• pt. A-B, 2001.From: ScienceDirect
    pt. BFrom: ScienceDirect
    edited by Allen W. Nicholson.
    Also available: Print – pt. A-B, 2001.
  • 2012From: Springer Protocols
    edited by Julie A. Douthwaite and Ronald H. Jackson.
    Ribosome display : a perspective / Andreas Plückthun -- Preparation and testing of E. coli S30 in vitro transcirption translation extracts / James F. Zawada -- Eurkaryotic ribosome display selection using rabbit reticulocyte lysate / Julie A Douthwaite -- Stabilized ribosome display for in vitro selection / Shuta Hara [and others] -- Eukaryotic ribosome display with in situ DNA recovery / Mingyue He [and others] -- mRNA display using covalent coupling of mRNA to translated proteins / Rong Wang, Steve W. Cotten, and Rihe Liu -- SNAP display : in vitro rotein evolution in microdroplets / Miriam Kaltenbach and Florian Hollfelder -- cDNA display : rapid stabilization of mRNA display / Shingo Ueno and Naoto Nemoto -- Optimisation of antibody affinity by ribosome display using error-prone or site-directed mutagenesis / Leeanne Lewis and Chris Lloyd -- Affinity maturation of phage display antibody populations using ribosome display / Maria A. Groves and Adrian A. Nickson -- Evolution of protein stability using ribosome display / Andrew Buchanan -- Selection of lead antibodies from naive ribosome display antibody libraries / Peter Ravn -- Evolution of disulfide-rich peptide aptamers using cDNA display / Yuki Mochizuki and Naoto Nemoto -- Peptide screening using PURE ribosome display / Hiroyuki Ohashi [and others] -- Rapid selection of high-affinity binders using ribosome display / Birgit Dreier and Andreas Plückthun -- mRNA display-based selections using synthetic peptide and natural protein libraries / Steve W. Cotten [and others] -- Identification of candidate vaccine genes using ribosome display / Liancheng Lei -- Ribosome display for the selection of Sac7d scaffolds / Barbara Mouratou [and others] -- Charging of tRNAs using ribozymes and selection of cyclic peptides containing thioethers / Patrick C. Reid [and others] -- Update on pure translation display with unnatural amino acid incorporation / R. Edward Watts and Anthony C. Forster -- In vitro selection of unnatural cyclic peptide libraries via mRNA display / Zhong Ma and Matthew C.T. Hartman -- Optimization of CAT-354, a therapeutic antibody directed against interleukin-13, using ribosome display / George Thom and Ralph Minter -- Affinity maturation and functional dissection of a humanised anti-RAGE monoclonal antibody by ribosome display / Simon E. Hufton.
  • 1988From: ScienceDirect
    edited by Harry F. Noller, Jr., Kivie Moldave.
    Also available: Print – 1988
  • 2011From: Springer
    Marina V. Rodnina, Wolfgang Wintermeyer, Rachel Green, editors.
    The ribosome is a macromolecular machine that synthesizes proteins with a high degree of speed and accuracy. Our present understanding of its structure, function and dynamics is the result of six decades of research. This book collects over 40 articles based on the talks presented at the 2010 Ribosome Meeting, held in Orvieto, Italy, covering all facets of the structure and function of the ribosome. New high-resolution crystal structures of functional ribosome complexes and cryo-EM structures of translating ribosomes are presented, while partial reactions of translation are examined in structural and mechanistic detail, featuring translocation as a most dynamic process. Mechanisms of initiation, both in bacterial and eukaryotic systems, translation termination, and novel details of the functions of the respective factors are described. Structure and interactions of the nascent peptide within, and emerging from, the ribosomal peptide exit tunnel are addressed in several articles. Structural and single-molecule studies reveal a picture of the ribosome exhibiting the energy landscape of a processive Brownian machine. The collection provides up-to-date reviews which will serve as a source of essential information for years to come.
  • 2014From: ScienceDirect
    edited by Donald H. Burke-Aguero.
    This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch structure and function methods, folding pathways and dynamics, and ligand interactions and thermodynamics.Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in biomineralization scienceContains sections on such topics as riboswitch discovery and valid.
  • 2015From: ScienceDirect
    edited by Donald H. Burke-Agüero.
  • 2012From: Springer Protocols
    edited by Jörg S. Hartig.
    Introduction -- Characterization of hammerhead ribozyme reactions -- Mechanistic analysis of the hepatitis delta virus (HDV) ribozyme: Methods for RNA preparation, structure mapping, solvent isotope effects, and co-transcriptional cleavage -- Kinetic characterization of hairpin ribozyme variants -- Characterization of RNase p rna activity -- Group I intron ribozymes -- Kinetic characterization of group II intron folding and splicing -- Mechanism and distribution of glmS ribozymes -- Structure-based search and in vitro analysis of self-cleaving ribozymes -- Discovery of rna motifs using a computational pipeline that allows insertions in paired regions and filtering of candidate sequences -- Crystallographic analysis of small ribozymes and riboswitches -- Functional dynamics of RNA ribozymes studied by NMR spectroscopy -- Deoxyribozyme-based, semisynthetic access to stable peptidyl-trnas exemplified by tRNA(Val) carrying a macrolide antibiotic resistance peptide -- Probing functions of the ribosomal peptidyl transferase center by nucleotide analog interference -- Single molecule fret characterization of large ribozyme folding -- Metal ion-RNA interactions studied via multinuclear NMR -- Analysis of catalytic RNA structure and function by nucleotide analog interference mapping -- In vitro selection of metal ion-selective dnazymes -- Selecting allosteric ribozymes -- Screening effective target sites on mRNA: A ribozyme library approach -- A computational approach to predict suitable target sites for trans-acting minimal hammerhead ribozymes -- Targeting mRNAs by engineered sequence-specific RNase p ribozymes -- Target-induced SOFA-HDV ribozyme -- Ribozyme-mediated trans insertion-splicing into target RNAs -- Developing fluorogenic RNA-cleaving dnazymes for biosensing applications -- Development of trainable deoxyribozyme-based game playing automaton -- Rational design and tuning of ribozyme-based devices -- In vivo screening of ligand-dependent hammerhead ribozymes -- Flexizymes as a tRNA acylation tool facilitating genetic code reprogramming.
  • 2013From: Springer Protocols
    edited by Yinong Yang.
    Rice artificial hybridization for genetic analysis / Xueyan Sha -- Fluorescence in situ hybridization techniques for cytogenetic and genomic analyses / Jason G. Walling, Wenli Zhang, and Jiming Jiang -- Generation of rice mutants by chemical mutagenesis / Thomas H. Tai -- TILLING and ecotilling for rice / Jennifer L. Cooper [and others] -- Functional characterization of rice genes using a gene-indexed T-DNA insertional mutant population / Ki-Hong Jung and Gynheung An -- QTL analysis and map-based cloning of salt tolerance gene in rice / Ji-Ping Gao and Hong-Xuan Lin -- Site-specific gene integration in rice / Vibha Srivastava -- Transcriptome profiling analysis using rice oligonucleotide microarrays / Ki-Hong Jung and Pamela C. Ronald -- Cloning of small RNAs for the discovery of novel microRNAs in plants / Guru Jagadeeswaran and Ramanjulu Sunkar -- Global identification of small RNA targets in plants by sequencing sliced ends of messenger RNAs / Yong-Fang Li and Ramanjulu Sunkar -- Artificial microRNAs for specific gene silencing in rice / Norman Warthmann [and others] -- Rice proteomic analysis : sample preparation for protein identification / Ganesh Kumar Agrawal [and others] -- Quantification of jasmonic and salicylic acids in rice seedling leaves / Kyoungwon Cho [and others] -- Analysis of insect-induced volatiles from rice / Nan Zhao [and others] -- Phenotypic and physiological evaluation for drought and salinity stress responses in rice / Utlwang Batlang [and others] -- Phenotypic, physiological, and molecular evaluation of rice chilling stress response at the vegetative stage / Benildo G. de los Reyes [and others] -- Analysis of rice root hair morphology using cryo-scanning electron microscopy / Zhiming Yu -- Inoculation and virulence assay for bacterial blight and bacterial leaf streak of rice / Bing Yang and Adam Bogdanove -- Inoculation and scoring methods for rice sheath blight disease / Yulin Jia [and others] -- Molecular approaches to improve rice abiotic stress tolerance / Junya Mizoi and Kazuko Yamaguchi-Shinozaki -- Molecular strategies to improve rice disease resistance / Emily E. Helliwell and Yinong Yang -- Molecular strategies to engineer transgenic rice seed compartments for large-scale production of plant-made pharmaceuticals / Trevor Greenham and Illimar Altosaar.
  • 2016From: ClinicalKey
    [edited by] Todd E. Rasmussen, MD, FACS, Colonel, USAF MC, Director, U.S. Combat Casualty Care Research Program, Fort Dietrick, Maryland, Harris B. Shumacker, Jr. Professor of Surgery, The Norman M. Rich Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, Maryland, Attending Vascular & Trauma Surgeon, Veterans Administration Medical Center & University of Maryland Shock Trauma Center, Baltimore, Maryland, Nigel R.M. Tai, QHS, MS, FRCS(Gen), Colonel, L/RAMC, Clinical Director, Trauma Services, Royal London Hospital, Barts Health NHS Trust, London, United Kingdom, Senior Lecturer, Academic Department of Military Surgery and Trauma, Royal Centre for Defense Medicine, Birmingham, United Kingdom, Consultant Surgeon, 16 Medical Regiment, Colchester, Essex, United Kingdom.
    Published in association with the Society for Vascular Surgery (SVS), the newly updated edition of Rich's Vascular Trauma draws on civilian and military authorities from around the world to offer comprehensive and up-to-date coverage of the management of vascular injury. Anatomic patterns of vascular trauma including extremity, torso and cervical injury are reviewed in detail, including the latest techniques to manage non-compressible torso hemorrhage. You'll have access to all of the current innovations across the broad spectrum of vascular trauma, presented in a well-organized fashion that allows you to quickly hone-in on the most important issues in patient care and management.
  • 2012From: Springer
    Nicholas J. Mantis, editor.
    Interaction of Ricin and Shiga Toxins with Ribosomes -- How Ricin and Shiga Toxin Reach the Cytosol of Target Cells: Retrotranslocation from the Endoplasmic Reticulum -- Ricin and Shiga Toxins: Effects on Host Cell Signal Transduction -- Pathogenesis of Shiga-Toxin Producing Escherichia coli -- Shiga Toxin Pathogenesis: Kidney Complications and Renal Failure -- The Induction of Apoptosis by Shiga Toxins and Ricin -- Small-Molecule Inhibitors of Ricin and Shiga Toxins -- Immunity to Ricin: Fundamental Insights into Toxin-Antibody Interactions -- Animal Models of Ricin Toxicosis -- Ricin Vaccine Development.
  • 2014From: Springer
    Sean P. Gaine, Robert Naeije, Andrew John Peacock, editors.
    "This comprehensive book describes the structure, function and imaging of the normal right heart both at rest and under the stresses of high altitude and exercise. It continues with a detailed examination of the pathophysiology and pathobiology of right heart dysfunction, both in experimental models and human disease, including congenital heart disease. Right heart dysfunction caused by pulmonary hypertension is discussed in particular detail. The Right Heart brings together a distinguished group of contributors to discuss the advances in the topic that have occurred over the last few years and provides a concise and up-to-date resource for clinicians by compiling this comprehensive state-of-the-art text on the subject, the Editors have created an indispensable reference for future work by those interested in the cardiopulmonary circuit. This book will appeal to respiratory physicians, cardiologists and intensivists, all of whom will share the Editors' belief that the right heart is fundamental to integrated cardiopulmonary performance."
  • 2008From: Springer
    Ronald Eisenberg and Alexander Margulis.
  • Annie E. Casey Foundation.
  • 2016From: Karger
    volume editors, E. Beghi, G. Logroscino.
    The basic structure of a randomized clinical trial / Beghi, E -- Peculiarities of neurological disorders and study designs / Beghi, E., Pupillo, E. Giussani, G. -- Current issues in randomized clinical trials of neurodegenerative disorders at enrollment and reporting : diagnosis, recruitment, representativeness of patients, ethnicity, and quality of reporting / Logroscino, G., Capozzo, R., Tortelli, R., Marin, B. -- How to distinguish between statistically significant results and clinically relevant results / Bennett, D.A. -- Modeling and prediction in neurological disorders : the biostatistical perspective / Copetti, M. Fontana, A., Pellegrini, F. -- Composite scores and other outcome measures in stroke trial / Pistoia, F., Sacco, S., Ornello, R., Degan, D., Tiseo, C., Carolei, A. -- Age, comorbidity, frailty in observational and analytic studies of neurological diseases / Novy, J., Sander, J.W. -- Disease course, outcome measures, and prognostic predictors in epilepsy : opportunities for improving outcome of drug trials / Schmidt, D. -- Assessing functional decline in neurological diseases clinical trials : duration of follow-up : the case of multiple sclerosis / Martinelli Boneschi, F., Comi, G. -- Biomarkers in randomized clinical trials : magnetic resonance imaging / Whitwell, J.L. -- Biomarkers in randomized clinical trials : positron emission tomography and nuclear medicine techniques / Singhal, T., Stern, E -- Cerebrospinal fluid biomarkers for target engagement and efficacy in clinical trials for Alzheimer's and Parkinson's diseases / Parnetti, L., Eusebi, P., Lleó, A -- Pharmacogenetics in neurodegenerative diseases: implications for clinical trials / Tortelli, R., Seripa, D., Panza, F., Solfrizzi, V., Logroscino, G. -- Randomized trials in developing countries : different priorities and study design? / Marin, B., Agbota, G.C., Preux, P.-M., Boumédiène, F. -- The right therapy for neurological disorders : from randomized trials to clinical practice : patients versus investigator expectations and needs / Bruijn, L.I., Kolb, S. -- General overview, conclusions, and future directions / Beghi, E., Logroscino, G.
  • 2012From: Springer
    Massimo Chessa, Alessandro Giamberti, editors ; foreword by Gary D. Webb.
    Introduction / Massimo Chessa and Alessandro Giamberti -- Anatomy of Tetralogy of Fallot / Carla Frescura and Gaetano Thiene -- Genetics / M. Cristina Digilio, Bruno Dallapiccola and Bruno Marino -- Stem Cells and the Right Ventricle / Luigi Anastasia and Marco Piccoli -- Pathophysiology in Tetralogy of Fallot / Gabriele Egidy Assenza and Michael J. Landzberg -- Tetralogy of Fallot: Late Outcome / Jochen Weil -- Tetralogy of Fallot: the Failing Right Ventricle / Folkert J. Meijboom and Barbara Mulder -- Imaging Evaluation / Claudio Bussadori -- Timing for RVOT Management / Harald Kaemmerer, Andreas Eicken and John Hess -- Percutaneous Pulmonary Valve / Mark S. Turner, Mario Carminati and Philipp Bonhoeffer -- Other Transcatheter Procedures / Massimo Chessa and Gianfranco Butera -- Surgical Pulmonary Valve Implantation / Alessandro Giamberti, Giuseppe Pomè and Alessandro Frigiola -- Other Surgical Procedures / Alessandro Giamberti -- Late Arrhythmias: Current Approaches / Sara Foresti, Maria Cristina Tavera, Pierpaolo Lupo and Riccardo Cappato -- Perioperative Right Ventricular Management / Marco Ranucci.
  • 2015From: Springer
    Norbert F. Voelkel, Dietmar Schranz, editors.
    The Right Ventricle in Health and Disease provides a comprehensive and up-to-date database and collection of the available information which describes the structure and function of the normal right ventricle. The right ventricular performance and function reserve has now finally moved to the center of the stage as clinicians recognize that the drugs presently used to treat patients with severe pulmonary hypertension do not necessarily improve the performance of the right ventricle and because the survival depends on the right ventricular function that treatment strategies need to be developed to primarily protect the right ventricle from failing. In-depth chapters discuss right heart function and failure in patients with congenital heart diseases, review modern imaging techniques used to describe right ventricular form and function in patients with right heart failure (including cardiac MRI and PET scanning), describe ventricular interdependence: the left ventricle in pulmonary hypertension and discuss the concept of the sick lung circulation and its contribution to right heart failure. Treatment strategies of chronic right heart failure including drugs and mechanical devices are also discussed.
  • 2015From: Cambridge
    Joseph J. Fins.
    Decisions -- The injury -- Coming to terms with brain injury -- The origins of the vegetative state -- A shift since Quinlan -- Maggie's wishes -- Something happened in Arkansas -- From PVS to MCS -- Leaving the hospital -- Heather's story -- Neuroimaging and neuroscience in the public mind -- Contractures and contradictions : medical necessity and the injured brain -- Minds, monuments, and moments -- Heads and hearts, toil and tears -- What do families want? -- Deep brain stimulation in MCS -- Mending our brains, minding our ethics -- It's still freedom -- Maggie's in town -- When consciousness becomes prosthetic -- The rights of mind -- A call for advocacy.
  • Richard Harris.
    PrintStatus: Not Checked OutLane Catalog Record
    American taxpayers spend $30 billion annually funding biomedical research. By some estimates, half of the results from these studies can't be replicated elsewhere -- the science is simply wrong. Often, research institutes and academia emphasize publishing results over getting the right answers, incentivizing poor experimental design, improper methods, and sloppy statistics. Bad science doesn't just hold back medical progress, it can sign the equivalent of a death sentence. How are those with breast cancer helped when the cell on which 900 papers are based turns out not to be a breast cancer cell at all? How effective could a new treatment for ALS be when it failed to cure even the mice it was initially tested on? Science journalist Richard F. Harris reveals these urgent issues with anecdotes, personal stories, and interviews with the nation's top biomedical researchers.
  • Johanna Roberta Schaub.
    Primary cilia have critical sensory and signaling roles in development and disease. These functions rely on the proper localization of structural and signaling proteins to the cilium. Ciliary trafficking is the movement of proteins to, within, and from the cilium, processes that are regulated by several classes of proteins, for example: endo- and exocytic machinery, diffusion barriers, and motor proteins. To understand how ciliary trafficking is regulated we used gene expression data from multi-ciliated cells to identify two proteins involved in ciliary trafficking: the Rilp-like proteins Rilpl1 and Rilpl2. We took three approaches to characterizing these proteins: 1) examining localization of Rilpl1 and Rilpl2 in mammalian cells, 2) determining the phenotype of cells depleted of Rilpl1 and/or Rilpl2, and 3) identifying interaction partners. We found that Rilpl2 is up-regulated during ciliogenesis and localizes to the centrosome and primary cilium. Rilpl1 also localizes to the primary cilium and is a distal mother centriole protein with cell-cycle dependent localization. Additionally, Rilp-like proteins form dynamic tubules from the base of the cilium, which are microtubule dependent and induced by disruption of the actin network. Depletion of Rilpl1 and/or Rilpl2 from ciliated cells leads to an accumulation of signaling proteins in the ciliary membrane and disrupts epithelial cell organization in three-dimensional culture. We also determined that both Rilpl1 and Rilpl2 interact with Rab36. Taken together, our data suggest that Rilp-like proteins control ciliary protein content through the regulation of ciliary protein removal.
  • 2006From: ScienceDirect
    edited by Thomas Barrett, Paul-Pierre Pastoret, and William P. Taylor.
  • 2006From: Springer
    edited by Alison Jolly ... [et al.].
    The distribution and biogeography of the ringtailed lemur (Lemur catta) in Madagascar / Steven M. Goodman, Soava V. Rakotoarisoa and Lucienne Wilmé -- A preliminary estimate of Lemur catta population density using satellite imagery / Robert W. Sussman ... [et al.] -- Berenty Reserve : a research site in southern Madagascar / Alison Jolly ... [et al.] -- Beza Mahafaly Special Reserve : a research site in southwestern Madagascar / Robert W. Sussman and Joelisoa Ratsirarson -- Plant species fed on by Lemur catta in gallery forests of the southern domain of Madagascar / Bruno Simmen ... [et al.] -- Tamarind recruitment and long-term stability in the gallery forest at Berenty, Madagascar / Kathryn Blumenfeld-Jones ... [et al.] -- Home ranges of ringtailed lemur troops and the density of large trees at Berenty Reserve, Madagascar / Naoki Koyama ... [et al.] -- The influence of tamarind tree quality and quantity on Lemur catta behavior / Anne S. Mertl-Millhollen ... [et al.] -- Feeding competition between introduced Eulemur fulvus and native Lemur catta during the birth season at Berenty Reserve, southern Madagascar / Susan Pinkus, James N.M. Smith and Alison Jolly -- Tradition and novelty : Lemur catta feeding strategy on introduced tree species at Berenty Reserve / Takayo Soma -- Diet quality and taste perception of plant secondary metabolites by Lemur catta / B. Simmen ... [et al.] -- Territory as bet-hedging : Lemur catta in a rich forest and an erratic climate / Alison Jolly ... [et al.] -- Resource defense in Lemur catta : the importance of group size / R. Ethan Pride ... [et al.] -- Social changes in a wild population of ringtailed lemurs (Lemur catta) at Berenty, Madagascar / Shinichiro Ichino and Naoki Koyama -- Obsession with agonistic power / Michael. E. Pereira -- Male and female ringtailed lemurs' energetic strategy does not explain female dominance / Hantanirina Rasamimanana ... [et al.] -- Male sociality and integration during the dispersal process in Lemur catta : a case study / Lisa Gould -- Patterns of health, disease, and behavior among wild ringtailed lemurs, Lemur catta: effects of habitat and sex / Michelle L. Sauther ... [et al.] -- Bald lemur syndrome and the miracle tree : alopecia associated with Leucaena leucocephala at Berenty Reserve, Madagascar / Graham C. Crawford ... [et al.] -- Temporal change in tooth size among ringtailed lemurs (Lemur catta) at the Beza Mahafaly Special Reserve, Madagascar : effects of an environmental fluctuation / Frank P. Cuozzo and Michelle L. Sauther.
  • Jonathan Hughes.
    Pt. 1. Alchemy and the occult in English cultural and political life. Introduction : alchemy and the occult in England ; The origins of English alchemy and sources for the study of alchemy in fourteenth-century England -- Pt. 2. The Astrology of the earth : the intellectual impact of alchemy in fourteenth-century England. Alchemy and science and philosophy ; Alchemy and the Church ; The Cultural impact of alchemy -- Pt. 3. The Political significance of alchemy. The accession of Edward III ; Alchemical themes in the kingship of Edward III ; Richard II the magus and boy king ; Richard II and the occult ; Conclusion : the Lancastrian reaction.
  • 2013From: Springer
    Lawrence D. Longo ; foreword by John R.G. Challis.
    During the mid- to late-twentieth century, study of the physiology of the developing fetus and newborn infant evolved rapidly to become a major discipline in the biomedical sciences. Initially of interest from a standpoint of function of the placenta and oxygenation of the fetus, the field advanced to explore both normal functional mechanisms as well as pathophysiologic aspects of their regulation. Examples include studying the role and regulation of circulatory vascular anatomic shunts in oxygenation, cardiac function, certain aspects of asphyxia in the fetus and newborn infant, the role of fetal breathing movements, cyclic electroencephalographic activity, and analysis of electronic monitoring of fetal heart rate variability and its significance. Included in this book are reminisces of several dozen individuals who played a vital role in these developments. Overall, this survey considers a number of aspects of the development of the science of fetal and neonatal physiology, and its role in the greatly improved care of pregnant women and their newborn infants.
  • Lisa I. Iezzoni, editor.
    Reasons for risk adjustment / Lisa I. Iezzoni -- Getting started and defining terms / Lisa I. Iezzoni -- Range of risk factors / Lisa I. Iezzoni -- Windows of observation / Amy K. Rosen and Ann M. Borzecki -- Coded data from administrative sources / Lisa I. Iezzoni -- Clinical data from medical records and health information technologies / Lisa I. Iezzoni -- Data from patient surveys / Lisa I. Iezzoni and Karen Donelan -- Conceptual and practical issues in developing risk adjustment methods / Lisa I. Iezzoni -- Validity and reliability of risk adjustment methods / Lisa I. Iezzoni -- Empirically evaluating risk adjustment models / Michael Shwartz and Arlene S. Ash -- Estimating the effect of an intervention from observational data / Michael Shwartz and Arlene S. Ash -- Comparing outcomes across providers / Arlene S. Ash ... [et al.] -- Risk adjustment in pediatric populations / Karen Kuhlthau, Jeanne Van Cleave, and Timothy G. Ferris -- Risk adjustment for mental health care / Anthony P. Weiss and Mark A. Blais -- Risk adjustment and persons with disabilities / Lisa I. Iezzoni -- Risk adjustment for long-term care / Dan Berlowitz and Orna Intrator -- The role of risk adjustment in managing health care organizations / Jennifer Daley -- Final observations / Lisa I. Iezzoni.
  • 2007From: Springer
    edited by Manfred S. Green, Jonathan Zenilman, Dani Cohen, Itay Wiser, and Ran D. Balicer.
  • PrintStatus: Not Checked OutLane Catalog Record
  • PrintStatus: Not Checked OutLane Catalog Record
  • "Vibrio parahaemolyticus are common causes of diarrhoeal disease worldwide. These marine micro-organisms, native in estuarine waters globally, concentrate in the gut of filter-feeding molluscan shellfish, such as oysters, clams and mussels. Raw and undercooked seafood, including finfish, represent the principal vehicle of transmission to humans. This volume considers the applicability of an assessment of the public health impact of raw oyster consumption, developed in one country, to assess the public health risk associated with the consumption of raw oysters harvested in other countries where different growing and harvesting scenarios might exist. The approach is also applied to bloody clams and finfish to establish if such a risk assessment can also be adapted to other types of shellfish and finfish. This work is therefore divided in three parts focusing on (i) risk assessment of Vibrio parahaemolyticus in raw oysters, (ii) risk assessment of Vibrio parahaemolyticus in bloody clam and (iii) risk assessment of Vibrio parahaemolyticus in finfish. As well as providing insights on the risks associated with consumption of these commodities, the work also addresses how to make maximum use of existing and/or limited resources"--P. [4] of cover.
  • World Health Organization, Food and Agriculture Organization of the United Nations.
    PrintStatus: Not Checked OutLane Catalog Record
  • 2016From: Oxford Medicine Online
    edited by Sudha Seshadri and Stéphanie Debette.
    This text, by leading stroke researchers from Europe and North America, describes environmental and genetic risk factors for cerebrovascular disease. The section on genetics is exceptionally detailed and contemporaneous.
  • 2011From: ClinicalKey
    Phil Haeck, Mark Gorney.
    "Risk, Liability and Malpractice: What Every Plastic Surgeon Need To Know" is your indispensable guide to maintaining a reputable, safe, successful plastic surgery practice. Authors Phil Haeck, MD and Mark Gorney, MD, two of the best-known and most trusted authorities on this subject, discuss every issue you need to consider, from which procedures carry the highest risk through claims arising from Medi-Spas and unique aspects of providing aesthetic surgery for male patients. Written in a concise and easy-to-read style, this is the book you need to make wise clinical and practice decisions, manage your patients' expectations, avoid complications, and deliver satisfying results. Effectively navigate your toughest legal issues! Must-read chapters include "The Most Risky Procedures in Plastic Surgery," "Claims Arising From Medi-Spas," and "The Unique Aspects of The Male Patient and Aesthetic Surgery." Get advice you can trust! Phil Haeck, MD has been writing Plastic Surgery News's "On Legal Grounds" column for more than five years and is the 2011/2012 president of ASPS. Mark Gorney, MD, a former ASPS president, is a founding member of The Doctors Company, now the country's largest malpractice insurance carrier, and has taught the basics in risk management to thousands of his colleagues. Read it quickly and put it to work in your practice! A concise, practical approach with minimal legal jargon presents the guidance you need in a way that is easy to understand and apply.
