pathways are especially critical during DNA replication when the DNA is unwound and vulnerable to processing. Here, the cell relies on the S-phase checkpoint to sense DNA damage at the sites of replication forks and to facilitate a number of downstream pathways to maintain genomic stability. These processes include blocking further origin firing, facilitating DNA repair, preventing cell cycle progression, and stabilizing stalled replication forks. Here, two genome-wide siRNA screens were employed to identify additional genes involved in genome stabilization by monitoring phosphorylation of the histone variant H2AX, an early mark of DNA damage. The first screen looked at H2AX phosphorylation that occurred simply by individual protein depletion, and the second screen used a low level of a replication inhibitor, aphidicolin, to specifically identify genes that were needed to prevent DNA damage during S-phase, potentially due to the loss of replication fork stabilization mechanisms. While the results from the second screen are still undergoing further characterization, we did discover hundreds of genes whose down-regulation led to elevated levels of H2AX phosphorylation in the absence of any external stress. From this gene set, we identified many gene networks that were significantly enriched amongst our screening hits as well as several intriguing individual genes that were chosen for follow up study. These included genes involved in mRNA processing, the pathology of Charcot-Marie-Tooth syndrome, and the histone methyl transferase protein, Set8.
  • 2014 Springer
    Marko-Varga, György A.
    This book offers a valuable resource that allows students, researchers, educators and the general public to learn about proteomics and genomics. Chromosomes form the basis for our genetic heritage and are the code for protein synthesis. The Human Genome Map was presented in 2002, and the Proteome Sequence Map is currently being created by a global consortia initiative. Proteome and genome building blocks already form the basis of scientific research areas and shape major areas of the pharmaceutical and biomedical industries. The book provides background information on and our current understanding of these gene and protein areas, and explains in detail how cutting-edge science is using these resources to develop new medicines and new diagnostics for patient treatment and care. The book will benefit all students and researchers who need a good understanding of genomics and proteomics within the clinical field. Its content is also suitable for a broad readership, including those not specialized in this field. Dr. Marko-Varga is head of Div. Clinical Protein Science & Imaging at the Biomedical Center, Dept. of Measurement Technology and Industrial Electrical Engineering, Lund University. He's also Professor at the 1st Department of Surgery, Tokyo Medical University, Tokyo, Japan.
  • 2012 ScienceDirect
    Kangawa, Kenji; Kojima, Masayasu.
    History to the discovery of ghrelin / Cyril Y. Bowers -- Orphan GPCRs and methods for identifying their ligands / Morikatsu Yoshida, Mikiya Miyazato, and Kenji Kangawa -- Purification of rat and human ghrelins / Masayasu Kojima, Hiroshi Hosoda, and Kenji Kangawa -- Variety of acyl modifications in mammalian ghrelins / Takanori Ida -- Determination of nonmammalian ghrelin / Hiroyuki Kaiya ... [et al.] -- Morphological analysis of ghrelin neurons in the hypothalamus / Haruaki Kageyama, Fumiko Takenoya, and Seiji Shioda -- High-performance liquid chromatography analysis of hypothalamic ghrelin / Takahiro Sato and Masayasu Kojima -- Standard sample collections for blood ghrelin measurements / Hiroshi Hosoda and Kenji Kangawa -- From ghrelin to ghrelin's O-acyl transferase / Jesus A. Gutierrez ... [et al.] -- Enzymatic characterization of GOAT, ghrelin O-acyltransferase / Hideko Ohgusu, Tomoko Takahashi, and Masayasu Kojima -- The study of ghrelin deacylation enzymes / Motoyasu Satou and Hiroyuki Sugimoto -- Synthesis of ghrelin : chemical synthesis and semisynthesis for large-scale preparation of modified peptides / Tomohiro Makino, Masaru Matsumoto, and Yoshiharu Minamitake -- Ghrelin O-acyltransferase assays and inhibition / Martin S. Taylor ... [et al.] -- Model-based evaluation of growth hormone secretion / Johannes D. Veldhuis and Daniel M. Keenan -- Ghrelin in the control of energy, lipid, and glucose metabolism / Kristy M. Heppner ... [et al.] -- Ghrelin and the vagus nerve / Yukari Date -- Measurement of AMP-activated protein kinase activity and expression in response to ghrelin / Chung Thong ... [et al.] -- Ghrelin and gastrointestinal movement / Naoki Fujitsuka ... [et al.] -- Ghrelin acylation by ingestion of medium-chain fatty acids / Yoshihiro Nishi, Hiroharu Mifune, and Masayasu Kojima -- Islet [beta]-cell ghrelin signaling for inhibition of insulin secretion / Katsuya Dezaki and Toshihiko Yada -- Rikkunshito as a ghrelin enhancer / Hiroshi Takeda ... [et al.] -- Thermogenic characterization of ghrelin receptor null mice / Ligen Lin and Yuxiang Sun -- Transgenic mice overexpressing ghrelin or ghrelin analog / Hiroyuki Ariyasu ... [et al.] -- Therapeutic potential of ghrelin in restricting-type anorexia nervosa / Mari Hotta ... [et al.] -- Clinical application of ghrelin for chronic respiratory diseases / Nobuhiro Matsumoto and Masamitsu Nakazato -- Clinical trial of ghrelin synthesis administration for upper GI surgery / Shuji Takiguchi ... [et al.].
  • 2013 Springer
    Barańska, Jolanta.
    Also available: Print – 2013
  • pt. A-B, 2008. ScienceDirect
    pt. B, 2008 ScienceDirect
    Poole, Robert K.
    Also available: Print – pt. A-B, 2008.
  • Glick, David M.
  • 2015 Springer
    Harris, Charles; Wang, Jen-Chywan.
    This timely volume provides a comprehensive overview of glucocorticoids and their role in regulating many aspects of physiology and their use in the treatment of disease. The book is broken into four sections that begin by giving a general introduction to glucocorticoids and a brief history of the field. The second section will discuss the effects of glucocorticoids on metabolism, while the third section will cover the effects of glucocorticoids on key tissues. The final section will discuss general topics, such as animal models in glucocorticoid research and clinical implications of glucocorticoid research. Featuring chapters from leaders in the field, this volume will be of interest to both researchers and clinicians.
    Also available: Print – 2015
  • 2005 ScienceDirect
    Packer, Lester; Sies, H.
    Also available: Print – 2005
  • 2011 Springer
    Pavão, Mauro S. G.
    Heparan sulfate proteoglycan in inflammation and angiogenesis / Lianchun Wang -- Heparan sulfate proteoglycans in infection / Allison H. Bartlett and Pyong Woo Park -- Glycans in cancer / Lubor Borsig -- Glycosaminoglycans in atherosclerosis and thrombosis / C.P. Vicente, J.A.P. Godoy, and C.C. Werneck -- Glycans in embryonic stem cells / Claire Pickford ... [et al.] -- Heparan sulfate proteoglycans in cancer therapy / Ezeddin Salem Gassar, Sherif A. Ibrahim, and Martin Götte -- Structure and biological activities of glycosaminoglycan analogs from marine invertebrates : new therapeutic agents? / Eliene O. Kozlowski ... [et al.]
    Also available: Print – 2011
  • 2010 ScienceDirect
    Fukuda, Minoru.
    Also available: Print – 2010
  • 2005 Springer
    Axford, John S.
    Also available: Print – 2005
  • 2006 Springer Protocols
    Brockhausen, Inka.
    Gene expression analysis of glycosylation-related genes by real-time polymerase chain reaction / Juan J. Garcia-Vallejo ... [et al.] -- Analysis of the glycodynamics of primary osteoblasts and bone cancer cells / Inka Brockhausen-Schutzbach, Xiaojing Yang, and Mark Harrison -- Micromethods for the characterization of lipid a core and o-antigen lipopolysaccharide / Cristina L. Marolda ... [et al.] -- Assays for a galactosyltransferase involved in the assembly of the O7 antigen repeat unit of escherichia coli / Inka Brockhausen-Schutzbach ... [et al.] -- High-throughput quantitation of metabolically labeled anionic glycoconjugates by scintillation proximity assay utilizing binding to cationic dyes / Karen J. Rees-Milton and Tassos P. Anastassiades -- Quantitative analysis of mucins in mucosal secretions using indirect ELISA / Pablo Argueso and Ilene K. Gipson -- Molecular dissection of the mouse zona pellucida: an electron microscopic perspective utilizing high-resolution colloidal-gold labeling methods / Frederick W. K. Kan -- Soluble adamantly glycosphingolipid analogs as probes of glycosphingolipid function / Clifford Lingwood ... [et al.] -- Diagnosis of Krabbe disease by use of a natural substrate / John W. Callahan and Marie-Anne Skomorowski -- In vitro assays of the functions of calnexin and calreticulin, lectin-chaperones of the endoplasmic reticulum / Breanna S. Ireland, Monika Niggemann, and David B. Williams -- Quantitative measurement of selectin-ligand interactions: assays to identify a sweet pill in a library of carbohydrates / Mark E. Beauharnois, Sriram Neelamegham, and Khushi L. Matta -- Sialic acid-binding specificities of siglecs / Nadine Bock and Soerge Kelm. The application of fluorophore-assisted carbohydrate -- Electrophoresis for the study of the dolichol pyrophosphate-linked oligosaccharides pathway in cell cultures and animal tissues / Ningguo Gao -- Partial purification of mannosylphosphorylundecaprenol synthase from micrococcus luteus: a useful enzyme for the biosynthesis of a variety of mannosylphosphorylpolyisoprenol products / Jeffrey S. Rush and Charles J. Waechter -- Assay of dolichyl-phospho-mannose synthase reconstituted in a lipid matrix / John S. Schutzbach -- O-mannosylation in mammalian cells / Tamao Endo and Hiroshi Manya -- Methods for analysis of unusual forms of o-glycosylation / Aleksandra Nita-Lazar and Robert S. Haltiwanger -- Structural analysis of naturally occurring sialic acids / Johannis P. Kamerling and Gerrit J. Gerwig -- Non-radioactive trans-sialidase screening assay / Silke Schrader and Roland Schauer -- Structural determination of o-glycans by tandem mass spectrometry / Catherine Robbe, Jean-Claude Michalski, and Calliope Capon -- Detailed structural analysis of n-glycans released from glycoproteins in SDS-page gel bands using HPLC combined with exoglycosidase array digestions / Louise Royle ... [et al.] -- Molecular modeling of glycosyltransferases / Anne Imberty ... [et al.] -- Galactoside_2,6-sialyltransferase (st6gal.i) and the sialyl_2,6-galactosyl-linkage in tissues and cell lines / Fabio Dall'Olio, Nadia Malagolini, and Mariella Chiricolo -- Visualizing intracellular distribution and activity of core 2 _(1,6)n-acetylglucosaminyltransferase-I (C2GNT-I) in living cells / Maelle Prorok-Hamon, Sylvie Mathieu, and Assou El-Battari --
  • 2015 Springer Protocols
    Castilho, Alexandra.
    Current approaches to engineering N-linked protein glycosylation in bacteria / Andreas Naegeli and Markus Aebi -- Inverse metabolic engineering for enhanced glycoprotein production in Escherichia coli / Stephen R. P. Jaffé, Benjamin Strutton, Jagroop Pandhal, and Phillip C. Wright -- GlycoSNAP : a high-throughput screening methodology for engineering designer glycosylation enzymes / Anne A. Ollis, Yi Chai, and Matthew P. DeLisa -- Production of glycoproteins with asparagine-linked N-acetylglucosamine in Escherichia coli / Flavio Schwarz and Markus Aebi -- Glyco-engineering O-antigen-based vaccines and eiagnostics in E. coli / Nicolas F. Vozza and Mario F. Feldman -- Progress in yeast glycosylation engineering / Stephen R. Hamilton and Dongxing Zha -- Protein production with a Pichia pastoris OCH1 knockout strain in fed-batch mode / Christoph Gmeiner and Oliver Spadiut -- Engineering the Pichia pastoris N-glycosylation pathway using the GlycoSwitch technology / Bram Laukens, Charlot De Wachter, and Nico Callewaert -- Development of a valuable yeast strain using a novel mutagenesis technique for the effective production of therapeutic glycoproteins / Hiroko Abe and Ken-ichi Nakayama -- An overview and history of glyco-engineering in insect expression systems / Christoph Geisler, Hideaki Mabashi-Asazuma, and Donald L. Jarvis 11 SweetBac : applying multiBac technology towards flexible modification of insect cell glycosylation / Dieter Palmberger and Dubravko Rendic -- Engineering sialic acid synthesis ability in insect cells / Karthik Viswanathan, Someet Narang, and Michael J. Betenbaugh -- Engineering N-glycosylation pathway in insect cells : suppression of [beta]-N-acetylglucosaminidase and expression of [beta]-1,4-galactosyltransferase / Yeon Kyu Kim and Hyung Joon Cha -- N-glyco-engineering in plants : update on strategies and major achievements / Herta Steinkellner and Alexandra Castilho -- Gene targeting for precision glyco-engineering : production of biopharmaceuticals devoid of plant-typical glycosylation in moss bioreactors / Eva L. Decker, Gertrud Wiedemann, and Ralf Reski -- Im"plant"ing of mammalian glycosyltransferase gene into plant suspension-cultured cells using agrobacterium-mediated transformation / Hiroyuki Kajiura and Kazuhito Fujiyama -- Transient glyco-engineering of N. benthamiana aiming at the synthesis of multi-antennary sialylated proteins / Andreas Loos and Alexandra Castilho -- Subcellular targeting of proteins involved in modification of plant N- and O-glycosylation / Martina Dicker, Jennifer Schoberer, Ulrike Vavra, and Richard Strasser -- Assembly of multigene constructs using golden gate cloning / Sylvestre Marillonnet and Stefan Werner -- Strategies for engineering protein N-glycosylation pathways in mammalian cells / Qiong Wang, Mark Stuczynski, Yuan Gao, and Michael J. Betenbaugh -- Glycan remodeling with processing inhibitors and lectin- resistant eukaryotic cells / Veronica T. Chang, Robert A. Spooner, Max Crispin, and Simon J. Davis -- Production of highly sialylated recombinant glycoproteins using Ricinus communis agglutinin-I-resistant CHO glycosylation mutants / John S. Y. Goh, Kah Fai Chan, and Zhiwei Song -- Metabolic glyco-engineering in eukaryotic cells and selected applications / Friedrich Piller, Aline Mongis, and Véronique Piller -- Evaluation of quenching and extraction methods for nucleotide/nucleotide sugar analysis / Katrin Braasch, Carina Villacrés, and Michael Butler -- Chemoenzymatic glyco-engineering of monoclonal antibodies / John P. Giddens and Lai-Xi Wang -- Chemical polysialylation of recombinant human proteins / Ivan V. Smirnov, Ivan I. Vorobiev, Alexey A. Belogurov, Dmitry D. Genkin, Sergey M. Deyev, and Alexander G. Gabibov -- Site-specific glycosylation profiling using liquid chromatography-tandem mass spectrometry (LC-MS) / Clemens Gruber and Friedrich Altmann -- Mass spectrometric analysis of oligo- and polysialic acids / Christina E. Galuska, Kai Maass, and Sebastian P. Galuska -- Isomer-specific analysis of released N-glycans by LC-ESI MS/MS with porous graphitized carbon / Daniel Kolarich, Markus Windwarder, Kathirvel Alagesan, and Friedrich Altmann.
  • 2015 Springer Protocols
    Frank, Martin; Lütteke, Thomas.
    Annotation of glycomics MS and MS/MS spectra using the glycoworkbench software tool -- Glycobase and autogGU : resources for interpreting HPLC-glycan data -- NMR chemical shift prediction of glycans : application of the computer program CASPER in structural analysis -- Handling and conversion of carbohydrate sequence formats and monosaccharide notation -- Bacterial, plant, and fungal carbohydrate structure databases : daily usage -- Using NMR data on GLYCOSCIENCES.de -- Glycomic analysis using KEGG GLYCAN -- GlycomeDB -- Eukaryotic glycosylation : online methods for site prediction on protein sequences -- Analyzing glycan structure synthesis with the glycan pathway predictor (GPP) tool -- Functional network in posttranslational modifications : Glyco-Net in Glycoconjugate Data Bank -- JCGGDB : Japan Consortium for Glycobiology and Glycotechnology Database -- Glycan array data management at Consortium for Functional Glycomics -- Analyzing glycan-binding patterns with the profilepSTMM tool -- Exploring the specificities of glycan-binding proteins using glycan array data and the GlycoSearch software -- Statistical analysis of amino acids in the vicinity of carbohydrate residues performed by GlyVicinity -- Tools to assist determination and validation of carbohydrate 3D structure data -- Glyco3D : a portal for structural glycosciences -- Solution conformation of carbohydrates : a view by using NMR assisted by modeling -- Informing saccharide structural NMR studies with density functional theory calculations -- Energy maps for glycosidic linkage conformations -- Conformational analysis of oligosaccharides and polysaccharides using molecular dynamics simulations -- Molecular dynamics simulations of membrane- and protein-bound glycolipids using GLYCAM -- Lipopolysaccharide membrane building and simulation -- Molecular dynamics simulations of glycoproteins using CHARMM -- Calculating binding free energies for protein-carbohydrate complexes -- Scoring functions for AutoDock -- Structures of glycans bound to receptors from saturation transfer difference (STD) NMR spectroscopy : quantitative analysis by using CORCEMA-ST -- QM/MM methods for studying enzymatic reactions of glycosyltransferases.
  • 2010 ScienceDirect
    Fukuda, Minoru.
    Also available: Print – 2010
  • 2009 Springer Protocols
    Karlsson, Niclas G.; Packer, Nicolle.
    Analysis of N- and O-linked glycans from glycoproteins using MALTI-TOF mass spectrometry / Willy Morelle ... [et al.] -- Infrared multiphoton dissociation mass spectrometry for structural elucidation of oligosaccharides / Bensheng Li ... [et al.] -- Mass spectrometry of N-linked glycans / Parastoo Azadi and Christian Heiss -- Solid-phase permethylation for glycomic analysis / Yehia Mechref, Pilsoo Kang, and Milos V. Novotny -- Method for investigation of oligosaccharides using phenylhydrazine derivatization / Erika Lattová and Hélène Perreault -- Two-dimensional HPLC separation with reverse-phase-nano-LC-MS/MS for the characterization of glycan pools after labeling with 2-aminobenzamide / Manfred Wuhrer, Carolien A.M. Koeleman, and André M. Deelder -- Capillary lectin-affinity electrophoresis for glycan analysis / Kazuaki Kakehi and Mitsuhiro Kinoshita -- Analysis of methylated O-glycan alditols by reversed-phase nanoLC coupled CAD-ESI mass spectrometry / Franz-Georg Hanisch and Stefan Müller -- High-throughput and high-sensitivity nano-LC/MS and MS/MS for O-glycan profiling / Hasse Karlsson ... [et al.] -- Collision-induced dissociation tandem mass spectrometry for structural elucidation of glycans / Bensheng Li ... [et al.] -- The structural elucidation of glycosaminoglycans / Vikas Prabhakar, Ishan Capila, and Ram Sasisekharan -- Labelling heparan sulphate saccharides with chromophore fluorescence and mass tags for HLC and MS separations / Mark Skidmore ... [et al.] -- Small-scale enzymatic digestion of glycoproteins and proteoglycans for analysis of oligosaccharides by LC-MS and FACE gel electrophoresis / Ruby P. Estrella ... [et al.] -- Enrichment strategies for glycopeptides / Shigeyasu Ito, Ko Hayama, and Jun Hirabayashi -- Introductory glycosylation analysis using SDS-PAGE and peptide mass fingerprinting / Nicole Wilson, Raina Simpson, and Catherine Cooper-Liddell -- Characterization of N-linked glycosylation on recombinant glycoproteins produced in Pichia pastoris using ESI-MS and MALDI-TOF / Bing Gong ... [et al.] -- N-glycosylation site analysis of human platelet proteins by hydrazide affinity capturing and LC-MS/MS / Urs Lewandrowski and Albert Sickmann -- LC/MSn for glycoprotein analysis : N-linked glycosylation analysis and peptide sequencing of glycopeptides / Nana Kawasaki, Satsuki Itoh, and Teruhide Yamaguchi -- Detecting the "O-GlcNAcome" : detection, purification, and analysis of O-GlcNAc modified proteins / Natasha E. Zachara -- Preparation of a glycan library using a variety of glycosyltrasferases [i.e. glycosyltransferases] / Hiromi Ito ... [et al.] -- Data mining the PDB for glyco-related data / Thomas Lütteke and Claus-W. von der Lieth -- Saccharide microarrays for high-throughput interrogation of glycan-protein binding interactions / Andrew K. Powell, Zheng-liang Zhi, and Jeremy E. Turnbull -- Glocosaminoglycan characterization methodologies probing biomolecular interactions / Vikas Prabhakar, Ishan Capila, and Ram Sasisekharan -- Development and characterization of antibodies to carbohydrate antigens / Jamie Heimburg-Molinaro and Kate Rittenhouse-Olson -- Inhibition of glcosyltransferase activities as the basis for drug development / John Schutzbach and Inka Brockhausen -- Saturation transfer difference NMR spectroscopy as a technique to investigate protein-carbohydrate interactions in solution / Thomas Haselhorst, Anne-Christin Lamerz, and Mark von Izstein.
  • 2015 Springer Protocols
    Balagurunathan, Kuberan; Desai, Umesh R.; Nakato, Hiroshi.
    Automated Synthesis of Chondroitin Sulfate Oligosaccharides / Chien-Fu Liang, Heung Sik Hahm, and Peter H. Seeberger -- Enzymatic Synthesis of Heparan Sulfate and Heparin / April Joice [and six others] -- Production of Size-Defined Heparosan, Heparan Sulfate, and Heparin Oligosaccharides by Enzymatic Depolymerization / Spencer Brown and Balagurunathan Kuberan -- Chemical Modification of Heparin and Heparosan / Karthik Raman, Balagurunathan Kuberan, and Sailaja Arungundram -- Synthesis of Sulfur Isotope-Labeled Sulfate Donor, 3'-Phosphoadenosine-5'- Phosphosulfate, for Studying Glycosaminoglycan Functions/ Caitlin Mencio [and three others] -- Preparation of Isotope-Enriched Heparan Sulfate Precursors for Structural Biology Studies / Xylophone V. Victor [and three others] -- Synthesis of Glycosaminoglycan Mimetics Through Sulfation of Polyphenols / Rami A. Al-Horani [and three others] -- Synthesis of Selective Inhibitors of Heparan Sulfate and Chondroitin Sulfate Proteoglycan Biosynthesis / Caitlin Mencio [and three others] -- Ascidian (Chordata-Tunicata) Glycosaminoglycans : Extraction, Purification, Biochemical, and Spectroscopic Analysis / Mauro S.G. Pavão -- Human Blood Glycosaminoglycans : Isolation and Analysis / Md. Ferdous Anower-E-Khuda and Koji Kimata -- Chromatographic Molecular Weight Measurements for Heparin, Its Fragments and Fractions, and Other Glycosaminoglycans / Barbara Mulloy and John Hogwood -- Mass Spectrometric Methods for the Analysis of Heparin and Heparan Sulfate / Miroslaw Lech, Ishan Capila, and Ganesh V. Kaundinya -- Validated Capillary Electrophoretic Assays for Disaccharide Composition Analysis of Galactosaminoglycans in Biologic Samples and Drugs/Nutraceuticals / Athanasia P. Asimakopoulou [and five others] -- Fast Screening of Glycosaminoglycan Disaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) : Applications to Biologic Samples and Pharmaceutical Formulations / Evgenia Karousou [and seven others] -- Capillary Electrophoretic Analysis of Isolated Sulfated Polysaccharides to Characterize Pharmaceutical Products / Zachary Shriver and Ram Sasisekharan -- Methods for Measuring Exchangeable Protons in Glycosaminoglycans / Consuelo N. Beecher and Cynthia K. Larive -- Heparan Sulfate Structure : Methods to Study N-Sulfation and NDST Action / Anders Dagälv [and five others] -- Analysis of Hyaluronan Synthase Activity / Davide Vigetti [and three others] -- A Rapid, Nonradioactive Assay for Measuring Heparan Sulfate C-5 Epimerase Activity Using Hydrogen/Deuterium Exchange-Mass Spectrometry / Ponnusamy Babu [and three others] -- Aggrecan : Approaches to Study Biophysical and Biomechanical Properties / Hadi Tavakoli Nia, Christine Ortiz, and Alan Grodzinsky -- Use of Flow Cytometry for Characterization and Fractionation of Cell Populations Based on Their Expression of Heparan Sulfate Epitopes / Rebecca J. Holley [and five others] -- A Transgenic Approach to Live Imaging of Heparan Sulfate Modification Patterns / Matthew Attreed and Hannes E. Bülow -- Informatics Tools to Advance the Biology of Glycosaminoglycans and Proteoglycans / Lewis J. Frey -- Designing "High-Affinity, High-Specificity" Glycosaminoglycan Sequences Through Computerized Modeling / Nehru Viji Sankaranarayanan [and three others] -- Using Isothermal Titration Calorimetry to Determine Thermodynamic Parameters of Protein-Glycosaminoglycan Interactions/ Amit K. Dutta, Jörg Rösgen, and Krishna Rajarathnam -- Characterizing Protein-Glycosaminoglycan Interactions Using Solution NMR Spectroscopy / Prem Raj B. Joseph, Krishna Mohan Poluri, Krishna Mohan Sepuru, and Krishna Rajarathnam -- Glycosaminoglycan-Protein Interaction Studies Using Fluorescence Spectroscopy / Rio S. Boothello, Rami A. Al-Horani, and Umesh R. Desai -- Studying Glycosaminoglycan-Protein Interactions Using Capillary Electrophoresis / Aiye Liang and Umesh R. Desai -- Histochemical Analysis of Heparan Sulfate 3-O-Sulfotransferase Expression in Mouse Brain / Tomio Yabe and Nobuaki Maeda -- Keratan Sulfate : Biosynthesis, Structures, and Biological Functions / Kenji Uchimura -- The Sulfs : Expression, Purification, and Substrate Specificity / Kenji Uchimura -- The Detection of Glycosaminoglycans in Pancreatic Islets and Lymphoid Tissues / Marika Bogdani [and five others] -- Nonradioactive Glycosyltransferase and Sulfotransferase Assay to Study Glycosaminoglycan Biosynthesis / Cheryl M. Ethen [and five others] -- Mapping Proteoglycan Functions with Glycosidases / Mauricio Cortes, Leslie K. Cortes, and Nancy B. Schwartz -- Cell Substrate Patterning with Glycosaminoglycans to Study Their Biological Roles in the Central Nervous System / Tony W. Hsiao [and three others] -- Analyzing the Role of Heparan Sulfate Proteoglycans in Axon Guidance In Vivo in Zebrafish / Fabienne E. Poulain -- Murine Models in the Evaluation of Heparan Sulfate-Based Anticoagulants / David Gailani, Qiufang Cheng, and Ivan S. Ivanov -- Genetic Approaches in the Study of Heparan Sulfate Functions in Drosophila / Masahiko Takemura and Hiroshi Nakato -- Measuring Sulfatase Expression and Invasion in Glioblastoma / Anna Wade [and three others] -- Synthesis and Biomedical Applications of Xylosides / Mausam Kalita [and four others] -- A Strategic Approach to Identification of Selective Inhibitors of Cancer Stem Cells / Nirmita Patel, Somesh Baranwal, and Bhaumik B. Patel -- Analysis of the Heavy-Chain Modification and TSG-6 Activity in Pathological Hyaluronan Matrices / Mark E. Lauer [and three others] -- Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration / Hong Qiu [and three others] -- Glycosaminoglycan Functionalized Nanoparticles Exploit Glycosaminoglycan Functions / James A. Vassie, John M. Whitelock, and Megan S. Lord -- Role of Glycosaminoglycans in Infectious Disease / Akiko Jinno and Pyong Woo Park -- Isolation and Purification of Versican and Analysis of Versican Proteolysis / Simon J. Foulcer, Anthony J. Day, and Suneel S. Apte -- Analysis of Human Hyaluronan Synthase Gene Transcriptional Regulation and Downstream Hyaluronan Cell Surface Receptor Mobility in Myofibroblast Differentiation / Adam C. Midgley and Timothy Bowen.
  • 2015 ScienceDirect
    Ball, Lauren E.; Drake, Richard R.
  • 2013 Springer Protocols
    Brockhausen, Inka.
    Glycan microarray screening assay for glycosyltransferase specificities / W. Peng, C.M. Nycholat and N. Razi -- A fluorescence-based assay for core 1 [beta]3galactosyltransferase (T-synthase) activity / T. Ju and R.D. Cummings -- Structural and biochemical analysis of a bacterial glycosyltransferase / F. Zhu ... [et al.] -- Study of the biological functions of mucin type core 3 O-glycans / S.H. Lee and M. Fukuda -- Generation of anti-sulfated glycan antibodies using sulfotransferase-deficient mice / H. Kawashima -- Fluorescent microscopy as a tool to elucidate dysfunction and mislocalization of golgi glycosyltransferases in COG complex depleted mammalian cells / R.A. Willett, I.D. Pokrovskaya and V.V. Lupashin -- A practical approach to reconstruct evolutionary history of animal sialyltransferases and gain insights into the sequence-function relationships of golgi-glycosyltransferases / D. Petit ... [et al.] -- Fluorescent lectin staining of drosophila embryos and tissues to detect the spatial distribution of glycans during development / E. Tian, L. Zhang and K.G. Ten Hagen -- Photoaffinity labeling of protein O-mannosyltransferases of the PMT1/PMT2 subfamily / M. Loibl and S. Strahl -- Enzymatic analysis of the protein O-glycosyltransferase, rumi, acting toward epidermal growth factor-like (egf) repeats / H. Takeuchi and R.S. Haltiwanger -- Enzymatic characterization of recombinant enzymes of O-glcNAc cycling / E.J. Kim and J.A. Hanover -- Antibodies and activity measurements for the detection of o-glcnac transferase and assay of its substrate, UDP-glcNAc / T. Lefebvre ... [et al.] -- In vitro glycosylation assay for bacterial oligosaccharyltransferases / M.A. Musumeci, M.V. Ielmini and M.F. Feldman -- In vitro UDP-sugar:Undecaprenyl-phosphate sugar-1-phosphate transferase assay and product detection by thin layer chromatography / K.B. Patel and M.A. Valvano -- In vitro O-antigen ligase assay / X. Ruan and M.A. Valvano -- Functional identification of bacterial glucosyltransferase WbdN / Y. Gao, A. Vinnikova and I. Brockhausen -- Methods for the pasteurella glycosaminoglycan synthases: Enzymes that polymerize hyaluronan, chondroitin, or heparosan chains / P.L. Deangelis -- Methods for measuring class i membrane-bound hyaluronan synthase activity / P.H. Weigel, A.J. Padgett-McCue and B.A. Baggenstoss -- Creation and characterization of glycosyltransferase mutants of Trypanosoma brucei / L. Izquierdo, M.L. Guther and M.A. Ferguson -- Mannose-6-phosphate: A regulator of LLO destruction / N. Gao and M.A. Lehrman -- N-acetylglucosaminyltransferase (GnT) assays using fluorescent oligosaccharide acceptor substrates: GnT-III, IV, V, and IX (GnT-Vb) / S. Takamatsu ... [et al.] -- A method for determination of UDP-GlcNAc: GlcNAc[beta]1-6(GlcNAc[beta]1-2)man[alpha]1-r [GlcNAc to man] beta1-4n-acetylglucosaminyltransferase vi activity / T. Taguchi and N. Taniguchi -- In vitro assays of orphan glycosyltransferases and their application to identify notch xylosyltransferases / M.K. Sethi ... [et al.] -- In vitro folding of [beta]-1,4galactosyltransferase and polypeptide-[alpha]-N-acetylgalactosaminyltransferase from the inclusion bodies / B. Ramakrishnan and P.K. Qasba -- An assay for [alpha] 1,6-fucosyltransferase (FUT8) activity based on the HPLC separation of a reaction product with fluorescence detection / H. Ihara ... [et al.] -- Immunodetection of glycosyltransferases in gastrointestinal tissues / J. Gomes and C.A. Reis -- Glycosyltransferases in chemo-enzymatic synthesis of oligosaccharides / B. Tefsen and I. van Die -- Tumor targeting by a carbohydrate ligand-mimicking peptide / S. Hatakeyama ... [et al.] -- Glycoengineering of human cell lines using zinc finger nuclease gene targeting: Simple cells with homogeneous GalNAc O-glycosylation allow isolation of the O-glycoproteome by one-step lectin affinity chromatography / C. Steentoft, E.P. Bennett and H. Clausen -- Generation of monoclonal antibodies to native active human glycosyltransferases / M.B. Vester-Christensen ... [et al.].
  • 2009 Springer
    Morré, D. James; Mollenhauer, Hilton H.
  • 2012 Springer
    Dupré, Denis J.; Hébert, Terence E.; Jockers, Ralf.
    ER-bound steps in the biosynthesis of G protein-coupled receptors / Christian Nanoff and Michael Freissmuth -- Role of chaperones in G Protein coupled receptor signaling complex assembly / Denis J. Dupré, Maha M. Hammad, Patrick Holland and Jaime Wertman -- GPCR oligomerization: contribution to receptor biogenesis / Kathleen Van Craenenbroeck -- The functional size of GPCRs: monomers, dimers or tetramers? / Darlaine Pétrin and Terence E. Hébert -- Regulation of post-Golgi traffic of G protein-coupled receptors / Guangyu Wu -- Regulated GPCR trafficking to the plasma membrane: general issues and the CCR5 chemokine receptor example / Hamasseh Shirvani, Gabriel Gätà and Stefano Marullo -- Regulatory processes governing the cell surface expression of LH and FSH receptors / Deborah L. Segaloff -- Chaperone-mediated assembly of G protein complexes / Barry M. Willardson and Christopher M. Tracy -- Synthesis and assembly of G protein [beta][gamma] dimers: comparison of in vitro and in vivo studies / Jane Dingus and John D. Hildebrandt -- Preferential assembly of G-[alpha][beta][gamma] complexes directed by the [gamma] subunits / Janet D. Robishaw -- G Protein trafficking / Philip B. Wedegaertner -- Differential assembly of GPCR signaling complexes determines signaling specificity / Pascal Maurice, Abla Benleulmi-Chaachoua and Ralf Jockers -- GPCR and voltage-gated calcium channels (VGCC) signaling complexes / Christophe Altier -- Pharmacological chaperones correct misfolded GPCRs and rescue function: protein trafficking as a therapeutic target / Guadalupe Maya-Núñez, Alfredo Ulloa-Aguirre, Jo Ann Janovick and P. Michael Conn.
  • 2007 Springer
    Bourne, Henry R.; Horuk, Richard.
  • 2007
    Status: Not Checked OutLane Catalog Record
  • 2002 Springer Protocols
    Hicks, Barry W.
  • 2006 Wiley
    Chalfie, Martin; Kain, Steven.
    "Green fluorescent protein (GFP), a biological marker cloned from jellyfish, has emerged from virtual obscurity to the forefront of biomolecular research in just a few years. GFP and related fluorescent proteins (FPs) have been intensely studied - the subjects of hundreds of in vivo projects worldwide. These studies have resulted in new applications and published articles, emerging at a breathtaking rate. The success of FPs and their many uses promise to make them some of the most powerful tools in biotechnology, pharmaceutical science, and molecular and cellular biology."
    Also available: Print – 2006
  • 2005 Springer
    Chowen, Julie A.; Varela-Nieto, Isabel.
    Basic physiology of the growth hormone/insulin-like growth factor (IGF)-axis / Laura M. Frago and Julie A. Chowen -- Experimental models for understanding the role of insulin-like growth factor-I and its receptor during development / Jose Luis Trejo, Eva Carro, and Deborah J. Burke -- Insulin-like growth factor binding proteins in development / Josef V. Silha and Liam J. Murphy -- Growth hormone, insulin-like growth factors, and the female reproductive system / Carolyn A. Bondy and Jian Zhou -- Skeletal muscle / Ana M. Fernandez and Derek LeRoith -- Cardiovascular and respiratory systems / Åsa Tivesten and Jörgen Isgaard -- Hematopoiesis / Wilson Savino, Salete Smaniotto, and Mireille Dardenne -- Brain development / Gregory J. Popken ... [et al.] -- Regulation of vertebrate sensory organ development : a scenario for growth hormone and insulin-like growth factors action / Amelia Diaz-Casares ... [et al.] -- Role of insulin-like growth factors in neuronal plasticity and neuroprotection / Ignacio Torres Alemán -- Aging and life span / Holly M. Brown-Borg -- Interactions of insulin-like growth factor-I and estrogen in the brain / Pablo Mendez, Gloria Patricia Cardona-Gomez, and Luis Miguel García-Segura -- Cancer / Adda Grimberg -- Genetic basis of proportional short stature / Ángel Campos-Barros, Karen E. Heath, Jesús Argente -- Future perspectives : from stem cells and IGF biology to the clinic / Yvan Arsenijevic.
  • 2005 ScienceDirect
    Balch, William Edward; Der, Channing J.; Hall, A.
    Also available: Print – 2005
  • 2005 ScienceDirect
    Balch, William Edward; Der, Channing J.; Hall, A.
    Also available: Print – 2005
  • 2013 Springer Protocols
    Krieg, Thomas; Lukowski, Robert.
    NO/cGMP : the past, the present, and the future / Michael Russwurm [and 3 others] -- cGMP-dependent protein kinases (cGK) / Franz Hofmann and Jörg W. Wegener -- Enzyme assays for cGMP hydrolyzing phosphodiesterases / Sergei D. Rybalkin, Thomas R. Hinds, and Joseph A. Beavo -- Radioimmunoassay for the quantification of cGMP levels in cells and tissues / Ronald Jäger [and 5 others] -- Hyperspectral imaging of FRET-based cGMP probes / Thomas C. Rich [and 3 others] -- Visualization of cGMP with cGi biosensors / Martin Thunemann [and 4 others] -- Advances and techniques to measure cGMP in intact cardiomyocytes / Konrad R. Götz and Viacheslav O. Nikolaev -- Real-time monitoring the spatiotemporal dynamics of intracellular cGMP in vascular smooth muscle cells / Kara F. Held and Wolfgang R. Dostmann -- Methods for identification of cGKI substrates / Katharina Salb and Jens Schlossmann -- Approaches for monitoring PKG1[alpha] oxidative activation / Joseph Robert Burgoyne and Philip Eaton -- Analysis of cGMP signaling in adipocytes / Katja Jennissen [and 4 others] -- A genetic strategy for the analysis of individual axon morphologies in cGMP signalling mutant mice / Hannes Schmidt, Gohar Ter-Avetisyan, and Fritz G. Rathjen -- Receptor binding assay for NO-independent activators of soluble guanylate cyclase / Peter M. Schmidt and Johannes-Peter Stasch -- Direct intrathecal drug delivery in mice for detecting in vivo effects of cGMP on pain processing / Ruirui Lu and Achim Schmidtko -- The Geisler method : tracing activity-dependent cGMP plasticity changes upon double detection of mRNA and protein on brain slices / Wibke Singer, Hyun-Soon Geisler, and Marlies Knipper -- Detection of cGMP in the degenerating retina / Stylianos Michalakis [and 3 others].
  • 2009 ScienceDirect
    Burgess, Richard; Deutscher, Murray P.
    Also available: Print – 2009
  • 2010 CRCnetBASE
    Lundblad, Roger L.; Macdonald, Fiona M.
    "Offering a molecular perspective not available 20 years ago, [this edition] provides physical and chemical data on proteins, nucleic acids, lipids, and carbohydrates. Presented in an organized, concise, and simple-to-use format, this popular reference allows quick access to the most frequently used data. Covering a wide range of topics, from classical biochemistry to proteomics and genomics, it also details the properties of commonly used biochemicals, laboratory solvents, and reagents." --excerpts from book jacket.
  • 2010 Springer
    Jain, K. K.
    Technologies for discovery of biomarkers -- Biomarkers and molecular diagnostics -- Biomarkers for drug discovery and development -- Role of biomarkers in health care -- Biomarkers of cancer -- Biomarkers of disorders of the nervous system -- Biomarkers of cardiovascular disorders -- Biomarkers and personalized medicine -- Regulatory issues.
  • 2007 CRCnetBASE
    Langel, Ulo.
  • 2009 ScienceDirect
    Cummings, Richard D.; Pierce, J. Michael.
  • 2005 ScienceDirect
    Ahuja, Satinder; Garfin, David Edward.
  • 2011 Springer Protocols
    Fan, Teresa Whei-Mei; Higashi, Richard M.; Lane, Andrew N.
