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Biochemistry

  • 2010 Springer
    Quinn, Peter J.; Wang, Xiaoyuan.
    Endotoxins: Lipopolysaccharides of Gram-negative bacteria / X. Wang and P.J. Quinn -- Purification and characterization of lipopolysaccharides / X. Wang ... [et al.] -- Endotoxins: Relationship between structure, function, and activity / K. Brandenburg, A.B. Schromm and T. Gutsmann -- The diversity of the core oligosaccharide in lipopolysaccharides / A. Silipo and A. Molinaro -- Salmonella-regulated lipopolysaccharide modifications / S.M. Richards, K.L. Strandberg and J.S. Gunn -- The variation of O antigens in Gram-negative bacteria / L. Wang, Q. Wang and P.R. Reeves -- Regulators of TLR4 signaling by endotoxins / A.F. McGettrick and L.A.J. O'Neill -- Membrane partitioning: Is location everything when it comes to endotoxin recognition? / M. Triantafilou and K. Triantafilou -- Endotoxin detection - from limulus amebocyte lysate to recombinant Factor C / J.L. Ding and B. Ho -- The role of endotoxin in infection: Helicobacter pylori and Campylobacter jejuni / A.P. Moran -- The role of pseudomonas lipopolysaccharide in cystic fibrosis airway infection / S.M. Moskowitz and R.K. Ernst -- Development of small-molecule endotoxin sequestering agents / S.A. David and D. Sil -- Development of an anti-endotoxin vaccine for sepsis / A.S. Cross -- Synthetic and natural TLR4 agonists as safe and effective vaccine adjuvants / C.B. Fox ... [et al.] -- Targeting endotoxin in the treatment of sepsis / J.-S. Rachoin, C.A. Schorr and R.P. Dellinger -- Lipopolysaccharides in rhizobium-legume symbioses / R.W. Carlson, L.S. Forsberg and E.L. Kannenberg -- Lipopolysaccharides and plant innate immunity / G. Erbs ... [et al.].
  • 2010 Springer Protocols
    Mackay, Joel P.; Segal, David J.
    The generation of zinc finger proteins by modular assembly / Mital S. Bhakta and David J. Segal -- Engineering single Cys2His2 zinc finger domains using a bacterial cell-based two-hybrid selection system / Stacey Thibodeau-Beganny, Morgan L. Maeder, and J. Keith Joung -- Bipartite selection of zinc fingers by phage display for any 9-bp DNA target site / Jia-Ching Shieh -- Structure-based DNA-binding prediction and design / Andreu Alibés, Luis Serrano, and Alejandro D. Nadra -- Generation of cell-permeable artificial zinc finger protein variants / Takashi Sera -- Inhibition of viral transcription using designed zinc finger proteins / Kimberley A. Hoeksema and D. Lorne J. Tyrrell -- Modulation of gene expression using zinc finger-based artificial transcription factors / Sabine Stolzenburg ... [et al.] -- Construction of combinatorial libraries that encode zinc finger-based transcription factors / Seokjoong Kim, Eun Ji Kim, and Jin-Soo Kim -- Silencing of gene expression by targeted DNA methylation : concepts and approaches / Renata Z. Jurkowska and Albert Jeltsch -- Remodeling genomes with artificial transcription factors (ATFs) / Adriana S. Beltran and Pilar Blancafort -- Transgenic mice expressing an artificial zinc finger regulator targeting an endogenous gene / Claudio Passananti ... [et al.] -- Artificial zinc finger nucleases for DNA cloning / Vardit Zeevi, Andriy Tovkach, and Tzvi Tzfira -- In vitro assessment of zinc finger nuclease activity / Toni Cathomen and Cem Șöllü -- Quantification of zinc finger nuclease-associated toxicity / Tatjana I. Cornu and Toni Cathomen -- A rapid and general assay for monitoring endogenous gene modification / Dmitry Y. Guschin ... [et al.] -- Engineered zinc finger proteins for manipulation of the human mitochondrial genome / Michal Minczuk -- High-efficiency gene targeting in Drosophila with zinc finger nucleases / Dana Carroll, Kelly J. Beumer, and Jonathan K. Trautman -- Using zinc finger nucleases for efficient and heritable gene disruption in zebrafish / Jasmine M. McCammon and Sharon L. Amacher -- A transient assay for monitoring zinc finger nuclease activity at endogenous plant gene targets / Justin P. Hoshaw ... [et al.] -- Validation and expression of zinc finger nucleases in plant cells / Andriy Tovkach, Vardit Zeevi, and Tzvi Tzfira -- Non-FokI-based zinc finger nucleases / Miki Imanishi, Shigeru Negi, and Yukio Sugiura -- Designing and testing chimeric zinc finger transposases / Matthew H. Wilson and Alfred L. George -- Seeing genetic and epigenetic information without DNA denaturation using sequence-enabled reassembly (SEER) / Jason R. Porter ... [et al.] -- Zinc finger-mediated live cell imaging in Arabidopsis roots / Beatrice I. Lindhout, Tobias Meckel, and Bert J. van der Zaal -- Biophysical analysis of the interaction of toxic metal ions and oxidants with the zinc finger domain of XPA / Andrea Hartwig, Tanja Schwerdtle, and Wojciech Bal -- Preparation and zinc-binding properties of multi-fingered zinc-sensing domains / John H. Laity and Linda S. Feng -- Using ChIP-seq technology to identify targets of zinc finger transcription factors / Henriette O'Geen, Seth Frietze, and Peggy J. Farnham -- Crystallization of zinc finger proteins bound to DNA / Nancy C. Horton and Chad K. Park -- Beyond DNA : zinc finger domains as RNA-binding modules / Josep Font and Joel P. Mackay.
  • 2013 Springer
    Divakar, Soundar.
    Transformations using enzymes have been extensively investigated in the last two decades and the results promise great potential for this growing field, especially in the area of synthetic organic chemistry mainly due to of its many advantages. Accordingly, this book has attempted to bring out the advantages of using enzymes involving complex underivatized and unprotected substrates in non-polar media under homogenous and heterogeneous reaction conditions. Merits and demerits of using enzymes in terms of yields and selectivity/specificity are presented without any prejudice. Almost all the reactions dealt with are from the author's laboratory comprising diverse substrates, and the catalysis involves two important hydrolyzing enzymes, extensively examined for the reverse reactions. Thus, esterification involving lipses and glycosylation involving glycosidases were investigated with respect to various strategies like optimization of reaction conditions, response surface methodology and kinetics, carrying out reactions under solvent, non-solvent and super critical carbon dioxide conditions. In short, the work presented is to ensure the comprehension of the problems faced by the researchers in this area so as to work out further efficient strategies for carrying out enzymatic transformations in the laboratory successfully with better yields and specificity.
  • 2013 Springer Protocols
    Samuelson, James C.
    1. Tripartite fusion system for the selection of protein variants with increased stability in vivo / Linda Foit and James C.A. Bardwell -- 2. Determining enzyme kinetics via isothermal titration calorimetry / Neil A. Demarse, Marie C. Killian, Lee D. Hansen and Colette F. Quinn -- 3. GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria / Olga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo, George Georgiou and Everett Stone -- 4. Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties / Sarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg and Jordan Jarjour -- 5. TAL effector nuclease (TALEN) engineering / Ting Li and Bing Yang -- 6. In vitro evolution of enzymes / Misha V. Golynskiy, John C. Haugner III, Aleardo Morelli, Dana Morrone and Burckhard Seeling -- 7. Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo / Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington -- 8. Reconstructing evolutionary adaptive paths for protein engineering / Megan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, and Eric A. Gaucher -- 9. Oligonucleotide recombination enabled site-specific mutagenesis in bacteria / Bryan M. Swingle -- 10. FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants / Eric R. Geertsma -- 11. Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes / Hidehiko Hirakawa and Teruyuki Nagamune -- 12. Gene synthesis by assembly of deoxyuridine-containing oligonucleotides / Romualdas Vaisvila and Jurate Bitinaite -- 13. Protein engineering : single or multiple site-directed mutagenesis / Pei-Chung Hsieh and Romualdas Vaisvila -- 14. Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination / Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish -- 15. Promiscuity-based enzyme selection for rational directed evolution experiments / Sandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. Dandekar, Swapan K. Bhattacharjee and Basuthkar J. Rao -- 16. Rational protein sequence diversification by multi-codon scanning mutagenesis / Jia Liu and T. Ashton Cropp -- 17. Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter / Jian-Wei Liu and David L. Ollis -- 18. In vitro directed evolution of enzymes expressed by E. coli in microtiter plates / Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis.
  • 2010 ScienceDirect
    Purich, Daniel L.
    Chapter 1. An Introduction to Enzyme Science -- Chapter 2. Active Sites and their Chemical Properties -- Chapter 3. Fundamentals of Chemical Kinetics -- Chapter 4. Practical Aspects of Measuring Initial Rates and Reaction Parameters -- Chapter 5. Initial-Rate Kinetics of One-Substrate Enzyme-Catalyzed Reactions -- Chapter 6. Initial-Rate Kinetics of Multi-Substrate Enzyme-Catalyzed Reactions -- Chapter 7. Factors Influencing Enzyme Activity -- Chapter 8. Kinetic Behavior of Enzyme Inhibitors -- Chapter 9. Isotopic Probes of Biological Catalysis -- Chapter 10. Probing Fast Enzyme Processes -- Chapter 11. Regulatory Behavior of Enzymes -- Chapter 12. Single-Molecule Enzyme Kinetics -- Chapter 13. Mechanoenzymes: Catalysis, Force Generation and Kinetics.
  • 2002 Wiley
    Bisswanger, Hans; Bubenheim, Leonie.
    Introduction, p. 1-3 -- ch. 1. Multiple Equilibria, p. 5-50 -- ch. 2. Enzyme Kinetics, p. 51-160 -- ch. 3. Methods, p. 161-246.
  • 2011 Wiley
    Alberty, Robert A.
    Also available: Print – v. 53, 2011.
  • 2002 ScienceDirect
    Purich, Daniel L.
    Also available: Print – 2002
  • 2011 Springer Protocols
    Minteer, Shelley D.
    Introduction to the field of enzyme immobilization and stabilization -- Stabilization of enzymes through encapsulation in liposomes -- Micellar enzymology for thermal, pH, and solvent stability -- Lipase activation and stabilization in room-temperature ionic liquids -- Nanoporous silica glass for the immobilization of interactive enzyme systems -- Enzyme stabilization and immobilization by sol-gel entrapment -- Nanoporous gold for enzyme immobilization -- Enzyme stabilization via bio-templated silicification reactions -- The immobilization of enzyme on Eupergit) supports by covalent attachment -- Micellar polymer encapsulation of enzymes -- Enzyme stabilization via cross-linked enzyme aggregates -- Enzyme immobilization in polyelectrolyte microcapsules -- Macroporous poly(GMA-co-EGDMA) for enzyme stabilization -- Enzyme-nanoparticle conjugates for biomedical applications -- Enzyme nanoparticle fabrication: Magnetic nanoparticle synthesis and enzyme immobilization -- Cytochrome C stabilization and immobilization in aerogels -- Kinetic measurements for enzyme immobilization.
  • 2002 CRCnetBASE
    Burns, Richard G.; Dick, Richard P.
    1. Enzyme Activities and Microbiological and Biochemical Processes in Soil / Paolo Nannipieri, Ellen Kandeler, and Pacifico Ruggiero -- 2. Ecology of Microbial Enzymes in Lake Ecosystems / Ryszard Jan Chróst and Waldemar Siuda -- 3. Ecological Significance of Bacterial Enzymes in the Marine Environment / Hans-Georg Hoppe, Carol Arosti, and Gerhard F. Herndl -- 4. Enzymes and Microorganisms in the Rhizosphere / David C. Naseby and James M. Lynch -- 5. Enzymes in the Arbuscular Mycorrhizal Symbiosis / José Manuel García-Garrido, Juan Antonio Ocampo, and Inmaculada García-Romera -- 6. Microbes and Enzymes Associated with Plant Surfaces / Alan P. Thompson and Mark J. Bailey -- 7. Microbial Enzymes in the Biocontrol of Plant Pathogens and Pests / Leonid Cherin and Ilan Chet -- 8. Microbiology and Enzymology of Carbon and Nitrogen Cycling / Robert L. Tate III -- 9. Enzyme and Microbial Dynamics of Litter Decomposition / Robert L. Sinsabaugh, Margaret M. Carreiro, and Sergio Alvarez -- 10. Fungal Communities, Succession, Enzymes, and Decomposition / Annelise H. Kjøller and Sten Struwe -- 11. Enzyme Adsorption on Soil Mineral Surfaces and Consequences for the Catalytic Activity / Hervé Quiquampoix, Sylvie Servagent-Noinville, and Marie-Hélène Baron -- 12. Microbes and Enzymes in Biofilms / Jana Jass, Sara K. Roberts, and Hilary M. Lappin-Scott -- 13. Search for and Discovery of Microbial Enzymes from Thermally Extreme Environments in the Ocean / Jody W. Deming and John A. Baross -- 14. Molecular Methods for Assessing and Manipulating the Diversity of Microbial Populations and Processes / Søren J. Sørensen, Julia R. de Lipthay, Anne Kirstine Müller, Tamar Barkay, Lars H. Hansen, and Lasse Dam Rasmussen -- 15. Bioindicators and Sensors of Soil Health and the Application of Geostatistics / Ken Killham and William J. Staddon -- 16. Hydrolytic Enzyme Activities to Assess Soil Degradation and Recovery / Tom W. Speir and Des J. Ross -- 17. Enzymatic Responses to Pollution in Sediments and Aquatic Systems / Sabine Kuhbier, Hans-Joachim Lorch, and Johannes C.G. Ottow -- 18. Microbial Dehalogenation Reactions in Microorganisms / Lee A. Beaudette, William J. Staddon, Michael B. Cassidy, Marc Habash, Hung Lee, and Jack T. Trevors -- 19. Isolated Enzymes for the Transformation and Det6xification of Organic Pollutants / Liliana Gianfreda and Jean-Marc Bollag -- 20. Enzyme-Mediated Transformations of Heavy Metals/Metalloids: Applications in Bioremediation / Robert S. Dungan and William T. Frankenberger, Jr. -- 21. Enzymes in Soil: Research and Developments in Measuring Activities / M. Ali Tabatabai and Warren A. Dick.
  • 2007 Springer
    Berliner, Lawrence J.; Hemminga, Marcus A.
  • 2012 Springer
    MacNeill, Stuart.
    Composition and Dynamics of the Eukaryotic Replisome: A Brief Overview / Stuart MacNeill -- Evolutionary Diversification of Eukaryotic DNA Replication Machinery / Stephen J. Aves, Yuan Liu and Thomas A. Richards -- The Origin Recognition Complex: A Biochemical and Structural View / Huilin Li and Bruce Stillman -- Archaeal Orc1/Cdc6 Proteins / Stephen D. Bell -- Cdt1 and Geminin in DNA Replication Initiation / Christophe Caillat and Anastassis Perrakis -- MCM Structure and Mechanics: What We Have Learned from Archaeal MCM / Ian M. Slaymaker and Xiaojiang S. Chen -- The Eukaryotic Mcm2-7 Replicative Helicase / Sriram Vijayraghavan and Anthony Schwacha -- The GINS Complex: Structure and Function / Katsuhiko Kamada -- The Pol [alpha]-Primase Complex / Luca Pellegrini -- The Structure and Function of Replication Protein A in DNA Replication / Aishwarya Prakash and Gloria E. O. Borgstahl -- Structural Biology of Replication Initiation Factor Mcm10 / Wenyue Du, Melissa E. Stauffer and Brandt F. Eichman -- Structure and Function of Eukaryotic DNA Polymerase [delta] / Tahir H. Tahirov -- DNA Polymerase [epsilon] / Matthew Hogg and Erik Johansson -- The RFC Clamp Loader: Structure and Function / Nina Y. Yao and Mike O'Donnell -- PCNA Structure and Function: Insights from Structures of PCNA Complexes and Post-translationally Modified PCNA / Lynne M. Dieckman, Bret D. Freudenthal and M. Todd Washington -- The Wonders of Flap Endonucleases: Structure, Function, Mechanism and Regulation / L. David Finger, John M. Atack, Susan Tsutakawa, Scott Classen and John Tainer, et al. -- DNA Ligase I, the Replicative DNA Ligase / Timothy R. L. Howes and Alan E. Tomkinson.
  • NCBI Bookshelf
  • 2012 Springer
    Navard, Patrick.
    This book provides an essential overview of the science of polysaccharides. It both approaches polysaccharides as a polymer class and provides detailed descriptions of most major polysaccharides (cellulose, mannan, xylan, chitin-chitosan, cyclodextrines). Owing to the multidisciplinary character of the European Polysaccharide Network of Excellence (EPNOE), the book describes all main aspects of polysaccharide science and technology (biology, enzymology, physics, chemistry, materials science and processing). Notations and concepts follow a uniform format throughout the whole work in order to create a valuable reference book on the field of polysaccharide science. Owing to the major importance of industry in the EPNOE, concrete applications are also described in detail.
  • 2013 Wiley
    Copeland, Robert Allen.
    "There has been explosive growth in the hunt for new pharmaceutically agents globally. Traditionally, this has been the purview of the pharmaceutical industry, but today, this effort crosses academic, government, and industry laboratories across the world. Enzymes remain the most valued and common of drug targets; hence, a detailed understanding of their interactions with inhibitors is critical to successful drug discovery. This book provides a practical, readable, and comprehensive treatment of these topics that allows scientists to master the art of applied enzymology for drug discovery. The book addresses the opportunities for inhibitor interactions with enzyme targets arising from consideration of the catalytic reaction mechanism; discusses how inhibitors are properly evaluated for potency, selectivity, and mode of action, covers the potential advantages and liabilities of specific inhibition modalities with respect to efficacy in vivo, and provides valuable biochemical insights to help medicinal chemists and pharmacologists most effectively pursue lead optimization. It includes two new chapters, one on the pioneering idea of drug-target residence time fostered by Dr. Copeland, and the second on quantitative biochemistry. Five new appendices are added"--Provided by publisher.
  • 2006 CRCnetBASE
    Lundblad, Roger L.
  • 2008 Springer
    Taniguchi, Naoyuki.
  • 2011 Springer
    Mecham, Robert P.; Parks, William C.
    Matrix metalloproteinases and their inhibitors in turnover and degradation of extracellular matrix / Sean E. Gill and William C. Parks -- Role of cysteine cathepsins in extracellular proteolysis / Dieter Brömme and Susan Wilson -- Cooperation between proteolysis and endocytosis in collagen turnover / Thomas H. Bugge and Niels Behrendt -- Pericellular proteolysis / Judith S. Bond, Timothy R. Keiffer, and Qi Sun -- Triple helicase activity and the structural basis of collagenolysis / Hideaki Nagase and Robert Visse -- Structural basis of extracellular matrix interactions with matrix metalloproteinases / Steven R. Van Doren -- The role of matrix metalloproteinases in cellular invasion and metastasis / Elena I. Deryugina and James P. Quigley -- Degradation of bone and the role of osteoclasts, bone lining cells and osteocytes / Vincent Everts and Paul Saftig -- Consequences of elastolysis / A. McGarry Houghton, Majd Mouded, and Steven D. Shapiro.
    Also available: Print – 2011
  • 2009 Springer Protocols
    Artym, Vira V.; Even-Ram, Sharona; Streuli, Charles H.
    Retroviral delivery of ECM genes / Vitali alexeev and Olga Igoucheva -- Tissue-specific KO of ECM proteins / Mara Brancaccio, Emila Turco, and Emilio Hirsch -- Recombinant collagen trimers from insect cells and yeast / Johanna Myllyharju -- Eukaryotic expression and purification of recombinant extracellular matrix proteins carrying the Strep II tag / Neil Smyth ... [et al.] -- Preparation of recombinant fibronectin fragments for functional and structural studies / David Staunton ... [et al.] -- Quantitative determination of collagen cross-links / Nicholas C. Avery, Trevor J. Sims, and Allen J. Bailey -- ECM macromolecules : height-mapping and nano-mechanics using atomic force microscopy / Nigel W. Hodson, Cay M. Kielty, and Michael J. Sherratt -- Atomic force microscopy measurements of intermolecular binding forces / Gradimir N. Misevic, Yannis Karamanos, and Nikola J. Misevic -- Mass-mapping of ECM macromolecules by scanning transmission electron microscopy / Michael J. Sherratt ... [et al.] -- Chemical microscopy of biological samples by dynamic mode secondary ion mass spectrometry / Gradimir N. Misevic ... [et al.] -- ECM macromolecules : rotary shadowing and transmission electron microscopy / Michael J. Sherratt ... [et al.] -- Using self-assembled monolayers to pattern ECM proteins and cells on substrates / Emanuele Ostuni ... [et al.] -- Solid phase assays for studying ECM protein-protein interactions / A. Paul Mould -- Cell adhesion assays / Martin J. Humphries -- ECM degradation assays for analyzing local cell invasion / Vira V. Artym, Kenneth M. Yamada, and Susette C. Mueller -- Fluorescence-based assays for in vitro analysis of cell adhesion and migration / Paola Spessotto ... [et al.] -- Fibrin gel model for assessment of cellular contractility / Sharona Even-Ram -- Fluorescent labeling techniques for investigation of fibronectin fibrillogenesis (labeling fibronectin fibrillogenesis) / Roumen Pankov and Albena Momchilova -- Stromagenesis during tumorigenesis : characterization of tumor-associated fibroblasts and stroma-derived 3D matrices / Remedios Castelló-Cros and Edna Cukierman -- Tissue recombinants to study extracellular matrix targeting to basement membranes / Patricia Simon-Assmann ... [et al.] -- ECM and FGF-dependent assay of embryonic SMG epithelial morphogenesis : investigating growth factor/matrix regulation of gene expression during submandibular gland development / Ivan T. Rebustini and Matthew P. Hoffman -- Analyzing how cell adhesion controls mammary gland function by transplantation of embryonic mammary tissue from knockout mice / Teresa C.M. Klinowska and Charles H. Streuli -- Characterizing ECM production by cells encapsulated in hydrogels / Iossif A. Strehin and Jennifer H. Elisseeff -- Tissue engineering and cell-populated collagen matrices / Paul D. Kemp.
  • 2010 Springer
    Kikuchi, Yo; Rykova, Elena Y.
    Extracellular DNA as Matrix Component in Microbial Biofilms / Wen-Chi Chiang and Tim Tolker-Nielsen -- Gene Transfer Agents and Defective Bacteriophages as Sources of Extracellular Prokaryotic DNA / Andrew S. Lang and J. Thomas Beatty -- Roles of Extracellular DNA in Bacterial Ecosystem / Katsuji Tani and Masao Nasu -- Stable Extracellular DNA: A Novel Substrate for Genetic Engineering that Mimics Horizontal Gene Transfer in Nature / Shinya Kaneko and Mitsuhiro Itaya -- Extracellular Nucleic Acids of the Marine Phototrophic Bacterium Rhodovulum sulfidophilum and Related Bacteria: Physiology and Biotechnology / Yo Kikuchi -- Systemic RNAi in C. elegans from the Viewpoint of RNA as Extracellular Signals / Lisa Timmons -- Circulating Nucleic Acids in Health and Disease / E. Y. Rykova, P. P. Laktionov and V. V. Vlassov -- Circulating MicroRNAs in Cancer / Patrick S. Mitchell and Muneesh Tewari -- Biology and Diagnostic Applications of Cell-Free Fetal Nucleic Acids in Maternal Plasma / Jason C. H. Tsang and Y. M. Dennis Lo -- The Biology of Circulating Nucleic Acids in Plasma and Serum (CNAPS) / P. B. Gahan and M. Stroun -- Modulation and Regulation of Gene Expression by CpG Oligonucleotides / Dennis Klinman and Sven Klaschik -- Immune Recognition of Nucleic Acids and Their Metabolites / Shohei Koyama, Shizuo Akira and Ken J. Ishii.
  • 2012 Springer
    Kotb, Essam.
  • 2012 CRCnetBASE
    Crozier, Alan; Spencer, Jeremy P .E.
    Bioavailability of flavanones / Mireia Urpi-Sarda ... [et al.] -- Bioavailability of dietary monomeric and polymeric flavan-3-ols / Alan Crozier, Michael N. Clifford, and Daniele Del Rio -- Anthocyanins : understanding their absorption and metabolism / Ronald L. Prior -- Bioavailability of flavonols and flavones / Mariusz Konrad Piskula, Kaeko Murota, and Junji Terao -- Bioavailability of isoflavones in humans / Aedín Cassidy, José Peñalvo, and Peter Hollman -- Dietary hydroxycinnamates and their bioavailability / Angelique Stalmach, Gary Williamson, and Mike Clifford -- Bioavailability of dihydrochalcones / Elke Richling -- Occurrence, bioavailability, and metabolism of resveratrol / Paola Vitaglione, Stefano Sforza, and Daniele Del Rio -- Bioavailability and metabolism of ellagic acid and ellagitannins / Mar Larrosa ... [et al.] -- Colon-derived microbial metabolites of dietary phenolic compounds / Anna-Marja Aura -- Synthesis of dietary phenolic metabolites and isotopically labeled dietary phenolics / Denis Barron ... [et al.] -- Interactions of flavan-3-ols within cellular signaling pathways / Cesar G. Fraga and Patricia I. Oteiza -- Flavonoids and vascular function / Ana Rodriguez-Mateos and Jeremy P.E. Spencer -- Effects of flavonoids on the vascular endothelium : what is known and what is next? / Antje R. Weseler and Aalt Bast -- Green tea flavan-3-ols and their role in protecting against Alzheimer's and Parkinson's disease pathophysiology / Orly Weinreb ... [et al.] -- Flavonoids and neuroinflammation / David Vauzour and Katerina Vafeiadou -- Effects of flavonoids on cognitive performance / Shibu M. Poulose and Barbara Shukitt-Hale -- Flavonoids and oral cancer / Thomas Walle -- Flavonoids and cancer-effects on dna damage / Piyawan Sitthiphong ... [et al.].
  • 2010 ClinicalKey
    Halperin, M. L.; Goldstein, Marc B.; Kamel, Kamel S.
    Principles of acid-base physiology -- Tools to use to diagnose acid-base disorders -- Metabolic acidosis: clinical approach -- Metabolic acidosis due to a deficit of NaHCO₃ -- Ketoacidosis -- Metabolic acidosis: acid gain types -- Metabolic alkalosis -- Respiratory acid-base disturbances -- Sodium and waterphysiology -- Hyponatremia -- Hypernatremia -- Polyuria -- Potassium physiology -- Hypokalemia -- Hyperkalemia -- Hyperglycemia.
    Also available: Print – 2010
  • 2007 Springer
    Ishihara, Hironori; Giesecke, A. H.
  • 2008 ScienceDirect
    Brand, Ludwig; Johnson, Michael L.
    A method in enzymology for measuring hydrolytic activities in live cell environments / Beverly Z. Packard and Akira Komoriya -- Heterogeneity of fluorescence determined by the method of area-normalized time-resolved emission spectroscopy / N. Periasamy -- Multiparametric probing of microenvironment with solvatochromic fluorescent dyes / Andrey S. Klymchenko and Alexander P. Demchenko -- Site-selective red-edge effects / Alexander P. Demchenko -- Fluorescence approaches to quantifying biomolecular interactions / Catherine A. Royer and Suzanne F. Scarlata -- Forster resonance energy transfer measurements of transmembrane helix dimerization energetics / Mikhail Merzlyakov and Kalina Hristova -- Application of single-molecule spectroscopy in studying enzyme kinetics and mechanism / Jue Shi ... [et al.] -- Ultrafast fluorescence spectroscopy via upconversion : applications to biophysics / Jianhua Xu and Jay R. Knutson -- Use of fluorescence resonance energy transfer (FRET) in studying protein-induced DNA bending / Anatoly I. Dragan and Peter L. Privalov --Fluorescent pteridine probes for nucleic acid analysis / Mary E. Hawkins -- Single-molecule fluorescence methods for the analysis of RNA folding and ribonucleoprotein assembly / Goran Pljevaljčić and David P. Millar -- Using fluorophore-labeled oligonucleotides to measure affinities of protein-DNA interactions / Brian J. Anderson ... [et al.] -- Identifying small pulsatile signals within noisy data : a fluorescence application / Michael L. Johnson ... [et al.] -- Determination of zinc using carbonic anhydrase-based fluorescence biosensors / Rebecca Bozym ... [et al.] -- Instrumentation for fluorescence-based fiber optic biosensors / Richard B. Thompson ... [et al.]
    Also available: Print – 2008
  • 2014 Springer
    Fili, Natalia; Toseland, Christopher P.
    This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.
  • 2014 Springer Protocols
    Newman, Robert H.; Ni, Qiang; Zhang, Jin.
    Design and application of genetically encodable biosensors based on fluorescent proteins / Robert H. Newman and Jin Zhang -- Introduction to fluorescence imaging techniques geared towards biosensor applications / J. Goedhart, Mark A. Hink, and Kees Jalink -- Quantitative measurement of Ca²⁺ and Zn²⁺ in mammalian cells using genetically encoded fluorescent biosensors / J. Genevieve Park and Amy E. Palmer -- Detecting cAMP with an epac-based FRET sensor in single living cells / J. Klarenbeek and Kees Jalink -- Analysis of compartmentalized cAMP : a method to compare signals from differently targeted FRET reporters / Alessandra Stangherlin ... [et al.] -- Genetically encoded fluorescent biosensors for live cell imaging of lipid dynamics / Moritoshi Sato -- Live-cell imaging of cytosolic NADH-NAD⁺ redox state using a genetically encoded fluorescent biosensor / Yin Pun Hung and Gary Yellen -- Measuring membrane voltage with microbial rhodopsins / Adam E. Cohen and Daniel R. Hochbaum -- Imaging the activity of Ras superfamily GTPase proteins in small subcellular compartments in neurons / Ana F. Oliveira and Ryohei Yasuda -- Imaging kinase activity at protein scaffolds / Maya T. Kunkel and Alexandra C. Newton -- Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells / Sohum Mehta and Jin Zhang -- Genetically encoded FRET indicators for live-cell imaging of histone acetylation / Kazuki Sasaki and Minoru Yoshida -- Genetically encoded fluorescent biosensors for live-cell imaging of MT1-MMP protease activity / Mingxing Ouyang, Shaoying Lu, and Yingxiao Wang -- Biosensor imaging in brain slice preparations / Marina Polito, Pierre Vincent, and Elvire Guiot -- Optical calcium imaging using DNA-encoded fluorescence sensors in transgenic fruit flies, Drosophila melanogaster / Shubham Dipt, Thomas Riemensperger, and André Fiala -- Multiparameter live cell imaging approach to monitor cyclic AMP and protein kinase a dynamics in parallel / Nwe-Nwe Aye-Han and Jin Zhang -- FRET and BRET-based biosensors in live cell compound screens / Katie Herbst Robinson, Jessica R. Yang, and Jin Zhang -- Integrating fluorescent biosensor data using computational models / Eric C. Greenwald, Renata K. Polanowska-Grabowska, and Jeffrey J. Saucerman.
  • 2011 Springer
    O'Connor, Vincent; Wyttenbach, Andreas.
    Folding for the Synapse addresses the current view on how protein folding and misfolding, controlled by molecular chaperones, contribute to synapse function and dysfunction. Molecular chaperones have been studied in relation to de novo protein folding, but there is increasing awareness that chaperone function is required for the regulation of protein dynamics when functioning physiologically as an isolated moiety or part of a protein complex. This book will introduce both important concepts of folding machineries and give examples of the biological relevance of further chaperone functions.
  • 2011 Springer
    Chang, Rowen J. Y.; Ventura, Salvador.
    1. Oxidative folding: coupling conformational folding and disulfide formation -- 2. The case of oxidative folding of ribonuclease A: factors impacting fold maturation of ER-processed proteins -- 3. Cystine knot folding in cyclotides -- 4. In vitro folding of single/double chain insulins and related proteins -- 5. Unfolding and refolding of disulfide proteins using the method disulfide scrambling -- 6. Oxidative protein folding with small molecules -- 7. Protein disulfide isomerase and the catalysis of oxidative protein folding -- 8. Allosteric disulfide bonds -- 9. The problem of expression of multidisulfide bonded recombinant proteins in E. coli -- 10. NMR-spectroscopic investigation of disulfide dynamics in unfolded states of proteins -- 11. A half-century of oxidative folding and protein disulfphide formation -- Index.
  • 2009 Springer
    Maiese, Kenneth.
    FOXO1, T-cell trafficking and immune responses / Florent Carrette, Stéphanie Fabre and Georges Bismuth -- FOXP3 and its role in the immune system / Chang H. Kim -- Molecular regulation of cellular immunity by FOXP3 / Alicia N. McMurchy ... [et al.] -- The biology of FOXP3 : a key player in immune suppression during infections, autoimmune diseases and cancer / Frances Mercer and Derya Unutmaz -- The cooperative roles of FOXC1 and FOXC2 in cardiovascular development / Tsutomu Kume -- FOXO proteins and cardiac pathology / Albert Wong and Elizabeth A. Woodcock -- The forkhead transcription factors play important roles in vascular pathology and immunology / Xiao-Feng Yang ... [et al.] -- Regulatory T cells, FOXP3 and atherosclerosis / Michal Entin-Meer, Arnon Afek and Jacob George -- FOXP genes, neural development, speech and language disorders / Hiroshi Takahashi, Kaoru Takahashi and Fu-Chin Liu -- Pathophysiological relevance of forkhead transcription factors in brain ischemia / Kohji Fukunaga and Norifumi Shioda -- New insights for FOXO and cell-fate decision in HIV infection and HIV associated neurocognitive disorder / Min Cui ... [et al.] -- FOXP3+ regulatory T-cells in chronic kidney disease : molecular pathways and clinical implications / Pascal Meier -- Transcriptional role of FOXO1 in drug resistance through antioxidant defense systems / Tomoko Goto and Masashi Takano -- FOXP3 expressing regulatory T-cells in allergic disease / Kayhan T. Nouri-Aria -- Human clinical phenotype associated with FOXN1 mutations / Claudio Pignata, Anna Fusco and Stefania Amorosi -- FOXL2 : at the crossroads of female sex determination and ovarian function / Bérénice A. Benayoun ... [et al.] -- FOXO transcription factors : from cell fate decisions to regulation of human female reproduction / Jan J. Brosens, Miranda S.C. Wilson and Eric W.-F. Lam -- The "O" class : crafting clinical care with FOXO transcription factors / Kenneth Maiese ... [et al.].
    Also available: Print – 2009
  • 2013 Springer
    Banerji, Anirban.
    Introduction -- Characterization of Protein-Protein Interfaces, Considering Surface-Roughness and Local Shape -- Adhesion on Protein (and Other Rough Biomolecular) Surfaces.
  • 2013 Springer
    Banerji, Anirban.
    Introduction -- Characterization of Protein-Protein Interfaces, Considering Surface-Roughness and Local Shape -- Adhesion on Protein (and Other Rough Biomolecular) Surfaces.
  • 2006 Springer
    Sonntag, C. von.
  • 2012 Springer
    Barciszewski, Jan; Erdmann, V. A.
    RNAissance / Juergen Brosius -- Nucleic Acids as Therapeutics / Saraswat Pushpendra, Pareek Arvind and Bhandari Anil -- Thio Effects as a Tool for Mechanistic Studies of the Cleavage of RNA Phosphodiester Bonds: The Chemical Basis / Mikko Ora, Tuomas Lönnberg and Harri Lönnberg -- Off-Target Effects and Safety Aspects of Phosphorothioate Oligonucleotides / Martina Stessl, Christian R. Noe and Johannes Winkler -- Oligonucleotide Conjugates: Rationale, Synthesis, and Applications / Yashveer Singh, Pierre Murat, Nicolas Spinelli and Eric Defrancq -- CRISPR: A Bacterial Immunity System Based on Small RNAs / Rolf Wagner and Ümit Pul -- Antisense Technology: From Unique Laboratory Tool to Novel Anticancer Treatments / Christine Di Cresce, Colin Way, Mateusz Rytelewski, Saman Maleki Vareki and Supritha Nilam, et al. -- Antisense-Mediated Reduction of Eukaryotic Noncoding RNAs / Xue-hai Liang, Timothy A. Vickers and Stanley T. Crooke -- Antisense Oligonucleotides in the Treatment of Malignant Gliomas / Gerardo Caruso, Mariella Caffo, Giuseppe Raudino, Federica Raudino and Mario Venza, et al. -- Natural Antisense Transcripts Mediate Regulation of Gene Expression / Marco Magistri and Mohammad Ali Faghihi -- Activation and Deactivation of Antisense and RNA Interference Function with Light / Jeane M. Govan and Alexander Deiters -- Ribozymes as Molecular Biology Reagents / Sanchita Bhadra, Arti Pothukuchy and Andrew Ellington -- RNA Technologies for Mitochondrial Genetics / André Dietrich, Katarzyna Rolle, Marta Gabryelska, Eliza Wyszko and Romain Val, et al. -- RNA-Based Therapies for Inherited Metabolic Diseases / Belén Pérez, Magdalena Ugarte and Lourdes R. Desviat -- RNA-Cleaving DNA Enzymes and Their Potential Therapeutic Applications as Antibacterial and Antiviral Agents / P. I. Pradeepkumar and Claudia Höbartner -- Hammerhead Ribozymes Against Virus and Viroid RNAs / Alberto Carbonell, Ricardo Flores and Selma Gago -- Suppression of Hepatitis C Viral Genome Replication with RNA-Cleaving Deoxyribozyme / Dal-Hee Min and Dong-Eun Kim -- Design of Synthetic shRNAs for Targeting Hepatitis C: A New Approach to Antiviral Therapeutics / Brian H. Johnston and Qing Ge -- The Diverse Active Sites in Splicing, Debranching, and MicroRNA Processing Around RNA Phosphodiester Bonds / Sourav Kumar Dey, Eduardo Paredes, Molly Evans and Subha R. Das -- MicroRNAs and Their Antagonists as Novel Therapeutics / Jeppe Vinther, Jakob Lewin Rukov and Noam Shomron -- microRNAs in Human Diseases and Viral Infections / Alessandra Mescalchin and Tobias Restle -- Dysregulation of MicroRNA Expression and Human Diseases? / To-Ha Thai -- Noncoding RNAs: Identification of Cancer-Associated MicroRNAs / Muller Fabbri and George A. Calin -- Targeting Non-coding RNAs for Cancer Therapy / Satya K. Kota and Savithri Balasubramanian -- MicroRNA Pathways in Drosophila / Geetanjali Chawla and Nicholas S. Sokol -- Viroids: The Smallest Known Infectious Agents Cause Accumulation of Viroid-Specific Small RNAs / Jaroslav Matoušek, Detlev Riesner and Gerhard Steger.
  • 2011
    Chuan, Peiying; Dunn, Alexander Robert; Harbury, Pehr A. B.; Herschlag, Daniel; Spudich, James A.
    Myosins are a superfamily of molecular motors that couple the chemical energy derived form adenosine triphosphate (ATP) hydrolysis to precise mechanical movements along the filamentous cytoskeletal protein actin. There exist more than 20 distinct classes of myosins within this superfamily, and their specific biophysical and biochemical properties likely allow them to function accordingly in vivo. My research has focused on understanding the how the structural and kinetic characteristics of a particular myosin, myosin VI, relate to its proposed trafficking and tethering functions. In the first section of this dissertation, I perform single molecule studies on the contribution of an unexpected structural domain in the myosin VI tail to the efficiency of processive stepping, especially against external forces. I additionally use optical trapping to examine the load-dependent kinetics of the motor in the second section, and propose that external load in vivo likely regulates myosin VI's dual functions as cellular transporter and cytoskeletal anchor. The specific cellular cargoes as well as oligomerization states of different myosins are defined by their tail domain, which is the most divergent structural region among myosin classes. Myosin VI, shown to be monomeric when purified in its native form, likely dimerizes upon cargo binding. As most in vitro work on myosin VI so far have been performed on a truncated, artificially forced myosin VI dimer, I discuss in the third section steps taken toward achieving a more native, biochemically reconstituted full-length myosin VI molecule bound to its bona fide cargo. Having gained a better understanding of the biophysics, kinetics and biochemistry of myosin at the single molecule level, I moved on to studying myosin function at the cellular level. In the forth section of this dissertation, I examine the effects of a familial hypertrophic cardiomyopathy (FHC) mutation, R403Q in cardiac myosin, on the contractile function of single mouse cardiomyocytes. I find that the functional effects of the R403Q mutation in the diseased FHC heart are manifest at the level of cardiomyocytes as well, suggesting that these effects are cell autonomous. From these studies, we can better appreciate the structural and kinetic properties of myosin VI that make it uniquely suited for its functions. This knowledge is also beneficial in aiding the understanding of other molecular motors that may employ similar mechanisms. We are now also aware of the cell-autonomy of particular dysfunctions exhibited by diseased R403Q FHC hearts, and are better poised to understand the complex disorder as a whole.
  • 2006 Springer
    2006 MyiLibrary
    Cheng, Jing; Xing, Wan-Li.
  • 2007 Springer
    Leung, Po Sing.
  • 2011 Springer
    Nettleship, Joanne E.; Owens, Raymond J.
    Glycoproteomics in health and disease / Weston B. Struwe, Eoin F.J. Cosgrave, Jennifer C. Byrne, Radka Saldova, and Pauline M. Rudd -- Glyco-engineering of Fc Glycans to enhance the biological functions of therapeutic IgGs / T. Shantha Raju, David M. Knight, and Robert E. Jordan -- Bioinformatics databases and applications available for glycobiology and glycomics / René Ranzinger, Kai Maass, and Thomas Lütteke -- Lectin microarrays: Simple tools for the analysis of complex glycans / Lakshmi Krishnamoorthy and Lara K. Mahal -- The application of high throughput mass spectrometry to the analysis of glycoproteins / Sasha Singh, Morten Thaysen Andersen, and Judith Jebanathirajah Steen -- Solutions to the glycosylation problem for low- and high-throughput structural glycoproteomics / Simon J. Davis and Max Crispin -- Role of glycoproteins in virus-human cell interactions / Thomas A. Bowden and Elizabeth E. Fry.
  • 2010 ScienceDirect
    Fukuda, Minoru.
    Also available: Print – 2010
  • 2006 CRCnetBASE
    Feng, Li; Prestwich, Glenn D.
  • 2014 Springer
    Abmayr, Susan M.; Workman, Jerry L.
    Chromatin is the combination of DNA and proteins that make up the genetic material of chromosomes. It is essential for packaging DNA, regulation of gene expression, DNA replication and repair. The audience for this book includes newly established scientists, graduate students and scientists seeking detailed overviews of various aspects of chromatin structure and function to gain entry into this field. The chapters in this book give a strong, updated groundwork about the fundamentals of chromatin which are essential to understand epigenetics. Some the fundamentals that this book covers include the structure and biochemistry of chromatin and the enzyme complexes that manage it.
  • 2007 Springer
    Buxbaum, Engelbert.
    Pt. I. Protein structure. Amino acids ; Protein structure ; Purification and characterisation of proteins ; Protein sequencing -- Pt. II. Enzymes. Enzymes are biocatalysts ; Enzyme kinetics ; Haemoglobin and myoglobin: co-operativity ; Enzyme kinetics: special cases -- Pt. III. Special proteins. Prion proteins and prion diseases ; Immunoproteins ; Cell skeleton ; Motor proteins and movement ; Cell-cell interactions ; Aiding in folding: molecular chaperones and chaperonins -- Pt. IV. Membrane transport. Transport of proteins into mitochondria ; Intracellular protein transport ; Transport of solutes across membranes -- Appendix. A.1. Solutions for the exercise ; A.2. Short biographies of scientists mentioned in this book ; A.3. List of symbols ; A.4. Greek alphabet ; A.5. The genetic code ; A.6. Acronyms.
  • 2012 Springer
    Fuxreiter, Monika; Tompa, Peter.
    Fuzzy complexes : a more stochastic view of protein function / Monika Fuxreiter and Peter Tompa -- Dynamic fuzziness during linker histone action / Steven J. McBryant and Jeffrey C. Hansen -- Intrinsic protein flexibility in regulation of cell proliferation : advantages for signaling and opportunities for novel therapeutics / Ariele Viacava Follis, Charles A. Galea, and Richard W. Kriwacki -- Interplay between protein order, disorder and oligomericity in receptor signaling / Alexander B. Sigalov -- Consequences of fuzziness in the NFB/IB interaction / Elizabeth A. Komives -- Roles for intrinsic disorder and ̂fuzziness in generating context specific function in ultrabithorax : a hox transcription factor / Sarah E. Bondos and Hao Ching Hsiao -- Molecular recognition by the EWS transcriptional activation domain / Kevin A.W. Lee -- The measles virus NTAILXD complex : an illustrative example of fuzziness / Sonia Longhi -- Fuzziness in the core of the human pathogenic viruses HCV and HIV / Roland Ivanyi Nagy and Jean Luc Darlix -- Structural disorder and protein elasticity / Sarah Rauscher and Røgis Pomès -- "Fuzziness" in the cellular interactome: a historical perspective / G. Rickey Welch.
    Also available: Print – 2012
  • 2013 ScienceDirect
    Conn, P. Michael.
    The protein local optimization program and g-protein-coupled receptors:loop restoration and applications to homology modeling -- Modeling active GPCR conformations -- Molecular modeling of adenosine receptors -- Predicting g-protein-coupled receptors families using differet physiochemical properties and pseudo amino acid composition -- Computational design and experimental characterization of GPCR segment models -- G-protein-coupled receptor interaction with small GTPases -- Quantification of receptor tyrosine kinase activiation and transactivation by G-protein-coupled receptors using spatial intensity distribution analysis (SplDA) -- NMR modeling for detection of small molecule binding to RGS4 -- Group II activators of g-protein signaling: monitoring the interaction of G[alpha] with G-protein motif in the intact cell -- Homogenous time-resolved fluorescence assay to probe folded G protein-coupled receptors -- cAMP biosensors applied in molecular pharmacological studies of G protein-coupled receptors -- Rhodospin-lipid intereactions studied by NMR -- Biasing the parathyroid hormone receptor: relating in vitro ligand efficacy to in vivo biological activity -- Application of Monte Carlo-based receptor ensemble docking to virtual screening for GPCR ligands -- From heptahelical bundle to hits from the haystack: structure-based virtual screening for GPCR ligands -- Kinetics and dynamics in the G protein-coupled receptor signaling cascade -- Magic angle spinning nuclear magnetic resonance spectroscopy fo G protein-coupled receptors -- Posttranslation modification of G protein-coupled receptor in relationship to biased agonism -- Protein S-nitrosylation measurement.
  • 2013 ScienceDirect
    Conn, P. Michael.
    This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain. This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain.
  • 2013 ScienceDirect
    Conn, P. Michael.
    Section 1. GPCR trafficking. Therapeutic rescue of misfolded/mistrafficked mutants : automation-friendly high-throughput assays for identification of pharmacoperone drugs of GPCRs / David C. Smithson, Jo Ann Janovick, and P. Michael Conn ; Trafficking of the follitropin receptor / Alfredo Ulloa-Aguirre, James A. Dias, George Bousfield, Ilpo Huhtaniemi, and Eric Reiter ; Single-molecule imaging technique to study the dynamic regulation of GPCR function at the plasma membrane / B.E. Snaar-Jagalska, A. Cambi, T. Schmidt, and S. de Keijzer ; GPCR oligomerization and receptor trafficking / Richard J. Ward, Tian-Rui Xu, and Graeme Milligan ; [Beta]-arrestins and G protein-coupled receptor trafficking / Dong Soo Kang, Xufan Tian, and Jeffrey L. Benovic ; Tracking cell surface mobility of GPCRs using [alpha]-bungarotoxin-linked fluorophores / Saad Hannan, Megan E. Wilkins, Philip Thomas, and Trevor G. Smart ; Regulatory mechanism of G protein-coupled receptor trafficking to the plasma membrane : a role for mRNA localization / Kusumam Joseph, Eleanor K. Spicer, and Baby G. Tholanikunnel ; Dissecting trafficking and signaling of atypical chemokine receptors / Elena Borroni, Cinzia Cancellieri, Massimo Locati, and Raffaella Bonecchi -- Section 2. Trafficking motifs. Systematic and quantitative analysis of G protein-coupled receptor trafficking motifs / Carl M. Hurt, Vincent K. Ho, and Timothy Angelotti ; Identification of endoplasmic reticulum export motifs for G protein-coupled receptors / Guangyu Wu ; Amino acid residues of G-protein-coupled receptors critical for endoplasmic reticulum export and trafficking / Motonao Nakamura, Daisuke Yasuda, Nobuaki Hirota, Teruyasu Yamamoto, Satoshi Yamaguchi, Takao Shimizu, and Teruyuki Nagamune -- Section 3. GPCR oligomerization. G-protein-coupled heteromers : regulation in disease / Ivone Gomes, Achla Gupta, and Lakshmi A. Devi ; Hetero-oligomerization and specificity changes of G protein-coupled purinergic receptors : novel insight into diversification of signal transduction / Tokiko Suzuki, Kazunori Namba, Natsumi Mizuno, and Hiroyasu Nakata ; Bimolecular fluorescence complementation analysis of G protein-coupled receptor dimerization in living cells / Karin F.K. Ejendal, Jason M. Conley, Chang-Deng Hu, and Val J. Watts ; G protein-coupled receptor heterodimerization in the brain / Dasiel O. Borroto-Escuela, Wilber Romero-Fernandez, Pere Garriga, Francisco Ciruela, Manuel Narvaez, Alexander O. Tarakanov, Miklós Palkovits, Luigi F. Agnati, and Kjell Fuxe ; Experimental strategies for studying G protein-coupled receptor homo- and heteromerization with radioligand binding and signal transduction methods / Roberto Maggio, Cristina Rocchi, and Marco Scarselli ; Analysis of GPCR dimerization using acceptor photobleaching resonance energy transfer techniques / Marta Busnelli, Mario Mauri, Marco Parenti, and Bice Chini ; Techniques for the discovery of GPCR-associated protein complexes / Avais Daulat, Pascal Maurice, and Ralf Jockers ; Expression, purification, and analysis of G-protein-coupled receptor kinases / Rachel Sterne-Marr, Alison I. Baillargeon, Kevin R. Michalski, and John J.G. Tesmer ; Modern methods to investigate the oligomerization of glycoprotein hormone receptors (TSHR, LHR, FSHR) / Marco Bonomi and Luca Persani.
  • 2002 ScienceDirect
    Hildebrandt, John D.; Iyengar, Ravi.
    Also available: Print – 2002
  • 2002 ScienceDirect
    Hildebrandt, John D.; Iyengar, Ravi.
    Also available: Print – 2002
  • 2002 ScienceDirect
    Hildebrandt, John D.; Iyengar, Ravi.
    Also available: Print – 2002
  • 2014 Springer Protocols
    Stevens, Craig W.
    G protein-coupled receptors : research and methods in the post-genomic era / Craig W. Stevens -- Examining the dynamic evolution of G protein-coupled receptors / Claudia Stäubert, Diana Le Duc, and Torsten Schöneberg -- Functional properties of virus-encoded and virus-regulated G protein-coupled receptors / Katja Spiess and Mette M. Rosenkilde -- Discovery and function of the very large G protein-coupled receptor / Perrin C. White and D. Randy McMillan -- Functional evolution of opioid family G protein-coupled receptors / Eyal Vardy, Craig W. Stevens, and Bryan L. Roth -- Investigation of G protein-coupled receptor function and regulation using antisense / Jonathon Willets and Craig Nash -- Signalosome profiling reveals allosteric interactions between G protein-coupled receptors / Katrin Altosaar ... [et al.] -- Use of optogenetic approaches to control intracellular signaling of G protein-coupled receptors / Olivia A. Masseck, Melanie D. Mark, and Stefan Herlitze -- Viral infection for G protein-coupled receptor expression in eukaryotic cells / Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi -- Using in vitro mutagenesis to characterize structure- function relationships in G protein-coupled receptors / Gregory W. Sawyer and Frederick J. Ehlert -- CRE Luc Mouse model for bioimaging ligand activation of G protein-coupled receptors / H. Gregory Polites and Kyriakos D. Economides -- Inducing conformational changes in G protein-coupled receptors by domain coupling / Hamiyet Unal and Sadashiva S. Karnik -- Rescue of defective G protein-coupled receptor function by intermolecular cooperation / Ashutosh Trehan ... [et al.] -- Design of super-arrestins for gene therapy of diseases associated with excessive signaling of G protein-coupled receptors / Vsevolod V. Gurevich and Eugenia V. Gurevich -- Importance of G protein-coupled receptor genetics in clinical medicine / Ramakrishna Devaki -- Pharmacogenomics of G protein-coupled receptor signaling and other pathways in essential hypertension / Pedro A. Jose ... [et al.] -- Exon sequencing of G protein-coupled receptor genes and perspectives for disease treatment / Angeliki Karamitri and Ralf Jockers -- Gene therapy using G protein-coupled receptors for the treatment of cardiovascular disease / Dario Leosco ... [et al.] -- Novel mechanisms of G protein-coupled receptor oligomer and ion channel Iiteractions in nociception / Ajay S. Yekkirala -- Discovery and characterization of leucine-rich repeat-containing G protein-coupled receptors / Marie-Isabelle Garcia, Valeria Fernandez-Vallone, and Gilbert Vassart -- Role of G protein-coupled receptors in adult neurogenesis / Katie M. Collette, Van A. Doze, and Dianne M. Perez.
  • 2014 Springer
    Filizola, Marta.
    G protein-coupled receptors (GPCRs) are heptahelical transmembrane receptors that convert extra-cellular stimuli into intra-cellular signaling, and ultimately into biological responses. Since GPCRs are natural targets for approximately 40% of all modern medicines, it is not surprising that they have been the subject of intense research. Notwithstanding the amount of data generated over the years, discovering ligands of these receptors with optimal therapeutic properties is not straightforward and has certainly been hampered for years by the lack of high-resolution structural information about these receptors.--Back cover.
    Also available: Print – 2014
  • 2007 Springer
    Enna, S. J.; Möhler, Hanns.
  • 2010 Springer
    Hökfelt, T.
    Galanin : a multitalented neuropeptide / T. Hökfelt and K. Tatemoto -- Galanin, galanin receptors, and drug targets / K. Mitsukawa, X. Lu, and T. Bartfai -- Galanin acts as a trophic factor to the central and peripheral nervous systems / S-A. Hobson ... [et al.] -- Galanin and spinal pain mechanisms : past, present, and future / Xiao-Jun Xu, Tomas Hökfelt, and Zsuzsanna Wiesenfeld-Hallin -- Galanin family of peptides in skin function / J.W. Bauer ... [et al.] -- Galanin in glia : expression and potential roles in the CNS / Helmut Butzkueven and Andrew L. Gundlach -- Galanin and the neuroendocrine axes / Istvan Merchenthaler -- Galanin and consummatory behavior : special relationship with dietary fat, alcohol, and circulating lipids / Jessica R. Barson, Irene Morganstern, and Sarah F. Leibowitz -- Neurochemical modulation of central cardiovascular control : the integrative role of galanin / Zaida Díaz Cabiale ... [et al.] -- Galanin impairs cognitive abilities in rodents : relevance to Alzheimer's disease / J.N. Crawley -- Neuroprotective role for galanin in Alzheimer's disease / Scott E. Counts ... [et al.] -- Galanin, galanin receptor subtypes, and depression-like behaviour / Eugenia Kuteeva ... [et al.] -- Galanin and epilepsy / J.T. Lerner, R. Sankar, and A.M. Mazarati -- Galanin and addiction / Marina R. Picciotto -- Galanin systems and ischemia : peptide and receptor plasticity in neurons and oligodendroglial precursors / Pei-Juan Shen and Andrew L. Gundlach -- The galanin system in cancer / I. Rauch and Barbara Kofler -- Galanin systems in non-mammalian vertebrates with special focus on fishes / Elsie Tachie Mensah, Helene Volkoff, and Suraj Unniappan -- Galanin-like peptide : neural regulator of energy homeostasis and reproduction / Catherine B. Lawrence and Gregory S. Fraley.
  • 2013 Springer
    Castellano, Immacolata; Merlino, Antonello.
    Gamma-Glutamyl Transpeptidases (g-GTs) are members of the N-terminal nucleophile hydrolase superfamily, enzymes that cleave the g-glutamyl amide bond of glutathione to liberate cysteinylglycine. The released g-glutamyl group can be transferred to water (hydrolysis) or to amino acids or short peptides (transpeptidation). g-GT plays a key role in the gamma glutamyl cycle by regulating the cellular levels of the antioxidant glutathione, hence it is a critical enzyme in maintaining cellular redox homeostasis.g-GT is upregulated during inflammation and in several human tumors, and it is involved in many physiological disorders related to oxidative stress, such as Parkinson's disease and diabetes. Furthermore, this enzyme is used as a marker of liver disease and cancer. This book covers current knowledge about the structure-function relationship of g-GTs and gives information about applications of g-GTs in different fields ranging from clinical biochemistry to biotechnology and biomedicine.
  • 2005 Springer
    Winterhager, Elke.
  • 2013 Springer
    Seidler, Norbert W.
    Also available: Print – 2013
  • 2011 Springer Protocols
    Gevaert, Kris; Vandekerckhove, Joël.
    Mass spectrometry-driven proteomics : an introduction / Kenny Helsens [and others] -- Metabolic labeling of model organisms using heavy nitrogen (15N) / Joost W. Gouw, Bastiaan B.J. Tops, and Jeroen Krijgsveld -- Trypsin-catalyzed oxygen-18 labeling for quantitative proteomics / Wei-Jun Qian [and others] -- ICPL labeling strategies for proteome research / Friedrich Lottspeich and Josef Kellermann -- Quantitative proteome analysis using isobaric peptide termini labeling (IPTL) / Magnus Ø. Arntzen [and others] -- Complete chemical modification of amine and acid functional groups of peptides and small proteins / Casey J. Krusemark [and others] -- Production and use of stable isotope-labeled proteins for absolute quantitative proteomics / Dorothée Lebert [and others] -- Organelle proteomics / Sophie Duclos and Michel Desjardins -- Membrane protein digestion -- comparison of LPI HexaLane with traditional techniques / Ping Sui [and others] -- GeLCMS for in-depth protein characterization and advanced analysis of proteomes / Alicia Lundby and Jesper V. Olsen -- Exploring new proteome space : combining Lys-N proteolytic digestion and strong cation exchange (SCX) separation in peptide-centric MS-driven proteomics / Nadia Taouatas, Shabaz Mohammed, and Albert J.R. Heck -- Quantitation of newly synthesized proteins by pulse labeling with azidohomoalanine / Gertjan Kramer [and others] -- Analytical strategies in mass spectrometry-based phosphoproteomics / Heidi Rosenqvist, Juanying Ye, and Ole N. Jensen -- Protocol on the use of titanium dioxide chromatography for phosphoproteomics / Martijn W.H. Pinkse, Simone Lemeer, and Albert J.R. Heck -- Positional proteomics at the N-Terminus as a means of proteome simplification / Gemma R. Davidson, Stuart D. Armstrong, and Robert J. Beynon -- N-Terminomics : a high-content screen for protease substrates and their cleavage sites / John C. Timmer and Guy S. Salvesen -- Protease specificity profiling by tandem mass spectrometry using proteome-derived peptide libraries / Oliver Schilling, Ulrich auf dem Keller, and Christopher M. Overall -- Identification of proteolytic products and natural protein N-Termini by terminal amine isotopic labeling of substrates (TAILS) / Alain Doucet [and others] -- Lectins as tools to select for glycosylated proteins / Els J.M. Van Damme -- Strong cation exchange chromatography for analysis of sialylated glycopeptides / Katharina Lohrig, Albert Sickmann, and Urs Lewandrowski -- Titanium dioxide enrichment of sialic acid-containing glycopeptides / Giuseppe Palmisano, Sara E. Lendal, and Martin R. Larsen -- Chemical De-O-glycosylation of glycoproteins for applications in LC-based proteomics / Franz-Georg Hanisch -- Ubiquitination and degradation of proteins / Yelena Kravtsova-Ivantsiv and Aaron Ciechanover -- Bioinformatics challenges in mass spectrometry-driven proteomics / Lennart Martens -- Case study on the comparison of different software tools for automated quantification of peptides / Niklaas Colaert [and others].
  • 2012 ScienceDirect
    Friedmann, Theodore.
    General principles of retrovirus vector design / Tammy Chang and Jiing-Kuan Yee -- Strategies for retrovirus-based correction of severe, combined immunodeficiency (SCID) / Alain Fischer, Salima Hacein-Bey-Abina, and Marina Cavazzana-Calvo -- Retrovirus and lentivirus vector design and methods of cell conditioning / Samantha Cooray, Steven J. Howe, and Adrian J. Thrasher -- Analysis of the clonal repertoire of gene-corrected cells in gene therapy / Anna Paruzynski ... [et al.] -- Developing novel lentiviral vectors into clinical products / Anna Leath and Kenneth Cornetta -- Lentivirus vectors in [beta]-thalassemia / Emmanuel Payen ... [et al.] -- Gene therapy for chronic granulomatous disease / Elizabeth M. Kang and Harry L. Malech -- Alternative splicing caused by lentiviral integration in the human genome / Arianna Moiani and Fulvio Mavilio -- Genotoxicity assay for gene therapy vectors in tumor prone mice / Eugenio Montini and Daniela Cesana -- Lentiviral hematopoietic cell gene therapy for X-linked adrenoleukodystrophy / Nathalie Cartier ... [et al.] -- Retroviral replicating vectors in cancer / Christopher R. Logg ... [et al.] -- Adeno-associated virus vectorology, manufacturing, and clinical applications / Joshua C. Grieger and R. Jude Samulski -- Gene delivery to the retina : from mouse to man / Jean Bennett, Daniel C. Chung, and Albert Maguire -- Generation of hairpin-based RNAi vectors for biological and therapeutic application / Ryan L. Boudreau and Beverly L. Davidson -- Recombinant adeno-associated viral vector reference standards / Philippe Moullier and Richard O. Snyder -- NIH oversight of human gene transfer research involving retroviral, lentiviral, and adeno-associated virus vectors and the role of the NIH Recombinant DNA Advisory Committee / Marina O'Reilly ... [et al.] -- Regulatory structures for gene therapy medicinal products in the European Union / Bettina Klug ... [et al.].
  • 2007 Springer
    Lankenau, Dirk-Henner.
    From microorganisms to humans, this volume provides an interdisciplinary overview of how genome integrity is maintained. It begins with DNA replication and continues with replicative DNA repair and pleiotropic protein interactions. It also reviews the cellular responses to radiation and genotoxic stress affecting whole genomes.
  • 2010 Springer
    Nasheuer, Heinz Peter.
    Coming full circle: Cyclin-dependent kinases as anti-cancer drug targets / R.P. Fisher -- Core and linker histone modifications involved in the DNA damage response / J.E. Chubb and S. Rea -- Chromatin assembly and signalling the end of DNA repair requires acetylation of histone H3 on lysine 56 / T. Costelloe and N.F. Lowndes -- Structure and function of histone H2AX / D.M. Pinto and A. Flaus -- The initiation step of eukaryotic DNA replication / H. Pospiech, F. Grosse and F.M. Pisani -- Non-coding RNAs: New players in the field of eukaryotic DNA replication / T. Krude -- Function of TopBP1 in Genome Stability / M. Sokka ... [et al.] -- Eukaryotic single-stranded DNA binding proteins: central factors in genome stability / S. Broderick ... [et al.] -- DNA polymerases and mutagenesis in human cancers / E. Crespan, A. Amoroso and G. Maga -- DNA polymerase [eta], a key protein in translesion synthesis in human cells / S. Cruet-Hennequart ... [et al.] -- The mitochondrial DNA polymerase in health and disease / W.C. Copeland -- Centromeres: assembling and propagating epigenetic function / M. Glynn ... [et al.] -- Nucleotide excision repair in higher eukaryotes: mechanism of primary damage recognition in global genome repair / N.I. Rechkunova and O.I. Lavrik -- Nonhomologous DNA end joining (NHEJ) and chromosomal translocations in humans / M.R. Lieber ... [et al.] -- Fluorescence-based quantification of pathway-specific DNA double-strand break repair activities: a powerful method for the analysis of genome destabilizing mechanisms / M. Bohringer and L. Wiesmuller -- Apoptosis: a way to maintain healthy individuals / C. Mondello and A.I. Scovassi -- The use of transgenic mice in cancer and genome stability research / S. Conmy and H.P. Nasheuer.
  • 2011
    Airen, Isoken Omosefe; Covert, Markus; Spormann, Alfred M.; Swartz, James R.
    We set out to develop and apply a high-throughput cell-free protein synthesis (CFPS) platform that provides functional genomics information for a wide variety of open reading frames (ORFs). We then used this information to improve CFPS yields by 4- to 5-fold, depending on the protein product. With the increasing number of completed genome sequences and ongoing sequencing projects, the post-genomic era has ushered in the promise of complete understanding of biological systems. For such a task, the most important set of information is inarguably the knowledge of the function of each gene product. To lead this endeavor of discerning the properties and functions of the entirety of an organism's genes and gene products, the field of functional genomics has emerged. Functional genomics focuses on dynamic cellular aspects, such as gene transcription, translation, and protein-protein interactions, in attempts to understand the relationship between an organism's genome and its phenotype. Thus, the ultimate goal of such studies is to provide a more complete picture of how biological function arises from the hereditary information of a living system. However, despite this clear interest in analyzing the expression and function of gene products, the development of techniques to address the high-throughput needs of functional genomics has been challenging, given the large diversity of protein functions and physiochemical properties, such as molecular weight and hydrophobicity, as well as the varying expression levels of proteins within a cell. In light of these challenges, we developed a sequential CFPS platform, which is capable of characterizing a variety of diverse proteins in the context of the dynamic metabolic networks that exist in vivo. The first round of expression is directed by PCR-generated expression templates (ETs) and creates an array of cell extracts that are individually enriched with a single target gene product. This round of CFPS is terminated by the selective degradation of the linear DNA templates, and a subsequent round of protein expression is initiated by the addition of a plasmid ET for a reporter protein. The array is then screened to identify gene products that enhanced or inhibited the expression and folding of the reporter. With such a method, we expect that the observations will expand our knowledge of both cell-free and in vivo metabolism, as well as identify key factors and reactions that could potentially lead to improved in vitro transcription, translation and protein folding. CFPS systems offer attractive alternatives to conventional fermentation processes used for protein production. Although improvements in CFPS energetics and reaction conditions have greatly enhanced in vitro protein synthesis, we believe that there are still other issues limiting the productivity of the technology. For this reason, identifying targets that could further improve CFPS is desirable. To validate the developed sequential CFPS protocol, we conducted a genome-wide survey of the well-studied bacterium Escherichia coli (E. coli) to identify soluble gene products that influence the in vitro metabolism. With this method, we identified 139 gene products (79 positive and 60 negative effectors) that influenced the cell-free transcription, translation, and protein folding of our three reporter proteins, as well as the energy metabolism and RNA and protein stability in the CFPS system. Encouragingly, most of the observed effects were consistent with the accepted in vivo metabolic functions of the gene products. However, many were not and required subsequent assays and in-depth literature searches to suggest hypotheses for the in vitro activities of the identified gene products. In many cases, the observations illuminated principles and influences that are unknown, lesser known, or secondary functions that were not expected to influence the CFPS performance. The information from the genome-wide survey was then used to guide modifications of the CFPS system to improve the productivity and duration of in vitro protein synthesis, as well as the efficiency of protein folding. First, fifteen positive effectors were produced and supplemented into the expression reactions in various combinations of the effectors in order to identify cooperative interactions that further enhance system performance. Next, we constructed and evaluated four mutant E. coli strains with chromosomal deletions in non-essential genes that encode negative effectors identified by the genomic survey. We also re-optimized the small molecule metabolite environment in the CFPS reactions. Thus, in the improved in vitro expression system, energy generation, translation initiation and elongation, and protein folding were enhanced; the reaction pH was stabilized; the supplies of specific molecular substrates that are essential for protein synthesis were replenished; and mRNA transcripts were stabilized. With this new system, the total, soluble, and active yields of the several diverse proteins were enhanced by 300 to 400%. The functional genomic analysis of E. coli has greatly broadened our understanding of the biology of the organism. And with the use of species-independent translational leaders that can facilitate cell-free expression (Mureev et al., 2009), our sequential CFPS platform can be used for similar genome-wide surveys of most organisms. In this way, the vast wealth of information available in the sequenced genomes will be utilized, and our knowledge of these biological systems will be significantly improved. Furthermore, the forward, or targeted, metabolic engineering strategy that was used to enhance our CFPS system can be applied to the development and/or improvement of most organism-based in vitro protein expression platforms. These targeted metabolic changes will lead to more rapid and more significant enhancements than traditional improvement strategies, as well as bring us closer to a complete understanding of the biological systems.
  • 2010
    Paulsen, Renee Darlene; Cimprich, Karlene; Meyer, Tobias; Wang, Clifford; Wysocka, Joanna.
    Genome instability has long been known to be a hallmark of cancerous cells, but the cellular causes and consequences of such instability are still not fully understood. Mutations, translocations, DNA rearrangements, as well as chromosomal loss can all result in the loss of genomic integrity. To prevent the disruption of cellular homeostasis due to DNA damage accumulation, cells contain pathways to sense and respond to DNA damage including cell cycle checkpoints and numerous DNA repair processes, collectively known as the DNA damage response (DDR). Mutations in many of the genes involved in the DDR are linked to several diseases, including premature aging, neurodegeneration and cancer. These signaling pathways are especially critical during DNA replication when the DNA is unwound and vulnerable to processing. Here, the cell relies on the S-phase checkpoint to sense DNA damage at the sites of replication forks and to facilitate a number of downstream pathways to maintain genomic stability. These processes include blocking further origin firing, facilitating DNA repair, preventing cell cycle progression, and stabilizing stalled replication forks. Here, two genome-wide siRNA screens were employed to identify additional genes involved in genome stabilization by monitoring phosphorylation of the histone variant H2AX, an early mark of DNA damage. The first screen looked at H2AX phosphorylation that occurred simply by individual protein depletion, and the second screen used a low level of a replication inhibitor, aphidicolin, to specifically identify genes that were needed to prevent DNA damage during S-phase, potentially due to the loss of replication fork stabilization mechanisms. While the results from the second screen are still undergoing further characterization, we did discover hundreds of genes whose down-regulation led to elevated levels of H2AX phosphorylation in the absence of any external stress. From this gene set, we identified many gene networks that were significantly enriched amongst our screening hits as well as several intriguing individual genes that were chosen for follow up study. These included genes involved in mRNA processing, the pathology of Charcot-Marie-Tooth syndrome, and the histone methyl transferase protein, Set8.
  • 2014 Springer
    Marko-Varga, György A.
    This book offers a valuable resource that allows students, researchers, educators and the general public to learn about proteomics and genomics. Chromosomes form the basis for our genetic heritage and are the code for protein synthesis. The Human Genome Map was presented in 2002, and the Proteome Sequence Map is currently being created by a global consortia initiative. Proteome and genome building blocks already form the basis of scientific research areas and shape major areas of the pharmaceutical and biomedical industries. The book provides background information on and our current understanding of these gene and protein areas, and explains in detail how cutting-edge science is using these resources to develop new medicines and new diagnostics for patient treatment and care. The book will benefit all students and researchers who need a good understanding of genomics and proteomics within the clinical field. Its content is also suitable for a broad readership, including those not specialized in this field. Dr. Marko-Varga is head of Div. Clinical Protein Science & Imaging at the Biomedical Center, Dept. of Measurement Technology and Industrial Electrical Engineering, Lund University. He's also Professor at the 1st Department of Surgery, Tokyo Medical University, Tokyo, Japan.
  • 2012 ScienceDirect
    Kangawa, Kenji; Kojima, Masayasu.
    History to the discovery of ghrelin / Cyril Y. Bowers -- Orphan GPCRs and methods for identifying their ligands / Morikatsu Yoshida, Mikiya Miyazato, and Kenji Kangawa -- Purification of rat and human ghrelins / Masayasu Kojima, Hiroshi Hosoda, and Kenji Kangawa -- Variety of acyl modifications in mammalian ghrelins / Takanori Ida -- Determination of nonmammalian ghrelin / Hiroyuki Kaiya ... [et al.] -- Morphological analysis of ghrelin neurons in the hypothalamus / Haruaki Kageyama, Fumiko Takenoya, and Seiji Shioda -- High-performance liquid chromatography analysis of hypothalamic ghrelin / Takahiro Sato and Masayasu Kojima -- Standard sample collections for blood ghrelin measurements / Hiroshi Hosoda and Kenji Kangawa -- From ghrelin to ghrelin's O-acyl transferase / Jesus A. Gutierrez ... [et al.] -- Enzymatic characterization of GOAT, ghrelin O-acyltransferase / Hideko Ohgusu, Tomoko Takahashi, and Masayasu Kojima -- The study of ghrelin deacylation enzymes / Motoyasu Satou and Hiroyuki Sugimoto -- Synthesis of ghrelin : chemical synthesis and semisynthesis for large-scale preparation of modified peptides / Tomohiro Makino, Masaru Matsumoto, and Yoshiharu Minamitake -- Ghrelin O-acyltransferase assays and inhibition / Martin S. Taylor ... [et al.] -- Model-based evaluation of growth hormone secretion / Johannes D. Veldhuis and Daniel M. Keenan -- Ghrelin in the control of energy, lipid, and glucose metabolism / Kristy M. Heppner ... [et al.] -- Ghrelin and the vagus nerve / Yukari Date -- Measurement of AMP-activated protein kinase activity and expression in response to ghrelin / Chung Thong ... [et al.] -- Ghrelin and gastrointestinal movement / Naoki Fujitsuka ... [et al.] -- Ghrelin acylation by ingestion of medium-chain fatty acids / Yoshihiro Nishi, Hiroharu Mifune, and Masayasu Kojima -- Islet [beta]-cell ghrelin signaling for inhibition of insulin secretion / Katsuya Dezaki and Toshihiko Yada -- Rikkunshito as a ghrelin enhancer / Hiroshi Takeda ... [et al.] -- Thermogenic characterization of ghrelin receptor null mice / Ligen Lin and Yuxiang Sun -- Transgenic mice overexpressing ghrelin or ghrelin analog / Hiroyuki Ariyasu ... [et al.] -- Therapeutic potential of ghrelin in restricting-type anorexia nervosa / Mari Hotta ... [et al.] -- Clinical application of ghrelin for chronic respiratory diseases / Nobuhiro Matsumoto and Masamitsu Nakazato -- Clinical trial of ghrelin synthesis administration for upper GI surgery / Shuji Takiguchi ... [et al.].
  • 2013 Springer
    Barańska, Jolanta.
    Also available: Print – 2013
  • pt. A-B, 2008. ScienceDirect
    pt. B, 2008 ScienceDirect
    Poole, Robert K.
    Also available: Print – pt. A-B, 2008.
  • Glick, David M.
  • 2015 Springer
    Harris, Charles; Wang, Jen-Chywan.
    This timely volume provides a comprehensive overview of glucocorticoids and their role in regulating many aspects of physiology and their use in the treatment of disease. The book is broken into four sections that begin by giving a general introduction to glucocorticoids and a brief history of the field. The second section will discuss the effects of glucocorticoids on metabolism, while the third section will cover the effects of glucocorticoids on key tissues. The final section will discuss general topics, such as animal models in glucocorticoid research and clinical implications of glucocorticoid research. Featuring chapters from leaders in the field, this volume will be of interest to both researchers and clinicians.
    Also available: Print – 2015
  • 2005 ScienceDirect
    Packer, Lester; Sies, H.
    Also available: Print – 2005
  • 2011 Springer
    Pavão, Mauro S. G.
    Heparan sulfate proteoglycan in inflammation and angiogenesis / Lianchun Wang -- Heparan sulfate proteoglycans in infection / Allison H. Bartlett and Pyong Woo Park -- Glycans in cancer / Lubor Borsig -- Glycosaminoglycans in atherosclerosis and thrombosis / C.P. Vicente, J.A.P. Godoy, and C.C. Werneck -- Glycans in embryonic stem cells / Claire Pickford ... [et al.] -- Heparan sulfate proteoglycans in cancer therapy / Ezeddin Salem Gassar, Sherif A. Ibrahim, and Martin Götte -- Structure and biological activities of glycosaminoglycan analogs from marine invertebrates : new therapeutic agents? / Eliene O. Kozlowski ... [et al.]
    Also available: Print – 2011
  • 2010 ScienceDirect
    Fukuda, Minoru.
    Also available: Print – 2010
  • 2005 Springer
    Axford, John S.
    Also available: Print – 2005
  • 2006 Springer Protocols
    Brockhausen, Inka.
    Gene expression analysis of glycosylation-related genes by real-time polymerase chain reaction / Juan J. Garcia-Vallejo ... [et al.] -- Analysis of the glycodynamics of primary osteoblasts and bone cancer cells / Inka Brockhausen-Schutzbach, Xiaojing Yang, and Mark Harrison -- Micromethods for the characterization of lipid a core and o-antigen lipopolysaccharide / Cristina L. Marolda ... [et al.] -- Assays for a galactosyltransferase involved in the assembly of the O7 antigen repeat unit of escherichia coli / Inka Brockhausen-Schutzbach ... [et al.] -- High-throughput quantitation of metabolically labeled anionic glycoconjugates by scintillation proximity assay utilizing binding to cationic dyes / Karen J. Rees-Milton and Tassos P. Anastassiades -- Quantitative analysis of mucins in mucosal secretions using indirect ELISA / Pablo Argueso and Ilene K. Gipson -- Molecular dissection of the mouse zona pellucida: an electron microscopic perspective utilizing high-resolution colloidal-gold labeling methods / Frederick W. K. Kan -- Soluble adamantly glycosphingolipid analogs as probes of glycosphingolipid function / Clifford Lingwood ... [et al.] -- Diagnosis of Krabbe disease by use of a natural substrate / John W. Callahan and Marie-Anne Skomorowski -- In vitro assays of the functions of calnexin and calreticulin, lectin-chaperones of the endoplasmic reticulum / Breanna S. Ireland, Monika Niggemann, and David B. Williams -- Quantitative measurement of selectin-ligand interactions: assays to identify a sweet pill in a library of carbohydrates / Mark E. Beauharnois, Sriram Neelamegham, and Khushi L. Matta -- Sialic acid-binding specificities of siglecs / Nadine Bock and Soerge Kelm. The application of fluorophore-assisted carbohydrate -- Electrophoresis for the study of the dolichol pyrophosphate-linked oligosaccharides pathway in cell cultures and animal tissues / Ningguo Gao -- Partial purification of mannosylphosphorylundecaprenol synthase from micrococcus luteus: a useful enzyme for the biosynthesis of a variety of mannosylphosphorylpolyisoprenol products / Jeffrey S. Rush and Charles J. Waechter -- Assay of dolichyl-phospho-mannose synthase reconstituted in a lipid matrix / John S. Schutzbach -- O-mannosylation in mammalian cells / Tamao Endo and Hiroshi Manya -- Methods for analysis of unusual forms of o-glycosylation / Aleksandra Nita-Lazar and Robert S. Haltiwanger -- Structural analysis of naturally occurring sialic acids / Johannis P. Kamerling and Gerrit J. Gerwig -- Non-radioactive trans-sialidase screening assay / Silke Schrader and Roland Schauer -- Structural determination of o-glycans by tandem mass spectrometry / Catherine Robbe, Jean-Claude Michalski, and Calliope Capon -- Detailed structural analysis of n-glycans released from glycoproteins in SDS-page gel bands using HPLC combined with exoglycosidase array digestions / Louise Royle ... [et al.] -- Molecular modeling of glycosyltransferases / Anne Imberty ... [et al.] -- Galactoside_2,6-sialyltransferase (st6gal.i) and the sialyl_2,6-galactosyl-linkage in tissues and cell lines / Fabio Dall'Olio, Nadia Malagolini, and Mariella Chiricolo -- Visualizing intracellular distribution and activity of core 2 _(1,6)n-acetylglucosaminyltransferase-I (C2GNT-I) in living cells / Maelle Prorok-Hamon, Sylvie Mathieu, and Assou El-Battari --
  • 2015 Springer Protocols
    Castilho, Alexandra.
    Current approaches to engineering N-linked protein glycosylation in bacteria / Andreas Naegeli and Markus Aebi -- Inverse metabolic engineering for enhanced glycoprotein production in Escherichia coli / Stephen R. P. Jaffé, Benjamin Strutton, Jagroop Pandhal, and Phillip C. Wright -- GlycoSNAP : a high-throughput screening methodology for engineering designer glycosylation enzymes / Anne A. Ollis, Yi Chai, and Matthew P. DeLisa -- Production of glycoproteins with asparagine-linked N-acetylglucosamine in Escherichia coli / Flavio Schwarz and Markus Aebi -- Glyco-engineering O-antigen-based vaccines and eiagnostics in E. coli / Nicolas F. Vozza and Mario F. Feldman -- Progress in yeast glycosylation engineering / Stephen R. Hamilton and Dongxing Zha -- Protein production with a Pichia pastoris OCH1 knockout strain in fed-batch mode / Christoph Gmeiner and Oliver Spadiut -- Engineering the Pichia pastoris N-glycosylation pathway using the GlycoSwitch technology / Bram Laukens, Charlot De Wachter, and Nico Callewaert -- Development of a valuable yeast strain using a novel mutagenesis technique for the effective production of therapeutic glycoproteins / Hiroko Abe and Ken-ichi Nakayama -- An overview and history of glyco-engineering in insect expression systems / Christoph Geisler, Hideaki Mabashi-Asazuma, and Donald L. Jarvis 11 SweetBac : applying multiBac technology towards flexible modification of insect cell glycosylation / Dieter Palmberger and Dubravko Rendic -- Engineering sialic acid synthesis ability in insect cells / Karthik Viswanathan, Someet Narang, and Michael J. Betenbaugh -- Engineering N-glycosylation pathway in insect cells : suppression of [beta]-N-acetylglucosaminidase and expression of [beta]-1,4-galactosyltransferase / Yeon Kyu Kim and Hyung Joon Cha -- N-glyco-engineering in plants : update on strategies and major achievements / Herta Steinkellner and Alexandra Castilho -- Gene targeting for precision glyco-engineering : production of biopharmaceuticals devoid of plant-typical glycosylation in moss bioreactors / Eva L. Decker, Gertrud Wiedemann, and Ralf Reski -- Im"plant"ing of mammalian glycosyltransferase gene into plant suspension-cultured cells using agrobacterium-mediated transformation / Hiroyuki Kajiura and Kazuhito Fujiyama -- Transient glyco-engineering of N. benthamiana aiming at the synthesis of multi-antennary sialylated proteins / Andreas Loos and Alexandra Castilho -- Subcellular targeting of proteins involved in modification of plant N- and O-glycosylation / Martina Dicker, Jennifer Schoberer, Ulrike Vavra, and Richard Strasser -- Assembly of multigene constructs using golden gate cloning / Sylvestre Marillonnet and Stefan Werner -- Strategies for engineering protein N-glycosylation pathways in mammalian cells / Qiong Wang, Mark Stuczynski, Yuan Gao, and Michael J. Betenbaugh -- Glycan remodeling with processing inhibitors and lectin- resistant eukaryotic cells / Veronica T. Chang, Robert A. Spooner, Max Crispin, and Simon J. Davis -- Production of highly sialylated recombinant glycoproteins using Ricinus communis agglutinin-I-resistant CHO glycosylation mutants / John S. Y. Goh, Kah Fai Chan, and Zhiwei Song -- Metabolic glyco-engineering in eukaryotic cells and selected applications / Friedrich Piller, Aline Mongis, and Véronique Piller -- Evaluation of quenching and extraction methods for nucleotide/nucleotide sugar analysis / Katrin Braasch, Carina Villacrés, and Michael Butler -- Chemoenzymatic glyco-engineering of monoclonal antibodies / John P. Giddens and Lai-Xi Wang -- Chemical polysialylation of recombinant human proteins / Ivan V. Smirnov, Ivan I. Vorobiev, Alexey A. Belogurov, Dmitry D. Genkin, Sergey M. Deyev, and Alexander G. Gabibov -- Site-specific glycosylation profiling using liquid chromatography-tandem mass spectrometry (LC-MS) / Clemens Gruber and Friedrich Altmann -- Mass spectrometric analysis of oligo- and polysialic acids / Christina E. Galuska, Kai Maass, and Sebastian P. Galuska -- Isomer-specific analysis of released N-glycans by LC-ESI MS/MS with porous graphitized carbon / Daniel Kolarich, Markus Windwarder, Kathirvel Alagesan, and Friedrich Altmann.
  • 2015 Springer Protocols
    Frank, Martin; Lütteke, Thomas.
    Annotation of glycomics MS and MS/MS spectra using the glycoworkbench software tool -- Glycobase and autogGU : resources for interpreting HPLC-glycan data -- NMR chemical shift prediction of glycans : application of the computer program CASPER in structural analysis -- Handling and conversion of carbohydrate sequence formats and monosaccharide notation -- Bacterial, plant, and fungal carbohydrate structure databases : daily usage -- Using NMR data on GLYCOSCIENCES.de -- Glycomic analysis using KEGG GLYCAN -- GlycomeDB -- Eukaryotic glycosylation : online methods for site prediction on protein sequences -- Analyzing glycan structure synthesis with the glycan pathway predictor (GPP) tool -- Functional network in posttranslational modifications : Glyco-Net in Glycoconjugate Data Bank -- JCGGDB : Japan Consortium for Glycobiology and Glycotechnology Database -- Glycan array data management at Consortium for Functional Glycomics -- Analyzing glycan-binding patterns with the profilepSTMM tool -- Exploring the specificities of glycan-binding proteins using glycan array data and the GlycoSearch software -- Statistical analysis of amino acids in the vicinity of carbohydrate residues performed by GlyVicinity -- Tools to assist determination and validation of carbohydrate 3D structure data -- Glyco3D : a portal for structural glycosciences -- Solution conformation of carbohydrates : a view by using NMR assisted by modeling -- Informing saccharide structural NMR studies with density functional theory calculations -- Energy maps for glycosidic linkage conformations -- Conformational analysis of oligosaccharides and polysaccharides using molecular dynamics simulations -- Molecular dynamics simulations of membrane- and protein-bound glycolipids using GLYCAM -- Lipopolysaccharide membrane building and simulation -- Molecular dynamics simulations of glycoproteins using CHARMM -- Calculating binding free energies for protein-carbohydrate complexes -- Scoring functions for AutoDock -- Structures of glycans bound to receptors from saturation transfer difference (STD) NMR spectroscopy : quantitative analysis by using CORCEMA-ST -- QM/MM methods for studying enzymatic reactions of glycosyltransferases.
  • 2010 ScienceDirect
    Fukuda, Minoru.
    Also available: Print – 2010
  • 2009 Springer Protocols
    Karlsson, Niclas G.; Packer, Nicolle.
    Analysis of N- and O-linked glycans from glycoproteins using MALTI-TOF mass spectrometry / Willy Morelle ... [et al.] -- Infrared multiphoton dissociation mass spectrometry for structural elucidation of oligosaccharides / Bensheng Li ... [et al.] -- Mass spectrometry of N-linked glycans / Parastoo Azadi and Christian Heiss -- Solid-phase permethylation for glycomic analysis / Yehia Mechref, Pilsoo Kang, and Milos V. Novotny -- Method for investigation of oligosaccharides using phenylhydrazine derivatization / Erika Lattová and Hélène Perreault -- Two-dimensional HPLC separation with reverse-phase-nano-LC-MS/MS for the characterization of glycan pools after labeling with 2-aminobenzamide / Manfred Wuhrer, Carolien A.M. Koeleman, and André M. Deelder -- Capillary lectin-affinity electrophoresis for glycan analysis / Kazuaki Kakehi and Mitsuhiro Kinoshita -- Analysis of methylated O-glycan alditols by reversed-phase nanoLC coupled CAD-ESI mass spectrometry / Franz-Georg Hanisch and Stefan Müller -- High-throughput and high-sensitivity nano-LC/MS and MS/MS for O-glycan profiling / Hasse Karlsson ... [et al.] -- Collision-induced dissociation tandem mass spectrometry for structural elucidation of glycans / Bensheng Li ... [et al.] -- The structural elucidation of glycosaminoglycans / Vikas Prabhakar, Ishan Capila, and Ram Sasisekharan -- Labelling heparan sulphate saccharides with chromophore fluorescence and mass tags for HLC and MS separations / Mark Skidmore ... [et al.] -- Small-scale enzymatic digestion of glycoproteins and proteoglycans for analysis of oligosaccharides by LC-MS and FACE gel electrophoresis / Ruby P. Estrella ... [et al.] -- Enrichment strategies for glycopeptides / Shigeyasu Ito, Ko Hayama, and Jun Hirabayashi -- Introductory glycosylation analysis using SDS-PAGE and peptide mass fingerprinting / Nicole Wilson, Raina Simpson, and Catherine Cooper-Liddell -- Characterization of N-linked glycosylation on recombinant glycoproteins produced in Pichia pastoris using ESI-MS and MALDI-TOF / Bing Gong ... [et al.] -- N-glycosylation site analysis of human platelet proteins by hydrazide affinity capturing and LC-MS/MS / Urs Lewandrowski and Albert Sickmann -- LC/MSn for glycoprotein analysis : N-linked glycosylation analysis and peptide sequencing of glycopeptides / Nana Kawasaki, Satsuki Itoh, and Teruhide Yamaguchi -- Detecting the "O-GlcNAcome" : detection, purification, and analysis of O-GlcNAc modified proteins / Natasha E. Zachara -- Preparation of a glycan library using a variety of glycosyltrasferases [i.e. glycosyltransferases] / Hiromi Ito ... [et al.] -- Data mining the PDB for glyco-related data / Thomas Lütteke and Claus-W. von der Lieth -- Saccharide microarrays for high-throughput interrogation of glycan-protein binding interactions / Andrew K. Powell, Zheng-liang Zhi, and Jeremy E. Turnbull -- Glocosaminoglycan characterization methodologies probing biomolecular interactions / Vikas Prabhakar, Ishan Capila, and Ram Sasisekharan -- Development and characterization of antibodies to carbohydrate antigens / Jamie Heimburg-Molinaro and Kate Rittenhouse-Olson -- Inhibition of glcosyltransferase activities as the basis for drug development / John Schutzbach and Inka Brockhausen -- Saturation transfer difference NMR spectroscopy as a technique to investigate protein-carbohydrate interactions in solution / Thomas Haselhorst, Anne-Christin Lamerz, and Mark von Izstein.
  • 2015 Springer Protocols
    Balagurunathan, Kuberan; Desai, Umesh R.; Nakato, Hiroshi.
    Automated Synthesis of Chondroitin Sulfate Oligosaccharides / Chien-Fu Liang, Heung Sik Hahm, and Peter H. Seeberger -- Enzymatic Synthesis of Heparan Sulfate and Heparin / April Joice [and six others] -- Production of Size-Defined Heparosan, Heparan Sulfate, and Heparin Oligosaccharides by Enzymatic Depolymerization / Spencer Brown and Balagurunathan Kuberan -- Chemical Modification of Heparin and Heparosan / Karthik Raman, Balagurunathan Kuberan, and Sailaja Arungundram -- Synthesis of Sulfur Isotope-Labeled Sulfate Donor, 3'-Phosphoadenosine-5'- Phosphosulfate, for Studying Glycosaminoglycan Functions/ Caitlin Mencio [and three others] -- Preparation of Isotope-Enriched Heparan Sulfate Precursors for Structural Biology Studies / Xylophone V. Victor [and three others] -- Synthesis of Glycosaminoglycan Mimetics Through Sulfation of Polyphenols / Rami A. Al-Horani [and three others] -- Synthesis of Selective Inhibitors of Heparan Sulfate and Chondroitin Sulfate Proteoglycan Biosynthesis / Caitlin Mencio [and three others] -- Ascidian (Chordata-Tunicata) Glycosaminoglycans : Extraction, Purification, Biochemical, and Spectroscopic Analysis / Mauro S.G. Pavão -- Human Blood Glycosaminoglycans : Isolation and Analysis / Md. Ferdous Anower-E-Khuda and Koji Kimata -- Chromatographic Molecular Weight Measurements for Heparin, Its Fragments and Fractions, and Other Glycosaminoglycans / Barbara Mulloy and John Hogwood -- Mass Spectrometric Methods for the Analysis of Heparin and Heparan Sulfate / Miroslaw Lech, Ishan Capila, and Ganesh V. Kaundinya -- Validated Capillary Electrophoretic Assays for Disaccharide Composition Analysis of Galactosaminoglycans in Biologic Samples and Drugs/Nutraceuticals / Athanasia P. Asimakopoulou [and five others] -- Fast Screening of Glycosaminoglycan Disaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) : Applications to Biologic Samples and Pharmaceutical Formulations / Evgenia Karousou [and seven others] -- Capillary Electrophoretic Analysis of Isolated Sulfated Polysaccharides to Characterize Pharmaceutical Products / Zachary Shriver and Ram Sasisekharan -- Methods for Measuring Exchangeable Protons in Glycosaminoglycans / Consuelo N. Beecher and Cynthia K. Larive -- Heparan Sulfate Structure : Methods to Study N-Sulfation and NDST Action / Anders Dagälv [and five others] -- Analysis of Hyaluronan Synthase Activity / Davide Vigetti [and three others] -- A Rapid, Nonradioactive Assay for Measuring Heparan Sulfate C-5 Epimerase Activity Using Hydrogen/Deuterium Exchange-Mass Spectrometry / Ponnusamy Babu [and three others] -- Aggrecan : Approaches to Study Biophysical and Biomechanical Properties / Hadi Tavakoli Nia, Christine Ortiz, and Alan Grodzinsky -- Use of Flow Cytometry for Characterization and Fractionation of Cell Populations Based on Their Expression of Heparan Sulfate Epitopes / Rebecca J. Holley [and five others] -- A Transgenic Approach to Live Imaging of Heparan Sulfate Modification Patterns / Matthew Attreed and Hannes E. Bülow -- Informatics Tools to Advance the Biology of Glycosaminoglycans and Proteoglycans / Lewis J. Frey -- Designing "High-Affinity, High-Specificity" Glycosaminoglycan Sequences Through Computerized Modeling / Nehru Viji Sankaranarayanan [and three others] -- Using Isothermal Titration Calorimetry to Determine Thermodynamic Parameters of Protein-Glycosaminoglycan Interactions/ Amit K. Dutta, Jörg Rösgen, and Krishna Rajarathnam -- Characterizing Protein-Glycosaminoglycan Interactions Using Solution NMR Spectroscopy / Prem Raj B. Joseph, Krishna Mohan Poluri, Krishna Mohan Sepuru, and Krishna Rajarathnam -- Glycosaminoglycan-Protein Interaction Studies Using Fluorescence Spectroscopy / Rio S. Boothello, Rami A. Al-Horani, and Umesh R. Desai -- Studying Glycosaminoglycan-Protein Interactions Using Capillary Electrophoresis / Aiye Liang and Umesh R. Desai -- Histochemical Analysis of Heparan Sulfate 3-O-Sulfotransferase Expression in Mouse Brain / Tomio Yabe and Nobuaki Maeda -- Keratan Sulfate : Biosynthesis, Structures, and Biological Functions / Kenji Uchimura -- The Sulfs : Expression, Purification, and Substrate Specificity / Kenji Uchimura -- The Detection of Glycosaminoglycans in Pancreatic Islets and Lymphoid Tissues / Marika Bogdani [and five others] -- Nonradioactive Glycosyltransferase and Sulfotransferase Assay to Study Glycosaminoglycan Biosynthesis / Cheryl M. Ethen [and five others] -- Mapping Proteoglycan Functions with Glycosidases / Mauricio Cortes, Leslie K. Cortes, and Nancy B. Schwartz -- Cell Substrate Patterning with Glycosaminoglycans to Study Their Biological Roles in the Central Nervous System / Tony W. Hsiao [and three others] -- Analyzing the Role of Heparan Sulfate Proteoglycans in Axon Guidance In Vivo in Zebrafish / Fabienne E. Poulain -- Murine Models in the Evaluation of Heparan Sulfate-Based Anticoagulants / David Gailani, Qiufang Cheng, and Ivan S. Ivanov -- Genetic Approaches in the Study of Heparan Sulfate Functions in Drosophila / Masahiko Takemura and Hiroshi Nakato -- Measuring Sulfatase Expression and Invasion in Glioblastoma / Anna Wade [and three others] -- Synthesis and Biomedical Applications of Xylosides / Mausam Kalita [and four others] -- A Strategic Approach to Identification of Selective Inhibitors of Cancer Stem Cells / Nirmita Patel, Somesh Baranwal, and Bhaumik B. Patel -- Analysis of the Heavy-Chain Modification and TSG-6 Activity in Pathological Hyaluronan Matrices / Mark E. Lauer [and three others] -- Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration / Hong Qiu [and three others] -- Glycosaminoglycan Functionalized Nanoparticles Exploit Glycosaminoglycan Functions / James A. Vassie, John M. Whitelock, and Megan S. Lord -- Role of Glycosaminoglycans in Infectious Disease / Akiko Jinno and Pyong Woo Park -- Isolation and Purification of Versican and Analysis of Versican Proteolysis / Simon J. Foulcer, Anthony J. Day, and Suneel S. Apte -- Analysis of Human Hyaluronan Synthase Gene Transcriptional Regulation and Downstream Hyaluronan Cell Surface Receptor Mobility in Myofibroblast Differentiation / Adam C. Midgley and Timothy Bowen.
  • 2015 ScienceDirect
    Ball, Lauren E.; Drake, Richard R.
  • 2013 Springer Protocols
    Brockhausen, Inka.
    Glycan microarray screening assay for glycosyltransferase specificities / W. Peng, C.M. Nycholat and N. Razi -- A fluorescence-based assay for core 1 [beta]3galactosyltransferase (T-synthase) activity / T. Ju and R.D. Cummings -- Structural and biochemical analysis of a bacterial glycosyltransferase / F. Zhu ... [et al.] -- Study of the biological functions of mucin type core 3 O-glycans / S.H. Lee and M. Fukuda -- Generation of anti-sulfated glycan antibodies using sulfotransferase-deficient mice / H. Kawashima -- Fluorescent microscopy as a tool to elucidate dysfunction and mislocalization of golgi glycosyltransferases in COG complex depleted mammalian cells / R.A. Willett, I.D. Pokrovskaya and V.V. Lupashin -- A practical approach to reconstruct evolutionary history of animal sialyltransferases and gain insights into the sequence-function relationships of golgi-glycosyltransferases / D. Petit ... [et al.] -- Fluorescent lectin staining of drosophila embryos and tissues to detect the spatial distribution of glycans during development / E. Tian, L. Zhang and K.G. Ten Hagen -- Photoaffinity labeling of protein O-mannosyltransferases of the PMT1/PMT2 subfamily / M. Loibl and S. Strahl -- Enzymatic analysis of the protein O-glycosyltransferase, rumi, acting toward epidermal growth factor-like (egf) repeats / H. Takeuchi and R.S. Haltiwanger -- Enzymatic characterization of recombinant enzymes of O-glcNAc cycling / E.J. Kim and J.A. Hanover -- Antibodies and activity measurements for the detection of o-glcnac transferase and assay of its substrate, UDP-glcNAc / T. Lefebvre ... [et al.] -- In vitro glycosylation assay for bacterial oligosaccharyltransferases / M.A. Musumeci, M.V. Ielmini and M.F. Feldman -- In vitro UDP-sugar:Undecaprenyl-phosphate sugar-1-phosphate transferase assay and product detection by thin layer chromatography / K.B. Patel and M.A. Valvano -- In vitro O-antigen ligase assay / X. Ruan and M.A. Valvano -- Functional identification of bacterial glucosyltransferase WbdN / Y. Gao, A. Vinnikova and I. Brockhausen -- Methods for the pasteurella glycosaminoglycan synthases: Enzymes that polymerize hyaluronan, chondroitin, or heparosan chains / P.L. Deangelis -- Methods for measuring class i membrane-bound hyaluronan synthase activity / P.H. Weigel, A.J. Padgett-McCue and B.A. Baggenstoss -- Creation and characterization of glycosyltransferase mutants of Trypanosoma brucei / L. Izquierdo, M.L. Guther and M.A. Ferguson -- Mannose-6-phosphate: A regulator of LLO destruction / N. Gao and M.A. Lehrman -- N-acetylglucosaminyltransferase (GnT) assays using fluorescent oligosaccharide acceptor substrates: GnT-III, IV, V, and IX (GnT-Vb) / S. Takamatsu ... [et al.] -- A method for determination of UDP-GlcNAc: GlcNAc[beta]1-6(GlcNAc[beta]1-2)man[alpha]1-r [GlcNAc to man] beta1-4n-acetylglucosaminyltransferase vi activity / T. Taguchi and N. Taniguchi -- In vitro assays of orphan glycosyltransferases and their application to identify notch xylosyltransferases / M.K. Sethi ... [et al.] -- In vitro folding of [beta]-1,4galactosyltransferase and polypeptide-[alpha]-N-acetylgalactosaminyltransferase from the inclusion bodies / B. Ramakrishnan and P.K. Qasba -- An assay for [alpha] 1,6-fucosyltransferase (FUT8) activity based on the HPLC separation of a reaction product with fluorescence detection / H. Ihara ... [et al.] -- Immunodetection of glycosyltransferases in gastrointestinal tissues / J. Gomes and C.A. Reis -- Glycosyltransferases in chemo-enzymatic synthesis of oligosaccharides / B. Tefsen and I. van Die -- Tumor targeting by a carbohydrate ligand-mimicking peptide / S. Hatakeyama ... [et al.] -- Glycoengineering of human cell lines using zinc finger nuclease gene targeting: Simple cells with homogeneous GalNAc O-glycosylation allow isolation of the O-glycoproteome by one-step lectin affinity chromatography / C. Steentoft, E.P. Bennett and H. Clausen -- Generation of monoclonal antibodies to native active human glycosyltransferases / M.B. Vester-Christensen ... [et al.].
  • 2009 Springer
    Morré, D. James; Mollenhauer, Hilton H.
  • 2012 Springer
    Dupré, Denis J.; Hébert, Terence E.; Jockers, Ralf.
    ER-bound steps in the biosynthesis of G protein-coupled receptors / Christian Nanoff and Michael Freissmuth -- Role of chaperones in G Protein coupled receptor signaling complex assembly / Denis J. Dupré, Maha M. Hammad, Patrick Holland and Jaime Wertman -- GPCR oligomerization: contribution to receptor biogenesis / Kathleen Van Craenenbroeck -- The functional size of GPCRs: monomers, dimers or tetramers? / Darlaine Pétrin and Terence E. Hébert -- Regulation of post-Golgi traffic of G protein-coupled receptors / Guangyu Wu -- Regulated GPCR trafficking to the plasma membrane: general issues and the CCR5 chemokine receptor example / Hamasseh Shirvani, Gabriel Gätà and Stefano Marullo -- Regulatory processes governing the cell surface expression of LH and FSH receptors / Deborah L. Segaloff -- Chaperone-mediated assembly of G protein complexes / Barry M. Willardson and Christopher M. Tracy -- Synthesis and assembly of G protein [beta][gamma] dimers: comparison of in vitro and in vivo studies / Jane Dingus and John D. Hildebrandt -- Preferential assembly of G-[alpha][beta][gamma] complexes directed by the [gamma] subunits / Janet D. Robishaw -- G Protein trafficking / Philip B. Wedegaertner -- Differential assembly of GPCR signaling complexes determines signaling specificity / Pascal Maurice, Abla Benleulmi-Chaachoua and Ralf Jockers -- GPCR and voltage-gated calcium channels (VGCC) signaling complexes / Christophe Altier -- Pharmacological chaperones correct misfolded GPCRs and rescue function: protein trafficking as a therapeutic target / Guadalupe Maya-Núñez, Alfredo Ulloa-Aguirre, Jo Ann Janovick and P. Michael Conn.
  • 2007 Springer
    Bourne, Henry R.; Horuk, Richard; Kuhnke, J.; Michel, H.
  • 2007
    PrintStatus: Not Checked OutLane Catalog Record
  • 2002 Springer Protocols
    Hicks, Barry W.
  • 2006 Wiley
    Chalfie, Martin; Kain, Steven.
    "Green fluorescent protein (GFP), a biological marker cloned from jellyfish, has emerged from virtual obscurity to the forefront of biomolecular research in just a few years. GFP and related fluorescent proteins (FPs) have been intensely studied - the subjects of hundreds of in vivo projects worldwide. These studies have resulted in new applications and published articles, emerging at a breathtaking rate. The success of FPs and their many uses promise to make them some of the most powerful tools in biotechnology, pharmaceutical science, and molecular and cellular biology."
    Also available: Print – 2006
  • 2005 Springer
    Chowen, Julie A.; Varela-Nieto, Isabel.
    Basic physiology of the growth hormone/insulin-like growth factor (IGF)-axis / Laura M. Frago and Julie A. Chowen -- Experimental models for understanding the role of insulin-like growth factor-I and its receptor during development / Jose Luis Trejo, Eva Carro, and Deborah J. Burke -- Insulin-like growth factor binding proteins in development / Josef V. Silha and Liam J. Murphy -- Growth hormone, insulin-like growth factors, and the female reproductive system / Carolyn A. Bondy and Jian Zhou -- Skeletal muscle / Ana M. Fernandez and Derek LeRoith -- Cardiovascular and respiratory systems / Åsa Tivesten and Jörgen Isgaard -- Hematopoiesis / Wilson Savino, Salete Smaniotto, and Mireille Dardenne -- Brain development / Gregory J. Popken ... [et al.] -- Regulation of vertebrate sensory organ development : a scenario for growth hormone and insulin-like growth factors action / Amelia Diaz-Casares ... [et al.] -- Role of insulin-like growth factors in neuronal plasticity and neuroprotection / Ignacio Torres Alemán -- Aging and life span / Holly M. Brown-Borg -- Interactions of insulin-like growth factor-I and estrogen in the brain / Pablo Mendez, Gloria Patricia Cardona-Gomez, and Luis Miguel García-Segura -- Cancer / Adda Grimberg -- Genetic basis of proportional short stature / Ángel Campos-Barros, Karen E. Heath, Jesús Argente -- Future perspectives : from stem cells and IGF biology to the clinic / Yvan Arsenijevic.
  • 2005 ScienceDirect
    Balch, William Edward; Der, Channing J.; Hall, A.
    Also available: Print – 2005
  • 2005 ScienceDirect
    Balch, William Edward; Der, Channing J.; Hall, A.
    Also available: Print – 2005
  • 2013 Springer Protocols
    Krieg, Thomas; Lukowski, Robert.
    NO/cGMP : the past, the present, and the future / Michael Russwurm [and 3 others] -- cGMP-dependent protein kinases (cGK) / Franz Hofmann and Jörg W. Wegener -- Enzyme assays for cGMP hydrolyzing phosphodiesterases / Sergei D. Rybalkin, Thomas R. Hinds, and Joseph A. Beavo -- Radioimmunoassay for the quantification of cGMP levels in cells and tissues / Ronald Jäger [and 5 others] -- Hyperspectral imaging of FRET-based cGMP probes / Thomas C. Rich [and 3 others] -- Visualization of cGMP with cGi biosensors / Martin Thunemann [and 4 others] -- Advances and techniques to measure cGMP in intact cardiomyocytes / Konrad R. Götz and Viacheslav O. Nikolaev -- Real-time monitoring the spatiotemporal dynamics of intracellular cGMP in vascular smooth muscle cells / Kara F. Held and Wolfgang R. Dostmann -- Methods for identification of cGKI substrates / Katharina Salb and Jens Schlossmann -- Approaches for monitoring PKG1[alpha] oxidative activation / Joseph Robert Burgoyne and Philip Eaton -- Analysis of cGMP signaling in adipocytes / Katja Jennissen [and 4 others] -- A genetic strategy for the analysis of individual axon morphologies in cGMP signalling mutant mice / Hannes Schmidt, Gohar Ter-Avetisyan, and Fritz G. Rathjen -- Receptor binding assay for NO-independent activators of soluble guanylate cyclase / Peter M. Schmidt and Johannes-Peter Stasch -- Direct intrathecal drug delivery in mice for detecting in vivo effects of cGMP on pain processing / Ruirui Lu and Achim Schmidtko -- The Geisler method : tracing activity-dependent cGMP plasticity changes upon double detection of mRNA and protein on brain slices / Wibke Singer, Hyun-Soon Geisler, and Marlies Knipper -- Detection of cGMP in the degenerating retina / Stylianos Michalakis [and 3 others].
  • 2009 ScienceDirect
    Burgess, Richard; Deutscher, Murray P.
    Also available: Print – 2009
  • 2010 CRCnetBASE
    Lundblad, Roger L.; Macdonald, Fiona M.
    "Offering a molecular perspective not available 20 years ago, [this edition] provides physical and chemical data on proteins, nucleic acids, lipids, and carbohydrates. Presented in an organized, concise, and simple-to-use format, this popular reference allows quick access to the most frequently used data. Covering a wide range of topics, from classical biochemistry to proteomics and genomics, it also details the properties of commonly used biochemicals, laboratory solvents, and reagents." --excerpts from book jacket.
  • 2010 Springer
    Jain, K. K.
    Technologies for discovery of biomarkers -- Biomarkers and molecular diagnostics -- Biomarkers for drug discovery and development -- Role of biomarkers in health care -- Biomarkers of cancer -- Biomarkers of disorders of the nervous system -- Biomarkers of cardiovascular disorders -- Biomarkers and personalized medicine -- Regulatory issues.
  • 2007 CRCnetBASE
    Langel, Ulo.
  • 2009 ScienceDirect
    Cummings, Richard D.; Pierce, J. Michael.
  • 2005 ScienceDirect
    Ahuja, Satinder; Garfin, David Edward.
  • 2011 Springer Protocols
    Fan, Teresa Whei-Mei; Higashi, Richard M.; Lane, Andrew N.
    Introduction to metabolomics / Teresa Whei-Mei Fan, Andrew N. Lane, and Richard M. Higashi -- Considerations of sample preparation for metabolomics investigation / Teresa Whei-Mei Fan -- Clinical aspects of metabolomics / Michael Bousamra II [and others] -- Structural mass spectrometry for metabolomics / Richard M. Higashi -- Metabolomic applications of inductively coupled plasma-mass spectrometry (ICP-MS) / Rob Henry and Teresa Cassel -- Principles of NMR for applications in metabolomics / Andrew N. Lane -- Novel NMR and MS approaches to metabolomics / Ian A. Lewis [and others] -- Metabolic flux analysis / Tae Hoon Yang -- Introduction to metabolic control analysis (MCA) / Maliackal Poulo Joy [and others] -- Application of tracer-based metabolomics and flux analysis in targeted cancer drug design / Marta Cascante, Vitaly Selivanov, and Antonio Ramos-Montoya -- Noninvasive fluxomics in mammals by nuclear magnetic resonance spectroscopy / Justyna Wolak [and others] -- Compositional analysis of phospholipids by mass spectrometry and phosphorus-31 nuclear magnetic resonance spectroscopy / M. Cecilia Yappert -- HumanCyc pathway-genome database and pathway tools software as tools for imaging and analyzing metabolomics data / Pedro Romero -- Metabolomics-edited transcriptomics analysis (META) / Teresa Whei-Mei Fan.
  • 2006- Wiley
    Messerschmidt, Albrecht.
    Iron -- NIckel -- Manganese -- Cobalt -- Molybdenum/Tungsten -- Copper -- Vanadium -- Zinc -- Calcium -- Magnesium.
  • 2009 CRCnetBASE
    Liu, Dongyou.
    Nucleic acids : structures, functions, and applications / Valentin V. Vlassov, Dimitrii V. Pyshnyi, and Pavel E. Vorobjev -- Isolation of viral DNA from cultures / Larry A. Hanson -- Isolation of viral RNA from cultures / Ling Zhao and Zhen F. Fu -- Preparation of viral samples for direct molecular applications / Grant S. Hansman -- Isolation of bacterial DNA from cultures / Dongyou Liu -- Isolation of bacterial RNA from cultures / Som Subhra Chatterjee and Trinad Chakraborty -- Isolation of plasmids / Armelle Bigot and Alain Charbit -- Preparation of bacterial samples for direct molecular applications / Knut Rudi ... [et al.] -- Isolation of nucleic acids from yeasts / Lakshman P. Samaranayake, Nipuna B. Parahitiyawa, and C. Jayampath Seneviratne -- Isolation of nucleic acids from filamentous fungi / Margaret E. Katz and Brian F. Cheetham -- Preparation of fungal specimens for direct molecular applications / Françoise Irlinger ... [et al.] -- Isolation of nucleic acids from protozoa / Vitaliano A. Cama and Lihua Xiao -- Isolation of nucleic acids from helminthes / Munehiro Okamoto and Akira Ito -- Isolation of nucleic acids from insects / Kun Yan Zhu -- Preparation of parasitic specimens for direct molecular applications / Huw Vaughan Smith and Rosely Angela Bergamin Nichols -- Isolation of nucleic acids from body fluids / Karen Page and Jacqueline Amanda Shaw -- Isolation of nucleic acids from soft tissues / Sergey Kovalenko -- Isolation of nucleic acids from hard tissues / Judith Weidenhofer and Jennifer A. Byrne -- Isolation of DNA from ancient samples / Paula F. Campos, Eske Willerslev, and M. Thomas P. Gilbert -- Preparation of forensic samples for direct molecular applications / Erin K. Hanson and Jack Ballantyne -- Isolation of nucleic acids from plants / Song Weining and Dongyou Liu -- Isolation of megabase-sized DNA fragments from plants / Meiping Zhang, Yaning Li, and Hong-Bin Zhang -- Purification of nucleic acids from gels / Chunfang Zhang and Theo Papakonstantinou.
  • 2005 Wiley
    Hartmann, Roland K.
    Part I. RNA Synthesis - Enzymatic RNA Synthesis, Ligation and Modification -- Chapter 1. Enzymatic RNA Synthesis using Bacteriophage T7 RNA Polymerase / Heike Gruegelsiepe, Astrid Schön, Leif A. Kirsebom and Roland K. Hartmann, p. 3-21 -- Chapter 2. Production of RNAs with Homogeneous 5' and 3' Ends / Mario Mörl, Esther Lizano, Dagmar K. Willkomm and Roland K. Hartmann, p. 22-35 -- Chapter 3. RNA Ligation using T4 DNA Ligase / Mikko J. Frilander and Janne J. Turunen, p. 36-52 -- Chapter 4. T4 RNA Ligase / Tina Persson, Dagmar K. Willkomm and Roland K. Hartmann, p. 53-74 -- Chapter 5. Co- and Post-Transcriptional Incorporation of Specific Modifications Including Photoreactive Groups into RNA Molecules / Nathan H. Zahler and Michael E. Harris, p. 75-85 -- Chapter 6. 3'-Terminal Attachment of Fluorescent Dyes and Biotin / Dagmar K. Willkomm and Roland K. Hartmann, p. 86-94 -- Part I. RNA Synthesis - Chemical RNA Synthesis -- Chapter 7. Chemical RNA Synthesis, Purification and Analysis / Brian S. Sproat, p. 95-111 -- Chapter 8. Modified RNAs as Tools in RNA Biochemistry / Thomas E. Edwards and Snorri T. H. Sigurdsson, p. 112-129 -- Part II. Structure Determination - Molecular Biology Methods -- Chapter 9. Direct Determination of RNA Sequence and Modification by Radiolabeling Methods / Olaf Gimple and Astrid Schön, p. 132-150 -- Chapter 10. Probing RNA Structures with Enzymes and Chemicals In Vitro and In Vivo / Eric Huntzinger, Maria Possedko, Flore Winter, Hervé Moine, Chantal Ehresmann and Pascale Romby, p. 151-171 -- Chapter 11. Study of RNA-Protein Interactions and RNA Structure in Ribonucleoprotein Particles / Virginie Marchand, Annie Mougin, Agnès Méreau and Christiane Branlant, p. 172-204 -- Chapter 12. Terbium(III) Footprinting as a Probe of RNA Structure and Metal-binding Sites / Dinari A. Harris and Nils G. Walter, p. 205-213 -- Chapter 13. Pb²⁺-induced Cleavage of RNA / Leif A. Kirsebom and Jerzy Ciesiolka, p. 214-228 -- Chapter 14. In Vivo Determination of RNA Structure by Dimethylsulfate / Christina Waldsich and Renée Schroeder, p. 229-237 -- Chapter 15. Probing Structure and Binding Sites on RNA by Fenton Cleavage / Gesine Bauer and Christian Berens, p. 238-249 -- Chapter 16. Measuring the Stoichiometry of Magnesium Ions Bound to RNA / A. J. Andrews and Carol Fierke, p. 250-258 -- Chapter 17. Nucleotide Analog Interference Mapping and Suppression: Specific Applications in Studies of RNA Tertiary Structure, Dynamic Helicase Mechanism and RNA-Protein Interactions, p. 259-293 / Olga Fedorova, Marc Boudvillain, Jane Kawaoka and Anna Marie Pyle -- Chapter 18. Nucleotide Analog Interference Mapping: Application to the RNase P System / Simona Cuzic and Roland K. Hartmann, p. 294-318 -- Chapter 19. Identification and Characterization of Metal Ion Binding by Thiophilic Metal Ion Rescue / Eric L. Christian, p. 319-344 -- Chapter 20. Identification of Divalent Metal Ion Binding Sites in RNA/DNA-metabolizing Enzymes by Fe(II)-mediated Hydroxyl Radical Cleavage / Yan-Guo Ren, Niklas Henriksson and Anders Virtanen, p. 345-353 -- Chapter 21. Protein-RNA Crosslinking in Native Ribonucleoprotein Particles / Henning Urlaub, Klaus Hartmuth and Reinhard Lührmann, p. 354-373 -- Chapter 22. Probing RNA Structure by Photoaffinity Crosslinking with 4-Thiouridine and 6-Thioguanosine / Michael E. Harris and Eric L. Christian, p. 374-384 -- Part II. Structure Determination - Biophysical Methods -- Chapter 23. Structural Analysis of RNA and RNA-Protein Complexes by Small-angle X-ray Scattering / Tao Pan and Tobin R. Sosnick, p. 385-397 -- Chapter 24. Temperature-Gradient Gel Electrophoresis of RNA / Detlev Riesner and Gerhard Steger, p. 398-414 -- Chapter 25. UV Melting, Native Gels and RNA Conformation / Andreas Werner, p. 415-427 -- Chapter 26. Sedimentation Analysis of Ribonucleoprotein Complexes / Jan Medenbach, Andrey Damianov, Silke Schreiner and Albrecht Bindereif, p. 428-437 -- Chapter 27. Preparation and Handling of RNA Crystals / Boris François, Aurélie Lescoute-Phillips, Andreas Werner and Benoît Masquida, p. 438-452 -- Part II. Structure Determination - Fluorescence and Single Molecule Studies -- Chapter 28. Fluorescence Labeling of RNA for Single Molecule Studies / Filipp Oesterhelt, Enno Schweinberger and Claus Seidel, p. 453-474 -- Chapter 29. Scanning Force Microscopy and Scanning Force Spectroscopy of RNA / Wolfgang Nellen, p. 475-487 -- Part III. RNA Genomics and Bioinformatics - Fluorescence and Single Molecule Studies -- Chapter 30. Comparative Analysis of RNA Secondary Structure: 6S RNA / James W. Brow and J. Christopher Ellis, p. 490-512 -- Chapter 31. Secondary Structure Prediction / Gerhard Steger, p. 513-535 -- Chapter 32. Modeling the Architecture of Structured RNAs within a Modular and Hierarchical Framework, p. 536-545 / Benoît Masquida and Eric Westhof -- Chapter 33. Modeling Large RNA Assemblies using a Reduced Representation, p. 546-559 / Jason A. Mears, Scott M. Stagg and Stephen C. Harvey -- Chapter 34. Molecular Dynamics Simulations of RNA Systems / Pascal Auffinger and Andrea C. Vaiana, p. 560-576 -- Chapter 35. Seeking RNA Motifs in Genomic Sequences / Matthieu Legendre and Daniel Gautheret, p. 577-594 -- Chapter 36. Approaches to Identify Novel Non-messenger RNAs in Bacteria and to Investigate their Biological Functions: RNA Mining / Jörg Vogel, E. Gerhart and H. Wagner, p. 595-613 -- Chapter 37. Approaches to Identify Novel Non-messenger RNAs in Bacteria and to Investigate their Biological Functions: Functional Analysis of Identified Non-mRNAs / E. Gerhart, H. Wagner and Jörg Vogel, p. 614-642 -- Chapter 38. Experimental RNomics: A Global Approach to Identify Non-coding RNAs in Model Organisms / Alexander Hüttenhofer, p. 643-654 -- Chapter 39. Large-scale Analysis of mRNA Splice Variants by Microarray / Young-Soo Kwon, Hai-Ri Li and Xiang-Dong Fu, p. 655-663 -- Part IV. Analysis of RNA Function 665 - RNA-Protein Interactions in vitro -- Chapter 40. RNA-Protein Interactions in vitro / Steffen Schiffer, Sylvia Rösch, Bettina Späth, Markus Englert, Hildburg Beier and Anita Marchfelder, p. 666-675 -- Chapter 41. Biotin-based Affinity Purification of RNA-Protein Complexes / Zsofia Palfi, Jingyi Hui and Albrecht Bindereif, p. 676-693 -- Chapter 42. Immunoaffinity Purification of Spliceosomal and Small Nuclear Ribonucleoprotein Complexes / Cindy L. Will, Evgeny M. Makarov, Olga V. Makarova and Reinhard Lührmann, p. 694-709 -- Chapter 43. Northwestern Techniques for the Identification of RNA-binding Proteins from cDNA Expression Libraries and the Analysis of RNA-Protein Interactions / Ángel Emilio Martínez De Alba, Michela Alessandra Denti and Martin Tabler, p. 710-728 -- Part IV. Analysis of RNA Function 665 - RNA-Protein Interactions in vivo -- Chapter 44. Fluorescent Detection of Nascent Transcripts and RNA-binding Proteins in Cell Nuclei, p. 729-736 / Jennifer A. Geiger and Karla M. Neugebauer -- Chapter 45. Identification and Characterization of RNA-binding Proteins through Three-hybrid Analysis / Felicia Scott and David R. Engelke, p. 737-754 -- Chapter 46. Analysis of Alternative Splicing In Vivo using Minigenes / Yesheng Tang, Tatyana Novoyatleva, Natalya Benderska, Shivendra Kishore, Alphonse Thanaraj and Stefan Stamm, p. 755-782 -- Part IV. Analysis of RNA Function 665 - SELEX -- Chapter 47. Artificial Selection: Finding Function amongst Randomized Sequences / Ico de Zwart, Catherine Lozupone, Rob Knight, Amanda Birmingham, Mali Illangasekare, Vasant Jadhav, Michal Legiewicz, Irene Majerfeld, Jeremy Widmann and Michael Yarus, p. 783-806 -- Chapter 48. Aptamer Selection against Biological Macromolecules: Proteins and Carbohydrates / C. Stefan Vörtler and Maria Milovnikova, p. 807-839 -- Chapter 49. In Vivo SELEX Strategies / Thomas A. Cooper, p. 840-852 -- Chapter 50. In Vitro Selection against Small Targets / Dirk Eulberg, Christian Maasch, Werner G. Purschke and Sven Klussmann, p. 853-877 -- Chapter 51. SELEX Strategies to Identify Antisense and Protein Target Sites in RNA or Heterogeneous Nuclear Ribonucleoprotein Complexes / Martin Lützelberger, Martin R. Jakobsen and Jørgen Kjems, p. 878-894 -- Chapter 52. Gene Silencing Methods for Mammalian Cells: Application of Synthetic Short Interfering RNAs / Matthias John, Anke Geick, Philipp Hadwiger, Hans-Peter Vomlocher and Olaf Heidenreich, p. 896-909 -- Appendix. UV Spectroscopy for the Quantitation of RNA, p. 910-913.
  • 2011 Springer
    Hughes, T. R.
    Introduction to "a handbook of transcription factors" / T.R. Hughes -- An overview of prokaryotic transcription factors : a summary of function and occurrence in bacterial genomes / A.S. Seshasayee, K. Sivaraman and N.M. Luscombe -- A catalogue of eukaryotic transcription factor types, their evolutionary origin, and species distribution / M.T. Weirauch and T.R. Hughes -- Function and evolution of C2H2 zinc finger arrays / L. Stubbs, Y. Sun and D. Caetano-Anolles -- Homeodomain subtypes and functional diversity / T.R. Burglin -- Nuclear receptors : Small molecule sensors that coordinate growth, metabolism and reproduction / K. Pardee, A.S. Necakov and H. Krause -- Methods for analysis of transcription factor DNA-binding specificity in vitro / A. Jolma and J. Taipale -- Identification of transcription factor-DNA interactions in vivo / D.T. Odom -- How transcription factors identify regulatory sites in genomic sequence / Y. Field, E. Sharon and E. Segal -- Transcription factor binding sites and other features in human and Drosophila proximal promoters / C. Vinson, R. Chatterjee and P. Fitzgerald -- Interactions of transcription factors with chromatin / H. van Bakel -- Transcription factor effector domains / S. Frietze and P.J. Farnham -- Large-scale nuclear architecture and transcriptional control / J.M. Vaquerizas, A. Akhtar and N.M. Luscombe.
  • 2015 (30th ed.). AccessMedicine
    29th ed., 2012 AccessMedicine
    Bender, David A.; Botham, Kathleen M.; Granner, Daryl K.; Harper, Harold A.; Kennelly, Peter J.; Mayes, Peter A.; Murray, Robert K.; Rodwell, Victor W.; Weil, P. Anthony.
    Also available: Print – 2003-<2015>
  • 2014 ScienceDirect
    Komoda, Tsugikazu.
    The HDL Handbook: Biological Functions to Clinical Implications brings laboratory research in HDL from bench to bedside in this needed resource for researchers and clinicians studying cholesterol, lipids, epidemiology, biochemistry, molecular medicine, and pathophysiology of cardiovascular diseases. In addition, researchers and clinicians working with an aging population, corporate researchers, post-doctorates; medical students and graduate students will find this publication useful because the scope of coverage includes basic science, genetics, epidemiology, and treatment of HDL cholesterol as well as potential targets to modify HDL cholesterol. Provides bench-to-bedside coverage of HDL with thorough coverage of basic science, genetics, epidemiology, and treatment Presents a complete update with six new chapters on the latest advances in HDL cholesterol research with international perspective New chapters on proteomics, clinical impact of LCAT in HDL metabolism, and an in-depth discussion of potential targets to modify HDL provide a translational reference for clinicians.
  • 2013 Springer Protocols
    Vivanco, Fernando.
    Large-scale characterization of the murine cardiac proteome / Jake Cosme, Andrew Emili, and Anthony O. Gramolini -- Determining protein concentrations of the human ventricular proteome / Arjen Scholten and Albert J.R. Heck -- Multidimensional protein identification technology for direct-tissue proteomics of heart / Dario Di Silvestre, Francesca Brambilla, and Pier Luigi Mauri -- Global protein quantification of mouse heart tissue based on the SILAC Mouse / Anne Konzer [and others] -- Global proteomic profiling and enrichment maps of dilated cardiomyopathy / Ruth Isserlin, Daniele Merico, and Andrew Emili -- Characterization of the human myocardial proteome in dilated cardiomyopathy by label-free quantitative shotgun proteomics of heart biopsies / Elke Hammer, Katrin Darm, and Uwe Völker -- Systems proteomics of healthy and diseased chromatin / Haodong Chen [and others] -- Proteomic analysis of interstitial aortic valve cells acquiring a pro-calcific profile / Millioni Renato [and others] -- Differential protein expression analysis of degenerative aortic stenosis by iTRAQ labeling / Sergio Alonso-Orgaz [and others] -- Characterization of zebrafish cardiac proteome using online pH Gradient SCX-RP HPLC-MS/MS Platform / Jiang Zhang [and others] -- Proteomic analysis of brain mitochondrial proteome and mitochondrial complexes / Ana Lopez-Campistrous and Carlos Fernandez-Patron -- Oxidative modifications of mitochondria complex II / Liwen Zhang [and others] -- Detection of O-GlcNAc modifications on cardiac myofilament proteins / Genaro A. Ramirez-Correa [and others] -- Quantification of mitochondrial S-nitrosylation by CysTMT 6 Switch Assay / Christopher I. Murray [and others] -- Identification of thioredoxin target protein networks in cardiac tissues of a transgenic Mouse / Cexiong Fu [and others] -- Using pure protein to build a multiple reaction monitoring mass spectrometry assay for targeted detection and quantitation / Eric Grote [and others] -- Sequential extraction methodology for cardiac extracellular matrix prior to proteomics analysis / Javier Barallobre-Barreiro [and others] -- Optimized method for identification of the proteomes secreted by cardiac cells / Miroslava Stastna and Jennifer E. Van Eyk -- Secretome of human aortic valves / Fernando de la Cuesta [and others] -- Comparative study of immunodepletion and equalization methods for aortic stenosis human plasma / Felix Gil-Dones [and others].
  • 2007 Springer
    Asea, Alexzander A. A.; De Maio, Antonio.
  • 2008 Springer
    Asea, Alexzander A. A.; Brown, Ian R.
  • 2010 Springer
    Asea, Alexzander A. A.; Pedersen, Bente Klarlund.
  • 2007 Springer
    Ciocca, Daniel R.; Calderwood, Stuart K.; Sherman, Michael Y.
  • 2014 Springer
    Blatch, Gregory L.; Shonhai, Addmore.
    The Importance of Molecular Chaperones in Survival and Pathogenesis of the Malaria Parasite Plasmodium falciparum / Jude Przyborski -- General Structural and Functional Features of Molecular Chaperones / Adrienne L. Edkins and Aileen Boshoff -- The Role of Hsp70s in the Development and Pathogenicity of Plasmodium Species / Addmore Shonhai -- Role of the Hsp40 Family of Proteins in the Survival and Pathogenesis of the Malaria Parasite / Eva-Rachele Pesce, Alexander G. Maier and Gregory L. Blatch -- Role of Hsp90 in Plasmodium falciparum Malaria / Dea Shahinas and Dylan R. Pillai -- The Role of Parasite Heat Shock Proteins in Protein Trafficking and Host Cell Remodeling / Paul R. Gilson, Sarah C. Charnaud and Brendan S. Crabb -- Role of Heat Shock Proteins in Immune Modulation in Malaria / Evelyn Böttger and Gabriele Multhoff -- Establishment of Plasmodium falciparum Extracellular Compartments in its Host Erythrocyte / Alassane Mbengue, Laurence Berry and Catherine Braun-Breton -- Chaperones and Proteases of Plasmodium falciparum / Kaiyin Liu and Walid A. Houry -- Heat Shock Proteins as Targets for Novel Anti-Malarial Drugs / Manish Grover, Shweta Chaubey and Utpal Tatu -- Heat Shock Proteins of Malaria: What Do We Not Know, and What Should the Future Focus Be? / Addmore Shonhai and Gregory L. Blatch.
  • 2011 Springer
    Xie, Jingwu.
  • 2015 Springer Protocols
    Riobo, Natalia A.
    Luciferase reporter assays to study transcriptional activity of hedgehog signaling in normal and cancer cells / Silvia Pandolfi and Barbara Stecca -- Measuring expression levels of endogenous gli genes by immunoblotting and real-time PCR / Pawel Niewiadomski and Rajat Rohatgi -- Quantitative immunoblotting of endogenous hedgehog pathway components / Shohreh F. Farzan and David J. Robbins -- Measuring Gli2 phosphorylation by selected reaction monitoring mass spectrometry / Robert Ahrends, Pawel Niewiadomski, Mary N. Teruel, and Rajat Rohatgi -- Rapid screening of Gli2/3 mutants using the Flp-In system / Pawel Niewiadomski and Rajat Rohatgi -- Insights into gli factors ubiquitylation methods / Paola Infante, Romina Alfonsi, and Lucia Di Marcotullio -- Determination of acetylation of the gli transcription factors / Sonia Coni, Laura Di Magno, and Gianluca Canettieri -- Efficient detection of indian hedgehog during endochondral ossification by whole-mount immunofluorescence / João Francisco Botelho, Daniel Smith-Paredes, and Veronica Palma A. -- Methods for detection of Ptc1-driven LacZ expression in adult mouse skin / Donna M. Brennan-Crispi, My G. Mahoney, and Natalia A. Riobo -- Determination and analysis of cellular metabolic changes by noncanonical hedgehog signaling / Raffaele Teperino and John Andrew Pospisilik.
  • 2007 Springer Protocols
    Horabin, Jamila I.
    Purifying the hedgehog protein and its variants / Darren P. Baker, Frederick R. Taylor, and R. Blake Pepinsky -- Application of sonic hedgehog to the developing chick limb / Eva Tieck and Cheryll Tickle -- Manipulation of hedgehog signaling in Xenopus by means of embryo microinjection and application of chemical inhibitors / Thomas Hollemann .. [et al.] -- Isolation of rat telencephalic neural explants to assay Shh GABAergic interneuron differentiation-inducing activity / Rina Mady and Jhumku D. Kohtz -- Genetic analysis of the vertebrate hedgehog-signaling pathway using muscle cell fate specification in the zebrafish embryo / Sudipto Roy -- Efficient manipulation of hedgehog/GLI signaling using retroviral expression systems / Maria Kasper ... [et al.] -- Cell surface marker and cell cycle analysis, hedgehog signaling, and flow cytometry / Krsting Detmer and Ronald E. Garner -- Detecting tagged hedgehog with intracellular and extracellular immunocytochemistry for functional analysis / Ainhoa Callejo, Luis Quijada, and Isabel Guerrero -- Confocal analysis of hedgehog morphogenetic gradient coupled with fluorescent in situ hybridization of hedgehog target genes / Armel Gallet and Pascal P. Thérond -- RNA in the hedgehog signaling pathway : pFRiPE, a vector for temporally and spatially controlled RNAi in Drosophila / Eric Marois and Suzanne Eaton -- Germline clone analysis for maternally acting Drosophila hedgehog components / Erica M. Selva and Beth E. Stronach -- Clone analysis of hedgehog signaling in Drosophila somatic tissues / Christing M. Bankers and Joan E. Hooper -- GAL4/UAS targeted gene expression for studying Drosophila hedgehog signaling / Denise Bussona nd Anne-Marie Pret -- Biochemical fractionation of Drosophila cells / Melanie Stegman and David Robbins -- Using immunoprecipitation to study protein-protein interactions in the hedgehog-signaling pathway / Chao Ton and Jin Jiang -- Sequence analyses to study the evolutionary history and cis-regulatory elements of hedgehog genes / Ferenc Müller and Anne-Gaelle Borycki.
  • 2006 Springer
    Ruiz i Altaba, Ariel.
    How the hedgehog outfoxed the crab: interference with HEDGEHOG-GLI signaling as anti-cancer therapy? / Ariel Ruiz i Altaba -- The patched receptor: switching on/off the hedgehog signaling pathway / Luis Quijada ... [et al.] -- Making a morphogenetic gradient / Henk Roelink -- Spatial and temporal regulation of hair follicle progenitors by hedgehog signaling / Anthony E. Oro -- Mode of PTCH1/Ptch1-associated tumor formation: insights from mutant Ptch1 mice / Heidi Hahn -- Basal cell carcinomas, hedgehog signaling, and the Ptch1+/- mouse / Ervin Epstein, Jr. -- GLI genes and their targets in epidermal development and disease / Fritz Aberger and Anna-Maria Frischauf -- Splitting hairs: dissecting the roles of Gli activator and repressor functions during epidermal development and disease / Pleasantine Mill and Chi-Chung Hui -- Shh expression in pulmonary injury and disease / Paul M. Fitch ... [et al.] -- Human correlates of GL13 function / Leslie G. Biersecker -- From oligodactyly to polydactyly: role of Shh and Gli3 in limb morphogenesis / Chin Chiang -- The genetics of Indian hedgehog / M. Elizabeth McCready and Dennis E. Bulman -- Sonic hedgehog signaling in craniofacial development / Dwight Cordero, Minal Tapadia and Jill A. Helms -- Important role of Shh controlling Gli3 functions during the dorsal-ventral patterning of the telencephalon / Jun Motoyama and Kazushi Aoto -- Regulation of early events in cell cycle progression by hedgehog signaling in CNS development and tumorigenesis / Anna Marie Kenney and David H. Rowitch -- Modulating the hedgehog pathway in diseases / Frederic J. de Sauvage and Lee L. Rubin -- Hedgehog signaling in endodermally derived tumors / Marina Pasca di Magliano and Matthias Hebrok.
  • 2012 Springer
    Lever, Rebecca; Mulloy, Barbara; Page, C. P.
    History of heparin / T.W. Barrowcliffe -- Heparin biosynthesis / Pernilla Carlsson and Lena Kjellén -- Anticoagulant and antithrombotic mechanisms of heparin / Elaine Gray, John Hogwood, and Barbara Mulloy -- Standardisation of unfractionated and low-molecular-weight heparin / Elaine Gray -- Structure and physicochemical characterisation of heparin / Barbara Mulloy -- Case study: contamination of heparin with oversulfated chondroitin sulfate / Edward K. Chess ... [et al.] -- Low-molecular-weight heparins: differential characterization/physical characterization / Marco Guerrini and Antonella Bisio -- Heparin and heparan sulfate: analyzing structure and microheterogeneity / Zachary Shriver ... [et al.] -- Heparin in the prophylaxis and treatment of venous thromboembolism and other thrombotic diseases / Paolo Gresele, Chiara Busti, and Gloria Paganelli -- Adverse effects of heparin / S. Alban -- Neutralization of heparin activity / Menaka Pai and Mark A. Crowther -- Non-anticoagulant effects of heparin: an overview / Rebecca Lever and Clive P. Page -- Glycosaminoglycan and chemokine/growth factor interactions / Janis Shute -- Glycosaminoglycans and neuroprotection / B. Dudas and K. Semeniken -- Heparan sulphate: a heparin in miniature / J.T. Gallagher -- Heparin mimetics / Deirdre R. Coombe and Warren C. Kett -- Hyaluronic acid / Luigi Allegra, Sabrina Della Patrona, and Giuseppe Petrigni -- Semi-synthetic heparinoids / P. Oreste and G. Zoppetti -- Heparin-like entities from marine organisms / S. Colliec-Jouault, C. Bavington, and C. Belbarre-Ladrat.
  • 2015 Springer
    Akasaka, Kazuyuki; Matsuki, Hitoshi.
    Part I. Why and how proteins denature under prssure? -- Part II. Volume, compressibility, fluctuation and interaction in proteins -- Part III. Pressure and functional sub-states in proteins -- Part IV. Pressure and protein folding and assembly -- Part V. Pressure effects on biological membranes -- Part VI. Pressure adaptation and tolerance of proteins and living organisms -- Part VII. High pressure food processing and sterilization -- Part VIII. Pressure effects on motility, physiology and health -- Part IX. Methodology.
  • 2009 Springer Protocols
    Doyle, Sharon A.
    Hexahistidine-tagged maltose-binding protein as a fusion partner for the production of soluble recombinant proteins in Escherichia coli / Brian P. Austin, Sreedevi Nallamsetty, and David S. Waugh -- PHB-intein-mediated protein purification strategy / Alison R. Gillies, Mahmoud Reza Banki, and David W. Wood -- High-throughput biotinylation of proteins / Brian K. Kay, Sang Thai, and Veronica V. Volgina -- High-throughput insect cell protein expression applications / Mirjam Buchs ... [et al.] -- High-throughput protein expression using cell-free system / Kalavathy Sitaraman and Deb K. Chatterjee -- The production of glycoproteins by transient expression in mammalian cells / Joanne E. Nettleship, Nahid Rahman-Huq, and Raymond J. Owens -- High-throughput expression and detergent screening of integral membrane proteins / Said Eshaghi -- Cell-free expression for nanolipoprotein particles : building a high-throughput membrane protein solubility platform / Jenny A. Cappuccio ... [et al.] -- Expression and purification of soluble His₆-tagged TEV protease / Joseph E. Tropea, Scott Cherry, and David S. Waugh -- High-throughput protein concentration and buffer exchange : comparison of ultrafiltration and ammonium sulfate precipitation / Priscilla A. Moore and Vladimir Kery. High-throughput protein production (HTTP) : a review of enabling technologies to expedite protein production / Jim Koehn and Ian Hunt -- Designing experiments for high-throughput protein expression / Stephen P. Chambers and Susanne E. Swalley -- Gateway cloning for protein expression / Dominic Esposito, Leslie A. Garvey, and Chacko S. Chakiath -- Flexi vector cloning / Paul G. Blommel ... [et al.] -- The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning / Nick S. Berrow, David Alderton, and Raymond J. Owens -- The polymerase incomplete primer extension (PIPE) method applied to high-throughput cloning and site-directed mutagenesis / Heath E. Klock and Scott A. Lesley -- A family of LIC vectors for high-throughput cloning and purification of proteins / William H. Eschenfeldt ... [et al.] -- "System 48" high-throughput cloning and protein expression analysis / James M. Abdullah, Andrzej Joachimiak, and Frank R. Collart -- Automated 96-well purification of hexahistidine-tagged recombinant proteins on MagneHis Ni²⁺-particles / Chiann-Tso Lin, Priscilla A. Moore, and Vladimir Kery -- E. coli and insect cell expression, automated purification and quantitative analysis / StephenP. Chambers ... [et al.].
  • 2010
    Wilcox, R. Bruce.
    Acid-base relationships -- Amino acids and proteins -- Enzymes -- Citric acid cycle and oxidative phosphorylation -- Carbohydrate metabolism -- Lipid metabolism -- Amino acid metabolism -- Nucleotide metabolism -- Nutrition -- Gene expression -- Biochemical technology -- Hormones.
  • 2003 ScienceDirect
    Dutta, Rinku; Inouye, Masayori.
  • 2013 CRCnetBASE
    Exbrayat, J. M.
    Section 1. Histological and histochemical methods with light microscopy -- section 2. Cytological methods in electron microscopy -- section 3. Image quantification.
  • 2006 Springer
    Verdin, Eric.
    Histone deacetylase 1 / Dominique Meunier and Christian Seiser -- Biochemistry of multiprotein HDAC complexes / Alejandro Vaquero, Michael Scher, and Danny Reinberg -- The biology of HDAC3 / Edward Seto -- The biology of HDAC8, a unique class I histone deacetylase / David Waltregny and Vincent Castronovo -- Regulation of muscle gene expression by histone deacetylases / Timothy A. McKinsey and Eric N. Olson -- The class IIA histone deacetylases: functions and regulation / Herbert G. Kasler and Eric Verdin -- Histone deacetylases in the response to misfolded proteins / J. Andrew McKee and Tso-Pang Yao -- Evolution of sirtuins from archaea to vertebrates / Roy A. Frye -- Structure of the Sir2 family of NAD+-dependent histone/protein deacetylases / Kehao Zhao and Ronen Marmorstein -- The enzymology of Sir2 proteins / Margie T. Borra and John M. Denu -- The class III protein deacetylases: homologs of yeast Sir2P / Bjoern Schwer ... [et al.] -- HDAC inhibitors: discovery, development, and clinical impacts / Akihiro Ito, Norikazu Nishino, and Mitsuaki Yoshida -- Cell cycle targets of histone deacetylase inhibitors / Brian Gabrielli -- HDAC inhibitors: an emerging anticancer therapeutic strategy / Paul Kwon, Meier Hsu, Dalia Cohen, and Peter Atadja.
  • 2005 Springer
    Belfort, Marlene.
  • 2013 Springer
    Jakubowski, Hieronim.
    Excess of homocysteine, a product of the metabolism of the essential amino acid methionine, is associated with poor health, is linked to heart and brain diseases in general human populations, and accelerates mortality in heart disease patients. Neurological and cardiovascular abnormalities occur in patients with severe genetic hyperhomocysteinemia and lead to premature death due to vascular complications. Although it is considered a non-protein amino acid, studies over the past dozen years have discovered mechanisms by which homocysteine becomes a component of proteins. Homocysteine-containing proteins lose their normal biological function and become auto-immunogenic and pro-thrombotic. In this book, the author, a pioneer and a leading contributor to the field, describes up-to date studies of the biological chemistry of homocysteine-containing proteins, as well as pathological consequences and clinical implications of their formation. This is a comprehensive account of the broad range of basic science and medical implications of homocysteine-containing proteins for health and disease.
  • 2010 Springer
    Calabrese, Edward J.; Mattson, Mark Paul.
  • 2008 ClinicalKey
    Litwack, Gerald.
    This textbook is specifically designed for upper-division undergraduate or graduate students in life science or pre-medical majors including dentistry or pharmacology, who are required to take a biochemistry or medical biochemistry course, but who are not necessarily biochemistry majors. The book adopts a unique approach to the topic compared with other biochemistry textbooks currently available, in that each biochemical subject is introduced by a human disease relating the biochemical principles to be developed in that chapter. The goal is to make biochemistry more meaningful to the student who is not normally shown the connection between biochemistry and medicine. This book: includes an abundance of figures; emphasizes human biochemistry; and introduces each chapter with a relevant disease or clinical relationship.
  • 2005 CRCnetBASE
    Coughtrie, Michael W. H.; Pacifici, Gian Maria.
    Nomenclature and molecular biology of the human sulfotransferase family / Rebecca Blanchard -- Structure and function of sulfotranserases / Jyrki Taskinen and W.H. Coughtrie -- PAPS and sulfoconjucation / Nancy B. Schwartz -- Sulfate conjugation: pharmacogenetics and pharmacogenomics / by Weinshilboum & Adjei -- Molecular cloning of the human cytosolic sulfotransferases / by C.N.Falany ... [et al.] -- Sulfotransferases in the human fetus and neonate / by Stanley, Coughtri and Hume -- Sulfation of thyroid hormones / by Monique H.A. Kester, Michael W.H. Coughtrie and Theo J. Vissor -- Estrogen sulfotransferase in breast cancer / by Pasqualini and Chetrite -- Sulfation of drugs / by Pacifici -- Human sult1a sulfotransferases / by Nadine Hempel ... [et al.] -- Hydroxysteroid sulfotransferases sult2a1, sult2b1a and sult2b1b / by Charles A. Strott -- Species differences in cytosol sulfotoransgerases / Kiyoshi Nagata, Miki Shimada, and Yasushi Yamazoe -- Activiation and inactivation of carconogens and mutagens by human sulfotranserases / by Hansruedi Glatt.
  • 2015 ScienceDirect
    pt. B ScienceDirect
    Cadenas, Enrique; Packer, Lester.
  • 2008 Springer
    Tachezy, Jan.
    Anaerobic eukaryotes in pursuit of phylogenetic normality: the evolution of hydrogenosomes and mitosomes / W. Martin -- Protein import into hydrogenosomes and mitosomes / S. D. Dyall, P. Doležal -- Structure of the hydrogenosome / M. Benchimol -- Hydrogenosomes of anaerobic ciliates / J. H. P. Hackstein ... [et al.] -- Metabolism of trichomonad hydrogenosomes / I. Hrdý, J. Tachezy, M. Müller -- Hydrogenosomes of anaerobic chytrids: an alternative way to adapt to anaerobic environments / J. H. P. Hackstein ... [et al.] -- The proteome of T. vaginalis hydrogenosomes / K. Henze -- Hydrogenosome: the site of 5-nitroimidazole activation and resistance / J. Kulda, I. Hrdý -- Mitosomes in parasitic protists / J. Tachezy, O. Šmíd -- The mitochondrion-related organelle of Cryptosporidium parvum / J. S. Keithly -- Mitochondrial remnant in blastocystis / N. Yarlett, K. S. W. Tan -- Possible mitochondria-related organelles in poorly-studied "amitochondriate" eukaryotes / V. Hampl, A. G. B. Simpson.
  • 2011 Springer
    Sorrentino, Matthew J.
    Cardiovascular Risk Assessment -- The Metabolic Syndrome -- The Role of Imaging in Cardiovascular Risk Assessment -- Inherited Lipoprotein Disorders -- Advanced Lipid Testing -- Therapeutic Lifestyle Change for the Prevention and Treatment of Hyperlipidemia and Coronary Artery Disease -- Drug Therapy for Dyslipidemia -- Hypertriglyceridemia -- Isolated Low HDL -- Lipoprotein(a) -- HIV with Dyslipidemia -- Treatment of Dyslipidemia in Patients with Renal Insufficiency and End-Stage Renal Disease.
  • 2013
    Senechal, Marjorie.
    "In the vein of A Beautiful Mind, The Man Who Loved Only Numbers, and Rosalind Franklin: The Dark Lady of DNA, this volume tells the poignant story of the brilliant, colorful, controversial mathematician named Dorothy Wrinch. Drawing on her own personal and professional relationship with Wrinch and archives in the United States, Canada, and England, Marjorie Senechal explores the life and work of this provocative, scintillating mind. Senechal portrays a woman who was learned, restless, imperious, exacting, critical, witty, and kind. A young disciple of Bertrand Russell while at Cambridge, the first women to receive a doctor of science degree from Oxford University, Wrinch's contributions to mathematical physics, philosophy, probability theory, genetics, protein structure, and crystallography were anything but inconsequential. But Wrinch, a complicated and ultimately tragic figure, is remembered today for her much publicized feud with Linus Pauling over the molecular architecture of proteins. Pauling ultimately won that bitter battle. Yet, Senechal reminds us, some of the giants of mid-century science--including Niels Bohr, Irving Langmuir, D'Arcy Thompson, Harold Urey, and David Harker--took Wrinch's side in the feud. What accounts for her vast if now-forgotten influence? What did these renowned thinkers, in such different fields, hope her model might explain? Senechal presents a sympathetic portrait of the life and work of a luminous but tragically flawed character. At the same time, she illuminates the subtler prejudices Wrinch faced as a feisty woman, profound culture clashes between scientific disciplines, ever-changing notions of symmetry and pattern in science, and the puzzling roles of beauty and truth" -- Provided by publisher. "A biography of Dorothy Wrinch" -- Provided by publisher.
  • 2014 Springer
    Gullberg, Donald E.
    "The integrin family is composed of 24 members and approximately ten years ago (2003) we published a book devoted to the nine I domain integrin subunits. In this second edition, I am pleased that most of the original authors have been able to contribute to the updated version. I domain containing integrins include collagen receptors and leukocyte receptors. In 2003 the knockout mouse phenotypes for all of the I domain integrins had not yet been published; they are now, and are summarized and discussed in this edition. Interestingly, a recent 10 integrin mutation in dogs has indicated that collagen-binding integrins in the musculoskeletal system might have much more severe phenotypes in larger animals/humans compared to the mild integrin phenotypes observed in collagen-binding integrin deficient mice. This finding is further discussed in the book. In the cancer field, the microenvironment is taking center stage, and here collagen receptors on fibroblasts are predicted to play important roles in paracrine signaling, in regulating tissue stiffness and matrix remodeling. New technologies, new mouse models in combination with analyses of I integrins in larger animals/humans are thus predicted to increase our knowledge about this group of receptors. With this in mind we look forward to another 10 years of research with I domain integrins."--Publisher's wevsite.
    Also available: Print – 2014
  • 2011
    Firestone, Ari Joseph; Chen, James K.; Meyer, Tobias; Nelson, W. J.; Scott, Matthew P.
    Proper activation of Hh signaling is a critical mediator of metazoan development whereas aberrant activation, in post-embryonic tissues, is associated with initiation or progression of numerous common tumors. In chapter 1 of this dissertation I review the history, development, and status of extant small-molecule Hh inhibitors. I discuss limitations in the current approaches utilized in the discovery of these compounds, and the successes and challenges associated with tackling this pathway with targeted therapeutics. In chapter 2, I detail our progress using high-throughput screening to discover new small-molecule inhibitors of Hh signaling by specifically targeting elements of the pathway downstream of the transmembrane protein Smoothened (Smo), the target of the vast majority of existing inhibitors. These efforts resulted in the identification and preliminary characterization of four small molecules, each of which displays a distinct and novel combination of effects on various downstream signaling processes including formation of the primary cilia, the Hh-dependent regulation of Gli proteolytic-processing, and activation of Gli transcription factors. These compounds therefore represent valuable probes for further dissection of mechanisms of Hh signaling downstream of Smo and possible leads in the development of mechanistically novel Hh targeted chemotherapeutics. The third chapter continues with an in depth study of the Hh antagonist capable of preventing formation of primary cilia. We name this compound and its structurally and functionally related congeners ciliobrevins. We observe that acute treatment of cells with ciliobrevins leads to an accumulation of proteins in the distal tips of primary cilia, a phenotype that is consistent with inhibition of dynein-dependent retrograde protein trafficking. Ciliobrevin treatment also causes defects in mitotic spindle formation and the subcellular movement of organelles that are reminiscent of those observed under dynein loss of function conditions. Finally we show that ciliobrevins inhibit dynein-dependent microtubule gliding and dynein ATPase activities in vitro, demonstrating that this motor protein represents the critical physiological target of these small-molecule Hh inhibtors. This dissertation is concluded by a broader review of the use and development of small-molecules to control additional developmental signaling pathways and processes such as differentiation and self-renewal. Alterations in fate choice are central to a plethora of human diseases and chemically manipulating these will be a critical aspect in the advancement of medical technology from the treatment of tumors, to the advent of regenerative therapies.
  • 2012 ScienceDirect
    Conn, P. Michael.
    Section 1. Viruses, microbes, and parasites. Microbial cells analysis by atomic force microscopy / David Alsteens ; Understanding parasite transmission through imaging approaches / Mirko Singer and Freddy Frischknecht ; Multiphoton microscopy applied for real-time intravital imaging of bacterial infections in vivo / Ferdinand X. Choong ... [et al.] ; Analysis of virus entry and cellular membrane dynamics by single particle tracking / Helge Ewers and Mario Schelhaas ; Imaging of live malaria blood stage parasites / Christof Grüring and Tobias Spielmann ; Escherichia coli K1 invasion of human brain microvascular endothelial cells / Lip Nam Loh and Theresa H. Ward -- Section 2. Disease states. Real-time imaging after cerebral ischemia : model systems for visualization of inflammation and neuronal repair / Pierre Cordeau and Jasna Kriz ; In vivo real-time visualization of leukocytes and intracellular hydrogen peroxide levels during a zebrafish acute inflammation assay / Luke Pase, Cameron J. Nowell, and Graham J. Lieschke ; Imaging protein oligomerization in neurodegeneration using bimolecular fluorescence complementation / Federico Herrera, Susana Gonçalves, and Tiago Fleming Outeiro ; 3D/4D functional imaging of tumor-associated proteolysis : impact of microenvironment / Kamiar Moin ... [et al.] -- Section 3. Other techniques. Live cell imaging in live animals with fluorescent proteins / Robert M. Hoffman ; Protein activation dynamics in cells and tumor micro arrays assessed by time resolved Förster resonance energy transfer / Véronique Calleja, Pierre Leboucher, Banafshe ́Larijani ; Imaging stem cell differentiation for cell-based tissue repair / Zhenghong Lee ... [et al.] ; Understanding the initiation of B cell signaling through live cell imaging / Angel M. Davey ... [et al.] ; A quantitative method for measuring phototoxicity of a live cell imaging microscope / Jean-Yves Tinevez ... [et al.] ; High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines / Davide Danovi ... [et al.] ; High-throughput screening in primary neurons / Punita Sharma ... [et al.] ; Live imaging fluorescent proteins in early mouse embryos / Panagiotis Xenopoulos, Sonja Nowotschin, and Anna-Katerina Hadjantonakis ; Methods for three-dimensional analysis of dendritic spine dynamics / Enni Bertling ... [et al.] ; Imaging cell competition in Drosophila imaginal discs / Shizue Ohsawa ... [et al.] ; Live cell imaging of the oviduct / Sabine Kölle -- Computational quantification of fluorescent leukocyte numbers in zebrafish embryos / Felix Ellett and Graham J. Lieschke -- Four-dimensional tracking of lymphocyte migration and interactions in lymph nodes by two-photon microscopy / Masahiro Kitano and Takaharu Okada.
  • 2012 ScienceDirect
    Conn, P. Michael.
    Monitoring cytoskeletal dynamics in living neurons using fluorescence photoactivation / Anne Gauthier-Kemper ... [et al.] -- Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays / Roy Edward -- Methods to measure actin treadmilling rate in dendritic spines / Mikko Koskinen, Enni Bertling, and Pirta Hotulainen -- Live-cell imaging of clathrin coats / Comert Kural and Tom Kirchhausen -- Imaging the mitotic spindle / Paul S. Maddox ... [et al.] -- Modeling of spatial intracellular signaling events in neurons / Wendy C. Wenderski and Susana R. Neves -- Imaging chromosome dynamics in meiosis in plants / Moira J. Sheehan and Wojciech P. Pawlowski -- Analyzing lysosomes in live cells / Paul R. Pryor -- Detection and quantification of biomolecular association in living cells using single-molecule microscopy / Mario Brameshuber and Gerhard J. Schütz -- Visualizing cardiac ion channel trafficking pathways / James W. Smyth and Robin M. Shaw -- Live cell imaging of the cytoskeleton / Eve G. Stringham ... [et al.] -- Fluorescence single-molecule imaging of actin turnover and regulatory mechanisms / Naoki Watanabe -- Analysis of cell dispersion and migration by video-microscopy / Bonnomet Arnaud ... [et al.] -- Real time qualitative and quantitative GLUT4 translocation assay / Maleppillil Vavachan Vijayakumar and Manoj Kumar Bhat -- Advanced imaging of cellular signaling events / Marek Cebecauer, Jana Humpolíčková, and Jerémie Rossy -- Dynamic imaging of homo-FRET in live cells by fluorescence anisotropy microscopy / Subhasri Ghosh ... [et al.] -- Time-resolved luminescence resonance energy transfer imaging of protein-protein interactions in living cells / Harsha E. Rajapakse and Lawrence W. Miller -- Imaging of corticosteroid receptors in live cells / Mayumi Nishi -- Investigating second messenger signaling in vivo / Rüdiger Rudolf, Mathias Hafner, and Marco Mongillo -- Probes for intracellular RNA imaging in live cells / Philip J. Santangelo ... [et al.] -- Imaging the glycome in living systems / Boyangzi Li, Feiyan Mock, and Peng Wu -- Intracellular magnesium detection by fluorescent Indicators / Valentina Trapani ... [et al.] -- Illuminating mobile zinc with fluorescence : from cuvettes to live cells and tissues / Zhen Huang and Stephen J. Lippard -- Quantitative fluorescent live cell Imaging of intracellular Ca2+ and H+ ions in malaria parasites / Petra Rohrbach.
  • 2012 ScienceDirect
    Conn, P. Michael.
    Laser-induced radiation microbeam technology and simultaneous real-time fluorescence imaging in live cells / Stanley W. Botchway ... [et al.] -- A cell biologist's guide to high resolution imaging / Graeme Ball ... [et al.] -- Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo / André E.P. Bastos ... [et al.] -- Detecting and tracking nonfluorescent nanoparticle probes in live cells / Gufeng Wang and Ning Fang -- Fluorescence lifetime microscopy of tumor cell invasion, drug delivery, and cytotoxicity / Gert-Jan Bakker ... [et al.] -- Measuring membrane protein dynamics in neurons using fluorescence recovery after photobleach / Inmaculada M. González-González ... [et al.] -- Fluorescent speckle microscopy in cultured cells / Marin Barisic, António J. Pereira, and Helder Maiato -- Green-to-red photoconvertible mEosFP-aided live imaging in plants / Jaideep Mathur ... [et al.] -- Methods for cell and particle tracking / Erik Meijering, Oleh Dzyubachyk, and Ihor Smal -- Correlative light-electron microscopy : a potent tool for the imaging of rare or unique cellular and tissue events and structures / Alexander A. Mironov and Galina V. Beznoussenko -- Optical techniques for imaging membrane domains in live cells (live-cell palm of protein clustering) / Dylan M. Owen ... [et al.] -- Single live cell topography and activity imaging with the Shear-Force-Based Constant-Distance Scanning Electrochemical Microscope / Albert Schulte, Michaela Nebel, and Wolfgang Schuhmann -- Visualization of TGN-endosome trafficking in mammalian and drosophila cells / Satoshi Kametaka and Satoshi Waguri -- Live cell imaging with chemical specificity using dual frequency CARS microscopy / Iestyn Pope ... [et al.] -- Imaging intracellular protein dynamics by Spinning Disk Confocal microscopy / Samantha Stehbens ... [et al.] -- Visualizing dynamic activities of signaling enzymes using genetically encodable FRET-based biosensors : from designs to applications / Xin Zhou, Katie J. Herbst-Robinson, and Jin Zhang -- Live-cell imaging of Aquaporin-4 supramolecular assembly and diffusion / A.S. Verkman, Andrea Rossi, and Jonathan M. Crane -- Coiled-coil tag-probe labeling methods for live-cell imaging of membrane receptors / Yoshiaki Yano ... [et al.] -- Monitoring protein interactions in living cells with fluorescence lifetime imaging microscopy / Yuansheng Sun ... [et al.] -- Open source tools for fluorescent imaging / Nicholas A. Hamilton -- Nanoparticle PEBBLE sensors in live cells / Yong-Eun Koo Lee and Raoul Kopelman.
  • 2010 Springer
    Burry, Richard W.
  • 2010 Springer
    Buchwalow, Igor B.; Böcker, W.
  • 2011
    Meyer, Carissa Bove; Pfeffer, Suzanne R.; Straight, Aaron; Theriot, Julie A.
    Eukaryotic chromosomes segregate by attaching to microtubules of the mitotic spindle through a chromosomal microtubule-binding site called the kinetochore. Kinetochores assemble on a specialized chromosomal locus termed the centromere, characterized by the replacement of histone H3 in centromeric nucleosomes with the essential histone H3 variant centromere protein A (CENP-A). CENP-A nucleosomes are believed to epigenetically specify centromere identity, thus understanding how CENP-A chromatin is assembled and maintained is central to understanding chromosome segregation mechanisms. CENP-A nucleosome assembly requires the Mis18 complex and the CENP-A chaperone HJURP. HJURP binds to pre-nucleosomal CENP-A and facilitates the deposition of new CENP-A nucleosomes into centromeric chromatin. The mechanistic roles of the Mis18 complex in CENP-A assembly and maintenance are not well understood. The Mis18 complex and HJURP localize to centromeres in telophase/G1, when new CENP-A chromatin is assembled. The molecular interactions that control their targeting are unknown. Several constitutive centromere proteins that remain associated with CENP-A chromatin throughout the cell cycle have also been implicated in CENP-A assembly, including CENP-C. Their functions in CENP-A assembly are unknown. The first chapter of this thesis work describes the development of an in vitro system for centromeric chromatin assembly in Xenopus laevis egg extracts. We show that CENP-A assembly in extract recapitulates the cell cycle dependence and HJURP requirement of CENP-A assembly in somatic cells. We then use this in vitro system to identify a novel role for CENP-C in recruiting CENP-A assembly factors to the centromere to promote CENP-A assembly, described in Chapter 2. We show that CENP-C is required to target the Mis18 complex protein M18BP1 to Xenopus sperm centromeres in metaphase. In the absence of CENP-C, M18BP1 and HJURP targeting to centromeres is disrupted and new CENP-A assembly into centromeric chromatin is inhibited. We find that CENP-C interacts directly with M18BP1 through conserved domains in the CENP-C protein. Thus, CENP-C provides a link between existing CENP-A chromatin and the proteins required for new CENP-A nucleosome assembly. Overall, this work extends our mechanistic understanding of how the pre-existing centromere directs the local assembly of new CENP-A nucleosomes to ensure stable propagation of the centromere. Furthermore, the ability to assemble centromeric chromatin in vitro will provide a valuable tool for dissecting the biochemical and cell cycle regulatory mechanisms that control new CENP-A assembly and ensure faithful centromere propagation.
  • 2007 Springer Protocols
    Grandi, Guido.
    Energy systems for ATP regeneration in cell-free protein synthesis reactions / Kara A. Calhoun and James R. Swartz -- Continuous-exchange protein-synthesizing systems / Vladimir A. Shirokov ... [et al.] -- Cell-free production of integral membrane proteins on a preparative scale / Christian Klammt ... [et al.] -- SIMPLEX : a novel method for high-throughput construction and screening of protein libraries / Suang Rungpragayphan, Tsuneo Yamane, and Hideo Nakano -- Methods for high-throughput materialization of genetic information based on the wheat germ cell-free expression system / Tatsuya Sawasaki ... [et al.] -- Exogenous protein expression in Xenopus oocytes : basic procedures / Elena Bossi ... [et al.] -- In vitro translation to study HIV protease activity / Zene Matsuda ... [et al.] -- The protein truncation test in mutation detection and molecular diagnosis / Oliver Hauss and Oliver Muller -- Creation of novel enantioselective lipases by SIMPLEX / Yuichi Koga, Tsuneo Yamane, and Hideo Nakano -- In vitro transcription and translation coupled with two-dimensional electrophoresis for bacterial proteome analysis / Nathalie Norais ... [et al.] -- In vitro screen of bioinformatically selected Bacillus anthracis vaccine candidates by coupled transcription, translation, and immunoprecipitation analysis / Orit Gat, Haim Grosfeld, and Avigdor Shafferman -- Functional expression of Type 1 rat GABA transporter in microinjected Xenopus laevis oocytes / Stefano Giovannardi ... [et al.] -- Production of protein for nuclear magnetic resonance study using the wheat germ cell-free system / Toshiyuki Kohno and Yaeta Endo.
  • 2003 ScienceDirect
    Aalbersberg, W. Y.
  • 2005 CRCnetBASE
    Srivastava, Sudhir.
    Chapter 1. The Promise of Proteomics: Biology, Applications, and Challenges / Paul D. Wagner and Sudhir Srivastava -- Chapter 2. Proteomics Technologies and Bioinformatics / Sudhir Srivastava and Mukesh Verma -- Chapter 3. Creating a National Virtual Knowledge Environment.
  • 2007 CRCnetBASE
    Smith, Paul J.; Yue, Eddy W.
  • 2005 Springer
    Alexander, Denis; Cohen, Patricia T. W.; Pinna, Lorenzo A.
  • 2009 Springer
    Şener, Bilge.
  • 2009 Springer Protocols
    Barker, Christopher J.
    The role of inositol and the principles of labelling, extraction, and analysis of inositides in mammalian cells / Christopher J. Barker and Per-Olof Berggren -- HPLC separation of inositol polyphosphates / Christopher J. Barker, Christopher Illies, and Per-Olof Berggren -- Molecular manipulation and analysis of inositol phosphate and pyrophosphate levels in mammalian cells / James C. Otto and John D. York -- A femtomole-sensitivity mass assay for inositol hexakisphosphate / Andrew J. Letcher, Michael J. Schell, and Robin F. Irvine -- Synthesis of InsP₇ by the inositol hexakisphosphate kinase 1 (IP6K1) / Cristina Azevedo ... [et al.] -- Protein pyrophosphorylation by diphosphoinositol pentakisphosphate (InsP₇) / J. Kent Werner Jr., Traci Speed, and Rashna Bhandari -- Synthesis and nonradioactive micro-analysis of diphosphoinositol phosphates by HPLC with postcolumn complexometry / Hongying Lin, Karsten Lindner, and Georg W. Mayr -- Diphosphosinositol polyphosphates and energy metabolism : assay for ATP/ADP ratio / Andreas Nagel ... [et al.] -- Isolation of inositol 1,4,5-triphosphate receptor-associating proteins and selective knockdown using RNA interference / Akihiro Mizutani ... [et al.] -- Phosphoinositide-specific phospholipase C [beta]1 signal transduction in the nucleus / Roberta Fiume ... [et al.] -- Methods to assess changes in the pattern of nuclear phosphoinositides / Nullin Divecha -- Measurement of phosphoinositide 3-kinase products in cultured mammalian cells by HPLC / Frank T. Cooke -- Phosphoinositide analysis by liquid chromatography-mass spectrometry / Trevor R. Pettitt -- Imaging phosphoinositide dynamics in living cells / Anne Wuttke, Olof Idevall-Hagren, and Anders Tengholm.
  • 2003. ScienceDirect
    Kuksis, Arnis.
    Also available: Print – 2003
  • 2010 Springer
    Thany, Steeve Herve.
    Identification of cholinergic synaptic transmission in the insect nervous system / Steeve Hervé Thany, Hélène Tricoire-Leignel, and Bruno Lapied -- The evolution of pentameric ligand-gated ion channels / Joseph A. Dent -- Diversity of insect nicotinic acetylcholine receptor subunits / Andrew K. Jones and David B. Sattelle -- Identification of critical elements determining toxins and insecticide affinity, ligand binding domains, and channel properties / Hélène Tricoire-Leignel and Steeve Hervé Thany -- Electrophysiological studies and pharmacological properties of insect native nicotinic acetylcholine receptors / Steeve Hervé Thany -- Characterisation of insect nicotinic acetylcholine receptors by heterologous expression / Neil S. Millar and Stuart J. Lansdell -- Neonicotinoid insecticides : historical evolution and resistance mechanisms / Steeve Hervé Thany -- Ecotoxicity of neonicotinoid insecticides to bees / Axel Decourtye and James Devillers -- State of the art on insect nicotinic acetylcholine receptor function in learning and memory / Monique Gauthier.
    Also available: Print – 2010
  • 2015 Springer Protocols
    García-Fruitós, Elena.
    General introduction : recombinant protein production and purification of insoluble proteins / Neus Ferrer-Miralles ... [et al.] -- Overcoming the solubility problem in E. coli : available approaches for recombinant protein production / Agustín Correa and Pablo Oppezzo -- Optimization of culture parameters and novel strategies to improve protein solubility / Ranjana Arya ... [et al.] -- Cleavable self-aggregating tags (cSAT) for protein expression and purification / Zhanglin Lin ... [et al.] -- Beyond the cytoplasm of Escherichia coli : localizing recombinant proteins where you want them / Jason T. Boock ... [et al.] -- Characterization of amyloid-like properties in bacterial intracellular aggregates / Anna Villar-Pique, Susanna Navarro, and Salvador Ventura -- Co-translational stabilization of insoluble proteins in cell- free expression systems / Lei Kai ... [et al.] -- Functional expression of plant membrane proteins in Lactococcus lactis / Sylvain Boutigny .... [et al.] -- High cell-density expression system : yeast cells in a phalanx efficiently produce a certain range of "Difficult-to-Express" secretory recombinant proteins / Yasuaki Kawarasaki, Takeshi Kurose, and Keisuke Ito -- Insect cells-baculovirus system for the production of difficult to express proteins / Judit Osz-Papai ... [et al.] -- Transient expression in HEK 293 cells : an alternative to E. coli for the production of secreted and intracellular mammalian proteins / Joanne E. Nettleship ... [et al.] -- Recombinant glycoprotein production in human cell lines / Kamilla Swiech ... [et al.] -- Soluble recombinant protein production in Pseudoalteromonas haloplanktis TAC125 / Maria Giuliani ... [et al.] -- Screening methodology for purifying proteins with aggregation problems / Mario Lebendiker, Michal Maes, and Assaf Friedler -- Solubilization and refolding of inclusion body proteins / Anupam Singh, Vaibhav Upadhyay, and Amulya K. Panda -- Bacterial inclusion body purification / Joaquin Seras-Franzoso ... [et al.] -- Characterization of intracellular aggresomes by fluorescent microscopy / Lianwu Fu and Elizabeth Sztul -- Dialysis : a characterization method of aggregation tendency / Mireia Pesarrodona, Ugutz Unzueta, and Esther Vázquez -- Applications of mass spectrometry to the study of protein aggregation / Sílvia Bronsoms and Sebastián A. Trejo -- Insoluble protein assemblies characterized by Fourier transform infrared spectroscopy / Antonino Natalello and Silvia M. Doglia -- Insoluble protein characterization by circular dichroism (CD) spectroscopy and nuclear magnetic resonance (NMR) / Shaveta Goyal ... [et al.] -- Methods for characterization of protein aggregates / Witold Tatkiewicz ... [et al.] -- Predicting the solubility of recombinant proteins in Escherichia coli / Roger G. Harrison and Miguel J. Bagajewicz -- Insoluble protein applications : the use of bacterial inclusion bodies as biocatalysts / Eva Hrabárová , Lucia Achbergerová , and Jozef Nahálka.
  • 2006 ScienceDirect
    Romeo, John T.
    Terpene indole alkaloid biosynthesis / Sarah E. O'Connor, Elizabeth McCoy -- Pathways to diverse ergot alkaloid profiles in fungi / Daniel G. Panaccione, Christopher L. Schardl, Christine M. Coyle -- Compartmentalization of plant secondary metabolism / Nailish Samanani, Peter J. Facchini -- Comparative genomics of the shikimate pathways in Arabidopsis, Populus trichocarpa and Oryza sativa : shikimate pathway gene family structure and identification of candidates for missing links in pehnylalanine biosynthesis / Björn Hamberger ... [et al.] -- Tomato glandular trichomes as a model system for exploring evolution of specialized metabolism in a single cell / Eyal Fridman ... [et al.] -- Anacardic acid biosynthesis and bioactivity / David J. Schultz, Nalinie S. Wickramasinghe, Carolyn M. Klinge -- Molecular and biochemical investigations of sorgoleone biosynthesis / Daniel Cook ... [et al.] -- Biosynthesis of terpenophenolic metabolites in hop and cannabis / Jonathan E. Page, Jana Nagel -- Engineering pathway enzymes to understand the function and evolution of sterol structure and activity / Pruthvi Jayasimha ... [et al.] -- Methylation and demethylation of plant signaling molecules / Yue Yang ... [et al.] -- Recent advances in auxin biosynthesis and conjugation / Amber Kei Bowers, Yunde Zhao -- Auxin biology and biosynthesis / Jessica Calio, Yuen Yee Tam, Jennifer Normanly -- Translational opportunities in plant biochemistry / Cecilia A. McIntosh.
  • 2007 ScienceDirect
    Cheresh, David A.
    Also available: Print – 2007
  • 2006 Springer
    Paramio, Jesús M.
    Desmin and other intermediate filaments in normal and diseased muscle / Denise Paulin and Zhigang Xue -- Intermediate filaments in astrocytes in health and disease / Milos Pekny and Ulrika Wilhelmsson -- Neuronal intermediate filaments and neurodegenerative diseases / Gee Y. Ching and Ronald K.H. Liem -- Neurofilaments: phosphorylation and signal transduction / Sashi Kesavapany, Richard H. Quarles and Harish C. Pant -- Keratin intermediate filaments and diseases of the skin / E. Birgitte Lane -- The keratin K6 minifamily of genes / Manuel Navarro -- Transcriptional regulation of keratin gene expression / Miroslav Blumenberg -- Simple epithelial keratins: expression, function and disease / M. Llanos Casanova, Ana Bravo and José L. Jorcano -- Keratins as targets in and modulators of liver diseases / Kurt Zatloukal ... [et al.] -- The search for specific keratin functions: the case of keratin K10 / Mirentxu Santos ... [et al.].
  • 2007 Springer
    Hooper, Nigel M.; Lendeckel, Uwe.
    Site-2 protease at ten -- Signal peptide peptidases -- GXGD-type intramembrane proteases -- Rhomboid intramembrane serine proteases -- Proteases of the Rhomboid family in the yeast Saccharomyces cerevisiae -- [gamma]-Secretase and Alzheimer's disease -- [gamma]-Secretase mediated proteolysis: at the cutting edge of notch signaling.
  • 2015 Springer
    Felli, Isabella C.; Pierattelli, Roberta.
    1. Back to the future: nuclear magnetic resonance and bioinformatics studies on intrinsically disordered proteins -- 2. Structure and dynamics of intrinsically disordered proteins -- 3. NMR methods for the study of intrinsically disordered proteins structure, dynamics, and interactions: general overview and practical guidelines -- 4. Ensemble calculation for intrinsically disordered proteins using NMR parameters -- 5. NMR spectroscopic studies of the conformational ensembles of intrinsically disordered proteins -- 6. Recombinant intrinsically disordered proteins for NMR: tips and tricks -- 7. Biophysical methods to investigate intrinsically disordered proteins: avoiding an "elephant and blind men" situation -- 8. Application of SAXS for the structural characterization of IDPs -- 9. Bioinformatics approaches for predicting disordered protein motifs -- 10. Towards understanding protein disorder in-cell -- 11. The Protein Ensemble Database -- 12. Order and disorder in the replicative complex of paramyxoviruses -- 13. Druggability of intrinsically disordered proteins -- 14. Beta amyloid hallmarks: from intrinsically disordered proteins to Alzheimer's Disease.
    Also available: Print – 2015
  • 2007 Springer
    Leach, Andrew R.; Gillet, Valerie J.
  • 2002 Wiley
    Tsai, C. Stan.
    ch. 1. Introduction, p. 1-10 -- ch. 2. Biochemical Data: Analysis and Management, p. 11-40 -- ch. 3. Biochemical Exploration: Internet Resources, p. 41-52 -- ch. 4. Molecular Graphics: Visualization of Biomolecules, p. 53-71 -- ch. 5. Biochemical Compounds: Structure and Analysis, p. 73-105 -- ch. 6. Dynamic Biochemistry: Biomolecular Interactions, p. 107-121 -- ch. 7. Dynamic Biochemistry: Enzyme Kinetics, p. 123-146 -- ch. 8. Dynamic Biochemistry: Metabolic Simulation, p. 147-163 -- ch. 9. Genomics: Nucleotide Sequences and Recombinant DNA, p. 165-182 -- ch. 10. Genomics: Gene Identification, p. 183-208 -- ch. 11. Proteomics: Protein Sequence Analysis, p. 209-231 -- ch. 12. Proteomics: Prediction of Protein Structures, p. 233-268 -- ch. 13. Phylogenetic Analysis, p. 269-284 -- ch. 14. Molecular Modeling: Molecular Mechanics, p. 285-314 -- ch. 15. Molecular Modeling: Protein Modeling, p. 315-341 -- Appendices.
  • 2010 Wiley
    Mishra, Nawin C.
    "Proteomics provides an introductory insight on proteomics, discussing the basic principles of the field, how to apply specific technologies and instrumentation, and example applications in human health and diseases. With helpful study questions, this textbook presents an easy to grasp and solid overview and understanding of the principles, guidelines, and especially the complex instrumentation operations in proteomics for new students and research scientists. Written by a leader in proteomics studies, Proteomics offers an expert perspective on the field and the future of proteomics"--Provided by publisher.
    Also available: Print – v. [52], 2010.
  • 2003 CRCnetBASE
    Hatti-Kaul, Rajni; Mattiasson, Bo.
  • 2012 Springer
    Atreya, Hanudatta S.
    Also available: Print – 2012
  • 2015 ScienceDirect
    Kelman, Zvi.
    Isotope Labeling of Biomolecules - Labeling Methods, the latest volume of the Methods in Enzymology series contains comprehensive information on stable isotope labeling methods and applications for biomolecules.
  • 2012 Springer
    Decker, Thomas; Müller, Mathias.
    The Continuing Fascination with Jaks and Stats: An Introduction / Thomas Decker and Mathias Müller -- Regulation of JAKs: Insights Gleaned from the Functional Protein Domains / Claude Haan, Daniela Ungureanu, Tuija Pekkala, Olli Silvennoinen and Serge Haan -- Nuclear Functions of the Janus Kinases / Mark A. Dawson and Andrew J. Bannister -- Self-association of STAT Proteins from Monomers to Paracrystals / Mathias Droescher and Uwe Vinkemeier -- How Stats Interact with the Molecular Machinery of Transcriptional Activation / Amanda M. Jamieson, Matthias Farlik and Thomas Decker -- Nongenomic Functions of STAT3 / Daniel J. Gough, Pravin Sehgal and David E. Levy -- Evolution of Jak and Stat Proteins / Claus Vogl, Priyank Shukla and Ingo Ebersberger -- JAK/STAT and Chromatin Regulation in Drosophila / Kriti Gaur and Willis X. Li -- JAK/STAT Signaling and Invertebrate Immune Responses / Feng Zhou and Hervé Agaisse -- Stat5 as a Hematopoietic Master Regulator for Differentiation and Neoplasia Development / Harini Nivarthi, Katrin Friedbichler and Richard Moriggl -- Regulation of Dendritic Cell Development by STATs / Haiyan S. Li and Stephanie S. Watowich. STAT Transcription Factors: Controlling All Aspects of NK Cell Biology / Eva Maria Putz, Eva Zebedin and Veronika Sexl -- Function of JAKs and STATs in Lymphocytes: Bench to Bedside / Alejandro Villarino, Xiang-Ping Yang, Kiyoshi Hirahara, Yuka Kanno and John J. O' Shea, et al. -- The Role of Janus Kinases in Hematopoietic Malignancies / Damla Olcaydu and Robert Kralovics -- Activation of Janus Kinases During Tumorigenesis / Jeffrey W. Schmidt and Kay-Uwe Wagner -- STAT1 and STAT3 Transcription Factors in Inflammation-associated Colon Cancer / Paulina Rampetsreiter and Robert Eferl -- Universal and Specific Functions of STAT3 in Solid Tumours / Lidia Avalle, Gabriella Regis and Valeria Poli -- Impact of STAT5 on Normal Tissue Development and Cancer / Maria M. Caffarel and Christine J. Watson -- Crosstalk with the Jak-STAT Pathway in Inflammation / Lionel B. Ivashkiv -- Activation and Inhibition of JAK-STAT Signal Transduction by RNA Viruses / Curt M. Horvath -- Multifaceted Antiviral Actions of Interferon-stimulated Gene Products / Birgit Strobl, Nicole R. Leitner and Mathias Müller -- Interferon Regulation of the Innate Response to Bacteria / Ali Abdul-Sater and Christian Schindler.
  • 2011 Springer
    Rodríguez-Moreno, Antonio; Sihra, Talvinder S.
    "This volume critically examines the functional actions of the kainate type glutamate receptors (KARs). Following on from the larger body of work on the NMDA and AMPA-type ionotropic glutamate receptors (GluRs), studies with KARs have consistently thrown up exceptions to general rules about synaptic modulation. Contributors herein provide an insight to the idiosyncracies that now almost typify the KAR field. The fascinating insights provided in this volume serve to encourage searching mechanistic questions"--Provided by publisher.
    Also available: Print – 2011
  • 2016 Springer Protocols
    Goueli, Said A.; Zegzouti, Hicham.
    HTRF kinase assay development and methods in inhibitor characterization / Yong Jia, Mari Manuia, and Jose Juarez -- Application of eukaryotic elongation factor-2 kinase (eEF-2K) for cancer therapy : expression, purification, and high-throughput inhibitor screening / Clint D. J. Tavares ... [et al.] -- Recombinant kinase production and fragment screening by NMR spectroscopy / Byeonggu Han and Hee-Chul Ahn -- Bioluminescence methods for assaying kinases in quantitative high-throughput screening (qHTS) format applied to yes1 tyrosine kinase, glucokinase, and PI5P4Kα lipid kinase / Mindy I. Davis, Douglas S. Auld, and James Inglese -- Using bioluminescent kinase profiling strips to identify kinase inhibitor selectivity and promiscuity / Hicham Zegzouti, Jacquelyn Hennek, and Said A. Goueli -- Measuring activity of phosphoinositide lipid kinases using a bioluminescent ADP-detecting assay / Andrew W. Tai and Jolanta Vidugiriene -- High-throughput radiometric kinase assay / Krisna C. Duong-Ly and Jeffrey R. Peterson -- High-content assay to screen for modulators of EGFR function / Christophe Antczak and Hakim Djaballah -- Monitoring protein kinase expression and phosphorylation in cell lysates with antibody microarrays / Hong Zhang, Xiaoqing Shi, and Steven Pelech -- From enzyme to whole blood : sequential screening procedure for identification and evaluation of p38 MAPK inhibitors / Silke M. Bauer ... [e tal.] -- Genetically encoded fluorescent indicators to visualize protein phosphorylation in living cells / Moritoshi Sato and Yoshio Umezawa -- Characterization of an engineered Src kinase to study Src signaling and biology / Leanna R. Gentry .. [et al.] -- Screening one-bead-one-compound peptide libraries for optimal kinase substrates / Thi B. Trinh and Dehua Pei -- Determination of the substrate specificity of protein kinases with peptide micro- and macroarrays / Shenshen Lai ... [et al.] -- Rapid identification of protein kinase phosphorylation site motifs using combinatorial peptide libraries / Chad J. Miller and Benjamin E. Turk.
  • 2005 CRCnetBASE
    Jurisica, Igor; Wigle, Dennis.
  • 2013 ScienceDirect
    Lorsch, Jon.
    Section I. Cell protocols : basic microbiological techniques. Pouring agar plates and streaking or spreading to isolate individual colonies / Elizabeth Eyler ; Storage of bacteria and yeast / Cheryl M. Koh -- Section II. Cell protocols : cellular fractionation. Cellular fractionation : mammalian cells / Joerg von Hagen and Uwe Michelsen ; Cellular fractionation : yeast cells / Joerg von Hagen and Uwe Michelsen -- Section III. Cell protocols : Knock-outs and gene replacements. Yeast-gene replacement using PCR products / Megan Bergkessel, Christine Guthrie, and John Abelson ; Measuring RNAi knockdown using qPCR / Stuart Milstein, Michael Nguyen, Rachel Meyers, and Antonin de Fougerolles ; Recombineering : using drug cassettes to knock out genes in vivo / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court ; Gene knockouts, in vivo site-directed mutagenesis and other modifications using the delitto perfetto system in Saccharomyces cerevisiae / Samantha Stuckey and Francesca Storici ; Gene targeting and site-specific recombination in mouse ES cells / Konstantinos Anastassiadis, Frank Schnütgen, Harald von Melchner, and A. Francis Stewart ; Recombineering : highly efficient in vivo genetic engineering using single-strand oligos / James A. Sawitzke, Lynn C. Thomason, Mikhail Bubunenko, Xintian Li, Nina Costantino, and Donald L. Court -- Section IV. Cell protocols : microbiological media. Growth media for E. coli / Juliane C. Lessard ; Saccharomyces cerevisiae growth media / Jessica S. Dymond --Section V. Cell protocols : preparation of slides for microscopy. Immunohistochemistry on freely floating fixed tissue sections / Julia Bachman ; Preparation of slides for microscopy from frozen tissue sections / Julia Bachman ; Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry / Kirstie Canene-Adams ; Preparation of cells for microscopy using cytospin / Cheryl M. Koh ; Preparation of cells for microscopy using chamber slides and coverslips / Cheryl M. Koh ; Preparation of cells for microscopy using 'cell blocks' / Cheryl M. Koh -- Section VI. Lipid and carbohydrate protocols. Enzymatic deglycosylation of glycoproteins / Min-Sung Kim and Dan Leahy -- Section VII. Lipid and carbohydrate protocol : making vesicles and micelles. Preparation of fatty acid or phospholipid vesicles by thin-film rehydration / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder / Ting F. Zhu, Itay Budin, and Jack W. Szostak ; Preparation of fatty acid micelles / Ting F. Zhu, Itay Budin, and Jack W. Szostak -- Section VIII. Other protocols. Reverse-phase HPLC analysis and purification of small molecules / Kirstie Canene-Adams ; Thin layer chromatography / Marina Santiago and Scott Strobel ; ATP and GTP hydrolysis assays (TLC) / Vaishnavi Rajagopal and Jon R. Lorsch.
  • pt. A-D, 2014-15. ScienceDirect
    pt. B ScienceDirect
    pt. C ScienceDirect
    pt. D ScienceDirect
    Lorsch, Jon.
  • 2013 ScienceDirect
    Lorsch, Jon.
    Computational prediction of RNA secondary structure -- Reverse-transcription PCR (RT-PCR) -- Northern blotting -- Explanatory chapter: nuclease protection assays -- In vitro transcription from plasmid or PCR-amplified DNA -- Measurement of in vivo synthesis rates -- Method for measuring mRNA decay rate in saccharomyces cerevisiae -- Analysis of polysomes from bacteria -- Polysome profile analysis - yeast -- Polysome analysis of mammalian cells -- Polysome analysis for determining mRNA and ribosome association in saccharomyces cerevisiae -- Oligo(dT)-primed RT-PCR isolation of polyadenylated RNA degradation intermediates -- Circularized RT-PCR (cRT-PCR): analysis of the 5' ends, 3' ends, and poly(A) tails of RNA -- RNA radiolabeling -- Fluorescently labeling synthetic RNAs -- Analysis of RNA by analytical polyacrylamide gel -- RNA purification by preparative polyacrylamide gel -- Explanatory chapter: nucleic acid concentration determination -- RNA purification-precipitation methods -- Reverse transcriptase dideoxy sequencing of RNA -- RNA structure experimental analysis-chemical modification -- Structural analysis of RNA backbone using in-line probing.
  • 2012 Springer
    Takayama, Yoshiharu.
    "Lactoferrin was first identified as an iron-binding glycoprotein abundantly found in milk. It has been considered to play important roles in host defense, since lactoferrin shows broad spectrum of anti-bacterial and anti-viral activities, arising from its iron-chelating property. Furthermore, lactoferrin can regulate the function of innate and adaptive immune cells and exhibits immuno-modulating properties. These activities of lactoferrin implicate that potential use of lactoferrin as a wound healing agent. In addition, this book shows that lactoferrin is a potent regulator of dermal fibroblasts and epidermal keratinocytes, and promotes skin or corneal epithelial wound healing by increasing their proliferation, migration or deposition of extracellular matrix (ECM) components such as collagen and hyaluronan. This book provides the scientific background on use of lactoferrin as a wound healing agent. Indeed, lactoferrin exerts its biological effects by binding to specific lactoferrin receptors on target cells. This book also covers the in-depth information on roles of lactoferrin as a signaling molecule."--Publisher's website.
  • 2009 Springer
    Jeannin, Jean-François.
    Structure and synthesis of lipid A / Shoichi Kusumoto, Masahito Hashimoto and Kazuyoshi Kawahara -- Conformation and supramolecular structure of lipid A / Klaus Brandenburg and Ulrich Seydel -- Interactions between lipid A and serum proteins / Jèorg Andrèa ... [et al.] -- The lipid A receptor / Kiyoshi Takeda -- Lipid A receptor TLR4-mediated signaling pathways / Masahiro Yamamoto and Shizuo Akira -- Lipid A-induced responses in vivo / Néjia Sassi ... [et al.] -- Lipid A-mediated tolerance and cancer therapy / Cheryl E. Rockwell, David C. Morrison and Nilofer Qureshi -- Lipid A in cancer therapies : preclinical results / Daniele Reisser and Jean-François Jeannin -- Monophosphoryl lipid A (MPL) as an adjuvant for anti-cancer vaccines : clinical results / Christopher W. Cluff -- Antitumoral effects of lipids A : clinical studies / Marc Bardou and Danièle Reisser.
    Also available: Print – 2009
  • 2006 CRCnetBASE
    Mossoba, Magdi M.
    An overview of modern mass spectrometry methods in the toolbox of lipid chemists and biochemists / R. Moreau -- Techniques and applications in lipid analysis / Nils Hinrichsen and Hans Steinhart -- Recent advances in silver-ion HPLC utilizing acetonitrile in hexane as solvent: temperature effects on lipid elution orders/resolution / R.O. Adlof -- TLC-FID with special reference to marine lipids and other high-molecular-weight organic compounds / R.G. Ackman and A. Timmins -- Analysis of trans-18 :1 fatty acids by silver ion HPLC / P. Delmonte and M.P. Yurawecz -- Lipid separations using packed-column SFC / D.G. Hayes -- LC-MS and chiral separation / A. Kuksis -- LC-MS and lipid oxidation / A. Kuksis -- Structural analysis of unsaturated fatty acid methyl ester isomers with acetonitrile covalent adduct chemical ionization (CACI) / J.T. Brenna -- Fast GC for cellular fame analysis of bacteria / J.S. Buyer -- Use of cellular fatty acids to identify food-borne pathogens by infrared spectroscopy and capillary GC / M.M. Mossoba -- Infrared spectroscopy and partial least square calibration in the simultaneous quantification of isolated trans and conjugated linoleic acids / Alfred A. Christy -- Global cellular lipidome analyses by shotgun lipidomics using multidimensional mass spectrometry / X. Han and R.W. Gross -- Waxes and sterols: structures and chemistry / E.J. Parish and A.D. Bell -- Analysis of biological tissue and edible fat / J.K.G. Kramer -- Investigation of protein-lipid interactions by vibrational spectroscopy / E.C.Y. Li-Chan, G. Meng and N.K. Howell -- Fat replacers: an overview / W.E. Artz -- High-performance size-exclusion chromatography for lipid analysis in organic media / M.C. Dobarganes and G. Marquez-Ruiz -- Phospholipids : structures and physicochemical activities / M.C. Erickson.
  • 2014 Springer
    Kato, Yoji.
    This volume focusses on oxidative modifications of lipid molecules and the successive generation of singlet oxygen. The book also covers the secondary adductions of these reactive species with proteins and aminophospholipids. During lipid peroxidation, the initial event is the formation of lipid hydroperoxide, which is followed by an oxidation event that starts a chain-reaction. The formed lipid hydroperoxide gradually decomposes into harmful aldehydes, which are the advanced end-products of lipid peroxidation.
  • 2015 Springer
    Yassine, Hussein.
    This timely, concise title provides an important update on clinical lipid management. Using information from recent clinical trials and in special populations, the book begins by offering an easy-to-read overview of LDL, HDL, and triglyceride metabolism and the genetics of lipid disorders. The link between inflammation and lipids, and how this relates to atherosclerosis development, is also addressed, as are the measures of subclinical atherosclerosis in patients with abnormal lipid levels. Lipid abnormalities in children, with a particular focus on vulnerable populations (with an emphasis on ethnicity and childhood obesity), are covered. The treatment goals and approaches for managing lipids in the clinic are thoroughly discussed, emphasizing the important role of statin use and addressing controversies of lipid management in special populations such as heart failure, end stage kidney disease and fatty liver disease. Of special note, an important update on how new HIV medications impact lipid levels is provided. In all, Lipid Management: From Basics to Clinic, is an invaluable, handy resource for understanding changes in lipids in different populations and for sharpening the clinical approach to managing complicated lipid cases.
  • 2011 Springer
    Farooqui, Akhlaq A.
    "Lipid Mediators and Their Metabolism in the Brain presents readers with cutting edge and comprehensive information not only on the synthesis and degradation of glycerophospholipid-, sphingolipid-, and cholesterol-derived lipid mediators, but also their involvement in neurological disorders. It is hoped that this monograph will be useful not only to postgraduate student and their teachers, but also to research scientists and physicians, who are curious about the generation and roles of lipid mediators in the brain."--Publisher's website.
  • 2007 Springer Protocols
    McIntosh, Thomas J.
    Overview of membrane rafts / Thomas J. McIntosh -- Analysis of raft affinity of membrane proteins by detergent-insolubility / Deborah A. Brown -- Nondetergent isolation of rafts / Mehul B. Shah and Pravin B. Sehgal -- Detecting ordered domain formation (lipid rafts) in model membranes using tempo / Omar Bakht and Erwin London -- Using monomolecular films to characterize lipid lateral interactions / Rhoderick E. Brown and Howard L. Brockman -- Electro-formation and fluorescence microscopy of giant vesicles with coexisting liquid phases / Sarah L. Veatch -- Fluorescence correlation spectroscopy / Kristen Bacia and Petra Schwille -- Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells / Avery L. McIntosh ... [et al.] -- NMR detection of lipid domains / Ivan V. Polozov and Klaus Gawrisch -- Lateral diffusion coefficients of raft lipids from pulsed field gradient NMR / Greger Orädd and Göran Lindblom -- Saturation-recovery electron paramagnetic resonance discrimination by oxygen transport (DOT) method for characterizing membrane domains / Witold K. Subczynski ... [et al.] -- Plasmon-waveguide resonance spectoscopy studies of lateral segregation in solid-supported proteolipid bilayers / Zdzislaw Salamon, Savitha Devanathan, and Gordon Tollin -- Fluorescence recovery after photobleaching studies of lipid rafts / Anne K. Kenworthy -- Single-molecule tracking / Marija Vrljic, Stefanie Y. Nishimura, and W.E. Moerner -- X-ray diffraction to determine the thickness of raft and nonraft bilayers / Thomas J. McIntosh -- Small-angle neutron scattering to detect rafts and lipid domains / Jeremy Pencer ... [et al.] -- Exploring membrane domains using native membrane sheets and transmission electron microscopy / Bridget S. Wilson ... [et al.] -- Atomic force microscopy of lipid domains in supported model membranes / Alan R. Burns -- Atomistic and coarse-grained computer simulations of raft-like lipid mixtures / Sagar A. Pandit and H. Larry Scott -- A microscopic model calculation of the phase diagram of ternary mixtures of cholesterol and saturated and unsaturated phospholipids / R. Elliott, I. Szleifer, and M. Schick.
  • 2009 Springer Protocols
    Larijani, Banafshé; Rosser, Colin A.; Woscholski, Rudiger.
    Eicosanoids : generation and detection in mammalian cells / Valerie B. O'Donnell, Ben Maskrey, and Graham W. Taylor -- Lipidomic analysis of phospholipids and related structures by liquid chromatography-mass spectrometry / Trevor R. Pettitt -- Measurement of polyphosphoinositides in cultured mammalian cells / Frank T. Cooke -- Inositol lipid-dependent functions in Saccharomyces cerevisiae : analysis of phosphatidylinositol phosphates / Stephen K. Dove and Robert H. Michell -- Methods for the determination of the masss of nuclear PtdIns4P, PtdIns5P, and PtdIns(4,5)P₂ / David R. Jones ... [et al.] -- Lipid quantification and structure determination of nuclear envelope precursor membranes in the sea urchin / Marie Garnier-Lhomme ... [et al.] -- Solution and solid-state NMR of lipids / Axelle Grelard ... [et al.] -- Spatial localization of PtdInsP₂ in phase-separated giant unilamellar vesicles with a fluorescent PLC-delta 1 PH domain / Xavier Mulet ... [et al.] -- Studying cell-to-cell interactions : an easy method of tethering ligands on artificial membranes / Sebastian J. Fleire and Facundo D. Batista -- Ceramide-induced transbilayer (flip-flop) lipid movement in membranes / F.-Xabier Contreras ... [et al.] -- Effects of sterols on the development and aging of Caenorhabditis elegans / Eun-Young Lee ... [et al.] -- Proteomic analysis of the sterol-mediated signaling pathway in Caenorhabditis elegans / Byung-Kwon Choi ... [et al.] -- Sudying the subcellular localization and DNA-binding activity of FoxO transcription factors, downstream effectors of P13K/Akt / Abdelkader Essafi ... [et al.] -- Measurement of PTEN activity in vivo by imaging phosphorylated Akt / Erika Rosivatz and Rudiger Woscholski -- Analysis of phosphatidylinositol 3,4,5 trisphosphate 5-phosphatase activity by in vitro and in vivo assays / Lisa M. Ooms ... [et al.] -- Measuring phospholipase D activity in insulin-secreting pancreatic [beta]-cells and insulin-responsive muscle cells and adipocytes / Rosanna Cazzolli ... [et al.] -- Protein kinase C as an effector of lipid-derived second messengers / Marie-Hélène Paclet ... [et al.] -- Detection of myotubularin phosphatases activity on phosphoinositides in vitro and ex vivo / Holger Maria Rohde ... [et al.] -- Quantification of multiple phosphatidylinositol 4-kinase isozyme activities in cell extracts / Mark G. Waugh, Shane Minogue, and J. Justin Hsuan -- Phospholipid-interacting proteins by solution-state NMR spectroscopy / Keiichiro Kami, Sundaresan Rajesh, and Michael Overduin -- Revealing signaling in single cells by single- and two-photon fluorescence lifetime imaging microscopy / Damien Alcor, Veronique Calleja, and Banafshé Larijani -- Phosphoinositide (PI) 3-kinase assays / Michael J. Fry -- Measurement of phosphatidylinositol and phosphatidylcholine binding and transfer activity of the lipid transport Protein PITP / Shamshad Cockcroft -- In vitro reconstitution of activation of PLC[epsilon] by Ras and Rho GTPases / Natalia Lamuño Gandarillas ... [et al.] -- Assaying endogenous phosphatidylinositol-4-phosphate 5-kinase (PIP5K) activities / Jonathon R. Halstead ... [et al.] -- Preparation of membrane rafts / Mark G. Waugh and J. Justin Hsuan.
  • 2016 Springer Protocols
    Waugh, Mark.
    Method for assaying the lipid kinase phosphatidylinositol-5-phosphate 4-kinase α in quantitative high-throughput screening (qHTS) bioluminescent format / Mindy I. Davis, Atsuo T. Sasaki, and Anton Simeonov -- Assaying ceramide synthase activity in vitro and in living cells using liquid chromatography-mass spectrometry / Xin Ying Lim, Russell Pickford, and Anthony S. Don -- Fluorescent assays for ceramide synthase activity / Timothy A. Couttas and Anthony S. Don -- Identification of the interactome of a palmitoylated membrane protein, phosphatidylinositol 4-kinase type II alpha / Avanti Gokhale ... [et al.] -- Measurement of long-chain fatty Acyl-CoA synthetase activity / Joachim Füllekrug and Margarete Poppelreuther -- Qualitative and quantitative in vitro analysis of phosphatidylinositol phosphatase substrate specificity / Laura Ren Huey Ip and Christina Anja Gewinner -- Luciferase reporter assays to assess liver X receptor transcriptional activity / Matthew C. Gage, Benoit Pourcet, and Inés Pineda-Torra -- Metabolically biotinylated reporters for electron microscopic imaging of cytoplasmic membrane microdomains / Kimberly J. Krager and John G. Koland -- Fluorescence recovery after photobleaching analysis of the diffusional mobility of plasma membrane proteins : HER3 mobility in breast cancer cell membranes / Mitul Sarkar and John G. Koland -- Isolation and analysis of detergent-resistant membrane fractions / Massimo Aureli ... [et al.] -- Detection of isolated mitochondria-associated ER membranes using the sigma-1 receptor / Abasha Lewis, Shang-Yi Tsai, and Tsung-Ping Su -- Using surface plasmon resonance to quantitatively assess lipid-protein interactions / Kathryn Del Vecchio and Robert V. Stahelin -- Analyzing protein-phosphoinositide interactions with liposome flotation assays / Ricarda A. Busse ... [et al.] -- High-throughput fluorometric assay for membrane-protein interaction / Wonhwa Cho, Hyunjin Kim, and Yusi Hu -- Guidelines for the use of protein domains in acidic phospholipid imaging / Matthieu Pierre Platre and Yvon Jaillais -- Analysis of sphingolipid synthesis and transport by metabolic labeling of cultured cells with [3H]serine / Neale D. Ridgway -- Determination and characterization of tetraspanin-associated phosphoinositide-4 kinases in primary and neoplastic liver cells / Krista Rombouts and Vinicio Carloni -- Analysis of the phosphoinositide composition of subcellular membrane fractions / Deborah A. Sarkes and Lucia E. Rameh -- Single-molecule imaging of signal transduction via GPI- anchored receptors / Kenichi G. N. Suzuki -- Measuring phosphatidylinositol generation on biological membranes / Mark Waugh -- Assay for CDP-diacylglycerol generation by CDS in membrane fractions / Mark Waugh.
  • 2013 Springer
    Capelluto, Daniel G. S.
    Sphingomyelin and its role in cellular signaling / Mahua Chakraborty and Xian-Cheng Jiang -- The driving force of alpha-synuclein insertion and amyloid channel formation in the plasma membrane of neural cells: key role of ganglioside- and cholesterol-binding domains / Jacques Fantini and Nouara Yahi -- The enigmatic role of sulfatides: new insights into cellular functions and mechanisms of protein recognition / Shuyan Xiao, Carla V. Finkielstein, and Daniel G. S. Capelluto -- Phosphoinositides and PDZ domain scaffolds / Anna Maria Wawrzyniak, Rudra Kashyap and Pascale Zimmermann -- PtdIns(4)P signalling and recognition systems / Marc Lenoir and Michael Overduin -- PtdIns(4,5)P2-mediated cell signaling: emerging principles and PTEN as a paradigm for regulatory mechanism / Arne Gericke, Nicholas R. Leslie, Mathias Lösche, and Alonzo H. Ross -- Role of phosphatidylinositol 3,4,5-trisphosphate in cell signaling / Robert D Riehle, Sinziana Cornea and Alexei Degterev -- The role of phosphoinositides and inositol phosphates in plant cell signaling / Glenda E. Gillaspy -- Phosphatidic acid-mediated signaling / Yu Liu, Yuan Su, and Xuemin Wang -- Phosphatidylserine-mediated cellular signaling / Jason G. Kay and Sergio Grinstein -- Cardiolipin-mediated cellular signaling / Vinay A. Patil and Miriam L. Greenberg.
    Also available: Print – 2013
  • 2010 CRCnetBASE
    Murphy, Eric J.; Rosenberger, Thad A.
    Methods for measuring the activity and expression of phospholipases A2 / by Prerna Rastogi, Janhavi Sharma, and Jane McHowat -- Methods for measuring the activity and expression of phospholipase C / by Ryan A. Frieler, John A. Watt, and Thad A. Rosenberger -- Methods for measuring the activity and expression of phospholipase D / by Wenjuan Su and Michael A. Frohman -- Methods for measuring the activity and expression of diacylglycerol kinase / by Kaoru Goto ... [et al.] -- Methods for measuring endocannabinoid production and expression and activity of enzymes involved in the endocannabinoid system / by Tiziana Bisogno, Luciano De Petrocellis, and Vincenzo Di Marzo -- Mass spectrometry methods for the analysis of lipid molecular species: a shotgun lipidomics approach / by Xianlin Han ... [et al.] -- Mass spectrometry methods for the analysis of bioactive sphingolipids : a high-performance liquid-chromatography/tandem mass spectrometry approach / by Jacek Bielawski, Motohiro Tani, and Yusuf A. Hannun -- Mass spectrometry methods for the analysis of oxidant stress in biological fluids and tissues : quantification of F2-isoprostanes and F4-neuroprostanes using mass spectrometry / by Ginger L. Mine and Jason D. Morrow -- Methods for assessing the role of lipid-activated nuclear receptors in regulation of gene transcription / by Jenny Kaeding, Jocelyn Trottier, and Olivier Barbier -- Fluorescence methods to assess the impact of lipid binding proteins on ligand-mediated activation of gene expression / by Heather A. Hostetler ... [et al.] -- Methods for measuring fatty acids using chromatographic techniques / by Gwendolyn Barecló-Coblijn, Cameron C. Murphy, and Eric J. Murphy -- Methods for measuring fatty acid uptake and targeting in cultured cells and in vivo / by Paul G. Millner, Serena M. Lackman, and Eric J. Murphy -- Methods for kinetic analysis of fatty acid incorporation and turnover in vivo : a steady-state kinetic radiotracer approach / by Dhaval P. Bhatt and Thad A. Rosenberger.
  • 2007 ScienceDirect
    Brown, H. Alex.
    Also available: Print – 2007
  • 2007 ScienceDirect
    Brown, H. Alex.
    Also available: Print – 2007
  • 2007 ScienceDirect
    Brown, H. Alex.
    Also available: Print – 2007
  • 2013 Springer Protocols
    Kleinschmidt, Jörg H.
    Quartz crystal microbalances as tools for probing protein-membrane interactions / Søren B. Nielsen and Daniel E. Otzen -- Surface plasmon resonance for measuring interactions of proteins with lipid membranes / Vesna Hodnik and Gregor Anderluh -- Probing the thermodynamics of protein-lipid interactions by isothermal titration calorimetry / Musti J. Swamy and Rajeshwer S. Sankhala -- Differential scanning calorimetry of protein-lipid interactions / Olga Canadas and Cristina Casals -- Atomic force microscopy and spectroscopy to probe single membrane proteins in lipid bilayers / K. Tanuj Sapra -- Optimized negative-staining protocol for examining lipid-protein interactions by electron microscopy / Mark Garewal, Lei Zhang, and Gang Ren -- Examining protein-lipid complexes using neutron scattering / Luke A. Clifton, Cameron Neylon, and Jeremy H. Lakey -- Circular-dichroism and synchrotron-radiation circular-dichroism spectroscopy as tools to monitor protein structure in a lipid environment / Koichi Matsuo and Kunihiko Gekko -- Structural characterization of membrane proteins and peptides by FTIR and ATR-FTIR spectroscopy / Suren A. Tatulian -- Forster resonance energy transfer as a tool for quantification of protein-lipid selectivity / Luıs M.S. Loura, Manuel Prieto, and Fabio Fernandes -- Guide to tracking single transmembrane proteins in supported lipid bilayers / Kumud Raj Poudel, Jeffrey P. Jones, and James A. Brozik -- Fluorescence correlation spectroscopy to examine protein-lipid interactions in membranes / Viktoria Betaneli and Petra Schwille -- Analyzing transmembrane protein and hydrophobic helix topography by dual fluorescence quenching / Gregory A. Caputo and Erwin London -- Studying lipid-protein interactions with electron paramagnetic resonance spectroscopy of spin-labeled lipids / Tibor Pa li and Zoltan Kota -- EPR techniques to probe insertion and conformation of spin-labeled proteins in lipid bilayers / Enrica Bordignon and Yevhen Polyhach -- Solid-state NMR approaches to study protein structure and protein-lipid interactions / Christopher Aisenbrey [and others] -- Solution NMR spectroscopy for the determination of structures of membrane proteins in a lipid environment / Ashish Arora -- Nanodiscs as a New Tool to Examine Lipid-Protein Interactions / Mary A. Schuler, Ilia G. Denisov, and Stephen G. Sligar -- Simulation approach to lipid-protein interactions / Teresa Paramo [and others].
  • 2008 Springer
    Quinn, Peter J.; Wang, Xiaoyuan.
    Offers an account of the properties of the classes of lipids that are located in the membranes of various cells. This title presents a balanced perspective of what is known about the function of these lipids in healthy cells and tissues and what consequences follow malfunctions in their synthesis and metabolism.
  • 2015 Springer
    Gursky, Olga.
    Role of Lipids in Folding, Misfolding and Function of Integral Membrane Proteins -- Protein Misfolding in Lipid-Mimetic Environments.- Lipids in Amyloid-[beta] Processing, Aggregation, and Toxicity -- Role of Cholesterol and Phospholipids in Amylin Misfolding, Aggregation and Etiology of Islet Amyloidosis -- Stability, Oligomerization, and Amyloidogenicity of Apo Serum Amyloid A -- Interactions of Lipid Membranes with Fibrillar Protein Aggregates -- The Role of Lipid in Misfolding and Amyloid Fibril Formation by Apolipoprotein C-II -- Amyloid-Forming Properties of Human Apolipoproteins: Sequence Analyses and Structural Insights -- Computational Approaches to Identification of Aggregation Sites and the Mechanism of Amyloid Growth -- Role of Syndecans in Lipid Metabolism and Human Diseases
    Also available: Print – 2015
  • 2012 ScienceDirect
    Di Paolo, Gilbert; Wenk, Markus R.
    Supported native plasma membranes as platforms for the reconstitution and visualization of endocytic membrane budding / Min Wu, Pietro de Camilli -- Studying lipids involved in the endosomal pathway / Christin Bissig, Shem Johnson, Jean Gruenberg -- Studying in vitro membrane curvature recognition by proteins and its role in vesicular trafficking / Jean-Baptiste Manneville, Cécile Leduc, Benoit Sorre, Guillaume Drin -- Reconstituting multivesicular body biogenesis with purified components / Thomas Wollert -- Approaches to the study of Atg8-mediated membrane dynamics in vitro / Anjai Jotwani, Diana N. Richerson, Isabelle Motta, Omar Julca-Zevallos, Thomas J. Melia -- Reconstitution assay system for ceramide transport with semi-intact cells / Keigo Kumagai, Masahiro Nishijima, Kentaro Hanada -- Visualizing mitochondrial lipids and fusion events in mammalian cells / Huiyan Huang, Michael A. Frohman -- Targeted and non-targeted analysis of membrane lipids using mass spectrometry / Xue Li Guan, Markus R. Wenk -- Modulation of host phosphoinositide metabolism during salmonella invasion by the type III secreted effector SopB / Bernhard Roppenser, Sergio Grinstein, John H. Brumell -- Acute manipulation of phosphoinositide levels in cells / Belle Chang-Ileto, Samual G. Frere, Gilbert Di Paolo -- Regulation of phosphoinositide-metabolizing enzymes by clathrin coat proteins / Marnix Wieffer, Volker Haucke, Michael Krauß -- Phosphoinositides at the neuromuscular junction of drosophila melanogaster : a genetic approach / Jan R. Slabbaert, Thang Manh Khuong, Patrik Verstreken -- Devising powerful genetics, biochemical and structural tools in the functional analysis of phosphatidylinositol transfer proteins (PITPs) across diverse species / James M. Davison, Vytas A. Bankaitis, Ratna Ghosh -- Genome-wide screens for gene products regulating lipid droplet dynamics / Weihua Fei, Hongyuan Yang -- The three dimensionality of cell membranes : lamellar to cubic membrane transition as investigated by electron microsocopy / Ketpin Chong, Yuru Deng -- Quantitative imaging of lipid metabolism in yeast : from 4D analysis to high content screens of mutant libraries / Heimo Wolinski, Kristian Bredies, Sepp D. Kohlwein -- Analysis of cholesterol trafficking with fluorescent probes / Frederick R. Maxfield, Daniel Wüstner -- Fluorescence correlation methods for imaging cellular behavior of sphingolipid-interacting probes / Rachel Kraut, Nirmalya Bag, Thorsten Wohland -- Monitoring phospholipid dynamics during phagocytosis : application of genetically-encoded fluorescent probes / Helen Sarantis, Sergio Grinstein -- Genetically encoded probes for phosopatidic acid / Nawal Kassas, Petra Tryoen-Tóth, Matthias Corrotte, Tamou Thahouly, Marie-France Bader, Nancy J. Grant, Nicolas Vitale.
  • 2013 Springer Protocols
    Freeman, Lita A.
    The lipid-rich and otherwise challenging nature of many key tissues complicates many aspects of current research, and applications of the unique nature of lipoproteins and their biological effects has engendered unique and vital methodologies. In Lipoproteins and Cardiovascular Disease: Methods and Protocols, experts in the field present a compendium of advanced and classical molecular biology methods targeted towards lipoprotein, atherosclerosis, and vascular biology research, bringing together in a single volume an updated set of protocols and strategies for methods now driving the most recent advances, along with classical methods that are still widely used. Among the many topics covered in this cutting-edge work, the book delves into crucial techniques such as quantitative real-time PCR, microarrays, RT-PCR laser capture microdissection, and tissue-specific gene overexpression, knockout, and knockdown methodologies, including AAV as a liver-directed gene delivery vehicle. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and valuable notes which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Lipoproteins and Cardiovascular Disease: Methods and Protocols serves both novices and experts alike as a complete guide for any researcher with an interest in lipoproteins and their significant biological effects.
  • pt. A-G, 2003-09 ScienceDirect
    pt. B, 2003 ScienceDirect
    pt. C, 2003 ScienceDirect
    pt. D, 2004 ScienceDirect
    pt. E, 2005 ScienceDirect
    pt. F, 2009 ScienceDirect
    pt. G, 2009 ScienceDirect
    Düzgüneş, Nejat.
  • 2008
    Champe, Pamela C.; Ferrier, Denise R.; Harvey, Richard A.
    PrintStatus: Not Checked OutLane Catalog Record
    Unit I: Protein structure and function. Amino acids ; Structure of proteins ; Globular proteins ; Fibrous proteins ; Enzymes -- Unit II: Intermediary metabolism. Bioenergetics and oxidative phosphorylation ; Introduction to carbohydrates ; Glycolysis ; Tricarboxylic acid cycle ; Gluconeogenesis ; Glycogen metabolism ; Metabolism of monosaccharides and disaccharides ; Pentose phosphate pathway and NADPH ; Glycosaminoglycans and glycoproteins -- Unit III: Lipid metabolism. Metabolism of dietary lipids ; Fatty acid and triacylglycerol metabolism ; Complex lipid metabolism ; Cholesterol and steroid metabolism -- Unit IV: Nitrogen metabolism. Amino acids: disposal of nitrogen ; Amino acid degradation and synthesis ; Conversion of amino acids to specialized products ; Nucleotide metabolism -- Unit V: Integration of metabolism. Metabolic effects of insulin and glucagon ; The feed/fast cycle ; Diabetes mellitus ; Obesity ; Nutrition ; Vitamins -- Unit VI: Storage and expression of genetic information. DNA structure, replication and repair ; RNA structure, synthesis and processing ; Protein synthesis ; Regulation of gene expression ; Biotechnology and human disease.
  • 2011
    Harvey, Richard A.; Ferrier, Denise R.
    PrintStatus: Not Checked OutLane Catalog Record
    Protein structure and function -- Intermediary metabolism -- Lipid metabolism -- Nitrogen metabolism -- Integration of metabolism -- Storage and expression of genetic information.
  • 2003 Wiley
    Ardrey, R. E.
    ch. 1 Introduction, p. 1-5 -- ch. 2 Liquid Chromatography, p. 7-31 -- ch. 3 Mass Spectrometry, p. 33-74 -- ch. 4 Interface Technology, p. 75-127 -- ch. 5 Applications of High Performance Liquid Chromatography-Mass Spectrometry, p. 129-234.
  • 2011
    Lee, Peter Leader; Ferrell, James Ellsworth; Meyer, Tobias; Pfeffer, Suzanne R.; Theriot, Julie A.
    Membrane trafficking is an essential process that enables cells to deliver proteins to the extracellular space and segregate proteins into different compartments. Several steps are necessary for the functional segregation and processing of proteins. Trafficking includes post-translational modification of proteins, their movement between compartments, and stable localization once they have reached their target destinations. Importantly, membrane trafficking represents a recursively-sustained system: proteins that play a role in the regulation of membrane trafficking must also be properly transported and localized. Chapter one introduces the importance of the Golgi in the sorting and packaging of proteins and the roles of molecular motors in facilitating individual transport events. To modify transported cargoes, Golgi resident glycosyltransferases need to be localized to the correct cisternae and in some cases, in correct complex with other glycosyltransferases. "Kin recognition", the association of enzymatically compatible glycosyltransferases, has been demonstrated as a mechanism for the stable localization of glycosyltransferases to their proper compartments. Myosins, dyneins, and kinesins, linked to membranes by small Rab GTPases and their effectors, play important roles in moving cargo on actin and microtubule filaments. Their regulation by adapter proteins and their combinatorial interactions both complementing and opposing movement allows for the fine tuning of cargo transport. Chapter two presents the association of UDP-GlcNAc:[beta]Gal [beta]-1,3-N-acetylglucosaminyltransferase 1 (B3GNT1) and UDP-Gal:[beta]GlcNAc [beta]-1,4-galactosyltransferase, polypeptide 1 (B4GALT1). These two glycosyltransferases participate in the alternating, successive polymerization of poly-N-acetyllactosamine (polyLacNAc), a linear carbohydrate polymer composed of alternating N-acetylglucosamine and galactose residues. We show that these two glycosyltransferases colocalize by immunofluorescence microscopy, interact by coimmunoprecipitation, and when artificially retained in the ER can retain the complementary glycosyltransferase there as well. Chapter three presents the novel regulation of the KIF3 family kinesin, KIF1C, by small Rab GTPases, Rab1 and Rab6. The depletion of KIF1C impairs the plasma membrane delivery of Vesicular stomatitis virus G protein and intriguingly increases the speed of both Rab1 and Rab6 labeled vesicles. Rab6 can bind directly to the C-terminal domain of KIF1C and, like Rab1, can additionally interact with KIF1C's N-terminal motor domain. The interaction of KIF1C with Rab6 diminishes KIF1C's ability to bind microtubules. These results suggest that KIF1C acts as a brake, perhaps slowing transport to allow proper packaging and tethering to occur. Our data suggest that interaction between Rab6 and KIF1C could relieve KIF1C's braking action by displacing it from microtubules; alternatively, KIF1C may function as a molecular tether between membranes carrying Rab1 and/or Rab6.
  • 2013 Springer Protocols
    Bäckvall, Helena; Lehtiö, Janne.
    Part I. Low Molecular Weight Analysis Methods. Narrow-Range Peptide Isoelectric Focusing as Peptide Prefractionation Method Prior to Tandem Mass Spectrometry Analysis / Maria Pernemalm -- Analysis of Peptides by Denaturing Ultrafiltrationand LC-MALDI-TOF-MS / Yanming An and Radoslav Goldman -- Stable Isotope Labeling Methods in Protein Profiling / Johan Lengqvist and AnnSofi Sandberg -- Development of MRM-Based Assays for the Absolute Quantitation of Plasma Proteins / Michael A. Kuzyk, Carol E. Parker, Dominik Domanski, and Christoph H. Borchers -- Mass Spectrometric Profiling of Low-Molecular-Weight Proteins / Matthias Rainer, Constantin Sajdik, and Günther K. Bonn -- Developing an iMALDI Method / Brinda Shah, Jennifer D. Reid, Michael A. Kuzyk, Carol E. Parker, and Christoph H. Borchers -- Analysis of Neuropeptides by MALDI Imaging Mass Spectrometry / Anna Ljungdahl, Jörg Hanrieder, Jonas Bergquist, and Malin Andersson -- Highly Multiplexed Antibody Suspension Bead Arrays for Plasma Protein Profiling / Kimi Drobin, Peter Nilsson, and Jochen M. Schwenk -- Part II. Bioinformatics Of Proteomics Data. Protein Quantification by Peptide Quality Control (PQPQ) of Shotgun Proteomics Data / Jenny Forshed -- Part III Methods Applied In Clinical Research. Collection and Handling of Blood Specimens for Peptidomics / Harald Tammen and Rudiger Hess -- An Automated RP-SCX Solid-Phase Extraction Procedure for Urinary Peptidomics Biomarker Discovery Studies / Crina I.A. Balog, Rico Derks, Oleg A. Mayboroda, and André M. Deelder -- Application of Phage Display for Ligand Peptidomics to Identify Peptide Ligands Binding to AQP2-Expressing Membrane Fractions / Byung-Heon Lee and Tae-Hwan Kwon -- Protein Expression Profiling of Brain Tumor Tissue Using SELDI-MS / Carl Wibom -- Comprehensive Analysis of MHC Ligands in Clinical Material by Immunoaffinity-Mass Spectrometry / Kie Kasuga.
  • 2006 Springer
    Tsoukas, Constantine.
    Protein kinase C-theta (PKC[theta]): new perspectives on its functions in T-cell biology / Rina Barouch-Bentov and Amnon Altman -- Travel informations on the tec kinases during lymphocyte activation / Fabien Garçon and Jacques A. Nunès -- Inducible T-cell tyrosine kinase (ITK): structural requirements and actin polymerization / Constantine D. Tsoukas ... [et al.] -- Differential requirements of PI3K subunits for BCR or BCR/CD19-induced ERK activation / Elena Vigorito and Martin Turner -- Protein tyrosine phosphatases in human disease / Tomas Mustelin -- The T-cell-specific adapter protein functions as a regulator of peripheral but not central immunological tolerance / Philip E. Lapinski ... [et al.] -- Proximal signals controlling B-cell antigen receptor (BCR) mediated NF-[kappa]B activation / Miguel E. Moreno-García ... [et al.] -- The adapter 3BP2: how it plugs into leukocyte signaling / Marcel Deckert and Robert Rottapel -- CTLA-4 regulation of T-cell function via RAP-1-mediated adhesion / Helga Schneider ... [et al.] -- Protein crosstalk in lipid rafts / Raquel J. Nunes, Mónica A.A. Castro, and Alexandre M. Carmo -- Role of lipid rafts in activation-induced cell death: the fas pathway in aging / Anis Larbi ... [et al.] -- T-cell response in aging: influence of cellular cholesterol modulation / Tamàs Fülöp ... [et al.] -- Lymphocyte signaling and the translatability of mRNA / Suzanne Miyamoto -- Protein arginine methylation: a new frontier in T-cell signal transduction / Brandon T. Schurter, Fabien Blanchet, and Oreste Acuto -- Immune regulation by ubiquitin conjugation / K. Venuprasad ... [et al.] -- CTIB (C-terminus protein of I[kappa]B-[beta]): a novel factor required for acidic adaptation /Toshiko Fukamachi ... [et al.] -- HIV may deplete most CD4 lymphocytes by a mechanism involving signaling through its receptors on non-permissive resting lymphocytes / Miles W. Cloyd ... [et al.] -- Integrating traditional and postgenomic approaches to investigate lymphocyte development and function / Yina Hsing Huang ... [et al.] -- Phosphoproteomic analysis of lymphocyte signaling / Lulu Cao, Kebing Yu, and Arthur R. Salomon.
    Also available: Print – 2006
  • 2015 ScienceDirect
    Platt, Frances M.; Platt, Nick.
  • 2014 Springer
    Anderluh, Gregor; Gilbert, Robert.
    Introduction: brief historical overview -- Distribution of MACPF/CDC proteins -- Evolution of the complement system -- Structural features of cholesterol dependent cytolysins and comparison to other MACPF-domain containing proteins -- Perfringolysin O structure and mechanism of pore formation as a paradigm for cholesterol-dependent cytolysins -- Structural biology of the membrane attack complex -- Membrane interactions and cellular effects of MACPF/CDC proteins -- The biology of pneumolysin -- Multifaceted activity of listeriolysin O, the cholesterol -dependent cytolysin of Listeria monocytogenes -- Perforin: a key pore-forming protein for immune control of viruses and cancer -- Perforin and human diseases -- The role of MACPF proteins in the biology of malaria and other apicomplexan parasites -- Chlamydial MACPF protien CT153 -- Fungal MACPF-like proteins and aegerolysins: bi-component pore-forming proteins? -- Fluorescence imaging of MACPF/CDC proteins: new techniques and their application.
  • 2015 Springer Protocols
    Sun, Xue-Long.
    Synthesis of chain-end functionalized glycopolymers via cyanoxyl-mediated free radical polymerization (CMFRP) / Valentinas Gruzdys ... [et al.] -- Protecting-group-free synthesis of well-defined glycopolymers featuring negatively charged oligosaccharides / Luca Albertin -- Glycopolymers prepared by ring-opening metathesis polymerization followed by glycoconjugation using a triazole-forming "click" reaction / Ronald Okoth and Amit Basu -- Protecting-group-free synthesis of glycopolymers and their binding assay with lectin and influenza virus / Tomonari Tanaka, Tadanobu Takahashi, and Takashi Suzuki -- Carbohydrate-based initiators for the cationic ring-opening polymerization of 2-Ethyl-2-Oxazoline / Christine Weber ... [et al.] -- Heterofunctional glycopolypeptides by combination of Thiol-Ene chemistry and NCA polymerization / Kai-Steffen Krannig and Helmut Schlaad -- Preparation of proteoglycan mimetic graft copolymers / Matt J. Kipper and Laura W. Place -- Galactosylated polymer nano-objects by polymerization-induced self-assembly, potential drug nanocarriers / Mona Semsarilar, Irene Canton, and Vincent Ladmiral -- Synthetic approach to biotinylated glyco-functionalized quantum dots : a new fluorescent probes for biomedical applications / Christian K. Adokoh, James Darkwa, and Ravin Narain -- Surface modification of polydivinylbenzene microspheres with a fluorinated glycopolymer using Thiol-Halogen click chemistry / Wentao Song and Anthony M. Granville -- Glycopolymer-grafted polymer particles for lectin recognition / Michinari Kohri, Tatsuo Taniguchi, and Keiki Kishikawa -- Synthesis of non-spherical glycopolymer-decorated nanoparticles : combing Thiol-ene with catecholic chemistry / Xiao Li, Weidong Zhang, and Gaojian Chen -- Synthetic approach to glycopolymer base nanoparticle gold(I) conjugate : a new generation of therapeutic agents / Christian K. Adokoh, James Darkwa, and Ravin Narain -- Multivalent glycopolymer-coated gold nanoparticles / Sarah-Jane Richards ... [et al.] -- Modulation of multivalent protein binding on surfaces by glycopolymer brush chemistry / Kai Yu ... [et al.] -- Oriented immobilized sialyloligo-macroligand microarray / Satya Nandana Narla and Xue-Long Sun -- Glycocalyx remodeling with glycopolymer-based proteoglycan mimetics / Mia L. Huang ... [et al.].
  • 2012 Springer
    Carrondo, Maria Arménia; Spadon, Paola.
  • 2011
    Weber, Stephanie Crane; Kaiser, A. Dale; Shapiro, Lucy; Spakowitz, Andrew; Straight, Aaron; Theriot, Julie A.
    The cellular interior is crowded with macromolecules that form highly organized but dynamic structures. While these properties of the cytoplasm have become well known, their physical consequences on intracellular processes such as transport and gene expression are still not well understood. The goal of this dissertation is to begin to elucidate these physical consequences and to develop a new model for macromolecular motion in vivo. Tracking of fluorescently labeled chromosomal loci in live bacterial cells reveals a robust subdiffusive scaling of the mean square displacement (MSD). To identify the source of anomalous behavior, we developed analytical theory and Brownian dynamics simulations of polymers under various physical environments. Specifically, we examined the consequences of confinement, self-interaction, viscoelasticity and random waiting on monomer motion. We found that neither confinement nor self-interaction alter the fundamental Rouse mode relaxations of a polymer. However, viscoelasticity, modeled by fractional Langevin motion (fLm), and random waiting, modeled with a continuous time random walk (CTRW), led to significant and distinct deviations from the classic polymer dynamics model. We propose that the subdiffusive motion of chromosomal loci observed in vivo arises from relaxation of the Rouse modes of the DNA polymer within the viscoelastic environment of the cytoplasm. The time-averaged and ensemble-averaged MSD of chromosomal loci exhibit ergodicity, and the velocity autocorrelation function is negative at short time lags. These observations are most consistent with fLm and rule out a CTRW model as an explanation for anomalous motion in vivo. In contrast to the subdiffusive scaling exponent, the apparent diffusion coefficient is variable across species and sensitive to biological perturbations. When ATP synthesis is inhibited in E. coli, the apparent diffusion coefficient decreases 2-fold while the scaling exponent remains constant. This observation suggests that metabolic activity contributes to the magnitude of locus motion, but does not affect the subdiffusive mechanism (or the viscoelastic properties of the cytoplasm). Furthermore, the temperature-dependence of locus motion is steeper in untreated cells than in ATP-depleted cells. These results suggest that untreated cells have an additional source of molecular agitation that increases with temperature. Such ATP-dependent fluctuations are likely mechanical, as the heat dissipated from glycolysis and oxidative phosphorylation is insufficient to account for the difference in the apparent diffusion coefficient between untreated and ATP-depleted cells. We are currently investigating the dynamic contributions of several molecular candidates. Our data indicate that ATP-dependent enzymatic activity, in addition to thermal fluctuations, may contribute to the molecular agitation driving (sub)diffusion in the cell.
  • 2010 Springer Protocols
    Seger, Rony.
    The MAP kinase signaling cascades : A system of hundreds of components regulates a diverse array of physiological functions -- Determination of ERK activity: Anti-phospho-ERK antibodies and in vitro phosphorylation -- Activation of SAPK/JNKs in vitro -- Activation of p38 and determination of its activity -- Activity assays for extracellular signal-regulated kinase 5 -- Use of inhibitors in the study of MAP kinases -- MAP kinase activation by receptor tyrosine kinases : in control of cell migration -- Activation of ras and rho GTPases and MAP kinases by G-protein-coupled receptors -- Regulation of MAP kinase signaling by calcium -- Identification of novel substrates of MAP kinase cascades using bioengineered kinases that uniquely utilize analogs of ATP to phosphorylate substrates -- ERK-MAP kinase signaling in the cytoplasm -- Lentiviral vectors to study the differential function of ERK1 and ERK2 MAP kinases -- Structural studies of MAP kinase cascade components -- Analysis of MAP kinases by hydrogen exchange mass spectrometry -- A "Molecular evolution" approach for isolation of intrinsically active (MEK-independent) MAP kinases -- Reconstitution of the nuclear transport of the MAP kinase ERK2 -- Localization and trafficking of fluorescently tagged ERK1 and ERK2 -- Studying the regulation of MAP kinase by MAP kinase phosphatases in vitro and in cell systems -- Proteomic analysis of scaffold proteins in the ERK cascade -- Analysis of ERKs' dimerization by electrophoresis -- MAP kinase: SUMO pathway interactions -- Computational modelling of kinase signalling cascades -- Analysis of mitogen-activated protein kinase activity in yeast -- Detection of RTK pathway activation in drosophila using anti-dpERK immunofluorescence staining -- Studying MAP kinase pathways during early development of xenopus laevis -- Deciphering signaling pathways in vivo : The ras/raf/MEK/ERK cascade -- Mutational and functional analysis in human ras/MAP kinase genetic syndromes -- Implication of the ERK pathway on the post-transcriptional regulation of VEGF mRNA stability -- Studies on MAP kinase signaling in the immune system -- Methods to study MAP kinase signalling in the central nervous system -- MAP kinase regulation of the mitotic spindle checkpoint -- Using high-content microscopy to study gonadotrophin-releasing hormone regulation of ERK.
  • 2013 Springer Protocols
    Matthiesen, Rune.
    Since the publishing of the first edition, the methodologies and instrumentation involved in the field of mass spectrometry-based proteomics has improved considerably. Fully revised and expanded, Mass Spectrometry Data Analysis in Proteomics, Second Edition presents expert chapters on specific MS-based methods or data analysis strategies in proteomics. The volume covers data analysis topics relevant for quantitative proteomics, post translational modification, HX-MS, glycomics, and data exchange standards, among other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and authoritative, Mass Spectrometry Data Analysis in Proteomics, Second Edition serves as a detailed guide for all researchers seeking to further our knowledge in the field of proteomics.
  • 2006 Springer Protocols
    Matthiesen, Rune.
  • 2013 Springer Protocols
    Kohler, Jennifer; Patrie, Steven M.
    Introduction to glycosylation and mass spectrometry / Steven M. Patrie, Michael J. Roth, and Jennifer J. Kohler -- PART I ENRICHMENT AND ISOLATION METHODS -- Tandem lectin weak affinity chromatography for glycoprotein enrichment / Zhi Yuan Ma, Yuliya Skorobogatko, and Keith Vosseller -- CSC technology: Selective labeling of glycoproteins by mild oxidation to phenotype cells / Andreas Hofmann, Damaris Bausch-Fluck, and Bernd Wollscheid -- Use of boronic acid nanoparticles in glycoprotein enrichment / Yawei Xu, Lijuan Zhang, and Haojie Lu -- Incorporation of unnatural sugars for the identification of glycoproteins / Balyn W. Zaro, Howard C. Hang, and Matthew R. Pratt -- Characterization of membrane-associated glycoproteins using lectin affinity chromatography and mass spectrometry / Yashu Liu, Jintang He, and David M. Lubman -- Sialic acid capture-and-release and LC-MS(n) analysis of glycopeptides / Jonas Nilsson and Göran Larson -- PART II SAMPLE PREPARATION -- In-solution digestion of glycoproteins for glycopeptide-based mass analysis / Eden P. Go, Kathryn R. Rebecchi, and Heather Desaire -- Nano-HPLC-MS of glycopeptides obtained after nonspecific proteolysis / Gerhild Zauner, Carolien A.M. Koeleman, André M. Deelder, and Manfred Wuhrer -- PART III SEPARATION METHODS -- Glycopeptide enrichment for MALDI-TOF mass spectrometry analysis by hydrophilic interaction liquid chromatography solid phase extraction (HILIC SPE) / Pia Hønnerup Jensen, Simon Mysling, Peter Højrup, and Ole Nørregaard Jensen -- Separation and identification of glycoforms by capillary electrophoresis with electrospray ionization mass spectrometric detection / Alina D. Zamfir, Corina Flangea, Alina Serb, Ana-Maria Zagrean, Andreas M. Rizzi, and Eugen Sisu -- Structural separations by ion mobility-MS for glycomics and glycoproteomics / Larissa S. Fenn and John A. McLean -- PART IV QUANTITATION METHODS -- Quantitative analysis of glycoprotein glycans / Ron Orlando -- Stable isotope labeling of N-glycosylated peptides by enzymatic deglycosylation for mass spectrometry-based glycoproteomics / Hiroyuki Kaji and Toshiaki Isobe -- Approaches for site mapping and quantification of o-linked glycopeptides / Peng Zhao, Stephanie H. Stalnaker, and Lance Wells -- Glycan profiling: Label-free analysis of glycoproteins / Yoshinao Wada -- PART V COMPUTATIONAL TOOLS -- Introduction to informatics in glycoprotein analysis / Kiyoko F. Aoki-Kinoshita -- Software tools for glycan profiling / Chuan-Yih Yu, Anoop Mayampurath, and Haixu Tang -- PART VI CASE STUDIES IN MASS SPECTROMETRY OF GLYCOPROTEINS -- Quantitative characterization of glycoproteins in neurodegenerative disorders using iTRAQ / Min Shi, Hyejin Hwang, and Jing Zhang -- Quantitative proteomic analysis of N-linked glycoproteins in human tear fluid / Lei Zhou and Roger W. Beuerman -- Elucidation of N-glycosites within human plasma glycoproteins for cancer biomarker discovery / Penelope Drake, Birgit Schilling, Brad Gibson, and Susan Fisher -- Characterizing the glycosylation state of therapeutic recombinant glycoproteins. / Nicole Samuels, David Kates, Jun Liu, and Joanne Severs.

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