  • 2016From: Springer
    Walter Merkle, editor.
    Why do we wrong, although we want to do right?- Current view of German Authorities -- Human behavior in process management -- Successful strategies to detect and avoid failures -- Risk management in aviation -- Risk management in medicine ? principles -- Risk management in the US medicine -- Systemic coaching of staff as risk management tool -- CIRS (Critical Incident Reporting System) -- OTAS (Observational Teamwork Assessment for Surgery) -- FMEA (Failure Mode and Effects Analysis) -- TTO (Team Time Out) -- Peer-Review.- CRM (Crew Ressource Management).- Law aspects-Germany -- Law aspects -- Europe -- Law Aspects -- USA -- Insurances problems -- Implementation of risk management in hospitals -- Economic aspects of risk management -- ISO in medical risk management.
  • 2008From: Springer
    Marvin F. Kraushar, editor.
    Part 1 : General considerations -- Basic medical malpractice terminology -- The defense attorney -- The plaintiff's attorney -- The trial judge -- The medical expert -- Sequence of events in a medical malpractice lawsuit -- Informed consent -- The five most effective risk prevention strategies -- Revelation of adverse events and the conundrum of an apology -- Part 2 : Risk management in the ophthalmic subspecialties and related fields -- Anesthesia -- Cataract surgery -- Cornea : external disease and contact lenses -- Drugs -- Emergencies -- Glaucoma -- Neuroophthalmology -- Oncology -- Ophthalmic plastic surgery -- Pediatric ophthalmology -- Refractive corneal surgery -- Retina and vitreous -- Uveitis -- Part 3 : Personal and business considerations -- Physician and defendant : living and coping with a double life -- Medical malpractice insurance : selection of companies and policies.
  • 2009From: Springer
    rédacteurs: M. Fieschi ... [et al.].
  • edited by Glenn Frankel ; designed by Kelly Dubeau.
    PrintStatus: Not Checked OutLane Catalog Record
    The Heart of the matter : for one family with a five-year-old with chronic disease, normal life is the greatest gift / by Kathryn Roethel -- Bus to nowhere : for Silicon Valley's homeless population, Hotel 22 is an endless ride / by Miranda Simon -- The Good death : "No One Dies Alone" is a volunteer program and a promise / by Ryan Mac -- The Clean room : why did so many IBM employees come down with cancer after working in Silicon Valley? / by Lily Bixler -- Shelter from the storm : in a time of budgetary crisis, abused women still need help / by Kristen Barta -- Punched in the gut : can a family ever recover from an eating disorder? / by Erin Beresini.
  • 2008From: Springer Protocols
    edited by Henrik Nielsen.
    The transcriptional landscape -- Working with RNA -- Synthesis of RNA by in vitro transcription -- Efficient poly(A)+ RNA selection using LNA oligo(T) capture -- Genome browsers -- Web-based tools for studying RNA structure and function -- Northern blotting analysis -- Rapid amplification of cDNA ends (RACE) -- Fractionation of mRNA based on the length of the poly(A) tail -- Analysis of mutations that influence pre-mRNA splicing -- S1 nuclease analysis of alternatively spliced mRNA -- Promises and challenges in developing RNAi as a research tool and therapy -- Inhibition of gene function in mammalian cells using short-hairpin RNA (shRNA) -- Validation of RNAi by real time PCR -- Profiling RNA polymerase II using the fast chromatin immunoprecipitation method -- The post-transcriptional operon -- RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray (chip) profiling -- Isolation of RNP granules -- Electrophoretic mobility shift assay for characterizing RNA-protein interaction -- Polysome analysis and RNA purification from sucrose gradients -- Prediction of targets for microRNAs -- Outsourcing of experimental work.
  • 2012From: Springer Protocols
    edited by Hailing Jin, Walter Gassmann.
    SuperSAGE : powerful serial analysis of gene expression / Hideo Matsumura [and others] -- Northern blot analysis for expression profiling of mRNAs and small RNAs / Ankur R. Bhardwaj [and others] -- Construction of RNA-seq libraries from large and microscopic tissues for the illumina sequencing platform / Hagop S. Atamian and Isgouhi Kaloshian -- Strand-specific RNA-seq applied to malaria samples / Nadia Ponts, Duk-Won D. Chung, and Karine G. Le Roch -- RNA in situ hybridization in Arabidopsis / Miin-Feng Wu and Doris Wagner -- Laser microdissection of cells and isolation of high-quality RNA after cryosectioning / Marta Barcala, Carmen Fenoll, and Carolina Escobar -- Detection and quantification of alternative splicing variants using RNA-seq / Douglas W. Bryant Jr, Henry D. Priest, and Todd C. Mockler -- Separating and analyzing sulfur-containing RNAs with Organomercury Gels / Elisa Biondi and Donald H. Burke -- RNAse mapping and quantitation of RNA isoforms / Lakshminarayan K. Venkatesh, Olufemi Fasina, and David J. Pintel -- Detection and quantification of viral and satellite RNAs in plant hosts / Sun-Jung Kwon, Jang-Kyun Seo, and A.L.N. Rao -- In situ detection of mature miRNAs in plants using LNA-modified DNA probes / Xiaozhen Yao, Hai Huang, and Lin Xu -- Small RNA isolation and library construction for expression profiling of small RNAs from neurospora and fusarium using illumina high-throughput deep sequencing / Gyungsoon Park and Katherine A. Borkovich -- Isolation and profiling of protein-associated small RNAs / Hongwei Zhao [and others] -- New virus discovery by deep sequencing of small RNAs / Kashmir Singh, Ravneet Kaur, and Wenping Qiu -- Global assembly of expressed sequence tags / Foo Cheung -- Computational analysis of RNA-seq / Scott A. Givan, Christopher A. Bottoms, and William G. Spollen -- Identification of microRNAs and natural antisense transcript-originated endogenous siRNAs from small-RNA deep sequencing data / Weixiong Zhang [and others].
  • 2017From: Springer
    Long-Cheng Li, editor.
    This book offers an essential guide to RNA activation (RNAa), an emerging and fascinating new field. RNAa is a small RNA-guided and Argonaute-dependent gene regulation phenomenon in which promoter-targeted short double-stranded RNAs (dsRNAs) induce target gene expression at the transcriptional level. It occurs primarily in the nucleus and can be mediated by artificially designed short duplex RNAs that target regulatory sequences (e.g., promoters, genes' 3' termini and enhancers) and naturally occurring small RNAs (e.g., miRNAs and C. elegans 22G-RNAs). With contributions from internationally respected RNA experts, this book provides comprehensive coverage of different RNAa mechanisms and a timely update on recent advances in RNAa research, with a focus on developing RNAa-based therapeutics. Special chapters are also devoted to the topics of gene activation induced by antisense oligonucleotides and the CRISPR system. As the first book to cover RNAa, it will be of interest to a wide audience, from scientists in academia and the pharmaceutical industry to clinicians who wish to further explore the biology of RNAa and related phenomena, so as to harness their full potential for use in biotechnology and drug development.
  • 2013From: Springer
    edited by Jane Wu.
    Coupling Between Transcription and Alternative Splicing / Ignacio E. Schor, Luciana I. Gómez Acuña, Alberto R. Kornblihtt -- Detection of Alternatively Spliced or Processed RNAs in Cancer Using Oligonucleotide Microarray / Marieta Gencheva ... [et al.] -- Cancer-Associated Perturbations in Alternative Pre-messenger RNA Splicing / Lulzim Shkreta ... [et al.] -- Alternative Splicing of Tumor Suppressors and Oncogenes / Claudia Ghigna, Silvano Riva, Giuseppe Biamonti -- MicroRNAs in Cancer / Jianzhong Jeff Xi -- The Perinucleolar Compartment: RNA Metabolism and Cancer / John T. Norton, Sui Huang -- Regulation of ARE-mRNA Stability by Cellular Signaling: Implications for Human Cancer / Christian Kroun Damgaard, Jens Lykke-Andersen -- Alternative Pre-mRNA Splicing, Cell Death, and Cancer / Kong Ruirui ... [et al.] -- Oligonucleotide Therapeutics in Cancer / Jing Wan, John A. Bauman, Maria Anna Graziewicz, Peter Sazani -- Clinical Perspective on Chemo-Resistance and the Role of RNA Processing / Nancy L. Krett, Shuo Ma, Steven T. Rosen.
    Also available: Print – 2013
  • 2011From: Springer Protocols
    edited by Ruslan Aphasizhev.
    Three-dimensional reconstruction of trypanosoma brucei editosomes using single-particle electron microscopy / H.U. Goringer ... [et al.] -- Icoda: RNAi-based inducible knock-in system in trypanosoma brucei / G.E. Ringpis, R.H. Lathrop and R. Aphasizhev -- Perturbing a-to-i RNA editing using genetics and homologous recombination / C.J. Staber ... [et al.] -- Laser microdissection and pressure catapulting of single human motor neurons for RNA editing analysis / H. Sun ... [et al.] -- Biochemical identification of A-to-I RNA editing sites by the inosine chemical erasing (ice) method / M. Sakurai and T. Suzuki -- Identifying mRNA editing deaminase targets by RNA-seq / B.R. Rosenberg, S. Dewell and F.N. Papavasiliou -- Mouse and other rodent models of C to U RNA editing / V. Blanc and N.O. Davidson -- In vivo analysis of RNA editing in plastids / S. Ruf and R. Bock -- Identifying specific trans-factors of RNA editing in plant mitochondria by multiplex single base extension typing / M. Takenaka -- Complementation of mutants in plant mitochondrial RNA editing by protoplast transfection / M. Takenaka and A. Zehrmann -- A high-throughput assay for DNA deaminases / M. Wang, C. Rada and M.S. Neuberger -- Biochemical fractionation and purification of high-molecular-mass apobec3g complexes / Y.L. Chiu -- Analysis of tRNA editing in native and synthetic substrates / J.L. Spears, K.W. Gaston and J.D. Alfonzo -- Post-transcriptional modification of RNAs by artificial box h/aca and box c/d rnps / C. Huang, J. Karijolich and Y.T. Yu -- Functional analysis of noncoding RNAs in trypanosomes: RNA walk, a novel approach to study RNA-RNA interactions between small RNA and its target / C. Wachtel and S. Michaeli -- A post-labeling approach for the characterization and quantification of RNA modifications based on site-directed cleavage by dnazymes / M. Meusburger, M. Hengesbach and M. Helm.
  • 2015From: Springer Protocols
    edited by Ernesto Picardi.
    Free energy minimization to predict RNA secondary structures and computational RNA design / Alexander Churkin, Lina Weinbrand, and Danny Barash -- RNA secondary structure prediction from multi-aligned sequences / Michiaki Hamada -- Simple protocol for the inference of RNA global pairwise alignments / Eugenio Mattei, Manuela Helmer-Citterich, and Fabrizio Ferrè -- De novo secondary structure motif discovery using RNA profile / Federico Zambelli and Giulio Pavesi -- Drawing and editing the secondary structure(s) of RNA / Yann Ponty and Fabrice Leclerc -- Modeling and predicting RNA three-dimensional structures / Jérôme Waldispühl and Vladimir Reinharz -- Fast prediction of RNA-RNA interaction using heuristic algorithm / Soheila Montaseri -- Quality control of RNA-Seq experiments / Xing Li ... [and 3 others] -- Accurate mapping of RNA-Seq data / Kin Fai Au -- Quantifying entire transcriptomes by aligned RNA-Seq data / Raffaele A. Calogero and Francesca Zolezzi -- Transcriptome assembly and alternative splicing analysis / Paola Bonizzoni ... [and 5 others] -- Detection of post-transcriptional RNA editing events / Ernesto Picardi ... [and 3 others] -- Prediction of miRNA targets / Anastasis Oulas ... [and 6 others] -- Using deep sequencing data for identification of editing sites in mature miRNAs / Shahar Alon and Eli Eisenberg -- NGS-trex : an automatic analysis workflow for RNA-seq data / Ilenia Boria ... [and 3 others] -- e-DNA meta-barcoding : from NGS raw data to taxonomic profiling / Fosso Bruno, Marzano Marinella, and Monica Santamaria -- Deciphering metatranscriptomic data / Evguenia Kopylova ... [and 6 others] -- RIP-seq data analysis to determine RNA-protein associations / Federico Zambelli and Giulio Pavesi -- ViennaRNA eeb services / Andreas R. Gruber, Stephan H. Bernhart, and Ronny Lorenz -- Exploring the RNA editing potential of RNA-Seq data by ExpEdit / Mattia D'Antonio ... [and 4 others] -- Guideline for the annotation of UTR regulatory elements in the UTRsite collection / Matteo Giulietti ... [and 3 others] -- Rfam : annotating families of non-coding RNA sequences / Jennifer Daub ... [and 3 others] -- ASPicDB : a database web tool for alternative splicing analysis / Mattia D'Antonio ... [and 5 others] -- Analysis of alternative splicing events in custom gene datasets by AStalavista / Sylvain Foissac and Michael Sammeth -- Computational design of artificial RNA molecules for gene regulation / Alessandro Laganà ... [and 6 others].
  • 2012From: Springer
    Neocles Leontis, Eric Westhof, editors.
    Introduction / Michael Levitt -- Modeling RNA Molecules / Neocles Leontis and Eric Westhof -- Methods for Predicting RNA Secondary Structure / Kornelia Aigner, Fabian Dre€en and Gerhard Steger -- Why Can't We Predict RNA Structure At Atomic Resolution? / Parin Sripakdeevong, Kyle Beauchamp and Rhiju Das -- Template-Based and Template-Free Modeling of RNA 3D Structure: Inspirations from Protein Structure Modeling / Kristian Rother, Magdalena Rother, Michał Boniecki, Tomasz Puton and Konrad Tomala, et al. -- The RNA Folding Problems: Different Levels of sRNA Structure Prediction / Fredrick Sijenyi, Pirro Saro, Zheng Ouyang, Kelly Damm-Ganamet and Marcus Wood, et al. -- Computational Prediction and Modeling Aid in the Discovery of a Conformational Switch Controlling Replication and Translation in a Plus-Strand RNA Virus / Wojciech K. Kasprzak and Bruce A. Shapiro -- Methods for Building and Refining 3D Models of RNA / Samuel C. Flores, Magdalena Jonikas, Christopher Bruns, Joy P. Ku and Jeanette Schmidt, et al. -- Multiscale Modeling of RNA Structure and Dynamics / Feng Ding and Nikolay V. Dokholyan -- Statistical Mechanical Modeling of RNA Folding: From Free Energy Landscape to Tertiary Structural Prediction / Song Cao and Shi-Jie Chen -- Simulating Dynamics in RNA-Protein Complexes / John Eargle and Zaida Luthey-Schulten -- Quantum Chemical Studies of Recurrent Interactions in RNA 3D Motifs / Jiří Šponer, Judit E. Šponer and Neocles B. Leontis -- Nonredundant 3D Structure Datasets for RNA Knowledge Extraction and Benchmarking / Neocles B. Leontis and Craig L. Zirbel -- Ions in Molecular Dynamics Simulations of RNA Systems / Pascal Auffinger -- Modeling RNA Folding Pathways and Intermediates Using Time-Resolved Hydroxyl Radical Footprinting Data / Joshua S. Martin, Paul Mitiguy and Alain Laederach -- A Top-Down Approach to Determining Global RNA Structures in Solution Using NMR and Small-Angle X-ray Scattering Measurements / Yun-Xing Wang, Jinbu Wang and Xiaobing Zuo -- RNA Structure Determination by Structural Probing and Mass Spectrometry: MS3D / A. E. Hawkins and D. Fabris.
  • 2011From: Springer Protocols
    edited by Jeffrey E. Gerst.
    Single molecule imaging of RNA in situ / Mona Batish, Arjun Raj, and Sanjay Tyagi -- FISH and immunofluorescence staining in Chlamydomonas / James Uniacke, Daniel Colón-Ramos, and William Zerges -- High resolution fluorescent in situ hybridization in Drosophila / Eric Lécuyer -- Localization and anchorage of maternal mRNAs to cortical structures of ascidian eggs and embryos using high resolution in situ hybridization / Alexandre Paix, Janet Chenevert, and Christian Sardet -- Visualization of mRNA localization in Xenopus oocytes / James A. Gagnon and Kimberly L. Mowry -- Visualization of mRNA expression in the zebrafish embryo / Yossy Machluf and Gil Levkowitz -- High-resolution fluorescence in situ hybridization to detect mRNAs in neuronal compartments in vitro and in vivo / Sharon A. Swanger, Gary J. Bassell, and Christina Gross -- Localization of mRNA in vertebrate axonal compartments by in situ hybridization / José Roberto Sotelo-Silveira ... [et al.] -- Tiny molecular beacons for in vivo mRNA detection / Diana P. Bratu, Irina E. Catrina, and Salvatore A.E. Marras -- Delivery of molecular beacons for live-cell imaging and analysis of RNA / Antony K. Chen ... [et al.] -- Genetically-encoded fluorescent probes for imaging endogenous mRNA in living cells / Takeaki Ozawa and Yoshio Umezawa -- Visualization of induced RNA in single bacterial cells / Azra Borogovac and Natalia E. Broude -- Visualizing mRNAs in fixed and living yeast cells / Franck Gallardo and Pascal Chartrand -- In vivo visualization of RNA using the U1A-based tagged RNA system / Sunglan Chung and Peter A. Takizawa -- Visualizing endogenous mRNAs in living yeast using m-TAG, a PCR-based RNA aptamer integration method, and fluorescence microscopy / Liora Haim-Vilmovsky and Jeffrey E. Gerst -- Imaging mRNAs in living mammalian cells / Sharon Yunger and Yaron Shav-Tal -- Using the mRNA-MS2/MS2CP-FP system to study mRNA transport during Drosophila oogenesis / Katsiarina Belaya and Daniel St Johnston -- Genome-wide analysis of RNA extracted from isolated mitochondria / Erez Eliyahu, Daniel Melamed and Yoav Arava -- Analyzing mRNA localization to the endoplasmic reticulum via cell fractionation / Sujatha Jagannathan, Christine Nwosu and Christopher V. Nicchitta -- Isolation of mRNAs encoding peroxisomal proteins from yeast using a combined cell fractionation and affinity purification procedure / Gadi Zipor, Cecile Brocard and Jeffrey E. Gerst -- Profiling axonal mRNA transport / Dianna E. Willis and Jeffery L. Twiss -- RNA purification from tumor cell protrusions using porous polycarbonate filters / Jay Shankar and Ivan R. Nabi -- RNA-binding protein immunopurification-microarray (RIP-chip) analysis to profile localized RNAs / Alessia Galgano and André P. Gerber -- RaPID : an aptamer-based mRNA affinity purification technique for the identification of RNA and protein factors present in ribonucleoprotein complexes / Boris Slobodin and Jeffrey E. Gerst -- RIP : an mRNA localization technique / Sabarinath Jayaseelan ... [et al.] -- The dual use of RNA aptamer sequences for affinity purification and localization studies of RNAs and RNA-protein complexes / Scott C. Walker ... [et al.] -- Identifying and searching for conserved RNA localisation signals / Russell S. Hamilton and Ilan Davis -- Computational prediction of RNA structural motifs involved in post-transcriptional regulatory processes / Michal Rabani, Michael Kertesz, and Eran Segal.
  • 2007From: ScienceDirect
    edited by Jonatha M. Gott.
    Also available: Print – 2007
  • 2008From: Springer
    H. Ulrich Göringer (ed.).
    Editing reactions from the perspective of RNA structure / Matthias Homann -- Editing of tRNA for structure and function / Juan D. Alfonzo -- RNA editing by adenosine deaminases that act on RNA (ADARs) / Michael F. Jantsch and Marie Öhman -- Insertion/deletion editing in Physarum polycephalum / Jonatha M. Gott and Amy C. Rhee -- RNA editing in plant mitochondria / Mizuki Takenaka ... [et al.] -- RNA editing in chloroplasts / Masahiro Sugiura -- Working together : the RNA editing machinery in Trypanosoma brucei / Jason Carnes and Kenneth Stuart -- RNA editing accessory factors : the example of mHel61p / H. Ulrich Göringer ... [et al.] -- The function of RNA editing in trypanosomes / Torsten Ochsenreiter and Stephen Hajduk -- Evolutionary aspects of RNA editing / Dave Speijer.
  • 2011From: Springer
    edited by Torben Heick Jensen.
    "The diversity of RNAs inside living cells is amazing. We have known of the more "classic" RNA species: mRNA, tRNA, rRNA, snRNA and snoRNA for some time now, but in a steady stream new types of molecules are being described as it is becoming clear that most of the genomic information of cells ends up in RNA. To deal with the enormous load of resulting RNA processing and degradation reactions, cells need adequate and efficient molecular machines. The RNA exosome is arising as a major facilitator to this effect. Structural and functional data gathered over the last decade have illustrated the biochemical importance of this multimeric complex and its many co-factors, revealing its enormous regulatory power. By gathering some of the most prominent researchers in the exosome field, it is the aim of the book to introduce this fascinating protein complex and to give a timely and rich account of its many functions"--Provided by publisher.
    Also available: Print – 2011
  • 2014From: Springer Protocols
    edited by Christina Waldsich.
    Predicting RNA Structure: Advances and Limitations -- Cis-Acting 5' Hammerhead Optimization for In Vitro Transcription of Highly Structured RNAs -- RNA Structural Analysis by Enzymatic Digestion -- A Mutate-and-Map Protocol for Inferring Base Pairs in Structured RNA -- Mapping RNA Structure In Vitro Using Nucleobase-Specific Probes -- Massively Parallel RNA Chemical Mapping with a Reduced Bias MAP-Seq Protocol -- Probing RNA Folding by Hydroxyl Radical Footprinting -- Monitoring Global Structural Changes and Specific Metal Binding Sites in RNA by In-Line Probing and Tb(III) Cleavage -- Chemical Probing of RNA in Living Cells -- A Chemogenetic Approach to Study the Structural Basis of Protein-Facilitated RNA Folding -- Applying UV Crosslinking to Study RNA-Protein Interactions in Multicomponent Ribonucleoprotein Complexes -- The Kinetics of Ribozyme Cleavage: A Tool to Analyze RNA Folding as a Function of Catalysis -- RNA Catalytic Activity as a Probe of Chaperone-Mediated RNA Folding -- Evaluation of RNA Chaperone Activity In Vivo and In Vitro Using Misfolded Group I Ribozymes -- RNA Conformational Changes Analyzed by Comparative Gel Electrophoresis -- Detecting RNA Tertiary Folding by Sedimentation Velocity Analytical Ultracentrifugation -- RNA Folding Dynamics Using Laser Assisted Single-Molecule Refolding -- RNA Refolding Studied by Light-Coupled NMR Spectroscopy -- Exploring RNA Oligomerization and Ligand Binding by Fluorescence Correlation Spectroscopy and Small Angle X-Ray Scattering.
  • 2012From: ScienceDirect
    edited by Eckhard Jankowsky.
    This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases. The contributions in this volume cover the broad scope of methods in the research on these enzymes. Several chapters describe quantitative biophysical and biochemical approaches to study molecular mechanisms and conformational changes of RNA helicases. Further chapters cover structural analysis, examination of co-factor effects on several representative examples, and the analysis of cellular functions of select enzymes. Two chapters outline approaches to the analysis of inhibitors that target RNA helicases. This volume of Methods in Enzymology aims to provide a reference for the diverse, powerful tools used to analyze RNA helicases The contributions in this volume cover the broad scope of methods in the research on these enzymes.
  • 2016From: Springer Protocols
    edited by Zdravka Medarova.
    Imaging functional nucleic acid delivery to skin / Roger L. Kaspar ... [et al.] -- In vivo magnetic resonance imaging of small interfering RNA nanodelivery to pancreatic islets / Ping Wang and Anna Moore -- Magnetic resonance spectroscopy of siRNA-based cancer therapy / Marie-France Penet ... [et al.] -- Targeted delivery with imaging assessment of siRNA expressing nanocassettes into cancer / Wei Chen and Lily Yang -- Analyses of tumor burden in vivo and metastasis ex vivo using luciferase-expressing cancer cells in an orthotopic mouse model of neuroblastoma / Frances L. Byrne, Joshua A. McCarroll, and Maria Kavallaris -- Indium-labeling of siRNA for small animal SPECT imaging / Steven Jones and Olivia Merkel -- Imaging of electrotransferred siRNA / Muriel Golzio and Justin Teissié -- Whole-body scanning PCR, a tool for the visualization of the in vivo biodistribution pattern of endogenous and exogenous oligonucleotides in rodents / Julien A. Boos and Iwan Beuvink -- siRNA nanoparticles for ultra-long gene silencing in vivo / Seung Koo Lee and Ching-Hsuan Tung -- Sensing miRNA : signal amplification by cognate RISC for intracellular detection of miRNA in live cells / Amol Kavishwar and Zdravka Medarova -- Molecular beacon-based microRNA imaging during neurogenesis / Jonghwan Lee and Soonhag Kim -- Hypoxia-responsive copolymer for siRNA delivery / Federico Perche ... [et al.] -- Controlling RNA expression in cancer using iron oxide nanoparticles detectable by MRI and in vivo optical imaging / Zdravka Medarova, Mustafa Balcioglu, and Mehmet V. Yigit -- Microvesicles : isolation, characterization for in vitro and in vivo procedures / Karmele Valencia and Fernando Lecanda -- Positive bioluminescence imaging of microRNA expression in small animal models using an engineered genetic-switch expression system, RILES / Patrick Baril and Chantal Pichon -- MicroRNA imaging in combination with diagnostic ultrasound and bubble liposomes for microRNA delivery / Yoko Endo-Takahashi ... [et al.].
  • 2011From: Springer
    edited by Lesley J. Collins.
    The RNA infrastructure : an introduction to ncRNA networks / Lesley J. Collins -- RNA interactions / Manja Marz and Peter F. Stadler -- Plant RNA silencing in viral defence / Vitantonio Pantaleo -- Micrornas as post-transcriptional machines and their interplay with cellular networks / Sarath Chandra Janga and Swathi Vallabhaneni -- Spatiotemporal aspects of microRNA-mediated gene regulation / Joris Pothof and Dik C. van Gent -- Spliceosomal RNA infrastructure : the network of splicing components and their regulation by miRNAs / Lesley J. Collins -- Construction, structure, and dynamics of post-transcriptional regulatory network directed by RNA binding proteins / Sarath Chandra Janga and Nitish Mittal -- Safe keeping the message : mRNP complexes tweaking after transcription / Said Hafidh, Věra Čapková, and David Honys -- Unexpected functions of tRNA and tRNA processing enzymes / Rebecca L. Hurto -- Programmed DNA elimination in tetrahymena : a small RNA mediated genome surveillance mechanism / Kensuke Kataoka and Kazufumi Mochizuki -- Long noncoding RNA and epigenomics / Chandrasekhar Kanduri -- Promoter associated long noncoding RNAs repress transcription through a RNA binding protein TLS / Riki Kurokawa -- RNA networks in prokaryotes I :CRISPRs and riboswitches / Patrick J. Biggs and Lesley J. Collins -- RNA networks in prokaryotes II : tRNA processing and small RNAs / Lesley J. Collins and Patrick J. Biggs -- Localization of the bacterial RNA infrastructure / Kenneth C. Keiler -- Small RNA discovery and characterisation in eukaryotes using high throughput approaches / Helio Pais ... [et al.] -- How old are RNA networks? / Toni Daly, X. Sylvia Chen, and David Penny.
  • 2005From: ScienceDirect
    edited by David R. Engelke, John J. Rossi.
    Also available: Print – 2005
  • 2008From: Springer
    Patrick J. Paddison, Peter K. Vogt, editors.