    Introduction to metabolomics / Teresa Whei-Mei Fan, Andrew N. Lane, and Richard M. Higashi -- Considerations of sample preparation for metabolomics investigation / Teresa Whei-Mei Fan -- Clinical aspects of metabolomics / Michael Bousamra II [and others] -- Structural mass spectrometry for metabolomics / Richard M. Higashi -- Metabolomic applications of inductively coupled plasma-mass spectrometry (ICP-MS) / Rob Henry and Teresa Cassel -- Principles of NMR for applications in metabolomics / Andrew N. Lane -- Novel NMR and MS approaches to metabolomics / Ian A. Lewis [and others] -- Metabolic flux analysis / Tae Hoon Yang -- Introduction to metabolic control analysis (MCA) / Maliackal Poulo Joy [and others] -- Application of tracer-based metabolomics and flux analysis in targeted cancer drug design / Marta Cascante, Vitaly Selivanov, and Antonio Ramos-Montoya -- Noninvasive fluxomics in mammals by nuclear magnetic resonance spectroscopy / Justyna Wolak [and others] -- Compositional analysis of phospholipids by mass spectrometry and phosphorus-31 nuclear magnetic resonance spectroscopy / M. Cecilia Yappert -- HumanCyc pathway-genome database and pathway tools software as tools for imaging and analyzing metabolomics data / Pedro Romero -- Metabolomics-edited transcriptomics analysis (META) / Teresa Whei-Mei Fan.
  • 2006- Wiley
    Messerschmidt, Albrecht.
    Iron -- NIckel -- Manganese -- Cobalt -- Molybdenum/Tungsten -- Copper -- Vanadium -- Zinc -- Calcium -- Magnesium.
  • 2009 CRCnetBASE
    Liu, Dongyou.
    Nucleic acids : structures, functions, and applications / Valentin V. Vlassov, Dimitrii V. Pyshnyi, and Pavel E. Vorobjev -- Isolation of viral DNA from cultures / Larry A. Hanson -- Isolation of viral RNA from cultures / Ling Zhao and Zhen F. Fu -- Preparation of viral samples for direct molecular applications / Grant S. Hansman -- Isolation of bacterial DNA from cultures / Dongyou Liu -- Isolation of bacterial RNA from cultures / Som Subhra Chatterjee and Trinad Chakraborty -- Isolation of plasmids / Armelle Bigot and Alain Charbit -- Preparation of bacterial samples for direct molecular applications / Knut Rudi ... [et al.] -- Isolation of nucleic acids from yeasts / Lakshman P. Samaranayake, Nipuna B. Parahitiyawa, and C. Jayampath Seneviratne -- Isolation of nucleic acids from filamentous fungi / Margaret E. Katz and Brian F. Cheetham -- Preparation of fungal specimens for direct molecular applications / Françoise Irlinger ... [et al.] -- Isolation of nucleic acids from protozoa / Vitaliano A. Cama and Lihua Xiao -- Isolation of nucleic acids from helminthes / Munehiro Okamoto and Akira Ito -- Isolation of nucleic acids from insects / Kun Yan Zhu -- Preparation of parasitic specimens for direct molecular applications / Huw Vaughan Smith and Rosely Angela Bergamin Nichols -- Isolation of nucleic acids from body fluids / Karen Page and Jacqueline Amanda Shaw -- Isolation of nucleic acids from soft tissues / Sergey Kovalenko -- Isolation of nucleic acids from hard tissues / Judith Weidenhofer and Jennifer A. Byrne -- Isolation of DNA from ancient samples / Paula F. Campos, Eske Willerslev, and M. Thomas P. Gilbert -- Preparation of forensic samples for direct molecular applications / Erin K. Hanson and Jack Ballantyne -- Isolation of nucleic acids from plants / Song Weining and Dongyou Liu -- Isolation of megabase-sized DNA fragments from plants / Meiping Zhang, Yaning Li, and Hong-Bin Zhang -- Purification of nucleic acids from gels / Chunfang Zhang and Theo Papakonstantinou.
  • 2005 Wiley
    Hartmann, Roland K.
    Part I. RNA Synthesis - Enzymatic RNA Synthesis, Ligation and Modification -- Chapter 1. Enzymatic RNA Synthesis using Bacteriophage T7 RNA Polymerase / Heike Gruegelsiepe, Astrid Schön, Leif A. Kirsebom and Roland K. Hartmann, p. 3-21 -- Chapter 2. Production of RNAs with Homogeneous 5' and 3' Ends / Mario Mörl, Esther Lizano, Dagmar K. Willkomm and Roland K. Hartmann, p. 22-35 -- Chapter 3. RNA Ligation using T4 DNA Ligase / Mikko J. Frilander and Janne J. Turunen, p. 36-52 -- Chapter 4. T4 RNA Ligase / Tina Persson, Dagmar K. Willkomm and Roland K. Hartmann, p. 53-74 -- Chapter 5. Co- and Post-Transcriptional Incorporation of Specific Modifications Including Photoreactive Groups into RNA Molecules / Nathan H. Zahler and Michael E. Harris, p. 75-85 -- Chapter 6. 3'-Terminal Attachment of Fluorescent Dyes and Biotin / Dagmar K. Willkomm and Roland K. Hartmann, p. 86-94 -- Part I. RNA Synthesis - Chemical RNA Synthesis -- Chapter 7. Chemical RNA Synthesis, Purification and Analysis / Brian S. Sproat, p. 95-111 -- Chapter 8. Modified RNAs as Tools in RNA Biochemistry / Thomas E. Edwards and Snorri T. H. Sigurdsson, p. 112-129 -- Part II. Structure Determination - Molecular Biology Methods -- Chapter 9. Direct Determination of RNA Sequence and Modification by Radiolabeling Methods / Olaf Gimple and Astrid Schön, p. 132-150 -- Chapter 10. Probing RNA Structures with Enzymes and Chemicals In Vitro and In Vivo / Eric Huntzinger, Maria Possedko, Flore Winter, Hervé Moine, Chantal Ehresmann and Pascale Romby, p. 151-171 -- Chapter 11. Study of RNA-Protein Interactions and RNA Structure in Ribonucleoprotein Particles / Virginie Marchand, Annie Mougin, Agnès Méreau and Christiane Branlant, p. 172-204 -- Chapter 12. Terbium(III) Footprinting as a Probe of RNA Structure and Metal-binding Sites / Dinari A. Harris and Nils G. Walter, p. 205-213 -- Chapter 13. Pb²⁺-induced Cleavage of RNA / Leif A. Kirsebom and Jerzy Ciesiolka, p. 214-228 -- Chapter 14. In Vivo Determination of RNA Structure by Dimethylsulfate / Christina Waldsich and Renée Schroeder, p. 229-237 -- Chapter 15. Probing Structure and Binding Sites on RNA by Fenton Cleavage / Gesine Bauer and Christian Berens, p. 238-249 -- Chapter 16. Measuring the Stoichiometry of Magnesium Ions Bound to RNA / A. J. Andrews and Carol Fierke, p. 250-258 -- Chapter 17. Nucleotide Analog Interference Mapping and Suppression: Specific Applications in Studies of RNA Tertiary Structure, Dynamic Helicase Mechanism and RNA-Protein Interactions, p. 259-293 / Olga Fedorova, Marc Boudvillain, Jane Kawaoka and Anna Marie Pyle -- Chapter 18. Nucleotide Analog Interference Mapping: Application to the RNase P System / Simona Cuzic and Roland K. Hartmann, p. 294-318 -- Chapter 19. Identification and Characterization of Metal Ion Binding by Thiophilic Metal Ion Rescue / Eric L. Christian, p. 319-344 -- Chapter 20. Identification of Divalent Metal Ion Binding Sites in RNA/DNA-metabolizing Enzymes by Fe(II)-mediated Hydroxyl Radical Cleavage / Yan-Guo Ren, Niklas Henriksson and Anders Virtanen, p. 345-353 -- Chapter 21. Protein-RNA Crosslinking in Native Ribonucleoprotein Particles / Henning Urlaub, Klaus Hartmuth and Reinhard Lührmann, p. 354-373 -- Chapter 22. Probing RNA Structure by Photoaffinity Crosslinking with 4-Thiouridine and 6-Thioguanosine / Michael E. Harris and Eric L. Christian, p. 374-384 -- Part II. Structure Determination - Biophysical Methods -- Chapter 23. Structural Analysis of RNA and RNA-Protein Complexes by Small-angle X-ray Scattering / Tao Pan and Tobin R. Sosnick, p. 385-397 -- Chapter 24. Temperature-Gradient Gel Electrophoresis of RNA / Detlev Riesner and Gerhard Steger, p. 398-414 -- Chapter 25. UV Melting, Native Gels and RNA Conformation / Andreas Werner, p. 415-427 -- Chapter 26. Sedimentation Analysis of Ribonucleoprotein Complexes / Jan Medenbach, Andrey Damianov, Silke Schreiner and Albrecht Bindereif, p. 428-437 -- Chapter 27. Preparation and Handling of RNA Crystals / Boris François, Aurélie Lescoute-Phillips, Andreas Werner and Benoît Masquida, p. 438-452 -- Part II. Structure Determination - Fluorescence and Single Molecule Studies -- Chapter 28. Fluorescence Labeling of RNA for Single Molecule Studies / Filipp Oesterhelt, Enno Schweinberger and Claus Seidel, p. 453-474 -- Chapter 29. Scanning Force Microscopy and Scanning Force Spectroscopy of RNA / Wolfgang Nellen, p. 475-487 -- Part III. RNA Genomics and Bioinformatics - Fluorescence and Single Molecule Studies -- Chapter 30. Comparative Analysis of RNA Secondary Structure: 6S RNA / James W. Brow and J. Christopher Ellis, p. 490-512 -- Chapter 31. Secondary Structure Prediction / Gerhard Steger, p. 513-535 -- Chapter 32. Modeling the Architecture of Structured RNAs within a Modular and Hierarchical Framework, p. 536-545 / Benoît Masquida and Eric Westhof -- Chapter 33. Modeling Large RNA Assemblies using a Reduced Representation, p. 546-559 / Jason A. Mears, Scott M. Stagg and Stephen C. Harvey -- Chapter 34. Molecular Dynamics Simulations of RNA Systems / Pascal Auffinger and Andrea C. Vaiana, p. 560-576 -- Chapter 35. Seeking RNA Motifs in Genomic Sequences / Matthieu Legendre and Daniel Gautheret, p. 577-594 -- Chapter 36. Approaches to Identify Novel Non-messenger RNAs in Bacteria and to Investigate their Biological Functions: RNA Mining / Jörg Vogel, E. Gerhart and H. Wagner, p. 595-613 -- Chapter 37. Approaches to Identify Novel Non-messenger RNAs in Bacteria and to Investigate their Biological Functions: Functional Analysis of Identified Non-mRNAs / E. Gerhart, H. Wagner and Jörg Vogel, p. 614-642 -- Chapter 38. Experimental RNomics: A Global Approach to Identify Non-coding RNAs in Model Organisms / Alexander Hüttenhofer, p. 643-654 -- Chapter 39. Large-scale Analysis of mRNA Splice Variants by Microarray / Young-Soo Kwon, Hai-Ri Li and Xiang-Dong Fu, p. 655-663 -- Part IV. Analysis of RNA Function 665 - RNA-Protein Interactions in vitro -- Chapter 40. RNA-Protein Interactions in vitro / Steffen Schiffer, Sylvia Rösch, Bettina Späth, Markus Englert, Hildburg Beier and Anita Marchfelder, p. 666-675 -- Chapter 41. Biotin-based Affinity Purification of RNA-Protein Complexes / Zsofia Palfi, Jingyi Hui and Albrecht Bindereif, p. 676-693 -- Chapter 42. Immunoaffinity Purification of Spliceosomal and Small Nuclear Ribonucleoprotein Complexes / Cindy L. Will, Evgeny M. Makarov, Olga V. Makarova and Reinhard Lührmann, p. 694-709 -- Chapter 43. Northwestern Techniques for the Identification of RNA-binding Proteins from cDNA Expression Libraries and the Analysis of RNA-Protein Interactions / Ángel Emilio Martínez De Alba, Michela Alessandra Denti and Martin Tabler, p. 710-728 -- Part IV. Analysis of RNA Function 665 - RNA-Protein Interactions in vivo -- Chapter 44. Fluorescent Detection of Nascent Transcripts and RNA-binding Proteins in Cell Nuclei, p. 729-736 / Jennifer A. Geiger and Karla M. Neugebauer -- Chapter 45. Identification and Characterization of RNA-binding Proteins through Three-hybrid Analysis / Felicia Scott and David R. Engelke, p. 737-754 -- Chapter 46. Analysis of Alternative Splicing In Vivo using Minigenes / Yesheng Tang, Tatyana Novoyatleva, Natalya Benderska, Shivendra Kishore, Alphonse Thanaraj and Stefan Stamm, p. 755-782 -- Part IV. Analysis of RNA Function 665 - SELEX -- Chapter 47. Artificial Selection: Finding Function amongst Randomized Sequences / Ico de Zwart, Catherine Lozupone, Rob Knight, Amanda Birmingham, Mali Illangasekare, Vasant Jadhav, Michal Legiewicz, Irene Majerfeld, Jeremy Widmann and Michael Yarus, p. 783-806 -- Chapter 48. Aptamer Selection against Biological Macromolecules: Proteins and Carbohydrates / C. Stefan Vörtler and Maria Milovnikova, p. 807-839 -- Chapter 49. In Vivo SELEX Strategies / Thomas A. Cooper, p. 840-852 -- Chapter 50. In Vitro Selection against Small Targets / Dirk Eulberg, Christian Maasch, Werner G. Purschke and Sven Klussmann, p. 853-877 -- Chapter 51. SELEX Strategies to Identify Antisense and Protein Target Sites in RNA or Heterogeneous Nuclear Ribonucleoprotein Complexes / Martin Lützelberger, Martin R. Jakobsen and Jørgen Kjems, p. 878-894 -- Chapter 52. Gene Silencing Methods for Mammalian Cells: Application of Synthetic Short Interfering RNAs / Matthias John, Anke Geick, Philipp Hadwiger, Hans-Peter Vomlocher and Olaf Heidenreich, p. 896-909 -- Appendix. UV Spectroscopy for the Quantitation of RNA, p. 910-913.
  • 2011 Springer
    Hughes, T. R.
    Introduction to "a handbook of transcription factors" / T.R. Hughes -- An overview of prokaryotic transcription factors : a summary of function and occurrence in bacterial genomes / A.S. Seshasayee, K. Sivaraman and N.M. Luscombe -- A catalogue of eukaryotic transcription factor types, their evolutionary origin, and species distribution / M.T. Weirauch and T.R. Hughes -- Function and evolution of C2H2 zinc finger arrays / L. Stubbs, Y. Sun and D. Caetano-Anolles -- Homeodomain subtypes and functional diversity / T.R. Burglin -- Nuclear receptors : Small molecule sensors that coordinate growth, metabolism and reproduction / K. Pardee, A.S. Necakov and H. Krause -- Methods for analysis of transcription factor DNA-binding specificity in vitro / A. Jolma and J. Taipale -- Identification of transcription factor-DNA interactions in vivo / D.T. Odom -- How transcription factors identify regulatory sites in genomic sequence / Y. Field, E. Sharon and E. Segal -- Transcription factor binding sites and other features in human and Drosophila proximal promoters / C. Vinson, R. Chatterjee and P. Fitzgerald -- Interactions of transcription factors with chromatin / H. van Bakel -- Transcription factor effector domains / S. Frietze and P.J. Farnham -- Large-scale nuclear architecture and transcriptional control / J.M. Vaquerizas, A. Akhtar and N.M. Luscombe.
  • 2015 (30th ed.). AccessMedicine
    29th ed., 2012 AccessMedicine
    Bender, David A.; Botham, Kathleen M.; Granner, Daryl K.; Harper, Harold A.; Kennelly, Peter J.; Mayes, Peter A.; Murray, Robert K.; Rodwell, Victor W.; Weil, P. Anthony.
    Also available: Print – 2003-<2015>
  • 2014 ScienceDirect
    Komoda, Tsugikazu.
    The HDL Handbook: Biological Functions to Clinical Implications brings laboratory research in HDL from bench to bedside in this needed resource for researchers and clinicians studying cholesterol, lipids, epidemiology, biochemistry, molecular medicine, and pathophysiology of cardiovascular diseases. In addition, researchers and clinicians working with an aging population, corporate researchers, post-doctorates; medical students and graduate students will find this publication useful because the scope of coverage includes basic science, genetics, epidemiology, and treatment of HDL cholesterol as well as potential targets to modify HDL cholesterol. Provides bench-to-bedside coverage of HDL with thorough coverage of basic science, genetics, epidemiology, and treatment Presents a complete update with six new chapters on the latest advances in HDL cholesterol research with international perspective New chapters on proteomics, clinical impact of LCAT in HDL metabolism, and an in-depth discussion of potential targets to modify HDL provide a translational reference for clinicians.
  • 2013 Springer Protocols
    Vivanco, Fernando.
    Large-scale characterization of the murine cardiac proteome / Jake Cosme, Andrew Emili, and Anthony O. Gramolini -- Determining protein concentrations of the human ventricular proteome / Arjen Scholten and Albert J.R. Heck -- Multidimensional protein identification technology for direct-tissue proteomics of heart / Dario Di Silvestre, Francesca Brambilla, and Pier Luigi Mauri -- Global protein quantification of mouse heart tissue based on the SILAC Mouse / Anne Konzer [and others] -- Global proteomic profiling and enrichment maps of dilated cardiomyopathy / Ruth Isserlin, Daniele Merico, and Andrew Emili -- Characterization of the human myocardial proteome in dilated cardiomyopathy by label-free quantitative shotgun proteomics of heart biopsies / Elke Hammer, Katrin Darm, and Uwe Völker -- Systems proteomics of healthy and diseased chromatin / Haodong Chen [and others] -- Proteomic analysis of interstitial aortic valve cells acquiring a pro-calcific profile / Millioni Renato [and others] -- Differential protein expression analysis of degenerative aortic stenosis by iTRAQ labeling / Sergio Alonso-Orgaz [and others] -- Characterization of zebrafish cardiac proteome using online pH Gradient SCX-RP HPLC-MS/MS Platform / Jiang Zhang [and others] -- Proteomic analysis of brain mitochondrial proteome and mitochondrial complexes / Ana Lopez-Campistrous and Carlos Fernandez-Patron -- Oxidative modifications of mitochondria complex II / Liwen Zhang [and others] -- Detection of O-GlcNAc modifications on cardiac myofilament proteins / Genaro A. Ramirez-Correa [and others] -- Quantification of mitochondrial S-nitrosylation by CysTMT 6 Switch Assay / Christopher I. Murray [and others] -- Identification of thioredoxin target protein networks in cardiac tissues of a transgenic Mouse / Cexiong Fu [and others] -- Using pure protein to build a multiple reaction monitoring mass spectrometry assay for targeted detection and quantitation / Eric Grote [and others] -- Sequential extraction methodology for cardiac extracellular matrix prior to proteomics analysis / Javier Barallobre-Barreiro [and others] -- Optimized method for identification of the proteomes secreted by cardiac cells / Miroslava Stastna and Jennifer E. Van Eyk -- Secretome of human aortic valves / Fernando de la Cuesta [and others] -- Comparative study of immunodepletion and equalization methods for aortic stenosis human plasma / Felix Gil-Dones [and others].
  • 2007 Springer
    Asea, Alexzander A. A.; De Maio, Antonio.
  • 2008 Springer
    Asea, Alexzander A. A.; Brown, Ian R.
  • 2010 Springer
    Asea, Alexzander A. A.; Pedersen, Bente Klarlund.
  • 2007 Springer
    Ciocca, Daniel R.; Calderwood, Stuart K.; Sherman, Michael Y.
  • 2014 Springer
    Blatch, Gregory L.; Shonhai, Addmore.
    The Importance of Molecular Chaperones in Survival and Pathogenesis of the Malaria Parasite Plasmodium falciparum / Jude Przyborski -- General Structural and Functional Features of Molecular Chaperones / Adrienne L. Edkins and Aileen Boshoff -- The Role of Hsp70s in the Development and Pathogenicity of Plasmodium Species / Addmore Shonhai -- Role of the Hsp40 Family of Proteins in the Survival and Pathogenesis of the Malaria Parasite / Eva-Rachele Pesce, Alexander G. Maier and Gregory L. Blatch -- Role of Hsp90 in Plasmodium falciparum Malaria / Dea Shahinas and Dylan R. Pillai -- The Role of Parasite Heat Shock Proteins in Protein Trafficking and Host Cell Remodeling / Paul R. Gilson, Sarah C. Charnaud and Brendan S. Crabb -- Role of Heat Shock Proteins in Immune Modulation in Malaria / Evelyn Böttger and Gabriele Multhoff -- Establishment of Plasmodium falciparum Extracellular Compartments in its Host Erythrocyte / Alassane Mbengue, Laurence Berry and Catherine Braun-Breton -- Chaperones and Proteases of Plasmodium falciparum / Kaiyin Liu and Walid A. Houry -- Heat Shock Proteins as Targets for Novel Anti-Malarial Drugs / Manish Grover, Shweta Chaubey and Utpal Tatu -- Heat Shock Proteins of Malaria: What Do We Not Know, and What Should the Future Focus Be? / Addmore Shonhai and Gregory L. Blatch.
  • 2011 Springer
    Xie, Jingwu.
  • 2015 Springer Protocols
    Riobo, Natalia A.
    Luciferase reporter assays to study transcriptional activity of hedgehog signaling in normal and cancer cells / Silvia Pandolfi and Barbara Stecca -- Measuring expression levels of endogenous gli genes by immunoblotting and real-time PCR / Pawel Niewiadomski and Rajat Rohatgi -- Quantitative immunoblotting of endogenous hedgehog pathway components / Shohreh F. Farzan and David J. Robbins -- Measuring Gli2 phosphorylation by selected reaction monitoring mass spectrometry / Robert Ahrends, Pawel Niewiadomski, Mary N. Teruel, and Rajat Rohatgi -- Rapid screening of Gli2/3 mutants using the Flp-In system / Pawel Niewiadomski and Rajat Rohatgi -- Insights into gli factors ubiquitylation methods / Paola Infante, Romina Alfonsi, and Lucia Di Marcotullio -- Determination of acetylation of the gli transcription factors / Sonia Coni, Laura Di Magno, and Gianluca Canettieri -- Efficient detection of indian hedgehog during endochondral ossification by whole-mount immunofluorescence / João Francisco Botelho, Daniel Smith-Paredes, and Veronica Palma A. -- Methods for detection of Ptc1-driven LacZ expression in adult mouse skin / Donna M. Brennan-Crispi, My G. Mahoney, and Natalia A. Riobo -- Determination and analysis of cellular metabolic changes by noncanonical hedgehog signaling / Raffaele Teperino and John Andrew Pospisilik.
  • 2007 Springer Protocols
    Horabin, Jamila I.
    Purifying the hedgehog protein and its variants / Darren P. Baker, Frederick R. Taylor, and R. Blake Pepinsky -- Application of sonic hedgehog to the developing chick limb / Eva Tieck and Cheryll Tickle -- Manipulation of hedgehog signaling in Xenopus by means of embryo microinjection and application of chemical inhibitors / Thomas Hollemann .. [et al.] -- Isolation of rat telencephalic neural explants to assay Shh GABAergic interneuron differentiation-inducing activity / Rina Mady and Jhumku D. Kohtz -- Genetic analysis of the vertebrate hedgehog-signaling pathway using muscle cell fate specification in the zebrafish embryo / Sudipto Roy -- Efficient manipulation of hedgehog/GLI signaling using retroviral expression systems / Maria Kasper ... [et al.] -- Cell surface marker and cell cycle analysis, hedgehog signaling, and flow cytometry / Krsting Detmer and Ronald E. Garner -- Detecting tagged hedgehog with intracellular and extracellular immunocytochemistry for functional analysis / Ainhoa Callejo, Luis Quijada, and Isabel Guerrero -- Confocal analysis of hedgehog morphogenetic gradient coupled with fluorescent in situ hybridization of hedgehog target genes / Armel Gallet and Pascal P. Thérond -- RNA in the hedgehog signaling pathway : pFRiPE, a vector for temporally and spatially controlled RNAi in Drosophila / Eric Marois and Suzanne Eaton -- Germline clone analysis for maternally acting Drosophila hedgehog components / Erica M. Selva and Beth E. Stronach -- Clone analysis of hedgehog signaling in Drosophila somatic tissues / Christing M. Bankers and Joan E. Hooper -- GAL4/UAS targeted gene expression for studying Drosophila hedgehog signaling / Denise Bussona nd Anne-Marie Pret -- Biochemical fractionation of Drosophila cells / Melanie Stegman and David Robbins -- Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway / Chao Ton and Jin Jiang -- Sequence analyses to study the evolutionary history and cis-regulatory elements of hedgehog genes / Ferenc Müller and Anne-Gaelle Borycki.
  • 2006 Springer
    Ruiz i Altaba, Ariel.
    How the hedgehog outfoxed the crab: interference with HEDGEHOG-GLI signaling as anti-cancer therapy? / Ariel Ruiz i Altaba -- The patched receptor: switching on/off the hedgehog signaling pathway / Luis Quijada ... [et al.] -- Making a morphogenetic gradient / Henk Roelink -- Spatial and temporal regulation of hair follicle progenitors by hedgehog signaling / Anthony E. Oro -- Mode of PTCH1/Ptch1-associated tumor formation: insights from mutant Ptch1 mice / Heidi Hahn -- Basal cell carcinomas, hedgehog signaling, and the Ptch1+/- mouse / Ervin Epstein, Jr. -- GLI genes and their targets in epidermal development and disease / Fritz Aberger and Anna-Maria Frischauf -- Splitting hairs: dissecting the roles of Gli activator and repressor functions during epidermal development and disease / Pleasantine Mill and Chi-Chung Hui -- Shh expression in pulmonary injury and disease / Paul M. Fitch ... [et al.] -- Human correlates of GL13 function / Leslie G. Biersecker -- From oligodactyly to polydactyly: role of Shh and Gli3 in limb morphogenesis / Chin Chiang -- The genetics of Indian hedgehog / M. Elizabeth McCready and Dennis E. Bulman -- Sonic hedgehog signaling in craniofacial development / Dwight Cordero, Minal Tapadia and Jill A. Helms -- Important role of Shh controlling Gli3 functions during the dorsal-ventral patterning of the telencephalon / Jun Motoyama and Kazushi Aoto -- Regulation of early events in cell cycle progression by hedgehog signaling in CNS development and tumorigenesis / Anna Marie Kenney and David H. Rowitch -- Modulating the hedgehog pathway in diseases / Frederic J. de Sauvage and Lee L. Rubin -- Hedgehog signaling in endodermally derived tumors / Marina Pasca di Magliano and Matthias Hebrok.
  • 2012 Springer
    Lever, Rebecca; Mulloy, Barbara; Page, C. P.
    History of heparin / T.W. Barrowcliffe -- Heparin biosynthesis / Pernilla Carlsson and Lena Kjellén -- Anticoagulant and antithrombotic mechanisms of heparin / Elaine Gray, John Hogwood, and Barbara Mulloy -- Standardisation of unfractionated and low-molecular-weight heparin / Elaine Gray -- Structure and physicochemical characterisation of heparin / Barbara Mulloy -- Case study: contamination of heparin with oversulfated chondroitin sulfate / Edward K. Chess ... [et al.] -- Low-molecular-weight heparins: differential characterization/physical characterization / Marco Guerrini and Antonella Bisio -- Heparin and heparan sulfate: analyzing structure and microheterogeneity / Zachary Shriver ... [et al.] -- Heparin in the prophylaxis and treatment of venous thromboembolism and other thrombotic diseases / Paolo Gresele, Chiara Busti, and Gloria Paganelli -- Adverse effects of heparin / S. Alban -- Neutralization of heparin activity / Menaka Pai and Mark A. Crowther -- Non-anticoagulant effects of heparin: an overview / Rebecca Lever and Clive P. Page -- Glycosaminoglycan and chemokine/growth factor interactions / Janis Shute -- Glycosaminoglycans and neuroprotection / B. Dudas and K. Semeniken -- Heparan sulphate: a heparin in miniature / J.T. Gallagher -- Heparin mimetics / Deirdre R. Coombe and Warren C. Kett -- Hyaluronic acid / Luigi Allegra, Sabrina Della Patrona, and Giuseppe Petrigni -- Semi-synthetic heparinoids / P. Oreste and G. Zoppetti -- Heparin-like entities from marine organisms / S. Colliec-Jouault, C. Bavington, and C. Belbarre-Ladrat.
  • 2015 Springer
    Akasaka, Kazuyuki; Matsuki, Hitoshi.
    Part I. Why and how proteins denature under prssure? -- Part II. Volume, compressibility, fluctuation and interaction in proteins -- Part III. Pressure and functional sub-states in proteins -- Part IV. Pressure and protein folding and assembly -- Part V. Pressure effects on biological membranes -- Part VI. Pressure adaptation and tolerance of proteins and living organisms -- Part VII. High pressure food processing and sterilization -- Part VIII. Pressure effects on motility, physiology and health -- Part IX. Methodology.
  • 2009 Springer Protocols
    Doyle, Sharon A.
    Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh -- PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood -- High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina -- High-throughput insect cell protein expression applications / Mirjam Buchs ... [et al.] -- High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee -- The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens -- High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi -- Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio ... [et al.] -- Expression and purification of soluble His₆-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh -- High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery. High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt -- Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley -- Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath -- Flexi vector cloning / Paul G. Blommel ... [et al.] -- The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens -- The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley -- A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... [et al.] -- "System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart -- Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni²⁺-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery -- E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... [et al.].
  • 2010
    Wilcox, R. Bruce.
    Acid-base relationships -- Amino acids and proteins -- Enzymes -- Citric acid cycle and oxidative phosphorylation -- Carbohydrate metabolism -- Lipid metabolism -- Amino acid metabolism -- Nucleotide metabolism -- Nutrition -- Gene expression -- Biochemical technology -- Hormones.
  • 2003 ScienceDirect
    Dutta, Rinku; Inouye, Masayori.
  • 2013 CRCnetBASE
    Exbrayat, J. M.
    Section 1. Histological and histochemical methods with light microscopy -- section 2. Cytological methods in electron microscopy -- section 3. Image quantification.
  • 2006 Springer
    Verdin, Eric.
    Histone deacetylase 1 / Dominique Meunier and Christian Seiser -- Biochemistry of multiprotein HDAC complexes / Alejandro Vaquero, Michael Scher, and Danny Reinberg -- The biology of HDAC3 / Edward Seto -- The biology of HDAC8, a unique class I histone deacetylase / David Waltregny and Vincent Castronovo -- Regulation of muscle gene expression by histone deacetylases / Timothy A. McKinsey and Eric N. Olson -- The class IIA histone deacetylases: functions and regulation / Herbert G. Kasler and Eric Verdin -- Histone deacetylases in the response to misfolded proteins / J. Andrew McKee and Tso-Pang Yao -- Evolution of sirtuins from archaea to vertebrates / Roy A. Frye -- Structure of the Sir2 family of NAD+-dependent histone/protein deacetylases / Kehao Zhao and Ronen Marmorstein -- The enzymology of Sir2 proteins / Margie T. Borra and John M. Denu -- The class III protein deacetylases: homologs of yeast Sir2P / Bjoern Schwer ... [et al.] -- HDAC inhibitors: discovery, development, and clinical impacts / Akihiro Ito, Norikazu Nishino, and Mitsuaki Yoshida -- Cell cycle targets of histone deacetylase inhibitors / Brian Gabrielli -- HDAC inhibitors: an emerging anticancer therapeutic strategy / Paul Kwon, Meier Hsu, Dalia Cohen, and Peter Atadja.
  • 2005 Springer
    Belfort, Marlene.
  • 2013 Springer
    Jakubowski, Hieronim.
    Excess of homocysteine, a product of the metabolism of the essential amino acid methionine, is associated with poor health, is linked to heart and brain diseases in general human populations, and accelerates mortality in heart disease patients. Neurological and cardiovascular abnormalities occur in patients with severe genetic hyperhomocysteinemia and lead to premature death due to vascular complications. Although it is considered a non-protein amino acid, studies over the past dozen years have discovered mechanisms by which homocysteine becomes a component of proteins. Homocysteine-containing proteins lose their normal biological function and become auto-immunogenic and pro-thrombotic. In this book, the author, a pioneer and a leading contributor to the field, describes up-to date studies of the biological chemistry of homocysteine-containing proteins, as well as pathological consequences and clinical implications of their formation. This is a comprehensive account of the broad range of basic science and medical implications of homocysteine-containing proteins for health and disease.
  • 2012 Springer Protocols
    Abagyan, Ruben; Orry, Andrew J. W.
    Classification of proteins : available structural space for molecular modeling / Antonina Andreeva -- Effective techniques for protein structure mining / Stefan J. Suhrer [and others] -- Methods for sequence-structure alignment / Ceslovas Venclovas -- Force fields for homology modeling / Andrew J. Bordner -- Automated protein structure modeling with SWISS-MODEL workspace and the protein model portal / Lorenza Bordoli and Torsten Schwede -- Practical introduction to molecular dynamics simulations : applications to homology modeling / Alessandra Nurisso, Antoine Daina, and Ross C. Walker -- Methods for accurate homology modeling by global optimization / Keehyoung Joo, Jinwoo Lee, and Jooyoung Lee -- Ligand-guided receptor optimization / Vsevolod Katritch, Manuel Rueda, and Ruben Abagyan -- Loop simulations / Maxim Totrov -- Methods of protein structure comparison / Irina Kufareva and Ruben Abagyan -- Homology modeling of class A G protein-coupled receptors / Stefano Costanzi -- Homology modeling of transporter proteins (carriers and ion channels) / Aina Westrheim Ravna and Ingebrigt Sylte -- Methods for the homology modeling of antibody variable regions / Aroop Sircar -- Investigating protein variants using structural calculation techniques / Jonas Carlsson and Bengt Persson -- Macromolecular assembly structures by comparative modeling and electron microscopy / Keren Lasker [and others] -- Preparation and refinement of model protein-ligand complexes / Andrew J.W. Orry and Ruben Abagyan -- Modeling peptide-protein interactions / Nir London, Barak Raveh, and Ora Schueler-Furman -- Comparison of common homology modeling algorithms : application of user-defined alignments / Michael A. Dolan, James W. Noah, and Darrell Hurt.
  • 2010 Springer
    Calabrese, Edward J.; Mattson, Mark Paul.
  • 2008 ClinicalKey
    Litwack, Gerald.
    This textbook is specifically designed for upper-division undergraduate or graduate students in life science or pre-medical majors including dentistry or pharmacology, who are required to take a biochemistry or medical biochemistry course, but who are not necessarily biochemistry majors. The book adopts a unique approach to the topic compared with other biochemistry textbooks currently available, in that each biochemical subject is introduced by a human disease relating the biochemical principles to be developed in that chapter. The goal is to make biochemistry more meaningful to the student who is not normally shown the connection between biochemistry and medicine. This book: includes an abundance of figures; emphasizes human biochemistry; and introduces each chapter with a relevant disease or clinical relationship.
  • 2005 CRCnetBASE
    Coughtrie, Michael W. H.; Pacifici, Gian Maria.
    Nomenclature and molecular biology of the human sulfotransferase family / Rebecca Blanchard -- Structure and function of sulfotranserases / Jyrki Taskinen and W.H. Coughtrie -- PAPS and sulfoconjucation / Nancy B. Schwartz -- Sulfate conjugation: pharmacogenetics and pharmacogenomics / by Weinshilboum & Adjei -- Molecular cloning of the human cytosolic sulfotransferases / by C.N.Falany ... [et al.] -- Sulfotransferases in the human fetus and neonate / by Stanley, Coughtri and Hume -- Sulfation of thyroid hormones / by Monique H.A. Kester, Michael W.H. Coughtrie and Theo J. Vissor -- Estrogen sulfotransferase in breast cancer / by Pasqualini and Chetrite -- Sulfation of drugs / by Pacifici -- Human sult1a sulfotransferases / by Nadine Hempel ... [et al.] -- Hydroxysteroid sulfotransferases sult2a1, sult2b1a and sult2b1b / by Charles A. Strott -- Species differences in cytosol sulfotoransgerases / Kiyoshi Nagata, Miki Shimada, and Yasushi Yamazoe -- Activiation and inactivation of carconogens and mutagens by human sulfotranserases / by Hansruedi Glatt.
  • 2015 ScienceDirect
    pt. B ScienceDirect
    Cadenas, Enrique; Packer, Lester.
  • 2008 Springer
    Tachezy, Jan.
    Anaerobic eukaryotes in pursuit of phylogenetic normality: the evolution of hydrogenosomes and mitosomes / W. Martin -- Protein import into hydrogenosomes and mitosomes / S. D. Dyall, P. Doležal -- Structure of the hydrogenosome / M. Benchimol -- Hydrogenosomes of anaerobic ciliates / J. H. P. Hackstein ... [et al.] -- Metabolism of trichomonad hydrogenosomes / I. Hrdý, J. Tachezy, M. Müller -- Hydrogenosomes of anaerobic chytrids: an alternative way to adapt to anaerobic environments / J. H. P. Hackstein ... [et al.] -- The proteome of T. vaginalis hydrogenosomes / K. Henze -- Hydrogenosome: the site of 5-nitroimidazole activation and resistance / J. Kulda, I. Hrdý -- Mitosomes in parasitic protists / J. Tachezy, O. Šmíd -- The mitochondrion-related organelle of Cryptosporidium parvum / J. S. Keithly -- Mitochondrial remnant in blastocystis / N. Yarlett, K. S. W. Tan -- Possible mitochondria-related organelles in poorly-studied "amitochondriate" eukaryotes / V. Hampl, A. G. B. Simpson.
  • 2011 Springer
    Sorrentino, Matthew J.
    Cardiovascular Risk Assessment -- The Metabolic Syndrome -- The Role of Imaging in Cardiovascular Risk Assessment -- Inherited Lipoprotein Disorders -- Advanced Lipid Testing -- Therapeutic Lifestyle Change for the Prevention and Treatment of Hyperlipidemia and Coronary Artery Disease -- Drug Therapy for Dyslipidemia -- Hypertriglyceridemia -- Isolated Low HDL -- Lipoprotein(a) -- HIV with Dyslipidemia -- Treatment of Dyslipidemia in Patients with Renal Insufficiency and End-Stage Renal Disease.
  • 2013
    Senechal, Marjorie.
    "In the vein of A Beautiful Mind, The Man Who Loved Only Numbers, and Rosalind Franklin: The Dark Lady of DNA, this volume tells the poignant story of the brilliant, colorful, controversial mathematician named Dorothy Wrinch. Drawing on her own personal and professional relationship with Wrinch and archives in the United States, Canada, and England, Marjorie Senechal explores the life and work of this provocative, scintillating mind. Senechal portrays a woman who was learned, restless, imperious, exacting, critical, witty, and kind. A young disciple of Bertrand Russell while at Cambridge, the first women to receive a doctor of science degree from Oxford University, Wrinch's contributions to mathematical physics, philosophy, probability theory, genetics, protein structure, and crystallography were anything but inconsequential. But Wrinch, a complicated and ultimately tragic figure, is remembered today for her much publicized feud with Linus Pauling over the molecular architecture of proteins. Pauling ultimately won that bitter battle. Yet, Senechal reminds us, some of the giants of mid-century science--including Niels Bohr, Irving Langmuir, D'Arcy Thompson, Harold Urey, and David Harker--took Wrinch's side in the feud. What accounts for her vast if now-forgotten influence? What did these renowned thinkers, in such different fields, hope her model might explain? Senechal presents a sympathetic portrait of the life and work of a luminous but tragically flawed character. At the same time, she illuminates the subtler prejudices Wrinch faced as a feisty woman, profound culture clashes between scientific disciplines, ever-changing notions of symmetry and pattern in science, and the puzzling roles of beauty and truth" -- Provided by publisher. "A biography of Dorothy Wrinch" -- Provided by publisher.
  • 2014 Springer
    Gullberg, Donald E.
    "The integrin family is composed of 24 members and approximately ten years ago (2003) we published a book devoted to the nine I domain integrin subunits. In this second edition, I am pleased that most of the original authors have been able to contribute to the updated version. I domain containing integrins include collagen receptors and leukocyte receptors. In 2003 the knockout mouse phenotypes for all of the I domain integrins had not yet been published; they are now, and are summarized and discussed in this edition. Interestingly, a recent 10 integrin mutation in dogs has indicated that collagen-binding integrins in the musculoskeletal system might have much more severe phenotypes in larger animals/humans compared to the mild integrin phenotypes observed in collagen-binding integrin deficient mice. This finding is further discussed in the book. In the cancer field, the microenvironment is taking center stage, and here collagen receptors on fibroblasts are predicted to play important roles in paracrine signaling, in regulating tissue stiffness and matrix remodeling. New technologies, new mouse models in combination with analyses of I integrins in larger animals/humans are thus predicted to increase our knowledge about this group of receptors. With this in mind we look forward to another 10 years of research with I domain integrins."--Publisher's wevsite.
    Also available: Print – 2014
  • 2011
    Firestone, Ari Joseph; Chen, James K.; Meyer, Tobias; Nelson, W. J.; Scott, Matthew P.
