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  • Part 1. Cellular Aspects -- Elementary Cellular Processes Driven by Actin Assembly: Lamellipodia and Filopodia / J. Victor Small and Klemens Rottner -- Coupling Membrane Dynamics to Actin Polymerization / Shiro Suetsugu and Tadaomi Takenawa -- Endocytic Control of Actin-based Motility / Andrea Disanza, Emanuela Frittoli, Chiara Giuliani, Francesca Milanesi and Andrea Palamidessi, et al. -- Actin in Clathrin-Mediated Endocytosis / Marko Kaksonen -- Actin Cytoskeleton and the Dynamics of Immunological Synapse / Viveka Mayya and Michael L. Dustin -- Actin-based Motile Processes in Tumor Cell Invasion / Matthew Oser, Robert Eddy and John Condeelis -- Actin-based Chromosome Movements in Cell Division / Rong Li -- Roles for Actin Dynamics in Cell Movements During Development / Minna Roh-Johnson, Jessica Sullivan-Brown and Bob Goldstein -- Part 2. Molecular Aspects -- Regulation of the Cytoplasmic Actin Monomer Pool in Actin-based Motility / Pekka Lappalainen, Maarit Makkonen and Hongxia Zhao -- From Molecules to Movement: In Vitro Reconstitution of Self-Organized Actin-based Motile Processes / Marie-France Carlier and Dominique Pantaloni -- The WASP-Homology 2 Domain and Cytoskeleton Assembly / Roberto Dominguez -- Formin-Mediated Actin Assembly / David R. Kovar, Andrew J. Bestul, Yujie Li and Bonnie J. Scott -- Visualization of Individual Actin Filament Assembly / Emmanuèle Helfer -- Movement of Cargo in Bacterial Cytoplasm: Bacterial Actin Dynamics Drives Plasmid Segregation / Dyche Mullins -- Part 3. Physical Aspects -- Protrusive Forces Generated by Dendritic Actin Networks During Cell Crawling / Ovijit Chaudhuri and Daniel A. Fletcher -- Mathematical and Physical Modeling of Actin Dynamics in Motile Cells / Anders E. Carlsson and Alex Mogilner -- Force Production by Actin Assembly: Simplified Experimental Systems for a Thorough Modeling / C. Sykes, J. Prost and J.F. Joanny.
  • ADAR proteins : structure and catalytic mechanism / Rena A. Goodman, Mark R. Macbeth and Peter A. Beal -- ADAR proteins : double-stranded RNA and Z-DNA binding domains / Pierre Barraud and Frédéric H.-T. Allain -- Editing of neurotransmitter receptor and ion channel RNAs in the nervous system / Jennifer L. Hood and Ronald B. Emeson -- Modulation of microRNA expression and function by ADARs / Bjorn-Erik Wulff and Kazuko Nishikura -- Nuclear editing of mRNA 3'-UTRs / Ling-Ling Chen and Gordon G. Carmichael -- Control of ADAR1 editing of hepatitis delta virus RNAs / John L. Casey -- Bioinformatic approaches for identification of A-to-I editing sites / Eli Eisenberg -- ADARs : viruses and innate immunity / Charles E. Samuel -- Role of ADARs in mouse development / Carl R. Walkley, Brian Liddicoat and Jochen C. Hartner -- Regulation and functions of ADAR in Drosophila / Simona Paro, Xianghua Li, Mary A. O'Connell and Liam P. Keegan.
  • Introduction to adherens junctions: from molecular mechanisms to tissue development and disease -- How AJs evolved -- Evolution of the cadherin-catenin complex -- How cadherins and catenins interact to assemble AJs -- Three-dimensional structure of the cadherin-catenin complex -- Biophysics of cadherin adhesion -- Adherens junction assembly -- How AJs interface with other cellular machinery -- Cytoskeleton and classical cadherin adhesions -- Immunoglobulin superfamily receptors and adherens junctions -- Signaling from the adherens junction -- Adherens junction turnover: regulating adhesion through cadherin endocytosis, degradation, and recycling -- How AJs affect cell behaviour and multicellular development -- Adherens junctions during cell migration -- Adherens junctions and cadherins in Drosophilia development -- Adherens junctions in C. elegans embryonic morphogenesis -- Cadherin function during xenopus gastrulation -- Adherens junctions in mammalian development, homeostasis and disease: lessons from mice -- How AJs affect tissue homeostasis and disease -- Adherens junctions and stem cells -- Adherens junctions and cancer -- Adherens junctions and pathogen entry.
  • Adipokines 2012, CRCnetBASE
    1. Acylation stimulating protein (ASP) as an adipokine / Thea Scantlebury-Manning -- 2. Adiponectin as an adipokine / Zhikui Wei and G. William Wong -- 3. Adipsin as an adipokine / Yu-Feng Zhao and Chen Chen -- 4. Adrenomedullin as a cytokine / Alexis Elias Malavazos and Gianluca Iacobellis -- 5. Angiotensinogen as an adipokine / Miki Nagase and Toshiro Fujita -- 6. Apelin as an adipokine / Despina D. Briana and Ariadne Malamitsi-Puchner -- 7. C1q/TNF-related proteins (CTRPs) as adipokines / Jonathan M. Peterson and G. William Wong -- 8. Fasting-induced adipose factor/angiopoietin-like protein 4 as an adipokine / Sander Kersten -- Interleukins as adipokines / Kirsten Prüfer Stone -- Leptin as an adipokine: important definitions and applications for cancer research / Barbara Stadterman [and others] -- Omentin as an adipokine: role in health and disease / Anne M. Lenz and Frank Diamond -- Plasminogen activator inhibitor 1 (Pai-1) as an adipokine / Francis Agyemang-Yeboah -- Resistin as an adipokine / Zhikui Wei and G. William Wong -- Retinol-binding protein 4 as an adipokine / Elmar Aigner and Christian Datz -- TNF-α as an adipokine / Theron C. Gilliland, Jr. and G. William Wong -- Transforming growth factor as an adipokine / Yutaka Yata and Terumi Takahara -- Vaspin as an adipokine: a link between obesity and metabolic alterations / Peter Kovacs, Matthias Blüher, and Gabriela Aust -- Visfatin as an adipokine / Ariadne Malamitsi-Puchner and Despina D. Briana -- Thermal stress and adipokines expression / Umberto Bernabucci, Patrizia Morera, and Loredana Basiricò -- Central nervous system roles for adiponectin in neuroendocrine and autonomic function / Ted D. Hoyda, Willis K. Samson, and Alastair V. Ferguson -- The astroglial leptin receptors and obesity / Weihong Pan [and others] -- Adipokines and obesity / Alberto O. Chavez and Devjit Tripathy -- Nonalcoholic fatty liver disease and adipokines / Stergios A. Polyzos, Jannis Kountouras, and Christos Zavos -- Endocrine disruptors, adipokines, and the metabolic syndrome / Eric R. Hugo and Nira Ben-Jonathan -- Adipokines and the heart / Gianluca Iacobellis -- Adipokines as mediators of rheumatic disease / Deborah M. Levy and Earl D. Silverman -- Adipokines and allergy / Giorgio Ciprandi [and others] -- Adipokines and sleep disorders / Kapil Dhawan [and others] -- Adipokines in children and adolescents and implications for disease / HuiLing Lu and Katherine Cianflone -- Role of leptin in immune cell development and innate immunity / Pamela J. Fraker and Afia Naaz -- Visfatin and hypocaloric diets / D.A. de Luis and R. Aller -- Apelin in energy metabolism / Isabelle Castan-Laurell and Philippe Valet -- Adiponectin deficiency / Wayne Bond Lau [and others] -- Adiponectin enhances inflammation in rheumatoid synovial fibroblasts and chondrocytes / Natsuko Kusunoki, Kanako Kitahara, and Shinichi Kawai -- Resistin, imflammation and seminal fluid / Uwe Paasch [and others] -- Resistin in amniotic fluid / Shali Mazaki-Tovi [and others].
  • "This volume explores the use of mass spectrometry for biomedical applications. Chapters focus on specific therapeutic areas such as oncology, infectious disease and psychiatry. Additional chapters focus on methodology as well as new technologies and instrumentation. This volume provides readers with a comprehensive and informative manual that will allow them to appreciate mass spectrometry and proteomic research but also to initiate and improve their own work. Thus the book acts as a technical guide but also a conceptual guide to the newest information in this exciting field. Mass spectrometry is the central tool used in proteomic research today and is rapidly becoming indispensable to the biomedical scientist. With the completion of the human genome project and the genomic revolution, the proteomic revolution has followed closely behind. Understanding the human proteome has become critical to basic and clinical biomedical research and holds the promise of providing comprehensive understanding of human physiological processes. In addition, proteomics and mass spectrometry are bringing unprecedented biomarker discovery and are helping to personalize medicine"--Publisher's description.
  • Advances in dyslipidemia 2014, Future Med
    Advances in dyslipidemia / Wilbert S. Aronow -- Atherosclerosis : dyslipidemia, inflammation and lipoapoptosis / Fleur M. van der Valk, Dominik M. Schulte, Willem J.M. Mulder & Erik S.G. Stroes -- Familial hypercholesterolemia : bridging and minding the gap in healthcare / Jing Pang, Alistair W. Vickery & Gerald F. Watts -- PCSK9 inhibition : drug development for low-density lipoprotein lowering / Geetha Mukerji, Rawand M. Abdin & Dominic S. Ng -- Novel therapies for dyslipidemia / Anthony S. Wierzbicki -- The highs and lows of high-density lipoprotein / Daniel Soffer & Marisa Schoen -- Do we need to treat patients with heart failure with statins? / Sadip Pant, Guru S. Gurumurthy, Abhishek Deshmukh & Jawahar L. Mehta -- Management of dyslipidemia in patients with nonalcoholic fatty liver disease / Konstantinos Tziomalos, Vasilios G. Athyros & Asterios Karagiannis -- Dyslipidemia : current management and the challenge of residual cardiovascular risk reduction / Aaron Rockoff, Vaijinath S. Kamanna & Moti L. Kashyap -- Index.
  • Improvement of thermostable enzyme with sugar metabolic activity by targeted mutagenesis -- Glycoside hydrolases for modification of glycosylation in polyphenolic antioxidants -- On the enzyme specificity for the synthesis of prebiotic galacto-oligosaccharides -- Microbial mannanases: properties and applications -- Enzyme Synergy for Enhanced Degradation of Lignocellulosic Waste -- Manganese peroxidase: molecular diversity, heterologous expression and applications -- Advance Techniques in Enzyme Research -- Regulatory motif identification in biological sequences: An overview of computational methodologies -- Structural, Molecular and Functional Aspects of Chitin deacetylase -- Role of enzymes and proteins in plant -- microbe interaction : A study of M. oryzae vs Rice -- Industrial enzyme applications in biorefineries for starchy materials.
  • Section 1. Overview of enzymes. Introduction to enzymes ; The limits for life define the limits for enzymes ; Enzyme kinetics ; Properties and evolution of allosteric enzymes ; Kinetics of allosteric enzymes -- Section 2. K-type enzymes. Hemoglobin ; Glycogen phosphorylase ; Phosphofructokinase ; Ribonucleotide reductase ; Hexokinase -- Section 3. V-type enzymes. Introduction to V-type enzymes ; G proteins ; Protein kinases.
  • This up-to-date book presents basic concepts and recent advances in amino acid biochemistry and nutrition, providing comprehensive coverage of developments over the past 45 years. It includes information on absorption, metabolism, and excretion of amino acids while addressing protein quality and requirements. The text discusses metabolic pathways of amino acids in relation to health and disease in various organisms, as well as species differences in metabolism of amino acids. The author also presents a critical analysis of amino acid supplementation to humans and animals.
  • Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.
  • Engineered antibodies currently represent over 30 per cent of biopharmaceuticals in clinical trials and their total worldwide sales continue to increase significantly. The importance of antibody applications is reflected in their increasing clinical and industrial applications as well as in the progression of established and emerging production strategies. This volume provides detailed coverage of the generation, optimization, characterization, production and applications of antibody. It provides the necessary theoretical background and description of methods for the expression of antibody in.
  • APC proteins 2009, Springer
    APC and [beta]-catenin degradation -- Nuclear APC -- APC in cell migration -- The APC-EB1 interaction -- The role of APC in mitosis and in chromosome instability -- Role of APC and its binding partners in regulating microtubules in mitosis -- The adenomatous polyposis coli tumor -- Suppressor and Wnt signaling in the regulation of apoptosis -- APC and its modifiers in colon cancer -- Tissue-specific tumour suppression by APC -- Extra-colonic manifestations of familial adenomatous polyposis coli.
  • A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites. The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages. Most importantly, it is fundamentally quantitative with a wide dynamic range. The output of the analysis is a numerical result that can range over several orders of magnitude. Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures. Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment. This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins.
  • Aquaporins 2009, Springer
  • This volume describes our current understanding of the biological role of visual and non-visual arrestins in different cells and tissues, focusing on the mechanisms of arrestin-mediated regulation of GPCRs and non-receptor signaling proteins in health and disease. The book covers wide range of arrestin functions, emphasizing therapeutic potential of targeting arrestin interactions with individual partners.
  • Artificial enzymes 2005, Wiley
    Artificial enzymes / Ronald Breslow -- Vitamin B6 enzyme models / Lei Liu and Ronald Breslow -- Evolution of synthetic polymers with enzyme-like catalytic activities / Irving M. Klotz and Junghun Suh -- Mimicking enzymes with antibodies / Donald Hilvert -- Protein-based artificial enzymes / Ben Duckworth and Mark D. Distefano -- Artificial hydrolytic metalloenzymes / Jik Chin and Hae-Jo Kim -- Artificial restriction enzymes as tools for future molecular biology and biotechnology / Yoji Yamamoto and Makoto Komiyama.
  • Homeostatic functions of BCL-2 proteins beyond apoptosis / Nika N. Danial, Alfredo Gimenez-Cassina, and Daniel Tondera -- Alternative functions of the BCL-2 protein family at the endoplasmic reticulum / Diego Rojas-Rivera ... [et al.] -- BH3-only proteins and their effects on cancer / Thanh-Trang Vo and Anthony Letai -- Endoplasmic reticulum stress and BCL-2 family members / Ross T. Weston and Hamsa Puthalakath -- Targeting survival pathways in lymphoma / Luca Paoluzzi and Owen A. O'Connor -- The interplay between BCL-2 family proteins and mitochondrial morphology in the regulation of apoptosis / Maria Eugenia Soriano and Luca Scorrano -- Noncanonical functions of BCL-2 proteins in the nervous system / Heather M. Lamb and J. Marie Hardwick -- Mitochondria on guard : role of mitochondrial fusion and fission in the regulation of apoptosis / Mariusz Karbowski.
  • Big picture medical biochemistry 2012, AccessMedicine
    Section I. The Basic Molecules of Life -- 1. Amino acids and proteins -- 2. Carbohydrates -- 3. Lipids -- 4. Nucleosides, nucleotides -- Section II. Functional biochemistry -- 5. Enzymes and amino acid metabolism -- 6. Carbohydrate metabolism -- 7. Lipid metabolism -- 8. Membranes -- 9. DNA/RNA function and protein synthesis -- Section III. Applied biochemistry -- 10. Metabolism and vitamins/minerals / Maria L. Valencik and Cynthia C. Mastick -- 11. The Digestive system / Kshama Jaiswal -- 12. Muscles and cell motility / Darren Campbell -- 13. Connective tissue and bone / Jacques Kerr -- 14. Blood / Matthew Porteus -- 15. The Immune system / Eric L. Greidinger, Leonard M. Miller -- 16. The Cardiovascular system / Ralph Shohet, John A. Burns -- 17. The respiratory system / Howard Huang -- 18. The Urinary system / Armando J. Lorenzo -- 19. The Nervous system / Kathryn Beck-Yoo -- 20. The Reproductive system / Catrina Bubier -- Section IV. Appendices -- Appendix I. Biochemical Basis of Diseases -- Appendix II. Biochemical Methods -- Appendix III. Organic Chemistry Primer.
  • Bio-Nanoimaging: Protein Misfolding & Aggregation provides a unique introduction to both novel and established nanoimaging techniques for visualization and characterization of misfolded and aggregated protein species. The book is divided into three sections covering: - Nanotechnology and nanoimaging technology, including cryoelectron microscopy of beta(2)-microglobulin, studying amyloidogensis by FRET; and scanning tunneling microscopy of protein deposits - Polymorphisms of protein misfolded and aggregated species, including fibrillar polymorphism, amyloid-like protofibrils, and insulin olig.
  • Bioactive egg compounds 2007, Springer
  • "Presenting data from human studies and trials, along with recent research findings, this work summarizes the applications, and benefits of bioactive peptides used to mitigate major metabolic derangements arising from chronic illnesses and resulting in unwanted weight loss. Recent studies show bioactive peptides to enhance the body's antioxidant status, antisepsis capacity, immune function, anti-inflammatory capacity, mineral absorption, and appetite. This book covers general principles, such as host response, quality factors, protein economics, and muscle loss. It includes case studies on ageing, AIDS, COPD, diabetes, inflammatory bowel disease, kidney failure, and tuberculosis."--Provided by publisher.
  • Angiogenesis plays a key role in human physiology and pathophysiology. While necessary for tissue growth and development, uncontrolled angiogenesis plays a role in the progression of certain tumors as well as atherosclerosis. Lack of angiogenesis may be the basis of myocardial ischemia and stroke. Knowledge on the mechanisms of angiogenesis is growing very rapidly, and may lead to important drugs for therapy of a variety of clinical disorders. Experts from around the world have contributed a vast array of data on different aspects of angiogenesis in this volume edited by Professors Mehta and Dhalla. This information will be of immense help to basic scientists, clinicians and those involved in drug development.
  • Consists of critical reviews and original research papers from the 2014 International Symposium on the "Biochemical Role of Eukaryotic Cell Surface Macromolecules". Topics covered include: · neurochemical and biochemical analysis of cell surface glycoconjugates · membrane skeletal organization · GPCRs and other receptors · biophysical approaches to study membrane interactions · glycoconjugate metabolism · dysregulation · molecular mechanisms involved in cell-cell and cell-matrix interaction · glycans in infectious and neurological diseases · cancer and glycosyltransferases as drug targets.
  • Glycomics: An Overview of the Complex Glycocode / Garima Gupta and Avadhesha Surolia -- Structural Glycomic Approaches to Molecular Recognition Events on Cell Surfaces / Koichi Kato -- ISCSM2011 Chondroitin Sulfate E-type Structure at Tumor Cell Surface Is Involved in Experimental Metastasis / Kazuyuki Sugahara and Shuji Mizumoto -- Chondroitin Sulfate-Specific Novel Hydrolase in Human / Shuhei Yamada -- Identification of Endothelial Cell Surface Carbohydrate-Binding Receptors by Carbohydrate Ligand Mimicry Peptides / Michiko N. Fukuda -- Lysosomal Enzyme Sorting Receptors - Where Did They First Appear in the Animal Kingdom? / Suryanarayanaraju Vegiraju, Suresh Koduru and Siva Kumar Nadimpalli -- Endoplasmic Reticulum-Targeted Bcl-2 Inhibitable Mitochondrial Fragmentation Initiates ER Stress-Induced Cell Death / B. C. Bhavya, Deepa Indira, Mahendra Seervi, Jeena Joseph and Praveen K. Sobhan, et al. -- Interactions Between Caveolin-1 and Sphingolipids, and Their Functional Relevance / Sandro Sonnino, Simona Prioni, Vanna Chigorno and Alessandro Prinetti -- Cell Membrane Repair Pathway Involves Sensing of Dynamics of Caveolae and Caspase-1 / Saumya S. Srivastava and M. V. Krishnasastry -- Angiogenic Response of Endothelial Cells to Fibronectin / V. B. S. Kumar, R. I. Viji, M. S. Kiran and Perumana R. Sudhakaran -- Lactosylceramide Synthase as a Therapeutic Target to Mitigate Multiple Human Diseases in Animal Models / Subroto Chatterjee and Nezar Alsaeedi -- Advanced FRET Methodologies: Protein-Lipid Selectivity Detection and Quantification / Fábio Fernandes, Manuel Prieto and Luís M. S. Loura -- Mechanism of GPCR-Directed Autoantibodies in Diseases / Hamiyet Unal, Rajaganapathi Jagannathan and Sadashiva S. Karnik -- Role of Membrane Cholesterol in Leishmanial Infection / Amitabha Chattopadhyay and Md. Jafurulla -- How Intact Is the Basement Membrane? Role of MMPs / S. Asha Nair, Sankar Jagadeeshan, Ramachandran Indu, Perumana R. Sudhakaran and M. R. Pillai -- Apoptosis of Breast Cancer Cells: Modulation of Genes for Glycoconjugate Biosynthesis and Targeted Drug Delivery / Subhash Basu, Rui Ma, Joseph R. Moskal, Manju Basu and Sipra Banerjee -- Altered Expression of Sialidases in Human Cancer / Taeko Miyagi, K. Takahashi, S. Moriya, K. Hata and K. Yamamoto, et al. -- Poly-ADP-Ribosylation of Vascular Endothelial Growth Factor and Its Implications on Angiogenesis / S. Binu, S. J. Soumya, V. B. S. Kumar and Perumana R. Sudhakaran -- Sphingolipid-Binding Domain in the Serotonin1A Receptor / Amitabha Chattopadhyay, Yamuna Devi Paila, Sandeep Shrivastava, Shrish Tiwari and Pushpendra Singh, et al. -- Withanolide D, Carrying the Baton of Indian Rasayana Herb as a Lead Candidate of Antileukemic Agent in Modern Medicine / Susmita Mondal, Saptarshi Roy, Rita Maity, Asish Mallick and Rajender Sangwan, et al. -- Engineered Glucose to Generate a Spectroscopic Probe for Studying Carbohydrate Biology / Ashish Tripathi, Vibha Singh, K. G. Aishwarya, Gopala Krishna Aradhyam and Srinivas Hotha -- Changes in Sialic Acid Content of Jelly Coat in Pesticide-Exposed Frog Eggs and Their Influence on Fertilization / H. P. Gurushankara, S. V. Krishnamurthy and V. Vasudev.
  • Abbreviations and acronyms -- Glossary of terms useful in biochemistry and molecular biology and related disciplines -- Chemicals commonly used in biochemistry and molecular biology and their properties -- A listing of log P values, water solubility, and molecular weight for some selected chemicals -- Protease inhibitors and protease inhibitor cocktails -- List of buffers -- Organic name reactions useful in biochemistry and molecular biology.
  • Functional roles of lipids in membranes / William Dowhan, Mikhail Bogdanov, and Eugenia Mileykovskaya -- Lipid modifications of proteins / Anant K. Menon -- Fatty acid and phospholipid metabolism in prokaryotes / Charles O. Rock -- Lipid metabolism in plants / Katherine M. Schmid and John B. Ohlrogge -- Oxidation of fatty acids in eukaryotes / Horst Schulz -- Fatty acid synthesis in eukaryotes / Hei Sook Sul and Stuart Smith -- Fatty acid desaturation and chain elongation in mammals / Makoto Miyazaki and James N. Ntambi -- Phospholipid biosynthesis in eukaryotes / Dennis E. Vance and Jean E. Vance -- Ether-linked lipids and their bioactive species / Thomas M. McIntyre, Fred Snyder, and Gopal K. Marathe -- Lipid metabolism in adipose tissue / Ann V. Hertzel ... [et al.] -- Phospholipases / David C. Wilton -- The eicosanoids : cyclooxygenase, lipoxygenase, and epoxygenase pathways / William L. Smith and Robert C. Murphy -- Sphingolipids / Alfred H. Merrill, Jr. -- Cholesterol biosynthesis / Laura Liscum -- Metabolism and function of bile acids / Luis B. Agellon -- Lipid assembly into cell membranes / Dennis R. Voelker -- Lipoprotein structure / Ana Jonas and Michael C. Phillips -- Assembly and secretion of triacylglycerol-rich lipoproteins / Jean E. Vance and Khosrow Adeli -- Dynamics of lipoprotein transport in the circulatory system / Christopher J. Fielding and Phoebe E. Fielding -- Lipoprotein receptors / Wolfgang J. Schneider -- Lipids and atherosclerosis / Ira Tabas.
  • A role for vitamin A in living organisms has been known throughout human history. In the last 100 years, the biochemical nature of vitamin A and its active derivative, retinoic acid, its physiological impact on growth processes and the essential details of its mechanism of action have been revealed by investigations carried out by researchers using vertebrate and more recently invertebrate models to study a multiplicity of processes and conditions, encompassing embryogenesis, postnatal development to old age. A wealth of intercellular interactions, intracellular signaling systems and molecular mechanisms have been described and the overall conclusion is that retinoic acid is essential for life. This book series, with chapters authored by experts in every aspect of this complex field, unifies the knowledge base and mechanisms currently known in detailed, engaging, well-illustrated, focused chapters that synthesize information for each specific area. In view of the recent explosion in this field, it is timely to publish a contemporary, comprehensive, book series recapitulating the most exciting developments in the field and covering fundamental research in molecular mechanisms of vitamin A action, its role in physiology, development and continued well-being and the potential of vitamin A derivatives and synthetic mimetics to serve as therapeutic treatments for cancers and other debilitating human diseases. VOLUME I: Here, we present the first volume of a multi-volume series on Retinoic Acid Signaling that will cover all aspects of this broad and diverse field. One aim of Volume I is to present a compilation of topics related to the biochemistry of nuclear retinoic acid receptors, from their architecture when bound to DNA and associated with their coregulators to their ability to regulate target gene transcription. A second aim is to provide insight into recent advances that have been made in identifying novel targets and non-genomic effects of retinoic acid. Volume I is divided into ten chapters contributed by prominent experts in their respective fields. Each chapter starts with the history of the area of research. Then, the key findings that contributed to development of the field are described, followed by a detailed look at key findings and progress that are being made in current, ongoing research. Each chapter is concluded with a discussion of the relevance of the research and a perspective on missing pieces and lingering gaps that the author recommends will be important in defining future directions in vitamin A research.
  • The regulation of gene expression -- The regulation of enzyme activity -- Structure and function of signal pathways -- Signaling by nuclear receptors -- G protein-coupled signal transmission pathways -- Intracellular messenger substance : second messengers -- Ser/Thr-specific protein kinases and protein phosphates -- Signal transmission via transmembrane receptors with tyrosine-specific protein kinase activity -- Signal transmission via ras proteins -- Intracellular signal transduction : the protein cascades of the MAP kinase pathways -- Membrane receptors with associated tyrosine kinase activity -- Other receptor classes -- Regulation of the cell cycle -- Malfunction of signaling pathways and tumorigenesis : oncogenes and tumor suppressor genes -- Apoptosis.
  • Biochemistry. 3rd ed. 2011, ClinicalKey
    Carbohydrates, lipids, and amino acids : metabolic fuels and biosynthetic precursors -- Proteins and enzymes -- Membrane biochemistry and signal transduction -- Nutrition -- Generation of energy from dietary fuels -- Carbohydrate metabolism -- Lipid metabolism -- Nitrogen metabolism -- Integration of metabolism -- Nucleotide synthesis and metabolism -- Organization, synthesis, and repair of DNA -- Gene expression -- DNA technology -- Common laboratory values.
  • Biochemistry. 5th ed. 2002, NCBI Bookshelf
  • Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. *Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab *Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates *Features full color illustrations *Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented.
  • Theory of organelle biogenesis : a historical perspective / Barbara M. Mullock and J. Paul Luzio -- Protein coats as mediators of intracellular sorting and organelle biogenesis / Chris Mullins -- The role of proteins and lipids in organelle biogenesis in the secretory pathway / Thomas F.J. Martin -- Endoplasmic reticulum biogenesis : proliferation and differentiation / Erik Snapp -- The golgi apparatus : structure, function and cellular dynamics / Nihal Altan-Bonnet and Jennifer Lippincott-Schwartz -- Lysosome biogenesis and dynamics / Diane McVey Ward, Shelly L. Shiflett and Jerry Kaplan -- Nucleogenesis / Sui Huang -- Mitochondrial biogenesis / Danielle Leuenberger, Sean P. Curran and Carla M. Koehler -- The biogenesis and cell biology of peroxisomes in human health and disease / Stanley R. Terlecky and Paul A. Walton.
  • Clinical Bioinformatics in Human Proteomics Research / Duojiao Wu, Haihao Li -- Proteomics Defines Protein Interaction Network of Signaling Pathways / Shitao Li -- Protein Function Microarrays: Design, Use and Bioinformatic Analysis in Cancer Biomarker Discovery and Quantitation / Jessica Duarte, Jean-Michel Serufuri, Nicola Mulder -- Proteomics and Cancer Research / Elena Lopez Villar -- Toward Development of Novel Peptide-Based Cancer Therapeutics: Computational Design and Experimental Evaluation / Elena Pirogova, Taghrid Istivan -- Advances in Proteomic Methods / Xianyin Lai -- Clinical and Biomedical Mass Spectrometry: New Frontiers in Drug Developments and Diagnosis / Ákos Végvári, Melinda Rezeli -- Disease Biomarkers: Modelling MR Spectroscopy and Clinical Applications / Luis Martí-Bonmati -- Processing of Mass Spectrometry Data in Clinical Applications / Dario Di Silvestre, Pietro Brunetti, Pier Luigi Mauri -- Bioinformatics Approach for Finding Target Protein in Infectious Disease / Hemant Ritturaj Kushwaha, Indira Ghosh -- Identification of Network Biomarkers for Cancer Diagnosis / Jiajia Chen, Luonan Chen -- Software Development for Quantitative Proteomics Using Stable Isotope Labeling / Xin Huang, Shi-Jian Ding -- Clinical Translation of Protein Biomarkers Integrated with Bioinformatics / Xu Yang, Juanjuan Zhou, Chaoqin Du -- Proteomic Approaches for Urine Biomarker Discovery in Bladder Cancer / Ming-Hui Yang, Yu-Chang Tyan -- Antibody Microarrays and Multiplexing / Jerry Zhou, Larissa Belov, Nicola Armstrong -- Proteomics in Anaesthesia and Intensive Care Medicine / Ornella Piazza, Giuseppe De Benedictis.
  • ch. 1. Inorganic chemistry essentials -- ch. 2. Biochemistry fundamentals -- ch. 3. Instrumental and computer-based methods -- ch. 4. Iron containing oxygen carriers and their synthetic models -- ch. 5. Copper Enzymes -- ch. 6. The Enzyme nitrogenase -- ch. 7. Metals in medicine.
  • ch. 1. Inorganic chemistry essentials -- ch. 2. Biochemistry fundamentals -- ch. 3. Instrumental methods -- ch. 4. Computer hardware, software, and computational chemistry methods -- ch. 5 Group I and II metals in biological systems : homeostasis and group I biomolecules -- ch. 6. Group I and II metals in biological systems : group II -- ch. 7. Iron-containing proteins and enzymes.
  • Biological DNA Sensor defines the meaning of DNA sensing pathways and demonstrates the importance of the innate immune responses induced by double stranded DNA (dsDNA) through its influencing functions in disease pathology and immune activity of adjuvants for vaccines. Though discussed in specific subsections of existing books, dsDNA and its immunogenic properties has never received the complete treatment given in this book. Biological DNA Sensor approaches the impact of dsDNA's immunogenicity on disease and vaccinology holistically. It paints a complete and concise picture on the topic so you can understand this area of study and make more informed choices for your respective research needs. Chapters are authored by researchers who are renowned for their research focus, ensuring that this book provides the most complete views on the topics. Multi-authored by a distinguished panel of world-class expertsIdeal source of information for those wanting to learn about DNA sensingProvides in-depth explanations of DNA sensing pathways and the innate immune system, bridging the gap between them
  • Biological mass spectrometry 2005, ScienceDirect
  • Biology of serpins. 1st ed. 2011, ScienceDirect
  • This book fills in a gap in the NO literature. Recent progress in the field of NO-biology shows that NO is generated within distinct cell compartments, including specific plasma membrane regions, mitochondria, chloroplasts, peroxisomes, the Golgi-complex and intracellular membrane systems. NO synthesis plays specific roles in these compartments and, in turn, cell organelles also control intracellular NO levels. This monograph focuses on the roles played by the subcellular NO-signaling microdomains in the prokaryote-, fungus-, plant- and animal cells and shows how NO behaves as an intracellular signal in distinct cellular environments. This monograph also provides a summary of our knowledge on how NO synthesis came through evolution to be associated with organelles and subcellular compartments. Promotes the novel ideas that some functions of NO and its associations with subcellular units have been conserved during the evolution of the cell. A special chapter is dedicated to the biomedical relevance of subcellular NO synthesis, and this chapter also discusses the evidence that altered compartmentalization of NO-producing enzymes causes disease.
  • When quantum mechanics was first proposed a century ago, nobody could have anticipated how deeply it would affect our lives. Today, we are connected and powered through devices whose existence is predicated on the basic principles of this strange physics. Not even the biological sciences have escaped its reach. As scientists query the deepest mysteries of the living world, the physical scales probed and the types of questions asked are increasingly blurring the lines between biology and physics. The hybrid field of biophysics represents the new frontier of the 21st century. The ribosome has been at the heart of three Nobel Prizes. Understanding its essential nature and how it interacts with other proteins and nucleic acids to control protein synthesis has been one of the central foundations in our understanding of the biology at the molecular level. With the advent of atomic scale structures, methods to visualize and separate individual molecules, and the computational power to model the complex interactions of over a million atoms at once, our understanding of how gene expression is controlled at the level of protein translation is now deeply ensconced in the biophysical realm. This book provides a premier resource to a wide audience, whether it be the general reader seeking a broad view of the field, a clinician interested in the role of protein translation in human disease, the bench researcher looking for state-of-the-art technologies, or computational scientists involved in cutting edge molecular modeling.
  • pt. B, 2009 ScienceDirect
  • "Discussing the role of plasma proteins in current biotechnology, this book describes the protein composition of human plasma, the fractionation of plasma to obtain therapeutic proteins, and the analysis of these products. It delineates the path from plasma products to recombinant products, and highlights products from albumin, intravenous immunoglobins, and coagulation. It offers a comprehensive review of current techniques for the analysis of proteins including electrophoresis, chromatography, spectrophotometry, and mass spectrometry as well as updates not published since 1975"--Provided by publisher.
  • Biothermodynamics. Part A-D. 1st ed. pt. A-D, 2009-11., ScienceDirect
    pt. B, 2009 ScienceDirect
    pt. C, 2011 ScienceDirect
    pt. D, 2011 ScienceDirect
  • The administration of intravenous fluids is one of the most common and important therapeutic practices in the treatment of surgical, medical and critically ill patients. The international literature accordingly contains a vast number of works on fluid management, yet there is still confusion as to the best options in the various situations encountered in clinical practice.The purpose of this volume is to help the decision-making process by comparing different solution properties describing their indications, mechanisms of action and side-effects according to physiologic body water distribution, electrolytic and acid-base balance, and to clarify which products available on the market represent the best choice in different circumstances. The book opens by discussing in detail the concepts central to a sound understanding of abnormalities in fluid and electrolyte homeostasis and the effect of intravenous fluid administration. In the second part of the monograph, these concepts are used to explain the advantages and disadvantages of solutions available on the market in different clinical settings. Body Fluid Management: From Physiology to Therapy will serve as an invaluable decision-making guide, including for those who are not experts in the subject.
  • Overview of resistance to systemic therapy in patients with breast cancer / Ana Maria Gonzalez-Angulo, Flavia Morales-Vasquez and Gabriel N. Hortobagyi -- Roles of multidrug resistance genes in breast cancer chemoresistance / M. Tien Kuo -- Therapy-induced apoptosis in primary tumors / David J. McConkey -- Cell cycle deregulation in breast cancer: insurmountable chemoresistance or achilles' heel? / Laura Lambert and Khandan Keyomarsi -- p53, BRCA1 and breast cancer chemoresistance / Kimberly A. Scata and Wafik S. El-Deiry -- Integrin-mediated adhesion: tipping the balance between chemosensitivity and chemoresistance / Mary M. Zutter -- Insulin-like growth factors and breast cancer therapy / Xianke Zeng and Douglas Yee -- EGF receptor in breast cancer chemoresistance / Robert B. Dickson and T.B. Deb -- Molecular mechanisms of ErbB2-mediated breast cancer chemoresistance / Ming Tan and Dihua Yu -- Estrogen receptors in resistance to hormone therapy / Matthew H. Herynk and Suzanne A.W. Fuqua -- Novel approaches for chemosensitization of breast cancer cells: the E1A story / Yong Liao, Dihua Yu and Mien-Chie Hung.
  • Calcium signaling 2012, Springer
    "This volume ... [explores] topics ranging from the fundamental aspects of calcium signaling to its varied clinical implications. It presents comprehensive discussion of cutting-edge research alongside detailed analysis of critical issues, at the same time as setting out testable hypotheses that point the way to future scientific endeavors. The contributions feature material on theoretical and methodological topics as well as related subjects including mathematical modeling and simulations. They examine calcium signaling in a host of contexts, from mammalian cells to oocytes, zebrafish and plants. With much of interest to newcomers to the field as well as seasoned experts, this new publication is both wide-ranging and authoritative." --Excerpt from publisher's description.
  • Ryanodine and Inositol Trisphosphate Receptors/Ca2+ Release Channels in Airway Smooth Muscle Cells / Lin Mei, Yun-Min Zheng, and Yong-Xiao Wang -- Kv7 (KCNQ) Potassium Channels and L-type Calcium Channels in the Regulation of Airway Diameter / Kenneth L. Byron, Lioubov I. Brueggemann, Priyanka P. Kakad, and Jennifer M. Haick -- Transient Receptor Potential and Orai Channels in Airway Smooth Muscle Cells / Jun-Hua Xiao, Yong-Xiao Wang, and Yun-Min Zheng -- Large-conductance calcium-activated potassium channels / Hiroaki Kume -- Calcium-activated Chloride Channels / George Gallos and Charles W. Emala Sr. -- Local Calcium Signaling in Airway Smooth Muscle Cells / Quing-Hua Liu, Carlo Savoia, Yong-Xiao Wang -- Regulation of Airway Smooth Muscle Contraction by Ca2+ Signaling: Physiology Revealed by Microscopy Studies of Lung Slices / Michael J. Sanderson -- Temporal Aspects of Ca2+ Signaling in Airway Myocytes / Etienne Roux -- Mechanisms Underlying Ca2+ Store Refilling in Airway Smooth Muscle / Charlotte K. Billington, Ian P. Hall, and Carl P. Nelson -- Novel Mechanisms in Ca2+-homeostasis and Internal Store Refilling of Airway Smooth Muscle / Luke J. Janssen -- The Role of Mitochondria in Calcium Regulation in Airway Smooth Muscle / Philippe Delmotte, Li Jia, and Gary C. Sieck -- Role of Caveolae in the Airway / Christina M. Pabelick, Brij B. Singh, and Y.S. Prakash -- CD38 : Cyclic ADP-ribose-mediated Calcium Signaling in Airway Myocytes /Deepak A. Deshpande, Alonso Guedes, Mythili Dileepan, Timothy F. Walseth, and Mathur S. Kannan -- The Pathways and Signaling Cross-talk with Oxidant in Calcium Influx in Airway Smooth Muscle Cells / Lei Cai and Quinghua Hu -- Role of RhoA/Rho-kinase and Calcium Sensitivity in Airway Smooth Muscle Functions / Satoru Ito -- Role of Integrins in the Regulation of Calcium Signaling / Thai Tran and Chun Ming Teoh -- Sex Steroid Signaling in the Airway / Y.S. Prakash, Venkatachalem Sathish, and Elizabeth A. Townsend -- Regulation of Contractility in Immature Airway Smooth Muscle / Y.S. Prakash, Christina M. Pabelick, and Richard J. Martin -- Mathematical Modeling of Calcium Dynamics in Airway Smooth Muscle Cells / James Sneyd, Pengxing Cao, Xiahui Tan, and Michael J. Sanderson -- Effects of Inflammatory Cytokines on Ca2+ Homeostasis in Airway Smooth Muscle Cells / Hisako Matsumoto -- Ca2+ Signaling and P2 Receptors in Airway Smooth Muscle / Luis M. Montaño, Edgar Flores-Soto, and Carlos Barajas-López -- Calcium Signaling in Airway Smooth Muscle Remodeling / Tengyao Song, Yun-min Zheng, and Yong-Xiao Wang -- Regulation of Intracellular Calcium by Bitter Taste Receptors on Airway Smooth Muscle / Deepak A. Deshpande and Stephen B. Liggett -- Modulation of Airway Smooth Muscle Contractile Function by TNFa and IL-13 and Airway Hyper-responsiveness in Asthma / Yassine Amrani -- Airway Smooth Muscle Malfunction in COPD / Yunchao Su.
  • Calcium-binding protein protocols v. 1-2, 2002., Springer Protocols
    v. 2, 2002 Springer Protocols
    v. 1. Reviews and case studies -- v. 2. Methods and techniques.
  • Proteomics technologies -- Cell signaling proteomics -- Tumor proteomics -- Bioinformatics and regulatory aspects of proteomics.
  • ch. 1. Synthetic methodologies / Chikako Saotome, Osamu Kanie -- ch. 2. Complex carbohydrate synthesis / Makoto Kiso, Hideharu Ishida, Hiromune Ando -- ch. 3. The chemistry of sialic acid / Geert-Jan Boons, Alexei V. Demchenko -- ch. 4. Solid-phase oligosaccharide synthesis / Peter H. Seeberger -- ch. 5. Solution and polymer-supported synthesis of carbohydrates / Shin-Ichiro Nishimura -- ch. 6. Enzymatic synthesis of oligosaccharides / Jianbo Zhang, Jun Shao, Prezemk Kowal, Peng George Wang -- ch. 7. Glycopeptides and glycoproteins : synthetic chemistry and biology / Oliver Seitz -- ch. 8. Synthesis of complex carbohydrates : Everninomicin 13,384-1 / K. C. Nicolaou, Helen J. Mitchell, Scott A. Snyder -- ch. 9. Chemical synthesis of asparagine-linked glycoprotein oligosaccharides : recent examples / Yukishige Ito, Ichiro Matsuo -- ch. 10. Chemistry and biochemistry of asparagine-linked protein glycosylation / Barbara Imperiali, Vincent W.-F. Tai -- ch. 11. Conformational analysis of C-glycosides and related compounds : programming conformational profiles of C- and O-glycosides / Peter G. Goekjian, Alexander Wei, Yoshito Kishi -- ch. 12. Synthetic lipid A antagonists for sepsis treatment / William J. Christ, Lynn D. Hawkins, Michael D. Lewis, Yoshito Kishi -- ch. 13. Polysialic acid vaccines / Harold J. Jennings -- ch. 14. Synthetic carbohydrate-based vaccines / Stacy J. Keding, Samuel J. Danishefsky -- ch. 15. Chemistry, biochemistry, and pharmaceutical potentials of glycosaminoglycans and related saccharides / Tasneem Islam, Robert J. Linhardt -- ch. 16. A new generation of antithrombotics based on synthetic oligosaccharides / Maurice Petitou, Jean-Marc Herbert -- ch. 17. Sequencing of oligosaccharides and glycoproteins / Stuart M. Haslam, Kay-Hooi Khoo, Anne Dell -- ch. 18. Preparation of heterocyclic 2-deoxystreptamine aminoglycoside analogues and characterization of their interaction with RNAs by use of electrospray ionization mass spectrometry / Richard H. Griffey, Steven A. Hofstadler, Eric E. Swayze -- ch. 19. Glycosylation analysis of a recombinant P-selectin antagonist by high-pH anion-exchange chromatography with pulsed electrochemical detection (HPAEC/PED) / Mark R. Hardy, Richard J. Cornell -- ch. 20. Analytical techniques for the characterization and sequencing of glycosaminoglycans / Ram Sasisekharan, Zachary Shriver, Mallik Sundaram, Ganesh Venkataraman -- ch. 21. Thermodynamic models of the multivalency effect / Pavel I. Kitov, David R. Bundle -- ch. 22. Synthetic multivalent carbohydrate ligands as effectors or inhibitors of biological processes / Laura L. Kiessling, Jason K. Pontrello, Michael C. Schuster -- ch. 23. Glycosyltransferase inhibitors / Karl-Heinz Jung, Richard R. Schmidt -- ch. 24. RNA-aminoglycoside interactions / Haim Weizman, Yitzhak Tor -- ch. 25. Glycosylated natural products / Jon S. Thorson, Thomas Vogt -- ch. 26. Novel enzymatic mechanisms in the biosynthesis of unusual sugars / Alexander Wong, Xuemei He, Hung-Wen Liu -- ch. 27. Neoglycolipids : identification of functional carbohydrate epitopes / Ten Feizi, Alexander M. Lawson, Wengang Chai -- ch. 28. A preamble to aglycone reconstruction for membrane-presented glycolipid mimics / Murugesapillai Mylvaganam, Clifford A. Lingwood -- ch. 29. Small molecule inhibitors of the sulfotransferases / Dawn E. Verdugo, Lars C. Pedersen, Carolyn R. Bertozzi -- ch. 30. Carbohydrate-based treatment of cancer metastasis / Reiji Kannagi -- ch. 31. N-acetylneuraminic acid derivatives and mimetics as anti-influenza agents / Robin Thomson, Mark Von Itzstein -- ch. 32. Modified and modifying sugars as a new tool for the development of therapeutic agents : the biochemically engineered N-acyl side chain of sialic acid : biological implications and possible uses in medicine / Rüdiger Horstkorte, Oliver T. Keppler, Werner Reutter -- ch.33 . Modified and modifying sugars as a new tool for the development of therapeutic agents : glycosidated phospholipids as a new type of antiproliferative agents / Kerstin Danker, Annette Fischer, Werner Reutter -- ch. 34. Glycoside primers and inhibitors of glycosylation / Jillian R. Brown, Mark M. Fuster, Jeffrey D. Esko -- ch. 35. Carbohydrate-based drug discovery in the battle against bacterial infections : new opportunities arising from programmable one-pot oligosaccharide synthesis / Thomas K. Ritter, Prof. Dr. Chi-Huey Wong.
  • 1. Basics in carbohydrate chemistry / Heinrich Hühnerfuss -- 2. Glycoconjugates : a brief overview / Peter Grunwald -- 3. Oligosaccharides and glycoconjugates in recognition processes / Thisbe K. Lindhorst -- 4. Glycoside hydrolases / Motomitsu Kitaoka -- 5. Disaccharide phosphorylases : mechanistic diversity and application in the glycosciences / Christiane Luley-Goedl and Bernd Nidetzky -- 6. Enzymatic and chemoenzymatic synthesis of nucleotide sugars : novel enzymes, novel substrates, novel products, and novel routes / Leonie Engels and Lothar Elling -- 7. Iteratively acting glycosyltransferases / Marta Luzhetska and Andreas Bechthold -- 8. Bacterial glycosyltransferases involved in molecular mimicry of mammalian glycans / Warren Wakarchuk -- 9. Sulfotransferases and sulfatases : sulfate modification of carbohydrates / Eli Chapman and Sarah R. Hanson -- 10. Glycosylation in health and disease / Peter Grunwald -- 11. Sialic acid derivatives, analogues, and mimetics as biological probes and inhibitors of sialic acid recognizing proteins / Joe Tiralongo and Thomas Haselhorst -- 12. Enzymes of the carbohydrate metabolism and catabolism for chemoenzymatic syntheses of complex oligosaccharides / Julian Thimm and Joachim Thiem -- 13. From gene to product : tailor-made oligosaccharides and polysaccharides by enzyme and substrate engineering / Jürgen Seibel -- 14. Synthesis and modification of carbohydrates via metabolic pathway engineering in microorganisms / Xian-wei Liu ... [et al.] -- 15. Metabolic pathway engineering for hyaluronic acid production / Esteban Marcellin, Wendy Y. Chen, and Lars K. Nielsen -- 16. Microbial rhamnolipids / Markus M. Müller ... [et al.] -- 17. Chitin-converting enzymes / Karin Moss ... [et al.] -- 18. Linear and cyclic oligosaccharides / Hajime Taniguchi -- 19. Fungal degradation of plant oligo- and polysaccharides / Ronald P. de Vries ... [et al.] -- 20. Bacterial strategies for plant cell degradation and their genomic information / Yutaka Tamaru and Roy H. Doi -- 21. Design of efficient multienzymatic reactions for cellulosic biomass processing / Anne S. Meyer.
  • The study of carbonic anhydrase has spanned multiple generations of scientists. Carbonic anhydrase was first discovered in 1932 by Meldrum and Roughton. Inhibition by sulfanilamide was shown in 1940 by Mann and Keilin. Even Hans Krebs contributed to early studies with a paper in 1948 showing the relationship of 25 different sulfonamides to CA inhibition. It was he who pointed out the importance of both the charged and uncharged character of these compounds for physiological experiments. The field of study that focuses on carbonic anhydrase (CA) has exploded in recent years with the identification.
  • The processes of adaptation and maladaptation play an important role in the pathogeny of serious cardiovascular diseases, such as hypertension, valvular diseases, congenital heart disease, myocardial infarction and different cardiomyopathies as well as during adaptation to exercise and high altitude hypoxia. This volume incorporates the rapidly developing basic and clinically relevant information on adaptive mechanisms, thereby contributing to the better understanding of possible prevention and therapy of life-threatening cardiovascular diseases. The first section of this volume focuses on developmental aspects of cardiac adaptation, including chapters on comparative and molecular aspects of cardiac development, prenatal and postnatal developments, coronary vascular development, and ontogenetic adaptation to hypoxia, as well as cardiac and arterial adaptation during aging. The second section is devoted to cardiac adaptations to overload on the heart, centered around the mechanisms of cardiac hypertrophy due to pressure overload, volume overload, exercise, gender difference, high altitude, and different pathological situations. The third section of this volume highlights the roles of sympathetic nervous system with respect to [alpha]-adrenoceptor and [beta]-adrenoceptor mechanisms in the development of cardiac hypertrophy. Cardiac Adaptations will be of great value to cardiovascular investigators, who will find this book highly useful in their cardiovascular studies for finding solutions in diverse pathological conditions; it will also appeal to students, fellows, scientists, and clinicians interested in cardiovascular abnormalities.
  • Hepatic retinoid metabolism : what is known and what is not / Jason J. Yuen, Jian Zhang Yang and William S. Blaner -- Metabolism of vitamin A in white adipose tissue and obesity / Shanmugam M. Jeyakumar ... [et al.] -- Retinoids in the treatment of psoriasis / Neil Borkar, Alan Menter -- Age-related macular degeneration and carotenoids / Nilesh Kalariya, Werner G. Siems, Frederik van Kuijk -- Lutein, zeaxanthin and vision across the lifespan / Billy R. Hammond, Jr. -- Assuring vitamin A adequacy to prevent eye and ear disorders / Tripper Sauer, Susan Emmett, Keith P. West, Jr. -- Disturbed accumulation and abnormal distribution of macular pigment in retinal disorders / Tos TJM Berendschot, Peter Charbel Issa, Thomas Theelen -- Retinol and otitis media / Akeem Olawale Lasisi -- Carotenoids and mutagenesis / P. M. Eckl ... [et al.] -- Carotenoids and vitamin A in lung cancer prevention / Anita Ratnasari Iskandar, Xiang-Dong Wang -- Is the effect of [Beta]-carotene on prostate cancer cells dependent on their androgen sensitivity? / Joanna Dulinska-Litewka ... [et al.] -- Carotenoid intake and supplementation in cancer : pro and con / W.G. Siems ... [et al.] -- Vitamin A in lung development and function / Hans-Konrad Biesalski -- Vitamin A and carotenoids in lung diseases / Olaf Sommerburg, Werner Siems -- Chemopreventive effects of astaxanthin on inflammatory bowel disease and inflammation-related colon carcinogenesis / Masashi Hosokawa, Yumiko Yasui -- Carotenoids in laboratory medicine / Ingolf Schimke, Michael Vogeser -- Supply of vitamin A in developing countries / Klaus Kraemer -- Vitamin A intakes are below recommendations in a significant proportion of the population in the western world / Hans-Konrad Biesalski, Barbara Trösch, Petra Weber -- Critical appraisal of vitamin A supplementation program in India / Dechenla Tshering Bhutia, Saskia De Pee -- Consequences of common genetic variations on SS-carotene cleavage for vitamin A supply / Georg Lietz, Anthony Oxley, Christine Boesch-Saadatmandi.
  • "Carotenoids are of great interest due to their essential biological functions in both plants and animals. However, the properties and functions of carotenoids in natural systems are surprisingly complex. With an emphasis on the chemical aspects of these compounds, Carotenoids: Physical, Chemical, and Biological Functions and Properties presents a broad overview and recent developments with respect to understanding carotenoid structure, electronic and photochemical properties, and the use of novel analytical methods in the detection and characterization of carotenoids and their actions. The text also explores LC/MS and LC/MS/MS techniques as well as new applications of PCR and molecular biology methodologies"--Book jacket.
  • Through the use of extended graphical abstracts and some traditional text-only abstracts this collection provides, a record of and roadmap to, the research presented at The Tenth International Catecholamine Symposium (XICS) held in September of 2012. Organized around ten general themes, each is introduced by a short overview identifying interesting research programs, results and potential areas of growth. The collection is a roadmap to key research and future opportunities for new catecholamine research programs and will be of interest to neuroscientists and clinical neurologists interested in understanding the current and future state of catecholamine research..
  • Lipid rafts, caveolae, and GPI linked proteins / Valerie L. Reeves, Candice M. Thomas, and Eric J. Smart -- Caveolae and the regulation of endocytosis / Anna L. Kiss -- Caveolin : role in cell signaling / Cecile Boscher and Ivan Robert Nabi -- Regulation of ENOS in caveolae / Chieko Mineo and Philip W. Shaul -- Recent developments in the interactions between caveolin and pathogens / Fabiana S. Machado ... [et al.] -- Caveolin and breast cancer : a new clinical perspective / Isabelle Mercier and Michael P. Lisanti -- Caveolin and prostate cancer progression / Michael R. Freeman, Wei Yang, and Dolores Di Vizio -- Caveolins and caveolae, roles in insulin signalling, and diabetes / Peter Strolfors -- Atherosclerosis, caveolae, and caveolin / Stephanos Pavlides ... [et al.] -- Caveolins and heart diseases / Mathivadhani Panneerselvam, Hemal H. Patel, and David M. Roth -- Caveolins and lung function / Nikolaos A. Maniatis ... [et al.].
  • 1. A Recent Breakthrough in the CCN Field: Functional Interactions Between CCN2 and CCN3 are Uncovered / Bernard Perbal -- 2. Report on the Fifth International Workshop on the CCN Family of Genes / A. E. Irvine, B. Perbal, and H. Yeger -- 3. Asking the Right Questions: What Can the Structure of the CCN Protein Domains Tell Us? / Kenneth P. Holbourn, Bernard Perbal, and K. Ravi Acharya -- 4. Nucleophosmin/B23: A Multifunctional Regulator that Determines the Fate of CCN2 mRNA / Satoshi Kubota, Yoshiki Mukudai, Harumi Kawaki, Seiji Kondo, Takanori Eguchi, Kumi Sumiyoshi, Toshihiro Ohgawara, Tsuyoshi Shimo, and Masaharu Takigawa -- 5. The CCN Genes as the “Master” Regulators of Angiogenesis, Vasculogenesis, Fibrogenesis and Cell Differentiation/Fate Specification in Mechanical Force-Driven Developmental Processes and Pathological Events / Mary Hanna and Brahim Chaqour -- 6. A Monoclonal Antibody Approach to CCN5 Domain Analysis / Lan Wei, Frank McKeon, Joshua W. Russo, Joan Lemire, and John Castellot -- 7. Matricellular Protein CCN2 Produced by Tubular Epithelial Cells Plays a Pivotal Role in Renal Fibrogenesis / Hirokazu Okada, Tsutomu Inoue, and Hiromichi Suzuki -- 8. Cooperative Regulation of Cell Proliferation and Differentiation by CCN2 and CCN3 / Masaharu Takigawa, Harumi Kawaki, Satoshi Kubota, Karen M. Lyons, and Bernard Perbal -- 9. The Role of CCN3 in Mesenchymal Stem Cells / Ken-Ichi Katsube -- 10. Role of Connective Tissue Growth Factor in Cardiac Fibrosis / Daiji Kawanami, Saptarsi M. Haldar, and Mukesh K. Jain -- 11. Gene Expression of CCN Family Members in Young and Aged Human Skin In Vivo / Taihao Quan, Sharon Shin, Zhaoping Qin, and Gary J. Fisher -- 12. Global Expression Profiling Reveals a Role for CTGF/CCN2 in Lactogenic Differentiation of Mouse Mammary Epithelial Cells / Weihan Wang, Cynthia Jose, Nicholas Kenney, Bethanie Morrison, and Mary Lou Cutler -- 13. CCN3 (NOV): A Negative Regulator of CCN2 (CTGF) Activity and an Endogenous Inhibitor of Fibrosis in Experimental Diabetic Nephropathy / Bruce. L. Riser, Feridoon Najmabadi, Bernard Perbal, Jo Ann Rambow, Melisa L. Riser, Ernest Sukowski, Herman Yeger, Sarah C. Riser, and Darryl R. Peterson -- 14. Inhibitors of Connective Tissue Growth Factor (CCN2)-Mediated Fibrogenesis: Underlying Mechanisms and Prospects for Anti-fibrotic Therapy / David R. Brigstock -- 15. CCN3 Promotes Melanoma Progression by Regulating Integrin Expression, Adhesion and Apoptosis Induced by Cytotoxic Drugs / Viviana Vallacchi, Maria Daniotti, Annamaria De Filippo, Licia Rivoltini, Bernard Perbal, and Monica Rodolfo -- 16. CCN3: A NOVel Growth Factor in Leukaemia / Lynn McCallum and Alexandra E Irvine -- 17. Prognostic Relevance of CCN3 in Bone Sarcomas / Bernard Perbal, Noureddine Lazar, Diana Zambelli, Monia Zuntini, Massimo Serra, Jose Antonio Lopez-Guerrero, Antonio Llombart-Bosch, Piero Picci, and Katia Scotlandi -- 18. CCN6 Regulates Breast Cancer Growth and Invasion Through Modulation of IGF Signaling and Epithelial to Mesenchymal Transition / Anupama Pal, Wei Huang, and Celina G. Kleer -- 19. Novel Transcriptional Regulation of CCN2/CTGF by Nuclear Translocation of MMP3 / Takanori Eguchi, Satoshi Kubota, Kazumi Kawata, Yoshiki Mukudai, Junji Uehara, Toshihiro Ohgawara, Soichiro Ibaragi, Akira Sasaki, Takuo Kuboki, and Masaharu Takigawa -- Fifth International Workshop on the CCN Family of Genes: Abstracts and Posters October 18-22, 2008.
  • Cell stress proteins 2007, Springer
    Surveys the knowledge concerning the expression and function of stress proteins in different organisms, ranging from prokaryotes to humans. This book provides an overview of the diversity and complex evolutionary history of cell stress proteins and describes their function and expression in different eukaryote models.
  • Cellulases. 1st ed. 2012, ScienceDirect
    Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume in the Methods in Enzymology series comprehensively covers this topic.
  • Chaperones 2006, Springer
  • This Brief provides a concise review of chaperonopathies, i.e., diseases in which molecular chaperones play an etiologic-pathogenic role. Introductory chapters deal with the chaperoning system and chaperoning teams and networks, HSP-chaperone subpopulations, the locations and functions of chaperones, and chaperone genes in humans. Other chapters present the chaperonopathies in general, including their molecular features and mechanistic classification into by defect, excess, or mistake. Subsequent chapters discuss the chaperonopathies in more detail, focusing on their distinctive characteristic.