    RNA interference in mammalian cell systems / Patrick J. Paddison -- RNAi pathway in C. elegans: the argonautes and collaborators / Marie-Eve L. Boisvert and Martin J. Simard -- Genetics and biochemistry of RNAi in drosophila / Harsh H. Kavi ... [et al.] -- Role of dicer in posttranscriptional RNA silencing / Lukasz Jaskiewicz and Witold Filipowicz -- The mechanism of RNase III action: how dicer dices / Xinhua Ji -- MicroRNA metabolism in plants / Xuemei Chen -- Structure-function relationships among RNA-dependent RNA polymerases / Kenneth K.-S. Ng, Jamie J. Arnold, and Craig E. Cameron -- RNAi-mediated chromatin silencing in fission yeast / Sharon A. White and Robin C. Allshire -- A role for RNAi in heterochromatin formation in drosophila / Nicole C. Riddle and Sarah C.R. Elgin -- RNA mediated transcriptional gene silencing in human cells / Kevin V. Morris -- RNA silencing in mammalian oocytes and early embryos / Petr Svoboda -- Identifying human microRNAs / Isaac Bentwich.
    Also available: Print – 2008
  • 2015From: Springer Protocols
    edited by Mouldy Sioud.
    RNA interference : mechanisms, technical challenges, and therapeutic opportunities / Mouldy Sioud -- Nanostructured RNAs for RNA interference / Yuko Nakashima [and others] -- One long oligonucleotide or two short oligonucleotides based shRNA construction and expression / Xue-jun Wang and Sheng-qi Wang -- PLGA microspheres encapsulating siRNA / Giuseppe De Rosa and Giuseppina Salzano -- Magnetic nanoparticle and magnetic field assisted siRNA delivery in vitro / Olga Mykhaylyk [and others] -- Cytoplasmic delivery of siRNAs to monocytes and dendritic cells via electroporation / Mouldy Sioud -- Delivery of siRNAs to cancer cells via bacteria / Omar Ahmed, Andrea Krühn, and Hermann Lage -- Microwell array-mediated delivery of lipoplexes containing nucleic acids for enhanced therapeutic efficacy / Yun Wu, Daniel Gallego-Perez, and L. James Lee -- Modulating the tumor microenvironment with RNA interference as a cancer treatment strategy / Karin Zins [and others] -- Targeted in vivo delivery of siRNA and an endosome- releasing agent to hepatocytes / Magdolna G. Sebestyén [and others] -- Cell-internalization SELEX : method for identifying cell- internalizing RNA aptamers for delivering siRNAs to target cells / William H. Thiel [and others] -- Strategies for siRNA navigation to desired cells / Mouldy Sioud -- Use of guanidinopropyl-modified siRNAs to silence gene expression / Maximilian C.R. Buff [and others] -- Therapy of respiratory viral infections with intranasal siRNAs / Sailen Barik and Patrick Lu -- Engineering therapeutic cancer vaccines that activate antitumor immunity / Per Ole Iversen and Mouldy Sioud -- Immunosuppressive factor blockade in dendritic cells via siRNAs results in objective clinical responses / Mouldy Sioud, Anne Mobergslien, and Stein Sæbøe-Larssen -- Targeting bcr-abl transcripts with siRNAs in an imatinib- resistant chronic myeloid leukemia patient : challenges and future directions / Michael Koldehoff -- Facile method for interfering with off-target silencing mediated by the sense strand / Anne Mobergslien and Mouldy Sioud -- Overcoming the challenges of siRNA activation of innate immunity : design better therapeutic siRNAs / Mouldy Sioud -- Gene silencing in vitro and in vivo using intronic microRNAs / Jia Han Deng [and others] -- In silico identification of novel endo-siRNAs / Andrew Schuster [and others] -- Computer-assisted annotation of small RNA transcriptomes / Nicole Ortogero [and others] -- miR-CATCH : microRNA capture affinity technology / Sebastian Vencken [and others] -- Roles of MicroRNAs in cancers and development / Shigeru Takasaki -- Targeting microRNAs to withstand cancer metastasis / Valentina Profumo [and others] -- Urinary microRNAs as a new class of noninvasive biomarkers in oncology, nephrology, and cardiology / Hana Mlcochova [and others] -- Magnetic bead-based isolation of exosomes / Morten P. Oksvold, Axl Neurauter, and Ketil W. Pedersen -- Modified 2'-ribose small RNAs function as toll-like receptor-7/8 antagonists / Mouldy Sioud.
  • 2013From: Springer
    Kenneth A. Howard, editor.
    The Nobel Prize winning discovery that small interfering RNA can be utilised to control cellular gene expression has propelled the field of RNA interference (RNAi) to the forefront of biomedical science as a potential molecular medicine set to revolutionalise disease treatment. Harnessing the molecular mechanisms of RNAi and development of delivery technologies is crucial for its transformation into a therapeutic modality, this dependency is the focus of "RNA Interference from Biology to Therapeutics" that gives a comprehensive overview of RNAi biology and state-of-the-art delivery methods relevant to clinical translation of RNAi therapeutics. Key players and shapers in the fields of RNAi and delivery science have been assembled in a single volume to produce a truly unique interdisciplinary text, making it a "must-read" for both students and experts in, and at the interface of, RNAi, pharmaceutical science and medicine. An attractive feature is the "future perspectives" section within each chapter that allows global leaders the opportunity to express their views on the direction the field is moving. Topics covered in the book include miRNA biology and therapeutic exploitation, exosome delivery and clinical translation."RNA Interference from Biology to Therapeutics" is an up-to-the-minute, highly informative and invaluable text for those actively involved or interested in this fascinating and high-impact field.
  • 2011From: Springer Protocols
    edited by Scott Q. Harper.
    Designing hairpin-based RNAi shuttles / Yu Shen -- Rapid cloning and validation of microRNA shuttle vectors : a practical guide / Ryan L. Boudreau [and others] -- Use of small noncoding RNAs to silence transcription in human cells / Kevin V. Morris -- Delivery strategies for RNAi to the nervous system / Kevin D. Foust and Brian K. Kaspar -- Cloning small RNAs / Eric J. Devor and Lingyan Huang -- Profiling the miRNome : detecting global miRNA expression levels with DNA microarrays / Peter White -- High-throughput profiling of mature microRNA by real-time PCR / Jinmai Jiang [and others] -- In situ detection of microRNAs in paraffin-embedded, formalin-fixed tissues : different methodologies and co-localization with possible targets / Gerard J. Nuovo [and others] -- Tracing of siRNAs inside cells by FRET imaging / Markus Hirsch [and others] -- Application of RIP-chip for the identification of miRNA targets / Lu Ping Tan, Anke van den Berg, and Joost L. Kluiver.
  • 2005From: MyiLibrary
    edited by Krisharao Appasani ; forewords by Andrew Fire and Marshall Nirenberg.
    Chemical modifications to achieve increased stability and sensitive detection of siRNA -- RNA interference in postimplantation mouse embryos -- In ovo RNAi opens new possibilities for functional genomics in vertebrates -- Sect. 4. Gene silencing in model organisms. Practical applications of RNAi in C. elegans -- Inducible RNAi as a forward genetic tool in Trypanosoma brucei -- RNA-mediated gene silencing in fission yeast -- RNA silencing in filamentous fungi: Mucor ciccinelloides as a model organism -- RNAi and gene silencing phenomena mediated by viral suppressors in plants -- Sect. 1. Basic RNAi, siRNA, microRNAs and gene-silencing mechanisms. RNAi beginnings. Overview of the pathway in C. elegans -- Dicer in RNAi: its roles in vivo and utility in vitro -- Genes required for RNA interference -- MicroRNAs: a small contribution from worms -- miRNAs in the brain and the application of RNAi to neurons -- Sect. 2. Design, synthesis of siRNAs. Degign and synthesis of small interfering RNA (siRNA) -- Automated design and high throughput chemical synthesis of siRNA -- Rational design of siRNAs with the Sfold software -- Enzymatic production of small interfering RNAs -- Sect. 3. Vector development and in vivo, in vitro and in ovo delivery methods. Six methods of inducing RNAi in mammalian cells -- Viral delivery of shRNA -- siRNA delivery by lentiviral vectors: design and applications -- Liposomal delivery of siRNAs in mice -- Sect. 5. Drug target validation. Delivering siRNA in vivo for functional genomics and novel therapeutics -- The role of RNA interference in drug target validation: application to Hepatitis C -- RNAi in the drug discovery process -- RNA interference technology in the discovery and validation of druggable targets -- Sect. 6. Therapeutic and drug development. RNAi-mediated silencing of viral gene expression and replication -- RNAi in drug development: practical considerations -- RNA interference studies in liver failure -- RNAi applications in living animal systems -- Sect. 7. High-throughput genome-wide RNAi analysis. High-throughput RNAi by soaking in Caenorhabtis elegans -- Tools for integrative genomics: genome-wide RNAi and expression profiling in Drosophila -- Microarray analysis and RNA silencing to determine genes functionally important in mesothelioma -- High-throughput RNA interference -- Generation of highly specific vector-based shRNAi libraries directed against the entire human genome.
    Also available: Print – 2005
  • 2000From: ScienceDirect
    edited by Daniel W. Celander, John N. Abelson.
    Also available: Print – 2000
  • 2000From: ScienceDirect
    edited by Daniel W. Celander, John N. Abelson.
    Also available: Print – 2000
  • 2014From: Springer Protocols
    edited by M. Lucrecia Alvarez, Mahtab Nourbakhsh.
    Part I Structural RNA mapping -- Full-length characterization of transcribed genomic region -- Rapid mapping of RNA 3 and 5 ends -- Single nucleotide mapping of RNA 5 and 3 ends -- Analysis of RNA secondary structure -- Tertiary structure mapping of the Pri-miRNA miR-17-92 -- In situ hybridization detection of miRNA using LNA [trademark] oligonucleotides -- Quantification of miRNAs by a simple and specific qPCR method -- RNA isolation for small RNA next-generation sequencing from acellular biofluids -- Sequencing small RNA : introduction and data analysis fundamentals -- Measuring expression levels of small regulatory RNA molecules from body fluids and formalin-fixed, paraffin-embedded samples -- MicroRNA profiling in plasma or serum using quantitative RT-PCR -- MicroRNA profiling of exosomes isolated from biofluids and conditioned media -- Isolation of urinary exosomes for RNA biomarker discovery using a simple, fast, and highly scalable method -- Part II Functional RNA mapping -- Identification of actively translated mRNAs -- Mapping of internal ribosome entry sites (IRES) -- Mapping of protein binding RNA elements -- Purification of RNA-binding proteins -- De novo approach to classify protein-coding and noncoding transcripts based on sequence composition -- Computational methods to predict long noncoding RNA functions based on co-expression network -- MicroRNA biogenesis : dicing assay -- Faster experimental validation of microRNA targets using cold fusion cloning and a dual firefly-Renilla luciferase reporter assay -- Experimental validation of predicted mammalian microRNAs of mirtron origin -- A guide for miRNA target prediction and analysis using web-based applications -- Tapping microRNA regulation networks through integrated analysis of microRNA-mRNA high-throughput profiles -- miRWalk database for miRNA-target interactions -- A schematic workflow for collecting information about the interaction between copy number variants and microRNAs using existing resources -- SYBR green and TaqMan quantitative PCR arrays : expression profile of genes relevant to a pathway or a disease state -- Comprehensive meta-analysis of microRNA expression using a robust rank aggregation approach.
  • Yul Wonjun Yang.
    Various long noncoding RNAs (lncRNAs) have been recently identified, but their functions remain unknown. To better understand the role of lncRNAs in gene activation, we have characterized a 3.76 kilobase highly conserved lncRNA, called HOTTIP, found at the 5' distal tip of the HoxA locus. HOTTIP is required for the expression of HoxA9 to HoxA13 in human fibroblasts and chicken embryos. This region spans a linear 40 kilobases, but forms a compact three dimensional DNA structure by high throughput chromosome conformation capture. Knockdown of HOTTIP causes loss of the activating histone H3K4 trimethylation mark, as well as lost occupancy of the MLL methylase complex by chromatin immunoprecipitation coupled with tiling microarray analysis. To cause these changes, HOTTIP binds WDR5, a component of the MLL complex, to alter histone H3K4 trimethylation and gene activation. Together, the characterization of HOTTIP demonstrates an example of lncRNA-mediated epigenetic activation. A close examination of the binding interaction between HOTTIP and WDR5 has further revealed a novel pathway of lncRNA-regulated proteolysis. HOTTIP acts as a "molecular switch, " causing increased WDR5 protein levels by preventing proteasomal degradation through thermodynamic stabilization post poly-ubiquitination. Increased WDR5 deposition then causes gene activation. One HOTTIP RNA binds a single WDR5 protein through a direct RNA-protein interaction, and RNA-mediated stabilization requires a specific HOTTIP RNA domain in a long RNA context. Using a small scale alanine scanning mutagenesis screen, the HOTTIP binding interface on WDR5 has been identified as the cleft between blades 5 and 6. WDR5 mutations that abrogate lncRNA binding cannot be stabilized by HOTTIP, and are defective in gene activation, maintenance of histone H3K4 trimethylation, and embryonic stem cell self renewal. By altering protein turnover, lncRNAs may be able to regulate the temporal landscape of proteins in cells, potentially altering epigenetic states and cellular functions.
  • 2012From: Springer
    Albrecht Bindereif, editor.
    1. RNA polymerases and transcription factors of trypanosomes / Arthur Günzl -- 2. SL RNA biogenesis in kinetoplastids: a long and winding road / Nancy R. Sturm, Jesse R. Zamudio, and David A. Campbell -- 3. Pre-mRNA splicing in trypanosoma brucei: factors, mechanisms, and regulation / Christian Preusser, Nicolas Jaé, Arthur Günzl, and Albrecht Bindereif -- 4. mRNA turnover in trypanosomes / Christine Clayton -- 5. tRNA biogenesis and processing / Jessica L. Spears, Mary Anne T. Rubio, Paul J. Sample, and Juan D. Alfonzo -- 6. rRNA biogensis in trypanosomes / Shulamit Michaeli -- 7. RNA editing in African trypanosomes: a U-ser's G-U-ide / H. Ulrich Göringer -- 8. The RNA interference pathway in trypanosoma brucei / Elisabetta Ullu, Nikolay G. Kolev, Rebecca L. Barnes, and Christian Tschudi -- 9. Translation in trypanosomatids -- 10. Mitochondrial translation in trypanosomatids / Dmitri A. Maslov and Rajendra K. Agrawal -- 11. RNA-Seq analysis of the transcriptome of trypanosoma brucei / Jan Mani, Kapila Gunasekera, and Isabel Roditi.
  • 2010From: ScienceDirect
    Robert E. Farrell Jr.
    RNA and the cellular biochemistry revisited -- RNA isolation strategies -- The truth about tissues -- Going green : RNA and the molecular biology of plants -- Isolation of polyadenylated RNA -- Quality control for RNA preparations -- Resilient ribonucleases -- Stringency : conditions that influence nucleic acid structure -- Electrophoresis of RNA -- Photodocumentation and image analysis -- Northern analysis -- Nucleic acid probe technology -- Practical nucleic acid hybridization -- Principles of detection -- Quantifications of specific mRNAs by nuclease protection -- Analysis of nuclear RNA -- cDNA synthesis -- RT-PCR : a science and an art form -- Quantitative PCR techniques -- Functional genomics and transcript profiling -- High-throughput analysis of gene expression -- Non-array methods for global analysis of gene expression -- RNAi : take a RISC-role the dicer -- Genomes, transcriptomes, proteomes, and bioinformatics -- An RNA paradigm.
  • 2007From: ScienceDirect
    edited by Jonatha M. Gott.
    Also available: Print – 2007
  • 2015From: ScienceDirect
    edited by Chuan He.
    Methodology for the high-throughput identification and characterization of tRNA variants that are substrates for a tRNA decay pathway -- Nucleoside analysis by hydrophilic interaction liquid chromatography coupled with mass spectrometry -- A platform for discovery and quantification of modified ribonucleosides in RNA: application to stress-induced reprogramming of tRNA modifications -- Recogniion of specified RNA modifications by the innate immune system -- Kinetic analysis of tRNA methyltransferases -- Preparation of human nuclear RNA m6A methyltransferases and demethylases and biochemical characterization of their catalytic activity -- Transcriptome-wide mapping of N6-methyladenosine by m6A-Seq -- Probing RNA modification status at single-nucleotide resolution in total RNA -- High-resolution mapping of N6-methyladenosine in transcriptome and genome using a photo-crosslinking-assisted strategy -- Pseudouridine in mRNA: incorporation, detection, and recoding -- Pseudo-Seq: genome-wide detection of pseudouridine modifications in RNA -- Pseudouridine chemical labeling and profiling -- Experimental approaches for target profiling of RNA cytosine methyltranferases -- RNA 5-methylcytosine analysis by bisulfite sequencing -- Biochemical and transcriptome-wide identification of A-to-I RNA editing sites by ICE-Seq -- Radical SAM-mediated methylation of ribosomal RNA.
  • 2014From: CRCnetBASE
    edited by Peixuan Guo, Farzin Haque.
    Section 1. Introduction : principles and fundamentals of RNA nanotechnology -- section 2. RNA folding, structure, and motifs in RNA nanoparticle assembly -- section 3. RNA computation and structure prediction for RNA nanoparticle construction -- section 4. RNA chemistry for nanoparticle synthesis, conjugation, and labeling -- section 5. Single-molecule and biophysical techniques in RNA nanostructure analysis -- section 6. Methods for the assembly of RNA nanoparticles -- section 7. RNA nanoparticles for therapy of cancer, viral infections, and genetic diseases -- section 8. RNA nanotechnology for diagnostic applications -- section 9. Application of RNA aptamers in RNA nanotechnology and therapeutics -- section 10. Application of miRNAs in RNA nanotechnology and therapeutics -- section 11. Application of siRNAs in RNA nanotechnology and therapeutics.
  • 2015From: Springer Protocols
    edited by Peixuan Guo, Farzin Haque.
  • pt. C-D, 2003.From: ScienceDirect
    pt. DFrom: ScienceDirect
    edited by Sankar Adhya, Susan Garges.
    Also available: Print – pt. C-D, 2003.
  • 2016From: Springer
    Gene W. Yeo, editor.
    Experimental and computational considerations in the study of RNA binding protein-RNA interactions -- Genome-wide approaches for RNA structure probing -- Tethered function assays as tools to elucidate the molecular roles of RNA binding proteins -- Single molecule approaches in RNA-protein interactions -- RNA dynamics in the control of circadian rhythm -- Roles of RNA binding proteins and post-transcriptional regulation in driving male germ cell development in the mouse -- Regulation of stem cell self-renewal and oncogenesis by RNA binding proteins -- Controlling the editor: the many roles of RNA binding proteins in regulating A-to-I RNA editing -- Mutations in splicing factors and cancer -- Regulation of tissue-specific alternative splicing: C. elegans as a model system -- RNA Granules and Diseases -- A Case Study of Stress Granules in ALS and FTLD -- Post-translational modifications and RNA-binding proteins.
    Also available: Print – 2016
  • 1989From: ScienceDirect
    edited by James E. Dahlberg, John N. Abelson.
    Also available: Print – 1989
  • 1990From: ScienceDirect
    edited by James E. Dahlberg, John N. Abelson.
    Also available: Print – 1990
  • 2008From: Springer Protocols
    edited by Ren-Jang Lin.
    1. Isolation of a sequence-specific RNA binding protein, polypyrimidine tract binding protein using RNA affinity chromatography / Shalini Sharma -- 2. An affinity oligonucleotide displacement strategy to purify ribonucleoprotein complexes applied to human telomerase /Isabel Kurth, Gaël Cristofari, Joachim Lingner -- 3. RNA affinity tags for the rapid purification and investigation of RNAs and RNA-protein complexes / Scott C. Walker ... [et al.] -- 4. Assembly and glycerol gradient isolation of yeast spliceosomes containing transcribed or synthetic U6 SnRNA / Ken Dery, Shyue-Lee Yean, Ren-Jang Lin -- 5. Purification of ribonucleoproteins using peptide-elutable antibodies and other affinity techniques / Scott Stevens -- 6. CLIP: cross-linking and immunoprecipitation of in vivo RNA targets of RNA binding proteins / Kirk B Jensen, Robert B. Darnell -- 7. Quantitative analysis of protein-RNA interactions by gel mobility shift / Sean P. Ryder, Michael I. Recht, James R. Williamson -- 8. Monitoring assembly of ribonucleoprotein complexes by isothermal titration calorimetry / Michael I. Recht, Sean P. Ryder, James R. Williamson -- 9. Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome / A. Ozlem Tastan Bishop, ... [et al.] -- 10. In vivo analysis of ribonucleoprotein complexes using nucleotide analog interference mapping / Lara Weinstein Szewczak -- 11. T7 RNA polymerase mediated incorporation of 8-N3AMP into RNA for studying protein-RNA interactions / Rajesh Gaur -- 12. A simple crosslinking method, CLAMP, to map the sites of RNA contacting domains within a protein / Hiren Banerjee, Ravinder Singh -- 13. Proteins specifically modified with a chemical nuclease as probes of RNA-protein interaction / Oliver A. Ken, Andrew M. MacMillan -- 14. RNA-protein crosslink mapping using TEV protease / Ian A. Turner, ... [et al.] -- 15 Structural analysis of protein-RNA interactions with mass spectrometry / Mamuka Kvaratskhelia, Stuart F.J. Le Grice -- 16. Analysing RNA-protein cross-linking sites in unlabeled ribonucleoprotein complexes by mass spectrometry / Henning Urlaub, Eva Kühn-Hölsken, Reinhard Lührmann -- 17. In vitro selection of random RNA fragments to identify protein binding sites within large RNAs / Ulrich Stelzl, Knud H. Nierhaus -- 18. Immunoprecipitation analysis to study RNA-protein interactions in xenopus oocytes / Naoto Mabuchi, Kaoru Masuyama, Mutsuhito Ohno -- 19. Mapping the regions of RNase P catalytic RNA that are potentially in close contact with its protein cofactor / Phong Trang, Fenyong Liu -- 20. Quantification of microRNAs, splicing isoforms, and homologous mRNAs with the invader assay / Peggy S. Eis, Mariano Garcia-Blanco -- 21. Analysis of RNA structure and RNA-protein interactions in mammalian cells by use of terminal transferase-dependent PCR / Hsiu-Hua Chen, Jeanne LeBon, Arthur D. Riggs -- 22. Duplex unwinding and RNP remodeling with RNA helicases / Eckhard Jankowsky, Margaret E. Fairman -- 23. Preparation of efficient splicing extracts from whole cells, nuclei, and cytoplasmic fractions / Naoyuki Kataoka, Gideon Dreyfuss -- 24. Designing and utilization of siRNAs targeting RNA-binding proteins / Dong-Ho Kim, Mark Behlke, John J. Rossi -- 25. The use of saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins / Jane E. Jackman, Eric M. Phizicky.
  • 2015From: Springer Protocols
    edited by Marc Boudvillain.
  • 2015From: Springer Protocols
    edited by Frank J. Schmidt.
    Identifying RNA recombination events and non-covalent RNA-RNA interactions with the molecular colony technique / Helena V. Chetverina and Alexander B. Chetverin -- RNA-directed recombination of RNA in vitro / Niles Lehman, Nilesh Vaidya, and Jessica A.M. Yeates -- RNA-RNA SELEX / B. Cho -- Identification of antisense RNA stem-loops that inhibit RNA-protein interactions using a bacterial reporter system / Kazuo Harada -- Transactivation of large ribozymes / Matthew B. Martin, Thomas L. Leeper, and Frank J. Schmidt -- Native purification and labeling of RNA for single molecule fluorescence studies / Arlie J. Rinaldi, Krishna C. Suddala, and Nils G. Walter -- Single molecule studies of RNA-RNA interactions / Dongmei Yu, Peiwu Qin, and Peter V. Cornish -- Modification interference analysis of the ribosome / Simpson Joseph -- Assessing intermolecular RNA:RNA interactions within a ribonucleoprotein complex using heavy metal cleavage mapping / Keith T. Gagnon and E. Stuart Maxwell -- Electrophoretic mobility shift assays : analysis of tRNA binding to the T box riboswitch antiterminator RNA / R. Anupam, S. Zhou, and J.V. Hines -- Fluorescence anisotropy : analysis of tRNA binding to the T box riboswitch antiterminator RNA / S. Zhou, R. Anupam, and J.V. Hines -- Electrophoretic mobility shift assay of RNA-RNA complexes / Geunu Bak [and three others] -- Structural studies of a double-stranded RNA from trypanosome RNA editing by small-angle X-ray scattering / Angela Criswell and Blaine H.M. Mooers -- Fusion RNAs in crystallographic studies of double-stranded RNA from trypanosome RNA editing / Blaine H.M. Mooers.
  • 2015From: Springer Protocols
    edited by Luc Ponchon.
    Method to predict the 3D structure of an RNA scaffold / Xiaojun Xu and Shi-Jie Chen -- Post-crystallization improvement of RNA crystal diffraction quality / Jinwei Zhang and Adrian R. Ferré-D'Amaré -- Expression and purification of RNA-protein complexes in Escherichia coli / Margueritte El Khouri and others -- Production of homogeneous recombinant RNA using a tRNA scaffold and hammerhead ribozymes / Frank H.T. Nelissen, Hans A. Heus, and Sybren S. Wijmenga -- In vivo production of small recombinant RNAs embedded in a 5S rRNA-derived protective scaffold / Victor G. Stepanov and George E. Fox -- Detection of RNA-protein interactions using tethered RNA affinity capture / Hidekazu Iioka and Ian G. Macara -- Universal method for labeling native RNA in live bacterial cells / Irina Smolina and Natalia Broude -- Live cell imaging using riboswitch-spinach tRNA fusions as metabolite-sensing fluorescent biosensors / Colleen A. Kellenberger, Zachary F. Hallberg, and Ming C. Hammond -- RNA scaffold : designed to co-localize enzymes / ZJU_China Team (iGEM 2012) and Ming Chen -- Artificial ligase ribozymes isolated by a "Design and Selection" strategy / Shigeyoshi Matsumura and Yoshiya Ikawa -- Engineering aptazyme switches for conditional gene expression in mammalian cells utilizing an in vivo screening approach / Charlotte Rehm, Benedikt Klauser, and Jörg S. Hartig -- Aptazyme-based riboswitches and logic gates in mammalian cells / Yoko Nomura and Yohei Yokobayashi -- Design and characterization of topological small RNAs / Jack Hassall and others -- Folding RNA-protein complex into designed nanostructures / Tomonori Shibata and others -- Simple method for constructing RNA triangle, square, pentagon by tuning interior RNA 3WJ angle from 60° to 90° or 108° / Emil F. Khisamutdinov and others -- RNA-mediated CdS-based nanostructures / Vinit Kumar and Anil Kumar -- Effective method for specific gene silencing in Escherichia coli using artificial small RNA / Geunu Bak and others.
  • 2014From: Springer Protocols
    edited by Jan Gorodkin, Walter L. Ruzzo.
    The existence of genes for RNA molecules not coding for proteins (ncRNAs) has been recognized since the 1950's, but until recently, aside from the critically important ribosomal and transfer RNA genes, most focus has been on protein coding genes. However, a long series of striking discoveries, from RNA's ability to carry out catalytic function, to discovery of riboswitches, microRNAs and other ribo-regulators performing critical tasks in essentially all living organisms, has created a burgeoning interest in this primordial component of the biosphere. However, the structural characteristics and evolutionary constraints on RNA molecules are very different from those of proteins, necessitating development of a completely new suite of informatic tools to address these challenges. In RNA Sequence, Structure, Function: Computational and Bioinformatic Methods, expert researchers in the field describe a substantial and relevant fraction of these methodologies from both practical and computational/algorithmic perspectives. Focusing on both of these directions addresses both the biologist interested in knowing more about RNA bioinformatics as well as the bioinformaticist interested in more detailed aspects of the algorithms. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results. Thorough and intuitive, RNA Sequence, Structure, Function: Computational and Bioinformatic Methods aids scientists in continuing to study key methods and principles of RNA bioinformatics.
  • 2010From: Springer
    Volker A. Erdmann, Jan Barciszewski, editors.
    The key features of RNA silencing / Kuniaki Saito ... [et al.] -- Selected strategies for the delivery of siRNA in vitro and in vivo / Sandra D. Laufer ... [et al.] -- RNAi suppression and its application / Xiaoping Yi, Rui Lu -- Strategies to prevent siRNA-triggered cellular toxicity / Matthias Bauer -- RNAi in malignant brain tumors: relevance to molecular and translational research / Mitsutoshi Nakada ... [et al.] -- Silencing Huntington's disease gene with RNAi / Yu Zhang, Robert M. Friedlander -- Application of Dicer-substrate siRNA in pain research / Philippe Sarret ... [et al.] -- RNAi treatment of HIV-1 infection / Karin J. von Eije, Ben Berkhout -- Application of RNA interference to treat conditions associated with dysregulation of transient receptor potential vanilloid 1 channel / Vickram Ramkumar ... [et al.] -- Harnessing RNAi-based functional genomics to unravel the molecular complexity underlying skin pigment variation / Hsiang Ho ... [et al.] -- mRNA structure and its effects on posttranscriptional gene silencing / Stephen I. Rudnick, Veenu Aishwarya, Alan M. Gewirtz -- Antisense RNA-mediated regulation of the p53 tumor suppressor / Marianne Farnebo, Klas G. Wiman -- Antisense oligonucleotides: insights from preclinical studies and clinical trials / Doreen Kunze, Kai Kraemer, Susanne Fuessel -- What can the new hammerhead ribozyme structures teach us about design? / William G. Scott -- microRNA biogenesis and its impact on RNA interference / Stefanie Grund, Sven Diederichs -- MicroRNAs in epithelial antimicrobial immunity / Jun Liu ... [et al.] -- Emerging roles of long noncoding RNAs in gene expression and intracellular organization / Tetsuro Hirose -- Noncoding RNAs as therapeutic targets / Maciej Szymański, Jan Barciszewski -- Noncoding RNAs at H19/IGF2 locus: role in imprinting, gene expression, and associated pathologies / Nahalie Berteaux, Nathalie Spruyt, Eric Adriaenssens.
  • 2008From: Springer
    edited by Volker A. Erdmann, Wolfgang Poller, Jan Barciszewski.
  • 2006From: Springer
    contributors: A. Adler ... [et al.] ; editors: Volker Erdmann, Jürgen Brosius and Jan Barciszewski.
  • 2008From: ScienceDirect
    edited by Lynne E. Maquat, Cecilia M. Arraiano.
    Also available: Print – 2008
  • 2008From: ScienceDirect
    edited by Lynne E. Maquat, Megerditch Kiledjian.
    Also available: Print – 2008
  • 2008From: ScienceDirect
    edited by Lynne E. Maquat, Megerditch Kiledjian.
    Also available: Print – 2008
  • 2008From: Springer Protocols
    edited by Sailen Barik.
  • 2011From: Springer Protocols
    edited by Hiroaki Kodama, Atsushi Komamine.
    Overview of plant RNAi / Yuichiro Watanabe -- Caveat of RNAi in plants : the off-target effect / Muthappa Senthil-Kumar and Kirankumar S. Mysore -- Plant gateway vectors for RNAi as a tool for functional genomic studies / Toshiya Muranaka -- Heat-inducible RNAi for gene functional analysis in plants / Frederic Masclaux and Jean-Philippe Galaud -- Gene function analysis by artificial microRNAs in Physcomitrella patens / Basel Khraiwesh [and others] -- Virus-induced gene silencing in ornamental plants / Cai-Zhong Jiang, Jen-Chih Chen, and Michael Reid -- Local RNA silencing mediated by agroinfiltration / Jutta Maria Helm, Elena Dadami, and Kriton Kalantidis -- Direct transfer of synthetic double-stranded RNA into protoplasts of Arabidopsis thaliana / Ha-il Jung, Zhiyang Zhai, and Olena K. Vatamaniuk -- Detection of long and short double-stranded RNAs / Toshiyuki Fukuhara [and others] -- Quantitative stem-loop RT-PCR for detection of microRNAs / Erika Varkonyi-Gasic and Roger P. Hellens -- Large-scale sequencing of plant small RNAs / William P. Donovan, Yuanji Zhang, and Miya D. Howell -- Computational prediction of plant miRNA targets / Ying-Hsuan Sun [and others] -- Bisulfite sequencing for cytosine-methylation analysis in plants / Nazmul Haque and Masamichi Nishiguchi -- Using nuclear run-on transcription assays in RNAi studies / Basel Khraiwesh -- Proteomic analysis of RNA interference induced knockdown plant / Sang Yeol Lee and Kyun Oh Lee -- Comparative analysis of phosphoprotein expression using 2D-DIGE / Tomoya Asano and Takumi Nishiuchi.
  • 2011From: CRCnetBASE
    editors, R.K. Gaur, Yedidya Gafni, P. Sharma, V.K. Gupta.
    "RNA interference (RNAi) is a system within living cells that helps to control active genes and their activity level. Two types of small RNA molecules--microRNA (miRNA) and small interfering RNA (siRNA)--are central to RNA interference. The discovery of RNAi was preceded first by observations of transcriptional inhibition by antisense RNA expressed in transgenic plants and more directly by reports of unexpected outcomes in experiments performed by plant scientists in the U.S. and The Netherlands in the early 1990s. RNAi technology are used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. This book reviews the selective and robust effect of RNAi on gene expression which makes it a valuable research tool, both in cell culture and in living organisms because synthetic dsRNA introduced into cells can induce suppression of specific genes of interest. This book will introduce new technology in the study of RNA interference in microorganisms, plants and animals."--Provided by publisher.
  • Ho Lun Wong.
    The overall objective of this dissertation is to evaluate the impacts of three development programs in rural China. Specifically, I examine the impacts of investment into: a.) village infrastructure; b.) children health; and c.) children education in China's rural villages. To better understand how such investments can best be made, I proceed by writing three papers. In the first paper I seek to answer the question of how to build high quality and cost effective infrastructure in China's rural villages. I attempt to address a fundamental question of infrastructure investment--who is better at infrastructure investment. Should the village leadership or a government agency above the village finance and/or manage infrastructure projects? To answer this question, I take advantage of China's ambitious rural road building agenda in the recent years. My collaborators surveyed all road projects in 101 villages in rural China from 2003 to 2007. In the survey the quality and cost per kilometer of each road was measured. According to the analysis, road quality was higher when more of the project finance came from the government agency. Moreover, projects had lower costs per kilometer when the village leadership participated in the project's management. Overall, my findings suggest that to build high quality and cost effective roads, government agencies should finance/design road projects and the village leadership should participate in project management. In the second paper I report on the results of a randomized controlled trial that was conducted among over 2,000 children in 60 elementary schools in rural Shaanxi Province, Northwest China. I examine the effects of two separate interventions--a parental education treatment and an iron supplementation treatment--on the anemia status and educational performance of children in elementary schools in rural China. I find that providing children with daily iron supplements for six months improved the hemoglobin (Hb) levels and math test scores of children. The effects of the supplement treatment were larger among children who lived and boarded at home relative to children who lived and boarded at schools. In comparison, educating parents about nutrition and anemia only improved the Hb levels of non-boarding children and only raised the test scores of non-boarding children who were also baseline-anemic. In the third paper, I attempt to understand why preschool attendance in poor rural areas of China is low when preschool has been shown (internationally) to improve children's school readiness in many developing countries. To examine this question, I evaluate the impact of a voucher/conditional cash transfer (CCT) program, which is one possible policy intervention that the Chinese government is considering to increase attendance. The goal of the intervention is to help parents defray the cost of preschool on preschool attendance and raise school readiness. To meet this goal, I helped to conduct a (individual-level) randomized controlled trial among 150 children in a poor, rural county in China. The analysis shows that a one-year voucher/CCT intervention, consisting of a tuition waiver and a cash transfer conditional on attendance, raised attendance by 20 percentage points (or by 35 percent). However, the intervention did not have measurable impact on the school readiness of students. Based on these findings, I propose that one potential explanation for these findings is the poor quality of preschool education in rural China.
  • 2015From: ClinicalKey
    Edward C. Klatt.
    Blood vessels -- The heart -- Hematopathology -- Red blood cell disorders -- The lung -- Head and neck -- The gastrointestinal tract -- The liver and biliary tract -- The pancreas -- The kidney -- The lower urinary tract -- The male genital tract -- The female genital tract -- The breast -- The endocrine system -- The skin -- Bones, joints, and soft tissue tumors -- Peripheral nerve and skeletal muscle -- The central nervous system -- The eye.
  • 2015From: ClinicalKey
    [edited by] Vinay Kumar, Abul K. Abbas, Jon C. Aster ; with illustrations by James A. Perkins.
    Also available: Print – 2015
  • [edited by] Vinay Kumar, Abul K. Abbas, Nelson Fausto ; with illustrations by James A. Perkins.
    PrintStatus: Not Checked OutLane Catalog Record
    Unit I General pathology -- Ch. 1 Cellular adaptations, cell injury, and cell death -- Ch. 2 Acute and chronic inflammation -- Ch. 3 Tissue renewal and repair: regeneration, healing and fibrosis -- Ch. 4 Hemodynamic disorders, thromboembolic disease, and shock -- Ch. 5 Genetic disorders -- Ch. 6 Diseases of immunity -- Ch. 7 Neoplasia -- Ch. 8 Infectious diseases -- Ch. 9 Environmental and nutritional pathology -- Ch. 10 Diseases of infancy and childhood -- Unit II Diseases of organ systems -- Ch. 11 Blood vessels -- Ch. 12 The heart -- Ch. 13 Red blood cell and bleeding disorders -- Ch. 14 Diseases of white blood cells, lymph nodes, spleen, and thymus -- Ch. 15 The lung -- Ch. 16 Head and neck -- Ch. 17 The gastrointestinal tract -- Ch. 18 Liver and binary tract -- Ch. 19 The pancreas -- Ch. 20 The kidney -- Ch. 21 The lower urinary tract and male genital system -- Ch. 22 The female genital tract -- Ch. 23 The breast -- Ch. 24 The endocrine system -- Ch. 25 The skin -- Ch. 26 Bones, joints, and soft tissue tumors -- Ch. 27 Peripheral nerve and skeletal muscle -- Ch. 28 The central nervous system -- Ch. 29 The eye -- Index.
  • [edited by] Vinay Kumar, Abul K. Abbas, Nelson Fausto, Jon C. Aster ; with illustrations by James A. Perkins.
    PrintStatus: Not Checked OutLane Catalog Record
  • Edward C. Klatt, Vinay Kumar.
    PrintStatus: Not Checked OutLane Catalog Record
  • 2018From: ClinicalKey
    [edited by] Vinay Kumar, Abul K. Abbas, Jon C. Aster ; artist, James A. Perkins.
    The cell as a unit of health and disease / Richard. N. Mitchell -- Cell injury, cell death, and adaptations -- Inflammation and repair -- Hemodynamic disorders, thromboembolism, and shock -- Diseases of the immune system -- Neoplasia -- Genetic and pediatric diseases / Anirban Maitra -- Environmental and nutritional diseases -- General pathology of infectious diseases / Alexander J. McAdam, Karen M. Frank -- Blood vessels / Richard N. Mitchell -- Heart / Richard N. Mitchell -- Hematopoietic and lymphoid systems -- Lung / Aliya Noor Husain -- Kidney and its collecting system / Anthony Chang, Zoltan Laszik -- Oral cavity and gastrointestinal tract / Jerrold R. Turner, Mark W. Lingen -- Liver and gallbladder / Neil D. Theise -- Pancreas / Anirban Maitra -- Male genital system and lower urinary tract / Jonathan Epstein, Tamara L. Lotan -- Female genital system and breast / Lora Hedrick Ellenson, Susan C. Lester -- Endocrine system / Anirban Maitra -- Bones, joints, and soft tissue tumors / Andrew Horvai -- Peripheral nerves and muscles / Peter Pytel -- Central nervous system / Matthew P. Frosch -- Skin / Alexander J.F. Lazar.
  • Vinay Kumar, Abul K. Abbas, Nelson Fausto, Richard N. Mitchell.
    PrintStatus: Not Checked OutLane Catalog Record
    Cell injury, cell death, and adaptations -- Acute and chronic inflammation -- Tissue repair : regeneration, healing, and fibrosis -- Hemodynamic disorders, thrombosis, and shock -- Diseases of the immune system -- Neoplasia / Thomas P. Stricker, Vinay Kumar -- Environmental and nutritional diseases -- General pathology of infectious diseases -- The blood vessels -- The heart / Frederick J. Schoen, Richard N. Mitchell -- The hematopoietic and lymphoid systems / Jon C. Aster -- The lung / Anirban Maitra, Vinay Kumar -- The kidney and its collecting system / Charles E. Alpers , Agnes B. Fogo -- The oral cavity and gastrointestinal tract -- The liver, gallbladder, and biliary tract -- The pancreas -- The male genital system -- The female genital system / Anthony Montag, Vinay Kumar -- The endocrine system / Anirban Maitra -- The musculoskeletal system -- The skin / Alexander J.F. Lazar -- The nervous system / Matthew P. Frosch.
  • 2013From: ClinicalKey
    editor-in-chief, James R. Roberts ; senior editor, Catherine B. Custalow ; illustration editor, Todd W. Thomsen ; editor emeritus, Jerris R. Hedges.
    Vital signs and patient monitoring techniques -- Respiratory procedures -- Cardiac procedures -- Vascular techniques and volume support -- Anesthetic and analgesic techniques -- Soft tissue procedures -- Gastrointestinal procedures -- Musculoskeletal procedures -- Genitourinary, obstetric, and gynecologic procedures -- Neurologic procedures -- Ophtalmologic, otolaryngologic, and dental procedures -- Special procedures.
  • 2016From: Springer
    John W. Davis, editor.
    Minimally invasive access to the prostate -- The concept of surgical space creation -- Surgeon's Notebook: Beyond the Learning Curve: The Original Handouts from the American Urological Association Post-graduate Courses 2013-2015 -- The Dorsal Vein Complex -- Achieving Hemostasis and Proper Set-up for Apical Division -- The Simple Bladder Neck -- Bladder Neck -- Anatomic Variants, Prior TUR, Locally Advanced Tumors -- Neurovascular Bundle Preservation: Anatomic and Technical Considerations -- Partial Neurovascular Bundle Sparing during Robot-Assisted Radical Prostatectomy -- Neurovascular Structure-adjacent Frozen-section Examination (NeuroSAFE) in robot-assisted radical prostatectomy -- Neurovascular Bundle -- Wide Excision -- Apex -- The Crossroads of Functional Recovery and Oncologic Control -- Post-Resection: Hemostasis, Checking for Rectal Injury, Anastomotic Leaks -- Denonvilliers Fascia -- Anatomy, Surgical Planes, Use in Reconstruction -- Vesico-urethral Anastomosis -- Putting it Back Together -- Pelvic Lymph Node Dissection -- Open Benchmarks with Lymphoscintigraphy -- Pelvic Lymph Node Dissection -- Robotic Surgery Efficiency and Space-Creation Techniques to Achieve an Extended Template -- Innovation and Orientation Challenges -- Posterior "Retzius Sparing" Technique -- Getting Out -- Closure, Prevention and Management of Hernias -- Robot-Assisted Radical Prostatectomy Extraperitoneal and Transperitoneal Techniques -- Perioperative Medical Evaluation of the Patient undergoing RARP -- Patient Positioning and Safety in Steep Trendelenburg -- Safety checklist for training and assessment in robot assisted prostate surgery -- Immediate Post-Operative Care Following Robot Assisted Radical Prostatectomy -- Penile Rehabilitation: Recovering Erectile Function following Prostate Cancer Treatment -- Structured Reporting of RARP Complications -- Are We Making Measurable Progress? -- Quality of Life Evaluation: What is Published and Practical for Routine Use -- Positive Surgical Margins: How to Self-Evaluate and Improve -- High Risk Pathology: State-of-the-Art Post-Operative Radiation Recommendations and Integration of Novel Genomic Risk Biomarkers -- Long-term Oncologic Outcomes -- Personalized versus Generic Patient Handouts -- Tools to Improve Patient Knowledge on Treatment Options and Peri-operative Care -- Active Surveillance and Patient Support Intervention -- A Return to Key Surgical Technique: Neurovascular Bundle Sparing with Antegrade Technique.
  • 2011From: Springer
    edited by Michael C. Ost.
    Laparoscopic and robotic instrumentation for urologic reconstructive surgery in adults -- Instrumentation during pediatric robotic anastomoses and reconstruction -- Instrumentation during pediatric laparoscopic robotic anastomoses and reconstruction -- Adult laparoscopic partial nephrectomy for renal cell carcinoma -- Adult robotic-assisted partial nephrectomy for renal cell carcinoma -- Pediatric laparoscopic and robotic upper pole nephrectomy for nonfunctioning moieties -- Adult laparoscopic and robotic-assisted pyeloplasty for ureteropelvic junction obstruction -- Pediatric laparoscopic (infant, pre-pubertal, and teenager) pyeloplasty for ureteropelvic junction obstruction -- Pediatric robotic (infant, pre-pubertal, and teenager) pyeloplasty for ureteropelvic junction obstruction -- Laparoscopic ureteroureterostomy and correction of ureteral defects -- Robotic ureteroureterostomy and correction of ureteral defects -- Robotic radical cystectomy and use of intestinal segments for reconstruction in the adult patient -- Laparoscopic bladder augmentation and creation of continent-catheterizable stomas in the pediatric patient -- Laparoscopic ureteral reimplant surgery to correct reflux disease -- Robotic ureteral reimplant surgery to correct reflux disease -- Laparoscopoic and robotic orchiopexy for the impalpable undescended testicle -- Laparoscopic anastomoses and bladder neck reconstruction following radical prostatectomy -- Robotic anastomoses and bladder neck reconstruction following radical prostatectomy -- The role of NOTES and LESS in minimally invasive reconstructive urological surgery -- Laparoscopy and robotics in stress urinary incontinence and pelvic reconstructive surgery.
  • 2015From: Springer
    Howard Ross, Sang W. Lee, Bradley J. Champagne, Alessio Pigazzi, David E. Rivadeneira, editors.
  • 2017From: CRCnetBASE
    edited by Pedro Encarnação and Albert M. Cook.
    1. Fundamentals of Robotic Assistive Technologies -- 2. Human-Robot Interaction for Rehabilitation Robots -- 3. Assistive Robotic Manipulators -- 4. Upper and Lower Limb Robotic Prostheses -- 5..Smart Wheelchairs for Assessment and Mobility -- 6. Exoskeletons as an Assistive Technology for Mobility and Manipulation -- 7. Robotic Systems for Augmentative Manipulation to Promote Cognitive Development, Play, and Education -- 8. Social Assistive Robots for Children with Complex Disabilities -- 9. Robots Supporting Care for Elderly People -- 10. Ethical and Social Implications of the Use of Robots in Rehabilitation Practice.
  • 2014From: Springer
    Changqing Gao, editor.
    Robotic Cardiac Surgery is a comprehensive guide to robotic/totally endoscopic cardiac surgery. The book is intended to provide in-depth information regarding the history of robotic surgical systems, their components and principles. It emphasizes patient selection, perioperative management, anesthesia considerations and management, operative techniques and management, postoperative care and results. Extensive, detailed photographs and illustrations of different kinds of robotic surgery are also included. It provides cardiac surgeons, cardiac anesthesiologists, and perfusionists with a comprehensive review of current robotic cardiac surgeries and related knowledge.
  • 2014From: Springer
    Said Abdallah, Al-Mamari, Hervé Quintens, editors.
    Robotic Donor Nephrectomy - A Practical Guide is a user friendly manual aimed to give both novice and mature urologists a clear and comprehensive description of robot-assisted laparoscopic donor nephrectomy through a step-by-step approach. Novel techniques such as the Laparo-Endoscopic Single-Site (LESS) surgery are examined and the editors also look at the well established multi-ports techniques using the da Vinci Surgical system. Robotic Donor Nephrectomy - A Practical Guide will help young urologists gain better understanding of the techniques of robotic-assisted surgery, and support older urologists in 'crossing the border' towards laparoscopic surgery, particularly for delicate procedures such as the donor nephrectomy.
  • 2013From: Springer
    Ronney Abaza, editor.
    Part I: Robotic Technology and Instrumentation -- Robotic Technology and Ancillary Instruments in Renal Surgery -- Preoperative Preparation and Positioning for Robotic Renal Surgery -- Part II: Nephrectomy -- Robotic Simple and Radical Nephrectomy -- Robotic Donor Nephrectomy: Hand-Assisted Technique -- Robotic Nephrectomy for Complex Tumors -- Part III: Partial Nephrectomy -- Robotic Partial Nephrectomy: Basic Principles and Techniques -- Robot-Assisted Partial Nephrectomy: Complex Tumors and Evolving Techniques -- Near-Infrared Fluorescence Imaging in Robotic Partial Nephrectomy -- Part IV: Upper Urinary Tract Surgery -- Robotic Pyeloplasty -- Robotic Nephroureterectomy -- Robotic Ureteral Resection and Reconstruction for Urothelial Tumors and Benign Disease -- Part V: Pediatric Robotic Renal and Ureteral Surgery -- Upper Tract Obstruction -- Vesicoureteral Reflux and Ureteral Reimplantation -- Part VI: Advanced and Evolving Robotic Renal Techniques -- Retroperitoneal Robotic Kidney Surgery -- Single-Incision Robotic Renal and Ureteral Surgery.
  • 2014From: Springer
    Go Watanabe, editor.
    1. Overview of robotic surgery -- 2. The de Vinci Surgical® systesm -- 3. Development of robotic systems -- 4. Robotic surgery in urology -- 5. Robotic gastrectomy for gastric cancer -- 6. Esophageal cancer surgery: robotic esophagectomy - 7. Lateral pelvic node dissection for advanced rectal cancer: current debates and use of the robotic approach -- 8. Cardiac surgery: overview -- 9. Robotically assisted totally endoscopic coronary artery bypass grafting -- 10. Robotic surgery for mitral valve disease -- 11. Robotic surgery in general thoracic surgery -- 12. Robot-assisted thyroidectomy.
  • 2015From: Springer
    Giuseppe Spinoglio, editor ; in collaboration with Alessandra Marano and Giampaolo Formisano ; forewords by Giorgio De Toma, Francesco Corcione.
    This book describes the current applications of the robotic system in general surgery, focusing on the technical aspects of the procedures most commonly performed by this means. It provides a comprehensive overview of the state of the art in robotic surgery and presents the most recently available evidence as documented in the literature. The opening chapters review robotic platforms, discuss the general advantages, limitations, and strategies of robotic surgery, and identify challenges and critical elements when setting up a robotic program. The full range of applications of the robotic system is then covered, encompassing thoracic, esophageal, gastric, hepatobiliopancreatic, splenic, colorectal, endocrine, and transplantation surgery. Detailed attention is also paid to innovative applications and future trends in the robotic platform, with inclusion of a special chapter on fluorescence imaging during robotic procedures. The book closes by considering aspects related to credentialing and privileging, such as mentoring, proctoring, and teaching modules for residents and fellows. Against the background of increasing acceptance of the robotic surgery system across the globe, this book will be invaluable for all general surgeons. There is no doubt that within general surgery the system will continue to flourish on account of its undoubted technical advantages, which minimize most of the intrinsic shortcomings of laparoscopy.
  • 2014From: Springer
    Erik P. Castle, Raj S. Pruthi, editors.
    Robotic Surgery of the Bladder is a dedicated resource to understanding and mastering the concepts and practical aspects of robotic bladder surgery. The volume provides an introduction to urologists interested in initiating robotic radical cystectomy by providing them background, concepts, steps, and tools and tricks to introduce robotic radical cystectomy into their clinical practice, all in a safe, stepwise, and organized manner. For the more experienced robotic surgeon, the text serves as a resource for more advanced applications and techniques. The volume is also accompanied by many still images and a DVD of video clips. Written by experts in the field, Robotic Surgery of the Bladder is a comprehensive resource that maintains the time-tested oncologic principles of bladder cancer surgery, while introducing the advantages that exist with robotic-assisted approaches.
  • 2015From: Springer
    Gregory A. Grillone and Scharukh Jalisi.
    Robotic Surgery of the Head and Neck is the first comprehensive guide for otolaryngologists who wish to perform robotic head and neck surgery. Edited by leaders in the field, this book focuses on how improved access, visualization, and flexibility of the technology have greatly expanded the capabilities of the head and neck surgeon to treat diseases transorally or through small incisions in the skin. Starting with an overview of minimally invasive surgery in the head and neck, and moving to discussions of anatomic considerations for these procedures and the future applications of robotic surgery for otolaryngologists, Robotic Surgery of the Head and Neck explores the exciting progress of robotic technologies, bringing physicians closer to achieving the benefits of traditional surgery with the least amount of disruption to the patient.
  • Jeffrey Steven Schlosser.
    External Beam Radiation Therapy (EBRT) is used in the disease management of more than half of all cancer patients. In order to improve clinical EBRT outcomes, highly conformal, potent ablative doses must be delivered to maximize local tumor control and minimize toxicity to surrounding healthy tissue. Random and quasi-periodical anatomy motion during beam delivery poses a fundamental threat to realizing such conformality, and thus restricts the curative potential of EBRT. We address this problem in the development and evaluation of a novel robotic ultrasound guidance system that integrates with existing medical linear accelerators (LINACs), enabling real-time, volumetric, non-invasive, non-ionizing, markerless tracking of soft tissue targets during radiation delivery. Major challenges in the deployment of robotic ultrasound guidance for radiotherapy include image acquisition, image calibration, tissue tracking, and treatment planning. For image acquisition, we first present a novel robotic manipulator designed to perform two-dimensional (2D) prostate ultrasound imaging while minimizing interferences with the radiation delivery system. A second, more generalized robotic manipulator is then presented, designed for 3D ultrasound imaging of multiple abdominal sites. We demonstrate stable remote ultrasound image acquisition with each robot over extended time periods in volunteers. Ultrasound image calibration is required to reconstruct image data in the LINAC frame for treatment guidance. We develop a method for 3D ultrasound spatial calibration based on intra-modality image registration that can be performed using any ultrasound imaging phantom, and characterize the method using a novel validation metric. We also present a new method for temporal synchronization of ultrasound images that leverages ultrasound probe motions generated by the robotic manipulator. Using calibrated 2D ultrasound images, we next describe a technique for monitoring real-time prostate displacements. Two image-based parameters were used to detect in-plane and out-of-plane displacements relative to an initial target position. With 95% confidence, the proposed method detected in-vivo prostate displacements before they exceeded 2.3, 2.5, and 2.8 mm in anteroposterior, superoinferior, and mediolateral directions. Given that typical prostate treatment margins are 5 mm, this technique showed significant promise for margin reduction and reduced healthy tissue toxicity relative to existing guidance techniques. In certain imaging positions, the robotic manipulator and ultrasound probe can interfere with radiation beams. To account for this, we developed a software environment that enables 3D visualization of image guidance hardware and radiation beams during treatment planning. With aid from the software, we re-planned treatments for prostate and liver cancer patients while avoiding interfering beam directions, and showed that clinically-deployed plans and plans with restricted sectors are similar. With reduced dose margins, as enabled by real-time imaging, gross tumor volume coverage was nearly identical while notable reductions of healthy tissue volumes exposed to large doses were possible. Our work demonstrates the feasibility of robotic ultrasound guidance for radiation therapy, and shows promise for the delivery of highly conformal cancer treatments that are truly adaptive to the continuous changes of internal anatomy.