    Proper activation of Hh signaling is a critical mediator of metazoan development whereas aberrant activation, in post-embryonic tissues, is associated with initiation or progression of numerous common tumors. In chapter 1 of this dissertation I review the history, development, and status of extant small-molecule Hh inhibitors. I discuss limitations in the current approaches utilized in the discovery of these compounds, and the successes and challenges associated with tackling this pathway with targeted therapeutics. In chapter 2, I detail our progress using high-throughput screening to discover new small-molecule inhibitors of Hh signaling by specifically targeting elements of the pathway downstream of the transmembrane protein Smoothened (Smo), the target of the vast majority of existing inhibitors. These efforts resulted in the identification and preliminary characterization of four small molecules, each of which displays a distinct and novel combination of effects on various downstream signaling processes including formation of the primary cilia, the Hh-dependent regulation of Gli proteolytic-processing, and activation of Gli transcription factors. These compounds therefore represent valuable probes for further dissection of mechanisms of Hh signaling downstream of Smo and possible leads in the development of mechanistically novel Hh targeted chemotherapeutics. The third chapter continues with an in depth study of the Hh antagonist capable of preventing formation of primary cilia. We name this compound and its structurally and functionally related congeners ciliobrevins. We observe that acute treatment of cells with ciliobrevins leads to an accumulation of proteins in the distal tips of primary cilia, a phenotype that is consistent with inhibition of dynein-dependent retrograde protein trafficking. Ciliobrevin treatment also causes defects in mitotic spindle formation and the subcellular movement of organelles that are reminiscent of those observed under dynein loss of function conditions. Finally we show that ciliobrevins inhibit dynein-dependent microtubule gliding and dynein ATPase activities in vitro, demonstrating that this motor protein represents the critical physiological target of these small-molecule Hh inhibtors. This dissertation is concluded by a broader review of the use and development of small-molecules to control additional developmental signaling pathways and processes such as differentiation and self-renewal. Alterations in fate choice are central to a plethora of human diseases and chemically manipulating these will be a critical aspect in the advancement of medical technology from the treatment of tumors, to the advent of regenerative therapies.
  • 2012 ScienceDirect
    Conn, P. Michael.
    Section 1. Viruses, microbes, and parasites. Microbial cells analysis by atomic force microscopy / David Alsteens ; Understanding parasite transmission through imaging approaches / Mirko Singer and Freddy Frischknecht ; Multiphoton microscopy applied for real-time intravital imaging of bacterial infections in vivo / Ferdinand X. Choong ... [et al.] ; Analysis of virus entry and cellular membrane dynamics by single particle tracking / Helge Ewers and Mario Schelhaas ; Imaging of live malaria blood stage parasites / Christof Grüring and Tobias Spielmann ; Escherichia coli K1 invasion of human brain microvascular endothelial cells / Lip Nam Loh and Theresa H. Ward -- Section 2. Disease states. Real-time imaging after cerebral ischemia : model systems for visualization of inflammation and neuronal repair / Pierre Cordeau and Jasna Kriz ; In vivo real-time visualization of leukocytes and intracellular hydrogen peroxide levels during a zebrafish acute inflammation assay / Luke Pase, Cameron J. Nowell, and Graham J. Lieschke ; Imaging protein oligomerization in neurodegeneration using bimolecular fluorescence complementation / Federico Herrera, Susana Gonçalves, and Tiago Fleming Outeiro ; 3D/4D functional imaging of tumor-associated proteolysis : impact of microenvironment / Kamiar Moin ... [et al.] -- Section 3. Other techniques. Live cell imaging in live animals with fluorescent proteins / Robert M. Hoffman ; Protein activation dynamics in cells and tumor micro arrays assessed by time resolved Förster resonance energy transfer / Véronique Calleja, Pierre Leboucher, Banafshe ́Larijani ; Imaging stem cell differentiation for cell-based tissue repair / Zhenghong Lee ... [et al.] ; Understanding the initiation of B cell signaling through live cell imaging / Angel M. Davey ... [et al.] ; A quantitative method for measuring phototoxicity of a live cell imaging microscope / Jean-Yves Tinevez ... [et al.] ; High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines / Davide Danovi ... [et al.] ; High-throughput screening in primary neurons / Punita Sharma ... [et al.] ; Live imaging fluorescent proteins in early mouse embryos / Panagiotis Xenopoulos, Sonja Nowotschin, and Anna-Katerina Hadjantonakis ; Methods for three-dimensional analysis of dendritic spine dynamics / Enni Bertling ... [et al.] ; Imaging cell competition in Drosophila imaginal discs / Shizue Ohsawa ... [et al.] ; Live cell imaging of the oviduct / Sabine Kölle -- Computational quantification of fluorescent leukocyte numbers in zebrafish embryos / Felix Ellett and Graham J. Lieschke -- Four-dimensional tracking of lymphocyte migration and interactions in lymph nodes by two-photon microscopy / Masahiro Kitano and Takaharu Okada.
  • 2012 ScienceDirect
    Conn, P. Michael.
    Monitoring cytoskeletal dynamics in living neurons using fluorescence photoactivation / Anne Gauthier-Kemper ... [et al.] -- Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays / Roy Edward -- Methods to measure actin treadmilling rate in dendritic spines / Mikko Koskinen, Enni Bertling, and Pirta Hotulainen -- Live-cell imaging of clathrin coats / Comert Kural and Tom Kirchhausen -- Imaging the mitotic spindle / Paul S. Maddox ... [et al.] -- Modeling of spatial intracellular signaling events in neurons / Wendy C. Wenderski and Susana R. Neves -- Imaging chromosome dynamics in meiosis in plants / Moira J. Sheehan and Wojciech P. Pawlowski -- Analyzing lysosomes in live cells / Paul R. Pryor -- Detection and quantification of biomolecular association in living cells using single-molecule microscopy / Mario Brameshuber and Gerhard J. Schütz -- Visualizing cardiac ion channel trafficking pathways / James W. Smyth and Robin M. Shaw -- Live cell imaging of the cytoskeleton / Eve G. Stringham ... [et al.] -- Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms / Naoki Watanabe -- Analysis of cell dispersion and migration by video-microscopy / Bonnomet Arnaud ... [et al.] -- Real time qualitative and quantitative GLUT4 translocation assay / Maleppillil Vavachan Vijayakumar and Manoj Kumar Bhat -- Advanced imaging of cellular signaling events / Marek Cebecauer, Jana Humpolíčková, and Jerémie Rossy -- Dynamic imaging of homo-FRET in live cells by fluorescence anisotropy microscopy / Subhasri Ghosh ... [et al.] -- Time-resolved luminescence resonance energy transfer imaging of protein-protein interactions in living cells / Harsha E. Rajapakse and Lawrence W. Miller -- Imaging of corticosteroid receptors in live cells / Mayumi Nishi -- Investigating second messenger signaling in vivo / Rüdiger Rudolf, Mathias Hafner, and Marco Mongillo -- Probes for intracellular RNA imaging in live cells / Philip J. Santangelo ... [et al.] -- Imaging the glycome in living systems / Boyangzi Li, Feiyan Mock, and Peng Wu -- Intracellular magnesium detection by fluorescent Indicators / Valentina Trapani ... [et al.] -- Illuminating mobile zinc with fluorescence : from cuvettes to live cells and tissues / Zhen Huang and Stephen J. Lippard -- Quantitative fluorescent live cell Imaging of intracellular Ca2+ and H+ ions in malaria parasites / Petra Rohrbach.
  • 2012 ScienceDirect
    Conn, P. Michael.
    Laser-induced radiation microbeam technology and simultaneous real-time fluorescence imaging in live cells / Stanley W. Botchway ... [et al.] -- A cell biologist's guide to high resolution imaging / Graeme Ball ... [et al.] -- Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo / André E.P. Bastos ... [et al.] -- Detecting and tracking nonfluorescent nanoparticle probes in live cells / Gufeng Wang and Ning Fang -- Fluorescence lifetime microscopy of tumor cell invasion, drug delivery, and cytotoxicity / Gert-Jan Bakker ... [et al.] -- Measuring membrane protein dynamics in neurons using fluorescence recovery after photobleach / Inmaculada M. González-González ... [et al.] -- Fluorescent speckle microscopy in cultured cells / Marin Barisic, António J. Pereira, and Helder Maiato -- Green-to-red photoconvertible mEosFP-aided live imaging in plants / Jaideep Mathur ... [et al.] -- Methods for cell and particle tracking / Erik Meijering, Oleh Dzyubachyk, and Ihor Smal -- Correlative light-electron microscopy : a potent tool for the imaging of rare or unique cellular and tissue events and structures / Alexander A. Mironov and Galina V. Beznoussenko -- Optical techniques for imaging membrane domains in live cells (live-cell palm of protein clustering) / Dylan M. Owen ... [et al.] -- Single live cell topography and activity imaging with the Shear-Force-Based Constant-Distance Scanning Electrochemical Microscope / Albert Schulte, Michaela Nebel, and Wolfgang Schuhmann -- Visualization of TGN-endosome trafficking in mammalian and drosophila cells / Satoshi Kametaka and Satoshi Waguri -- Live cell imaging with chemical specificity using dual frequency CARS microscopy / Iestyn Pope ... [et al.] -- Imaging intracellular protein dynamics by Spinning Disk Confocal microscopy / Samantha Stehbens ... [et al.] -- Visualizing dynamic activities of signaling enzymes using genetically encodable FRET-based biosensors : from designs to applications / Xin Zhou, Katie J. Herbst-Robinson, and Jin Zhang -- Live-cell imaging of Aquaporin-4 supramolecular assembly and diffusion / A.S. Verkman, Andrea Rossi, and Jonathan M. Crane -- Coiled-coil tag-probe labeling methods for live-cell imaging of membrane receptors / Yoshiaki Yano ... [et al.] -- Monitoring protein interactions in living cells with fluorescence lifetime imaging microscopy / Yuansheng Sun ... [et al.] -- Open source tools for fluorescent imaging / Nicholas A. Hamilton -- Nanoparticle PEBBLE sensors in live cells / Yong-Eun Koo Lee and Raoul Kopelman.
  • 2010 Springer
    Burry, Richard W.
  • 2010 Springer
    Buchwalow, Igor B.; Böcker, W.
  • 2011
    Meyer, Carissa Bove; Pfeffer, Suzanne R.; Straight, Aaron; Theriot, Julie A.
    Eukaryotic chromosomes segregate by attaching to microtubules of the mitotic spindle through a chromosomal microtubule-binding site called the kinetochore. Kinetochores assemble on a specialized chromosomal locus termed the centromere, characterized by the replacement of histone H3 in centromeric nucleosomes with the essential histone H3 variant centromere protein A (CENP-A). CENP-A nucleosomes are believed to epigenetically specify centromere identity, thus understanding how CENP-A chromatin is assembled and maintained is central to understanding chromosome segregation mechanisms. CENP-A nucleosome assembly requires the Mis18 complex and the CENP-A chaperone HJURP. HJURP binds to pre-nucleosomal CENP-A and facilitates the deposition of new CENP-A nucleosomes into centromeric chromatin. The mechanistic roles of the Mis18 complex in CENP-A assembly and maintenance are not well understood. The Mis18 complex and HJURP localize to centromeres in telophase/G1, when new CENP-A chromatin is assembled. The molecular interactions that control their targeting are unknown. Several constitutive centromere proteins that remain associated with CENP-A chromatin throughout the cell cycle have also been implicated in CENP-A assembly, including CENP-C. Their functions in CENP-A assembly are unknown. The first chapter of this thesis work describes the development of an in vitro system for centromeric chromatin assembly in Xenopus laevis egg extracts. We show that CENP-A assembly in extract recapitulates the cell cycle dependence and HJURP requirement of CENP-A assembly in somatic cells. We then use this in vitro system to identify a novel role for CENP-C in recruiting CENP-A assembly factors to the centromere to promote CENP-A assembly, described in Chapter 2. We show that CENP-C is required to target the Mis18 complex protein M18BP1 to Xenopus sperm centromeres in metaphase. In the absence of CENP-C, M18BP1 and HJURP targeting to centromeres is disrupted and new CENP-A assembly into centromeric chromatin is inhibited. We find that CENP-C interacts directly with M18BP1 through conserved domains in the CENP-C protein. Thus, CENP-C provides a link between existing CENP-A chromatin and the proteins required for new CENP-A nucleosome assembly. Overall, this work extends our mechanistic understanding of how the pre-existing centromere directs the local assembly of new CENP-A nucleosomes to ensure stable propagation of the centromere. Furthermore, the ability to assemble centromeric chromatin in vitro will provide a valuable tool for dissecting the biochemical and cell cycle regulatory mechanisms that control new CENP-A assembly and ensure faithful centromere propagation.
  • 2007 Springer Protocols
    Grandi, Guido.
    Energy systems for ATP regeneration in cell-free protein synthesis reactions / Kara A. Calhoun and James R. Swartz -- Continuous-exchange protein-synthesizing systems / Vladimir A. Shirokov ... [et al.] -- Cell-free production of integral membrane proteins on a preparative scale / Christian Klammt ... [et al.] -- SIMPLEX : a novel method for high-throughput construction and screening of protein libraries / Suang Rungpragayphan, Tsuneo Yamane, and Hideo Nakano -- Methods for high-throughput materialization of genetic information based on the wheat germ cell-free expression system / Tatsuya Sawasaki ... [et al.] -- Exogenous protein expression in Xenopus oocytes : basic procedures / Elena Bossi ... [et al.] -- In vitro translation to study HIV protease activity / Zene Matsuda ... [et al.] -- The protein truncation test in mutation detection and molecular diagnosis / Oliver Hauss and Oliver Muller -- Creation of novel enantioselective lipases by SIMPLEX / Yuichi Koga, Tsuneo Yamane, and Hideo Nakano -- In vitro transcription and translation coupled with two-dimensional electrophoresis for bacterial proteome analysis / Nathalie Norais ... [et al.] -- In vitro screen of bioinformatically selected Bacillus anthracis vaccine candidates by coupled transcription, translation, and immunoprecipitation analysis / Orit Gat, Haim Grosfeld, and Avigdor Shafferman -- Functional expression of Type 1 rat GABA transporter in microinjected Xenopus laevis oocytes / Stefano Giovannardi ... [et al.] -- Production of protein for nuclear magnetic resonance study using the wheat germ cell-free system / Toshiyuki Kohno and Yaeta Endo.
  • 2003 ScienceDirect
    Aalbersberg, W. Y.
  • 2005 CRCnetBASE
    Srivastava, Sudhir.
    Chapter 1. The Promise of Proteomics: Biology, Applications, and Challenges / Paul D. Wagner and Sudhir Srivastava -- Chapter 2. Proteomics Technologies and Bioinformatics / Sudhir Srivastava and Mukesh Verma -- Chapter 3. Creating a National Virtual Knowledge Environment.
  • 2007 CRCnetBASE
    Smith, Paul J.; Yue, Eddy W.
  • 2005 Springer
    Alexander, Denis; Cohen, Patricia T. W.; Pinna, Lorenzo A.
  • 2009 Springer
    Şener, Bilge.
  • 2009 Springer Protocols
    Barker, Christopher J.
    The role of inositol and the principles of labelling, extraction, and analysis of inositides in mammalian cells / Christopher J. Barker and Per-Olof Berggren -- HPLC separation of inositol polyphosphates / Christopher J. Barker, Christopher Illies, and Per-Olof Berggren -- Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in mammalian cells / James C. Otto and John D. York -- A femtomole-sensitivity mass assay for inositol hexakisphosphate / Andrew J. Letcher, Michael J. Schell, and Robin F. Irvine -- Synthesis of InsP₇ by the inositol hexakisphosphate kinase 1 (IP6K1) / Cristina Azevedo ... [et al.] -- Protein pyrophosphorylation by diphosphoinositol pentakisphosphate (InsP₇) / J. Kent Werner Jr., Traci Speed, and Rashna Bhandari -- Synthesis and nonradioactive micro-analysis of diphosphoinositol phosphates by HPLC with postcolumn complexometry / Hongying Lin, Karsten Lindner, and Georg W. Mayr -- Diphosphosinositol polyphosphates and energy metabolism : assay for ATP/ADP ratio / Andreas Nagel ... [et al.] -- Isolation of inositol 1,4,5-triphosphate receptor-associating proteins and selective knockdown using RNA interference / Akihiro Mizutani ... [et al.] -- Phosphoinositide-specific phospholipase C [beta]1 signal transduction in the nucleus / Roberta Fiume ... [et al.] -- Methods to assess changes in the pattern of nuclear phosphoinositides / Nullin Divecha -- Measurement of phosphoinositide 3-kinase products in cultured mammalian cells by HPLC / Frank T. Cooke -- Phosphoinositide analysis by liquid chromatography-mass spectrometry / Trevor R. Pettitt -- Imaging phosphoinositide dynamics in living cells / Anne Wuttke, Olof Idevall-Hagren, and Anders Tengholm.
  • 2003. ScienceDirect
    Kuksis, Arnis.
    Also available: Print – 2003
  • 2010 Springer
    Thany, Steeve Herve.
    Identification of cholinergic synaptic transmission in the insect nervous system / Steeve Hervé Thany, Hélène Tricoire-Leignel, and Bruno Lapied -- The evolution of pentameric ligand-gated ion channels / Joseph A. Dent -- Diversity of insect nicotinic acetylcholine receptor subunits / Andrew K. Jones and David B. Sattelle -- Identification of critical elements determining toxins and insecticide affinity, ligand binding domains, and channel properties / Hélène Tricoire-Leignel and Steeve Hervé Thany -- Electrophysiological studies and pharmacological properties of insect native nicotinic acetylcholine receptors / Steeve Hervé Thany -- Characterisation of insect nicotinic acetylcholine receptors by heterologous expression / Neil S. Millar and Stuart J. Lansdell -- Neonicotinoid insecticides : historical evolution and resistance mechanisms / Steeve Hervé Thany -- Ecotoxicity of neonicotinoid insecticides to bees / Axel Decourtye and James Devillers -- State of the art on insect nicotinic acetylcholine receptor function in learning and memory / Monique Gauthier.
    Also available: Print – 2010
  • 2015 Springer Protocols
    García-Fruitós, Elena.
    General introduction : recombinant protein production and purification of insoluble proteins / Neus Ferrer-Miralles ... [et al.] -- Overcoming the solubility problem in E. coli : available approaches for recombinant protein production / Agustín Correa and Pablo Oppezzo -- Optimization of culture parameters and novel strategies to improve protein solubility / Ranjana Arya ... [et al.] -- Cleavable self-aggregating tags (cSAT) for protein expression and purification / Zhanglin Lin ... [et al.] -- Beyond the cytoplasm of Escherichia coli : localizing recombinant proteins where you want them / Jason T. Boock ... [et al.] -- Characterization of amyloid-like properties in bacterial intracellular aggregates / Anna Villar-Pique, Susanna Navarro, and Salvador Ventura -- Co-translational stabilization of insoluble proteins in cell- free expression systems / Lei Kai ... [et al.] -- Functional expression of plant membrane proteins in Lactococcus lactis / Sylvain Boutigny .... [et al.] -- High cell-density expression system : yeast cells in a phalanx efficiently produce a certain range of "Difficult-to-Express" secretory recombinant proteins / Yasuaki Kawarasaki, Takeshi Kurose, and Keisuke Ito -- Insect cells-baculovirus system for the production of difficult to express proteins / Judit Osz-Papai ... [et al.] -- Transient expression in HEK 293 cells : an alternative to E. coli for the production of secreted and intracellular mammalian proteins / Joanne E. Nettleship ... [et al.] -- Recombinant glycoprotein production in human cell lines / Kamilla Swiech ... [et al.] -- Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 / Maria Giuliani ... [et al.] -- Screening methodology for purifying proteins with aggregation problems / Mario Lebendiker, Michal Maes, and Assaf Friedler -- Solubilization and refolding of inclusion body proteins / Anupam Singh, Vaibhav Upadhyay, and Amulya K. Panda -- Bacterial inclusion body purification / Joaquin Seras-Franzoso ... [et al.] -- Characterization of intracellular aggresomes by fluorescent microscopy / Lianwu Fu and Elizabeth Sztul -- Dialysis : a characterization method of aggregation tendency / Mireia Pesarrodona, Ugutz Unzueta, and Esther Vázquez -- Applications of mass spectrometry to the study of protein aggregation / Sílvia Bronsoms and Sebastián A. Trejo -- Insoluble protein assemblies characterized by Fourier transform infrared spectroscopy / Antonino Natalello and Silvia M. Doglia -- Insoluble protein characterization by circular dichroism (CD) spectroscopy and nuclear magnetic resonance (NMR) / Shaveta Goyal ... [et al.] -- Methods for characterization of protein aggregates / Witold Tatkiewicz ... [et al.] -- Predicting the solubility of recombinant proteins in Escherichia coli / Roger G. Harrison and Miguel J. Bagajewicz -- Insoluble protein applications : the use of bacterial inclusion bodies as biocatalysts / Eva Hrabárová , Lucia Achbergerová , and Jozef Nahálka.
  • 2006 ScienceDirect
    Romeo, John T.
    Terpene indole alkaloid biosynthesis / Sarah E. O'Connor, Elizabeth McCoy -- Pathways to diverse ergot alkaloid profiles in fungi / Daniel G. Panaccione, Christopher L. Schardl, Christine M. Coyle -- Compartmentalization of plant secondary metabolism / Nailish Samanani, Peter J. Facchini -- Comparative genomics of the shikimate pathways in Arabidopsis, Populus trichocarpa and Oryza sativa : shikimate pathway gene family structure and identification of candidates for missing links in pehnylalanine biosynthesis / Björn Hamberger ... [et al.] -- Tomato glandular trichomes as a model system for exploring evolution of specialized metabolism in a single cell / Eyal Fridman ... [et al.] -- Anacardic acid biosynthesis and bioactivity / David J. Schultz, Nalinie S. Wickramasinghe, Carolyn M. Klinge -- Molecular and biochemical investigations of sorgoleone biosynthesis / Daniel Cook ... [et al.] -- Biosynthesis of terpenophenolic metabolites in hop and cannabis / Jonathan E. Page, Jana Nagel -- Engineering pathway enzymes to understand the function and evolution of sterol structure and activity / Pruthvi Jayasimha ... [et al.] -- Methylation and demethylation of plant signaling molecules / Yue Yang ... [et al.] -- Recent advances in auxin biosynthesis and conjugation / Amber Kei Bowers, Yunde Zhao -- Auxin biology and biosynthesis / Jessica Calio, Yuen Yee Tam, Jennifer Normanly -- Translational opportunities in plant biochemistry / Cecilia A. McIntosh.
  • 2007 ScienceDirect
    Cheresh, David A.
    Also available: Print – 2007
  • 2016 ScienceDirect
    Sonnenberg, Arnoud; Wilson, Katherine L.
    BioID identification of lamin-associated proteins -- Proximity-dependent biotin identification (BioID) in Dictyostelium Amoebae -- Purification and structural analysis of LEM-domain proteins -- Purification and structural analysis of SUN and KASH domain proteins - Purification and lamins and soluble fragments of NETs -- Simple separation of functionally distinct populations of lamin-binding proteins -- Development of a novel green fluroescent protein-based binding assay to study the association of plakins with intermediate filament proteins -- Functional analysis of keratin-associated proteins in intestinal epithelia: heat-shock protein chaperoning and kinase rescue -- Purification of protein chaperones and their functional assays with intermediate filaments -- Purification and structural analysis of plectin and BPAG1e -- Purification and structural analysis of desmoplakin -- Degradation of the intermediate filament family by gigaxonin -- Functional and genetic analysis of plectin in skin and muscle -- Functional and genetic analysis of epiplakin in epithelial cells -- In vitro model of the epidermis: connecting protein function to 3D structure -- Functional analysis of periplakin and envoplakin, cytoskeletal linkers, and cornified envelope precursor proteins -- Microtubule-actin cross-linking factor 1: domains, interaction partners, and tissue-specific functions -- Functional and genetic analysis of neuronal isoforms of BPAG1 -- Functional and genetic analysis of spectraplakins in Drosophila -- Functional and genetic analysis of VAB-10 spectraplakin in Caenorhabditis elegans -- Tagged chromosomal insertion site system: a method to study lamina-associated chromatin -- Lamin-binding proteins in caenorhabditis elegans -- Pathologically relevant prelamin A interactions with transcription factors -- MCLIP detection of novel protein-protein interactions at the nuclear envelope -- Preparation of the human cytomegalovirus nuclear egress complex and associated proteins.
  • 2016 ScienceDirect
    Liem, Ronald K. H.; Omary, M. Bishr.
    Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in vertebrates and invertebrates, providing a unique understanding of the multiple different tissue-specific intermediate filaments. This volume also covers the latest methods that are currently being used to study intermediate filament protein function and dynamics. It will be an important companion for any experimentalist interesting in studying this protein family in their cell or organism model system.
  • 2006 Springer
    Paramio, Jesús M.
    Desmin and other intermediate filaments in normal and diseased muscle / Denise Paulin and Zhigang Xue -- Intermediate filaments in astrocytes in health and disease / Milos Pekny and Ulrika Wilhelmsson -- Neuronal intermediate filaments and neurodegenerative diseases / Gee Y. Ching and Ronald K.H. Liem -- Neurofilaments: phosphorylation and signal transduction / Sashi Kesavapany, Richard H. Quarles and Harish C. Pant -- Keratin intermediate filaments and diseases of the skin / E. Birgitte Lane -- The keratin K6 minifamily of genes / Manuel Navarro -- Transcriptional regulation of keratin gene expression / Miroslav Blumenberg -- Simple epithelial keratins: expression, function and disease / M. Llanos Casanova, Ana Bravo and José L. Jorcano -- Keratins as targets in and modulators of liver diseases / Kurt Zatloukal ... [et al.] -- The search for specific keratin functions: the case of keratin K10 / Mirentxu Santos ... [et al.].
  • 2007 Springer
    Hooper, Nigel M.; Lendeckel, Uwe.
    Site-2 protease at ten -- Signal peptide peptidases -- GXGD-type intramembrane proteases -- Rhomboid intramembrane serine proteases -- Proteases of the Rhomboid family in the yeast Saccharomyces cerevisiae -- [gamma]-Secretase and Alzheimer's disease -- [gamma]-Secretase mediated proteolysis: at the cutting edge of notch signaling.
  • 2015 Springer
    Felli, Isabella C.; Pierattelli, Roberta.
    1. Back to the future: nuclear magnetic resonance and bioinformatics studies on intrinsically disordered proteins -- 2. Structure and dynamics of intrinsically disordered proteins -- 3. NMR methods for the study of intrinsically disordered proteins structure, dynamics, and interactions: general overview and practical guidelines -- 4. Ensemble calculation for intrinsically disordered proteins using NMR parameters -- 5. NMR spectroscopic studies of the conformational ensembles of intrinsically disordered proteins -- 6. Recombinant intrinsically disordered proteins for NMR: tips and tricks -- 7. Biophysical methods to investigate intrinsically disordered proteins: avoiding an "elephant and blind men" situation -- 8. Application of SAXS for the structural characterization of IDPs -- 9. Bioinformatics approaches for predicting disordered protein motifs -- 10. Towards understanding protein disorder in-cell -- 11. The Protein Ensemble Database -- 12. Order and disorder in the replicative complex of paramyxoviruses -- 13. Druggability of intrinsically disordered proteins -- 14. Beta amyloid hallmarks: from intrinsically disordered proteins to Alzheimer's Disease.
    Also available: Print – 2015
  • 2007 Springer
    Leach, Andrew R.; Gillet, Valerie J.
  • 2002 Wiley
    Tsai, C. Stan.
    ch. 1. Introduction, p. 1-10 -- ch. 2. Biochemical Data: Analysis and Management, p. 11-40 -- ch. 3. Biochemical Exploration: Internet Resources, p. 41-52 -- ch. 4. Molecular Graphics: Visualization of Biomolecules, p. 53-71 -- ch. 5. Biochemical Compounds: Structure and Analysis, p. 73-105 -- ch. 6. Dynamic Biochemistry: Biomolecular Interactions, p. 107-121 -- ch. 7. Dynamic Biochemistry: Enzyme Kinetics, p. 123-146 -- ch. 8. Dynamic Biochemistry: Metabolic Simulation, p. 147-163 -- ch. 9. Genomics: Nucleotide Sequences and Recombinant DNA, p. 165-182 -- ch. 10. Genomics: Gene Identification, p. 183-208 -- ch. 11. Proteomics: Protein Sequence Analysis, p. 209-231 -- ch. 12. Proteomics: Prediction of Protein Structures, p. 233-268 -- ch. 13. Phylogenetic Analysis, p. 269-284 -- ch. 14. Molecular Modeling: Molecular Mechanics, p. 285-314 -- ch. 15. Molecular Modeling: Protein Modeling, p. 315-341 -- Appendices.
  • 2010 Wiley
    Mishra, Nawin C.
    "Proteomics provides an introductory insight on proteomics, discussing the basic principles of the field, how to apply specific technologies and instrumentation, and example applications in human health and diseases. With helpful study questions, this textbook presents an easy to grasp and solid overview and understanding of the principles, guidelines, and especially the complex instrumentation operations in proteomics for new students and research scientists. Written by a leader in proteomics studies, Proteomics offers an expert perspective on the field and the future of proteomics"--Provided by publisher.
    Also available: Print – v. [52], 2010.
  • 2003 CRCnetBASE
    Hatti-Kaul, Rajni; Mattiasson, Bo.
  • 2012 Springer
    Atreya, Hanudatta S.
    Also available: Print – 2012
  • 2016 ScienceDirect
    Kelman, Zvi.
    Application of natural isotopic abundance 1H-13C-and 1H-15N-correlated two-dimensional NMR for evaluation of the structure of protein therapeutics -- A semiautomated assignment protocol for methyl group side chains in large proteins -- 19F-site-specific-labeled nucleotides for nucleic acid structural analysis by NMR -- Applying thymine isostere 2,4-difluoro-5-methylbenzene as a NMR assignment tool and probe of homopyrimidine/homopurine tract structural dynamics -- Biomolecular deuteration for neutron structural biology and dynamics -- Dueterium labeling together with contrast variation small-angle neutron scattering suggests how skip captures and releases unfolded outer membrane proteins -- Deuteration in biological neutron reflectometry -- Deuterium labeling strategies for creating contrast in structure-function studies of model bacterial outer membranes using neutron reflectometry -- Essential strategies for revealing nanoscale protein dynamics by neutron spin echo spectoscopy -- Method for the determination of 15N incorporation percentage in labeled peptides and proteins -- QconCAT: internal standard for protein quantification -- Production purification, and characterization of 15N-labeled DNA repair proteins as internal standards for mass spectrometric measurements -- Hydrogen exchange mass spectrometry -- Mapping protein-ligand interactions with proteolytic fragmentation, hydrogen/deuterium exchange-mass spectrometry -- Isotope labelgin of biomolecules: structural analysis of viruses by HDX-MS.
  • 2015 ScienceDirect
    Kelman, Zvi.
    Isotope Labeling of Biomolecules - Labeling Methods, the latest volume of the Methods in Enzymology series contains comprehensive information on stable isotope labeling methods and applications for biomolecules.
  • 2012 Springer
    Decker, Thomas; Müller, Mathias.
    The Continuing Fascination with Jaks and Stats: An Introduction / Thomas Decker and Mathias Müller -- Regulation of JAKs: Insights Gleaned from the Functional Protein Domains / Claude Haan, Daniela Ungureanu, Tuija Pekkala, Olli Silvennoinen and Serge Haan -- Nuclear Functions of the Janus Kinases / Mark A. Dawson and Andrew J. Bannister -- Self-association of STAT Proteins from Monomers to Paracrystals / Mathias Droescher and Uwe Vinkemeier -- How Stats Interact with the Molecular Machinery of Transcriptional Activation / Amanda M. Jamieson, Matthias Farlik and Thomas Decker -- Nongenomic Functions of STAT3 / Daniel J. Gough, Pravin Sehgal and David E. Levy -- Evolution of Jak and Stat Proteins / Claus Vogl, Priyank Shukla and Ingo Ebersberger -- JAK/STAT and Chromatin Regulation in Drosophila / Kriti Gaur and Willis X. Li -- JAK/STAT Signaling and Invertebrate Immune Responses / Feng Zhou and Hervé Agaisse -- Stat5 as a Hematopoietic Master Regulator for Differentiation and Neoplasia Development / Harini Nivarthi, Katrin Friedbichler and Richard Moriggl -- Regulation of Dendritic Cell Development by STATs / Haiyan S. Li and Stephanie S. Watowich. STAT Transcription Factors: Controlling All Aspects of NK Cell Biology / Eva Maria Putz, Eva Zebedin and Veronika Sexl -- Function of JAKs and STATs in Lymphocytes: Bench to Bedside / Alejandro Villarino, Xiang-Ping Yang, Kiyoshi Hirahara, Yuka Kanno and John J. O' Shea, et al. -- The Role of Janus Kinases in Hematopoietic Malignancies / Damla Olcaydu and Robert Kralovics -- Activation of Janus Kinases During Tumorigenesis / Jeffrey W. Schmidt and Kay-Uwe Wagner -- STAT1 and STAT3 Transcription Factors in Inflammation-associated Colon Cancer / Paulina Rampetsreiter and Robert Eferl -- Universal and Specific Functions of STAT3 in Solid Tumours / Lidia Avalle, Gabriella Regis and Valeria Poli -- Impact of STAT5 on Normal Tissue Development and Cancer / Maria M. Caffarel and Christine J. Watson -- Crosstalk with the Jak-STAT Pathway in Inflammation / Lionel B. Ivashkiv -- Activation and Inhibition of JAK-STAT Signal Transduction by RNA Viruses / Curt M. Horvath -- Multifaceted Antiviral Actions of Interferon-stimulated Gene Products / Birgit Strobl, Nicole R. Leitner and Mathias Müller -- Interferon Regulation of the Innate Response to Bacteria / Ali Abdul-Sater and Christian Schindler.
  • 2011 Springer
    Rodríguez-Moreno, Antonio; Sihra, Talvinder S.
    "This volume critically examines the functional actions of the kainate type glutamate receptors (KARs). Following on from the larger body of work on the NMDA and AMPA-type ionotropic glutamate receptors (GluRs), studies with KARs have consistently thrown up exceptions to general rules about synaptic modulation. Contributors herein provide an insight to the idiosyncracies that now almost typify the KAR field. The fascinating insights provided in this volume serve to encourage searching mechanistic questions"--Provided by publisher.
    Also available: Print – 2011
  • 2016 Springer Protocols
    Goueli, Said A.; Zegzouti, Hicham.
    HTRF kinase assay development and methods in inhibitor characterization / Yong Jia, Mari Manuia, and Jose Juarez -- Application of eukaryotic elongation factor-2 kinase (eEF-2K) for cancer therapy : expression, purification, and high-throughput inhibitor screening / Clint D. J. Tavares ... [et al.] -- Recombinant kinase production and fragment screening by NMR spectroscopy / Byeonggu Han and Hee-Chul Ahn -- Bioluminescence methods for assaying kinases in quantitative high-throughput screening (qHTS) format applied to yes1 tyrosine kinase, glucokinase, and PI5P4Kα lipid kinase / Mindy I. Davis, Douglas S. Auld, and James Inglese -- Using bioluminescent kinase profiling strips to identify kinase inhibitor selectivity and promiscuity / Hicham Zegzouti, Jacquelyn Hennek, and Said A. Goueli -- Measuring activity of phosphoinositide lipid kinases using a bioluminescent ADP-detecting assay / Andrew W. Tai and Jolanta Vidugiriene -- High-throughput radiometric kinase assay / Krisna C. Duong-Ly and Jeffrey R. Peterson -- High-content assay to screen for modulators of EGFR function / Christophe Antczak and Hakim Djaballah -- Monitoring protein kinase expression and phosphorylation in cell lysates with antibody microarrays / Hong Zhang, Xiaoqing Shi, and Steven Pelech -- From enzyme to whole blood : sequential screening procedure for identification and evaluation of p38 MAPK inhibitors / Silke M. Bauer ... [e tal.] -- Genetically encoded fluorescent indicators to visualize protein phosphorylation in living cells / Moritoshi Sato and Yoshio Umezawa -- Characterization of an engineered Src kinase to study Src signaling and biology / Leanna R. Gentry .. [et al.] -- Screening one-bead-one-compound peptide libraries for optimal kinase substrates / Thi B. Trinh and Dehua Pei -- Determination of the substrate specificity of protein kinases with peptide micro- and macroarrays / Shenshen Lai ... [et al.] -- Rapid identification of protein kinase phosphorylation site motifs using combinatorial peptide libraries / Chad J. Miller and Benjamin E. Turk.
  • 2005 CRCnetBASE
    Jurisica, Igor; Wigle, Dennis.
  • 2013 ScienceDirect
    Lorsch, Jon.
    Section I. Cell protocols : basic microbiological techniques. Pouring agar plates and streaking or spreading to isolate individual colonies / Elizabeth Eyler ; Storage of bacteria and yeast / Cheryl M. Koh -- Section II. Cell protocols : cellular fractionation. Cellular fractionation : mammalian cells / Joerg von Hagen and Uwe Michelsen ; Cellular fractionation : yeast cells / Joerg von Hagen and Uwe Michelsen -- Section III. Cell protocols : Knock-outs and gene replacements. Yeast-gene replacement using PCR products / Megan Bergkessel, Christine Guthrie, and John Abelson ; Measuring RNAi knockdown using qPCR / Stuart Milstein, Michael Nguyen, Rachel Meyers, and Antonin de Fougerolles ; Recombineering : using drug cassettes to knock out genes in vivo / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court ; Gene knockouts, in vivo site-directed mutagenesis and other modifications using the delitto perfetto system in Saccharomyces cerevisiae / Samantha Stuckey and Francesca Storici ; Gene targeting and site-specific recombination in mouse ES cells / Konstantinos Anastassiadis, Frank Schnütgen, Harald von Melchner, and A. Francis Stewart ; Recombineering : highly efficient in vivo genetic engineering using single-strand oligos / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court -- Section IV. Cell protocols : microbiological media. Growth media for E. coli / Juliane C. Lessard ; Saccharomyces cerevisiae growth media / Jessica S. Dymond --Section V. Cell protocols : preparation of slides for microscopy. Immunohistochemistry on freely floating fixed tissue sections / Julia Bachman ; Preparation of slides for microscopy from frozen tissue sections / Julia Bachman ; Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry / Kirstie Canene-Adams ; Preparation of cells for microscopy using cytospin / Cheryl M. Koh ; Preparation of cells for microscopy using chamber slides and coverslips / Cheryl M. Koh ; Preparation of cells for microscopy using 'cell blocks' / Cheryl M. Koh -- Section VI. Lipid and carbohydrate protocols. Enzymatic deglycosylation of glycoproteins / Min-Sung Kim and Dan Leahy -- Section VII. Lipid and carbohydrate protocol : making vesicles and micelles. Preparation of fatty acid or phospholipid vesicles by thin-film rehydration / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Preparation of fatty acid micelles / Ting F. Zhu, Itay Budin, and Jack W. Szostak -- Section VIII. Other protocols. Reverse-phase HPLC analysis and purification of small molecules / Kirstie Canene-Adams ; Thin layer chromatography / Marina Santiago and Scott Strobel ; ATP and GTP hydrolysis assays (TLC) / Vaishnavi Rajagopal and Jon R. Lorsch.
  • pt. A-D, 2014-15. ScienceDirect
    pt. B ScienceDirect
    pt. C ScienceDirect
    pt. D ScienceDirect
    Lorsch, Jon.
  • 2013 ScienceDirect
    Lorsch, Jon.
    Computational prediction of RNA secondary structure -- Reverse-transcription PCR (RT-PCR) -- Northern blotting -- Explanatory chapter: nuclease protection assays -- In vitro transcription from plasmid or PCR-amplified DNA -- Measurement of in vivo synthesis rates -- Method for measuring mRNA decay rate in saccharomyces cerevisiae -- Analysis of polysomes from bacteria -- Polysome profile analysis - yeast -- Polysome analysis of mammalian cells -- Polysome analysis for determining mRNA and ribosome association in saccharomyces cerevisiae -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates -- Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3' ends, and poly(A) tails of RNA -- RNA radiolabeling -- Fluorescently labeling synthetic RNAs -- Analysis of RNA by analytical polyacrylamide gel -- RNA purification by preparative polyacrylamide gel -- Explanatory chapter: nucleic acid concentration determination -- RNA purification-precipitation methods -- Reverse transcriptase dideoxy sequencing of RNA -- RNA structure experimental analysis-chemical modification -- Structural analysis of RNA backbone using in-line probing.
  • 2012 Springer
    Takayama, Yoshiharu.
    "Lactoferrin was first identified as an iron-binding glycoprotein abundantly found in milk. It has been considered to play important roles in host defense, since lactoferrin shows broad spectrum of anti-bacterial and anti-viral activities, arising from its iron-chelating property. Furthermore, lactoferrin can regulate the function of innate and adaptive immune cells and exhibits immuno-modulating properties. These activities of lactoferrin implicate that potential use of lactoferrin as a wound healing agent. In addition, this book shows that lactoferrin is a potent regulator of dermal fibroblasts and epidermal keratinocytes, and promotes skin or corneal epithelial wound healing by increasing their proliferation, migration or deposition of extracellular matrix (ECM) components such as collagen and hyaluronan. This book provides the scientific background on use of lactoferrin as a wound healing agent. Indeed, lactoferrin exerts its biological effects by binding to specific lactoferrin receptors on target cells. This book also covers the in-depth information on roles of lactoferrin as a signaling molecule."--Publisher's website.
  • 2014 Springer Protocols
    Hirabayashi, Jun.
  • 2012 Springer Protocols
    Sandoval, Georgina.
    Lipases : an overview / Leticia Casas-Godoy [and others] -- Lipases or esterases : Does it really matter? Toward a new bio-physico-chemical classification/ Yassine Ben Ali, Robert Verger, and Abdelkarim Abousalham -- Study of new feruloyl esterases to understand lipase evolution : the case of Bacillus flexus / Mónica Sánchez-González [and others] -- Phospholipases : an overview / Ahmed Aloulou [and others] -- High-throughput screening method for lipases/esterases / Eduardo Mateos-Díaz [and others] -- Functional-based screening methods for lipases, esterases, and phospholipases in metagenomic libraries/ Dolores Reyes Duarte, Manuel Ferrer, and Humberto García-Arellano -- Plant lipases : partial purification of Carica papaya lipase / Ivanna Rivera, Juan Carlos Mateos-Díaz, and Georgina Sandoval -- Plant phospholipases : an overview / Geliang Wang, Stephen Ryu, and Xuemin Wang -- Lipase and phospholipase inhibitors : design and applications / Mark E. Venable -- High-throughput assay of secreted phospholipases A₂ inhibitors / Wallace Thompson [and others] -- Heterologous expression systems for lipases : a review / Francisco Valero -- Streptomyces phospholipase D cloning and production / Yozo Nakazawa -- Cloning of PLD2 from baculovirus for studies in inflammatory responses / Julian Gomez-Cambronero and Karen M. Henkels -- Lipases production by solid-state fermentation : the case of Rhizopus homothallicus in perlite / Susana Velasco-Lozano, Tania Volke-Sepulveda, and Ernesto Favela-Torres -- Lipases and esterases from extremophiles : overview and case example of the production and purification of an esterase from Thermus thermophilus HB27 / Pablo Fuciños [and others] -- Purification, biochemical and kinetic properties of recombinant Staphylococcus aureus lipase / Habib Horchani [and others] -- Phospholipase A2 purification and characterization : a case study / Aida Carray [and others] -- The yeast Yarrowia lipolytica as a generic tool for molecular evolution of enzymes / Sophie Duquesne [and others] -- Molecular modeling of lipase binding to a substrate-water interface / Christian C. Gruber and Jürgen Pleiss -- Different strategies for hyperactivation of lipase biocatalysts / Jose M. Palomo and Jose M. Guisan -- Rational design of immobilized lipases and phospholipases / Alberto del Monte-Martínez and Bessy V. Cutiño-Avila -- Thermodynamical methods for the optimization of lipase-catalyzed reactions / Edmundo Castillo [and others] -- Lipases as biocatalysts for the synthesis of structured lipids / Ram Chandra [and others] -- Lipase-catalyzed modification of phenolic antioxidants / Pamela Torres [and others] -- Lipases as catalysts in synthesis of fine chemicals / Alicia Baldessari -- Lipases in green chemistry : acylation and alcoholysis on steroids and nucleosides / Alicia Baldessari and Luis E. Iglesias -- Lipases as biocatalyst for biodiesel production / Xiaohu Fan, Xochitl Niehus, and Georgina Sandoval -- Synthesis of biodegradable polymers using biocatalysis with Yarrowia lipolytica lipase / Karla A. Barrera-Rivera, Arturo Flores-Carreón, and Antonio Martínez-Richa -- Phospholipases in food industry : a review / Victor Casado [and others] -- Lipase and phospholipase biosensors : a review / Enrique J. Herrera-López.
  • 2009 Springer
    Jeannin, Jean-François.
    Structure and synthesis of lipid A / Shoichi Kusumoto, Masahito Hashimoto and Kazuyoshi Kawahara -- Conformation and supramolecular structure of lipid A / Klaus Brandenburg and Ulrich Seydel -- Interactions between lipid A and serum proteins / Jèorg Andrèa ... [et al.] -- The lipid A receptor / Kiyoshi Takeda -- Lipid A receptor TLR4-mediated signaling pathways / Masahiro Yamamoto and Shizuo Akira -- Lipid A-induced responses in vivo / Néjia Sassi ... [et al.] -- Lipid A-mediated tolerance and cancer therapy / Cheryl E. Rockwell, David C. Morrison and Nilofer Qureshi -- Lipid A in cancer therapies : preclinical results / Daniele Reisser and Jean-François Jeannin -- Monophosphoryl lipid A (MPL) as an adjuvant for anti-cancer vaccines : clinical results / Christopher W. Cluff -- Antitumoral effects of lipids A : clinical studies / Marc Bardou and Danièle Reisser.
    Also available: Print – 2009
  • 2006 CRCnetBASE
    Mossoba, Magdi M.
    An overview of modern mass spectrometry methods in the toolbox of lipid chemists and biochemists / R. Moreau -- Techniques and applications in lipid analysis / Nils Hinrichsen and Hans Steinhart -- Recent advances in silver-ion HPLC utilizing acetonitrile in hexane as solvent: temperature effects on lipid elution orders/resolution / R.O. Adlof -- TLC-FID with special reference to marine lipids and other high-molecular-weight organic compounds / R.G. Ackman and A. Timmins -- Analysis of trans-18 :1 fatty acids by silver ion HPLC / P. Delmonte and M.P. Yurawecz -- Lipid separations using packed-column SFC / D.G. Hayes -- LC-MS and chiral separation / A. Kuksis -- LC-MS and lipid oxidation / A. Kuksis -- Structural analysis of unsaturated fatty acid methyl ester isomers with acetonitrile covalent adduct chemical ionization (CACI) / J.T. Brenna -- Fast GC for cellular fame analysis of bacteria / J.S. Buyer -- Use of cellular fatty acids to identify food-borne pathogens by infrared spectroscopy and capillary GC / M.M. Mossoba -- Infrared spectroscopy and partial least square calibration in the simultaneous quantification of isolated trans and conjugated linoleic acids / Alfred A. Christy -- Global cellular lipidome analyses by shotgun lipidomics using multidimensional mass spectrometry / X. Han and R.W. Gross -- Waxes and sterols: structures and chemistry / E.J. Parish and A.D. Bell -- Analysis of biological tissue and edible fat / J.K.G. Kramer -- Investigation of protein-lipid interactions by vibrational spectroscopy / E.C.Y. Li-Chan, G. Meng and N.K. Howell -- Fat replacers: an overview / W.E. Artz -- High-performance size-exclusion chromatography for lipid analysis in organic media / M.C. Dobarganes and G. Marquez-Ruiz -- Phospholipids : structures and physicochemical activities / M.C. Erickson.
  • 2014 Springer
    Kato, Yoji.
    This volume focusses on oxidative modifications of lipid molecules and the successive generation of singlet oxygen. The book also covers the secondary adductions of these reactive species with proteins and aminophospholipids. During lipid peroxidation, the initial event is the formation of lipid hydroperoxide, which is followed by an oxidation event that starts a chain-reaction. The formed lipid hydroperoxide gradually decomposes into harmful aldehydes, which are the advanced end-products of lipid peroxidation.
  • 2015 Springer
    Yassine, Hussein.
    This timely, concise title provides an important update on clinical lipid management. Using information from recent clinical trials and in special populations, the book begins by offering an easy-to-read overview of LDL, HDL, and triglyceride metabolism and the genetics of lipid disorders. The link between inflammation and lipids, and how this relates to atherosclerosis development, is also addressed, as are the measures of subclinical atherosclerosis in patients with abnormal lipid levels. Lipid abnormalities in children, with a particular focus on vulnerable populations (with an emphasis on ethnicity and childhood obesity), are covered. The treatment goals and approaches for managing lipids in the clinic are thoroughly discussed, emphasizing the important role of statin use and addressing controversies of lipid management in special populations such as heart failure, end stage kidney disease and fatty liver disease. Of special note, an important update on how new HIV medications impact lipid levels is provided. In all, Lipid Management: From Basics to Clinic, is an invaluable, handy resource for understanding changes in lipids in different populations and for sharpening the clinical approach to managing complicated lipid cases.
  • 2011 Springer
    Farooqui, Akhlaq A.
    "Lipid Mediators and Their Metabolism in the Brain presents readers with cutting edge and comprehensive information not only on the synthesis and degradation of glycerophospholipid-, sphingolipid-, and cholesterol-derived lipid mediators, but also their involvement in neurological disorders. It is hoped that this monograph will be useful not only to postgraduate student and their teachers, but also to research scientists and physicians, who are curious about the generation and roles of lipid mediators in the brain."--Publisher's website.
  • 2007 Springer Protocols
    McIntosh, Thomas J.
    Overview of membrane rafts / Thomas J. McIntosh -- Analysis of raft affinity of membrane proteins by detergent-insolubility / Deborah A. Brown -- Nondetergent isolation of rafts / Mehul B. Shah and Pravin B. Sehgal -- Detecting ordered domain formation (lipid rafts) in model membranes using tempo / Omar Bakht and Erwin London -- Using monomolecular films to characterize lipid lateral interactions / Rhoderick E. Brown and Howard L. Brockman -- Electro-formation and fluorescence microscopy of giant vesicles with coexisting liquid phases / Sarah L. Veatch -- Fluorescence correlation spectroscopy / Kristen Bacia and Petra Schwille -- Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells / Avery L. McIntosh ... [et al.] -- NMR detection of lipid domains / Ivan V. Polozov and Klaus Gawrisch -- Lateral diffusion coefficients of raft lipids from pulsed field gradient NMR / Greger Orädd and Göran Lindblom -- Saturation-recovery electron paramagnetic resonance discrimination by oxygen transport (DOT) method for characterizing membrane domains / Witold K. Subczynski ... [et al.] -- Plasmon-waveguide resonance spectoscopy studies of lateral segregation in solid-supported proteolipid bilayers / Zdzislaw Salamon, Savitha Devanathan, and Gordon Tollin -- Fluorescence recovery after photobleaching studies of lipid rafts / Anne K. Kenworthy -- Single-molecule tracking / Marija Vrljic, Stefanie Y. Nishimura, and W.E. Moerner -- X-ray diffraction to determine the thickness of raft and nonraft bilayers / Thomas J. McIntosh -- Small-angle neutron scattering to detect rafts and lipid domains / Jeremy Pencer ... [et al.] -- Exploring membrane domains using native membrane sheets and transmission electron microscopy / Bridget S. Wilson ... [et al.] -- Atomic force microscopy of lipid domains in supported model membranes / Alan R. Burns -- Atomistic and coarse-grained computer simulations of raft-like lipid mixtures / Sagar A. Pandit and H. Larry Scott -- A microscopic model calculation of the phase diagram of ternary mixtures of cholesterol and saturated and unsaturated phospholipids / R. Elliott, I. Szleifer, and M. Schick.
  • 2009 Springer Protocols
    Larijani, Banafshé; Rosser, Colin A.; Woscholski, Rudiger.
    Eicosanoids : generation and detection in mammalian cells / Valerie B. O'Donnell, Ben Maskrey, and Graham W. Taylor -- Lipidomic analysis of phospholipids and related structures by liquid chromatography-mass spectrometry / Trevor R. Pettitt -- Measurement of polyphosphoinositides in cultured mammalian cells / Frank T. Cooke -- Inositol lipid-dependent functions in Saccharomyces cerevisiae : analysis of phosphatidylinositol phosphates / Stephen K. Dove and Robert H. Michell -- Methods for the determination of the masss of nuclear PtdIns4P, PtdIns5P, and PtdIns(4,5)P₂ / David R. Jones ... [et al.] -- Lipid quantification and structure determination of nuclear envelope precursor membranes in the sea urchin / Marie Garnier-Lhomme ... [et al.] -- Solution and solid-state NMR of lipids / Axelle Grelard ... [et al.] -- Spatial localization of PtdInsP₂ in phase-separated giant unilamellar vesicles with a fluorescent PLC-delta 1 PH domain / Xavier Mulet ... [et al.] -- Studying cell-to-cell interactions : an easy method of tethering ligands on artificial membranes / Sebastian J. Fleire and Facundo D. Batista -- Ceramide-induced transbilayer (flip-flop) lipid movement in membranes / F.-Xabier Contreras ... [et al.] -- Effects of sterols on the development and aging of Caenorhabditis elegans / Eun-Young Lee ... [et al.] -- Proteomic analysis of the sterol-mediated signaling pathway in Caenorhabditis elegans / Byung-Kwon Choi ... [et al.] -- Sudying the subcellular localization and DNA-binding activity of FoxO transcription factors, downstream effectors of P13K/Akt / Abdelkader Essafi ... [et al.] -- Measurement of PTEN activity in vivo by imaging phosphorylated Akt / Erika Rosivatz and Rudiger Woscholski -- Analysis of phosphatidylinositol 3,4,5 trisphosphate 5-phosphatase activity by in vitro and in vivo assays / Lisa M. Ooms ... [et al.] -- Measuring phospholipase D activity in insulin-secreting pancreatic [beta]-cells and insulin-responsive muscle cells and adipocytes / Rosanna Cazzolli ... [et al.] -- Protein kinase C as an effector of lipid-derived second messengers / Marie-Hélène Paclet ... [et al.] -- Detection of myotubularin phosphatases activity on phosphoinositides in vitro and ex vivo / Holger Maria Rohde ... [et al.] -- Quantification of multiple phosphatidylinositol 4-kinase isozyme activities in cell extracts / Mark G. Waugh, Shane Minogue, and J. Justin Hsuan -- Phospholipid-interacting proteins by solution-state NMR spectroscopy / Keiichiro Kami, Sundaresan Rajesh, and Michael Overduin -- Revealing signaling in single cells by single- and two-photon fluorescence lifetime imaging microscopy / Damien Alcor, Veronique Calleja, and Banafshé Larijani -- Phosphoinositide (PI) 3-kinase assays / Michael J. Fry -- Measurement of phosphatidylinositol and phosphatidylcholine binding and transfer activity of the lipid transport Protein PITP / Shamshad Cockcroft -- In vitro reconstitution of activation of PLC[epsilon] by Ras and Rho GTPases / Natalia Lamuño Gandarillas ... [et al.] -- Assaying endogenous phosphatidylinositol-4-phosphate 5-kinase (PIP5K) activities / Jonathon R. Halstead ... [et al.] -- Preparation of membrane rafts / Mark G. Waugh and J. Justin Hsuan.
  • 2016 Springer Protocols
    Waugh, Mark.
    Method for assaying the lipid kinase phosphatidylinositol-5-phosphate 4-kinase α in quantitative high-throughput screening (qHTS) bioluminescent format / Mindy I. Davis, Atsuo T. Sasaki, and Anton Simeonov -- Assaying ceramide synthase activity in vitro and in living cells using liquid chromatography-mass spectrometry / Xin Ying Lim, Russell Pickford, and Anthony S. Don -- Fluorescent assays for ceramide synthase activity / Timothy A. Couttas and Anthony S. Don -- Identification of the interactome of a palmitoylated membrane protein, phosphatidylinositol 4-kinase type II alpha / Avanti Gokhale ... [et al.] -- Measurement of long-chain fatty Acyl-CoA synthetase activity / Joachim Füllekrug and Margarete Poppelreuther -- Qualitative and quantitative in vitro analysis of phosphatidylinositol phosphatase substrate specificity / Laura Ren Huey Ip and Christina Anja Gewinner -- Luciferase reporter assays to assess liver X receptor transcriptional activity / Matthew C. Gage, Benoit Pourcet, and Inés Pineda-Torra -- Metabolically biotinylated reporters for electron microscopic imaging of cytoplasmic membrane microdomains / Kimberly J. Krager and John G. Koland -- Fluorescence recovery after photobleaching analysis of the diffusional mobility of plasma membrane proteins : HER3 mobility in breast cancer cell membranes / Mitul Sarkar and John G. Koland -- Isolation and analysis of detergent-resistant membrane fractions / Massimo Aureli ... [et al.] -- Detection of isolated mitochondria-associated ER membranes using the sigma-1 receptor / Abasha Lewis, Shang-Yi Tsai, and Tsung-Ping Su -- Using surface plasmon resonance to quantitatively assess lipid-protein interactions / Kathryn Del Vecchio and Robert V. Stahelin -- Analyzing protein-phosphoinositide interactions with liposome flotation assays / Ricarda A. Busse ... [et al.] -- High-throughput fluorometric assay for membrane-protein interaction / Wonhwa Cho, Hyunjin Kim, and Yusi Hu -- Guidelines for the use of protein domains in acidic phospholipid imaging / Matthieu Pierre Platre and Yvon Jaillais -- Analysis of sphingolipid synthesis and transport by metabolic labeling of cultured cells with [3H]serine / Neale D. Ridgway -- Determination and characterization of tetraspanin-associated phosphoinositide-4 kinases in primary and neoplastic liver cells / Krista Rombouts and Vinicio Carloni -- Analysis of the phosphoinositide composition of subcellular membrane fractions / Deborah A. Sarkes and Lucia E. Rameh -- Single-molecule imaging of signal transduction via GPI- anchored receptors / Kenichi G. N. Suzuki -- Measuring phosphatidylinositol generation on biological membranes / Mark Waugh -- Assay for CDP-diacylglycerol generation by CDS in membrane fractions / Mark Waugh.
  • 2013 Springer
    Capelluto, Daniel G. S.
    Sphingomyelin and its role in cellular signaling / Mahua Chakraborty and Xian-Cheng Jiang -- The driving force of alpha-synuclein insertion and amyloid channel formation in the plasma membrane of neural cells: key role of ganglioside- and cholesterol-binding domains / Jacques Fantini and Nouara Yahi -- The enigmatic role of sulfatides: new insights into cellular functions and mechanisms of protein recognition / Shuyan Xiao, Carla V. Finkielstein, and Daniel G. S. Capelluto -- Phosphoinositides and PDZ domain scaffolds / Anna Maria Wawrzyniak, Rudra Kashyap and Pascale Zimmermann -- PtdIns(4)P signalling and recognition systems / Marc Lenoir and Michael Overduin -- PtdIns(4,5)P2-mediated cell signaling: emerging principles and PTEN as a paradigm for regulatory mechanism / Arne Gericke, Nicholas R. Leslie, Mathias Lösche, and Alonzo H. Ross -- Role of phosphatidylinositol 3,4,5-trisphosphate in cell signaling / Robert D Riehle, Sinziana Cornea and Alexei Degterev -- The role of phosphoinositides and inositol phosphates in plant cell signaling / Glenda E. Gillaspy -- Phosphatidic acid-mediated signaling / Yu Liu, Yuan Su, and Xuemin Wang -- Phosphatidylserine-mediated cellular signaling / Jason G. Kay and Sergio Grinstein -- Cardiolipin-mediated cellular signaling / Vinay A. Patil and Miriam L. Greenberg.
    Also available: Print – 2013
  • 2010 CRCnetBASE
    Murphy, Eric J.; Rosenberger, Thad A.
    Methods for measuring the activity and expression of phospholipases A2 / by Prerna Rastogi, Janhavi Sharma, and Jane McHowat -- Methods for measuring the activity and expression of phospholipase C / by Ryan A. Frieler, John A. Watt, and Thad A. Rosenberger -- Methods for measuring the activity and expression of phospholipase D / by Wenjuan Su and Michael A. Frohman -- Methods for measuring the activity and expression of diacylglycerol kinase / by Kaoru Goto ... [et al.] -- Methods for measuring endocannabinoid production and expression and activity of enzymes involved in the endocannabinoid system / by Tiziana Bisogno, Luciano De Petrocellis, and Vincenzo Di Marzo -- Mass spectrometry methods for the analysis of lipid molecular species: a shotgun lipidomics approach / by Xianlin Han ... [et al.] -- Mass spectrometry methods for the analysis of bioactive sphingolipids : a high-performance liquid-chromatography/tandem mass spectrometry approach / by Jacek Bielawski, Motohiro Tani, and Yusuf A. Hannun -- Mass spectrometry methods for the analysis of oxidant stress in biological fluids and tissues : quantification of F2-isoprostanes and F4-neuroprostanes using mass spectrometry / by Ginger L. Mine and Jason D. Morrow -- Methods for assessing the role of lipid-activated nuclear receptors in regulation of gene transcription / by Jenny Kaeding, Jocelyn Trottier, and Olivier Barbier -- Fluorescence methods to assess the impact of lipid binding proteins on ligand-mediated activation of gene expression / by Heather A. Hostetler ... [et al.] -- Methods for measuring fatty acids using chromatographic techniques / by Gwendolyn Barecló-Coblijn, Cameron C. Murphy, and Eric J. Murphy -- Methods for measuring fatty acid uptake and targeting in cultured cells and in vivo / by Paul G. Millner, Serena M. Lackman, and Eric J. Murphy -- Methods for kinetic analysis of fatty acid incorporation and turnover in vivo : a steady-state kinetic radiotracer approach / by Dhaval P. Bhatt and Thad A. Rosenberger.
  • 2007 ScienceDirect
    Brown, H. Alex.
    Also available: Print – 2007
  • 2007 ScienceDirect
    Brown, H. Alex.
    Also available: Print – 2007
  • 2007 ScienceDirect
    Brown, H. Alex.
    Also available: Print – 2007
  • 2013 Springer Protocols
    Kleinschmidt, Jörg H.
    Quartz crystal microbalances as tools for probing protein-membrane interactions / Søren B. Nielsen and Daniel E. Otzen -- Surface plasmon resonance for measuring interactions of proteins with lipid membranes / Vesna Hodnik and Gregor Anderluh -- Probing the thermodynamics of protein-lipid interactions by isothermal titration calorimetry / Musti J. Swamy and Rajeshwer S. Sankhala -- Differential scanning calorimetry of protein-lipid interactions / Olga Canadas and Cristina Casals -- Atomic force microscopy and spectroscopy to probe single membrane proteins in lipid bilayers / K. Tanuj Sapra -- Optimized negative-staining protocol for examining lipid-protein interactions by electron microscopy / Mark Garewal, Lei Zhang, and Gang Ren -- Examining protein-lipid complexes using neutron scattering / Luke A. Clifton, Cameron Neylon, and Jeremy H. Lakey -- Circular-dichroism and synchrotron-radiation circular-dichroism spectroscopy as tools to monitor protein structure in a lipid environment / Koichi Matsuo and Kunihiko Gekko -- Structural characterization of membrane proteins and peptides by FTIR and ATR-FTIR spectroscopy / Suren A. Tatulian -- Forster resonance energy transfer as a tool for quantification of protein-lipid selectivity / Luıs M.S. Loura, Manuel Prieto, and Fabio Fernandes -- Guide to tracking single transmembrane proteins in supported lipid bilayers / Kumud Raj Poudel, Jeffrey P. Jones, and James A. Brozik -- Fluorescence correlation spectroscopy to examine protein-lipid interactions in membranes / Viktoria Betaneli and Petra Schwille -- Analyzing transmembrane protein and hydrophobic helix topography by dual fluorescence quenching / Gregory A. Caputo and Erwin London -- Studying lipid-protein interactions with electron paramagnetic resonance spectroscopy of spin-labeled lipids / Tibor Pa li and Zoltan Kota -- EPR techniques to probe insertion and conformation of spin-labeled proteins in lipid bilayers / Enrica Bordignon and Yevhen Polyhach -- Solid-state NMR approaches to study protein structure and protein-lipid interactions / Christopher Aisenbrey [and others] -- Solution NMR spectroscopy for the determination of structures of membrane proteins in a lipid environment / Ashish Arora -- Nanodiscs as a New Tool to Examine Lipid-Protein Interactions / Mary A. Schuler, Ilia G. Denisov, and Stephen G. Sligar -- Simulation approach to lipid-protein interactions / Teresa Paramo [and others].
  • 2008 Springer
    Quinn, Peter J.; Wang, Xiaoyuan.
    Offers an account of the properties of the classes of lipids that are located in the membranes of various cells. This title presents a balanced perspective of what is known about the function of these lipids in healthy cells and tissues and what consequences follow malfunctions in their synthesis and metabolism.
  • 2016 Springer
    Li-Beisson, Yonghua; Nakamura, Yuki.
    This book summarizes recent advances in understanding the functions of plant and algal lipids in photosynthesis, in development and signaling, and in industrial applications. As readers will discover, biochemistry, enzymology and analytical chemistry, as well as gene knock-out studies have all contributed to our rapidly increasing understanding of the functions of lipids. In the past few decades, distinct physical and biochemical properties of specific lipid classes were revealed in plant and algal lipids and the functional aspects of lipids in modulating critical biological processes have been uncovered. These chapters from international authors across relevant research fields highlight the underlying evolutionary context of lipid function in photosynthetic unicellular and multicellular organisms. The book goes on to encompass what lipids can do for industrial applications at a time of fascination with plants and algae in carbon fixation and as sources for production of food, energy and novel chemicals. The developmental context is a part of the fresh and engaging perspective that is presented in this work which graduate students and scientists will find both illuminating and useful.
  • 2015 Springer
    Gursky, Olga.
    Role of Lipids in Folding, Misfolding and Function of Integral Membrane Proteins -- Protein Misfolding in Lipid-Mimetic Environments.- Lipids in Amyloid-[beta] Processing, Aggregation, and Toxicity -- Role of Cholesterol and Phospholipids in Amylin Misfolding, Aggregation and Etiology of Islet Amyloidosis -- Stability, Oligomerization, and Amyloidogenicity of Apo Serum Amyloid A -- Interactions of Lipid Membranes with Fibrillar Protein Aggregates -- The Role of Lipid in Misfolding and Amyloid Fibril Formation by Apolipoprotein C-II -- Amyloid-Forming Properties of Human Apolipoproteins: Sequence Analyses and Structural Insights -- Computational Approaches to Identification of Aggregation Sites and the Mechanism of Amyloid Growth -- Role of Syndecans in Lipid Metabolism and Human Diseases
    Also available: Print – 2015
  • 2012 ScienceDirect
    Di Paolo, Gilbert; Wenk, Markus R.
    Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding / Min Wu, Pietro de Camilli -- Studying lipids involved in the endosomal pathway / Christin Bissig, Shem Johnson, Jean Gruenberg -- Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking / Jean-Baptiste Manneville, Cécile Leduc, Benoit Sorre, Guillaume Drin -- Reconstituting multivesicular body biogenesis with purified components / Thomas Wollert -- Approaches to the study of Atg8-mediated membrane dynamics in vitro / Anjai Jotwani, Diana N. Richerson, Isabelle Motta, Omar Julca-Zevallos, Thomas J. Melia -- Reconstitution assay system for ceramide transport with semi-intact cells / Keigo Kumagai, Masahiro Nishijima, Kentaro Hanada -- Visualizing mitochondrial lipids and fusion events in mammalian cells / Huiyan Huang, Michael A. Frohman -- Targeted and non-targeted analysis of membrane lipids using mass spectrometry / Xue Li Guan, Markus R. Wenk -- Modulation of host phosphoinositide metabolism during salmonella invasion by the type III secreted effector SopB / Bernhard Roppenser, Sergio Grinstein, John H. Brumell -- Acute manipulation of phosphoinositide levels in cells / Belle Chang-Ileto, Samual G. Frere, Gilbert Di Paolo -- Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins / Marnix Wieffer, Volker Haucke, Michael Krauß -- Phosphoinositides at the neuromuscular junction of drosophila melanogaster : a genetic approach / Jan R. Slabbaert, Thang Manh Khuong, Patrik Verstreken -- Devising powerful genetics, biochemical and structural tools in the functional analysis of phosphatidylinositol transfer proteins (PITPs) across diverse species / James M. Davison, Vytas A. Bankaitis, Ratna Ghosh -- Genome-wide screens for gene products regulating lipid droplet dynamics / Weihua Fei, Hongyuan Yang -- The three dimensionality of cell membranes : lamellar to cubic membrane transition as investigated by electron microsocopy / Ketpin Chong, Yuru Deng -- Quantitative imaging of lipid metabolism in yeast : from 4D analysis to high content screens of mutant libraries / Heimo Wolinski, Kristian Bredies, Sepp D. Kohlwein -- Analysis of cholesterol trafficking with fluorescent probes / Frederick R. Maxfield, Daniel Wüstner -- Fluorescence correlation methods for imaging cellular behavior of sphingolipid-interacting probes / Rachel Kraut, Nirmalya Bag, Thorsten Wohland -- Monitoring phospholipid dynamics during phagocytosis : application of genetically-encoded fluorescent probes / Helen Sarantis, Sergio Grinstein -- Genetically encoded probes for phosopatidic acid / Nawal Kassas, Petra Tryoen-Tóth, Matthias Corrotte, Tamou Thahouly, Marie-France Bader, Nancy J. Grant, Nicolas Vitale.
  • 2013 Springer Protocols
    Freeman, Lita A.
    The lipid-rich and otherwise challenging nature of many key tissues complicates many aspects of current research, and applications of the unique nature of lipoproteins and their biological effects has engendered unique and vital methodologies. In Lipoproteins and Cardiovascular Disease: Methods and Protocols, experts in the field present a compendium of advanced and classical molecular biology methods targeted towards lipoprotein, atherosclerosis, and vascular biology research, bringing together in a single volume an updated set of protocols and strategies for methods now driving the most recent advances, along with classical methods that are still widely used. Among the many topics covered in this cutting-edge work, the book delves into crucial techniques such as quantitative real-time PCR, microarrays, RT-PCR laser capture microdissection, and tissue-specific gene overexpression, knockout, and knockdown methodologies, including AAV as a liver-directed gene delivery vehicle. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Lipoproteins and Cardiovascular Disease: Methods and Protocols serves both novices and experts alike as a complete guide for any researcher with an interest in lipoproteins and their significant biological effects.
  • pt. A-G, 2003-09 ScienceDirect
    pt. B, 2003 ScienceDirect
    pt. C, 2003 ScienceDirect
    pt. D, 2004 ScienceDirect
    pt. E, 2005 ScienceDirect
    pt. F, 2009 ScienceDirect
    pt. G, 2009 ScienceDirect
    Düzgüneş, Nejat.
  • 2008
    Champe, Pamela C.; Ferrier, Denise R.; Harvey, Richard A.
    Status: Not Checked OutLane Catalog Record
    Unit I: Protein structure and function. Amino acids ; Structure of proteins ; Globular proteins ; Fibrous proteins ; Enzymes -- Unit II: Intermediary metabolism. Bioenergetics and oxidative phosphorylation ; Introduction to carbohydrates ; Glycolysis ; Tricarboxylic acid cycle ; Gluconeogenesis ; Glycogen metabolism ; Metabolism of monosaccharides and disaccharides ; Pentose phosphate pathway and NADPH ; Glycosaminoglycans and glycoproteins -- Unit III: Lipid metabolism. Metabolism of dietary lipids ; Fatty acid and triacylglycerol metabolism ; Complex lipid metabolism ; Cholesterol and steroid metabolism -- Unit IV: Nitrogen metabolism. Amino acids: disposal of nitrogen ; Amino acid degradation and synthesis ; Conversion of amino acids to specialized products ; Nucleotide metabolism -- Unit V: Integration of metabolism. Metabolic effects of insulin and glucagon ; The feed/fast cycle ; Diabetes mellitus ; Obesity ; Nutrition ; Vitamins -- Unit VI: Storage and expression of genetic information. DNA structure, replication and repair ; RNA structure, synthesis and processing ; Protein synthesis ; Regulation of gene expression ; Biotechnology and human disease.
  • 2011
    Harvey, Richard A.; Ferrier, Denise R.
    Status: Not Checked OutLane Catalog Record
    Protein structure and function -- Intermediary metabolism -- Lipid metabolism -- Nitrogen metabolism -- Integration of metabolism -- Storage and expression of genetic information.
  • 2003 Wiley
    Ardrey, R. E.
    ch. 1 Introduction, p. 1-5 -- ch. 2 Liquid Chromatography, p. 7-31 -- ch. 3 Mass Spectrometry, p. 33-74 -- ch. 4 Interface Technology, p. 75-127 -- ch. 5 Applications of High Performance Liquid Chromatography-Mass Spectrometry, p. 129-234.
  • 2011
    Lee, Peter Leader; Ferrell, James Ellsworth; Meyer, Tobias; Pfeffer, Suzanne R.; Theriot, Julie A.
    Membrane trafficking is an essential process that enables cells to deliver proteins to the extracellular space and segregate proteins into different compartments. Several steps are necessary for the functional segregation and processing of proteins. Trafficking includes post-translational modification of proteins, their movement between compartments, and stable localization once they have reached their target destinations. Importantly, membrane trafficking represents a recursively-sustained system: proteins that play a role in the regulation of membrane trafficking must also be properly transported and localized. Chapter one introduces the importance of the Golgi in the sorting and packaging of proteins and the roles of molecular motors in facilitating individual transport events. To modify transported cargoes, Golgi resident glycosyltransferases need to be localized to the correct cisternae and in some cases, in correct complex with other glycosyltransferases. "Kin recognition", the association of enzymatically compatible glycosyltransferases, has been demonstrated as a mechanism for the stable localization of glycosyltransferases to their proper compartments. Myosins, dyneins, and kinesins, linked to membranes by small Rab GTPases and their effectors, play important roles in moving cargo on actin and microtubule filaments. Their regulation by adapter proteins and their combinatorial interactions both complementing and opposing movement allows for the fine tuning of cargo transport. Chapter two presents the association of UDP-GlcNAc:[beta]Gal [beta]-1,3-N-acetylglucosaminyltransferase 1 (B3GNT1) and UDP-Gal:[beta]GlcNAc [beta]-1,4-galactosyltransferase, polypeptide 1 (B4GALT1). These two glycosyltransferases participate in the alternating, successive polymerization of poly-N-acetyllactosamine (polyLacNAc), a linear carbohydrate polymer composed of alternating N-acetylglucosamine and galactose residues. We show that these two glycosyltransferases colocalize by immunofluorescence microscopy, interact by coimmunoprecipitation, and when artificially retained in the ER can retain the complementary glycosyltransferase there as well. Chapter three presents the novel regulation of the KIF3 family kinesin, KIF1C, by small Rab GTPases, Rab1 and Rab6. The depletion of KIF1C impairs the plasma membrane delivery of Vesicular stomatitis virus G protein and intriguingly increases the speed of both Rab1 and Rab6 labeled vesicles. Rab6 can bind directly to the C-terminal domain of KIF1C and, like Rab1, can additionally interact with KIF1C's N-terminal motor domain. The interaction of KIF1C with Rab6 diminishes KIF1C's ability to bind microtubules. These results suggest that KIF1C acts as a brake, perhaps slowing transport to allow proper packaging and tethering to occur. Our data suggest that interaction between Rab6 and KIF1C could relieve KIF1C's braking action by displacing it from microtubules; alternatively, KIF1C may function as a molecular tether between membranes carrying Rab1 and/or Rab6.
  • 2013 Springer Protocols
    Bäckvall, Helena; Lehtiö, Janne.
    Part I. Low Molecular Weight Analysis Methods. Narrow-Range Peptide Isoelectric Focusing as Peptide Prefractionation Method Prior to Tandem Mass Spectrometry Analysis / Maria Pernemalm -- Analysis of Peptides by Denaturing Ultrafiltrationand LC-MALDI-TOF-MS / Yanming An and Radoslav Goldman -- Stable Isotope Labeling Methods in Protein Profiling / Johan Lengqvist and AnnSofi Sandberg -- Development of MRM-Based Assays for the Absolute Quantitation of Plasma Proteins / Michael A. Kuzyk, Carol E. Parker, Dominik Domanski, and Christoph H. Borchers -- Mass Spectrometric Profiling of Low-Molecular-Weight Proteins / Matthias Rainer, Constantin Sajdik, and Günther K. Bonn -- Developing an iMALDI Method / Brinda Shah, Jennifer D. Reid, Michael A. Kuzyk, Carol E. Parker, and Christoph H. Borchers -- Analysis of Neuropeptides by MALDI Imaging Mass Spectrometry / Anna Ljungdahl, Jörg Hanrieder, Jonas Bergquist, and Malin Andersson -- Highly Multiplexed Antibody Suspension Bead Arrays for Plasma Protein Profiling / Kimi Drobin, Peter Nilsson, and Jochen M. Schwenk -- Part II. Bioinformatics Of Proteomics Data. Protein Quantification by Peptide Quality Control (PQPQ) of Shotgun Proteomics Data / Jenny Forshed -- Part III Methods Applied In Clinical Research. Collection and Handling of Blood Specimens for Peptidomics / Harald Tammen and Rudiger Hess -- An Automated RP-SCX Solid-Phase Extraction Procedure for Urinary Peptidomics Biomarker Discovery Studies / Crina I.A. Balog, Rico Derks, Oleg A. Mayboroda, and André M. Deelder -- Application of Phage Display for Ligand Peptidomics to Identify Peptide Ligands Binding to AQP2-Expressing Membrane Fractions / Byung-Heon Lee and Tae-Hwan Kwon -- Protein Expression Profiling of Brain Tumor Tissue Using SELDI-MS / Carl Wibom -- Comprehensive Analysis of MHC Ligands in Clinical Material by Immunoaffinity-Mass Spectrometry / Kie Kasuga.
  • 2006 Springer
    Tsoukas, Constantine.
    Protein kinase C-theta (PKC[theta]): new perspectives on its functions in T-cell biology / Rina Barouch-Bentov and Amnon Altman -- Travel informations on the tec kinases during lymphocyte activation / Fabien Garçon and Jacques A. Nunès -- Inducible T-cell tyrosine kinase (ITK): structural requirements and actin polymerization / Constantine D. Tsoukas ... [et al.] -- Differential requirements of PI3K subunits for BCR or BCR/CD19-induced ERK activation / Elena Vigorito and Martin Turner -- Protein tyrosine phosphatases in human disease / Tomas Mustelin -- The T-cell-specific adapter protein functions as a regulator of peripheral but not central immunological tolerance / Philip E. Lapinski ... [et al.] -- Proximal signals controlling B-cell antigen receptor (BCR) mediated NF-[kappa]B activation / Miguel E. Moreno-García ... [et al.] -- The adapter 3BP2: how it plugs into leukocyte signaling / Marcel Deckert and Robert Rottapel -- CTLA-4 regulation of T-cell function via RAP-1-mediated adhesion / Helga Schneider ... [et al.] -- Protein crosstalk in lipid rafts / Raquel J. Nunes, Mónica A.A. Castro, and Alexandre M. Carmo -- Role of lipid rafts in activation-induced cell death: the fas pathway in aging / Anis Larbi ... [et al.] -- T-cell response in aging: influence of cellular cholesterol modulation / Tamàs Fülöp ... [et al.] -- Lymphocyte signaling and the translatability of mRNA / Suzanne Miyamoto -- Protein arginine methylation: a new frontier in T-cell signal transduction / Brandon T. Schurter, Fabien Blanchet, and Oreste Acuto -- Immune regulation by ubiquitin conjugation / K. Venuprasad ... [et al.] -- CTIB (C-terminus protein of I[kappa]B-[beta]): a novel factor required for acidic adaptation /Toshiko Fukamachi ... [et al.] -- HIV may deplete most CD4 lymphocytes by a mechanism involving signaling through its receptors on non-permissive resting lymphocytes / Miles W. Cloyd ... [et al.] -- Integrating traditional and postgenomic approaches to investigate lymphocyte development and function / Yina Hsing Huang ... [et al.] -- Phosphoproteomic analysis of lymphocyte signaling / Lulu Cao, Kebing Yu, and Arthur R. Salomon.
    Also available: Print – 2006
  • 2015 ScienceDirect
    Platt, Frances M.; Platt, Nick.
  • 2014 Springer
    Anderluh, Gregor; Gilbert, Robert.
    Introduction: brief historical overview -- Distribution of MACPF/CDC proteins -- Evolution of the complement system -- Structural features of cholesterol dependent cytolysins and comparison to other MACPF-domain containing proteins -- Perfringolysin O structure and mechanism of pore formation as a paradigm for cholesterol-dependent cytolysins -- Structural biology of the membrane attack complex -- Membrane interactions and cellular effects of MACPF/CDC proteins -- The biology of pneumolysin -- Multifaceted activity of listeriolysin O, the cholesterol -dependent cytolysin of Listeria monocytogenes -- Perforin: a key pore-forming protein for immune control of viruses and cancer -- Perforin and human diseases -- The role of MACPF proteins in the biology of malaria and other apicomplexan parasites -- Chlamydial MACPF protien CT153 -- Fungal MACPF-like proteins and aegerolysins: bi-component pore-forming proteins? -- Fluorescence imaging of MACPF/CDC proteins: new techniques and their application.
  • 2015 Springer Protocols
    Sun, Xue-Long.
    Synthesis of chain-end functionalized glycopolymers via cyanoxyl-mediated free radical polymerization (CMFRP) / Valentinas Gruzdys ... [et al.] -- Protecting-group-free synthesis of well-defined glycopolymers featuring negatively charged oligosaccharides / Luca Albertin -- Glycopolymers prepared by ring-opening metathesis polymerization followed by glycoconjugation using a triazole-forming "click" reaction / Ronald Okoth and Amit Basu -- Protecting-group-free synthesis of glycopolymers and their binding assay with lectin and influenza virus / Tomonari Tanaka, Tadanobu Takahashi, and Takashi Suzuki -- Carbohydrate-based initiators for the cationic ring-opening polymerization of 2-Ethyl-2-Oxazoline / Christine Weber ... [et al.] -- Heterofunctional glycopolypeptides by combination of Thiol-Ene chemistry and NCA polymerization / Kai-Steffen Krannig and Helmut Schlaad -- Preparation of proteoglycan mimetic graft copolymers / Matt J. Kipper and Laura W. Place -- Galactosylated polymer nano-objects by polymerization-induced self-assembly, potential drug nanocarriers / Mona Semsarilar, Irene Canton, and Vincent Ladmiral -- Synthetic approach to biotinylated glyco-functionalized quantum dots : a new fluorescent probes for biomedical applications / Christian K. Adokoh, James Darkwa, and Ravin Narain -- Surface modification of polydivinylbenzene microspheres with a fluorinated glycopolymer using Thiol-Halogen click chemistry / Wentao Song and Anthony M. Granville -- Glycopolymer-grafted polymer particles for lectin recognition / Michinari Kohri, Tatsuo Taniguchi, and Keiki Kishikawa -- Synthesis of non-spherical glycopolymer-decorated nanoparticles : combing Thiol-ene with catecholic chemistry / Xiao Li, Weidong Zhang, and Gaojian Chen -- Synthetic approach to glycopolymer base nanoparticle gold(I) conjugate : a new generation of therapeutic agents / Christian K. Adokoh, James Darkwa, and Ravin Narain -- Multivalent glycopolymer-coated gold nanoparticles / Sarah-Jane Richards ... [et al.] -- Modulation of multivalent protein binding on surfaces by glycopolymer brush chemistry / Kai Yu ... [et al.] -- Oriented immobilized sialyloligo-macroligand microarray / Satya Nandana Narla and Xue-Long Sun -- Glycocalyx remodeling with glycopolymer-based proteoglycan mimetics / Mia L. Huang ... [et al.].
  • 2012 Springer
    Carrondo, Maria Arménia; Spadon, Paola.
  • 2011
    Weber, Stephanie Crane; Kaiser, A. Dale; Shapiro, Lucy; Spakowitz, Andrew; Straight, Aaron; Theriot, Julie A.
    The cellular interior is crowded with macromolecules that form highly organized but dynamic structures. While these properties of the cytoplasm have become well known, their physical consequences on intracellular processes such as transport and gene expression are still not well understood. The goal of this dissertation is to begin to elucidate these physical consequences and to develop a new model for macromolecular motion in vivo. Tracking of fluorescently labeled chromosomal loci in live bacterial cells reveals a robust subdiffusive scaling of the mean square displacement (MSD). To identify the source of anomalous behavior, we developed analytical theory and Brownian dynamics simulations of polymers under various physical environments. Specifically, we examined the consequences of confinement, self-interaction, viscoelasticity and random waiting on monomer motion. We found that neither confinement nor self-interaction alter the fundamental Rouse mode relaxations of a polymer. However, viscoelasticity, modeled by fractional Langevin motion (fLm), and random waiting, modeled with a continuous time random walk (CTRW), led to significant and distinct deviations from the classic polymer dynamics model. We propose that the subdiffusive motion of chromosomal loci observed in vivo arises from relaxation of the Rouse modes of the DNA polymer within the viscoelastic environment of the cytoplasm. The time-averaged and ensemble-averaged MSD of chromosomal loci exhibit ergodicity, and the velocity autocorrelation function is negative at short time lags. These observations are most consistent with fLm and rule out a CTRW model as an explanation for anomalous motion in vivo. In contrast to the subdiffusive scaling exponent, the apparent diffusion coefficient is variable across species and sensitive to biological perturbations. When ATP synthesis is inhibited in E. coli, the apparent diffusion coefficient decreases 2-fold while the scaling exponent remains constant. This observation suggests that metabolic activity contributes to the magnitude of locus motion, but does not affect the subdiffusive mechanism (or the viscoelastic properties of the cytoplasm). Furthermore, the temperature-dependence of locus motion is steeper in untreated cells than in ATP-depleted cells. These results suggest that untreated cells have an additional source of molecular agitation that increases with temperature. Such ATP-dependent fluctuations are likely mechanical, as the heat dissipated from glycolysis and oxidative phosphorylation is insufficient to account for the difference in the apparent diffusion coefficient between untreated and ATP-depleted cells. We are currently investigating the dynamic contributions of several molecular candidates. Our data indicate that ATP-dependent enzymatic activity, in addition to thermal fluctuations, may contribute to the molecular agitation driving (sub)diffusion in the cell.
  • 2010 Springer Protocols
    Seger, Rony.
    The MAP kinase signaling cascades : A system of hundreds of components regulates a diverse array of physiological functions -- Determination of ERK activity: Anti-phospho-ERK antibodies and in vitro phosphorylation -- Activation of SAPK/JNKs in vitro -- Activation of p38 and determination of its activity -- Activity assays for extracellular signal-regulated kinase 5 -- Use of inhibitors in the study of MAP kinases -- MAP kinase activation by receptor tyrosine kinases : in control of cell migration -- Activation of ras and rho GTPases and MAP kinases by G-protein-coupled receptors -- Regulation of MAP kinase signaling by calcium -- Identification of novel substrates of MAP kinase cascades using bioengineered kinases that uniquely utilize analogs of ATP to phosphorylate substrates -- ERK-MAP kinase signaling in the cytoplasm -- Lentiviral vectors to study the differential function of ERK1 and ERK2 MAP kinases -- Structural studies of MAP kinase cascade components -- Analysis of MAP kinases by hydrogen exchange mass spectrometry -- A "Molecular evolution" approach for isolation of intrinsically active (MEK-independent) MAP kinases -- Reconstitution of the nuclear transport of the MAP kinase ERK2 -- Localization and trafficking of fluorescently tagged ERK1 and ERK2 -- Studying the regulation of MAP kinase by MAP kinase phosphatases in vitro and in cell systems -- Proteomic analysis of scaffold proteins in the ERK cascade -- Analysis of ERKs' dimerization by electrophoresis -- MAP kinase: SUMO pathway interactions -- Computational modelling of kinase signalling cascades -- Analysis of mitogen-activated protein kinase activity in yeast -- Detection of RTK pathway activation in drosophila using anti-dpERK immunofluorescence staining -- Studying MAP kinase pathways during early development of xenopus laevis -- Deciphering signaling pathways in vivo : The ras/raf/MEK/ERK cascade -- Mutational and functional analysis in human ras/MAP kinase genetic syndromes -- Implication of the ERK pathway on the post-transcriptional regulation of VEGF mRNA stability -- Studies on MAP kinase signaling in the immune system -- Methods to study MAP kinase signalling in the central nervous system -- MAP kinase regulation of the mitotic spindle checkpoint -- Using high-content microscopy to study gonadotrophin-releasing hormone regulation of ERK.
  • 2013 Springer Protocols
    Matthiesen, Rune.
    Since the publishing of the first edition, the methodologies and instrumentation involved in the field of mass spectrometry-based proteomics has improved considerably. Fully revised and expanded, Mass Spectrometry Data Analysis in Proteomics, Second Edition presents expert chapters on specific MS-based methods or data analysis strategies in proteomics. The volume covers data analysis topics relevant for quantitative proteomics, post translational modification, HX-MS, glycomics, and data exchange standards, among other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and authoritative, Mass Spectrometry Data Analysis in Proteomics, Second Edition serves as a detailed guide for all researchers seeking to further our knowledge in the field of proteomics.
  • 2006 Springer Protocols
    Matthiesen, Rune.
  • 2014 Springer Protocols
    Raftery, Daniel.
    Overview of mass spectrometry-based metabolomics : opportunities and challenges -- Global metabolic profiling using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry -- LC-MS profiling to link metabolic and phenotypic diversity in plant mapping populations -- Mitochondrial metabolomics using high-resolution Fourier-transform mass spectrometry -- Sample preparation methods for LC-MS-based global aqueous metabolite profiling -- Methods of discovery-based and targeted metabolite analysis by comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry detection -- Analysis of mouse liver metabolites by GC x GC-TOF MS -- Metabolite fingerprinting by capillary electrophoresis-mass spectrometry -- Quantitative metabolomic profiling using dansylation isotope labeling and liquid chromatography mass spectrometry -- Quantitative analysis of amino and organic acids by methyl chloroformate derivatization and GC-MS/MS analysis -- Stable isotope-labeled tracers for metabolic pathway elucidation by GC-MS and FT-MS -- Multiplexed, quantitative, and targeted metabolite profiling by LC-MS/MRM -- Multidimensional mass spectrometry-based shotgun lipidomics -- Comprehensive quantitative determination of PUFA-related bioactive lipids for functional lipidomics using high-resolution mass spectrometry -- Ultra-performance liquid chromatography-mass spectrometry targeted profiling of bile acids : application to serum, liver tissue, and cultured cells of different species -- Analysis of volatile organic compounds in exhaled breath by gas chromatography-mass spectrometry combined with chemometric analysis -- Headspace SPME-GC-MS metabolomics analysis of urinary volatile organic compounds (VOCs) -- Metabolite profiling by direct analysis in real-time mass spectrometry -- Analysis of dried blood spots using DESI mass spectrometry -- DESI-MS imaging of lipids and metabolites from biological samples -- Metabolic imaging using nanostructure-initiator mass spectrometry (NIMS) -- Statistical analysis and modeling of mass spectrometry-based metabolomics data.
  • 2013 Springer Protocols
    Kohler, Jennifer; Patrie, Steven M.
    Introduction to glycosylation and mass spectrometry / Steven M. Patrie, Michael J. Roth, and Jennifer J. Kohler -- PART I ENRICHMENT AND ISOLATION METHODS -- Tandem lectin weak affinity chromatography for glycoprotein enrichment / Zhi Yuan Ma, Yuliya Skorobogatko, and Keith Vosseller -- CSC technology: Selective labeling of glycoproteins by mild oxidation to phenotype cells / Andreas Hofmann, Damaris Bausch-Fluck, and Bernd Wollscheid -- Use of boronic acid nanoparticles in glycoprotein enrichment / Yawei Xu, Lijuan Zhang, and Haojie Lu -- Incorporation of unnatural sugars for the identification of glycoproteins / Balyn W. Zaro, Howard C. Hang, and Matthew R. Pratt -- Characterization of membrane-associated glycoproteins using lectin affinity chromatography and mass spectrometry / Yashu Liu, Jintang He, and David M. Lubman -- Sialic acid capture-and-release and LC-MS(n) analysis of glycopeptides / Jonas Nilsson and Göran Larson -- PART II SAMPLE PREPARATION -- In-solution digestion of glycoproteins for glycopeptide-based mass analysis / Eden P. Go, Kathryn R. Rebecchi, and Heather Desaire -- Nano-HPLC-MS of glycopeptides obtained after nonspecific proteolysis / Gerhild Zauner, Carolien A.M. Koeleman, André M. Deelder, and Manfred Wuhrer -- PART III SEPARATION METHODS -- Glycopeptide enrichment for MALDI-TOF mass spectrometry analysis by hydrophilic interaction liquid chromatography solid phase extraction (HILIC SPE) / Pia Hønnerup Jensen, Simon Mysling, Peter Højrup, and Ole Nørregaard Jensen -- Separation and identification of glycoforms by capillary electrophoresis with electrospray ionization mass spectrometric detection / Alina D. Zamfir, Corina Flangea, Alina Serb, Ana-Maria Zagrean, Andreas M. Rizzi, and Eugen Sisu -- Structural separations by ion mobility-MS for glycomics and glycoproteomics / Larissa S. Fenn and John A. McLean -- PART IV QUANTITATION METHODS -- Quantitative analysis of glycoprotein glycans / Ron Orlando -- Stable isotope labeling of N-glycosylated peptides by enzymatic deglycosylation for mass spectrometry-based glycoproteomics / Hiroyuki Kaji and Toshiaki Isobe -- Approaches for site mapping and quantification of o-linked glycopeptides / Peng Zhao, Stephanie H. Stalnaker, and Lance Wells -- Glycan profiling: Label-free analysis of glycoproteins / Yoshinao Wada -- PART V COMPUTATIONAL TOOLS -- Introduction to informatics in glycoprotein analysis / Kiyoko F. Aoki-Kinoshita -- Software tools for glycan profiling / Chuan-Yih Yu, Anoop Mayampurath, and Haixu Tang -- PART VI CASE STUDIES IN MASS SPECTROMETRY OF GLYCOPROTEINS -- Quantitative characterization of glycoproteins in neurodegenerative disorders using iTRAQ / Min Shi, Hyejin Hwang, and Jing Zhang -- Quantitative proteomic analysis of N-linked glycoproteins in human tear fluid / Lei Zhou and Roger W. Beuerman -- Elucidation of N-glycosites within human plasma glycoproteins for cancer biomarker discovery / Penelope Drake, Birgit Schilling, Brad Gibson, and Susan Fisher -- Characterizing the glycosylation state of therapeutic recombinant glycoproteins. / Nicole Samuels, David Kates, Jun Liu, and Joanne Severs.
  • 2009 Springer Protocols
    Lipton, Mary S.; Paša-Tolić, Ljiljana.
    Generation and analysis of multidimensional protein identification technology datasets / Selene K. Swanson, Laurence Florens, and Michael P. Washburn -- Quantitative peptide and protein profiling by mass spectrometry / Alexander Schmidt, Birgit Bisle, and Thomas Kislinger -- Label-free relative quantitation of prokaryotic proteomes using the accurate mass and time tag approach / Kim K. Hixson -- Classical proteomics: two-dimensional electrophoresis/MALDI mass spectrometry / Ursula Zimny-Arndt ... [et al.] -- The use of difference in-gel electrophoresis for quantitation of protein expression / Rajat Sapra -- Liquid-chromatography-mass spectrometry of thylakoid membrane proteins / Christian G. Huber ... [et al.] -- High accuracy mass spectrometry in large-scale analysis of protein phosphorylation / Jesper V. Olsen and Boris Macek -- Manual validation of peptide sequence and sites of tyrosine phosphorylation from MS/MS spectra / Amy M. Nichols and Forest M. White -- Assigning glycosylation sites and microheterogeneities in glycoproteins by liquid chromatography/tandem mass spectrometry / Yehia Mechref, Milan Madera, and Milos V. Novotny -- Structure analysis of N -glycoproteins / Stefanie Henning, Jasna Peter-Katalinicʹ, and Gottfried Pohlentz -- Capillary zone electrophoresis-mass spectrometry for the characterization of isoforms of intact glycoproteins / Christian Neusüss, and Matthias Pelzing -- Top-down proteomics on a high-field fourier transform ion cyclotron resonance mass spectrometer / Séverine A. Ouvry-Patat ... [et al.] -- Capillary isoelectric focusing/reversed phase liquid chromatography/mass spectrometry / Cheng S. Lee and Brian M. Balgley -- Integrating accelerated tryptic digestion into proteomics workflows / Gordon W. Slysz and David C. Schriemer -- Hydrogen/deuterium exchange mass spectrometry / Xuguang Yan and Claudia S. Maier -- Mass spectrometry detection and characterization of noncovalent protein complexes / Sheng Yin and Joseph A. Loos -- Chemical cross-linking for protein-protein interaction studies / Xiaoting Tang and James E. Bruce -- Tissue analysis with high-resolution imaging mass spectrometry / A.F. Maarten Altelaar and Ron M.A. Heeren -- Proteomic global profiling for cancer biomarker discovery / Vitor Faca, Hong Wang, and Samir Hanash -- Analysis of protein glycosylation and phosphorylation using liquid phase separation, protein microarray technology, and mass spectrometry / Jia Zhao ... [et al.] -- Transthyretin mass determination for detection of transthyretin familial amyloid / John F. O'Brien and H. Robert Bergen III -- Characterization of microorganisms by MALDI mass spectrometry / Catherine E. Petersen, Nancy B. Valentine, and Karen L. Wahl -- Mass spectrometric characterization of neuropeptides / Stephanie S. DeKeyser, James A. Dowell, and Lingjun Li -- Peptide and protein ion/ion reactions in electrodynamic ion traps: tools and methods / Scott A. McLuckey -- Electron capture dissociation LC/MS/MS for bottom-up proteomics / Roman A. Zubarev -- Two-dimensional ion mobility analyses of proteins and peptides / Alexandre A. Shvartsburg, Keqi Tang, and Richard D. Smith -- Proteomics for validation of automated gene model predictions / Kemin Zhou ... [et al.] -- Support vector machines for improved peptide identification from tandem mass spectrometry database search / Bobbie-Jo M. Webb-Robertson.
  • 2012 Springer
    Gupta, Satya Prakash.
    Matrix Metalloproteinases / Viola Vargová, Marek Pytliak and Viola Mechírová -- Specificity of Binding with Matrix Metalloproteinases / Satya P. Gupta and Vaishali M. Patil -- The Gelatinases and Their Inhibitors: The Structure-Activity Relationships / Snehasikta Swarnakar, Amartya Mishra and Susri Ray Chaudhuri (Guha) -- Advances in Studies on Collagenase Inhibitors / Mange Ram Yadav, Prashant Revan Murumkar and Vishal Prakash Zambre -- Hydroxamic Acids as Matrix Metalloproteinase Inhibitors / Rajeshwar P. Verma -- Quantitative Structure-Activity Relationship Studies on Sulfonamide-Based MMP Inhibitors / Vaishali M. Patil and Satya P. Gupta -- Matrix Metalloproteinase Inhibitors as Investigative Tools in the Pathogenesis and Management of Vascular Disease / Mina M. Benjamin and Raouf A. Khalil.
  • 2008 Springer
    Boichot, Elisabeth; Lagente, Vincent.
    Also available: Print – 2008
  • 2014 Springer
    Carlberg, Carsten; Molnár, Ferdinand.
    This textbook aims to describe the fascinating area of eukaryotic gene regulation for graduate students in all areas of the biomedical sciences. Gene expression is essential in shaping the various phenotypes of cells and tissues and as such, regulation of expression is a fundamental aspect of nearly all processes in physiology, both in healthy and in diseased states. This pivotal role for the regulation of gene expression makes this textbook essential reading from students of all the biomedical sciences in order to be better prepared for their specialized disciplines. A complete understanding of transcription factors and the processes that alter their activity is a major goal of modern life science research. The availability of the whole human genome sequence (and that of other eukaryotic genomes) and the consequent development of next-generation sequencing technologies have significantly changed nearly all areas of the biological sciences. For example, the genome-wide location of histone modifications and transcription factor biding sites, such as provided by the ENCODE consortium, has greatly improved our understanding of gene regulation. Therefore, the focus of this book is the description of the post-genome understanding of gene regulation. The purpose of this book is to provide, in a condensed form, an overview on the present understanding of the mechanisms of gene regulation.
  • 2008 Springer
    Kamkin, A. G.; Kiseleva, Irina.
  • 2011 Springer
    Kamkin, A. G.; Kiseleva, Irina.
  • 2014 ClinicalKey
    Baynes, John W.; Dominiczak, Marek H.
    Brought to you in a thorough yet accessible manner, the new edition of Medical Biochemistry gives access to all of the latest information on basic and clinically focused genetic and molecular biology. Featuring a team of contributors that includes investigators involved in cutting-edge research as well as experienced clinicians, this updated medical textbook offers a unique combination of both research and practice that's ideal for today's problem-based integrated courses. Relate biochemistry to everyday practice with the help of Clinical Boxes integrated into the text, and access in-depth coverage of important topics - including recent research in biochemistry - through Advanced Concept Boxes.Test your knowledge and improve retention with Active Learning Boxes at the conclusion of each chapter, and quickly review the most common lab tests performed with convenient Clinical Test Boxes.Effectively study the most updated information in biochemistry with the help of a dynamic, full-color design.Better understand the relationship between science and clinical practice with material organized by organ rather than system.
  • 2002 ScienceDirect
    Bhagavan, N. V.
    Also available: Print – 2002
  • 2010 Springer
    Catania, Anna.
    Structure-activity relationships (SAR) of melanocortin and agouti-related (AGRP) peptides / Anamika Singh, Erica M. Haslach and Carrie Haskell-Luevano -- Melanocortin signalling mechanisms / Paula C. Eves and John W. Haycock -- Distribution and function of melanocortin receptors within the brain / Kathleen G. Mountjoy -- Drugs, exercise, and the melanocortin-4 receptor: different means, same ends: treating obesity / Jay W. Schaub, Erin B. Bruce, and Carrie Haskell-Luevano -- Melanocortins in brain inflammation: the role of melanocortin receptor subtypes / Ruta Muceniece and Maija Dambrova -- Melanocortins and the cholinergic anti-inflammatory pathway / Daniela Giuliani ... [et al.] -- Melanocortin control of cell trafficking in vascular inflammation / Hetal B. Patel ... [et al.] -- Terminal signal: anti-inflammatory effects of α-melanocyte-stimulating hormone related peptides beyond the pharmacophore / Thomas Brzoska ... [et al.] -- Protective effects of melanocortins in systemic host reactions / Stefano Gatti ... [et al.] -- Development of α-melanocortin analogs for melanoma prevention and targeting / Zalfa A. Abdel-Malek -- MSH radiopeptides for targeting melanoma metastases / Alex N. Eberle ... [et al.] -- Applications of the role of α-MSH in ocular immune privilege / Andrew W. Taylor and Darren Lee.
    Also available: Print – 2010
  • 2015 Springer
    Disalvo, E. Anibal.
    1. Membrane hydration: a hint to a new model for biomembranes -- 2. Use of x-ray and neutron scattering methods with volume measurements to determine lipid bilayer structure and number of water molecules/lipid -- 3. Water and lipid bilayers -- 4. Hydration forces between lipid bilayers: a theoretical overview and a look on methods exploring dehydration -- 5. Monitoring membrane hydration with 2-(dimethylamino)-6-acylnaphtalenes fluorescent probes -- 6. Long-range lipid-water interaction as observed by ATR-FTIR spectroscopy -- 7. Hydration and nanoconfined water: insights from computer simulations -- 8. Aquaphotomics: near infrared spectoscopy and water states in biological systems -- 9. Hydration in lipid monolayers: correlation of water activity and surface pressure -- 10. Water at biological phase boundaries: its role in interfacial activation of enzymes and metabolic pathways -- 11. Anhydrobiosis: an unsolved problem with applications in human welfare -- Concluding remarks -- Index.
  • 2003. ScienceDirect
    Mattson, Mark Paul.
  • 2002 ScienceDirect
    Jagow, Gebhard von; Schägger, Hermann.
  • 2012 Springer Protocols
    Klein-Seetharaman, Judith; Vaidehi, Nagarajan.
    Crystallization of membrane proteins in bicelles -- Vapor diffusion-controlled meso crystallization of membrane proteins -- Solution NMR studies of integral polytopic alpha-helical membrane proteins: The structure determination of the seven-helix transmembrane receptor sensory rhodopsin ii, psrii -- Use of NMR saturation transfer difference spectroscopy to study ligand binding to membrane proteins -- How to investigate interactions between membrane proteins and ligands by solid-state NMR -- Identifying and measuring transmembrane helix-helix interactions by FRET -- Studying substrate binding to reconstituted secondary transporters by attenuated total reflection infrared difference spectroscopy -- UV-visible and infrared methods for investigating lipid-rhodopsin membrane interactions -- Proteomic characterization of integral membrane proteins using thermostatted liquid chromatography coupled with tandem mass spectrometry -- LITiCon: A discrete conformational sampling computational method for mapping various functionally selective conformational states of transmembrane helical proteins -- Homology model-assisted elucidation of binding sites in GPCRs -- Comparative modeling of lipid receptors -- Quantification of structural distortions in the transmembrane helices of GPCRs -- Structure prediction of g protein-coupled receptors and their ensemble of functionally important conformations -- Target based virtual screening by docking into automatically generated GPCR models -- Predicting the biological activities through QSAR analysis and docking-based scoring -- Identification of motions in membrane proteins by elastic network models and their experimental validation -- Modeling the structural communication in supramolecular complexes involving GPCRs -- Exploring substrate diffusion in channels using biased molecular dynamics simulations.
  • 2013 Springer Protocols
    Ghirlanda, Giovanna; Senes, Alessandro.
    Use of Thiol-Disulfide Exchange Method to Study Transmembrane Peptide Association in Membrane Environments -- Measurement of Transmembrane Peptide Interactions in Liposomes Using Fr ster Resonance Energy Transfer (FRET) -- Measuring Transmembrane Helix Interaction Strengths in Lipid Bilayers Using Steric Trapping -- Genetics Systems for Monitoring Interactions of Transmembrane Domains in Bacterial Membranes -- Analyzing the Effects of Hydrophobic Mismatch on Transmembrane -Helices Using Tryptophan Fluorescence Spectroscopy -- Folding Alpha Helical Membrane Proteins into Liposomes In Vitro and Determination of Secondary Structure -- Solvation Models and Computational Prediction of Orientations of Peptides and Proteins in Membranes -- Membrane Protein Structure Determination: Back to the Membrane -- On the Role of NMR Spectroscopy for Characterization of Antimicrobial Peptides -- Prediction and Design of Outer Membrane Protein-Protein Interactions -- Design of Transmembrane Peptides: Coping with Sticky Situations -- Engineering and Utilization of Reporter Cell Lines for Cell-Based Assays of Transmembrane Receptors -- Fluorination in the Design of Membrane Protein Assemblies.
  • 2015 ScienceDirect
    Shukla, Arun K.
  • 2015 ScienceDirect
    Shukla, Arun K.
    Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.
  • 2010 Springer
    Ward, Jeremy P. T.; Yuan, Jason X.-J.
    The role of ion channels in hypoxic pulmonary vasoconstriction / E. Kenneth Weir ... [et al.] -- Two-pore domain K channels and their role in chemoreception / Keith J. Buckler -- Intricate interaction between store-operated calcium entry and calcium-activated chloride channels in pulmonary artery smooth muscle cells / Abigail S. Forrest ... [et al.] -- The role of intracellular ion channels in regulating cytoplasmic calciumin pulmonary arterial mmooth [i.e. smooth] muscle : which store and where? / A. Mark Evans -- Ca2 oscillations regulate contraction of intrapulmonary smooth muscle cells / Michael J. Sanderson, Yan Bai, and Jose Perez-Zoghbi -- Introduction to TRP channels : structure, function, and regulation / Michael Y. Song and Jason S.-J. Yuan -- Physiological functions of transient receptor potential channels in pulmonary arterial smooth muscle cells / Xiao-Ru Yang, Mo-Jun Lin, and James S.K. Sham -- The contribution of TRPC1 and STIM1 to capacitative Ca2 entry in pulmonary artery / Lih Chyuan Ng, Judith A. Airey, and Joseph R. Hume -- Store-operated calcium entry channels in pulmonary endothelium : the emerging story of TRPCS and Orai1 / Donna L. Cioffi, Christina Barry, and Troy Stevens -- TRPM2 channel regulates endothelial barrier function / Claudie M. Hecquet, Gias U. Ahmmed, and Asrar B. Malik -- A proposed mitochondrial-metabolic mechanism for initiation and maintenance of pulmonary arterial hypertension in fawn-hooded rats : the Warburg model of pulmonary arterial hypertension / Jalees Rehman and Stephen L. Archer -- The role of classical transient receptor potential channels in the regulation of hypoxic pulmonary vasoconstriction / B. Fuchs ... [et al.] -- Developmental regulation of oxygen sensing and ion channels in the pulmonary vasculature / David N. Cornfield -- Hypoxic regulation of ion channels and transporters in pulmonary vascular smooth muscle / Larissa A. Shimoda -- CLC-3 chloride channels in the pulmonary vasculature / Joseph R. Hume ... [et al.] -- Role of bone morphogenetic protein receptors in the development of pulmonary arterial hypertension / Nicholas W. Morrell -- Cross talk between Smad, MAPK, and actin in the etiology of pulmonary arterial hypertension / James West -- Notch signaling in pulmonary hypertension / Patricia A. Thistlethwaite, Xiaodong Li, and Xiaoxue Zhang -- Rho kinase-mediated vasoconstriction in pulmonary hypertension / Ivan F. McMurtry ... [et al.] -- The serotonin hypothesis of pulmonary hypertension revisited / Margaret R. MacLean and Yvonne Dempsie -- Impaired vascular endothelial growth factor signaling in the pathogenesis of neonatal pulmonary vascular disease / Steven H. Abman -- Mitochondrial regulation of oxygen sensing / Navdeep S. Chandel -- Reactive oxygen species and RhoA signaling in vascular smooth muscle : role in chronic hypoxia-induced pulmonary hypertension / Thomas C. Resta, Brad R.S. Broughton, and Nikki L. Jernigan -- Polyamine regulatory pathways as pharmacologic targets in pulmonary arterial hypertension / Mark N. Gillespie and Jack W. Olson -- 5-HT receptors and Kv channel internalization / Angel Cogolludo and Francisco Perez-Vizcaino -- KCNQ potassium channels : new targets for pulmonary vasodilator drugs? / Alison M. Gurney, Shreena Joshi, and Boris Manoury -- Receptor tyrosine kinase inhibitors in rodent pulmonary hypertension / Liliana Moreno-Vinasco and Joe G.N. Garcia -- PDGF receptor and its antagonists : role in treatment of PAH / Friedrich Grimminger and Ralph Theo Schermuly -- PPAR[gamma] and the pathobiology of pulmonary arterial hypertension / Marlene Rabinovitch -- Targeting TASK-1 channels as a therapeutic approach / Andrea Olschewski -- Pharmacological targets for pulmonary vascular disease : vasodilation versus anti-remodelling / Matthew Thomas.
    Also available: Print – 2010
  • 2015 ScienceDirect
    Metallo, Christian M.
  • 2016 Springer
    Herzig, Stephan.
    The HEP issue on Metabolic Control provides a state-of the art overview over both classical concepts and emerging areas in metabolism and associated disorders. In this context, metabolic control is highlighted at various levels of complexity ranging from transcriptional mechanisms in metabolic pathway control over metabolic communication routes in physiology and pathophysiology to current treatment modalities and options in metabolic disorders, including type 2 diabetes and obesity. Dedicated chapters by leading experts in their fields provide a concise overview over important areas in metabolic research at a molecular level, including the role of the central nervous system in metabolism, inflammation and metabolism, pancreatic hormone signaling, brown adipose tissue, and therapeutic concepts.
  • 2001 ScienceDirect
    Aizawa, Hiroyasu; Aizawa, Hiroyasu.
    Acid amides -- Amidines, guanidines, and hydrazines -- Amino acids: phosphinic acids -- Anilines and nitrobenzenes -- Aryloxy acids -- Biphenyl ethers -- Carbamates -- Dithio- and thiolcarbamates -- Halogenated aliphatics -- Halogenated aromatics -- Five-membered heterocycles -- Five-membered heterocycles (fused) -- Six-/more membered heterocycles -- Six-/more membered heterocycles (fused) -- Imides -- Natural products -- Organophosphorous compounds -- Oximes -- Phenylureas and related compounds -- Phosphono amino acids and related compounds -- Pyrethroids -- Sulfonylureas -- Triazines and related compounds -- Miscellaneous.
  • 2011 Springer Protocols
    Metz, Thomas O.
    Origins of metabolic profiling / Arthur B. Robinson and Noah E. Robinson -- Amino acid profiling for the diagnosis of inborn errors of metabolism / Monique Piraud ... [et al.] -- Acylcarnitines : analysis in plasma and whole blood using tandem mass spectrometry / David S. Millington and Robert D. Stevens -- Analysis of organic acids and acylglycines for the diagnosis of related inborn errors or metabolism by GC- and HPLC-MS / Giancarlo la Marca and Cristiano Rizzo -- HPLC analysis for the clinical-biochemical diagnosis of inborn errors of metabolism of purines and pyrimidines / Giuseppe Lazzarino ... [et al.] -- Bile acid analysis in various biological samples using ultra performance liquid chromatography/electrospray ionization-mass spectrometry (UPLC/ESI-MS) / Masahito Hagio, Megumi Matsumoto, and Satoshi Ishizuka -- Analysis of glycolytic intermediates with ion chromatography- and gas chromatography-mass spectrometry / Jan C. van Dam, Cor Ras, and Angela ten Pierick -- Analysis of the citric acid cycle intermediates using gas chromatography-mass spectrometry / Rajan S. Kombu, Henri Brunengraber, and Michelle A. Puchowicz -- Quantification of pentose phosphate pathway (PPP) metabolites by liquid chromatography-mass spectrometry (LC-MS) / Amber Jannasch, Miroslav Sedlak, and Jiri Adamec -- High-performance liquid chromatography-mass spectrometry (HPLC-MS)-based drug metabolite profiling / Ian D. Wilson -- Gas chromatography-mass spectrometry (GC-MS)-based metabolomics / Antonia Garcia and Coral Barbas -- Use of two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOF-MS) for metabolomic analysis of polar metabolites / Kimberly Ralston-Hooper ... [et al.] -- LC-MS-based metabolomics / Sunil Bajad and Vladimir Shulaev -- Capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS)-based metabolomics / Philip Britz-McKibbin -- Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics for studies of body fluids and tissues / Heli Nygren ... [et al.] -- Electrospray ionization tandem mass spectrometry (ESI-MS/MS)- based shotgun lipidomics / Giorgis Isaac -- Processing and analysis of GC/LC-MS based metabolomics data / Elizabeth Want and Perrine Masson -- Nuclear magnetic resonance (NMR)-based drug metabolite profiling / Eva M. Lenz -- Nuclear magnetic resonance (NMR)-based metabolomics / Hector C. Keun and Toby J. Athersuch -- Slow magic angle sample spinning : a non- or minimally invasive method for high-resolution ¹H nuclear magnetic resonance (NMR) metabolic profiling / Jian Zhi Hu -- Processing and modeling of nuclear magnetic resonance (NMR) metabolic profiles / Timothy M. D. Ebbels, John C. Lindon, and Muireann Coen.
  • 2015 Springer Protocols
    Bjerrum, Jacob T.
    Metabonomics : analytical techniques and associated chemometrics / Jacob T. Bjerrum -- Sample collection and preparation of biofluids and extracts for NMR spectroscopy / Gwénaëlle Le Gall -- NMR sprectroscopy of biofluids and extracts / Gwénaëlle Le Gall -- High-resolution magic-angle-spinning NMR spectroscopy of intact tissue / Guro F. Giskeødegård, Maria D. Cao, and Tone F. Bathen -- Sample collection and preparation of biofluids and extracts for liquid chromatography-mass spectrometry / Peiyuan Yin [and three others] -- Liquid chromatography-mass spectrometry of biofluids and extracts / Xinjie Zhao [and three others] -- Sample collection and preparation of biofluids and extracts for gas chromatography-mass spectrometry / Abdul-Hamid M. Emwas, Zeyad A. Al-Talla, and Najeh M. Kharbatia -- Gas chromatography-mass spectrometry of biofluids and extracts / Abdul-Hamid M. Emwas [and three others] -- Capillary electrophoresis-mass spectrometry / Masataka Wakayama, Akiyoshi Hirayama, and Tomoyosji Soga -- Preprocessing of raw metabonomic data / Riyas Vettukattil -- Extracting meaningful information from metabonomic data using multivariate statistics / Max Bylesjö -- Combining metabonomics and other -omics data / Mattias Rantalainen -- The strengths and weaknesses of NMR-spectroscopy and mass spectrometry with particular focus on metabolomics research / Abdul-Hamid M. Emwas -- Metabonomics and drug development / Pranov Ramana [and three others] -- Metabonomics and toxicology / Liang Zhao and Thomas Hartung -- Metabonomics and diagnostics / Lucy C. Hicks, Simon J.L. Ralphs, and Horace R.T. Williams -- Metabonomics and systems biology / Vicky De Preter.
  • 2012 Springer
    Kozłowski, Henryk; Linert, W.
    Metal ions in the brain are a necessity as well as a poison. The presence of metal ions in the active sites of biological catalysts or metalloproteins and in the biological functioning of nucleic acids is very well documented and they are required for brain activity. On the other hand, metals are very effective in generating oxidative stress. This effect does not only play a role in immunology but also is the root of practically all neurodegenerative disorders by inducing disease via the death of neurons. Managing metal ions in the brain could therefore be an important strategy in the search for therapeutic agents used in the treatment of neurodegenerative diseases. This new title gives an overview to key topics in the area of metal ions in the brain. It focuses on the role of metal ions in neurological systems by describing their advantageous functions as well as their poisonous features. It is therefore of interest for scientists in biochemistry and biophysics, physiology, toxicology as well as for physicians focused on this topic.
  • ScienceDirect
    Riordan, James F.; Vallee, Bert L.
    Also available: Print – 1991
  • ScienceDirect
    Riordan, James F.; Vallee, Bert L.
    Also available: Print – 1993
  • ScienceDirect
    Riordan, James F.; Vallee, Bert L.
    Also available: Print – 1993
  • 2014 Springer Protocols
    Fontecilla-Camps, Juan C.; Nicolet, Yvain.
    Advances in Research on Metalloproteins -- Purification of O2-Sensitive Metalloproteins -- Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources -- Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins -- Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers -- Electrochemistry of Metalloproteins Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1 -- FTIR Spectroscopy of Metalloproteins -- Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy -- A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds -- Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR) -- Mssbauer Spectroscopy -- X-Ray Absorption Spectroscopy of Metalloproteins -- X-Ray Crystallographic Studies of Metalloproteins -- Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds -- Density Functional Theory Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups.
  • 2011 Springer Protocols
    Stevens, Craig W.
    In silico identification of novel G protein-coupled receptors / Matthew N. Davies, David E. Gloriam, and Darren R. Flower -- Alternative pre-mRNA splicing of G protein-coupled receptors / Ying-Xian Pan, Steven Grinnell, and Gavril W. Pasternak -- Detecting polymorphisms in G protein-coupled receptor genes / Dmitri Proudnikov, Vadim Yuferov, and Mary Jeanne Kreek -- Regulation of the transcription of G protein-coupled receptor genes / Cheol Kyu Hwang [and others] -- Deciphering the evolution of G protein-coupled receptors in vertebrates / Craig W. Stevens -- New approach to visualize endogenously expressed G protein-coupled receptors in tissues and living cells / Gregory Scherrer and Brigitte L. Kieffer -- Posttranslational regulation of G protein-coupled receptors / Yu Qiu and Ping-Yee Law -- Discovering G protein-coupled receptor motifs mediating export from the endoplasmic reticulum / Chunmin Dong and Guangyu Wu -- Identifying G protein-coupled receptor escorts, chaperones, and intracellular tethers regulating receptor density at the cell surface / Stefano Marullo, Liliana Pardo Lopez, and Lamia Achour -- Novel method for determining the kinetics of G protein-coupled receptor plasma membrane expression / Gregory W. Sawyer -- Characterizing the pharmacology of G protein-coupled receptors in transfected cell lines / Kathryn A. Seely and Paul L. Prather -- Novel assay technologies for the discovery of G protein-coupled receptor drugs / Elisa Alvarez-Curto, Richard J. Ward, and Graeme Milligan -- Discovering cell type-specific patterns of G protein-coupled receptor phosphorylation / Kok Choi Kong, Andrew B. Tobin, and Adrian J. Butcher -- Quantifying allosteric modulation of G protein-coupled receptors / Frederick J. Ehlert and Hinako Suga -- Experimental designs for the study of receptor-receptor interactions / Dennis Paul -- Elucidating agonist-selective mechanisms of G protein-coupled receptor desensitization / Chris P. Bailey and Eamonn Kelly -- Detecting the role of arrestins in G protein-coupled receptor regulation / Laura M. Bohn and Patricia H. McDonald -- Characterizing molecular mobility and membrane interactions of G protein-coupled receptors / Vladana Vukojevice, Yu Ming, and Lars Terenius -- Using RNA interference to downregulate G protein-coupled receptors / Philippe Sarret [and others] -- Upregulating G protein-coupled receptors with receptor antagonists / Ellen M. Unterwald.
  • v. 1-, 1955- ScienceDirect
    Abelson, John N.; Colowick, Sidney P.; Kaplan, Nathan Oram; Simon, Melvin I.
  • 2015 Springer Protocols
    Owen, Dylan M.
    Introduction to membrane lipids /Richard M. Epand -- Introduction : membrane properties (good) for life /Marek Cebecauer -- Introduction to fluorescence probing of biological membranes /Alexander P. Demchenko ... [et al.] -- Assembly and use of tethered bilayer lipid membranes (tBLMs) /Charles Cranfield ... [et al.] -- Preparation of detergent-resistant membranes (DRMs) from cultured mammalian cells /Deborah A. Brown -- Isolation of giant plasma membrane vesicles for evaluation of plasma membrane structure and protein partitioning /K. R. Levental and I. Levental -- Asymmetric giant lipid vesicle fabrication /Peichi C. Hu and Noah Malmstadt -- Cholesterol depletion using Methyl-[Beta]-cyclodextrin /Saleemulla Mahammad and Ingela Parmryd -- Comparative LC-MS based profiling approach to analyze lipid composition in tissue culture systems /G. Ekin Atilla-Gokcumen and Ulrike S. Eggert -- Imaging membrane order using environmentally sensitive fluorophores /G.W. Ashdown and Dylan M. Owen -- 3D super-resolution imaging by localization microscopy /Astrid Magenau and Katharina Gaus -- Electron microscopy methods for studying plasma membranes /Alison J. Beckett and Ian A. Prior -- Measuring cytoskeleton and cellular membrane mechanical properties by atomic force microscopy /Charles Roduit ... [et al.] -- Fluorescence linear dichroism imaging for quantifying membrane order /Richard K. P. Benninger -- Scanning fluorescence correlation spectroscopy on biomembranes /Eduard Hermann, Jonas Ries, and Ana J. García-Sáez -- X-ray diffraction of lipid model membranes /Arwen I. I. Tyler, Robert V. Law, and John M. Seddon -- Solid state NMR of lipid model membranes /Arwen I. I. Tyler ... [et al.] -- Fluorescence recovery after photobleaching (FRAP) : acquisition, analysis, and applications /Michael Carnell, Alex Macmillan, and Renee Whan -- Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells /Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton -- Cholesterol behavior in asymmetric lipid bilayers : insights from molecular dynamics simulations /Semen O. Yesylevskyy and Alexander P. Demchenko -- Computer simulations of phase separation in lipid bilayers and monolayers /Svetlana Baoukina and D. Peter Tieleman.
  • 2007 Springer Protocols
    Dopico, Alex M.
  • 2011 ScienceDirect
    Ragsdale, Stephen W.; Rosenzweig, Amy C.
    1. Techniques for cultivating methanogens / Ralph S. Wolfe -- 2. Genetic methods for methanosarcina species / Nicole Buan, Gargi Kulkarni, and William Metcalf -- 3. Genetic systems for hydrogenotrophic methanogens / Felipe Sarmiento B., John A. Leigh, and William B. Whitman -- 4. Molecular tools for investigating ANME community structure and function / Steven J. Hallam ... [et al.] -- 5. Studying gene regulation in methanogenic archaea / Michael Rother, Christian Sattler, and Tilmann Stock -- 6. Growth of methanogens under defined hydrogen conditions / John A. Leigh -- 7. Preparation of [Fe]-hydrogenase from methanogenic archaea / Seigo Shima, Michael Schick, and Haruka Tamura -- 8. Assay of methylotrophic methyltransferases from methanogenic archaea / Donald J. Ferguson, David G. Longstaff, and Joseph A. Krzycki -- 9. Methyl-coenzyme M reductase from methanothermobacter marburgensis / Evert C. Duin, Divya Prakash, and Charlene Brungess -- 10. Methods for analysis of acetyl-CoA synthase : applications to bacterial and archaeal systems / David A. Grahame -- 11. Acetate kinase and phosphotransacetylase / James G. Ferry -- 12. Sodium ion translocation and ATP synthesis in methanogens / Katharina Schlegel and Volker Müller -- 13. Proton translocation in methanogens / Cornelia Welte and Uwe Deppenmeier -- 14. Measuring isotope fractionation by autotrophic microorganisms and enzymes / Kathleen M. Scott, Gordon Fox, and Peter R. Girguis -- 15. 2-oxoacid metabolism in methanogenic CoM and CoB biosynthesis / David E. Graham -- 16. Biomethanation and its potential / Irini Angelidaki ... [et al.].
    Also available: Print – 2011
  • 2011 ScienceDirect
    Ragsdale, Stephen W.; Rosenzweig, Amy C.
    Also available: Print – 2011
  • pt. A-B, 2014. ScienceDirect
    pt. B ScienceDirect
    Macdougald, Ormond A.
  • 2012 Springer
    Jordan, Bertrand.
    Microarray technology has made strong progress over the past decade, and there have also been significant changes in application areas, from nucleic acids to proteomics and from research to clinical applications. This book provides a comprehensive overview of microarrays in diagnostics and biomarker development, covering DNA, peptide, protein and tissue arrays. The focus is on entities that are in actual clinical use, or quite close, and on recent developments, such as peptide and aptamer arrays. A further topic is the miniaturisation towards nanoarrays, which is expected to have great potential in clinical applications. Relevant issues of bioinformatics and statistical analysis of array data are discussed in detail, as well as the barriers to the commercialisation of array-based tests and the vexing IP issues involved. Thus, the book should be very useful tor active array users as well as to newcomers seeking to make the best choice between different technologies.
  • 2013 ScienceDirect
    DeLong, Edward F.
    This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers microbial metagenomics, metatranscriptomics, and metaproteomics, and includes chapters on such topics as in-solution FISH for single cell genome preparation, preparation of BAC libraries from marine microbial community DNA, and preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton. Continues the legacy of this premier serial with quality chapters authored by leaders in the field.
  • 2006 Wiley
    Hecker, M.; Humphery-Smith, Ian.
    "Discover important lessons learned about whole organism biology via microbial proteomics This text provides an exhaustive analysis and presentation of current research in the field of microbial proteomics, with an emphasis on new developments and applications and future directions in research. The editors and authors show how and why the relative simplicity of microbes has made them attractive targets for extensive experimental manipulation in a quest for both improved disease prevention and treatment and an improved understanding of whole organism functional biology. In particular, the text demonstrates how microbial proteomic analyses can aid in drug discovery, including identification of new targets, novel diagnostic markers, and lead optimization. Each chapter is written by one or more leading experts in the field and carefully edited to ensure a consistent and thorough approach throughout. Methods, technologies, and tools associated with the most promising approaches are stressed."--Publisher's website.
    Also available: Print – 2006
  • 2013 Springer Protocols
    Dale, Nicholas; Marinesco, Stephane.
    Microelectrode designs for oxidase-based biosensors / Stephane Marinesco and Olivier Frey -- Microelectrode array fabrication and optimization for selective neurochemical detection / Kevin N. Hascup [and others] -- Advantage of microelectrode technologies for measurement in delicate biological environments such as brain tissue / Andrea Jaquins-Gerstl and Adrian C. Michael -- Hexacyanoferrates as mediators for microelectrode biosensors / Faming Tian and Nicholas Dale -- Enzyme immobilization on microelectrode biosensors / Natalia Vasylieva and Stephane Marinesco -- Characterization of polymer-enzyme composite biosensors for brain monitoring in vivo / Robert D. O'Neill -- Monitoring extracellular glutamate in the brain by microdialysis and microsensors / Si Qin [and others] -- Sub-second measurements of glutamate and other neurotransmitter signaling using enzyme-based ceramic microelectrode arrays / Erin R. Hascup [and others] -- Regulation of extracellular concentrations of D-serine in the central nervous system revealed by D-amino acid oxidase microelectrode biosensors / Pierre Pernot, Caroline Maucler, and Stephane Marinesco -- Measurement of purine release with microelectrode biosensors / Nicholas Dale -- Glucose and lactate monitoring across the rat sleep-wake cycle / Raymond Cespuglio, Larissa Netchiporouk, and Nataliya Shram -- Forebrain cholinergic systems and cognition : new insights based on rapid detection of choline spikes using enzyme-based biosensors / Vinay Parikh and Martin Sarter -- Carbon nanotubes-based microelectrode (bio)sensors / Paloma Yanez-Sedeno, Lourdes Agui, and Jose M. Pingarron -- Label-free affinity biosensors based on electrochemical impedance spectroscopy / Nicole Jaffrezic-Renault -- Aptamer-based electrochemical biosensors for the detection of small molecules and plasma proteins / Cassie Ho and Hua-Zhong Yu -- Electrochemiluminescent biosensors : neuroscience applications / Robert J. Forster and Tia E. Keyes -- Molecularly imprinted polymers : promising advanced materials for in vivo sensing / Yi Ge [and others].
  • 2010 Springer
    Wang, Zhiguo; Yang, Baofeng.
  • 2009. Springer
    Wang, Zhiguo.
  • 2007 ScienceDirect
    Hannon, Gregory J.; Rossi, John J.
    Also available: Print – 2007
  • 2007 Springer Protocols
    Zhou, Jun.
  • 2010 Springer
    Bienert, Gerd P.; Jahn, Thomas P.
    Aquaporins : a family of highly regulated multifunctional channels / Charles Hachez and François Chaumont -- Phylogeny of major intrinsic proteins / Jonas Å.H. Danielson and Urban Johanson -- Metalloids, soil chemistry, and the environment / Enzo Lombi and Peter E. Holm -- Arsenic transport in prokaryotes and eukaryotic microbes / Barry P. Rosen and Markus J. Tamøs -- Metalloid transport by aquaglyceroporins : consequences in the treatment of human diseases / Rita Mukhopadhyay and Eric Beitz -- Roles of vertebrate aquaglyceroporins in arsenic transport and detoxification / Zijuan Liu -- Molecular mechanisms of boron transport in plants : involvement of arabidopsis NIP5;1 and NIP6;1 / Kyoko Miwa ... [et al.] -- Silicon transporters in higher plants / Jian Feng Ma -- Major intrinsic proteins and arsenic transport in plants : new players and their potential roles / Gerd P. Bienert and Thomas P. Jahn -- Major intrinsic proteins in biomimetic membranes / Claus Hølix Nielsen.
    Also available: Print – 2010
  • 2006 Springer
    Murphy, Regina M.; Tsai, Amos M.
  • 2007 Springer
    Schaffer, Stephen W.; Suleiman, M.-Saadeh.
    Regulation of mitochondrial respiration in heart muscle / Ilmo Hassinen -- Regulation of fatty acid oxidation of the heart / Clifford D.L. Folmes and Gary D. Lopaschuk -- Regulation of mitochondrial fuel handling by the peroxisome proliferator-activated receptors / Mary C. Sugden and Mark J. Holness -- Molecular structure of the mitochondrial citrate transport protein / Ronald S. Kaplan and June A. Mayor -- Regulation of pyruvate and amino acid metabolism / Thomas C. Vary, Wiley W. Souba, and Cristopher J. Lynch -- Amino acids and the mitochondria / Nicola King -- Mechanotransduction of shear-stress at the mitochondria / Abu-Bakr Al-Mehdi -- Formation of reactive oxygen species in mitochondria / Julio F. Turrens -- Mitochondrial calcium : role in the normal and ischaemic/reperfused myocardium / Elinor J. Griffiths ... [et al.] -- Mitochondrial ion channels / Brian O'Rourke -- The mitochondrial permeability transition pore : from molecular mechanism to reperfusion injury and cadioprotection / Andrew P. Halestrap, Samantha J. Clarke, and Igor Khalilin -- The apoptotic mitochondrial pathway : modulators, interventions, and clinical implications / M.-Saadeh Suleiman and Stephen W. Schaffer -- The role of mitochondria in necrosis following myocardial ischemia-reperfusion / Elizabeth Murphy and Charles Steenbergen -- Mitochondria and their role in ischemia/reperfusion injury / Sebastian Phillip, James M. Downey, and Michael V. Cohen -- Mitochondrial DNA damage and repair / Inna N. Shokolenko, Susan P. Ledoux, and Glenn L. Wilson.
  • 2005 CRCnetBASE
    Berdanier, Carolyn D.
    Introduction to mitochondria / Carolyn D. Berdanier -- Mitochondrial bioenergetics in intact tissue / Richard L. Veech -- The human mitochondrial genome: mechanisms of expression and maintenance / Jan-Willem Taanman and Sion Llewellyn Williams -- Oxidative phosphorylation disease: diagnosis and pathogenesis / John M. Shoffner -- Using mitochondrial DNA in population surveys / Carolyn D. Berdanier -- Mitochondrial DNA mutation, oxidative stress, and alteration of gene expression in human aging / Hsin--Chen Lee and Yau-Huei Wei -- Mitochondrial DNA in cardiomyopathies / Nobuakira Takeda -- Mitochondria in Diabetes mellitus / Hong Kyu Lee -- Drugs, nutrients, and hormones in mitochondrial function / Carolyn D. Berdanier -- Bacterial FF ATP synthase as a model for complex V / Brian D. Cain, Tammy Bohannon Grabar, and Depa Bhatt -- Cybrids in the study of animal mitochondrial genetics and pathology / Ian A. Trounce and Carl A. Pinkert -- Animal modeling: from transgenesis to transmitochondrial models / Carl A. Pinkert and Ian A. Trounce -- BHE/Cdb rats as tools to study mitochondrial gene expression / Carolyn D. Berdanier and Moon-Jeong Chang Kim -- Appendix 1 -- Appendix 2.
  • 2013 Springer
    Wong, Lee-Jun C.
    Mitochondrial cytopathies are mutations in the inherited maternal mitochondrial genome, or the nuclear DNA-mutation. Mitochondrial respiratory chain disorders (RCD) are a group of genetically and clinically heterogeneous diseases, due to the fact that protein components of the respiratory chain are encoded by both mitochondrial and nuclear genomes and are essential in all cells. In addition, the biogenesis, structure and function of mitochondria, including DNA replication, transcription, and translation, all require nuclear encoded genes. Since mitochondria are present in every cell, every tissue, mitochondrial disorders usually affects multiple organs. Mitochondrial Disorders Caused by Nuclear Genes discusses the biochemical, molecular, clinical, and genetic aspects of complex dual genome mitochondrial disorders. Chapters include genes involved in mitochondrial DNA biogenesis and maintenance of mitochondrial DNA integrity, complex subunits and assembly genes, and mitochondrial protein translation related diseases.
  • 2014 ScienceDirect
    Chan, David C.; Murphy, Anne N.
  • 2009 ScienceDirect
    Allison, William S.; Scheffler, Immo E.
    Also available: Print – 2009
  • 2009 ScienceDirect
    Allison, William S.; Murphy, Anne N.
    Also available: Print – 2009
  • 2012 Springer
    Kadenbach, Bernhard.
    Introduction to Mitochondrial Oxidative Phosphorylation / Bernhard Kadenbach -- Mitochondrial Dynamics: The Intersection of Form and Function / Andrew Ferree and Orian Shirihai -- Biogenesis of Mitochondrial Proteins / Johannes M. Herrmann, Sebastian Longen, Daniel Weckbecker and Matthieu Depuydt -- Assembly Factors of Human Mitochondrial Respiratory Chain Complexes: Physiology and Pathophysiology / Daniele Ghezzi and Massimo Zeviani -- Supramolecular Organisation of the Mitochondrial Respiratory Chain: A New Challenge for the Mechanism and Control of Oxidative Phosphorylation / Giorgio Lenaz and Maria Luisa Genova -- Molecular Mechanisms of Superoxide Production by the Mitochondrial Respiratory Chain / Stefan Dröse and Ulrich Brandt -- Studies on the Function and Regulation of Mitochondrial Uncoupling Proteins / Richard K. Porter -- Evolution of the Couple Cytochrome c and Cytochrome c Oxidase in Primates / Denis Pierron, Derek E. Wildman, Maik Hüttemann, Thierry Letellier and Lawrence I. Grossman -- Reaction Mechanism of Mammalian Mitochondrial Cytochrome c Oxidase / Shinya Yoshikawa, Kazumasa Muramoto and Kyoko Shinzawa-Itoh -- Phosphorylation of Mammalian Cytochrome c and Cytochrome c Oxidase in the Regulation of Cell Destiny: Respiration, Apoptosis, and Human Disease / Maik Hüttemann, Icksoo Lee, Lawrence I. Grossman, Jeffrey W. Doan and Thomas H. Sanderson -- Individual Biochemical Behaviour Versus Biological Robustness: Spotlight on the Regulation of Cytochrome c Oxidase / Rabia Ramzan, Petra Weber, Bernhard Kadenbach and Sebastian Vogt -- Bigenomic Regulation of Cytochrome c Oxidase in Neurons and the Tight Coupling Between Neuronal Activity and Energy Metabolism / Margaret T. T. Wong-Riley -- Cytochrome c Oxidase and Its Role in Neurodegeneration and Neuroprotection / Susanne Arnold -- The Many Clinical Faces of Cytochrome c Oxidase Deficiency / Salvatore DiMauro, Kurenai Tanji and Eric A. Schon.
  • 2013 Springer
    Strasser, Andrea; Wittmann, Hans-Joachim.
    This practical introduction to molecular modelling of GPCRs covers core principles, and moves on to present advanced GPCR modelling techniques. Includes data on crystal structures and essential amino acid sequences, as well as LINUX commands and examples.
  • 2010
    Ghosh, Samantak; Chen, James K.; Huestis, Wray H.; Kool, Eric T.
    This thesis discusses a number of projects involving the use of modified nucleotides and oligonucleotides in addressing some basic science questions and some clinical and technological applications.The first chapter details our efforts at using telomere-encoding circular DNA in elongating zebrafish telomeres. We microinjected our synthetic circular DNA into zebrafish embryos and studied their telomere length 24 hrs later. Using Quantitative Fluorescence in situ Hybridization (Q-FISH) as the analytical tool to determine telomere length, we observed no significant difference in telomere length between the group injected with the synthetic DNA and the control group. In the second chapter we studied the potential of a non-polar shape mimic of iodo-uracil as an imager of tumors. We continued discussing our work with non-polar nucleotide isosteres in the third chapter where we used them in investigating a novel active site in polymerase from Pyrococcus furiosus (Pfu). From our studies we concluded that although shape was an important factor in distinguishing bases, this binding site also employed hydrogen bonding to identify nucleoabases. The lack of any recognition of the syn-oxidized bases suggested that the enzyme preferred to recognize bases in the anti conformation rather than syn. In the fourth chapter we were interested in understanding the factors determining the fidelity and selectivity observed in RNA Polymerase II mediated transcription. Once again we used non-polar shape mimics of thymidine (dF) and adenine (dQ) to study the importance of shape and hydrogen bonding. We observed that the thymidine mimic was recognized better by the RNA Polymerase II active site than the mis-match bases. The adenine isostere on the other hand, was poorly recognized. This preliminary study demonstrates the importance of both shape and hydrogen bonding. The last chapter discusses our studies using polyfluorophores on a DNA backbone to detect gases. Following a combinatorial method, a library of oligodeoxyfluorosides (ODFs) were synthesized from which sensors of gases were selected. Using this method we were able to select optical sensors for a diverse set of small molecules in vapor state.
  • 2009 Springer
    Rubin, Eric; Sakamoto, Kathleen.
  • 2011
    Brown, Frank Curtis; Harbury, Pehr A. B.; Khosla, Chaitan; Pfeffer, Suzanne R.
    Mannose 6-phosphate receptors (MPRs) deliver lysosomal acid hydrolases to the endosomal pathway and are then returned from late endosomes to the trans-Golgi network (TGN) for another round of transport. The long coiled-coil protein GCC185 tethers MPR-containing vesicles at the TGN. In the second chapter of this thesis, recent progress in the study of tethers in other laboratories is reviewed. The third chapter describes a mechanism by which GCC185 may tether MPR-containing vesicles. Distinct domains required for the two known functions of this protein, maintenance of Golgi structure and tethering MPR transport vesicles, were identified. The domain needed for vesicle tethering was shown to bind the clathrin adaptor AP-1. Moreover, data are presented showing the presence of AP-1 on transport vesicles en route to the Golgi complex. These data suggest that GCC185, which is localized to the TGN through Arl1- and Rab6-GTPase binding interactions at its C-terminus, engages incoming transport vesicles by binding AP-1 via an internal coiled-coil domain near central, putative "hinge" regions of the protein. Work presented in the fourth chapter describes progress toward understanding the Golgi fragmentation phenotype of cells depleted of GCC185. In addition to the C-terminal localization domain, an N-terminal domain required for Golgi structure maintenance was identified. In addition, GCC185 binding sites for numerous Golgi-localized Rab GTPases were identified, suggesting a model in which GCC185 can make lateral connections across the Golgi ribbon in order to maintain Golgi structure or promote a fused state in the equilibrium between membrane fusion and fission characteristic of the Golgi complex.
  • 2010 Springer
    Yon-Kahn, Jeannine; Hervé, Guy.
  • 2011
    Peterson, Soren Joseph; Brown, Patrick O.; Krasnow, Mark; Luo, Liqun; Straight, Aaron.
    From a structural standpoint, one of the most characteristic general design elements of the mammalian organism is its tubular nature. The lung and circulatory system shuttle oxygen and nutrients to target tissues and allow for the excretion of waste products. In some ways, the vasculature lies at the center of human physiology--its passageways provide the infrastructure for maintaining homeostasis. Despite the importance of tubular networks in human health and disease, we have a poor understanding of many aspects of the genetic, molecular, and cellular programs controlling the development of these complex structures. The Drosophila melanogaster tracheal system, an elaborate network of hollow epithelial tubes, transports gases to and from target tissues. The tracheal system, with its simple structure, tractable genetics, and substantial experimental toolkit has emerged as an excellent model system for studying questions with relevance to more complex tubular systems. During development, tracheal branches ramify on the surface of target tissues, providing oxygen to every cell in the body. However, in a phenomenon unique to the Drosophila flight muscle, trachea are also present within plasma membrane invaginations deep below the muscle's outer extremities and have been described to surround every mitochondria of the flight muscle, thus coupling oxygen delivery directly to aerobic respiration at the mitochondrion. Although the presence of trachea within flight muscle membrane invaginations has been described for over 150 years, the developmental progression and cellular and molecular basis of this subcellular targeting process is unknown. In Chapter 2, we show that tracheal branches invade the developing flight muscle Transverse (T)-tubule plasma membrane invagination system during a brief period of pupal development. Branchless (Bnl) FGF, a fibroblast growth factor that functions as a chemoattractant, is required in the flight muscle, and its cognate receptor, Breathless (Btl) FGFR, is required in trachea for the tracheal invasion process to occur. Whereas Bnl FGF is localized to all flight muscle plasma membranes prior to tracheal invasion, during invasion Bnl FGF localizes preferentially to the T-tubule and is excluded from the surrounding plasma membrane. In addition to Bnl FGF, core polarity regulators commonly found on basolateral membranes in epithelial cells also preferentially localize to the T-tubule network during tracheal invasion. We find that depletion of AP-1[gamma], targeting machinery required for basolateral secretion in Drosophila epithelia, can also reroute Bnl FGF secretion to the outer plasma membrane and away from T-tubule openings, shifting trachea to the plasma membrane and away from T-tubules. We propose that (1) polarized secretion of Bnl FGF to the T-tubule guides tracheal branches into the T-tubule network and that (2) polarized Bnl FGF secretion is established via the redeployment of ancestral basolateral secretion pathways to the T-tubules, a membrane domain having molecular signatures of epithelial basolateral domains. To our knowledge, compartmentalized secretion of Bnl FGF to flight muscle T-tubule membranes is the first example of polarized subcellular secretion of a growth factor with functional consequences for the development of another tissue. In Chapter 3, we examine the molecular machinery involved in maintaining the polarized secretion of Bnl FGF during tracheal invasion. We find a host of secretory machinery, including several Rabs, Myosin V, an actin nucleator, and others, to be involved in the secretion of Bnl-FGF to the T-tubule during tracheal invasion. From these data we propose a molecular model to explain polarized Bnl FGF secretion at the T-tubule. Why is the flight muscle the only tissue invaded by trachea? In Chapter 4, we find that the flight muscle is structurally adapted to allow its T-tubule plasma membrane invagination network to be co-opted by migrating tracheal processes. In contrast to other muscles, the flight muscle T-tubule network forms large holes on the muscle surface as part of its development. The tracheal invasion process in the flight muscle is therefore the consequence not only of a polarized secretion process as discussed in Chapter 2, but also a structurally distinct stage of flight muscle development. In the final chapter, we search for the fine tracheal tubes reported to target and form contacts on the flight muscle mitochondria. We find an extracellular protein-based lattice of the appropriate diameter and localization to potentially represent the reported tracheal extensions to the mitochondria and propose several models of protein lattice formation. We hope that insight derived from this work spurs future investigators in a host of areas. The exquisite example of targeting to specific membrane domains dependent on a subcellular chemoattractant gradient demonstrated by the invading tracheal branch may provide insight into a number of developmental scenarios where fine targeting of different cells is required. We also hope that our finding regarding polarized secretion in muscle inspires new ways to think about the assembly of muscle membranes during development and disease states.
  • 2003 CRCnetBASE
    Sanders, Tom; Emery, Peter.
  • v. 1-2, 1985-86.
    Feuer, George; De la Iglesia, Felix A.
    Status: Not Checked OutLane Catalog Record
  • 2013 Springer
    Coller, Jeff; Khalil, Ahmad M.
    Chromatin regulation by long non-coding RNAs / Daniel C. Factor, Paul J. Tesar and Ahmad M. Khalil -- Regulatikon of eukaryotic cell differentiation by long non-coding RNAs / Juan R. Alvarez-Dominguez, Wenqian Hu and Harvey F. Lodish -- Roles of long non-coding RNAs in X-chromsome inactivation / J. Mauro Calabrese and Terry Magnuson -- Roles of long non-coding RNAs in genomic imprinting / Kristen Martins-Taylor and Stormy J. Chamberlain -- Dysregulation of long non-coding RNAs in human disease / Nianwei Lin and Tariq M. Rana -- Functions of long non-coding RNAs in non-mamallian systems / Alex Tuck and David Tollervey -- Emerging technologies to study long non-coding RNAs / Fereshteh Jahaniani ... [et al.]
  • 2006 Springer
    Martinoia, Enrico; Tamás, Markus J.
  • 2006 Springer
    Gaestel, Matthias.
  • 2014 Springer
    Burtness, Barbara; Golemis, Erica.
    Head and neck cancers, involving sites from the nasopharynx to the subglottic larynx, are frequently devastating cancers that afflict patients around the world. These cancers are frequently locally advanced prior to detection, and require multimodality therapy that is associated with high morbidity. As this book addresses this difficult disease, it accomplishes three main goals. First, it introduces the etiology and subclasses of squamous cell carcinomas of the head and neck (SCCHNs), and how these factors affect prognosis. Although habitual exposures to tobacco, alcohol, and other agents have historically been the main causes of SCCHN, a rising proportion of oropharynx cancers arise from transforming human papillomavirus (HPV) infection. These two broad classes of SCCHN have significant differences in disease profile and response to treatment, as we discuss. Second, it summarizes the current state of understanding of the genetic, epigenetic and protein expression changes associated with the various classes of SCCHN. In the past decade, disease pathogenesis of SCCHN has been appreciated to involve deregulation of multiple tumor pathways, including the receptor tyrosine kinases (RTKs) EGFR, ERBB2, ERBB3, c-MET, and IGF1R; transforming growth factor β (TGFβ); Notch; cytoplasmic signaling proteins including PTEN, PI3K, JAK/STAT, and Wnt-responsive β-catenin; mutation control systems, including p53 and the DNA damage repair (DDR) machinery; and hypoxic response. The specific understanding of the action of these proteins in SCCHN is presented here. Finally, this book defines potential therapeutic targets for improved management of the disease in the future, discussing prospects for improved prediction of prognosis.
  • 2008 Springer Protocols
    Mason, Ivor; Sharpe, Paul T.
    The mouse as a developmental model / Paul T. Sharpe -- Culture of postimplantation mouse embryos / Paul Martin and David L. Cockroft -- Organ culture in the analysis of tissue interactions / Irma Thesleff and Carin Sahlberg -- Treatment of mice with retinoids in vivo and in vitro / Gillian M. Morriss-Kay -- Analysis of skeletal ontogenesis through differential staining of bone and cartilage / Michael J. Depew -- Cell grafting and labeling in postimplantation mouse embryos / Gabriel A. Quinlan ... [et al.] -- Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-celled eggs / David Murphy -- Cre recombinase mediated alterations of the mouse genome using embryonic stem cells / Anna-Katerina Hadjantonakis, Melinda Pirity, and András Nagy -- Gene trapping in mouse embryonic stem cells / Jane Brennan and William C. Skarnes -- Application of lacZ transgenic mice to cell lineage studies / Catherine M. Watson ... [et al.] -- Transgenic RNA interference to investigate gene function in the mouse / Tilo Kunath -- Mouse primordial germ cells : isolation and in vitro culture / Patricia A. Labosky and Brigid L.M. Hogan -- Gene transfer to the rodent embryo by retroviral vectors / Marla B. Luskin -- The avian embryo : an overview / Ivor Mason -- Chick embryos : incubation and isolation / Ivor Mason -- New culture / Amata Hornbruch -- EC culture : a method to culture early chick embryos / Andrea Streit -- Grafting Henson's node / Claudio D. Stern -- Microsurgical manipulation of the notochord / Lúcia E. Alvares ... [et al.] -- Transplantation of avian neural tissue / Sarah Guthrie -- Grafting of apical ridge and polarizing region / Cheryl Tickle -- Tissue recombinations in collagen gels / Marysia Placzek -- Quail-chick chimeras / Marie-Aimée Teillet, Catherine Ziller, and Nicole M. Le Douarin -- Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo / Jonathan D.W. Clarke -- Gene transfer in avian embryos using replication-competent retroviruses / Cairine Logan and Philippa Francis-West -- Electroporation in avian embryos / Jun-ichi Funahashi and Harukazu Nakamura -- An overview of Xenopus development / Michael Jones and James C. Smith -- Mesoderm induction assays / C. Michael Jones and James C. Smith -- Experimental embryological methods for analysis of neural induction in the amphibian / Ray Keller, Ann Poznanski, and Tamira Elul -- A method for generating transgenic frog embryos / Shoko Ishibashi, Kristin L. Kroll, and Enrique Amaya -- Axolotl/newt / Malcolm Maden -- The zebrafish : an overview of its early development / Nigel Holder and Qiling Xu -- Small-scale marker-based screening for mutations in zebrafish development / Peter D. Currie, Thomas F. Schilling, and Philip W. ingham -- Microinjection and cell transplantation in zebrafish embryos / Qiling Xu, Derek Stemple, and Katherine Joubin -- Recent advances in meganuclease- and transposon-mediated transgenesis of medaka and zebrafish / Clemens Grabher and Joachim Wittbrodt -- Retinoids in nonmammalian embryos / Malcolm Maden -- Protochordates / Peter W.H. Holland and Hiroshi Wada -- Subtractive hybridization and construction of cDNA libraries / Bruce Blumberg and Juan Carlos Izpisúa Belmonte -- Differential display of eukaryotic mRNA / Antonio Tugores and Juan Carlos Izpisúa Belmonte -- Using DNA microarrays / Clare Pritchard, Peter Underhill, and Andy Greenfield -- Profiling gene transcription in the developing embryo : microarray analysis on gene chips / David Chambers and Andrew Lumsden -- RT-PCR on embryos using degenerate oligonucleotide primers / Anthony Graham -- Single-cell RT-PCR cDNA subtraction / Ebrahim Sakhinia ... [et al.] -- In-situ hybridization of rdioactive riboprobes to RNA in tissue sections / Radma Mahmood and Ivor Mason -- In-situ hybridization and immunohistochemistry in whole embryos / Carol Irving -- Wholemount in situ hybridization to Xenopus embryos / C. Michael Jones and James C. Smith -- Wholemount in situ hybridization to Amphioxus embryos / Peter W.H. Holland -- In-situ hybridization to sections (nonradioactive) / Maria Rex and Paul J. Scotting -- Immunohistochemistry using polyester wax / Andrew Kent -- Immunohistochemistry on whole embryos / Ivor Mason -- Whole embryo assays for programmed cell death / Anthony Graham -- Protein techniques : immunoprecipitation, in vitro kinase assays, and western blotting / Clive Dickson -- Microscopy and photmicrography techniques / Richard J.T. Wingate.
  • 2008 Springer
    Burleigh, Barbara A.; Soldati, Dominique.
    Current and emerging approaches to studying invasion in apicomplexan parasites / Jeffrey Mital and Gary E. Ward -- Microneme proteins in apicomplexans / Vern B. Carruthers and Fiona M. Tomley -- Erythrocyte invasion by plasmodium falciparum: multiple ligand-receptor interactions and phenotypic switching / Manoj T. Duraisingh ... [et al.] -- Role of the gp85/trans-sialidase superfamily of glycoproteins in the interaction of trypanosoma cruzi with host structures / Maria Jølia M. Alves and Walter Colli -- Calcium regulation and signaling in apicomplexan parasites / Kisaburo Nagamune ... [et al.] -- Trypanosoma cruzi: parasite and host cell signaling during the invasion process / Nobuko Yoshida and Mauro Cortez -- Host cell actin remodeling in response to cryptosporidium / Steven P. O'Hara ... [et al.] -- Host cell actin remodeling in response to trypanosoma cruzi: trypomastigote versus amastigote entry / Renato A. Mortara ... [et al.] -- Actin/myosin-based gliding motility in apicomplexan parasites / Kai Matuschewski and Herwig Schøler -- Roles of proteases during invasion and egress by plasmodium and toxoplasma / Timothy J. Dowse ... [et al.] -- Roles of naturally occurring protease inhibitors in the modulation of host cell signaling and cellular invasion by trypanosoma cruzi / Julio Scharfstein and Ana Paula C.A. Lima -- Biogenesis of and activities at the toxoplasma gondii parasitophorous vacuole membrane / Anthony P. Sinai. The role of host cell lysosomes in trypanosoma cruzi invasion / G. Adam Mott and Barbara A. Burleigh -- Leishmania invasion and phagosome biogenesis / Robert Lodge and Albert Descoteaux -- Plasmodium sporozoite passage across the sinusoidal cell layer / Ute Frevert ... [et al.] -- Transepithelial migration by toxoplasma / Antonio Barragan and Niclas Hitziger -- The microsporidian polar tube and its role in invasion / Frederic Delbac and Valerie Polonais -- Intestinal invasion by entamoeba histolytica / Shahram Solaymani-Mohammadi and William A. Petri, Jr.
  • 2015 Springer Protocols
    Kukol, Andreas.
    Molecular dynamics simulations / Erik R. Lindahl -- Monte Carlo simulations / David J. Earl and Michael W. Deem -- Hybrid quantum and classical methods for computing kinetic isotope effects of chemical reactions in solutions and in enzymes / Jiali Gao ... [et al.] -- Comparison of protein force fields for molecular dynamics simulations / Olgun Guvench and Alexander D. MacKerell, Jr. -- Normal modes and essential dynamics / Steven Hayward and Bert L. de Groot -- Calculation of absolute protein-ligand binding constants with the molecular dynamics free energy perturbation method / Hyung-June Woo -- Free energy calculations applied to membrane proteins / Christophe Chipot -- Molecular dynamics simulations of membrane proteins / Philip C. Biggin and Peter J. Bond -- Membrane-associated proteins and peptides / Marc F. Lensink -- Implicit membrane models for membrane protein simulation / Michael Feig -- Comparative modeling of proteins / Gerald H. Lushington -- Transmembrane protein models based on high-throughput molecular dynamics simulations with experimental constratints / Andrew J. Beevers and Andreas Kukol -- Nuclear magnetic resonance-based modeling and refinement of protein three-dimensional structures and their complexes / Gloria Fuentes, Aalt D.J. van Dijk, and Alexandre M.J.J. Bonvin -- Conformational changes in protein function / Haiguang Liu ... [et al.] -- Protein folding and unfolding by all-atom molecular dynamics simulations / Hongxin Lei and Yong Duan -- Modeling of protein misfolding in disease / Edyta B. Małolepsza -- Identifying putative drug targets and potential drug leads : starting points for virtual screening and docking / David S. Wishart -- Receptor flexibility for large-scale in silico ligand screens : chances and challenges / B. Fischer, H. Merlitz, and W. Wenzel -- Molecular docking / Garrett M. Morris and Marguerita Lim-Wilby.
  • 2008 Springer Protocols
    Kukol, Andreas.
    Molecular dynamics simulations / Erik R. Lindahl -- Monte Carlo simulations / David J. Earl and Michael W. Deem -- Hybrid quantum and classical methods for computing kinetic isotope effects of chemical reactions in solutions and in enzymes / Jiali Gao ... [et al.] -- Comparison of protein force fields for molecular dynamics simulations / Olgun Guvench and Alexander D. MacKerell, Jr. -- Normal modes and essential dynamics / Steven Hayward and Bert L. de Groot -- Calculation of absolute protein-ligand binding constants with the molecular dynamics free energy perturbation method / Hyung-June Woo -- Free energy calculations applied to membrane proteins / Christophe Chipot -- Molecular dynamics simulations of membrane proteins / Philip C. Biggin and Peter J. Bond -- Membrane-associated proteins and peptides / Marc F. Lensink -- Implicit membrane models for membrane protein simulation / Michael Feig -- Comparative modeling of proteins / Gerald H. Lushington -- Transmembrane protein models based on high-throughput molecular dynamics simulations with experimental constraints / Andrew J. Beevers and Andreas Kukol -- Nuclear magnetic resonance-based modeling and refinement of protein three-dimensional structures and their complexes / Gloria Fuentes, Aalt D.J. van Dijk, and Alexandre M.J.J. Bonvin -- Conformational changes in protein function / Haiguang Liu ... [et al.] -- Protein folding and unfolding by all-atom molecular dynamics simulations / Hongxin Lei and Yong Duan -- Modeling of protein misfolding in disease / Edyta B. Małolepsza -- Identifying putative drug targets and potential drug leads : starting points for virtual screening and docking / David S. Wishart -- Receptor flexibility for large-scale in silico ligand screens : chances and challenges / B. Fischer, H. Merlitz, and W. Wenzel -- Molecular docking / Garrett M. Morris and Marguerita Lim-Wilby.
  • 2011
    Egeler, Emily Lawson; Ferrell, James Ellsworth; Kopito, Ron; Wandless, Thomas.
    Cellular maintenance of protein homeostasis , or proper protein folding, is essential for normal cellular function. Accumulation of misfolded protein can lead to disease and even cell death. The ubiquitin-proteasome system plays a central role in processing cellular proteins for degradation, but little is currently known about how misfolded cytosolic proteins are recognized by protein quality control machinery and targeted for degradation in mammalian cells. In the work presented here, we work with a model protein substrate that is unstable when expressed in cells, but stabilized by addition of a small cell-permeable ligand. Using purified protein, we show that unstable mutants are poorly folded or rapidly sampling unfolded states. The propensity to unfold in vitro correlates with observed instability when expressed in cells. We also show that these substrates are rapidly ubiquitinated en route to degradation at the proteasome in a ligand-dependent manner. We conducted a mass spectrometry screen to identify endogenous protein interactors with the model substrate, which identified a few annotated ubiquitin-proteasome system components. The findings presented herein correlate observed cellular instability to both the propensity of the protein to unfold and the extent of ubiquitination in the ligand-free state. We propose that this protein is a ligand-dependent substrate for the protein quality control machinery, providing an excellent substrate into further query the mechanisms of protein quality control.
  • 2015 Springer
    Bandiera, Stelvio M.; Hrycay, Eugene G.
    Foreword -- 1. Monooxygenase, Peroxidase and Peroxygenase Properties and Reaction Mechanisms of Cytochrome P450 Enzymes -- 2. Oxidizing Intermediates in P450 Catalysis: A Case for Multiple Oxidants -- 3. Current Approaches for Investigating and Predicting Cytochrome P450 3A4-Ligand Interactions -- 4. Acyl-Carbon Bond Cleaving Cytochrome P450 Enzymes: CYP17A1, CYP19A1 and CYP51A1 -- 5. Regioselective Versatility of Monooxygenase Reactions Catalyzed by CYP2B6 and CYP3A4: Examples With Single Substrates -- 6. Cytochrome P450 Enzymes in the Bioactivation of Polyunsaturated Fatty Acids and their Role in Cardiovascular Disease -- 7. Monooxygenation of Small Hydrocarbons Catalyzed by Bacterial Cytochrome P450s -- 8. Use of Chemical Auxiliaries to Control P450 Enzymes for Predictable Oxidations at Unactivated C-H Bonds of Substrates,- 9. Cytochrome P450 Enzymes and Electrochemistry: Crosstalk with Electrodes as Redox Partners and Electron Sources -- 10. Mechanistic Basis of Electron Transfer to Cytochromes P450 by Natural Redox Partners and Artificial Donor Constructs -- 11. Biological Diversity of Cytochrome P450 Redox Partner Proteins -- 12. Cytochrome P450cin (CYP176A1) -- 13. Fungal Unspecific Peroxygenases: Heme-Thiolate Proteins that Combine Peroxidase and Cytochrome P450 Properties.
    Also available: Print – 2015
  • 2012 Springer
    Kaul, Sunil; Wadhwa, Renu.
    Part 1. Structure and Function of Mortalin / Birth of Mortalin: Multiple Names, Niches and Functions Connecting Stress, Senescence and Cancer / Renu Wadhwa and Sunil C. Kaul -- Mortalin's Machinery / Custer C. Deocaris, Sunil C. Kaul and Renu Wadhwa -- The Role of Mortalin in Iron Homeostasis / Wen-I Luo and James A. Cowan -- Functional Characteristics of Mortalin / Walter A. Baseler, Tara L. Croston and John M. Hollander -- Part 2. Mortalin in Evolution2 Deocaris / Mortalin and Drosophila DmHsp22: Two Mitochondrial Chaperones Regulating Aging and Carcinogenesis / Marie Le Pécheur, Geneviève Morrow and Robert M. Tanguay -- Mortalin in Invertebrates and The Induction of Apoptosis by Wild-Type p53 Following Defeat of Mortalin-Based Cytoplasmic Sequestration in Cancerous Clam Hemocytes / Charles W. Walker, Ben Low and S. Anne Böttger -- Mortalin and Stem Cells: A Study from Planarians / Renata Batistoni -- Part 3. Mortalin in Health and Disease / Mortalin in Cell Protection from Immune Attack / Moran Saar, Oren Moskovich and Zvi Fishelson -- Mortalin in Neurological Diseases / Jinghua Jin, Jing Zhang, Travis J. Cook and Jake G. Hoekstra -- Loss of Mortalin Function in Parkinson's Disease-Supporting the Mitochondrial Pathway of Neurodegeneration / Lena F. Burbulla and Rejko Krüger -- Hsp75/mortalin and Protection from Ischemic Brain Injury / Robin E. White, Yi-Bing Ouyang and Rona G. Giffard -- Catecholamine Regulated Protein (CRP40), A Splice Variant of Mortalin-2: Functional Role in CNS Disorders / Joseph P. Gabriele, Sarah E. Groleau, Ritesh P. Daya, Zdenek B. Pristupa and Ram K. Mishra -- Chaperonopathies: Diseases in Which Mortalin and Other Hsp-Chaperones Play a Role in Etiology and Pathogenesis / Alberto J. L. Macario, Francesco Cappello and Everly Conway de Macario -- Part 4. Mortalin and Cancer / Many Faces of Mortalin and Tid1 / Ohad Iosefson and Abdussalam Azem -- Mortalin: A Positive Regulator of Centrosome Duplication and Amplification / Masayuki Kanai and Kenji Fukasawa -- Mortalin Expression in Normal and Neoplastic Tissues / Angheliki Nomikos, Sinclair R. Dundas and Graeme I. Murray -- Mortalin-p53 Interaction as a Target for Liver Cancer Therapy / Wen-Jing Lu, Nikki P. Lee, Renu Wadhwa and John M. Luk -- Mortalin Targeting Gadgets for Cancer Therapy / Chae-Ok Yun and Renu Wadhwa -- Part 5. Mortalin Based Technologies / Cell Internalizing Anti-Mortalin Antibody for Generation of Illuminating MSCs for Long-Term In vitro and In vivo Tracking / Toshimasa Uemura, Masanori Nishi, Sunil C. Kaul and Renu Wadhwa -- Mortalin Staining Pattern as a Reporter for Cell Based Anti-Cancer Drug Screening / Ran Gao, Zeenia Kaul, Tomoko Yaguchi and Renu Wadhwa -- Cell Internalizing Anti-mortalin Antibody as a Nanocarrier / Zeenia Kaul, Tomoko Yaguchi, Renu Wadhwa and Sunil C. Kaul.
  • 2011
    Finan, Dina; Pfeffer, Suzanne R.; Spudich, James A.; Straight, Aaron.
    Molecular motors are proteins that use energy from ATP hydrolysis to walk along cytoskeletal tracks, often transporting cargo as they move. A substantial amount of work has been done to study their catalytic domains, obtaining in vitro biochemical, structural, and biophysical measurements of motors' stepping behavior. Compared to this wealth of data, much less is known about how they actually work in cells. Regions of the motor proteins called cargo-binding domains are responsible for mediating their interactions with other molecules, but we know relatively little about what specific cargoes they associate with, the biochemical basis of these interactions, how they are regulated, and thus the precise functions of the motors in vivo. We have focused on two unconventional myosin motors in Drosophila, V and VI, both of which play important roles in fly development, and whose cargo-binding domains have been relatively unexplored. We use a proteomics approach to identify multiple novel binding partners for both myosins. The identities and biochemical characterization of a small fraction of the candidate binding proteins have begun to shed light on pathways in which the motors are likely to be involved. We have focused on roles for myosin VI in protein secretion and intracellular trafficking, and it seems likely that myosin V will play distinct roles in trafficking of ribonucleoprotein complexes. The discovery of binding partners is an important step towards understanding what motors do in cells, and we expect that this method will be useful for other motors in the future. We also hope that these findings will continue to elucidate biological roles of myosin V and VI.
  • 2011 Future Med
    Platanias, Leonidas C.
    mTOR inhibitors in cancer treatment / Leonidas C. Platanias -- Overview and mechanism of action of mTOR inhibitors / Stéphane Oudard -- mTOR inhibitors in renal cell carcinoma / Piotr J. Wysocki -- mTOR inhibitors in hepatocellular carcinoma / Gerhard Treiber & Thomas Wex -- mTOR inhibitors in ovarian cancer / Susana M. Campos -- mTOR inhibitors in breast cancer / Ingrid A. Mayer -- mTOR inhibitors in Hodgkin's and non-Hodgkin's lymphomas / Sonali M. Smith.
  • 2012 Springer Protocols
    McGuckin, Michael A.; Thornton, David J.
    Mucin methods : genes encoding mucins and their genetic variation with a focus on gel-forming mucins / Karine Rousseau and Dallas M. Swallow -- Gel-forming and cell-associated mucins : preparation for structural and functional studies / Julia R. Davies, Claes Wickstrom, and David J. Thornton -- Detecting, visualising, and quantifying mucins / Ceri A. Harrop, David J. Thornton, and Michael A. McGuckin -- Mass spectrometric analysis of mucin core proteins / Mehmet Kesimer and John K. Sheehan -- O-glycoprotein biosynthesis : site localization by edman degradation and site prediction based on random peptide substrates / Thomas A. Gerken -- Analysis of assembly of secreted mucins / Malin E.V. Johansson and Gunnar C. Hansson -- MUC1 membrane trafficking : protocols for assessing biosynthetic delivery, endocytosis, recycling, and release through exosomes / Franz-Georg Hanisch [and others] -- Glycomic work-flow for analysis of mucin o-linked Oligosaccharides / Catherine A. Hayes [and others] -- O-glycomics : profiling and structural analysis of mucin-type o-linked glycans / Isabelle Breloy -- O-glycoproteomics : site-specific o-glycoprotein analysis by CID/ETD electrospray ionization tandem mass spectrometry and top-down glycoprotein sequencing by in-source decay MALDI mass spectrometry / Franz-Georg Hanisch -- Analysing mucin degradation / Stephen D. Carrington [and others] -- Assessment of mucus thickness and production in situ / Lena Holm and Mia Phillipson -- Preservation of mucus in histological sections, immunostaining of mucins in fixed tissue, and localization of bacteria with FISH / Malin E.V. Johansson and Gunnar C. Hansson -- Ex vivo measurements of mucus secretion by colon explants / Jenny K. Gustafsson, Henrik Sjovall, and Gunnar C. Hansson -- Establishment of respiratory air-liquid interface cultures and their use in studying mucin production, secretion and function / David B. Hill and Brian Button -- Studying mucin secretion from human bronchial epithelial cell primary cultures / Lubna H. Abdullah [and others] -- Assessment of intracellular mucin content in vivo / Lucia Piccotti, Burton F. Dickey, and Christopher M. Evans -- Techniques for assessment of interactions of mucins with microbes and parasites in vitro and in vivo / Yong H. Sheng [and others] -- Assessing mucin expression and function in human ocular surface epithelia in vivo and in vitro / Pablo Argueso and Ilene K. Gipson.
  • 2013 Future Med
    Burchell, Joy M.; Taylor-Papadimitriou, Joyce.
    Mucins and cancer / Joyce Taylor-Papadimitriou & Joy M. Burchell -- Introduction to mucins in cancer / Michael A. McGuckin -- Changes in mucin-type O-linked glycosylation in cancer / Joy M. Burchell & Joyce Taylor-Papadimitriou -- Mucins as biomarkers in cancer / Kowa Chen, Ola Blixt & Hans H. Wandall -- Signaling from membrane mucins / Tze Wei Poh & Sandra J. Gendler -- MUC4 and membrane receptors in cancer / Kermit L. Carraway & Coralie A. Carothers Carraway -- MUC16, alias CA125 and ovarian cancer / Evangelia-Ourania Fourkala, Aleksandra Gentry-Maharaj & Usha Menon -- Mucins in metastasis / Sylvain Julien -- How mucins shape antigen-presenting cells to modify immune responses / Sandra J. van Vliet & Yvette van Kooyk -- MUC1 as a therapeutic target in cancer / Jean-Marc Limacher & Bruce Acres -- Index.
  • 2014 Springer Protocols
    Russell, David James.
    Dynamic programming / O. Ufuk Nalbantoglu -- Heuristic alignment methods / Osamu Gotoh -- Objective functions / Haluk Dogan and Hasan H. Otu -- Who watches the watchmen? An appraisal of benchmarks for multiple sequence alignment / Stefano Iantorno ... [et al.] -- BLAST and FASTA similarity searching for multiple sequence alignment / William R. Pearson -- Clustal omega, accurate alignment of very large numbers of sequences / Fabian Sievers and Desmond G. Higgins -- T-coffee : tree-based consistency objective function for alignment evaluation / Cedrik Magi ... [et al.] -- MAFFT : iterative refinement and additional methods / Kazutaka Katoh and Daron M. Standley -- Multiple sequence alignment using probcons and probalign / Usman Roshan -- Phylogeny-aware alignment with PRANK / Ari Loytynoja -- GramAlign : fast alignment driven by grammar-based phylogeny / David J. Russell -- Multiple sequence alignment with DIALIGN / Burkhard Morgenstern -- PicXAA : a probabilistic scheme for finding the maximum expected accuracy alignment of multiple biological sequences / Sayed Mohammad Ebrahim Sahraeian and Byung-Jun Yoon -- Multiple protein sequence alignment with MSAProbs / Yongchao Liu and Bertil Schmidt -- Large-scale multiple sequence alignment and tree estimation using SATe / Kevin Liu and Tandy Warnow -- PRALINE : a versatile multiple sequence alignment toolkit / Punto Bawono and Jaap Heringa -- PROMALS3D : multiple protein sequence alignment enhanced with evolutionary and three-dimensional structural information / Jimin Pei and Nick V. Grishin -- MSACompro : improving multiple protein sequence alignment by predicted structural features / Xin Deng and Jianlin Cheng.
  • 2014 Springer
    Deb, Sumitra; Deb, Swati Palit.
    This book provides the readers with an overview of research on p53, which has been shown to play a role in numerous crucial biological pathways in normal and cancer cells. Leading scientist in the field, who have all made direct contributions to the understanding of the molecular events underpinning p53 function, have been invited to contribute the various chapters, which discuss the current knowledge of the signaling cascades that are activated by mutations in p53 and overexpression of MDM2, frequently found in human cancer, and are major causes of oncogenesis. This book features chapters on the molecular basis of oncogenesis induced by gain of function mutation of p53, signaling pathways induced by MDM2 overexpression, control of mutant or wild-type p53 function by MDM2 and MDMX, p53 mutation in hereditary cancer, and structural aspects that activate mutant p53 which can be targeted by drug therapy. This book should be useful for scientists at all levels.
  • 2008 Springer
    Coluccio, Lynne M.
    The structural and functional diversity of the myosin family of actin-based molecular motors / M.S. Mooseker and B.J. Foth -- Myosin structure / K.C. Holmes -- The myosin family: Biochemical and kinetic properties / M. El-Mezgueldi and C.R. Bagshaw -- Myosin I / L.M. Coluccio -- Myosin II : Sarcomeric myosins, the motors of contraction in cardiac and skeletal muscles / C. Reggiani and R. Bottinelli -- Smooth-muscle myosin II / C.R. Cremo and D.J. Hartshorne -- Non-muscle myosin II / M. Conti, S. Kawamoto and R.S. Adelstein -- Class III myosins / A. Dosé, J. Lin-Jones and B. Burnside -- Myosin V / J.R. Sellers and L.S. Weisman -- Myosin VI : A multifunctional motor protein / F. Buss and J. Kendrick-Jones -- Myosin VII / A. El-Amraoui, A. Bahloul and C. Petit -- Plant myosins VIII, XI, and XIII / K. Yamamoto -- Class IX myosins / M. Bähler -- Myosin X / M.M. Divito and R.E. Cheney -- Myosin class XIV and other myosins in protists / K. Frénal, B.J. Foth and D. Soldati -- Myosin XVa / E.T. Boger ... [et al.].
  • 2011 Springer Protocols
    Toms, Steven A.; Weil, Robert J.
    MALDI-TOF serum profiling using semiautomated serum peptide capture with magnetic reversed phase (C18) beads / Jaco C. Knol and Connie R. Jimenez -- Proteomics of epithelial lining fluid obtained by bronchoscopic microprobe sampling / Lorenza Franciosi [and others] -- Protein identification using nano-HPLC-MS : ESI-MS and MALDI-MS interfaces / Rui Vitorino [and others] -- Three-dimensional peptide fractionation for highly sensitive nanoscale LC-based shotgun proteomic analysis of complex protein mixtures / Sricharan Bandhakavi [and others] -- Nanospray ion mobility mass spectrometry of selected high mass species / Iain Campuzano and Kevin Giles -- Nanoelectrospray-MSn of native and permethylated glycans / Christina Bleckmann, Hildegard Geyer, and Rudolf Geyer -- N-linked global glycan profiling by nanoLC mass spectrometry / Michael S. Bereman and David C. Muddiman -- Applications of nanoscale liquid chromatography coupled to tandem mass spectrometry in quantitative studies of protein expression protein-protein interaction, and protein phosphorylation / Metodi V. Metodiev -- Nano LC-MS/MS : a robust setup for proteomic analysis / Marco Gaspari and Giovanni Cuda -- Nanofluidic devices for rapid continuous-flow bioseparation / Pan Mao and Jianping Fu -- Quantifying attomole amounts of proteins from complex samples by nano-LC and selected reaction monitoring / Thomas Frohlich and Georg J. Arnold -- Sample preparation method for gold nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry / Yen-Hsiu Lin and Wei-Lung Tseng -- Nanostructured TiO2 thin films for phosphoproteomics studies with MALDI mass spectrometry / Federico Torta [and others] -- Nanofilament silicon for matrix-free laser desorption/ionization mass spectrometry / Chia-Wen Tsao and Don L. DeVoe -- Protein nanoarrays for high-resolution patterning of bacteria on gold surfaces / Cait Costello [and others] -- Engineered multifunctional nanotools for biological applications / Mohammed Ibrahim Shukoor [and others] -- Selective capture of phosphopeptides by zirconium phosphonate-magnetic nanoparticles / Liang Zhao, Renan Wu, and Hanfa Zou -- Nanowire biosensors for label-free, real-time, ultrasensitive protein detection / Gengfeng Zheng and Charles M. Lieber -- Generation of anti-infectome/anti-proteome nanobodies / Gholamreza Hassanzadeh-Ghassabeh, Dirk Saerens, and Serge Muyldermans -- Isolation, propagation, and analysis of biological nanoparticles / Michael P. Linnes [and others] -- Functionalized soluble nanopolymers for phosphoproteome analysis / Anton Iliuk [and others] -- Elucidating structural dynamics of integral membrane proteins on native cell surface by hydroxyl radical footprinting and nano LC-MS/MS / Yi Zhu, Tiannan Guo, and Siu Kwan Sze -- Application of electrostatic repulsion hydrophilic interaction chromatography to the characterization of proteome, glycoproteome, and phosphoproteome using nano LC-MS/MS / Piliang Hao, Huoming Zhang, and Siu Kwan Sze.
  • 2006 Springer
    Clerico, Aldo; Emdin, Michele.
  • 2015 Springer
    Vassallo, Neville.
    This edited volume concerns a group of devastating neurological disorders that share a common pathological mechanism, namely the aggregation and deposition of insoluble, proteinaceous lesions, termed 'amyloid'. Examples of cerebral amyloid disorders include common neurodegenerative diseases like Alzheimer's disease-related dementia and Parkinson's disease, as well as other less prevalent conditions like Huntington's disease, cerebral amyloid angiopathy and the transmissible prion disorders. A disease-modifying therapeutic agent is still lacking for all these diseases, and there are no approved therapies that target amyloid formation directly. Nevertheless, a large and complex group of natural aromatic compounds known as polyphenols are rapidly emerging as potentially potent anti-amyloidogenic agents. This book collectively presents a considerable body of experimental and epidemiological evidence from peer-reviewed scientific publications that support a role for natural compounds and herbal extracts in the chemoprevention and therapy of amyloidogenic disorders. Each contribution is written by scientific experts in the relevant field; chapters are devoted to Mediterranean diet and olive oil phenols, traditional Chinese medicine, herbal extracts, polyphenols (with a particular emphasis on epigallocatechin-3-gallate) and bi-flavonoids, amongst others. The topic of this book is relevant to a wide audience, from academia and university students in the biological and chemical sciences, to physicians and allied health professionals, as well as people working in the nutraceutical industry.
    Also available: Print – 2015
  • pt. A-C, 2012. ScienceDirect
    pt. B ScienceDirect
    pt. C ScienceDirect
    Hopwood, D. A.
  • 2012 Springer Protocols
    Nahar, Lutfun; Sarker, Satyajit D.
    An introduction to natural products isolation -- Initial and bulk extraction of natural products isolation -- Supercritical fluid extraction in natural products analyses -- Accelerated solvent extraction for natural products isolation -- Microwave-assisted extraction in natural products isolation -- An introduction to planar chromatography and its application to natural products isolation -- Isolation of natural products by low-pressure column chromatography -- Isolation of natural products by ion-exchange methods -- Separation of natural products by countercurrent chromatography -- Isolation of natural products by preparative high performance liquid chromatography (prep-HPLC) -- Isolation of natural products by preparative gas chromatography -- Hyphenated techniques and their applications in natural products analysis -- Extraction of plant secondary metabolites -- Isolation of marine natural products -- Isolation of microbial natural products -- Extraction and isolation of saponins -- Extraction and isolation of phenolic compounds -- Scaling-up of natural products isolation -- Follow-up of natural products isolation -- Natural products isolation in modern drug discovery programs.
  • 2012 Springer
    Lutz, Hans U.
    Naturally occurring IgM antibodies to oxidation-specific epitopes / Christoph J. Binder -- Naturally occurring autoantibodies to apoptotic cells / Keith B. Elkon and Gregg J. Silverman -- Naturally occurring antibodies directed against carbohydrate tumor antigens / Reinhard Schwartz-Albiez -- Naturally occurring antibodies as therapeutics for neurologic disease : can human monoclonal IgMs replace the limited resource IVIG? / Arthur E. Warrington ... [et al.] -- Nature and nurture of catalytic antibodies / Sudhir Paul ... [et al.] -- Naturally occurring autoantibodies in mediating clearance of senescent red blood cells / Hans U. Lutz -- Naturally occurring autoantibodies against ss-amyloid / Jan-Philipp Bach and Richard Dodel -- Multi-faceted role of naturally occurring autoantibodies in fighting pathogens / Hicham Bouhlal and Srini Kaveri -- Immunoregulation by naturally occurring and disease-associated autoantibodies : binding to cytokines and their role in regulation of T-cell responses / Claus H. Nielsen and Klaus Bendtzen -- Modulation of dendritic cells and regulatory T cells by naturally occurring antibodies / Jaap Kwekkeboom -- Control of B cells expressing naturally occurring autoantibodies / Jean Louis Pasquali and Thierry Martin -- Granulocyte death regulation by naturally occurring autoantibodies / Stephan Von Gunten and Hans-Uwe Simon -- Naturally occurring autoantibodies mediate ischemia/reperfusion-induced tissue injury / Sherry D. Fleming -- How immune complexes from certain IgG NAbs and any F(ab')2 can mediate excessive complement activation / Hans U. Lutz -- The natural autoantibody repertoire in newborns and adults : a current overview / Asaf Madi ... [et al.] -- Antibody polyspecificity : what does it matter? / Jordan D. Dimitrov, Anastas D. Pashov, Tchavdar L. Vassilev -- Positive and negative selection of natural autoreactive B cells / Richard R. Hardy and Kyoko Hayakawa -- Naturally occurring antibodies/autoantibodies in polyclonal immunoglobulin concentrates / Peter J. Späth and Hans U. Lutz.
    Also available: Print – 2012
  • 2015 Springer
    Blatch, Gregory L.
  • 2008 Springer
    Ritsner, Michael S.; Weizman, Abraham.
  • 2011 Springer
    Gallo, Gianluca; Lanier, Lorene M.
    1. Introduction to the neurobiology of actin -- 2. The neuronal actin cytoskeleton and the protrusion of lamellipodia and filopodia -- 3. Regulation of actin filaments during neurite extension and guidance -- 4. Functions of myosin motor proteins in the nervous system -- 5. Microtubule-actin interactions during neuronal development -- 6. Working with actin: methodological approaches for the study of actin in neurons -- 7. Functions of the actin cytoskeleton in the early embryology of the nervous system -- 8. Actin cytoskeletal regulation in neuronal migration -- 9. Actin and neuronal polarity -- 10. Actin at the synapse: contribution to pre- and postsynaptic functions -- 11. Actin and diseases of the nervous system -- 12. Signal transduction pathways: from receptor to the actin cytoskeleton -- Index.
  • 2015 Springer
    Fonta, Caroline; Négyessy, László.
    Phosphatases such as TNAP are fundamental in regulating cellular, and consequently numerous body functions. TNAP is a ubiquitous enzyme with a wide spectrum of substrates and specificity regulation at the cellular level and the lack of TNAP activity is a lethal condition. Recent findings of a highly specific regional, laminar and subcellular localization of TNAP in the cerebral cortex indicates that in addition to its metabolic and skeletal functions, TNAP also plays a role in regulating cerebral functions, most probably cognition. In fact, TNAP disturbance could result in complex diseases such as epilepsy, developmental retardation and Alzheimer disease. Available data suggest that, regarding brain functions, TNAP is a potentially important target of clinical research. The proposed book aims to provide an overview of our current understanding of the functions of TNAP in the brain.
  • 2010 Springer
    Geary, Timothy G.; Maule, Aaron G.
    Receptor-based discovery strategies for insecticides and parasiticides: a review / Debra Woods ... [et al.] -- Nonpeptide ligands for peptidergic G protein-coupled receptors / Timothy G. Geary -- Interaction of mimetic analogs of insect kinin neuropeptides with arthropod receptors / Ronald J. Nachman and Patricia V. Pietrantonio -- Neuropeptides in helminths: occurrence and distribution / Nikki J. Marks and Aaron G. Maule -- Neuropeptide physiology in helminths / Angela Mousley ... [et al.] -- Neuropeptide gene families in Caenorhabditis elegans / Chris Li and Kyuhyung Kim -- Control of nematode parasites with agents acting on neuromusculature systems: lessons for neuropeptide ligand discovery / Richard J. Martin and Alan P. Robertson -- Neuropeptide signaling in insects / Miriam Altstein and Dick R. Nassel -- Neuropeptide physiology in insects / William G. Bendena -- Neuropeptide biology in Drosophila / Elke Clynen ... [et al.] -- Neuropeptide receptors as possible targets for development of insect pest control agents / Matthias B. Van Hiel ... [et al.].
    Also available: Print – 2010
  • 2012 CRCnetBASE
    Nyberg, F.
    "Preface: Over the past four decades, the number of peptides identified as neurotransmitters or neuromodulators in the central and peripheral nervous systems has significantly increased. These compounds, known as neuropeptides, have been recognized for playing an important role in the communication between cells in a variety of neuronal networks. Although, a considerable number of neuropeptides have been characterized so far, their quantity is limited compared to the number of precursor proteins that are actually found to be expressed in cells of the nervous system. Numerous studies have confirmed that neuropeptides are involved in a number of biological activities. These include modulation of brain reward, pain processing, and immune response, as well as neuroendocrine regulations, control of neurovegetative functions, and trophic effects. In recent years, it has become evident that the role of neuropeptides as fast-acting neurotransmitters is challenged by the observation that many of them may act as growth factors by stimulating cell proliferation and slow-acting mitogenesis. It has thus been demonstrated that a number of neuroactive peptides, such as pituitary adenylate cyclase-activating polypeptide (PACAP), adrenocorticotrophin (ACTH), opioid peptides, somatostatin, and substance P, may modulate proliferation and cell viability. Some peptides act as stimulatory factors on brain circuits involved in cognition, whereas others may act as inhibitory factors. Accordingly, many neuropeptide systems appear as important targets for neuroprotective drugs and drugs that promote neuroregeneration"--Provided by publisher.
  • 2011 Springer Protocols
    Li, Ka Wan.
    Neuroproteomics : deciphering brain function and disorders / Ka Wan Li -- Dissection of rodent brain regions / Sabine Spijker -- Subcellular fractionation of brain tissue using free-flow electrophoresis / Markus Islinger ... [et al.] -- Isolation of synapse subdomains by subcellular fractionation using sucrose density gradient centrifugation / Tatsuo Suzuki -- Enrichment of plasma membranes from small brain samples by aqueous polymer two-phase systems / Jens Schindler -- Identification and characterization of protein complexes from total brain and synaptoneurosomes : heterogeneity of molecular complexes in distinct subcellular domains / Silvia De Rubeis and Claudia Bagni -- Analysis of protein complexes by 2d blue native/SDS-PAGE and antibody-shift assay / Dong Yang ... [et al.] -- Two-dimensional gel electrophoresis-based proteomic analysis of brain synapses / Karl-Heinz Smalla and Ursula Wyneken -- Two-dimensional BAC-SDS polyacrylamide gel electrophoresis for the fractionation and identification of synaptic vesicle proteins and the presynaptic active zone / Joern Barth and Walter Volknandt -- iTRAQ-based LC-LC MALDI TOF/TOF MS quantitative analysis of membrane proteins from human glioma / Uros Rajcevic -- OFFGEL-isoelectric focusing fractionation for the analysis of complex proteome / Emilie Ernoult and Catherine Guette -- 1D-PAGE/LC-ESI linear ion trap orbitrap MS approach for the analysis of synapse proteomes and synaptic protein complexes / Ning Chen, Roel C. vd Schors, and August B. Smit -- SDS-PAGE immunoblot analysis / Patricia Klemmer -- Phosphoproteomics by highly selective IMAC protocol / Chia-Feng Tsai ... [et al.] -- Global analysis of ubiquitination / David Meierhofer and Peter Kaiser -- High-throughput high-content functional image analysis of neuronal proteins implicated in Parkinson's disease / Eva Blaas and Ronald E. van Kesteren -- Neuropeptidomics of the mammalian brain / Fang Xie, Elena V. Romanova, and Jonathan V. Sweedler -- In-depth analysis of the cerebrospinal fluid proteome and biomarker discovery : abundant protein depletion sample pretreatment method / Silvina A. Fratantoni and Connie R. Jimenez -- Lipidomics of the nervous system : phospholipidomics by liquid chromatography coupled to mass spectrometry or tandem mass spectrometry / Su Chen -- Bioinformatics procedures for analysis of quantitative proteomics experiments using iTRAQ / Pim van Nierop and Maarten Loos -- Statistical analysis of spectral count data generated by label-free tandem mass spectrometry-based proteomics / Thang V. Pham and Connie R. Jimenez.
  • 2016 Springer
    Fuhrmann, U.; Jaroch, S.; Nielsch, Ulrich.
    This volume gives an overview of state of the art technologies and future developments in the field of preclinical pharmaceutical research. A balanced mix of experts from academia and industry give insight in selected new developments in the drug discovery pathway. The topics cover the different parts of the drug discovery process, starting with new developments in the target identification and validation area. The lead generation part as a next step focuses on the requirements and technologies to identify new small molecules as lead compounds for further optimization; in a second section the technologies to identify biologics as leads are addressed. The final part focuses on the pharmacological models and technologies to characterize new compounds and the impact of biomarkers to facilitate the transfer of drug candidates into the development phase.
  • 2012 Springer
    Ma'ayan, Avi; MacArthur, Ben D.
    Gene Expression Profiling and Regulatory Networks in Single Cells / Fumio Arai, Kentaro Hosokawa, Yoshiko Matsumoto, Hirofumi Toyama and Toshio Suda -- Cell Fate Regulatory Networks / Sonya J. Ridden and Ben D. MacArthur -- Noise in Regulatory Networks / Srinandan Dasmahapatra -- Generalised Modelling in Systems Biology / Thilo Gross -- Relative Quantification Mass Spectrometry Using iTRAQ Isobaric Tags / Richard D. Unwin, Emma Carrick and Anthony D. Whetton -- Deciphering Protein Complexes and Protein Interaction Networks for Stem Cell Pluripotency / Jianlong Wang -- Omics Era in Stem Cell Research: Data Integration of Multi-regulatory Layers / Ana Sevilla, Benjamin Trinite and Ihor R. Lemischka -- High Throughput Affinity Purification and Mass Spectrometry to Determine Protein Complex Interactions / Anna Malovannaya, Rainer B. Lanz, Bert W. O'Malley and Jun Qin -- Network Pharmacology to Aid the Drug Discovery Process / Ruth Dannenfelser, Huilei Xu, Catherine Raimond and Avi Ma'ayan -- What Can Networks Do for You? / Bernhard M. Schuldt, Franz-Josef Müller and Andreas A. Schuppert.
  • 2007 Springer
    Durlach, Jean; Morii, H.; Nishizawa, Yoshiki.
    Overview -- Magnesium homeostasis -- Nutrition -- Epidemiology -- Exercise -- Metabolic syndrome -- Cardiovascular disease -- Skeletal diseases and calcium metabolism -- Kidney -- Neurology -- Magnesium in dental medicine -- Magnesium in neurology and psychiatry -- Veterinary medicine.
  • 2006 Springer
    Liou, Hsiou-Chi.
  • 2010 ScienceDirect
    Ignarro, Louis J.
    Section I: -- Chemical biology: Determinants of nitric oxide chemistry: inpact of cell signaling processes / Douglas D. Thomas, Wilmarie Flores-Santana, Christopher H. Switzer -- Nitric oxide redox biochemistry in lipid environments / Andrés Trostchansky, Matías N. Möller, Silvina Bartesaghi, Horacio Botti, Ana Denicola, Rafael Radi and Homero Rubbo -- Mechanisms and biological consequences of peroxynitrite-dependent protein oxidation and nitration / Madia Trujillo, Beatriz Alvarez, José M. Souza, Natalia Romero, Laura Castro, Leonor Thomson and Rafael Radi -- Systems approaches to unraveling nitric oxide response netwoeks in prokaryotes / Laura R. Jarboe, Daniel R. Hyduke and James C. Liao. Section II: -- Principles of biology: (A. Nitric oxide synthases) Uncoupling of endothelial nitric oxide synthase in cardiovascular disease and its pharmacological reversal / Ulrich Förstermann -- Tetrahydrobiopterin: an essential cofactor for nitric oxide syntahases and amino acid hydroxylases / Christina E. Higgins and Steven S. Gross -- Regulation of the expression of inducible nitric oxide synthase / Hartmut Kleinert, Julia Art and Andrea Pautz -- Molecular regulation of inducible nitric oxide synthase / Qiang Du and David A. Geller -- (B. Guanylate cyclase and cyclic GMP) Soluble guanylate cyclase: allosteric activtion and redox regulation / Adrian J. Hobbs and Johannes-Peter Stasch (C. Nitric oxide signaling) Untargeted discovery of nitric oxide-modified proteins / Ruba Deeb, Tal Nuriel and Steven S. Gross -- Fatty acid transduction of nitric oxide signaling: cyclooxygenases, lipoxygenases and nitro-fatty acids / Marsha P. Cole and Bruce A. Freeman -- Nitric oxide signaling in vascular cells is regulated through CD47 by thrombospondin-1 / Jeffery S. Isenberg, William A. Frazier and David D. Roberts -- The regulation of cell energetics and mitochondrial signaling by nitric oxide / Alberto Boveris, Maria Cecilia Carreras and Juan Jose Poderoso -- (D. Regulation of nitric oxide production) Nitric oxide-asymmetric dimedthylarginine system in endothelial cell senescence / Fortunato Scalera and Stefanie M. Bode-Böger -- (E. Nitric oxide and remodeling) The role of nitric oxide in apoptosis and autophagy: biochemical and computational studies / P. A. Loughran, E. Z. Bagci, R. Zamora, Y. Vodovotz and T. R. Billiar -- (F. Nitrite and nitrate) Nitric oxide formation from inorganic nitrate and nitrite / Jon O. Lundberg and Eddie Weitzberg -- Mechanisms of nitrite reduction in ischemia in the cardiovascular system / Andrew Webb and Amrita Ahluwalia -- Nitrite therapy for ischemic syndromes / Christopher G. Kevil and David J. Lefer -- Nitrite and heme globins: reaction mechanisms and physiological targets / Sruti Shiva, Sheila Frizzill and Mark T. Gladwin. Section III: -- Principles of pathobiology (A. Nitric oxide and cardiovascular function) Nitric oxide in vascular damage and regeneration / Claudio Napoli, Lilach O. Lerman, Maria Luisa Balestrieri and Louis J. Ignarro -- Free radicals as atherosclerotic risk in relation to nitric oxide / Toshio Hayashi and Akihisa -- The role of oxidative stress in endothelial dysfunction and vascular inflammation / Elena Osto and Francesco Cosentino -- (B. Pathophysiological mechanism of nitric oxide) Nitric oxide, oxidative stress, immune response and critical care / Claudio Napoli, Ettore Crimi, Sharon Williams-Ignarro, Filomena de Nigris and Louis J. Ignarro -- Reactive metabolites of oxygen and nitrogen in liver ischemia and reperfusion injury / Ian N. Hines and Matthew B. Grisham -- Nitric oxide in airway inflammation / Yong Chul Lee, So Ri Kim, Eun-Kyeong Jo, Hyun-Ock Pae and Hun-Taeg Chung -- Novel therapeutic applications of nitric oxide in the inhibition of tumor malignancy and reversal of resistance / Benajmin Bonavida.
  • 2011 Springer Protocols
    Coulter, Jonathan A.; McCarthy, Helen O.
    Nitric oxide physiology and pathology / David G. Hirst and Tracy Robson -- Ionizing radiation-induced DNA strand breaks and [gamma]-H2AX foci in cells exposed to nitric oxide / Kai Rothkamm and Susanne Burdak-Rothkamm -- Determination of S-nitrosothiols in biological fluids by chemiluminescence / Enika Nagababu and Joseph M. Rifkind -- Measurement of nitrite in blood samples using the ferricyanide-based hemoglobin oxidation assay / Barbora Piknova and Alan N. Schechter -- Selective fluorescent activation for bioimaging the expression of nitric oxide in cellular and in vivo systems / Junfeng Zhang and Hao Hong -- Real-time measurement of murine hippocampus NO levels in response to cerebral ischemia/reperfusion / Xiaoxiang Zheng, Kezhou Liu, and Yong Yang -- Detection of low levels of nitric oxide using an electrochemical sensor / Yong Chool Boo ... [et al.] -- Determination of the scavenging capacity against reactive nitrogen species by automatic flow injection-based methodologies / Marcela A. Segundo ... [et al.] -- Aqueous measurement of nitric oxide using membrane inlet mass spectrometry / David N. Silverman and Chingkuang Tu -- Quantum cascade laser technology for the ultrasensitive detection of low-level nitric oxide / Angela Elia ... [et al.] -- Determination of in vivo nitric oxide levels in animal tissues using a novel spin trapping technology / Anatoly F. Vanin and Alexander A. Timoshin -- [Beta] cell protection by inhibition of iNOS through lentiviral vector-based strategies / Sean O. Hynes, Cillian McCabe, and Timothy O'Brien -- Characterization of nitric oxide delivery systems produced by various nanotechnologies / Chi H. Lee -- Nitric oxide releasing nanoparticle synthesis and characterization / George Han, Adam J. Friedman, and Joel M. Friedman -- NOS antagonism using viral vectors as an experimental strategy : implications for in vivo studies of cardiovascular control and peripheral neuropathies / Beihui Liu ... [et al.]
  • pt. E-F, 2005-2008. ScienceDirect
    pt. F, 2008 ScienceDirect
    Cadenas, Enrique; Packer, Lester.
  • 2008 ScienceDirect
    Cadenas, Enrique; Packer, Lester.
    Also available: Print – 2008
  • 2011 Springer Protocols
    Ribbe, Markus W.
    Historic overview of nitrogenase research / Yilin Hu and Markus W. Ribbe -- Mechanism of mo-dependent nitrogenase / Zhi-Yong Yang, Karamatullah Danyal, and Lance C. Seefeldt -- Assembly of nitrogenase MoFe protein / Chi-Chung Lee -- Genomic analysis of nitrogen fixation / Ina P. O'Carroll and Patricia C. Dos Santos -- Enzymatic systems with homology to nitrogenase / Jurgen Moser and Markus J. Brocker -- Molecular biology and genetic engineering in nitrogen fixation / Patricia C. Dos Santos -- Purification of nitrogenase proteins / Jared A. Wiig [and others] -- Assays of nitrogenase reaction products / William E. Newton and Michael J. Dilworth -- Methods for nitrogenase-like dark operative protochlorophyllide oxidoreductase / Jurgen Moser and Markus J. Brocker -- X-ray crystallography / Lauren E. Roth and F. Akif Tezcan -- X-ray absorption spectroscopy / Serena DeBeer -- Small angle x-ray scattering spectroscopy / David W. Mulder and John W. Peters -- Electron paramagnetic resonance spectroscopy / Karamatullah Danyal, Zhi-Yong Yang, and Lance C. Seefeldt -- Magnetic circular dichroism spectroscopy / Brian J. Hales -- Mössbauer spectroscopy / Boi Hanh Huynh -- Protocols for cofactor isolation of nitrogenase / Aaron W. Fay [and others] -- Techniques for functional and structural modeling of nitrogenase / Patrick L. Holland -- Nitrogenase structure and function relationships by density functional theory / Travis V. Harris and Robert K. Szilagyi -- Modeling the MoFe nitrogenase system with broken symmetry density functional theory / Gregory M. Sandala and Louis Noodleman.
  • 2014 Springer
    Fabbri, Muller.
    Introduction / Muller Gabbri -- Biogenesis and physiology of microRNAs / Carlos A. Melo and Sonia A. Melo -- Detecting noncoding RNA expression : from arrays to next-generation sequencing / Catalina Perdomo, Joshua Campbell, and Frank Schembri -- MicroRNAs in solid tumors / Jin Wang and Subrata Sen -- MicroRNAs in hematologic malignancies / Johannes Bloehdorn ... [et al.] -- miRNAs as cancer biomarkers / Edward R. Sauter -- Unraveling the complex network of interactions between noncoding RNAs and epigenetics in cancer / Veronica Davalos and Manel Esteller -- MicroRNA SNPs in cancer / Yujing LI and Peng Jin -- Bioinformatics approaches to the study of MircoRNAs / Ravi Gupta and Ramana V. Davuluri -- Beyond miRNAs : role of other noncoding RNAs in cancer / Roxana S. Redis and George A. Calin -- Translational implication for noncoding RNA in cancer / Duaa Dakhallah, Melissa Piper, and S. Patrick Nana-Sinkam.
  • 2012 Springer
    Bitan, Gal; Rahimi, Farid.
  • 2009 ScienceDirect
    Abelson, John N.; Muir, Tom W.
    Also available: Print – 2009
  • 2012 Springer
    Brouns, Inge.
  • 2015 Springer Protocols
    Allen, Bruce G.; Hébert, Terence E.
    Studies of intracellular angiotensin II / Richard N. Re and Julia L. Cook -- Single-cell microinjection coupled to confocal microscopy to characterize nuclear membrane receptors in freshly isolated cardiomyocytes / Clémence Merlen and Jonathan Ledoux -- Design and application of light-activated probes for cellular signaling / David Chatenet, Steve Bourgault, and Alain Fournier -- Using caged ligands to study intracrine endothelin signaling in intact cardiac myocytes / Clémence Merlen, Louis R. Villeneuve, and Bruce G. Allen -- Quantification of catecholamine uptake in adult cardiac myocytes / Erika F. Dahl, Casey D. Wright, and Timothy D. O'Connell -- Characterization of the interaction between the prostaglandin D2 DP1 receptor and the intracellular L-Prostaglandin D synthase / Chantal Binda and Jean-Luc Parent -- Isolation and study of cardiac nuclei from canine myocardium and adult ventricular myocytes / Artavazd Tadevosyan, Bruce G. Allen, and Stanley Nattel -- High resolution imaging and function of nuclear G protein-coupled receptors (GPCRs) / Vikrant K. Bhosle [and others] -- Biochemical fractionation of membrane receptors in the nucleus / Ying-Nai Wang ... [et at.] -- Functional G protein-coupled Receptors on nuclei from brain and primary cultured neurons / Yuh-Jiin I. Jong and Karen L. O'Malley -- Automated microscopy of cardiac myocyte hypertrophy : a case study on the role of intracellular [alpha]-adrenergic receptors / Karen A. Ryall and Jeffrey J. Saucerman -- Measuring intranuclear and nuclear envelope [Ca2+] vs. cytosolic [Ca2+] / Senka Ljubojević and Donald M. Bers -- Assessing GPCR and G protein signaling to the nucleus in live cells using fluorescent biosensors / Julie Bossuyt and Donald M. Bers -- Tandem affinity purification to identify cytosolic and nuclear G [beta-gamma]-interacting proteins / Rhiannon Campden [and others] -- Examining the effects of nuclear GPCRs on gene expression using isolated nuclei / George Vaniotis [and others] -- Trafficking and function of GPCRs in the endosomal compartment / Davide Calebiro [and others].
  • 2005 Springer
    Citovsky, Vitaly; Tzfira, Tzvi.
    Nuclear import and export of mammalian viruses / Michael Bukrinsky -- Nuclear import of DNA / David A. Dean and Kerimi E. Gokay -- Nuclear transport research methods / Jose Omar Bustamante. Regulation of nuclear import and export of proteins in plants and its role in light signal transduction / Stefan Kircher ... [et al.] -- Nuclear export: shuttling across the nuclear pore / John A. Hanover and Dona C. Love -- Nuclear protein import: distinct intracellular receptors for different types of import substrates / David A. Jans and Jade K. Forwood -- The molecular mechanisms of mRNA export / Tetsuya Taura, Mikiko C. Siomi and Haruhiko Siomi -- Structure of the nuclear pore / Michael Elbaum -- Integral proteins of the nuclear pore membrane / Merav Cohen, Katherine L. Wilson and Yosef Gruenbaum -- Subnuclear trafficking and the nuclear matrix / Iris Meier -- Nuclear import and export signals / Toshihiro Sekimoto, Jun Katahira and Yoshihiro Yoneda -- Nuclear import of plant proteins / Glenn R. Hicks -- Nuclear import of agrobacterium T-DNA / Tzvi Tzfira, Benoit Lacroix and Vitaly Citovsky --
  • 2002 ScienceDirect
    Laudet, Vincent; Gronemeyer, H.
  • 2003 ScienceDirect
    Mangelsdorf, David J.; Russell, David W.
    Also available: Print – 2003
  • 2010 Springer
    Bunce, Chris M.; Campbell, Moray J.
    Nuclear receptors an introductory overview / C.M. Bunce and M.J. Campbell -- What does evolution teach us about nuclear receptors? / G. Markov, F. Bonneton and V. Laudet -- Functions of nuclear receptors in insect development / D. Martín -- The glucocorticoid receptor / R.H. Oakley and J.A. Cidlowski -- Estrogen receptors: Their actions and functional roles in health and disease / S. Nilsson and J.-Å. Gustafsson -- Androgen receptor / J.T. Dalton and W. Gao -- Thyroid hormone receptors / B. Vennström, H. Liu and D. Forrest -- The vitamin D receptor (NR1I1) / O. Maguire and M.J. Campbell -- Retinoic acid receptors / A. Cras, F. Guidez and C. Chomienne -- PPARs: Important regulators in metabolism and inflammation / L.M. Sanderson and S. Kersten -- Xenobiotic receptors CAR and PXR / C. Klaassen and H. Lu -- Fxr / Y. Wang ... [et al.] -- Physiological functions of TR2 and TR4 orphan nuclear receptor / S. Liu ... [et al.] -- Nuclear receptors and ATP dependent chromatin remodeling: A complex story / C.J. Burd and T.K. Archer -- Non-genomic action of sex steroid hormones / A. Migliaccio, G. Castoria and F. Auricchio -- Ligand regulation and nuclear receptor action / M. Hewison -- New insights to nuclear receptor gene regulation from analysis of their response elements in target genes / C. Carlberg -- Systems biology: Towards realistic and useful models of molecular networks / F.J. Bruggeman ... [et al.].
  • 2016 Springer Protocols
    Mayer, Günter.
    Selection of aptamers for metabolite sensing and construction of optical nanosensors / Yi Long ... [et al.] -- SELEX of cell-specific RNA aptamers / Katharina Berg, Eileen Magbanua, and Ulrich Hahn -- Developing aptamers by cell-based SELEX / Silvia Catuogno, Carla Lucia Esposito, and Vittorio de Franciscis -- DNA aptamer generation by genetic alphabet expansion SELEX (ExSELEX) using an unnatural base pair system / Michiko Kimoto, Ken-ichiro Matsunaga, and Ichiro Hirao -- Capillary electrophoresis for the selection of DNA aptamers recognizing activated protein C / Nasim Shahidi Hamedani and Jens Müller -- Preparation of SELEX samples for next-generation sequencing / Fabian Tolle and Günter Mayer -- Next-generation analysis of deep sequencing data : bringing light into the black box of SELEX experiments / Michael Blank -- Aptamer binding studies using MicroScale thermophoresis / Dennis Breitsprecher ... [et al.] -- Label-free determination of the dissociation constant of small molecule-aptamer interaction by isothermal titration calorimetry / Marc Vogel and Beatrix Suess -- Applications of aptamers in flow and imaging cytometry / Isis C. Nascimento ... [et al.] -- In vitro and in vivo imaging of fluorescent aptamers / Ioanna Théodorou ... [et al.] -- Crystallographic pursuit of a protein-RNA aptamer complex / John J. G. Tesmer -- Voltammetric aptasensor based on magnetic beads assay for detection of human activated protein C / Arzum Erdem, Gulsah Congur, and Ece Eksin -- Apta-PCR / Alessandro Pinto ... [et al.] -- Aptamer-based enzyme capture assay for measurement of plasma thrombin levels / Jens Müller ... [et al.] -- Application of aptamers in histopathology / Sarah Shigdar ... [et al.] -- Aptamer stainings for super-resolution microscopy / Maria Angela Gomes de Castro, Burkhard Rammner, and Felipe Opazo -- Synthesis and characterization of aptamer-targeted SNALPs for the delivery of siRNA / Samantha E. Wilner and Matthew Levy -- Screening of genetic switches based on the twister ribozyme motif / Michele Felletti, Benedikt Klauser, and Jörg S. Hartig.
  • 2014 Springer
    Ferapontova, Elena; Gothelf, Kurt V.; Kjems, Jørgen.
    This volume on nucleic acid nanotechnology offers authoritative, up-to-date and comprehensive coverage of nanotechnological studies and applications of nucleic acids. It provides reviews of various aspects of nucleic acid nanotechnology, each written by an internationally leading expert in the field, and presents state-of-the-art and recent advances in nucleic acid synthetic modifications, nanoscale design, manipulation and current and future applications in bioengineering, medicine, electronics, genetic analysis, chemistry, molecular biology, surface and material sciences. It examines how nucleic acid research is merging with nanotechnology, allowing the nanoscale properties of nucleic acid to be exploited in performing challenging nanotechnological tasks, from nanorobotics and nanosensing to nucleic acid computing. This book will above all benefit anyone who is interested in nanotechnological concepts of nucleic acid design and applications, and offers a valuable resource for teaching these concepts. It is essential reading for a broad audience of scientists both in academia and industry who wish to expand their expertise on the potential of nucleic acid functions and applications.
  • 2014 Springer
    Murakami, Katsuhiko S.; Trakselis, Michael A.
    This book provides a review of the multitude of nucleic acid polymerases, including DNA and RNA polymerases from Archea, Bacteria and Eukaryota, mitochondrial and viral polymerases, and other specialized polymerases such as telomerase, template-independent terminal nucleotidyl transferase and RNA self-replication ribozyme. Although many books cover several different types of polymerases, no book so far has attempted to catalog all nucleic acid polymerases. The goal of this book is to be the top reference work for postgraduate students, postdocs, and principle investigators who study polymerase.
  • 2011 Springer
    Olson, Mark O. J.
  • 2005 CRCnetBASE
    Monir-Vaghefi, Seyed-Morteza.
  • pt. A-B, 2012. ScienceDirect
    pt. B ScienceDirect
    Allis, C. David; Wu, Carl.
  • pt. A-E, 1992-2004. ScienceDirect
    pt. B, 1994 ScienceDirect
    pt. C, 2000 ScienceDirect
    pt. D, 2004 ScienceDirect
    pt. E, 2004 ScienceDirect
    Brand, Ludwig; Johnson, Michael L.
  • 2011 Springer
    Prinz, Heino.
  • 2009 CRCnetBASE
    Choi, Sang-Woon; Friso, Simonetta.
    Methylation on the nucleosome / Da Jia and Xiaodong Cheng -- Mammalian DNA methyltransferases / Michael J. Boland and Judith K. Christman -- Chromatin remodeling and histone modifications / Gavin R. Schnitzler -- Nutrients and DNA methylation / Sang-Woon Choi, Roberto Corrocher, and Simonetta Friso -- Nutrients, histone modifications, and chromatin remodeling in chronic inflammation / Barbara Delage and Roderick H. Dashwood -- Nutrients, epigenetics, and embryonic development / Craig A. Cooney -- Nutrients, epigenetics, and aging / John C. Mathers and Dianne Ford -- Nutrition, epigenetics, and cancer / Sharon A. Ross -- Conclusions and future perspectives / Sang-Woon Choi and Simonetta Friso.
  • 2012 CRCnetBASE
    Ray, Abhijit; Srivastava, Punit Kumar.
    "Incidences of inflammatory airway diseases are on the rise across the world. Existing therapeutic options are ineffective, unsafe, and expensive, and severe cases are nonresponsive to conventional therapy. Therefore, it is imperative that research be undertaken to discover new treatment options. Obstructive Airway Diseases: Role of Lipid Mediators discusses clinically successful and potential lipid targets that can make a difference in treating some of the most intractable disease states."--Publisher description.
  • 2008 Springer Protocols
    Pflieger, Delphine; Rossier, Jean.
    Analysis of organelles by on-line two-dimensional liquid chromatography-tandem mass spectrometry / Edwin P. Romijn and John R. Yates III -- Purification and proteomic analysis of chloroplasts and their sub-organellar compartments / Daniel Salvi ... [et al.] -- Proteomic analysis of the stacked golgi complex / Mark S. Lowenthal, Kathryn E. Howell, and Christine C. Wu -- Purification of Saccharomyces cerevisiae mitochondria by zone electrophoresis in a free flow device / Hans Zischka ... [et al.] -- Preparation of respiratory chain complexes from Saccharomyces cerevisiae wild-type and mutant mitochondria : activity measurement and subunit composition analysis / Claire Lemaire and Geneviève Dujardin -- Mitochondrial proteomics : analysis of a whole mitochondrial extract with two-dimensional electrophoresis / Thierry Rabilloud -- Purification and proteomic analysis of the mouse liver mitochondrial inner membrane / Sandrine Da Cruz and Jean-Claude Martinou -- Subcellular fractionation and proteomics of nuclear envelopes / Laurence Florens, Nadia Korfali, and Eric C. Schirmer -- Purification and proteomic analysis of a nuclear-insoluble protein fraction / Tsuneyoshi Horigome, Kazuhiro Furukawa, and Kohei Ishii -- Preparation methods of human metaphase chromosomes for their proteome analysis / Kiichi Fukui, Hideaki Takata, and Susumu Uchiyama -- Purification and proteomic analysis of plant plasma membranes / Erik Alexandersson ... [et al.] -- Protocol to enrich and analyze plasma membrane proteins from frozen tissues / Jacek R. Wiśniewski -- Proteomic characterization of membrane protein topology / Adele R. Blackler and Christine C. Wu -- Free flow isoelectric focusing : a method for the separation of both hydrophilic and hydrophobic proteins of rat liver peroxisomes / Markus Islinger and Gerhard Weber -- Use of gas-phase fractionation to increase protein identifications : application to the peroxisome / Jacob Kennedy and Eugene C. Yi -- Purification and proteomic analysis of lysosomal integral membrane proteins / Huiwen Zhang ... [et al.] -- Affinity purification of soluble lysosomal proteins for mass spectrometric identification / Sylvie Kieffer-Jaquinod ... [et al.] -- Purification and proteomic analysis of synaptic vesicles / Holly D. Cox and Charles M. Thompson -- Purification and proteomics analysis of pancreatic zymogen granule membranes / Xuequn Chen and Philip C. Andrews -- Isolation and proteomic analysis of chlamydomonas centrioles / Lani C. Keller and Wallace F. Marshall -- Purification and proteomic analysis of 20S proteasomes from human cells / Marie-Pierre Bousquet-Dubouch ... [et al.] -- Characterization of E. coli ribosomal particles : combined analysis of whole proteins by mass spectrometry and of proteolytic digests by liquid chromatography-tandem mass spectrometry / Isabelle Iost ... [et al.] -- Assessment of organelle purity using antibodies and specific assays : the example of the chloroplast envelope / Daniel Salvi ... [et al.] -- Identifying bona fide components of an organelle by isotope-coded labeling of subcellular fractions : an example in peroxisomes / Marcello Marelli, Alexey I. Nesvizhskii, and John D. Aitchison -- Determination of genuine residents of plant endomembrane organelles using isotope tagging and multivariate statistics / Kathryn S. Lilley and Tom P.J. Dunkley -- Quantitative proteomic analysis to profile dynamic changes in the spatial distribution of cellular proteins / Wei Yan, Daehee Hwang, and Ruedi Aebersold -- Use of transcriptomic data to support organelle proteomic analysis / Wallace F. Marshall.
  • 2007 Springer
    Martin, William F.; Müller, Miklós.
    The road to hydrogenosomes -- Mitochondria: key to complexity -- Origin, function,and transmission of mitochondria -- Mitochondria and their host: morphology to molecular phylogeny -- Anaerobic mitochondria: properties and origins -- Iron-sulfur proteins and iron-sulfur cluster assembly in organisms with hydrogenosomes and mitosomes -- Hydrogenosomes (and related organelles, either) are not the same -- The chimeric origin of mitochondria: photosynthetic cell enslavement, gene-transfer pressure, and compartmentation efficiency -- Constantin Merezhkowsky and the endokaryotic hypothesis -- The diversity of mitochondrion-related organelles amongst eukaryotic microbes -- Mitosomes of parasitic protozoa: biology and evolutionary significance -- index.
  • 2006 Springer
    Civelli, Olivier; Zhou, Qun-Yong.
    Also available: Print – 2008
  • 2010
    Barnhart, Erin Lynn; Spudich, James A.; Straight, Aaron; Theriot, Julie A.
    Actin-based cell migration depends on the temporal and spatial integration of multiple force-generating systems. Polymerization of the actin network drives protrusion of the cell membrane, and the stretched membrane, in turn, exerts force on the actin network. Myosin contraction of the viscoelastic actin network is balanced by cell-substrate adhesion. The molecular components that contribute to each of these forces - actin-based protrusion, membrane tension, myosin-mediated contraction, and adhesion - have been extensively characterized, but the manner in which they are integrated in vivo to give rise to large-scale emergent properties such as cell shape and movement is not well understood. This thesis describes the role of cell-substrate adhesion in large-scale actin network organization and dynamic cell shape determination. Keratocytes exhibit several non-steady-state behaviors, including oscillatory retraction of the trailing edge, waves of protrusion at the leading edge, and symmetry breaking and motility initiation. I have shown that the mechanical and biochemical properties of adhesions contribute to each of these dynamic behaviors. Oscillatory retraction of the trailing edge emerges in fast-moving, fan-shaped keratocytes and I present evidence consistent with a mechanical model in which elastic coupling between the front of the cell and adhesions in the rear drives oscillations. In contrast, I have found that protrusion waves at the leading edge are likely the result of the biochemical, rather than mechanical, properties of adhesions. Traveling waves emerge at high adhesion strengths and adhesion maturation is required for waving. Mature adhesions have been shown previously to bind several components that contribute to leading edge protrusion, including VASP, a protein that promotes actin polymerization by preventing barbed end capping. Dynamic localization of VASP to the leading edge in waving keratocytes is consistent with a reaction-diffusion model in which global depletion of VASP by mature adhesion, in conjunction with local depletion by the polymerizing actin network, results in lateral propagation of protrusion waves. Finally, I present evidence that suggests that balance between cell-substrate adhesion and myosin contraction sets the mechanical instability threshold above which stationary, radially symmetric keratocytes break symmetry and begin to move. On the whole, the work described here represents significant progress towards elucidating the self-organization principles that underly whole cell migration.
  • 2007 ScienceDirect
    Häussinger, D.; Sies, H.
    Water compartments in cells / G. D. Fullerton ... [et al.] -- Yeast osmoregulation / S. Hohmann ... [et al.] -- Physiological analysis of bacterial mechanosensitive channels / I. R. Booth ... [et al.] -- Control of cell cycle in response to osmostress: Lessons from yeast / J. Clotet ... [et al.] -- Bacterial osmosensing transporters / J. M. Wood -- Plant gene networks in osmotic stress response: From genes to regulatory networks / L. S. Tran ... [et al.] -- Osmosensing by integrins in rat liver / F. Schliess ... [et al.] -- Hyperosmotic activation of the CD95 system / R. Reinehr ... [et al.] -- New approaches for determining apoptotic volume decrease in cells / C. D. Bortner ... [et al.] -- Transient receptor potential channels in mechanosensing and cell volume regulation / S. F. Pedersen ... [et al.] -- Cell volume regulatory ion channels in cell proliferation and cell death / F. Lang ... [et al.] -- Actin cytoskeleton architecture and signaling in osmosensing / E. A. Papakonstanti ... [et al.] -- Osmotic stress and DNA damage / N. I. Dmitrieva ... [et al.] -- Transcriptional activator tone-binding protein in cellular protection and differentiation / J. A. Kim ... [et al.] -- Desiccation response of mammalian cells: Anhydrosignaling / Z. Huang ... [et al.] -- Tonicity-regulated gene expression / J. D. Ferraris ... [et al.] -- Hyperosmotic induction of mitogen-activated protein kinase scaffolding / T. L. Hilder ... [et al.] -- Osmoregulation of bile formation / R. Kubitz ... [et al.] -- Osmosignaling and volume regulation in intestinal epithelial cells / C. H. Lim ... [et al.] -- Osmotic regulation of cellular glucose uptake / P. Gual ... [et al.] -- Effects of osmolytes on protein folding and aggregation in cells / Z. Ignatova ... [et al.] -- Simulations of macromolecules in protective and denaturing osmolytes: Properties of mixed solvent systems and their effects on water and protein structure and dynamics / D. A. Beck ... [et al.] -- Application of the transfer model to understand how naturally occurring osmolytes affect protein stability / M. Auton ... [et al.] -- Mechanisms of high salinity tolerance in plants / N. Tuteja -- Phenotype of the taurine transporter knockout mouse / U. Warskulat ... [et al.] -- Molecular basis of osmolyte effects on protein and metabolites / J. Rosgen -- Methods of changing biopolymer volume fraction and cytoplasmic solute concentrations for in vivo biophysical studies / M. C. Konopka ... [et al.] -- Characterization of plant aquaporins / R. Kaldenhoff ... [et al.].
    Also available: Print – 2007
  • 2006 MyiLibrary
    Attwood, Teresa K.; Cammack, Richard.
    Also available: Print – 2006
  • 2007 Springer
    Cooke, Marcus S.; Evans, Mark D.
    Oxidatively generated damage to cellular DNA : mechanistic aspects / Jean Cadet ... [et al.] -- Chlorination and nitration of DNA and nucleic acid components / Clare L. Hawkins ... [et al.] -- Prevention of the mutagenicity and cytotoxicity of oxidized purine nucleotides / Yusaku Nakabeppu ... [et al.] -- Nucleotide incision repair : an alternative and ubiquitous pathway to handle oxidative DNA damage / Sophie Couvé-Privat ... [et al.] -- OGG1 : from structural analysis to the knockout mouse / Arne Klungland, Jon K. Laerdahl, and Torbjörn Rognes -- Processing of 3'-end modified DNA strand breaks induced by oxidative damage / Jason L. Parsons, Emma Boswell, and Grigory L. Dianov -- Oxidative damage and promoter function / David Mitchell and Rita Ghosh -- Oxidative DNA damage and telomere shortening / Torsten Richter and Thomas von Zglinicki -- Oxidative damage and repair in the mitochondrial genome / Lene Juel Rasmussen and Keshav K. Singh -- The role of oxidative damage to nucleic acids in the pathogenesis of neurological disease / V. Prakash Reddy ... [et al.] -- Nucleic acid oxidation and the pathogenesis of cardiovascular diseases / Maria Grazia Andreassi -- Oxidative DNA damage and carcinogenesis / Ryszard Olinski, Marek Foksinski, and Barbara Tudek -- The physiological and pathological roles of oxidative damage to DNA in relation to life stage / Alberto Izzotti -- Analysis of 8-hydroxy-2'-deoxyguanosine as a marker of oxidatively damaged DNA in relation to carcinogenesis and aging / H. Kasai ... [et al.] -- Oxidatively damaged DNA and inflammation / Peter C. Dedon and Marita C. Barth -- The role of antioxidants in the prevention of oxidative damage to nucleic acids / Peter Muller and Steffen Loft.
  • v. 6-8, 10-22, 29-, 2001-02, 2004-06, 2012- CRCnetBase
    Cadenas, Enrique; Packer, Lester.
    Also available: Print – v. 15, 2005.
  • 2013 Springer Protocols
    Armstrong, D.; Bharali, Dhruba J.
    Nanoscale biosensor for detection of reactive oxygen species / Tarl W. Prow, Daniel Sundh, and Gerard A. Lutty -- Nanoparticles : toxicity, radicals, electron transfer, and antioxidants / Peter Kovacic and Ratnasamy Somanathan -- Encapsulation of antioxidants in gastrointestinal-resistant nanoparticulate carriers / Eliana B. Souto ... [et al.] -- Toxicity of novel nanosized rormulations used in medicine / Afaf El-Ansary ... [e tal.] -- Fullerenol nanoparticles : toxicity and antioxidant activity / Rade Injac, Matevz Prijatelj, and Borut Strukelj -- Hydrogen peroxide (H2O2) detection with nanoprobes for biological applications : a mini-review / Gwangseong Kim, Yong-Eun Koo Lee, and Raoul Kopelman -- Studying the oxidative stress paradigm in vitro : a theoretical and practical perspective / Martin J.D. Clift and Barbara Rothen-Rutishauser -- Evaluation of the effect of acute and subacute exposure to TiO2 nanoparticles on oxidative stress / Shichang Liu and Zhuo Yang -- Biomedical applications of nano-antioxidant / Geeta Watal ... [et al.] -- Lipid peroxidation due to in vitro and in vivo exposure of biological samples to nanoparticles / Anca Dinischiotu ... [et al.] -- Testing nanomaterial toxicity in unicellular eukaryotic algae and fish cell lines / Alexandra Kroll, Dana Kühnel, and Kristin Schirmer -- Gold nanoparticles and oxidative stress in the blue mussel, mytilus edulis / Sara Tedesco ... [et al.] -- Oxidative biomarkers to assess the nanoparticle-induced oxidative stress / Rama Narsimha Reddy Anreddy, Narsimha Reddy Yellu, and Krishna R. Devarakonda -- Vesicular antioxidants : role in age-related cerebral oxidative injury / Sibani Sarkar, Ardhendu Kumar Mandal, and Nirmalendu Das -- Methods for detection of oxidative stress and genotoxicity of engineered nanoparticles / Ashutosh Kumar, Vyom Sharma, and Alok Dhawan -- Size-dependent study of pulmonary responses to nano- sized iron and copper oxide nanoparticles / Rajiv Kumar and Dattatri K. Nagesha -- In vitro techniques to investigate the oxidative effects of quantum dots / Ridhima Juneja and Indrajit Roy -- Hybrid polymeric nanoparticles : potential candidate for ophthalmic delivery / Dhruba J. Bharali, Donald Armstrong, and Shaker A. Mousa -- Relevance of nanotechnology in modulating oxidative stress : an overview / Dhruba J. Bharali and Shaker A. Mousa.
  • 2013 Springer
    Jakob, Ursula; Reichmann, Dana.
    Many physiological conditions such as host defense or aging and pathological conditions such as neurodegenerative diseases, and diabetes are associated with the accumulation of high levels of reactive oxygen species and reactive nitrogen species. This generates a condition called oxidative stress. Low levels of reactive oxygen species, however, which are continuously produced during aerobic metabolism, function as important signaling molecules, setting the metabolic pace of cells and regulating processes ranging from gene expression to apoptosis. For this book we would like to recruit the experts in the field of redox chemistry, bioinformatics and proteomics, redox signaling and oxidative stress biology to discuss how organisms achieve the appropriate redox balance, the mechanisms that lead to oxidative stress conditions and the physiological consequences that contribute to aging and disease.
  • ScienceDirect
    Glazer, Alexander N.; Packer, Lester.
    Also available: Print – 1990
  • ScienceDirect
    Packer, Lester.
    Also available: Print – 1994
  • 2013 Springer
    Hainaut, Pierre; Olivier, Magali; Wiman, Klas.
    TP53: Coordinator of the Processes That Underlie the Hallmarks of Cancer / Pierre Hainaut -- The Inheritance of p53 / Lukasz F. Grochola, Jorge Zeron-Medina, Emmanouela Repapi, Alexander E. Finlayson and Ying Cai, et al. -- p53: Guardian of the Metabolome / Masha V. Poyurovsky and Carol Prives -- The p53 Family and Stem Cell Biology / Massimiliano Agostini, Alessandro Rufini, Edward T. W. Bampton, Francesca Bernassola and Gerry Melino, et al. -- Mutant p53-Driven Tumorigenesis / Tamara Terzian and Guillermina Lozano -- Humanised Mouse Models: Targeting the Murine p53 Locus with Human Sequences / Monica Hollstein and Yang Xu -- p53 Models for Mammary Carcinogenesis / Wolfgang Deppert and Genrich Tolstonog -- TP53 Somatic Mutations: Prognostic and Predictive Value in Human Cancers / Magali Olivier -- Assessing TP53 Status in Human Tumors: Lessons from Breast Cancer / Anita Langerød, Magali Olivier and Anne-Lise Børresen-Dale -- TP53 Germline Mutations: Genetics of Li-Fraumeni Syndrome / Doua Bakry and David Malkin -- Tp53 Gene Therapy for Cancer Treatment and Prevention / Robert E. Sobol, Yong-Song Guan, Long-Jiang Li, Wei-Wei Zhang and Zhaohui Peng, et al. -- Upstream Targets in the p53 Pathway / Anna R. McCarthy and Sonia Lain -- p53-Reactivating Molecules as Research Tools and Anticancer Drugs / Vera V. Grinkevich, Andreas Warnecke and Galina Selivanova -- Targeting Mutant p53 for Improved Cancer Therapy / Jinfeng Shen, Vladimir J. N. Bykov and Klas G. Wiman -- p53 Immunotherapy of Cancer / Hakim Echchannaoui and Matthias Theobald -- Designing p53 Trials: A Surgical Oncologists View / Daniela Kandioler, Sonja Kappel and Brigitte Wolf -- p53 in the Clinic: A Pathologist's View / Philippe Bertheau, Jean-François Fléjou, Yves Allory, Pascale Varlet and Sylvie Lantuejoul, et al. -- Genetic Counseling for TP53 Germline Mutations / Maria Isabel Waddington Achatz and Patricia Ashton-Prolla.
  • 2013 Springer Protocols
    Deb, Sumitra; Deb, Swati Palit.
    Detecting and quantifying p53 isoforms at mRNA level in cell lines and tissues / Marie P. Khoury ... [et al.] -- Detecting p53 isoforms at protein level / Virginie Marcel ... [et al.] -- Autophagy, senescence, and apoptosis / Rachel W. Goehe ... [et al.] -- p53 and cell cycle effects after DNA damage / Emir Senturk and James J. Manfredi -- p53 ubiquitination and proteasomal degradation / Ian M. Love, Dingding Shi, and Steven R. Grossman -- Identification of p53 in mitochondria / Angelina V. Vaseva and Ute M. Moll -- Identification of novel mutant p53 interacting proteins by proteomic analysis / Sumitra Deb and Paul R. Graves -- Identification of small molecules affecting p53-MDM2/MDMX interaction by fluorescence polarization / Qi Zhang and Hua Lu -- Determine the effect of p53 on chemosensitivity / Emir Senturk and James J. Manfredi -- Measurement of chemosensitivity and growth rate in p53 expressing cells / Mahesh Ramamoorthy ... [et al.] -- Mutant p53 in cell adhesion and motility / W. Andrew Yeudall, Katharine H. Wrighton, and Sumitra Deb -- Use of the DNA fiber spreading technique to detect the effects of mutant p53 on DNA replication / Rebecca A. Frum, Sumitra Deb, and Swati Palit Deb -- Generation of p53-deficient induced pluripotent stem cells from mouse embryo fibroblasts / Evguenia M. Alexandrova and Ute M. Moll -- p53 actions on microRNA expression and maturation pathway / Hiroshi I. Suzuki and Kohei Miyazono -- Isolation and characterization of murine multipotent lung stem cells / Venkat S. Gadepalli, Catherine Vaughan, and Raj R. Rao -- Generation of p53 knock-down cell lines / Catherine Vaughan, Swati Palit Deb, and Sumitra Deb -- ChIP for identification of p53 responsive DNA promoters / Jun-Ming Liao and Hua Lu -- ChIP-on-chip to identify mutant p53 targets / Frauke Goeman, Giulia Fontemaggi, and Giovanni Blandino -- ChIP sequencing to identify p53 targets / Catherine Vaughan, Brad Windle, and Sumitra Deb.
  • 2013 ScienceDirect
    Maruta, Hiroshi.
    1. Functional maturation of PAKs from unicellular to multicellular organisms / Masato Okada, Graham Cote, Ramesh K. Jha, and Hiroshi Maruta -- 2. Oncogenicity of PAKs and their substrates / Hong He and Hiroshi Maruta -- 3. Natural or synthetic therapeutics that block PAKs / Hiroshi Maruta, Shanta M. Messerli, and Ramesh K. Jha -- 4. PAK1-3 in infectious diseases / Hiroshi Maruta -- 5. PAK1 in brain diseases or disorders / Hiroshi Maruta and Shanta M. Messerli -- 6. PAK1 in Alzheimer's and Huntington's diseases / Qui-Lan Ma, Fusheng Yang, Sally A. Frautschy, and Greg M. Cole -- 7. PAK1 controls the lifespan / Sumino Yamase and Hiroshi Maruta -- 8. 3D structure and physiological regulation of PAKs / Stefan Knapp.
  • 2008 Springer
    Mackness, Bharti.
  • 2010 Springer
    Reddy, Srinivasa T.
    Also available: Print – 2010
  • 2013 CRCnetBASE
    Bustin, Stephen A.; Nolan, Tania.
    "A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution"--Provided by publisher.
  • 2011 ClinicalKey
    Van der Walle, Chris.
    The growing area of peptide and protein therapeutics research is of paramount importance to medical application and advancement. A needed reference for entry level researchers and researchers working in interdisciplinary. Covering cerebral delivery, pulmonary delivery, transdermal delivery, intestinal delivery, ocular delivery, parenteral delivery, and nasal delivery, this resource offers an overview of the main routes in therapeutics. Researchers across biochemistry, pharmaceutical, molecular biology, cell biology, immunology, chemistry and biotechnology fields will find this publication invaluable for peptide and protein laboratory research. Covers current and emerging routes for delivery of therapeutics with a focus on end application Covers Comparative Effectiveness Research Concise, accessible information from active researchers in academia and industry.
  • 2015 Springer Protocols
    Houen, Gunnar.
    Peptide Antibodies: Past, Present, and Future / Gunnar Houen -- Structure of natural and recombinant antibodies / Hui Ma and Richard O'Kennedy -- Prediction of antigenic B and T cell epitopes via energy decomposition analysis : description of the web-based prediction tool BEPPE / Claudio Peri ... [et al.] -- Prediction of antibody epitopes / Morten Nielsen and Paolo Marcatili -- Fmoc solid-phase peptide synthesis / Paul R. Hansen and Alberto Oddo -- Peptide-carrier conjugation / Paul R. Hansen -- Solid-phase peptide-carrier conjugation / Gunnar Houen and Dorthe T. Olsen -- Analysis of peptides and conjugates by amino acid analysis / Peter Højrup -- Characterization of synthetic peptides by mass spectrometry / Bala K. Prabhala ... [et al.] -- Interpretation of tandem mass spectrometry (MSMS) spectra for peptide analysis / Karin Hjernø and Peter Højrup -- Polyclonal Peptide Antisera / Tina H. Pihl, Kristin E. Illigen, and Gunnar Houen -- Production and screening of monoclonal peptide antibodies / Nicole Hartwig Trier ... [et al.] -- Production of epitope-specific antibodies by immunization with synthetic epitope peptide formulated with CpG-DNA- liposome complex without carriers / Dongbum Kim, Younghee Lee, and Hyung-Joo Kwon -- Thioredoxin-displayed multipeptide immunogens / Angelo Bolchi ... [et al.] -- Purification of natural and recombinant peptide antibodies by affinity chromatographic strategies / Hui Ma and Richard O'Kennedy -- Isolation of camelid single-domain antibodies against native proteins using recombinant multivalent peptide ligands / Norah A. Alturki ... [et al.] -- Generation of TCR-Like antibodies using phage display / Brian H. Santich ... [et al.] -- Structural characterization of peptide antibodies/ Anna Chailyan and Paolo Marcatili -- Automated high-throughput mapping of linear B-cell epitopes using a statistical analysis of high-density peptide microarray data / Thomas Østerbye and Søren Buus -- Characterization of peptide antibodies by epitope mapping using resin-bound and soluble peptides / Nicole Hartwig Trier -- Screening and characterization of linear B-cell epitopes by biotinylated peptide libraries / Ida Rosenkrands and Anja Olsen -- Bead-based peptide arrays for profiling the specificity of modification state-specific antibodies / Angela Filomena ... [et al.] -- Surface plasmon resonance method to evaluate anti-citrullinated protein/peptide antibody affinity to citrullinated peptides / Feliciana Real-Fernández ... [et al.] -- Specificity analysis of histone modification-specific antibodies or reading domains on histone peptide arrays / Goran Kungulovski ... [et al.] -- Prion-specific antibodies produced in wild-type mice / Peter M. H. Heegaard ... [et al.] -- Immunoblotting with peptide antibodies : differential immunoreactivities caused by certain amino acid substitutions in a short peptide and possible effects of differential refolding of the peptide on a nitrocellulose or PVDF membrane / Takenori Yamamoto ... [et al.] -- Immunocytochemical and immunohistochemical staining with peptide antibodies / Tina Friis ... [et al.] -- Designing B-cell epitopes for immunotherapy and subunit vaccines / Harinder Singh ... [et al.] -- Enterovirus-specific anti-peptide antibodies / Chit Laa Poh ... [et al.] -- Therapeutic HIV peptide vaccine / Anders Fomsgaard.
  • 2007 Springer Protocols
    Fields, Gregg B.
    HPLC analysis and purification of peptides / Colin T. Mant ... [et al.] -- Identification of proteins based on MS/MS spectra and location of posttranslational modifications / Kathryn L. Stone ... [et al.] -- Determination of phosphorylated and O-glycosylated sites by chemical targeting (CTID) at ambient temperature / Gary M. Hathaway -- Synthesis, biosynthesis, and characterization of transmembrane domains of a G protein-coupled receptor / Fred Naider -- Application of topologically constrained mini-proteins as ligands, substrates, and inhibitors / Janelle L. Lauer-Fields ... [et al.] -- Proteolytic profiling of the extracellular matrix degradome / Diane Baronas-Lowell ... [et al.] -- [Beta]-amyloid protein aggregation / Marcus A. Etienne ... [et al.] -- Radiometal-labeled somatostatin analogs for applications in cancer imaging and therapy / Jason S. Lewis and Carolyn J. Anderson -- Cell-penetrating proline-rich peptidomimetics / Josep Farrera-Sinfreu ... [et al.] -- Peptide-mediated targeting of liposomes to tumor cells / Evonne M. Rezler ... [et al.] -- Peptide-mediated delivery of nucleic acids into mammalian cells / Sébastien Deshayes ... [et al.] -- Broth microdilution antibacterial assay of peptides / Laszlo Otvos and Mare Cudic -- Depsipeptide synthesis / Maciej Stawikowski and Predrag Cudic.
  • 2015 Springer Protocols
    Derda, Ratmir.
    Design, synthesis, and application of OB2c combinatorial peptide and peptidomimetic libraries -- Synthesis of macrocyclic organo-peptide hybrids from ribosomal polypeptide precursors via cuAAC-/hydrazide-mediated cyclization -- Synthesis and screening of one-bead-one-compound cyclic peptide libraries -- Creating site-specific isopeptide linkages between proteins with the traceless staudinger ligation -- Macrocyclic templates for library synthesis of peptido-conjugates -- Synthesis and alkylation of aza-gly-pro building blocks of peptidomimetic libraries for developing prostaglandin f2alpha receptor modulators as therapeutics to inhibit preterm labor -- A fragment-based selection approach for the discovery of peptide macrocycles targeting protein kinases -- Peptide and peptide library cyclization via bromomethylbenzene derivatives -- Phage selection of bicyclic peptides based on two disulfide bridges -- De novo discovery of bioactive cyclic peptides using bacterial display and flow cytometry -- Chemical posttranslational modification of phage-displayed peptides -- Mapping protein-protein interactions with phage-displayed combinatorial peptide libraries and alanine scanning -- Identifying reactive peptides from phage-displayed libraries -- The best and the brightest: Exploiting tryptophan-sensitized tb(3+) luminescence to engineer lanthanide-binding tags -- Synthesis and cell-based screening of one-bead-one-compound peptide libraries -- Screening peptide array library for the identification of cancer cell-binding peptides -- Next-generation sequencing of phage-displayed peptide libraries -- Maleimide-based method for elaboration of cysteine-containing peptide phage libraries.
  • 2015 Springer Protocols
    Chiari, Marcella; Cretich, Marina.
    Peptide arrays on planar supports / Víctor Tapia Mancilla and Rudolf Volkmer -- High-throughput microarray incubations using multi-well chambers / Johannes Zerweck ... [et al.] -- Analysis of protein tyrosine kinase specificity using positional scanning peptide microarrays / Yang Deng and Benjamin E. Turk -- Secondary structure determination of peptides and proteins after immobilization / Stella H. North and Chris R. Taitt -- Peptides and anti-peptide antibodies for small and medium scale peptide and anti-peptide affinity microarrays : antigenic peptide selection, immobilization, and processing / Fan Zhang, Andrea Briones, and Mikhail Soloviev -- Low-cost peptide microarrays for mapping continuous antibody epitopes / Ryan McBride ... [et al.] -- Peptide microarray-based resonance light scattering assay for sensitively detecting intracellular kinase activity / Tao Li ... [et al.] -- Anomalous reflection of gold : a novel platform for biochips / Amir Syahir ... [et al.] -- High-throughput peptide screening on a bimodal imprinting chip through MS-SPRi integration / Weizhi Wang, Qiaojun Fang, and Zhiyuan Hu -- Analyzing peptide microarray data with the R pepStat package / Gregory Imholte, Renan Sauteraud, and Raphael Gottardo -- Chemoselective strategies to peptide and protein bioprobes immobilization on microarray surfaces / Alessandro Gori and Renato Longhi -- Manufacturing of peptide microarrays based on catalyst- free click chemistry / Denis Prim and Marc E. Pfeifer -- Clickable polymeric coating for oriented peptide immobilization / Laura Sola ... [et al.] -- Oriented peptide immobilization on microspheres / Lisa C. Shriver-Lake, George P. Anderson, and Chris R. Taitt -- Cell microarray format : a peptide release system using a photo-cleavable linker for cell toxicity and cell Uptake Analysis / Kenji Usui, Kin-ya Tomizaki, and Hisakazu Mihara -- Peptide microarrays for medical applications in autoimmunity, infection, and cancer / Carsten Grötzinger -- Synthetic peptide-based ELISA and ELISpot assay for identifying autoantibody epitopes / Judit Pozsgay ... [et al.] -- IgE and IgG4 epitope mapping of food allergens with a peptide microarray immunoassay / Javier Martínez-Botas and Belén de la Hoz -- IgE epitope mapping using peptide microarray immunoassay / Gustavo Gimenez, Sara Benedé, and Jing Lin -- Spot synthesis : an optimized microarray to detect IgE epitopes / Salvatore G. De-Simone, Paloma Napoleão-Pêgo, and Thatiane S. De-Simone -- Mapping of epitopes occurring in bovine αs1-casein variants by peptide microarray immunoassay / Maria Lisson and Georg Erhardt.
  • 2009 Springer Protocols
    Chiari, Marcella; Cretich, Marina.
    Exploring and profiling protein function with peptide arrays / V.E. Tapia, B. Ay and R. Volkmer -- Peptide arrays for enzyme profiling / A. Thiele, J. Zerweck and M. Schutkowski -- Using peptide array to identify binding motifs and interaction networks for modular domains / S.S. Li and C. Wu -- Molecular simulations of peptides: a useful tool for the development of new drugs and for the study of molecular recognition / M. Meli and G. Colombo -- Synthesis of peptide arrays using SPOT-technology and the CelluSpots-method / D.F. Winkler [and others] -- Rapid identification of linear protein domain binding motifs using peptide SPOT arrays / D.J. Briant [and others] -- Characterization of kinase target phosphorylation consensus motifs using peptide SPOT arrays / G.C. Leung [and others] -- CelluSpots: a reproducible means of making peptide arrays for the determination of SH2 domain binding specificity / C. Wu and S.S. Li -- High-density peptide microarrays for reliable identification of phosphorylation sites and upstream kinases / A. Thiele [and others] -- Epitope mapping of human chromogranin A by peptide microarrays / M. Cretich [and others] -- Antimicrobial peptide arrays for detection of inactivated biothreat agents / C.R. Taitt, S.H. North and N.V. Kulagina -- Mapping functional prion-prion protein interaction sites using prion protein based peptide-arrays / A. Rigter [and others] -- A designed peptide chip: protein fingerprinting technology with a dry peptide array and statistical data mining / K. Usui, K.Y. Tomizaki and H. Mihara -- Peptide microarrays on bisphenol A polycarbonate / V. Souplet, C. Roux and O. Melnyk -- Self-assembly of PNA-encoded peptides into microarrays / F. Debaene and N. Winssinger -- A novel combinatorial approach to high-density peptide arrays / M. Beyer [and others] -- Polypyrrole-peptide microarray for biomolecular interaction analysis by SPR imaging / M.B. Villiers [and others] -- The peptide microarray-based assay for kinase functionality and inhibition study / Z. Wang -- An advanced application of protein microarrays: cell-based assays for functional genomics / R. Carbone -- Profiling the autoantibody repertoire by screening phage-displayed human cDNA libraries / R. Di Niro [and others] -- Visualisation and pre-processing of peptide microarray data / M. Reilly and D. Valentini -- Web-based design of peptide microarrays using microPepArray Pro / T. Li [and others] -- Qualitative and quantitative analysis of peptide microarray binding experiments using SVM-PEPARRAY / G. Chen [and others] -- PASE: a web-based platform for peptide/protein microarray experiments / F. Pamelard [and others].
  • 2013 Springer Protocols
    Cudic, Predrag.