  • At the forefront of life sciences today is the emerging discipline of chembiomolecular science. This new term describes the integration of the frontier fields of chemical biology, chemistry, and pharmacology. Chembiomolecular science aims to elucidate new biological mechanisms as potential drug targets and enhance the creation of new drug therapies. This book comprises the proceedings of the Uehara Memorial Foundation Symposium 2011, which focused on the most recent advances in chembiomolecular science made by leading experts in the field. The book is divided into three main topics. The first is the chemical approach to understanding complex biological systems on a molecular level using chemical compounds as a probe. The second describes the biological approach used to develop new lead drug compounds. The third focuses on the biological system that serves as the potential drug target, the beginning step in the process of developing new drugs. Replete with the latest research, the book will draw the attention of all scientists interested in the synergies between chemistry and biology to elucidate life on a molecular level and to promote drug discovery. Ultimately, the book helps promote the understanding of biological functions at the molecular level and create new pharmaceuticals that can contribute to improving human health.
  • "Natural products play crucial roles in modern drug development and constitute a prolific source of novel lead compounds or pharmacophores for ongoing drug discovery programs. Chemistry and Pharmacology of Naturally Occurring Bioactive Compounds presents cutting-edge research in the chemistry of bioactive natural products and demonstrates how natural product research continues to make significant contributions in the discovery and development of new medicinal entities."--Page [4] of cover.
  • Chemokine receptors 2007, Springer
  • pt. B ScienceDirect
    pt. C ScienceDirect
  • Chromatin and disease 2007, Springer
  • Circadian clock 2010, Springer
  • pt. B ScienceDirect
    Two new volumes of Methods in Enzymology continue the legacy of this premier serial with quality chapters authored by leaders in the field. Circadian Rhythms and Biological Clocks Part A and Part B is an exceptional resource for anybody interested in the general area of circadian rhythms. As key elements of timekeeping are conserved in organisms across the phylogenetic tree, and our understanding of circadian biology has benefited tremendously from work done in many species, the volume provides a wide range of assays for different biological systems. Protocols are provided to assess clock function, entrainment of the clock to stimuli such as light and food, and output rhythms of behavior and physiology. This volume also delves into the impact of circadian disruption on human health. Contributions are from leaders in the field who have made major discoveries using the methods presented here.
  • The clinical biochemistry laboratory -- The use of the laboratory -- The interpretation of results -- Point of care testing -- Reference intervals -- Fluid and electrolyte balance : Concepts and vocabulary -- Water and sodium balance -- Hyponatraemia : pathophysiology -- Hyponatraemia : assessment and management -- Hypernatraemia -- Hyperkalaemia -- Hypokalaemia -- Intravenous fluid therapy -- Investigation of renal function (1) -- Investigation of renal function (2) -- Urinalysis -- Proteinuria -- Acute renal failure -- Chronic renal failure -- Acid-base : concepts and vocabulary -- Metabolic acid-base disorders -- Respiratory and mixed acid-base disorders -- Oxygen transport -- Acid-base disorders : diagnosis and management -- Proteins and enzymes -- Immunoglobulins -- Myocardial infarction -- Liver function tests -- Jaundice -- Liver disease -- Glucose metabolism and diabetes mellitus -- Diagnosis and monitoring of diabetes mellitus -- Diabetic ketoacidosis -- Hypoglycaemia -- Calcium regulation and hypocalcaemia -- Hypercalcaemia -- Phosphate and magnesium -- Bone disease -- Osteoporosis -- Endocrine control -- Dynamic function tests -- Pituitary function -- Growth disorders and acromegaly -- Thyroid pathophysiology -- Hypothyroidism -- Hyperthyroidism -- Adrenocortical pathophysiology -- Hypofunction of the adrenal cortex -- Hyperfunction of the adrenal cortex -- Gonadal function -- Subfertility -- Nutritional assessment -- Nutritional support -- Parenteral nutrition -- The metabolic response to injury -- Gastrointestinal disorders -- Iron -- Zinc and copper -- Therapeutic drug monitoring -- Toxicology -- Metal poisoning -- Alcohol -- Coma -- Ascites and pleural fluid -- Cerebrospinal and other body fluids -- Lipoprotein metabolism -- Clinical disorders of lipid metabolism -- Hypertension -- Cancer and its consequences -- Tumour markers -- Multiple endocrine neoplasia -- Hyperuricaemia -- Myopathy -- Biochemistry in the elderly -- Fetal monitoring and prenatal diagnosis -- Pregnancy -- Antenatal screening -- Screening the newborn for disease -- Paediatric biochemistry -- Inborn errors of metabolism -- Selected inherited disorders -- Case history comments -- Web resources.
  • Human plasma lipoprotein metabolism -- Regulation and clearance of apolipoprotein B-containing lipoproteins -- Absorption and excretion of intestinal cholesterol and other sterols -- High-density lipoprotein metabolism -- Lipoproteins : mechanisms for atherogenesis and progression of atherothrombotic disease -- Impact of rare and common genetic variants on lipoprotein metabolism -- Lipoprotein oxidation : mechanisms and biotheranostic applications -- Cholesterol : concentration, ratio, and particle number -- High-density lipoprotein cholesterol and triglycerides in coronary heart disease risk assessment -- Lipoprotein(a) -- Clinical evaluation for genetic and secondary causes of dyslipidemia -- Use of high-sensitivity c-reactive protein for risk assessment -- Role of lipoprotein-associated phospholipase A2 in vascular disease -- Emerging assays -- Imaging atherosclerosis for risk stratification by cardiac computed tomography or carotid ultrasound -- Overview of general approach to management of elevated low-density lipoprotein cholesterol and mixed dyslipidemia, high triglycerides, and low high-density lipoprotein cholesterol -- Treatment guidelines overview -- Dietary patterns for the prevention and treatment of cardiovascular disease -- Exercise and lipids -- Weight loss -- Statins -- Bile acid sequestrants -- Cholesterol absorption inhibitors -- Niacin (nicotinic acid) -- Fibrates -- Omega-3 fatty acids -- Combination therapy for dyslipidemia -- Nutraceuticals and functional foods for cholesterol reduction -- Evolving targets of therapy : cholesteryl ester transfer protein inhibition -- Evolving targets of therapy : proprotein convertase subtilisin/kexin 9 inhibition -- Evolving targets of therapy : inflammation as a method to predict and prevent cardiovascular events -- Invasive imaging modalities and atherosclerosis : intravascular ultrasound -- Noninvasive imaging modalities and atherosclerosis : the role of ultrasound -- Noninvasive imaging modalities and atherosclerosis : the role of magnetic resonance imaging and positron emission tomography imaging -- Special patient populations : diabetes and metabolic syndrome -- Special patient populations : women and elderly -- Special patient populations : children and adolescents -- Special patient populations : familial hypercholesterolemia and other severe hypercholesterolemias -- Special patient populations : acute coronary syndromes -- Special patient populations : transplant recipients -- Special patient populations : chronic kidney disease -- Special patient populations : lipid abnormalities in high-risk ethnic groups -- Special patient populations : human immunodeficiency virus patients -- Therapeutic targeting of high-density lipoprotein metabolism.
  • Basics -- Chemistry -- Physical Chemistry -- Biomolecules -- Carbohydrates -- Lipids -- Amino acids -- Peptides and proteins -- Nucleotides and nucleic acids -- Metabolism -- Enzymes -- Metabolic pathways -- Energy metabolism -- Carbohydrate metabolism -- Lipid metabolism -- Protein metabolism -- Nucleotide metabolism -- Porphyrin metabolism -- Cell organelles -- Basics -- Cytoskeleton -- Nucleus -- Mitochondria -- Membranes -- ER and Golgi apparatus -- Lysosomes -- Peroxisomes -- Molecular Genetics -- Overview -- Genome -- Chromatin -- Gennetic code -- Genetic engineering -- Tissues and organs -- Digestive system -- Blood -- Immune system -- Liver -- Adipose tissue -- Kidney -- Muscle -- Connective tissue -- Brain and sensory organs -- Integration of metabolism -- Nutrition -- Nutrients -- Vitamins -- Signaling systems -- Signal transduction -- Hormone systems -- Lipophilic signaling substances -- Hydrophilic signaling substances -- Growth and development -- Appendix.
  • This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers computational prediction RNA structure and dynamics, including such topics as computational modeling of RNA secondary and tertiary structures, riboswitch dynamics, and ion-RNA, ligand-RNA and DNA-RNA interactions.Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers computational methods and applications in RNA structure and dynamics.
  • Metabolic alterations of cancer cells / Marco Sciacovelli, Edoardo Gaude, Mika Hilvo, and Christian Frezza -- Autophagy and cancer metabolism / Juliet Goldsmith, Beth Levine, and Jayanta Debnath -- Regulation of cancer metabolism by oncogenes and tumor suppressors / Raffaella Iurlaro, Clara Lucía León-Annicchiarico, and Cristina Muñoz-Pinedo -- Cross talk between cell death regulation and metabolism / Simone Fulda -- Techniques to monitor glycolysis / Tara TeSlaa and Michael A. Teitell -- Measurement of enolase activity in cell lysates / Keigo Fukano and Kazuhiro Kimura / Extracellular flux analysis to monitor glycolytic rates and mitochondrial oxygen consumption / Martin Pelletier, Leah K. Billingham, Madhu Ramaswamy, and Richard M. Siegel -- Conventional techniques to monitor mitochondrial oxygen consumption / Hélène Simonnet, Arnaud Vigneron, and Jacques Pouysségur -- Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry / Gerhard Krumschnabel, Andrea Eigentler, Mario Fasching, and Erich Gnaiger -- Kinetic analysis of local oxygenation and respiratory responses of mammalian cells using intracellular oxygen-sensitive probes and time-resolved fluorometry / Alexander V. Zhdanov, Ruslan I. Dmietriev, James Hynes, and Dmitri B. Papkovsky -- Cell-based measurements of mitochondrial function in human subjects / Ju-Gyeong Kang, Ping-yuan Wang, and Paul M. Hwang -- Use of chemical probes to detect mitochondrial ROS by flow cytometry and spectrofluorometry / Jing Chen and Clayton E. Mathews -- Methods to monitor ROS production by fluorescence microscopy and fluorometry / Aleksandra Wojtala, Massimo Bonora, Dominika Malinska, Paolo Pinton, Jerzy Duszynski, and Mariusz R. Wieckowski -- Genetically encoded redox sensor / Wai Kan Chiu, Atif Towheed, and Michael J. Palladino -- Use of genetically encoded sensors to monitor cytosolic ATP/ADP ratio in living cells / Andrei I. Tarasov and Guy A. Rutter -- Methods to monitor and compare mitochondrial and glycolytic ATP production / Simone Patergnani, Federica Baldassari, Elena De Marchi, Agnieszka Karkucinska-Wieckowska, Mariusz R. Wieckowski, and Paolo Pinton -- Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption / Christos Chinopoulos, Gergely Kiss, Hibiki Kawamata, and Anatoly A. Starkov -- Real-time assessment of the metabolic profile of living cells with genetically encoded NADH sensors / Yuzheng Zhao, Yi Yang, and Joseph Loscalzo -- 13C isotope-assisted methods for quantifying glutamine metabolism in cancer cells / Jie Zhang, Woo Suk Ahn, Paulo A. Gameiro, Mark A. Keibler, Zhe Zhang, and Gregory Stephanopoulos -- Measurement of fatty acid oxidation rates in animal tissues and cell lines / Frank K. Huynh, Michelle F. Green, Timothy R. Koves, and Matthew D. Hirschey -- Methods to assess lipid accumulation in cancer cells / Jørgen Sikkeland, Yang Jin, and Fahri Saatcioglu -- Analysis of hypoxia-induced metabolic preprogramming / Chendong Yang, Lei Jiang, Huafeng Zhang, Larissa A. Shimoda, Ralph J. Bernardinis, and Gregg L. Semenza.
  • To cope with the steadily growing body of knowledge and for organizational reasons the articles in this book are focussed on the modifiers ubiquitin, SUMO1/2/3, NED 8, ISG15 and FAT10 but it should be at least mentioned here that enzymes involved in conjugation and deconjugation of URM1, UFM1, ATG8 and ATG12 have been reported and continue to be investigated.
  • pt. B ScienceDirect
  • History / Franca Buffoni -- Nomenclature and potential functions of copper amine oxidases / Sinéad Boyce ... [et al.] -- Cofactors of amine oxidases : copper ion and its substitution and the 2,4,5-trihydroxylphenylalanine quinone / Shinnichiro Suzuki ... [et al.] -- Copper amine oxidases from plants / Rosaria Medda ... [et al.] -- Soluble copper amine oxidases from mammals / Paola Pietrangeli ... [et al.] -- Membrane-bound copper amine oxidases / Andrew Holt -- Copper amine oxidase genes / Ivo Frébort and Hubert G. Schwelberger -- Mechanism of TPQ biogenesis in prokaryotic copper amine oxidase / Toshihide Okajima and Katsuyuki Tanizawa -- Copper amine oxidase crystal structures / J. Mitchell Guss, Giuseppe Zanotti, and Tiina A. Salminen -- Plasma amine oxidases in various clinical conditions and in apoptosis / Frans Boomsma, Anton H. van den Meiracker, and Antonio Toninello -- Copper amine oxidases in intestinal diseases / Wieslawa Agnieszka Fogel, Ewa Toporowska-Kowalska, and Anna Stasiak -- Copper amine oxidases in adipose tissue-related disorders / Christian Carpéné -- Copper amine oxidases in adhesion molecules in renal pathology and in Alzheimer's disease and VAP-1 in leukocyte migration / Peter Boor ... [et al.] -- Inhibitors of copper amine oxidases : past, present, and future / Marek Šebela and Lawrence M. Sayre -- Pharmacological applications of copper amine oxidases / Emanuela Masini and Laura Raimondi -- Copper amine oxidases as antioxidant and cardioprotective agents / Mircea Alexandru Mateescu and Réginald Nadeau -- Biotechnological aspects of copper amine oxidases / Roberto Stevanato -- Concluding remarks / David M. Dooley.
  • Phylogenetic, structural, and functional relationships between WD and kelch repeat proteins / Andrew M. Hudson and Lynn Cooley -- Diversity of WD repeat proteins / Temple F. Smith -- A brief history of the coronin family / Eugenio L. de Hostos -- Molecular phylogeny and evolution of the coronin gene family / Reginald O. Morgan and M. Pilar Fernandez -- Coronin structure and implications / Bernadette McArdle and Andreas Hofmann -- Coronin : the double-edged sword of actin dynamics / Meghal Gandhi and Bruce L. Goode -- Invertebrate coronins / Maria C. Shina and Angelika A. Noegel -- Evolutionary and functional diversity of coronin proteins / Charles Peter Xavier ... [et al.] -- Role of mammalian coronin 7 in the biosynthetic pathway / Vasily Rybaki -- Coronin 1 in innate immunity / Jean Pieters -- The role of mammalian coronins in development and disease / David W. Roadcap, Christoph S. Clemen, and James E. Bear.
  • Current Protocols in Chemical Biology provides validated protocols and overviews about specialized chemical techniques and tools for studies of biology and drug design. These tools include small-molecule design, synthesis, derivatization, handling, and detection as well as advances in laboratory automation, robotics, and medicinal chemistry as applied to high-throughput screening (HTS). Methods for modification of proteins, nucleic acids, carbohydrates and lipids for their use as tools in the study of particular biological systems are also included
  • Cyclooxygenases methods and protocols 2008, Springer Protocols
    Part I. In vitro protocols for studying expression and activity of cyclooxygenase enzymes and characterisation of their inhibition patterns -- 1. In vitro cyclo-oxygenase expression and activity protocols : introduction to Part I / Roderick J. Flower -- 2. Techniques used to study regulation of cyclooxygenase-2 promoter sites / Hiroyasu Inoue and Rieko Nakata -- 3. Purification of recombinant human COX-1 and COX-2 / James K. Gierse -- 4. Cloning and expression of cyclooxygenase-1 and cyclooxygenase-2 / Nicholas R. Staten and Beverly A. Reitz -- 5. Expression of cyclooxygenase isoforms in the baculovirus expression system / John C. Hunter, Natalie M. Myres, and Daniel L. Simmons -- 6. Peroxidase active site activity assay / Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett -- 7. Study of inhibitors of the PGH synthases for which potency Is regulated by the redox state of the enzymes / Olivier Boutaud and John A. Oates -- 8. Different methods for testing potential cyclooxygenase-1 and cyclooxygenase-2 inhibitors / Stefan Laufer and Sabine Luik -- 9. In vitro cyclooxygenase activity assay in tissue homogenates / Samir S. Ayoub -- 10. Techniques used to characterize the binding of cyclooxygenase Inhibitors to the cyclooxygenase active site / William F. Hood -- 11. In vitro Assays for cyclooxygenase activity and inhibitor characterization / Mark C. Walker and James K. Gierse -- -- Part II. Extraction and measurement of prostanoids and isoprostanes -- 12. Extraction and measurement of prostanoids and isoprostanes : introduction to Part II / Paola Patrignani -- 13. Prostanoid extraction and measurement / Lorenzo Polenzani and Samir S. Ayoub -- 14. Measurement of 8-iso-prostaglandin F2a in biological fluids as a measure of lipid peroxidation / Stefania Tacconelli, Marta L. Capone, and Paola Patrignani -- -- Part III. In vivo models to study involvement of cyclooxygenase products in health and disease -- 15. In vivo models to study cyclooxygenase products in health and disease: introduction to Part III / Derek W. Gilroy, Melanie Stables, and Justine Newson -- 16. Protocols to assess the gastrointestinal side effects resulting from inhibition of Cyclo-Oxygenase isoforms / Brendan J.R. Whittle -- 17. Cyclooxygenase enzymes and their products in the carrageenan-induced pleurisy in rats / Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed -- 18. Iloprost-induced nociception : determination of the site of anti-nociceptive action of cyclooxygenase inhibitors and the Involvement of cyclooxygenase products in central mechanisms of nociception / Samir S. Ayoub and Regina M. Botting.
  • The book deals with various clinical aspects of cytochrome P450 2E1 (CYP2E1) which is a potent source for oxidative stress. Oxidative stress is critical for pathogenesis of diseases and CYP2E1 is a major contributor for oxidative stress. Several clinical disorders are associated with changes in regulation of CYP2E1 and the consequent abnormalities which include alcoholic liver disease, alcoholic pancreatitis, carcinogenesis, non-alcoholic fatty liver disease, non-alcoholic steatohepatitis, obesity, hepatitis C virus infection, reproductive organ toxicity, hepatocellular and cholestatic liver cirrhosis, inhibition of bone repair, cross-tolerance in smokers and people treated with nicotine, disorders of central nervous system, changes in metabolism of protoxicants in the circulatory system and susceptibility to human papillomavirus infection. Hence, CYP2E1 emerges as a new and potent player in aggravating injury and furthering disease complications.
  • Cytochrome P450 protocols. 2nd ed. 2005, Springer Protocols
    Cytochrome P450 nomenclature, 2004 / David R. Nelson -- Spectral analyses of cytochromes P450 / John B. Schenkman and Ingela Jansson -- Establishment of functional human cytochrome P450 monooxygenase systems in Escherichia coli / Michael P. Pritchard, Lesley McLaughlin, and Thomas Friedberg -- Purification of cytochromes P450: products of bacterial recombinant expression systems / F. Peter Guengerich and Martha V. Martin -- Expression of recombinant flavin-containing monooxygenases in a baculovirus/insect cell system / Azara Janmohamed ... [et al.] -- Cytochrome P450 reconstitution systems / Hiroshi Yamazaki and Tsutomu Shimada -- Catalytic assays for human cytochrome P450: an introduction / Thomas K.H. Chang and David J. Waxman -- Enzymatic analysis of cDNA-expressed human CYP1A1, CYP1A2, and CYP1B1 with 7-ethoxyresorufin as substrate / Thomas K.H. Chang and David J. Waxman -- Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydroxylation / David J. Waxman and Thomas K.H. Chang -- Determination of CYP2B6 component of 7-ethoxy-4-trifluoromethylcoumarin O-deethylation activity in human liver microsomes / Thomas K.H. Chang, Charles L. Crespi, and David J. Waxman -- High-performance liquid chromatography analysis of CYP2C8-catalyzed paclitaxel 6[alpha]-hydroxylation / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman -- Determination of CYP2C0-catalyzed diclofenac 4'-hydroxylation by high-performance liquid chromatography / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman -- CYP2C19-mediated (S)-mephenytoin 4'-hydroxylation assayed by high-performance liquid chromatography with radiometric detection / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman -- CYP2D6-dependent bufuralol 1'-hydroxylation assayed by reverse-phase ion-pair high-performance liquid chromatography with fluorescence detection / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman -- Spectrophotometric analysis of human CYP2E1-catalyzed p-nitrophenol hydroxylation / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman -- Thin-layer chromotography analysis of human CYP23A-catalyzed testosterone 6[beta]-hydroxylation / David J. Waxman and Thomas K.H. Chang -- Determination of CYP4A11-catalyzed lauric acid 12-hydroxylation by high-performance liquid chromotography with radiometric detection / Charles L. Crespi, Thomas K.H. Chang, and David J. Waxman -- An isocratic high-performance liquid chromatography assay for CYP7A1-catalyzed cholesterol 7[alpha]-hydroxylation / David Waxman and Thomas K.H. Chang -- Use of 7-ethoxycoumarin to monitor multiple enzymes in the human CYP1, CYP2, and CYP3 families / David J. Waxman and Thomas K.H. Chang -- Benzydamine N-oxygenation as a measure of flavin-containing monooxygenase activity / Catherine K. Yeung and Allan E. Rettie -- Prochiral sulfoxidation as a probe for flavin-containing monooxygenases / Catherine K. Yeung and Allan E. Rettie -- Targeting antipeptide antibodies toward cytochrome P450 enzymes / Robert J. Edwards -- The Human Cytochrome P450 Allele Nomenclature Committee web site: submission criteria, procedures, and objectives / Sarah C. Sim and Magnus Ingelman-Sundberg -- Genotyping for cytochrome P450 polymorphisms / Ann K. Daly, Barry P. King, and Julian B.S. Leathart -- Hepatocyte cultures in drug metabolism and toxicological research and testing / Tamara VanHaecke and Vera Rogiers -- Isolation of rat hepatocytes / Peggy Papeleu ... [et al.] -- Rat hepatocyte cultures: conventional monolayer and cocultures with rat liver epithelial cells / Tom Henkens ... [et al.] -- Rat hepatocyte cultures: collagen gel sandwich and immobilization cultures / Mathieu Vinken ... [et al.] -- Hepatocytes in suspension / Greetje Elaut ... [et al.] -- Isolation of rat bone marrow stem cells / Sarah Snykers, Tamara Vanhaecke, and Vera Rogiers -- Transfection of primary cultures of rat hepatocytes / Mina Edwards -- Human hepatocyte culture / Lydiane Pichard ... [et al.] -- Determination of cellular localization of the expression of flavin-containing monooxygenase genes in mouse tissues by in situ hybridization / Azara Janmohamed, Ian R. Phillips, and Elizabeth A. Shephard -- Deletion of genes from the mouse genome using cre/loxP technology / Diana Hernandez ... [et al.] -- Characterization of targeted mouse embryonic stem cell chromosomes: karyotyping and fluorescence in situ hybridization of metaphase spreads / Diana Hernandez, Ian R. Phillips, and Elizabeth A. Shephard -- Microinjection of targeted embryonic stem cells and establishment of knockout mouse lines for fmo genes / Diana Hernandez ... [et al.] -- Use of reporter genes to measure xenobiotic-mediated activation of CYP gene transcription / Nick Plant.
  • Cytochrome P450. Part C 2002, ScienceDirect
  • Instruments and methods in proteomics -- In-depth protein characterization by mass spectrometry -- Analysis of phosphoproteomics data -- The origin and early reception of sequence databases -- Laboratory data and sample management for proteomics -- PRIDE and "Database on demand" As valuable tools for computational proteomics -- Analysing proteomics identifications in the context of functional and structural protein annotation: Integrating annotation using PICR, DAS, and BioMart -- Tranche distributed repository and proteomecommons.Org -- Data standardization by the HUPO-PSI: How has the community benefitted? -- mzIdentML: An open community-built standard format for the results of proteomics spectrum identification algorithms -- Spectra, chromatograms, metadata: mzML-the standard data format for mass spectrometer output -- imzML: Imaging mass spectrometry markup language: A common data format for mass spectrometry imaging -- Tandem mass spectrometry spectral libraries and library searching -- Inter-lab proteomics: Data mining in collaborative projects on the basis of the HUPO brain proteome project's pilot studies -- Data management and data integration in the HUPO plasma proteome project -- Statistics in experimental design, preprocessing, and analysis of proteomics data -- The evolution of protein interaction networks -- Cytoscape: Software for visualization and analysis of biological networks -- Text mining for systems modeling -- Identification of alternatively spliced transcripts using a proteomic informatics approach -- Distributions of ion series in ETD and CID spectra: Making a comparison -- Evaluation of peak-picking algorithms for protein mass spectrometry -- OpenMS and TOPP: Open source software for LC-MS data analysis -- LC/MS data processing for label-free quantitative analysis -- Spectral properties of correlation matrices - towards enhanced spectral clustering -- Standards, databases, and modeling tools in systems biology -- Modeling of cellular processes: Methods, data, and requirements.
  • "A comprehensive assessment of biomarkers, this book covers the history and current status of the application of biomarkers in diagnostics and prognostics. It explores the technology used for the study of biomarkers, and the validation of biomarkers including a comparison of the various technologies used to identify and measure biomarkers. The editors emphasize the technology underlying biomarkers and the translation of basic science to clinical laboratory technology, including the commercial development of biomarkers. The book also covers proteomics and proteomic technologies and their applications in the identification of biomarkers"--Provided by publisher.
  • Diagnostic Immunohistochemistry presents the latest information and most reliable guidance on immunohistological diagnoses in surgical pathology. David J. Dabbs, MD and other leading experts bring you state-of-the-art coverage on genomic and theranostic applications, molecular anatomic pathology, immunocytology, Non-Hodgkin's lymphoma, and more. Additional features such as tables discussing antibody specifications, differential diagnosis boxes, ancillary anatomic molecular diagnostics, and full-color histological images ensure user-friendly coverage that makes key information easy to find and apply. The fully searchable text is also available online at, along with a downloadable image bank and access to Path Consult. This concise and complete resource is today's indispensable guide to the effective use of immunohistochemical diagnosis. Discusses diagnostic pitfalls through immunohistologic differential diagnosis wherever appropriate so you can provide the most accurate diagnoses. Presents chapters arranged by organ system for comprehensive coverage of all relevant information in a convenient and intuitive organization. Provides quick reference graphs for antibodies throughout the text that illustrate the frequency of immunostaining for a variety of antibodies in tumors. Includes Key Diagnostic Points boxes in every chapter for a quick summary of text areas that are of particular importance. Features an expert author for each chapter to ensure coverage of the current state of the art.
  • Topic I. Structure and function. Peptide substrates of dipeptidyl peptidases / Inger Brandt ... [et al.] Phosphorus-containing inhibitors of proteolytic enzymes / Angel Stöckel-Maschek ... [et al.] Biochemical properties of recombinant prolyl dipeptidases DPP-IV and DPP8 / Xin Chen. Prediction of dipeptidyl peptidase (DP) 8 structure by homology modelling / Melissa R. Pitman, R. Ian Menz, and Catherine A. Abbott -- Topic II. DPIV-related enzymes. Structure and function in dipeptidyl peptidase IV and related proteins / Mark D. Gorrell ... [et al.] Expression and function of dipeptidyl peptidase IV and related enzymes in cancer / Petr Busek ... [et al.] DP8 and DP9 have extra-enzymatic roles in cell adhesion, migration, and apoptosis / Denise M.T. Yu ... [et al.] In vivo effects of a potent, selective DPPII inhibitor: UAMC00039 is a possible tool for the elucidation of the physiological function of DPPII / Marie-Berthe Maes ... [et al.] Expression of dipeptidyl peptidase IV-like enzymes in human peripheral blood mononuclear cells / Sabine Wrenger ... [et al.] Dipeptidyl peptidase 8 has post-proline dipeptidyl aminopeptidase and prolyl endopeptidase activities / Joohong Park ... [et al.] Prolyl endopeptidase cleaves the apoptosis rescue peptide humanin and exhibits and unknown post-cysteine cleavage specificity / Joachim Wolfgang Bär ... [et al.] Distribution of dipeptidyl peptidase IV-like activity enzymes in canine and porcine tissue sections by RT-PCR / Leona Wagner ... [et al.] -- Topic III. Metabolic disorders. Relative contribution of incretins to the glucose lowering effect of DP IV inhibitors in type 2 diabetes mellitus (T2DM) / Simon A. Hinke, Raymond A. Pederson, and Christopher H.S. McIntosh. Dipeptidyl peptidase IV: a molecular switch of vascular actions of neuropeptide Y / Lijun Li, Hans-Ulrich Demuth, and Zofia Zukowska -- Topic IV. Immune disorders. Dipeptidylpeptidase IV (DPIV) and alanyl-aminopeptidases (AAPs) as a new target complex for treatment of autoimmune and inflammatory diseases: proof of concept in a mouse model of colitis / Ute Bank ... [et al.] Dipeptidyl peptidases and inflammatory bowel disease / Catherine A. Abbott ... [et al.] Possible role of DP IV inhibitors in acne therapy / Anja Thielitz ... [et al.] Dipeptidyl peptidase-IV activity and/or structure homologs (DASH): contributing factors in the pathogenesis of rheumatic diseases? / Eva Balaziova ... [et al.] -- Topic V. Neuronal diseases. Dipeptidyl peptidase IV (DP IV, CD26) and aminopeptidase N (APN, CD13) as regulators of T cell function and targets of immunotherapy in CNS inflammation / Aliza Biton ... [et al.] CD26/DP IV in T cell activation and autoimmunity / Vera Preller ... [et al.] DPIV/CD26 and FAP in cancer: a tale of contradictions / Melanie L. Sulda, Catherine A. Abbott, and Martin Hildebrandt. Type-II transmembrane prolyl dipeptidases and matrix metalloproteinases in membrane vesicles of active endothelial cells / Monica Salamone ... [et al.] Extra-enzymatic roles of DPIV and FAP in cell adhesion and migration on collagen and fibronectin / Xin M. Wang ... [et al.] Role of neuropeptide Y and dipeptidyl peptidase IV in regulation of Ewing's sarcoma growth / Joanna Kitlinska ... [et al.] The role of CD26/DPP IV in preservation of early pulmonary graft function / Florian Johannes Jung ... [et al.].
  • ch. 1. Introduction, p. 1-3 / Kai Johnsson and Susanne Brakmann, p. 1-3 -- ch. 2. Evolutionary Biotechnology - From Ideas and Concepts to Experiments and Computer Simulations / Peter Schuster, p. 5-28 -- ch. 3. Using Evolutionary Strategies to Investigate the Structure and Function of Chorismate Mutases / Donald Hilvert, Sean V. Taylor and Peter Kast, p. 29-62 -- ch. 4. Construction of Environmental Libraries for Functional Screening of Enzyme Activity / Rolf Daniel, p. 63-78 -- ch. 5. Investigation of Phage Display for the Directed Evolution of Enzymes / Patrice Soumillion and Jacques Fastrez, p. 79-110 -- ch. 6. Directed Evolution of Binding Proteins by Cell Surface Display: Analysis of the Screening Process / K. Dane Wittrup, p. 111-126 -- ch. 7. Yeast n-Hybrid Systems for Molecular Evolution / Brian T. Carter, Hening Lin and Virginia W. Cornish, p. 127-158 -- ch. 8. Advanced Screening Strategies for Biocatalyst Discovery / Andreas Schwienhorst, p. 159-175 -- ch. 9. Engineering Protein Evolution / Stefan Lutz and Stephen J. Benkovic, p. 177-213 -- ch. 10. Exploring the Diversity of Heme Enzymes through Directed Evolution / Patrick C. Cirino and Frances H. Arnold, p. 215-243 -- ch. 11. Directed Evolution as a Means to Create Enantioselective Enzymes for Use in Organic Chemistry / Manfred T. Reetz and Karl-Erich Jaeger, p. 245-279 -- ch. 12. Applied Molecular Evolution of Enzymes Involved in Synthesis and Repair of DNA / John F. Davidson, Jon Anderson, Haiwei Guo, Daniel Landis and Lawrence A. Loeb, p. 281-307 -- ch. 13. Evolutionary Generation Versus Rational Design of Restriction Endonucleases with Novel Specificity / Thomas Lanio, Albert Jeltsch and Alfred Pingoud, p. 309-327 -- ch. 14. Evolutionary Generation of Enzymes with Novel Substrate Specificities / Uwe T. Bornscheuer, p. 329-341.
  • Applied enzymology: an overview / Nancy Shanley and Gary Walsh -- Enzyme engineering / Barry M. McGrath -- Tissue plasminogen activator-based thrombolytic agents / Gary Walsh -- Activated protein C / Brian W. Grinnell, S. Betty Yan, and William L. Macias -- Deoxyribonuclease I / Niek N. Sanders, Stefaan C. de Smedt, and Joseph Demeester -- SS-glucocerebrosidase ceredase and cerezyme / Tim Edmunds -- A-galactosidase / Debra Barngrover -- Urate oxidase / Alain Bayol ... [et al.] -- L-asparaginase review of pharmacology, drug resistance, and clinical applications / Christine Mauz-Korholz, Volker Wahn, and Dieter Korholz -- Recombinant factor VIIa / Elisabeth Erhardtsen ... [et al.] -- Factor IX (protease zymogen) / Barry M. McGrath -- The development of aldurazyme (laronidase) / Emil Kakkis -- Additional therapeutic enzymes / Shane O'Connell.
  • DNA methylation : an introduction to the biology and the disease-associated changes of a promising biomarker / Jörg Tost -- Quantification of global DNA methylation by capillary electophoresis and mass spectrometry / Maria Berdasco, Mrio F. Fraga, and Manel Esteller -- Methyl group acceptance assay for the determination of global DNA methylation levels / Kenneth P. Nephew, Curt Balch, and David G. Skalnik -- Immunodetection array / Johannes Pröll ... [et al.] -- Methylated DNA immunoprecipitation (MeDIP) / Fabio Mohn ... [et al.] -- The MIRA method for DNA methylation analysis / Tibor A. Rauch and Gerd P. Pfeifer -- The HELP assay / Mayumi Oda and John M. Greally -- Differential methylation hybridization : profiling DNA methylation with a high-density CpG island microarray / Pearlly S. Yan ... [et al.] -- Analysis of DNA methylation by amplification of intermethylated sites (AIMS) / Mireia Jordà ... [et al.] -- Methylation-sensitive representational difference analysis (MS-RDA) / Toshikazu Ushijima and Satoshi Yamashita -- Restriction landmark genomic scanning : analysis of CpG islands in genomes by 2D gel elctrophoresis / Joseph F. Costello ... [et al.] -- GoldenGate assay for DNA methylation profiling / Marina Bibikova and Jian-Bing Fan -- 5'-azacytidine expression arrays / Paul Cairns -- DNA methylation analysis by bisulfite conversion, cloning, and sequencing of individual clones / Yingying Zhang ... [et al.] -- Identification and quantification of differentially methylated loci by the Pyrosequencing technology / Emelyne Dejeux ... [et al.] -- Mass spectrometric analysis of cytosine methylation by base-specific cleavage and primer extension methods / Dirk van den Boom and Mathias Ehrich -- Melting curve assays for DNA methylation analysis / Tomasz K. Wojdacz and Alexander Dobrovic -- Methylatioin SNaPshot : a method for the quantification of site-specific DNA methylation levels / Zachary Kaminsky and Arturas Petronis -- Bio-COBRA : absolute quantification of DNA methylation in electrofluidics chips / Romulo Martin Brena and Christoph Plass -- Restriction digestion and real-time PCR (qAMP) / Christopher C. Oakes ... [et al.] -- MethylQuant : a real-time PCR-based method to quantify DNA methylation at single specific cytosines / Claire Dugast-Darzacq and Thierry Grange -- Methylation-specific PCR / Julien D.F. Licchesi and James G. Herman -- MethyLight / Mihaela Campan ... [et al.] -- Quantification of methylated DNA by HeavyMethyl duplex PCR / Jürgen Distler -- Analysis of methylated circulating DNA in cancer patients' blood / Eiji Sunami ... [et al.] -- Prevention of PCR cross-contamination by UNG treatment of bisulfite-treated DNA / Reimo Tetzner -- Profiling DNA methylation from small amounts of genomic DNA starting material : efficient sodium bisulfite conversion and subsequent whole-genome amplification / Jonathan Mill and Arturas Petronis.
  • "ENOX Proteins" documents a unique family of cell surface proteins which are the exclusive discovery (subsequently confirmed by others) of the authors, their students and their colleagues. An ENOX-based mechanism is provided for how cells increase in size that is both unique and applicable not only to cancer and cancer therapy but also to production agriculture and biomass increase. The role of ENOX proteins in biological time-keeping is described along with evidence for oscillations in the ratios of electron spin pairs defining ortho and para water states that form highly coordinated populations of coherent water of interest to physicists as the underlying mechanism. The age-related NADH oxidases that appear around age 30 and increase steadily thereafter with potentially causal involvements in atherogenesis and skin aging have been identified as five members of the TM-9 protein superfamily of all with different chromosomal locations. The arNOX proteins initially are membrane anchored but functionally-active N-terminal fragments that are shed into body fluids. Except for critical functional motifs, all of the ENOX protein family members have distinct protein sequences and chromosomal origins. A fourth member of the ENOX protein family occurs in plants as the primary target for both natural and synthetic growth hormone (auxin)-stimulated rapid cell enlargement. Despite masquerading as intractable and difficult to clone and characterize, ENOX proteins offer remarkable opportunities for research, commercial development and outside confirmation of new paradigms to help explain complex biological processes.
  • Bonus Student Consult access - included with the text - allows readers to conveniently access the book's content online. This concise and user-friendly reference provides crucial knowledge for the early years of medical training, as well as for exam preparation.
  • In biochemistry, a metalloprotein is a generic term for a protein that contains a metal cofactor. The metal may be an isolated ion or may be coordinated with a nonprotein organic compound, such as the porphyrin found in hemoproteins. In some cases, the metal is co-coordinated with a side chain of the protein and an inorganic nonmetallic ion. This kind of protein-metal-nonmetal structure is seen in iron-sulfur clusters Metalloproteins deals with all aspects related to the intracellular and extracellular metal-binding proteins, including their structures, properties and functions. The biological roles of metal cations and metal-binding proteins are endless. They are involved in all crucial cellular activities. Many pathological conditions are related to the problematic metal metabolism. Research in metalloprotein-related topics is therefore rapidly growing, and different aspects of metal-binding proteins progressively enter curricula at Universities and even at the High School level on occasion. However, no key resource providing basic, but comprehensible knowledge on this rapidly expanding field exists. The Encyclopedia of Metalloproteins aims to bridge this gap, and will attempt to cover various aspects of metalloprotein/metalloproteomics and will deal with the different issues related to the intracellular and extracellular metal-binding proteins, including their structures, properties and functions. The goal is to cover exhaustively all catalytically and biologically crucial metal ions and to find at least one interacting protein for other metal ions. The Encyclopedia of Metalloproteins will provide a key resource for advanced undergraduate and graduate students, researchers, instructors, and professors interested in protein science, biochemistry, cell biology, and genetics.
  • Endotoxins: Lipopolysaccharides of Gram-negative bacteria / X. Wang and P.J. Quinn -- Purification and characterization of lipopolysaccharides / X. Wang ... [et al.] -- Endotoxins: Relationship between structure, function, and activity / K. Brandenburg, A.B. Schromm and T. Gutsmann -- The diversity of the core oligosaccharide in lipopolysaccharides / A. Silipo and A. Molinaro -- Salmonella-regulated lipopolysaccharide modifications / S.M. Richards, K.L. Strandberg and J.S. Gunn -- The variation of O antigens in Gram-negative bacteria / L. Wang, Q. Wang and P.R. Reeves -- Regulators of TLR4 signaling by endotoxins / A.F. McGettrick and L.A.J. O'Neill -- Membrane partitioning: Is location everything when it comes to endotoxin recognition? / M. Triantafilou and K. Triantafilou -- Endotoxin detection - from limulus amebocyte lysate to recombinant Factor C / J.L. Ding and B. Ho -- The role of endotoxin in infection: Helicobacter pylori and Campylobacter jejuni / A.P. Moran -- The role of pseudomonas lipopolysaccharide in cystic fibrosis airway infection / S.M. Moskowitz and R.K. Ernst -- Development of small-molecule endotoxin sequestering agents / S.A. David and D. Sil -- Development of an anti-endotoxin vaccine for sepsis / A.S. Cross -- Synthetic and natural TLR4 agonists as safe and effective vaccine adjuvants / C.B. Fox ... [et al.] -- Targeting endotoxin in the treatment of sepsis / J.-S. Rachoin, C.A. Schorr and R.P. Dellinger -- Lipopolysaccharides in rhizobium-legume symbioses / R.W. Carlson, L.S. Forsberg and E.L. Kannenberg -- Lipopolysaccharides and plant innate immunity / G. Erbs ... [et al.].
  • The generation of zinc finger proteins by modular assembly / Mital S. Bhakta and David J. Segal -- Engineering single Cys2His2 zinc finger domains using a bacterial cell-based two-hybrid selection system / Stacey Thibodeau-Beganny, Morgan L. Maeder, and J. Keith Joung -- Bipartite selection of zinc fingers by phage display for any 9-bp DNA target site / Jia-Ching Shieh -- Structure-based DNA-binding prediction and design / Andreu Alibés, Luis Serrano, and Alejandro D. Nadra -- Generation of cell-permeable artificial zinc finger protein variants / Takashi Sera -- Inhibition of viral transcription using designed zinc finger proteins / Kimberley A. Hoeksema and D. Lorne J. Tyrrell -- Modulation of gene expression using zinc finger-based artificial transcription factors / Sabine Stolzenburg ... [et al.] -- Construction of combinatorial libraries that encode zinc finger-based transcription factors / Seokjoong Kim, Eun Ji Kim, and Jin-Soo Kim -- Silencing of gene expression by targeted DNA methylation : concepts and approaches / Renata Z. Jurkowska and Albert Jeltsch -- Remodeling genomes with artificial transcription factors (ATFs) / Adriana S. Beltran and Pilar Blancafort -- Transgenic mice expressing an artificial zinc finger regulator targeting an endogenous gene / Claudio Passananti ... [et al.] -- Artificial zinc finger nucleases for DNA cloning / Vardit Zeevi, Andriy Tovkach, and Tzvi Tzfira -- In vitro assessment of zinc finger nuclease activity / Toni Cathomen and Cem Șöllü -- Quantification of zinc finger nuclease-associated toxicity / Tatjana I. Cornu and Toni Cathomen -- A rapid and general assay for monitoring endogenous gene modification / Dmitry Y. Guschin ... [et al.] -- Engineered zinc finger proteins for manipulation of the human mitochondrial genome / Michal Minczuk -- High-efficiency gene targeting in Drosophila with zinc finger nucleases / Dana Carroll, Kelly J. Beumer, and Jonathan K. Trautman -- Using zinc finger nucleases for efficient and heritable gene disruption in zebrafish / Jasmine M. McCammon and Sharon L. Amacher -- A transient assay for monitoring zinc finger nuclease activity at endogenous plant gene targets / Justin P. Hoshaw ... [et al.] -- Validation and expression of zinc finger nucleases in plant cells / Andriy Tovkach, Vardit Zeevi, and Tzvi Tzfira -- Non-FokI-based zinc finger nucleases / Miki Imanishi, Shigeru Negi, and Yukio Sugiura -- Designing and testing chimeric zinc finger transposases / Matthew H. Wilson and Alfred L. George -- Seeing genetic and epigenetic information without DNA denaturation using sequence-enabled reassembly (SEER) / Jason R. Porter ... [et al.] -- Zinc finger-mediated live cell imaging in Arabidopsis roots / Beatrice I. Lindhout, Tobias Meckel, and Bert J. van der Zaal -- Biophysical analysis of the interaction of toxic metal ions and oxidants with the zinc finger domain of XPA / Andrea Hartwig, Tanja Schwerdtle, and Wojciech Bal -- Preparation and zinc-binding properties of multi-fingered zinc-sensing domains / John H. Laity and Linda S. Feng -- Using ChIP-seq technology to identify targets of zinc finger transcription factors / Henriette O'Geen, Seth Frietze, and Peggy J. Farnham -- Crystallization of zinc finger proteins bound to DNA / Nancy C. Horton and Chad K. Park -- Beyond DNA : zinc finger domains as RNA-binding modules / Josep Font and Joel P. Mackay.
  • Enzymatic transformation 2013, Springer
    Transformations using enzymes have been extensively investigated in the last two decades and the results promise great potential for this growing field, especially in the area of synthetic organic chemistry mainly due to of its many advantages. Accordingly, this book has attempted to bring out the advantages of using enzymes involving complex underivatized and unprotected substrates in non-polar media under homogenous and heterogeneous reaction conditions. Merits and demerits of using enzymes in terms of yields and selectivity/specificity are presented without any prejudice. Almost all the reactions dealt with are from the author's laboratory comprising diverse substrates, and the catalysis involves two important hydrolyzing enzymes, extensively examined for the reverse reactions. Thus, esterification involving lipses and glycosylation involving glycosidases were investigated with respect to various strategies like optimization of reaction conditions, response surface methodology and kinetics, carrying out reactions under solvent, non-solvent and super critical carbon dioxide conditions. In short, the work presented is to ensure the comprehension of the problems faced by the researchers in this area so as to work out further efficient strategies for carrying out enzymatic transformations in the laboratory successfully with better yields and specificity.
  • Chapter 1. An Introduction to Enzyme Science -- Chapter 2. Active Sites and their Chemical Properties -- Chapter 3. Fundamentals of Chemical Kinetics -- Chapter 4. Practical Aspects of Measuring Initial Rates and Reaction Parameters -- Chapter 5. Initial-Rate Kinetics of One-Substrate Enzyme-Catalyzed Reactions -- Chapter 6. Initial-Rate Kinetics of Multi-Substrate Enzyme-Catalyzed Reactions -- Chapter 7. Factors Influencing Enzyme Activity -- Chapter 8. Kinetic Behavior of Enzyme Inhibitors -- Chapter 9. Isotopic Probes of Biological Catalysis -- Chapter 10. Probing Fast Enzyme Processes -- Chapter 11. Regulatory Behavior of Enzymes -- Chapter 12. Single-Molecule Enzyme Kinetics -- Chapter 13. Mechanoenzymes: Catalysis, Force Generation and Kinetics.
  • Introduction, p. 1-3 -- ch. 1. Multiple Equilibria, p. 5-50 -- ch. 2. Enzyme Kinetics, p. 51-160 -- ch. 3. Methods, p. 161-246.
  • Introduction to the field of enzyme immobilization and stabilization -- Stabilization of enzymes through encapsulation in liposomes -- Micellar enzymology for thermal, pH, and solvent stability -- Lipase activation and stabilization in room-temperature ionic liquids -- Nanoporous silica glass for the immobilization of interactive enzyme systems -- Enzyme stabilization and immobilization by sol-gel entrapment -- Nanoporous gold for enzyme immobilization -- Enzyme stabilization via bio-templated silicification reactions -- The immobilization of enzyme on Eupergit) supports by covalent attachment -- Micellar polymer encapsulation of enzymes -- Enzyme stabilization via cross-linked enzyme aggregates -- Enzyme immobilization in polyelectrolyte microcapsules -- Macroporous poly(GMA-co-EGDMA) for enzyme stabilization -- Enzyme-nanoparticle conjugates for biomedical applications -- Enzyme nanoparticle fabrication: Magnetic nanoparticle synthesis and enzyme immobilization -- Cytochrome C stabilization and immobilization in aerogels -- Kinetic measurements for enzyme immobilization.
  • 1. Enzyme Activities and Microbiological and Biochemical Processes in Soil / Paolo Nannipieri, Ellen Kandeler, and Pacifico Ruggiero -- 2. Ecology of Microbial Enzymes in Lake Ecosystems / Ryszard Jan Chróst and Waldemar Siuda -- 3. Ecological Significance of Bacterial Enzymes in the Marine Environment / Hans-Georg Hoppe, Carol Arosti, and Gerhard F. Herndl -- 4. Enzymes and Microorganisms in the Rhizosphere / David C. Naseby and James M. Lynch -- 5. Enzymes in the Arbuscular Mycorrhizal Symbiosis / José Manuel García-Garrido, Juan Antonio Ocampo, and Inmaculada García-Romera -- 6. Microbes and Enzymes Associated with Plant Surfaces / Alan P. Thompson and Mark J. Bailey -- 7. Microbial Enzymes in the Biocontrol of Plant Pathogens and Pests / Leonid Cherin and Ilan Chet -- 8. Microbiology and Enzymology of Carbon and Nitrogen Cycling / Robert L. Tate III -- 9. Enzyme and Microbial Dynamics of Litter Decomposition / Robert L. Sinsabaugh, Margaret M. Carreiro, and Sergio Alvarez -- 10. Fungal Communities, Succession, Enzymes, and Decomposition / Annelise H. Kjøller and Sten Struwe -- 11. Enzyme Adsorption on Soil Mineral Surfaces and Consequences for the Catalytic Activity / Hervé Quiquampoix, Sylvie Servagent-Noinville, and Marie-Hélène Baron -- 12. Microbes and Enzymes in Biofilms / Jana Jass, Sara K. Roberts, and Hilary M. Lappin-Scott -- 13. Search for and Discovery of Microbial Enzymes from Thermally Extreme Environments in the Ocean / Jody W. Deming and John A. Baross -- 14. Molecular Methods for Assessing and Manipulating the Diversity of Microbial Populations and Processes / Søren J. Sørensen, Julia R. de Lipthay, Anne Kirstine Müller, Tamar Barkay, Lars H. Hansen, and Lasse Dam Rasmussen -- 15. Bioindicators and Sensors of Soil Health and the Application of Geostatistics / Ken Killham and William J. Staddon -- 16. Hydrolytic Enzyme Activities to Assess Soil Degradation and Recovery / Tom W. Speir and Des J. Ross -- 17. Enzymatic Responses to Pollution in Sediments and Aquatic Systems / Sabine Kuhbier, Hans-Joachim Lorch, and Johannes C.G. Ottow -- 18. Microbial Dehalogenation Reactions in Microorganisms / Lee A. Beaudette, William J. Staddon, Michael B. Cassidy, Marc Habash, Hung Lee, and Jack T. Trevors -- 19. Isolated Enzymes for the Transformation and Det6xification of Organic Pollutants / Liliana Gianfreda and Jean-Marc Bollag -- 20. Enzyme-Mediated Transformations of Heavy Metals/Metalloids: Applications in Bioremediation / Robert S. Dungan and William T. Frankenberger, Jr. -- 21. Enzymes in Soil: Research and Developments in Measuring Activities / M. Ali Tabatabai and Warren A. Dick.
  • Composition and Dynamics of the Eukaryotic Replisome: A Brief Overview / Stuart MacNeill -- Evolutionary Diversification of Eukaryotic DNA Replication Machinery / Stephen J. Aves, Yuan Liu and Thomas A. Richards -- The Origin Recognition Complex: A Biochemical and Structural View / Huilin Li and Bruce Stillman -- Archaeal Orc1/Cdc6 Proteins / Stephen D. Bell -- Cdt1 and Geminin in DNA Replication Initiation / Christophe Caillat and Anastassis Perrakis -- MCM Structure and Mechanics: What We Have Learned from Archaeal MCM / Ian M. Slaymaker and Xiaojiang S. Chen -- The Eukaryotic Mcm2-7 Replicative Helicase / Sriram Vijayraghavan and Anthony Schwacha -- The GINS Complex: Structure and Function / Katsuhiko Kamada -- The Pol [alpha]-Primase Complex / Luca Pellegrini -- The Structure and Function of Replication Protein A in DNA Replication / Aishwarya Prakash and Gloria E. O. Borgstahl -- Structural Biology of Replication Initiation Factor Mcm10 / Wenyue Du, Melissa E. Stauffer and Brandt F. Eichman -- Structure and Function of Eukaryotic DNA Polymerase [delta] / Tahir H. Tahirov -- DNA Polymerase [epsilon] / Matthew Hogg and Erik Johansson -- The RFC Clamp Loader: Structure and Function / Nina Y. Yao and Mike O'Donnell -- PCNA Structure and Function: Insights from Structures of PCNA Complexes and Post-translationally Modified PCNA / Lynne M. Dieckman, Bret D. Freudenthal and M. Todd Washington -- The Wonders of Flap Endonucleases: Structure, Function, Mechanism and Regulation / L. David Finger, John M. Atack, Susan Tsutakawa, Scott Classen and John Tainer, et al. -- DNA Ligase I, the Replicative DNA Ligase / Timothy R. L. Howes and Alan E. Tomkinson.
  • This book provides an essential overview of the science of polysaccharides. It both approaches polysaccharides as a polymer class and provides detailed descriptions of most major polysaccharides (cellulose, mannan, xylan, chitin-chitosan, cyclodextrines). Owing to the multidisciplinary character of the European Polysaccharide Network of Excellence (EPNOE), the book describes all main aspects of polysaccharide science and technology (biology, enzymology, physics, chemistry, materials science and processing). Notations and concepts follow a uniform format throughout the whole work in order to create a valuable reference book on the field of polysaccharide science. Owing to the major importance of industry in the EPNOE, concrete applications are also described in detail.
  • Matrix metalloproteinases and their inhibitors in turnover and degradation of extracellular matrix / Sean E. Gill and William C. Parks -- Role of cysteine cathepsins in extracellular proteolysis / Dieter Brömme and Susan Wilson -- Cooperation between proteolysis and endocytosis in collagen turnover / Thomas H. Bugge and Niels Behrendt -- Pericellular proteolysis / Judith S. Bond, Timothy R. Keiffer, and Qi Sun -- Triple helicase activity and the structural basis of collagenolysis / Hideaki Nagase and Robert Visse -- Structural basis of extracellular matrix interactions with matrix metalloproteinases / Steven R. Van Doren -- The role of matrix metalloproteinases in cellular invasion and metastasis / Elena I. Deryugina and James P. Quigley -- Degradation of bone and the role of osteoclasts, bone lining cells and osteocytes / Vincent Everts and Paul Saftig -- Consequences of elastolysis / A. McGarry Houghton, Majd Mouded, and Steven D. Shapiro.
  • Extracellular matrix protocols. 2nd ed. 2009, Springer Protocols
    Retroviral delivery of ECM genes / Vitali alexeev and Olga Igoucheva -- Tissue-specific KO of ECM proteins / Mara Brancaccio, Emila Turco, and Emilio Hirsch -- Recombinant collagen trimers from insect cells and yeast / Johanna Myllyharju -- Eukaryotic expression and purification of recombinant extracellular matrix proteins carrying the Strep II tag / Neil Smyth ... [et al.] -- Preparation of recombinant fibronectin fragments for functional and structural studies / David Staunton ... [et al.] -- Quantitative determination of collagen cross-links / Nicholas C. Avery, Trevor J. Sims, and Allen J. Bailey -- ECM macromolecules : height-mapping and nano-mechanics using atomic force microscopy / Nigel W. Hodson, Cay M. Kielty, and Michael J. Sherratt -- Atomic force microscopy measurements of intermolecular binding forces / Gradimir N. Misevic, Yannis Karamanos, and Nikola J. Misevic -- Mass-mapping of ECM macromolecules by scanning transmission electron microscopy / Michael J. Sherratt ... [et al.] -- Chemical microscopy of biological samples by dynamic mode secondary ion mass spectrometry / Gradimir N. Misevic ... [et al.] -- ECM macromolecules : rotary shadowing and transmission electron microscopy / Michael J. Sherratt ... [et al.] -- Using self-assembled monolayers to pattern ECM proteins and cells on substrates / Emanuele Ostuni ... [et al.] -- Solid phase assays for studying ECM protein-protein interactions / A. Paul Mould -- Cell adhesion assays / Martin J. Humphries -- ECM degradation assays for analyzing local cell invasion / Vira V. Artym, Kenneth M. Yamada, and Susette C. Mueller -- Fluorescence-based assays for in vitro analysis of cell adhesion and migration / Paola Spessotto ... [et al.] -- Fibrin gel model for assessment of cellular contractility / Sharona Even-Ram -- Fluorescent labeling techniques for investigation of fibronectin fibrillogenesis (labeling fibronectin fibrillogenesis) / Roumen Pankov and Albena Momchilova -- Stromagenesis during tumorigenesis : characterization of tumor-associated fibroblasts and stroma-derived 3D matrices / Remedios Castelló-Cros and Edna Cukierman -- Tissue recombinants to study extracellular matrix targeting to basement membranes / Patricia Simon-Assmann ... [et al.] -- ECM and FGF-dependent assay of embryonic SMG epithelial morphogenesis : investigating growth factor/matrix regulation of gene expression during submandibular gland development / Ivan T. Rebustini and Matthew P. Hoffman -- Analyzing how cell adhesion controls mammary gland function by transplantation of embryonic mammary tissue from knockout mice / Teresa C.M. Klinowska and Charles H. Streuli -- Characterizing ECM production by cells encapsulated in hydrogels / Iossif A. Strehin and Jennifer H. Elisseeff -- Tissue engineering and cell-populated collagen matrices / Paul D. Kemp.
  • Extracellular DNA as Matrix Component in Microbial Biofilms / Wen-Chi Chiang and Tim Tolker-Nielsen -- Gene Transfer Agents and Defective Bacteriophages as Sources of Extracellular Prokaryotic DNA / Andrew S. Lang and J. Thomas Beatty -- Roles of Extracellular DNA in Bacterial Ecosystem / Katsuji Tani and Masao Nasu -- Stable Extracellular DNA: A Novel Substrate for Genetic Engineering that Mimics Horizontal Gene Transfer in Nature / Shinya Kaneko and Mitsuhiro Itaya -- Extracellular Nucleic Acids of the Marine Phototrophic Bacterium Rhodovulum sulfidophilum and Related Bacteria: Physiology and Biotechnology / Yo Kikuchi -- Systemic RNAi in C. elegans from the Viewpoint of RNA as Extracellular Signals / Lisa Timmons -- Circulating Nucleic Acids in Health and Disease / E. Y. Rykova, P. P. Laktionov and V. V. Vlassov -- Circulating MicroRNAs in Cancer / Patrick S. Mitchell and Muneesh Tewari -- Biology and Diagnostic Applications of Cell-Free Fetal Nucleic Acids in Maternal Plasma / Jason C. H. Tsang and Y. M. Dennis Lo -- The Biology of Circulating Nucleic Acids in Plasma and Serum (CNAPS) / P. B. Gahan and M. Stroun -- Modulation and Regulation of Gene Expression by CpG Oligonucleotides / Dennis Klinman and Sven Klaschik -- Immune Recognition of Nucleic Acids and Their Metabolites / Shohei Koyama, Shizuo Akira and Ken J. Ishii.
  • Bioavailability of flavanones / Mireia Urpi-Sarda ... [et al.] -- Bioavailability of dietary monomeric and polymeric flavan-3-ols / Alan Crozier, Michael N. Clifford, and Daniele Del Rio -- Anthocyanins : understanding their absorption and metabolism / Ronald L. Prior -- Bioavailability of flavonols and flavones / Mariusz Konrad Piskula, Kaeko Murota, and Junji Terao -- Bioavailability of isoflavones in humans / Aedín Cassidy, José Peñalvo, and Peter Hollman -- Dietary hydroxycinnamates and their bioavailability / Angelique Stalmach, Gary Williamson, and Mike Clifford -- Bioavailability of dihydrochalcones / Elke Richling -- Occurrence, bioavailability, and metabolism of resveratrol / Paola Vitaglione, Stefano Sforza, and Daniele Del Rio -- Bioavailability and metabolism of ellagic acid and ellagitannins / Mar Larrosa ... [et al.] -- Colon-derived microbial metabolites of dietary phenolic compounds / Anna-Marja Aura -- Synthesis of dietary phenolic metabolites and isotopically labeled dietary phenolics / Denis Barron ... [et al.] -- Interactions of flavan-3-ols within cellular signaling pathways / Cesar G. Fraga and Patricia I. Oteiza -- Flavonoids and vascular function / Ana Rodriguez-Mateos and Jeremy P.E. Spencer -- Effects of flavonoids on the vascular endothelium : what is known and what is next? / Antje R. Weseler and Aalt Bast -- Green tea flavan-3-ols and their role in protecting against Alzheimer's and Parkinson's disease pathophysiology / Orly Weinreb ... [et al.] -- Flavonoids and neuroinflammation / David Vauzour and Katerina Vafeiadou -- Effects of flavonoids on cognitive performance / Shibu M. Poulose and Barbara Shukitt-Hale -- Flavonoids and oral cancer / Thomas Walle -- Flavonoids and cancer-effects on dna damage / Piyawan Sitthiphong ... [et al.].
  • Principles of acid-base physiology -- Tools to use to diagnose acid-base disorders -- Metabolic acidosis: clinical approach -- Metabolic acidosis due to a deficit of NaHCO₃ -- Ketoacidosis -- Metabolic acidosis: acid gain types -- Metabolic alkalosis -- Respiratory acid-base disturbances -- Sodium and waterphysiology -- Hyponatremia -- Hypernatremia -- Polyuria -- Potassium physiology -- Hypokalemia -- Hyperkalemia -- Hyperglycemia.
  • Fluorescence spectroscopy 2008, ScienceDirect
    A method in enzymology for measuring hydrolytic activities in live cell environments / Beverly Z. Packard and Akira Komoriya -- Heterogeneity of fluorescence determined by the method of area-normalized time-resolved emission spectroscopy / N. Periasamy -- Multiparametric probing of microenvironment with solvatochromic fluorescent dyes / Andrey S. Klymchenko and Alexander P. Demchenko -- Site-selective red-edge effects / Alexander P. Demchenko -- Fluorescence approaches to quantifying biomolecular interactions / Catherine A. Royer and Suzanne F. Scarlata -- Forster resonance energy transfer measurements of transmembrane helix dimerization energetics / Mikhail Merzlyakov and Kalina Hristova -- Application of single-molecule spectroscopy in studying enzyme kinetics and mechanism / Jue Shi ... [et al.] -- Ultrafast fluorescence spectroscopy via upconversion : applications to biophysics / Jianhua Xu and Jay R. Knutson -- Use of fluorescence resonance energy transfer (FRET) in studying protein-induced DNA bending / Anatoly I. Dragan and Peter L. Privalov --Fluorescent pteridine probes for nucleic acid analysis / Mary E. Hawkins -- Single-molecule fluorescence methods for the analysis of RNA folding and ribonucleoprotein assembly / Goran Pljevaljčić and David P. Millar -- Using fluorophore-labeled oligonucleotides to measure affinities of protein-DNA interactions / Brian J. Anderson ... [et al.] -- Identifying small pulsatile signals within noisy data : a fluorescence application / Michael L. Johnson ... [et al.] -- Determination of zinc using carbonic anhydrase-based fluorescence biosensors / Rebecca Bozym ... [et al.] -- Instrumentation for fluorescence-based fiber optic biosensors / Richard B. Thompson ... [et al.]
  • This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.
  • Folding for the synapse 2011, Springer
    Folding for the Synapse addresses the current view on how protein folding and misfolding, controlled by molecular chaperones, contribute to synapse function and dysfunction. Molecular chaperones have been studied in relation to de novo protein folding, but there is increasing awareness that chaperone function is required for the regulation of protein dynamics when functioning physiologically as an isolated moiety or part of a protein complex. This book will introduce both important concepts of folding machineries and give examples of the biological relevance of further chaperone functions.
  • 1. Oxidative folding: coupling conformational folding and disulfide formation -- 2. The case of oxidative folding of ribonuclease A: factors impacting fold maturation of ER-processed proteins -- 3. Cystine knot folding in cyclotides -- 4. In vitro folding of single/double chain insulins and related proteins -- 5. Unfolding and refolding of disulfide proteins using the method disulfide scrambling -- 6. Oxidative protein folding with small molecules -- 7. Protein disulfide isomerase and the catalysis of oxidative protein folding -- 8. Allosteric disulfide bonds -- 9. The problem of expression of multidisulfide bonded recombinant proteins in E. coli -- 10. NMR-spectroscopic investigation of disulfide dynamics in unfolded states of proteins -- 11. A half-century of oxidative folding and protein disulfphide formation -- Index.
  • FOXO1, T-cell trafficking and immune responses / Florent Carrette, Stéphanie Fabre and Georges Bismuth -- FOXP3 and its role in the immune system / Chang H. Kim -- Molecular regulation of cellular immunity by FOXP3 / Alicia N. McMurchy ... [et al.] -- The biology of FOXP3 : a key player in immune suppression during infections, autoimmune diseases and cancer / Frances Mercer and Derya Unutmaz -- The cooperative roles of FOXC1 and FOXC2 in cardiovascular development / Tsutomu Kume -- FOXO proteins and cardiac pathology / Albert Wong and Elizabeth A. Woodcock -- The forkhead transcription factors play important roles in vascular pathology and immunology / Xiao-Feng Yang ... [et al.] -- Regulatory T cells, FOXP3 and atherosclerosis / Michal Entin-Meer, Arnon Afek and Jacob George -- FOXP genes, neural development, speech and language disorders / Hiroshi Takahashi, Kaoru Takahashi and Fu-Chin Liu -- Pathophysiological relevance of forkhead transcription factors in brain ischemia / Kohji Fukunaga and Norifumi Shioda -- New insights for FOXO and cell-fate decision in HIV infection and HIV associated neurocognitive disorder / Min Cui ... [et al.] -- FOXP3+ regulatory T-cells in chronic kidney disease : molecular pathways and clinical implications / Pascal Meier -- Transcriptional role of FOXO1 in drug resistance through antioxidant defense systems / Tomoko Goto and Masashi Takano -- FOXP3 expressing regulatory T-cells in allergic disease / Kayhan T. Nouri-Aria -- Human clinical phenotype associated with FOXN1 mutations / Claudio Pignata, Anna Fusco and Stefania Amorosi -- FOXL2 : at the crossroads of female sex determination and ovarian function / Bérénice A. Benayoun ... [et al.] -- FOXO transcription factors : from cell fate decisions to regulation of human female reproduction / Jan J. Brosens, Miranda S.C. Wilson and Eric W.-F. Lam -- The "O" class : crafting clinical care with FOXO transcription factors / Kenneth Maiese ... [et al.].
  • Introduction -- Characterization of Protein-Protein Interfaces, Considering Surface-Roughness and Local Shape -- Adhesion on Protein (and Other Rough Biomolecular) Surfaces.
  • Introduction -- Characterization of Protein-Protein Interfaces, Considering Surface-Roughness and Local Shape -- Adhesion on Protein (and Other Rough Biomolecular) Surfaces.
  • RNAissance / Juergen Brosius -- Nucleic Acids as Therapeutics / Saraswat Pushpendra, Pareek Arvind and Bhandari Anil -- Thio Effects as a Tool for Mechanistic Studies of the Cleavage of RNA Phosphodiester Bonds: The Chemical Basis / Mikko Ora, Tuomas Lönnberg and Harri Lönnberg -- Off-Target Effects and Safety Aspects of Phosphorothioate Oligonucleotides / Martina Stessl, Christian R. Noe and Johannes Winkler -- Oligonucleotide Conjugates: Rationale, Synthesis, and Applications / Yashveer Singh, Pierre Murat, Nicolas Spinelli and Eric Defrancq -- CRISPR: A Bacterial Immunity System Based on Small RNAs / Rolf Wagner and Ümit Pul -- Antisense Technology: From Unique Laboratory Tool to Novel Anticancer Treatments / Christine Di Cresce, Colin Way, Mateusz Rytelewski, Saman Maleki Vareki and Supritha Nilam, et al. -- Antisense-Mediated Reduction of Eukaryotic Noncoding RNAs / Xue-hai Liang, Timothy A. Vickers and Stanley T. Crooke -- Antisense Oligonucleotides in the Treatment of Malignant Gliomas / Gerardo Caruso, Mariella Caffo, Giuseppe Raudino, Federica Raudino and Mario Venza, et al. -- Natural Antisense Transcripts Mediate Regulation of Gene Expression / Marco Magistri and Mohammad Ali Faghihi -- Activation and Deactivation of Antisense and RNA Interference Function with Light / Jeane M. Govan and Alexander Deiters -- Ribozymes as Molecular Biology Reagents / Sanchita Bhadra, Arti Pothukuchy and Andrew Ellington -- RNA Technologies for Mitochondrial Genetics / André Dietrich, Katarzyna Rolle, Marta Gabryelska, Eliza Wyszko and Romain Val, et al. -- RNA-Based Therapies for Inherited Metabolic Diseases / Belén Pérez, Magdalena Ugarte and Lourdes R. Desviat -- RNA-Cleaving DNA Enzymes and Their Potential Therapeutic Applications as Antibacterial and Antiviral Agents / P. I. Pradeepkumar and Claudia Höbartner -- Hammerhead Ribozymes Against Virus and Viroid RNAs / Alberto Carbonell, Ricardo Flores and Selma Gago -- Suppression of Hepatitis C Viral Genome Replication with RNA-Cleaving Deoxyribozyme / Dal-Hee Min and Dong-Eun Kim -- Design of Synthetic shRNAs for Targeting Hepatitis C: A New Approach to Antiviral Therapeutics / Brian H. Johnston and Qing Ge -- The Diverse Active Sites in Splicing, Debranching, and MicroRNA Processing Around RNA Phosphodiester Bonds / Sourav Kumar Dey, Eduardo Paredes, Molly Evans and Subha R. Das -- MicroRNAs and Their Antagonists as Novel Therapeutics / Jeppe Vinther, Jakob Lewin Rukov and Noam Shomron -- microRNAs in Human Diseases and Viral Infections / Alessandra Mescalchin and Tobias Restle -- Dysregulation of MicroRNA Expression and Human Diseases? / To-Ha Thai -- Noncoding RNAs: Identification of Cancer-Associated MicroRNAs / Muller Fabbri and George A. Calin -- Targeting Non-coding RNAs for Cancer Therapy / Satya K. Kota and Savithri Balasubramanian -- MicroRNA Pathways in Drosophila / Geetanjali Chawla and Nicholas S. Sokol -- Viroids: The Smallest Known Infectious Agents Cause Accumulation of Viroid-Specific Small RNAs / Jaroslav Matoušek, Detlev Riesner and Gerhard Steger.
  • Fulltext MyiLibrary
  • Glycoproteomics in health and disease / Weston B. Struwe, Eoin F.J. Cosgrave, Jennifer C. Byrne, Radka Saldova, and Pauline M. Rudd -- Glyco-engineering of Fc Glycans to enhance the biological functions of therapeutic IgGs / T. Shantha Raju, David M. Knight, and Robert E. Jordan -- Bioinformatics databases and applications available for glycobiology and glycomics / René Ranzinger, Kai Maass, and Thomas Lütteke -- Lectin microarrays: Simple tools for the analysis of complex glycans / Lakshmi Krishnamoorthy and Lara K. Mahal -- The application of high throughput mass spectrometry to the analysis of glycoproteins / Sasha Singh, Morten Thaysen Andersen, and Judith Jebanathirajah Steen -- Solutions to the glycosylation problem for low- and high-throughput structural glycoproteomics / Simon J. Davis and Max Crispin -- Role of glycoproteins in virus-human cell interactions / Thomas A. Bowden and Elizabeth E. Fry.
  • Functional glycomics 2010, ScienceDirect
  • Functional lipidomics 2006, CRCnetBASE
  • Chromatin is the combination of DNA and proteins that make up the genetic material of chromosomes. It is essential for packaging DNA, regulation of gene expression, DNA replication and repair. The audience for this book includes newly established scientists, graduate students and scientists seeking detailed overviews of various aspects of chromatin structure and function to gain entry into this field. The chapters in this book give a strong, updated groundwork about the fundamentals of chromatin which are essential to understand epigenetics. Some the fundamentals that this book covers include the structure and biochemistry of chromatin and the enzyme complexes that manage it.
  • Pt. I. Protein structure. Amino acids ; Protein structure ; Purification and characterisation of proteins ; Protein sequencing -- Pt. II. Enzymes. Enzymes are biocatalysts ; Enzyme kinetics ; Haemoglobin and myoglobin: co-operativity ; Enzyme kinetics: special cases -- Pt. III. Special proteins. Prion proteins and prion diseases ; Immunoproteins ; Cell skeleton ; Motor proteins and movement ; Cell-cell interactions ; Aiding in folding: molecular chaperones and chaperonins -- Pt. IV. Membrane transport. Transport of proteins into mitochondria ; Intracellular protein transport ; Transport of solutes across membranes -- Appendix. A.1. Solutions for the exercise ; A.2. Short biographies of scientists mentioned in this book ; A.3. List of symbols ; A.4. Greek alphabet ; A.5. The genetic code ; A.6. Acronyms.
  • Section 1. GPCR trafficking. Therapeutic rescue of misfolded/mistrafficked mutants : automation-friendly high-throughput assays for identification of pharmacoperone drugs of GPCRs / David C. Smithson, Jo Ann Janovick, and P. Michael Conn ; Trafficking of the follitropin receptor / Alfredo Ulloa-Aguirre, James A. Dias, George Bousfield, Ilpo Huhtaniemi, and Eric Reiter ; Single-molecule imaging technique to study the dynamic regulation of GPCR function at the plasma membrane / B.E. Snaar-Jagalska, A. Cambi, T. Schmidt, and S. de Keijzer ; GPCR oligomerization and receptor trafficking / Richard J. Ward, Tian-Rui Xu, and Graeme Milligan ; [Beta]-arrestins and G protein-coupled receptor trafficking / Dong Soo Kang, Xufan Tian, and Jeffrey L. Benovic ; Tracking cell surface mobility of GPCRs using [alpha]-bungarotoxin-linked fluorophores / Saad Hannan, Megan E. Wilkins, Philip Thomas, and Trevor G. Smart ; Regulatory mechanism of G protein-coupled receptor trafficking to the plasma membrane : a role for mRNA localization / Kusumam Joseph, Eleanor K. Spicer, and Baby G. Tholanikunnel ; Dissecting trafficking and signaling of atypical chemokine receptors / Elena Borroni, Cinzia Cancellieri, Massimo Locati, and Raffaella Bonecchi -- Section 2. Trafficking motifs. Systematic and quantitative analysis of G protein-coupled receptor trafficking motifs / Carl M. Hurt, Vincent K. Ho, and Timothy Angelotti ; Identification of endoplasmic reticulum export motifs for G protein-coupled receptors / Guangyu Wu ; Amino acid residues of G-protein-coupled receptors critical for endoplasmic reticulum export and trafficking / Motonao Nakamura, Daisuke Yasuda, Nobuaki Hirota, Teruyasu Yamamoto, Satoshi Yamaguchi, Takao Shimizu, and Teruyuki Nagamune -- Section 3. GPCR oligomerization. G-protein-coupled heteromers : regulation in disease / Ivone Gomes, Achla Gupta, and Lakshmi A. Devi ; Hetero-oligomerization and specificity changes of G protein-coupled purinergic receptors : novel insight into diversification of signal transduction / Tokiko Suzuki, Kazunori Namba, Natsumi Mizuno, and Hiroyasu Nakata ; Bimolecular fluorescence complementation analysis of G protein-coupled receptor dimerization in living cells / Karin F.K. Ejendal, Jason M. Conley, Chang-Deng Hu, and Val J. Watts ; G protein-coupled receptor heterodimerization in the brain / Dasiel O. Borroto-Escuela, Wilber Romero-Fernandez, Pere Garriga, Francisco Ciruela, Manuel Narvaez, Alexander O. Tarakanov, Miklós Palkovits, Luigi F. Agnati, and Kjell Fuxe ; Experimental strategies for studying G protein-coupled receptor homo- and heteromerization with radioligand binding and signal transduction methods / Roberto Maggio, Cristina Rocchi, and Marco Scarselli ; Analysis of GPCR dimerization using acceptor photobleaching resonance energy transfer techniques / Marta Busnelli, Mario Mauri, Marco Parenti, and Bice Chini ; Techniques for the discovery of GPCR-associated protein complexes / Avais Daulat, Pascal Maurice, and Ralf Jockers ; Expression, purification, and analysis of G-protein-coupled receptor kinases / Rachel Sterne-Marr, Alison I. Baillargeon, Kevin R. Michalski, and John J.G. Tesmer ; Modern methods to investigate the oligomerization of glycoprotein hormone receptors (TSHR, LHR, FSHR) / Marco Bonomi and Luca Persani.
  • The protein local optimization program and g-protein-coupled receptors:loop restoration and applications to homology modeling -- Modeling active GPCR conformations -- Molecular modeling of adenosine receptors -- Predicting g-protein-coupled receptors families using differet physiochemical properties and pseudo amino acid composition -- Computational design and experimental characterization of GPCR segment models -- G-protein-coupled receptor interaction with small GTPases -- Quantification of receptor tyrosine kinase activiation and transactivation by G-protein-coupled receptors using spatial intensity distribution analysis (SplDA) -- NMR modeling for detection of small molecule binding to RGS4 -- Group II activators of g-protein signaling: monitoring the interaction of G[alpha] with G-protein motif in the intact cell -- Homogenous time-resolved fluorescence assay to probe folded G protein-coupled receptors -- cAMP biosensors applied in molecular pharmacological studies of G protein-coupled receptors -- Rhodospin-lipid intereactions studied by NMR -- Biasing the parathyroid hormone receptor: relating in vitro ligand efficacy to in vivo biological activity -- Application of Monte Carlo-based receptor ensemble docking to virtual screening for GPCR ligands -- From heptahelical bundle to hits from the haystack: structure-based virtual screening for GPCR ligands -- Kinetics and dynamics in the G protein-coupled receptor signaling cascade -- Magic angle spinning nuclear magnetic resonance spectroscopy fo G protein-coupled receptors -- Posttranslation modification of G protein-coupled receptor in relationship to biased agonism -- Protein S-nitrosylation measurement.
  • This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain. This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain.
  • G protein-coupled receptors : research and methods in the post-genomic era / Craig W. Stevens -- Examining the dynamic evolution of G protein-coupled receptors / Claudia Stäubert, Diana Le Duc, and Torsten Schöneberg -- Functional properties of virus-encoded and virus-regulated G protein-coupled receptors / Katja Spiess and Mette M. Rosenkilde -- Discovery and function of the very large G protein-coupled receptor / Perrin C. White and D. Randy McMillan -- Functional evolution of opioid family G protein-coupled receptors / Eyal Vardy, Craig W. Stevens, and Bryan L. Roth -- Investigation of G protein-coupled receptor function and regulation using antisense / Jonathon Willets and Craig Nash -- Signalosome profiling reveals allosteric interactions between G protein-coupled receptors / Katrin Altosaar ... [et al.] -- Use of optogenetic approaches to control intracellular signaling of G protein-coupled receptors / Olivia A. Masseck, Melanie D. Mark, and Stefan Herlitze -- Viral infection for G protein-coupled receptor expression in eukaryotic cells / Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi -- Using in vitro mutagenesis to characterize structure- function relationships in G protein-coupled receptors / Gregory W. Sawyer and Frederick J. Ehlert -- CRE Luc Mouse model for bioimaging ligand activation of G protein-coupled receptors / H. Gregory Polites and Kyriakos D. Economides -- Inducing conformational changes in G protein-coupled receptors by domain coupling / Hamiyet Unal and Sadashiva S. Karnik -- Rescue of defective G protein-coupled receptor function by intermolecular cooperation / Ashutosh Trehan ... [et al.] -- Design of super-arrestins for gene therapy of diseases associated with excessive signaling of G protein-coupled receptors / Vsevolod V. Gurevich and Eugenia V. Gurevich -- Importance of G protein-coupled receptor genetics in clinical medicine / Ramakrishna Devaki -- Pharmacogenomics of G protein-coupled receptor signaling and other pathways in essential hypertension / Pedro A. Jose ... [et al.] -- Exon sequencing of G protein-coupled receptor genes and perspectives for disease treatment / Angeliki Karamitri and Ralf Jockers -- Gene therapy using G protein-coupled receptors for the treatment of cardiovascular disease / Dario Leosco ... [et al.] -- Novel mechanisms of G protein-coupled receptor oligomer and ion channel Iiteractions in nociception / Ajay S. Yekkirala -- Discovery and characterization of leucine-rich repeat-containing G protein-coupled receptors / Marie-Isabelle Garcia, Valeria Fernandez-Vallone, and Gilbert Vassart -- Role of G protein-coupled receptors in adult neurogenesis / Katie M. Collette, Van A. Doze, and Dianne M. Perez.
  • G protein-coupled receptors (GPCRs) are heptahelical transmembrane receptors that convert extra-cellular stimuli into intra-cellular signaling, and ultimately into biological responses. Since GPCRs are natural targets for approximately 40% of all modern medicines, it is not surprising that they have been the subject of intense research. Notwithstanding the amount of data generated over the years, discovering ligands of these receptors with optimal therapeutic properties is not straightforward and has certainly been hampered for years by the lack of high-resolution structural information about these receptors.--Back cover.
  • GABA receptors. 3rd ed. 2007, Springer
  • Galanin 2010, Springer
    Galanin : a multitalented neuropeptide / T. Hökfelt and K. Tatemoto -- Galanin, galanin receptors, and drug targets / K. Mitsukawa, X. Lu, and T. Bartfai -- Galanin acts as a trophic factor to the central and peripheral nervous systems / S-A. Hobson ... [et al.] -- Galanin and spinal pain mechanisms : past, present, and future / Xiao-Jun Xu, Tomas Hökfelt, and Zsuzsanna Wiesenfeld-Hallin -- Galanin family of peptides in skin function / J.W. Bauer ... [et al.] -- Galanin in glia : expression and potential roles in the CNS / Helmut Butzkueven and Andrew L. Gundlach -- Galanin and the neuroendocrine axes / Istvan Merchenthaler -- Galanin and consummatory behavior : special relationship with dietary fat, alcohol, and circulating lipids / Jessica R. Barson, Irene Morganstern, and Sarah F. Leibowitz -- Neurochemical modulation of central cardiovascular control : the integrative role of galanin / Zaida Díaz Cabiale ... [et al.] -- Galanin impairs cognitive abilities in rodents : relevance to Alzheimer's disease / J.N. Crawley -- Neuroprotective role for galanin in Alzheimer's disease / Scott E. Counts ... [et al.] -- Galanin, galanin receptor subtypes, and depression-like behaviour / Eugenia Kuteeva ... [et al.] -- Galanin and epilepsy / J.T. Lerner, R. Sankar, and A.M. Mazarati -- Galanin and addiction / Marina R. Picciotto -- Galanin systems and ischemia : peptide and receptor plasticity in neurons and oligodendroglial precursors / Pei-Juan Shen and Andrew L. Gundlach -- The galanin system in cancer / I. Rauch and Barbara Kofler -- Galanin systems in non-mammalian vertebrates with special focus on fishes / Elsie Tachie Mensah, Helene Volkoff, and Suraj Unniappan -- Galanin-like peptide : neural regulator of energy homeostasis and reproduction / Catherine B. Lawrence and Gregory S. Fraley.
  • Gamma-Glutamyl Transpeptidases (g-GTs) are members of the N-terminal nucleophile hydrolase superfamily, enzymes that cleave the g-glutamyl amide bond of glutathione to liberate cysteinylglycine. The released g-glutamyl group can be transferred to water (hydrolysis) or to amino acids or short peptides (transpeptidation). g-GT plays a key role in the gamma glutamyl cycle by regulating the cellular levels of the antioxidant glutathione, hence it is a critical enzyme in maintaining cellular redox homeostasis.g-GT is upregulated during inflammation and in several human tumors, and it is involved in many physiological disorders related to oxidative stress, such as Parkinson's disease and diabetes. Furthermore, this enzyme is used as a marker of liver disease and cancer. This book covers current knowledge about the structure-function relationship of g-GTs and gives information about applications of g-GTs in different fields ranging from clinical biochemistry to biotechnology and biomedicine.
  • General principles of retrovirus vector design / Tammy Chang and Jiing-Kuan Yee -- Strategies for retrovirus-based correction of severe, combined immunodeficiency (SCID) / Alain Fischer, Salima Hacein-Bey-Abina, and Marina Cavazzana-Calvo -- Retrovirus and lentivirus vector design and methods of cell conditioning / Samantha Cooray, Steven J. Howe, and Adrian J. Thrasher -- Analysis of the clonal repertoire of gene-corrected cells in gene therapy / Anna Paruzynski ... [et al.] -- Developing novel lentiviral vectors into clinical products / Anna Leath and Kenneth Cornetta -- Lentivirus vectors in [beta]-thalassemia / Emmanuel Payen ... [et al.] -- Gene therapy for chronic granulomatous disease / Elizabeth M. Kang and Harry L. Malech -- Alternative splicing caused by lentiviral integration in the human genome / Arianna Moiani and Fulvio Mavilio -- Genotoxicity assay for gene therapy vectors in tumor prone mice / Eugenio Montini and Daniela Cesana -- Lentiviral hematopoietic cell gene therapy for X-linked adrenoleukodystrophy / Nathalie Cartier ... [et al.] -- Retroviral replicating vectors in cancer / Christopher R. Logg ... [et al.] -- Adeno-associated virus vectorology, manufacturing, and clinical applications / Joshua C. Grieger and R. Jude Samulski -- Gene delivery to the retina : from mouse to man / Jean Bennett, Daniel C. Chung, and Albert Maguire -- Generation of hairpin-based RNAi vectors for biological and therapeutic application / Ryan L. Boudreau and Beverly L. Davidson -- Recombinant adeno-associated viral vector reference standards / Philippe Moullier and Richard O. Snyder -- NIH oversight of human gene transfer research involving retroviral, lentiviral, and adeno-associated virus vectors and the role of the NIH Recombinant DNA Advisory Committee / Marina O'Reilly ... [et al.] -- Regulatory structures for gene therapy medicinal products in the European Union / Bettina Klug ... [et al.].
  • From microorganisms to humans, this volume provides an interdisciplinary overview of how genome integrity is maintained. It begins with DNA replication and continues with replicative DNA repair and pleiotropic protein interactions. It also reviews the cellular responses to radiation and genotoxic stress affecting whole genomes.
  • Coming full circle: Cyclin-dependent kinases as anti-cancer drug targets / R.P. Fisher -- Core and linker histone modifications involved in the DNA damage response / J.E. Chubb and S. Rea -- Chromatin assembly and signalling the end of DNA repair requires acetylation of histone H3 on lysine 56 / T. Costelloe and N.F. Lowndes -- Structure and function of histone H2AX / D.M. Pinto and A. Flaus -- The initiation step of eukaryotic DNA replication / H. Pospiech, F. Grosse and F.M. Pisani -- Non-coding RNAs: New players in the field of eukaryotic DNA replication / T. Krude -- Function of TopBP1 in Genome Stability / M. Sokka ... [et al.] -- Eukaryotic single-stranded DNA binding proteins: central factors in genome stability / S. Broderick ... [et al.] -- DNA polymerases and mutagenesis in human cancers / E. Crespan, A. Amoroso and G. Maga -- DNA polymerase [eta], a key protein in translesion synthesis in human cells / S. Cruet-Hennequart ... [et al.] -- The mitochondrial DNA polymerase in health and disease / W.C. Copeland -- Centromeres: assembling and propagating epigenetic function / M. Glynn ... [et al.] -- Nucleotide excision repair in higher eukaryotes: mechanism of primary damage recognition in global genome repair / N.I. Rechkunova and O.I. Lavrik -- Nonhomologous DNA end joining (NHEJ) and chromosomal translocations in humans / M.R. Lieber ... [et al.] -- Fluorescence-based quantification of pathway-specific DNA double-strand break repair activities: a powerful method for the analysis of genome destabilizing mechanisms / M. Bohringer and L. Wiesmuller -- Apoptosis: a way to maintain healthy individuals / C. Mondello and A.I. Scovassi -- The use of transgenic mice in cancer and genome stability research / S. Conmy and H.P. Nasheuer.
  • This book offers a valuable resource that allows students, researchers, educators and the general public to learn about proteomics and genomics. Chromosomes form the basis for our genetic heritage and are the code for protein synthesis. The Human Genome Map was presented in 2002, and the Proteome Sequence Map is currently being created by a global consortia initiative. Proteome and genome building blocks already form the basis of scientific research areas and shape major areas of the pharmaceutical and biomedical industries. The book provides background information on and our current understanding of these gene and protein areas, and explains in detail how cutting-edge science is using these resources to develop new medicines and new diagnostics for patient treatment and care. The book will benefit all students and researchers who need a good understanding of genomics and proteomics within the clinical field. Its content is also suitable for a broad readership, including those not specialized in this field. Dr. Marko-Varga is head of Div. Clinical Protein Science & Imaging at the Biomedical Center, Dept. of Measurement Technology and Industrial Electrical Engineering, Lund University. He's also Professor at the 1st Department of Surgery, Tokyo Medical University, Tokyo, Japan.
  • Ghrelin. 1st ed. 2012, ScienceDirect
    History to the discovery of ghrelin / Cyril Y. Bowers -- Orphan GPCRs and methods for identifying their ligands / Morikatsu Yoshida, Mikiya Miyazato, and Kenji Kangawa -- Purification of rat and human ghrelins / Masayasu Kojima, Hiroshi Hosoda, and Kenji Kangawa -- Variety of acyl modifications in mammalian ghrelins / Takanori Ida -- Determination of nonmammalian ghrelin / Hiroyuki Kaiya ... [et al.] -- Morphological analysis of ghrelin neurons in the hypothalamus / Haruaki Kageyama, Fumiko Takenoya, and Seiji Shioda -- High-performance liquid chromatography analysis of hypothalamic ghrelin / Takahiro Sato and Masayasu Kojima -- Standard sample collections for blood ghrelin measurements / Hiroshi Hosoda and Kenji Kangawa -- From ghrelin to ghrelin's O-acyl transferase / Jesus A. Gutierrez ... [et al.] -- Enzymatic characterization of GOAT, ghrelin O-acyltransferase / Hideko Ohgusu, Tomoko Takahashi, and Masayasu Kojima -- The study of ghrelin deacylation enzymes / Motoyasu Satou and Hiroyuki Sugimoto -- Synthesis of ghrelin : chemical synthesis and semisynthesis for large-scale preparation of modified peptides / Tomohiro Makino, Masaru Matsumoto, and Yoshiharu Minamitake -- Ghrelin O-acyltransferase assays and inhibition / Martin S. Taylor ... [et al.] -- Model-based evaluation of growth hormone secretion / Johannes D. Veldhuis and Daniel M. Keenan -- Ghrelin in the control of energy, lipid, and glucose metabolism / Kristy M. Heppner ... [et al.] -- Ghrelin and the vagus nerve / Yukari Date -- Measurement of AMP-activated protein kinase activity and expression in response to ghrelin / Chung Thong ... [et al.] -- Ghrelin and gastrointestinal movement / Naoki Fujitsuka ... [et al.] -- Ghrelin acylation by ingestion of medium-chain fatty acids / Yoshihiro Nishi, Hiroharu Mifune, and Masayasu Kojima -- Islet [beta]-cell ghrelin signaling for inhibition of insulin secretion / Katsuya Dezaki and Toshihiko Yada -- Rikkunshito as a ghrelin enhancer / Hiroshi Takeda ... [et al.] -- Thermogenic characterization of ghrelin receptor null mice / Ligen Lin and Yuxiang Sun -- Transgenic mice overexpressing ghrelin or ghrelin analog / Hiroyuki Ariyasu ... [et al.] -- Therapeutic potential of ghrelin in restricting-type anorexia nervosa / Mari Hotta ... [et al.] -- Clinical application of ghrelin for chronic respiratory diseases / Nobuhiro Matsumoto and Masamitsu Nakazato -- Clinical trial of ghrelin synthesis administration for upper GI surgery / Shuji Takiguchi ... [et al.].
  • Glioma signaling 2013, Springer
  • pt. B, 2008 ScienceDirect
  • Heparan sulfate proteoglycan in inflammation and angiogenesis / Lianchun Wang -- Heparan sulfate proteoglycans in infection / Allison H. Bartlett and Pyong Woo Park -- Glycans in cancer / Lubor Borsig -- Glycosaminoglycans in atherosclerosis and thrombosis / C.P. Vicente, J.A.P. Godoy, and C.C. Werneck -- Glycans in embryonic stem cells / Claire Pickford ... [et al.] -- Heparan sulfate proteoglycans in cancer therapy / Ezeddin Salem Gassar, Sherif A. Ibrahim, and Martin Götte -- Structure and biological activities of glycosaminoglycan analogs from marine invertebrates : new therapeutic agents? / Eliene O. Kozlowski ... [et al.]
  • Glycobiology 2010, ScienceDirect
  • Glycobiology protocols 2006, Springer Protocols
    Gene expression analysis of glycosylation-related genes by real-time polymerase chain reaction / Juan J. Garcia-Vallejo ... [et al.] -- Analysis of the glycodynamics of primary osteoblasts and bone cancer cells / Inka Brockhausen-Schutzbach, Xiaojing Yang, and Mark Harrison -- Micromethods for the characterization of lipid a core and o-antigen lipopolysaccharide / Cristina L. Marolda ... [et al.] -- Assays for a galactosyltransferase involved in the assembly of the O7 antigen repeat unit of escherichia coli / Inka Brockhausen-Schutzbach ... [et al.] -- High-throughput quantitation of metabolically labeled anionic glycoconjugates by scintillation proximity assay utilizing binding to cationic dyes / Karen J. Rees-Milton and Tassos P. Anastassiades -- Quantitative analysis of mucins in mucosal secretions using indirect ELISA / Pablo Argueso and Ilene K. Gipson -- Molecular dissection of the mouse zona pellucida: an electron microscopic perspective utilizing high-resolution colloidal-gold labeling methods / Frederick W. K. Kan -- Soluble adamantly glycosphingolipid analogs as probes of glycosphingolipid function / Clifford Lingwood ... [et al.] -- Diagnosis of Krabbe disease by use of a natural substrate / John W. Callahan and Marie-Anne Skomorowski -- In vitro assays of the functions of calnexin and calreticulin, lectin-chaperones of the endoplasmic reticulum / Breanna S. Ireland, Monika Niggemann, and David B. Williams -- Quantitative measurement of selectin-ligand interactions: assays to identify a sweet pill in a library of carbohydrates / Mark E. Beauharnois, Sriram Neelamegham, and Khushi L. Matta -- Sialic acid-binding specificities of siglecs / Nadine Bock and Soerge Kelm. The application of fluorophore-assisted carbohydrate -- Electrophoresis for the study of the dolichol pyrophosphate-linked oligosaccharides pathway in cell cultures and animal tissues / Ningguo Gao -- Partial purification of mannosylphosphorylundecaprenol synthase from micrococcus luteus: a useful enzyme for the biosynthesis of a variety of mannosylphosphorylpolyisoprenol products / Jeffrey S. Rush and Charles J. Waechter -- Assay of dolichyl-phospho-mannose synthase reconstituted in a lipid matrix / John S. Schutzbach -- O-mannosylation in mammalian cells / Tamao Endo and Hiroshi Manya -- Methods for analysis of unusual forms of o-glycosylation / Aleksandra Nita-Lazar and Robert S. Haltiwanger -- Structural analysis of naturally occurring sialic acids / Johannis P. Kamerling and Gerrit J. Gerwig -- Non-radioactive trans-sialidase screening assay / Silke Schrader and Roland Schauer -- Structural determination of o-glycans by tandem mass spectrometry / Catherine Robbe, Jean-Claude Michalski, and Calliope Capon -- Detailed structural analysis of n-glycans released from glycoproteins in SDS-page gel bands using HPLC combined with exoglycosidase array digestions / Louise Royle ... [et al.] -- Molecular modeling of glycosyltransferases / Anne Imberty ... [et al.] -- Galactoside_2,6-sialyltransferase (st6gal.i) and the sialyl_2,6-galactosyl-linkage in tissues and cell lines / Fabio Dall'Olio, Nadia Malagolini, and Mariella Chiricolo -- Visualizing intracellular distribution and activity of core 2 _(1,6)n-acetylglucosaminyltransferase-I (C2GNT-I) in living cells / Maelle Prorok-Hamon, Sylvie Mathieu, and Assou El-Battari --
  • Glycomics 2010, ScienceDirect
  • Glycomics methods and protocols 2009, Springer Protocols
    Analysis of N- and O-linked glycans from glycoproteins using MALTI-TOF mass spectrometry / Willy Morelle ... [et al.] -- Infrared multiphoton dissociation mass spectrometry for structural elucidation of oligosaccharides / Bensheng Li ... [et al.] -- Mass spectrometry of N-linked glycans / Parastoo Azadi and Christian Heiss -- Solid-phase permethylation for glycomic analysis / Yehia Mechref, Pilsoo Kang, and Milos V. Novotny -- Method for investigation of oligosaccharides using phenylhydrazine derivatization / Erika Lattová and Hélène Perreault -- Two-dimensional HPLC separation with reverse-phase-nano-LC-MS/MS for the characterization of glycan pools after labeling with 2-aminobenzamide / Manfred Wuhrer, Carolien A.M. Koeleman, and André M. Deelder -- Capillary lectin-affinity electrophoresis for glycan analysis / Kazuaki Kakehi and Mitsuhiro Kinoshita -- Analysis of methylated O-glycan alditols by reversed-phase nanoLC coupled CAD-ESI mass spectrometry / Franz-Georg Hanisch and Stefan Müller -- High-throughput and high-sensitivity nano-LC/MS and MS/MS for O-glycan profiling / Hasse Karlsson ... [et al.] -- Collision-induced dissociation tandem mass spectrometry for structural elucidation of glycans / Bensheng Li ... [et al.] -- The structural elucidation of glycosaminoglycans / Vikas Prabhakar, Ishan Capila, and Ram Sasisekharan -- Labelling heparan sulphate saccharides with chromophore fluorescence and mass tags for HLC and MS separations / Mark Skidmore ... [et al.] -- Small-scale enzymatic digestion of glycoproteins and proteoglycans for analysis of oligosaccharides by LC-MS and FACE gel electrophoresis / Ruby P. Estrella ... [et al.] -- Enrichment strategies for glycopeptides / Shigeyasu Ito, Ko Hayama, and Jun Hirabayashi -- Introductory glycosylation analysis using SDS-PAGE and peptide mass fingerprinting / Nicole Wilson, Raina Simpson, and Catherine Cooper-Liddell -- Characterization of N-linked glycosylation on recombinant glycoproteins produced in Pichia pastoris using ESI-MS and MALDI-TOF / Bing Gong ... [et al.] -- N-glycosylation site analysis of human platelet proteins by hydrazide affinity capturing and LC-MS/MS / Urs Lewandrowski and Albert Sickmann -- LC/MSn for glycoprotein analysis : N-linked glycosylation analysis and peptide sequencing of glycopeptides / Nana Kawasaki, Satsuki Itoh, and Teruhide Yamaguchi -- Detecting the "O-GlcNAcome" : detection, purification, and analysis of O-GlcNAc modified proteins / Natasha E. Zachara -- Preparation of a glycan library using a variety of glycosyltrasferases [i.e. glycosyltransferases] / Hiromi Ito ... [et al.] -- Data mining the PDB for glyco-related data / Thomas Lütteke and Claus-W. von der Lieth -- Saccharide microarrays for high-throughput interrogation of glycan-protein binding interactions / Andrew K. Powell, Zheng-liang Zhi, and Jeremy E. Turnbull -- Glocosaminoglycan characterization methodologies probing biomolecular interactions / Vikas Prabhakar, Ishan Capila, and Ram Sasisekharan -- Development and characterization of antibodies to carbohydrate antigens / Jamie Heimburg-Molinaro and Kate Rittenhouse-Olson -- Inhibition of glcosyltransferase activities as the basis for drug development / John Schutzbach and Inka Brockhausen -- Saturation transfer difference NMR spectroscopy as a technique to investigate protein-carbohydrate interactions in solution / Thomas Haselhorst, Anne-Christin Lamerz, and Mark von Izstein.
  • ER-bound steps in the biosynthesis of G protein-coupled receptors / Christian Nanoff and Michael Freissmuth -- Role of chaperones in G Protein coupled receptor signaling complex assembly / Denis J. Dupré, Maha M. Hammad, Patrick Holland and Jaime Wertman -- GPCR oligomerization: contribution to receptor biogenesis / Kathleen Van Craenenbroeck -- The functional size of GPCRs: monomers, dimers or tetramers? / Darlaine Pétrin and Terence E. Hébert -- Regulation of post-Golgi traffic of G protein-coupled receptors / Guangyu Wu -- Regulated GPCR trafficking to the plasma membrane: general issues and the CCR5 chemokine receptor example / Hamasseh Shirvani, Gabriel Gätà and Stefano Marullo -- Regulatory processes governing the cell surface expression of LH and FSH receptors / Deborah L. Segaloff -- Chaperone-mediated assembly of G protein complexes / Barry M. Willardson and Christopher M. Tracy -- Synthesis and assembly of G protein [beta][gamma] dimers: comparison of in vitro and in vivo studies / Jane Dingus and John D. Hildebrandt -- Preferential assembly of G-[alpha][beta][gamma] complexes directed by the [gamma] subunits / Janet D. Robishaw -- G Protein trafficking / Philip B. Wedegaertner -- Differential assembly of GPCR signaling complexes determines signaling specificity / Pascal Maurice, Abla Benleulmi-Chaachoua and Ralf Jockers -- GPCR and voltage-gated calcium channels (VGCC) signaling complexes / Christophe Altier -- Pharmacological chaperones correct misfolded GPCRs and rescue function: protein trafficking as a therapeutic target / Guadalupe Maya-Núñez, Alfredo Ulloa-Aguirre, Jo Ann Janovick and P. Michael Conn.
  • Basic physiology of the growth hormone/insulin-like growth factor (IGF)-axis / Laura M. Frago and Julie A. Chowen -- Experimental models for understanding the role of insulin-like growth factor-I and its receptor during development / Jose Luis Trejo, Eva Carro, and Deborah J. Burke -- Insulin-like growth factor binding proteins in development / Josef V. Silha and Liam J. Murphy -- Growth hormone, insulin-like growth factors, and the female reproductive system / Carolyn A. Bondy and Jian Zhou -- Skeletal muscle / Ana M. Fernandez and Derek LeRoith -- Cardiovascular and respiratory systems / Åsa Tivesten and Jörgen Isgaard -- Hematopoiesis / Wilson Savino, Salete Smaniotto, and Mireille Dardenne -- Brain development / Gregory J. Popken ... [et al.] -- Regulation of vertebrate sensory organ development : a scenario for growth hormone and insulin-like growth factors action / Amelia Diaz-Casares ... [et al.] -- Role of insulin-like growth factors in neuronal plasticity and neuroprotection / Ignacio Torres Alemán -- Aging and life span / Holly M. Brown-Borg -- Interactions of insulin-like growth factor-I and estrogen in the brain / Pablo Mendez, Gloria Patricia Cardona-Gomez, and Luis Miguel García-Segura -- Cancer / Adda Grimberg -- Genetic basis of proportional short stature / Ángel Campos-Barros, Karen E. Heath, Jesús Argente -- Future perspectives : from stem cells and IGF biology to the clinic / Yvan Arsenijevic.
  • "Offering a molecular perspective not available 20 years ago, [this edition] provides physical and chemical data on proteins, nucleic acids, lipids, and carbohydrates. Presented in an organized, concise, and simple-to-use format, this popular reference allows quick access to the most frequently used data. Covering a wide range of topics, from classical biochemistry to proteomics and genomics, it also details the properties of commonly used biochemicals, laboratory solvents, and reagents." --excerpts from book jacket.
  • Handbook of biomarkers 2010, Springer
    Technologies for discovery of biomarkers -- Biomarkers and molecular diagnostics -- Biomarkers for drug discovery and development -- Role of biomarkers in health care -- Biomarkers of cancer -- Biomarkers of disorders of the nervous system -- Biomarkers of cardiovascular disorders -- Biomarkers and personalized medicine -- Regulatory issues.
  • Handbook of glycomics. 1st ed. 2009, ScienceDirect
  • Handbook of metabolomics 2011, Springer Protocols
    Introduction to metabolomics / Teresa Whei-Mei Fan, Andrew N. Lane, and Richard M. Higashi -- Considerations of sample preparation for metabolomics investigation / Teresa Whei-Mei Fan -- Clinical aspects of metabolomics / Michael Bousamra II [and others] -- Structural mass spectrometry for metabolomics / Richard M. Higashi -- Metabolomic applications of inductively coupled plasma-mass spectrometry (ICP-MS) / Rob Henry and Teresa Cassel -- Principles of NMR for applications in metabolomics / Andrew N. Lane -- Novel NMR and MS approaches to metabolomics / Ian A. Lewis [and others] -- Metabolic flux analysis / Tae Hoon Yang -- Introduction to metabolic control analysis (MCA) / Maliackal Poulo Joy [and others] -- Application of tracer-based metabolomics and flux analysis in targeted cancer drug design / Marta Cascante, Vitaly Selivanov, and Antonio Ramos-Montoya -- Noninvasive fluxomics in mammals by nuclear magnetic resonance spectroscopy / Justyna Wolak [and others] -- Compositional analysis of phospholipids by mass spectrometry and phosphorus-31 nuclear magnetic resonance spectroscopy / M. Cecilia Yappert -- HumanCyc pathway-genome database and pathway tools software as tools for imaging and analyzing metabolomics data / Pedro Romero -- Metabolomics-edited transcriptomics analysis (META) / Teresa Whei-Mei Fan.
  • Iron -- NIckel -- Manganese -- Cobalt -- Molybdenum/Tungsten -- Copper -- Vanadium -- Zinc -- Calcium -- Magnesium.
  • Nucleic acids : structures, functions, and applications / Valentin V. Vlassov, Dimitrii V. Pyshnyi, and Pavel E. Vorobjev -- Isolation of viral DNA from cultures / Larry A. Hanson -- Isolation of viral RNA from cultures / Ling Zhao and Zhen F. Fu -- Preparation of viral samples for direct molecular applications / Grant S. Hansman -- Isolation of bacterial DNA from cultures / Dongyou Liu -- Isolation of bacterial RNA from cultures / Som Subhra Chatterjee and Trinad Chakraborty -- Isolation of plasmids / Armelle Bigot and Alain Charbit -- Preparation of bacterial samples for direct molecular applications / Knut Rudi ... [et al.] -- Isolation of nucleic acids from yeasts / Lakshman P. Samaranayake, Nipuna B. Parahitiyawa, and C. Jayampath Seneviratne -- Isolation of nucleic acids from filamentous fungi / Margaret E. Katz and Brian F. Cheetham -- Preparation of fungal specimens for direct molecular applications / Françoise Irlinger ... [et al.] -- Isolation of nucleic acids from protozoa / Vitaliano A. Cama and Lihua Xiao -- Isolation of nucleic acids from helminthes / Munehiro Okamoto and Akira Ito -- Isolation of nucleic acids from insects / Kun Yan Zhu -- Preparation of parasitic specimens for direct molecular applications / Huw Vaughan Smith and Rosely Angela Bergamin Nichols -- Isolation of nucleic acids from body fluids / Karen Page and Jacqueline Amanda Shaw -- Isolation of nucleic acids from soft tissues / Sergey Kovalenko -- Isolation of nucleic acids from hard tissues / Judith Weidenhofer and Jennifer A. Byrne -- Isolation of DNA from ancient samples / Paula F. Campos, Eske Willerslev, and M. Thomas P. Gilbert -- Preparation of forensic samples for direct molecular applications / Erin K. Hanson and Jack Ballantyne -- Isolation of nucleic acids from plants / Song Weining and Dongyou Liu -- Isolation of megabase-sized DNA fragments from plants / Meiping Zhang, Yaning Li, and Hong-Bin Zhang -- Purification of nucleic acids from gels / Chunfang Zhang and Theo Papakonstantinou.
  • Part I. RNA Synthesis - Enzymatic RNA Synthesis, Ligation and Modification -- Chapter 1. Enzymatic RNA Synthesis using Bacteriophage T7 RNA Polymerase / Heike Gruegelsiepe, Astrid Schön, Leif A. Kirsebom and Roland K. Hartmann, p. 3-21 -- Chapter 2. Production of RNAs with Homogeneous 5' and 3' Ends / Mario Mörl, Esther Lizano, Dagmar K. Willkomm and Roland K. Hartmann, p. 22-35 -- Chapter 3. RNA Ligation using T4 DNA Ligase / Mikko J. Frilander and Janne J. Turunen, p. 36-52 -- Chapter 4. T4 RNA Ligase / Tina Persson, Dagmar K. Willkomm and Roland K. Hartmann, p. 53-74 -- Chapter 5. Co- and Post-Transcriptional Incorporation of Specific Modifications Including Photoreactive Groups into RNA Molecules / Nathan H. Zahler and Michael E. Harris, p. 75-85 -- Chapter 6. 3'-Terminal Attachment of Fluorescent Dyes and Biotin / Dagmar K. Willkomm and Roland K. Hartmann, p. 86-94 -- Part I. RNA Synthesis - Chemical RNA Synthesis -- Chapter 7. Chemical RNA Synthesis, Purification and Analysis / Brian S. Sproat, p. 95-111 -- Chapter 8. Modified RNAs as Tools in RNA Biochemistry / Thomas E. Edwards and Snorri T. H. Sigurdsson, p. 112-129 -- Part II. Structure Determination - Molecular Biology Methods -- Chapter 9. Direct Determination of RNA Sequence and Modification by Radiolabeling Methods / Olaf Gimple and Astrid Schön, p. 132-150 -- Chapter 10. Probing RNA Structures with Enzymes and Chemicals In Vitro and In Vivo / Eric Huntzinger, Maria Possedko, Flore Winter, Hervé Moine, Chantal Ehresmann and Pascale Romby, p. 151-171 -- Chapter 11. Study of RNA-Protein Interactions and RNA Structure in Ribonucleoprotein Particles / Virginie Marchand, Annie Mougin, Agnès Méreau and Christiane Branlant, p. 172-204 -- Chapter 12. Terbium(III) Footprinting as a Probe of RNA Structure and Metal-binding Sites / Dinari A. Harris and Nils G. Walter, p. 205-213 -- Chapter 13. Pb²⁺-induced Cleavage of RNA / Leif A. Kirsebom and Jerzy Ciesiolka, p. 214-228 -- Chapter 14. In Vivo Determination of RNA Structure by Dimethylsulfate / Christina Waldsich and Renée Schroeder, p. 229-237 -- Chapter 15. Probing Structure and Binding Sites on RNA by Fenton Cleavage / Gesine Bauer and Christian Berens, p. 238-249 -- Chapter 16. Measuring the Stoichiometry of Magnesium Ions Bound to RNA / A. J. Andrews and Carol Fierke, p. 250-258 -- Chapter 17. Nucleotide Analog Interference Mapping and Suppression: Specific Applications in Studies of RNA Tertiary Structure, Dynamic Helicase Mechanism and RNA-Protein Interactions, p. 259-293 / Olga Fedorova, Marc Boudvillain, Jane Kawaoka and Anna Marie Pyle -- Chapter 18. Nucleotide Analog Interference Mapping: Application to the RNase P System / Simona Cuzic and Roland K. Hartmann, p. 294-318 -- Chapter 19. Identification and Characterization of Metal Ion Binding by Thiophilic Metal Ion Rescue / Eric L. Christian, p. 319-344 -- Chapter 20. Identification of Divalent Metal Ion Binding Sites in RNA/DNA-metabolizing Enzymes by Fe(II)-mediated Hydroxyl Radical Cleavage / Yan-Guo Ren, Niklas Henriksson and Anders Virtanen, p. 345-353 -- Chapter 21. Protein-RNA Crosslinking in Native Ribonucleoprotein Particles / Henning Urlaub, Klaus Hartmuth and Reinhard Lührmann, p. 354-373 -- Chapter 22. Probing RNA Structure by Photoaffinity Crosslinking with 4-Thiouridine and 6-Thioguanosine / Michael E. Harris and Eric L. Christian, p. 374-384 -- Part II. Structure Determination - Biophysical Methods -- Chapter 23. Structural Analysis of RNA and RNA-Protein Complexes by Small-angle X-ray Scattering / Tao Pan and Tobin R. Sosnick, p. 385-397 -- Chapter 24. Temperature-Gradient Gel Electrophoresis of RNA / Detlev Riesner and Gerhard Steger, p. 398-414 -- Chapter 25. UV Melting, Native Gels and RNA Conformation / Andreas Werner, p. 415-427 -- Chapter 26. Sedimentation Analysis of Ribonucleoprotein Complexes / Jan Medenbach, Andrey Damianov, Silke Schreiner and Albrecht Bindereif, p. 428-437 -- Chapter 27. Preparation and Handling of RNA Crystals / Boris François, Aurélie Lescoute-Phillips, Andreas Werner and Benoît Masquida, p. 438-452 -- Part II. Structure Determination - Fluorescence and Single Molecule Studies -- Chapter 28. Fluorescence Labeling of RNA for Single Molecule Studies / Filipp Oesterhelt, Enno Schweinberger and Claus Seidel, p. 453-474 -- Chapter 29. Scanning Force Microscopy and Scanning Force Spectroscopy of RNA / Wolfgang Nellen, p. 475-487 -- Part III. RNA Genomics and Bioinformatics - Fluorescence and Single Molecule Studies -- Chapter 30. Comparative Analysis of RNA Secondary Structure: 6S RNA / James W. Brow and J. Christopher Ellis, p. 490-512 -- Chapter 31. Secondary Structure Prediction / Gerhard Steger, p. 513-535 -- Chapter 32. Modeling the Architecture of Structured RNAs within a Modular and Hierarchical Framework, p. 536-545 / Benoît Masquida and Eric Westhof -- Chapter 33. Modeling Large RNA Assemblies using a Reduced Representation, p. 546-559 / Jason A. Mears, Scott M. Stagg and Stephen C. Harvey -- Chapter 34. Molecular Dynamics Simulations of RNA Systems / Pascal Auffinger and Andrea C. Vaiana, p. 560-576 -- Chapter 35. Seeking RNA Motifs in Genomic Sequences / Matthieu Legendre and Daniel Gautheret, p. 577-594 -- Chapter 36. Approaches to Identify Novel Non-messenger RNAs in Bacteria and to Investigate their Biological Functions: RNA Mining / Jörg Vogel, E. Gerhart and H. Wagner, p. 595-613 -- Chapter 37. Approaches to Identify Novel Non-messenger RNAs in Bacteria and to Investigate their Biological Functions: Functional Analysis of Identified Non-mRNAs / E. Gerhart, H. Wagner and Jörg Vogel, p. 614-642 -- Chapter 38. Experimental RNomics: A Global Approach to Identify Non-coding RNAs in Model Organisms / Alexander Hüttenhofer, p. 643-654 -- Chapter 39. Large-scale Analysis of mRNA Splice Variants by Microarray / Young-Soo Kwon, Hai-Ri Li and Xiang-Dong Fu, p. 655-663 -- Part IV. Analysis of RNA Function 665 - RNA-Protein Interactions in vitro -- Chapter 40. RNA-Protein Interactions in vitro / Steffen Schiffer, Sylvia Rösch, Bettina Späth, Markus Englert, Hildburg Beier and Anita Marchfelder, p. 666-675 -- Chapter 41. Biotin-based Affinity Purification of RNA-Protein Complexes / Zsofia Palfi, Jingyi Hui and Albrecht Bindereif, p. 676-693 -- Chapter 42. Immunoaffinity Purification of Spliceosomal and Small Nuclear Ribonucleoprotein Complexes / Cindy L. Will, Evgeny M. Makarov, Olga V. Makarova and Reinhard Lührmann, p. 694-709 -- Chapter 43. Northwestern Techniques for the Identification of RNA-binding Proteins from cDNA Expression Libraries and the Analysis of RNA-Protein Interactions / Ángel Emilio Martínez De Alba, Michela Alessandra Denti and Martin Tabler, p. 710-728 -- Part IV. Analysis of RNA Function 665 - RNA-Protein Interactions in vivo -- Chapter 44. Fluorescent Detection of Nascent Transcripts and RNA-binding Proteins in Cell Nuclei, p. 729-736 / Jennifer A. Geiger and Karla M. Neugebauer -- Chapter 45. Identification and Characterization of RNA-binding Proteins through Three-hybrid Analysis / Felicia Scott and David R. Engelke, p. 737-754 -- Chapter 46. Analysis of Alternative Splicing In Vivo using Minigenes / Yesheng Tang, Tatyana Novoyatleva, Natalya Benderska, Shivendra Kishore, Alphonse Thanaraj and Stefan Stamm, p. 755-782 -- Part IV. Analysis of RNA Function 665 - SELEX -- Chapter 47. Artificial Selection: Finding Function amongst Randomized Sequences / Ico de Zwart, Catherine Lozupone, Rob Knight, Amanda Birmingham, Mali Illangasekare, Vasant Jadhav, Michal Legiewicz, Irene Majerfeld, Jeremy Widmann and Michael Yarus, p. 783-806 -- Chapter 48. Aptamer Selection against Biological Macromolecules: Proteins and Carbohydrates / C. Stefan Vörtler and Maria Milovnikova, p. 807-839 -- Chapter 49. In Vivo SELEX Strategies / Thomas A. Cooper, p. 840-852 -- Chapter 50. In Vitro Selection against Small Targets / Dirk Eulberg, Christian Maasch, Werner G. Purschke and Sven Klussmann, p. 853-877 -- Chapter 51. SELEX Strategies to Identify Antisense and Protein Target Sites in RNA or Heterogeneous Nuclear Ribonucleoprotein Complexes / Martin Lützelberger, Martin R. Jakobsen and Jørgen Kjems, p. 878-894 -- Chapter 52. Gene Silencing Methods for Mammalian Cells: Application of Synthetic Short Interfering RNAs / Matthias John, Anke Geick, Philipp Hadwiger, Hans-Peter Vomlocher and Olaf Heidenreich, p. 896-909 -- Appendix. UV Spectroscopy for the Quantitation of RNA, p. 910-913.
  • Introduction to "a handbook of transcription factors" / T.R. Hughes -- An overview of prokaryotic transcription factors : a summary of function and occurrence in bacterial genomes / A.S. Seshasayee, K. Sivaraman and N.M. Luscombe -- A catalogue of eukaryotic transcription factor types, their evolutionary origin, and species distribution / M.T. Weirauch and T.R. Hughes -- Function and evolution of C2H2 zinc finger arrays / L. Stubbs, Y. Sun and D. Caetano-Anolles -- Homeodomain subtypes and functional diversity / T.R. Burglin -- Nuclear receptors : Small molecule sensors that coordinate growth, metabolism and reproduction / K. Pardee, A.S. Necakov and H. Krause -- Methods for analysis of transcription factor DNA-binding specificity in vitro / A. Jolma and J. Taipale -- Identification of transcription factor-DNA interactions in vivo / D.T. Odom -- How transcription factors identify regulatory sites in genomic sequence / Y. Field, E. Sharon and E. Segal -- Transcription factor binding sites and other features in human and Drosophila proximal promoters / C. Vinson, R. Chatterjee and P. Fitzgerald -- Interactions of transcription factors with chromatin / H. van Bakel -- Transcription factor effector domains / S. Frietze and P.J. Farnham -- Large-scale nuclear architecture and transcriptional control / J.M. Vaquerizas, A. Akhtar and N.M. Luscombe.
  • Harper's Illustrated biochemistry 2015 (30th ed.)., AccessMedicine
    29th ed., 2012 AccessMedicine
  • The HDL Handbook: Biological Functions to Clinical Implications brings laboratory research in HDL from bench to bedside in this needed resource for researchers and clinicians studying cholesterol, lipids, epidemiology, biochemistry, molecular medicine, and pathophysiology of cardiovascular diseases. In addition, researchers and clinicians working with an aging population, corporate researchers, post-doctorates; medical students and graduate students will find this publication useful because the scope of coverage includes basic science, genetics, epidemiology, and treatment of HDL cholesterol as well as potential targets to modify HDL cholesterol. Provides bench-to-bedside coverage of HDL with thorough coverage of basic science, genetics, epidemiology, and treatment Presents a complete update with six new chapters on the latest advances in HDL cholesterol research with international perspective New chapters on proteomics, clinical impact of LCAT in HDL metabolism, and an in-depth discussion of potential targets to modify HDL provide a translational reference for clinicians.
  • The Importance of Molecular Chaperones in Survival and Pathogenesis of the Malaria Parasite Plasmodium falciparum / Jude Przyborski -- General Structural and Functional Features of Molecular Chaperones / Adrienne L. Edkins and Aileen Boshoff -- The Role of Hsp70s in the Development and Pathogenicity of Plasmodium Species / Addmore Shonhai -- Role of the Hsp40 Family of Proteins in the Survival and Pathogenesis of the Malaria Parasite / Eva-Rachele Pesce, Alexander G. Maier and Gregory L. Blatch -- Role of Hsp90 in Plasmodium falciparum Malaria / Dea Shahinas and Dylan R. Pillai -- The Role of Parasite Heat Shock Proteins in Protein Trafficking and Host Cell Remodeling / Paul R. Gilson, Sarah C. Charnaud and Brendan S. Crabb -- Role of Heat Shock Proteins in Immune Modulation in Malaria / Evelyn Böttger and Gabriele Multhoff -- Establishment of Plasmodium falciparum Extracellular Compartments in its Host Erythrocyte / Alassane Mbengue, Laurence Berry and Catherine Braun-Breton -- Chaperones and Proteases of Plasmodium falciparum / Kaiyin Liu and Walid A. Houry -- Heat Shock Proteins as Targets for Novel Anti-Malarial Drugs / Manish Grover, Shweta Chaubey and Utpal Tatu -- Heat Shock Proteins of Malaria: What Do We Not Know, and What Should the Future Focus Be? / Addmore Shonhai and Gregory L. Blatch.
  • Hedgehog signaling protocols 2007, Springer Protocols
    Purifying the hedgehog protein and its variants / Darren P. Baker, Frederick R. Taylor, and R. Blake Pepinsky -- Application of sonic hedgehog to the developing chick limb / Eva Tieck and Cheryll Tickle -- Manipulation of hedgehog signaling in Xenopus by means of embryo microinjection and application of chemical inhibitors / Thomas Hollemann .. [et al.] -- Isolation of rat telencephalic neural explants to assay Shh GABAergic interneuron differentiation-inducing activity / Rina Mady and Jhumku D. Kohtz -- Genetic analysis of the vertebrate hedgehog-signaling pathway using muscle cell fate specification in the zebrafish embryo / Sudipto Roy -- Efficient manipulation of hedgehog/GLI signaling using retroviral expression systems / Maria Kasper ... [et al.] -- Cell surface marker and cell cycle analysis, hedgehog signaling, and flow cytometry / Krsting Detmer and Ronald E. Garner -- Detecting tagged hedgehog with intracellular and extracellular immunocytochemistry for functional analysis / Ainhoa Callejo, Luis Quijada, and Isabel Guerrero -- Confocal analysis of hedgehog morphogenetic gradient coupled with fluorescent in situ hybridization of hedgehog target genes / Armel Gallet and Pascal P. Thérond -- RNA in the hedgehog signaling pathway : pFRiPE, a vector for temporally and spatially controlled RNAi in Drosophila / Eric Marois and Suzanne Eaton -- Germline clone analysis for maternally acting Drosophila hedgehog components / Erica M. Selva and Beth E. Stronach -- Clone analysis of hedgehog signaling in Drosophila somatic tissues / Christing M. Bankers and Joan E. Hooper -- GAL4/UAS targeted gene expression for studying Drosophila hedgehog signaling / Denise Bussona nd Anne-Marie Pret -- Biochemical fractionation of Drosophila cells / Melanie Stegman and David Robbins -- Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway / Chao Ton and Jin Jiang -- Sequence analyses to study the evolutionary history and cis-regulatory elements of hedgehog genes / Ferenc Müller and Anne-Gaelle Borycki.
  • How the hedgehog outfoxed the crab: interference with HEDGEHOG-GLI signaling as anti-cancer therapy? / Ariel Ruiz i Altaba -- The patched receptor: switching on/off the hedgehog signaling pathway / Luis Quijada ... [et al.] -- Making a morphogenetic gradient / Henk Roelink -- Spatial and temporal regulation of hair follicle progenitors by hedgehog signaling / Anthony E. Oro -- Mode of PTCH1/Ptch1-associated tumor formation: insights from mutant Ptch1 mice / Heidi Hahn -- Basal cell carcinomas, hedgehog signaling, and the Ptch1+/- mouse / Ervin Epstein, Jr. -- GLI genes and their targets in epidermal development and disease / Fritz Aberger and Anna-Maria Frischauf -- Splitting hairs: dissecting the roles of Gli activator and repressor functions during epidermal development and disease / Pleasantine Mill and Chi-Chung Hui -- Shh expression in pulmonary injury and disease / Paul M. Fitch ... [et al.] -- Human correlates of GL13 function / Leslie G. Biersecker -- From oligodactyly to polydactyly: role of Shh and Gli3 in limb morphogenesis / Chin Chiang -- The genetics of Indian hedgehog / M. Elizabeth McCready and Dennis E. Bulman -- Sonic hedgehog signaling in craniofacial development / Dwight Cordero, Minal Tapadia and Jill A. Helms -- Important role of Shh controlling Gli3 functions during the dorsal-ventral patterning of the telencephalon / Jun Motoyama and Kazushi Aoto -- Regulation of early events in cell cycle progression by hedgehog signaling in CNS development and tumorigenesis / Anna Marie Kenney and David H. Rowitch -- Modulating the hedgehog pathway in diseases / Frederic J. de Sauvage and Lee L. Rubin -- Hedgehog signaling in endodermally derived tumors / Marina Pasca di Magliano and Matthias Hebrok.
  • History of heparin / T.W. Barrowcliffe -- Heparin biosynthesis / Pernilla Carlsson and Lena Kjellén -- Anticoagulant and antithrombotic mechanisms of heparin / Elaine Gray, John Hogwood, and Barbara Mulloy -- Standardisation of unfractionated and low-molecular-weight heparin / Elaine Gray -- Structure and physicochemical characterisation of heparin / Barbara Mulloy -- Case study: contamination of heparin with oversulfated chondroitin sulfate / Edward K. Chess ... [et al.] -- Low-molecular-weight heparins: differential characterization/physical characterization / Marco Guerrini and Antonella Bisio -- Heparin and heparan sulfate: analyzing structure and microheterogeneity / Zachary Shriver ... [et al.] -- Heparin in the prophylaxis and treatment of venous thromboembolism and other thrombotic diseases / Paolo Gresele, Chiara Busti, and Gloria Paganelli -- Adverse effects of heparin / S. Alban -- Neutralization of heparin activity / Menaka Pai and Mark A. Crowther -- Non-anticoagulant effects of heparin: an overview / Rebecca Lever and Clive P. Page -- Glycosaminoglycan and chemokine/growth factor interactions / Janis Shute -- Glycosaminoglycans and neuroprotection / B. Dudas and K. Semeniken -- Heparan sulphate: a heparin in miniature / J.T. Gallagher -- Heparin mimetics / Deirdre R. Coombe and Warren C. Kett -- Hyaluronic acid / Luigi Allegra, Sabrina Della Patrona, and Giuseppe Petrigni -- Semi-synthetic heparinoids / P. Oreste and G. Zoppetti -- Heparin-like entities from marine organisms / S. Colliec-Jouault, C. Bavington, and C. Belbarre-Ladrat.
  • Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh -- PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood -- High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina -- High-throughput insect cell protein expression applications / Mirjam Buchs ... [et al.] -- High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee -- The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens -- High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi -- Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio ... [et al.] -- Expression and purification of soluble His₆-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh -- High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery. High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt -- Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley -- Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath -- Flexi vector cloning / Paul G. Blommel ... [et al.] -- The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens -- The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley -- A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... [et al.] -- "System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart -- Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni²⁺-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery -- E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... [et al.].
  • Section 1. Histological and histochemical methods with light microscopy -- section 2. Cytological methods in electron microscopy -- section 3. Image quantification.
  • Histone deacetylase 1 / Dominique Meunier and Christian Seiser -- Biochemistry of multiprotein HDAC complexes / Alejandro Vaquero, Michael Scher, and Danny Reinberg -- The biology of HDAC3 / Edward Seto -- The biology of HDAC8, a unique class I histone deacetylase / David Waltregny and Vincent Castronovo -- Regulation of muscle gene expression by histone deacetylases / Timothy A. McKinsey and Eric N. Olson -- The class IIA histone deacetylases: functions and regulation / Herbert G. Kasler and Eric Verdin -- Histone deacetylases in the response to misfolded proteins / J. Andrew McKee and Tso-Pang Yao -- Evolution of sirtuins from archaea to vertebrates / Roy A. Frye -- Structure of the Sir2 family of NAD+-dependent histone/protein deacetylases / Kehao Zhao and Ronen Marmorstein -- The enzymology of Sir2 proteins / Margie T. Borra and John M. Denu -- The class III protein deacetylases: homologs of yeast Sir2P / Bjoern Schwer ... [et al.] -- HDAC inhibitors: discovery, development, and clinical impacts / Akihiro Ito, Norikazu Nishino, and Mitsuaki Yoshida -- Cell cycle targets of histone deacetylase inhibitors / Brian Gabrielli -- HDAC inhibitors: an emerging anticancer therapeutic strategy / Paul Kwon, Meier Hsu, Dalia Cohen, and Peter Atadja.
  • Excess of homocysteine, a product of the metabolism of the essential amino acid methionine, is associated with poor health, is linked to heart and brain diseases in general human populations, and accelerates mortality in heart disease patients. Neurological and cardiovascular abnormalities occur in patients with severe genetic hyperhomocysteinemia and lead to premature death due to vascular complications. Although it is considered a non-protein amino acid, studies over the past dozen years have discovered mechanisms by which homocysteine becomes a component of proteins. Homocysteine-containing proteins lose their normal biological function and become auto-immunogenic and pro-thrombotic. In this book, the author, a pioneer and a leading contributor to the field, describes up-to date studies of the biological chemistry of homocysteine-containing proteins, as well as pathological consequences and clinical implications of their formation. This is a comprehensive account of the broad range of basic science and medical implications of homocysteine-containing proteins for health and disease.
  • This textbook is specifically designed for upper-division undergraduate or graduate students in life science or pre-medical majors including dentistry or pharmacology, who are required to take a biochemistry or medical biochemistry course, but who are not necessarily biochemistry majors. The book adopts a unique approach to the topic compared with other biochemistry textbooks currently available, in that each biochemical subject is introduced by a human disease relating the biochemical principles to be developed in that chapter. The goal is to make biochemistry more meaningful to the student who is not normally shown the connection between biochemistry and medicine. This book: includes an abundance of figures; emphasizes human biochemistry; and introduces each chapter with a relevant disease or clinical relationship.
  • Nomenclature and molecular biology of the human sulfotransferase family / Rebecca Blanchard -- Structure and function of sulfotranserases / Jyrki Taskinen and W.H. Coughtrie -- PAPS and sulfoconjucation / Nancy B. Schwartz -- Sulfate conjugation: pharmacogenetics and pharmacogenomics / by Weinshilboum & Adjei -- Molecular cloning of the human cytosolic sulfotransferases / by C.N.Falany ... [et al.] -- Sulfotransferases in the human fetus and neonate / by Stanley, Coughtri and Hume -- Sulfation of thyroid hormones / by Monique H.A. Kester, Michael W.H. Coughtrie and Theo J. Vissor -- Estrogen sulfotransferase in breast cancer / by Pasqualini and Chetrite -- Sulfation of drugs / by Pacifici -- Human sult1a sulfotransferases / by Nadine Hempel ... [et al.] -- Hydroxysteroid sulfotransferases sult2a1, sult2b1a and sult2b1b / by Charles A. Strott -- Species differences in cytosol sulfotoransgerases / Kiyoshi Nagata, Miki Shimada, and Yasushi Yamazoe -- Activiation and inactivation of carconogens and mutagens by human sulfotranserases / by Hansruedi Glatt.
  • pt. B ScienceDirect
  • Anaerobic eukaryotes in pursuit of phylogenetic normality: the evolution of hydrogenosomes and mitosomes / W. Martin -- Protein import into hydrogenosomes and mitosomes / S. D. Dyall, P. Doležal -- Structure of the hydrogenosome / M. Benchimol -- Hydrogenosomes of anaerobic ciliates / J. H. P. Hackstein ... [et al.] -- Metabolism of trichomonad hydrogenosomes / I. Hrdý, J. Tachezy, M. Müller -- Hydrogenosomes of anaerobic chytrids: an alternative way to adapt to anaerobic environments / J. H. P. Hackstein ... [et al.] -- The proteome of T. vaginalis hydrogenosomes / K. Henze -- Hydrogenosome: the site of 5-nitroimidazole activation and resistance / J. Kulda, I. Hrdý -- Mitosomes in parasitic protists / J. Tachezy, O. Šmíd -- The mitochondrion-related organelle of Cryptosporidium parvum / J. S. Keithly -- Mitochondrial remnant in blastocystis / N. Yarlett, K. S. W. Tan -- Possible mitochondria-related organelles in poorly-studied "amitochondriate" eukaryotes / V. Hampl, A. G. B. Simpson.
  • Cardiovascular Risk Assessment -- The Metabolic Syndrome -- The Role of Imaging in Cardiovascular Risk Assessment -- Inherited Lipoprotein Disorders -- Advanced Lipid Testing -- Therapeutic Lifestyle Change for the Prevention and Treatment of Hyperlipidemia and Coronary Artery Disease -- Drug Therapy for Dyslipidemia -- Hypertriglyceridemia -- Isolated Low HDL -- Lipoprotein(a) -- HIV with Dyslipidemia -- Treatment of Dyslipidemia in Patients with Renal Insufficiency and End-Stage Renal Disease.
  • I domain integrins 2014, Springer
    "The integrin family is composed of 24 members and approximately ten years ago (2003) we published a book devoted to the nine I domain integrin subunits. In this second edition, I am pleased that most of the original authors have been able to contribute to the updated version. I domain containing integrins include collagen receptors and leukocyte receptors. In 2003 the knockout mouse phenotypes for all of the I domain integrins had not yet been published; they are now, and are summarized and discussed in this edition. Interestingly, a recent 10 integrin mutation in dogs has indicated that collagen-binding integrins in the musculoskeletal system might have much more severe phenotypes in larger animals/humans compared to the mild integrin phenotypes observed in collagen-binding integrin deficient mice. This finding is further discussed in the book. In the cancer field, the microenvironment is taking center stage, and here collagen receptors on fibroblasts are predicted to play important roles in paracrine signaling, in regulating tissue stiffness and matrix remodeling. New technologies, new mouse models in combination with analyses of I integrins in larger animals/humans are thus predicted to increase our knowledge about this group of receptors. With this in mind we look forward to another 10 years of research with I domain integrins."--Publisher's wevsite.
  • Section 1. Viruses, microbes, and parasites. Microbial cells analysis by atomic force microscopy / David Alsteens ; Understanding parasite transmission through imaging approaches / Mirko Singer and Freddy Frischknecht ; Multiphoton microscopy applied for real-time intravital imaging of bacterial infections in vivo / Ferdinand X. Choong ... [et al.] ; Analysis of virus entry and cellular membrane dynamics by single particle tracking / Helge Ewers and Mario Schelhaas ; Imaging of live malaria blood stage parasites / Christof Grüring and Tobias Spielmann ; Escherichia coli K1 invasion of human brain microvascular endothelial cells / Lip Nam Loh and Theresa H. Ward -- Section 2. Disease states. Real-time imaging after cerebral ischemia : model systems for visualization of inflammation and neuronal repair / Pierre Cordeau and Jasna Kriz ; In vivo real-time visualization of leukocytes and intracellular hydrogen peroxide levels during a zebrafish acute inflammation assay / Luke Pase, Cameron J. Nowell, and Graham J. Lieschke ; Imaging protein oligomerization in neurodegeneration using bimolecular fluorescence complementation / Federico Herrera, Susana Gonçalves, and Tiago Fleming Outeiro ; 3D/4D functional imaging of tumor-associated proteolysis : impact of microenvironment / Kamiar Moin ... [et al.] -- Section 3. Other techniques. Live cell imaging in live animals with fluorescent proteins / Robert M. Hoffman ; Protein activation dynamics in cells and tumor micro arrays assessed by time resolved Förster resonance energy transfer / Véronique Calleja, Pierre Leboucher, Banafshe ́Larijani ; Imaging stem cell differentiation for cell-based tissue repair / Zhenghong Lee ... [et al.] ; Understanding the initiation of B cell signaling through live cell imaging / Angel M. Davey ... [et al.] ; A quantitative method for measuring phototoxicity of a live cell imaging microscope / Jean-Yves Tinevez ... [et al.] ; High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines / Davide Danovi ... [et al.] ; High-throughput screening in primary neurons / Punita Sharma ... [et al.] ; Live imaging fluorescent proteins in early mouse embryos / Panagiotis Xenopoulos, Sonja Nowotschin, and Anna-Katerina Hadjantonakis ; Methods for three-dimensional analysis of dendritic spine dynamics / Enni Bertling ... [et al.] ; Imaging cell competition in Drosophila imaginal discs / Shizue Ohsawa ... [et al.] ; Live cell imaging of the oviduct / Sabine Kölle -- Computational quantification of fluorescent leukocyte numbers in zebrafish embryos / Felix Ellett and Graham J. Lieschke -- Four-dimensional tracking of lymphocyte migration and interactions in lymph nodes by two-photon microscopy / Masahiro Kitano and Takaharu Okada.
  • Monitoring cytoskeletal dynamics in living neurons using fluorescence photoactivation / Anne Gauthier-Kemper ... [et al.] -- Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays / Roy Edward -- Methods to measure actin treadmilling rate in dendritic spines / Mikko Koskinen, Enni Bertling, and Pirta Hotulainen -- Live-cell imaging of clathrin coats / Comert Kural and Tom Kirchhausen -- Imaging the mitotic spindle / Paul S. Maddox ... [et al.] -- Modeling of spatial intracellular signaling events in neurons / Wendy C. Wenderski and Susana R. Neves -- Imaging chromosome dynamics in meiosis in plants / Moira J. Sheehan and Wojciech P. Pawlowski -- Analyzing lysosomes in live cells / Paul R. Pryor -- Detection and quantification of biomolecular association in living cells using single-molecule microscopy / Mario Brameshuber and Gerhard J. Schütz -- Visualizing cardiac ion channel trafficking pathways / James W. Smyth and Robin M. Shaw -- Live cell imaging of the cytoskeleton / Eve G. Stringham ... [et al.] -- Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms / Naoki Watanabe -- Analysis of cell dispersion and migration by video-microscopy / Bonnomet Arnaud ... [et al.] -- Real time qualitative and quantitative GLUT4 translocation assay / Maleppillil Vavachan Vijayakumar and Manoj Kumar Bhat -- Advanced imaging of cellular signaling events / Marek Cebecauer, Jana Humpolíčková, and Jerémie Rossy -- Dynamic imaging of homo-FRET in live cells by fluorescence anisotropy microscopy / Subhasri Ghosh ... [et al.] -- Time-resolved luminescence resonance energy transfer imaging of protein-protein interactions in living cells / Harsha E. Rajapakse and Lawrence W. Miller -- Imaging of corticosteroid receptors in live cells / Mayumi Nishi -- Investigating second messenger signaling in vivo / Rüdiger Rudolf, Mathias Hafner, and Marco Mongillo -- Probes for intracellular RNA imaging in live cells / Philip J. Santangelo ... [et al.] -- Imaging the glycome in living systems / Boyangzi Li, Feiyan Mock, and Peng Wu -- Intracellular magnesium detection by fluorescent Indicators / Valentina Trapani ... [et al.] -- Illuminating mobile zinc with fluorescence : from cuvettes to live cells and tissues / Zhen Huang and Stephen J. Lippard -- Quantitative fluorescent live cell Imaging of intracellular Ca2+ and H+ ions in malaria parasites / Petra Rohrbach.
  • Laser-induced radiation microbeam technology and simultaneous real-time fluorescence imaging in live cells / Stanley W. Botchway ... [et al.] -- A cell biologist's guide to high resolution imaging / Graeme Ball ... [et al.] -- Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo / André E.P. Bastos ... [et al.] -- Detecting and tracking nonfluorescent nanoparticle probes in live cells / Gufeng Wang and Ning Fang -- Fluorescence lifetime microscopy of tumor cell invasion, drug delivery, and cytotoxicity / Gert-Jan Bakker ... [et al.] -- Measuring membrane protein dynamics in neurons using fluorescence recovery after photobleach / Inmaculada M. González-González ... [et al.] -- Fluorescent speckle microscopy in cultured cells / Marin Barisic, António J. Pereira, and Helder Maiato -- Green-to-red photoconvertible mEosFP-aided live imaging in plants / Jaideep Mathur ... [et al.] -- Methods for cell and particle tracking / Erik Meijering, Oleh Dzyubachyk, and Ihor Smal -- Correlative light-electron microscopy : a potent tool for the imaging of rare or unique cellular and tissue events and structures / Alexander A. Mironov and Galina V. Beznoussenko -- Optical techniques for imaging membrane domains in live cells (live-cell palm of protein clustering) / Dylan M. Owen ... [et al.] -- Single live cell topography and activity imaging with the Shear-Force-Based Constant-Distance Scanning Electrochemical Microscope / Albert Schulte, Michaela Nebel, and Wolfgang Schuhmann -- Visualization of TGN-endosome trafficking in mammalian and drosophila cells / Satoshi Kametaka and Satoshi Waguri -- Live cell imaging with chemical specificity using dual frequency CARS microscopy / Iestyn Pope ... [et al.] -- Imaging intracellular protein dynamics by Spinning Disk Confocal microscopy / Samantha Stehbens ... [et al.] -- Visualizing dynamic activities of signaling enzymes using genetically encodable FRET-based biosensors : from designs to applications / Xin Zhou, Katie J. Herbst-Robinson, and Jin Zhang -- Live-cell imaging of Aquaporin-4 supramolecular assembly and diffusion / A.S. Verkman, Andrea Rossi, and Jonathan M. Crane -- Coiled-coil tag-probe labeling methods for live-cell imaging of membrane receptors / Yoshiaki Yano ... [et al.] -- Monitoring protein interactions in living cells with fluorescence lifetime imaging microscopy / Yuansheng Sun ... [et al.] -- Open source tools for fluorescent imaging / Nicholas A. Hamilton -- Nanoparticle PEBBLE sensors in live cells / Yong-Eun Koo Lee and Raoul Kopelman.
  • Energy systems for ATP regeneration in cell-free protein synthesis reactions / Kara A. Calhoun and James R. Swartz -- Continuous-exchange protein-synthesizing systems / Vladimir A. Shirokov ... [et al.] -- Cell-free production of integral membrane proteins on a preparative scale / Christian Klammt ... [et al.] -- SIMPLEX : a novel method for high-throughput construction and screening of protein libraries / Suang Rungpragayphan, Tsuneo Yamane, and Hideo Nakano -- Methods for high-throughput materialization of genetic information based on the wheat germ cell-free expression system / Tatsuya Sawasaki ... [et al.] -- Exogenous protein expression in Xenopus oocytes : basic procedures / Elena Bossi ... [et al.] -- In vitro translation to study HIV protease activity / Zene Matsuda ... [et al.] -- The protein truncation test in mutation detection and molecular diagnosis / Oliver Hauss and Oliver Muller -- Creation of novel enantioselective lipases by SIMPLEX / Yuichi Koga, Tsuneo Yamane, and Hideo Nakano -- In vitro transcription and translation coupled with two-dimensional electrophoresis for bacterial proteome analysis / Nathalie Norais ... [et al.] -- In vitro screen of bioinformatically selected Bacillus anthracis vaccine candidates by coupled transcription, translation, and immunoprecipitation analysis / Orit Gat, Haim Grosfeld, and Avigdor Shafferman -- Functional expression of Type 1 rat GABA transporter in microinjected Xenopus laevis oocytes / Stefano Giovannardi ... [et al.] -- Production of protein for nuclear magnetic resonance study using the wheat germ cell-free system / Toshiyuki Kohno and Yaeta Endo.
  • Informatics in proteomics 2005, CRCnetBASE
    Chapter 1. The Promise of Proteomics: Biology, Applications, and Challenges / Paul D. Wagner and Sudhir Srivastava -- Chapter 2. Proteomics Technologies and Bioinformatics / Sudhir Srivastava and Mukesh Verma -- Chapter 3. Creating a National Virtual Knowledge Environment.
  • The role of inositol and the principles of labelling, extraction, and analysis of inositides in mammalian cells / Christopher J. Barker and Per-Olof Berggren -- HPLC separation of inositol polyphosphates / Christopher J. Barker, Christopher Illies, and Per-Olof Berggren -- Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in mammalian cells / James C. Otto and John D. York -- A femtomole-sensitivity mass assay for inositol hexakisphosphate / Andrew J. Letcher, Michael J. Schell, and Robin F. Irvine -- Synthesis of InsP₇ by the inositol hexakisphosphate kinase 1 (IP6K1) / Cristina Azevedo ... [et al.] -- Protein pyrophosphorylation by diphosphoinositol pentakisphosphate (InsP₇) / J. Kent Werner Jr., Traci Speed, and Rashna Bhandari -- Synthesis and nonradioactive micro-analysis of diphosphoinositol phosphates by HPLC with postcolumn complexometry / Hongying Lin, Karsten Lindner, and Georg W. Mayr -- Diphosphosinositol polyphosphates and energy metabolism : assay for ATP/ADP ratio / Andreas Nagel ... [et al.] -- Isolation of inositol 1,4,5-triphosphate receptor-associating proteins and selective knockdown using RNA interference / Akihiro Mizutani ... [et al.] -- Phosphoinositide-specific phospholipase C [beta]1 signal transduction in the nucleus / Roberta Fiume ... [et al.] -- Methods to assess changes in the pattern of nuclear phosphoinositides / Nullin Divecha -- Measurement of phosphoinositide 3-kinase products in cultured mammalian cells by HPLC / Frank T. Cooke -- Phosphoinositide analysis by liquid chromatography-mass spectrometry / Trevor R. Pettitt -- Imaging phosphoinositide dynamics in living cells / Anne Wuttke, Olof Idevall-Hagren, and Anders Tengholm.
  • Identification of cholinergic synaptic transmission in the insect nervous system / Steeve Hervé Thany, Hélène Tricoire-Leignel, and Bruno Lapied -- The evolution of pentameric ligand-gated ion channels / Joseph A. Dent -- Diversity of insect nicotinic acetylcholine receptor subunits / Andrew K. Jones and David B. Sattelle -- Identification of critical elements determining toxins and insecticide affinity, ligand binding domains, and channel properties / Hélène Tricoire-Leignel and Steeve Hervé Thany -- Electrophysiological studies and pharmacological properties of insect native nicotinic acetylcholine receptors / Steeve Hervé Thany -- Characterisation of insect nicotinic acetylcholine receptors by heterologous expression / Neil S. Millar and Stuart J. Lansdell -- Neonicotinoid insecticides : historical evolution and resistance mechanisms / Steeve Hervé Thany -- Ecotoxicity of neonicotinoid insecticides to bees / Axel Decourtye and James Devillers -- State of the art on insect nicotinic acetylcholine receptor function in learning and memory / Monique Gauthier.
  • Terpene indole alkaloid biosynthesis / Sarah E. O'Connor, Elizabeth McCoy -- Pathways to diverse ergot alkaloid profiles in fungi / Daniel G. Panaccione, Christopher L. Schardl, Christine M. Coyle -- Compartmentalization of plant secondary metabolism / Nailish Samanani, Peter J. Facchini -- Comparative genomics of the shikimate pathways in Arabidopsis, Populus trichocarpa and Oryza sativa : shikimate pathway gene family structure and identification of candidates for missing links in pehnylalanine biosynthesis / Björn Hamberger ... [et al.] -- Tomato glandular trichomes as a model system for exploring evolution of specialized metabolism in a single cell / Eyal Fridman ... [et al.] -- Anacardic acid biosynthesis and bioactivity / David J. Schultz, Nalinie S. Wickramasinghe, Carolyn M. Klinge -- Molecular and biochemical investigations of sorgoleone biosynthesis / Daniel Cook ... [et al.] -- Biosynthesis of terpenophenolic metabolites in hop and cannabis / Jonathan E. Page, Jana Nagel -- Engineering pathway enzymes to understand the function and evolution of sterol structure and activity / Pruthvi Jayasimha ... [et al.] -- Methylation and demethylation of plant signaling molecules / Yue Yang ... [et al.] -- Recent advances in auxin biosynthesis and conjugation / Amber Kei Bowers, Yunde Zhao -- Auxin biology and biosynthesis / Jessica Calio, Yuen Yee Tam, Jennifer Normanly -- Translational opportunities in plant biochemistry / Cecilia A. McIntosh.
  • Integrins 2007, ScienceDirect
  • Intermediate filaments 2006, Springer
    Desmin and other intermediate filaments in normal and diseased muscle / Denise Paulin and Zhigang Xue -- Intermediate filaments in astrocytes in health and disease / Milos Pekny and Ulrika Wilhelmsson -- Neuronal intermediate filaments and neurodegenerative diseases / Gee Y. Ching and Ronald K.H. Liem -- Neurofilaments: phosphorylation and signal transduction / Sashi Kesavapany, Richard H. Quarles and Harish C. Pant -- Keratin intermediate filaments and diseases of the skin / E. Birgitte Lane -- The keratin K6 minifamily of genes / Manuel Navarro -- Transcriptional regulation of keratin gene expression / Miroslav Blumenberg -- Simple epithelial keratins: expression, function and disease / M. Llanos Casanova, Ana Bravo and José L. Jorcano -- Keratins as targets in and modulators of liver diseases / Kurt Zatloukal ... [et al.] -- The search for specific keratin functions: the case of keratin K10 / Mirentxu Santos ... [et al.].
  • Site-2 protease at ten -- Signal peptide peptidases -- GXGD-type intramembrane proteases -- Rhomboid intramembrane serine proteases -- Proteases of the Rhomboid family in the yeast Saccharomyces cerevisiae -- [gamma]-Secretase and Alzheimer's disease -- [gamma]-Secretase mediated proteolysis: at the cutting edge of notch signaling.
  • ch. 1. Introduction, p. 1-10 -- ch. 2. Biochemical Data: Analysis and Management, p. 11-40 -- ch. 3. Biochemical Exploration: Internet Resources, p. 41-52 -- ch. 4. Molecular Graphics: Visualization of Biomolecules, p. 53-71 -- ch. 5. Biochemical Compounds: Structure and Analysis, p. 73-105 -- ch. 6. Dynamic Biochemistry: Biomolecular Interactions, p. 107-121 -- ch. 7. Dynamic Biochemistry: Enzyme Kinetics, p. 123-146 -- ch. 8. Dynamic Biochemistry: Metabolic Simulation, p. 147-163 -- ch. 9. Genomics: Nucleotide Sequences and Recombinant DNA, p. 165-182 -- ch. 10. Genomics: Gene Identification, p. 183-208 -- ch. 11. Proteomics: Protein Sequence Analysis, p. 209-231 -- ch. 12. Proteomics: Prediction of Protein Structures, p. 233-268 -- ch. 13. Phylogenetic Analysis, p. 269-284 -- ch. 14. Molecular Modeling: Molecular Mechanics, p. 285-314 -- ch. 15. Molecular Modeling: Protein Modeling, p. 315-341 -- Appendices.
  • The Continuing Fascination with Jaks and Stats: An Introduction / Thomas Decker and Mathias Müller -- Regulation of JAKs: Insights Gleaned from the Functional Protein Domains / Claude Haan, Daniela Ungureanu, Tuija Pekkala, Olli Silvennoinen and Serge Haan -- Nuclear Functions of the Janus Kinases / Mark A. Dawson and Andrew J. Bannister -- Self-association of STAT Proteins from Monomers to Paracrystals / Mathias Droescher and Uwe Vinkemeier -- How Stats Interact with the Molecular Machinery of Transcriptional Activation / Amanda M. Jamieson, Matthias Farlik and Thomas Decker -- Nongenomic Functions of STAT3 / Daniel J. Gough, Pravin Sehgal and David E. Levy -- Evolution of Jak and Stat Proteins / Claus Vogl, Priyank Shukla and Ingo Ebersberger -- JAK/STAT and Chromatin Regulation in Drosophila / Kriti Gaur and Willis X. Li -- JAK/STAT Signaling and Invertebrate Immune Responses / Feng Zhou and Hervé Agaisse -- Stat5 as a Hematopoietic Master Regulator for Differentiation and Neoplasia Development / Harini Nivarthi, Katrin Friedbichler and Richard Moriggl -- Regulation of Dendritic Cell Development by STATs / Haiyan S. Li and Stephanie S. Watowich. STAT Transcription Factors: Controlling All Aspects of NK Cell Biology / Eva Maria Putz, Eva Zebedin and Veronika Sexl -- Function of JAKs and STATs in Lymphocytes: Bench to Bedside / Alejandro Villarino, Xiang-Ping Yang, Kiyoshi Hirahara, Yuka Kanno and John J. O' Shea, et al. -- The Role of Janus Kinases in Hematopoietic Malignancies / Damla Olcaydu and Robert Kralovics -- Activation of Janus Kinases During Tumorigenesis / Jeffrey W. Schmidt and Kay-Uwe Wagner -- STAT1 and STAT3 Transcription Factors in Inflammation-associated Colon Cancer / Paulina Rampetsreiter and Robert Eferl -- Universal and Specific Functions of STAT3 in Solid Tumours / Lidia Avalle, Gabriella Regis and Valeria Poli -- Impact of STAT5 on Normal Tissue Development and Cancer / Maria M. Caffarel and Christine J. Watson -- Crosstalk with the Jak-STAT Pathway in Inflammation / Lionel B. Ivashkiv -- Activation and Inhibition of JAK-STAT Signal Transduction by RNA Viruses / Curt M. Horvath -- Multifaceted Antiviral Actions of Interferon-stimulated Gene Products / Birgit Strobl, Nicole R. Leitner and Mathias Müller -- Interferon Regulation of the Innate Response to Bacteria / Ali Abdul-Sater and Christian Schindler.
  • "This volume critically examines the functional actions of the kainate type glutamate receptors (KARs). Following on from the larger body of work on the NMDA and AMPA-type ionotropic glutamate receptors (GluRs), studies with KARs have consistently thrown up exceptions to general rules about synaptic modulation. Contributors herein provide an insight to the idiosyncracies that now almost typify the KAR field. The fascinating insights provided in this volume serve to encourage searching mechanistic questions"--Provided by publisher.
  • pt. B ScienceDirect
    pt. C ScienceDirect
  • Section I. Cell protocols : basic microbiological techniques. Pouring agar plates and streaking or spreading to isolate individual colonies / Elizabeth Eyler ; Storage of bacteria and yeast / Cheryl M. Koh -- Section II. Cell protocols : cellular fractionation. Cellular fractionation : mammalian cells / Joerg von Hagen and Uwe Michelsen ; Cellular fractionation : yeast cells / Joerg von Hagen and Uwe Michelsen -- Section III. Cell protocols : Knock-outs and gene replacements. Yeast-gene replacement using PCR products / Megan Bergkessel, Christine Guthrie, and John Abelson ; Measuring RNAi knockdown using qPCR / Stuart Milstein, Michael Nguyen, Rachel Meyers, and Antonin de Fougerolles ; Recombineering : using drug cassettes to knock out genes in vivo / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court ; Gene knockouts, in vivo site-directed mutagenesis and other modifications using the delitto perfetto system in Saccharomyces cerevisiae / Samantha Stuckey and Francesca Storici ; Gene targeting and site-specific recombination in mouse ES cells / Konstantinos Anastassiadis, Frank Schnütgen, Harald von Melchner, and A. Francis Stewart ; Recombineering : highly efficient in vivo genetic engineering using single-strand oligos / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court -- Section IV. Cell protocols : microbiological media. Growth media for E. coli / Juliane C. Lessard ; Saccharomyces cerevisiae growth media / Jessica S. Dymond --Section V. Cell protocols : preparation of slides for microscopy. Immunohistochemistry on freely floating fixed tissue sections / Julia Bachman ; Preparation of slides for microscopy from frozen tissue sections / Julia Bachman ; Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry / Kirstie Canene-Adams ; Preparation of cells for microscopy using cytospin / Cheryl M. Koh ; Preparation of cells for microscopy using chamber slides and coverslips / Cheryl M. Koh ; Preparation of cells for microscopy using 'cell blocks' / Cheryl M. Koh -- Section VI. Lipid and carbohydrate protocols. Enzymatic deglycosylation of glycoproteins / Min-Sung Kim and Dan Leahy -- Section VII. Lipid and carbohydrate protocol : making vesicles and micelles. Preparation of fatty acid or phospholipid vesicles by thin-film rehydration / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Preparation of fatty acid micelles / Ting F. Zhu, Itay Budin, and Jack W. Szostak -- Section VIII. Other protocols. Reverse-phase HPLC analysis and purification of small molecules / Kirstie Canene-Adams ; Thin layer chromatography / Marina Santiago and Scott Strobel ; ATP and GTP hydrolysis assays (TLC) / Vaishnavi Rajagopal and Jon R. Lorsch.
  • Computational prediction of RNA secondary structure -- Reverse-transcription PCR (RT-PCR) -- Northern blotting -- Explanatory chapter: nuclease protection assays -- In vitro transcription from plasmid or PCR-amplified DNA -- Measurement of in vivo synthesis rates -- Method for measuring mRNA decay rate in saccharomyces cerevisiae -- Analysis of polysomes from bacteria -- Polysome profile analysis - yeast -- Polysome analysis of mammalian cells -- Polysome analysis for determining mRNA and ribosome association in saccharomyces cerevisiae -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates -- Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3' ends, and poly(A) tails of RNA -- RNA radiolabeling -- Fluorescently labeling synthetic RNAs -- Analysis of RNA by analytical polyacrylamide gel -- RNA purification by preparative polyacrylamide gel -- Explanatory chapter: nucleic acid concentration determination -- RNA purification-precipitation methods -- Reverse transcriptase dideoxy sequencing of RNA -- RNA structure experimental analysis-chemical modification -- Structural analysis of RNA backbone using in-line probing.
  • "Lactoferrin was first identified as an iron-binding glycoprotein abundantly found in milk. It has been considered to play important roles in host defense, since lactoferrin shows broad spectrum of anti-bacterial and anti-viral activities, arising from its iron-chelating property. Furthermore, lactoferrin can regulate the function of innate and adaptive immune cells and exhibits immuno-modulating properties. These activities of lactoferrin implicate that potential use of lactoferrin as a wound healing agent. In addition, this book shows that lactoferrin is a potent regulator of dermal fibroblasts and epidermal keratinocytes, and promotes skin or corneal epithelial wound healing by increasing their proliferation, migration or deposition of extracellular matrix (ECM) components such as collagen and hyaluronan. This book provides the scientific background on use of lactoferrin as a wound healing agent. Indeed, lactoferrin exerts its biological effects by binding to specific lactoferrin receptors on target cells. This book also covers the in-depth information on roles of lactoferrin as a signaling molecule."--Publisher's website.
  • Structure and synthesis of lipid A / Shoichi Kusumoto, Masahito Hashimoto and Kazuyoshi Kawahara -- Conformation and supramolecular structure of lipid A / Klaus Brandenburg and Ulrich Seydel -- Interactions between lipid A and serum proteins / Jèorg Andrèa ... [et al.] -- The lipid A receptor / Kiyoshi Takeda -- Lipid A receptor TLR4-mediated signaling pathways / Masahiro Yamamoto and Shizuo Akira -- Lipid A-induced responses in vivo / Néjia Sassi ... [et al.] -- Lipid A-mediated tolerance and cancer therapy / Cheryl E. Rockwell, David C. Morrison and Nilofer Qureshi -- Lipid A in cancer therapies : preclinical results / Daniele Reisser and Jean-François Jeannin -- Monophosphoryl lipid A (MPL) as an adjuvant for anti-cancer vaccines : clinical results / Christopher W. Cluff -- Antitumoral effects of lipids A : clinical studies / Marc Bardou and Danièle Reisser.
  • An overview of modern mass spectrometry methods in the toolbox of lipid chemists and biochemists / R. Moreau -- Techniques and applications in lipid analysis / Nils Hinrichsen and Hans Steinhart -- Recent advances in silver-ion HPLC utilizing acetonitrile in hexane as solvent: temperature effects on lipid elution orders/resolution / R.O. Adlof -- TLC-FID with special reference to marine lipids and other high-molecular-weight organic compounds / R.G. Ackman and A. Timmins -- Analysis of trans-18 :1 fatty acids by silver ion HPLC / P. Delmonte and M.P. Yurawecz -- Lipid separations using packed-column SFC / D.G. Hayes -- LC-MS and chiral separation / A. Kuksis -- LC-MS and lipid oxidation / A. Kuksis -- Structural analysis of unsaturated fatty acid methyl ester isomers with acetonitrile covalent adduct chemical ionization (CACI) / J.T. Brenna -- Fast GC for cellular fame analysis of bacteria / J.S. Buyer -- Use of cellular fatty acids to identify food-borne pathogens by infrared spectroscopy and capillary GC / M.M. Mossoba -- Infrared spectroscopy and partial least square calibration in the simultaneous quantification of isolated trans and conjugated linoleic acids / Alfred A. Christy -- Global cellular lipidome analyses by shotgun lipidomics using multidimensional mass spectrometry / X. Han and R.W. Gross -- Waxes and sterols: structures and chemistry / E.J. Parish and A.D. Bell -- Analysis of biological tissue and edible fat / J.K.G. Kramer -- Investigation of protein-lipid interactions by vibrational spectroscopy / E.C.Y. Li-Chan, G. Meng and N.K. Howell -- Fat replacers: an overview / W.E. Artz -- High-performance size-exclusion chromatography for lipid analysis in organic media / M.C. Dobarganes and G. Marquez-Ruiz -- Phospholipids : structures and physicochemical activities / M.C. Erickson.
  • This volume focusses on oxidative modifications of lipid molecules and the successive generation of singlet oxygen. The book also covers the secondary adductions of these reactive species with proteins and aminophospholipids. During lipid peroxidation, the initial event is the formation of lipid hydroperoxide, which is followed by an oxidation event that starts a chain-reaction. The formed lipid hydroperoxide gradually decomposes into harmful aldehydes, which are the advanced end-products of lipid peroxidation.
  • "Lipid Mediators and Their Metabolism in the Brain presents readers with cutting edge and comprehensive information not only on the synthesis and degradation of glycerophospholipid-, sphingolipid-, and cholesterol-derived lipid mediators, but also their involvement in neurological disorders. It is hoped that this monograph will be useful not only to postgraduate student and their teachers, but also to research scientists and physicians, who are curious about the generation and roles of lipid mediators in the brain."--Publisher's website.
  • Lipid rafts 2007, Springer Protocols
    Overview of membrane rafts / Thomas J. McIntosh -- Analysis of raft affinity of membrane proteins by detergent-insolubility / Deborah A. Brown -- Nondetergent isolation of rafts / Mehul B. Shah and Pravin B. Sehgal -- Detecting ordered domain formation (lipid rafts) in model membranes using tempo / Omar Bakht and Erwin London -- Using monomolecular films to characterize lipid lateral interactions / Rhoderick E. Brown and Howard L. Brockman -- Electro-formation and fluorescence microscopy of giant vesicles with coexisting liquid phases / Sarah L. Veatch -- Fluorescence correlation spectroscopy / Kristen Bacia and Petra Schwille -- Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells / Avery L. McIntosh ... [et al.] -- NMR detection of lipid domains / Ivan V. Polozov and Klaus Gawrisch -- Lateral diffusion coefficients of raft lipids from pulsed field gradient NMR / Greger Orädd and Göran Lindblom -- Saturation-recovery electron paramagnetic resonance discrimination by oxygen transport (DOT) method for characterizing membrane domains / Witold K. Subczynski ... [et al.] -- Plasmon-waveguide resonance spectoscopy studies of lateral segregation in solid-supported proteolipid bilayers / Zdzislaw Salamon, Savitha Devanathan, and Gordon Tollin -- Fluorescence recovery after photobleaching studies of lipid rafts / Anne K. Kenworthy -- Single-molecule tracking / Marija Vrljic, Stefanie Y. Nishimura, and W.E. Moerner -- X-ray diffraction to determine the thickness of raft and nonraft bilayers / Thomas J. McIntosh -- Small-angle neutron scattering to detect rafts and lipid domains / Jeremy Pencer ... [et al.] -- Exploring membrane domains using native membrane sheets and transmission electron microscopy / Bridget S. Wilson ... [et al.] -- Atomic force microscopy of lipid domains in supported model membranes / Alan R. Burns -- Atomistic and coarse-grained computer simulations of raft-like lipid mixtures / Sagar A. Pandit and H. Larry Scott -- A microscopic model calculation of the phase diagram of ternary mixtures of cholesterol and saturated and unsaturated phospholipids / R. Elliott, I. Szleifer, and M. Schick.
  • Lipid signaling protocols 2009, Springer Protocols
    Eicosanoids : generation and detection in mammalian cells / Valerie B. O'Donnell, Ben Maskrey, and Graham W. Taylor -- Lipidomic analysis of phospholipids and related structures by liquid chromatography-mass spectrometry / Trevor R. Pettitt -- Measurement of polyphosphoinositides in cultured mammalian cells / Frank T. Cooke -- Inositol lipid-dependent functions in Saccharomyces cerevisiae : analysis of phosphatidylinositol phosphates / Stephen K. Dove and Robert H. Michell -- Methods for the determination of the masss of nuclear PtdIns4P, PtdIns5P, and PtdIns(4,5)P₂ / David R. Jones ... [et al.] -- Lipid quantification and structure determination of nuclear envelope precursor membranes in the sea urchin / Marie Garnier-Lhomme ... [et al.] -- Solution and solid-state NMR of lipids / Axelle Grelard ... [et al.] -- Spatial localization of PtdInsP₂ in phase-separated giant unilamellar vesicles with a fluorescent PLC-delta 1 PH domain / Xavier Mulet ... [et al.] -- Studying cell-to-cell interactions : an easy method of tethering ligands on artificial membranes / Sebastian J. Fleire and Facundo D. Batista -- Ceramide-induced transbilayer (flip-flop) lipid movement in membranes / F.-Xabier Contreras ... [et al.] -- Effects of sterols on the development and aging of Caenorhabditis elegans / Eun-Young Lee ... [et al.] -- Proteomic analysis of the sterol-mediated signaling pathway in Caenorhabditis elegans / Byung-Kwon Choi ... [et al.] -- Sudying the subcellular localization and DNA-binding activity of FoxO transcription factors, downstream effectors of P13K/Akt / Abdelkader Essafi ... [et al.] -- Measurement of PTEN activity in vivo by imaging phosphorylated Akt / Erika Rosivatz and Rudiger Woscholski -- Analysis of phosphatidylinositol 3,4,5 trisphosphate 5-phosphatase activity by in vitro and in vivo assays / Lisa M. Ooms ... [et al.] -- Measuring phospholipase D activity in insulin-secreting pancreatic [beta]-cells and insulin-responsive muscle cells and adipocytes / Rosanna Cazzolli ... [et al.] -- Protein kinase C as an effector of lipid-derived second messengers / Marie-Hélène Paclet ... [et al.] -- Detection of myotubularin phosphatases activity on phosphoinositides in vitro and ex vivo / Holger Maria Rohde ... [et al.] -- Quantification of multiple phosphatidylinositol 4-kinase isozyme activities in cell extracts / Mark G. Waugh, Shane Minogue, and J. Justin Hsuan -- Phospholipid-interacting proteins by solution-state NMR spectroscopy / Keiichiro Kami, Sundaresan Rajesh, and Michael Overduin -- Revealing signaling in single cells by single- and two-photon fluorescence lifetime imaging microscopy / Damien Alcor, Veronique Calleja, and Banafshé Larijani -- Phosphoinositide (PI) 3-kinase assays / Michael J. Fry -- Measurement of phosphatidylinositol and phosphatidylcholine binding and transfer activity of the lipid transport Protein PITP / Shamshad Cockcroft -- In vitro reconstitution of activation of PLC[epsilon] by Ras and Rho GTPases / Natalia Lamuño Gandarillas ... [et al.] -- Assaying endogenous phosphatidylinositol-4-phosphate 5-kinase (PIP5K) activities / Jonathon R. Halstead ... [et al.] -- Preparation of membrane rafts / Mark G. Waugh and J. Justin Hsuan.
  • Sphingomyelin and its role in cellular signaling / Mahua Chakraborty and Xian-Cheng Jiang -- The driving force of alpha-synuclein insertion and amyloid channel formation in the plasma membrane of neural cells: key role of ganglioside- and cholesterol-binding domains / Jacques Fantini and Nouara Yahi -- The enigmatic role of sulfatides: new insights into cellular functions and mechanisms of protein recognition / Shuyan Xiao, Carla V. Finkielstein, and Daniel G. S. Capelluto -- Phosphoinositides and PDZ domain scaffolds / Anna Maria Wawrzyniak, Rudra Kashyap and Pascale Zimmermann -- PtdIns(4)P signalling and recognition systems / Marc Lenoir and Michael Overduin -- PtdIns(4,5)P2-mediated cell signaling: emerging principles and PTEN as a paradigm for regulatory mechanism / Arne Gericke, Nicholas R. Leslie, Mathias Lösche, and Alonzo H. Ross -- Role of phosphatidylinositol 3,4,5-trisphosphate in cell signaling / Robert D Riehle, Sinziana Cornea and Alexei Degterev -- The role of phosphoinositides and inositol phosphates in plant cell signaling / Glenda E. Gillaspy -- Phosphatidic acid-mediated signaling / Yu Liu, Yuan Su, and Xuemin Wang -- Phosphatidylserine-mediated cellular signaling / Jason G. Kay and Sergio Grinstein -- Cardiolipin-mediated cellular signaling / Vinay A. Patil and Miriam L. Greenberg.
  • Lipid-mediated signaling 2010, CRCnetBASE
    Methods for measuring the activity and expression of phospholipases A2 / by Prerna Rastogi, Janhavi Sharma, and Jane McHowat -- Methods for measuring the activity and expression of phospholipase C / by Ryan A. Frieler, John A. Watt, and Thad A. Rosenberger -- Methods for measuring the activity and expression of phospholipase D / by Wenjuan Su and Michael A. Frohman -- Methods for measuring the activity and expression of diacylglycerol kinase / by Kaoru Goto ... [et al.] -- Methods for measuring endocannabinoid production and expression and activity of enzymes involved in the endocannabinoid system / by Tiziana Bisogno, Luciano De Petrocellis, and Vincenzo Di Marzo -- Mass spectrometry methods for the analysis of lipid molecular species: a shotgun lipidomics approach / by Xianlin Han ... [et al.] -- Mass spectrometry methods for the analysis of bioactive sphingolipids : a high-performance liquid-chromatography/tandem mass spectrometry approach / by Jacek Bielawski, Motohiro Tani, and Yusuf A. Hannun -- Mass spectrometry methods for the analysis of oxidant stress in biological fluids and tissues : quantification of F2-isoprostanes and F4-neuroprostanes using mass spectrometry / by Ginger L. Mine and Jason D. Morrow -- Methods for assessing the role of lipid-activated nuclear receptors in regulation of gene transcription / by Jenny Kaeding, Jocelyn Trottier, and Olivier Barbier -- Fluorescence methods to assess the impact of lipid binding proteins on ligand-mediated activation of gene expression / by Heather A. Hostetler ... [et al.] -- Methods for measuring fatty acids using chromatographic techniques / by Gwendolyn Barecló-Coblijn, Cameron C. Murphy, and Eric J. Murphy -- Methods for measuring fatty acid uptake and targeting in cultured cells and in vivo / by Paul G. Millner, Serena M. Lackman, and Eric J. Murphy -- Methods for kinetic analysis of fatty acid incorporation and turnover in vivo : a steady-state kinetic radiotracer approach / by Dhaval P. Bhatt and Thad A. Rosenberger.
  • Offers an account of the properties of the classes of lipids that are located in the membranes of various cells. This title presents a balanced perspective of what is known about the function of these lipids in healthy cells and tissues and what consequences follow malfunctions in their synthesis and metabolism.
  • Lipids. 1st ed. 2012, ScienceDirect
    Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding / Min Wu, Pietro de Camilli -- Studying lipids involved in the endosomal pathway / Christin Bissig, Shem Johnson, Jean Gruenberg -- Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking / Jean-Baptiste Manneville, Cécile Leduc, Benoit Sorre, Guillaume Drin -- Reconstituting multivesicular body biogenesis with purified components / Thomas Wollert -- Approaches to the study of Atg8-mediated membrane dynamics in vitro / Anjai Jotwani, Diana N. Richerson, Isabelle Motta, Omar Julca-Zevallos, Thomas J. Melia -- Reconstitution assay system for ceramide transport with semi-intact cells / Keigo Kumagai, Masahiro Nishijima, Kentaro Hanada -- Visualizing mitochondrial lipids and fusion events in mammalian cells / Huiyan Huang, Michael A. Frohman -- Targeted and non-targeted analysis of membrane lipids using mass spectrometry / Xue Li Guan, Markus R. Wenk -- Modulation of host phosphoinositide metabolism during salmonella invasion by the type III secreted effector SopB / Bernhard Roppenser, Sergio Grinstein, John H. Brumell -- Acute manipulation of phosphoinositide levels in cells / Belle Chang-Ileto, Samual G. Frere, Gilbert Di Paolo -- Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins / Marnix Wieffer, Volker Haucke, Michael Krauß -- Phosphoinositides at the neuromuscular junction of drosophila melanogaster : a genetic approach / Jan R. Slabbaert, Thang Manh Khuong, Patrik Verstreken -- Devising powerful genetics, biochemical and structural tools in the functional analysis of phosphatidylinositol transfer proteins (PITPs) across diverse species / James M. Davison, Vytas A. Bankaitis, Ratna Ghosh -- Genome-wide screens for gene products regulating lipid droplet dynamics / Weihua Fei, Hongyuan Yang -- The three dimensionality of cell membranes : lamellar to cubic membrane transition as investigated by electron microsocopy / Ketpin Chong, Yuru Deng -- Quantitative imaging of lipid metabolism in yeast : from 4D analysis to high content screens of mutant libraries / Heimo Wolinski, Kristian Bredies, Sepp D. Kohlwein -- Analysis of cholesterol trafficking with fluorescent probes / Frederick R. Maxfield, Daniel Wüstner -- Fluorescence correlation methods for imaging cellular behavior of sphingolipid-interacting probes / Rachel Kraut, Nirmalya Bag, Thorsten Wohland -- Monitoring phospholipid dynamics during phagocytosis : application of genetically-encoded fluorescent probes / Helen Sarantis, Sergio Grinstein -- Genetically encoded probes for phosopatidic acid / Nawal Kassas, Petra Tryoen-Tóth, Matthias Corrotte, Tamou Thahouly, Marie-France Bader, Nancy J. Grant, Nicolas Vitale.
  • Liposomes. Part A-G pt. A-G, 2003-09, ScienceDirect
    pt. B, 2003 ScienceDirect
    pt. C, 2003 ScienceDirect
    pt. D, 2004 ScienceDirect
    pt. E, 2005 ScienceDirect
    pt. F, 2009 ScienceDirect
    pt. G, 2009 ScienceDirect
  • ch. 1 Introduction, p. 1-5 -- ch. 2 Liquid Chromatography, p. 7-31 -- ch. 3 Mass Spectrometry, p. 33-74 -- ch. 4 Interface Technology, p. 75-127 -- ch. 5 Applications of High Performance Liquid Chromatography-Mass Spectrometry, p. 129-234.
  • Protein kinase C-theta (PKC[theta]): new perspectives on its functions in T-cell biology / Rina Barouch-Bentov and Amnon Altman -- Travel informations on the tec kinases during lymphocyte activation / Fabien Garçon and Jacques A. Nunès -- Inducible T-cell tyrosine kinase (ITK): structural requirements and actin polymerization / Constantine D. Tsoukas ... [et al.] -- Differential requirements of PI3K subunits for BCR or BCR/CD19-induced ERK activation / Elena Vigorito and Martin Turner -- Protein tyrosine phosphatases in human disease / Tomas Mustelin -- The T-cell-specific adapter protein functions as a regulator of peripheral but not central immunological tolerance / Philip E. Lapinski ... [et al.] -- Proximal signals controlling B-cell antigen receptor (BCR) mediated NF-[kappa]B activation / Miguel E. Moreno-García ... [et al.] -- The adapter 3BP2: how it plugs into leukocyte signaling / Marcel Deckert and Robert Rottapel -- CTLA-4 regulation of T-cell function via RAP-1-mediated adhesion / Helga Schneider ... [et al.] -- Protein crosstalk in lipid rafts / Raquel J. Nunes, Mónica A.A. Castro, and Alexandre M. Carmo -- Role of lipid rafts in activation-induced cell death: the fas pathway in aging / Anis Larbi ... [et al.] -- T-cell response in aging: influence of cellular cholesterol modulation / Tamàs Fülöp ... [et al.] -- Lymphocyte signaling and the translatability of mRNA / Suzanne Miyamoto -- Protein arginine methylation: a new frontier in T-cell signal transduction / Brandon T. Schurter, Fabien Blanchet, and Oreste Acuto -- Immune regulation by ubiquitin conjugation / K. Venuprasad ... [et al.] -- CTIB (C-terminus protein of I[kappa]B-[beta]): a novel factor required for acidic adaptation /Toshiko Fukamachi ... [et al.] -- HIV may deplete most CD4 lymphocytes by a mechanism involving signaling through its receptors on non-permissive resting lymphocytes / Miles W. Cloyd ... [et al.] -- Integrating traditional and postgenomic approaches to investigate lymphocyte development and function / Yina Hsing Huang ... [et al.] -- Phosphoproteomic analysis of lymphocyte signaling / Lulu Cao, Kebing Yu, and Arthur R. Salomon.
  • Introduction: brief historical overview -- Distribution of MACPF/CDC proteins -- Evolution of the complement system -- Structural features of cholesterol dependent cytolysins and comparison to other MACPF-domain containing proteins -- Perfringolysin O structure and mechanism of pore formation as a paradigm for cholesterol-dependent cytolysins -- Structural biology of the membrane attack complex -- Membrane interactions and cellular effects of MACPF/CDC proteins -- The biology of pneumolysin -- Multifaceted activity of listeriolysin O, the cholesterol -dependent cytolysin of Listeria monocytogenes -- Perforin: a key pore-forming protein for immune control of viruses and cancer -- Perforin and human diseases -- The role of MACPF proteins in the biology of malaria and other apicomplexan parasites -- Chlamydial MACPF protien CT153 -- Fungal MACPF-like proteins and aegerolysins: bi-component pore-forming proteins? -- Fluorescence imaging of MACPF/CDC proteins: new techniques and their application.
  • MAP kinase signaling protocols. 2nd ed. 2010, Springer Protocols
    The MAP kinase signaling cascades : A system of hundreds of components regulates a diverse array of physiological functions -- Determination of ERK activity: Anti-phospho-ERK antibodies and in vitro phosphorylation -- Activation of SAPK/JNKs in vitro -- Activation of p38 and determination of its activity -- Activity assays for extracellular signal-regulated kinase 5 -- Use of inhibitors in the study of MAP kinases -- MAP kinase activation by receptor tyrosine kinases : in control of cell migration -- Activation of ras and rho GTPases and MAP kinases by G-protein-coupled receptors -- Regulation of MAP kinase signaling by calcium -- Identification of novel substrates of MAP kinase cascades using bioengineered kinases that uniquely utilize analogs of ATP to phosphorylate substrates -- ERK-MAP kinase signaling in the cytoplasm -- Lentiviral vectors to study the differential function of ERK1 and ERK2 MAP kinases -- Structural studies of MAP kinase cascade components -- Analysis of MAP kinases by hydrogen exchange mass spectrometry -- A "Molecular evolution" approach for isolation of intrinsically active (MEK-independent) MAP kinases -- Reconstitution of the nuclear transport of the MAP kinase ERK2 -- Localization and trafficking of fluorescently tagged ERK1 and ERK2 -- Studying the regulation of MAP kinase by MAP kinase phosphatases in vitro and in cell systems -- Proteomic analysis of scaffold proteins in the ERK cascade -- Analysis of ERKs' dimerization by electrophoresis -- MAP kinase: SUMO pathway interactions -- Computational modelling of kinase signalling cascades -- Analysis of mitogen-activated protein kinase activity in yeast -- Detection of RTK pathway activation in drosophila using anti-dpERK immunofluorescence staining -- Studying MAP kinase pathways during early development of xenopus laevis -- Deciphering signaling pathways in vivo : The ras/raf/MEK/ERK cascade -- Mutational and functional analysis in human ras/MAP kinase genetic syndromes -- Implication of the ERK pathway on the post-transcriptional regulation of VEGF mRNA stability -- Studies on MAP kinase signaling in the immune system -- Methods to study MAP kinase signalling in the central nervous system -- MAP kinase regulation of the mitotic spindle checkpoint -- Using high-content microscopy to study gonadotrophin-releasing hormone regulation of ERK.
  • Generation and analysis of multidimensional protein identification technology datasets / Selene K. Swanson, Laurence Florens, and Michael P. Washburn -- Quantitative peptide and protein profiling by mass spectrometry / Alexander Schmidt, Birgit Bisle, and Thomas Kislinger -- Label-free relative quantitation of prokaryotic proteomes using the accurate mass and time tag approach / Kim K. Hixson -- Classical proteomics: two-dimensional electrophoresis/MALDI mass spectrometry / Ursula Zimny-Arndt ... [et al.] -- The use of difference in-gel electrophoresis for quantitation of protein expression / Rajat Sapra -- Liquid-chromatography-mass spectrometry of thylakoid membrane proteins / Christian G. Huber ... [et al.] -- High accuracy mass spectrometry in large-scale analysis of protein phosphorylation / Jesper V. Olsen and Boris Macek -- Manual validation of peptide sequence and sites of tyrosine phosphorylation from MS/MS spectra / Amy M. Nichols and Forest M. White -- Assigning glycosylation sites and microheterogeneities in glycoproteins by liquid chromatography/tandem mass spectrometry / Yehia Mechref, Milan Madera, and Milos V. Novotny -- Structure analysis of N -glycoproteins / Stefanie Henning, Jasna Peter-Katalinicʹ, and Gottfried Pohlentz -- Capillary zone electrophoresis-mass spectrometry for the characterization of isoforms of intact glycoproteins / Christian Neusüss, and Matthias Pelzing -- Top-down proteomics on a high-field fourier transform ion cyclotron resonance mass spectrometer / Séverine A. Ouvry-Patat ... [et al.] -- Capillary isoelectric focusing/reversed phase liquid chromatography/mass spectrometry / Cheng S. Lee and Brian M. Balgley -- Integrating accelerated tryptic digestion into proteomics workflows / Gordon W. Slysz and David C. Schriemer -- Hydrogen/deuterium exchange mass spectrometry / Xuguang Yan and Claudia S. Maier -- Mass spectrometry detection and characterization of noncovalent protein complexes / Sheng Yin and Joseph A. Loos -- Chemical cross-linking for protein-protein interaction studies / Xiaoting Tang and James E. Bruce -- Tissue analysis with high-resolution imaging mass spectrometry / A.F. Maarten Altelaar and Ron M.A. Heeren -- Proteomic global profiling for cancer biomarker discovery / Vitor Faca, Hong Wang, and Samir Hanash -- Analysis of protein glycosylation and phosphorylation using liquid phase separation, protein microarray technology, and mass spectrometry / Jia Zhao ... [et al.] -- Transthyretin mass determination for detection of transthyretin familial amyloid / John F. O'Brien and H. Robert Bergen III -- Characterization of microorganisms by MALDI mass spectrometry / Catherine E. Petersen, Nancy B. Valentine, and Karen L. Wahl -- Mass spectrometric characterization of neuropeptides / Stephanie S. DeKeyser, James A. Dowell, and Lingjun Li -- Peptide and protein ion/ion reactions in electrodynamic ion traps: tools and methods / Scott A. McLuckey -- Electron capture dissociation LC/MS/MS for bottom-up proteomics / Roman A. Zubarev -- Two-dimensional ion mobility analyses of proteins and peptides / Alexandre A. Shvartsburg, Keqi Tang, and Richard D. Smith -- Proteomics for validation of automated gene model predictions / Kemin Zhou ... [et al.] -- Support vector machines for improved peptide identification from tandem mass spectrometry database search / Bobbie-Jo M. Webb-Robertson.
  • Matrix Metalloproteinases / Viola Vargová, Marek Pytliak and Viola Mechírová -- Specificity of Binding with Matrix Metalloproteinases / Satya P. Gupta and Vaishali M. Patil -- The Gelatinases and Their Inhibitors: The Structure-Activity Relationships / Snehasikta Swarnakar, Amartya Mishra and Susri Ray Chaudhuri (Guha) -- Advances in Studies on Collagenase Inhibitors / Mange Ram Yadav, Prashant Revan Murumkar and Vishal Prakash Zambre -- Hydroxamic Acids as Matrix Metalloproteinase Inhibitors / Rajeshwar P. Verma -- Quantitative Structure-Activity Relationship Studies on Sulfonamide-Based MMP Inhibitors / Vaishali M. Patil and Satya P. Gupta -- Matrix Metalloproteinase Inhibitors as Investigative Tools in the Pathogenesis and Management of Vascular Disease / Mina M. Benjamin and Raouf A. Khalil.
  • This textbook aims to describe the fascinating area of eukaryotic gene regulation for graduate students in all areas of the biomedical sciences. Gene expression is essential in shaping the various phenotypes of cells and tissues and as such, regulation of expression is a fundamental aspect of nearly all processes in physiology, both in healthy and in diseased states. This pivotal role for the regulation of gene expression makes this textbook essential reading from students of all the biomedical sciences in order to be better prepared for their specialized disciplines. A complete understanding of transcription factors and the processes that alter their activity is a major goal of modern life science research. The availability of the whole human genome sequence (and that of other eukaryotic genomes) and the consequent development of next-generation sequencing technologies have significantly changed nearly all areas of the biological sciences. For example, the genome-wide location of histone modifications and transcription factor biding sites, such as provided by the ENCODE consortium, has greatly improved our understanding of gene regulation. Therefore, the focus of this book is the description of the post-genome understanding of gene regulation. The purpose of this book is to provide, in a condensed form, an overview on the present understanding of the mechanisms of gene regulation.
  • Medical biochemistry. 4th ed. 2002, ScienceDirect
  • Brought to you in a thorough yet accessible manner, the new edition of Medical Biochemistry gives access to all of the latest information on basic and clinically focused genetic and molecular biology. Featuring a team of contributors that includes investigators involved in cutting-edge research as well as experienced clinicians, this updated medical textbook offers a unique combination of both research and practice that's ideal for today's problem-based integrated courses. Relate biochemistry to everyday practice with the help of Clinical Boxes integrated into the text, and access in-depth coverage of important topics - including recent research in biochemistry - through Advanced Concept Boxes.Test your knowledge and improve retention with Active Learning Boxes at the conclusion of each chapter, and quickly review the most common lab tests performed with convenient Clinical Test Boxes.Effectively study the most updated information in biochemistry with the help of a dynamic, full-color design.Better understand the relationship between science and clinical practice with material organized by organ rather than system.
  • Structure-activity relationships (SAR) of melanocortin and agouti-related (AGRP) peptides / Anamika Singh, Erica M. Haslach and Carrie Haskell-Luevano -- Melanocortin signalling mechanisms / Paula C. Eves and John W. Haycock -- Distribution and function of melanocortin receptors within the brain / Kathleen G. Mountjoy -- Drugs, exercise, and the melanocortin-4 receptor: different means, same ends: treating obesity / Jay W. Schaub, Erin B. Bruce, and Carrie Haskell-Luevano -- Melanocortins in brain inflammation: the role of melanocortin receptor subtypes / Ruta Muceniece and Maija Dambrova -- Melanocortins and the cholinergic anti-inflammatory pathway / Daniela Giuliani ... [et al.] -- Melanocortin control of cell trafficking in vascular inflammation / Hetal B. Patel ... [et al.] -- Terminal signal: anti-inflammatory effects of α-melanocyte-stimulating hormone related peptides beyond the pharmacophore / Thomas Brzoska ... [et al.] -- Protective effects of melanocortins in systemic host reactions / Stefano Gatti ... [et al.] -- Development of α-melanocortin analogs for melanoma prevention and targeting / Zalfa A. Abdel-Malek -- MSH radiopeptides for targeting melanoma metastases / Alex N. Eberle ... [et al.] -- Applications of the role of α-MSH in ocular immune privilege / Andrew W. Taylor and Darren Lee.
  • The role of ion channels in hypoxic pulmonary vasoconstriction / E. Kenneth Weir ... [et al.] -- Two-pore domain K channels and their role in chemoreception / Keith J. Buckler -- Intricate interaction between store-operated calcium entry and calcium-activated chloride channels in pulmonary artery smooth muscle cells / Abigail S. Forrest ... [et al.] -- The role of intracellular ion channels in regulating cytoplasmic calciumin pulmonary arterial mmooth [i.e. smooth] muscle : which store and where? / A. Mark Evans -- Ca2 oscillations regulate contraction of intrapulmonary smooth muscle cells / Michael J. Sanderson, Yan Bai, and Jose Perez-Zoghbi -- Introduction to TRP channels : structure, function, and regulation / Michael Y. Song and Jason S.-J. Yuan -- Physiological functions of transient receptor potential channels in pulmonary arterial smooth muscle cells / Xiao-Ru Yang, Mo-Jun Lin, and James S.K. Sham -- The contribution of TRPC1 and STIM1 to capacitative Ca2 entry in pulmonary artery / Lih Chyuan Ng, Judith A. Airey, and Joseph R. Hume -- Store-operated calcium entry channels in pulmonary endothelium : the emerging story of TRPCS and Orai1 / Donna L. Cioffi, Christina Barry, and Troy Stevens -- TRPM2 channel regulates endothelial barrier function / Claudie M. Hecquet, Gias U. Ahmmed, and Asrar B. Malik -- A proposed mitochondrial-metabolic mechanism for initiation and maintenance of pulmonary arterial hypertension in fawn-hooded rats : the Warburg model of pulmonary arterial hypertension / Jalees Rehman and Stephen L. Archer -- The role of classical transient receptor potential channels in the regulation of hypoxic pulmonary vasoconstriction / B. Fuchs ... [et al.] -- Developmental regulation of oxygen sensing and ion channels in the pulmonary vasculature / David N. Cornfield -- Hypoxic regulation of ion channels and transporters in pulmonary vascular smooth muscle / Larissa A. Shimoda -- CLC-3 chloride channels in the pulmonary vasculature / Joseph R. Hume ... [et al.] -- Role of bone morphogenetic protein receptors in the development of pulmonary arterial hypertension / Nicholas W. Morrell -- Cross talk between Smad, MAPK, and actin in the etiology of pulmonary arterial hypertension / James West -- Notch signaling in pulmonary hypertension / Patricia A. Thistlethwaite, Xiaodong Li, and Xiaoxue Zhang -- Rho kinase-mediated vasoconstriction in pulmonary hypertension / Ivan F. McMurtry ... [et al.] -- The serotonin hypothesis of pulmonary hypertension revisited / Margaret R. MacLean and Yvonne Dempsie -- Impaired vascular endothelial growth factor signaling in the pathogenesis of neonatal pulmonary vascular disease / Steven H. Abman -- Mitochondrial regulation of oxygen sensing / Navdeep S. Chandel -- Reactive oxygen species and RhoA signaling in vascular smooth muscle : role in chronic hypoxia-induced pulmonary hypertension / Thomas C. Resta, Brad R.S. Broughton, and Nikki L. Jernigan -- Polyamine regulatory pathways as pharmacologic targets in pulmonary arterial hypertension / Mark N. Gillespie and Jack W. Olson -- 5-HT receptors and Kv channel internalization / Angel Cogolludo and Francisco Perez-Vizcaino -- KCNQ potassium channels : new targets for pulmonary vasodilator drugs? / Alison M. Gurney, Shreena Joshi, and Boris Manoury -- Receptor tyrosine kinase inhibitors in rodent pulmonary hypertension / Liliana Moreno-Vinasco and Joe G.N. Garcia -- PDGF receptor and its antagonists : role in treatment of PAH / Friedrich Grimminger and Ralph Theo Schermuly -- PPAR[gamma] and the pathobiology of pulmonary arterial hypertension / Marlene Rabinovitch -- Targeting TASK-1 channels as a therapeutic approach / Andrea Olschewski -- Pharmacological targets for pulmonary vascular disease : vasodilation versus anti-remodelling / Matthew Thomas.
  • Acid amides -- Amidines, guanidines, and hydrazines -- Amino acids: phosphinic acids -- Anilines and nitrobenzenes -- Aryloxy acids -- Biphenyl ethers -- Carbamates -- Dithio- and thiolcarbamates -- Halogenated aliphatics -- Halogenated aromatics -- Five-membered heterocycles -- Five-membered heterocycles (fused) -- Six-/more membered heterocycles -- Six-/more membered heterocycles (fused) -- Imides -- Natural products -- Organophosphorous compounds -- Oximes -- Phenylureas and related compounds -- Phosphono amino acids and related compounds -- Pyrethroids -- Sulfonylureas -- Triazines and related compounds -- Miscellaneous.
  • Origins of metabolic profiling / Arthur B. Robinson and Noah E. Robinson -- Amino acid profiling for the diagnosis of inborn errors of metabolism / Monique Piraud ... [et al.] -- Acylcarnitines : analysis in plasma and whole blood using tandem mass spectrometry / David S. Millington and Robert D. Stevens -- Analysis of organic acids and acylglycines for the diagnosis of related inborn errors or metabolism by GC- and HPLC-MS / Giancarlo la Marca and Cristiano Rizzo -- HPLC analysis for the clinical-biochemical diagnosis of inborn errors of metabolism of purines and pyrimidines / Giuseppe Lazzarino ... [et al.] -- Bile acid analysis in various biological samples using ultra performance liquid chromatography/electrospray ionization-mass spectrometry (UPLC/ESI-MS) / Masahito Hagio, Megumi Matsumoto, and Satoshi Ishizuka -- Analysis of glycolytic intermediates with ion chromatography- and gas chromatography-mass spectrometry / Jan C. van Dam, Cor Ras, and Angela ten Pierick -- Analysis of the citric acid cycle intermediates using gas chromatography-mass spectrometry / Rajan S. Kombu, Henri Brunengraber, and Michelle A. Puchowicz -- Quantification of pentose phosphate pathway (PPP) metabolites by liquid chromatography-mass spectrometry (LC-MS) / Amber Jannasch, Miroslav Sedlak, and Jiri Adamec -- High-performance liquid chromatography-mass spectrometry (HPLC-MS)-based drug metabolite profiling / Ian D. Wilson -- Gas chromatography-mass spectrometry (GC-MS)-based metabolomics / Antonia Garcia and Coral Barbas -- Use of two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOF-MS) for metabolomic analysis of polar metabolites / Kimberly Ralston-Hooper ... [et al.] -- LC-MS-based metabolomics / Sunil Bajad and Vladimir Shulaev -- Capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS)-based metabolomics / Philip Britz-McKibbin -- Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics for studies of body fluids and tissues / Heli Nygren ... [et al.] -- Electrospray ionization tandem mass spectrometry (ESI-MS/MS)- based shotgun lipidomics / Giorgis Isaac -- Processing and analysis of GC/LC-MS based metabolomics data / Elizabeth Want and Perrine Masson -- Nuclear magnetic resonance (NMR)-based drug metabolite profiling / Eva M. Lenz -- Nuclear magnetic resonance (NMR)-based metabolomics / Hector C. Keun and Toby J. Athersuch -- Slow magic angle sample spinning : a non- or minimally invasive method for high-resolution ¹H nuclear magnetic resonance (NMR) metabolic profiling / Jian Zhi Hu -- Processing and modeling of nuclear magnetic resonance (NMR) metabolic profiles / Timothy M. D. Ebbels, John C. Lindon, and Muireann Coen.
  • Metal ions in the brain are a necessity as well as a poison. The presence of metal ions in the active sites of biological catalysts or metalloproteins and in the biological functioning of nucleic acids is very well documented and they are required for brain activity. On the other hand, metals are very effective in generating oxidative stress. This effect does not only play a role in immunology but also is the root of practically all neurodegenerative disorders by inducing disease via the death of neurons. Managing metal ions in the brain could therefore be an important strategy in the search for therapeutic agents used in the treatment of neurodegenerative diseases. This new title gives an overview to key topics in the area of metal ions in the brain. It focuses on the role of metal ions in neurological systems by describing their advantageous functions as well as their poisonous features. It is therefore of interest for scientists in biochemistry and biophysics, physiology, toxicology as well as for physicians focused on this topic.
  • Methods in enzymology v. 1-, 1955-, ScienceDirect
  • Methods in membrane lipids 2007, Springer Protocols
  • 1. Techniques for cultivating methanogens / Ralph S. Wolfe -- 2. Genetic methods for methanosarcina species / Nicole Buan, Gargi Kulkarni, and William Metcalf -- 3. Genetic systems for hydrogenotrophic methanogens / Felipe Sarmiento B., John A. Leigh, and William B. Whitman -- 4. Molecular tools for investigating ANME community structure and function / Steven J. Hallam ... [et al.] -- 5. Studying gene regulation in methanogenic archaea / Michael Rother, Christian Sattler, and Tilmann Stock -- 6. Growth of methanogens under defined hydrogen conditions / John A. Leigh -- 7. Preparation of [Fe]-hydrogenase from methanogenic archaea / Seigo Shima, Michael Schick, and Haruka Tamura -- 8. Assay of methylotrophic methyltransferases from methanogenic archaea / Donald J. Ferguson, David G. Longstaff, and Joseph A. Krzycki -- 9. Methyl-coenzyme M reductase from methanothermobacter marburgensis / Evert C. Duin, Divya Prakash, and Charlene Brungess -- 10. Methods for analysis of acetyl-CoA synthase : applications to bacterial and archaeal systems / David A. Grahame -- 11. Acetate kinase and phosphotransacetylase / James G. Ferry -- 12. Sodium ion translocation and ATP synthesis in methanogens / Katharina Schlegel and Volker Müller -- 13. Proton translocation in methanogens / Cornelia Welte and Uwe Deppenmeier -- 14. Measuring isotope fractionation by autotrophic microorganisms and enzymes / Kathleen M. Scott, Gordon Fox, and Peter R. Girguis -- 15. 2-oxoacid metabolism in methanogenic CoM and CoB biosynthesis / David E. Graham -- 16. Biomethanation and its potential / Irini Angelidaki ... [et al.].
  • Methods of adipose tissue biology. Part A-B pt. A-B, 2014., ScienceDirect
    pt. B ScienceDirect
  • Microarray technology has made strong progress over the past decade, and there have also been significant changes in application areas, from nucleic acids to proteomics and from research to clinical applications. This book provides a comprehensive overview of microarrays in diagnostics and biomarker development, covering DNA, peptide, protein and tissue arrays. The focus is on entities that are in actual clinical use, or quite close, and on recent developments, such as peptide and aptamer arrays. A further topic is the miniaturisation towards nanoarrays, which is expected to have great potential in clinical applications. Relevant issues of bioinformatics and statistical analysis of array data are discussed in detail, as well as the barriers to the commercialisation of array-based tests and the vexing IP issues involved. Thus, the book should be very useful tor active array users as well as to newcomers seeking to make the best choice between different technologies.
  • This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers microbial metagenomics, metatranscriptomics, and metaproteomics, and includes chapters on such topics as in-solution FISH for single cell genome preparation, preparation of BAC libraries from marine microbial community DNA, and preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton. Continues the legacy of this premier serial with quality chapters authored by leaders in the field.
  • MicroRNA methods 2007, ScienceDirect
  • Microtubule protocols 2007, Springer Protocols
  • Aquaporins : a family of highly regulated multifunctional channels / Charles Hachez and François Chaumont -- Phylogeny of major intrinsic proteins / Jonas Å.H. Danielson and Urban Johanson -- Metalloids, soil chemistry, and the environment / Enzo Lombi and Peter E. Holm -- Arsenic transport in prokaryotes and eukaryotic microbes / Barry P. Rosen and Markus J. Tamøs -- Metalloid transport by aquaglyceroporins : consequences in the treatment of human diseases / Rita Mukhopadhyay and Eric Beitz -- Roles of vertebrate aquaglyceroporins in arsenic transport and detoxification / Zijuan Liu -- Molecular mechanisms of boron transport in plants : involvement of arabidopsis NIP5;1 and NIP6;1 / Kyoko Miwa ... [et al.] -- Silicon transporters in higher plants / Jian Feng Ma -- Major intrinsic proteins and arsenic transport in plants : new players and their potential roles / Gerd P. Bienert and Thomas P. Jahn -- Major intrinsic proteins in biomimetic membranes / Claus Hølix Nielsen.
  • Introduction to mitochondria / Carolyn D. Berdanier -- Mitochondrial bioenergetics in intact tissue / Richard L. Veech -- The human mitochondrial genome: mechanisms of expression and maintenance / Jan-Willem Taanman and Sion Llewellyn Williams -- Oxidative phosphorylation disease: diagnosis and pathogenesis / John M. Shoffner -- Using mitochondrial DNA in population surveys / Carolyn D. Berdanier -- Mitochondrial DNA mutation, oxidative stress, and alteration of gene expression in human aging / Hsin--Chen Lee and Yau-Huei Wei -- Mitochondrial DNA in cardiomyopathies / Nobuakira Takeda -- Mitochondria in Diabetes mellitus / Hong Kyu Lee -- Drugs, nutrients, and hormones in mitochondrial function / Carolyn D. Berdanier -- Bacterial FF ATP synthase as a model for complex V / Brian D. Cain, Tammy Bohannon Grabar, and Depa Bhatt -- Cybrids in the study of animal mitochondrial genetics and pathology / Ian A. Trounce and Carl A. Pinkert -- Animal modeling: from transgenesis to transmitochondrial models / Carl A. Pinkert and Ian A. Trounce -- BHE/Cdb rats as tools to study mitochondrial gene expression / Carolyn D. Berdanier and Moon-Jeong Chang Kim -- Appendix 1 -- Appendix 2.
  • Regulation of mitochondrial respiration in heart muscle / Ilmo Hassinen -- Regulation of fatty acid oxidation of the heart / Clifford D.L. Folmes and Gary D. Lopaschuk -- Regulation of mitochondrial fuel handling by the peroxisome proliferator-activated receptors / Mary C. Sugden and Mark J. Holness -- Molecular structure of the mitochondrial citrate transport protein / Ronald S. Kaplan and June A. Mayor -- Regulation of pyruvate and amino acid metabolism / Thomas C. Vary, Wiley W. Souba, and Cristopher J. Lynch -- Amino acids and the mitochondria / Nicola King -- Mechanotransduction of shear-stress at the mitochondria / Abu-Bakr Al-Mehdi -- Formation of reactive oxygen species in mitochondria / Julio F. Turrens -- Mitochondrial calcium : role in the normal and ischaemic/reperfused myocardium / Elinor J. Griffiths ... [et al.] -- Mitochondrial ion channels / Brian O'Rourke -- The mitochondrial permeability transition pore : from molecular mechanism to reperfusion injury and cadioprotection / Andrew P. Halestrap, Samantha J. Clarke, and Igor Khalilin -- The apoptotic mitochondrial pathway : modulators, interventions, and clinical implications / M.-Saadeh Suleiman and Stephen W. Schaffer -- The role of mitochondria in necrosis following myocardial ischemia-reperfusion / Elizabeth Murphy and Charles Steenbergen -- Mitochondria and their role in ischemia/reperfusion injury / Sebastian Phillip, James M. Downey, and Michael V. Cohen -- Mitochondrial DNA damage and repair / Inna N. Shokolenko, Susan P. Ledoux, and Glenn L. Wilson.
  • Mitochondrial cytopathies are mutations in the inherited maternal mitochondrial genome, or the nuclear DNA-mutation. Mitochondrial respiratory chain disorders (RCD) are a group of genetically and clinically heterogeneous diseases, due to the fact that protein components of the respiratory chain are encoded by both mitochondrial and nuclear genomes and are essential in all cells. In addition, the biogenesis, structure and function of mitochondria, including DNA replication, transcription, and translation, all require nuclear encoded genes. Since mitochondria are present in every cell, every tissue, mitochondrial disorders usually affects multiple organs. Mitochondrial Disorders Caused by Nuclear Genes discusses the biochemical, molecular, clinical, and genetic aspects of complex dual genome mitochondrial disorders. Chapters include genes involved in mitochondrial DNA biogenesis and maintenance of mitochondrial DNA integrity, complex subunits and assembly genes, and mitochondrial protein translation related diseases.
  • Mitochondrial function 2014, ScienceDirect
  • Introduction to Mitochondrial Oxidative Phosphorylation / Bernhard Kadenbach -- Mitochondrial Dynamics: The Intersection of Form and Function / Andrew Ferree and Orian Shirihai -- Biogenesis of Mitochondrial Proteins / Johannes M. Herrmann, Sebastian Longen, Daniel Weckbecker and Matthieu Depuydt -- Assembly Factors of Human Mitochondrial Respiratory Chain Complexes: Physiology and Pathophysiology / Daniele Ghezzi and Massimo Zeviani -- Supramolecular Organisation of the Mitochondrial Respiratory Chain: A New Challenge for the Mechanism and Control of Oxidative Phosphorylation / Giorgio Lenaz and Maria Luisa Genova -- Molecular Mechanisms of Superoxide Production by the Mitochondrial Respiratory Chain / Stefan Dröse and Ulrich Brandt -- Studies on the Function and Regulation of Mitochondrial Uncoupling Proteins / Richard K. Porter -- Evolution of the Couple Cytochrome c and Cytochrome c Oxidase in Primates / Denis Pierron, Derek E. Wildman, Maik Hüttemann, Thierry Letellier and Lawrence I. Grossman -- Reaction Mechanism of Mammalian Mitochondrial Cytochrome c Oxidase / Shinya Yoshikawa, Kazumasa Muramoto and Kyoko Shinzawa-Itoh -- Phosphorylation of Mammalian Cytochrome c and Cytochrome c Oxidase in the Regulation of Cell Destiny: Respiration, Apoptosis, and Human Disease / Maik Hüttemann, Icksoo Lee, Lawrence I. Grossman, Jeffrey W. Doan and Thomas H. Sanderson -- Individual Biochemical Behaviour Versus Biological Robustness: Spotlight on the Regulation of Cytochrome c Oxidase / Rabia Ramzan, Petra Weber, Bernhard Kadenbach and Sebastian Vogt -- Bigenomic Regulation of Cytochrome c Oxidase in Neurons and the Tight Coupling Between Neuronal Activity and Energy Metabolism / Margaret T. T. Wong-Riley -- Cytochrome c Oxidase and Its Role in Neurodegeneration and Neuroprotection / Susanne Arnold -- The Many Clinical Faces of Cytochrome c Oxidase Deficiency / Salvatore DiMauro, Kurenai Tanji and Eric A. Schon.
  • This practical introduction to molecular modelling of GPCRs covers core principles, and moves on to present advanced GPCR modelling techniques. Includes data on crystal structures and essential amino acid sequences, as well as LINUX commands and examples.
  • Chromatin regulation by long non-coding RNAs / Daniel C. Factor, Paul J. Tesar and Ahmad M. Khalil -- Regulatikon of eukaryotic cell differentiation by long non-coding RNAs / Juan R. Alvarez-Dominguez, Wenqian Hu and Harvey F. Lodish -- Roles of long non-coding RNAs in X-chromsome inactivation / J. Mauro Calabrese and Terry Magnuson -- Roles of long non-coding RNAs in genomic imprinting / Kristen Martins-Taylor and Stormy J. Chamberlain -- Dysregulation of long non-coding RNAs in human disease / Nianwei Lin and Tariq M. Rana -- Functions of long non-coding RNAs in non-mamallian systems / Alex Tuck and David Tollervey -- Emerging technologies to study long non-coding RNAs / Fereshteh Jahaniani ... [et al.]
  • Head and neck cancers, involving sites from the nasopharynx to the subglottic larynx, are frequently devastating cancers that afflict patients around the world. These cancers are frequently locally advanced prior to detection, and require multimodality therapy that is associated with high morbidity. As this book addresses this difficult disease, it accomplishes three main goals. First, it introduces the etiology and subclasses of squamous cell carcinomas of the head and neck (SCCHNs), and how these factors affect prognosis. Although habitual exposures to tobacco, alcohol, and other agents have historically been the main causes of SCCHN, a rising proportion of oropharynx cancers arise from transforming human papillomavirus (HPV) infection. These two broad classes of SCCHN have significant differences in disease profile and response to treatment, as we discuss. Second, it summarizes the current state of understanding of the genetic, epigenetic and protein expression changes associated with the various classes of SCCHN. In the past decade, disease pathogenesis of SCCHN has been appreciated to involve deregulation of multiple tumor pathways, including the receptor tyrosine kinases (RTKs) EGFR, ERBB2, ERBB3, c-MET, and IGF1R; transforming growth factor β (TGFβ); Notch; cytoplasmic signaling proteins including PTEN, PI3K, JAK/STAT, and Wnt-responsive β-catenin; mutation control systems, including p53 and the DNA damage repair (DDR) machinery; and hypoxic response. The specific understanding of the action of these proteins in SCCHN is presented here. Finally, this book defines potential therapeutic targets for improved management of the disease in the future, discussing prospects for improved prediction of prognosis.
  • Current and emerging approaches to studying invasion in apicomplexan parasites / Jeffrey Mital and Gary E. Ward -- Microneme proteins in apicomplexans / Vern B. Carruthers and Fiona M. Tomley -- Erythrocyte invasion by plasmodium falciparum: multiple ligand-receptor interactions and phenotypic switching / Manoj T. Duraisingh ... [et al.] -- Role of the gp85/trans-sialidase superfamily of glycoproteins in the interaction of trypanosoma cruzi with host structures / Maria Jølia M. Alves and Walter Colli -- Calcium regulation and signaling in apicomplexan parasites / Kisaburo Nagamune ... [et al.] -- Trypanosoma cruzi: parasite and host cell signaling during the invasion process / Nobuko Yoshida and Mauro Cortez -- Host cell actin remodeling in response to cryptosporidium / Steven P. O'Hara ... [et al.] -- Host cell actin remodeling in response to trypanosoma cruzi: trypomastigote versus amastigote entry / Renato A. Mortara ... [et al.] -- Actin/myosin-based gliding motility in apicomplexan parasites / Kai Matuschewski and Herwig Schøler -- Roles of proteases during invasion and egress by plasmodium and toxoplasma / Timothy J. Dowse ... [et al.] -- Roles of naturally occurring protease inhibitors in the modulation of host cell signaling and cellular invasion by trypanosoma cruzi / Julio Scharfstein and Ana Paula C.A. Lima -- Biogenesis of and activities at the toxoplasma gondii parasitophorous vacuole membrane / Anthony P. Sinai. The role of host cell lysosomes in trypanosoma cruzi invasion / G. Adam Mott and Barbara A. Burleigh -- Leishmania invasion and phagosome biogenesis / Robert Lodge and Albert Descoteaux -- Plasmodium sporozoite passage across the sinusoidal cell layer / Ute Frevert ... [et al.] -- Transepithelial migration by toxoplasma / Antonio Barragan and Niclas Hitziger -- The microsporidian polar tube and its role in invasion / Frederic Delbac and Valerie Polonais -- Intestinal invasion by entamoeba histolytica / Shahram Solaymani-Mohammadi and William A. Petri, Jr.
  • Molecular modeling of proteins 2008, Springer Protocols
    Molecular dynamics simulations / Erik R. Lindahl -- Monte Carlo simulations / David J. Earl and Michael W. Deem -- Hybrid quantum and classical methods for computing kinetic isotope effects of chemical reactions in solutions and in enzymes / Jiali Gao ... [et al.] -- Comparison of protein force fields for molecular dynamics simulations / Olgun Guvench and Alexander D. MacKerell, Jr. -- Normal modes and essential dynamics / Steven Hayward and Bert L. de Groot -- Calculation of absolute protein-ligand binding constants with the molecular dynamics free energy perturbation method / Hyung-June Woo -- Free energy calculations applied to membrane proteins / Christophe Chipot -- Molecular dynamics simulations of membrane proteins / Philip C. Biggin and Peter J. Bond -- Membrane-associated proteins and peptides / Marc F. Lensink -- Implicit membrane models for membrane protein simulation / Michael Feig -- Comparative modeling of proteins / Gerald H. Lushington -- Transmembrane protein models based on high-throughput molecular dynamics simulations with experimental constraints / Andrew J. Beevers and Andreas Kukol -- Nuclear magnetic resonance-based modeling and refinement of protein three-dimensional structures and their complexes / Gloria Fuentes, Aalt D.J. van Dijk, and Alexandre M.J.J. Bonvin -- Conformational changes in protein function / Haiguang Liu ... [et al.] -- Protein folding and unfolding by all-atom molecular dynamics simulations / Hongxin Lei and Yong Duan -- Modeling of protein misfolding in disease / Edyta B. Małolepsza -- Identifying putative drug targets and potential drug leads : starting points for virtual screening and docking / David S. Wishart -- Receptor flexibility for large-scale in silico ligand screens : chances and challenges / B. Fischer, H. Merlitz, and W. Wenzel -- Molecular docking / Garrett M. Morris and Marguerita Lim-Wilby.
  • Part 1. Structure and Function of Mortalin / Birth of Mortalin: Multiple Names, Niches and Functions Connecting Stress, Senescence and Cancer / Renu Wadhwa and Sunil C. Kaul -- Mortalin's Machinery / Custer C. Deocaris, Sunil C. Kaul and Renu Wadhwa -- The Role of Mortalin in Iron Homeostasis / Wen-I Luo and James A. Cowan -- Functional Characteristics of Mortalin / Walter A. Baseler, Tara L. Croston and John M. Hollander -- Part 2. Mortalin in Evolution2 Deocaris / Mortalin and Drosophila DmHsp22: Two Mitochondrial Chaperones Regulating Aging and Carcinogenesis / Marie Le Pécheur, Geneviève Morrow and Robert M. Tanguay -- Mortalin in Invertebrates and The Induction of Apoptosis by Wild-Type p53 Following Defeat of Mortalin-Based Cytoplasmic Sequestration in Cancerous Clam Hemocytes / Charles W. Walker, Ben Low and S. Anne Böttger -- Mortalin and Stem Cells: A Study from Planarians / Renata Batistoni -- Part 3. Mortalin in Health and Disease / Mortalin in Cell Protection from Immune Attack / Moran Saar, Oren Moskovich and Zvi Fishelson -- Mortalin in Neurological Diseases / Jinghua Jin, Jing Zhang, Travis J. Cook and Jake G. Hoekstra -- Loss of Mortalin Function in Parkinson's Disease-Supporting the Mitochondrial Pathway of Neurodegeneration / Lena F. Burbulla and Rejko Krüger -- Hsp75/mortalin and Protection from Ischemic Brain Injury / Robin E. White, Yi-Bing Ouyang and Rona G. Giffard -- Catecholamine Regulated Protein (CRP40), A Splice Variant of Mortalin-2: Functional Role in CNS Disorders / Joseph P. Gabriele, Sarah E. Groleau, Ritesh P. Daya, Zdenek B. Pristupa and Ram K. Mishra -- Chaperonopathies: Diseases in Which Mortalin and Other Hsp-Chaperones Play a Role in Etiology and Pathogenesis / Alberto J. L. Macario, Francesco Cappello and Everly Conway de Macario -- Part 4. Mortalin and Cancer / Many Faces of Mortalin and Tid1 / Ohad Iosefson and Abdussalam Azem -- Mortalin: A Positive Regulator of Centrosome Duplication and Amplification / Masayuki Kanai and Kenji Fukasawa -- Mortalin Expression in Normal and Neoplastic Tissues / Angheliki Nomikos, Sinclair R. Dundas and Graeme I. Murray -- Mortalin-p53 Interaction as a Target for Liver Cancer Therapy / Wen-Jing Lu, Nikki P. Lee, Renu Wadhwa and John M. Luk -- Mortalin Targeting Gadgets for Cancer Therapy / Chae-Ok Yun and Renu Wadhwa -- Part 5. Mortalin Based Technologies / Cell Internalizing Anti-Mortalin Antibody for Generation of Illuminating MSCs for Long-Term In vitro and In vivo Tracking / Toshimasa Uemura, Masanori Nishi, Sunil C. Kaul and Renu Wadhwa -- Mortalin Staining Pattern as a Reporter for Cell Based Anti-Cancer Drug Screening / Ran Gao, Zeenia Kaul, Tomoko Yaguchi and Renu Wadhwa -- Cell Internalizing Anti-mortalin Antibody as a Nanocarrier / Zeenia Kaul, Tomoko Yaguchi, Renu Wadhwa and Sunil C. Kaul.
  • mTOR inhibitors in cancer treatment / Leonidas C. Platanias -- Overview and mechanism of action of mTOR inhibitors / Stéphane Oudard -- mTOR inhibitors in renal cell carcinoma / Piotr J. Wysocki -- mTOR inhibitors in hepatocellular carcinoma / Gerhard Treiber & Thomas Wex -- mTOR inhibitors in ovarian cancer / Susana M. Campos -- mTOR inhibitors in breast cancer / Ingrid A. Mayer -- mTOR inhibitors in Hodgkin's and non-Hodgkin's lymphomas / Sonali M. Smith.
  • Mucins and cancer 2013, Future Med
    Mucins and cancer / Joyce Taylor-Papadimitriou & Joy M. Burchell -- Introduction to mucins in cancer / Michael A. McGuckin -- Changes in mucin-type O-linked glycosylation in cancer / Joy M. Burchell & Joyce Taylor-Papadimitriou -- Mucins as biomarkers in cancer / Kowa Chen, Ola Blixt & Hans H. Wandall -- Signaling from membrane mucins / Tze Wei Poh & Sandra J. Gendler -- MUC4 and membrane receptors in cancer / Kermit L. Carraway & Coralie A. Carothers Carraway -- MUC16, alias CA125 and ovarian cancer / Evangelia-Ourania Fourkala, Aleksandra Gentry-Maharaj & Usha Menon -- Mucins in metastasis / Sylvain Julien -- How mucins shape antigen-presenting cells to modify immune responses / Sandra J. van Vliet & Yvette van Kooyk -- MUC1 as a therapeutic target in cancer / Jean-Marc Limacher & Bruce Acres -- Index.
  • This book provides the readers with an overview of research on p53, which has been shown to play a role in numerous crucial biological pathways in normal and cancer cells. Leading scientist in the field, who have all made direct contributions to the understanding of the molecular events underpinning p53 function, have been invited to contribute the various chapters, which discuss the current knowledge of the signaling cascades that are activated by mutations in p53 and overexpression of MDM2, frequently found in human cancer, and are major causes of oncogenesis. This book features chapters on the molecular basis of oncogenesis induced by gain of function mutation of p53, signaling pathways induced by MDM2 overexpression, control of mutant or wild-type p53 function by MDM2 and MDMX, p53 mutation in hereditary cancer, and structural aspects that activate mutant p53 which can be targeted by drug therapy. This book should be useful for scientists at all levels.
  • The structural and functional diversity of the myosin family of actin-based molecular motors / M.S. Mooseker and B.J. Foth -- Myosin structure / K.C. Holmes -- The myosin family: Biochemical and kinetic properties / M. El-Mezgueldi and C.R. Bagshaw -- Myosin I / L.M. Coluccio -- Myosin II : Sarcomeric myosins, the motors of contraction in cardiac and skeletal muscles / C. Reggiani and R. Bottinelli -- Smooth-muscle myosin II / C.R. Cremo and D.J. Hartshorne -- Non-muscle myosin II / M. Conti, S. Kawamoto and R.S. Adelstein -- Class III myosins / A. Dosé, J. Lin-Jones and B. Burnside -- Myosin V / J.R. Sellers and L.S. Weisman -- Myosin VI : A multifunctional motor protein / F. Buss and J. Kendrick-Jones -- Myosin VII / A. El-Amraoui, A. Bahloul and C. Petit -- Plant myosins VIII, XI, and XIII / K. Yamamoto -- Class IX myosins / M. Bähler -- Myosin X / M.M. Divito and R.E. Cheney -- Myosin class XIV and other myosins in protists / K. Frénal, B.J. Foth and D. Soldati -- Myosin XVa / E.T. Boger ... [et al.].
  • pt. B ScienceDirect
    pt. C ScienceDirect
  • Natural products isolation. 2nd ed. 2005, Springer Protocols
    Natural product isolation : an overview / Satyajit D. Sarker, Zahid Latif, and Alexander I. Gray -- Initial and bulk extraction / Véronique Seidel -- Supercritical fluid extraction / Lutfun Nahar and Satyajit D. Sarker -- An introduction to planar chromatography / Simon Gibbons -- Isolation of natural products by low-pressure column chromatography / Raymond G. Reid and Satyajit D. Sarker -- Isolation by ion-exchange methods / David G. Durham -- Separation by high-speed countercurrent chromatography / James B. McAlpine and Patrick Morris -- Isolation by preparative high-performance liquid chromatography / Zahid Latif -- Hyphenated techniques / Satyajit D. Sarker and Lutfun Nahar -- Purification by solvent extraction using partition coefficient / Hideaki Otsuka -- Crystallization in final stages of purification / Alastair J. Florence, Norman Shankland, and Andrea Johnston -- Dereplication and partial identification of compounds / Laurence Dinan -- Extraction of plant secondary metabolites / William P. Jones and A. Douglas Kinghorn -- Isolation of marine natural products / Wael E. Houssen and Marcel Jaspars -- Isolation of microbial natural products / Russell A. Barrow -- Purification of water-soluble natural products / Yuzuru Shimizu and Bo Li -- Scale-up of natural product isolation / Steven M. Martin, David A. Kau, and Stephen K. Wrigley -- Follow-up of natural product isolation / Richard J.P. Cannell.
  • Naturally occurring IgM antibodies to oxidation-specific epitopes / Christoph J. Binder -- Naturally occurring autoantibodies to apoptotic cells / Keith B. Elkon and Gregg J. Silverman -- Naturally occurring antibodies directed against carbohydrate tumor antigens / Reinhard Schwartz-Albiez -- Naturally occurring antibodies as therapeutics for neurologic disease : can human monoclonal IgMs replace the limited resource IVIG? / Arthur E. Warrington ... [et al.] -- Nature and nurture of catalytic antibodies / Sudhir Paul ... [et al.] -- Naturally occurring autoantibodies in mediating clearance of senescent red blood cells / Hans U. Lutz -- Naturally occurring autoantibodies against ss-amyloid / Jan-Philipp Bach and Richard Dodel -- Multi-faceted role of naturally occurring autoantibodies in fighting pathogens / Hicham Bouhlal and Srini Kaveri -- Immunoregulation by naturally occurring and disease-associated autoantibodies : binding to cytokines and their role in regulation of T-cell responses / Claus H. Nielsen and Klaus Bendtzen -- Modulation of dendritic cells and regulatory T cells by naturally occurring antibodies / Jaap Kwekkeboom -- Control of B cells expressing naturally occurring autoantibodies / Jean Louis Pasquali and Thierry Martin -- Granulocyte death regulation by naturally occurring autoantibodies / Stephan Von Gunten and Hans-Uwe Simon -- Naturally occurring autoantibodies mediate ischemia/reperfusion-induced tissue injury / Sherry D. Fleming -- How immune complexes from certain IgG NAbs and any F(ab')2 can mediate excessive complement activation / Hans U. Lutz -- The natural autoantibody repertoire in newborns and adults : a current overview / Asaf Madi ... [et al.] -- Antibody polyspecificity : what does it matter? / Jordan D. Dimitrov, Anastas D. Pashov, Tchavdar L. Vassilev -- Positive and negative selection of natural autoreactive B cells / Richard R. Hardy and Kyoko Hayakawa -- Naturally occurring antibodies/autoantibodies in polyclonal immunoglobulin concentrates / Peter J. Späth and Hans U. Lutz.
  • 1. Introduction to the neurobiology of actin -- 2. The neuronal actin cytoskeleton and the protrusion of lamellipodia and filopodia -- 3. Regulation of actin filaments during neurite extension and guidance -- 4. Functions of myosin motor proteins in the nervous system -- 5. Microtubule-actin interactions during neuronal development -- 6. Working with actin: methodological approaches for the study of actin in neurons -- 7. Functions of the actin cytoskeleton in the early embryology of the nervous system -- 8. Actin cytoskeletal regulation in neuronal migration -- 9. Actin and neuronal polarity -- 10. Actin at the synapse: contribution to pre- and postsynaptic functions -- 11. Actin and diseases of the nervous system -- 12. Signal transduction pathways: from receptor to the actin cytoskeleton -- Index.
  • Receptor-based discovery strategies for insecticides and parasiticides: a review / Debra Woods ... [et al.] -- Nonpeptide ligands for peptidergic G protein-coupled receptors / Timothy G. Geary -- Interaction of mimetic analogs of insect kinin neuropeptides with arthropod receptors / Ronald J. Nachman and Patricia V. Pietrantonio -- Neuropeptides in helminths: occurrence and distribution / Nikki J. Marks and Aaron G. Maule -- Neuropeptide physiology in helminths / Angela Mousley ... [et al.] -- Neuropeptide gene families in Caenorhabditis elegans / Chris Li and Kyuhyung Kim -- Control of nematode parasites with agents acting on neuromusculature systems: lessons for neuropeptide ligand discovery / Richard J. Martin and Alan P. Robertson -- Neuropeptide signaling in insects / Miriam Altstein and Dick R. Nassel -- Neuropeptide physiology in insects / William G. Bendena -- Neuropeptide biology in Drosophila / Elke Clynen ... [et al.] -- Neuropeptide receptors as possible targets for development of insect pest control agents / Matthias B. Van Hiel ... [et al.].
  • "Preface: Over the past four decades, the number of peptides identified as neurotransmitters or neuromodulators in the central and peripheral nervous systems has significantly increased. These compounds, known as neuropeptides, have been recognized for playing an important role in the communication between cells in a variety of neuronal networks. Although, a considerable number of neuropeptides have been characterized so far, their quantity is limited compared to the number of precursor proteins that are actually found to be expressed in cells of the nervous system. Numerous studies have confirmed that neuropeptides are involved in a number of biological activities. These include modulation of brain reward, pain processing, and immune response, as well as neuroendocrine regulations, control of neurovegetative functions, and trophic effects. In recent years, it has become evident that the role of neuropeptides as fast-acting neurotransmitters is challenged by the observation that many of them may act as growth factors by stimulating cell proliferation and slow-acting mitogenesis. It has thus been demonstrated that a number of neuroactive peptides, such as pituitary adenylate cyclase-activating polypeptide (PACAP), adrenocorticotrophin (ACTH), opioid peptides, somatostatin, and substance P, may modulate proliferation and cell viability. Some peptides act as stimulatory factors on brain circuits involved in cognition, whereas others may act as inhibitory factors. Accordingly, many neuropeptide systems appear as important targets for neuroprotective drugs and drugs that promote neuroregeneration"--Provided by publisher.
  • Gene Expression Profiling and Regulatory Networks in Single Cells / Fumio Arai, Kentaro Hosokawa, Yoshiko Matsumoto, Hirofumi Toyama and Toshio Suda -- Cell Fate Regulatory Networks / Sonya J. Ridden and Ben D. MacArthur -- Noise in Regulatory Networks / Srinandan Dasmahapatra -- Generalised Modelling in Systems Biology / Thilo Gross -- Relative Quantification Mass Spectrometry Using iTRAQ Isobaric Tags / Richard D. Unwin, Emma Carrick and Anthony D. Whetton -- Deciphering Protein Complexes and Protein Interaction Networks for Stem Cell Pluripotency / Jianlong Wang -- Omics Era in Stem Cell Research: Data Integration of Multi-regulatory Layers / Ana Sevilla, Benjamin Trinite and Ihor R. Lemischka -- High Throughput Affinity Purification and Mass Spectrometry to Determine Protein Complex Interactions / Anna Malovannaya, Rainer B. Lanz, Bert W. O'Malley and Jun Qin -- Network Pharmacology to Aid the Drug Discovery Process / Ruth Dannenfelser, Huilei Xu, Catherine Raimond and Avi Ma'ayan -- What Can Networks Do for You? / Bernhard M. Schuldt, Franz-Josef Müller and Andreas A. Schuppert.
  • Overview -- Magnesium homeostasis -- Nutrition -- Epidemiology -- Exercise -- Metabolic syndrome -- Cardiovascular disease -- Skeletal diseases and calcium metabolism -- Kidney -- Neurology -- Magnesium in dental medicine -- Magnesium in neurology and psychiatry -- Veterinary medicine.
  • Section I: -- Chemical biology: Determinants of nitric oxide chemistry: inpact of cell signaling processes / Douglas D. Thomas, Wilmarie Flores-Santana, Christopher H. Switzer -- Nitric oxide redox biochemistry in lipid environments / Andrés Trostchansky, Matías N. Möller, Silvina Bartesaghi, Horacio Botti, Ana Denicola, Rafael Radi and Homero Rubbo -- Mechanisms and biological consequences of peroxynitrite-dependent protein oxidation and nitration / Madia Trujillo, Beatriz Alvarez, José M. Souza, Natalia Romero, Laura Castro, Leonor Thomson and Rafael Radi -- Systems approaches to unraveling nitric oxide response netwoeks in prokaryotes / Laura R. Jarboe, Daniel R. Hyduke and James C. Liao. Section II: -- Principles of biology: (A. Nitric oxide synthases) Uncoupling of endothelial nitric oxide synthase in cardiovascular disease and its pharmacological reversal / Ulrich Förstermann -- Tetrahydrobiopterin: an essential cofactor for nitric oxide syntahases and amino acid hydroxylases / Christina E. Higgins and Steven S. Gross -- Regulation of the expression of inducible nitric oxide synthase / Hartmut Kleinert, Julia Art and Andrea Pautz -- Molecular regulation of inducible nitric oxide synthase / Qiang Du and David A. Geller -- (B. Guanylate cyclase and cyclic GMP) Soluble guanylate cyclase: allosteric activtion and redox regulation / Adrian J. Hobbs and Johannes-Peter Stasch (C. Nitric oxide signaling) Untargeted discovery of nitric oxide-modified proteins / Ruba Deeb, Tal Nuriel and Steven S. Gross -- Fatty acid transduction of nitric oxide signaling: cyclooxygenases, lipoxygenases and nitro-fatty acids / Marsha P. Cole and Bruce A. Freeman -- Nitric oxide signaling in vascular cells is regulated through CD47 by thrombospondin-1 / Jeffery S. Isenberg, William A. Frazier and David D. Roberts -- The regulation of cell energetics and mitochondrial signaling by nitric oxide / Alberto Boveris, Maria Cecilia Carreras and Juan Jose Poderoso -- (D. Regulation of nitric oxide production) Nitric oxide-asymmetric dimedthylarginine system in endothelial cell senescence / Fortunato Scalera and Stefanie M. Bode-Böger -- (E. Nitric oxide and remodeling) The role of nitric oxide in apoptosis and autophagy: biochemical and computational studies / P. A. Loughran, E. Z. Bagci, R. Zamora, Y. Vodovotz and T. R. Billiar -- (F. Nitrite and nitrate) Nitric oxide formation from inorganic nitrate and nitrite / Jon O. Lundberg and Eddie Weitzberg -- Mechanisms of nitrite reduction in ischemia in the cardiovascular system / Andrew Webb and Amrita Ahluwalia -- Nitrite therapy for ischemic syndromes / Christopher G. Kevil and David J. Lefer -- Nitrite and heme globins: reaction mechanisms and physiological targets / Sruti Shiva, Sheila Frizzill and Mark T. Gladwin. Section III: -- Principles of pathobiology (A. Nitric oxide and cardiovascular function) Nitric oxide in vascular damage and regeneration / Claudio Napoli, Lilach O. Lerman, Maria Luisa Balestrieri and Louis J. Ignarro -- Free radicals as atherosclerotic risk in relation to nitric oxide / Toshio Hayashi and Akihisa -- The role of oxidative stress in endothelial dysfunction and vascular inflammation / Elena Osto and Francesco Cosentino -- (B. Pathophysiological mechanism of nitric oxide) Nitric oxide, oxidative stress, immune response and critical care / Claudio Napoli, Ettore Crimi, Sharon Williams-Ignarro, Filomena de Nigris and Louis J. Ignarro -- Reactive metabolites of oxygen and nitrogen in liver ischemia and reperfusion injury / Ian N. Hines and Matthew B. Grisham -- Nitric oxide in airway inflammation / Yong Chul Lee, So Ri Kim, Eun-Kyeong Jo, Hyun-Ock Pae and Hun-Taeg Chung -- Novel therapeutic applications of nitric oxide in the inhibition of tumor malignancy and reversal of resistance / Benajmin Bonavida.
  • Nitric oxide methods and protocols 2011, Springer Protocols
    Nitric oxide physiology and pathology / David G. Hirst and Tracy Robson -- Ionizing radiation-induced DNA strand breaks and [gamma]-H2AX foci in cells exposed to nitric oxide / Kai Rothkamm and Susanne Burdak-Rothkamm -- Determination of S-nitrosothiols in biological fluids by chemiluminescence / Enika Nagababu and Joseph M. Rifkind -- Measurement of nitrite in blood samples using the ferricyanide-based hemoglobin oxidation assay / Barbora Piknova and Alan N. Schechter -- Selective fluorescent activation for bioimaging the expression of nitric oxide in cellular and in vivo systems / Junfeng Zhang and Hao Hong -- Real-time measurement of murine hippocampus NO levels in response to cerebral ischemia/reperfusion / Xiaoxiang Zheng, Kezhou Liu, and Yong Yang -- Detection of low levels of nitric oxide using an electrochemical sensor / Yong Chool Boo ... [et al.] -- Determination of the scavenging capacity against reactive nitrogen species by automatic flow injection-based methodologies / Marcela A. Segundo ... [et al.] -- Aqueous measurement of nitric oxide using membrane inlet mass spectrometry / David N. Silverman and Chingkuang Tu -- Quantum cascade laser technology for the ultrasensitive detection of low-level nitric oxide / Angela Elia ... [et al.] -- Determination of in vivo nitric oxide levels in animal tissues using a novel spin trapping technology / Anatoly F. Vanin and Alexander A. Timoshin -- [Beta] cell protection by inhibition of iNOS through lentiviral vector-based strategies / Sean O. Hynes, Cillian McCabe, and Timothy O'Brien -- Characterization of nitric oxide delivery systems produced by various nanotechnologies / Chi H. Lee -- Nitric oxide releasing nanoparticle synthesis and characterization / George Han, Adam J. Friedman, and Joel M. Friedman -- NOS antagonism using viral vectors as an experimental strategy : implications for in vivo studies of cardiovascular control and peripheral neuropathies / Beihui Liu ... [et al.]
  • Nitric oxide. Part E-F pt. E-F, 2005-2008., ScienceDirect
    pt. F, 2008 ScienceDirect
  • Introduction / Muller Gabbri -- Biogenesis and physiology of microRNAs / Carlos A. Melo and Sonia A. Melo -- Detecting noncoding RNA expression : from arrays to next-generation sequencing / Catalina Perdomo, Joshua Campbell, and Frank Schembri -- MicroRNAs in solid tumors / Jin Wang and Subrata Sen -- MicroRNAs in hematologic malignancies / Johannes Bloehdorn ... [et al.] -- miRNAs as cancer biomarkers / Edward R. Sauter -- Unraveling the complex network of interactions between noncoding RNAs and epigenetics in cancer / Veronica Davalos and Manel Esteller -- MicroRNA SNPs in cancer / Yujing LI and Peng Jin -- Bioinformatics approaches to the study of MircoRNAs / Ravi Gupta and Ramana V. Davuluri -- Beyond miRNAs : role of other noncoding RNAs in cancer / Roxana S. Redis and George A. Calin -- Translational implication for noncoding RNA in cancer / Duaa Dakhallah, Melissa Piper, and S. Patrick Nana-Sinkam.
  • Nuclear import and export of mammalian viruses / Michael Bukrinsky -- Nuclear import of DNA / David A. Dean and Kerimi E. Gokay -- Nuclear transport research methods / Jose Omar Bustamante. Regulation of nuclear import and export of proteins in plants and its role in light signal transduction / Stefan Kircher ... [et al.] -- Nuclear export: shuttling across the nuclear pore / John A. Hanover and Dona C. Love -- Nuclear protein import: distinct intracellular receptors for different types of import substrates / David A. Jans and Jade K. Forwood -- The molecular mechanisms of mRNA export / Tetsuya Taura, Mikiko C. Siomi and Haruhiko Siomi -- Structure of the nuclear pore / Michael Elbaum -- Integral proteins of the nuclear pore membrane / Merav Cohen, Katherine L. Wilson and Yosef Gruenbaum -- Subnuclear trafficking and the nuclear matrix / Iris Meier -- Nuclear import and export signals / Toshihiro Sekimoto, Jun Katahira and Yoshihiro Yoneda -- Nuclear import of plant proteins / Glenn R. Hicks -- Nuclear import of agrobacterium T-DNA / Tzvi Tzfira, Benoit Lacroix and Vitaly Citovsky --
  • Nuclear receptor factsbook 2002, ScienceDirect
  • Nuclear receptors 2003, ScienceDirect
  • Nuclear receptors an introductory overview / C.M. Bunce and M.J. Campbell -- What does evolution teach us about nuclear receptors? / G. Markov, F. Bonneton and V. Laudet -- Functions of nuclear receptors in insect development / D. Martín -- The glucocorticoid receptor / R.H. Oakley and J.A. Cidlowski -- Estrogen receptors: Their actions and functional roles in health and disease / S. Nilsson and J.-Å. Gustafsson -- Androgen receptor / J.T. Dalton and W. Gao -- Thyroid hormone receptors / B. Vennström, H. Liu and D. Forrest -- The vitamin D receptor (NR1I1) / O. Maguire and M.J. Campbell -- Retinoic acid receptors / A. Cras, F. Guidez and C. Chomienne -- PPARs: Important regulators in metabolism and inflammation / L.M. Sanderson and S. Kersten -- Xenobiotic receptors CAR and PXR / C. Klaassen and H. Lu -- Fxr / Y. Wang ... [et al.] -- Physiological functions of TR2 and TR4 orphan nuclear receptor / S. Liu ... [et al.] -- Nuclear receptors and ATP dependent chromatin remodeling: A complex story / C.J. Burd and T.K. Archer -- Non-genomic action of sex steroid hormones / A. Migliaccio, G. Castoria and F. Auricchio -- Ligand regulation and nuclear receptor action / M. Hewison -- New insights to nuclear receptor gene regulation from analysis of their response elements in target genes / C. Carlberg -- Systems biology: Towards realistic and useful models of molecular networks / F.J. Bruggeman ... [et al.].
  • This volume on nucleic acid nanotechnology offers authoritative, up-to-date and comprehensive coverage of nanotechnological studies and applications of nucleic acids. It provides reviews of various aspects of nucleic acid nanotechnology, each written by an internationally leading expert in the field, and presents state-of-the-art and recent advances in nucleic acid synthetic modifications, nanoscale design, manipulation and current and future applications in bioengineering, medicine, electronics, genetic analysis, chemistry, molecular biology, surface and material sciences. It examines how nucleic acid research is merging with nanotechnology, allowing the nanoscale properties of nucleic acid to be exploited in performing challenging nanotechnological tasks, from nanorobotics and nanosensing to nucleic acid computing. This book will above all benefit anyone who is interested in nanotechnological concepts of nucleic acid design and applications, and offers a valuable resource for teaching these concepts. It is essential reading for a broad audience of scientists both in academia and industry who wish to expand their expertise on the potential of nucleic acid functions and applications.
  • Nucleic acid polymerases 2014, Springer
    This book provides a review of the multitude of nucleic acid polymerases, including DNA and RNA polymerases from Archea, Bacteria and Eukaryota, mitochondrial and viral polymerases, and other specialized polymerases such as telomerase, template-independent terminal nucleotidyl transferase and RNA self-replication ribozyme. Although many books cover several different types of polymerases, no book so far has attempted to catalog all nucleic acid polymerases. The goal of this book is to be the top reference work for postgraduate students, postdocs, and principle investigators who study polymerase.
  • Nucleolus 2011, Springer
  • Nucleosomes, histones & chromatin. Part A-B pt. A-B, 2012., ScienceDirect
    pt. B ScienceDirect
  • Nutrients and epigenetics 2009, CRCnetBASE
    Methylation on the nucleosome / Da Jia and Xiaodong Cheng -- Mammalian DNA methyltransferases / Michael J. Boland and Judith K. Christman -- Chromatin remodeling and histone modifications / Gavin R. Schnitzler -- Nutrients and DNA methylation / Sang-Woon Choi, Roberto Corrocher, and Simonetta Friso -- Nutrients, histone modifications, and chromatin remodeling in chronic inflammation / Barbara Delage and Roderick H. Dashwood -- Nutrients, epigenetics, and embryonic development / Craig A. Cooney -- Nutrients, epigenetics, and aging / John C. Mathers and Dianne Ford -- Nutrition, epigenetics, and cancer / Sharon A. Ross -- Conclusions and future perspectives / Sang-Woon Choi and Simonetta Friso.
  • "Incidences of inflammatory airway diseases are on the rise across the world. Existing therapeutic options are ineffective, unsafe, and expensive, and severe cases are nonresponsive to conventional therapy. Therefore, it is imperative that research be undertaken to discover new treatment options. Obstructive Airway Diseases: Role of Lipid Mediators discusses clinically successful and potential lipid targets that can make a difference in treating some of the most intractable disease states."--Publisher description.
  • Organelle proteomics 2008, Springer Protocols
    Analysis of organelles by on-line two-dimensional liquid chromatography-tandem mass spectrometry / Edwin P. Romijn and John R. Yates III -- Purification and proteomic analysis of chloroplasts and their sub-organellar compartments / Daniel Salvi ... [et al.] -- Proteomic analysis of the stacked golgi complex / Mark S. Lowenthal, Kathryn E. Howell, and Christine C. Wu -- Purification of Saccharomyces cerevisiae mitochondria by zone electrophoresis in a free flow device / Hans Zischka ... [et al.] -- Preparation of respiratory chain complexes from Saccharomyces cerevisiae wild-type and mutant mitochondria : activity measurement and subunit composition analysis / Claire Lemaire and Geneviève Dujardin -- Mitochondrial proteomics : analysis of a whole mitochondrial extract with two-dimensional electrophoresis / Thierry Rabilloud -- Purification and proteomic analysis of the mouse liver mitochondrial inner membrane / Sandrine Da Cruz and Jean-Claude Martinou -- Subcellular fractionation and proteomics of nuclear envelopes / Laurence Florens, Nadia Korfali, and Eric C. Schirmer -- Purification and proteomic analysis of a nuclear-insoluble protein fraction / Tsuneyoshi Horigome, Kazuhiro Furukawa, and Kohei Ishii -- Preparation methods of human metaphase chromosomes for their proteome analysis / Kiichi Fukui, Hideaki Takata, and Susumu Uchiyama -- Purification and proteomic analysis of plant plasma membranes / Erik Alexandersson ... [et al.] -- Protocol to enrich and analyze plasma membrane proteins from frozen tissues / Jacek R. Wiśniewski -- Proteomic characterization of membrane protein topology / Adele R. Blackler and Christine C. Wu -- Free flow isoelectric focusing : a method for the separation of both hydrophilic and hydrophobic proteins of rat liver peroxisomes / Markus Islinger and Gerhard Weber -- Use of gas-phase fractionation to increase protein identifications : application to the peroxisome / Jacob Kennedy and Eugene C. Yi -- Purification and proteomic analysis of lysosomal integral membrane proteins / Huiwen Zhang ... [et al.] -- Affinity purification of soluble lysosomal proteins for mass spectrometric identification / Sylvie Kieffer-Jaquinod ... [et al.] -- Purification and proteomic analysis of synaptic vesicles / Holly D. Cox and Charles M. Thompson -- Purification and proteomics analysis of pancreatic zymogen granule membranes / Xuequn Chen and Philip C. Andrews -- Isolation and proteomic analysis of chlamydomonas centrioles / Lani C. Keller and Wallace F. Marshall -- Purification and proteomic analysis of 20S proteasomes from human cells / Marie-Pierre Bousquet-Dubouch ... [et al.] -- Characterization of E. coli ribosomal particles : combined analysis of whole proteins by mass spectrometry and of proteolytic digests by liquid chromatography-tandem mass spectrometry / Isabelle Iost ... [et al.] -- Assessment of organelle purity using antibodies and specific assays : the example of the chloroplast envelope / Daniel Salvi ... [et al.] -- Identifying bona fide components of an organelle by isotope-coded labeling of subcellular fractions : an example in peroxisomes / Marcello Marelli, Alexey I. Nesvizhskii, and John D. Aitchison -- Determination of genuine residents of plant endomembrane organelles using isotope tagging and multivariate statistics / Kathryn S. Lilley and Tom P.J. Dunkley -- Quantitative proteomic analysis to profile dynamic changes in the spatial distribution of cellular proteins / Wei Yan, Daehee Hwang, and Ruedi Aebersold -- Use of transcriptomic data to support organelle proteomic analysis / Wallace F. Marshall.
  • The road to hydrogenosomes -- Mitochondria: key to complexity -- Origin, function,and transmission of mitochondria -- Mitochondria and their host: morphology to molecular phylogeny -- Anaerobic mitochondria: properties and origins -- Iron-sulfur proteins and iron-sulfur cluster assembly in organisms with hydrogenosomes and mitosomes -- Hydrogenosomes (and related organelles, either) are not the same -- The chimeric origin of mitochondria: photosynthetic cell enslavement, gene-transfer pressure, and compartmentation efficiency -- Constantin Merezhkowsky and the endokaryotic hypothesis -- The diversity of mitochondrion-related organelles amongst eukaryotic microbes -- Mitosomes of parasitic protozoa: biology and evolutionary significance -- index.
  • Osmosensing and osmosignaling 2007, ScienceDirect
    Water compartments in cells / G. D. Fullerton ... [et al.] -- Yeast osmoregulation / S. Hohmann ... [et al.] -- Physiological analysis of bacterial mechanosensitive channels / I. R. Booth ... [et al.] -- Control of cell cycle in response to osmostress: Lessons from yeast / J. Clotet ... [et al.] -- Bacterial osmosensing transporters / J. M. Wood -- Plant gene networks in osmotic stress response: From genes to regulatory networks / L. S. Tran ... [et al.] -- Osmosensing by integrins in rat liver / F. Schliess ... [et al.] -- Hyperosmotic activation of the CD95 system / R. Reinehr ... [et al.] -- New approaches for determining apoptotic volume decrease in cells / C. D. Bortner ... [et al.] -- Transient receptor potential channels in mechanosensing and cell volume regulation / S. F. Pedersen ... [et al.] -- Cell volume regulatory ion channels in cell proliferation and cell death / F. Lang ... [et al.] -- Actin cytoskeleton architecture and signaling in osmosensing / E. A. Papakonstanti ... [et al.] -- Osmotic stress and DNA damage / N. I. Dmitrieva ... [et al.] -- Transcriptional activator tone-binding protein in cellular protection and differentiation / J. A. Kim ... [et al.] -- Desiccation response of mammalian cells: Anhydrosignaling / Z. Huang ... [et al.] -- Tonicity-regulated gene expression / J. D. Ferraris ... [et al.] -- Hyperosmotic induction of mitogen-activated protein kinase scaffolding / T. L. Hilder ... [et al.] -- Osmoregulation of bile formation / R. Kubitz ... [et al.] -- Osmosignaling and volume regulation in intestinal epithelial cells / C. H. Lim ... [et al.] -- Osmotic regulation of cellular glucose uptake / P. Gual ... [et al.] -- Effects of osmolytes on protein folding and aggregation in cells / Z. Ignatova ... [et al.] -- Simulations of macromolecules in protective and denaturing osmolytes: Properties of mixed solvent systems and their effects on water and protein structure and dynamics / D. A. Beck ... [et al.] -- Application of the transfer model to understand how naturally occurring osmolytes affect protein stability / M. Auton ... [et al.] -- Mechanisms of high salinity tolerance in plants / N. Tuteja -- Phenotype of the taurine transporter knockout mouse / U. Warskulat ... [et al.] -- Molecular basis of osmolyte effects on protein and metabolites / J. Rosgen -- Methods of changing biopolymer volume fraction and cytoplasmic solute concentrations for in vivo biophysical studies / M. C. Konopka ... [et al.] -- Characterization of plant aquaporins / R. Kaldenhoff ... [et al.].
  • Oxidatively generated damage to cellular DNA : mechanistic aspects / Jean Cadet ... [et al.] -- Chlorination and nitration of DNA and nucleic acid components / Clare L. Hawkins ... [et al.] -- Prevention of the mutagenicity and cytotoxicity of oxidized purine nucleotides / Yusaku Nakabeppu ... [et al.] -- Nucleotide incision repair : an alternative and ubiquitous pathway to handle oxidative DNA damage / Sophie Couvé-Privat ... [et al.] -- OGG1 : from structural analysis to the knockout mouse / Arne Klungland, Jon K. Laerdahl, and Torbjörn Rognes -- Processing of 3'-end modified DNA strand breaks induced by oxidative damage / Jason L. Parsons, Emma Boswell, and Grigory L. Dianov -- Oxidative damage and promoter function / David Mitchell and Rita Ghosh -- Oxidative DNA damage and telomere shortening / Torsten Richter and Thomas von Zglinicki -- Oxidative damage and repair in the mitochondrial genome / Lene Juel Rasmussen and Keshav K. Singh -- The role of oxidative damage to nucleic acids in the pathogenesis of neurological disease / V. Prakash Reddy ... [et al.] -- Nucleic acid oxidation and the pathogenesis of cardiovascular diseases / Maria Grazia Andreassi -- Oxidative DNA damage and carcinogenesis / Ryszard Olinski, Marek Foksinski, and Barbara Tudek -- The physiological and pathological roles of oxidative damage to DNA in relation to life stage / Alberto Izzotti -- Analysis of 8-hydroxy-2'-deoxyguanosine as a marker of oxidatively damaged DNA in relation to carcinogenesis and aging / H. Kasai ... [et al.] -- Oxidatively damaged DNA and inflammation / Peter C. Dedon and Marita C. Barth -- The role of antioxidants in the prevention of oxidative damage to nucleic acids / Peter Muller and Steffen Loft.
  • Oxidative stress and disease v. 6-8, 10-22, 29-, 2001-02, 2004-06, 2012-, CRCnetBase
  • Many physiological conditions such as host defense or aging and pathological conditions such as neurodegenerative diseases, and diabetes are associated with the accumulation of high levels of reactive oxygen species and reactive nitrogen species. This generates a condition called oxidative stress. Low levels of reactive oxygen species, however, which are continuously produced during aerobic metabolism, function as important signaling molecules, setting the metabolic pace of cells and regulating processes ranging from gene expression to apoptosis. For this book we would like to recruit the experts in the field of redox chemistry, bioinformatics and proteomics, redox signaling and oxidative stress biology to discuss how organisms achieve the appropriate redox balance, the mechanisms that lead to oxidative stress conditions and the physiological consequences that contribute to aging and disease.
  • P53 in the clinics 2013, Springer
    TP53: Coordinator of the Processes That Underlie the Hallmarks of Cancer / Pierre Hainaut -- The Inheritance of p53 / Lukasz F. Grochola, Jorge Zeron-Medina, Emmanouela Repapi, Alexander E. Finlayson and Ying Cai, et al. -- p53: Guardian of the Metabolome / Masha V. Poyurovsky and Carol Prives -- The p53 Family and Stem Cell Biology / Massimiliano Agostini, Alessandro Rufini, Edward T. W. Bampton, Francesca Bernassola and Gerry Melino, et al. -- Mutant p53-Driven Tumorigenesis / Tamara Terzian and Guillermina Lozano -- Humanised Mouse Models: Targeting the Murine p53 Locus with Human Sequences / Monica Hollstein and Yang Xu -- p53 Models for Mammary Carcinogenesis / Wolfgang Deppert and Genrich Tolstonog -- TP53 Somatic Mutations: Prognostic and Predictive Value in Human Cancers / Magali Olivier -- Assessing TP53 Status in Human Tumors: Lessons from Breast Cancer / Anita Langerød, Magali Olivier and Anne-Lise Børresen-Dale -- TP53 Germline Mutations: Genetics of Li-Fraumeni Syndrome / Doua Bakry and David Malkin -- Tp53 Gene Therapy for Cancer Treatment and Prevention / Robert E. Sobol, Yong-Song Guan, Long-Jiang Li, Wei-Wei Zhang and Zhaohui Peng, et al. -- Upstream Targets in the p53 Pathway / Anna R. McCarthy and Sonia Lain -- p53-Reactivating Molecules as Research Tools and Anticancer Drugs / Vera V. Grinkevich, Andreas Warnecke and Galina Selivanova -- Targeting Mutant p53 for Improved Cancer Therapy / Jinfeng Shen, Vladimir J. N. Bykov and Klas G. Wiman -- p53 Immunotherapy of Cancer / Hakim Echchannaoui and Matthias Theobald -- Designing p53 Trials: A Surgical Oncologists View / Daniela Kandioler, Sonja Kappel and Brigitte Wolf -- p53 in the Clinic: A Pathologist's View / Philippe Bertheau, Jean-François Fléjou, Yves Allory, Pascale Varlet and Sylvie Lantuejoul, et al. -- Genetic Counseling for TP53 Germline Mutations / Maria Isabel Waddington Achatz and Patricia Ashton-Prolla.
  • 1. Functional maturation of PAKs from unicellular to multicellular organisms / Masato Okada, Graham Cote, Ramesh K. Jha, and Hiroshi Maruta -- 2. Oncogenicity of PAKs and their substrates / Hong He and Hiroshi Maruta -- 3. Natural or synthetic therapeutics that block PAKs / Hiroshi Maruta, Shanta M. Messerli, and Ramesh K. Jha -- 4. PAK1-3 in infectious diseases / Hiroshi Maruta -- 5. PAK1 in brain diseases or disorders / Hiroshi Maruta and Shanta M. Messerli -- 6. PAK1 in Alzheimer's and Huntington's diseases / Qui-Lan Ma, Fusheng Yang, Sally A. Frautschy, and Greg M. Cole -- 7. PAK1 controls the lifespan / Sumino Yamase and Hiroshi Maruta -- 8. 3D structure and physiological regulation of PAKs / Stefan Knapp.
  • "A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution"--Provided by publisher.
  • Peptide and protein delivery 2011, ClinicalKey
    The growing area of peptide and protein therapeutics research is of paramount importance to medical application and advancement. A needed reference for entry level researchers and researchers working in interdisciplinary. Covering cerebral delivery, pulmonary delivery, transdermal delivery, intestinal delivery, ocular delivery, parenteral delivery, and nasal delivery, this resource offers an overview of the main routes in therapeutics. Researchers across biochemistry, pharmaceutical, molecular biology, cell biology, immunology, chemistry and biotechnology fields will find this publication invaluable for peptide and protein laboratory research. Covers current and emerging routes for delivery of therapeutics with a focus on end application Covers Comparative Effectiveness Research Concise, accessible information from active researchers in academia and industry.
  • HPLC analysis and purification of peptides / Colin T. Mant ... [et al.] -- Identification of proteins based on MS/MS spectra and location of posttranslational modifications / Kathryn L. Stone ... [et al.] -- Determination of phosphorylated and O-glycosylated sites by chemical targeting (CTID) at ambient temperature / Gary M. Hathaway -- Synthesis, biosynthesis, and characterization of transmembrane domains of a G protein-coupled receptor / Fred Naider -- Application of topologically constrained mini-proteins as ligands, substrates, and inhibitors / Janelle L. Lauer-Fields ... [et al.] -- Proteolytic profiling of the extracellular matrix degradome / Diane Baronas-Lowell ... [et al.] -- [Beta]-amyloid protein aggregation / Marcus A. Etienne ... [et al.] -- Radiometal-labeled somatostatin analogs for applications in cancer imaging and therapy / Jason S. Lewis and Carolyn J. Anderson -- Cell-penetrating proline-rich peptidomimetics / Josep Farrera-Sinfreu ... [et al.] -- Peptide-mediated targeting of liposomes to tumor cells / Evonne M. Rezler ... [et al.] -- Peptide-mediated delivery of nucleic acids into mammalian cells / Sébastien Deshayes ... [et al.] -- Broth microdilution antibacterial assay of peptides / Laszlo Otvos and Mare Cudic -- Depsipeptide synthesis / Maciej Stawikowski and Predrag Cudic.
  • PNAs as novel cancer therapeutics / Luca Mologni and Carlo Gambacorti-Passerini -- Medicinal chemistry of plasmid DNA with peptide nucleic acids : a new strategy for gene therapy / Olivier Zelphati ... [et al.] -- Locked nucleic acids (LNA) and medical applications / Henrik Ørum, Andreas Wolter and Lars Kongsbak -- Peptide nucleic acids as agents to modify target gene expression and function / Gan Wang and Peter M. Glazer -- Peptide nucleic acids : cellular delivery and recognition of DNA and RNA targets / David R. Corey -- Receptor-specific targeting with complementary peptide nucleic acids conjugated to peptide analogs and radionuclides / Eric Wickstrom, Mathew L. Thakur and Edward R. Sauter -- Morpholinos and PNAs compared / James E. Summerton -- Chemistry of locked nucleic acids (LNA) : design, synthesis and bio-physical properties / Jesper Wengel ... [et al.] -- Recent applications of RNA interference (RNAi) in mammalian systems / Lisa Scherer and John J. Rossi -- Peptide nucleic acids as epigenetic inhibitors of HIV-1 / Shizuko Sei -- Therapeutic uses of peptide nucleic acids (PNA) in oncology / Nadia Zaffaroni, Raffaella Villa and Marco Folini -- The many faces of PNA / Peter E. Nielsen -- Modulation of nucleic acid information processing by PNAs : potential use in anti-viral therapeutics / Lionel Bastide, Bernard Lebleu and Ian Robbins -- Targeted gene delivery : the role of peptide nucleic acid / Kenneth W. Liang, Feng Liu and Leaf Huang -- Imaging gene expression in the brain with peptide nucleic acid (PNA) antisense radiopharmaceuticals and drug targeting technology / Ruben J. Boado and William M. Pardridge -- The use of PNAs and their derivatives in mitochondrial gene therapy / Paul M. Smith ... [et al.] -- Gene silencing through RNA interference: potential for therapeutics and functional genomics / David O. Azorsa, Spyro Mousses and Natasha J. Caplen -- Transcriptional activation of human CREB gene promoter using bis-PNA (peptide nucleic acid) / Christopher G. Janson ... [et al.].
  • Peptide receptors Part 1-2. 1st ed. pt. 1-2, 2000-2003., ScienceDirect
    pt. 2 (2003) ScienceDirect
  • Peptide research protocols endothelin 2002, Springer Protocols
  • Introduction -- History of PPAR Discovery -- Peroxisome Proliferator-Activated Receptors -- PPAR Ligands -- Tissue Distribution and Versatile Functions of PPARs -- PPARs and Drug Metabolism -- Molecular Aspects of PPAR Actions -- Animal Models in PPAR Research -- Safety of PPAR Agonists -- The Future of PPAR Research.
  • Peroxisomes are a class of ubiquitous and dynamic single membrane-bounded cell organelles, devoid of DNA, with an essentially oxidative type of metabolism. In recent years it has become increasingly clear that peroxisomes are involved in a range of important cellular functions in almost all eukaryotic cells. In higher eukaryotes, including humans, peroxisomes catalyze ether phospholipids biosynthesis, fatty acid alpha-oxidation, glyoxylate detoxification, etc, and in humans peroxisomes are associated with several important genetic diseases.
  • v. 2, 2011 Springer
    Vol. 1 / (ix, 306 p.) -- v. 2. / (x, 301 p.).
  • The Phosphatidylinositol 4-Kinases: Don't Call it a Comeback / Shane Minogue and Mark G. Waugh -- PIP Kinases from the Cell Membrane to the Nucleus / Mark Schramp, Andrew Hedman, Weimin Li, Xiaojun Tan and Richard Anderson -- The Phospholipase C Isozymes and Their Regulation / Aurelie Gresset, John Sondek and T. Kendall Harden -- Phosphoinositide 3-Kinases-A Historical Perspective / Alex Toker -- PI3Ks-Drug Targets in Inflammation and Cancer / Matthias Wymann -- Phosphoinositide 3-Kinases in Health and Disease / Alessandra Ghigo, Fulvio Morello, Alessia Perino and Emilio Hirsch -- Phosphoinositide Phosphatases: Just as Important as the Kinases / Jennifer M. Dyson, Clare G. Fedele, Elizabeth M. Davies, Jelena Becanovic and Christina A. Mitchell -- The PTEN and Myotubularin Phosphoinositide 3-Phosphatases: Linking Lipid Signalling to Human Disease / Nicholas R. Leslie, Elizabeth M. Davies, David A. Sheffield, Priyanka Tibarewal and Clare G. Fedele, et al.
  • Phosphoinositides play a major role in cellular signaling and membrane organization. During the last three decades we have learned that enzymes turning over phosphoinositides control vital physiological processes and are involved in the initiation and progression of cancer, inflammation, neurodegenerative, cardiovascular, metabolic disease and more. In two volumes, this book elucidates the crucial mechanisms that control the dynamics of phosphoinositide conversion. Starting out from phosphatidylinositol, a chain of lipid kinases collaborates to generate the oncogenic lipid phosphatidylinositol.
  • Phospholipases generate lipid signaling molecules through their hydrolytic action on phospholipids and are known to regulate function of a variety of cells under normal and diseased conditions. While several physiological, biochemical and molecular techniques have identified key players involved in different disease processes, phospholipases have also emerged as critical players in the pathogenesis of a number of different diseases including cancer and heart disease. In addition, phospholipases are also implicated in such conditions as brain disorder/injury, kidney and immune cell dysfunction. Phospholipases in Health and Disease is a compilation of review articles dedicated to the study of the field with respect to biochemical and molecular mechanisms of normal and abnormal cell function. The wide range of area covered here is of interest to basic research scientists, clinicians and graduate students, who are engaged in studying pathophysiological basis of a variety of diseases. Furthermore, this book highlights the potential of the different phospholipases as therapeutic targets as well as part of prevention strategies. Twenty three articles in this book are organized in four sections that are designed to emphasize the most characterized forms of the phospholipases in mammalian cells. The first section discusses general aspect of phospholipases. Section two covers the role and function of phospholipase A in different pathophysiological conditions. The third section is focussed on phospholipase C which is believed to play a central role in transmembrane signaling. The final section covers phospholipase D which is present in a variety of different cells. The book illustrates that the activation of phospholipases is of fundamental importance in signal transduction affecting cell function. Overall, this book discusses the diverse mechanisms of phospholipase mediated signal transduction in different pathophysiological conditions and raises the possibility of specific forms of phospholipases serving as novel targets for drug development.
  • O2-Sensitive Probes Based on Phosphorescent Metalloporphyrins / Ruslan I. Dmitriev and Dmitri B. Papkovsky -- O2 Analysis on a Fluorescence Spectrometer or Plate Reader / Alexander V. Zhdanov, James Hynes, Ruslan I. Dmitriev and Dmitri B. Papkovsky -- O2 Imaging in Biological Specimens / Andreas Fercher, Alexander V. Zhdanov and Dmitri B. Papkovsky.
  • pt. 1. Principles of biophysical inquiry -- pt. 2. Foundations -- pt. 3. Building a model of biomolecular structure -- pt. 4. Function and action biological state space -- pt. 5. Methods for the measuring structure and function -- pt. 6. Physical constants.
  • Introductory Chapters -- Amino acids -- Organic acids -- Vitamins and neurotransmitter -- Energy metabolism -- Organelles -- Selected disorder -- Biochemical phenotypes of questionable clinical significance -- Profiles.
  • Effect of Lifestyle, Aging, and Phytochemicals on the Onset of Neurological Disorders -- Beneficial Effects of Extra Virgin Olive Oil (n-9 Fatty Acids) on Neurological Disorders -- Beneficial Effects of Flaxseed Oil (n-3 Fatty Acids) on Neurological Disorders -- Beneficial Effects of Flavonoids on Neurological Disorders -- Beneficial Effects of Green Tea Catechins on Neurological Disorders -- Beneficial Effects of Curcumin on Neurological Disorders -- Beneficial Effects of Resveratrol on Neurological Disorders -- Beneficial Effects of Ginkgo biloba in Neurological Disorders -- Beneficial Effects of Garlic Components on Neurological Disorders -- Beneficial Effects of Propolis on Neurological Disorders -- Perspective and Direction for Future Research on Phytochemicals in Neurological Disorders.
  • Poly(ADP-ribosyl)ation 2006, Springer
    Enzymes in poly(ADP-ribose) metabolism / Ralph G. Meyer ... [et al.] -- PARP-2: structure-function relationship / Valørie Schreiber ... [et al.] -- Targeting the poly(ADP-ribose) glycohydrolase (PARG) gene in mammals / Ulrich Cortes and Zhao-Qi Wang -- DNA damage signaling through poly(ADP-ribose) / Maria Malanga and Felix R. Althaus -- Roles of poly(ADP-ribose) metabolism in the regulation of centrosome duplication and in the maintenance of neuronal integrity / Masanao Miwa ... [et al.] -- Functional interactions of PARP-1 with p53 / Rafael Alvarez-Gonzalez ... [et al.] -- Dynamic interaction between PARP-1, PCNA and p21waf1/cip1 / Ennio Prosperi and A. Ivana Scovassi -- Parp-1 as novel coactivator of NF-kB in inflammatory disorders / Paul O. Hassa and Michael O. Hottiger -- PARP and epigenetic regulation / Paola Caiafa -- PARP and the release of apoptosis-inducing factor from mitochondria / Suk Jin Hong, Ted M. Dawson and Valina L. Dawson -- Genome degradation by DNAS1L3 endonuclease: a key PARP-1-regulated event in apoptosis / A. Hamid Boulares, Alexander G. Yakovlev and Mark E. Smulson -- NAD-metabolism and regulatory functions / Mathias Ziegler -- PARP-1 and the shape of cell death / Laszlø Virøg -- Poly(ADP-ribose) polymerase (PARP) and excitotoxicity / Domenico E. Pellegrini-Giampietro, Alberto Chiarugi and Flavio Moroni -- Poly(ADP-ribose) polymerase and ischemia-reperfusion injury / Prabal K. Chatterjee and Christoph Thiemermann -- Role of poly(ADP-ribose) polymerase activation in the pathogenesis of inflammation and circulatory shock / Csaba Szabø -- Role of poly-ADP-ribosylation in cancer development / Mitsuko Masutani ... [et al.] -- PARP inhibitors and cancer therapy / Nicola J. Curtin -- Poly(ADP-ribosyl)ation and aging / Sascha Beneke and Alexander Bürkle.
  • STAR trek: an introduction to STAR family proteins and review of quaking (QKI) / Karen Artzt and Jiang I. Wu -- The STAR family member: QKI and cell signaling / Yue Feng and Andrew Bankston -- Insights into the structural basis of RNA : recognition by STAR domain proteins / Sean P. Ryder and Francesca Massi -- Post-translational regulation of STAR proteins and effects on their biological functions / Claudio Sette -- Expression and fuctions of the STAR proteins Sam68 and T-STAR in mammalian spermatogenesis / Ingrid Ehrmann and David J. Elliott -- The role of quaking in mammalian embryonic development / Monica J. Justice and Karen K. Hirschi -- Drosophila STAR proteins: what can be learned from flies? / Talila Volk -- C. elegans STAR proteins, GLD-1 and ASD-2, regulate specific RNA targets to control development / Min-Ho Lee and Tim Schedl -- The branchpoint binding protein: in and out of the spliceosome cycle / Brian C. Rymond -- Reaching for the STARs: linking RNA binding proteins to diseases / Stéphane Richard.
  • (Publisher-supplied data) Post-translational modifications serve many different purposes in several cellular processes such as gene expression, protein folding and transport to appropriate cell compartment, protein-lipid and protein-protein interactions, enzyme regulation, signal transduction, cell proliferation and differentiation, protein stability, recycling and degradation. Although several-hundred different modifications are known, the significance of many of them remains unknown. The enormous versatility of the modifications which frequently alter the physico-chemical properties of the respective proteins represents an extraordinary challenge in understanding their physiological role. Since essential cellular functions are regulated by protein modifications, an improvement of current understanding of their meaning might allow new avenues to prevent and/or alleviate human and animal diseases.
  • Disulfide bond mapping by cyanylation-induced cleavage and mass spectrometry / Jiang Wu -- Detection of post-translational modifications by fluorescent staining of two-dimensional gels / Archana M. Jacob and Chris W. Turck -- Identification of protein phosphorylation sites by advanced LC-ESI-MS/Ms methods / Christoph Weise and Christof Lenz -- Analysis of tyrosine-O-sulfation / Jens R. Bundegaard ... [et al.] -- [alpha]-amidated peptides : approaches for analysis / Gregory P. Mueller and William J. Driscoll -- [gamma]-glutamate and [beta]-hydroxyaspartate in proteins / Francis J. Castellino, Victoria A. Ploplis, and Li Zhang -- Lysine hydroxylation and cross-linking of collagen / Mitsuo Yamauchi and Masashi Shiiba -- Mass spectrometric determination of protein ubiquitination / Carol E. Parker ... [et al.] -- Analysis of sumoylation / Andrea Pichler -- Detection and analysis of protein ISGylation / Tomoharu Takeuchi and Hideyoshi Yokosawa -- Analysis of methylation, acetylation, and other modifications in bacterial ribosomal proteins / Randy J. Arnold, William Running, and James P. Reilly -- Analysis of S-acytlation of proteins / Michael Veit, Evgeni Ponimaskin, and Michael F.G. Schmidt -- Metabolic labeling and structural analysis of glycosylphosphatidylinositols from parasitic protozoa / Nahid Azzouz, Peter Gerol, and Ralph T. Schwarz -- 2-dimensional electrophoresis : detection of glycosylation and influence on spot pattern / Klemens Löster and Christoph Kannicht -- Carbohydrate composition analysis of glycoproteins by HPLC using highly fluorescent anthanilic acid (AA) tag / George N. Saddic, Shirish T. Dhume, and Kalyan R. Anumula -- Enzymatical hydrolysis of N-glycans from glycoproteins and fluorescent labeling by 2-aminobenzamide (2-AB) / Rolf Nuck -- Separation of N-glycans by HPLC / Martin Gohlke and Véronique Blanchard -- Enzymatic sequence analysis of N-glycans by exoglycosidase cleavage and mass spectrometry--detection of Lewis X structures / Christoph Kannicht, Detlef Grunow, and Lothar Lucka -- Immunochemical methods for the rapid screening of the O-glycosidically linked N-acetylglucosamine modification of proteins / Michael Ahrend ... [et al.] -- Analysis of O-glycosylation / Juan J. Calvete and Libia Sanz -- Characterization of site-specific N-glycosylation / Katalin F. Medzihradszky -- Monitoring glycosylation of therapeutic glycoproteins for consistency by HPLC using highly fluorescent anthranilic acid (AA) tag / Shirish T. Dhume, George N. Saddic, and Kalyan R. Anumula -- Comparability and monitoring immunogenic N-linked oligosaccharides from recombinant monoclonal antibodies from two different cell lines using HPLC with fluorescence detection and mass spectrometry / Bruce R.. Kilgore ... [et al.] -- Mass spectrometry and HPLC with fluorescent detection-based orthogonal approaches to characterize N-linked oligosaccharides of recombinant monoclonal antibodies / Adam W. Lucka ... [et al.] -- Web-based computational tools for the prediction and analysis of post-translational modifications of proteins / Vladimir A. Ivanisenko, Dmitry A. Afonnikov, and Nikolay A. Kolchanov.
  • Identifying transcriptional regulatory regions using reporter genes and DNA-protein interactions by chromatin immunoprecipitation / Lezanne Ooi and Ian C. Wood -- Quantitative RT-PCR methods for investigation of low copy potassium channel gene expresssion in native murine arteries / Alex Cheong, Samuel J. Fountain, and David J. Beech -- Cloning of potassium chanel splice variants from tissues and cells / Lie Chen and Michael J. Shipston -- Chemiluminescence assays to investigate membrane expression and clathrin-mediated endocytosis of KATP channels / Andrew J. Smith and Asipu Sivaprasadarao -- Investigation of KATP channel endocytosis by immunofluorescence / Andrew J. Smith and Asipu Sivaprasadarao -- Investigation of KATP channel endocytosis and cell surface density by biotinylation and western blotting / Andrew J. Smith and Asipu Sivaprasadarao -- Lipid microdomains and K+ channel compartmentation : detergent and non-detergent-based methods for the isolation and characterisation of cholesterol-enriched lipid rafts / Laura J. Sampson and Caroline Dart -- Determination of phosphoinositide binding to K+ channel subunits using a protein-lipid overlay assay / Alison M. Thomas and Andrew Tinker -- Protein complex analysis of native brain potassium channels by proteomics / Guillaume Sandoz and Florian Lesage -- Xenopus oocytes as a heterologous expression system for studying ion channels with the patch-clamp technique / Paolo Tammaro, Kenju Shimomura, and Peter Proks -- Whole-cell recording using the perforated patch clamp technique / Jonathan D. Lippiat -- Recording the activity of ATP-sensitive K+ channels in open-cell cell-attached configuration / Andrei I. Tarasov -- Planar patch clamp : advances in electrophysiology / Andrea Brüggemann ... [et al.] -- Analysing steroid modulation of BKCa channels reconstituted into planar lipid bilayers / Heidi de Wet, Jonathan D. Lippiat, and Marcus Allen -- Using bioluminescence resonance energy transfer to measure ion channel assembly / Gina M. Whitaker and Eric A. Accili -- The use of FRET microscopy to elucidate steady state channel conformational rearrangements and G protein interaction with the GIRK channels / Adi Raveh, Inbal Riven, and Eitan Reuveny -- The voltage-clamp fluorometry technique / Chris S. Gandhi and Riccardo Olcese -- Identification of mutations in the Kir6.2 subunit of the KATP channel / Sarah E. Flanagan and Sian Ellard -- Modulation of potassium ion channel proteins utilising antibodies / Mark L. Dallas, Susan A. Deuchars, and Jim Deuchars -- Fluorescence-based Tl+-influx assays as a novel approach for characterization of small-conductance CA²⁺-activated K+ channel modulators / Susanne Jørgensen, Tina H. Johansen, and Tino Dyhring -- Rubidium efflux as a tool for the pharmacological characterisation of compounds with BK channel opening properties / Neil G. McKay, Robert W. Kirby, and Kim Lawson -- Recording hERG potassium currents and assessing the effects of compounds using the whole-cell patch-clamp technique / Ray M. Helliwell.
  • Traction forces exerted by adherent cells / by Cynthia A. Reinhart-King and Daniel A. Hammer -- Control of endothelial cell adhesion by mechanotransmission from cytoskeleton to substrate / by Rosalind E. Mott and Brian P. Helmke -- Use of hydrodynamic shear stress to analyze cell adhesion / by David Boettiger -- Cellular tensegrity models and cell-substrate interactions / by Dimitrije Stamenović, Ning Wang, and Donald E. Ingber -- Bond formation during cell compression / by Elena Lomakina and Richard E. Waugh -- Dynamics of the neutrophil surface during emigration from blood / Thomas R. Gaborski and James L. McGrath -- Glycocalyx regulation of cell adhesion / Philippe Robert, Laurent Limozin, Anne-Marie Benoliel, Anne Pierres, and Pierre Bongrand -- Atomistic modeling of protein adsorption to ceramic biomaterials in water: a first step toward realistic simulation of the biomaterials surface in vivo / by Alan H. Goldstein -- Model cell membrane surfaces for measuring receptor-ligand interactions / by Craig D. Blanchette, Timothy V. Ratto, and Marjorie L. Longo -- A flow chamber for capillary networks: leukocyte adhesion in capillary sized, ligand-coated micropipettes / by David F.J. Tees, Prithu Sundd, and Douglas J. Goetz -- Adhesion of flowing neutrophils to model vessel surfaces: constraint and regulation by the local hemodynamic environment / Gerard B. Nash and G. Ed Rainger -- Hydrodynamic interactions between cells on reactive surfaces / Dooyoung Lee and Michael R. King -- Dynamics of platelet aggregation and adhesion to reactive surfaces under flow / Nipa A. Mody and Michael R. King.
  • Drs. Gerhard Meisenberg and William H. Simmons covers the latest developments in the field, including genome research, the molecular basis of genetic diseases, techniques of DNA sequencing and molecular diagnosis, and more. An updated and expanded collection of figures and access to the fully searchable text, USMLE test questions, clinical case studies and more makes this the resource for understanding all aspects of biochemistry needed in medicine. Tap into the most up-to-date coverage of new developments in genome research, the molecular basis of genetic diseases, techniques of DNA sequencing and molecular diagnosis, RNA interference as a mechanism both for regulation of gene expression and for anti-viral defense, and more. Gain a clear visual understanding through new and updated figures that provide current and relevant guidance. Make the link between basic science and clinical medicine with new Clinical Example boxes in nearly every chapter. Focus on the most essential biochemistry principles and how they apply to clinical medicine.
  • This book contains a selection of chapters aimed to provide a better understanding prion structure and biology. Together these chapters provide an overview of prion biology and underscore some of the challenges we face if we want to understand how this lively pathogen propagates and evolves in mammals. There is also mounting evidence that studying prion biology has a wider relevance due to similarities in the processes of protein misfolding and aggregation between prion disorders and other neurodegenerative disorders such as Alzheimer's, Parkinson's, and Huntington's diseases.
  • Prion protein protocols 2008, Springer Protocols
    Cell culture models to unravel prion protein function and aberrancies in prion diseases / Katarina Bedecs -- Investigation of PrPc metabolism and function in live cells : methods for studying individual cells and cell populations / Cathryn L. Haigh and David R. Brown -- Immunodetection of PrPsc using western and slot blotting techniques / Hanny Gyllberg and Kajsa Löfgren -- Assaying prions in cell culture : the standard scrapie cell assay (SSCA) and the scrapie cell assay in end point format (SCEPA) / Sukhvir P. Mahal ... [et al.] -- Generation of cell lines propagating infectious prions and the isolation and characterization of cell-derived exosomes / Laura J. Vella and Andrew F. Hill -- Neurotoxicity of prion peptides on cultured cerebellar neurons / Giuseppe D. Ciccotosto, Roberto Cappai, and Anthony R. White -- Understanding the nature of prion diseases using cell-free assays / Victoria A. Lawson -- Methods for conversion of prion protein into amyloid fibrils / Leonid Breydo, Natallia Makarava, and Ilia V. Baskakov -- Amplification of purified prions in vitro / Surachai Supattapone, Nathan R. Deleault, and Judy R. Rees -- Expression and purification of full-length recombinant PrP of high purity / Natallia Makarava and Ilia V. Baskakov -- Analysis of PrP conformation using circular dichroism / Sen Han and Andrew F. Hill -- Effect of copper on the de novo generation of prion protein expressed in Pichia pastoris / Carina Treiber -- Biophysical investigations of the prion protein using electron paramagnetic resonance / Simon C. Drew and Kevin J. Barnham -- Molecular diagnosis of human prion disease / Jonathan D.F. Wadsworth ... [et al.] -- Analysis of endogenous PrPc processing in neuronal and non-neuronal cell lines / Victoria Lewis and Steven J. Collins -- Molecular typing of PrPres in human sporadic CJD brain tissue / Victoria Lewis ... [et al.] -- Transgenic mouse models of prion diseases / Glenn C. Telling -- Quantitiative bioassay of surface-bound prion infectivity / Victoria A. Lawson.
  • Transmissible spongiform encephalopathies (TSE), now broadly known as prion diseases, have been recognized for nearly 300 years in animals and almost 100 years in humans. However, the nature of the transmissible agent had largely remained a mystery until Stanley Prusiner discovered the infectious isoform of the prion protein (PrP), named prion or scrapie PrP (PrPSc), in 1982. The subsequent modern studies with protein chemistry and molecular biology in cell culture, transgenic animals, and cell-free systems, including the revolutionary protein-misfolding cyclic amplification (PMCA), have greatly advanced our understanding of the pathogenesis of prion diseases and facilitated the identification of new prion diseases in animals and humans. In Prions and Prion Diseases, more than 60 leading researchers and clinicians worldwide provide an up-to-date development in many aspects of these unique infectious pathogens and their associated diseases.Volume I highlights the association of the cellular prion protein (PrPC) with copper and zinc, the potential roles of PrPC in Alzheimer's disease and cancers, insoluble PrPC, PMCA, molecular and cellular mechanisms of PrPSc formation and clearance, possible co-factors involved in the conversion of PrPC into PrPSc, infectious and pathogenic forms of PrP, cell biology of prions, prion strains and their interference, as well as yeast prions and their inheritable and structural traits. This unique volume covers history from the high Middle Ages to the TSE era of Daniel Carleton Gajdusek, followed by the prion era of Stanley Prusiner whose extraordinary discovery opened a new chapter in prion research. Volume I will take you through the fascinating chronicle of prions in mammals, yeast, and fungi.
  • Transmissible spongiform encephalopathies (TSE), now broadly known as prion diseases, have been recognized for nearly 300 years in animals and almost 100 years in humans. However, the nature of the transmissible agent had largely remained a mystery until Stanley Prusiner discovered the infectious isoform of the prion protein (PrP), named prion or scrapie PrP (PrPSc), in 1982. The subsequent modern studies with protein chemistry and molecular biology in cell culture, transgenic animals, and cell-free systems, including the revolutionary protein-misfolding cyclic amplification (PMCA), have greatly advanced our understanding of the pathogenesis of prion diseases and facilitated the identification of new prion diseases in animals and humans. In Prions and Prion Diseases, more than 60 leading researchers and clinicians worldwide provide an up-to-date development in many aspects of these unique infectious pathogens and their associated diseases.Volume II features a variety of animal and human prion diseases, including the newly-identified atypical forms of bovine spongiform encephalopathy and scrapie in animals, and variably protease-sensitive prionopathy in humans, prions in the environment, Tau pathology in human prion disease, transmission of the disease by blood transfusion, mammalian and non-mammalian models, conventional and advanced diagnoses, prion-specific antibodies, as well as decontamination of prions and development of therapeutics of prion diseases, such as the application of immunomodulation. This volume provides up-to-date knowledge about the etiology, pathogenesis, classification, histopathological, and clinical aspects of the highly publicized animal and human prion diseases.
  • This volume is a reference handbook focusing on diseases like Marfan syndrome, Ehlers-Danlos syndrome, Loeys-Dietz syndrome and other heritable soft connective tissue diseases. The book presents detailed information for both basic scientists and for clinicians seeing patients. It is also a stepping stone for new investigations and studies that goes beyond the facts about the composition and biochemistry of the connective tissue and extracellular matrix, as the authors connect individual components to specific aspects of various soft tissue disorders and to the actual or potential treatment of them. Progress in Heritable Soft Connective Tissue Diseases features very prominent physicians and scientists as contributors who bring their most recent discoveries to the benefit of readers. Their expertise will help clinicians with proper diagnosis of sometimes elusive and uncommon heritable diseases of soft connective tissues. This book also offers an update on the pathophysiology of these diseases, including an emphasis on unifying aspects such as connections between embryonic development of the different types of connective tissues and systems, and the role of TGF-beta in development and physiology of soft tissues. This new set of data explains, at least in part, why many of these disorders are interconnected, though the primary pathophysiological events, such as gene mutations, may be different for each disorder.
  • The book entitled "Prospects in Bioscience: Addressing the issues" is a collection of selected research papers presented at the International Conference on Advances in Biological Sciences (ICABS) organized by the Department of Biotechnology and Microbiology and the Inter University Centre for Bioscience, Kannur University, Kerala, India. ICABS witnessed a unique spectrum of Scientific Programmes on the most recent and exciting developments in modern biology. The conference displayed the numerous breakthroughs and significant developments in the important areas of modern biology and their relevance to the welfare of global society. The Book contains 50 well written chapters, each one discussing scientifically organized findings of original research work done in reputed laboratories. Needless to say, they deal with advances in various disciplines of modern biology including Cell and Molecular Biology, Structural Biology, Industrial and Environmental Biotechnology, Food and Agricultural Biotechnology and Medical Biotechnology. As the title rightly indicates, the chapters project the prospects in the respective areas and the issues in them. Specific issues discussed in the book includes development of transgenic plants, bioremediation of toxic industrial effluents, biotransformation for novel antibiotics, biofertilizer development, molecular drug designing and structure elucidation, molecular identification of pathogens, production of anti microbials, biocontrol agents and bioactive molecules, cancer biology, plant breeding and hybrid seed production etc. The book with its contents spreading across the vast arena of modern biology is expected to cater to the need of researchers, technologists and students.
  • Cell biology. Stem cells in the normal and malignant prostate / Norman J. Maitland -- Role of reactive stroma in prostate cancer / Rebeca San Martin, David R. Rowley -- The role of cholesterol in prostate cancer / Keith R. Solomon, Kristine Pelton, Michael R. Freeman -- Genetics. PTEN in prostate cancer / Marcus A. Ruscetti, Hong Wu -- ETS fusion genes in prostate cancer / J. Chad Brenner, Arul M. Chinnaiyan, Scott A. Tomlins -- Cell signaling. Signaling mechanisms of Vav3, a guanine nucleotide exchange factor and androgen receptor coactivator, in physiology and prostate cancer progression / Leah S. Lyons, Kerry L. Burnstein -- Transforming growth factor-beta in prostate cancer / David Danielpour -- The p38 MAPK pathway in prostate cancer / Daniel Djakiew -- NF-kappaB2/p52 in prostate cancer / Nagalakshmi Nadiminty, Ramakumar Tummala, Yezi Zhu -- The functional role of DAB2IP, a homeostatic factor, in prostate cancer / Eun-Jin Yun, Kaijie Wu, Yuh-Shyan Tsai -- Tyrosine kinases in prostate cancer / Yun Qiu, Dhanraj Deshmukh -- Human prostatic acid phosphatase in prostate carcinogenesis / Sakthivel Muniyan, Yu-Wei Chou, Shou-Qiang Ou-Yang -- Hormonal. Truncated androgen receptor splice variants in prostate cancer / Peter E. Lonergan, Donald J. Tindall -- Biology and clinical relevance of estrogen receptors in prostate cancer / Shuk-Mei Ho, Pheruza Tarapore, Ming-Tsung Lee, and Yuet-Kin Leung -- Vitamin D and prostate cancer / Jung-Sun Kim, Justin M. Roberts, Nancy L. Weigel -- HDAC6 regulation of androgen signaling in prostate cancer / Junkui Ai, Zhou Wang -- Beyond the cell cycle : implications of D-type cyclin deregulation in prostate cancer / Michael A. Augello, William F. Ostrander Jr., Karen E. Knudsen -- Cell death. Role of par-4 in prostate cancer / Nidhi Shukla, Nikhil K. Hebbar, Vivek M. Rangnekar -- Autophagy and prostate cancer therapeutics / Hsing-Jien Kung, Chun Changou, Hao G. Nguyen, Joy C. Yang, Christopher P. Evans, Richard J. Bold, and Frank Chuang.
  • Proteases in biology and disease v. 3-, 2005-, Springer
  • In view of rapidly growing research in the deregulation of proteases and their impact in human health and diseases, this book will highlight existing and emerging research in this exciting area. In-depth critical state-of-the-art reviews will be written by established investigators on proteases dysfunctions associated with pathogenesis of different diseases that are known to occur due to deregulation of proteolytic systems. Multidisciplinary approaches demonstrating biochemical and signal transduction mechanisms associated with deregulation of proteases leading to manifestation of the diseases.
  • "This book discusses how biological molecules exert their function and regulate biological processes, with a clear focus on how conformational dynamics of proteins are critical in this respect. In the last decade, the advancements in computational biology, nuclear magnetic resonance including paramagnetic relaxation enhancement, and fluorescence-based ensemble/single-molecule techniques have shown that biological molecules (proteins, DNAs and RNAs) fluctuate under equilibrium conditions. The conformational and energetic spaces that these fluctuations explore likely contain active conformations that are critical for their function. More interestingly, these fluctuations can respond actively to external cues, which introduces layers of tight regulation on the biological processes that they dictate. A growing number of studies have suggested that conformational dynamics of proteins govern their role in regulating biological functions, examples of this regulation can be found in signal transduction, molecular recognition, apoptosis, protein / ion / other molecules translocation and gene expression"--Publisher's description.
  • Physiological pathways of PAD activity and citrullinated epitope generation -- from citrullination to specific immunity and disease in rheumatoid arthritis -- The role of citrullinated proteins in the pathophysiology of rheumatoid arthritis -- Protein citrullination: the link between rheumatoid arthritis and periodontitis? -- From genes and environment to anti-culture immunity in rheumatoid arthritis: The role of the lungs -- Neutrophils and their contribution to autoimmunity in rheumatoid arthritis -- Deimination in skin and regulation of PAD expression in keratinocytes -- Importance of citrullination on hair protein molecular assembly during trichocytic differentiation -- Deimination in the peripheral nervous system: A wallflower existence -- Deimination in multiple sclerosis and experimental autoimmune encephalomyelitis -- Protein hypercitrullination in CNS demyelinating disease reversed by PAD inhibition -- Deimination in prion diseases -- Deimination in Alzheimer's disease -- Ongoing studies of deimination in neurodegenerative diseases using the F95 antibody -- The role of protein deimination in epigenetics -- Identifying citrullination sites by mass spectroscopy -- Homocitrulline\U+2014\an analogue and confounder related to citrulline -- Picking the PAD lock: Chemical and biological approaches to identify PAD substrates and inhibitors.
  • Protein design methods and applications 2006, Springer Protocols
    Design of structural elementary motifs. Structure and stability of the [alpha]-helix: lessons for design / Neil Errington, Teuku Iqbalsyah, and Andrew J. Doig. De novo design of monomeric [beta]-hairpin and [beta]-sheet peptides / David Pantoja-Uceda, Clara M. Santiveri, and M. Angeles Jiménez. De novo proteins from binary-patterned combinatorial libraries / Luke H. Bradley, Peter P. Thumfort, and Michael H. Hecht. Non-protein amino acids in the design of secondary structure scaffolds / Radhakrishnan Mahalakshmi and Padmanabhan Balaram. Design and synthesis of [beta]-peptides with biological activity / Marc J. Koyack and Richard P. Cheng -- Design of entire proteins and protein complexes. Design of miniproteins by the transfer of active sites onto small-size scaffolds / Franc̦ois Stricher, Loïc Martin, and Claudio Vita. Consensus design as a tool for engineering repeat proteins / Tommi Kajander, Aitziber L. Cortajarena, and Lynne Regan. Multiple sequence alignment as a guideline for protein engineering strategies / Alan R. Davidson. Sequence search methods and scoring functions for the design of protein structures / Hocine Madaoui, Emmanuelle Becker, and Raphael Guerois. Prediction of protein-protein interaction based on structure / Gregorio Fernandez-Ballester and Luis Serrano. Electrostatic design of protein-protein association rates / Gideon Schreiber, Yossi Shaul, and Kay E. Gottschalk -- Design of amyloidogenic polypeptides and amyloid inhibitors. Peptide model systems for amyloid fiber formation: design strategies and validation methods / Alexandra Esteras-Chopo, María Teresa Pastor, and Manuela López de la Paz. Protein misfolding disorders and rational design of antimisfolding agents / Lisbell D. Estrada, June Yowtak, and Claudio Soto.
  • pt. B ScienceDirect
  • Introduction -- Structure of proteins -- Physical interactions that determine the properties of proteins -- Calculation of the kinetic rate constants -- High kinetic resolution of protein folding events -- Kinetic methods for slow reactions -- Resolution of protein structures in solution -- High structural resolution of transient protein conformations -- Experimental problems of the kinetic and structural resolution of reactions that involve proteins -- The folding pathway of protein (barstar) at the resolution of individual residues from microseconds to seconds -- Highly resolved folding pathways and mechanisms of six proteins -- Structural determinants of the rate of protein folding -- Evolutionary computer programming of protein structure and folding.
  • Protein folding protocols 2006, Springer Protocols
  • Catalytic mechanisms and regulation of protein kinases / Zhihong Wang and Philip A. Cole -- A structural atlas of kinases inhibited by clinically approved drugs / Qi Wang, Julie A. Zorn, and John Kuriyan -- Fragment-based approaches to the discovery of kinase inhibitors / Paul N. Mortenson, Valerio Berdini, and Marc O'Reilly -- Targeting protein kinases with selective and semi-promiscuous covalent inhibitors / Rand M. Miller and Jack Taunton -- The resistance tetrad: amino acid hotspots for kinome-wide exploitation of drug-resistant protein kinase alleles / Fiona P. Bailey, Veselin I. Andreev, and Patrick A. Eyers -- FLiK: a direct binding assay for the identification and kinetic characterization of stabilizers of inactive kinase conformations / Jeffrey R. Simar and Daniel Rauh -- Discovery of allosteric BCR-ABL inhibitors from phenotypic screen to clinical candidate / Nathanael S. Gray and Doriano Fabbro -- The logic and design of analog-sensitive kinases and their small molecule inhibitors / Michael S. Lopez, Joseph I. Kliegman, and Kevan M. Shokat.
  • Synthesis, modification, and turnover of proteins during aging / Suresh I.S. Rattan -- Regulation of mRNA translation as a conserved mechanism of longevity control / Ranjana Mehta ... [et al.] -- Protein synthesis and the antagonistic pleiotropy hypothesis of aging / Pankaj Kapahi -- Proteasome function determines cellular homeostasis and the rate of aging / Niki Chondrogianni and Efstathios S. Gonos -- Autophagy and longevity : lessons from C. elegans / Kailiang Jia and Beth Levine -- Autophagy and aging : lessons from progeria models / Guillermo Mario, Alvaro F. Fernandez and Carlos Lopez-Oton -- Regulation of protein turnover by longevity pathways / Tibor Vellai and Krisztina Takocs-Vellai -- Protein metabolism and lifespan in Caenorhabditis elegans / Geert Depuydt, Jacques R. Vanfleteren, and Bart P. Braeckman -- Mitochondrial protein quality control systems in aging and disease / Karin Luce, Andrea C. Weil, and Heinz D. Osiewacz -- p38MAPK in the senescence of human and murine fibroblasts / Florence Debacq-Chainiaux ... [et al.] -- Protein homeostasis in models of aging and age related conformational disease / Elise A. Kikis, Tali Gidalevitz, and Richard I. Morimoto -- Roles for SUMO modification during senescence / Artemisia M. Andreou and Nektarios Tavernarakis -- Post-translational modification of cellular proteins by ubiquitin and ubiquitin-like molecules : role in cellular senescence and aging / Johannes Grillari, Regina Grillari-Voglauer, and Pidder Jansen-Dorr -- Sensory influence on homeostasis and lifespan : molecules and circuits / Joy Alcedo, Wolfgang Maier, and Queelim Ch'ng -- Regulation of muscle atrophy in aging and disease / Manlio Vinciguerra, Antonio Musaro, and Nadia Rosenthal -- Confronting cellular heterogeneity in studies of protein metabolism and homeostasis in aging research / Louise Boisen and Peter Kristensen.
  • Protein translocation across the endoplasmic reticulum membrane / Ramanujan S. Hegde -- Preprotein translocation through the sec translocon in bacteria / Antoine P. Maillard, Kenneth K.Y. Chan, and Franck Duong -- Protein translocation in archaea / Jerry Eichler -- Structure of the SecYEG protein translocation complex / Ian Collinson -- Membrane protein insertion in bacteria from a structural perspective / Mark Paetzel and Ross E. Dalbey -- The twin-arginine transport system / Frank Sargent, Ben C. Berks, and Tracy Palmer -- Retro-translocation of proteins across the endoplasmic reticulum membrane / J. Michael Lord and Lynne M. Roberts -- Chloroplast protein targeting: multiple pathways for a complex organelle / Matthew D. Smith and Danny J. Schnell -- The mitochondrial protein import machinery / Doron Rapaport -- Import of proteins into peroxisomes / Sven Thoms and Ralf Erdmann.
  • Analysis of protein phosphatases / Shirish Shenolikar -- A brief introduction to the protein phosphatase families / Tomas Mustelin -- Small molecule inhibitors of Ser/thr protein phosphatases / Mark Swingle, Li Ni, and Richard E. Honkanen -- Synthesis and use of the protein phosphatase affinity matrices microcystin- and microcystin-biotin-sepharose / Greg Moorhead, Timothy A.J. Haystead, and Carol MacKintosh -- Utilizing protein phosphatase inhibitors to define PP2A as a regulator of ataxia-telangiectasia mutated (ATM) / Aaron A. Goodarzi ... [et al.] -- An automated, fluorescence-based method for continuous assay of PP2A activity / Adam M. Wegner ... [et al.] -- An in vivo assay to quantify stable protein phosphatase 2A (PP2A) heterotrimeric species / Matthew S. Gentry, Richard L. Hallberg, and David C. Pallas -- Mutagenesis and expression of the scaffolding Aa and Ab subunits of PP2A / Ralf Ruediger, Jin Zhou, and Gernot Walter -- Isolation and characterization of PP2A holoenzymes containing FLAG-tagged B subunits / Deanna G. Adams and Brian E. Wadzinski -- Purification of PP2A holoenzymes by sequential immunoprecipitation with anti-peptide antibodies / Gernot Walter, Jin Zhou, and Ralf Ruediger -- Purification of PP2Ac from bovine heart / Hue T. Tran ... [et al.] -- Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions / Laura Trinkle-Mulcahy ... [et al.] -- Yeast two-hybrid screens to identify drosophila PP1-binding proteins / Daimark Bennett and Luke Alphey -- Identification of cellular protein phosphatase-1 regulators / David W. Roadcap, Matthew H. Brush, and Shirish Shenolikar -- Assay for 3-way interaction of protein phosphatase-1 (Glc7) with regulatory subunits plus phosphatase inhibitor-2 / Masumi Eto and David L. Brautigan -- Phosphorylation of the protein phosphatase type 1 inhibitor protein, CPI-17, by protein kinase C / Michael P. Walsh ... [et al.] -- Purification of smooth muscle myosin phosphatase using a thiophosphorylated myosin light chain-affinity resin / Merideth Borman and Justin MacDonald -- Proteins interacting with saccharomyces cerevisiae type 1 protein phosphatase catalytic subunit identified by single-step affinity purification and mass spectrometry / Edmund P. Walsh ... [et al.] -- Expression of protein histidine phosphatase in Escherichia coli, purification, and determination of enzyme activity / Nicole Bäumer .. [et al.] -- The use of RNA interference to analyze protein phosphatase function in mammalian cells / Iain Fraser ... [et al.] -- Recognition of a PP2C interaction motif in several plant protein kinases / Niranjan Chakraborty, Ohta Masaru, and Jian-Kang Zhu -- The use of yeast genetic tools to define biological roles of novel protein phosphatases / Joaquín Ariño ... [et al.] -- Targeting of PP2C in budding yeast / Irene Ota and James Mapes -- Phosphatase targets in TOR signaling / Estela Jacinto -- Functional characterization of the small CTD phosphatases (SCPs) / Michele Yeo and Patrick S. Lin -- Genome-scale discovery and characterization of class-specific protein sequences / David Kerk -- Yeast substrate-trapping system for isolating substrates of protein tyrosine phosphatases / Masahide Fukada and Masaharu Noda.
  • Protein phosphatases 2003, ScienceDirect
    Section I. Determination, detection, and localization -- Section II. Interacting proteins and subunits -- Section III. Inhibition, stimulation, and modulation of activity -- Section IV. Expression systems -- Section V. Knockdown and knockout technologies.
  • Introduction -- Elementary interactions in and around proteins -- Secondary structures of polypeptide chains -- Protein structures -- Cooperative transitions in protein molecules -- Prediction and design of protein structure -- Physical background of protein functions.
  • Quantitation of proteins -- Electrophoresis of proteins and peptides and detection in gels -- Blotting and detection methods -- Chemical modification of proteins and peptide production and purification -- Posttranslational modifications -- Antibody techniques.
  • Protein sequencing protocols. 2nd ed. 2002, Springer Protocols
  • Protein structure prediction. 2nd ed. 2008, Springer Protocols
    A historical perspective of template-based protein structure prediction / Jun-tao Guo, Kyle Ellrott, and Ying Xu -- The assessment of methods for protein structure prediction / Anna Tramontaro ... [et al.] -- Aligning sequences to structures / Liam James McGuffin -- Protein structure prediction using threading / Jinbo Xu, Feng Jiao, and Libo Yu -- Algorithms for multiple protein structure alignment and structure-derived multiple sequence alignment / Maxim Shatsky, Ruth Nussinov, and Haim J. Wolfson -- Indexing protein structures using suffix trees / Feng Gao and Mohammed J. Zaki -- Hidden Markov models for prediction of protein features / Christopher Bystroff and Anders Krogh -- The pros and cons of predicting protein contact maps / Lisa Bartoli ... [et al.] -- Roadmap methods for protein folding / Mark Moll, David Schwarz, and Lydia E. Kavraki -- Scoring functions for de novo protein structure prediction revisited / Shing-Chung Ngan ... [et al.] -- Protein-protein docking : overview and performance analysis / Kevin Wiehe ... [et al.] -- Molecular dynamics simulations of protein folding / Angel E. Garcia.
  • Introduction/overview -- Ross E. Dalbey, Gunnar von Heijne -- Methods in protein targeting, translocation and transport / Ross E. Dalbey, Minyong Chen, Martin Wiedmann -- Targeting sequences / Gunnar von Heijne -- Protein export in bacteria / Arnold J.M. Driessen, Chris van der Does -- Protein sorting at the membrane of the endoplasmic reticulum / Nora G. Haigh, Arthur E. Johnson -- Membrane protein insertion into bacterial membranes and the endoplasmic reticulum / Andreas Kuhn, Martin Spiess -- Disulfide bond formation in prokaryotes and eukaryotes / James Regeimbal, James C.A. Bardwell -- The unfolded protein response / Carmela Sidrauski, Jason H. Brickner, Peter Walter -- Protein quality control in the export pathway: the endoplasmic reticulum and its cytoplasmic proteasome connection / Zlatka Kostova, Dieter H. Wolf -- Translocation of proteins into mitochondria / Thorsten Prinz, Nikolaus Pfanner, Kaye N. Truscott -- The import and sorting of protein in chloroplasts / Jürgen Soll, Colin Robinson, Lisa Heins -- Import of proteins into peroxisomes / Suresh Subramani ... [et al.] -- Nucleocytoplasmic transport / Dirk Görlich, Stefan Jäkel -- Protein transport to the yeast vacuole / Todd R. Graham, Steven F. Nothwehr -- The secretory pathway / Benjamin S. Glick -- Vesicular transport / Joachim Ostermann, Tobias Stauber, Tommy Nilsson -- Conclusion/perspective / Ross E. Dalbey, Gunnar von Heijne.
  • Tyrosine phosphorylation is a rapid and reversible protein modification catalyzed by the yin and yang activities of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs). A multitude of PTPs have been implicated in human disease, with a growing number of PTPs now known to play major roles in prevalent metabolic diseases including obesity and type 2 diabetes. Recent studies into PTP function in the context of metabolism highlight the importance of understanding the specific substrates and binding partners of these enzymes, the regulation of PTP activity, and the cell/tissue specificity of PTP functions. This volume contains chapters which highlight many aspects of PTP function in the context of metabolism.
  • This book contains a collection of critical reviews on the expression of biologically functional proteins in Leishmania and Trypanosoma, which was written by renowned researchers on this field. Species belonging to these trypanosomatids genera are etiological agents of leishmaniasis, Chagas' disease and sleeping sickness that are extremely debilitating human infection diseases, which remain a major health problem especially in countries from Latin America, Africa and Middle East. Substantiating the problem, the currently accepted drugs for these diseases are quite unsatisfying due to their low efficacy and high toxicity. In order to solve these real problems, several research groups around the world have become involved in the study and identification of novel potential targets in the trypanosomatid cell. Since proteins are key macromolecules involved in crucial metabolic processes of all living cells, studies have focused on the expression of specific proteins produced by Leishmania and Trypanosoma by means of different biochemical, molecular and proteomic approaches in order to explore them as targets for understanding the parasite life cycle and developing new strategies against trypanosomiasis. With these proposals in mind, the book "Proteins and Proteome of Leishmania and Trypanosoma" encompasses (i) an integrated view about the biochemistry of parasites belonging to the Leishmania and Trypanosoma genera; (ii) an updated review on the expression of biologically relevant proteins by human pathogenic trypanosomatids and their possible role in the interaction with host cells/molecules as well as a target for development of both alternative chemotherapies and vaccine; and (iii) several pictures, diagrams and tables that can be used to illustrate both undergraduate and postgraduate teaching as well as scientific lectures, being a useful resource for students and researchers.
  • Energetics of peptide and protein binding to lipid membranes / William C. Wimley -- Membrane association and pore formation by alpha-helical peptides / Burkhard Bechinger -- Role of membrane lipids for the activity of pore forming peptides and proteins / Gustavo Fuertes ... [et al.] -- Cholesterol-dependent cytolysins / Robert J.C. Gilbert -- Laetiporus sulphureus lectin and aerolysin protein family / Jose Miguel Manchero .. [et al.] -- Interfacial interactions of pore-forming colicins / Helen Ridley, Christopher L. Johnson and Jeremy H. Lakey -- Permeabilization of the outer mitochondrial membrane by BCL-2 proteins / Ana J. Garcia-Suez ... [et al.] -- Molecular mechanism of sphingomyelin : specific membrane binding and pore formation by actinoporins / Biserka Bakra and Gregor Anderluh -- Hemolysin E (hlye, clya, shea) and related toxins / Stuart Hunt, Jeffrey Green and Peter J. Artymiuk -- Pore formation by cry toxins / Mario Soberin ... [et al.] -- Role of heparan sulfates and glycosphingolipids in the pore formation of basic polypeptides of cobra cardiotoxin / Wen-guey Wu ... [et al.] -- Amyloid peptide pores and the beta sheet conformation / Bruce L. Kagan and Jyothi Thundimadathil.
  • This book contains 14 original review chapters each yielding new, exciting and intriguing data about the emerging understanding of nucleolar structure and function in normal, stressed and diseased cells. The goal of this work is to provide special insight into the nucleolus of the past, present and future, as well its regulation, translocation, and biomedical function. A multitude of topics are introduced and discussed in detail, including nucleologenesis, nucleolar architecture, nucleolar targeting, retention, anchoring, translocation, and the relationship between the nucleolus and cancer.
  • Proteomic and Metabolomic Approaches to Biomarker Discovery demonstrates how to leverage biomarkers to improve accuracy and reduce errors in research. Disease biomarker discovery is one of the most vibrant and important areas of research today, as the identification of reliable biomarkers has an enormous impact on disease diagnosis, selection of treatment regimens, and therapeutic monitoring. Various techniques are used in the biomarker discovery process, including techniques used in proteomics, the study of the proteins that make up an organism, and metabolomics, the study of chemical fingerprints created from cellular processes. Proteomic and Metabolomic Approaches to Biomarker Discovery is the only publication that covers techniques from both proteomics and metabolomics and includes all steps involved in biomarker discovery, from study design to study execution. The book describes methods, and presents a standard operating procedure for sample selection, preparation, and storage, as well as data analysis and modeling. This new standard effectively eliminates the differing methodologies used in studies and creates a unified approach. Readers will learn the advantages and disadvantages of the various techniques discussed, as well as potential difficulties inherent to all steps in the biomarker discovery process. A vital resource for biochemists, biologists, analytical chemists, bioanalytical chemists, clinical and medical technicians, researchers in pharmaceuticals, and graduate students, Proteomic and Metabolomic Approaches to Biomarker Discovery provides the information needed to reduce clinical error in the execution of research. Describes the use of biomarkers to reduce clinical errors in research. Includes techniques from a range of biomarker discoveries. Covers all steps involved in biomarker discovery, from study design to study execution.
  • This book is designed to help scientists without a strong background in analytical chemistry to understand basic analytical principles so they can apply them to proteomics profiling. It will also help researchers with an analytical chemistry background to break into the proteomics field. The title focuses on practical applications for proteomic research so readers are guided to design better experiments and to more easily interpret the resulting data. Bridges the gap between overly specialized courses and books in mass spectrometry, proteomics and analytical chemistryCovers the analytical consequences of protein and peptide modifications that may have a profound effect on how and what researchers actually measure; other books do not discuss such issuesIncludes real life examples illustrating the importance of problems in quantitation and validation of biomarkers from the perspective of analytical chemistry with additional references to other sources Helps in designing and executing proteomic experiments with sound analytics.
  • Proteomic strategies for analyzing body fluids / Sung-Min Ahn and Richard J. Simpson -- Sample preparation of body fluids for proteomics analysis / Natalia Govorukhina and Rainer Bischoff -- Multiplexed immunoassays for protein profiling in human body fluids / Silke Wittemann ... [et al.] -- Deciphering the hieroglyphics of functional proteomics using small molecule probes / Wayne F. Patton -- Modification-specific proteomic analysis of glycoproteins in human body fluids by mass spectrometry / Jakob Bunkenborg, Per Hagglund, and Ole N. Jensen -- Plasma proteome database / Malabika Sarker, G. Hanumanthu, and Akhilesh Pandey -- 2D PAGE databases for proteins in human body fluids / Christine Hoogland ... [et al.] -- Bioinformatics and experimental design for biomarker discovery / Marc R. Wilkins and Sybille M.N. Hunt -- Integrative omics, pharmacoproteomics and human body fluids / K.K. Jain -- The human plasma and serum proteome / Gilbert S. Omenn ... [et al.] -- Proteomics of human urine / Visith Thongboonkerd ... [et al.] -- Proteomics of human cerebrospinal fluid / Margareta Ramström and Jonas Bergquist -- Proteomics of pleural effusion / Joost Hegmans, Annabrita Hemmes, and Bart Lambrecht -- Proteomics of bronchoalveolar lavage fluid and sputum / Ruddy Wattiez, Olivier Michel, and Paul Falmagne -- Proteomics of sinusitis nasal lavage fluid / Begona Casado, Simona Viglio, and James N. Baraniuk -- Proteomics of human saliva / Francisco M.L. Amado ... [et al.] -- Proteomics of human pancreatic juice / Mads Granborg, Anirban Maitra, and Akhilesh Pandey -- Proteomics of human bile / Troels Z. Kristiansen, Anirban Maitra, and Akhilesh Pandey -- Proteomics of amniotic fluid / David Crettaz ... [et al.] -- Proteomics of human milk / Amedeo Conti, Maria G. Giuffrida, and Maria Cavaletto -- Proteomics of nipple aspirate fluid in non-lactating women / Edward R. Sauter -- Proteomics of seminal fluid / Benjamin Solomon and Mark W. Duncan -- Proteomics of vitreous fluid / Atsushi Minamoto, Ken Yamane, and Tomoko Yokoyama -- Proteomics of human dialysate and ultrafiltrate fluids yielded by renal replacement therapy / Michael Walden ... [et al.].
  • Fulltext Springer Protocols
  • Overview of quantitative drug design -- Noncovalent interactions in biological systems -- Preparation of 3D structures of molecules for 3D QSAR -- Calculating physical properties of molecules -- Biological data -- Form of equations that relate potency and physical properties -- Statistical basis of regression and partial least-squares analysis -- Strategy for the statistical evaluation of a data set of related molecules -- Detailed examples of QSAR calculations on erythromycin esters -- Case studies -- Methods to approach other structure-activity problems.
  • 1. Fluorogenic derivatization followed by HPLC quantification and final identification of proteins by HPLC-tandem mass spectrometry (FD-LC-MS/MS) method / Akiyo Koshiyama, Tomoko Ichibangase, and Kazuhiro Imai -- 2. Two-dimensional difference gel electrophoresis / Viola Ruddat -- 3. Mass spectrometry utilizing isotope-coded affinity tag reagents / Naoyuki Yamada -- 4. Proteomic analyses of post-translational modifications / Wei-Chi Ku and Yasushi Ishihama -- 5. Cardiovascular proteomic analysis / Toru Suzuki and Ryozo Nagai -- 6. The proteome in neurodegenerative diseases / Teruyuki Tsuji, Masahiro Aoki, and Shun Shimohama -- 7. Liver disease-related proteomics / Hirofumi Uto, Yoichiro Takami, Shuji Kanmura, and Hirohito Tsubouchi -- 8. Respiratory disease-related proteome / Masahiro Seike and Akihiko Gemma -- 9. Renal disease-related proteome / Shinya Kaname and Tadashi Yamamoto -- 10. Aging-related proteome / Tosifusa Toda -- 11. Phosphoproteomics of tumor cell lines / Hisafumi Okabe -- 12. HCV infection and mitochondria proteomics / Kyoji Moriya, Takeya Tsutsumi, Hideyuki Miyoshi, and Kazuhiko Koike -- 13. Identification of biomarkers of infectious disease using surface-enhanced laser desorption/ionisation mass spectrometry / Shea Hamilton and Paul R. Langford -- 14. Quantitative proteomic analysis of HIV infection / Li Juan Fu and Sam F.Y. Li.
  • Acrylamide: a cysteine alkylating reagent for quantitative proteomics / Illarion V. Turko and Salvatore Sechi -- Using stable isotope tagging and mass spectrometry to characterize protein complexes and to detect changes in their composition / Jeffrey A. Ranish, Marjorie Brand, and Ruedi Aebersold -- Stable isotope labeling by amino acids in cell culture for quantitative proteomics / Shao-En Ong and Matthias Mann -- Quantitative proteomics of mouse brain and specific protein-interaction studies using stable isotope labeling / Toshitaka Sato, Yasushi Ishihama, and Yoshiya Oda -- The absolute quantification strategy: application to phosphorylation profiling of human separase serine 1126 / Scott A. Gerber ... [et al.] -- Quantification of proteins and metabolites by mass spectrometry without isotopic labeling / Sushmita Mimi Roy and Christopher H. Becker -- The use of a quantitative cysteinyl-peptide enrichment technology for high-throughput quantitative proteomics / Tao Liu ... [et al.] -- An isotope coding strategy for proteomics involving both amine and carboxyl group labeling / Fred E. Regnier -- Proteolytic labeling with ¹⁸O for comparative proteomics studies: preparation of ¹⁸O-labeled peptides and the ¹⁸O/¹⁶O peptide mixture / Catherine Fenselau and Xudong Yao -- Tandem mass spectrometry in the detection of inborn errors of metabolism for newborn screening / František Tureček, C. Ronald Scott, and Michael H. Gelb -- Absolute quantification of specific proteins in complex mixtures using visible isotope-coded affinity tags / Yu Lu ... [et al.] -- Computational analysis of quantitative proteomics data using stable isotope labeling / Michael J. MacCoss and Christine C. Wu -- Quantitative proteomic analysis of mammalian organisms using metabolically labeled tissues / Christine C. Wu, and Michael J. MacCoss -- Quantitative proteomic analysis of phosphotyrosine-mediated cellular signaling networks / Yi Zhang, Alejandro Wolf-Yadlin, and Forest M. White.
  • Quinones and quinone enzymes. Part A-B pt. A-B, 2004., ScienceDirect
    pt. B ScienceDirect
  • RAS family GTPases 2006, Springer
  • pt. B ScienceDirect
  • From Neanderthal to nanobiotech : from plant potions to pharming with plant factories / Christophe Sourrouille ... [et al.] -- Cowpea mosaic virus-based systems for the expression of antigens and antibodies in plants / Frank Sainsbury, Li Liu, and George P. Lomonossoff -- Transient expression of antibodies in plants using syringe agroinfiltration / Marc-André D'Aoust ... [et al.] -- Rapid system for evaluating bioproduction capacity of complex pharmaceutical proteins in plants / Giuliana Medrano ... [et al.] -- Production and localization of recombinant pharmaceuticals in transgenic seeds / Thomas Rademacher, Elsa Arcalis, and Eva Stoger -- Production of antibody fragments in Arabidopsis seeds / Bart Van Droogenbroeck, Kirsten De Wilde, and Ann Depicker -- Production of plantibodies in Nicotiana plants / Marta Ayala ... [et al.] -- Physcomitrella patens : a non-vascular plant for recombinant protein production / David Liénard and Fabien Nogué -- Production of recombinant proteins in suspension-cultured plant cells / Carole Plasson ... [et al.] -- Chloroplast-derived vaccine antigens and biopharmaceuticals : protocols for expression, purification, or oral delivery and functional evaluation / N. Dolendro Singh, Yi Ding, and Henry Daniell -- Protein body induction : a new tool to produce and recover recombinant proteins in plants / Margarita Torrent, Imma Llop-Tous, and M. Dolors Ludevid -- A case study for plant-made pharmaceuticals comparing different plant expression and production systems / Guy Vancanneyt ... [et al.] -- Glycosylation of antibody therapeutics : optimisation for purpose / Roy Jefferis -- N-glycosylation of plant recombinant pharmaceuticals / Muriel Bardor ... [et al.] -- Companion protease inhibitors to protect recombinant proteins in transgenic plant extracts / Meriem Benchabane ... [et al.] -- Strategies for improving vaccine antigens expression in transgenic plants : fusion to carrier sequences / Jose M. Escribano and Daniel M. Perez-Filgueira -- Immunomodulation of plant function by in vitro selected single-chain Fv intrabodies / Manfred Gahrtz and Udo Conrad -- On-chip detection of low-molecular-weight recombinant proteins in plant crude extracts by SELDI-TOF MS / Amine M. Badri ... [et al.] -- Assessing the risk of undesirable immunogenicity/allergenicity of plant-derived therapeutic proteins / Paul D. Chamberlain -- Biosafety, risk assessment, and regulation of plant-made pharmaceuticals / Penelope A.C. Sparrow and Richard M. Twyman.
  • Actin filament dynamics using microfluidics -- Bacterial actin-like proteins: purification and characterization of self-assembly properties -- Quantitative analysis of microtubule self-assembly kinetics and tip structure -- Biochemical reconstitution of the WAVE regulatory complex -- Rotational movement of formins evaluated by using single-molecule fluorescence polarization -- Single-molecule studies of actin assembly and disassembly factors -- Assaying microtubule nucleation of the [gamma]-Tubulin ring complex -- Reconstituting dynamic microtubule polymerization regulation by TOG domain proteins -- Generation of differentially modified microtubules using In Vitro enzymatic approaches -- Engineering defined motor ensembles with DNA origami -- Construction and analyses of elastically coupled multiple-motor systems -- Reconstitution of cortical dynein function -- Reconstitution of microtubule-dependent organnelle transport -- Reconstituting the motility of isolated intracellular cargoes -- Reconstitution of contractile actomyosin arrays -- Directed actin assembly and motility -- In Vitro reconstitution of dynamic microtubules interacting with actin filament networks -- Measuring kinetochore-microtubule interaction In Vitro -- Micropattern-guided assembly of overlapping pairs of dynamic microtubules -- WAVE regulatory complex activation -- Dissecting principles governing actin assembly using yeast extracts -- Xenopus egg ctyoplasm with intact actin -- Glycogen-supplemented mitotic cytosol for analyzing xenopus egg microtubule organization -- Spindle assembly on immmobilized chromatin micropatterns.
  • The role of redox in signal transduction / John T. Hancock -- The measurement of nitric oxide and its metabolites in biological samples by ozone-based chemiluminescence / Andrew G. Pinder ... [et al.] -- Detection and measurement of reactive oxygen intermediates in mitocondria and cells / Matthew Whiteman ... [et al.] -- Redox-sensitive green fluorescent protein : probes for dynamic intracellular redox responses : a review / Mark B. Cannon and S. James Remington -- Measuring redox changes in vivo in leaves : prospects and technical challenges / Philip M. Mullineaux and Tracy Lawson -- Imaging of intracellular hydrogen peroxide production with hyper upon stimulation of hela cells with Egf / Kseniya N. Markvicheva ... [et al.] -- Tools to investigate ROS sensitive signalling proteins / Radhika Desikan ... [et al.] -- Methods for preparing crystals of reversibly oxidized proteins : crystallization of protein tyrosine phophatase 1b as an example / Annette Salmeen and David Barford -- Methods for the study of redox-mediated changes in p53 structure and function / Kristine Mann -- Redox regulation and trapping sulphenic acid in the peroxide sensitive human mitochondrial branched chain aminotransferase / Susan M. Hutson ... [et al.] -- Detection of carbonylated proteins in 2-D SDS page separations / Rukhsana Sultana ... [et al.] -- Analysis of global and specific changes in the disulfide proteome using redox 2D-PAGE / Robert C. Cumming -- Protein-thiol oxidation from single proteins to proteome-wide analyses / Natacha Le Moan, Frédérique Tacnet, and Michel B. Toledano -- Analysis of redox relationships in the plant cell cycle : determinations of ascorbate, glutathione, and poly (ADPribose) polymerase (PARP) in plant cell cultures / Christine H. Foyer ... [et al.] -- Generation and detection of S-nitrosothiols / Christian Lindermayr, Simone Sell, and Jörg Durner.
  • Examining the molecular mechanism of Bcl-2 family proteins at membranes by fluorescence spectroscopy -- Photoreactive stapled peptides to identify and characterize BCL-2 family interaction sites by mass spectrometry -- The structural biology of BH3-only proteins -- How to analyze mitochondrial morphology in healthy cells and apoptotic cells in Caenorhabditis elegans -- Apoptosis initiation through the cell-extrinsic pathway -- Using RNAi screening technologies to interrogate the extrinsic apoptosis pathway -- Caspase enzymology and activation mechanisms -- Turning on caspases with genetics and small molecules -- A multipronged approach for compiling a global map of allosteric regulation in the apoptotic caspases -- Measuring caspase activity in vivo -- Single-molecule sensing of caspase activation in live cells via plasmon coupling nanotechnology -- In vivo monitoring of caspase activation using a fluorescence resonance energy transfer-based fluorescent probe -- Global analysis of cullular proteolysis by selective enzymatic labelling of protein N-termini -- Complementary methods for the indentification of substrates of proteolysis -- Phospholipid scrambling on the plasma membrane -- Studying apoptosis in the zebrafish.
  • AAA+ proteolytic machines -- Machines of destruction: AAA+ proteases and the adaptors that control them / Eyal Gur, Ralf Ottofueling, David A. Dougan -- The Lon AAA+ protease / Eyal Gur -- FtsH protease-mediated regulation of various cellular functions / Takashi Okuno, Teru Ogura -- Regulatory proteolysis in bacteria -- General and regulatory proteolysis in Bacillus subtilis / Nöel Molière, Kürşad Turgay -- Proteolytic regulation of stress response pathways in Escherichia coli / Dimce Micevski, David A. Dougan -- Regulated proteolysis: control of the Escherichia coli [sigma]E-dependent cell envelope stress response / Sarah E. Barchinger, Sarah E. Ades -- Bacterial proteases and virulence / Dorte Frees, Lone Brøndsted, Hanne Ingmer -- Regulated proteolysis in yeast -- Roles of Cdc48 in regulated protein degradation in yeast / Alexander Buchberger -- The Role of AAA+ proteases in mitochondrial protein biogenesis, homeostasis and activity control / Wolfgang Voos, Linda A. Ward, Kaye N. Truscott -- Ubiquitin-like protein modification and protein degradation in microorganisms -- The pup-proteasome system of mycobacterium tuberculosis / Marie I. Samanovic, Huilin Li, K. Heran Darwin -- Archaeal proteasomes and sampylation / Julie A. Maupin-Furlow.
  • Regulators of G-protein signaling. Part A-B pt. A-B, 2004., ScienceDirect
    pt. B ScienceDirect
  • This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in biomineralization science, and includes sections on such topics as determining solution chemistry, structure and nucleation; probing structure and dynamics at surfaces; and interfaces mapping biomineral and morphology and ultrastructure.
  • pt. B ScienceDirect
  • RHO family GTPases 2005, Springer
  • Rho GTPases in cancer 2010, Springer
  • Ribonuclease P 2010, Springer
    History of RNase P and Overview of Its Catalytic Activity -- The Evolution of RNase P and Its RNA -- Over a Decade of Bacterial Ribonuclease P Modeling -- Structural Studies of Ribonuclease P -- Folding of Bacterial RNase P RNA -- Kinetic Mechanism of Bacterial RNase P -- Roles of Metal Ions in RNase P Catalysis -- Challenges in RNase P Substrate Recognition: Considering the Biological Context -- Archaeal RNase P: A Mosaic of Its Bacterial and Eukaryal Relatives -- Eukaryote RNase P and RNase MRP -- RNase P from Organelles -- Human RNase P and Transcription -- RNase P as a Drug Target -- Ribonuclease P as a Tool.
  • Ribonucleases 2011, Springer
  • This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch structure and function methods, folding pathways and dynamics, and ligand interactions and thermodynamics.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in biomineralization scienceContains sections on such topics as riboswitch discovery and valid.
  • Introduction / Michael Levitt -- Modeling RNA Molecules / Neocles Leontis and Eric Westhof -- Methods for Predicting RNA Secondary Structure / Kornelia Aigner, Fabian Dre€en and Gerhard Steger -- Why Can't We Predict RNA Structure At Atomic Resolution? / Parin Sripakdeevong, Kyle Beauchamp and Rhiju Das -- Template-Based and Template-Free Modeling of RNA 3D Structure: Inspirations from Protein Structure Modeling / Kristian Rother, Magdalena Rother, Michał Boniecki, Tomasz Puton and Konrad Tomala, et al. -- The RNA Folding Problems: Different Levels of sRNA Structure Prediction / Fredrick Sijenyi, Pirro Saro, Zheng Ouyang, Kelly Damm-Ganamet and Marcus Wood, et al. -- Computational Prediction and Modeling Aid in the Discovery of a Conformational Switch Controlling Replication and Translation in a Plus-Strand RNA Virus / Wojciech K. Kasprzak and Bruce A. Shapiro -- Methods for Building and Refining 3D Models of RNA / Samuel C. Flores, Magdalena Jonikas, Christopher Bruns, Joy P. Ku and Jeanette Schmidt, et al. -- Multiscale Modeling of RNA Structure and Dynamics / Feng Ding and Nikolay V. Dokholyan -- Statistical Mechanical Modeling of RNA Folding: From Free Energy Landscape to Tertiary Structural Prediction / Song Cao and Shi-Jie Chen -- Simulating Dynamics in RNA-Protein Complexes / John Eargle and Zaida Luthey-Schulten -- Quantum Chemical Studies of Recurrent Interactions in RNA 3D Motifs / Jiří Šponer, Judit E. Šponer and Neocles B. Leontis -- Nonredundant 3D Structure Datasets for RNA Knowledge Extraction and Benchmarking / Neocles B. Leontis and Craig L. Zirbel -- Ions in Molecular Dynamics Simulations of RNA Systems / Pascal Auffinger -- Modeling RNA Folding Pathways and Intermediates Using Time-Resolved Hydroxyl Radical Footprinting Data / Joshua S. Martin, Paul Mitiguy and Alain Laederach -- A Top-Down Approach to Determining Global RNA Structures in Solution Using NMR and Small-Angle X-ray Scattering Measurements / Yun-Xing Wang, Jinbu Wang and Xiaobing Zuo -- RNA Structure Determination by Structural Probing and Mass Spectrometry: MS3D / A. E. Hawkins and D. Fabris.
  • RNA and cancer 2013, Springer
    Coupling Between Transcription and Alternative Splicing / Ignacio E. Schor, Luciana I. Gómez Acuña, Alberto R. Kornblihtt -- Detection of Alternatively Spliced or Processed RNAs in Cancer Using Oligonucleotide Microarray / Marieta Gencheva ... [et al.] -- Cancer-Associated Perturbations in Alternative Pre-messenger RNA Splicing / Lulzim Shkreta ... [et al.] -- Alternative Splicing of Tumor Suppressors and Oncogenes / Claudia Ghigna, Silvano Riva, Giuseppe Biamonti -- MicroRNAs in Cancer / Jianzhong Jeff Xi -- The Perinucleolar Compartment: RNA Metabolism and Cancer / John T. Norton, Sui Huang -- Regulation of ARE-mRNA Stability by Cellular Signaling: Implications for Human Cancer / Christian Kroun Damgaard, Jens Lykke-Andersen -- Alternative Pre-mRNA Splicing, Cell Death, and Cancer / Kong Ruirui ... [et al.] -- Oligonucleotide Therapeutics in Cancer / Jing Wan, John A. Bauman, Maria Anna Graziewicz, Peter Sazani -- Clinical Perspective on Chemo-Resistance and the Role of RNA Processing / Nancy L. Krett, Shuo Ma, Steven T. Rosen.
  • RNA exosome 2011, Springer
    "The diversity of RNAs inside living cells is amazing. We have known of the more "classic" RNA species: mRNA, tRNA, rRNA, snRNA and snoRNA for some time now, but in a steady stream new types of molecules are being described as it is becoming clear that most of the genomic information of cells ends up in RNA. To deal with the enormous load of resulting RNA processing and degradation reactions, cells need adequate and efficient molecular machines. The RNA exosome is arising as a major facilitator to this effect. Structural and functional data gathered over the last decade have illustrated the biochemical importance of this multimeric complex and its many co-factors, revealing its enormous regulatory power. By gathering some of the most prominent researchers in the exosome field, it is the aim of the book to introduce this fascinating protein complex and to give a timely and rich account of its many functions"--Provided by publisher.
  • RNA helicases. 1st ed. 2012, ScienceDirect
    This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases. This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases The contributions in this volume cover the broad scope of methods in the research on these enzymes.
  • RNA interference 2008, Springer
    RNA interference in mammalian cell systems / Patrick J. Paddison -- RNAi pathway in C. elegans: the argonautes and collaborators / Marie-Eve L. Boisvert and Martin J. Simard -- Genetics and biochemistry of RNAi in drosophila / Harsh H. Kavi ... [et al.] -- Role of dicer in posttranscriptional RNA silencing / Lukasz Jaskiewicz and Witold Filipowicz -- The mechanism of RNase III action: how dicer dices / Xinhua Ji -- MicroRNA metabolism in plants / Xuemei Chen -- Structure-function relationships among RNA-dependent RNA polymerases / Kenneth K.-S. Ng, Jamie J. Arnold, and Craig E. Cameron -- RNAi-mediated chromatin silencing in fission yeast / Sharon A. White and Robin C. Allshire -- A role for RNAi in heterochromatin formation in drosophila / Nicole C. Riddle and Sarah C.R. Elgin -- RNA mediated transcriptional gene silencing in human cells / Kevin V. Morris -- RNA silencing in mammalian oocytes and early embryos / Petr Svoboda -- Identifying human microRNAs / Isaac Bentwich.
  • 1. RNA polymerases and transcription factors of trypanosomes / Arthur Günzl -- 2. SL RNA biogenesis in kinetoplastids: a long and winding road / Nancy R. Sturm, Jesse R. Zamudio, and David A. Campbell -- 3. Pre-mRNA splicing in trypanosoma brucei: factors, mechanisms, and regulation / Christian Preusser, Nicolas Jaé, Arthur Günzl, and Albrecht Bindereif -- 4. mRNA turnover in trypanosomes / Christine Clayton -- 5. tRNA biogenesis and processing / Jessica L. Spears, Mary Anne T. Rubio, Paul J. Sample, and Juan D. Alfonzo -- 6. rRNA biogensis in trypanosomes / Shulamit Michaeli -- 7. RNA editing in African trypanosomes: a U-ser's G-U-ide / H. Ulrich Göringer -- 8. The RNA interference pathway in trypanosoma brucei / Elisabetta Ullu, Nikolay G. Kolev, Rebecca L. Barnes, and Christian Tschudi -- 9. Translation in trypanosomatids -- 10. Mitochondrial translation in trypanosomatids / Dmitri A. Maslov and Rajendra K. Agrawal -- 11. RNA-Seq analysis of the transcriptome of trypanosoma brucei / Jan Mani, Kapila Gunasekera, and Isabel Roditi.
  • RNA and the cellular biochemistry revisited -- RNA isolation strategies -- The truth about tissues -- Going green : RNA and the molecular biology of plants -- Isolation of polyadenylated RNA -- Quality control for RNA preparations -- Resilient ribonucleases -- Stringency : conditions that influence nucleic acid structure -- Electrophoresis of RNA -- Photodocumentation and image analysis -- Northern analysis -- Nucleic acid probe technology -- Practical nucleic acid hybridization -- Principles of detection -- Quantifications of specific mRNAs by nuclease protection -- Analysis of nuclear RNA -- cDNA synthesis -- RT-PCR : a science and an art form -- Quantitative PCR techniques -- Functional genomics and transcript profiling -- High-throughput analysis of gene expression -- Non-array methods for global analysis of gene expression -- RNAi : take a RISC-role the dicer -- Genomes, transcriptomes, proteomes, and bioinformatics -- An RNA paradigm.
  • pt. D ScienceDirect
  • RNA towards medicine 2006, Springer
  • It is now well known that proteases are found everywhere, in viruses and bacteria as well as in all human, animal and plant cells, and play a role in a variety of biological functions ranging from digestion, fertilization, development to senescence and death. Under physiological conditions the ability of proteases is regulated by endogenous inhibitors. However, when the activity of proteases is not regulated appropriately, disease processes can result, as seen in Alzheimer's disease, cancer metastasis and tumor progression, inflammation and atherosclerosis. Thus it is evident that there is an absolute need for a tighter control of proteolytic activities in different cells and tissues. Aimed at graduate students and researchers with an interest in cellular proteolytic events, Role of Proteases in Cellular Dysfunctions is the second book on Proteases in this series. The book consists of three parts in specified topics based on current literatures for a better understanding for the readers with respect to their subject-wise interests. The first section of this book covers a brief idea about the neuronal disorders and the involvement of proteases such as calpains, caspases and matrix metalloproteases (MMPs). The second section covers the deadly disease cancer and its relation to ubiquitin-proteosomal system, MMPs and serine proteases. The last section is about the role of proteases such as calpains, MMPs and serine protease as well as urokinase type plasminogen activator receptor (uPAR) in causing cardiovascular defects.
  • Pt. 1. Selenocysteine biosynthesis and incorporation into protein -- pt. 2. Selenoproteins and selenoproteins in health -- pt. 3. Selenium and selenoproteins in human health -- pt. 4. Mouse models for elucidating the role of selenium and selenoproteins in health.
  • Separation science and technology v. 1-, 1998-, ScienceDirect
  • Intracellular production of recombinant serpins in yeast / Dion Kaiserman ... [et al.] -- Production of recombinant serpins in Escherichia coli / Mary C. Pearce and Lisa D. Cabrita -- Isolation and characterization of the nuclear serpin MENT / Sergei Grigoryev and Sheena McGowen -- Solving serpin crystal structures / Randy J. Read, Aiwu Zhou, and Penelope E. Stein -- Crystallography of serpins and serpin complexes / M.A. Dunstone and James Whisstock -- Serpins as hormone carriers : modulation of release / Robin Carrell, Xiaoqiang Qi, and Aiwu Zhou -- Serpin-glycosaminoglycan interactions / Chantelle M. Rein, Umesh R. Desai, and Frank C. Church -- Targeting serpins in high-throughput and structure-based drug design / Yi-Ping Chang ... [et al.] -- Development of inhibitors of plasminogen activator inhibitor-1 / Shih-Hon Li and Daniel A. Lawrence -- Bioinformatic approaches for the identification of serpin genes with multiple reactive site loop coding exons / Stefan Börner and Hermann Ragg -- Methods to measure the kinetics of protease inhibition by serpins / Anita J. Horvath ... [et al.] -- Predicting serpin/protease interactions / Jiangning Song ... [et al.] -- Amino-Terminal Oriented Mass spectrometry of Substrates (ATOMS) : N-terminal sequencing of proteins and proteolytic cleavage sites by quantitative mass spectrometry / Alain Doucet and Christopher M. Overall -- Computational methods for studying serpin conformational change and structural plasticity / Itamar Kass, Cyril F. Reboul, and Ashley M. Buckle -- Probing serpin conformational change using mass spectrometry and related methods / Yuko Tsutsui, Anindya Sarkar, and Patrick L. Wintrode -- Determining serpin conformational distributions with single molecule fluorescence / Nicole Mushero and Anne Gershenson -- Serpin polymerization in vitro / James A. Huntington and Masayuki Yamasaki -- The serpinopathies : studying serpin polymerization in vivo / James A. Irving ... [et al.].
  • Assessing the response of cells to TLR stimulation / Kiyoshi Takeda, Masahiro Yamamoto, and Kenya Honda -- TLR ligands in experimental settings their purity and specificity / Min-Fu Tsan -- Analysis of TLR expression, regulation, and signaling / Hui Xiao, Xiaoxia Li, and Derek W. Abbott -- Intracellular trafficking of toll-like receptors / Harald Husebye ... [et al.] -- The role of small RhoGTPases in TLR signaling / Monica Ruse and Ulla G. Knaus -- Use of engineered [beta]-lactamase fragment complementation to detect the associations of toll-like receptors and signaling adaptors / Hyun-Ku Lee and Peter S. Tobias -- Use of toll-like receptor chimeras to dissect mechanisms of receptor localization and signaling / Tadashi Nishiya and Anthony L. DeFranco -- Engagement of toll-like receptors modulates chemokine receptor signaling / Chong-Shan Shi and John H. Kehrl -- Role of toll-like receptor signaling in central nervous system infections / Tammy Kielian -- The influence of injury on toll-like receptor responses / Thomas J. Murphy ... [et al.].
  • Signaling in the heart 2011, Springer
    Tools to Study Signaling -- Cell-Cycle Signaling, Epigenetics, and Nuclear Function -- Signaling in the Endothelium -- Rapid Signaling Pathways -- Growth Factors Signaling -- Ion Signaling and Electrophysiological Function -- Lipid Signaling Pathways in the Heart -- Heart Mitochondria: A Receiver and Integrator of Signals -- Signaling Pathways in Cardiovascular Development -- Signaling in Congenital Heart Disease -- Signaling in the Aging Heart -- Signaling in Endomyocarditis -- Signaling in Hypertension -- Gene Expression and Signaling Pathways in Myocardial Ischemia -- Signaling in Hypertrophy and Heart Failure -- Signaling in Diabetes and Metabolic Syndrome -- Dysrhythmias/Channelopathies and Signaling Pathways -- Signaling in Atherosclerosis -- Stem Cells Signaling Pathways in the Heart -- Cardioprotection and Signaling Pathways -- Targeting Signaling Pathways -- Signaling and the Frontiers Ahead.
  • Single-molecule measurement techniques are providing fundamental information on the structure and function of biomolecules and are becoming an indispensable tool to understand how proteins work. During the last two decades, this field has grown at an almost exponential rate in terms of biological and biophysical applications. Single-molecule techniques have opened new fields of science that are at the crossroads of several disciplines such as biology, physics, chemistry, material science and computer science. These methods are often the approach of choice to clarify and better understand the structure and function of single proteins. This volume consists of up-to-date and comprehensive reviews of important and timely applications of different biological problems tackled by single-molecule methods; it also covers basic principles of operation, experiment and theory.In Single-molecule Studies of Proteins, expert researchers discuss the successful application of single-molecule techniques to a wide range of biological events, such as the imaging and mapping of cell surface receptors, the analysis of the unfolding and folding pathways of single proteins, the analysis interaction forces between biomolecules, the study of enzyme catalysis or the visualization of molecular motors in action. The chapters are aimed at established investigators and post-doctoral researchers in the life sciences wanting to pursue research in the various areas in which single-molecule approaches are important; this volume also remains accessible to advanced graduate students seeking similar research goals.
  • Small GTPases in disease. Part A-B pt. A-B, 2008., ScienceDirect
    pt. B ScienceDirect
  • Hydrogen-bonded synthetic mimics of protein secondary structure as disruptors of protein-protein interactions / M.J. Adler, A.G. Jamieson and Andrew D. Hamilton -- Small-molecule inhibitors of IL-2/IL-2R : Lessons learned and applied / C.G. Wilson and M.R. Arkin -- Small molecule inhibitors of the human papillomavirus E1-E2 interaction / P.W. White, Anne-Marie Faucher and Nathalie Goudreau -- Design of small-molecule Smac mimetics as IAP antagonists / Shaomeng Wang -- Small-molecule inhibitors reveal a new function for Bcl-2 as a proangiogenic signaling molecule / Benjamin D. Zeitlin and Jacques E. Nör -- Small-molecule modulators of c-Myc/Max and Max/Max interactions / Thorsten Berg -- Small-molecule inhibitors of the p53-MDM2 interaction / Binh T. Vu and Lyubomir Vassilev.
  • The systems-based approach to proteomics and chemical proteomics / Daniel S. Sem -- Similarities in protein binding sites / Hugo O. Villar, Mark R. Hansen, and Richard Kho -- Survey of spectral techniques used to study proteins / Daniel S. Sem -- Capillary electrophoresis, mass spectrometry for characterization of peptides and proteins / Christian Neusüss and Matthias Pelzing -- Protein and peptide analysis by matrix-assisted laser desorption/ionization tandem mass spectrometry (Maldi MS/MS) / Emmanuelle Sachon and Ole Nørregaard Jensen -- Characterization of glycosylated proteins by mass spectrometry using microcolumns and enzymatic digestion / Per Hägglund and Martin R. Larsen -- Surface-enhanced laser desorption/ionization protein biochip technology for proteomics research and assay development / Scot R. Weinberger ... [et al.] -- An approach to the reproducibility of SELDI profiling / Walter S. Liggett ... [et al.] -- Mass spectrometric applications in immunoproteomics / Anthony W. Purcell ... [et al.] -- Near-infrared fluorescence detection of antigen-antibody interactions on microarrays / Sakanyan Vehary and Yeretssian Garabet -- Application of shotgun proteomics to transcriptional regulatory pathways / Amber L. Mosley and Michael P. Washburn -- Electrophoretic NMR of protein mixtures and its proteomic applications / Qiuhong He, Sunitha B. Thakur, and Jeremy Spater -- Characterizing proteins and proteomes using isotope-coded mass spectrometry / Uma Kota and Michael B. Goshe -- Surface plasmon resonance biosensors’ contributions to proteome mapping / Rebecca L. Rich and David G. Myszka -- Application of in-cell NMR spectrocopy to investigation of protein behavior and ligand-protein interaction inside living cells / Volker Dötsch -- An overview of metabonomics techniques and applications / John C. Lindon, Elaine Holmes, and Jeremy K. Nicholson -- NMR-based structural proteomics / John L. Markley -- Leveraging x-ray structural information in gene family-based drug discovery: application to protein kinases / Marc Jacobs ... [et al.] -- EPR spectroscopy in genome-wide expression studies / Richard Cammack -- Summary of chapters and future prospects for spectral techniques in proteomics / Daniel S. Sem.
  • v. 2, 2006 Springer
    v. 3, 2006 Springer
    v. 4, 2006 Springer
    v. 5, 2006 Springer
    v. 6, 2006 Springer
    v. 1. Cholestanes, ergostanes, withanolides, stigmastane -- v. 2. Stigmastanes, furostanes, spirtostanes-- v. 3. Spirostanes, bufanolides, cardenolides-- v. 4. Cardenolides and pregnanes -- v. 5. Pregnanes, androstanes, and miscellaneous -- v. 6. Miscellaneous steroids and indexes.
  • Sphingolipid biology 2006, Springer
  • An overview of sphingolipid metabolism : from synthesis to breakdown / Christopher R. Gault, Lina M. Obeid, and Yusuf A. Hannun -- Sphingolipid transport / Laura Riboni, Paola Giussani and Paola Viani -- Sphingolipid analysis by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) / Jacek Bielawski ... [et al.] -- Ceramide synthases : roles in cell physiology and signaling / Johnny Stiban, Rotem Tidhar and Anthony H. Futerman -- Tales and mysteries of the enigmatic sphingomyelin synthase family / Joost C.M. Holthuis and Chiara Luberto -- Ceramide in stress response / Mariana N. Nikolova-Karakashian and Krassimira A. Rozenova -- Animal models for studying the pathophysiology of ceramide / Toshihiko Kawamori -- Ceramide 1-phosphate in cell survival and inflammatory signaling / Antonio Gómez-Muñoz ... [et al.] -- Ceramide-1-phosphate in phagocytosis and calcium homeostasis / Vania Hinkovska-Galcheva and James A. Shayman -- Extracellular and intracellular actions of sphingosine-1-phosphate / Graham M. Strub ... [et al.] -- Glucosylceramide in humans / Maria C. Messner and Myles C. Cabot -- Gangliosides as regulators of cell membrane organization and functions / Sandro Sonnino and Alessandro Prinetti -- Cancer treatment strategies targeting sphingolipid metabolism / Babak Oskouian and Julie D. Saba -- Therapeutic strategies for diabetes and complications : a role for sphingolipids? / Todd E. Fox and Mark Kester -- Roles for sphingolipids in Saccharomyces cerevisiae / Robert C. Dickson -- Sphingolipid signaling in fungal pathogens / Ryan Rhome and Maurizio Del Poeta -- Sphingolipids in parasitic protozoa / Kai Zhang, James D. Bangs and Stephen M. Beverley -- Biosynthesis of sphingolipids in plants (and some of their functions) / Simone Zäuner, Philipp Ternes, and Dirk Warnecke -- Computational analysis of sphingolipid pathway systems / Eberhard O. Voit, Fernando Alvarez-Vasquez, and Yusuf A. Hannun.
  • Sphingolipids are lipid components of the plasma membrane in eukaryotic cells. They have an important function in signaling mechanisms in the cell. This book on sphingolipids provides insights into the basics of sphingolipid biology and drug development, with a particular emphasis on the sphingolipid derivative ceramide. In the first part basic functions of sphingolipids are described, as well as the genetics of important enzymes, sphingolipid metabolism and synthesis. The second part of this first volume focuses on drug development and pharmacology. The book is intended for scientists in pharmacology, biochemistry and cell biology with a focus on biomedical research as well as for clinicians working in pharmacology, oncology, cardiology, neurology and infectious disease. Together with Volume 216 by the same editors, the collection represents a unique, comprehensive work on sphingolipids, providing information on both sphingolipids' basic biology (including synthesis, metabolism and cell biology) and their important function in a (patho-)physiological context.
  • Sphingolipids in disease 2013, Springer
    Sphingolipids in Cancer. Sphingosine Kinase/Sphingosine 1-Phosphate Signaling in Cancer Therapeutics and Drug Resistance / Shanmugam Panneer Selvam, Besim Ogretmen -- Using ASMase Knockout Mice to Model Human Diseases / Guoqiang Hua, Richard Kolesnick -- New Perspectives on the Role of Sphingosine 1-Phosphate in Cancer / Susan Pyne, Nigel J. Pyne -- Sphingolipids and Response to Chemotherapy / Marie-Thérèse Dimanche-Boitrel, Amélie Rebillard -- Lung Cancer and Lung Injury: The Dual Role of Ceramide / Tzipora Goldkorn, Samuel Chung, Simone Filosto -- Sphingolipids' Role in Radiotherapy for Prostate Cancer / Carla Hajj, Adriana Haimovitz-Friedman -- Sphingolipids in Cardio-Reno-vascular Diseases. Sphingolipid Metabolism and Atherosclerosis / Xian-Cheng Jiang, Jing Liu -- Cardiovascular Effects of Sphingosine-1-Phosphate (S1P) / Bodo Levkau -- Cross Talk Between Ceramide and Redox Signaling: Implications for Endothelial Dysfunction and Renal Disease / Pin-Lan Li, Yang Zhang -- Sphingolipids in Inflammation, Infection and Lung Diseases. Sphingolipids in Lung Endothelial Biology and Regulation of Vascular Integrity / Taimur Abbasi, Joe G. N. Garcia -- Sphingolipids in Acute Lung Injury / Stefan Uhlig, Yang Yang -- The Involvement of Sphingolipids in Chronic Obstructive Pulmonary Diseases / Irina Petrache, Daniela N. Petrusca -- Ceramide in Cystic Fibrosis / Heike Grassmé, Joachim Riethmüller, Erich Gulbins -- Regulation of the Sphingosine Kinase/Sphingosine 1-Phosphate Pathway / K. Alexa Orr Gandy, Lina M. Obeid -- Bacterial Infections and Ceramide / Heike Grassmé, Katrin Anne Becker -- Viral Infections and Sphingolipids / Jürgen Schneider-Schaulies, Sibylle Schneider-Schaulies -- Ceramide in Plasma Membrane Repair / Annette Draeger, Eduard B. Babiychuk -- Sphingolipids and Inflammatory Diseases of the Skin / Burkhard Kleuser, Lukasz Japtok -- Sphingolipids in Obesity, Type 2 Diabetes, and Metabolic Disease / S. B. Russo, J. S. Ross, L. A. Cowart -- Sphingolipids in Neuro-psychiatry and Muscle Diseases. Neuronal Forms of Gaucher Disease / Einat B. Vitner, Anthony H. Futerman Sphingolipids in Neuroinflammation / Laura Davies, Klaus Fassbender, Silke Walter -- Sphingolipids in Psychiatric Disorders and Pain Syndromes / C. Mühle, M. Reichel, E. Gulbins, J. Kornhuber -- Role of Sphingosine 1-Phosphate in Skeletal Muscle Cell Biology / Paola Bruni, Chiara Donati.
  • v. 1. Class 5: Isomerases -- v. 2. Class 6: Ligases -- v. 3. Class 4: Lyases I -- v. 4. Class 4: Lyases II -- v. 5. Class 4: Layases III -- v. 6. Class 3.4: Hydrolases I -- v. 7. Class 3.4: Hydrolases II -- v. 8. Class 3.4: Hydrolases III -- Index A: Synonym -- v. 9. Class 3.1: Hydrolases IV -- v. 10. Class 3.1: Hydrolases V -- v. 11. Class 3.1: Hydroclass VI -- v. 12. Class 3.2: Hydrolases VII -- v. 13. Class 3.2: Hydrolases VIII -- v. 14. Class 3.2-3.5: Hydrolases IX -- v. 15. Class 3.5-3.12: Hydrolases X -- v. 16. Class 1: Oxidoreductases I -- v. 17. Class 1: Oxidoreductases II -- v. 18. Class 1: Oxidoreductases III -- v. 19. Class 1: Oxidoreductases IV -- v. 20. Class 1: Oxidoreductases V. Class 1: Oxidoreductases VI -- v. 22. Class 1: Oxidoreductases VII -- v. 23. Class 1: Oxidoreductases VIII. -- v. 24. Class 1: Oxidoreductases IX -- v. 25. Class 1: Oxidoreductases X -- v. 26. Class 1: Oxidoreductases XI -- v. 27. Class 1: Oxidoreductases XII -- v. 28. Class 2: Transferases I -- v. 29. Class 2: Transferases II -- v. 30. Class 2: Transferases III -- v. 31. Class 2: Transferases IV -- v. 32. Class 2: Transferases V -- v. 33. Class 2: Transferases VI EC -- v. 34. Class 2: Transferases VI EC -- v. 35. Class 2: Transferases VIII -- v. 36. Class 2: Transferases IX -- v. 37. Class 2: Transferases X -- v. 38. Class 2: Transferases XI -- v. 39. Class 2: Transferases XII -- v. S1. Class 1: Oxidoreductases EC 1 -- v. S2. Class 2: Transferases EC 2.1-2.7.10 -- v. S3. Class 2: Transferases EC -- v. S4. Class 2: Transferases EC -- v. S5. Class 3: Hydrolases EC 3.1-3.4.21 -- v. S6. Class 3: Hydrolases EC3.4.22-3.13 -- v. S7. Class 4-6: Lyases, Isomerases, Ligases EC 4-6.
  • Obesity and diabetes develop as a complex result of genetic, metabolic and environmental factors and are characterized by increased lipogenesis and lipid accumulation in many tissues. Stearoyl-CoA desaturase (SCD) genes are a critical regulator of lipogenesis and catalyzes the synthesis of monounsaturated fatty acids (MUFA), mainly oleoyl- (18:1n9) and palmitoleoyl-CoA (16:1n7). These MUFAs are the major fatty acid substrates for the synthesis of triglycerides, cholesterol esters, wax esters and membrane phospholipids. There are 4 SCD isoforms (SCD1-4) in mice and two (hSCD1 and hSCD5) expressed in humans. At first glance, stearoyl-CoA desaturase enzyme would be considered a housekeeping enzyme because it synthesizes oleate a well-known fatty acid that is abundant in many dietary sources. However numerous studies have shown that SCD is a very highly regulated enzyme that features in so many physiological processes ranging from fat differentiation, carbohydrate and fat metabolism, inflammation and cancer. The editor's studies using stearoyl-CoA desaturase knockout (SCD1-/- ) mice and studies of other investigators using pharmacological approaches to reduce SCD1 expression in mouse tissues have all established that the expression of SCD1 gene isoform represents a key step in partitioning of lipids between storage and oxidation. High SCD expression favors fat storage leading to obesity while reduced SCD expression favors fat burning and leanness. Although these studies clearly illustrated that SCD1 expression is involved in the development of obesity and insulin resistance, questions remain in the elucidation of the mechanisms involved and role of SCD1. This book includes chapters by leading researchers on SCD Genes in the brain, heart, muscle, liver metabolism, Colitis, and more.
  • Store-operated Ca2+ entry (SOCE) serves to control essential functions throughout the human body and represents a novel and attractive target for therapeutic intervention. This book provides an extensive overview of the role of SOCE pathways in Molecular Physiology and Cell Biology, as well as their clinical significance. (Patho)physiological principles and emerging therapeutic strategies are delineated in a way that is valuable both for the education of graduate students in advanced Cell Biology/Molecular Physiology and for the promotion of innovative research and developments in the clinical/therapeutic fields. A comprehensive, clear and elaborate representation of current concepts is provided, including a pathophysiological section arranged in a tissue/organ/system-oriented manner. The book is intended for basic researchers specializing in cell signaling, ion transport, or pharmacology, as well as biomedical scientists and clinicians with a focus on immunology, neurology or cardiology.
  • 1. Stress-induced mutagenesis in bacteria / Ivan Matic -- 2. Mutagenesis associated with repair of DNA double-strand breaks under stress / Chandan Shee, P.J. Hastings, and Suman M. Rosenberg -- 3. Transcription-mediated mutagenic processes / Eduardo Robleto, Holly A. Martin, Carmen Vallin, Mario Pedraza-Reyes and Ronald Yasbin -- 4. Transposon mutagenesis in disease, drug discovery, and bacterial evolution / Zhongge Zhang, Jing Wang, Maksim A. Shlykov, and Milton H. Saier, Jr. -- 5. Hsp90 as a capacitor of both genetic and epigenetic changes in the genome during cancer progression and evolution / Xiangyi Lu, Luan Wang, Vincent E. Sollars, Mark D. Garfinkel, and Douglas M. Ruden -- 6. Inheritance of stress-induced epigenetic changes mediated by the ATF-2 family of transcription factors / Ki-Hyeon Seong, Toshio Maekawa and Shunsuke Ishii -- 7. Microsatellite repeats: canaries in the coalmine / Nimrat Chatterjee, Beatriz A. Santillan and John H. Wilson -- 8. Genetic instability induced by hypoxic stress / Susan E. Scanlon and Peter M. Glazer -- 9. Radiation-induced delayed genome instability and hypermutation in mammalian cells / Christopher P. Allen, Akira Fujimori, Ryuichi Okayasu and Jac A. Nickoloff -- 10. Radiation-induced bystander effects and stress-induced mutagenesis / Carmel Mothersill and Colin Seymour -- 11. Stress induced mutagenesis, genetic diversification, and cell survival via anastais, the reversal of late stage apoptosis / Ho Lam Tang, Ho Man Tang and Denise J. Mitchell -- 12. The transgenerational effects of parental exposure to mutagens in mammals / Yuri E. Dubrova -- 13. Revisiting mutagenesis in the age of High-throughput sequencing / Subhajyoti De and R. Matthew Ward.
  • Complex I (NADH:ubiquinone oxidoreductase) is the first and largest enzyme of the respiratory chain in mitochondria and plays a central role in cellular energy production. Complex I dysfunction has been implicated in many human neurodegenerative diseases, including Parkinson's. Mutations in complex I subunits are among the most common human genetic disorders known. Until recently, in the absence of structural information, complex I was the least understood enzyme of the respiratory chain. The mechanism by which it couples electron transfer between NADH and quinone to the translocation of protons across the membrane was a complete enigma. Recent years have been marked by spectacular progress in the elucidation of complex I's structure, finally allowing the interpretation of a wealth of data accumulated in about 50 years since the discovery of the enzyme. Understanding of the mechanism of this large molecular machine, involving long-range conformational changes, is beginning to emerge. This book is the first devoted entirely to complex I. It contains chapters written by leaders in the field, covering a wide range of topics from the structure of the complex, properties of its many redox centers, subunit composition, mutagenesis studies, evolution of the enzyme and on to the current understanding of the coupling mechanism and the molecular basis for human pathologies. Features: - Concise and authoritative review of current state-of-the-art in research on respiratory complex I - The first book entirely devoted to complex I, a central enzyme in bioenergetics - Recent breakthroughs in structure elucidation finally allow understanding of the mechanism - Mutations leading to common and severe human disorders are discussed.
  • Target selection for structural genomics : an overview / Russell L. Marsden and Christine A. Orengo -- A general target selection method for crystallographic proteomics / Gautier Robin ... [et al.] -- Target selection : triage in the structural genomics battlefield / James Raftery -- Data management in structural genomics : an overview / Sabrina Haquin ... [et al.] -- Data deposition and annotation at the worldwide protein data bank / Shuchismita Dutta ... [et al.] -- Prediction in protein disorder / Zsuzsanna Dosztányi and Peter Tompa -- Protein domain prediction / Helgi Ingolfsson and Golan Yona -- Protein structure modeling with MODELLER / Narayanan Eswar ... [et al.] -- High throughput cloning with restriction enzymes / Volker Sievert, Asgar Ergin, and Konrad Bussow -- Automated recombinant protein expression screening in Escherichia coli / Didier Busso ... [et al.] -- From no expression to high-level soluble expression in Escherichi coli by screening a library of the target proteins with randomized N-termini / Kyoung Hoon Kim ... [et al.] -- Application of high-throughput methodologies to the expression of recombinant proteins in E. coli / Yoav Peleg and Tamar Unger -- A high throughput platform for eukaryotic genes / Yunjia Chen ... [et al.] -- High throughput production of recombinant human proteins for crystallography / Opher Gileadi ... [et al.] -- Assembly of protein complexes by coexpression in prokaryotic and eukaryotic hosts : an overview / Anastassis Perrakis and Christophe Romier -- Cell-free protein synthesis for analysis by NMR spectroscopy / Margit A. Apponyi ... [et al.] -- A medium or high throughput protein refolding assay / Nathan P. Cowieson ... [et al.] -- Structural proteomics of membrane proteins : a survey of published techniques and design of a rational high throughput strategy / Melissa Swope Willis and Christopher M. Koth -- Methods for protein characterization by mass spectrometry, thermal shift (ThermoFluor) assay, and multiangle or static light scattering / Joanne E. Nettleship ... [et al.] -- High throughput methods for analyzing transition metals in proteins on a microgram scale / Anelia Atanassova, Martin Högbom, and Deborah B. Zamble -- High throughput screening of purified proteins for enzymatic activity / Michael Proudfoot ... [et al.] -- Strategies for improving crystallization success rates / Rebecca Page -- Protein crystallization in restricted geometry : advancing old ideas for modern times in structural proteomics / Joseph D. Ng, Raymond C. Stevens, and Peter Kuhn -- Fluorescence approaches to growing macromolecule crystals / Marc Pusey, Elizabeth Forsythe, and Aniruddha Achari -- Efficient macromoleular crystallization using microfluidics and randomized design of screening reagents / Andrew P. May and Brent W. Segelke -- Increasing protein crystallization screening success with heterogeneous nucleating agents / Anil S.Thakur ... [et al.] -- High throughput pH optimization of protein crystallization / Ran Meged, Orly Dym, and Joel L. Sussman -- Automated structure solution with the PHENIX suite / Peter H. Zwart ... [et al.] -- NMR screening for rapid protein characterization in structural proteomics / Justine M. Hill -- Microcoil NMR spectroscopy : a novel tool for biological high throughput NMR spectroscopy / Russell E. Hopson and Wolfgang Peti -- Protein structure determination using a combination of cross-linking, mass spectrometry, and molecular modeling / Dmitri Mouradov ... [et al.] -- Structural genomics of minimal organisms : pipeline and results / Sung-Hou Kim ... [et al.] -- Structural genomics of pathogenic protozoa : an overview / Erkang Fan ... [et al.] -- High throughput crystallography at SGC Toronto : an overview / Alexey Bochkarev and Wolfram Tempel -- The structural biology and genomics platform in Strasbourg : an overview / Didier Busso, Jean-Claude Thierry, and Dino Moras -- Bacterial structural genomics initiative : overview of methods and technologies applied to the process of structure determination / Miroslaw Cygler ... [et al.] -- High-throughput protein production and crystallization at NYSGXRC / Michael J. Sauder ... [et al.] -- Overview of the pipeline for structural and functional characterization of macrophage proteins at the University of Queensland / Weining Meng ... [et al.] -- Structural genomics of the bacterial mobile metagenome : an overview / Andrew Robinson ... [et al.].
  • Principles of protein structure and function -- A brief history of protein disorder -- Indirect techniques for recognizing and characterizing protein disorder -- Hydrodynamic techniques -- Spectroscopic techniques for characterizing disorder -- Nuclear magnetic resonance -- Proteomic approaches for the identification of IDPs -- IDPs under conditions approaching in vivo -- Prediction of disorder -- Structure of IDPs -- Biological processes enriched in disorder -- Molecular functions of disordered proteins -- Evolution and prevalence of disorder -- Extension of the structure-function paradigm -- Structural disorder and disease.
  • This book contemplates the structure, dynamics and physics of virus particles: From the moment they come into existence by self-assembly from viral components produced in the infected cell, through their extracellular stage, until they recognise and infect a new host cell and cease to exist by losing their physical integrity to start a new infectious cycle. (Bio)physical techniques used to study the structure of virus particles and components, and some applications of structure-based studies of viruses are also contemplated. This book is aimed first at M.Sc. students, Ph.D. students and postdo
  • Superoxide distumase 2002, ScienceDirect
  • Taurine. 6 2006, Springer
  • Taurine. 7 2009, Springer
  • Taurine. 8 v. 1-2, 2013., Springer
    v.2 Springer
    Volume 1. The Nervous system, immune system, diabetes and the cardiovascular system -- Volume 2. Nutrition and metabolism, protective role, and role in reproduction, development, and differientiation.
  • Introduction: The GW Body Story as an Example of Autoantibodies with Significant Impacts to Molecular Cell Biology -- The Discovery of GW Bodies -- The Discovery and Analysis of P Bodies -- Autoantibodies to Argonaute 2 (Su Antigen) -- GW/P-Bodies and Autoimmune Disease -- Function of GW182 and GW Bodies in siRNA and miRNA Pathways -- Post-transcriptional Stimulation of Gene Expression by MicroRNAs -- Gawky (GW) is the Drosophila melanogaster GW182 Homologue -- The Role of GW182 Proteins in miRNA-Mediated Gene Silencing -- Quantifying Argonaute Proteins In and Out of GW/P-Bodies: Implications in microRNA Activities -- Deadenylation and P-Bodies -- Relationship of GW/P-Bodies with Stress Granules -- Relationship of Other Cytoplasmic Ribonucleoprotein Bodies (cRNPB) to GW/P Bodies -- An SNP in the Trinucleotide Repeat Region of the TNRC6A Gene Maps to a Major TNGW1 Autoepitope in Patients with Autoantibodies to GW182 -- Reflections on Ten Years of History of, and Future Prospects for, GW182 and GW/P Body Research.
  • An historical introduction to porphyrin and chlorophyll synthesis / Michael R. Moore -- Biosynthesis of 5-aminolevulinic acid / Dieter Jahn and Dirk W. Heinz -- 5-aminolaevulinic acid dehydratase, porphobilinogen deaminase and uroporphyrinogen III synthase / Heidi L. Schubert, Peter T. Erskine and Jonathan B. Cooper -- Transformation of uroporphyrinogen III into protohaem / Johanna E. Cornah and Alison G. Smith -- Inherited disorders of haem synthesis: the human porphyrias / Michael N. Badminton and George H. Elder -- Heme degradation: mechanistic and physiological implications / Angela Wilks -- Regulation of mammalian heme biosynthesis / Amy E. Medlock and Harry A. Dailey -- Tetrapyrroles in photodynamic therapy / David I. Vernon and Ian Walker -- Heme transport and incorporation into proteins / Linda Thöny-Meyer -- Heme and hemoproteins / Andrew W. Munro ... [et al.] -- Novel heme-protein interactions: some more radical than others / Ann Smith -- Synthesis and role of bilins in photosynthetic organisms / Nicole Frankenberg-Dinkel and Matthew J. Terry -- Phytochromes: bilin-linked photoreceptors in bacteria and plants / Matthew J. Terry and Alex C. McCormac -- Biosynthesis of chlorophyll and bacteriochlorophyll / Derren J. Heyes and C. Neil Hunter -- Regulation of tetrapyrrole synthesis in higher plants / Matthew J. Terry and Alison G. Smith -- Regulation of the late steps of chlorophyll biosynthesis / Wolfhart Rudiger -- Chlorophyll breakdown / Bernhard Kräutler -- Vitamin B12: biosynthesis of the corrin ring / Ross M. Graham, Evelyne Deery and Martin J. Warren -- Conversion of cobinamide into coenzyme B12 / Jorge C. Escalante-Semerena ... [et al.] -- The regulation of cobalamin biosynthesis / Jeffrey G. Lawrence -- Coenzyme B12-catalyzed radical isomerizations / Dominique Padovani and Ruma Banerjee -- Biosynthesis of siroheme and coenzyme F430 / Martin J. Warren, Evelyne Deery and Ruth-Sarah Rose -- Role of coenzyme F430 in methanogenesis / Evert C. Duin -- The role of siroheme in sulfite and nitrite reductases / M. Elizabeth Stroupe and Elizabeth D. Getzoff -- The role of heme d1 in denitrification / Stuart J. Ferguson.
  • Tetraspanins 2013, Springer
    Tetraspanin proteins have recently emerged as a new class of modulators of various processes involving cell surface receptors, including cell migration and invasion, host immune responses, cell-cell fusion, and viral infection. The book summarises recent advances in the fields of biology in which the role of tetraspanins have been established and also covers the molecular evolution of the tetraspanin superfamily and structural aspects of the organisation of tetraspanin microdomains.
  • "Therapeutic applications of quadruplex nucleic acids provides a single comprehensive survey that describes and assesses recent advances in quadruplex therapeutics and targeting strategies. It also covers the underlying fundamentals of such topics as quadruplex structure, small-molecule recognition, biological roles of genomic quadruplexes, and quadruplex informatics"--P. 4 of cover.
  • Biopharmaceutical proteins: opportunities and challenges / John R. Birch and Yemi Onakunle -- Expression of antibody fragments by periplasmic secretion in Escherichia coli / Andrew G. Popplewell ... [et al.] -- Secretory production of therapeutic proteins in Escherichia coli / Sang Yup Lee, Jong Hyun Choi, and Sang Jun Lee -- Expression of recombinant LTB protein in marine vibrio VSP60 / Wang Yili and Si Lusheng -- Heterologous gene expression in yeast / Lee J. Byrne, Kenneth J. O'Callaghan, and Mick F. Tuite -- Pharmaceutical proteins from methylotrophic yeasts / Eric C. de Bruin ... [et al.] -- Expression of human papillomavirus type 16 L1 in baculovirus expression systems: a case study / Zheng Jin, Si Lusheng, and Wang Yili -- Large-scale transient expression of therapeutic proteins in mammalian cells / Sabine Geisse, Martin Jordan, and Florian M. Wurm -- Site-specific integration for high-level protein production in mammalian cells / Bhaskar Thyagarajan and Michele P. Calos -- Production of recombinant therapeutic proteins by mammalian cells in suspension culture / Lily Chu, Ilse Blumentals, and Gargi Maheshwari -- Transgene control engineering in mammalian cells / Beat P. Kramer and Martin Fussenegger -- Fusion to albumin as a means to slow the clearance of small therapeutic proteins using the Pichia pastoris espression system: a case study / William P. Sheffield, Teresa R. McCurdy, and Varsha Bhakta -- High throughput recovery of therapeutic proteins from the inclusion bodies of Escherichia coli: an overview / Amulya K. Panda -- Isolation, solubilization, refolding, and chromatographic purification of recombinant human growth hormone from inclusion bodies of Escherichia coli cells: a case study / Surinder M. Singh ... [et al.] -- Large-scale preparation of factor VIIa from human plasma: a case study / Teruhisa Nakashima and Kazuhiko Tomokiyo -- Purification of clinical-grade monoclonal antibodies by chromatographic methods / Alberto L. Horenstein, Ilaria Durelli, and Fabio Malavasi -- Virus elimination and validation / Nicola Boschetti and Anna Johnston -- Virus removal by nanofiltration / Marina Korneyeva and Scott Rosenthal -- Determining residual host cell antigen levels in purified recombinant proteins by slot blot and scanning laser densitometry / Aaron P. Miles, Daming Zhu, and Allan Saul -- Principles of biopharmaceutical protein formulation: an overview / Scott P. Sellers and Yuh-Fun Maa -- Solid-state protein formulation: methodologies, stability, and excipient effects / Yuh-Fun Maa and Scott P. Sellers -- Stabilization of therapeutic proteins by chemical and physical methods / Ken-ichi Izutsu -- Extraction and characterization of vaccine antigens from water-in-oil adjuvant formulations / Aaron P. Miles and Allan Saul -- Probing reversible self-association of therapeutic proteins by sedimentation velocity in the analytical ultracentrifuge / Bernardo Perez-Ramirez and John J. Steckert -- Biological characterization of pegylated interferons: a case study / Lei Xie ... [et al.] -- Characterization of interferon [alpha]-2B pegylated via carboxyalkylation: a case study / David C. Wylie ... [et al.] -- Quantifying recombinant proteins and their degradation products using SDS-PAGE and scanning laser densitometry / Aaron P. Miles and Allan Saul -- Extraction and denaturing gel electrophoretic methodology for the analysis of yeast proteins / Kenneth J. O'Callaghan, Lee J. Byrne, and Mick F. Tuite -- Characterization of therapeutic proteins by membrane and in-gel tryptic digestion / C. Mark Smales, Rosalyn J. Marchant, and Michèle F. Underhill -- Oligosaccharide release and MALDI-TOF MS analysis of N-linked carbohydrate structures from glycoproteins / Rodney G. Keck, John B. Briggs, and Andrew J.S. Jones -- Capillary electrophoresis of carbohydrates derivatized with fluorophoric compounds / Stacey Ma ... [et al.] -- High-throughput LC/MS methodology for [alpha](1->3)Gal determination of recombinant monoclonal antibodies: a case study / Lihua Huang and Charles E. Mitchell -- Carbohydrate structural characterization of Fas ligand inhibitory protein / Lihua Huang ... [et al.] -- Top-down characterization of protein pharmaceuticals by liquid chromatography/mass spectrometry: application to recombinant factor IX comparability-a case study / Jason C. Rouse ... [et al.] -- Sample preparation procedures for high-resolution nuclear magnetic resonance studies of aqueous and stabilized solutions of therapeutic peptides / Mark J. Howard.
  • "Thermostable Proteins: Structural Stability and Design provides a comprehensive, updated account of the physical basis of enhanced stability of thermophilic proteins and the design of tailor-made thermostable proteins, paving the way for their possible industrial applications. This book is devoted to understanding the survival mechanisms of "thermophilic life forms" at the molecular level with an emphasis on design strategies.The review chapters presented in Thermostable Proteins span a wide range of protein thermostability research. Basic structural, thermodynamic, and kinetic principles are explained and molecular strategies for the adaptation to high temperatures are delineated. In addition, this book covers: computing and simulation methods in current and future thermostability research, especially in nonempirical situations, how rigidity theory is used to improve the thermal adaptation of mesophiles Subtilisin-like serine proteases and their significant engineering applications. The state of knowledge concerning structure function relations and the origins of their structural stability. Computational and experimental approaches for the design of proteins with increased thermal stability based on sequences or three-dimensional structures. Understanding the molecular basis of how thermostable and hyperthermostable proteins gain and maintain their stability and biological function at high temperatures remains an important scientific challenge. A more detailed knowledge of protein stability not only deepens our understanding of protein structure but also helps in obtaining insights into processes that drive protein activities folding, unfolding, and misfolding essential to biological function"--Provided by publisher.
  • Changing paradigms in thiology: from antioxidant defense toward redox regulation / Leopold Flohé -- Mass spectrometry-based methods for the determination of sulfur and related metabolite concentrations in cell extracts / Emmanuel Godat ... [et al.] -- Use of dimedone-based chemical probes for sulfenic acid detection: evaluation of conditions affecting probe incorporation into redox-sensitive proteins / Chananat Klomsiri ... [et al.] -- Use of dimedone-based chemical probes for sulfenic acid detection: methods to visualize and identify labeled proteins / Kimberly J. Nelson ... [et al.] -- Formation and reactions of sulfenic acid in human serum albumin / Beatriz Alvarez ... [et al.] -- Determination of GSH, GSSG, and GSNO using HPLC with electrochemical detection / Li-Peng Yap ... [et al.] -- Measurement of mixed disulfides including glutathionylated proteins / Raffaella Priora ... [et al.] -- Detection and quantification of protein disulfides in biological tissues: a fluorescence-based proteomic approach / Viviana I. Pérez ... [et al.] -- Measurement and identification of S-glutathiolated proteins / Bradford G. Hill ... [et al.] -- Proteome screens for Cys residues oxidation: the redoxome / Giovanni Chiappetta ... [et al.] -- Identification by MS/MS of disulfides produced by a functional redox transition / Pierluigi Mauri ... [et al.] -- Mass spectrometry approaches for the redox characterization of protein cysteine residues: the case of the transcription factor Pax-8 / Andrea Scaloni and Gianluca Tell -- A simple method to systematically study oxidatively modified proteins in biological samples and its applications / Byoung-Joon Song, Soo-Kyung Suh and Kwan-Hoon Moon -- Direct and indirect detection methods for the analysis of S-nitrosylated peptides and proteins / Federico Torta, Lisa Elviri and Angela Bachi -- A rapid approach for the detection, quantification, and discovery of novel sulfenic acid or S-nitrosothiol modified proteins using a biotin-switch method / Joseph R. Burgoyne and Philip Eaton -- Protein addicts of aldehydic lipid peroxidation products: identification and characterization of protein adducts using an aldehyde/keto-reactive probe in combination with mass spectrometry / Claudia S. Maier ... [et al.].
  • Engineering of Fluorescent Reporters into Redox Domains to Monitor Electron Transfers / Derek Parsonage ... [et al.] -- Blot-Based Detection of Dehydroalanine-Containing Glutathione Peroxidase with the Use of Biotin-Conjugated Cysteamine / Sue Goo Rhee, Chun-Seok Cho -- Analysis of the Redox Regulation of Protein Tyrosine Phosphatase Superfamily Members Utilizing a Cysteinyl-Labeling Assay / Benoit Boivin, Nicholas K. Tonks -- Measuring the Redox State of Cellular Peroxiredoxins by Immunoblottin / Andrew G. Cox, Christine C. Winterbourn, Mark B. Hampton -- Thiol Redox Transitions by Thioredoxin and Thioredoxin-Binding Protein-2 in Cell Signaling / Eiji Yoshihara ... [et al.] -- Detection of Protein Thiols in Mitochondrial Oxidative Phosphorylation Complexes and Associated Proteins / Kelly K. Andringa, Shannon M. Bailey -- Mitochondrial Thioredoxin Reductase: Purification, Inhibitor Studies, and Role in Cell Signaling / Maria Pia Rigobello, Alberto Bindoli -- Measuring Mitochondrial Protein Thiol Redox State / Raquel Requejo ... [et al.] -- Measurement of Extracellular (Exofacial) Versus Intracellular Protein Thiols / Jolanta Skalska, Steven Bernstein, Paul Brookes -- Redox Clamp Model for Study of Extracellular Thiols and Disulfides in Redox Signaling / Young-Mi Go, Dean P. Jones -- Redox State of Human Serum Albumin in Terms of Cysteine-34 in Health and Disease / Karl Oettl, Gunther Marsche -- Methods for Studying Redox Cycling of Thioredoxin in Mediating Preconditioning-Induced Survival Genes and Proteins / Chuang C. Chiueh -- Oxidative Stress, Thiol Redox Signaling Methods in Epigenetics / Isaac K. Sundar ... [et al.] -- Characterization of Protein Targets of Mammalian Thioredoxin Reductases / Anton A. Turanov, Dolph L. Hatfield, Vadim N. Gladyshev -- Alteration of Thioredoxin Reductase 1 Levels in Elucidating Cancer Etiology / Min-Hyuk Yoo ... [et al.] -- Regulation of Apoptosis Signal-Regulating Kinase 1 in Redox Signaling / Kazumi Katagiri, Atsushi Matsuzawa, Hidenori Ichijo -- Protocols for the Detection of S-Glutathionylated and S-Nitrosylated Proteins In Situ / Scott W. Aesif, Yvonne M.W. Janssen-Heininger, Niki L. Reynaert -- Synthesis, Quantification, Characterization, and Signaling Properties of Glutathionyl Conjugates of Enals / Sanjay Srivastava ... [et al.] -- Thioredoxin and Redox Signaling in Vasculature--Studies Using Trx2 Endothelium-Specific Transgenic Mice / Wang Min ... [et al.]
  • Applications of tissue microarray technology -- Quality aspects of TMA analysis -- Representativity of TMA studies -- Recipient block TMA technique -- Protocol for constructing tissue arrays by cutting edge matrix assembly -- Hypodermic needle without recipient paraffin block technique -- Resin technologies : construction and staining of resin TMA's -- Tissue microarrays from frozen tissues-OCT technique -- An alternative technology to prepare tissue microarray using frozen tissue samples -- Building "tissue" microarrays from suspension cells -- Tissue microarrays from biopsy specimens -- Immunohistochemical analysis of tissue microarrays -- DNA copy number analysis on formalin-fixed paraffin-embedded tissues in TMA format by RNA in situ hybridization -- Automated analysis of tissue microarrays -- Digital microscopy for boosting database integration and analysis in TMA studies -- From gene to clinic : TMA-based clinical validation of molecular markers in prostate cancer
  • "A New Avenue of Research Beyond Traditional Studies of Vitamin E. The first 90 years of vitamin E research has produced prolific and notable discoveries. Until the last few decades, attention has been given mostly to the biological activities and underlying mechanisms of alpha-tocopherol, which we now know is one of more than eight vitamin E isomers. Tocotrienol discovery came much later, in 1965, but existed for almost 20 years as an obscure vitamin E. Explores Treatment Possibilities: Tocotrienols have proven to contain some exceptional benefits that aren't shared by the better known tocopherols. Although both are antioxidants, today a bright spot for tocotrienol research is in cancer and heart disease, while emergent fields of tocotrienol research are on the rise with many covered in this edition. Currently, tocotrienols have reached a new measure of research height: more than one-third of all vitamin E tocotrienol research of the last 30 years was published in the last 3 years. The thriving field of tocotrienol research gives ground for publication of this 2nd edition, a compilation of the latest tocotrienol research in all new chapters. For the past 10 years, Professors Ronald R. Watson and Victor R. Preedy have published books on bioactive nutrients and dietary supplements. Professor Watson has been and is currently funded to do research by grants from the American Heart Foundation and the U.S. National Institute of Heart, Lung, and Blood. By gathering the contributions contained in this volume, they have expanded the field of knowledge and forged a new path in disease treatment and prevention"--Provided by publisher.
  • Mammalian transglutaminases : a family portrait / Mehta, K. -- Structure-function relationships of transglutaminases, a contemporary view / Nemes, Z., Csosz, E., Fesus, L. -- Transglutaminases and their substrates / Facchiano, F., Facchiano, A. -- Type 2 transglutaminase and cell death / Piacentini, M. ... [et al.] -- Transglutaminase and cell-survival signaling / Singh, U.S., Pan, J. -- Transglutaminases in wound healing and inflammation / Verderio, E.A.M., Johnson, T.S., Griffin, M. -- Transglutaminases in epidermis / Eckert, R.L. ... [et al.] -- Tissue transglutaminase (TG2) in cancer biology / Mangala, L.S., Mehta, K. -- Transglutaminases in neurodegenerative disorders / Bailey, C.D.C., Tucholski, J., Johnson, G.V.W. -- Tissue transglutaminase and celiac disease / Esposito, C. ... [et al.] -- Transglutaminases as biotechnological tools / Mariniello, L., Porta, R. -- Extracellular transglutaminase: factor XIII / Ichinose, A. -- Transglutaminases of lower organisms / Rao, R.U., Chandrashekar, R., Mehta, K. -- Transglutaminases of higher, lower plants and fungi / Del Duca, S., Serafini-Fracassini, D.
  • Insights into Structural Basis of Mammalian Mitochondrial Translation / Manjuli R. Sharma, Prem S. Kaushal -- Mechanism and Regulation of Protein Synthesis in Mammalian Mitochondria / Emine C. Koc, Hasan Koc -- Translation in Mammalian Mitochondria: Order and Disorder Linked to tRNAs and Aminoacyl-tRNA Synthetases / Catherine Florentz, Joern Pütz -- Mitochondrial Targeting of RNA and Mitochondrial Translation / Ivan Tarassov, Ivan Chicherin, Yann Tonin -- Mechanisms and Control of Protein Synthesis in Yeast Mitochondria / Steffi Gruschke, Martin Ott -- Kinetoplast-Mitochondrial Translation System in Trypanosomatids / Dmitri A. Maslov, Rajendra K. Agrawal -- Translation in Mitochondria and Apicoplasts of Apicomplexan Parasites / Ankit Gupta, Afreen Haider, Suniti Vaishya -- Mitochondrial Translation in Green Algae and Higher Plants / Thalia Salinas, Claire Remacle -- Translation in Chloroplasts of Flowering Plants / Masahiro Sugiura -- The Chloroplasts as Platform for Recombinant Proteins Production / Nunzia Scotti, Michele Bellucci.
  • Use of reticulocyte lysates for mechanistic studies of eukaryotic translation initiation / William C. Merrick and Diane Barth-Baus -- Studying translational control in Drosophila cell-free systems / Fátima Gebauer and Matthias W. Hentze -- Use of in vitro translation extract depleted in specific initiation factors for the investigation of translational regulation / Daniel R. Gallie -- A highly efficient and robust in vitro translation system for expression of picornavirus and hepatitis C virus in RNA genomes / Yuri V. Svitkin and Nahum Sonenberg -- A practical approach to isolate 48S complexes : affinity purification and analyses / Nicolas Locker and Peter J. Lukavsky -- Yeast phenotypic assays on translational control / Bumjun Lee ... [et al.] -- Localization and characterization of protein-protein interaction sites / Chingakham Ranjit Singh and Katsura Asano -- In vivo stabilization of preinitiation complexes by formaldehyde cross-linking / Leos Valás̆ek ... [et al.] -- Molecular genetic structure-function analysis of translation initiation factor elF5B / Byung-Sik Shin and Thomas E. Dever -- The use of fungal in vitro systems for studying translational regulation / Cheng Wu ... [et al.] -- Investigating translation initiation using drosophila molecular genetics / Gritta Tettweiler and Paul Lasko -- Analysis of RNA:protein interactions in vivo : identification of RNA-binding partners of nuclear factor 90 / Andrew M. Parrott, Melissa R. Walsh, and Michael B. Mathews -- Approaches for analyzing the differential activities and functions of elF4E family members / Robert E. Rhoads, Tzvetanka D. Dinkova, and Rosemary Jagus -- Tethered function assays : an adaptable approach to study RNA regulatory proteins / Jeff Coller and Marv Wickens -- Analysis of ribosomal shunting during translation initiation in eukaryotic mRNAs / Vincent P. Mauro, Stephen A. Chappell and John Dresios.
  • Transient kinetics, flourescence, and FRET in studies of initiation and translation in bacteria / Pohl Milon ... [et al.] -- Binding of mRNA to the bacterial translation initiation complex / Sean M. Studer and Simpson Joseph -- Real-time dynamics of ribosome-ligand interaction by time-resolved chemical probing methods / Attilio Fabbretti ... [et al] -- Overexpression and purification of mammalian mitochondrial translational initiation factor 2 and initiation factor 3 / Domenick G. Grasso ... [et al.] -- In vitro studies of archaeal translational initiation / Dario Benelli and Paola Londei -- Reconstitution of yeast translation initiation / Michael G. Acker ... [et al.] -- Assembly and analysis of eukaryotic translation initiation complexes / Andrey V. Pisarev -- Reconstitution of mammalian 48S ribosomal translation initiation complex / Romit Majumdar, Jayanta Chaudhuri, and Umadas Maitra -- Biophysical approach to studies of Cap-elF4E interaction by synthetic cap analogs / Anna Niedzwiecka ... [et al.] -- Biophysical studies of the translation initiation pathway with immobilized mRNA analogs / John E. G. McCarthy, Steven Marsden, and Tobias von der Haar -- Protection-based assays to measure aminoacyl-tRNA binding to translation initiation factors / Yves Mechulam ... [et al.] -- NMR methods for studying protein-protein interactions involved in translation initiation / Assen Marintchev, Dominique Frueh, and Gerhard Wagner -- Structural methods for studying IRES function / Jeffrey S. Kieft ... [et al.].
  • TRIM/RBCC proteins 2012, Springer
    Genomics and evolution of the TRIM gene family / Germana Meroni -- The tripartite motif : structure and function / Lucia Micale ... [et al.] -- TRIM proteins as ring finger E3 ubiquitin ligases / Kazuhiro Ikeda and Satoshi Inoue -- PML nuclear bodies and other TRIM-defined subcellular compartments / Elizabeth C. Batty, Kirsten Jensen, and Paul S. Freemont -- TRIM involvement in transcriptional regulation / Florence Cammas ... [et al.] -- TRIM proteins in cancer / Valeria Cambiaghi ... [et al.] -- TRIM proteins and the innate immune response to viruses / Melvyn W. Yap and Jonathan P. Stoye -- The microtubule-associated C-I subfamily of TRIM proteins and the regulation of polarized cell responses / Timothy C. Cox -- MuRFS : specialized members of the TRIM/RBCC family with roles in the regulation of the trophic state of muscle and its metabolism / Olga Mayans and Siegfried Labeit -- Trim proteins in development / Francesca Petrera and Germana Meroni.
  • Tropomyosin 2008, Springer
    Introduction and historical perspective / Peter Gunning -- Structure and evolution of tropomyosin genes / Bernadette Vrhovski, Nadine Thézé, and Pierre Thiébaud -- Tropomyosin exons as models for alternative splicing / Clare Gooding and Christopher W.J. Smith -- Tropomyosin gene expression in vivo and in vitro / Galina Schevzov and Geraldine O'Neill -- Tropomyosin : function follows structure / Sarah E. Hitchcock-DeGregori -- Dimerization of tropomyosins / Mario Gimona -- Cooperative binding of tropomyosin to actin / Larry S. Tobacman -- Tropomyosin and the steric mechanism of muscle regulation / William Lehman and Roger Craig -- Role of tropomyosin in the regulation of contraction in smooth muscle / Steve Marston and M. El-Mezgueldi -- Tropomyosin as a regulator of cancer cell transformation / David M. Helfman ... [et al.] -- The role of tropomyosin in heart disease / David F. Wieczorek, Ganapathy Jagatheesan, and Sudarsan Rajan -- Tropomyosins in skeletal muscle diseases / Anthony J. Kee and Edna C. Hardeman -- Tropomyosins in human diseases : ulcerative colitis / Kiron M. Das and Manisha Bajpai -- Tropomyosin function in yeast / David Pruyne -- Isoform sorting of tropomyosins / Claire Martin and Peter Gunning -- Human tropomyosin isoforms in the regulation of cytoskeleton functions / Jim Jung ... [et al.] -- Tropomyosins regulate the impact of actin binding proteins on actin filaments / Uno Lindberg ... [et al.] -- Tropomyosin and ADF/cofilin as collaborators and competitors / Thomas B. Kuhn and James R. Bamburg -- Caldesmon and the regulation of cytoskeletal functions / C.L. Albert Wang -- Tropomyosins as discriminators of myosin function / E. Michael Ostap -- Tropomodulin/tropomyosin interactions regulate actin pointed end dynamics / Alla S. Kostyukova -- Emerging issues for tropomyosin structure, regulation, function, and pathology / Peter Gunning.
  • TRP channels 2011, CRCnetBASE
    "Transient Receptor Potential (TRP) channels have gained a lot of popularity in recent years due to their involvement in a wide variety of cellular functions in many different systems. The purpose of this book is to provide detailed methods for the study of TRP channels. It includes expression and functional studies of TRP channels in heterologous systems as well as structural and functional studies of TRP channels. The author also explores various methods for detailed analysis of biophysical properties of TRP channels. In addition, the text covers high-throughput screening assays for TRP channels and offers novel and effective approaches to study endogenous TRP channel function in native systems"--Provided by publisher.
  • Two-dimensional electrophoresis : an overview / Richard Smith -- Solubilization of proteins in 2DE : an outline / Thierry Rabilloud -- Selection of pH ranges in 2DE / Mireille Starita-Geribaldi -- Difficult proteins / Ben Herbert and Elizabeth Harry -- Organelle proteomics / Matthias Plöscher ... [et al.] -- Applications of chemical tagging approaches in combination with 2DE and mass spectrometry / Alexander Leitner and Wolfgang Lindner -- Immunoblotting 2DE membranes / Brian McDonagh -- Troubleshooting image analysis in 2DE / Bettina Levänen and Åsa M. Wheelock -- Analysis of bacterial proteins by 2DE / Philip Cash and Evelyn Argo -- Proteomic analysis of Caenorhabditis elegans / Pan-Young Jeong ...[et al.] -- Protein extraction for 2DE / Claus Zabel and Joachim Klose -- Analysis of proteins from marine molluscs / Suze Chora, Maria João Bebianno, and Michèle Roméo -- Preparation and analysis of plastid proteomes by 2DE / Anne von Zychlinski and Wilhelm Gruissem -- High resolution 2DE / Katrin Marcus ...[et al.] -- Blue native-gel electrophoresis proteomics / Kelly Andringa, Adrienne King, and Shannon Bailey -- 2DE for proteome analysis of human metaphase chromosomes / Kiichi Fukui and Susumu Uchiyama -- Microsomal proteomics / Diana M. Wong and Khosrow Adeli -- Prefractionation using microscale solution IEF / Won-A Joo and David Speicher -- Diagonal electrophoresis for detection of protein disulphide bridges / Brian McDonagh -- High-resolution large-gel 2DE / Claus Zabel and Joachim Klose -- Silver staining of proteins in 2DE gels / Cécile Lelong ...[et al.] -- Detection of 4-hydroxy-2-nonenal- and 3-nitrotyrosine-modified proteins using a proteomics approach / Rukhsana Sultana, Tanea Reed, and D. Allan Butterfield -- Proteomic detection of oxidized and reduced thiol proteins in cultured cells / Sarah L. Cuddihy ...[et al.] -- Detection of ubiquitination in 2DE / Brian McDonagh -- Phosphoroproteome analysis by in-gel isoelectric focusing and tandem mass spectrometry / Sarka Beranova-Georgianni, Dominic M. Desiderio, and Francesco Giorgianni -- Detection of protein glutathionylation / Elisabetta Gianazza, Ivano Eberini, and Pietro Ghezzi -- Activity-based protein profiling of protein tyrosine phosphatases / Chad Walls, Bo Zhou, and Zhong-Yin Zhang -- Active protease mapping in 2DE gels / Zhenjun Zhao and Pamela J. Russell -- Two-dimensional difference gel electrophoresis / Gert Van den Bergh -- Protein expression profiling / Brian P. Bradley, Bose Kalampanayil, and Michael C. O'Neill -- C-terminal sequence analysis of 2DE-separated proteins / Bart Samyn, Kjell Sergeant, and Jozef Van Beeumen -- Shotgun protein analysis by liquid chromatography-tandem mass spectrometry / Kazuishi Kubota, Toshiyuki Kosaka, and Kimihisa Ichikawa --De novo sequence analysis of N-terminal sulfonated peptides after in-gel guanidination / Kjell Sergeant, Jozef Van Beeumen, and Bart Samyn -- Tryptic digestion of in-gel proteins for mass spectrometry analysis / Mai-Loan Huynh, Pamela Russell, and Bradley Walsh -- Database interrogation algorithms for identification of proteins in proteomic separations / Patricia M. Palagi, Frédérique Lisacek, and Ron D. Appel -- Creating 2DE databases for the world wide web / Christine Hoogland, Khaled Mostaguir, and Ron D. Appel.
  • Ubiquitin and protein degradation. Part A-B pt. A-B, 2005., ScienceDirect
    pt. B ScienceDirect
  • Ubiquitin-proteasome protocols 2005, Springer Protocols
    Assays of proteasome-dependent cleavage products / Stefan Tenzer and Hansjörg Schild -- Identification of components of protein complexes / Carol E. Parker ... [et al.] -- Mass spectroscometric determination of protein ubiqitination / Carol E. Parker ... [et al.] -- Reconstitution of endoplasmic reticulum-associated degradation using yeast membranes and cytosol / Robert J. Lee, Ardythe A. McCracken, and Jeffrey L. Brodsky -- Reticulocyte lysate as a model system to study endoplasmic reticulum membrane protein degradation / Eric Carlson ... [et al.] -- Deubiquitinating enzyme purifucation, assay inhibitors and characterization / Nathaniel S. Russell and Keith D. Wilkinson -- Measuring ubiquitin conjugation in cells / Edward G. Mimnaugh and Leonard M. Neckers -- Assays for proteasome assembly and maturation / R. Jürgen Dohmen ... [et al.] -- N-terminal ubiquitination / Aaron Ciechanover -- Quantitating defective ribosome products / Shu-Bing Qian, Jack R. Bennink, and Jonathan W. Yewdell -- Endoplasmic reticulum-associated protein quality control and degradation: screen for ERAD mutants after ethylmethane sulfonate mutagenesis / Antje Schäfer and Dieter H. Wolf -- Endoplasmic reticulum-associated protein quality control and degradation: genome wide screen for ERAD components / Antje Schäfer and Dieter H. Wolf -- Cystic fibrosis transmembrane conducatance regulator as a model substrate to study endoplasmic reticulum protein quality control in mammalian cells / J. Michael Younger ... [et al.] -- Aggresome formation / Michael J. Corboy, Philip J. Thomas, and W. Christian Wigley -- Detection of sumoylated proteins / Roland S. Hilgarth and Kevin D. Sarge -- Proteasome inhibitors in cancer therapy / Robert Z. Orlowski -- Parkinson disease: assays for the ubiquitin ligase activity of neural parkin / Michael G. Schlossmacher and Hideki Shimura. Small-molecule inhibitors of proteasome activity / Maria Gaczynska and Pawel A. Osmulski -- Purification of E1 and E1-like enzymes / Arthur L. Haas -- Assays for RING family ubiquitin ligases / Manabu Furukawa, Paul S. Andrews, and Yue Xiong -- Ubiquitin chain synthesis / Shahri Raasi and Cecile M. Pickart -- Purification of proteasomes, proteasome subcomplexes, and proteasome-associated proteins from budding yeast / David S. Leggett, Michael H. Glickman, and Daniel Finley -- Recognition and processing of misfolded proteins by PA700, the 19S regulatory complex of the 26S proteasome / Chang-Wei Liu ... [et al.] -- Cell-free assay for ubiquitin-independent proteasomal protein degradation / Chaim Kahana and Yuval Reiss --
  • pt. B ScienceDirect
    pt. C ScienceDirect
  • Urea transporters 2014, Springer
    The mechanisms and physiological functions of urea transport across biological membranes are subjects of long-standing interest. Recent advances in the molecular biology and physiology of urea transport have yielded new insights into how and why urea moves across cell membranes. In the last two decades, seven facilitated urea transporters (UT-A1-6 and UT-B) have been cloned, and their gene organization, protein crystal structure, expression localization and physiological functions in the tissues have been described. In recent years, the studies in urea transporter knockout mouse models suggest that urea transporters may be useful targets for drug discovery of selective inhibitors. The modulation of urea transport activity by pharmacological agents may provide novel treatments for hypertension, congestive heart failure and other fluid-retaining states. However, although urea represents about 40% of all urinary solutes in normal human urine, the handling of this solute in the tissues has been largely neglected in the past, and few clinical or experimental studies now report data about urea. Most recent physiological textbooks include chapters on water and electrolyte physiology but not a single chapter on urea. Our aim in writing this book is to stimulate further research in new directions by providing novel and provocative insights into further mechanisms and the physiological significance of urea metabolism and transport in mammals. The book provides a state-of-the-art report on the latest findings on urea transport and where the field is going. Although some older work is cited, the main focus is on advances made over the past 20 years with regard to the biophysics, genetics, protein structure, molecular biology, physiology, pathophysiology and pharmacology of urea transport in mammalian cell membranes. These aspects are especially valid, as advances in our understanding of urea transporting mechanisms and physiology promise to yield new insights into biology and medicine.
  • This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells.
  • Clathrin-Mediated Endocytosis / Alexander Sorkin, Manojkumar A. Puthenveedu -- Intimate and Facultative? Regulation of Clathrin-Mediated Endocytosis by the Actin Cytoskeleton / Tal Hirschhorn, Marcelo Ehrlich -- Oncogenic Signaling from the Plasma Membrane / Eli Zamir, Nachiket Vartak, Philippe I. H. Bastiaens -- Endocytosis in the Spatial Control of Polarised Cell Functions / Giorgio Scita, Andrea Disanza, Emanuela Frittoli -- Aberrant Vesicular Trafficking Contributes to Altered Polarity and Metabolism in Cancer / Shreya Mitra, Gordon B. Mills -- Endocytosis and the Regulation of Cell Signaling, Cell Adhesion, and Epithelial to Mesenchymal Transition in Cancer / Crislyn D'Souza-Schorey, Guangpu Li -- Efficient Enhancement of Signaling Capacity: Signaling Endosomes / Iwona Pilecka, Marta Miaczynska -- Nuclear Functions and Trafficking of Receptor Tyrosine Kinases / Ying-Nai Wang, Jennifer L. Hsu, Mien-Chie Hung -- Efficient Enhancement of Signalling Capacity: The Ubiquitin System / Daniela Hoeller, Ivan Dikic -- Molecular Mechanism of Ubiquitin-Dependent Traffic / Elena Maspero, Hans-Peter Wollscheid, Simona Polo -- Cbl as a Master Regulator of Receptor Tyrosine Kinase Trafficking / Ke Ma, Stephen C. Kales, Marion M. Nau, Stanley Lipkowitz -- Regulation of Endocytic Trafficking and Signalling by Deubiquitylating Enzymes / Han Liu, Sylvie Urbé, Michael J. Clague -- RTKs as Models for Trafficking Regulation: c-Met/HGF Receptor-c-Met Signalling in Cancer--Location Counts / Carine Joffre, Rachel Barrow, Ludovic Ménard -- Regulation of Epidermal Growth Factor Receptor Signaling by Endocytosis in Normal and Malignant Cells / Sergio Anastasi, Stefano Alemà, Oreste Segatto MD -- Stress-Driven Endocytosis of Tyrosine-Phosphorylated EGFR Leads to Tumorigenesis: The Critical Role of Oxidative Stress / Tzipora Goldkorn, Simone Filosto, Samuel Chung -- Internalisation, Endosomal Trafficking and Recycling of Integrins During Cell Migration and Cancer Invasion / Elena Rainero, Peter V. E. van den Berghe, Jim C. Norman -- Antibody-Mediated Receptor Endocytosis: Harnessing the Cellular Machinery to Combat Cancer / Gabi Tarcic, Yosef Yarden.
  • Part 1. Introduction -- Vitamin D Deficiency: Historical Perspectives / Kumaravel Rajakumar and Michael F. Holick -- Perspective: Evolution of Human Skin Color: How Low Levels of Vitamin D Drove Natural Selection / Scott T. Weiss -- Part 2. Vitamin D Mechanisms of Relevance to Lung Diseases -- Vitamin D and Lung Development in Early Life / Virender K. Rehan and John S. Torday -- Vitamin D and the Innate Immune Response / Aria Vazirnia and Philip T. Liu -- Vitamin D and Regulatory T Cells / Zoë Urry, Sarah Dimeloe and Catherine M. Hawrylowicz -- Dendritic Cell Modulation by the Vitamin D System / Luciano Adorini, Gilles Laverny and Giuseppe Penna -- Vitamin D Modulates Airway Smooth Muscle Function / Audreesh Banerjee and Reynold A. Panettieri Jr. -- Vitamin D: Genetics and Genomic Effects / Kelan G. Tantisira -- Part 3. Disease Associations -- Acute Respiratory Infections / Jonathan M. Mansbach and Carlos A. Camargo Jr. -- The Role of Vitamin D in the Development, Exacerbation, and Severity of Asthma and Allergic Diseases / Augusto A. Litonjua -- Vitamin D and Chronic Obstructive Pulmonary Disease / Wim Janssens, An Lehouck, Marc Decramer and Ghislaine Gayan-Ramirez -- Cystic Fibrosis / Helen M. Buntain and Anne B. Chang -- Tuberculosis / Adrian R. Martineau -- Lung Cancer / David C. Christiani and C. Matthew Kinsey.
  • Vitamin D and health: evolution, biologic functions, and recommended dietary intakes for vitamin D / Michael F. Holick -- Photobiology of vitamin D / Tai C. Chen, Zhiren Lu, and Michael F. Holick -- The functional metabolism and molecular biology of vitamin D action / Lori A. Plum and Hector F. DeLuca -- Metabolism and catabolism of vitamin D, its metabolities and clinically relevant analogs / Glenville Jones -- The molecular biology of the vitamin D receptor / Diane R. Dowd and Paul N. MacDonald -- VDR and RXR subcellular trafficking / Julia Barsony -- Mechanism of action of 1,25-dihydroxyvitamin D₃ on intestinal calcium absorption and renal calcium transport / Dare Ajibade, Bryan S. Benn, and Sylvia Christakos -- Biological and molecular effects of vitamin D on bone / Martin A. Montecino ... [et al.] -- Biological and molecular effects of vitamin D on the kidney / Adriana S. Dusso and Masanori Tokumoto -- Vitamin D and the parathyroids / Justin Silver and Tally Naveh-Many -- Diversity of vitamin D target genes / Carsten Carlberg -- Extrarenal synthesis of 1,25-dihydroxyvitamin D and its health implications / Daniel D. Bikle -- Vitamin D and the innate immunity / Philip T. Liu, Martin Hewison, and John S. Adams -- Vitamin D and colon cancer / Heide S. Cross and Meinrad Peterlik -- Mechanisms of resistance to vitamin D action in human cancer cells / María Jesús Larriba and Alberto Muñoz -- Vitamin D and the brain: a neuropsychiatric perspective / Louise Harvey ... [et al.] -- Vitamin D modulation of adipocyte function / Michael B. Zemel and Xiaocun Sun -- Determinants of vitamin D intake / Mona S. Calvo and Susan J. Whiting -- 25-hydroxyvitamin D assays and their clinical utility / N. Binkley and G. Lensmeyer -- Health disparities and vitamin D / Douglass Bibuld -- Vitamin D deficiency in Canada / David A. Hanley -- Vitamin D deficiency and its health consequences in Northern Europe / Leif Mosekilde -- Vitamin D deficiency and consequences for the health of people in Mediterranean countries / Jose Manuel Quesada-Gomez and Manuel Díaz-Curiel -- Vitamin D deficiency in the Middle East and its health consequences / Ghada El-Hajj Fuleihan -- Vitamin D deficiency in the Middle East and its health consequences for adults / Samer El-Kaissi and Suphia Sherbeeni -- Vitamin D deficiency and its health consequences in Africa / Ann Prentice ... [et al.] -- Vitamin D deficiency and its health consequences in India / R.K. Marwaha and R. Goswami -- Vitamin D deficiency, rickets, and fluorosis in India / C. V. Harinarayan and Shashank R. Joshi -- Vitamin D in Asia / Tim Green and Bernard Venn -- Vitamin D deficiency and its health consequences in New Zealand / Mark J. Bolland and Ian R. Reid -- Toxicity of vitamin D / Reinhold Vieth -- Vitamin D deficiency in pregnancy and lactation and health consequences / Sarah N. Taylor, Carol L. Wagner, and Bruce W. Hollis -- Vitamin D deficiency in children and its health consequences / Amy D. DiVasta, Kristen K. van der Veen, and Catherine M. Gordon -- Dietary calcium deficiency and rickets / John M. Pettifor, Philip R. Fischer, and Tom D. Thacher -- Vitamin D in fracture prevention and muscle function and fall prevention / Heike Bischoff-Ferrari -- Inherited defects of vitamin D metabolism / Marie B. Demay -- Molecular defects in the vitamin D receptor associated with hereditary 1,25-dihydroxyvitamin D-resistant rickets (HVDRR) / Peter J. Malloy and David Feldman -- Receptor-independent vitamin D resistance in subhuman and human primates / John S. Adams ... [et al.] -- 25-hydroxyvitamin D-1α-hydroxylase: studies in mouse models and implications for human disease / David Goltzman -- The health benefits of solar irradiance and vitamin D and the consequences of their deprivation / William B. Grant -- Vitamin D status, solar radiation and cancer prognosis / Johan Moan ... [et al.] -- The epidemiology of vitamin D and cancer risk / Edward Giovannucci -- Vitamin D deficiency and the epidemiology of prostate cancer / Gary G. Schwartz -- Vitamin D for cancer prevention and survival / Edward D. Gorham ... [et al.] -- The anti-cancer effect of vitamin D: what do the randomized trials show? / Joan M. Lappe and Robert P. Heaney -- Sunlight, skin cancer, and vitamin D / Jörg Reichrath -- Vitamin D and the risk of type 1 diabetes / Elina Hyppönen -- Vitamin D and multiple sclerosis / Alberto Ascherio and Kassandra L. Munger -- Vitamin D and type 2 diabetes / Myrto Eliades and Anastassios G. Pittas -- Role of vitamin D for cardiovascular health / Robert Scragg -- Vitamin D, renin, and blood pressure / Yan Chun Li -- Role of vitamin D and vitamin D analogs for bone health and survival in chronic kidney disease / Ishir Bhan, Hector Tamez, and Ravi Thadhani -- Role of vitamin D and ultraviolet radiation in chronic kidney disease / Rolfdieter Krause -- Role of vitamin D in rheumatoid arthritis / Linda A. Merlino -- Vitamin D, respiratory infections, and obstructive airway diseases / Carlos A. Camargo Jr, Adit A. Ginde, and Jonathan M. Mansbach -- Treatment of immunomediated diseases by vitamin D analogs / Luciano Adorini -- Clinical utility of 1,25-dihydroxyvitamin D₃ and its analogues for the treatment of psoriasis / Jörg Reichrath and Michael F. Holick -- Affinity alkylating vitamin D analogs as molecular probes and therapeutic agents / Rahul Ray -- Anti-inflammatory activity of calcitriol that contributes to its therapeutic and chemopreventive effects in prostate cancer / Aruna V. Krishnan and David Feldman.
  • Studied since the 1930s, Vitamin K is a fat-soluble vitamin available primarily in vegetable sources. This book provides a summary of the scientific evidence regarding Vitamin K's health-promoting activities. It covers the chemistry of Vitamin K, deficiency signs and nutritional assessment, metabolism and biochemistry, and pharmacology.
  • Chapter 1. Retinoic acid : metabolism, developmental functions, and evolution / Joô E. Carvalho and Michael Schubert -- chapter 2. Serum retinol-binding protein, obesity, and insulin resistance / Pangala V. Bhat and Daniel-Constantin Manolescu -- chapter 3. Retinoic acid and immunity / Yoshishige Miyabe, Chie Miyabe, and Toshihiro Nanki -- chapter 4. Vitamin D3 Up-regulated Protein 1 (VDUP1) and the immune system / Hyun Woo Suh ... [et al.] -- chapter 5. Rapid pre-genomic responses of vitamin D / Tremaine M. Sterling ... [et al.] -- chapter 6. The role of vitamin D in infectious processes / Russell W. Chesney -- chapter 7. Vitamin D, vitamin D binding protein, and cardiovascular disease / Diane Berry and Elina Hyppn̲en -- chapter 8. Vitamins E and C : effects on matrix components in the vascular system / Jean-Marc Zingg, Mohsen Meydani, and Angelo Azzi -- chapter 9. Vitamin K and vascular calcification / Chandrasekar Palaniswamy ... [et al.] -- chapter 10. Therapeutic effects of pyridoxamine and benfotiamine / Shyamala Dakshinamurti and Krishnamurti Dakshinamurti -- chapter 11. Multifaceted therapeutic potential of vitamin B6 / Shyamala Dakshinamurti and Krishnamurti Dakshinamurti -- chapter 12. Non-prosthetic group functions of biotin / Krishnamurti Dakshinamurti -- chapter 13. Mechanisms of gene transcriptional regulation through biotin and biotin-binding proteins in mammals / Janos Zempleni ... [et al.] -- chapter 14. Folate receptor-mediated therapeutics : folate receptor- mediated particle systems for drug and gene delivery in cancer therapy / Yoshie Maitani -- chapter 15. Vitamin B12 derivatives and preferential targeting of tumors / Evelyne Furger and Eliane Fischer -- chapter 16. Ascorbic acid : binding proteins and pathophysiology / Fryad Rahman and Michel Fontš.
  • Water and the cell 2006, Springer
  • Biotin: biochemical, physiological and clinical aspects -- Niacin status and genomic instability in bone marrow cells: mechanisms favoring the progression of leukemogenesis -- Niacin: vitamin and antidyslipidemic drug -- Beyond the antioxidant: the double life of vitamin C -- Vitamin C in sepsis -- Vitamin C transport and its role in the cental nervous system -- Genetic aspects of folate metabolism -- Enzymatic and non-enzymatic antioxidative effects of folic acid and its reduced derivates -- Folate-linked drugs for the treatment of cancer and inflammatory diseases -- Folate in skin cancer prevention -- Thiamin(e): the spark of life -- Riboflavin in development and cell fate -- Vitamins B6 and cancer -- Vitamin B6 and cardiovascular disease -- Vitamin B6: beyond coenzyme functions -- Cobalamin deficiency -- Biochemistry of B12-cofactors in human metabolism -- Physiological and molecular aspects of cobalamin transport.
  • Wnt signaling 2008, Springer Protocols
    v. 1. Pathway methods and mammalian models -- v. 2. Pathway models.
  • The discovery of zinc fingers and their practical applications in gene regulation : a personal account / Aaron Klug -- C2H2 zinc fingers as DNA binding domains / Shiro Iuchi -- TFIIIA : a sophisticated zinc finger protein / Raymond S. Brown and Jane Flint -- GAGA : structural basis for single Cys2His2 zinc finger-DNA interaction / G. Marius Clore and James G. Omichinski -- The DNA-binding domain of GATA transcription factors : a prototypical Type IV Cys2-Cys2 zinc finger / Angela M. Gronenborn -- MutM : single C2C2 zinc finger-DNA interaction / Ryoji Masui, Noriko Nakagawa, and Seiki Kuramitsu -- Homing endonuclease I-TevI : an atypical zinc finger with a novel function / Patrick Van Roey, Marlene Belfort, and Victoria Derbyshire -- Zinc finger interactions with metals and other small molecules / Jay S. Hanas, Jason L. Larabee, and James R. Hocker -- Synthetic zinc finger transcription factors / Nicoletta Corbi, Valentina Libri, and Claudio Passananti -- TFIIA and p43 : binding to 5S ribosomal RNA / Paul J. Romaniuk -- RNA binding by single zinc fingers / Martyn K. Darby -- Wig-1, a p53-induced zinc finger protein that binds double stranded RNA / Cristina Mendez-Vidal ... [et al.] -- Tandem CCCH zinc finger proteins in mRNA binding / Perry J. Blackshear, Ruth S. Phillips, and Wi S. Lai -- Ribosomal zinc finger proteins : the structure and the function of yeast YL37a / John Dresios, Yuen-Ling Chan, and Ira G. Wool -- LIM domain and its binding to target proteins / Algirdas Velyvis and Jun Qin -- Ring finger-B box-coiled coil (RBCC) proteins as ubiquitin ligase in the control of protein degradation and gene regulation / Kazuhiro Ikeda, Satoshi Inoue, and Masami Muramatsu -- Structure and function of the CBP/p300 TAZ domains / Roberto N. De Guzman ... [et al.] -- A zinc ribbon motif is essential for the formation of functional tetrameric protein kinase CK2 / Odile Filhol, Maria Jose Benitez, and Claude Cochet -- The FYVE finger : a phosphoinositide binding domain / Harald Stenmark -- The BTB domain zinc finger proteins / Gilbert G. Prive ... [et al.] -- KRAB zinc finger proteins : a family of repressors mediating heterochromatin-associated gene silencing / Shiro Iuchi -- The superfamily of SCAN domain containing zinc finger transcription factors / Tucker Collins and Tara L. Sander -- SP1 and Huntington's disease / Dimitri Krainc -- The role of WT1 in development and disease / Sean Bong Lee, Hongjie Li, and Ho-Shik Kim -- Yin yang 1 / Huifei Liu and Yang Shi -- The multiple cellular functions of TFIIIA / Natalie Kuldell -- The role of the Ikaros gene family in lymphocyte development / Pablo Gomez-del Arco ... [et al.] -- Basonuclin : a zinc finger protein of epithelial cells and reproductive germ cells / Howard Green and Hung Tseng -- ZAS zinc finger proteins : the other kB-binding protein family / Carl E. Allen and Lai-Chu Wu -- Role of GATA factors in development / Marc Haenlin and Lucas Waltzer -- The androgen receptor and spinal and bulbar muscular atrophy / F. Piccioni, C.J. Sumner, and Kenneth H. Fischbeck -- The role of XPA in DNA repair / Takahisa Ikegami and Masahiro Shirakawa -- MOF, an acetyl transferase involved in dosage compensation in drosophila, uses a CCHS finger for substrate recognition / Asifa Akhtar and Peter B. Becker -- MDM2 : ring finger protein and regulator of p53 / Liqing Wu and Carl G. Maki -- The zip family of zinc transporters / David J. Eide -- Apoptosis by zinc deficiency / Kirsteen H. Maclean.
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