  • 2012From: Springer
    Vipul R. Patel (editor).
    Pt. 1. Introduction to robotic surgery -- Pt. 2. Robotic surgery of the prostate -- Pt. 3. Kidney and adrenals -- Pt. 4. Bladder cancer -- Pt. 5. Pediatric urology -- Pt. 6. Robotic microsurgery -- Pt. 7. Allied health -- Pt. 8. Executive.
  • 2008From: Springer
    Hubert John, Peter Wiklund (eds.).
  • 2013From: Springer
    Hubert John, Peter Wiklund, editors.
    Part I: Kidneys -- Surgical Anatomy of Kidneys and Adrenals -- Robotic Kidney Surgery -- Partial Resection of the Kidney for Renal Cancer -- Robotic Nephroureterectomy -- Robotic Pyeloplasty -- Part II: Adrenals -- Robotic Adrenal Surgery -- Part III: Pelvis -- Surgical Anatomy of Pelvic Lymph Nodes -- Robotic-Assisted Pelvic Lymph Node Dissection -- Pelvic Lymphadenectomy for Localised Prostate Cancer and Robot-Assisted Radical Prostatectomy -- Part IV: Bladder -- Surgical Anatomy of the Bladder -- Robotic-Assisted Radical Cystectomy for Bladder Cancer in the Female -- Male Robot-Assisted Radical Cystectomy -- Robotic-Assisted Intracorporeal Urinary Diversion -- Technique of Extracorporeal Urinary Diversion -- Robot-Assisted Laparoscopic Ureteral Reimplantation -- Part V: Prostate -- Surgical Anatomy of the Prostate for Radical Prostatectomy -- Anatomical Aspects of the Neurovascular Bundle in Prostate Surgery -- Part VI: Benign Disease -- Robotic Adenomectomy -- Part VII: Radical Prostatectomy -- Trans- and Extraperitoneal Approach for Robotic-Assisted Radical Prostatectomy -- Radical Prostatectomy: Anterior Approach -- Robotic Prostatectomy: The Posterior Approach -- Bladder Neck Dissection During Robotic-Assisted Laparoscopic Radical Prostatectomy -- Techniques of Nerve Sparing in Robot-Assisted Radical Prostatectomy -- Antegrade Robot-Assisted Radical Prostatectomy: Factors Impacting Potency Preservation -- Allogenic Nerve Interposition During Non-Nerve-Sparing Robot-Assisted Radical Prostatectomy -- The Apical Dissection -- Posterior Reconstruction of the Rhabdosphincter -- The Urethrovesical Anastomosis -- Robotic-Assisted Laparoscopic Radical Salvage Prostatectomy -- Outcome Measures After Robot-Assisted Radical Prostatectomy -- Urinary Incontinence After Robotic-Assisted Laparoscopic Radical Prostatectomy -- Part VIII: Reconstructive Urology -- Paediatric Pelvic Exenteration -- Robot-Assisted Laparoscopy for Genital Organ Prolapse -- Robot-Assisted Laparoscopic Repair of Supratrigonal Vesicovaginal Fistulae with Peritoneal Flap Inlay -- Robotic Surgical Training: Imparting Necessary Skills to Future Urologic Surgeons.
  • 2017From: ClinicalKey
    [edited by] Bernard Lombard, Philippe Céruse ; with the contribution of Nicolas Andreff [and fifty-one others].
    Using the past to understand the present : a short history of digital guidance in ENT -- Principles and concepts of surgical navigation -- Surgical navigation in ENT -- Fundamentals of surgical robotics -- Surgical robots at work -- The da Vinci® system: technology and surgical analysis -- Transoral robotic surgery (with the da Vinci® system) -- Other ENT applications of the da Vinci® system -- Alternative solutions for transoral robotic surgery (TORS) -- Robot-assisted endo- and transnasal surgery -- Robot-based otological surgery -- Robot-assisted suturing and microsurgery -- Surgical simulation and training for ENT surgery -- Surgical robotics: safety, legal, ethical and economic aspects -- Concluding remarks.
  • Emel Demircan.
    Potential benefits from human motion understanding range from rehabilitation and physical therapy to ergonomics design, sports training, and computer animation. Robotics-based reconstruction and synthesis of human motion is a powerful tool to study human motion. Understanding human motion requires accurate modeling of the kinematics, dynamics, and control of the human musculoskeletal system to provide the bases for the analysis, characterization, and reconstruction of their movements. These issues have much in common with the problems found in the studies of articulated body systems in robotics research. Task-based methods used in robotics may be leveraged to provide novel musculoskeletal modeling methods and physiologically accurate performance predictions. However, reproducing and synthesizing the basis of human movements in common robotics frameworks bring the following compelling challenges: Scaling detailed musculoskeletal models to the human subject. Reconstruction and redundancy resolution of human motion in presence of constraints. Characterization of human postural behaviors and dynamic skills. The analysis, control, and reconstruction approaches developed in this thesis deal with these challenges. In motion analysis, methodologies are developed to characterize human postural behaviors and dynamic skills in a unified framework including task, posture, and additional constraints such as contact with the environment and physiological capacity. Information, which is gained from musculoskeletal models that are mapped into the motion of the human, is exploited in a task-oriented simulation and control framework. Task-driven human performance metrics, including the criteria for operational space acceleration characteristics and human muscular effort, are developed and analyzed for human skills. Using these metrics, optimization criteria are introduced that take into account human skeletal kinematics, muscle activation, physiology, and dynamics, and that correlate to the observed motion characteristics. In motion control, algorithms are developed to control human musculoskeletal systems in real-time. A marker space control structure is established for the reconstruction of human motion by direct tracking of marker trajectories. Dynamic consistency between marker space tasks, posture, and additional constraints is achieved by recursive projections into the null spaces of higher priority tasks. The human motion control hierarchy is established in marker space following the natural tree-like branching structure of the human musculoskeletal model. The marker space reconstruction methodology allows computing full human motion dynamics in real-time. In addition, an approach for resolving muscle redundancies is developed based on a new hybrid electromyography and conventional computed muscle control method. These methodologies are validated through three-dimensional dynamic simulations of musculoskeletal models scaled to the subjects. Extensive motion capture experiments are conducted on human subjects of various skill levels including a tai chi master, an elite college-level golfer, a novice golfer, and a professional American footballer for several dynamic movements. Using real-world experimental data, dynamic simulations are exemplarily created and analyzed for golf swings, throwing motions, and gait. The robotics-based reconstruction and synthesis approaches introduced in this thesis provide an important basis for understanding natural human motion. These tools are applicable to efficient robot control and human performance prediction. Another important application is the synthesis of novel motion patterns in the areas of robotics research, athletics, rehabilitation, physical therapy, and computer animation.
  • 2014From: Springer
    Keith Chae Kim, editor.
    Robotics in General Surgery provides a comprehensive review of the current applications of the robotic platform in all the general surgery subspecialties. Additionally, for each subspecialty it serves as a procedure-oriented instruction manual in terms of technical details of procedures, including fundamentals of robot positioning and trocar placement, step-by-step description of procedures, comprehensive discussions of advantages, limitations, indications, and relative contraindications of using the robotic approach. The text also discusses the challenges and steps to overcoming these challenges in transitioning from a minimally invasive to a robotic practice/surgeon. Lastly, this volume addresses emerging technology in robotics and the impact that the robotics platform will have on not only practice of surgery, but also in the education of surgeons at all levels. Written by experts in the field of robotic surgery, Robotics in General Surgery is a valuable resource for general surgeons of all levels including residents, fellows and surgeons already in practice.
  • 2011From: Springer
    edited by Ashok Kumar Hemal, Mani Menon.
    The History of Robotic Surgery -- Robotic Instrumentation and Operating Room Setup -- Port Placement in Robotic Urologic Surgery -- Achieving Efficiency in the Operating Room: Step by Step -- Laparoscopy vs. Robotics: Ergonomics -- Does It Matter? -- Anesthetic Considerations for Robotic Urologic Surgery -- The Development of a Robotic Urology Program in the UK -- Robotic Urologic Surgery: How to Make an Effective Robotic Program -- Witnessing the Transition of Open to Robotic Surgery -- Patient-Side Surgeons: The Unsung Heroes of Robotic Surgery -- Training in Robotic Urologic Surgery -- Animal Laboratory Training: Current Status and How Essential Is It? -- Training of Operating Room Technician and Nurses in Robotic Surgery -- Impact of Virtual Reality Simulators in Training of Robotic Surgery -- Training, Credentialing, and Hospital Privileging for Robotic Urological Surgery -- Research in Urologic Oncology in an Era of Minimally Invasive Surgery -- Databases and Data Management for Robotic Surgery -- The Role of Scientific Journals in Disseminating New Technology -- Predicting Robotic Utilization in Urologic Disease: An Epidemiology-Based Model -- Development of the Vattikuti Institute Prostatectomy: Historical Perspective and Technical Nuances -- Transferring Knowledge of Anatomical Dissection from the Laboratory to the Patient: An Australian Perspective -- Robot-Assisted Radical Prostatectomy: A Prostate Surgeon's Perspective -- Cautery-Free Technique of Robot-Assisted Radical Prostatectomy: Impact on Nerve Preservation and Long-Term Outcome on Recovery of Sexual Function -- Current Concepts in Cavernosal Neural Anatomy and Imaging and Their Implications for Nerve-Sparing Radical Prostatectomy -- Robot-Assisted Radical Prostatectomy for Large Glands and Median Lobe -- Extraperitoneal Robot-Assisted Radical Prostatectomy: Simulating the Gold Standard -- The Retrograde Extraperitoneal Approach: Robotic Retrograde Extraperitoneal Laparoscopic Prostatectomy (RRELP). Technical Modifications for Robotic Prostatectomy -- Robotic Radical Prostatectomy: Cancer Control and Implications of Margin Positivity -- Techniques to Improve Urinary Continence Following Robot-Assisted Radical Prostatectomy -- Penile Rehabilitation After Robotic Radical Prostatectomy: The Best Strategy -- Laparoscopy or Robotic Radical Prostatectomy: Pros and Cons -- Complications of Robotic Prostatectomy -- Robotic Urologic Surgery: Robotic-Assisted Adrenalectomy -- Robot-Assisted Laparoscopic Radical Nephrectomy and Nephroureterectomy -- Robot-Assisted Partial Nephrectomy -- Robotic Urologic Surgery: Robot-Assisted Partial Nephrectomy -- Robotic Donor Nephrectomy: Technique and Outcomes -- An European Perspective -- Robot-Assisted Laparoscopic Pyeloplasty -- Robotic Surgery for Urolithiasis -- Ureteral Reconstruction Utilizing Robotic-Assisted Techniques -- Robotic or Laparoscopic Renal Surgery: Pros and Cons -- Robot-Assisted Radical Cystectomy in Male: Technique of Spaces -- Robotic-Assisted Laparoscopic Anterior Pelvic Exenteration for Bladder Cancer in the Female -- Robotic-Assisted Laparoscopic Extended Pelvic Lymph Node Dissection for Bladder Cancer -- Robot-Assisted Intracorporeal Ileal Conduit -- Robotic Urinary Diversion: Technique, Current Status, and Outcomes -- Robotic Bladder Surgery Complications: Prevention and Management -- Robotic Surgery for Ureteral Anomalies in Children -- Robotic Surgery of the Kidney in Children -- Robotic Bladder Surgery in Children -- Robotic Surgery in Urogynecology -- Robotic Repair of Vesico-vaginal Fistula -- Robotic Surgery in Male Infertility (Robotic-Assisted Microsurgery) -- Treater to Target: Experiences of a Prostate Cancer Participant -- My Prostate Cancer -- Telementoring and Telesurgery in Urology -- Robotic Systems: Past, Present, and Future.
  • 2008From: ClinicalKey
    [edited by] Joseph A. Smith, Jr., Ashutosh Tewari.
    This new reference is the first of its kind devoted to the exploding area of robotic-assisted urologic surgery. It covers setting up robotics and instrumentation, as well as adapting laparoscopic equipment to this exciting new technology. It also guides you through a full range of robotic procedures including prostatectomy, which is experiencing significant success and patient satisfaction by using robotic technology, as well as nephrectomy, adrenalectomy, vasovasostomy, and pediatric procedures. Full color illustrations help familiarize you with the latest surgical techniques and instrumentation. Cuts patient recovery time in half with coverage of robotic assisted prostatectomy. Covers all urologic procedures that are adaptable to robotic technology with chapters on cystectomy, nephrectomy, prostatectomy, vasovasostomy, and adrenalectomy. Offers full color images of procedures to enhance surgical concepts.
  • Julia Oh.
    Accurate, large-scale gene annotation is a major challenge in biology. Traditionally, gene function can be assigned by observing the phenotype of a null mutant under various conditions. This principle has engendered the creation of genome-wide deletion collections in which each gene in a genome is knocked out or disrupted. Phenotype can then be assayed for each gene knockout individually. Parallelization of phenotypic assays via the introduction of molecular barcodes has proved invaluable for interrogating genome-wide collections of mutants in many conditions. This approach has been most visibly successful in the case of the budding yeast Saccharomyces cerevisiae, but it has the potential to bring insight to the genomes of many more microorganisms. The strategy of creating the genome-wide mutant collections that permit parallel phenotypic analysis has not been broadly applied beyond S. cerevisiae due primarily to technical limitations. In this dissertation, we describe a universal approach to rapidly generate comparable tagged, mutant collections. This approach combines DNA tag technology with transposon mutagenesis, and thereby can be generalized to any microorganism amenable to transposon mutagenesis. We created a universal collection of tags whose utility can extend to any application requiring sample tracking or multiplexing. We describe the validation of this tag resource as well as its use in the transposon mutagenesis of two different microorganisms, the bacteria Shewanella oneidensis MR-1 and the yeast Candida albicans. We then describe the expansion of this method to generate a genome-wide tagged transposon mutant collection in C. albicans. We used this collection to identify i) haploinsufficient genes in different nutrient conditions, and ii) mechanisms of drug-induced haploinsufficiency in C. albicans, and illustrated how these data can be used as a resource for genome annotation and hypothesis generation. Moreover, these studies illustrate why direct study of C. albicans is necessary, because relying solely on similarities between its traditional model organism, S. cerevisiae, would result in exclusion of C. albicans-specific genes and processes that are involved in its pathogenesis and may prove to be novel therapeutic targets.
  • 2014From: CRCnetBASE
    Rabindra Nath Das.
    "The present book initiates the concept of robust response surface designs, along with the relevant regression and positive data analysis techniques. Response surface methodology (RSM), well-known in literature, is widely used in every field of science and technology such as Biology, Natural (Physical/Chemical), Environmental, Medical, Agricultural, Quality engineering etc. RSM is the most popular experimental data generating, modeling and optimization technique in every field of science. It is a particular case of robust response surface methodology (RRSM). RSM has many limitations, and RRSM aims to overcome many of such limitations. Thus, RRSM will be much better than RSM. It is intended for anyone who knows basic concepts of experimental designs and regression analysis. This is the first unique book on RRSM. Every chapter is unique regarding its contents, presentation and organization. Problems on robust response surface designs such as rotatability, slope-rotatability, weak rotatability, optimality, and along with the method of estimation of model parameters, positive data analysis techniques are considered in this book. Some real examples on lifetime responses, resistivity, replicated measures, medical, demography, hydrogeology data etc., are analysed. Some examples (considered in this book) on design of experiments do not satisfy the classical assumptions of response surface methodology."-- Provided by publisher.
  • 2015From: Ovid
    editors: Charles M. Court-Brown, MD, DRCS, ED (Orth), Professor of Orthopaedic Trauma, Royal Informary of Edinburgh, Edinburgh, United Kingdom, James D. Heckman, MD, Editor-in-Chief, The Journal of bone & Joint Surgery, Needham, Massachusetts, Clinical Professor of Orthopaedic Surgery, Harvard Medical School, Visiting Orthopaedic Surgeon, Department of Orthopaedic Surgery, Massachusetts General Hospital, Boston, Massachusetts, Margaret M. McQueen, MD, FRCS, Ed (Orth), Professor of Orthopaedic Trauma, Royal Informary of Edinburgh, Edinburgh, United Kingdom, William M. Ricci, MD, Professor and Chief, Orthopaedic Trauma Service, Department of Orthopaedic Surgery, Washington University School of Medicine, St. Louis, Missouri, Paul Tornetta III, MD, Professor and Vice Chairman, Department of Orthopaedic Surgery, Boston University School of Medicine, Director of Orthopaedic Trauma, Boston Medical Center, Boston, Massachusetts, ; associate editor, Michael D. McKee, MD, FRCS(C), Professor, Upper Extremity Reconstructive Service, Department of Surgery, Division of Orthopaedics, St. Michael's Hospital and the University of Toronto, Toronto, Canada.
    "The 8th edition of Rockwood and Green's Fractures in Adults continues with the changes that were instituted in the 7th edition. In this edition there are two more chapters and 61 new authors drawn from three continents and eleven different countries... In addition, many of the new authors represent the next generation of orthopaedic trauma surgeons who will be determining the direction of trauma management over the next two or three decades"-- Provided by publisher.
  • 2006From: Ovid
    editors, Robert W. Bucholz, James D. Heckman, Charles M. Court-Brown ; associate editors, Kenneth J. Koval, Paul Tornetta III, Michael A. Wirth.
    Biomechanics of fixation and fractures / Allan F. Tencer -- Classification of fractures / Douglas R. Dirschl and Lisa K. Cannada-- Management of the multiply injured patient / Hans-Christoph Pape and Peter Giannoudis -- The epidemiology of fractures. pt. 1. Overview of epidemiology / Charles M. Court-Brown and Benjamin C. Caesar -- pt. 2. Experience in the United States / Kenneth J. Koval and Michael Cooley -- Nonoperative fracture treatment / John F. Connolly -- Principles of internal fixation / Christian Krettek and Thomas Gösling -- Principles of external fixation / J. Tracy Watson -- Bone and joint healing / Joseph A. Buckwalter, Thomas A. Einhorn and J.L. Marsh -- Bone grafting and enhancement of fracture repair / Sanjeev Kakar, Eleftherios Tsiridis, and Thomas A. Einhorn -- Outcome studies in trauma / Mohit Bhandari -- Imaging considerations in orthopaedic trauma / Andrew H. Schmidt and Kerry M. Kallas -- Initial management of open fractures / Steven A. Olson and Mark C. Willis -- Acute compartment syndrome / Margaret M. McQueen -- Penetrating trauma / Edward A. Perez -- War wounds, limb salvage, and traumatic amputations / Paul J. Dougherty -- Bone and soft tissue reconstruction / Lior Heller and L. Scott Levin -- Systemic complications / David C. Templeman and William A. Marinelli -- Local complications / Kirti D. Moholkar and Bruce H. Ziran -- Osteoporotic fractures / Magnus K. Karlsson, Karl Obrant and Per Olof Josefsson -- Pathologic fractures / Kristy L. Weber -- Stress fractures / David C. Teague -- Periprosthetic fractures. pt. 1. Periprosthetic fractures of the elbow and shoulder / Robert H. Cofield ... [et al.] -- pt. 2. Periprosthetic fractures about total hip and total knee replacements / Jay D. Mabrey -- Computer-aided orthopaedic surgery in skeletal trauma / Meir Liebergall, Rami Mosheiff, and Leo Joskowicz -- Fractures and dislocations of the hand / Mark H. Henry -- Fractures and dislocations of the carpus / Christian Gaebler -- Fractures of the distal radius and ulna / David S. Ruch -- Fractures of the shafts of the radius and ulna / Ralph Hertel and Dominique A. Rothenfluh -- Fractures and dislocations of the elbow / David Ring -- Fractures of the distal humerus / C. Michael Robinson -- Fractures of the shaft of the humerus / Michael D. McKee -- Fractures of the proximal humerus / Jon J. P. Warner, John G. Costouros and Christian Gerber -- Fractures of the clavicle / Mark D. Lazarus and Carl Seon -- Fractures of the scapula / Kenneth P. Butters -- Subluxations and dislocations about the glenohumeral joint / Young W. Kwon and Joseph D. Zuckerman -- Acromioclavicular joint injuries / Leesa M. Galatz and Gerald R. Williams, Jr. -- Injuries to the sternoclavicular joint / Gregory J. Gilot, Michael A. Wirth and Charles A. Rockwood, Jr. -- Principles of spine trauma care / Sohail K. Mirza, Carlo Bellabarba and Jens R. Chapman -- Injuries of the craniocervical junction / Carlo Bellabarba, Sohail D. Mirza and Jens R. Chapman -- Fractures and dislocations of the lower cervical spine / Christopher M. Bono -- Fractures and dislocations of the thoracolumbar spine / Robert K. Eastlack and Christopher M. Bono -- Fractures of the pelvic ring / Adam J. Starr and A. Steven Malekzadeh -- Fractures of the acetabulum / Mark C. Reilly -- Hip dislocations and fractures of the femoral head / Paul Tornetta III -- Fractures of the neck of the femur / Ross K. Leighton -- Intertrochanteric fractures / Kenneth J. Koval and Robert V. Cantu -- Subtrochanteric fractures / Kwok-sui Leung -- Fractures of the shaft of the femur / Sean E. Nork -- Fractures of the distal femur / Peter J. OBrien ... [et al.] -- Fractures of the patella and injuries to the extensor mechanism / Robert M. Harris -- Fractures of the proximal tibia / Kenneth A. Egol and Kenneth J. Koval -- Dislocations and fracture-dislocations of the knee / Robert C. Schenck, Jr., James P. Stannard, and Daniel C. Wascher -- Fractures of the tibia and fibula / Charles M. Court-Brown -- Ankle fractures / J.L. Marsh and Charles L. Saltzman -- Fractures of the talus / David W. Sanders -- Fractures of the calcaneus / Roy W. Sanders and Michael P. Clare -- Fractures and dislocations of the midfoot and forefoot / John S. Early.
    Also available: Print – v. 1-2.
  • 2010From: Ovid
    editors, Robert W. Bucholz, James D. Heckman, Charles M. Court-Brown, Paul Tornetta, III ; associate editors, Margaret M. McQueen, William M. Ricci.
    Biomechanics of fractures and fracture fixation -- Classification of fractures -- The epidemiology of fractures -- Bone and joint healing -- Biological and biophysical technologies for the enhancement of fracture repair -- Principles of nonoperative fracture treatment -- Principles of internal fixation -- Principles of external fixation -- Management of the multiply injured patient -- Initial management of open fractures -- Gunshot and wartime injuries -- Principles of mangled extremity management -- Amputations -- Bone and soft tissue reconstruction -- Outcome studies in trauma -- Imaging considerations in orthopaedic trauma -- Computer-aided orthopaedic surgery in skeletal trauma -- Principles of osteoporosis and fragility fractures -- Stress fractures -- Pathologic fractures -- Periprosthetic fractures -- Systemic complications -- Principles of complex regional pain syndrome -- Orthopaedic infections and osteomyelitis -- Principles of nonunion treatment -- Principles of malunions -- Acute compartment syndrome -- Hand fractures and dislocations -- Carpus fractures and dislocations -- Distal radius and ulna fractures -- Radial and ulnar shaft fractures -- Elbow fractures and dislocations -- Distal humerus fractures -- Humeral shaft fractures -- Proximal humerus fractures -- Clavicle fractures -- Scapular fractures -- Glenohumeral joint subluxations, dislocations, and instability -- Acromioclavicular joint injuries -- Sternoclavicular joint injuires -- Principles of spine trauma care -- Cervical spine fractures and dislocations -- Thoracolumbar spine fractures and dislocations -- Pelvic ring fractures -- Acetabulum fractures -- Hip dislocations and fractures of the femoral head -- Femoral neck fractures -- Intertrochanteric fractures -- Subtrochanteric fractures -- Femoral shaft fractures -- Distal femur fractures -- Patella fractures and extensor mechanism injuries -- Tibial plateau fractures -- Knee dislocations and fracture-dislocations -- Tibia and fibula fractures -- Pilon fractures -- Ankle fractures -- Talus fractures -- Calcaneus fractures -- Fractures and dislocations of the midfoot and forefoot.
    Also available: Print – v. 1-2, 2010.
  • 2017From: ClinicalKey
    [edited by] Charles A. Rockwood, Jr., Frederick A. Matsen, III, Michael A. Wirth, Steven B. Lippitt, Edward V. Fehringer, John W. Sperling ; associate editors, Samuel Antuña, Aaron J. Bois, Frank A. Cordasco, Jason E. Hsu.
    Developmental anatomy of the shoulder and anatomy of the glenohumeral joint -- Gross anatomy of the shoulder -- Clinical evaluation of shoulder problems -- Radiographic evaluation of shoulder problems -- Effectiveness evaluation of the shoulder -- Fractures of the proximal humerus -- Fractures of the scapula -- Fractures of the clavicle -- Disorders of the acromioclavicular joint -- Disorders of the sternoclavicular joint -- Sepsis of the shoulder : molecular mechanisms and pathogenesis -- Fractures, dislocations, and acquired problems of the shoulder in children -- Glenohumeral instability -- The rotator cuff -- Shoulder arthroscopy -- Glenohumeral arthritis and its management -- The biceps tendon -- Nerve problems related to the shoulder -- The stiff shoulder -- The shoulder in athletes -- Occupational shoulder disorders -- Tumors and related conditions.
  • 2015From: Ovid
    editors, John M. (Jack) Flynn, MD, Associate Professor of Orthopaedic Surgery, University of Pennsylvania School of Medicine, Associate Chief of Orthopaedic Surgery, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, David L. Skaggs, MD, Chief of Orthopaedic Surgery, Choildren's Hosptal Los Angeles, Professor of Orthopaedic Surgery, University of Southern California School of Medicine, Los Angeles, California, Peter M. Waters, MD, Clinical Chief of Orthopaedic Surgery, Boston Children's Hospital, Professor of Orthopaedic Surgery, John E. Hall Professor of Orthopaedic Surgery, Harvard Medical School, Boston, Massachusetts.
    "For the gold-standard resource on pediatric fractures, reach for Rockwood and Wilkins' Fractures in Children. Written by leading orthopaedic surgeons from around the world, the revised and expanded 8th edition of this classic bestselling text presents complete, up-to-date coverage of all types of children's fractures. A must-read for pediatric orthopedic surgeons and orthopedic residents. Features: NEW expanded coverage of pain medicine and casting; NEW 6 new authors from around the world; NEW time-saving outline template for easy quick-reference. Step-by-step instructions and expert guidance on numerous operative and management techniques. Author's Preferred Technique section includes algorithms explaining why the author chose that procedure. Pearls and Pitfalls and preventive measures listed for all procedures. NEW 13 full length procedural videos. Casting, remodeling and unique features of children's fractures. Now with the print edition, enjoy the bundled interactive eBook edition, offering tablet, smartphone, or online access to: Complete content with enhanced navigation, A powerful search that pulls results from content in the book, your notes, and even the web, Cross-linked pages, references, and more for easy navigation, Highlighting tool for easier reference of key content throughout the text, Ability to take and share notes with friends and colleagues, Quick reference tabbing to save your favorite content for future use, 13 full length procedural videos"--Provided by publisher.
  • 2006From: Ovid
    editors, James H. Beaty, James R. Kasser.
    Incidence of fractures in children / Kaye E. Wilkins and Alaric J. Aroojis -- The biologic aspects of children's fractures / Cory J. Xian and Bruce K. Foster -- Pain relief and related concerns in children's fractures / Joseph R. Furman -- Management of the multiply injured child / Robert M. Kay and Vernon T. Tolo -- Physeal injuries and growth disturbances / Karl E. Rathjen and John G. Birch -- Pathologic fractures associated with tumors and unique conditions of the musculoskeletal system / John P. Dormans and John M. Flynn -- Child abuse / Robert M. Campbell, Jr. and Timothy Schrader -- Fractures and dislocations of the hand and carpus in children / Scott H. Kozin and Peter M. Waters -- Fractures of the distal radius and ulna / Peter M. Waters and Alexander D. Mih -- Injuries to the shafts of the radius and ulna / Charles T. Mehlman and Eric J. Wall -- Fractures of the proximal radius and ulna / Robert E. Eilert and Mark A. Erickson -- Monteggia fracture-dislocation in children / José F. de la Garza -- The elbow region: general concepts in the pediatric patient / James H. Beaty and James R. Kasser -- Supracondylar fractures of the distal humerus / James R. Kasser and James H. Beaty -- The elbow: physeal fractures, apophyseal injuries of the distal humerus, osteonecrosis of the trochlea, and t-condylar fractures / James H. Beaty and James R. Kasser -- Dislocations of the elbow / Anthony A. Stans and Stephen D. Heinrich -- Proximal humerus, scapula, and clavicle / John F. Sarwark, Erik C. King, and Scott J. Luhmann -- Cervical spine injuries in children / William C. Warner, Jr. and Daniel J. Hedequist -- Thoracolumbar spine fractures / Peter O. Newton -- Fractures of the pelvis / Roger F. Widmann -- Fractures and traumatic dislocations of the hip in children / R. Dale Blasier and Laurie O. Hughes -- Femoral shaft fractures / James R. Kasser and James H. Beaty -- Extra-articular injuries of the knee / David L. Skaggs -- Intra-articular injuries of the knee / Mininder S. Kocher -- Fractures of the shaft of the tibia and fibula / Stephen D. Heinrich and James F. Mooney III -- Distal tibial and fibular fractures / R. Jay Cummings -- Fractures and dislocations of the foot / James G. Jarvis and Paul J. Moroz.
    Also available: Print – 2006
  • editors, James H. Beaty, James R. Kasser ; associate editors, David L. Skaggs, John M. Flynn, Peter M. Waters.
    PrintStatus: Not Checked OutLane Catalog Record
    Epidemiology of fractures in children -- The biologic aspects of children's fractures -- Pain relief and related concerns in children's fractures -- Management of the multiply injured child -- Physeal injuries and growth disturbances -- Pathologic fractures associated with tumors and unique conditions of the musculoskeletal system -- The orthopaedic recognition of child maltreatment -- Fractures and dislocations of the hand and carpus in children -- Fractures of the distal radius and ulna -- Injuries to the shafts of the radius and ulna -- Fractures of the proximal radius and ulna -- Monteggia fracture-dislocation in children -- The elbow region: general concepts in the pediatric patient -- Supracondylar fractures of the distal humerus -- The elbow: physeal fractures, apophyseal injuries of the distal humerus, osteonecrosis of the trochlea, and T-condylar fractures -- Dislocation of the elbow -- Proximal humerus, scapula, and clavicle -- Cervical spine injuries in children -- Thoracolumbar spine fractures -- Fractures of the pelvis -- Fractures and traumatic dislocations of the hip in children -- Femoral shaft fractures -- Extra-articular injuries of the knee -- Intra-articular injuries of the knee -- Fractures of the shaft of the tibia and fibula -- Distal tibial and fibular fractures -- Fractures and dislocations of the foot.
  • 2010From: Springer Protocols
    edited by Ulrich Dirnagl.
    From bedside to bench: How clinical reality should instruct stroke modeling / P. Patak and D.M. Hermann -- How to avoid bumping into the translational roadblock / M. Macleod -- Modeling focal cerebral ischemia in rodents: Introduction and overview / V. Prinz and M. Endres -- Focal cerebral ischemia in the mouse and rat using the intraluminal suture-filament model / L. Belayev, M. Endres and V. Prinz -- Focal ischemia models: Middle cerebral artery occlusion induced by electrocoagulation, occluding devices, and endothelin-1 / I.M. Macrae -- Rodent models of thromboembolic stroke / C. Orset ... [et al.] -- Photochemical and endothelin models of focal brain ischemia / O.W. Witte -- Housing in an enriched environment: A tool to study functional recovery after experimental stroke / K. Ruscher and T. Wieloch -- Modeling risk factors and confounding effects in stroke / B. McColl ... [et al.] -- Effect of anesthesia in stroke models / R.J. Traystman -- Noninvasive brain imaging in small animal stroke models: Mri and pet / A.M. Planas -- Non-invasive optical imaging in small animal models of stroke / A. Wunder and J. Klohs -- Behavioral testing in mouse models of stroke / M. Balkaya and M. Endres -- Behavioral testing in rodent models of stroke / G.A.S. Metz -- Histology and infarct volume determination / C. Sommer -- Ethics of modeling of cerebral ischemia in small animals / U. Lindauer -- Quality control and standard operating procedures / U. Dirnagl -- Statistics in experimental stroke research: From sample size calculation to data description and significance testing / U. Dirnagl -- Complexities, confounders, and challenges in experimental stroke research: A checklist for researchers and reviewers / U. Dirnagl.
  • 2015From: Springer
    Weihua Gong, editor.
    This book introduces transplantation in rodents as useful tools used in studying transplant immunobiology. Several solid organs (kidney, heart, liver) transplant models in rodents are described in this book. It can help surgical quality and save surgical time. The first part of the book provides a review of rodent transplant tolerance induction, the role of gender and body-weight in rodent transplantation, surgical instruments and organ preservation solutions. In the second part of the book, various organ-transplantation techniques in rodents are discussed in individual chapters. This book presents uniform surgical procedures in mouse and rats, which produce comparable data, efficiently enhancing the translational research from bench to non-human primates and beyond. It will be of great value to transplant researchers, research fellows and clinicians in many surgical specialties. Editor Weihua Gong is an Associate Professor at the Department of Surgery, the 2nd Affiliated Hospital of Zhejiang University, China.
  • editor, David G. Nichols ; section editors, Alice D. Ackerman ... [et al.].
    PrintStatus: Not Checked OutLane Catalog Record
  • [edited by Mawson, C. O. Sylvester].
  • Di Wu.
    First, we take a look at the function of a specific type of RNA editing mediated by ADAR (adenosine deaminase that acts on RNAs). We test our hypotheses on ADAR activity and target specificity through transcriptome analysis in the context of exogenous RNAi response pathway in C. elegans. By examining of small RNA and mRNA populations in adar mutants and additional RNAi defective mutants, we show that the activity and specificity of the ADAR enzyme likely precludes the detection of its targets through conventional methods of sequence capture and analysis. Based on this understanding, we develop novel computational approaches for analysis of small RNA and mRNA populations and identify a broader set of ADAR targets, leading to a deeper understanding of the ADAR pathway, the conserved functionalities of the ADAR enzyme, and its relationship with the exogenous RNAi pathway in C. elegans. Second, we examine the dynamics of the transcriptome upon and after exposure to extreme heat. We hypothesize that the recovery response after removal from heat exposure might involve alternations in transcription regulation that are more complex than a reversal of the heat-shock induced effects, and that one such form of long-term regulation might involve the endogenous RNAi pathway and regulation via synthesis of endogenous siRNAs. By examining the dynamics of the C. elegans transcriptome during time-course of heat exposure and recovery, we reach a deeper understanding of heat shock biology and explore the relationship between endogenous siRNAs and their sequence complementary mRNA targets in the context of environmentally induced perturbance.
  • Diana Ŕios-Cardona
    PrintStatus: Not Checked OutLane Catalog Record
  • Robin Deis Trujillo.
    MicroRNA (miRNA) genes produce three noncoding RNA products: the long primary transcript (pri-miRNA), the ~70 nucleotide pre-miRNA, and the ~22-nt mature miRNA. Only the mature miRNA is considered to be the functional species of a miRNA gene in recognizing cognate target mRNAs and modulating their expression. However, mature miRNAs are processed from the primary transcript through sequential endonucleolytic steps. As a result, the mature miRNA sequence is present in all three RNA products of a miRNA gene. It has thus been intrinsically difficult to determine the contribution of each miRNA gene product to target repression. In fact, direct functional roles for pri- and pre-miRNAs have never been ruled out. Here we show that pri- and pre-miRNAs may not be mere transitory intermediates of mature miRNA biogenesis. We found that ectopic expression of the C. elegans miRNA gene let-7 (cel-let-7) in human culture cells results in the production of truncated pre- and mature miRNAs that lack the first two 5' nucleotides, one of which is the first nucleotide of the miRNA seed region (nucleotide SD1). We found this nucleotide to be required for repression of target reporters by cel-let-7 in these cells, demonstrating that pri-let-7 may have a direct role in target repression. Further, we show that the nucleotide sequence and structure of both the pri-/pre-let-7 loop and stem regions play a key role in miRNA gene function in reporter assays. In vitro and in vivo analyses indicated the significance of these regions may be in the mediation of a physical interaction between pri-let-7 and target RNAs. These observations suggest that regulatory information encoded in the structured pri-miRNAs, but absent from mature miRNAs, could be directly interpreted for target recognition and repression through RNA:RNA interaction. Intriguingly, some mutations in the loop nucleotide sequence also restored processing of the 5' ends of C. elegans pre- and mature let-7 in culture cells, demonstrating that the pri-/pre-miRNA loop region can also regulate the precision of mature miRNA biogenesis. Importantly, in the presence of functional pre- and mature let-7, cel-let-7 activity in target repression consists of both SD1-independent and SD1-dependent components, implying potential contributions by both pri- and mature let-7. Finally, we interrogated the effects of pri-/pre-let-7 loop mutations on their ability to rescue a let-7 loss-of-function mutant phenotype in C. elegans. Our results indicate decreased significance of these parameters in the control of worm vulval development, although context-dependent differences in mature miRNA biogenesis between heterologous culture and live animals may partially explain this discrepancy. Taken together the work presented here reveals a novel layer of regulatory complexity encoded in long primary miRNAs that may have broad implications in understanding the mechanisms by which miRNA genes control target expression.
  • 2007From: NAP
  • Kristin A. Maczko.
    PrintStatus: Not Checked OutLane Catalog Record
  • 2015From: Springer
    Ram Roth, Elizabeth A.M. Frost, Cliford Gervirtz, editors, Carrie L.H. Atcheson, associate editor.
    Part I: Evolution and Development of Missions -- Medical Missions: A Short History from There to Here -- The Evolution of Surgical and Humanitarian Missions -- The Global Burden of Surgical Need -- Comparative Economics of Anesthesia Care and the Global Anesthesia Workforce -- Planning a Mission, Fundraising, and Building a Team: The Kybele Experience -- Organizing and Developing Universal Anesthetic Equipment -- Materials Management -- What do Patients and Communities Expect of a Medical Mission? -- Anesthesia in Resource-Poor Settings: The Mďecins sans Frontir̈es Experience -- Quality Care: Maintaining Safety Through Minimum Standards -- Legal and Ethical Issues in Global Health: A Trip through the Vagaries of Truth and Culture -- Part II: Specific Case Related Implementation -- Strategies for Patient Assessment and Scheduling -- Postoperative Follow-up and Quality Maintenance -- Women?s Rights and the Right to Pain Relief in Surgery and Labor -- Saving Sight In Developing Countries -- Cardiac Anaesthesthesia in Low Resource Settings -- Anesthesia Considerations for Facial Deformity Repair in Lesser Developed Countries -- Implementing a Regional Anesthesiology Service in a Low Resource Setting -- The Management of Pain in Less Developed Countries -- Living the Mission in Serbia and Other Less Affluent Worlds -- Anesthetic Management During the Lebanese Civil War -- Trauma, War, And Managing Vascular And Orthopedic Injuries -- Emergency Deployment in Disaster Areas -- Part III: Education at Home and Abroad -- Missions as a Part of Medical Education and the Residency Curriculum -- Global Health Programs in Academic Centers.- Closing the Professional Gap in Europe Through Education -- The Role of the Visiting Anesthesiologist in In-Country Education -- Letters (Lessons) from Around the World (Vignettes) -- The Future of Anesthesiology and Global Health in a Connected World.
  • 2014From: ScienceDirect
    edited by Nicholas Johnson.
    A short introduction to disease emergence / Nicholas Johnson -- Patterns of foot-and-mouth disease virus distribution in Africa / Miriam B. Casey [and eleven others] -- Paroviruses of carnivores / Andrew B. Allison and Colin R. Parrish -- Rabies / Conrad Freuling [and four others] -- Lassa fever / Elisabeth Fichet-Calvet -- Henipaviruses / Glenn A. Marsh and Lin-Fa Wang -- The role of birds in the spread of West Nile virus / Paul Gale and Nicholas Johnson -- Rift Valley fever virus / Janusz T. Paweska and Petrus Jansen van Vuren -- From simian to human immunodeficiency viruses (SIV to HIV) / Denis M. Tebit and Eric J. Arts -- Hantavirus emergence in rodents, insectivores and bats / Mathias Schlegel [and four others] -- Nipah virus / David T.S. Hayman and Nicholas Johnson -- Synthesis / Philip R. Wakeley, Sarah North and Nicholas Johnson.
  • 2005From: Springer
    D.E. Griffin (ed.).
    Mechanisms of apoptosis during reovirus infection / P. Clarke ... [et al.] -- Poliovirus, pathogenesis of poliomyelitis, and apoptosis / B. Blondel ... [et al.] -- Neuronal cell death in alphavirus encephalomyelitis / D. E. Griffin -- HSV-induced apoptosis in herpes encephalitis / L. Aurelian -- The role of apoptosis in defense against baculovirus infection in insects / R. J. Clem -- The role of host cell death in Salmonella infections / D. G. Guiney -- Role of macrophage apoptosis in the pathogenesis of Yersinia / Y. Zhang, J. B. Bliska -- Entamoeba histolytica activates host cell caspases during contact-dependent cell killing / D. R. Boettner, W. A. Petri -- Interactions between malaria and mosquitoes : the role of apoptosis in parasite establishment and vector response to infection / H. Hurd, V. Carter, A. Nacer -- Apoptosis and its modulation during infection with Toxoplasma gondii : molecular mechanisms and role in pathogenesis / C. G. K. Luder, U. Gross -- Modulation of the immune response in the nervous system by rabies virus / M. Lafon -- Apoptotic cells at the crossroads of tolerance and immunity / M. Skoberne ... [et al.].
    Also available: Print – 2005
  • Pei-Ling Chen.
    PrintStatus: Not Checked OutLane Catalog Record
  • 2016From: Springer
    Dirk Lange, Ben Chew, editors.
    1. Bacteria in the Genitourinary Tract -- 2. Overview of Urinary Tract Infections -- 3. Pathogenic Mechanisms of Uropathogens -- 4. Urosepsis- Pathogenesis and Treatment -- 5. Struvite Stone Formation by Ureolytic Biofilm Infections -- 6. The Management of Infection Stones -- 7. The Use of Bacteria to Treat Recurrent Calcium Oxalate Kidney Stone Disease -- 8. Role of Oxalobacter Formigenes Colonization in Calcium Oxalate Kidney Stone Disease -- 9. BCG for the Treatment of Non-Muscle Invasive Bladder Cancer.
  • Bryan Carl Petzold.
    The mechanics of our skin determine how our skin and specialized mechanically-sensitive neurons within it are stressed and strained when touched. Understanding the interplay between skin mechanics and touch sensitivity is paramount in uncovering the fundamental molecular mechanisms underlying touch sensation and in understanding and treating touch insensitivity due to diseases like peripheral neuropathy. Despite this importance, the interplay between skin mechanics and sensitivity is poorly understood. The organism Caenorhabiditis elegans is an excellent model system in which to test the hypothesis that skin mechanics affect touch sensitivity. C. elegans detects body touch with just six mechanically-sensitive neurons. These neurons are embedded in the outer shell (consisting of the cuticle, hypodermis and body wall muscles) of the body plan, which dominates the overall body mechanics. If skin mechanics are important in touch sensitivity in C. elegans, stiffening or softening the outer shell should alter the ability of the animal to detect applied mechanical stimuli. I demonstrate that a piezoresistive cantilever force clamp system and methods that alter C. elegans body mechanics can be combined to directly test the hypothesis that mechanics modulate touch sensitivity. In particular, I build on prior work demonstrating that genetic mutation of cuticle proteins alters body stiffness, and use optogenetic modulation of body wall muscle tone to demonstrate that the body wall muscles modulate C. elegans body mechanics. Combining piezoresistive cantilevers capable of applying minute forces with these techniques to alter the mechanics of the body allows us to directly quantify the affect of body stiffening or softening on force or indentation depth sensitivity in C. elegans. I find that even small shifts in body stiffness have a significant effect on force sensitivity, with body stiffening leading to a reduction in force sensitivity and vice versa. Further, I demonstrate that indentation depth sensitivity is less affected by changes in body mechanics, suggesting that the mechanically-sensitive neurons involved in body touch in C. elegans respond more directly to applied indentation than to applied force. These findings emphasize the importance of considering skin mechanics in understanding the sense of touch and insensitivity due to diseases like peripheral neuropathy.
  • 2013From: Springer
    Sanjay K. Srivastava, editor.
    Role of Capsaicin in Cancer Prevention -- Role of Death Receptors Belonging to the TNF Family in Capsaicin-Induced Apoptosis of Tumor Cells -- Role of Capsaicin in Prostate Cancer -- Capsaicin and the Urinary Bladder -- Inhibition of Pancreatitis and Carcinogenesis by Capsaicin -- Cytochrome P450-Dependent Modification of Capsaicinoids: Pharmacological Inactivation and Bioactivation Mechanisms -- The Cancer-Suppressing and -Promoting Actions of Capsaicin -- Oxidative Stress by Capsaicin in Cancer.
  • Bhagat, Budh Dev.
    PrintStatus: Not Checked OutLane Catalog Record
    v. 1. Hypertension.
  • Jennifer Cynthia Shieh.
    Disruptions in neuronal migration have been implicated in a variety of human mental disorders, including epilepsy, autism, and schizophrenia. Despite the critical role of migration during nervous system development, the basic physical and cytoskeletal mechanisms of coordinated neuronal movement have not yet been fully characterized. A migrating neuron moves with morphologically distinct steps: a single leading process extends ahead of a stationary cell soma, followed by the formation of a cytoplasmic dilation ahead of the nucleus, subsequent movement of the nucleus into the dilation, and retraction of the cell rear. The temporal and spatial regulation of adhesion is important for the proper progression of these steps. I investigated the role of endocytosis in regulating adhesion during neuronal migration. Using genetic and pharmacological methods to disrupt endocytosis either in vitro or in vivo leads to altered neuronal migration. Introducing dominant negative clathrin or dynamin into the developing cortex delays radial migration in vivo. Pharmacologically inhibiting clathrin or dynamin reduces the velocity of anterior subventricular zone (SVZa) neurons migrating in a three-dimensional matrix in vitro. Components of clathrin endocytic machinery are localized to the dilation region of a migrating neuron at points of matrix attachment. The absence of adhesion molecules at the cell rear led us to the hypothesis that endocytosis weakens adhesions in the dilation to allow the cell rear to move forward during migration. In support of this hypothesis, exposing SVZa explants to pharmacological inhibitors of either clathrin or dynamin prevents migration out of explants, and neurons that have migrated out have altered morphology and adhesion molecule distribution. Neurons exposed to a dynamin inhibitor tend to have "tails" of membrane at the rear, and these tails contain adhesion molecules. The presence of adhesion molecules at the rear of migrating neurons exposed to a dynamin inhibitor supports the idea that endocytosis plays a role in regulating adhesion disassembly. Endocytosis likely plays a critical role in general neuronal migration regardless of the specific neuronal subtype, migration mode, or substrate.
  • Christina D'Aura Swanson.
    Rheumatoid arthritis (RA) is an autoimmune synovitis affecting approximately 0.5% of the population worldwide. In addition to T cells, B cells and macrophages, cells such as synovial fibroblasts promote inflammation in the synovium by producing cytokines and chemokines. At the same time, neovascularization provides nutrients and oxygen to the growing pannus and increased osteoclastogenesis causes bone destruction. RA serum and synovial fluid contains increased concentrations of epidermal growth factor receptor (EGFR) ligands; and synovial fibroblasts and endothelial cells in RA synovium express EGFR. Thus, we hypothesized that a specific EGFR tyrosine kinase inhibitor, erlotinib, would ameliorate murine collagen-induced arthritis (CIA). Erlotinib is currently prescribed for the treatment of non-small cell lung cancer and pancreatic cancer. Here, we demonstrate that erlotinib reduces the severity of established murine CIA and investigated the mechanisms of this inhibition. Erlotinib reduced: 1) EGF-induced proliferation of fibroblasts and human umbilical vein endothelial cells (HUVEC), 2) EGF-induced production of IL-8 and vascular endothelial cell growth factor (VEGF) by fibroblasts, and 3) the development of osteoclasts from bone marrow cells. Together, these findings suggest that EGFR plays a central role in the pathogenesis of RA and that EGFR inhibition may provide benefit in the treatment of RA.
  • John Francis Garcia.
    PrintStatus: Not Checked OutLane Catalog Record
  • 2016From: Springer
    György Siklósi.
  • 2009From: Springer
    Piri Welcsh, editor.
  • Susan Elizabeth Vleck.
    Glycoprotein H (gH) plays an essential role in virus binding, entry and fusion of the Herpesviridae. Varicella-zoster virus (VZV) is an important human pathogen that causes varicella (chicken pox) and herpes zoster (shingles). VZV gH function has not be analyzed in depth. gH function was demonstrated to be important for VZV pathogenesis in skin xenografts in vivo by administration of anti-gH mAb 206, a conformation dependent neutralizing antibody. Antibody administration prevented infection in 42% of skin xenografts, and reduced virus replication and lesion formation in the remaining skin xenografts. Antibody binding to gH altered gH localization following endocytosis, preventing gH trafficking to the trans-Golgi network for virus secondary envelopment. Antibody binding to gH within the virus envelope resulted in internalization of virus particles, possibly for targeted degradation. Deletion of ORF 37, which encodes gH, demonstrated that gH was essential for VZV pathogenesis. Mutational analysis demonstrated that the N-terminus of the protein formed a structural epitope required for efficient VZV pathogenesis in vivo. Several neutralizing anti-gH antibodies target this epitope. A region of the C-terminus was required for VZV pathogenesis, and for efficient virus-induced cell-cell fusion. Predicted α-helices that might act as heptad repeats or fusion peptides were also required for gH function and VZV pathogenesis. Cysteine residues were important for gH maturation and transport, and possibly for correct expression of gH on the cell surface. Altogether, these studies demonstrate the importance of structural and functional domains for gH-dependent fusion and VZV pathogenesis.
  • Madhumitha Nandakumar.
    Lipids have long been associated with beneficial and detrimental effects on health and disease. The influence of lipids on the immune system is both complex and diverse, but the mechanisms through which fats modulate innate immune function remain to be clarified. Using the Caenorhabditis elegans-Pseudomonas aeruginosa host-pathogen system, we have attempted to characterize the importance of lipid metabolism to infection responses mediated through the innate immune system. We present evidence for the specific manipulation of lipid metabolism in response to bacterial infection in C. elegans. Infection with Pseudomonas results in altered expression of genes involved in the synthesis of long-chain fatty acids (LCFAs) as well as the breakdown of fats through mitochondrial and peroxisomal beta-oxidation. We demonstrate that two 18-carbon LCFAs, gamma-linolenic acid (GLA) and stearidonic acid (SDA) are required for basal innate immunity in-vivo. Deficiencies in GLA and SDA result in increased susceptibility to bacterial infection, which is associated with reduced basal expression of a number of immune-specific genes. GLA and SDA are required to maintain basal activity of the p38 MAP kinase pathway, which plays important roles in protecting metazoan animals from infection and oxidative stress. Genetic analysis also allowed us to identify a monounsaturated fat, vaccenic acid (VA) that negatively influences C. elegans immune function through its effect on the DAF-2 insulin-like pathway. Decreases in VA levels result in increased resistance to pathogen infection and an associated increase in the expression of antimicrobial peptide and other immune-related genes. Decreased VA levels were also associated with increased nuclear localization of DAF-16, a FOXO transcriptional regulator of the insulin-like immune pathway in C. elegans. Interestingly, we found that the bacterial pathogen Pseudomonas aeruginosa takes advantage of the detrimental effects of VA to compromise the C. elegans host and make it more susceptible to infection. A Pseudomonas mutant defective in the production of VA is attenuated and is compromised in its ability to suppress host immune gene expression. Together we have demonstrated distinct roles for individual fatty acids in innate immune function and pathogen virulence. These results may provide novel avenues for the use of lipids as immune modulators and help identify new targets for potential antimicrobial treatments.
  • Peggie Cheung.
    Epigenetics is the study of heritable changes in gene expression that occur independent of changes in the primary DNA sequence. Chromatin structure defines the state in which genetic information is organized in the cell. The organization of this structure greatly influences the abilities of genes to be activated or silenced. In eukaryotic cells, 146 base pairs of DNA is wrapped around the histone octamer (two H2A/H2B dimers and one H3/H4 tetramer) forming the nucleosomes, the basic unit of chromatin. The nucleosome cores are connected by linker DNA sequences to further package into higher-order chromatin structures. In addition to the core histones, each histone contains an unstructured N-terminal tail. The histone tails are the sites of most of the post-translational modifications (PTMs), such as acetylation, methylation and phosphorylation. These modifications regulate the structure and function of chromatin through two general mechanisms. In the first model, histone modifications may play a direct role in altering chromatin structure. For example, histone acetylation neutralizes the positive charge of lysine residues and thus, affecting the interactions of the histones with DNA, transcription factors and other nucleosomes. Secondly, histone modifications can indirectly affect chromatin functions by serving as a binding platform for modular proteins and complexes. For instance, the methylation of histone H3 at lysine 9 is recognized specifically by the chromatin organization modifier (Chromo) domain of heterochromatin protein 1 (HP1), which contributes to the induction and the propagation of heterochromatin structure. Ten years ago, Strahl and Allis proposed a general idea of "histone code" hypothesis, which states that histone modifications, distinct or in combination, to form a "code" to influence chromatin structure and lead to varied transcriptional outputs. In recent years, many chromatin regulators were identified, such as the proteins that "write" or "erase" or "read" the modifications. Some chromatin regulators are expressed in a tissue-specific manner and play important roles in physiology and disease pathogenesis. For instance, the H3K27 histone methyltransferase EZH2 is overexpressed in tumors such as prostate, breast, colon, skin and lung cancer. Disruption of normal patterns of covalent histone modifications is another hallmark of cancer. One of the most characterized examples is the global reduction of the trimethylation of H4K20 and acetylation of H4K16, along with hypomethylation, at repeat sequences in many tumors. Since post-translational modifications have been shown to be important for many biological processes such as gene expression, DNA damage and repair and apoptosis, disruption of these processes has been linked to carcinogenesis and other disease pathogenesis. The discovery of reversible mutations in the epigenetic machinery makes post-translational modifications as one of the most promising and expanding fields in the current biomedical research. Methylation does not neutralize the charge of the modified residue nor does addition of methyl groups add considerable bulk, this mark is believed to create a distinct molecular architecture on histones that is recognized by specialized binding domains present within chromatin-regulatory proteins. The proteins and domains that recognize histone modifications, named "effectors" or "readers", are thought to define the functional consequences of lysine methylation by transducing molecular events at chromatin into biological outcomes. Mutations in these "readers" proteins have been shown to link to many disease pathogenesis. However, relatively few effector domains have been identified in comparison to the number of modifications present on histones and non-histone proteins. Here we developed a human epigenome peptide microarray platform (HEMP) for high-throughput discovery of chromatin effectors. We probed this platform with modification-specific antibodies and known chromatin effector domains to test the integrity of the peptides on the slides. We also screened a library of Royal Domain family members and identified three effector proteins with novel modified-histone binding activity. Hence, the development of the HEMP facilitates the identification of effector proteins and understanding of chromatin signaling networks. Multiple Myeloma (MM) is a malignancy of bone marrow plasma cells that frequently results in bone marrow destruction, bone marrow failure and death. 15% of patients with multiple myeloma is diagnosed with an immunoglobin gene, t(4; 14), translocation. MM patients carrying the t(4; 14) translocation is associated with the overexpression of WHSC1/MMSET/NSD2. NSD2 is a protein lysine methylatransferase in the nuclear receptor binding SET domain protein family. However, the molecular mechanism by which NSD2 contributes to myeloma pathogenesis is not known. Here we show that the dimethylation of histone H3 at lysine 36 (H3K36) is the principal physiological activity of NSD2. In mammalian cells, H3K36me2 normally maps to gene bodies. In t(4; 14)+ myeloma cells, overexpression of NSD2 disrupts the physiologic genomic organization of H3K36me2 which is found being dispersed throughout the genome. NSD2 expression is linked to transcription activation and H3K36me2 location at gene bodies positively correlates with transcription levels. In Myeloma cells, NSD2-mediated localized elevation of H3K36me2 induces transcription at normally inert cancer-associated genes. Catalytic activity of NSD2 confers tumor formation in xenograft model and promotes oncogenic transformation of primary cells by regulating transcriptional programs that favor oncogenesis. The BAH domain is an evolutionarily conserved chromatin-associated motif. Utilizing the HEMP, we screened several BAH domains from yeast and human for binding activity. We found that the BAH domain of human ORC1 specifically bind to H4K20me2 peptides. Structural studies show that BAH domain has an aromatic dimethyl-lysine-binding cage that interacts with the bound peptide. ORC1 is dispensable for ORC complex assembly but is necessary for loading of the complex into chromatin. The ability of ORC1 BAH domain binding to H4K20me2 is required for the efficient stabilization of ORC complex at chromatin. H4K20me2 is enriched at replication origins. Abrogation in ORC1 and H4K20me2 interactions impairs cell-cycle progression. Mutations in ORC1 BAH domain have been implicated in aetiology of Meier-Gorlin syndrome (MGS), a form of primordial dwarfism. In a zebrafish model, orc1 morphants display an MGS-like dwarfism phenotype, which can be rescued by wild type Orc1 but not ORC1 binding mutants. Zebrafish depleted with H4K20me2 also displays the MGS-like phenotype. Together, our findings reveal a new function for histone methylation signaling at chromatin in the regulation of DNA replication and organismal growth. KDM2A is the first jumonjiC domain-containing demethylase identified. We solved the co-crystal structure of KDM2A and its substrate, H3K36me2. We found that KDM2A demethylation activity is required to maintain genomic stability. We also show that KDM2A is a tumor suppressor and its demethylation activity is required for suppressing cellular transformation.
  • Carl Benton Moree, III.
    Accurate chromosome segregation is essential for the viability of all organisms. During mitosis, each chromosome attaches to microtubules of the mitotic spindle through a chromosomal microtubule-binding site called the kinetochore. Kinetochores assemble on a specialized chromosomal locus termed the centromere, characterized by the replacement of histone H3 in centromeric nucleosomes with the essential histone H3 variant centromere protein A (CENP-A). Understanding how CENP-A chromatin is assembled and maintained is central to understanding chromosome segregation. The assembly of CENP-A into centromeric chromatin requires the activities of the Mis18 complex and the CENP-A chaperone HJURP, however how these proteins are recruited to centromeric chromatin is not known. In this work, we use an in vitro CENP-A assembly assay in Xenopus extract to examine the role of Mis18BP1, a component of the Mis18 complex, in CENP-A nucleosome assembly. We show that depletion of CENP-C prevents M18BP1 recruitment to centromeres and inhibits CENP-A chromatin assembly. We find that the recruitment of M18BP1 to centromeres occurs through a direct interaction with conserved domains in CENP-C. These findings suggest that the factors required for CENP-A assembly are recruited by the existing centromeric chromatin to ensure the assembly of new CENP-A nucleosomes at the site of the current centromere. In a separate set of experiments, we use an in vitro degradation assay in Xenopus extract to identify substrates of the Anaphase Promoting Complex or Cyclosome (APC/C) whose degradation regulates CENP-A assembly. We screen a collection of 20 centromere proteins and find that only M18BP1 is an APC/C substrate. We show that depletion of the APC/C from extract or mutation of the conserved destruction motifs prevented the degradation of M18BP1 in vitro. We also show that M18BP1 is degraded in vivo in a manner consistent with APC/C substrates. We find that the expression of a non-degradable M18BP1 mutant does not alter CENP-A assembly over the course of a single cell cycle, suggesting that there are multiple mechanisms in place to ensure the stable propagation of centromeric chromatin. In summary, we have identified a mechanism by which M18BP1 is recruited to centromeres to promote new CENP-A assembly. In addition, we have also demonstrated a novel role for the APC/C in regulating the activity of M18BP1. This work has furthered our understanding of how centromere chromatin is propagated and suggests a possible model in which CENP-C recruits M18BP1 to centromeres to promote new CENP-A assembly and that upon completion, M18BP1 is degraded by the APC/C to restrict CENP-A nucleosome assembly to a specific window in the cell cycle.
  • 2014From: Springer
    I.W. Fong.
    This new volume in the series Emerging Infectious Diseases of the 21st Century is a novel book on the role of microbes in the pathogenesis of common and disabling non-infectious diseases. New insights have emerged over the past several years suggesting that our commensal microflora of the gut is extremely important in regulating physiological and immune functions of the body. Covered are the perturbations of the normal composition of our endogenous microbiota, influenced by diet and genetic predispositions, as well as the mechanisms to produce common disorders such as obesity, diabetes, irritable bowel syndrome, colon cancer, and atherosclerotic vascular diseases. Also explored is the evidence suggesting that predisposition to increasingly common afflictions such as asthma and multiple sclerosis is influenced, in combination with our genetic composition, by early life exposure to environmental microbes and the time of onset of common viral infections. Chapters provide the most recent information on these disorders with regards to epidemiology, current concepts on pathogenesis and mechanisms of their biology, recent research and data on the role of microbes, analysis of their validity, and conclusive remarks and areas for future research. The Role of Microbes in Common Non-Infectious Diseases is an excellent resource for both physicians and investigators from a broad range of disciplines that will help to stimulate new concepts of disease pathogenesis, and lead to the unraveling of their mechanisms of diseases and to novel treatments. About the Author Ignatius [Bill] Fong is a Professor of Medicine at the University of Toronto and he is an author/ editor of 8 previously published books on Infectious Diseases. His research interests include topics in clinical infectious disease, HIV infection, and microbial-induced or -associated atherosclerosis.
  • Jessica Ann Ferreyra.
    Clostridium difficile is the most common cause of infectious antibiotic associated diarrhea, however the mechanisms underlying its ability to expand after antibiotics have not yet been described. Understanding how a dysbiotic gut microbiota is able to promote C. difficile, and conversely, how a healthy gut microbial community inhibits C. difficile are of great interest. The work in this thesis has led to an understanding of the instrumental roles that the microbiota plays in C. difficile gut colonization using gnotobiotic and conventional mouse models. Chapter 1 contains a review describing what is known about the important functions of the gut microbiota and its contribution to pathogenesis. In Chapter 2, we demonstrate that C. difficile and Salmonella typhimurium both utilize microbiota-liberated host sugars to expand upon antibiotic-treatment. In Chapter 3, we reveal other expansion strategies used by C. difficile and highlight the use of a microbiota-derived metabolite succinate by C. difficile to expand after perturbations. Chapter 4 contains three collaborative projects including two novel treatments for C. difficile-associated diseases, a prebiotic and a toxin inhibitor, as well as a new model used to study C. difficile-microbiota interactions. Finally Chapter 5 describes future considerations for this work and the field. These studies have revealed the basic mechanisms employed by C. difficile to expand upon antibiotic treatment and identified novel treatment strategies for C. difficile-associated diseases.
  • Lu-En Wai.
    Natural killer (NK) cells are an important component of the innate immune system. NK cells have cytotoxic and cytokine-producing abilities, and are known for their anti-tumor and anti-viral roles. On the other hand, the role of NK cells in solid organ transplantation is not clear. Our studies and others have shown that NK cells infiltrate allografts and NK cell depletion prevents graft rejection. We further described that NK cell depletion prevents tolerance induction in a rat liver transplant model of spontaneous tolerance. NK cell functions are determined by a balance of signals transmitted through their inhibitory and activating receptors. We showed that distinct NK activating receptors - NKp46, NKp30 and NKG2D, mediate NK cell interactions with dendritic cells (DC) and hepatoma tumor cells in vitro, presenting a way to perturb NK-DC interactions while retaining tumor responsiveness. Finally, initial experiments with NKG2D knockout mice showed that NKG2D deficiency combined with costimulation blockade prolongs allograft survival, suggesting that NKG2D inhibition may improve future costimulation blockade therapies. Importantly, current immunosuppressive drugs used to control graft rejection have differential effects on NK cell function. Thus understanding the interactions NK cells have with the graft and other immune cells in the context of transplantation is essential for improving transplantation therapeutics.
  • 2014From: CRCnetBASE
    editors, Isaias Dichi, José Wander Breganó, Andréa Name Colado Simão, Rubens Cecchini.
    Section 1. Introduction -- section 2. Oxidative stress and genetic polymorphism -- section 3. Oxidative stress in transmissible chronic diseases -- section 4. Oxidative stress in metabolic diseases -- section 5. Oxidative stress in autoimmune and neurodegenerative diseases -- section 6. Oxidative stress in cancer and cachexia.
  • Alper Yetil.
    MYC oncogene overexpression leads to rapid induction of tumors in animal models. We have previously shown that inactivation of MYC in tumors causes tumor regression. We identified senescence as a critical mechanism of MYC inactivation induced sustained tumor regression. This is the first time oncogene inactivation induced, in contrast to oncogene induced, senescence is reported. Here, we show that tumor suppressors p16INK4A, pRB and p19ARF are required for induction of senescence as well as sustained tumor regression. Either knockout of p53 or p19ARF accelerated MYC induced lymphomas and resulted in a dramatic increase in the incidence of tumor recurrence. Neither loss of p19ARF or p53 prevented proliferative arrest or apoptosis upon MYC inactivation. Loss of p19ARF, but not p53, was associated with a strong inhibition of senescence upon MYC inactivation. We conclude that p19ARF is essential for MYC inactivation to elicit sustained tumor regression through the induction of senescence. We also provide evidence that p19ARF mediates its effect on senescence in a p53 independent manner. We have uncovered that, in the absence of p19ARF, MYC inactivation in lymphomas leads to strong upregulation of NANOG, POU5F1, SOX2, GATA4, GATA6 and CCND1. Presence of either transgenic MYC or p19ARF represses the expression of these genes. Upregulation of self renewal associated genes NANOG, POU5F1 and SOX2 suggests that p19ARF null lymphomas may have expanded cancer stem cell fraction, which, in turn, could explain the propensity of p19ARF null lymphomas to reoccur. p16INK4A and p19ARF expression are regulated by BMI1. To study the role of BMI1 in cancer, senescence and stem cells, we generated a mouse model of conditional BMI1 overexpression. We present preliminary results in characterization of conditional BMI1 mouse. We compare tumors that are induced by MYC and by both MYC and BMI1. Mouse model of conditional BMI1 overexpression will be a useful tool in the study of cancer, senescence and stem cells.
  • 2014From: Springer
    Naranjan S. Dhalla, Sajal Chakraborti, editors.
    It is now well known that proteases are found everywhere, in viruses and bacteria as well as in all human, animal and plant cells, and play a role in a variety of biological functions ranging from digestion, fertilization, development to senescence and death. Under physiological conditions the ability of proteases is regulated by endogenous inhibitors. However, when the activity of proteases is not regulated appropriately, disease processes can result, as seen in Alzheimer's disease, cancer metastasis and tumor progression, inflammation and atherosclerosis. Thus it is evident that there is an absolute need for a tighter control of proteolytic activities in different cells and tissues. Aimed at graduate students and researchers with an interest in cellular proteolytic events, Role of Proteases in Cellular Dysfunctions is the second book on Proteases in this series. The book consists of three parts in specified topics based on current literatures for a better understanding for the readers with respect to their subject-wise interests. The first section of this book covers a brief idea about the neuronal disorders and the involvement of proteases such as calpains, caspases and matrix metalloproteases (MMPs). The second section covers the deadly disease cancer and its relation to ubiquitin-proteosomal system, MMPs and serine proteases. The last section is about the role of proteases such as calpains, MMPs and serine protease as well as urokinase type plasminogen activator receptor (uPAR) in causing cardiovascular defects.
  • Alya Rachel Raphael.
    Myelin is a specialized sheath that insulates axons and allows for the rapid conduction of action potentials. In the peripheral nervous system, myelin is made by glial cells called Schwann cells. Whereas Schwann cells have received attention mainly because of their role in generating myelin, they play many other important roles. Using the posterior lateral line nerve in zebrafish, I have investigated two aspects of Schwann cell development. First, I demonstrate a new role for Schwann cells in repositioning peripheral nerves. Second, I find that ErbB signaling and Schwann cell proliferation are required during radial sorting, a process during which Schwann cells change their interactions with axons and reorganize the structure of the nerve itself. The posterior lateral line nerve is a prominent peripheral nerve in zebrafish that innervates sensory organs called neuromasts. I find that the posterior lateral line nerve initially grows out within the epidermis but is then rapidly transitioned across the basement membrane into the subepidermal space. Schwann cells are required for this process; in three different mutants that lack Schwann cells, the nerve is consistently mislocalized to the epidermis. This mislocalization results in significant disorganization of the nerve that worsens over time. When wildtype Schwann cells are transplanted into mutants lacking Schwann cells the position of the nerve is rescued. These results provide evidence that moving the posterior lateral line nerve out of the epidermis, across the basement membrane, protects the nerve from the migration of its targets. Before Schwann cells can make myelin around an axon, they must go through several developmental steps. Immediately after Schwann cells and axons have completed their migration, each Schwann cell associates with many axons. In order for myelination to proceed, however, a Schwann cell must associate with only a single axon. This transition, termed radial sorting, results in significant reorganization of the nerve. Neuregulin signaling from axons through ErbB receptors on Schwann cells controls many aspects of Schwann cell development and in mammals both Neuregulin/ErbB signaling and Schwann cell proliferation have been implicated in radial sorting. This prompted me to investigate whether ErbB signaling was required to regulate Schwann cell proliferation during radial sorting. To test this I took advantage of the zebrafish model system and used small molecule inhibitors to inhibit either ErbB signaling or proliferation at the time when radial sorting is beginning. I find that ErbB signaling and Schwann cell proliferation are critical for radial sorting. ErbB signaling was also required for Schwann cells to extend processes into the axon bundle, while proliferation was not. Therefore, I propose that ErbB signaling is directly required during radial sorting to regulate Schwann cell process extension, in addition to the previously established role of ErbB signaling in stimulating Schwann cell proliferation.
  • Del Lucent.
    How a protein folds from an unstructured heteropolymer into a unique native structure is an important unanswered question in the field of molecular biophysics. In recent years, there have been a number of experiments and computer simulations that have provided insight into the mechanism by which folding occurs. Most of these experiments and simulations measure the dynamics of proteins in infinite dilution. However, bulk solvent is different from the cellular environment in which proteins truly fold. In vivo, protein dynamics occur in the context of the crowded cellular milieu as well as in confined spaces such as the chaperonin cavity, the proteosome, the ribosome exit tunnel, the translocon, etc. When considering these factors it is reasonable to assume that proteins may experience different microenvironments when folding in vivo than in bulk, and these differences may constitute a significant piece of the folding puzzle. Here we show via molecular dynamics simulation, that the presence of solvent when confining a mini- protein affects the probability and mechanism of folding. In a separate study, we use computer simulation to calculate the physical properties of water confined to the ribosome exit tunnel. We find that this solvent is quite different from bulk water, possessing a highly heterogeneous free energy landscape containing extensive water structure, areas of reduced solvent entropy. Finally we show that the foldase activity of GroEL cavity mutants is highly correlated with the hydrophilicy of the inner cavity.
  • 2013From: ScienceDirect
    edited by James S. Norris..
    Interdiction of sphingolipid metabolism to improve standard cancer therapies -- Diverse functions of ceramide in cancer cell death and proliferation -- Cermaide glycosylation catalyzed by glucosylceramide synthase and cancer drug resistance -- Use of acid sphingomyelinase for cancer therapy -- Hypoxia therapeuric resistance and sphingosine 1-phosphate -- Therapeutic potential of targeting SK1 in human cancers -- Sphinogosine kinase 1 in cancer -- Natural products as platforms for the design of sphingolipid-related anticancer agents.
  • Cecilia D. Sedano.
    Hepatitis C virus (HCV) is a small, hepatotropic, RNA virus that can establish persistent infections leading to chronic hepatitis and hepatocellular carcinoma (HCC). With an estimated 2% of the world's population infected, HCV is a serious global health problem. Curiously, this virus has the unique feature of depending on the liver-specific, microRNA miR-122. In the current model, miR-122 interacts with the 5'-end of the HCV RNA genome, protecting it from hitherto unknown degradation machinery. The work described in this dissertation highlights a novel cytoplasmic antiviral function for the mammalian 5'-3' exoribonuclease Xrn2, and enzyme best known for its role in RNA polymerase II transcription termination. We discovered that Xrn2 modulates HCV RNA abundance by destabilizing the viral RNA genome. Importantly, during sequestration of miR-122, Xrn2 depletion restored HCV RNA abundance, suggesting that Xrn2 depletion eliminates the miR-122 requirement for viral RNA stability. Thus, Xrn2 is an antiviral effector so potent that HCV has evolved a mechanism to evade it, through the subversion of miR-122 to form a protective oligomeric complex at the 5' end of the viral genome. To determine if Xrn2's antiviral function is specific to HCV, we studied its role in poliovirus (PV) infection. Our experiments showed that Xrn2 interacts with PV RNA early during infection, and that Xrn2 activity may be suppressed by proteolytic cleavage that is mediated by the PV protease 2A. This work revealed an unprecedented role for Xrn2 and highlighted the broad function of this novel antiviral factor in RNA virus infection. In this dissertation, we also investigated the regulation of noncoding RNAs in HCV infection. Sucrose-gradient analysis of miR-122 during HCV infection uncovered that this highly conserved miRNA and its isomiR variants form unique complexes, which are specifically modulated during infection. This highlights the ability of HCV to modulate the engagement of specific miR-122-complexes, and the distinct functions in gene regulation these complexes may play. We also examined the role of Xrn2 in mammalian long noncoding RNA (lncRNA) degradation, and their regulation during HCV infection, by performing lncRNA microarrays. Our analyses will provide important insights into the biological functions of lncRNAs and help us identify novel therapeutic targets for HCV infection and HCC. Taken together, the work described here has uncovered a novel cytoplasmic antiviral function for Xrn2 in RNA virus infection. Our research highlights the potential of studying lncRNAs and miRNA variants during viral infection, which can lead the way for the discovery of new biomarkers and therapeutics.
  • Darrell G. McPherson.
  • Eric Andrew Evans.
    PrintStatus: Not Checked OutLane Catalog Record
  • Kelsey Lynne Clark.
    Spatial attention is known to gate entry into short-term memory, and some evidence suggests that spatial signals may also play a role in binding features or protecting object representations during memory maintenance. To examine a potential role for spatial signals in maintaining object short-term memory, the activity of neurons in the Frontal Eye Field (FEF) of macaque monkeys was recorded during an object-based delayed match-to-sample (DMS) task. In this task monkeys were trained to remember an object identity over a brief delay, irrespective of the locations of the sample or target presentation. FEF neurons exhibited visual, delay, and target period activity, including selectivity for sample location and target location. Delay period activity represented the sample location throughout the delay, despite the irrelevance of spatial information for successful task completion. Furthermore, neurons continued to encode sample position in a variant of the task in which the matching stimulus never appeared in their response field. FEF neurons also exhibited target-position-dependent anticipatory activity immediately prior to target onset, suggesting that the monkeys can predict target position within blocks. These results show that FEF neurons maintain spatial information during short-term memory, even when that information is irrelevant for task performance. Despite the robust delay period activity we observed in FEF during the DMS task, we found little further evidence to support the theory that this activity contributes to object memory maintenance. Noise correlations were present between pairs of simultaneously recorded FEF and IT neurons during the sample and early delay periods, but did not persist into the second half of the delay period, despite the continued elevation of firing rates in both regions throughout the delay. The most direct method of assessing the contribution of the FEF delay period activity observed during the DMS task to object memory was the pharmacological elimination of that activity and evaluation of the impact on task performance. Inactivation of FEF with muscimol produced spatially localized deficits on the memory guided saccade task, but did not selectively impair object memory performance for sample stimuli appearing in the mnemonic scotoma.
  • Teresa F. Reyes.
    The lymphatic vasculature (LV) is an essential component of the cardiovascular system and fulfills critical roles during development and in the pathogenesis of diseases such as lymphedema, cancer metastasis and various inflammatory conditions. Although, there has been progress in the field of lymphatic vascular research, our understanding of lymphatic vessel development and pathogenesis is still rather limited. We have previously shown that the endothelial-specific microRNA-126 plays a crucial role in mediating developmental angiogenesis in vivo. In this study we demonstrate that miR-126 is also necessary for the formation of de novo lymphangiogenesis. To this extent, we have employed an in vivo approach by utilizing our miR-126 knockout (KO) mice as well as lymphatic specific driver mice (Prox1CreERT2) to address the role of miR-126 in the lymphatic vasculature. Regulation of lymphangiogenesis by miR-126 was confirmed using sophisticated in vivo models such as the tail lymphedema, corneal micropocket assay and intradermal injections with adenovirus encoding VEGF-C (VEGF-C challenge). In vitro studies have shown that miR-126 is highly expressed in both human and mouse lymphatic endothelial cells (LECs). MiR-126 knockdown in HLECs results in reduced proliferation and decreased cell viability with abrupt VEGF-C removal. Furthermore, VEGF-C induces miR-126 expression in a dose-dependent manner, suggesting that there is a molecular link between miR-126 and the VEGFR-3/VEGF-C signaling pathway. Our studies show that miR-126 can play a dual role not only in regulating angiogenesis but also lymphangiogenesis in vivo. Understanding the role of miR-126 in lymphangiogenesis provides further insight for the therapeutic manipulation of lymphatic vessels. Overall, this thesis has unveiled a novel role for miR-126 in lymphangiogenesis. Future studies are needed to elucidate the precise mechanism of action of miR-126 in this context are needed and are currently ongoing. It is our hope that gaining a deep understanding of how miR-126 regulates lymphatic endothelial cell physiology will provide valuable contributions towards improving our ability to treat cancer metastasis through manipulation of its principal dissemination avenue, the lymphatic vasculature.
  • 2007From: CRCnetBASE
    edited by Robert M. Bradley.
  • Oliver Crespo.
    Multiple Sclerosis (MS) is an autoimmune inflammatory disease of the central nervous system characterized by demyelination and variable axonal loss. Tyrosine kinases play a central role in the activation of signal transduction pathways and cellular responses that mediate the pathogenesis of MS. Imatinib mesylate (imatinib), sorafenib, and GW2580 are tyrosine kinase inhibitors (TKIs) developed to treat diverse types of cancers. This thesis demonstrates that Imatnib and Sorafenib, two PDGFR inhibitors and GW2580, a specific Fms inhibitor, can prevent and treat MOG-induced experimental autoimmune encephalomyelitis (EAE). My observations suggest that the c-Fms and PDGFR tyrosine kinase signaling pathways play a central role in the pathogenesis of EAE and MS. I believe that c-Fms primes macrophages to produce TNF and that PDGFR induces astrocytes to proliferate, which are cellular responses contributing to autoimmune demyelination in multiple sclerosis. Chapter 1 provides a general introduction to MS and EAE and the role different cell types of the immune system play in disease pathogenesis, paying particular attention to the role of macrophages and astrocytes. Chapter 2 describes in further detail the different functions mediated by MCSF in macrophages and how these are tied to the pathology of MS. In this chapter, the experiments performed to determine whether c-Fms inhibition using tyrosine kinase inhibitors could have an effect on pathogenic macrophage functions are described. Chapter 3 focuses on the immune functions of astrocytes, emphasizing those believed to be mediated by PDGFR signaling, and the experiments performed to test the effects of tyrosine kinase inhibitors on PDGFR-mediated astrocyte functions. Chapter 4 concludes with a discussion of the results and future directions for the use of tyrosine kinase inhibitors for the screening of potential targets involved in the pathogenesis of autoimmune diseases.
  • 2013From: Springer
    Jose Russo, Irma H. Russo.
    This book is designed for advanced students and researchers in cell biology, biochemistry, molecular biology, medicine in general, and cancer in particular. It provides the latest data on the transcriptome of the mammary gland in order to establish the molecular and cellular biology of differentiation leading to cancer prevention. The authors have based their work on the epidemiological evidence that early first full term pregnancy is a protective factor in humans against breast cancer and using this knowledge have developed in vivo and in vitro experimental systems that have demonstrated mechanistically how the differentiation takes place. The transcriptoma analysis of the female breast shows that an early first full term pregnancy reprograms the organ by imprinting a genomic signature that differs according to reproductive history. This reprogramming takes place at the chromatin level by changing the transcriptional process. The modification of the transcriptional control is due to the expression of non-coding RNA sequences and post-transcriptional control driven by the spliceosome. The plasticity of the genome of the human breast makes possible this reprogramming that is not only induced by the physiological process of pregnancy but by the use of hormones mimicking pregnancy. The role of stem cells and their reprogramming during differentiation are presented as a new paradigm in breast cancer prevention.
  • 2008From: Springer
    C. Schmidt ... [et al.].
  • John Kenneth Mich.
    The zebrafish has emerged as a powerful model organism for the experimental interrogation of the processes that bring about vertebrate development. In this thesis we study functions of Hedgehog (Hh) signaling, a process that is frequently dysregulated in cancer, that occur during the formation of the zebrafish embryo. First, we investigate a possible link between Hh signaling and the directed migration of germ cells to the presumptive gonad in the early embryo and find that Hh-targeting drugs can perturb germ cell migration, but Hh signaling itself does not regulate this process. Second, we study the formation of motoneurons in the developing ventral spinal cord and find that Hh signaling and retinoic acid signaling work together to induce these cells by the same downstream output of Gli transcription factor activity. Third, we generated transgenic zebrafish that reliably report Hh signaling events at multiple levels of the pathway and we apply these tools in initial studies of zebrafish regeneration. In summary, these studies further our knowledge of the roles for Hh signaling in vertebrate development and will lead insight into the pathology of Hh-related disorders.
  • Jonathan Isaiah Gent.
    PrintStatus: Not Checked OutLane Catalog Record
  • Matthew Frederick Barber.
    Sirtuins are a conserved family of NAD+-dependent deacetylases that regulate diverse biological processes including genomic stability, metabolism, and aging. SIRT7 is a mammalian sirtuin whose enzymatic activity and physiologic functions have been unclear. Here I present work aimed at elucidating the biochemical, molecular, and cellular roles of human SIRT7. In Chapter 1, I report the discovery that SIRT7 possesses deacetylase activity specific for histone H3 acetylated at lysine 18 (H3K18Ac). In genome-wide binding studies, we find that SIRT7 associates with promoters of a specific set of gene targets, where it deacetylates H3K18Ac and promotes transcriptional repression. In Chapter 2, I discuss the identification of a novel SIRT7 interacting protein, the ETS transcription factor ELK4. Our work suggests that ELK4 plays an important role in defining the spectrum of SIRT7 target genes, by recruiting SIRT7 to a subset of promoters. In Chapter 3, I present our work establishing new functions for SIRT7 in cancer progression. Notably, selective hypoacetylation of H3K18Ac has recently been linked to oncogenic transformation, and in patients is associated with aggressive tumor phenotypes and poor prognosis. We find that deacetylation of H3K18Ac by SIRT7 is necessary for maintaining essential features of human cancer cells including anchorage independent growth and escape from contact inhibition. Moreover, SIRT7 is necessary for a global hypoacetylation of H3K18Ac associated with cellular transformation by viral oncoproteins. Finally, SIRT7 depletion markedly reduces the tumorigenicity of human cancer cell xenografts in mice. Together, this work establishes SIRT7 as the first known site-specific H3K18Ac deacetylase and demonstrates a pivotal role for SIRT7 in chromatin regulation, cellular transformation programs, and tumor formation in vivo.
  • Kelly Elizabeth McCann.
    Efficient DNA double-strand break (DSB) repair is essential for maintaining the stability of the genome. A single long-lived DSB can cause cell lethality (Bennett et al., 1996). Humans with defects in DSB repair are sensitive to DNA damaging agents and are predisposed to developing cancers, as exemplified by such diseases as Nijmegan breakage syndrome, Ataxia telangiectasia, and breast cancer susceptibility in women with mutations in the BRCA1 and BRCA2 genes (Duker, 2002). Repair of DNA damage requires activation of cell cycle checkpoint controls, recruitment of repair proteins to DNA lesions, and transcriptional activation of relevant genes. As shown by our data, deletion of particular histone modification genes produces sensitivity to ionizing radiation in Saccharomyces cerevisiae, suggesting a role for chromatin modification enzymes in the repair process as well. Because damage recognition and repair of lesions are both influenced by chromatin structure, we began by studying the role of histone acetylation in the DNA damage response. Acetylation of the N-terminal tails of histone H4 opens the chromatin to allow repair enzymes to access broken DNA. Through a screen of the yeast deletion pool, we found that deletion of BRE1 and DOT1 genes causes sensitivity to ionizing radiation. Ubiquitination of H2B on lysine 123 (H2B-K123) by ubiquitin ligase Bre1 is necessary for methylation of H3 on lysine 79 (H3-K79) by Dot1. Through the histone modifications they catalyze, these proteins are involved in many aspects of the DNA repair response, as outlined in Sections C, D, and E. Our studies focused on homologous recombination repair defects, genome-wide expression patterns in BRE1 and DOT1 deletion mutants, an analysis of the data regarding proteins purported to bind to methylated H3-K79, and optimization of a protein purification strategy to find Dot1 binding partners. Our yeast deletion pool screen also predicted a role for N-terminal acetyltransferase complex NatB in DNA double-strand break repair. In Section F, we build a case for acetylation of DNA end-binding protein Mre11.
  • Roberto Rafael Ricardo-Gonzalez.
    PrintStatus: Not Checked OutLane Catalog Record
  • 2009From: Springer
    Jürgen Tautz ; con fotografie di Helga R. Heilmann ; [traduzione di Massimo Caregnato].
  • 2011From: Springer
    Antonio Costa de Oliveira, Rajeev K. Varshney, editors.
    With the predicted increase of the human population and the subsequent need for larger food supplies, root health in crop plants could play a major role in providing sustainable highly productive crops that can cope with global climate changes. While the essentiality of roots and their relation to plant performance is broadly recognized, less is known about their role in plant growth and development. "Root Genomics" examines how various new genomic technologies are rapidly being applied to the study of roots, including high-throughput sequencing and genotyping, Tilling, transcription.
  • 2014From: OSO
    edited by Anjan Chatterjee and H. Branch Coslett.
    Ch. 1. The Case for Case Reports / Kenneth M. Heilman -- Ch. 2. We Stand on the Shoulders of Giants: The Golden Era of Behavioral Neurology 1860 - 1950 and Its Relevance to Cognitive Neuroscience Today / Heidi Roth -- Ch. 3. Deconstructing Human Memory: Insights from Amnesia / Margaret M. Keane -- Ch. 4. Semantic Memory / Leslie J. Gonzalez Rothi -- Ch. 5. Alexias and Agraphias / Elizabeth H. Lacey -- Ch. 6. Face Recognition / Steven Z. Rapcsak -- Ch. 7. Arousal, Attention, and Perception / Mark Mennemeier -- Ch. 8. Perceptual-Attentional "Where" and Motor-Intentional "Aiming" Spatial Systems / A.M. Barrett -- Ch. 9. Limb Apraxia: A Disorder of Goal-Directed Actions / Anne L. Foundas -- Ch. 10. Body Representations: Updating a Classic Concept / H. Branch Coslett -- Ch. 11. The Neuropathologies of the Self / Todd E. Feinberg -- Ch. 12. The Neurology of Emotional Expression / Leex Blonder -- Ch. 13. Behavioral and Cognitive Effects of Antiepileptic Drugs / Kimford J. Meador -- Ch. 14. Neuropsychopharmacology and Cognition / David A. Beversdorf -- Ch. 15. Attractor Basins: A Neural Basis for the Conformation of Knowledge / Stephen E. Nadeau -- Ch. 16. Plasticity / Victor W. Mark -- Ch. 17. Visual Art / Anjan Chatterjee -- Ch. 18. Creativity / Glen R. Finney.
  • v.1-2=, 2011From: ClinicalKey
    Juan Rosai.
    V. 1 -- Introduction -- Gross techniques in surgical pathology -- Special techniques in surgical pathology -- Skin -- dermatoses -- Skin -- tumors and tumorlike conditions -- Oral cavity and oropharynx -- Mandible and maxilla -- Respiratory tract -- nasal cavity, paranasal sinuses, and nasopharynx -- Respiratory tract -- larynx and trachea -- Respiratory tract -- lung and pleura -- Mediastinum -- Thyroid gland (with giovanni tallini) -- Parathyroid glands -- Gastrointestinal tract -- esophagus -- Gastrointestinal tract -- stomach -- Gastrointestinal tract -- small bowel -- Gastrointestinal tract -- appendix -- Gastrointestinal tract -- large bowel -- Gastrointestional tract -- anus -- Major and minor salivary glands -- Liver -- non-neoplastic diseases -- Liver -- tumors and tumorlike conditions -- Gallbladder and extrahepatic bile ducts -- Pancreas and ampullary region -- Adrenal gland and other paraganglia -- Urinary tract -- kidney, renal pelvis, and ureter: non-neoplastic diseases -- Urinary tract -- kidney, renal pelvis, and ureter: pediatric tumors and tumorlike conditions/adult tumors and tumorlike conditions -- Urinary tract -- bladder -- Male reproductive system -- prostate and seminal vesicles -- Male reproductive system -- testis -- Male reproductive system -- testicular adnexa -- Male reproductive system -- penis and scrotum -- V. 2 -- Female reproductive system -- vulva -- Female reproductive system -- vagina -- Female reproductive system -- uterus: cervix -- Female reproductive system -- uterus: corpus -- Female reproductive system -- fallopian tube -- Female reproductive system -- ovary -- Female reproductive system -- placenta -- Breast -- Lymph nodes -- Spleen -- Bone marrow -- Bone and joints -- Soft tissues -- Peritoneum, retroperitoneum, and related structures -- Cardiovascular system -- heart -- Cardiovascular system -- arteries -- Cardiovascular system -- veins -- Cardiovascular system -- lymph vessels -- Central nervous system -- Pituitary gland -- Eye and ocular adnexa -- Ear.
  • 2007From: Ovid
    [edited by] Jeffrey Schaider, Stephen R. Hayden, Richard Wolfe, Roger M. Barkin, Peter Rosen.
    Also available: Print – 2007
  • 2011From: Ovid
    [edited by] Jeffrey J. Schaider ... [et al.].
    "This best-selling emergency department reference is now in its thoroughly updated Fourth Edition. The foremost authorities provide practical information on over 600 clinical problems in a fast-access two-page outline format that's perfect for on-the-spot consultation during care in the emergency department. Coverage of each disorder includes clinical presentation, pre-hospital, diagnosis, treatment, disposition, and ICD-10 coding. Icons enable practitioners to quickly spot the information they need. This edition provides up-to-date information on topics such as emerging infections, new protocols, and new treatments"--Provided by publisher.
    Also available: Print – 2011
  • 2015From: Ovid
    Jeffrey J. Schaider, MD, Roger M. Barkin, MD, MPH, Stephen R. Hayden, MD, Richard E. Wolfe, MD, Adam Z. Barkin, MD, MPH, Philip Shayne, MD, Peter Rosen, MD.
    "This best-selling emergency department reference is now in its thoroughly updated Fifth Edition. The foremost authorities provide practical information on over 600 clinical problems in a fast-access two-page outline format that's perfect for on-the-spot consultation during care in the emergency department.Coverage of each disorder includes clinical presentation, pre-hospital, diagnosis, treatment, disposition, and ICD-9 coding. Icons enable practitioners to quickly spot the information they need. This edition provides up-to-date information on topics such as emerging infections, new protocols, and new treatments"--Provided by publisher.
  • 2014From: Ovid
    Syed A. Hoda, Edi Brogi, Frederick C. Koerner, Paul P. Rosen.
    Anatomy and physiologic morphology -- Abnormalities of mammary development and growth -- Inflammatory and reactive tumors -- Specific infections -- Papilloma and related benign lesions -- Myoepithelial neoplasms -- Adenosis and microglandular adenosis -- Fibroepithelial neoplasms -- Ductal hyperplasia: usual and atypical -- Precarcinomatous breast disease: epidemiologic, pathologic, and clinical considerations -- Ductal carcinoma In Situ -- Invasive ductal carcinoma: assessment of prognosis with morphologic and biologic markers -- Tubular carcinoma -- Papillary carcinoma -- Medullary carcinoma -- Carcinoma with metaplasia and low-grade adenosquamous carcinoma -- Squamous carcinoma -- Mucinous carcinoma -- Apocrine carcinoma -- Mammary carcinomas with endocrine features -- Small cell carcinoma -- Secretory carcinoma -- Mammary carcinoma with osteoclast-like giant cells -- Cystic hypersecretory carcinoma and cystic hypersecretory hyperplasia -- Adenoid cystic carcinoma -- Invasive cribriform carcinoma -- Lipid-rich carcinoma -- Glycogen-rich carcinoma -- Invasive micropapillary carcinoma -- Paget disease of the nipple -- Lobular carcinoma In Situ and atypical lobular hyperplasia -- Invasive lobular carcinoma -- Unusual clinical presentation of carcinoma -- Metastases in the breast from nonmammary neoplasms -- Benign proliferative lesions of the male breast -- Carcinoma of the male breast -- Breast tumors in children -- Benign mesenchymal neoplasms -- Sarcoma -- Lymphoid and hematopoietic neoplasms of the breast -- Pathologic effects of therapy -- Cutaneous neoplasms -- The pathology of axillary and intramammary lymph nodes -- Pathologic examination of breast and lymph node specimens, including sentinel lymph nodes -- Molecular classification and testing of breast carcinoma.
  • 2009From: Ovid
    Paul Peter Rosen.
    Anatomy and physiological morphology -- Abnormalities of mammary growth and development -- Inflammatory and reactive tumors -- Specific infections -- Papilloma and related benign tumors -- Myoepithelial neoplasms -- Adenosis and microglandular adenosis -- Fibroepithelial neoplasms -- Ductal hyperplasia: usual and atypical -- Precancerous breast disease: epidemiological, pathological, and clinical considerations -- Intraductal carcinoma -- Invasive duct carcinoma: assessment of prognosis, morphologic prognostic markers, and tumor growth rate -- Tubular carcinoma -- Papillary carcinoma -- Medullary carcinoma -- Carcinoma with metaplasia -- Squamous carcinoma -- Mucinous carcinoma -- Apocrine carcinoma -- Mammary carcinomas with endocrine features -- Small cell (oat cell) carcinoma -- Secretory carcinoma -- Mammary carcinoma with osteoclast-like giant cells -- Cystic hypersecretory carcinoma and cystic hypersecretory hyperplasia -- Adenoid cystic carcinoma -- Cribriform carcinoma -- Lipid-rich carcinoma -- Glycogen-rich carcinoma -- Invasive micropapillary carcinoma -- Paget's disease of the nipple -- Lobular carcinoma in situ and atypical lobular hyperplasia -- Invasive lobular carcinoma -- Unusual clinical presentations of carcinoma -- Metastases in the breast from nonmammary malignant neoplasms -- Benign proliferative lesions of the male breast -- Carcinoma of the male breast -- Breast tumors of children -- Benign mesenchymal neoplasms -- Sarcoma -- Lymphoid and hematopoietic tumors -- Pathologic effects of therapy -- Cutaneous neoplasms -- The pathology of axillary and intramammary lymph nodes -- Pathologic examination of breast and lymph node specimens, including sentinel lympth nodes.
  • 2018From: ClinicalKey
    editor-in-chief, Ron M. Walls; senior editors, Robert S. Hockberger, Marianne Gausche-Hill.
  • 2007From: Springer
    Vincenzo R. Sanguineti.
  • 2016From: Wiley
    edited by Toby L. Simon, Jeffrey McCullough, Edward L. Snyder, Bjarte G. Solheim, Ronald G. Strauss.
    Sect. I: Contemporary issues in donation and transfusion -- Sect. II: Blood components and derivatives -- Pt. I: Red blood cells -- Pt. II: Platelets -- Pt. III: White blood cells -- Pt. IV: Plasma -- Sect. III: Apheresis, transplantation, and new therapies -- Sect. IV: Specialized clinical practice -- Pt. I: Obstetrics and pediatrics -- Pt. II: Surgery and trauma -- Pt. III: Oncology -- Sect. V: Hazards of transfusion -- Pt. I: Infection hazards -- Pt. II: Other hazards.
  • 2012From: Karger
    volume editor, Nicola Maffulli.
    Epidemiology, genetics, and biological factors of rotator cuff tears / Longo, U.G. ... [et al.] -- Biomechanics of the rotator cuff : European perspective / Longo, U.G. ... [et al.] -- Role of biomechanics in rotator cuff pathology : North American perspective / Karas, V.; Cole, B.J.; Wang, V.M. -- The scapula in rotator cuff disease / Ben Kibler, W. -- Instability and rotator cuff tear / Porcellini, G. ... [et al.] -- Growth factors and tendon healing / Oliva, F. ... [et al.] -- Autologous blood products in rotator cuff repair / Mei-Dan, O.; Carmont, M.R. -- Tendinopathy of the long head of the biceps / Snyder, G.M.; Mair, S.D.; Lattermann, C. -- Conservative treatment and rotator cuff tear progression / Longo, U.G. ... [et al.] -- Operative management of partial- and full-thickness rotator cuff tears -- Franceschi, F. ... [et al.] -- Subscapularis tears / Longo, U.G. ... [et al.] -- Single- and double-row repair for rotator cuff tears -- biology and mechanics / Papalia, R. ... [et al.] -- Arthroscopic transosseous rotator cuff repair / Longo, U.G. ... [et al.] -- Treatment options for chronic retracted degenerative rotator cuff tears / Deering, S.J.; Mair, S.D.; Lattermann, C. -- Synthetic augmentation in massive rotator cuff tears / Longo, U.G. ... [et al.] -- Understanding and preventing complications in repairing rotator cuff tears / Osti, L. ... [et al.].
  • 2000From: Springer Protocols
    edited by James Gray, Ulrich Desselberger.
    Rotaviruses / Ulrich Desselberger -- Electron cryomicroscopy and computer image processing techniques / B.V. Venkateram Prasad -- Virus replication / John T. Patton -- Rotavirus entry into tissue culture cells / Joanna M. Gilbert -- Mixed infections with rotaviruses / Robert E. Ramig -- Pathogenesis and animal models / Linda J. Saif -- Immunologic methods and correlates of protection / Kristine K. Mccartney -- In vitro study of immunity to rotaviruses / Manuel A. Franco -- Evaluation of rotavirus vaccines in small animal models / Max Ciarlet -- Methods of rotavirus detection, sero- and genotyping, sequencing, and phylogenetic analysis / Miren Iturriza Gómara -- Epidemiology of group A retrovisues / Mary Ramsay -- Future rotavirus research / Ulrich Desselberger.
    Also available: Print – 2000
  • v. 1-2, 2011.From: ClinicalKey
    [edited by] Harry N. Herkowitz, Steven R. Garfin, Frank J. Eismont, Gordon R. Bell, Richard A. Balderston.
    v. 1. Basic science -- Diagnosis -- Surgical anatomy and approaches -- The child's spine -- Arthritis and inflammatory disorders -- Cervical degenerative disorders -- Thoracic and lumbar disc disease -- Minimally invasive surgery -- v. 2. Spinal stenosis -- Spinal fusion and instrumentation -- Adult deformity -- Spine trauma -- Afflictions of the vertebrae -- Spinal cord -- Complications -- Failed surgery -- Chronic pain/rehabilitation.
    Also available: Print – v. 1-2, 2011.
  • 2016From: ClinicalKey
    Lara V. Marcuse, Madeline C. Fields, Jiyeoun (Jenna) Yoo ; foreward, Jacqueline A. French.
    Origin and technical aspects of the EEG -- The normal adult EEG -- The normal EEG from neonates to adolescents -- The abnormal EEG -- The EEG and epilepsy -- The EEG in other neurological and medical conditions and in status epilepticus -- The EEG : tips on indications, reading, and reporting -- Influence of common drugs on the EEG and on seizure threshold -- Treatment of status epilepticus -- Answers.
  • 2008From: ClinicalKey
    edited by F. Hampton Roy, Frederick W. Fraunfelder, Frederick T. Fraunfelder.
    Infectious diseases -- Parasitic diseases -- Nutritional diseases -- Disorders of protein metabolism -- Disorders of carbohydrate metabolism -- Disorders of lipid metabolism -- Hematologic and cardiovascular disorders -- Dermatologic disorders -- Connective tissue disorders -- Skeletal disorders -- Phakomatoses -- Neurologic disorders -- Neoplasms -- Mechanical and nonmechanical injuries -- Unclassified diseases or conditions -- Anterior chamber -- Choroid -- Conjunctiva -- Cornea -- Extraocular muscles -- Eyelids -- Globe -- Intraocular pressure -- Iris and ciliary body -- Lacrimal system -- Lens -- Macula -- Optic nerve -- Orbit -- Retina -- Sclera -- Vitreous.
  • 2007From: ScienceDirect
    edited by Jangu Banatvala and Catherine Peckham.
  • editors, Raphael Rubin, David S. Strayer ; consulting editor, Emanuel Rubin ; associate editors, Jay M. McDonald ... [et al.].
    PrintStatus: Not Checked OutLane Catalog Record
    Inflammation / Hedwig S. Murphy -- Repair, regeneration, and fibrosis / Gregory C. Sephel, Stephen C. Woodward -- Immunopathology / Jeffrey S. Warren, Douglas P. Bennett, Roger J. Pomerantz -- Neoplasia / Antonio Giordano ... [et al.] -- Developmental and genetic diseases / Anthony A. Killeen, Emanuel Rubin, David S. Strayer -- Hemodynamic disorders / Bruce M. McManus, Michael F. Allard, Robert Yanagawa -- Environmental and nutritional pathology / David S. Strayer, Emanuel Rubin -- Infectious and parasitic diseases / David A. Schwartz ... [et al.] -- Blood vessels / Avrum I. Gotlieb -- The heart / Jeffrey E. Saffitz -- The respiratory system / Mary Beth Beasley, William D. Travis, Emanuel Rubin -- The gastrointestinal tract / Frank A. Mitros, Emanuel Rubin -- The liver and biliary system / Raphael Rubin, Emanuel Rubin -- The pancreas / Gregory Y. Lauwers, Mari Mino-Kenudson, Raphael Rubin -- The kidney / J. Charles Jennette -- The lower urinary tract and male reproductive system / Ivan Damjanov -- The female reproductive system / Stanley J. Robboy, Maria J. Merino, George L. Mutter -- The breast / Ann D. Thor, Adeboye O. Osunkoya -- Hematopathology / Roland Schwarting, Steven McKenzie, Raphael Rubin -- The endocrine system / Maria Merino ... [et al.] -- Obesity, diabetes mellitus, metabolic syndrome / Barry J. Goldstein, Serge Jabbour, Kevin Furlong -- The amyloidoses / Robert Kisilevsky -- The skin / Craig A. Storm, David E. Elder -- The head and neck / Bruce M. Wenig -- Bones and joints / Benjamin L. Hoch, Michael J. Klein, Alan L. Schiller -- Skeletal muscle / Lawrence C. Kenyon, Mark T. Curtis -- The nervous system / John Q. Trojanowski, Lawrence Kenyon, Thomas W. Bouldin -- The eye / Gordon K. Klintworth -- Cytopathology / Hormoz Ehya, Marluce Bibbo.
  • David Flanagan and Yukihiro Matsumoto.
  • editors, Colin D. Rudolph, Abraham M. Rudolph ; coeditors, Margaret K. Hostetter, George Lister, Norman J. Siegel.
  • Mia Alyce Levy.
    The Rule-Based Response Assessment Framework is a goal-driven, context-driven, and knowledge-based temporal interpretation framework that automates response assessment over clinical data. The goal of this work is to enable consistent application of standardized response criteria for clinical trial data analysis, and to enable research and development of response criteria. The Rule-Based Response Assessment Framework defines the dimensions of response assessment including the knowledge and tasks required to apply response criteria to patient data. The Framework consists of several knowledge models and several sub-methods that make up the Response Assessment Method. The framework is implemented using ontologies and rules with the semantic web formalisms of OWL and SWRL respectively. The framework is applied to two response criteria in the oncology domain and one response criterion for rheumatoid arthritis. I demonstrate that the implementation of the Rule-Based Response Assessment Framework is extensible, reusable and enables consistent application of response criteria to patient data and comparison of response criteria within a clinical domain. Future research directions will incorporate the Rule-Based Response Assessment Framework into a larger informatics infrastructure for application and development of oncology response criteria.
  • 2009From: Springer
    by Edward B. Noffsinger.
  • 2006From: ProQuest Safari
    Matthias Kalle Dalheimer and Matt Welsh.
  • 2017From: Springer
    Yoram Groner, Yoshiaki Ito, Paul Liu, James C. Neil, Nancy A. Speck, Andre van Wijnen, editors.
    Also available: Print – 2017
  • 2011From: Springer
    Matthias W. Wichmann, David C. Borgstrom, Nadine R. Caron, Guy Maddern (editors).
    Incorporating and balancing advancing subspecialization is a significant challenge of modern surgery. The changes of surgical education and early subspecialization is a smaller spectrum of experience of graduating surgeons joining the rural workforce. Surgeons working in rural and remote hospitals, however, must be proficient in the great breadth of current surgical practice and face a number of challenges and demands that are specific to rural surgery. This textbook provides an update on the evidence and surgical techniques for the experienced rural surgeon and most importantly is a guideline for younger surgeons and surgical trainees joining the general surgical workforce in rural and remote areas around the world.
  • Marta Fischer ; mit einer Einleitung von Ortrun Riha und Marta Fischer.
    Includes 80 biobibliographies of German, Baltic and Russian personal physicians.
  • Alicia Carolina Gutierrez.
    A facile and 100% regioselective cycloisomerization--6-pi-cyclization method for obtaining pyridines is described. The unsaturated ketones and aldehydes derived from the cycloisomerization of primary and secondary propargyl diynols in the presence of CpRu(MeCN)3PF6 are converted to 1-azatrienes which in turn undergo a subsequent electrocyclization--dehydration to provide pyridines with excellent regiocontrol. The ruthenium-catalyzed cycloisomerization is a mild, atom-economical method for obtaining the requisite dienone and dienal substrates. The cycloisomerization--6-pi-cyclization sequence may be carried out in one pot or in two independent steps. Additionally, the azatriene cyclization may be carried out in 1-3 hours under microwave irradiation or, for more sensitive substrates, at lower temperatures for longer periods of time (6--24 hours at 90 °C). An atom-economical method for the convenient synthesis of tetrahydropyrans and tetrahydrofurans is also described. Enones and enals derived from the [IndRu(PPh3)2Cl]-catalyzed redox-isomerization of primary and secondary propargyl alcohols undergo a subsequent intramolecular conjugate addition to provide cyclic ethers in excellent yields. Lastly, we have developed complementary methods for the transition metal-catalyzed enyne cycloisomerizations of cyclic olefins. By using distinct ruthenium and palladium catalysts, decalins and 7,6-bicycles can be obtained with dichotomous stereochemical outcomes. The change in mechanism that accompanies the change in metal affords trans-fused 1,4-dienes with ruthenium and their cis-fused diastereomers under palladium catalysis. In the reactions under ruthenium catalysis, a coordinating group is required, and acts to direct the metal to the same side of the carbocycle, resulting in the observed trans diastereoselectivity. Subtle changes in the carbocyclic substrate led to the discovery of a heretofore-unobserved mechanistic pathway, providing bicyclic cycloisomerization products under palladium catalysis, tricyclic products under ruthenium catalysis in DMA, and a 1:1 mixture of the two under ruthenium catalysis in acetone. The different coordination requirements of the two paths allow for the reaction to be shuttled through the metallacycle pathway (generating tricyclic products) when DMA is used as a solvent. This method of obtaining these cyclobutene products complements the existing methods for catalyzing the [2+2] cycloaddition of alkynes. While other methods have been demonstrated for larger ring sizes with palladium, and for specific alkyne termini with platinum and gold, this method is unique in its substrate scope. To the best of our knowledge, this is the first example of a [2+2] cycloaddition catalyzed by CpRu(MeCN)3PF6.
  • v.1-2=, 2014From: ClinicalKey
    [edited by] Jack L. Cronenwett, K. Wayne Johnston.
    Published in association with the Society for Vascular Surgery, Rutherford's Vascular Surgery presents state-of-the-art updates on all aspects of vascular health care. Extensively revised by many new authors to meet the needs of surgeons, interventionalists, and vascular medicine specialists, this medical reference book incorporates medical, endovascular and surgical treatment, as well as diagnostic techniques, decision making and fundamental vascular biology. Master the latest developments, techniques, and approaches with thorough updates on endovascular applications, vascular access, imaging, non-operative management, and much more.View clinical and physical findings and operative techniques more vividly with a full-color layout and images.Get answers you can depend on. Rutherford's delivers the world's most trusted information on all major areas of vascular health care, is written by international experts, and includes up-to-date bibliographies and annotated recommended references.
  • 2011From: Springer
    Adam J. Brooks, Jon Clasper, Mark J. Midwinter, Timothy J. Hodgetts, Peter F. Mahoney, editors.
    Part 1. Personal Views -- A Personal View / James M. Ryan -- A Personal Experience / Damian Douglas Keene -- The War in West Philadelphia / John P. Pryor -- Part 2. Weapons, Blast and Ballistics -- How Guns Work / Mark Byers, Kerry Starkey and Peter F. Mahoney -- The Effects of Bullets / Donald H. Jenkins -- Bombs, Mines, and Fragmentation / Toney W. Baskin and John B. Holcomb -- Cluster Munitions / Henry Dowlen -- Shaped Charges and Explosively Formed Projectiles / Jonathan Morrison and Peter F. Mahoney -- Suicide Bombs / Piers R. J. Page and Ian Greaves -- Blast Injury / Emrys Kirkman, Neal Jacobs, Giles R. Nordmann, Stuart Harrisson and Peter F. Mahoney, et al. -- Ballistic Protection / Alan Hepper, Daniel Longhurst, Graham Cooper and Philip Gotts -- Forensic Aspects of Ballistic Injury / Jeanine Vellema and Hendrik Scholtz -- Part 3. Trauma Systems -- Civilian Trauma Systems / Jason Smith -- The UK Military Trauma System / Timothy J. Hodgetts -- Part 4. Prehospital and the Emergency Room -- Triage / