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Biochemistry

  • 2014 Springer Protocols
    edited by Daniel Raftery, University of Washington and Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
    Overview of mass spectrometry-based metabolomics : opportunities and challenges -- Global metabolic profiling using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry -- LC-MS profiling to link metabolic and phenotypic diversity in plant mapping populations -- Mitochondrial metabolomics using high-resolution Fourier-transform mass spectrometry -- Sample preparation methods for LC-MS-based global aqueous metabolite profiling -- Methods of discovery-based and targeted metabolite analysis by comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry detection -- Analysis of mouse liver metabolites by GC x GC-TOF MS -- Metabolite fingerprinting by capillary electrophoresis-mass spectrometry -- Quantitative metabolomic profiling using dansylation isotope labeling and liquid chromatography mass spectrometry -- Quantitative analysis of amino and organic acids by methyl chloroformate derivatization and GC-MS/MS analysis -- Stable isotope-labeled tracers for metabolic pathway elucidation by GC-MS and FT-MS -- Multiplexed, quantitative, and targeted metabolite profiling by LC-MS/MRM -- Multidimensional mass spectrometry-based shotgun lipidomics -- Comprehensive quantitative determination of PUFA-related bioactive lipids for functional lipidomics using high-resolution mass spectrometry -- Ultra-performance liquid chromatography-mass spectrometry targeted profiling of bile acids : application to serum, liver tissue, and cultured cells of different species -- Analysis of volatile organic compounds in exhaled breath by gas chromatography-mass spectrometry combined with chemometric analysis -- Headspace SPME-GC-MS metabolomics analysis of urinary volatile organic compounds (VOCs) -- Metabolite profiling by direct analysis in real-time mass spectrometry -- Analysis of dried blood spots using DESI mass spectrometry -- DESI-MS imaging of lipids and metabolites from biological samples -- Metabolic imaging using nanostructure-initiator mass spectrometry (NIMS) -- Statistical analysis and modeling of mass spectrometry-based metabolomics data.
  • 2013 Springer Protocols
    edited by Jennifer J. Kohler, Steven M. Patrie.
    Introduction to glycosylation and mass spectrometry / Steven M. Patrie, Michael J. Roth, and Jennifer J. Kohler -- PART I ENRICHMENT AND ISOLATION METHODS -- Tandem lectin weak affinity chromatography for glycoprotein enrichment / Zhi Yuan Ma, Yuliya Skorobogatko, and Keith Vosseller -- CSC technology: Selective labeling of glycoproteins by mild oxidation to phenotype cells / Andreas Hofmann, Damaris Bausch-Fluck, and Bernd Wollscheid -- Use of boronic acid nanoparticles in glycoprotein enrichment / Yawei Xu, Lijuan Zhang, and Haojie Lu -- Incorporation of unnatural sugars for the identification of glycoproteins / Balyn W. Zaro, Howard C. Hang, and Matthew R. Pratt -- Characterization of membrane-associated glycoproteins using lectin affinity chromatography and mass spectrometry / Yashu Liu, Jintang He, and David M. Lubman -- Sialic acid capture-and-release and LC-MS(n) analysis of glycopeptides / Jonas Nilsson and Göran Larson -- PART II SAMPLE PREPARATION -- In-solution digestion of glycoproteins for glycopeptide-based mass analysis / Eden P. Go, Kathryn R. Rebecchi, and Heather Desaire -- Nano-HPLC-MS of glycopeptides obtained after nonspecific proteolysis / Gerhild Zauner, Carolien A.M. Koeleman, André M. Deelder, and Manfred Wuhrer -- PART III SEPARATION METHODS -- Glycopeptide enrichment for MALDI-TOF mass spectrometry analysis by hydrophilic interaction liquid chromatography solid phase extraction (HILIC SPE) / Pia Hønnerup Jensen, Simon Mysling, Peter Højrup, and Ole Nørregaard Jensen -- Separation and identification of glycoforms by capillary electrophoresis with electrospray ionization mass spectrometric detection / Alina D. Zamfir, Corina Flangea, Alina Serb, Ana-Maria Zagrean, Andreas M. Rizzi, and Eugen Sisu -- Structural separations by ion mobility-MS for glycomics and glycoproteomics / Larissa S. Fenn and John A. McLean -- PART IV QUANTITATION METHODS -- Quantitative analysis of glycoprotein glycans / Ron Orlando -- Stable isotope labeling of N-glycosylated peptides by enzymatic deglycosylation for mass spectrometry-based glycoproteomics / Hiroyuki Kaji and Toshiaki Isobe -- Approaches for site mapping and quantification of o-linked glycopeptides / Peng Zhao, Stephanie H. Stalnaker, and Lance Wells -- Glycan profiling: Label-free analysis of glycoproteins / Yoshinao Wada -- PART V COMPUTATIONAL TOOLS -- Introduction to informatics in glycoprotein analysis / Kiyoko F. Aoki-Kinoshita -- Software tools for glycan profiling / Chuan-Yih Yu, Anoop Mayampurath, and Haixu Tang -- PART VI CASE STUDIES IN MASS SPECTROMETRY OF GLYCOPROTEINS -- Quantitative characterization of glycoproteins in neurodegenerative disorders using iTRAQ / Min Shi, Hyejin Hwang, and Jing Zhang -- Quantitative proteomic analysis of N-linked glycoproteins in human tear fluid / Lei Zhou and Roger W. Beuerman -- Elucidation of N-glycosites within human plasma glycoproteins for cancer biomarker discovery / Penelope Drake, Birgit Schilling, Brad Gibson, and Susan Fisher -- Characterizing the glycosylation state of therapeutic recombinant glycoproteins. / Nicole Samuels, David Kates, Jun Liu, and Joanne Severs.
  • 2009 Springer Protocols
    edited by Mary S. Lipton and Ljiljana Paša-Tolić.
    Generation and analysis of multidimensional protein identification technology datasets / Selene K. Swanson, Laurence Florens, and Michael P. Washburn -- Quantitative peptide and protein profiling by mass spectrometry / Alexander Schmidt, Birgit Bisle, and Thomas Kislinger -- Label-free relative quantitation of prokaryotic proteomes using the accurate mass and time tag approach / Kim K. Hixson -- Classical proteomics: two-dimensional electrophoresis/MALDI mass spectrometry / Ursula Zimny-Arndt ... [et al.] -- The use of difference in-gel electrophoresis for quantitation of protein expression / Rajat Sapra -- Liquid-chromatography-mass spectrometry of thylakoid membrane proteins / Christian G. Huber ... [et al.] -- High accuracy mass spectrometry in large-scale analysis of protein phosphorylation / Jesper V. Olsen and Boris Macek -- Manual validation of peptide sequence and sites of tyrosine phosphorylation from MS/MS spectra / Amy M. Nichols and Forest M. White -- Assigning glycosylation sites and microheterogeneities in glycoproteins by liquid chromatography/tandem mass spectrometry / Yehia Mechref, Milan Madera, and Milos V. Novotny -- Structure analysis of N -glycoproteins / Stefanie Henning, Jasna Peter-Katalinicʹ, and Gottfried Pohlentz -- Capillary zone electrophoresis-mass spectrometry for the characterization of isoforms of intact glycoproteins / Christian Neusüss, and Matthias Pelzing -- Top-down proteomics on a high-field fourier transform ion cyclotron resonance mass spectrometer / Séverine A. Ouvry-Patat ... [et al.] -- Capillary isoelectric focusing/reversed phase liquid chromatography/mass spectrometry / Cheng S. Lee and Brian M. Balgley -- Integrating accelerated tryptic digestion into proteomics workflows / Gordon W. Slysz and David C. Schriemer -- Hydrogen/deuterium exchange mass spectrometry / Xuguang Yan and Claudia S. Maier -- Mass spectrometry detection and characterization of noncovalent protein complexes / Sheng Yin and Joseph A. Loos -- Chemical cross-linking for protein-protein interaction studies / Xiaoting Tang and James E. Bruce -- Tissue analysis with high-resolution imaging mass spectrometry / A.F. Maarten Altelaar and Ron M.A. Heeren -- Proteomic global profiling for cancer biomarker discovery / Vitor Faca, Hong Wang, and Samir Hanash -- Analysis of protein glycosylation and phosphorylation using liquid phase separation, protein microarray technology, and mass spectrometry / Jia Zhao ... [et al.] -- Transthyretin mass determination for detection of transthyretin familial amyloid / John F. O'Brien and H. Robert Bergen III -- Characterization of microorganisms by MALDI mass spectrometry / Catherine E. Petersen, Nancy B. Valentine, and Karen L. Wahl -- Mass spectrometric characterization of neuropeptides / Stephanie S. DeKeyser, James A. Dowell, and Lingjun Li -- Peptide and protein ion/ion reactions in electrodynamic ion traps: tools and methods / Scott A. McLuckey -- Electron capture dissociation LC/MS/MS for bottom-up proteomics / Roman A. Zubarev -- Two-dimensional ion mobility analyses of proteins and peptides / Alexandre A. Shvartsburg, Keqi Tang, and Richard D. Smith -- Proteomics for validation of automated gene model predictions / Kemin Zhou ... [et al.] -- Support vector machines for improved peptide identification from tandem mass spectrometry database search / Bobbie-Jo M. Webb-Robertson.
  • 2012 Springer
    Satya Prakash Gupta, editor.
    Matrix Metalloproteinases / Viola Vargová, Marek Pytliak and Viola Mechírová -- Specificity of Binding with Matrix Metalloproteinases / Satya P. Gupta and Vaishali M. Patil -- The Gelatinases and Their Inhibitors: The Structure-Activity Relationships / Snehasikta Swarnakar, Amartya Mishra and Susri Ray Chaudhuri (Guha) -- Advances in Studies on Collagenase Inhibitors / Mange Ram Yadav, Prashant Revan Murumkar and Vishal Prakash Zambre -- Hydroxamic Acids as Matrix Metalloproteinase Inhibitors / Rajeshwar P. Verma -- Quantitative Structure-Activity Relationship Studies on Sulfonamide-Based MMP Inhibitors / Vaishali M. Patil and Satya P. Gupta -- Matrix Metalloproteinase Inhibitors as Investigative Tools in the Pathogenesis and Management of Vascular Disease / Mina M. Benjamin and Raouf A. Khalil.
  • 2008 Springer
    Vincent Lagente, Elisabeth Boichot, editors.
    Also available: Print – 2008
  • 2014 Springer
    Carsten Carlberg, Ferdinand Molnár.
    This textbook aims to describe the fascinating area of eukaryotic gene regulation for graduate students in all areas of the biomedical sciences. Gene expression is essential in shaping the various phenotypes of cells and tissues and as such, regulation of expression is a fundamental aspect of nearly all processes in physiology, both in healthy and in diseased states. This pivotal role for the regulation of gene expression makes this textbook essential reading from students of all the biomedical sciences in order to be better prepared for their specialized disciplines. A complete understanding of transcription factors and the processes that alter their activity is a major goal of modern life science research. The availability of the whole human genome sequence (and that of other eukaryotic genomes) and the consequent development of next-generation sequencing technologies have significantly changed nearly all areas of the biological sciences. For example, the genome-wide location of histone modifications and transcription factor biding sites, such as provided by the ENCODE consortium, has greatly improved our understanding of gene regulation. Therefore, the focus of this book is the description of the post-genome understanding of gene regulation. The purpose of this book is to provide, in a condensed form, an overview on the present understanding of the mechanisms of gene regulation.
  • 2008 Springer
    Andre Kamkin, Irina Kiseleva, editors ; forewords by Max J. Lab and Jürgen Hescheler.
  • 2011 Springer
    Andre Kamkin, Irina Kiseleva, editors ; foreward by Holger Scholz.
  • 2014 ClinicalKey
    John W. Baynes, Marek H. Dominiczak.
    Brought to you in a thorough yet accessible manner, the new edition of Medical Biochemistry gives access to all of the latest information on basic and clinically focused genetic and molecular biology. Featuring a team of contributors that includes investigators involved in cutting-edge research as well as experienced clinicians, this updated medical textbook offers a unique combination of both research and practice that's ideal for today's problem-based integrated courses. Relate biochemistry to everyday practice with the help of Clinical Boxes integrated into the text, and access in-depth coverage of important topics - including recent research in biochemistry - through Advanced Concept Boxes.Test your knowledge and improve retention with Active Learning Boxes at the conclusion of each chapter, and quickly review the most common lab tests performed with convenient Clinical Test Boxes.Effectively study the most updated information in biochemistry with the help of a dynamic, full-color design.Better understand the relationship between science and clinical practice with material organized by organ rather than system.
  • 2002 ScienceDirect
    N.V. Bhagavan.
    Also available: Print – 2002
  • 2010 Springer
    edited by Anna Catania.
    Structure-activity relationships (SAR) of melanocortin and agouti-related (AGRP) peptides / Anamika Singh, Erica M. Haslach and Carrie Haskell-Luevano -- Melanocortin signalling mechanisms / Paula C. Eves and John W. Haycock -- Distribution and function of melanocortin receptors within the brain / Kathleen G. Mountjoy -- Drugs, exercise, and the melanocortin-4 receptor: different means, same ends: treating obesity / Jay W. Schaub, Erin B. Bruce, and Carrie Haskell-Luevano -- Melanocortins in brain inflammation: the role of melanocortin receptor subtypes / Ruta Muceniece and Maija Dambrova -- Melanocortins and the cholinergic anti-inflammatory pathway / Daniela Giuliani ... [et al.] -- Melanocortin control of cell trafficking in vascular inflammation / Hetal B. Patel ... [et al.] -- Terminal signal: anti-inflammatory effects of α-melanocyte-stimulating hormone related peptides beyond the pharmacophore / Thomas Brzoska ... [et al.] -- Protective effects of melanocortins in systemic host reactions / Stefano Gatti ... [et al.] -- Development of α-melanocortin analogs for melanoma prevention and targeting / Zalfa A. Abdel-Malek -- MSH radiopeptides for targeting melanoma metastases / Alex N. Eberle ... [et al.] -- Applications of the role of α-MSH in ocular immune privilege / Andrew W. Taylor and Darren Lee.
    Also available: Print – 2010
  • 2015 Springer
    E. Anibal Disalvo, editor.
    1. Membrane hydration: a hint to a new model for biomembranes -- 2. Use of x-ray and neutron scattering methods with volume measurements to determine lipid bilayer structure and number of water molecules/lipid -- 3. Water and lipid bilayers -- 4. Hydration forces between lipid bilayers: a theoretical overview and a look on methods exploring dehydration -- 5. Monitoring membrane hydration with 2-(dimethylamino)-6-acylnaphtalenes fluorescent probes -- 6. Long-range lipid-water interaction as observed by ATR-FTIR spectroscopy -- 7. Hydration and nanoconfined water: insights from computer simulations -- 8. Aquaphotomics: near infrared spectoscopy and water states in biological systems -- 9. Hydration in lipid monolayers: correlation of water activity and surface pressure -- 10. Water at biological phase boundaries: its role in interfacial activation of enzymes and metabolic pathways -- 11. Anhydrobiosis: an unsolved problem with applications in human welfare -- Concluding remarks -- Index.
  • 2003. ScienceDirect
    volume editor, Mark P. Mattson.
  • 2002 ScienceDirect
    edited by Carola Hunte and Gebhard von Jagow and Hermann Schagger.
  • 2012 Springer Protocols
    edited by Nagarajan Vaidehi, Judith Klein-Seetharaman.
    Crystallization of membrane proteins in bicelles -- Vapor diffusion-controlled meso crystallization of membrane proteins -- Solution NMR studies of integral polytopic alpha-helical membrane proteins: The structure determination of the seven-helix transmembrane receptor sensory rhodopsin ii, psrii -- Use of NMR saturation transfer difference spectroscopy to study ligand binding to membrane proteins -- How to investigate interactions between membrane proteins and ligands by solid-state NMR -- Identifying and measuring transmembrane helix-helix interactions by FRET -- Studying substrate binding to reconstituted secondary transporters by attenuated total reflection infrared difference spectroscopy -- UV-visible and infrared methods for investigating lipid-rhodopsin membrane interactions -- Proteomic characterization of integral membrane proteins using thermostatted liquid chromatography coupled with tandem mass spectrometry -- LITiCon: A discrete conformational sampling computational method for mapping various functionally selective conformational states of transmembrane helical proteins -- Homology model-assisted elucidation of binding sites in GPCRs -- Comparative modeling of lipid receptors -- Quantification of structural distortions in the transmembrane helices of GPCRs -- Structure prediction of g protein-coupled receptors and their ensemble of functionally important conformations -- Target based virtual screening by docking into automatically generated GPCR models -- Predicting the biological activities through QSAR analysis and docking-based scoring -- Identification of motions in membrane proteins by elastic network models and their experimental validation -- Modeling the structural communication in supramolecular complexes involving GPCRs -- Exploring substrate diffusion in channels using biased molecular dynamics simulations.
  • 2013 Springer Protocols
    edited by Giovanna Ghirlanda, Alessandro Senes.
    Use of Thiol-Disulfide Exchange Method to Study Transmembrane Peptide Association in Membrane Environments -- Measurement of Transmembrane Peptide Interactions in Liposomes Using Fr ster Resonance Energy Transfer (FRET) -- Measuring Transmembrane Helix Interaction Strengths in Lipid Bilayers Using Steric Trapping -- Genetics Systems for Monitoring Interactions of Transmembrane Domains in Bacterial Membranes -- Analyzing the Effects of Hydrophobic Mismatch on Transmembrane -Helices Using Tryptophan Fluorescence Spectroscopy -- Folding Alpha Helical Membrane Proteins into Liposomes In Vitro and Determination of Secondary Structure -- Solvation Models and Computational Prediction of Orientations of Peptides and Proteins in Membranes -- Membrane Protein Structure Determination: Back to the Membrane -- On the Role of NMR Spectroscopy for Characterization of Antimicrobial Peptides -- Prediction and Design of Outer Membrane Protein-Protein Interactions -- Design of Transmembrane Peptides: Coping with Sticky Situations -- Engineering and Utilization of Reporter Cell Lines for Cell-Based Assays of Transmembrane Receptors -- Fluorination in the Design of Membrane Protein Assemblies.
  • 2015 ScienceDirect
    edited by Arun K. Shukla.
  • 2015 ScienceDirect
    edited by Arun K. Shukla.
    Membrane Proteins - Production and Function Characterization a volume of Methods in Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.
  • 2010 Springer
    Jason X.-J. Yuan, Jeremy P.T. Ward, editors.
    The role of ion channels in hypoxic pulmonary vasoconstriction / E. Kenneth Weir ... [et al.] -- Two-pore domain K channels and their role in chemoreception / Keith J. Buckler -- Intricate interaction between store-operated calcium entry and calcium-activated chloride channels in pulmonary artery smooth muscle cells / Abigail S. Forrest ... [et al.] -- The role of intracellular ion channels in regulating cytoplasmic calciumin pulmonary arterial mmooth [i.e. smooth] muscle : which store and where? / A. Mark Evans -- Ca2 oscillations regulate contraction of intrapulmonary smooth muscle cells / Michael J. Sanderson, Yan Bai, and Jose Perez-Zoghbi -- Introduction to TRP channels : structure, function, and regulation / Michael Y. Song and Jason S.-J. Yuan -- Physiological functions of transient receptor potential channels in pulmonary arterial smooth muscle cells / Xiao-Ru Yang, Mo-Jun Lin, and James S.K. Sham -- The contribution of TRPC1 and STIM1 to capacitative Ca2 entry in pulmonary artery / Lih Chyuan Ng, Judith A. Airey, and Joseph R. Hume -- Store-operated calcium entry channels in pulmonary endothelium : the emerging story of TRPCS and Orai1 / Donna L. Cioffi, Christina Barry, and Troy Stevens -- TRPM2 channel regulates endothelial barrier function / Claudie M. Hecquet, Gias U. Ahmmed, and Asrar B. Malik -- A proposed mitochondrial-metabolic mechanism for initiation and maintenance of pulmonary arterial hypertension in fawn-hooded rats : the Warburg model of pulmonary arterial hypertension / Jalees Rehman and Stephen L. Archer -- The role of classical transient receptor potential channels in the regulation of hypoxic pulmonary vasoconstriction / B. Fuchs ... [et al.] -- Developmental regulation of oxygen sensing and ion channels in the pulmonary vasculature / David N. Cornfield -- Hypoxic regulation of ion channels and transporters in pulmonary vascular smooth muscle / Larissa A. Shimoda -- CLC-3 chloride channels in the pulmonary vasculature / Joseph R. Hume ... [et al.] -- Role of bone morphogenetic protein receptors in the development of pulmonary arterial hypertension / Nicholas W. Morrell -- Cross talk between Smad, MAPK, and actin in the etiology of pulmonary arterial hypertension / James West -- Notch signaling in pulmonary hypertension / Patricia A. Thistlethwaite, Xiaodong Li, and Xiaoxue Zhang -- Rho kinase-mediated vasoconstriction in pulmonary hypertension / Ivan F. McMurtry ... [et al.] -- The serotonin hypothesis of pulmonary hypertension revisited / Margaret R. MacLean and Yvonne Dempsie -- Impaired vascular endothelial growth factor signaling in the pathogenesis of neonatal pulmonary vascular disease / Steven H. Abman -- Mitochondrial regulation of oxygen sensing / Navdeep S. Chandel -- Reactive oxygen species and RhoA signaling in vascular smooth muscle : role in chronic hypoxia-induced pulmonary hypertension / Thomas C. Resta, Brad R.S. Broughton, and Nikki L. Jernigan -- Polyamine regulatory pathways as pharmacologic targets in pulmonary arterial hypertension / Mark N. Gillespie and Jack W. Olson -- 5-HT receptors and Kv channel internalization / Angel Cogolludo and Francisco Perez-Vizcaino -- KCNQ potassium channels : new targets for pulmonary vasodilator drugs? / Alison M. Gurney, Shreena Joshi, and Boris Manoury -- Receptor tyrosine kinase inhibitors in rodent pulmonary hypertension / Liliana Moreno-Vinasco and Joe G.N. Garcia -- PDGF receptor and its antagonists : role in treatment of PAH / Friedrich Grimminger and Ralph Theo Schermuly -- PPAR[gamma] and the pathobiology of pulmonary arterial hypertension / Marlene Rabinovitch -- Targeting TASK-1 channels as a therapeutic approach / Andrea Olschewski -- Pharmacological targets for pulmonary vascular disease : vasodilation versus anti-remodelling / Matthew Thomas.
    Also available: Print – 2010
  • 2015 ScienceDirect
    edited by Christian M. Metallo.
  • 2001 ScienceDirect
    Hiroyasu Aizawa.
    Acid amides -- Amidines, guanidines, and hydrazines -- Amino acids: phosphinic acids -- Anilines and nitrobenzenes -- Aryloxy acids -- Biphenyl ethers -- Carbamates -- Dithio- and thiolcarbamates -- Halogenated aliphatics -- Halogenated aromatics -- Five-membered heterocycles -- Five-membered heterocycles (fused) -- Six-/more membered heterocycles -- Six-/more membered heterocycles (fused) -- Imides -- Natural products -- Organophosphorous compounds -- Oximes -- Phenylureas and related compounds -- Phosphono amino acids and related compounds -- Pyrethroids -- Sulfonylureas -- Triazines and related compounds -- Miscellaneous.
  • 2011 Springer Protocols
    edited by Thomas O. Metz.
    Origins of metabolic profiling / Arthur B. Robinson and Noah E. Robinson -- Amino acid profiling for the diagnosis of inborn errors of metabolism / Monique Piraud ... [et al.] -- Acylcarnitines : analysis in plasma and whole blood using tandem mass spectrometry / David S. Millington and Robert D. Stevens -- Analysis of organic acids and acylglycines for the diagnosis of related inborn errors or metabolism by GC- and HPLC-MS / Giancarlo la Marca and Cristiano Rizzo -- HPLC analysis for the clinical-biochemical diagnosis of inborn errors of metabolism of purines and pyrimidines / Giuseppe Lazzarino ... [et al.] -- Bile acid analysis in various biological samples using ultra performance liquid chromatography/electrospray ionization-mass spectrometry (UPLC/ESI-MS) / Masahito Hagio, Megumi Matsumoto, and Satoshi Ishizuka -- Analysis of glycolytic intermediates with ion chromatography- and gas chromatography-mass spectrometry / Jan C. van Dam, Cor Ras, and Angela ten Pierick -- Analysis of the citric acid cycle intermediates using gas chromatography-mass spectrometry / Rajan S. Kombu, Henri Brunengraber, and Michelle A. Puchowicz -- Quantification of pentose phosphate pathway (PPP) metabolites by liquid chromatography-mass spectrometry (LC-MS) / Amber Jannasch, Miroslav Sedlak, and Jiri Adamec -- High-performance liquid chromatography-mass spectrometry (HPLC-MS)-based drug metabolite profiling / Ian D. Wilson -- Gas chromatography-mass spectrometry (GC-MS)-based metabolomics / Antonia Garcia and Coral Barbas -- Use of two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOF-MS) for metabolomic analysis of polar metabolites / Kimberly Ralston-Hooper ... [et al.] -- LC-MS-based metabolomics / Sunil Bajad and Vladimir Shulaev -- Capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS)-based metabolomics / Philip Britz-McKibbin -- Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics for studies of body fluids and tissues / Heli Nygren ... [et al.] -- Electrospray ionization tandem mass spectrometry (ESI-MS/MS)- based shotgun lipidomics / Giorgis Isaac -- Processing and analysis of GC/LC-MS based metabolomics data / Elizabeth Want and Perrine Masson -- Nuclear magnetic resonance (NMR)-based drug metabolite profiling / Eva M. Lenz -- Nuclear magnetic resonance (NMR)-based metabolomics / Hector C. Keun and Toby J. Athersuch -- Slow magic angle sample spinning : a non- or minimally invasive method for high-resolution ¹H nuclear magnetic resonance (NMR) metabolic profiling / Jian Zhi Hu -- Processing and modeling of nuclear magnetic resonance (NMR) metabolic profiles / Timothy M. D. Ebbels, John C. Lindon, and Muireann Coen.
  • 2015 Springer Protocols
    edited by Jacob T. Bjerrum.
    Metabonomics : analytical techniques and associated chemometrics / Jacob T. Bjerrum -- Sample collection and preparation of biofluids and extracts for NMR spectroscopy / Gwénaëlle Le Gall -- NMR sprectroscopy of biofluids and extracts / Gwénaëlle Le Gall -- High-resolution magic-angle-spinning NMR spectroscopy of intact tissue / Guro F. Giskeødegård, Maria D. Cao, and Tone F. Bathen -- Sample collection and preparation of biofluids and extracts for liquid chromatography-mass spectrometry / Peiyuan Yin [and three others] -- Liquid chromatography-mass spectrometry of biofluids and extracts / Xinjie Zhao [and three others] -- Sample collection and preparation of biofluids and extracts for gas chromatography-mass spectrometry / Abdul-Hamid M. Emwas, Zeyad A. Al-Talla, and Najeh M. Kharbatia -- Gas chromatography-mass spectrometry of biofluids and extracts / Abdul-Hamid M. Emwas [and three others] -- Capillary electrophoresis-mass spectrometry / Masataka Wakayama, Akiyoshi Hirayama, and Tomoyosji Soga -- Preprocessing of raw metabonomic data / Riyas Vettukattil -- Extracting meaningful information from metabonomic data using multivariate statistics / Max Bylesjö -- Combining metabonomics and other -omics data / Mattias Rantalainen -- The strengths and weaknesses of NMR-spectroscopy and mass spectrometry with particular focus on metabolomics research / Abdul-Hamid M. Emwas -- Metabonomics and drug development / Pranov Ramana [and three others] -- Metabonomics and toxicology / Liang Zhao and Thomas Hartung -- Metabonomics and diagnostics / Lucy C. Hicks, Simon J.L. Ralphs, and Horace R.T. Williams -- Metabonomics and systems biology / Vicky De Preter.
  • 2012 Springer
    Wolfgang Linert, Henryk Kozlowski, editors.
    Metal ions in the brain are a necessity as well as a poison. The presence of metal ions in the active sites of biological catalysts or metalloproteins and in the biological functioning of nucleic acids is very well documented and they are required for brain activity. On the other hand, metals are very effective in generating oxidative stress. This effect does not only play a role in immunology but also is the root of practically all neurodegenerative disorders by inducing disease via the death of neurons. Managing metal ions in the brain could therefore be an important strategy in the search for therapeutic agents used in the treatment of neurodegenerative diseases. This new title gives an overview to key topics in the area of metal ions in the brain. It focuses on the role of metal ions in neurological systems by describing their advantageous functions as well as their poisonous features. It is therefore of interest for scientists in biochemistry and biophysics, physiology, toxicology as well as for physicians focused on this topic.
  • ScienceDirect
    edited by James F. Riordan, Bert L. Vallee.
    Also available: Print – 1991
  • ScienceDirect
    edited by James F. Riordan and Bert L. Vallee.
    Also available: Print – 1993
  • ScienceDirect
    edited by James Riordan and Bert Vallee.
    Also available: Print – 1993
  • 2014 Springer Protocols
    edited by Juan C. Fontecilla- Camps, Yvain Nicolet.
    Advances in Research on Metalloproteins -- Purification of O2-Sensitive Metalloproteins -- Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources -- Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins -- Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Metal Centers -- Electrochemistry of Metalloproteins Protein Film Electrochemistry for the Study of E. coli [NiFe]-Hydrogenase-1 -- FTIR Spectroscopy of Metalloproteins -- Characterizing Millisecond Intermediates in Hemoproteins Using Rapid-Freeze-Quench Resonance Raman Spectroscopy -- A Practical Guide for Nuclear Resonance Vibrational Spectroscopy (NRVS) of Biochemical Samples and Model Compounds -- Study of Metalloproteins Using Continuous Wave Electron Paramagnetic Resonance (EPR) -- Mssbauer Spectroscopy -- X-Ray Absorption Spectroscopy of Metalloproteins -- X-Ray Crystallographic Studies of Metalloproteins -- Quantum Mechanical Methods for the Investigation of Metalloproteins and Related Bioinorganic Compounds -- Density Functional Theory Broken Symmetry (DFT-BS) Methodology Applied to Electronic and Magnetic Properties of Bioinorganic Prosthetic Groups.
  • 2011 Springer Protocols
    edited by Craig W. Stevens.
    In silico identification of novel G protein-coupled receptors / Matthew N. Davies, David E. Gloriam, and Darren R. Flower -- Alternative pre-mRNA splicing of G protein-coupled receptors / Ying-Xian Pan, Steven Grinnell, and Gavril W. Pasternak -- Detecting polymorphisms in G protein-coupled receptor genes / Dmitri Proudnikov, Vadim Yuferov, and Mary Jeanne Kreek -- Regulation of the transcription of G protein-coupled receptor genes / Cheol Kyu Hwang [and others] -- Deciphering the evolution of G protein-coupled receptors in vertebrates / Craig W. Stevens -- New approach to visualize endogenously expressed G protein-coupled receptors in tissues and living cells / Gregory Scherrer and Brigitte L. Kieffer -- Posttranslational regulation of G protein-coupled receptors / Yu Qiu and Ping-Yee Law -- Discovering G protein-coupled receptor motifs mediating export from the endoplasmic reticulum / Chunmin Dong and Guangyu Wu -- Identifying G protein-coupled receptor escorts, chaperones, and intracellular tethers regulating receptor density at the cell surface / Stefano Marullo, Liliana Pardo Lopez, and Lamia Achour -- Novel method for determining the kinetics of G protein-coupled receptor plasma membrane expression / Gregory W. Sawyer -- Characterizing the pharmacology of G protein-coupled receptors in transfected cell lines / Kathryn A. Seely and Paul L. Prather -- Novel assay technologies for the discovery of G protein-coupled receptor drugs / Elisa Alvarez-Curto, Richard J. Ward, and Graeme Milligan -- Discovering cell type-specific patterns of G protein-coupled receptor phosphorylation / Kok Choi Kong, Andrew B. Tobin, and Adrian J. Butcher -- Quantifying allosteric modulation of G protein-coupled receptors / Frederick J. Ehlert and Hinako Suga -- Experimental designs for the study of receptor-receptor interactions / Dennis Paul -- Elucidating agonist-selective mechanisms of G protein-coupled receptor desensitization / Chris P. Bailey and Eamonn Kelly -- Detecting the role of arrestins in G protein-coupled receptor regulation / Laura M. Bohn and Patricia H. McDonald -- Characterizing molecular mobility and membrane interactions of G protein-coupled receptors / Vladana Vukojevice, Yu Ming, and Lars Terenius -- Using RNA interference to downregulate G protein-coupled receptors / Philippe Sarret [and others] -- Upregulating G protein-coupled receptors with receptor antagonists / Ellen M. Unterwald.
  • v. 1-, 1955- ScienceDirect
    Abelson, John N.; Colowick, Sidney P.; Kaplan, Nathan Oram; Simon, Melvin I.
  • 2015 Springer Protocols
    edited by Dylan M. Owen.
    Introduction to membrane lipids /Richard M. Epand -- Introduction : membrane properties (good) for life /Marek Cebecauer -- Introduction to fluorescence probing of biological membranes /Alexander P. Demchenko ... [et al.] -- Assembly and use of tethered bilayer lipid membranes (tBLMs) /Charles Cranfield ... [et al.] -- Preparation of detergent-resistant membranes (DRMs) from cultured mammalian cells /Deborah A. Brown -- Isolation of giant plasma membrane vesicles for evaluation of plasma membrane structure and protein partitioning /K. R. Levental and I. Levental -- Asymmetric giant lipid vesicle fabrication /Peichi C. Hu and Noah Malmstadt -- Cholesterol depletion using Methyl-[Beta]-cyclodextrin /Saleemulla Mahammad and Ingela Parmryd -- Comparative LC-MS based profiling approach to analyze lipid composition in tissue culture systems /G. Ekin Atilla-Gokcumen and Ulrike S. Eggert -- Imaging membrane order using environmentally sensitive fluorophores /G.W. Ashdown and Dylan M. Owen -- 3D super-resolution imaging by localization microscopy /Astrid Magenau and Katharina Gaus -- Electron microscopy methods for studying plasma membranes /Alison J. Beckett and Ian A. Prior -- Measuring cytoskeleton and cellular membrane mechanical properties by atomic force microscopy /Charles Roduit ... [et al.] -- Fluorescence linear dichroism imaging for quantifying membrane order /Richard K. P. Benninger -- Scanning fluorescence correlation spectroscopy on biomembranes /Eduard Hermann, Jonas Ries, and Ana J. García-Sáez -- X-ray diffraction of lipid model membranes /Arwen I. I. Tyler, Robert V. Law, and John M. Seddon -- Solid state NMR of lipid model membranes /Arwen I. I. Tyler ... [et al.] -- Fluorescence recovery after photobleaching (FRAP) : acquisition, analysis, and applications /Michael Carnell, Alex Macmillan, and Renee Whan -- Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells /Ottavia Golfetto, Elizabeth Hinde, and Enrico Gratton -- Cholesterol behavior in asymmetric lipid bilayers : insights from molecular dynamics simulations /Semen O. Yesylevskyy and Alexander P. Demchenko -- Computer simulations of phase separation in lipid bilayers and monolayers /Svetlana Baoukina and D. Peter Tieleman.
  • 2007 Springer Protocols
    edited by Alex M. Dopico.
  • 2011 ScienceDirect
    edited by Amy C. Rosenzweig, Stephen W. Ragsdale.
    1. Techniques for cultivating methanogens / Ralph S. Wolfe -- 2. Genetic methods for methanosarcina species / Nicole Buan, Gargi Kulkarni, and William Metcalf -- 3. Genetic systems for hydrogenotrophic methanogens / Felipe Sarmiento B., John A. Leigh, and William B. Whitman -- 4. Molecular tools for investigating ANME community structure and function / Steven J. Hallam ... [et al.] -- 5. Studying gene regulation in methanogenic archaea / Michael Rother, Christian Sattler, and Tilmann Stock -- 6. Growth of methanogens under defined hydrogen conditions / John A. Leigh -- 7. Preparation of [Fe]-hydrogenase from methanogenic archaea / Seigo Shima, Michael Schick, and Haruka Tamura -- 8. Assay of methylotrophic methyltransferases from methanogenic archaea / Donald J. Ferguson, David G. Longstaff, and Joseph A. Krzycki -- 9. Methyl-coenzyme M reductase from methanothermobacter marburgensis / Evert C. Duin, Divya Prakash, and Charlene Brungess -- 10. Methods for analysis of acetyl-CoA synthase : applications to bacterial and archaeal systems / David A. Grahame -- 11. Acetate kinase and phosphotransacetylase / James G. Ferry -- 12. Sodium ion translocation and ATP synthesis in methanogens / Katharina Schlegel and Volker Müller -- 13. Proton translocation in methanogens / Cornelia Welte and Uwe Deppenmeier -- 14. Measuring isotope fractionation by autotrophic microorganisms and enzymes / Kathleen M. Scott, Gordon Fox, and Peter R. Girguis -- 15. 2-oxoacid metabolism in methanogenic CoM and CoB biosynthesis / David E. Graham -- 16. Biomethanation and its potential / Irini Angelidaki ... [et al.].
    Also available: Print – 2011
  • 2011 ScienceDirect
    edited by Amy C. Rosenzweig, Stephen W. Ragsdale.
    Also available: Print – 2011
  • pt. A-B, 2014. ScienceDirect
    pt. B ScienceDirect
    edited by Ormond A. Macdougald.
  • 2012 Springer
    Bertrand Jordan, editor.
    Microarray technology has made strong progress over the past decade, and there have also been significant changes in application areas, from nucleic acids to proteomics and from research to clinical applications. This book provides a comprehensive overview of microarrays in diagnostics and biomarker development, covering DNA, peptide, protein and tissue arrays. The focus is on entities that are in actual clinical use, or quite close, and on recent developments, such as peptide and aptamer arrays. A further topic is the miniaturisation towards nanoarrays, which is expected to have great potential in clinical applications. Relevant issues of bioinformatics and statistical analysis of array data are discussed in detail, as well as the barriers to the commercialisation of array-based tests and the vexing IP issues involved. Thus, the book should be very useful tor active array users as well as to newcomers seeking to make the best choice between different technologies.
  • 2013 ScienceDirect
    edited by Edward F. DeLong.
    This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers microbial metagenomics, metatranscriptomics, and metaproteomics, and includes chapters on such topics as in-solution FISH for single cell genome preparation, preparation of BAC libraries from marine microbial community DNA, and preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton. Continues the legacy of this premier serial with quality chapters authored by leaders in the field.
  • 2006 Wiley
    [edited by] Ian Humphery-Smith, Michael Hecker.
    "Discover important lessons learned about whole organism biology via microbial proteomics This text provides an exhaustive analysis and presentation of current research in the field of microbial proteomics, with an emphasis on new developments and applications and future directions in research. The editors and authors show how and why the relative simplicity of microbes has made them attractive targets for extensive experimental manipulation in a quest for both improved disease prevention and treatment and an improved understanding of whole organism functional biology. In particular, the text demonstrates how microbial proteomic analyses can aid in drug discovery, including identification of new targets, novel diagnostic markers, and lead optimization. Each chapter is written by one or more leading experts in the field and carefully edited to ensure a consistent and thorough approach throughout. Methods, technologies, and tools associated with the most promising approaches are stressed."--Publisher's website.
    Also available: Print – 2006
  • 2013 Springer Protocols
    edited by Stéphane Marinesco, Nicholas Dale.
    Microelectrode designs for oxidase-based biosensors / Stephane Marinesco and Olivier Frey -- Microelectrode array fabrication and optimization for selective neurochemical detection / Kevin N. Hascup [and others] -- Advantage of microelectrode technologies for measurement in delicate biological environments such as brain tissue / Andrea Jaquins-Gerstl and Adrian C. Michael -- Hexacyanoferrates as mediators for microelectrode biosensors / Faming Tian and Nicholas Dale -- Enzyme immobilization on microelectrode biosensors / Natalia Vasylieva and Stephane Marinesco -- Characterization of polymer-enzyme composite biosensors for brain monitoring in vivo / Robert D. O'Neill -- Monitoring extracellular glutamate in the brain by microdialysis and microsensors / Si Qin [and others] -- Sub-second measurements of glutamate and other neurotransmitter signaling using enzyme-based ceramic microelectrode arrays / Erin R. Hascup [and others] -- Regulation of extracellular concentrations of D-serine in the central nervous system revealed by D-amino acid oxidase microelectrode biosensors / Pierre Pernot, Caroline Maucler, and Stephane Marinesco -- Measurement of purine release with microelectrode biosensors / Nicholas Dale -- Glucose and lactate monitoring across the rat sleep-wake cycle / Raymond Cespuglio, Larissa Netchiporouk, and Nataliya Shram -- Forebrain cholinergic systems and cognition : new insights based on rapid detection of choline spikes using enzyme-based biosensors / Vinay Parikh and Martin Sarter -- Carbon nanotubes-based microelectrode (bio)sensors / Paloma Yanez-Sedeno, Lourdes Agui, and Jose M. Pingarron -- Label-free affinity biosensors based on electrochemical impedance spectroscopy / Nicole Jaffrezic-Renault -- Aptamer-based electrochemical biosensors for the detection of small molecules and plasma proteins / Cassie Ho and Hua-Zhong Yu -- Electrochemiluminescent biosensors : neuroscience applications / Robert J. Forster and Tia E. Keyes -- Molecularly imprinted polymers : promising advanced materials for in vivo sensing / Yi Ge [and others].
  • 2010 Springer
    Zhiguo Wang, Baofeng Yang.
  • 2009. Springer
    Zhiguo Wang.
  • 2007 ScienceDirect
    edited by John J. Rossi, Gregory J. Hannon.
    Also available: Print – 2007
  • 2007 Springer Protocols
    edited by Jun Zhou.
  • 2010 Springer
    edited by Thomas P. Jahn and Gerd P. Bienert.
    Aquaporins : a family of highly regulated multifunctional channels / Charles Hachez and François Chaumont -- Phylogeny of major intrinsic proteins / Jonas Å.H. Danielson and Urban Johanson -- Metalloids, soil chemistry, and the environment / Enzo Lombi and Peter E. Holm -- Arsenic transport in prokaryotes and eukaryotic microbes / Barry P. Rosen and Markus J. Tamøs -- Metalloid transport by aquaglyceroporins : consequences in the treatment of human diseases / Rita Mukhopadhyay and Eric Beitz -- Roles of vertebrate aquaglyceroporins in arsenic transport and detoxification / Zijuan Liu -- Molecular mechanisms of boron transport in plants : involvement of arabidopsis NIP5;1 and NIP6;1 / Kyoko Miwa ... [et al.] -- Silicon transporters in higher plants / Jian Feng Ma -- Major intrinsic proteins and arsenic transport in plants : new players and their potential roles / Gerd P. Bienert and Thomas P. Jahn -- Major intrinsic proteins in biomimetic membranes / Claus Hølix Nielsen.
    Also available: Print – 2010
  • 2006 Springer
    edited by Regina M. Murphy, Amos M. Tsai.
  • 2007 Springer
    edited by Stephen W. Schaffer and M.-Saadeh Suleiman.
    Regulation of mitochondrial respiration in heart muscle / Ilmo Hassinen -- Regulation of fatty acid oxidation of the heart / Clifford D.L. Folmes and Gary D. Lopaschuk -- Regulation of mitochondrial fuel handling by the peroxisome proliferator-activated receptors / Mary C. Sugden and Mark J. Holness -- Molecular structure of the mitochondrial citrate transport protein / Ronald S. Kaplan and June A. Mayor -- Regulation of pyruvate and amino acid metabolism / Thomas C. Vary, Wiley W. Souba, and Cristopher J. Lynch -- Amino acids and the mitochondria / Nicola King -- Mechanotransduction of shear-stress at the mitochondria / Abu-Bakr Al-Mehdi -- Formation of reactive oxygen species in mitochondria / Julio F. Turrens -- Mitochondrial calcium : role in the normal and ischaemic/reperfused myocardium / Elinor J. Griffiths ... [et al.] -- Mitochondrial ion channels / Brian O'Rourke -- The mitochondrial permeability transition pore : from molecular mechanism to reperfusion injury and cadioprotection / Andrew P. Halestrap, Samantha J. Clarke, and Igor Khalilin -- The apoptotic mitochondrial pathway : modulators, interventions, and clinical implications / M.-Saadeh Suleiman and Stephen W. Schaffer -- The role of mitochondria in necrosis following myocardial ischemia-reperfusion / Elizabeth Murphy and Charles Steenbergen -- Mitochondria and their role in ischemia/reperfusion injury / Sebastian Phillip, James M. Downey, and Michael V. Cohen -- Mitochondrial DNA damage and repair / Inna N. Shokolenko, Susan P. Ledoux, and Glenn L. Wilson.
  • 2005 CRCnetBASE
    edited by Carolyn D. Berdanier.
    Introduction to mitochondria / Carolyn D. Berdanier -- Mitochondrial bioenergetics in intact tissue / Richard L. Veech -- The human mitochondrial genome: mechanisms of expression and maintenance / Jan-Willem Taanman and Sion Llewellyn Williams -- Oxidative phosphorylation disease: diagnosis and pathogenesis / John M. Shoffner -- Using mitochondrial DNA in population surveys / Carolyn D. Berdanier -- Mitochondrial DNA mutation, oxidative stress, and alteration of gene expression in human aging / Hsin--Chen Lee and Yau-Huei Wei -- Mitochondrial DNA in cardiomyopathies / Nobuakira Takeda -- Mitochondria in Diabetes mellitus / Hong Kyu Lee -- Drugs, nutrients, and hormones in mitochondrial function / Carolyn D. Berdanier -- Bacterial FF ATP synthase as a model for complex V / Brian D. Cain, Tammy Bohannon Grabar, and Depa Bhatt -- Cybrids in the study of animal mitochondrial genetics and pathology / Ian A. Trounce and Carl A. Pinkert -- Animal modeling: from transgenesis to transmitochondrial models / Carl A. Pinkert and Ian A. Trounce -- BHE/Cdb rats as tools to study mitochondrial gene expression / Carolyn D. Berdanier and Moon-Jeong Chang Kim -- Appendix 1 -- Appendix 2.
  • 2013 Springer
    Lee-Jun C. Wong, editor.
    Mitochondrial cytopathies are mutations in the inherited maternal mitochondrial genome, or the nuclear DNA-mutation. Mitochondrial respiratory chain disorders (RCD) are a group of genetically and clinically heterogeneous diseases, due to the fact that protein components of the respiratory chain are encoded by both mitochondrial and nuclear genomes and are essential in all cells. In addition, the biogenesis, structure and function of mitochondria, including DNA replication, transcription, and translation, all require nuclear encoded genes. Since mitochondria are present in every cell, every tissue, mitochondrial disorders usually affects multiple organs. Mitochondrial Disorders Caused by Nuclear Genes discusses the biochemical, molecular, clinical, and genetic aspects of complex dual genome mitochondrial disorders. Chapters include genes involved in mitochondrial DNA biogenesis and maintenance of mitochondrial DNA integrity, complex subunits and assembly genes, and mitochondrial protein translation related diseases.
  • 2014 ScienceDirect
    volume editor, Anne N. Murphy, David C. Chan.
  • 2009 ScienceDirect
    edited by William S. Allison and Immo E. Scheffler.
    Also available: Print – 2009
  • 2009 ScienceDirect
    edited by William S. Allison, Anne N. Murphy.
    Also available: Print – 2009
  • 2012 Springer
    Bernhard Kadenbach, editor.
    Introduction to Mitochondrial Oxidative Phosphorylation / Bernhard Kadenbach -- Mitochondrial Dynamics: The Intersection of Form and Function / Andrew Ferree and Orian Shirihai -- Biogenesis of Mitochondrial Proteins / Johannes M. Herrmann, Sebastian Longen, Daniel Weckbecker and Matthieu Depuydt -- Assembly Factors of Human Mitochondrial Respiratory Chain Complexes: Physiology and Pathophysiology / Daniele Ghezzi and Massimo Zeviani -- Supramolecular Organisation of the Mitochondrial Respiratory Chain: A New Challenge for the Mechanism and Control of Oxidative Phosphorylation / Giorgio Lenaz and Maria Luisa Genova -- Molecular Mechanisms of Superoxide Production by the Mitochondrial Respiratory Chain / Stefan Dröse and Ulrich Brandt -- Studies on the Function and Regulation of Mitochondrial Uncoupling Proteins / Richard K. Porter -- Evolution of the Couple Cytochrome c and Cytochrome c Oxidase in Primates / Denis Pierron, Derek E. Wildman, Maik Hüttemann, Thierry Letellier and Lawrence I. Grossman -- Reaction Mechanism of Mammalian Mitochondrial Cytochrome c Oxidase / Shinya Yoshikawa, Kazumasa Muramoto and Kyoko Shinzawa-Itoh -- Phosphorylation of Mammalian Cytochrome c and Cytochrome c Oxidase in the Regulation of Cell Destiny: Respiration, Apoptosis, and Human Disease / Maik Hüttemann, Icksoo Lee, Lawrence I. Grossman, Jeffrey W. Doan and Thomas H. Sanderson -- Individual Biochemical Behaviour Versus Biological Robustness: Spotlight on the Regulation of Cytochrome c Oxidase / Rabia Ramzan, Petra Weber, Bernhard Kadenbach and Sebastian Vogt -- Bigenomic Regulation of Cytochrome c Oxidase in Neurons and the Tight Coupling Between Neuronal Activity and Energy Metabolism / Margaret T. T. Wong-Riley -- Cytochrome c Oxidase and Its Role in Neurodegeneration and Neuroprotection / Susanne Arnold -- The Many Clinical Faces of Cytochrome c Oxidase Deficiency / Salvatore DiMauro, Kurenai Tanji and Eric A. Schon.
  • 2013 Springer
    Andrea Strasser, Hans-Joachim Wittmann.
    This practical introduction to molecular modelling of GPCRs covers core principles, and moves on to present advanced GPCR modelling techniques. Includes data on crystal structures and essential amino acid sequences, as well as LINUX commands and examples.
  • 2010
    Samantak Ghosh.
    This thesis discusses a number of projects involving the use of modified nucleotides and oligonucleotides in addressing some basic science questions and some clinical and technological applications.The first chapter details our efforts at using telomere-encoding circular DNA in elongating zebrafish telomeres. We microinjected our synthetic circular DNA into zebrafish embryos and studied their telomere length 24 hrs later. Using Quantitative Fluorescence in situ Hybridization (Q-FISH) as the analytical tool to determine telomere length, we observed no significant difference in telomere length between the group injected with the synthetic DNA and the control group. In the second chapter we studied the potential of a non-polar shape mimic of iodo-uracil as an imager of tumors. We continued discussing our work with non-polar nucleotide isosteres in the third chapter where we used them in investigating a novel active site in polymerase from Pyrococcus furiosus (Pfu). From our studies we concluded that although shape was an important factor in distinguishing bases, this binding site also employed hydrogen bonding to identify nucleoabases. The lack of any recognition of the syn-oxidized bases suggested that the enzyme preferred to recognize bases in the anti conformation rather than syn. In the fourth chapter we were interested in understanding the factors determining the fidelity and selectivity observed in RNA Polymerase II mediated transcription. Once again we used non-polar shape mimics of thymidine (dF) and adenine (dQ) to study the importance of shape and hydrogen bonding. We observed that the thymidine mimic was recognized better by the RNA Polymerase II active site than the mis-match bases. The adenine isostere on the other hand, was poorly recognized. This preliminary study demonstrates the importance of both shape and hydrogen bonding. The last chapter discusses our studies using polyfluorophores on a DNA backbone to detect gases. Following a combinatorial method, a library of oligodeoxyfluorosides (ODFs) were synthesized from which sensors of gases were selected. Using this method we were able to select optical sensors for a diverse set of small molecules in vapor state.
  • 2009 Springer
    Kathleen Sakamoto, Eric Rubin, editors.
  • 2011
    Frank Curtis Brown.
    Mannose 6-phosphate receptors (MPRs) deliver lysosomal acid hydrolases to the endosomal pathway and are then returned from late endosomes to the trans-Golgi network (TGN) for another round of transport. The long coiled-coil protein GCC185 tethers MPR-containing vesicles at the TGN. In the second chapter of this thesis, recent progress in the study of tethers in other laboratories is reviewed. The third chapter describes a mechanism by which GCC185 may tether MPR-containing vesicles. Distinct domains required for the two known functions of this protein, maintenance of Golgi structure and tethering MPR transport vesicles, were identified. The domain needed for vesicle tethering was shown to bind the clathrin adaptor AP-1. Moreover, data are presented showing the presence of AP-1 on transport vesicles en route to the Golgi complex. These data suggest that GCC185, which is localized to the TGN through Arl1- and Rab6-GTPase binding interactions at its C-terminus, engages incoming transport vesicles by binding AP-1 via an internal coiled-coil domain near central, putative "hinge" regions of the protein. Work presented in the fourth chapter describes progress toward understanding the Golgi fragmentation phenotype of cells depleted of GCC185. In addition to the C-terminal localization domain, an N-terminal domain required for Golgi structure maintenance was identified. In addition, GCC185 binding sites for numerous Golgi-localized Rab GTPases were identified, suggesting a model in which GCC185 can make lateral connections across the Golgi ribbon in order to maintain Golgi structure or promote a fused state in the equilibrium between membrane fusion and fission characteristic of the Golgi complex.
  • 2010 Springer
    Jeannine Yon-Kahn, Guy Herve.
  • 2011
    Soren Joseph Peterson.
    From a structural standpoint, one of the most characteristic general design elements of the mammalian organism is its tubular nature. The lung and circulatory system shuttle oxygen and nutrients to target tissues and allow for the excretion of waste products. In some ways, the vasculature lies at the center of human physiology--its passageways provide the infrastructure for maintaining homeostasis. Despite the importance of tubular networks in human health and disease, we have a poor understanding of many aspects of the genetic, molecular, and cellular programs controlling the development of these complex structures. The Drosophila melanogaster tracheal system, an elaborate network of hollow epithelial tubes, transports gases to and from target tissues. The tracheal system, with its simple structure, tractable genetics, and substantial experimental toolkit has emerged as an excellent model system for studying questions with relevance to more complex tubular systems. During development, tracheal branches ramify on the surface of target tissues, providing oxygen to every cell in the body. However, in a phenomenon unique to the Drosophila flight muscle, trachea are also present within plasma membrane invaginations deep below the muscle's outer extremities and have been described to surround every mitochondria of the flight muscle, thus coupling oxygen delivery directly to aerobic respiration at the mitochondrion. Although the presence of trachea within flight muscle membrane invaginations has been described for over 150 years, the developmental progression and cellular and molecular basis of this subcellular targeting process is unknown. In Chapter 2, we show that tracheal branches invade the developing flight muscle Transverse (T)-tubule plasma membrane invagination system during a brief period of pupal development. Branchless (Bnl) FGF, a fibroblast growth factor that functions as a chemoattractant, is required in the flight muscle, and its cognate receptor, Breathless (Btl) FGFR, is required in trachea for the tracheal invasion process to occur. Whereas Bnl FGF is localized to all flight muscle plasma membranes prior to tracheal invasion, during invasion Bnl FGF localizes preferentially to the T-tubule and is excluded from the surrounding plasma membrane. In addition to Bnl FGF, core polarity regulators commonly found on basolateral membranes in epithelial cells also preferentially localize to the T-tubule network during tracheal invasion. We find that depletion of AP-1[gamma], targeting machinery required for basolateral secretion in Drosophila epithelia, can also reroute Bnl FGF secretion to the outer plasma membrane and away from T-tubule openings, shifting trachea to the plasma membrane and away from T-tubules. We propose that (1) polarized secretion of Bnl FGF to the T-tubule guides tracheal branches into the T-tubule network and that (2) polarized Bnl FGF secretion is established via the redeployment of ancestral basolateral secretion pathways to the T-tubules, a membrane domain having molecular signatures of epithelial basolateral domains. To our knowledge, compartmentalized secretion of Bnl FGF to flight muscle T-tubule membranes is the first example of polarized subcellular secretion of a growth factor with functional consequences for the development of another tissue. In Chapter 3, we examine the molecular machinery involved in maintaining the polarized secretion of Bnl FGF during tracheal invasion. We find a host of secretory machinery, including several Rabs, Myosin V, an actin nucleator, and others, to be involved in the secretion of Bnl-FGF to the T-tubule during tracheal invasion. From these data we propose a molecular model to explain polarized Bnl FGF secretion at the T-tubule. Why is the flight muscle the only tissue invaded by trachea? In Chapter 4, we find that the flight muscle is structurally adapted to allow its T-tubule plasma membrane invagination network to be co-opted by migrating tracheal processes. In contrast to other muscles, the flight muscle T-tubule network forms large holes on the muscle surface as part of its development. The tracheal invasion process in the flight muscle is therefore the consequence not only of a polarized secretion process as discussed in Chapter 2, but also a structurally distinct stage of flight muscle development. In the final chapter, we search for the fine tracheal tubes reported to target and form contacts on the flight muscle mitochondria. We find an extracellular protein-based lattice of the appropriate diameter and localization to potentially represent the reported tracheal extensions to the mitochondria and propose several models of protein lattice formation. We hope that insight derived from this work spurs future investigators in a host of areas. The exquisite example of targeting to specific membrane domains dependent on a subcellular chemoattractant gradient demonstrated by the invading tracheal branch may provide insight into a number of developmental scenarios where fine targeting of different cells is required. We also hope that our finding regarding polarized secretion in muscle inspires new ways to think about the assembly of muscle membranes during development and disease states.
  • 2003 CRCnetBASE
    Tom Sanders and Peter Emery.
  • v. 1-2, 1985-86.
    George Feuer, Felix A. De la Iglesia.
    Status: Not Checked OutLane Catalog Record
  • 2013 Springer
    Ahmad M. Khalil, Jeff Coller, editors.
    Chromatin regulation by long non-coding RNAs / Daniel C. Factor, Paul J. Tesar and Ahmad M. Khalil -- Regulatikon of eukaryotic cell differentiation by long non-coding RNAs / Juan R. Alvarez-Dominguez, Wenqian Hu and Harvey F. Lodish -- Roles of long non-coding RNAs in X-chromsome inactivation / J. Mauro Calabrese and Terry Magnuson -- Roles of long non-coding RNAs in genomic imprinting / Kristen Martins-Taylor and Stormy J. Chamberlain -- Dysregulation of long non-coding RNAs in human disease / Nianwei Lin and Tariq M. Rana -- Functions of long non-coding RNAs in non-mamallian systems / Alex Tuck and David Tollervey -- Emerging technologies to study long non-coding RNAs / Fereshteh Jahaniani ... [et al.]
  • 2006 Springer
    Markus J. Tamás, Enrico Martinoia (eds.).
  • 2006 Springer
    editor, Matthias Gaestel ; contributors, S. Alberti ... [et al.].
  • 2014 Springer
    Barbara Burtness, Erica Golemis, editors.
    Head and neck cancers, involving sites from the nasopharynx to the subglottic larynx, are frequently devastating cancers that afflict patients around the world. These cancers are frequently locally advanced prior to detection, and require multimodality therapy that is associated with high morbidity. As this book addresses this difficult disease, it accomplishes three main goals. First, it introduces the etiology and subclasses of squamous cell carcinomas of the head and neck (SCCHNs), and how these factors affect prognosis. Although habitual exposures to tobacco, alcohol, and other agents have historically been the main causes of SCCHN, a rising proportion of oropharynx cancers arise from transforming human papillomavirus (HPV) infection. These two broad classes of SCCHN have significant differences in disease profile and response to treatment, as we discuss. Second, it summarizes the current state of understanding of the genetic, epigenetic and protein expression changes associated with the various classes of SCCHN. In the past decade, disease pathogenesis of SCCHN has been appreciated to involve deregulation of multiple tumor pathways, including the receptor tyrosine kinases (RTKs) EGFR, ERBB2, ERBB3, c-MET, and IGF1R; transforming growth factor β (TGFβ); Notch; cytoplasmic signaling proteins including PTEN, PI3K, JAK/STAT, and Wnt-responsive β-catenin; mutation control systems, including p53 and the DNA damage repair (DDR) machinery; and hypoxic response. The specific understanding of the action of these proteins in SCCHN is presented here. Finally, this book defines potential therapeutic targets for improved management of the disease in the future, discussing prospects for improved prediction of prognosis.
  • 2008 Springer Protocols
    edited by Paul T. Sharpe and Ivor Mason.
    The mouse as a developmental model / Paul T. Sharpe -- Culture of postimplantation mouse embryos / Paul Martin and David L. Cockroft -- Organ culture in the analysis of tissue interactions / Irma Thesleff and Carin Sahlberg -- Treatment of mice with retinoids in vivo and in vitro / Gillian M. Morriss-Kay -- Analysis of skeletal ontogenesis through differential staining of bone and cartilage / Michael J. Depew -- Cell grafting and labeling in postimplantation mouse embryos / Gabriel A. Quinlan ... [et al.] -- Production of transgenic rodents by the microinjection of cloned DNA into fertilized one-celled eggs / David Murphy -- Cre recombinase mediated alterations of the mouse genome using embryonic stem cells / Anna-Katerina Hadjantonakis, Melinda Pirity, and András Nagy -- Gene trapping in mouse embryonic stem cells / Jane Brennan and William C. Skarnes -- Application of lacZ transgenic mice to cell lineage studies / Catherine M. Watson ... [et al.] -- Transgenic RNA interference to investigate gene function in the mouse / Tilo Kunath -- Mouse primordial germ cells : isolation and in vitro culture / Patricia A. Labosky and Brigid L.M. Hogan -- Gene transfer to the rodent embryo by retroviral vectors / Marla B. Luskin -- The avian embryo : an overview / Ivor Mason -- Chick embryos : incubation and isolation / Ivor Mason -- New culture / Amata Hornbruch -- EC culture : a method to culture early chick embryos / Andrea Streit -- Grafting Henson's node / Claudio D. Stern -- Microsurgical manipulation of the notochord / Lúcia E. Alvares ... [et al.] -- Transplantation of avian neural tissue / Sarah Guthrie -- Grafting of apical ridge and polarizing region / Cheryl Tickle -- Tissue recombinations in collagen gels / Marysia Placzek -- Quail-chick chimeras / Marie-Aimée Teillet, Catherine Ziller, and Nicole M. Le Douarin -- Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo / Jonathan D.W. Clarke -- Gene transfer in avian embryos using replication-competent retroviruses / Cairine Logan and Philippa Francis-West -- Electroporation in avian embryos / Jun-ichi Funahashi and Harukazu Nakamura -- An overview of Xenopus development / Michael Jones and James C. Smith -- Mesoderm induction assays / C. Michael Jones and James C. Smith -- Experimental embryological methods for analysis of neural induction in the amphibian / Ray Keller, Ann Poznanski, and Tamira Elul -- A method for generating transgenic frog embryos / Shoko Ishibashi, Kristin L. Kroll, and Enrique Amaya -- Axolotl/newt / Malcolm Maden -- The zebrafish : an overview of its early development / Nigel Holder and Qiling Xu -- Small-scale marker-based screening for mutations in zebrafish development / Peter D. Currie, Thomas F. Schilling, and Philip W. ingham -- Microinjection and cell transplantation in zebrafish embryos / Qiling Xu, Derek Stemple, and Katherine Joubin -- Recent advances in meganuclease- and transposon-mediated transgenesis of medaka and zebrafish / Clemens Grabher and Joachim Wittbrodt -- Retinoids in nonmammalian embryos / Malcolm Maden -- Protochordates / Peter W.H. Holland and Hiroshi Wada -- Subtractive hybridization and construction of cDNA libraries / Bruce Blumberg and Juan Carlos Izpisúa Belmonte -- Differential display of eukaryotic mRNA / Antonio Tugores and Juan Carlos Izpisúa Belmonte -- Using DNA microarrays / Clare Pritchard, Peter Underhill, and Andy Greenfield -- Profiling gene transcription in the developing embryo : microarray analysis on gene chips / David Chambers and Andrew Lumsden -- RT-PCR on embryos using degenerate oligonucleotide primers / Anthony Graham -- Single-cell RT-PCR cDNA subtraction / Ebrahim Sakhinia ... [et al.] -- In-situ hybridization of rdioactive riboprobes to RNA in tissue sections / Radma Mahmood and Ivor Mason -- In-situ hybridization and immunohistochemistry in whole embryos / Carol Irving -- Wholemount in situ hybridization to Xenopus embryos / C. Michael Jones and James C. Smith -- Wholemount in situ hybridization to Amphioxus embryos / Peter W.H. Holland -- In-situ hybridization to sections (nonradioactive) / Maria Rex and Paul J. Scotting -- Immunohistochemistry using polyester wax / Andrew Kent -- Immunohistochemistry on whole embryos / Ivor Mason -- Whole embryo assays for programmed cell death / Anthony Graham -- Protein techniques : immunoprecipitation, in vitro kinase assays, and western blotting / Clive Dickson -- Microscopy and photmicrography techniques / Richard J.T. Wingate.
  • 2008 Springer
    edited by Barbara A. Burleigh, Dominique Soldati-Favre.
    Current and emerging approaches to studying invasion in apicomplexan parasites / Jeffrey Mital and Gary E. Ward -- Microneme proteins in apicomplexans / Vern B. Carruthers and Fiona M. Tomley -- Erythrocyte invasion by plasmodium falciparum: multiple ligand-receptor interactions and phenotypic switching / Manoj T. Duraisingh ... [et al.] -- Role of the gp85/trans-sialidase superfamily of glycoproteins in the interaction of trypanosoma cruzi with host structures / Maria Jølia M. Alves and Walter Colli -- Calcium regulation and signaling in apicomplexan parasites / Kisaburo Nagamune ... [et al.] -- Trypanosoma cruzi: parasite and host cell signaling during the invasion process / Nobuko Yoshida and Mauro Cortez -- Host cell actin remodeling in response to cryptosporidium / Steven P. O'Hara ... [et al.] -- Host cell actin remodeling in response to trypanosoma cruzi: trypomastigote versus amastigote entry / Renato A. Mortara ... [et al.] -- Actin/myosin-based gliding motility in apicomplexan parasites / Kai Matuschewski and Herwig Schøler -- Roles of proteases during invasion and egress by plasmodium and toxoplasma / Timothy J. Dowse ... [et al.] -- Roles of naturally occurring protease inhibitors in the modulation of host cell signaling and cellular invasion by trypanosoma cruzi / Julio Scharfstein and Ana Paula C.A. Lima -- Biogenesis of and activities at the toxoplasma gondii parasitophorous vacuole membrane / Anthony P. Sinai. The role of host cell lysosomes in trypanosoma cruzi invasion / G. Adam Mott and Barbara A. Burleigh -- Leishmania invasion and phagosome biogenesis / Robert Lodge and Albert Descoteaux -- Plasmodium sporozoite passage across the sinusoidal cell layer / Ute Frevert ... [et al.] -- Transepithelial migration by toxoplasma / Antonio Barragan and Niclas Hitziger -- The microsporidian polar tube and its role in invasion / Frederic Delbac and Valerie Polonais -- Intestinal invasion by entamoeba histolytica / Shahram Solaymani-Mohammadi and William A. Petri, Jr.
  • 2015 Springer Protocols
    edited by Andreas Kukol, University of Hertfordshire, Hatfield, Hertfordshire, UK.
    Molecular dynamics simulations / Erik R. Lindahl -- Monte Carlo simulations / David J. Earl and Michael W. Deem -- Hybrid quantum and classical methods for computing kinetic isotope effects of chemical reactions in solutions and in enzymes / Jiali Gao ... [et al.] -- Comparison of protein force fields for molecular dynamics simulations / Olgun Guvench and Alexander D. MacKerell, Jr. -- Normal modes and essential dynamics / Steven Hayward and Bert L. de Groot -- Calculation of absolute protein-ligand binding constants with the molecular dynamics free energy perturbation method / Hyung-June Woo -- Free energy calculations applied to membrane proteins / Christophe Chipot -- Molecular dynamics simulations of membrane proteins / Philip C. Biggin and Peter J. Bond -- Membrane-associated proteins and peptides / Marc F. Lensink -- Implicit membrane models for membrane protein simulation / Michael Feig -- Comparative modeling of proteins / Gerald H. Lushington -- Transmembrane protein models based on high-throughput molecular dynamics simulations with experimental constratints / Andrew J. Beevers and Andreas Kukol -- Nuclear magnetic resonance-based modeling and refinement of protein three-dimensional structures and their complexes / Gloria Fuentes, Aalt D.J. van Dijk, and Alexandre M.J.J. Bonvin -- Conformational changes in protein function / Haiguang Liu ... [et al.] -- Protein folding and unfolding by all-atom molecular dynamics simulations / Hongxin Lei and Yong Duan -- Modeling of protein misfolding in disease / Edyta B. Małolepsza -- Identifying putative drug targets and potential drug leads : starting points for virtual screening and docking / David S. Wishart -- Receptor flexibility for large-scale in silico ligand screens : chances and challenges / B. Fischer, H. Merlitz, and W. Wenzel -- Molecular docking / Garrett M. Morris and Marguerita Lim-Wilby.
  • 2008 Springer Protocols
    Andreas Kukol, [editor].
    Molecular dynamics simulations / Erik R. Lindahl -- Monte Carlo simulations / David J. Earl and Michael W. Deem -- Hybrid quantum and classical methods for computing kinetic isotope effects of chemical reactions in solutions and in enzymes / Jiali Gao ... [et al.] -- Comparison of protein force fields for molecular dynamics simulations / Olgun Guvench and Alexander D. MacKerell, Jr. -- Normal modes and essential dynamics / Steven Hayward and Bert L. de Groot -- Calculation of absolute protein-ligand binding constants with the molecular dynamics free energy perturbation method / Hyung-June Woo -- Free energy calculations applied to membrane proteins / Christophe Chipot -- Molecular dynamics simulations of membrane proteins / Philip C. Biggin and Peter J. Bond -- Membrane-associated proteins and peptides / Marc F. Lensink -- Implicit membrane models for membrane protein simulation / Michael Feig -- Comparative modeling of proteins / Gerald H. Lushington -- Transmembrane protein models based on high-throughput molecular dynamics simulations with experimental constraints / Andrew J. Beevers and Andreas Kukol -- Nuclear magnetic resonance-based modeling and refinement of protein three-dimensional structures and their complexes / Gloria Fuentes, Aalt D.J. van Dijk, and Alexandre M.J.J. Bonvin -- Conformational changes in protein function / Haiguang Liu ... [et al.] -- Protein folding and unfolding by all-atom molecular dynamics simulations / Hongxin Lei and Yong Duan -- Modeling of protein misfolding in disease / Edyta B. Małolepsza -- Identifying putative drug targets and potential drug leads : starting points for virtual screening and docking / David S. Wishart -- Receptor flexibility for large-scale in silico ligand screens : chances and challenges / B. Fischer, H. Merlitz, and W. Wenzel -- Molecular docking / Garrett M. Morris and Marguerita Lim-Wilby.
  • 2011
    Emily Lawson Egeler.
    Cellular maintenance of protein homeostasis , or proper protein folding, is essential for normal cellular function. Accumulation of misfolded protein can lead to disease and even cell death. The ubiquitin-proteasome system plays a central role in processing cellular proteins for degradation, but little is currently known about how misfolded cytosolic proteins are recognized by protein quality control machinery and targeted for degradation in mammalian cells. In the work presented here, we work with a model protein substrate that is unstable when expressed in cells, but stabilized by addition of a small cell-permeable ligand. Using purified protein, we show that unstable mutants are poorly folded or rapidly sampling unfolded states. The propensity to unfold in vitro correlates with observed instability when expressed in cells. We also show that these substrates are rapidly ubiquitinated en route to degradation at the proteasome in a ligand-dependent manner. We conducted a mass spectrometry screen to identify endogenous protein interactors with the model substrate, which identified a few annotated ubiquitin-proteasome system components. The findings presented herein correlate observed cellular instability to both the propensity of the protein to unfold and the extent of ubiquitination in the ligand-free state. We propose that this protein is a ligand-dependent substrate for the protein quality control machinery, providing an excellent substrate into further query the mechanisms of protein quality control.
  • 2015 Springer
    Eugene G. Hrycay, Stelvio M. Bandiera, editors.
    Foreword -- 1. Monooxygenase, Peroxidase and Peroxygenase Properties and Reaction Mechanisms of Cytochrome P450 Enzymes -- 2. Oxidizing Intermediates in P450 Catalysis: A Case for Multiple Oxidants -- 3. Current Approaches for Investigating and Predicting Cytochrome P450 3A4-Ligand Interactions -- 4. Acyl-Carbon Bond Cleaving Cytochrome P450 Enzymes: CYP17A1, CYP19A1 and CYP51A1 -- 5. Regioselective Versatility of Monooxygenase Reactions Catalyzed by CYP2B6 and CYP3A4: Examples With Single Substrates -- 6. Cytochrome P450 Enzymes in the Bioactivation of Polyunsaturated Fatty Acids and their Role in Cardiovascular Disease -- 7. Monooxygenation of Small Hydrocarbons Catalyzed by Bacterial Cytochrome P450s -- 8. Use of Chemical Auxiliaries to Control P450 Enzymes for Predictable Oxidations at Unactivated C-H Bonds of Substrates,- 9. Cytochrome P450 Enzymes and Electrochemistry: Crosstalk with Electrodes as Redox Partners and Electron Sources -- 10. Mechanistic Basis of Electron Transfer to Cytochromes P450 by Natural Redox Partners and Artificial Donor Constructs -- 11. Biological Diversity of Cytochrome P450 Redox Partner Proteins -- 12. Cytochrome P450cin (CYP176A1) -- 13. Fungal Unspecific Peroxygenases: Heme-Thiolate Proteins that Combine Peroxidase and Cytochrome P450 Properties.
    Also available: Print – 2015
  • 2012 Springer
    Sunil C. Kaul, Renu Wadhwa, editors.
    Part 1. Structure and Function of Mortalin / Birth of Mortalin: Multiple Names, Niches and Functions Connecting Stress, Senescence and Cancer / Renu Wadhwa and Sunil C. Kaul -- Mortalin's Machinery / Custer C. Deocaris, Sunil C. Kaul and Renu Wadhwa -- The Role of Mortalin in Iron Homeostasis / Wen-I Luo and James A. Cowan -- Functional Characteristics of Mortalin / Walter A. Baseler, Tara L. Croston and John M. Hollander -- Part 2. Mortalin in Evolution2 Deocaris / Mortalin and Drosophila DmHsp22: Two Mitochondrial Chaperones Regulating Aging and Carcinogenesis / Marie Le Pécheur, Geneviève Morrow and Robert M. Tanguay -- Mortalin in Invertebrates and The Induction of Apoptosis by Wild-Type p53 Following Defeat of Mortalin-Based Cytoplasmic Sequestration in Cancerous Clam Hemocytes / Charles W. Walker, Ben Low and S. Anne Böttger -- Mortalin and Stem Cells: A Study from Planarians / Renata Batistoni -- Part 3. Mortalin in Health and Disease / Mortalin in Cell Protection from Immune Attack / Moran Saar, Oren Moskovich and Zvi Fishelson -- Mortalin in Neurological Diseases / Jinghua Jin, Jing Zhang, Travis J. Cook and Jake G. Hoekstra -- Loss of Mortalin Function in Parkinson's Disease-Supporting the Mitochondrial Pathway of Neurodegeneration / Lena F. Burbulla and Rejko Krüger -- Hsp75/mortalin and Protection from Ischemic Brain Injury / Robin E. White, Yi-Bing Ouyang and Rona G. Giffard -- Catecholamine Regulated Protein (CRP40), A Splice Variant of Mortalin-2: Functional Role in CNS Disorders / Joseph P. Gabriele, Sarah E. Groleau, Ritesh P. Daya, Zdenek B. Pristupa and Ram K. Mishra -- Chaperonopathies: Diseases in Which Mortalin and Other Hsp-Chaperones Play a Role in Etiology and Pathogenesis / Alberto J. L. Macario, Francesco Cappello and Everly Conway de Macario -- Part 4. Mortalin and Cancer / Many Faces of Mortalin and Tid1 / Ohad Iosefson and Abdussalam Azem -- Mortalin: A Positive Regulator of Centrosome Duplication and Amplification / Masayuki Kanai and Kenji Fukasawa -- Mortalin Expression in Normal and Neoplastic Tissues / Angheliki Nomikos, Sinclair R. Dundas and Graeme I. Murray -- Mortalin-p53 Interaction as a Target for Liver Cancer Therapy / Wen-Jing Lu, Nikki P. Lee, Renu Wadhwa and John M. Luk -- Mortalin Targeting Gadgets for Cancer Therapy / Chae-Ok Yun and Renu Wadhwa -- Part 5. Mortalin Based Technologies / Cell Internalizing Anti-Mortalin Antibody for Generation of Illuminating MSCs for Long-Term In vitro and In vivo Tracking / Toshimasa Uemura, Masanori Nishi, Sunil C. Kaul and Renu Wadhwa -- Mortalin Staining Pattern as a Reporter for Cell Based Anti-Cancer Drug Screening / Ran Gao, Zeenia Kaul, Tomoko Yaguchi and Renu Wadhwa -- Cell Internalizing Anti-mortalin Antibody as a Nanocarrier / Zeenia Kaul, Tomoko Yaguchi, Renu Wadhwa and Sunil C. Kaul.
  • 2011
    Dina Finan.
    Molecular motors are proteins that use energy from ATP hydrolysis to walk along cytoskeletal tracks, often transporting cargo as they move. A substantial amount of work has been done to study their catalytic domains, obtaining in vitro biochemical, structural, and biophysical measurements of motors' stepping behavior. Compared to this wealth of data, much less is known about how they actually work in cells. Regions of the motor proteins called cargo-binding domains are responsible for mediating their interactions with other molecules, but we know relatively little about what specific cargoes they associate with, the biochemical basis of these interactions, how they are regulated, and thus the precise functions of the motors in vivo. We have focused on two unconventional myosin motors in Drosophila, V and VI, both of which play important roles in fly development, and whose cargo-binding domains have been relatively unexplored. We use a proteomics approach to identify multiple novel binding partners for both myosins. The identities and biochemical characterization of a small fraction of the candidate binding proteins have begun to shed light on pathways in which the motors are likely to be involved. We have focused on roles for myosin VI in protein secretion and intracellular trafficking, and it seems likely that myosin V will play distinct roles in trafficking of ribonucleoprotein complexes. The discovery of binding partners is an important step towards understanding what motors do in cells, and we expect that this method will be useful for other motors in the future. We also hope that these findings will continue to elucidate biological roles of myosin V and VI.
  • 2011 Future Med
    editor, Leonidas C. Platanias.
    mTOR inhibitors in cancer treatment / Leonidas C. Platanias -- Overview and mechanism of action of mTOR inhibitors / Stéphane Oudard -- mTOR inhibitors in renal cell carcinoma / Piotr J. Wysocki -- mTOR inhibitors in hepatocellular carcinoma / Gerhard Treiber & Thomas Wex -- mTOR inhibitors in ovarian cancer / Susana M. Campos -- mTOR inhibitors in breast cancer / Ingrid A. Mayer -- mTOR inhibitors in Hodgkin's and non-Hodgkin's lymphomas / Sonali M. Smith.
  • 2012 Springer Protocols
    edited by Michael A. McGuckin, David J. Thornton.
    Mucin methods : genes encoding mucins and their genetic variation with a focus on gel-forming mucins / Karine Rousseau and Dallas M. Swallow -- Gel-forming and cell-associated mucins : preparation for structural and functional studies / Julia R. Davies, Claes Wickstrom, and David J. Thornton -- Detecting, visualising, and quantifying mucins / Ceri A. Harrop, David J. Thornton, and Michael A. McGuckin -- Mass spectrometric analysis of mucin core proteins / Mehmet Kesimer and John K. Sheehan -- O-glycoprotein biosynthesis : site localization by edman degradation and site prediction based on random peptide substrates / Thomas A. Gerken -- Analysis of assembly of secreted mucins / Malin E.V. Johansson and Gunnar C. Hansson -- MUC1 membrane trafficking : protocols for assessing biosynthetic delivery, endocytosis, recycling, and release through exosomes / Franz-Georg Hanisch [and others] -- Glycomic work-flow for analysis of mucin o-linked Oligosaccharides / Catherine A. Hayes [and others] -- O-glycomics : profiling and structural analysis of mucin-type o-linked glycans / Isabelle Breloy -- O-glycoproteomics : site-specific o-glycoprotein analysis by CID/ETD electrospray ionization tandem mass spectrometry and top-down glycoprotein sequencing by in-source decay MALDI mass spectrometry / Franz-Georg Hanisch -- Analysing mucin degradation / Stephen D. Carrington [and others] -- Assessment of mucus thickness and production in situ / Lena Holm and Mia Phillipson -- Preservation of mucus in histological sections, immunostaining of mucins in fixed tissue, and localization of bacteria with FISH / Malin E.V. Johansson and Gunnar C. Hansson -- Ex vivo measurements of mucus secretion by colon explants / Jenny K. Gustafsson, Henrik Sjovall, and Gunnar C. Hansson -- Establishment of respiratory air-liquid interface cultures and their use in studying mucin production, secretion and function / David B. Hill and Brian Button -- Studying mucin secretion from human bronchial epithelial cell primary cultures / Lubna H. Abdullah [and others] -- Assessment of intracellular mucin content in vivo / Lucia Piccotti, Burton F. Dickey, and Christopher M. Evans -- Techniques for assessment of interactions of mucins with microbes and parasites in vitro and in vivo / Yong H. Sheng [and others] -- Assessing mucin expression and function in human ocular surface epithelia in vivo and in vitro / Pablo Argueso and Ilene K. Gipson.
  • 2013 Future Med
    editors, Joyce Taylor-Papadimitriou, Joy M. Burchell.
    Mucins and cancer / Joyce Taylor-Papadimitriou & Joy M. Burchell -- Introduction to mucins in cancer / Michael A. McGuckin -- Changes in mucin-type O-linked glycosylation in cancer / Joy M. Burchell & Joyce Taylor-Papadimitriou -- Mucins as biomarkers in cancer / Kowa Chen, Ola Blixt & Hans H. Wandall -- Signaling from membrane mucins / Tze Wei Poh & Sandra J. Gendler -- MUC4 and membrane receptors in cancer / Kermit L. Carraway & Coralie A. Carothers Carraway -- MUC16, alias CA125 and ovarian cancer / Evangelia-Ourania Fourkala, Aleksandra Gentry-Maharaj & Usha Menon -- Mucins in metastasis / Sylvain Julien -- How mucins shape antigen-presenting cells to modify immune responses / Sandra J. van Vliet & Yvette van Kooyk -- MUC1 as a therapeutic target in cancer / Jean-Marc Limacher & Bruce Acres -- Index.
  • 2014 Springer Protocols
    edited by David J. Russell.
    Dynamic programming / O. Ufuk Nalbantoglu -- Heuristic alignment methods / Osamu Gotoh -- Objective functions / Haluk Dogan and Hasan H. Otu -- Who watches the watchmen? An appraisal of benchmarks for multiple sequence alignment / Stefano Iantorno ... [et al.] -- BLAST and FASTA similarity searching for multiple sequence alignment / William R. Pearson -- Clustal omega, accurate alignment of very large numbers of sequences / Fabian Sievers and Desmond G. Higgins -- T-coffee : tree-based consistency objective function for alignment evaluation / Cedrik Magi ... [et al.] -- MAFFT : iterative refinement and additional methods / Kazutaka Katoh and Daron M. Standley -- Multiple sequence alignment using probcons and probalign / Usman Roshan -- Phylogeny-aware alignment with PRANK / Ari Loytynoja -- GramAlign : fast alignment driven by grammar-based phylogeny / David J. Russell -- Multiple sequence alignment with DIALIGN / Burkhard Morgenstern -- PicXAA : a probabilistic scheme for finding the maximum expected accuracy alignment of multiple biological sequences / Sayed Mohammad Ebrahim Sahraeian and Byung-Jun Yoon -- Multiple protein sequence alignment with MSAProbs / Yongchao Liu and Bertil Schmidt -- Large-scale multiple sequence alignment and tree estimation using SATe / Kevin Liu and Tandy Warnow -- PRALINE : a versatile multiple sequence alignment toolkit / Punto Bawono and Jaap Heringa -- PROMALS3D : multiple protein sequence alignment enhanced with evolutionary and three-dimensional structural information / Jimin Pei and Nick V. Grishin -- MSACompro : improving multiple protein sequence alignment by predicted structural features / Xin Deng and Jianlin Cheng.
  • 2014 Springer
    Swati Palit Deb, Sumitra Deb, editor.
    This book provides the readers with an overview of research on p53, which has been shown to play a role in numerous crucial biological pathways in normal and cancer cells. Leading scientist in the field, who have all made direct contributions to the understanding of the molecular events underpinning p53 function, have been invited to contribute the various chapters, which discuss the current knowledge of the signaling cascades that are activated by mutations in p53 and overexpression of MDM2, frequently found in human cancer, and are major causes of oncogenesis. This book features chapters on the molecular basis of oncogenesis induced by gain of function mutation of p53, signaling pathways induced by MDM2 overexpression, control of mutant or wild-type p53 function by MDM2 and MDMX, p53 mutation in hereditary cancer, and structural aspects that activate mutant p53 which can be targeted by drug therapy. This book should be useful for scientists at all levels.
  • 2008 Springer
    edited by Lynne M. Coluccio.
    The structural and functional diversity of the myosin family of actin-based molecular motors / M.S. Mooseker and B.J. Foth -- Myosin structure / K.C. Holmes -- The myosin family: Biochemical and kinetic properties / M. El-Mezgueldi and C.R. Bagshaw -- Myosin I / L.M. Coluccio -- Myosin II : Sarcomeric myosins, the motors of contraction in cardiac and skeletal muscles / C. Reggiani and R. Bottinelli -- Smooth-muscle myosin II / C.R. Cremo and D.J. Hartshorne -- Non-muscle myosin II / M. Conti, S. Kawamoto and R.S. Adelstein -- Class III myosins / A. Dosé, J. Lin-Jones and B. Burnside -- Myosin V / J.R. Sellers and L.S. Weisman -- Myosin VI : A multifunctional motor protein / F. Buss and J. Kendrick-Jones -- Myosin VII / A. El-Amraoui, A. Bahloul and C. Petit -- Plant myosins VIII, XI, and XIII / K. Yamamoto -- Class IX myosins / M. Bähler -- Myosin X / M.M. Divito and R.E. Cheney -- Myosin class XIV and other myosins in protists / K. Frénal, B.J. Foth and D. Soldati -- Myosin XVa / E.T. Boger ... [et al.].
  • 2011 Springer Protocols
    edited by Steven A. Toms, Robert J. Weil.
    MALDI-TOF serum profiling using semiautomated serum peptide capture with magnetic reversed phase (C18) beads / Jaco C. Knol and Connie R. Jimenez -- Proteomics of epithelial lining fluid obtained by bronchoscopic microprobe sampling / Lorenza Franciosi [and others] -- Protein identification using nano-HPLC-MS : ESI-MS and MALDI-MS interfaces / Rui Vitorino [and others] -- Three-dimensional peptide fractionation for highly sensitive nanoscale LC-based shotgun proteomic analysis of complex protein mixtures / Sricharan Bandhakavi [and others] -- Nanospray ion mobility mass spectrometry of selected high mass species / Iain Campuzano and Kevin Giles -- Nanoelectrospray-MSn of native and permethylated glycans / Christina Bleckmann, Hildegard Geyer, and Rudolf Geyer -- N-linked global glycan profiling by nanoLC mass spectrometry / Michael S. Bereman and David C. Muddiman -- Applications of nanoscale liquid chromatography coupled to tandem mass spectrometry in quantitative studies of protein expression protein-protein interaction, and protein phosphorylation / Metodi V. Metodiev -- Nano LC-MS/MS : a robust setup for proteomic analysis / Marco Gaspari and Giovanni Cuda -- Nanofluidic devices for rapid continuous-flow bioseparation / Pan Mao and Jianping Fu -- Quantifying attomole amounts of proteins from complex samples by nano-LC and selected reaction monitoring / Thomas Frohlich and Georg J. Arnold -- Sample preparation method for gold nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry / Yen-Hsiu Lin and Wei-Lung Tseng -- Nanostructured TiO2 thin films for phosphoproteomics studies with MALDI mass spectrometry / Federico Torta [and others] -- Nanofilament silicon for matrix-free laser desorption/ionization mass spectrometry / Chia-Wen Tsao and Don L. DeVoe -- Protein nanoarrays for high-resolution patterning of bacteria on gold surfaces / Cait Costello [and others] -- Engineered multifunctional nanotools for biological applications / Mohammed Ibrahim Shukoor [and others] -- Selective capture of phosphopeptides by zirconium phosphonate-magnetic nanoparticles / Liang Zhao, Renan Wu, and Hanfa Zou -- Nanowire biosensors for label-free, real-time, ultrasensitive protein detection / Gengfeng Zheng and Charles M. Lieber -- Generation of anti-infectome/anti-proteome nanobodies / Gholamreza Hassanzadeh-Ghassabeh, Dirk Saerens, and Serge Muyldermans -- Isolation, propagation, and analysis of biological nanoparticles / Michael P. Linnes [and others] -- Functionalized soluble nanopolymers for phosphoproteome analysis / Anton Iliuk [and others] -- Elucidating structural dynamics of integral membrane proteins on native cell surface by hydroxyl radical footprinting and nano LC-MS/MS / Yi Zhu, Tiannan Guo, and Siu Kwan Sze -- Application of electrostatic repulsion hydrophilic interaction chromatography to the characterization of proteome, glycoproteome, and phosphoproteome using nano LC-MS/MS / Piliang Hao, Huoming Zhang, and Siu Kwan Sze.
  • 2006 Springer
    Aldo Clerico, Michele Emdin (eds.) ; foreword by Luigi Donato.
  • 2015 Springer
    Neville Vassallo, editor.
    This edited volume concerns a group of devastating neurological disorders that share a common pathological mechanism, namely the aggregation and deposition of insoluble, proteinaceous lesions, termed 'amyloid'. Examples of cerebral amyloid disorders include common neurodegenerative diseases like Alzheimer's disease-related dementia and Parkinson's disease, as well as other less prevalent conditions like Huntington's disease, cerebral amyloid angiopathy and the transmissible prion disorders. A disease-modifying therapeutic agent is still lacking for all these diseases, and there are no approved therapies that target amyloid formation directly. Nevertheless, a large and complex group of natural aromatic compounds known as polyphenols are rapidly emerging as potentially potent anti-amyloidogenic agents. This book collectively presents a considerable body of experimental and epidemiological evidence from peer-reviewed scientific publications that support a role for natural compounds and herbal extracts in the chemoprevention and therapy of amyloidogenic disorders. Each contribution is written by scientific experts in the relevant field; chapters are devoted to Mediterranean diet and olive oil phenols, traditional Chinese medicine, herbal extracts, polyphenols (with a particular emphasis on epigallocatechin-3-gallate) and bi-flavonoids, amongst others. The topic of this book is relevant to a wide audience, from academia and university students in the biological and chemical sciences, to physicians and allied health professionals, as well as people working in the nutraceutical industry.
    Also available: Print – 2015
  • pt. A-C, 2012. ScienceDirect
    pt. B ScienceDirect
    pt. C ScienceDirect
    edited by David A. Hopwood.
  • 2012 Springer Protocols
    edited by Satyajit D. Sarker, Lutfun Nahar.
    An introduction to natural products isolation -- Initial and bulk extraction of natural products isolation -- Supercritical fluid extraction in natural products analyses -- Accelerated solvent extraction for natural products isolation -- Microwave-assisted extraction in natural products isolation -- An introduction to planar chromatography and its application to natural products isolation -- Isolation of natural products by low-pressure column chromatography -- Isolation of natural products by ion-exchange methods -- Separation of natural products by countercurrent chromatography -- Isolation of natural products by preparative high performance liquid chromatography (prep-HPLC) -- Isolation of natural products by preparative gas chromatography -- Hyphenated techniques and their applications in natural products analysis -- Extraction of plant secondary metabolites -- Isolation of marine natural products -- Isolation of microbial natural products -- Extraction and isolation of saponins -- Extraction and isolation of phenolic compounds -- Scaling-up of natural products isolation -- Follow-up of natural products isolation -- Natural products isolation in modern drug discovery programs.
  • 2012 Springer
    edited by Hans U. Lutz.
    Naturally occurring IgM antibodies to oxidation-specific epitopes / Christoph J. Binder -- Naturally occurring autoantibodies to apoptotic cells / Keith B. Elkon and Gregg J. Silverman -- Naturally occurring antibodies directed against carbohydrate tumor antigens / Reinhard Schwartz-Albiez -- Naturally occurring antibodies as therapeutics for neurologic disease : can human monoclonal IgMs replace the limited resource IVIG? / Arthur E. Warrington ... [et al.] -- Nature and nurture of catalytic antibodies / Sudhir Paul ... [et al.] -- Naturally occurring autoantibodies in mediating clearance of senescent red blood cells / Hans U. Lutz -- Naturally occurring autoantibodies against ss-amyloid / Jan-Philipp Bach and Richard Dodel -- Multi-faceted role of naturally occurring autoantibodies in fighting pathogens / Hicham Bouhlal and Srini Kaveri -- Immunoregulation by naturally occurring and disease-associated autoantibodies : binding to cytokines and their role in regulation of T-cell responses / Claus H. Nielsen and Klaus Bendtzen -- Modulation of dendritic cells and regulatory T cells by naturally occurring antibodies / Jaap Kwekkeboom -- Control of B cells expressing naturally occurring autoantibodies / Jean Louis Pasquali and Thierry Martin -- Granulocyte death regulation by naturally occurring autoantibodies / Stephan Von Gunten and Hans-Uwe Simon -- Naturally occurring autoantibodies mediate ischemia/reperfusion-induced tissue injury / Sherry D. Fleming -- How immune complexes from certain IgG NAbs and any F(ab')2 can mediate excessive complement activation / Hans U. Lutz -- The natural autoantibody repertoire in newborns and adults : a current overview / Asaf Madi ... [et al.] -- Antibody polyspecificity : what does it matter? / Jordan D. Dimitrov, Anastas D. Pashov, Tchavdar L. Vassilev -- Positive and negative selection of natural autoreactive B cells / Richard R. Hardy and Kyoko Hayakawa -- Naturally occurring antibodies/autoantibodies in polyclonal immunoglobulin concentrates / Peter J. Späth and Hans U. Lutz.
    Also available: Print – 2012
  • 2015 Springer
    Gregory Lloyd Blatch, Adrienne Lesley Edkins, editors.
  • 2008 Springer
    edited by Michael S. Ritsner and Abraham Weizman.
  • 2011 Springer
    Gianluca Gallo, Lorene M. Lanier, editors.
    1. Introduction to the neurobiology of actin -- 2. The neuronal actin cytoskeleton and the protrusion of lamellipodia and filopodia -- 3. Regulation of actin filaments during neurite extension and guidance -- 4. Functions of myosin motor proteins in the nervous system -- 5. Microtubule-actin interactions during neuronal development -- 6. Working with actin: methodological approaches for the study of actin in neurons -- 7. Functions of the actin cytoskeleton in the early embryology of the nervous system -- 8. Actin cytoskeletal regulation in neuronal migration -- 9. Actin and neuronal polarity -- 10. Actin at the synapse: contribution to pre- and postsynaptic functions -- 11. Actin and diseases of the nervous system -- 12. Signal transduction pathways: from receptor to the actin cytoskeleton -- Index.
  • 2015 Springer
    Caroline Fonta, László Négyessy.
    Phosphatases such as TNAP are fundamental in regulating cellular, and consequently numerous body functions. TNAP is a ubiquitous enzyme with a wide spectrum of substrates and specificity regulation at the cellular level and the lack of TNAP activity is a lethal condition. Recent findings of a highly specific regional, laminar and subcellular localization of TNAP in the cerebral cortex indicates that in addition to its metabolic and skeletal functions, TNAP also plays a role in regulating cerebral functions, most probably cognition. In fact, TNAP disturbance could result in complex diseases such as epilepsy, developmental retardation and Alzheimer disease. Available data suggest that, regarding brain functions, TNAP is a potentially important target of clinical research. The proposed book aims to provide an overview of our current understanding of the functions of TNAP in the brain.
  • 2010 Springer
    edited by Timothy G. Geary, Aaron G. Maule.
    Receptor-based discovery strategies for insecticides and parasiticides: a review / Debra Woods ... [et al.] -- Nonpeptide ligands for peptidergic G protein-coupled receptors / Timothy G. Geary -- Interaction of mimetic analogs of insect kinin neuropeptides with arthropod receptors / Ronald J. Nachman and Patricia V. Pietrantonio -- Neuropeptides in helminths: occurrence and distribution / Nikki J. Marks and Aaron G. Maule -- Neuropeptide physiology in helminths / Angela Mousley ... [et al.] -- Neuropeptide gene families in Caenorhabditis elegans / Chris Li and Kyuhyung Kim -- Control of nematode parasites with agents acting on neuromusculature systems: lessons for neuropeptide ligand discovery / Richard J. Martin and Alan P. Robertson -- Neuropeptide signaling in insects / Miriam Altstein and Dick R. Nassel -- Neuropeptide physiology in insects / William G. Bendena -- Neuropeptide biology in Drosophila / Elke Clynen ... [et al.] -- Neuropeptide receptors as possible targets for development of insect pest control agents / Matthias B. Van Hiel ... [et al.].
    Also available: Print – 2010
  • 2012 CRCnetBASE
    edited by Fred J. Nyberg.
    "Preface: Over the past four decades, the number of peptides identified as neurotransmitters or neuromodulators in the central and peripheral nervous systems has significantly increased. These compounds, known as neuropeptides, have been recognized for playing an important role in the communication between cells in a variety of neuronal networks. Although, a considerable number of neuropeptides have been characterized so far, their quantity is limited compared to the number of precursor proteins that are actually found to be expressed in cells of the nervous system. Numerous studies have confirmed that neuropeptides are involved in a number of biological activities. These include modulation of brain reward, pain processing, and immune response, as well as neuroendocrine regulations, control of neurovegetative functions, and trophic effects. In recent years, it has become evident that the role of neuropeptides as fast-acting neurotransmitters is challenged by the observation that many of them may act as growth factors by stimulating cell proliferation and slow-acting mitogenesis. It has thus been demonstrated that a number of neuroactive peptides, such as pituitary adenylate cyclase-activating polypeptide (PACAP), adrenocorticotrophin (ACTH), opioid peptides, somatostatin, and substance P, may modulate proliferation and cell viability. Some peptides act as stimulatory factors on brain circuits involved in cognition, whereas others may act as inhibitory factors. Accordingly, many neuropeptide systems appear as important targets for neuroprotective drugs and drugs that promote neuroregeneration"--Provided by publisher.
  • 2011 Springer Protocols
    edited by Ka Wan Li.
    Neuroproteomics : deciphering brain function and disorders / Ka Wan Li -- Dissection of rodent brain regions / Sabine Spijker -- Subcellular fractionation of brain tissue using free-flow electrophoresis / Markus Islinger ... [et al.] -- Isolation of synapse subdomains by subcellular fractionation using sucrose density gradient centrifugation / Tatsuo Suzuki -- Enrichment of plasma membranes from small brain samples by aqueous polymer two-phase systems / Jens Schindler -- Identification and characterization of protein complexes from total brain and synaptoneurosomes : heterogeneity of molecular complexes in distinct subcellular domains / Silvia De Rubeis and Claudia Bagni -- Analysis of protein complexes by 2d blue native/SDS-PAGE and antibody-shift assay / Dong Yang ... [et al.] -- Two-dimensional gel electrophoresis-based proteomic analysis of brain synapses / Karl-Heinz Smalla and Ursula Wyneken -- Two-dimensional BAC-SDS polyacrylamide gel electrophoresis for the fractionation and identification of synaptic vesicle proteins and the presynaptic active zone / Joern Barth and Walter Volknandt -- iTRAQ-based LC-LC MALDI TOF/TOF MS quantitative analysis of membrane proteins from human glioma / Uros Rajcevic -- OFFGEL-isoelectric focusing fractionation for the analysis of complex proteome / Emilie Ernoult and Catherine Guette -- 1D-PAGE/LC-ESI linear ion trap orbitrap MS approach for the analysis of synapse proteomes and synaptic protein complexes / Ning Chen, Roel C. vd Schors, and August B. Smit -- SDS-PAGE immunoblot analysis / Patricia Klemmer -- Phosphoproteomics by highly selective IMAC protocol / Chia-Feng Tsai ... [et al.] -- Global analysis of ubiquitination / David Meierhofer and Peter Kaiser -- High-throughput high-content functional image analysis of neuronal proteins implicated in Parkinson's disease / Eva Blaas and Ronald E. van Kesteren -- Neuropeptidomics of the mammalian brain / Fang Xie, Elena V. Romanova, and Jonathan V. Sweedler -- In-depth analysis of the cerebrospinal fluid proteome and biomarker discovery : abundant protein depletion sample pretreatment method / Silvina A. Fratantoni and Connie R. Jimenez -- Lipidomics of the nervous system : phospholipidomics by liquid chromatography coupled to mass spectrometry or tandem mass spectrometry / Su Chen -- Bioinformatics procedures for analysis of quantitative proteomics experiments using iTRAQ / Pim van Nierop and Maarten Loos -- Statistical analysis of spectral count data generated by label-free tandem mass spectrometry-based proteomics / Thang V. Pham and Connie R. Jimenez.
  • 2016 Springer
    Ulrich Nielsch, Ulrike Fuhrmann, Stefan Jaroch, editors.
    This volume gives an overview of state of the art technologies and future developments in the field of preclinical pharmaceutical research. A balanced mix of experts from academia and industry give insight in selected new developments in the drug discovery pathway. The topics cover the different parts of the drug discovery process, starting with new developments in the target identification and validation area. The lead generation part as a next step focuses on the requirements and technologies to identify new small molecules as lead compounds for further optimization; in a second section the technologies to identify biologics as leads are addressed. The final part focuses on the pharmacological models and technologies to characterize new compounds and the impact of biomarkers to facilitate the transfer of drug candidates into the development phase.
  • 2012 Springer
    Avi Ma'ayan, Ben D. MacArthur, editors.
    Gene Expression Profiling and Regulatory Networks in Single Cells / Fumio Arai, Kentaro Hosokawa, Yoshiko Matsumoto, Hirofumi Toyama and Toshio Suda -- Cell Fate Regulatory Networks / Sonya J. Ridden and Ben D. MacArthur -- Noise in Regulatory Networks / Srinandan Dasmahapatra -- Generalised Modelling in Systems Biology / Thilo Gross -- Relative Quantification Mass Spectrometry Using iTRAQ Isobaric Tags / Richard D. Unwin, Emma Carrick and Anthony D. Whetton -- Deciphering Protein Complexes and Protein Interaction Networks for Stem Cell Pluripotency / Jianlong Wang -- Omics Era in Stem Cell Research: Data Integration of Multi-regulatory Layers / Ana Sevilla, Benjamin Trinite and Ihor R. Lemischka -- High Throughput Affinity Purification and Mass Spectrometry to Determine Protein Complex Interactions / Anna Malovannaya, Rainer B. Lanz, Bert W. O'Malley and Jun Qin -- Network Pharmacology to Aid the Drug Discovery Process / Ruth Dannenfelser, Huilei Xu, Catherine Raimond and Avi Ma'ayan -- What Can Networks Do for You? / Bernhard M. Schuldt, Franz-Josef Müller and Andreas A. Schuppert.
  • 2007 Springer
    Yoshiki Nishizawa, Hirotoshi Morii, and Jean Durlach, eds.
    Overview -- Magnesium homeostasis -- Nutrition -- Epidemiology -- Exercise -- Metabolic syndrome -- Cardiovascular disease -- Skeletal diseases and calcium metabolism -- Kidney -- Neurology -- Magnesium in dental medicine -- Magnesium in neurology and psychiatry -- Veterinary medicine.
  • 2006 Springer
    Hsiou-Chi Liou [editor].
  • 2010 ScienceDirect
    [edited by] Louise [i.e. Louis] J. Ignarro.
    Section I: -- Chemical biology: Determinants of nitric oxide chemistry: inpact of cell signaling processes / Douglas D. Thomas, Wilmarie Flores-Santana, Christopher H. Switzer -- Nitric oxide redox biochemistry in lipid environments / Andrés Trostchansky, Matías N. Möller, Silvina Bartesaghi, Horacio Botti, Ana Denicola, Rafael Radi and Homero Rubbo -- Mechanisms and biological consequences of peroxynitrite-dependent protein oxidation and nitration / Madia Trujillo, Beatriz Alvarez, José M. Souza, Natalia Romero, Laura Castro, Leonor Thomson and Rafael Radi -- Systems approaches to unraveling nitric oxide response netwoeks in prokaryotes / Laura R. Jarboe, Daniel R. Hyduke and James C. Liao. Section II: -- Principles of biology: (A. Nitric oxide synthases) Uncoupling of endothelial nitric oxide synthase in cardiovascular disease and its pharmacological reversal / Ulrich Förstermann -- Tetrahydrobiopterin: an essential cofactor for nitric oxide syntahases and amino acid hydroxylases / Christina E. Higgins and Steven S. Gross -- Regulation of the expression of inducible nitric oxide synthase / Hartmut Kleinert, Julia Art and Andrea Pautz -- Molecular regulation of inducible nitric oxide synthase / Qiang Du and David A. Geller -- (B. Guanylate cyclase and cyclic GMP) Soluble guanylate cyclase: allosteric activtion and redox regulation / Adrian J. Hobbs and Johannes-Peter Stasch (C. Nitric oxide signaling) Untargeted discovery of nitric oxide-modified proteins / Ruba Deeb, Tal Nuriel and Steven S. Gross -- Fatty acid transduction of nitric oxide signaling: cyclooxygenases, lipoxygenases and nitro-fatty acids / Marsha P. Cole and Bruce A. Freeman -- Nitric oxide signaling in vascular cells is regulated through CD47 by thrombospondin-1 / Jeffery S. Isenberg, William A. Frazier and David D. Roberts -- The regulation of cell energetics and mitochondrial signaling by nitric oxide / Alberto Boveris, Maria Cecilia Carreras and Juan Jose Poderoso -- (D. Regulation of nitric oxide production) Nitric oxide-asymmetric dimedthylarginine system in endothelial cell senescence / Fortunato Scalera and Stefanie M. Bode-Böger -- (E. Nitric oxide and remodeling) The role of nitric oxide in apoptosis and autophagy: biochemical and computational studies / P. A. Loughran, E. Z. Bagci, R. Zamora, Y. Vodovotz and T. R. Billiar -- (F. Nitrite and nitrate) Nitric oxide formation from inorganic nitrate and nitrite / Jon O. Lundberg and Eddie Weitzberg -- Mechanisms of nitrite reduction in ischemia in the cardiovascular system / Andrew Webb and Amrita Ahluwalia -- Nitrite therapy for ischemic syndromes / Christopher G. Kevil and David J. Lefer -- Nitrite and heme globins: reaction mechanisms and physiological targets / Sruti Shiva, Sheila Frizzill and Mark T. Gladwin. Section III: -- Principles of pathobiology (A. Nitric oxide and cardiovascular function) Nitric oxide in vascular damage and regeneration / Claudio Napoli, Lilach O. Lerman, Maria Luisa Balestrieri and Louis J. Ignarro -- Free radicals as atherosclerotic risk in relation to nitric oxide / Toshio Hayashi and Akihisa -- The role of oxidative stress in endothelial dysfunction and vascular inflammation / Elena Osto and Francesco Cosentino -- (B. Pathophysiological mechanism of nitric oxide) Nitric oxide, oxidative stress, immune response and critical care / Claudio Napoli, Ettore Crimi, Sharon Williams-Ignarro, Filomena de Nigris and Louis J. Ignarro -- Reactive metabolites of oxygen and nitrogen in liver ischemia and reperfusion injury / Ian N. Hines and Matthew B. Grisham -- Nitric oxide in airway inflammation / Yong Chul Lee, So Ri Kim, Eun-Kyeong Jo, Hyun-Ock Pae and Hun-Taeg Chung -- Novel therapeutic applications of nitric oxide in the inhibition of tumor malignancy and reversal of resistance / Benajmin Bonavida.
  • 2011 Springer Protocols
    edited by Helen O. McCarthy, Jonathan A. Coulter.
    Nitric oxide physiology and pathology / David G. Hirst and Tracy Robson -- Ionizing radiation-induced DNA strand breaks and [gamma]-H2AX foci in cells exposed to nitric oxide / Kai Rothkamm and Susanne Burdak-Rothkamm -- Determination of S-nitrosothiols in biological fluids by chemiluminescence / Enika Nagababu and Joseph M. Rifkind -- Measurement of nitrite in blood samples using the ferricyanide-based hemoglobin oxidation assay / Barbora Piknova and Alan N. Schechter -- Selective fluorescent activation for bioimaging the expression of nitric oxide in cellular and in vivo systems / Junfeng Zhang and Hao Hong -- Real-time measurement of murine hippocampus NO levels in response to cerebral ischemia/reperfusion / Xiaoxiang Zheng, Kezhou Liu, and Yong Yang -- Detection of low levels of nitric oxide using an electrochemical sensor / Yong Chool Boo ... [et al.] -- Determination of the scavenging capacity against reactive nitrogen species by automatic flow injection-based methodologies / Marcela A. Segundo ... [et al.] -- Aqueous measurement of nitric oxide using membrane inlet mass spectrometry / David N. Silverman and Chingkuang Tu -- Quantum cascade laser technology for the ultrasensitive detection of low-level nitric oxide / Angela Elia ... [et al.] -- Determination of in vivo nitric oxide levels in animal tissues using a novel spin trapping technology / Anatoly F. Vanin and Alexander A. Timoshin -- [Beta] cell protection by inhibition of iNOS through lentiviral vector-based strategies / Sean O. Hynes, Cillian McCabe, and Timothy O'Brien -- Characterization of nitric oxide delivery systems produced by various nanotechnologies / Chi H. Lee -- Nitric oxide releasing nanoparticle synthesis and characterization / George Han, Adam J. Friedman, and Joel M. Friedman -- NOS antagonism using viral vectors as an experimental strategy : implications for in vivo studies of cardiovascular control and peripheral neuropathies / Beihui Liu ... [et al.]
  • pt. E-F, 2005-2008. ScienceDirect
    pt. F, 2008 ScienceDirect
    edited by Lester Packer, Enrique Cadenas.
  • 2008 ScienceDirect
    edited by Enrique Cadenas and Lester Packer.
    Also available: Print – 2008
  • 2011 Springer Protocols
    edited by Markus W. Ribbe.
    Historic overview of nitrogenase research / Yilin Hu and Markus W. Ribbe -- Mechanism of mo-dependent nitrogenase / Zhi-Yong Yang, Karamatullah Danyal, and Lance C. Seefeldt -- Assembly of nitrogenase MoFe protein / Chi-Chung Lee -- Genomic analysis of nitrogen fixation / Ina P. O'Carroll and Patricia C. Dos Santos -- Enzymatic systems with homology to nitrogenase / Jurgen Moser and Markus J. Brocker -- Molecular biology and genetic engineering in nitrogen fixation / Patricia C. Dos Santos -- Purification of nitrogenase proteins / Jared A. Wiig [and others] -- Assays of nitrogenase reaction products / William E. Newton and Michael J. Dilworth -- Methods for nitrogenase-like dark operative protochlorophyllide oxidoreductase / Jurgen Moser and Markus J. Brocker -- X-ray crystallography / Lauren E. Roth and F. Akif Tezcan -- X-ray absorption spectroscopy / Serena DeBeer -- Small angle x-ray scattering spectroscopy / David W. Mulder and John W. Peters -- Electron paramagnetic resonance spectroscopy / Karamatullah Danyal, Zhi-Yong Yang, and Lance C. Seefeldt -- Magnetic circular dichroism spectroscopy / Brian J. Hales -- Mössbauer spectroscopy / Boi Hanh Huynh -- Protocols for cofactor isolation of nitrogenase / Aaron W. Fay [and others] -- Techniques for functional and structural modeling of nitrogenase / Patrick L. Holland -- Nitrogenase structure and function relationships by density functional theory / Travis V. Harris and Robert K. Szilagyi -- Modeling the MoFe nitrogenase system with broken symmetry density functional theory / Gregory M. Sandala and Louis Noodleman.
  • 2014 Springer
    Muller Fabbri, editor.
    Introduction / Muller Gabbri -- Biogenesis and physiology of microRNAs / Carlos A. Melo and Sonia A. Melo -- Detecting noncoding RNA expression : from arrays to next-generation sequencing / Catalina Perdomo, Joshua Campbell, and Frank Schembri -- MicroRNAs in solid tumors / Jin Wang and Subrata Sen -- MicroRNAs in hematologic malignancies / Johannes Bloehdorn ... [et al.] -- miRNAs as cancer biomarkers / Edward R. Sauter -- Unraveling the complex network of interactions between noncoding RNAs and epigenetics in cancer / Veronica Davalos and Manel Esteller -- MicroRNA SNPs in cancer / Yujing LI and Peng Jin -- Bioinformatics approaches to the study of MircoRNAs / Ravi Gupta and Ramana V. Davuluri -- Beyond miRNAs : role of other noncoding RNAs in cancer / Roxana S. Redis and George A. Calin -- Translational implication for noncoding RNA in cancer / Duaa Dakhallah, Melissa Piper, and S. Patrick Nana-Sinkam.
  • 2012 Springer
    Farid Rahimi, Gal Bitan, editors.
  • 2009 ScienceDirect
    edited by Tom W. Muir, John N. Abelson.
    Also available: Print – 2009
  • 2012 Springer
    Inge Brouns ... [et al.].
  • 2015 Springer Protocols
    edited by Bruce G. Allan, Terence E. Hébert
    Studies of intracellular angiotensin II / Richard N. Re and Julia L. Cook -- Single-cell microinjection coupled to confocal microscopy to characterize nuclear membrane receptors in freshly isolated cardiomyocytes / Clémence Merlen and Jonathan Ledoux -- Design and application of light-activated probes for cellular signaling / David Chatenet, Steve Bourgault, and Alain Fournier -- Using caged ligands to study intracrine endothelin signaling in intact cardiac myocytes / Clémence Merlen, Louis R. Villeneuve, and Bruce G. Allen -- Quantification of catecholamine uptake in adult cardiac myocytes / Erika F. Dahl, Casey D. Wright, and Timothy D. O'Connell -- Characterization of the interaction between the prostaglandin D2 DP1 receptor and the intracellular L-Prostaglandin D synthase / Chantal Binda and Jean-Luc Parent -- Isolation and study of cardiac nuclei from canine myocardium and adult ventricular myocytes / Artavazd Tadevosyan, Bruce G. Allen, and Stanley Nattel -- High resolution imaging and function of nuclear G protein-coupled receptors (GPCRs) / Vikrant K. Bhosle [and others] -- Biochemical fractionation of membrane receptors in the nucleus / Ying-Nai Wang ... [et at.] -- Functional G protein-coupled Receptors on nuclei from brain and primary cultured neurons / Yuh-Jiin I. Jong and Karen L. O'Malley -- Automated microscopy of cardiac myocyte hypertrophy : a case study on the role of intracellular [alpha]-adrenergic receptors / Karen A. Ryall and Jeffrey J. Saucerman -- Measuring intranuclear and nuclear envelope [Ca2+] vs. cytosolic [Ca2+] / Senka Ljubojević and Donald M. Bers -- Assessing GPCR and G protein signaling to the nucleus in live cells using fluorescent biosensors / Julie Bossuyt and Donald M. Bers -- Tandem affinity purification to identify cytosolic and nuclear G [beta-gamma]-interacting proteins / Rhiannon Campden [and others] -- Examining the effects of nuclear GPCRs on gene expression using isolated nuclei / George Vaniotis [and others] -- Trafficking and function of GPCRs in the endosomal compartment / Davide Calebiro [and others].
  • 2005 Springer
    [edited by] Tzvi Tzfira, Vitaly Citovsky.
    Nuclear import and export of mammalian viruses / Michael Bukrinsky -- Nuclear import of DNA / David A. Dean and Kerimi E. Gokay -- Nuclear transport research methods / Jose Omar Bustamante. Regulation of nuclear import and export of proteins in plants and its role in light signal transduction / Stefan Kircher ... [et al.] -- Nuclear export: shuttling across the nuclear pore / John A. Hanover and Dona C. Love -- Nuclear protein import: distinct intracellular receptors for different types of import substrates / David A. Jans and Jade K. Forwood -- The molecular mechanisms of mRNA export / Tetsuya Taura, Mikiko C. Siomi and Haruhiko Siomi -- Structure of the nuclear pore / Michael Elbaum -- Integral proteins of the nuclear pore membrane / Merav Cohen, Katherine L. Wilson and Yosef Gruenbaum -- Subnuclear trafficking and the nuclear matrix / Iris Meier -- Nuclear import and export signals / Toshihiro Sekimoto, Jun Katahira and Yoshihiro Yoneda -- Nuclear import of plant proteins / Glenn R. Hicks -- Nuclear import of agrobacterium T-DNA / Tzvi Tzfira, Benoit Lacroix and Vitaly Citovsky --
  • 2002 ScienceDirect
    Vincent Laudet and Hinrich Gronemeyer.
  • 2003 ScienceDirect
    edited by David W. Russell, David J. Mangelsdorf.
    Also available: Print – 2003
  • 2010 Springer
    edited by Chris M. Bunce, Moray J. Campbell.
    Nuclear receptors an introductory overview / C.M. Bunce and M.J. Campbell -- What does evolution teach us about nuclear receptors? / G. Markov, F. Bonneton and V. Laudet -- Functions of nuclear receptors in insect development / D. Martín -- The glucocorticoid receptor / R.H. Oakley and J.A. Cidlowski -- Estrogen receptors: Their actions and functional roles in health and disease / S. Nilsson and J.-Å. Gustafsson -- Androgen receptor / J.T. Dalton and W. Gao -- Thyroid hormone receptors / B. Vennström, H. Liu and D. Forrest -- The vitamin D receptor (NR1I1) / O. Maguire and M.J. Campbell -- Retinoic acid receptors / A. Cras, F. Guidez and C. Chomienne -- PPARs: Important regulators in metabolism and inflammation / L.M. Sanderson and S. Kersten -- Xenobiotic receptors CAR and PXR / C. Klaassen and H. Lu -- Fxr / Y. Wang ... [et al.] -- Physiological functions of TR2 and TR4 orphan nuclear receptor / S. Liu ... [et al.] -- Nuclear receptors and ATP dependent chromatin remodeling: A complex story / C.J. Burd and T.K. Archer -- Non-genomic action of sex steroid hormones / A. Migliaccio, G. Castoria and F. Auricchio -- Ligand regulation and nuclear receptor action / M. Hewison -- New insights to nuclear receptor gene regulation from analysis of their response elements in target genes / C. Carlberg -- Systems biology: Towards realistic and useful models of molecular networks / F.J. Bruggeman ... [et al.].
  • 2016 Springer Protocols
    edited by Gunter Mayer.
    Selection of aptamers for metabolite sensing and construction of optical nanosensors / Yi Long ... [et al.] -- SELEX of cell-specific RNA aptamers / Katharina Berg, Eileen Magbanua, and Ulrich Hahn -- Developing aptamers by cell-based SELEX / Silvia Catuogno, Carla Lucia Esposito, and Vittorio de Franciscis -- DNA aptamer generation by genetic alphabet expansion SELEX (ExSELEX) using an unnatural base pair system / Michiko Kimoto, Ken-ichiro Matsunaga, and Ichiro Hirao -- Capillary electrophoresis for the selection of DNA aptamers recognizing activated protein C / Nasim Shahidi Hamedani and Jens Müller -- Preparation of SELEX samples for next-generation sequencing / Fabian Tolle and Günter Mayer -- Next-generation analysis of deep sequencing data : bringing light into the black box of SELEX experiments / Michael Blank -- Aptamer binding studies using MicroScale thermophoresis / Dennis Breitsprecher ... [et al.] -- Label-free determination of the dissociation constant of small molecule-aptamer interaction by isothermal titration calorimetry / Marc Vogel and Beatrix Suess -- Applications of aptamers in flow and imaging cytometry / Isis C. Nascimento ... [et al.] -- In vitro and in vivo imaging of fluorescent aptamers / Ioanna Théodorou ... [et al.] -- Crystallographic pursuit of a protein-RNA aptamer complex / John J. G. Tesmer -- Voltammetric aptasensor based on magnetic beads assay for detection of human activated protein C / Arzum Erdem, Gulsah Congur, and Ece Eksin -- Apta-PCR / Alessandro Pinto ... [et al.] -- Aptamer-based enzyme capture assay for measurement of plasma thrombin levels / Jens Müller ... [et al.] -- Application of aptamers in histopathology / Sarah Shigdar ... [et al.] -- Aptamer stainings for super-resolution microscopy / Maria Angela Gomes de Castro, Burkhard Rammner, and Felipe Opazo -- Synthesis and characterization of aptamer-targeted SNALPs for the delivery of siRNA / Samantha E. Wilner and Matthew Levy -- Screening of genetic switches based on the twister ribozyme motif / Michele Felletti, Benedikt Klauser, and Jörg S. Hartig.
  • 2014 Springer
    Jørgen Kjems, Elena Ferapontova, Kurt V. Gothelf, editors.
    This volume on nucleic acid nanotechnology offers authoritative, up-to-date and comprehensive coverage of nanotechnological studies and applications of nucleic acids. It provides reviews of various aspects of nucleic acid nanotechnology, each written by an internationally leading expert in the field, and presents state-of-the-art and recent advances in nucleic acid synthetic modifications, nanoscale design, manipulation and current and future applications in bioengineering, medicine, electronics, genetic analysis, chemistry, molecular biology, surface and material sciences. It examines how nucleic acid research is merging with nanotechnology, allowing the nanoscale properties of nucleic acid to be exploited in performing challenging nanotechnological tasks, from nanorobotics and nanosensing to nucleic acid computing. This book will above all benefit anyone who is interested in nanotechnological concepts of nucleic acid design and applications, and offers a valuable resource for teaching these concepts. It is essential reading for a broad audience of scientists both in academia and industry who wish to expand their expertise on the potential of nucleic acid functions and applications.
  • 2014 Springer
    Katsuhiko S. Murakami, Michael A. Trakselis, editors.
    This book provides a review of the multitude of nucleic acid polymerases, including DNA and RNA polymerases from Archea, Bacteria and Eukaryota, mitochondrial and viral polymerases, and other specialized polymerases such as telomerase, template-independent terminal nucleotidyl transferase and RNA self-replication ribozyme. Although many books cover several different types of polymerases, no book so far has attempted to catalog all nucleic acid polymerases. The goal of this book is to be the top reference work for postgraduate students, postdocs, and principle investigators who study polymerase.
  • 2011 Springer
    Mark O.J. Olson [editor].
  • 2005 CRCnetBASE
    [edited by] Morteza M. Vaghefi.
  • pt. A-B, 2012. ScienceDirect
    pt. B ScienceDirect
    edited by Carl Wu and C. David Allis.
  • pt. A-E, 1992-2004. ScienceDirect
    pt. B, 1994 ScienceDirect
    pt. C, 2000 ScienceDirect
    pt. D, 2004 ScienceDirect
    pt. E, 2004 ScienceDirect
    edited by Ludwig Brand, Michael L. Johnson.
  • 2011 Springer
    Heino Prinz.
  • 2009 CRCnetBASE
    [edited by] Sang-Woon Choi, Simonetta Friso.
    Methylation on the nucleosome / Da Jia and Xiaodong Cheng -- Mammalian DNA methyltransferases / Michael J. Boland and Judith K. Christman -- Chromatin remodeling and histone modifications / Gavin R. Schnitzler -- Nutrients and DNA methylation / Sang-Woon Choi, Roberto Corrocher, and Simonetta Friso -- Nutrients, histone modifications, and chromatin remodeling in chronic inflammation / Barbara Delage and Roderick H. Dashwood -- Nutrients, epigenetics, and embryonic development / Craig A. Cooney -- Nutrients, epigenetics, and aging / John C. Mathers and Dianne Ford -- Nutrition, epigenetics, and cancer / Sharon A. Ross -- Conclusions and future perspectives / Sang-Woon Choi and Simonetta Friso.
  • 2012 CRCnetBASE
    edited by Abhijit Ray, Punit Kumar Srivastava.
    "Incidences of inflammatory airway diseases are on the rise across the world. Existing therapeutic options are ineffective, unsafe, and expensive, and severe cases are nonresponsive to conventional therapy. Therefore, it is imperative that research be undertaken to discover new treatment options. Obstructive Airway Diseases: Role of Lipid Mediators discusses clinically successful and potential lipid targets that can make a difference in treating some of the most intractable disease states."--Publisher description.
  • 2008 Springer Protocols
    edited by Delphine Pflieger and Jean Rossier.
    Analysis of organelles by on-line two-dimensional liquid chromatography-tandem mass spectrometry / Edwin P. Romijn and John R. Yates III -- Purification and proteomic analysis of chloroplasts and their sub-organellar compartments / Daniel Salvi ... [et al.] -- Proteomic analysis of the stacked golgi complex / Mark S. Lowenthal, Kathryn E. Howell, and Christine C. Wu -- Purification of Saccharomyces cerevisiae mitochondria by zone electrophoresis in a free flow device / Hans Zischka ... [et al.] -- Preparation of respiratory chain complexes from Saccharomyces cerevisiae wild-type and mutant mitochondria : activity measurement and subunit composition analysis / Claire Lemaire and Geneviève Dujardin -- Mitochondrial proteomics : analysis of a whole mitochondrial extract with two-dimensional electrophoresis / Thierry Rabilloud -- Purification and proteomic analysis of the mouse liver mitochondrial inner membrane / Sandrine Da Cruz and Jean-Claude Martinou -- Subcellular fractionation and proteomics of nuclear envelopes / Laurence Florens, Nadia Korfali, and Eric C. Schirmer -- Purification and proteomic analysis of a nuclear-insoluble protein fraction / Tsuneyoshi Horigome, Kazuhiro Furukawa, and Kohei Ishii -- Preparation methods of human metaphase chromosomes for their proteome analysis / Kiichi Fukui, Hideaki Takata, and Susumu Uchiyama -- Purification and proteomic analysis of plant plasma membranes / Erik Alexandersson ... [et al.] -- Protocol to enrich and analyze plasma membrane proteins from frozen tissues / Jacek R. Wiśniewski -- Proteomic characterization of membrane protein topology / Adele R. Blackler and Christine C. Wu -- Free flow isoelectric focusing : a method for the separation of both hydrophilic and hydrophobic proteins of rat liver peroxisomes / Markus Islinger and Gerhard Weber -- Use of gas-phase fractionation to increase protein identifications : application to the peroxisome / Jacob Kennedy and Eugene C. Yi -- Purification and proteomic analysis of lysosomal integral membrane proteins / Huiwen Zhang ... [et al.] -- Affinity purification of soluble lysosomal proteins for mass spectrometric identification / Sylvie Kieffer-Jaquinod ... [et al.] -- Purification and proteomic analysis of synaptic vesicles / Holly D. Cox and Charles M. Thompson -- Purification and proteomics analysis of pancreatic zymogen granule membranes / Xuequn Chen and Philip C. Andrews -- Isolation and proteomic analysis of chlamydomonas centrioles / Lani C. Keller and Wallace F. Marshall -- Purification and proteomic analysis of 20S proteasomes from human cells / Marie-Pierre Bousquet-Dubouch ... [et al.] -- Characterization of E. coli ribosomal particles : combined analysis of whole proteins by mass spectrometry and of proteolytic digests by liquid chromatography-tandem mass spectrometry / Isabelle Iost ... [et al.] -- Assessment of organelle purity using antibodies and specific assays : the example of the chloroplast envelope / Daniel Salvi ... [et al.] -- Identifying bona fide components of an organelle by isotope-coded labeling of subcellular fractions : an example in peroxisomes / Marcello Marelli, Alexey I. Nesvizhskii, and John D. Aitchison -- Determination of genuine residents of plant endomembrane organelles using isotope tagging and multivariate statistics / Kathryn S. Lilley and Tom P.J. Dunkley -- Quantitative proteomic analysis to profile dynamic changes in the spatial distribution of cellular proteins / Wei Yan, Daehee Hwang, and Ruedi Aebersold -- Use of transcriptomic data to support organelle proteomic analysis / Wallace F. Marshall.
  • 2007 Springer
    William F. Martin, Miklós Müller, editors.
    The road to hydrogenosomes -- Mitochondria: key to complexity -- Origin, function,and transmission of mitochondria -- Mitochondria and their host: morphology to molecular phylogeny -- Anaerobic mitochondria: properties and origins -- Iron-sulfur proteins and iron-sulfur cluster assembly in organisms with hydrogenosomes and mitosomes -- Hydrogenosomes (and related organelles, either) are not the same -- The chimeric origin of mitochondria: photosynthetic cell enslavement, gene-transfer pressure, and compartmentation efficiency -- Constantin Merezhkowsky and the endokaryotic hypothesis -- The diversity of mitochondrion-related organelles amongst eukaryotic microbes -- Mitosomes of parasitic protozoa: biology and evolutionary significance -- index.
  • 2006 Springer
    Olivier Civelli, Qun-Yong Zhou (eds.).
    Also available: Print – 2008
  • 2010
    Erin Lynn Barnhart.
    Actin-based cell migration depends on the temporal and spatial integration of multiple force-generating systems. Polymerization of the actin network drives protrusion of the cell membrane, and the stretched membrane, in turn, exerts force on the actin network. Myosin contraction of the viscoelastic actin network is balanced by cell-substrate adhesion. The molecular components that contribute to each of these forces - actin-based protrusion, membrane tension, myosin-mediated contraction, and adhesion - have been extensively characterized, but the manner in which they are integrated in vivo to give rise to large-scale emergent properties such as cell shape and movement is not well understood. This thesis describes the role of cell-substrate adhesion in large-scale actin network organization and dynamic cell shape determination. Keratocytes exhibit several non-steady-state behaviors, including oscillatory retraction of the trailing edge, waves of protrusion at the leading edge, and symmetry breaking and motility initiation. I have shown that the mechanical and biochemical properties of adhesions contribute to each of these dynamic behaviors. Oscillatory retraction of the trailing edge emerges in fast-moving, fan-shaped keratocytes and I present evidence consistent with a mechanical model in which elastic coupling between the front of the cell and adhesions in the rear drives oscillations. In contrast, I have found that protrusion waves at the leading edge are likely the result of the biochemical, rather than mechanical, properties of adhesions. Traveling waves emerge at high adhesion strengths and adhesion maturation is required for waving. Mature adhesions have been shown previously to bind several components that contribute to leading edge protrusion, including VASP, a protein that promotes actin polymerization by preventing barbed end capping. Dynamic localization of VASP to the leading edge in waving keratocytes is consistent with a reaction-diffusion model in which global depletion of VASP by mature adhesion, in conjunction with local depletion by the polymerizing actin network, results in lateral propagation of protrusion waves. Finally, I present evidence that suggests that balance between cell-substrate adhesion and myosin contraction sets the mechanical instability threshold above which stationary, radially symmetric keratocytes break symmetry and begin to move. On the whole, the work described here represents significant progress towards elucidating the self-organization principles that underly whole cell migration.
  • 2007 ScienceDirect
    edited by Dieter Häussinger, Helmut Sies.
    Water compartments in cells / G. D. Fullerton ... [et al.] -- Yeast osmoregulation / S. Hohmann ... [et al.] -- Physiological analysis of bacterial mechanosensitive channels / I. R. Booth ... [et al.] -- Control of cell cycle in response to osmostress: Lessons from yeast / J. Clotet ... [et al.] -- Bacterial osmosensing transporters / J. M. Wood -- Plant gene networks in osmotic stress response: From genes to regulatory networks / L. S. Tran ... [et al.] -- Osmosensing by integrins in rat liver / F. Schliess ... [et al.] -- Hyperosmotic activation of the CD95 system / R. Reinehr ... [et al.] -- New approaches for determining apoptotic volume decrease in cells / C. D. Bortner ... [et al.] -- Transient receptor potential channels in mechanosensing and cell volume regulation / S. F. Pedersen ... [et al.] -- Cell volume regulatory ion channels in cell proliferation and cell death / F. Lang ... [et al.] -- Actin cytoskeleton architecture and signaling in osmosensing / E. A. Papakonstanti ... [et al.] -- Osmotic stress and DNA damage / N. I. Dmitrieva ... [et al.] -- Transcriptional activator tone-binding protein in cellular protection and differentiation / J. A. Kim ... [et al.] -- Desiccation response of mammalian cells: Anhydrosignaling / Z. Huang ... [et al.] -- Tonicity-regulated gene expression / J. D. Ferraris ... [et al.] -- Hyperosmotic induction of mitogen-activated protein kinase scaffolding / T. L. Hilder ... [et al.] -- Osmoregulation of bile formation / R. Kubitz ... [et al.] -- Osmosignaling and volume regulation in intestinal epithelial cells / C. H. Lim ... [et al.] -- Osmotic regulation of cellular glucose uptake / P. Gual ... [et al.] -- Effects of osmolytes on protein folding and aggregation in cells / Z. Ignatova ... [et al.] -- Simulations of macromolecules in protective and denaturing osmolytes: Properties of mixed solvent systems and their effects on water and protein structure and dynamics / D. A. Beck ... [et al.] -- Application of the transfer model to understand how naturally occurring osmolytes affect protein stability / M. Auton ... [et al.] -- Mechanisms of high salinity tolerance in plants / N. Tuteja -- Phenotype of the taurine transporter knockout mouse / U. Warskulat ... [et al.] -- Molecular basis of osmolyte effects on protein and metabolites / J. Rosgen -- Methods of changing biopolymer volume fraction and cytoplasmic solute concentrations for in vivo biophysical studies / M. C. Konopka ... [et al.] -- Characterization of plant aquaporins / R. Kaldenhoff ... [et al.].
    Also available: Print – 2007
  • 2006 MyiLibrary
    Managing editor, R. Cammack ; General editors, T.K. Attwood ... [et al.].
    Also available: Print – 2006
  • 2007 Springer
    [edited by] Mark D. Evans, Marcus S. Cooke.
    Oxidatively generated damage to cellular DNA : mechanistic aspects / Jean Cadet ... [et al.] -- Chlorination and nitration of DNA and nucleic acid components / Clare L. Hawkins ... [et al.] -- Prevention of the mutagenicity and cytotoxicity of oxidized purine nucleotides / Yusaku Nakabeppu ... [et al.] -- Nucleotide incision repair : an alternative and ubiquitous pathway to handle oxidative DNA damage / Sophie Couvé-Privat ... [et al.] -- OGG1 : from structural analysis to the knockout mouse / Arne Klungland, Jon K. Laerdahl, and Torbjörn Rognes -- Processing of 3'-end modified DNA strand breaks induced by oxidative damage / Jason L. Parsons, Emma Boswell, and Grigory L. Dianov -- Oxidative damage and promoter function / David Mitchell and Rita Ghosh -- Oxidative DNA damage and telomere shortening / Torsten Richter and Thomas von Zglinicki -- Oxidative damage and repair in the mitochondrial genome / Lene Juel Rasmussen and Keshav K. Singh -- The role of oxidative damage to nucleic acids in the pathogenesis of neurological disease / V. Prakash Reddy ... [et al.] -- Nucleic acid oxidation and the pathogenesis of cardiovascular diseases / Maria Grazia Andreassi -- Oxidative DNA damage and carcinogenesis / Ryszard Olinski, Marek Foksinski, and Barbara Tudek -- The physiological and pathological roles of oxidative damage to DNA in relation to life stage / Alberto Izzotti -- Analysis of 8-hydroxy-2'-deoxyguanosine as a marker of oxidatively damaged DNA in relation to carcinogenesis and aging / H. Kasai ... [et al.] -- Oxidatively damaged DNA and inflammation / Peter C. Dedon and Marita C. Barth -- The role of antioxidants in the prevention of oxidative damage to nucleic acids / Peter Muller and Steffen Loft.
  • v. 6-8, 10-22, 29-, 2001-02, 2004-06, 2012- CRCnetBase
    Cadenas, Enrique; Packer, Lester.
    Also available: Print – v. 15, 2005.
  • 2013 Springer Protocols
    edited by Donald Armstrong, Dhruba J. Bharali.
    Nanoscale biosensor for detection of reactive oxygen species / Tarl W. Prow, Daniel Sundh, and Gerard A. Lutty -- Nanoparticles : toxicity, radicals, electron transfer, and antioxidants / Peter Kovacic and Ratnasamy Somanathan -- Encapsulation of antioxidants in gastrointestinal-resistant nanoparticulate carriers / Eliana B. Souto ... [et al.] -- Toxicity of novel nanosized rormulations used in medicine / Afaf El-Ansary ... [e tal.] -- Fullerenol nanoparticles : toxicity and antioxidant activity / Rade Injac, Matevz Prijatelj, and Borut Strukelj -- Hydrogen peroxide (H2O2) detection with nanoprobes for biological applications : a mini-review / Gwangseong Kim, Yong-Eun Koo Lee, and Raoul Kopelman -- Studying the oxidative stress paradigm in vitro : a theoretical and practical perspective / Martin J.D. Clift and Barbara Rothen-Rutishauser -- Evaluation of the effect of acute and subacute exposure to TiO2 nanoparticles on oxidative stress / Shichang Liu and Zhuo Yang -- Biomedical applications of nano-antioxidant / Geeta Watal ... [et al.] -- Lipid peroxidation due to in vitro and in vivo exposure of biological samples to nanoparticles / Anca Dinischiotu ... [et al.] -- Testing nanomaterial toxicity in unicellular eukaryotic algae and fish cell lines / Alexandra Kroll, Dana Kühnel, and Kristin Schirmer -- Gold nanoparticles and oxidative stress in the blue mussel, mytilus edulis / Sara Tedesco ... [et al.] -- Oxidative biomarkers to assess the nanoparticle-induced oxidative stress / Rama Narsimha Reddy Anreddy, Narsimha Reddy Yellu, and Krishna R. Devarakonda -- Vesicular antioxidants : role in age-related cerebral oxidative injury / Sibani Sarkar, Ardhendu Kumar Mandal, and Nirmalendu Das -- Methods for detection of oxidative stress and genotoxicity of engineered nanoparticles / Ashutosh Kumar, Vyom Sharma, and Alok Dhawan -- Size-dependent study of pulmonary responses to nano- sized iron and copper oxide nanoparticles / Rajiv Kumar and Dattatri K. Nagesha -- In vitro techniques to investigate the oxidative effects of quantum dots / Ridhima Juneja and Indrajit Roy -- Hybrid polymeric nanoparticles : potential candidate for ophthalmic delivery / Dhruba J. Bharali, Donald Armstrong, and Shaker A. Mousa -- Relevance of nanotechnology in modulating oxidative stress : an overview / Dhruba J. Bharali and Shaker A. Mousa.
  • 2013 Springer
    Ursula Jakob, Dana Reichmann, editors.
    Many physiological conditions such as host defense or aging and pathological conditions such as neurodegenerative diseases, and diabetes are associated with the accumulation of high levels of reactive oxygen species and reactive nitrogen species. This generates a condition called oxidative stress. Low levels of reactive oxygen species, however, which are continuously produced during aerobic metabolism, function as important signaling molecules, setting the metabolic pace of cells and regulating processes ranging from gene expression to apoptosis. For this book we would like to recruit the experts in the field of redox chemistry, bioinformatics and proteomics, redox signaling and oxidative stress biology to discuss how organisms achieve the appropriate redox balance, the mechanisms that lead to oxidative stress conditions and the physiological consequences that contribute to aging and disease.
  • ScienceDirect
    edited by Lester Packer, Alexander N. Glazer.
    Also available: Print – 1990
  • ScienceDirect
    edited by Lester Packer.
    Also available: Print – 1994
  • 2013 Springer
    Pierre Hainaut, Magali Olivier, Klas G. Wiman, editors.
    TP53: Coordinator of the Processes That Underlie the Hallmarks of Cancer / Pierre Hainaut -- The Inheritance of p53 / Lukasz F. Grochola, Jorge Zeron-Medina, Emmanouela Repapi, Alexander E. Finlayson and Ying Cai, et al. -- p53: Guardian of the Metabolome / Masha V. Poyurovsky and Carol Prives -- The p53 Family and Stem Cell Biology / Massimiliano Agostini, Alessandro Rufini, Edward T. W. Bampton, Francesca Bernassola and Gerry Melino, et al. -- Mutant p53-Driven Tumorigenesis / Tamara Terzian and Guillermina Lozano -- Humanised Mouse Models: Targeting the Murine p53 Locus with Human Sequences / Monica Hollstein and Yang Xu -- p53 Models for Mammary Carcinogenesis / Wolfgang Deppert and Genrich Tolstonog -- TP53 Somatic Mutations: Prognostic and Predictive Value in Human Cancers / Magali Olivier -- Assessing TP53 Status in Human Tumors: Lessons from Breast Cancer / Anita Langerød, Magali Olivier and Anne-Lise Børresen-Dale -- TP53 Germline Mutations: Genetics of Li-Fraumeni Syndrome / Doua Bakry and David Malkin -- Tp53 Gene Therapy for Cancer Treatment and Prevention / Robert E. Sobol, Yong-Song Guan, Long-Jiang Li, Wei-Wei Zhang and Zhaohui Peng, et al. -- Upstream Targets in the p53 Pathway / Anna R. McCarthy and Sonia Lain -- p53-Reactivating Molecules as Research Tools and Anticancer Drugs / Vera V. Grinkevich, Andreas Warnecke and Galina Selivanova -- Targeting Mutant p53 for Improved Cancer Therapy / Jinfeng Shen, Vladimir J. N. Bykov and Klas G. Wiman -- p53 Immunotherapy of Cancer / Hakim Echchannaoui and Matthias Theobald -- Designing p53 Trials: A Surgical Oncologists View / Daniela Kandioler, Sonja Kappel and Brigitte Wolf -- p53 in the Clinic: A Pathologist's View / Philippe Bertheau, Jean-François Fléjou, Yves Allory, Pascale Varlet and Sylvie Lantuejoul, et al. -- Genetic Counseling for TP53 Germline Mutations / Maria Isabel Waddington Achatz and Patricia Ashton-Prolla.
  • 2013 Springer Protocols
    edited by Sumitra Deb, Swati Palit Deb.
    Detecting and quantifying p53 isoforms at mRNA level in cell lines and tissues / Marie P. Khoury ... [et al.] -- Detecting p53 isoforms at protein level / Virginie Marcel ... [et al.] -- Autophagy, senescence, and apoptosis / Rachel W. Goehe ... [et al.] -- p53 and cell cycle effects after DNA damage / Emir Senturk and James J. Manfredi -- p53 ubiquitination and proteasomal degradation / Ian M. Love, Dingding Shi, and Steven R. Grossman -- Identification of p53 in mitochondria / Angelina V. Vaseva and Ute M. Moll -- Identification of novel mutant p53 interacting proteins by proteomic analysis / Sumitra Deb and Paul R. Graves -- Identification of small molecules affecting p53-MDM2/MDMX interaction by fluorescence polarization / Qi Zhang and Hua Lu -- Determine the effect of p53 on chemosensitivity / Emir Senturk and James J. Manfredi -- Measurement of chemosensitivity and growth rate in p53 expressing cells / Mahesh Ramamoorthy ... [et al.] -- Mutant p53 in cell adhesion and motility / W. Andrew Yeudall, Katharine H. Wrighton, and Sumitra Deb -- Use of the DNA fiber spreading technique to detect the effects of mutant p53 on DNA replication / Rebecca A. Frum, Sumitra Deb, and Swati Palit Deb -- Generation of p53-deficient induced pluripotent stem cells from mouse embryo fibroblasts / Evguenia M. Alexandrova and Ute M. Moll -- p53 actions on microRNA expression and maturation pathway / Hiroshi I. Suzuki and Kohei Miyazono -- Isolation and characterization of murine multipotent lung stem cells / Venkat S. Gadepalli, Catherine Vaughan, and Raj R. Rao -- Generation of p53 knock-down cell lines / Catherine Vaughan, Swati Palit Deb, and Sumitra Deb -- ChIP for identification of p53 responsive DNA promoters / Jun-Ming Liao and Hua Lu -- ChIP-on-chip to identify mutant p53 targets / Frauke Goeman, Giulia Fontemaggi, and Giovanni Blandino -- ChIP sequencing to identify p53 targets / Catherine Vaughan, Brad Windle, and Sumitra Deb.
  • 2013 ScienceDirect
    edited by Hiroshi Maruta.
    1. Functional maturation of PAKs from unicellular to multicellular organisms / Masato Okada, Graham Cote, Ramesh K. Jha, and Hiroshi Maruta -- 2. Oncogenicity of PAKs and their substrates / Hong He and Hiroshi Maruta -- 3. Natural or synthetic therapeutics that block PAKs / Hiroshi Maruta, Shanta M. Messerli, and Ramesh K. Jha -- 4. PAK1-3 in infectious diseases / Hiroshi Maruta -- 5. PAK1 in brain diseases or disorders / Hiroshi Maruta and Shanta M. Messerli -- 6. PAK1 in Alzheimer's and Huntington's diseases / Qui-Lan Ma, Fusheng Yang, Sally A. Frautschy, and Greg M. Cole -- 7. PAK1 controls the lifespan / Sumino Yamase and Hiroshi Maruta -- 8. 3D structure and physiological regulation of PAKs / Stefan Knapp.
  • 2008 Springer
    edited by Bharti Mackness ... [et al.].
  • 2010 Springer
    Srinivasa T. Reddy, editor.
    Also available: Print – 2010
  • 2013 CRCnetBASE
    edited by Tania Nolan, Stephen A. Bustin.
    "A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution"--Provided by publisher.
  • 2011 ClinicalKey
    Chris van der Walle [editor].
    The growing area of peptide and protein therapeutics research is of paramount importance to medical application and advancement. A needed reference for entry level researchers and researchers working in interdisciplinary. Covering cerebral delivery, pulmonary delivery, transdermal delivery, intestinal delivery, ocular delivery, parenteral delivery, and nasal delivery, this resource offers an overview of the main routes in therapeutics. Researchers across biochemistry, pharmaceutical, molecular biology, cell biology, immunology, chemistry and biotechnology fields will find this publication invaluable for peptide and protein laboratory research. Covers current and emerging routes for delivery of therapeutics with a focus on end application Covers Comparative Effectiveness Research Concise, accessible information from active researchers in academia and industry.
  • 2015 Springer Protocols
    edited by Gunnar Houen.
    Peptide Antibodies: Past, Present, and Future / Gunnar Houen -- Structure of natural and recombinant antibodies / Hui Ma and Richard O'Kennedy -- Prediction of antigenic B and T cell epitopes via energy decomposition analysis : description of the web-based prediction tool BEPPE / Claudio Peri ... [et al.] -- Prediction of antibody epitopes / Morten Nielsen and Paolo Marcatili -- Fmoc solid-phase peptide synthesis / Paul R. Hansen and Alberto Oddo -- Peptide-carrier conjugation / Paul R. Hansen -- Solid-phase peptide-carrier conjugation / Gunnar Houen and Dorthe T. Olsen -- Analysis of peptides and conjugates by amino acid analysis / Peter Højrup -- Characterization of synthetic peptides by mass spectrometry / Bala K. Prabhala ... [et al.] -- Interpretation of tandem mass spectrometry (MSMS) spectra for peptide analysis / Karin Hjernø and Peter Højrup -- Polyclonal Peptide Antisera / Tina H. Pihl, Kristin E. Illigen, and Gunnar Houen -- Production and screening of monoclonal peptide antibodies / Nicole Hartwig Trier ... [et al.] -- Production of epitope-specific antibodies by immunization with synthetic epitope peptide formulated with CpG-DNA- liposome complex without carriers / Dongbum Kim, Younghee Lee, and Hyung-Joo Kwon -- Thioredoxin-displayed multipeptide immunogens / Angelo Bolchi ... [et al.] -- Purification of natural and recombinant peptide antibodies by affinity chromatographic strategies / Hui Ma and Richard O'Kennedy -- Isolation of camelid single-domain antibodies against native proteins using recombinant multivalent peptide ligands / Norah A. Alturki ... [et al.] -- Generation of TCR-Like antibodies using phage display / Brian H. Santich ... [et al.] -- Structural characterization of peptide antibodies/ Anna Chailyan and Paolo Marcatili -- Automated high-throughput mapping of linear B-cell epitopes using a statistical analysis of high-density peptide microarray data / Thomas Østerbye and Søren Buus -- Characterization of peptide antibodies by epitope mapping using resin-bound and soluble peptides / Nicole Hartwig Trier -- Screening and characterization of linear B-cell epitopes by biotinylated peptide libraries / Ida Rosenkrands and Anja Olsen -- Bead-based peptide arrays for profiling the specificity of modification state-specific antibodies / Angela Filomena ... [et al.] -- Surface plasmon resonance method to evaluate anti-citrullinated protein/peptide antibody affinity to citrullinated peptides / Feliciana Real-Fernández ... [et al.] -- Specificity analysis of histone modification-specific antibodies or reading domains on histone peptide arrays / Goran Kungulovski ... [et al.] -- Prion-specific antibodies produced in wild-type mice / Peter M. H. Heegaard ... [et al.] -- Immunoblotting with peptide antibodies : differential immunoreactivities caused by certain amino acid substitutions in a short peptide and possible effects of differential refolding of the peptide on a nitrocellulose or PVDF membrane / Takenori Yamamoto ... [et al.] -- Immunocytochemical and immunohistochemical staining with peptide antibodies / Tina Friis ... [et al.] -- Designing B-cell epitopes for immunotherapy and subunit vaccines / Harinder Singh ... [et al.] -- Enterovirus-specific anti-peptide antibodies / Chit Laa Poh ... [et al.] -- Therapeutic HIV peptide vaccine / Anders Fomsgaard.
  • 2007 Springer Protocols
    edited by Gregg B. Fields.
    HPLC analysis and purification of peptides / Colin T. Mant ... [et al.] -- Identification of proteins based on MS/MS spectra and location of posttranslational modifications / Kathryn L. Stone ... [et al.] -- Determination of phosphorylated and O-glycosylated sites by chemical targeting (CTID) at ambient temperature / Gary M. Hathaway -- Synthesis, biosynthesis, and characterization of transmembrane domains of a G protein-coupled receptor / Fred Naider -- Application of topologically constrained mini-proteins as ligands, substrates, and inhibitors / Janelle L. Lauer-Fields ... [et al.] -- Proteolytic profiling of the extracellular matrix degradome / Diane Baronas-Lowell ... [et al.] -- [Beta]-amyloid protein aggregation / Marcus A. Etienne ... [et al.] -- Radiometal-labeled somatostatin analogs for applications in cancer imaging and therapy / Jason S. Lewis and Carolyn J. Anderson -- Cell-penetrating proline-rich peptidomimetics / Josep Farrera-Sinfreu ... [et al.] -- Peptide-mediated targeting of liposomes to tumor cells / Evonne M. Rezler ... [et al.] -- Peptide-mediated delivery of nucleic acids into mammalian cells / Sébastien Deshayes ... [et al.] -- Broth microdilution antibacterial assay of peptides / Laszlo Otvos and Mare Cudic -- Depsipeptide synthesis / Maciej Stawikowski and Predrag Cudic.
  • 2015 Springer Protocols
    edited by Ratmir Derda.
    Design, synthesis, and application of OB2c combinatorial peptide and peptidomimetic libraries -- Synthesis of macrocyclic organo-peptide hybrids from ribosomal polypeptide precursors via cuAAC-/hydrazide-mediated cyclization -- Synthesis and screening of one-bead-one-compound cyclic peptide libraries -- Creating site-specific isopeptide linkages between proteins with the traceless staudinger ligation -- Macrocyclic templates for library synthesis of peptido-conjugates -- Synthesis and alkylation of aza-gly-pro building blocks of peptidomimetic libraries for developing prostaglandin f2alpha receptor modulators as therapeutics to inhibit preterm labor -- A fragment-based selection approach for the discovery of peptide macrocycles targeting protein kinases -- Peptide and peptide library cyclization via bromomethylbenzene derivatives -- Phage selection of bicyclic peptides based on two disulfide bridges -- De novo discovery of bioactive cyclic peptides using bacterial display and flow cytometry -- Chemical posttranslational modification of phage-displayed peptides -- Mapping protein-protein interactions with phage-displayed combinatorial peptide libraries and alanine scanning -- Identifying reactive peptides from phage-displayed libraries -- The best and the brightest: Exploiting tryptophan-sensitized tb(3+) luminescence to engineer lanthanide-binding tags -- Synthesis and cell-based screening of one-bead-one-compound peptide libraries -- Screening peptide array library for the identification of cancer cell-binding peptides -- Next-generation sequencing of phage-displayed peptide libraries -- Maleimide-based method for elaboration of cysteine-containing peptide phage libraries.
  • 2015 Springer Protocols
    editors Marina Cretich, Marcella Chiari.
    Peptide arrays on planar supports / Víctor Tapia Mancilla and Rudolf Volkmer -- High-throughput microarray incubations using multi-well chambers / Johannes Zerweck ... [et al.] -- Analysis of protein tyrosine kinase specificity using positional scanning peptide microarrays / Yang Deng and Benjamin E. Turk -- Secondary structure determination of peptides and proteins after immobilization / Stella H. North and Chris R. Taitt -- Peptides and anti-peptide antibodies for small and medium scale peptide and anti-peptide affinity microarrays : antigenic peptide selection, immobilization, and processing / Fan Zhang, Andrea Briones, and Mikhail Soloviev -- Low-cost peptide microarrays for mapping continuous antibody epitopes / Ryan McBride ... [et al.] -- Peptide microarray-based resonance light scattering assay for sensitively detecting intracellular kinase activity / Tao Li ... [et al.] -- Anomalous reflection of gold : a novel platform for biochips / Amir Syahir ... [et al.] -- High-throughput peptide screening on a bimodal imprinting chip through MS-SPRi integration / Weizhi Wang, Qiaojun Fang, and Zhiyuan Hu -- Analyzing peptide microarray data with the R pepStat package / Gregory Imholte, Renan Sauteraud, and Raphael Gottardo -- Chemoselective strategies to peptide and protein bioprobes immobilization on microarray surfaces / Alessandro Gori and Renato Longhi -- Manufacturing of peptide microarrays based on catalyst- free click chemistry / Denis Prim and Marc E. Pfeifer -- Clickable polymeric coating for oriented peptide immobilization / Laura Sola ... [et al.] -- Oriented peptide immobilization on microspheres / Lisa C. Shriver-Lake, George P. Anderson, and Chris R. Taitt -- Cell microarray format : a peptide release system using a photo-cleavable linker for cell toxicity and cell Uptake Analysis / Kenji Usui, Kin-ya Tomizaki, and Hisakazu Mihara -- Peptide microarrays for medical applications in autoimmunity, infection, and cancer / Carsten Grötzinger -- Synthetic peptide-based ELISA and ELISpot assay for identifying autoantibody epitopes / Judit Pozsgay ... [et al.] -- IgE and IgG4 epitope mapping of food allergens with a peptide microarray immunoassay / Javier Martínez-Botas and Belén de la Hoz -- IgE epitope mapping using peptide microarray immunoassay / Gustavo Gimenez, Sara Benedé, and Jing Lin -- Spot synthesis : an optimized microarray to detect IgE epitopes / Salvatore G. De-Simone, Paloma Napoleão-Pêgo, and Thatiane S. De-Simone -- Mapping of epitopes occurring in bovine αs1-casein variants by peptide microarray immunoassay / Maria Lisson and Georg Erhardt.
  • 2009 Springer Protocols
    edited by Marina Cretich, Marcella Chiari.
    Exploring and profiling protein function with peptide arrays / V.E. Tapia, B. Ay and R. Volkmer -- Peptide arrays for enzyme profiling / A. Thiele, J. Zerweck and M. Schutkowski -- Using peptide array to identify binding motifs and interaction networks for modular domains / S.S. Li and C. Wu -- Molecular simulations of peptides: a useful tool for the development of new drugs and for the study of molecular recognition / M. Meli and G. Colombo -- Synthesis of peptide arrays using SPOT-technology and the CelluSpots-method / D.F. Winkler [and others] -- Rapid identification of linear protein domain binding motifs using peptide SPOT arrays / D.J. Briant [and others] -- Characterization of kinase target phosphorylation consensus motifs using peptide SPOT arrays / G.C. Leung [and others] -- CelluSpots: a reproducible means of making peptide arrays for the determination of SH2 domain binding specificity / C. Wu and S.S. Li -- High-density peptide microarrays for reliable identification of phosphorylation sites and upstream kinases / A. Thiele [and others] -- Epitope mapping of human chromogranin A by peptide microarrays / M. Cretich [and others] -- Antimicrobial peptide arrays for detection of inactivated biothreat agents / C.R. Taitt, S.H. North and N.V. Kulagina -- Mapping functional prion-prion protein interaction sites using prion protein based peptide-arrays / A. Rigter [and others] -- A designed peptide chip: protein fingerprinting technology with a dry peptide array and statistical data mining / K. Usui, K.Y. Tomizaki and H. Mihara -- Peptide microarrays on bisphenol A polycarbonate / V. Souplet, C. Roux and O. Melnyk -- Self-assembly of PNA-encoded peptides into microarrays / F. Debaene and N. Winssinger -- A novel combinatorial approach to high-density peptide arrays / M. Beyer [and others] -- Polypyrrole-peptide microarray for biomolecular interaction analysis by SPR imaging / M.B. Villiers [and others] -- The peptide microarray-based assay for kinase functionality and inhibition study / Z. Wang -- An advanced application of protein microarrays: cell-based assays for functional genomics / R. Carbone -- Profiling the autoantibody repertoire by screening phage-displayed human cDNA libraries / R. Di Niro [and others] -- Visualisation and pre-processing of peptide microarray data / M. Reilly and D. Valentini -- Web-based design of peptide microarrays using microPepArray Pro / T. Li [and others] -- Qualitative and quantitative analysis of peptide microarray binding experiments using SVM-PEPARRAY / G. Chen [and others] -- PASE: a web-based platform for peptide/protein microarray experiments / F. Pamelard [and others].
  • 2013 Springer Protocols
    edited by Predrag Cudic.
    Hantzsch Based Macrocyclization Approach for the Synthesis of Thiazole Containing Cyclopeptides / Adel Nefzi -- The Chemical Synthesis of [alpha]-Conotoxins and Structurally Modified Analogs with Enhanced Biological Stability / Jayati Banerjee [and three other] -- Synthesis of AApeptides / Youhong Niu [and three others] -- Peptoids and Peptide-Peptoid Hybrid Biopolymers as Peptidomimetics / Maciej J. Stawikowski -- Synthesis of Side Chain N,N'-Diaminoalkylated Derivatives of Basic Amino Acids for Application in Solid-Phase Peptide Synthesis / Jean-Philippe Pitteloud, Nina Bionda, and Predrag Cudic -- Study Protein Folding and Aggregation Using Nonnatural Amino Acid p-Cyanophenylalanine as a Sensitive Optical Probe / Deguo Du, Haiyang Liu, and Bimlesh Ojha -- Adamantoylated Biologically Active Small Peptides and Glycopeptides Structurally Related to the Bacterial Peptidoglycan / Ruža Frkanec, Branka Vranešić, and Srdjanka Tomić -- Optimization of Physicochemical and Pharmacological Properties of Peptide Drugs by Glycosylation / Maria C. Rodriguez and Mare Cudic -- The Maillard Reaction Induced Modifications of Endogenous Opioid Peptide Enkephalin / Andreja Jakas -- Solid-Phase Guanidinylation of Peptidyl Amines Compatible with Standard Fmoc-Chemistry: Formation of Monosubstituted Guanidines -/ Nina Bionda and Predrag Cudic -- Stabilization of Collagen-Model,Triple-Helical Peptides for In Vitro and In Vivo Applications / Manishabrata Bhowmick and Gregg B. Fields -- Identification of Adipokine Receptor Agonists and Turning Them to Antagonists / Laszlo Otvos Jr. -- Peptide Detection and Structure Determination in Live Cells using Confocal Raman Microscopy / Andrew C. Terentis and Jing Ye.
  • 2013 Springer Protocols
    edited by Peter E. Nielsen, Daniel H. Appella.
    Exemplifying and illustrating recent exciting advances in PNA chemistry, the second edition of Peptide Nucleic Acids: Methods and Protocols serves as a vital complement to the first edition of the book. Since the discovery of peptide nucleic acids, many interesting new derivatives and analogues in terms of nucleic acid recognition specificity and affinity have emerged. Also, as this detailed volume presents, great ingenuity in exploiting the unique properties of PNAs for a wide variety of applications within drug discovery, medical diagnostics, chemical biology and nanotechnology has unfolded. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Peptide Nucleic Acids: Methods and Protocols, Second Editionserves as a source of useful specific methods and protocols as well as a source of inspiration for future developments.
  • 2006 Springer
    [edited by] Christopher G. Janson, Matthew J. During.
    PNAs as novel cancer therapeutics / Luca Mologni and Carlo Gambacorti-Passerini -- Medicinal chemistry of plasmid DNA with peptide nucleic acids : a new strategy for gene therapy / Olivier Zelphati ... [et al.] -- Locked nucleic acids (LNA) and medical applications / Henrik Ørum, Andreas Wolter and Lars Kongsbak -- Peptide nucleic acids as agents to modify target gene expression and function / Gan Wang and Peter M. Glazer -- Peptide nucleic acids : cellular delivery and recognition of DNA and RNA targets / David R. Corey -- Receptor-specific targeting with complementary peptide nucleic acids conjugated to peptide analogs and radionuclides / Eric Wickstrom, Mathew L. Thakur and Edward R. Sauter -- Morpholinos and PNAs compared / James E. Summerton -- Chemistry of locked nucleic acids (LNA) : design, synthesis and bio-physical properties / Jesper Wengel ... [et al.] -- Recent applications of RNA interference (RNAi) in mammalian systems / Lisa Scherer and John J. Rossi -- Peptide nucleic acids as epigenetic inhibitors of HIV-1 / Shizuko Sei -- Therapeutic uses of peptide nucleic acids (PNA) in oncology / Nadia Zaffaroni, Raffaella Villa and Marco Folini -- The many faces of PNA / Peter E. Nielsen -- Modulation of nucleic acid information processing by PNAs : potential use in anti-viral therapeutics / Lionel Bastide, Bernard Lebleu and Ian Robbins -- Targeted gene delivery : the role of peptide nucleic acid / Kenneth W. Liang, Feng Liu and Leaf Huang -- Imaging gene expression in the brain with peptide nucleic acid (PNA) antisense radiopharmaceuticals and drug targeting technology / Ruben J. Boado and William M. Pardridge -- The use of PNAs and their derivatives in mitochondrial gene therapy / Paul M. Smith ... [et al.] -- Gene silencing through RNA interference: potential for therapeutics and functional genomics / David O. Azorsa, Spyro Mousses and Natasha J. Caplen -- Transcriptional activation of human CREB gene promoter using bis-PNA (peptide nucleic acid) / Christopher G. Janson ... [et al.].
  • pt. 1-2, 2000-2003. ScienceDirect
    pt. 2 (2003) ScienceDirect
    editors, R. Quirion, A. Björklund, T. Hökfelt.
  • 2002 Springer Protocols
    edited by Janet J. Maguire and Anthony P. Davenport.
    Immunocytochemical localization of endothelin peptides, precursors, and endothelin-converting enzymes / R.E. Kuc -- Analysis of endothelins by enzyme-linked immunosorbent assay and radioimmunoassay / A.P. Davenport and R.E. Kuc -- Separation of endothelin isoforms by high performance liquid chromatography and detection by radioimmunoassay / M.J. Ashby -- Radioligand binding assays and quantitative autoradiography of endothelin receptors / A.P. Davenport and R.E. Kuc -- Using receptor antagonists in binding studies to characterize a mammalian endothelin receptor / J.R. Wu-Wong -- Quantification of endothelin receptor mrna by competitive rt-pcr / P.J. Smith and J.C. Monge -- Detection and assessment of endothelin-converting enzyme activity / C.D. Jackson and A.J. Turner -- Quantitative measurement of mrna levels by rt-pcr. Studies of ece-1 isoforms / D.M. Lees [and others] -- Evaluation of endothelin-converting enzyme inhibitors using cultured cells / R. Corder -- Endothelin-converting enzyme activity in vascular smooth muscle preparations in vitro / J.J. Maguire -- Characterization of a new endothelin receptor ligand by in vitro assays / J.R. Wu-Wong -- Use of in vitro organ cultures of human saphenous vein as a model for intimal proliferation / K.E. Porter -- Investigation of the endothelin system in experimental heart failure / G.A. Gray and L. Sherry -- Local forearm vasoconstriction to endothelin-1 measured by venous occlusion plethysmography in vivo / F.E. Strachan and D.J. Webb.
  • 2009 Springer
    Susan Del Valle, Emanuel Escher, William D. Lubell, editors.
    Also available: Print – 2009
  • 2013 Springer
    Jihan A. Youssef, Mostafa Z. Badr.
    Introduction -- History of PPAR Discovery -- Peroxisome Proliferator-Activated Receptors -- PPAR Ligands -- Tissue Distribution and Versatile Functions of PPARs -- PPARs and Drug Metabolism -- Molecular Aspects of PPAR Actions -- Animal Models in PPAR Research -- Safety of PPAR Agonists -- The Future of PPAR Research.
  • 2013 Springer Protocols
    edited by Mostafa Z. Badr, Jihan Youssef.
    PPARs : history and advances / Jihan Youssef and Mostafa Z. Badr -- PPAR-alpha cloning, expression, and characterization / Suong N.T. Ngo and Ross A. McKinnon -- PPARGC1A and PPARG genotyping : beginner's guide to genotyping with unlabeled probes / Viera Habalova and Jozef Židzik -- Generation of an inducible, cardiomyocyte-specific transgenic mouse model with PPAR [beta]/[delta] overexpression / Teayoun Kim [and others] -- Specific knockdown of PPAR[delta] gene in colon cancer cells by lentivirus-mediated RNA Interfering / Lie Yang, Zong-Guang Zhou, and Xiao-Feng Sun -- Dominant-negative and knockdown approaches to studying PPAR activity / Sudhir Singh and Robert G. Bennett -- Producing PPARgamma2 knockdown in mouse liver / Tomomi Yamazaki and Osamu Ezaki -- Adipose tissue-specific PPAR[gamma] gene targeting / Weimin He -- Site-directed mutagenesis to study the role of specific amino acids in the ligand binding domain of PPARs / Nico Mitro [and others] -- PPAR SUMOylation : some useful experimental tips / Benoit Pourcet, Bart Staels, and Corine Glineur -- Analyzing phosphorylation-dependent regulation of subcellular localization and transcriptional activity of transcriptional coactivator NT-PGC-1[alpha] / Ji Suk Chang and Thomas W. Gettys -- In vivo studies of PPAR-chromatin interactions : chromatin immunoprecipitation for single-locus and genomewide analyses / Till Adhikary and Rolf Müller -- FISH analysis using PPAR[gamma]-specific probes for detection of PAX8-PPAR[gamma] translocation in follicular thyroid neoplasms / Noor Akmal Syed Husain Sharifah, Zubaidah Zakaria, and Wai Kit Chia -- Immunohistochemical techniques to identify and localize proteins of interest in paraffin embedded tissue sections / Carolyn M. Komar and Mary Jane Long -- Determination of PPAR expression by western blot / Aziz Guellich and Catherine Coirault -- Fluorescence resonance energy transfer techniques to study ligand-mediated interactions of PPARs with coregulators / Nico Mitro, Cristina Godio, and Maurizio Crestani -- Estimation of the PPAR[alpha] agonism of fibrates by a combined MM-docking approach / Fabio Lannutti, Alessandro Marrone, and Nazzareno Re -- Combined biophysical and cell-based approaches for the assessment of ligand binding to PPAR[gamma] / Silvia Zorrilla and Dolores Pérez-Sala -- Exploring PPAR modulation in experimental mice / Anna Rull [and others] -- Induction of adipogenic differentiation in three-dimensional culture model on a novel microfabricated scaffold / Yoshitaka Miyagawa, Hajime Okita, and Nobutaka Kiyokawa -- Analyzing PPAR[alpha]/ligand interactions by chemical cross-linking and high-resolution mass spectrometry / Mathias Q. Müller, Christian H. Ihling, and Andrea Sinz -- Synthesis, mass spectrometric characterization, and analysis of the PPAR[delta] agonist GW1516 and its major human metabolites : targets in sports drug testing / Mario Thevis [and others] -- LC-MS-based method for the qualitative and quantitative analysis of the novel PPAR[gamma] agonist KR-62980 / Min-Sun Kim and Myung Ae Bae -- Behavioral paradigms to evaluate PPAR modulation in animal models of brain injury / Dana Greene-Schloesser, Caroline I. Schnegg, and Mike E. Robbins.
  • 2013 Springer
    Luis A. del Río, editor.
    Peroxisomes are a class of ubiquitous and dynamic single membrane-bounded cell organelles, devoid of DNA, with an essentially oxidative type of metabolism. In recent years it has become increasingly clear that peroxisomes are involved in a range of important cellular functions in almost all eukaryotic cells. In higher eukaryotes, including humans, peroxisomes catalyze ether phospholipids biosynthesis, fatty acid alpha-oxidation, glyoxylate detoxification, etc, and in humans peroxisomes are associated with several important genetic diseases.
  • 2006 Springer
    edited by Zaki A. Siddiqui.
  • 2005 ScienceDirect
    edited by Helmut Sies, Lester Packer.
    Also available: Print – 2005
  • v. 1- 2=, 2011 Springer
    v. 2, 2011 Springer
    Christian Rommel, Bart Vanhaesebroeck, Peter K. Vogt, editors.
    Vol. 1 / (ix, 306 p.) -- v. 2. / (x, 301 p.).
    Also available: Print – v. 1- 2=, 2011
  • 2012 Springer
    Tamas Balla, Matthias Wymann, John D. York, editors.
    The Phosphatidylinositol 4-Kinases: Don't Call it a Comeback / Shane Minogue and Mark G. Waugh -- PIP Kinases from the Cell Membrane to the Nucleus / Mark Schramp, Andrew Hedman, Weimin Li, Xiaojun Tan and Richard Anderson -- The Phospholipase C Isozymes and Their Regulation / Aurelie Gresset, John Sondek and T. Kendall Harden -- Phosphoinositide 3-Kinases-A Historical Perspective / Alex Toker -- PI3Ks-Drug Targets in Inflammation and Cancer / Matthias Wymann -- Phosphoinositide 3-Kinases in Health and Disease / Alessandra Ghigo, Fulvio Morello, Alessia Perino and Emilio Hirsch -- Phosphoinositide Phosphatases: Just as Important as the Kinases / Jennifer M. Dyson, Clare G. Fedele, Elizabeth M. Davies, Jelena Becanovic and Christina A. Mitchell -- The PTEN and Myotubularin Phosphoinositide 3-Phosphatases: Linking Lipid Signalling to Human Disease / Nicholas R. Leslie, Elizabeth M. Davies, David A. Sheffield, Priyanka Tibarewal and Clare G. Fedele, et al.
  • 2012 Springer
    Tamas Balla, Matthias Wymann, John D. York, editor.
    Phosphoinositides play a major role in cellular signaling and membrane organization. During the last three decades we have learned that enzymes turning over phosphoinositides control vital physiological processes and are involved in the initiation and progression of cancer, inflammation, neurodegenerative, cardiovascular, metabolic disease and more. In two volumes, this book elucidates the crucial mechanisms that control the dynamics of phosphoinositide conversion. Starting out from phosphatidylinositol, a chain of lipid kinases collaborates to generate the oncogenic lipid phosphatidylinositol.
  • 2014 Springer
    Paramjit S. Tappia, Naranjan S. Dhalla, editors.
    Phospholipases generate lipid signaling molecules through their hydrolytic action on phospholipids and are known to regulate function of a variety of cells under normal and diseased conditions. While several physiological, biochemical and molecular techniques have identified key players involved in different disease processes, phospholipases have also emerged as critical players in the pathogenesis of a number of different diseases including cancer and heart disease. In addition, phospholipases are also implicated in such conditions as brain disorder/injury, kidney and immune cell dysfunction. Phospholipases in Health and Disease is a compilation of review articles dedicated to the study of the field with respect to biochemical and molecular mechanisms of normal and abnormal cell function. The wide range of area covered here is of interest to basic research scientists, clinicians and graduate students, who are engaged in studying pathophysiological basis of a variety of diseases. Furthermore, this book highlights the potential of the different phospholipases as therapeutic targets as well as part of prevention strategies. Twenty three articles in this book are organized in four sections that are designed to emphasize the most characterized forms of the phospholipases in mammalian cells. The first section discusses general aspect of phospholipases. Section two covers the role and function of phospholipase A in different pathophysiological conditions. The third section is focussed on phospholipase C which is believed to play a central role in transmembrane signaling. The final section covers phospholipase D which is present in a variety of different cells. The book illustrates that the activation of phospholipases is of fundamental importance in signal transduction affecting cell function. Overall, this book discusses the diverse mechanisms of phospholipase mediated signal transduction in different pathophysiological conditions and raises the possibility of specific forms of phospholipases serving as novel targets for drug development.
  • 2016 Springer Protocols
    edited by Louise von Stechow.
    Thiol-ene-enabled detection of thiophosphorylation as a labeling strategy for phosphoproteins / Kaelyn E. Wilke and Erin E. Carlson -- Phosphopeptide detection with biotin-labeled phos-tag / Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike -- Phosphopeptide enrichment by covalent chromatography after solid phase derivatization of protein digests on reversed phase supports / Heinz Nika, Ruth Hogue Angeletti, and David H. Hawke -- Peptide labeling using isobaric tagging reagents for quantitative phosphoproteomics / Lei Cheng ... [et al.] -- Identification of direct kinase substrates using analogue-sensitive alleles / Daniel A. Rothenberg ... [et al.] -- Quantitative analysis of tissue samples by combining iTRAQ isobaric labeling with selected/multiple reaction monitoring (SRM/MRM) / Ryohei Narumi and Takeshi Tomonaga -- Enrichment strategies in phosphoproteomics / Alexander Leitner -- Phosphopeptide enrichment by immobilized metal affinity chromatography / Tine E. Thingholm and Martin R. Larsen -- Use of titanium dioxide for selective enrichment of phosphorylated peptides / Tine E. Thingholm and Martin R. Larsen -- Sequential elution from IMAC (SIMAC) : an efficient method for enrichment and separation of monoand multi-phosphorylated peptides / Tine E. Thingholm and Martin R. Larsen -- Improving the phosphoproteome coverage for limited sample amounts using TiO2-SIMAC-HILIC (TiSH) phosphopeptide enrichment and fractionation / Kasper Engholm-Keller and Martin R. Larsen -- Offline high pH reversed-phase peptide fractionation for deep phosphoproteome coverage / Tanveer S. Batth and Jesper V. Olsen -- Phosphopeptide enrichment using various magnetic nanocomposites : an overview / Íris L. Batalha and Ana Cecília A. Roque -- Two dimensional gel electrophoresis-based plant phosphoproteomics / Chao Han and Pingfang Yang -- Variable digestion strategies for phosphoproteomics analysis / Humberto Gonczarowska-Jorge ... [et al.] -- Online LC-FAIMS-MS/MS for the analysis of phosphorylation in proteins / Hongyan Zhao, Andrew J. Creese, and Helen J. Cooper -- Simple and reproducible sample preparation for single-shot phosphoproteomics with high sensitivity / Rosa R. Jersie-Christensen, Abida Sultan, and Jesper V. Olsen -- Identification of direct kinase substrates via kinase assay-linked phosphoproteomics / Liang Xue, Justine V. Arrington, and W. Andy Tao -- Phosphoprotein detection by high-throughput flow cytometry / Johannes Landskron and Kjetil Taskén -- Resources for assignment of phosphorylation sites on peptides and proteins / Vaishnavi Ravikumar, Boris Macek, and Ivan Mijakovic -- From phosphosites to kinases / Stephanie Munk ... [et al.] -- Search databases and statistics : pitfalls and best practices in phosphoproteomics / Jan C. Refsgaard, Stephanie Munk, and Lars J. Jensen -- Systems analysis for interpretation of phosphoproteomics data / Stephanie Munk, Jan C. Refsgaard, and Jesper V. Olsen.
  • 2012 Springer
    Dmitri B. Papkovsky...[et al.].
    O2-Sensitive Probes Based on Phosphorescent Metalloporphyrins / Ruslan I. Dmitriev and Dmitri B. Papkovsky -- O2 Analysis on a Fluorescence Spectrometer or Plate Reader / Alexander V. Zhdanov, James Hynes, Ruslan I. Dmitriev and Dmitri B. Papkovsky -- O2 Imaging in Biological Specimens / Andreas Fercher, Alexander V. Zhdanov and Dmitri B. Papkovsky.
  • 2014 Springer Protocols
    edited by Sidney Cambridge.
    Photoinduced damage resulting from fluorescence imaging of live cells / Matthew K. Daddysman, Michael A. Tycon, and Christopher J. Fecko -- Modification of purified proteins with photochemical protection compounds for high-resolution photoactivation of protein function in vitro and in vivo / Sidney Cambridge -- Optochemical activation of kinase function in live cells / Andrei V. Karginov, Klaus M. Hahn, and Alexander Deiters -- Photoswitching of cell surface receptors using tethered ligands / Andreas Reiner and Ehud Y. Isacoff -- Photocontrol of AMPA receptors with a photochromic ligand / Martin Sumser and Philipp Stawski -- Photoconversion of CFP to study neuronal tissue with electron microscopy / Nina Wittenmayer -- Light-inducible gene regulation with engineered zinc finger proteins / Lauren R. Polstein and Charles A. Gersbach -- Manipulation of plasma membrane phosphoinositides using photoinduced protein-protein interactions / Olof Idevall-Hagren and Pietro DeCamilli -- Roadmap to applying optogenetics in neuroscience / Consuelo Fois, Pierre-Hugues Prouvot, and Albrecht Stroh -- Salvaging ruins : reverting blind retinas into functional visual sensors / Marion Mutter, Natalia Swietek, and Thomas A. Münch -- Photoactivated adenylyl cyclases as optogenetic modulators of neuronal activity / Wagner Steuer Costa, Jana Liewald, and Alexander Gottschalk -- Structural basis of photoswitching in fluorescent proteins / Chenxi Duan ... [et al.] -- Using photoactivatable GFP to track axonal transport kinetics / Archan Ganguly and Subhojit Roy -- In vivo cell tracking using PhOTO zebrafish / William P. Dempsey, Hanyu Qin, and Periklis Pantazis -- In vivo optogenetics for light-induced oxidative stress in transgenic zebrafish expressing the killerred photosensitizer protein / Cathleen Teh and Vladimir Korzh -- Photoactivatable fluorescent proteins for super-resolution microscopy / Yuji Ishitsuka, Karin Nienhaus, and G. Ulrich Nienhaus -- pcSOFI as a smart label-based superresolution microscopy technique / Benjamien Moeyaert and Peter Dedecker.
  • 2011 Springer
    Peter R. Bergethon, Kevin Hallock.
    pt. 1. Principles of biophysical inquiry -- pt. 2. Foundations -- pt. 3. Building a model of biomolecular structure -- pt. 4. Function and action biological state space -- pt. 5. Methods for the measuring structure and function -- pt. 6. Physical constants.
  • 2015 Wiley
    Gordon G. Hammes, Sharon Hammes-Schiffer.
    Chapter 1. Heat, Work, and Energy (pages 1-21) -- Chapter 2. Entropy and Gibbs Energy (pages 23-42) -- Chapter 3. Applications of Thermodynamics to Biological Systems (pages 43-76) -- Chapter 4. Thermodynamics Revisited (pages 77-94) -- Chapter 5. Principles of Chemical Kinetics (pages 95-120) -- Chapter 6. Applications of Kinetics to Biological Systems (pages 121-152) -- Chapter 7. Fundamentals of Quantum Mechanics (pages 153-176) -- Chapter 8. Electronic Structure of Atoms and Molecules (pages 177-202) -- Chapter 9. X-ray Crystallography (pages 203-224) -- Chapter 10. Electronic Spectra (pages 225-252) -- Chapter 11. Circular Dichroism, Optical Rotary Dispersion, and Fluorescence Polarization (pages 253-276) -- Chapter 12. Vibrations in Macromolecules (pages 277-288) -- Chapter 13. Principles of Nuclear Magnetic Resonance and Electron Spin Resonance (pages 289-314) -- Chapter 14. Applications of Magnetic Resonance to Biology (pages 315-330) -- Chapter 15. Fundamentals of Statistical Mechanics (pages 331-356) -- Chapter 16. Molecular Simulations (pages 357-382) -- Chapter 17. Ligand Binding to Macromolecules (pages 383-414) -- Chapter 18. Hydrodynamics of Macromolecules (pages 415-440) -- Chapter 19. Mass Spectrometry (pages 441-456).
    Also available: Print – 2015
  • 2007
    Gordon G. Hammes.
    Approachable, yet thorough, this book presents physical chemistry in conceptual terms with a minimum of mathematics. Providing the basic knowledge and tools that every biologist should have to understand the quantitative interpretation of biological phenomena, it covers: Fundamentals of thermodynamics and chemical kinetics ; Fundamentals of spectroscopy and structure determination ; Ligand binding to macromolecules, hydrodynamics, and mass spectrometry. All techniques and concepts are clearly illustrated with relevant applications and examples from the biological sciences. Problems at the end of each chapter reinforce the principles.
  • 2002 CRCnetBASE
    edited by Alejandro G. Marangoni, Suresh S. Narine.
  • 2014 Springer
    Nenad Blau, Marinus Duran, K. Michael Gibson, Carlo Dionisi-Vici, editors.
    Introductory Chapters -- Amino acids -- Organic acids -- Vitamins and neurotransmitter -- Energy metabolism -- Organelles -- Selected disorder -- Biochemical phenotypes of questionable clinical significance -- Profiles.
  • v. 1-2, 1951-54.
    Hrsg. von B. Flaschenträger unter Mitwerkung von E. Lehnartz.
    Status: Not Checked OutLane Catalog Record
    1. Bd. Die Stoffe -- 2. Bd. Der Stoffwechsel.
  • 2012 Springer
    Akhlaq A. Farooqui.
    Effect of Lifestyle, Aging, and Phytochemicals on the Onset of Neurological Disorders -- Beneficial Effects of Extra Virgin Olive Oil (n-9 Fatty Acids) on Neurological Disorders -- Beneficial Effects of Flaxseed Oil (n-3 Fatty Acids) on Neurological Disorders -- Beneficial Effects of Flavonoids on Neurological Disorders -- Beneficial Effects of Green Tea Catechins on Neurological Disorders -- Beneficial Effects of Curcumin on Neurological Disorders -- Beneficial Effects of Resveratrol on Neurological Disorders -- Beneficial Effects of Ginkgo biloba in Neurological Disorders -- Beneficial Effects of Garlic Components on Neurological Disorders -- Beneficial Effects of Propolis on Neurological Disorders -- Perspective and Direction for Future Research on Phytochemicals in Neurological Disorders.
  • 2011 Springer Protocols
    edited by Nico Dissmeyer, Arp Schnittger.
    Guide to the book Plant kinases / Nico Dissmeyer and Arp Schnittger -- The age of protein kinases / Nico Dissmeyer and Arp Schnittger -- Expression and purification of active protein kinases from wheat germ extracts / Boglárka Sonkoly, Viola Bardóczy, and Tamás Mészáros -- Measurement of plant cyclin-dependent kinase activity using immunoprecipitation-coupled and affinity purification-based kinase assays and the baculovirus expression system / Hirofumi Harashima and Masami Sekine -- Mitogen-activated protein kinase activity and reporter gene assays in plants / Róbert Dóczi, Elizabeth Hatzimasoura, and László Bögre -- Use of phospho-site substitutions to analyze the biological relevance of phosphorylation events in regulatory networks / Nico Dissmeyer and Arp Schnittger -- Bacterial assay to study plant sensor histidine kinases / Lukáš Spíchal -- Substrate analysis of Arabidopsis PP2C-type protein phosphatases / Julija Umbrasaite, Alois Schweighofer, and Irute Meskiene -- Modulating and monitoring MAPK activity during programmed cell death in pollen / Shutian Li and Vernonica E. Franklin-Tong -- Sensitizing plant protein kinases to specific inhibition by ATP-competitive molecules / Dor Salomon [and others] -- Fluorescence fluctuation analysis of receptor kinase dimerization / Mark A. Hink, Sacco C. de Vries, and Antonie J.W.G. Visser -- Quantifying degradation rates of transmembrane receptor kinases / Niko Geldner -- Fluorescence correlation spectroscopy and fluorescence recovery after photobleaching to study receptor kinase mobility in planta / Mark Kwaaitaal [and others] -- Bimolecular-fluorescence complementation assay to monitor kinase-substrate interactions in vivo / Stefan Pusch, Nico Dissmeyer, and Arp Schnittger -- Chemical genetic analysis of protein kinase function in plants / Maik Böhmer, Michael Bölker, and Tina Romeis -- Modified metal-oxide affinity enrichment combined with 2D-PAGE and analysis of phosphoproteomes / Thomas Colby [and others] -- Phosphoproteomics using iTRAQ / Alexandra M.E. Jones and Thomas S. Nühse.
  • 2013 Springer Protocols
    edited by Teun Munnick, Ingo Heilmann.
    As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.
  • 2014 Springer Protocols
    edited by George Komis and Jozef Šamaj.
    "Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide established and new MAPK protocols adapted to the challenges posed by working with plants. The book contains 19 chapters which encompass a wide array of methods progressively scaling from the single gene, protein or cell level to large-scale arrays of proteomic, phosphoproteomic and interactomic data in order to uncover previously unidentified plant MAPK signaling pathways and to tackle with the challenging task of substrate identification. Techniques for MAPK sequence analysis and subcellular localization helping to identify their substrates and subcellular compartmentalization are also provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant MAP Kinases: Methods and Protocols represents a collection of useful plant MAPK protocols written by experts in the field for researchers and students."--Back cover.
  • 2015 Springer Protocols
    edited by Waltraud X. Schulze.
    The plant kinome / Monika Zulawski and Waltraud X. Schulze -- Phosphatases in plants / Alois Schweighofer and Irute Meskiene -- Phosphoproteomics in cereals / Pingfang Yang -- Screening of kinase substrates using kinase knockout mutants / Taishi Umezawa -- Phosphopeptide pofiling of receptor kinase mutants / Xu Na Wu and Waltraud X. Schulze -- Combining metabolic N labeling with improved tandem MOAC for enhanced probing of the phosphoproteome / Martin Thomas [and four others] -- Kinase activity and specificity assay using synthetic peptides / Xu Na Wu and Waltraud X. Schulze -- Absolute quantitation of protein posttranslational modification isoform / Zhu Yang and Ning Li -- Phosphorylation stoichiometry determination in plant photosynthetic membranes / Björn Ingelsson, Rikard Fristedt, and Maria V. Turkina -- Phosphopeptide immuno-affinity enrichment to enhance detection of tyrosine phosphorylation in plants / Sharon C. Mithoe and Frank L. H. Menke -- The peptide microarray ChloroPhos1.0 : a screening tool for the identification of Arabidopsis thaliana chloroplast protein kinase substrates / Anna Schönberg and Sacha Baginsky -- Plant protein kinase substrates identification using protein microarrays / Shisong Ma and Savithramma P. Dinesh-Kumar -- Targeted analysis of protein phosphorylation by 2D electrophoresis / Kristin Mayer, Sally Albrecht, and Andreas Schaller -- Computational phosphorylation network reconstruction : methods and resources / Guangyou Duan and Dirk Walther -- Computational identification of protein kinases and kinase-specific substrates in plants / Han Cheng [and three others] -- Databases for plant phosphoproteomics / Waltraud X. Schulze, Qiuming Yao, and Dong Xu -- Phosphorylation site prediction in plants / Qiuming Yao, Waltraud X. Schulze, and Dong Xu.
  • 2011 Springer Protocols
    edited by Alexei V. Tulin.
    Approaches to detect PARP-1 activation in vivo, in situ, and in vitro / Girish M. Shah ... [et al.] -- Quantification of poly(ADP-ribose) in vitro : determination of the ADP-Ribose chain length and branching pattern / Rafael Alvarez-Gonzalez and Myron K. Jacobson -- Assay for protein modification by poly-ADP-Ribose in vitro / Opeyemi A. Olabisi and Chi-Wing Chow -- Identification of distinct amino acids as ADP-Ribose acceptor sites by mass spectrometry / Florian Rosenthal ... [et al.] -- Noncovalent protein interaction with poly(ADP-Ribose) / Maria Malanga and Felix R. Althaus -- Noncovalent pADPr interaction with proteins and competition with RNA for binding to proteins / Yingbiao Ji -- Affinity-based assays for the identification and quantitative evaluation of noncovalent poly(ADP-Ribose)-binding proteins / Jean-Philippe Gagne ... [et al.] -- Detecting and quantifying pADPr in vivo / Yi-Chen Lai ... [et al.] -- Purification of recombinant poly(ADP-Ribose) polymerases / Jean-Christophe Ame ... [et al.] -- Methods for purification of proteins associated with cellular poly(ADP-Ribose) and PARP-specific poly(ADP-Ribose) / Jennifer E. Rood, Anthony K.L. Leung, and Paul Chang -- Purification and characterization of poly(ADP-Ribosyl)ated DNA replication/repair complexes / Cynthia M. Simbulan-Rosenthal, Dean S. Rosenthal, and Mark E. Smulson -- PARP1 genomics : chromatin immunoprecipitation approach using anti-PARP1 antibody (ChIP and ChIP-seq) / Niraj Lodhi and Alexei V. Tulin -- Purification of human PARP-1 and PARP-1 domains from Escherichia coli for structural and biochemical analysis / Marie-France Langelier ... [et al.] -- Regulation of chromatin structure by PARP-1 / Mi Young Kim -- Use of PARP inhibitors in cancer therapy : use as adjuvant with chemotherapy or radiotherapy ; use as a single agent in susceptible patients ; techniques used to identify susceptible patients / Sydney Shall ... [et al.] -- Quantification of PARP activity in human tissues : ex vivo assays in blood cells and immunohistochemistry in human biopsies / Eszter M. Horvath, Zsuzsanna K. Zsengeller, and Csaba Szabo -- Assays for hypermethylation of the BRCA1 gene promoter in tumor cells to predict sensitivity to PARP-inhibitor therapy / Ilsiya Ibragimova and Paul Cairns -- Generation of poly(ADP-ribosyl)ation deficient mutants of the transcription factor, CTCF / Dawn Farrar, Igor Chernukhin, and Elena Klenova -- Phenotypic characterization of Parp-1 and Parp-2 deficient mice and cells / Christian Boehler ... [et al.] -- Poly(ADP-ribosyl)ation pathways in mammals : the advantage of murine PARG null mutation / Yiran Zhou and David W. Koh -- Genetic approaches to targeting multiple PARP genes in a mammalian genome / Mirella L. Meyer-Ficca and Ralph G. Meyer -- Approaches to separate and identify polyADP-Ribosylated proteins using poly(ADP-Ribose) glycohydrolase-knockout Drosophila / Hiroto Nodono ...[et al.] -- Immunolocalization of poly ADP-Ribose on Drosophila polytene chromosomes / Anna Sala and Davide F.V. Corona -- Immunodetection of pADPr in Drosophila tissues : immunostaining of thick sections; immuno-EM / Michal Jarnik -- Poly(ADP-Ribose) metabolism analysis in the Nematode Caenorhabditis elegans / Jean-Francois St-Laurent and Serge Desnoyers -- Genetic dissection of PARylation in the filamentous fungus Neurospora crassa / Gregory O. Kothe -- Purification of the poly-ADP-Ribose polymerase-like thermozyme from the Archaeon Sulfolobus solfataricus / Anna De Maio ... [et al.] -- Strategies employed for the development of PARP inhibitors / Stacie Canan, Karen Maegley, and Nicola Curtin -- Small-molecule collection and high-throughput colorimetric assay to identify PARP1 inhibitors / Elena Kotova, Aaron D. Pinnola, and Alexei V. Tulin.
  • 2006 Springer
    [edited by] Alexander Bürkle.
    Enzymes in poly(ADP-ribose) metabolism / Ralph G. Meyer ... [et al.] -- PARP-2: structure-function relationship / Valørie Schreiber ... [et al.] -- Targeting the poly(ADP-ribose) glycohydrolase (PARG) gene in mammals / Ulrich Cortes and Zhao-Qi Wang -- DNA damage signaling through poly(ADP-ribose) / Maria Malanga and Felix R. Althaus -- Roles of poly(ADP-ribose) metabolism in the regulation of centrosome duplication and in the maintenance of neuronal integrity / Masanao Miwa ... [et al.] -- Functional interactions of PARP-1 with p53 / Rafael Alvarez-Gonzalez ... [et al.] -- Dynamic interaction between PARP-1, PCNA and p21waf1/cip1 / Ennio Prosperi and A. Ivana Scovassi -- Parp-1 as novel coactivator of NF-kB in inflammatory disorders / Paul O. Hassa and Michael O. Hottiger -- PARP and epigenetic regulation / Paola Caiafa -- PARP and the release of apoptosis-inducing factor from mitochondria / Suk Jin Hong, Ted M. Dawson and Valina L. Dawson -- Genome degradation by DNAS1L3 endonuclease: a key PARP-1-regulated event in apoptosis / A. Hamid Boulares, Alexander G. Yakovlev and Mark E. Smulson -- NAD-metabolism and regulatory functions / Mathias Ziegler -- PARP-1 and the shape of cell death / Laszlø Virøg -- Poly(ADP-ribose) polymerase (PARP) and excitotoxicity / Domenico E. Pellegrini-Giampietro, Alberto Chiarugi and Flavio Moroni -- Poly(ADP-ribose) polymerase and ischemia-reperfusion injury / Prabal K. Chatterjee and Christoph Thiemermann -- Role of poly(ADP-ribose) polymerase activation in the pathogenesis of inflammation and circulatory shock / Csaba Szabø -- Role of poly-ADP-ribosylation in cancer development / Mitsuko Masutani ... [et al.] -- PARP inhibitors and cancer therapy / Nicola J. Curtin -- Poly(ADP-ribosyl)ation and aging / Sascha Beneke and Alexander Bürkle.
  • 2010 Springer
    edited by Talila Volk, Karen Artzt.
    STAR trek: an introduction to STAR family proteins and review of quaking (QKI) / Karen Artzt and Jiang I. Wu -- The STAR family member: QKI and cell signaling / Yue Feng and Andrew Bankston -- Insights into the structural basis of RNA : recognition by STAR domain proteins / Sean P. Ryder and Francesca Massi -- Post-translational regulation of STAR proteins and effects on their biological functions / Claudio Sette -- Expression and fuctions of the STAR proteins Sam68 and T-STAR in mammalian spermatogenesis / Ingrid Ehrmann and David J. Elliott -- The role of quaking in mammalian embryonic development / Monica J. Justice and Karen K. Hirschi -- Drosophila STAR proteins: what can be learned from flies? / Talila Volk -- C. elegans STAR proteins, GLD-1 and ASD-2, regulate specific RNA targets to control development / Min-Ho Lee and Tim Schedl -- The branchpoint binding protein: in and out of the spliceosome cycle / Brian C. Rymond -- Reaching for the STARs: linking RNA binding proteins to diseases / Stéphane Richard.
    Also available: Print – 2010
  • 2011 Springer
    Cecilio J. Vidal, editor.
    (Publisher-supplied data) Post-translational modifications serve many different purposes in several cellular processes such as gene expression, protein folding and transport to appropriate cell compartment, protein-lipid and protein-protein interactions, enzyme regulation, signal transduction, cell proliferation and differentiation, protein stability, recycling and degradation. Although several-hundred different modifications are known, the significance of many of them remains unknown. The enormous versatility of the modifications which frequently alter the physico-chemical properties of the respective proteins represents an extraordinary challenge in understanding their physiological role. Since essential cellular functions are regulated by protein modifications, an improvement of current understanding of their meaning might allow new avenues to prevent and/or alleviate human and animal diseases.
  • 2002 Springer Protocols
    edited by Christoph Kannicht.
    Assignment of disulfide bonds in proteins by chemical cleavage and peptide mapping by mass spectrometry / Jiang Wu -- Carbohydrate composition analysis of glycoproteins using highly sensitive fluorescence detection methods / George N. Saddic -- Enzymatical hydrolysis of N-glycans from glycoproteins and fluorescent labeling by 2-aminobenzamide (2-AB) / Rolf Nuck -- Separation of N-glycans by HPLC / Martin Gohlke -- Enzymatic sequence analysis of N-glycans / Christoph Kannicht -- Immunological detection of O-GlcNAc / Monika Rex-Mathes -- Analysis of O-glycosylation / Juan J. Calvete -- Characterization of site-specific glycosylation / Katalin F. Medzihradszky -- Monitoring glycosylation of therapeutic glycoproteins for consistency using highly fluorescent anthranilic acid / Shirish T. Dhume -- Metabolic labelling and structural analysis of glycosylphosphatidylinositols from parasitic protozoa / Peter Gerold -- Analysis of S-acylation of proteins / Michael Veit -- Immunoblotting methods for the study of protein ubiquitination / Edward G. Mimnaugh -- Analysis of methylation and acetylation in E. coli ribosomal proteins / Randy J. Arnold -- Identification of in vivo protein phosphorylation sites with mass spectrometry / Jun Qin -- Analysis of tyrosine-O-sulfation / Jens R. Bundgaard -- a-amidated peptides: approaches for analysis / Gregory P. Mueller -- y-glutamate and B-hydroxyaspartate in proteins / Francis J. Castellino -- Detection of isoAspartate residues as a posttranslational modification of proteins and peptides / Verne Schirch -- Lysine hydroxylation and crosslinking of collagen / Mitsuo Yamauchi -- Heterologous expression in endocrine cells for analysis of posttranslational modifications / Jens R. Bundgaard -- 2D-electrophoresis: detection of glycosylation and influence on spot pattern / Klemens Löster.
  • 2008 Springer Protocols
    Christoph Kannicht, editor.
    Disulfide bond mapping by cyanylation-induced cleavage and mass spectrometry / Jiang Wu -- Detection of post-translational modifications by fluorescent staining of two-dimensional gels / Archana M. Jacob and Chris W. Turck -- Identification of protein phosphorylation sites by advanced LC-ESI-MS/Ms methods / Christoph Weise and Christof Lenz -- Analysis of tyrosine-O-sulfation / Jens R. Bundegaard ... [et al.] -- [alpha]-amidated peptides : approaches for analysis / Gregory P. Mueller and William J. Driscoll -- [gamma]-glutamate and [beta]-hydroxyaspartate in proteins / Francis J. Castellino, Victoria A. Ploplis, and Li Zhang -- Lysine hydroxylation and cross-linking of collagen / Mitsuo Yamauchi and Masashi Shiiba -- Mass spectrometric determination of protein ubiquitination / Carol E. Parker ... [et al.] -- Analysis of sumoylation / Andrea Pichler -- Detection and analysis of protein ISGylation / Tomoharu Takeuchi and Hideyoshi Yokosawa -- Analysis of methylation, acetylation, and other modifications in bacterial ribosomal proteins / Randy J. Arnold, William Running, and James P. Reilly -- Analysis of S-acytlation of proteins / Michael Veit, Evgeni Ponimaskin, and Michael F.G. Schmidt -- Metabolic labeling and structural analysis of glycosylphosphatidylinositols from parasitic protozoa / Nahid Azzouz, Peter Gerol, and Ralph T. Schwarz -- 2-dimensional electrophoresis : detection of glycosylation and influence on spot pattern / Klemens Löster and Christoph Kannicht -- Carbohydrate composition analysis of glycoproteins by HPLC using highly fluorescent anthanilic acid (AA) tag / George N. Saddic, Shirish T. Dhume, and Kalyan R. Anumula -- Enzymatical hydrolysis of N-glycans from glycoproteins and fluorescent labeling by 2-aminobenzamide (2-AB) / Rolf Nuck -- Separation of N-glycans by HPLC / Martin Gohlke and Véronique Blanchard -- Enzymatic sequence analysis of N-glycans by exoglycosidase cleavage and mass spectrometry--detection of Lewis X structures / Christoph Kannicht, Detlef Grunow, and Lothar Lucka -- Immunochemical methods for the rapid screening of the O-glycosidically linked N-acetylglucosamine modification of proteins / Michael Ahrend ... [et al.] -- Analysis of O-glycosylation / Juan J. Calvete and Libia Sanz -- Characterization of site-specific N-glycosylation / Katalin F. Medzihradszky -- Monitoring glycosylation of therapeutic glycoproteins for consistency by HPLC using highly fluorescent anthranilic acid (AA) tag / Shirish T. Dhume, George N. Saddic, and Kalyan R. Anumula -- Comparability and monitoring immunogenic N-linked oligosaccharides from recombinant monoclonal antibodies from two different cell lines using HPLC with fluorescence detection and mass spectrometry / Bruce R.. Kilgore ... [et al.] -- Mass spectrometry and HPLC with fluorescent detection-based orthogonal approaches to characterize N-linked oligosaccharides of recombinant monoclonal antibodies / Adam W. Lucka ... [et al.] -- Web-based computational tools for the prediction and analysis of post-translational modifications of proteins / Vladimir A. Ivanisenko, Dmitry A. Afonnikov, and Nikolay A. Kolchanov.
  • 2008 Springer Protocols
    edited by Jonathan D. Lippiat.
    Identifying transcriptional regulatory regions using reporter genes and DNA-protein interactions by chromatin immunoprecipitation / Lezanne Ooi and Ian C. Wood -- Quantitative RT-PCR methods for investigation of low copy potassium channel gene expresssion in native murine arteries / Alex Cheong, Samuel J. Fountain, and David J. Beech -- Cloning of potassium chanel splice variants from tissues and cells / Lie Chen and Michael J. Shipston -- Chemiluminescence assays to investigate membrane expression and clathrin-mediated endocytosis of KATP channels / Andrew J. Smith and Asipu Sivaprasadarao -- Investigation of KATP channel endocytosis by immunofluorescence / Andrew J. Smith and Asipu Sivaprasadarao -- Investigation of KATP channel endocytosis and cell surface density by biotinylation and western blotting / Andrew J. Smith and Asipu Sivaprasadarao -- Lipid microdomains and K+ channel compartmentation : detergent and non-detergent-based methods for the isolation and characterisation of cholesterol-enriched lipid rafts / Laura J. Sampson and Caroline Dart -- Determination of phosphoinositide binding to K+ channel subunits using a protein-lipid overlay assay / Alison M. Thomas and Andrew Tinker -- Protein complex analysis of native brain potassium channels by proteomics / Guillaume Sandoz and Florian Lesage -- Xenopus oocytes as a heterologous expression system for studying ion channels with the patch-clamp technique / Paolo Tammaro, Kenju Shimomura, and Peter Proks -- Whole-cell recording using the perforated patch clamp technique / Jonathan D. Lippiat -- Recording the activity of ATP-sensitive K+ channels in open-cell cell-attached configuration / Andrei I. Tarasov -- Planar patch clamp : advances in electrophysiology / Andrea Brüggemann ... [et al.] -- Analysing steroid modulation of BKCa channels reconstituted into planar lipid bilayers / Heidi de Wet, Jonathan D. Lippiat, and Marcus Allen -- Using bioluminescence resonance energy transfer to measure ion channel assembly / Gina M. Whitaker and Eric A. Accili -- The use of FRET microscopy to elucidate steady state channel conformational rearrangements and G protein interaction with the GIRK channels / Adi Raveh, Inbal Riven, and Eitan Reuveny -- The voltage-clamp fluorometry technique / Chris S. Gandhi and Riccardo Olcese -- Identification of mutations in the Kir6.2 subunit of the KATP channel / Sarah E. Flanagan and Sian Ellard -- Modulation of potassium ion channel proteins utilising antibodies / Mark L. Dallas, Susan A. Deuchars, and Jim Deuchars -- Fluorescence-based Tl+-influx assays as a novel approach for characterization of small-conductance CA²⁺-activated K+ channel modulators / Susanne Jørgensen, Tina H. Johansen, and Tino Dyhring -- Rubidium efflux as a tool for the pharmacological characterisation of compounds with BK channel opening properties / Neil G. McKay, Robert W. Kirby, and Kim Lawson -- Recording hERG potassium currents and assessing the effects of compounds using the whole-cell patch-clamp technique / Ray M. Helliwell.
  • 2010 CRCnetBASE
    edited by Taosheng Chen.
  • 2006 ScienceDirect
    edited by Michael R. King.
    Traction forces exerted by adherent cells / by Cynthia A. Reinhart-King and Daniel A. Hammer -- Control of endothelial cell adhesion by mechanotransmission from cytoskeleton to substrate / by Rosalind E. Mott and Brian P. Helmke -- Use of hydrodynamic shear stress to analyze cell adhesion / by David Boettiger -- Cellular tensegrity models and cell-substrate interactions / by Dimitrije Stamenović, Ning Wang, and Donald E. Ingber -- Bond formation during cell compression / by Elena Lomakina and Richard E. Waugh -- Dynamics of the neutrophil surface during emigration from blood / Thomas R. Gaborski and James L. McGrath -- Glycocalyx regulation of cell adhesion / Philippe Robert, Laurent Limozin, Anne-Marie Benoliel, Anne Pierres, and Pierre Bongrand -- Atomistic modeling of protein adsorption to ceramic biomaterials in water: a first step toward realistic simulation of the biomaterials surface in vivo / by Alan H. Goldstein -- Model cell membrane surfaces for measuring receptor-ligand interactions / by Craig D. Blanchette, Timothy V. Ratto, and Marjorie L. Longo -- A flow chamber for capillary networks: leukocyte adhesion in capillary sized, ligand-coated micropipettes / by David F.J. Tees, Prithu Sundd, and Douglas J. Goetz -- Adhesion of flowing neutrophils to model vessel surfaces: constraint and regulation by the local hemodynamic environment / Gerard B. Nash and G. Ed Rainger -- Hydrodynamic interactions between cells on reactive surfaces / Dooyoung Lee and Michael R. King -- Dynamics of platelet aggregation and adhesion to reactive surfaces under flow / Nipa A. Mody and Michael R. King.
  • 2012 ClinicalKey
    Gerhard Meisenberg, William H. Simmons.
    Drs. Gerhard Meisenberg and William H. Simmons covers the latest developments in the field, including genome research, the molecular basis of genetic diseases, techniques of DNA sequencing and molecular diagnosis, and more. An updated and expanded collection of figures and access to the fully searchable text, USMLE test questions, clinical case studies and more makes this the resource for understanding all aspects of biochemistry needed in medicine. Tap into the most up-to-date coverage of new developments in genome research, the molecular basis of genetic diseases, techniques of DNA sequencing and molecular diagnosis, RNA interference as a mechanism both for regulation of gene expression and for anti-viral defense, and more. Gain a clear visual understanding through new and updated figures that provide current and relevant guidance. Make the link between basic science and clinical medicine with new Clinical Example boxes in nearly every chapter. Focus on the most essential biochemistry principles and how they apply to clinical medicine.
  • 2007 Springer
    Jan Drenth ; with major contribution from Jeroen Mesters.
  • 2013 CRCnetBASE
    edited by Vincent Béringue, PhD.
    This book contains a selection of chapters aimed to provide a better understanding prion structure and biology. Together these chapters provide an overview of prion biology and underscore some of the challenges we face if we want to understand how this lively pathogen propagates and evolves in mammals. There is also mounting evidence that studying prion biology has a wider relevance due to similarities in the processes of protein misfolding and aggregation between prion disorders and other neurodegenerative disorders such as Alzheimer's, Parkinson's, and Huntington's diseases.
  • 2008 Springer Protocols
    Andrew F. Hill, editor.
    Cell culture models to unravel prion protein function and aberrancies in prion diseases / Katarina Bedecs -- Investigation of PrPc metabolism and function in live cells : methods for studying individual cells and cell populations / Cathryn L. Haigh and David R. Brown -- Immunodetection of PrPsc using western and slot blotting techniques / Hanny Gyllberg and Kajsa Löfgren -- Assaying prions in cell culture : the standard scrapie cell assay (SSCA) and the scrapie cell assay in end point format (SCEPA) / Sukhvir P. Mahal ... [et al.] -- Generation of cell lines propagating infectious prions and the isolation and characterization of cell-derived exosomes / Laura J. Vella and Andrew F. Hill -- Neurotoxicity of prion peptides on cultured cerebellar neurons / Giuseppe D. Ciccotosto, Roberto Cappai, and Anthony R. White -- Understanding the nature of prion diseases using cell-free assays / Victoria A. Lawson -- Methods for conversion of prion protein into amyloid fibrils / Leonid Breydo, Natallia Makarava, and Ilia V. Baskakov -- Amplification of purified prions in vitro / Surachai Supattapone, Nathan R. Deleault, and Judy R. Rees -- Expression and purification of full-length recombinant PrP of high purity / Natallia Makarava and Ilia V. Baskakov -- Analysis of PrP conformation using circular dichroism / Sen Han and Andrew F. Hill -- Effect of copper on the de novo generation of prion protein expressed in Pichia pastoris / Carina Treiber -- Biophysical investigations of the prion protein using electron paramagnetic resonance / Simon C. Drew and Kevin J. Barnham -- Molecular diagnosis of human prion disease / Jonathan D.F. Wadsworth ... [et al.] -- Analysis of endogenous PrPc processing in neuronal and non-neuronal cell lines / Victoria Lewis and Steven J. Collins -- Molecular typing of PrPres in human sporadic CJD brain tissue / Victoria Lewis ... [et al.] -- Transgenic mouse models of prion diseases / Glenn C. Telling -- Quantitiative bioassay of surface-bound prion infectivity / Victoria A. Lawson.
  • 2013 Springer
    Wen-Quan Zou, Pierluigi Gambetti, editors.
    Transmissible spongiform encephalopathies (TSE), now broadly known as prion diseases, have been recognized for nearly 300 years in animals and almost 100 years in humans. However, the nature of the transmissible agent had largely remained a mystery until Stanley Prusiner discovered the infectious isoform of the prion protein (PrP), named prion or scrapie PrP (PrPSc), in 1982. The subsequent modern studies with protein chemistry and molecular biology in cell culture, transgenic animals, and cell-free systems, including the revolutionary protein-misfolding cyclic amplification (PMCA), have greatly advanced our understanding of the pathogenesis of prion diseases and facilitated the identification of new prion diseases in animals and humans. In Prions and Prion Diseases, more than 60 leading researchers and clinicians worldwide provide an up-to-date development in many aspects of these unique infectious pathogens and their associated diseases.Volume II features a variety of animal and human prion diseases, including the newly-identified atypical forms of bovine spongiform encephalopathy and scrapie in animals, and variably protease-sensitive prionopathy in humans, prions in the environment, Tau pathology in human prion disease, transmission of the disease by blood transfusion, mammalian and non-mammalian models, conventional and advanced diagnoses, prion-specific antibodies, as well as decontamination of prions and development of therapeutics of prion diseases, such as the application of immunomodulation. This volume provides up-to-date knowledge about the etiology, pathogenesis, classification, histopathological, and clinical aspects of the highly publicized animal and human prion diseases.
  • 2013 Springer
    edited by Wen-Quan Zou and Pierluigi Gambetti.
    Transmissible spongiform encephalopathies (TSE), now broadly known as prion diseases, have been recognized for nearly 300 years in animals and almost 100 years in humans. However, the nature of the transmissible agent had largely remained a mystery until Stanley Prusiner discovered the infectious isoform of the prion protein (PrP), named prion or scrapie PrP (PrPSc), in 1982. The subsequent modern studies with protein chemistry and molecular biology in cell culture, transgenic animals, and cell-free systems, including the revolutionary protein-misfolding cyclic amplification (PMCA), have greatly advanced our understanding of the pathogenesis of prion diseases and facilitated the identification of new prion diseases in animals and humans. In Prions and Prion Diseases, more than 60 leading researchers and clinicians worldwide provide an up-to-date development in many aspects of these unique infectious pathogens and their associated diseases.Volume I highlights the association of the cellular prion protein (PrPC) with copper and zinc, the potential roles of PrPC in Alzheimer's disease and cancers, insoluble PrPC, PMCA, molecular and cellular mechanisms of PrPSc formation and clearance, possible co-factors involved in the conversion of PrPC into PrPSc, infectious and pathogenic forms of PrP, cell biology of prions, prion strains and their interference, as well as yeast prions and their inheritable and structural traits. This unique volume covers history from the high Middle Ages to the TSE era of Daniel Carleton Gajdusek, followed by the prion era of Stanley Prusiner whose extraordinary discovery opened a new chapter in prion research. Volume I will take you through the fascinating chronicle of prions in mammals, yeast, and fungi.
  • 2014 Springer
    Jaroslava Halper, editor.
    This volume is a reference handbook focusing on diseases like Marfan syndrome, Ehlers-Danlos syndrome, Loeys-Dietz syndrome and other heritable soft connective tissue diseases. The book presents detailed information for both basic scientists and for clinicians seeing patients. It is also a stepping stone for new investigations and studies that goes beyond the facts about the composition and biochemistry of the connective tissue and extracellular matrix, as the authors connect individual components to specific aspects of various soft tissue disorders and to the actual or potential treatment of them. Progress in Heritable Soft Connective Tissue Diseases features very prominent physicians and scientists as contributors who bring their most recent discoveries to the benefit of readers. Their expertise will help clinicians with proper diagnosis of sometimes elusive and uncommon heritable diseases of soft connective tissues. This book also offers an update on the pathophysiology of these diseases, including an emphasis on unifying aspects such as connections between embryonic development of the different types of connective tissues and systems, and the role of TGF-beta in development and physiology of soft tissues. This new set of data explains, at least in part, why many of these disorders are interconnected, though the primary pathophysiological events, such as gene mutations, may be different for each disorder.
    Also available: Print – 2014
  • 2010 Springer
    edited by: A. Graham Pockley, Stuart K. Calderwood and M. Gabriella Santoro.
  • 2013 Springer
    Denis Corbeil, editor.
    Also available: Print – 2013
  • 2011 Springer Protocols
    edited by Majambu Mbikay, Nabil G. Seidah.
    On the Discovery of Precursor Processing -- The Prohormone Theory and the Proprotein Convertases: It Is All About Serendipity -- The Proprotein Convertases, 20 Years Later -- Insights from Bacterial Subtilases into the Mechanisms of Intramolecular Chaperone-Mediated Activation of Furin -- The Novel Role of Cathepsin L for Neuropeptide Production Illustrated by Research Strategies in Chemical Biology with Protease Gene Knockout and Expression -- In Vitro Assay for Protease Activity of Proprotein Convertase Subtilisin Kexins (PCSKs): An Overall Review of Existing and New Methodologies -- Inhibitor Screening of Proprotein Convertases Using Positional Scanning Libraries -- Analyses of PCSK9 Post-Translational Modifications Using Time-of-Flight Mass Spectrometry -- The Molecular Biology of Furin-Like Proprotein Convertases in Vascular Remodelling -- Identification of the Myosin Heavy Polypeptide 9 as a Downstream Effector of the Proprotein Convertases in the Human Colon Carcinoma HT-29 Cells -- Regulation of 7B2 mRNA Translation: Dissecting the Role of Its 5'-Untranslated Region -- Analysis of Epigenetic Alterations to Proprotein Convertase Genes in Disease -- Genetic and Functional Characterization of PCSK1 -- Prohormone Processing in Zebrafish -- Use of Zebrafish and Knockdown Technology to Define Proprotein Convertase Activity -- Characterization of Impaired Processing of Neuropeptides in the Brains of Endoproteases Knockout Mice -- Quantitative Peptidomics of Mice Lacking Peptide-Processing Enzymes -- A Proteomic Protocol to Identify Physiological Substrates of Proprotein Convertases -- Neurophenotyping Genetically-Modified Mice for Social Behavior.
  • 2013 Springer
    Abdulhameed Sabu, Anu Augustine, editors.
    The book entitled "Prospects in Bioscience: Addressing the issues" is a collection of selected research papers presented at the International Conference on Advances in Biological Sciences (ICABS) organized by the Department of Biotechnology and Microbiology and the Inter University Centre for Bioscience, Kannur University, Kerala, India. ICABS witnessed a unique spectrum of Scientific Programmes on the most recent and exciting developments in modern biology. The conference displayed the numerous breakthroughs and significant developments in the important areas of modern biology and their relevance to the welfare of global society. The Book contains 50 well written chapters, each one discussing scientifically organized findings of original research work done in reputed laboratories. Needless to say, they deal with advances in various disciplines of modern biology including Cell and Molecular Biology, Structural Biology, Industrial and Environmental Biotechnology, Food and Agricultural Biotechnology and Medical Biotechnology. As the title rightly indicates, the chapters project the prospects in the respective areas and the issues in them. Specific issues discussed in the book includes development of transgenic plants, bioremediation of toxic industrial effluents, biotransformation for novel antibiotics, biofertilizer development, molecular drug designing and structure elucidation, molecular identification of pathogens, production of anti microbials, biocontrol agents and bioactive molecules, cancer biology, plant breeding and hybrid seed production etc. The book with its contents spreading across the vast arena of modern biology is expected to cater to the need of researchers, technologists and students.
  • 2013 Springer
    Donald J. Tindall, editor.
    Cell biology. Stem cells in the normal and malignant prostate / Norman J. Maitland -- Role of reactive stroma in prostate cancer / Rebeca San Martin, David R. Rowley -- The role of cholesterol in prostate cancer / Keith R. Solomon, Kristine Pelton, Michael R. Freeman -- Genetics. PTEN in prostate cancer / Marcus A. Ruscetti, Hong Wu -- ETS fusion genes in prostate cancer / J. Chad Brenner, Arul M. Chinnaiyan, Scott A. Tomlins -- Cell signaling. Signaling mechanisms of Vav3, a guanine nucleotide exchange factor and androgen receptor coactivator, in physiology and prostate cancer progression / Leah S. Lyons, Kerry L. Burnstein -- Transforming growth factor-beta in prostate cancer / David Danielpour -- The p38 MAPK pathway in prostate cancer / Daniel Djakiew -- NF-kappaB2/p52 in prostate cancer / Nagalakshmi Nadiminty, Ramakumar Tummala, Yezi Zhu -- The functional role of DAB2IP, a homeostatic factor, in prostate cancer / Eun-Jin Yun, Kaijie Wu, Yuh-Shyan Tsai -- Tyrosine kinases in prostate cancer / Yun Qiu, Dhanraj Deshmukh -- Human prostatic acid phosphatase in prostate carcinogenesis / Sakthivel Muniyan, Yu-Wei Chou, Shou-Qiang Ou-Yang -- Hormonal. Truncated androgen receptor splice variants in prostate cancer / Peter E. Lonergan, Donald J. Tindall -- Biology and clinical relevance of estrogen receptors in prostate cancer / Shuk-Mei Ho, Pheruza Tarapore, Ming-Tsung Lee, and Yuet-Kin Leung -- Vitamin D and prostate cancer / Jung-Sun Kim, Justin M. Roberts, Nancy L. Weigel -- HDAC6 regulation of androgen signaling in prostate cancer / Junkui Ai, Zhou Wang -- Beyond the cell cycle : implications of D-type cyclin deregulation in prostate cancer / Michael A. Augello, William F. Ostrander Jr., Karen E. Knudsen -- Cell death. Role of par-4 in prostate cancer / Nidhi Shukla, Nikhil K. Hebbar, Vivek M. Rangnekar -- Autophagy and prostate cancer therapeutics / Hsing-Jien Kung, Chun Changou, Hao G. Nguyen, Joy C. Yang, Christopher P. Evans, Richard J. Bold, and Frank Chuang.
  • v. 3-, 2005- Springer
  • 2013 Springer
    edited by Sajal Chakraborti, Naranjan S. Dhalla.
    In view of rapidly growing research in the deregulation of proteases and their impact in human health and diseases, this book will highlight existing and emerging research in this exciting area. In-depth critical state-of-the-art reviews will be written by established investigators on proteases dysfunctions associated with pathogenesis of different diseases that are known to occur due to deregulation of proteolytic systems. Multidisciplinary approaches demonstrating biochemical and signal transduction mechanisms associated with deregulation of proteases leading to manifestation of the diseases.
  • 2013 Springer Protocols
    edited by Sandra B. Hake, Christian J. Janzen.
    Validation of protein acetylation by mass spectrometry / Barry M. Zee and Benjamin A. Garcia -- Application of the CIRAD mass spectrometry approach for lysine acetylation site discovery / Caroline A. Evans [and others] -- Application of the MIDAS approach for analysis of lysine acetylation sites / Caroline A. Evans [and others] -- Application of high content biology to yield quantitative spatial proteomic information on protein acetylations / Bernard M. Corfe [and others] -- Towards the N-Terminal acetylome : an N-Terminal acetylated peptide enrichment method using CNBr-activated sepharose resin / Xumin Zhang and Peter Højrup -- Identification and analysis of o-acetylated sialoglycoproteins / Chandan Mandal and Chitra Mandal -- HPLC-based quantification of in vitro N-terminal acetylation / Rune H. Evjenth [and others] -- Separation and purification of multiply acetylated proteins using cation-exchange chromatography / Romeo Papazyan and Sean D. Taverna -- In-gel N-acetylation for the quantification of the degree of protein in vivo N-terminal acetylation / Petra Van Damme [and others] -- Computational prediction of lysine acetylation proteome-wide / Amrita Basu -- Generation and characterization of pan-specific anti-acetyllysine antibody / Wei Xu and Shimin Zhao -- Using functional proteome microarrays to study protein lysine acetylation / Jin-ying Lu [and others] -- Quantitation of nucleosome acetylation and other histone posttranslational modifications using microscale NU-ELISA / Bo Dai, Charles Giardina, and Theodore P. Rasmussen -- Preparing semisynthetic and fully synthetic histones H3 and H4 to modify the nucleosome core / John C. Shimko [and others] -- Production of amino-terminally acetylated recombinant proteins in E. coli / Matthew Johnson, Michael A. Geeves, and Daniel P. Mulvihill -- Identification of lysine acetyltransferase substrates using bioorthogonal chemical proteomics / Markus Grammel and Howard C. Hang -- Nonradioactive in vitro assays for histone deacetylases / Alexander-Thomas Hauser, Julia M. Gajer (nee Wagner), and Manfred Jung -- Fluorescence-based acetylation assay using thiol-sensitive probes / Tielong Gao, Chao Yang, and Yujun George Zheng -- Analysis of protein acetyltransferase structure-function relation by surface-enhanced raman scattering (SERS): a tool to screen and characterize small molecule modulators / Mohammed Arif [and others].
  • 2001 ScienceDirect
    edited by K.B. Storey and J.M. Storey.
  • 2014 Springer Protocols
    edited by Richard J. Giannone, Andrew B. Dykstra.
    1D4 : a versatile epitope tag for the purification and characterization of expressed membrane and soluble proteins / Laurie L. Molday and Robert S. Molday -- Affinity purification of heme-tagged proteins / Wesley B. Asher and Kara L. Bren -- Purification of a recombinant protein with cellulose-binding module 3 as the affinity tag / Dongmei Wang and Jiong Hong -- Purification of E. coli proteins using a self-cleaving chitin-binding affinity tag / Michael J. Coolbaugh and David W. Wood -- Simplified protein purification using an autoprocessing, inducible enzyme tag / Aimee Shen -- SUMO as a solubility tag and in vivo cleavage of SUMO fusion proteins with UlP1 / Dennis Kuo, Minghua Nie, and Albert J Courey -- Rescuing aggregation-prone proteins in Escherichia coli with a dual His6-MBP tag / Danielle Needle and David S. Waugh -- Expression, purification, and immobilization of recombinant tamavidin 2 fusion proteins Yashimitsu Takakura, Naomi Oka, and Masako Tsunashima -- Use of tandem affinity chromatography for purification of cannabinoid receptor CB2 / Silvia C. Locatelli-Hoops and Alexei A. Yeliseev -- Detection of protein-protein interactions using tandem affinity purification / Ian Goodfellow and Dalan Bailey -- An improved in vivo biotinylation strategy combined with FLAG and antibody based approaches for affinity purification of protein complexes in mouse embryonic stem cells / Francesco Faiolo [and three others] -- Purification of recombinant proteins with a multifunctional GFP tag / Takashi Murayama and Takuya Kobayashi -- Targeted purification of SnAvi-tagged proteins / Ursula Schäffer, Ralf Baumeister, and Ekkehard Schulze -- An efficient fluorescent protein-based multifunctional affinity purification approach in mammalian cells / Hanhui Ma [and three others] -- Bimolecular affinity purification : a variation of TAP with multiple applications / Petro Starokadomskyy and Ezra Burstein.
  • 2016 Springer Protocols
    edited by David Eliezer.
    Semisynthesis and enzymatic preparation of post-translationally modified α-Synuclein / Bruno Fauvet and Hilal A. Lashuel -- Isotope-labeled amyloids via synthesis, expression, and chemical ligation for use in FTIR, 2D IR, and NMR studies / Tianqi O. Zhang ... [et al.] -- Intermolecular paramagnetic relaxation enhancement (PRE) studies of transient complexes in intrinsically disordered proteins / Maria K. Janowska and Jean Baum -- Detection of helical intermediates during amyloid formation by intrinsically disordered polypeptides and proteins / Andisheh Abedini, Ping Cao, and Daniel P. Raleigh -- Fluorescence correlation spectroscopy : a tool to study protein oligomerization and aggregation in vitro and in vivo / Bankanidhi Sahoo, Kenneth W. Drombosky, and Ronald Wetzel -- Deep UV resonance raman spectroscopy for characterizing amyloid aggregation / Joseph D. Handen and Igor K. Lednev -- Analyzing tau aggregation with electron microscopy / Carol J. Huseby and Jeff Kuret -- Characterization of amyloid oligomers by electrospray ionization-ion mobility spectrometry-mass spectrometry (ESI-IMS-MS) / Charlotte A. Scarff, Alison E. Ashcroft, and Sheena E. Radford -- Formation and characterization of α-Synuclein oligomers / Wojciech Paslawski, Nikolai Lorenzen, and Daniel E. Otzen -- Fluorescence methods for unraveling oligomeric amyloid intermediates / Niels Zijlstra, Nathalie Schilderink, and Vinod Subramaniam -- Preparation of amyloid fibrils for magic-angle spinning solid-state NMR spectroscopy / Marcus D. Tuttle ... [et al.] -- Spin labeling and characterization of tau fibrils using electron paramagnetic resonance (EPR) / Virginia Meyer and Martin Margittai -- Preparation of crystalline samples of amyloid fibrils and oligomers / Asher Moshe, Meytal Landau, and David Eisenberg -- Quenched hydrogen exchange NMR of amyloid fibrils / Andrei T. Alexandrescu -- Studying the early stages of protein aggregation using replica exchange molecular dynamics simulations / Joan- Emma Shea and Zachary A. Levine -- Computational methods for structural and functional studies of Alzheimer's amyloid ion channels / Hyunbum Jang ... [et al.] -- Analyzing ensembles of amyloid proteins using Bayesian statistics / Thomas Gurry ... [et al.] -- In vitro studies of membrane permeability induced by amyloidogenic polypeptides using large unilamellar vesicles / Ping Cao and Daniel P. Raleigh -- Cell models to study cell-to-cell transmission of α-Synuclein / Eun-Jin Bae, He-Jin Lee, and Seung-Jae Lee -- Preparation of amyloid fibrils seeded from brain and meninges / Kathryn P. Scherpel ... [et al.].
  • 2015 Springer Protocols
    edited by Anna S. Kashina.
    Protein arginylation: over 50 years of discovery / Anna S. Kashina -- Recollection of how we came across the protein modification with amino acids by aminoacyl trna-protein transferase / Hideko Kaji and Akira Kaji -- Arginyltransferase: a personal and historical perspective / Ricahrd L. Soffer -- Arginylation in a partially purified fraction of 150k x g supernatants of axoplasm and injured vertebrate nerves / Nicholas A. Ingoglia -- Preparation of ATE1 enzyme from native mammalian tissues / Anna S. Kashina -- Correlated measurement of endogenous ATE1 activity on native acceptor proteins in tissues and cultured cells to detect cellular aging / Hideko Kaji and Akira Kaji -- Assaying the posttranslational arginylation of proteins in cultured cells / Mauricio R. Galiano and Marta E. Hallak -- Assaying ATE1 activity in yeast by [beta]-Gal degradation / Anna S. Kashina -- Bacterial expression and purification of recombinant arginyltransferase (ATE1) and arg-tRNA synthetase (RRS) for arginylation assays / Junling Wang and Anna S. Kashina -- Assaying ATE1 activity in vitro/ Junling Wang and Anna S. Kashina -- High-throughput arginylation assay in microplate format / Sougata Sha, Junling Wang and Anna S. Kashina -- Assay of arginyltransferase activity by a fluorescent HPLC method / Koichi Takao -- Identification of arginylated proteins by mass spectrometry / Anna S. Kashina and Jon R. Yates III -- Analysis of arginylated peptides by subtractive edman degradation / Anna S. Kashina and Jon R. Yates III -- Transferase-mediated labeling of protein N-termini with click chemistry handles / Anne M. Wagner [and four others] -- Applying arginylation for bottom-up proteomics / H. Alexander Ebhardt -- Development of new tools for the studies of protein arginylation / Anna S. Kashina.
  • 2015 Springer Protocols
    edited by Brendan P. Orner.
    Encapsulation of nanoparticles in virus protein shells / Irina B. Tsvetkova and Bogdan G. Dragnea -- Use of protein cages as a template for confined synthesis of inorganic and organic nanoparticles / Masaki Uchida [and three others] -- Ferritin encapsulation and templated synthesis of inorganic nanoparticles / Katherine W. Pulsipher and Ivan J. Dmochowski -- Determining the relaxivity values of protein cage-templated nanoparticles using magnetic resonance imaging / Barindra Sana and Sierin Lim -- Computationally assisted engineering of protein cages / Maziar S. Andrejani and Brendan P. Orner -- Recombinant expression and purification of "virus-like" bacterial encapsulin protein cages / W. Frederik Rurup, Jeroen J.L.M. Cornelissen, and Melisa S.T. Koay -- Production of bacterial microcompartments / Jonathan K. Lassila -- Detection of protein cage assembly with bisarsenic fluorescent probes / Thomas A. Cornell and Brendan P. Orner -- Determining the role of metal binding in protein cage assembly / Anne Grove, Ambuj K. Kushwaha, and Khoa H. Nguyen -- Differential scanning calorimetry to quantify the stability of protein cages / Yu Zhang and Maziar S. Ardejani -- Material properties of viral nanocages explored by atomic force microscopy / Mariska G.M. van Rosmalen, Wouter H. Roos, and Gijs J.L. Wuite -- Computational mechanics of viral capsids / Melissa G. Gibbons, Luigi E. Perotti, and William S. Klug.
  • 2014 Springer
    Ke-li Han, Xin Zhang, Ming-jun Yang, editors.
    "This book discusses how biological molecules exert their function and regulate biological processes, with a clear focus on how conformational dynamics of proteins are critical in this respect. In the last decade, the advancements in computational biology, nuclear magnetic resonance including paramagnetic relaxation enhancement, and fluorescence-based ensemble/single-molecule techniques have shown that biological molecules (proteins, DNAs and RNAs) fluctuate under equilibrium conditions. The conformational and energetic spaces that these fluctuations explore likely contain active conformations that are critical for their function. More interestingly, these fluctuations can respond actively to external cues, which introduces layers of tight regulation on the biological processes that they dictate. A growing number of studies have suggested that conformational dynamics of proteins govern their role in regulating biological functions, examples of this regulation can be found in signal transduction, molecular recognition, apoptosis, protein / ion / other molecules translocation and gene expression"--Publisher's description.
    Also available: Print – 2014
  • 2014 Springer
    Anthony P. Nicholas, Sanjoy K. Bhattacharya, editors.
    Physiological pathways of PAD activity and citrullinated epitope generation -- from citrullination to specific immunity and disease in rheumatoid arthritis -- The role of citrullinated proteins in the pathophysiology of rheumatoid arthritis -- Protein citrullination: the link between rheumatoid arthritis and periodontitis? -- From genes and environment to anti-culture immunity in rheumatoid arthritis: The role of the lungs -- Neutrophils and their contribution to autoimmunity in rheumatoid arthritis -- Deimination in skin and regulation of PAD expression in keratinocytes -- Importance of citrullination on hair protein molecular assembly during trichocytic differentiation -- Deimination in the peripheral nervous system: A wallflower existence -- Deimination in multiple sclerosis and experimental autoimmune encephalomyelitis -- Protein hypercitrullination in CNS demyelinating disease reversed by PAD inhibition -- Deimination in prion diseases -- Deimination in Alzheimer's disease -- Ongoing studies of deimination in neurodegenerative diseases using the F95 antibody -- The role of protein deimination in epigenetics -- Identifying citrullination sites by mass spectroscopy -- Homocitrulline\U+2014\an analogue and confounder related to citrulline -- Picking the PAD lock: Chemical and biological approaches to identify PAD substrates and inhibitors.
  • 2014 Springer Protocols
    edited by Valentin Köhler.
    De novo design of stable [alpha]-helices / Alexander Yakimov, Georgy Rychkov, and Michael Petukhov -- Design of monomeric water-soluble [Beta]-hairpin and [Beta]-sheet peptides/ M. Angeles Jiménez -- Combination of theoretical and experimental approaches for the design and study of fibril-forming peptides / Phanourios Tamamis ... [et al.] -- Posttranslational incorporation of noncanonical amino acids in the RNase S system by semisynthetic protein assembly / Maika Genz and Norbert Sträter -- Design, synthesis, and study of fluorinated proteins / Benjamin C. Buer and E. Neil G. Marsh -- High-quality combinatorial protein libraries using the binary patterning approach / Luke H. Bradley -- Methods for library-scale computational protein design / Lucas B. Johnson, Thaddaus R. Huber, and Christopher D. Snow -- Symmetric protein architecture in protein design : top-down symmetric deconstruction / Liam M. Longo and Michael Blaber -- Identification of protein scaffolds for enzyme design using scaffold selection / André C. Stiel, Kaspar Feldmeier, and Birte Höcker -- Computational design of novel enzymes without cofactors / Matthew D. Smith, Alexandre Zanghellini, and Daniela Grabs-Röthlisberger -- De novo design of peptide scaffolds as novel preorganized ligands for metal-ion coordination / Aimee J. Gamble and Anna F. A. Peacock -- Computational design of metalloproteins / Avanish S. Parmar, Douglas Pike, and Vikas Nanda -- Incorporation of modified and artificial cofactors into naturally occurring protein scaffolds / Koji Oohora and Takashi Hayashi -- Computational redesign of metalloenzymes for catalyzing new reactions / Per Jr. Greisen and Sagar D. Khare.
  • 2006 Springer Protocols
    edited by Raphael Guerois, Manuela López de la Paz.
    Design of structural elementary motifs. Structure and stability of the [alpha]-helix: lessons for design / Neil Errington, Teuku Iqbalsyah, and Andrew J. Doig. De novo design of monomeric [beta]-hairpin and [beta]-sheet peptides / David Pantoja-Uceda, Clara M. Santiveri, and M. Angeles Jiménez. De novo proteins from binary-patterned combinatorial libraries / Luke H. Bradley, Peter P. Thumfort, and Michael H. Hecht. Non-protein amino acids in the design of secondary structure scaffolds / Radhakrishnan Mahalakshmi and Padmanabhan Balaram. Design and synthesis of [beta]-peptides with biological activity / Marc J. Koyack and Richard P. Cheng -- Design of entire proteins and protein complexes. Design of miniproteins by the transfer of active sites onto small-size scaffolds / Franc̦ois Stricher, Loïc Martin, and Claudio Vita. Consensus design as a tool for engineering repeat proteins / Tommi Kajander, Aitziber L. Cortajarena, and Lynne Regan. Multiple sequence alignment as a guideline for protein engineering strategies / Alan R. Davidson. Sequence search methods and scoring functions for the design of protein structures / Hocine Madaoui, Emmanuelle Becker, and Raphael Guerois. Prediction of protein-protein interaction based on structure / Gregorio Fernandez-Ballester and Luis Serrano. Electrostatic design of protein-protein association rates / Gideon Schreiber, Yossi Shaul, and Kay E. Gottschalk -- Design of amyloidogenic polypeptides and amyloid inhibitors. Peptide model systems for amyloid fiber formation: design strategies and validation methods / Alexandra Esteras-Chopo, María Teresa Pastor, and Manuela López de la Paz. Protein misfolding disorders and rational design of antimisfolding agents / Lisbell D. Estrada, June Yowtak, and Claudio Soto.
  • 2014 Springer Protocols
    edited by Nikolaos E. Labrou.
    Protein Purification: An Overview -- Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE -- High Throughput Process Development: I. Process Chromatography -- High Throughput Process Development: II. Membrane Chromatography -- Media Selection in Ion Exchange Chromatography in a Single Microplate -- High Throughput Screening of Dye-Ligands for Chromatography -- Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips -- Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline -- Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants -- Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins -- Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP) -- Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm -- Immunoglobulin Purification by Caprylic Acid -- Affinity Tags in Protein Purification and Peptide Enrichment: An Overview -- Expression and Purification of Large Active GST Fusion Enzymes -- Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins -- Zbasic -- A Purification Tag for Selective Ion-Exchange Recovery -- An Orthogonal Fusion Tag for Efficient Protein Purification -- Phage Display of Engineered Binding Proteins -- Biomimetic Affinity Ligands for Protein Purification -- Synthesis and Application of Dye-Ligand Affinity Adsorbents -- Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification -- Affinity Chromatography of Proteins on Monolithic Columns -- Sample Displacement Batch Chromatography of Proteins -- Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches -- Preparation of Monolithic Affinity Media for Nano-Liquid Chromatography Applications -- Proteomic Analysis of Complex Protein Samples by MALDI TOF Mass Spectrometry -- Modern Bioanalysis of Proteins by Electrophoretic Techniques -- Protein Structure Validation and Analysis with X-Ray Crystallography -- Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes -- Stabilization of Therapeutic Proteins in Aqueous Solutions and Freeze-Dried Solids: An Overview -- Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin -- G Protein-Coupled Receptor Expression and Purification.- (Hyper)thermophilic Enzymes: Production and Purification -- Affinity Chromatography for Antibody Purification -- Screening and Purification of Recombinant Lignocellulolytic Enzymes -- Purification of PEGylated Proteins, with the Example of PEGylated lysozyme and PEGylated scFv -- One-Step Purification of Glutamate decarboxylase from E. coli Using Aqueous Two Phase System.
  • 2014 Springer Protocols
    edited by Dennis R. Livesay.
    Monitoring Side-Chain Dynamics of Proteins Using 2H Relaxation -- CPMG Relaxation Dispersion -- Confocal Single-Molecule FRET for Protein Conformational Dynamics -- Protein Structural Dynamics Revealed by Site-directed Spin Labeling and Multifrequency EPR -- Probing Backbone Dynamics With Hydrogen/Deuterium Exchange Mass Spectrometry -- Carbon-Deuterium Bonds as Non-perturbative Infrared Probes of Protein Dynamics, Electrostatics, Heterogeneity, and Folding -- Balancing Bond, Nonbond and G?-like Terms in Coarse Grain Simulations of Conformational Dynamics -- Tutorial on Building Markov State Models with MSMBuilder and Coarse-graining them with BACE -- Analysis of Protein Conformational Transitions Using Elastic Network Model -- Geometric Simulation of Flexible Motion in Proteins -- Principal Component Analysis: A Method for Determining the Essential Dynamics of Proteins -- A Case Study Comparing Quantitative Stability/Flexibility Relationships Across Five Metallo-?-Lactamases Highlighting Differences within NDM-1 -- Towards Comprehensive Analysis of Protein Family Quantitative Stability/Flexibility Relationships using Homology Models -- Using the COREX/BEST Server to Model the Native State Ensemble -- Morphing Methods to Visualize Coarse-grained Protein Dynamics.
  • 2012 Springer Protocols
    edited by Biji T. Kurien and R. Hal Scofield.
    How it all began : a personal history of gel electrophoresis / Oliver Smithies -- Introduction to protein electrophoresis / Pothur R. Srinivas -- Protein quantification methods to determine protein concentration prior to electrophoresis / J.P. Dean Goldring -- Protein solubilization : attend to the choice of lysis buffer / Mandy Peach, Noelle Marsh, and Daniel J. MacPhee -- Native polyacrylamide gels / Claudia Arndt [and others] -- Cationic electrophoresis / Engelbert Buxbaum -- SARCOSYL-PAGE : a new electrophoretic method for the separation and immunological detection of PEGylated proteins / Christian Reichel -- Tricine-SDS-PAGE / Syed R. Haider, Helen J. Reid, and Barry L. Sharp -- Analysis of protein glycation using phenylboronate acrylamide gel electrophoresis / Marta P. Pereira Morais [and others] -- Protein electrophoresis in agarose gels for separating high molecular weight proteins / Marion L. Greaser and Chad M. Warren -- Increase in local protein concentration by field-inversion gel electrophoresis / Henghang Tsai and Hon-chiu Eastwood Leung -- Combined free flow electrophoresis and DIGE approach to compare proteins in complex biological samples / Kim Y.C. Fung [and others] -- Immunofixation electrophoresis for identification of proteins and specific antibodies / Gyorgy Csako -- Microchip electrophoresis, with respect to "Profiling of Abeta Peptides in the Cerebrospinal Fluid of Patients with Alzheimer's Disease" / Mohamad Reza Mohamadi [and others] -- Foodomics approach : CE-MS for comparative metabolomics of colon cancer cells treated with dietary polyphenols / Mustafa Celebier [and others] -- Peptide fractionation by SDS-free polyacrylamide gel electrophoresis for proteomic analysis via DF-PAGE / Yassel Ramos, Vladimir Besada, and Lila Castellanos-Serra -- Tris-acetate polyacrylamide gradient gels for the simultaneous electrophoretic analysis of proteins of very high and low molecular Mass / Monica Cubillos-Rojas [and others] -- SDS-PAGE for ³⁵S immunoprecipitation and immunoprecipitation western blotting / Edward P. Trieu and Ira N. Targoff -- Two-dimensional gel electrophoresis : vertical isoelectric focusing / Yaser Dorri -- Isoelectric focusing in agarose gel for detection of oligoclonal bands in cerebrospinal and other biological fluids / Gyorgy Csako -- Native flatbed isoelectric focusing for determining antibody clonotype distribution / Biji T. Kurien and R. Hal Scofield -- Two-dimensional gel electrophoresis : glass tube-based IEF followed by SDS-PAGE / Hiroyuki Matsumoto [and others] -- One-dimensional and two-dimensional immobilized metal affinity electrophoresis / Bao-Shiang Lee [and others] -- Two-dimensional difference gel electrophoresis / Jonathan S. Minden -- Protein identification on archived 2-D dels / Hiroyuki Matsumoto, Nobuaki Takemori, and Naoka Komori -- Diagonal Electrophoresis for the Detection of Protein Disulfides / Brian McDonagh -- Two-dimensional blue native/SDS gel electrophoresis of multiprotein complexes / Jean Paul Lasserre and Armelle Menard -- Immunoelectrophoresis : a method with many faces / Gyorgy Csako -- Cell surface protein biotinylation for SDS-PAGE analysis / Giuliano Elia -- Multichannel gel electrophoresis and continuous fraction collection apparatus for high-throughput protein separation and characterization / Ming Dong [and others] -- Gel-absorption-based sample preparation method for shotgun analysis of membrane proteome / Xianchun Wang and Songping Liang -- Interactions of hemoglobin in live red blood cells measured by the electrophoresis release test / Yan Su, Lijun Gao, and Wenbin Qin -- Extraction of proteins from gels : a brief review / Biji T. Kurien and R. Hal Scofield -- New algorithm for the simulation of SDS 2D-PAGE datasets / Emilio Marengo and Elisa Robotti -- Isolation of proteins from polyacrylamide gels / Irene Michalk [and others] -- Gel drying methods / Slava Stamova [and others] -- Long-term, buffer-less, wet gel storage in non-sealed polyethylene bags / Biji T. Kurien and R. Hal Scofield -- Brief review of other notable electrophoretic methods / James D. Fesmire -- Protein stains and applications / Ranjini K. Sundaram, Natarajan Balasubramaniyan -- Coomassie-brilliant blue staining of polyacrylamide gels / Claudia Arndt [and others] -- Accelerated coomassie blue staining and destaining of SDS-PAGE gels with application of heat / Biji T. Kurien and R. Hal Scofield -- Silver staining techniques of polyacrylamide gels / Holger Bartsch [and others] -- Revisit of imidazole-zinc reverse stain for protein polyacrylamide gel electrophoresis / Han-Min Chen -- Counterion dye staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tryptic gel digestion of stained protein for mass spectrometry / Wei-Tao Cong [and others] -- TEMED-enhanced photoluminescent imaging of human serum proteins by quantum dots after PAGE / Na Na and Jin Ouyang -- Microwave-assisted protein staining, destaining, and in-gel/in-solution digestion of proteins / Jennie R. Lill and Victor J. Nesatyy -- Electrophoretic separation and detection of metalloproteins by x-ray fluorescence mapping / Tripti Khare, Yasmin Chishti, and Lydia A. Finney -- Fluorescent staining of gels / Engelbert Buxbaum -- Single-step simultaneous protein staining procedure for polyacrylamide gels and nitrocellulose membranes by alta during western blot analysis / Jayanta K. Pal, Sunil K. Berwal, and Rupali N. Soni -- Detection of glycoproteins in polyacrylamide gels using Pro-Q Emerald 300 dye, a fluorescent periodate schiff-base stain / Padmaja Mehta-D'souza -- Spicy SDS-PAGE gels : curcumin/turmeric as an environment-friendly protein stain / Biji T. Kurien, Yaser Dorri, and R. Hal Scofield -- In-gel staining of proteins in native polyacrylamide gel electrophoresis using meso-tetrakis(4-sulfonatophenyl) porphyrin / K. Divakar, G. Nandhini Devi, and Pennathur Gautam -- Rapid detection of proteins in polyacrylamide electrophoresis gels with direct red 81 and amido black / David Choveaux, Robert G.E. Krause, and J.P. Dean Goldring -- Zymographic assay of oxidases using peroxidase or hemin entrapped in polyacrylamide gel / Mircea Alexandru Mateescu [and others] -- Coomassie brilliant blue removal/disposal from gel destain and used gel stain in an environment-friendly manner / Biji T. Kurien and Yaser Dorri -- Brief review of other notable protein detection methods on acrylamide gels / Biji T. Kurien and R. Hal Scofield -- Ultrasensitive protein detection and imaging : comparison of lumitein, proteoSilver, SYPRO® Ruby, and Coomassie® Brilliant Blue Gel stains / Guang Ren, Carl K. Okerberg, and Suresh T. Mathews -- Common artifacts and mistakes made in electrophoresis / Biji T. Kurien and R. Hal Scofield.
  • pt. A-B, 2012. ScienceDirect
    pt. B ScienceDirect
    edited by K. Dane Wittrup, Gregory L. Verdine.
  • 2012 Springer Protocols
    edited by James L. Hartley.
    Why proteins in mammalian cells? / James L. Hartley -- Large-scale transfection of mammalian cells / Lucia Baldi [and others] -- Selection of high expressing mammalian cells by surface display of reporters / Christine T. DeMaria -- Expression of secreted protein in mammalian cells using baculovirus particles / Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash -- Transfection of difficult-to-transfect primary mammalian cells / Oliver Gresch and Ludger Altrogge -- Stable protein expression in mammalian cells using baculoviruses / Andreas Lackner [and others] -- Using matrix attachment regions to improve recombinant protein production / Niamh Harraghy [and others] -- Controlling apoptosis to optimize yields of prtoeins from mammalian cells / Matthew P. Zustiak [and others] -- Post-transcriptional regulatory elements for enhancing transient gene expression levels in mammalian cells / Mariati [and others] -- Converting monoclonal antibodies into Fab fragments for transient expression in mammalian cells / Joanne E. Nettleship [and others] -- Generation of high-expressing cells by methotrexate amplification of destabilized dihydrofolate reductase selection marker / Say Kong Ng -- Tools for coproducing multiple proteins in mammalian cells / Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia -- Identification and characterization of protein glycosylation using specific endo- and exoglycosidases / Paula Magnelli, Alicia Bielik, and Ellen Guthrie -- Strategies for efficient transfection of CHO-cells with plasmid DNA / Renate Kunert and Karola Vorauer-Uhl -- Methods for constructing clones for protein expression in mammalian cells / Takefumi Sone and Fumio Imamoto -- Optimizing transient recombinant protein expression in mammalian cells / Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito.
  • 2006 Springer
    Bengt Nölting.
    Introduction -- Structure of proteins -- Physical interactions that determine the properties of proteins -- Calculation of the kinetic rate constants -- High kinetic resolution of protein folding events -- Kinetic methods for slow reactions -- Resolution of protein structures in solution -- High structural resolution of transient protein conformations -- Experimental problems of the kinetic and structural resolution of reactions that involve proteins -- The folding pathway of protein (barstar) at the resolution of individual residues from microseconds to seconds -- Highly resolved folding pathways and mechanisms of six proteins -- Structural determinants of the rate of protein folding -- Evolutionary computer programming of protein structure and folding.
  • 2011 Springer Protocols
    edited by Andrew F. Hill, Kevin J. Barnham, Stephen P. Bottomley, Roberto Cappai.
    Strategies for boosting the accumulation of correctly folded recombinant proteins expressed in Escherichia coli / Ario de Marco -- Escherichia coli cell-free system for recombinant protein synthesis on a milligram scale / Luke A. Miles [and others] -- Synthesis of peptide sequences derived from fibril-forming proteins / Denis B. Scanlon and John A. Karas -- Refolding your protein with a little help from REFOLD / Jennifer Phan [and others] -- Circular dichroism and its use in protein-folding studies / David T. Clarke -- Distance measurements by continuous wave EPR spectroscopy to monitor protein folding / James A. Cooke and Louise J. Brown -- Solution-state nuclear magnetic resonance spectroscopy and protein folding / Lisa D. Cabrita [and others] -- Diagnostics for amyloid fibril formation : where to begin / Danny M. Hatters and Michael D.W. Griffin -- Probing protein aggregation with quartz crystal microbalances / Tuomas P.J. Knowles [and others] -- Dried and hydrated x-ray scattering analysis of amyloid fibrils / Sally L. Gras and Adam M. Squires -- Solid-state NMR of amyloid membrane interactions / John D. Gehman and Frances Separovic -- Sedimentation velocity analysis of amyloid fibrils / Chi Le Lan Pham, Yee-Foong Mok, and Geoffrey J. Howlett -- Transmission electron microscopy of amyloid fibrils / Sally L. Gras, Lynne J. Waddington, and Kenneth N. Goldie -- Surface plasmon resonance spectroscopy : a new lead in studying the membrane binding of amyloidogenic transthyretin / Xu Hou, David H. Small, and Marie-Isabel Aguilar -- Elucidating the role of metals in Alzheimer's disease through the use of surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry / Andrew D. Watt, Keyla A. Perez, and Lin Wai Hung.
  • 2006 Springer Protocols
    edited by Yawen Bai and Ruth Nussinov.
  • 2007 Springer
    edited by Peter Schuck.

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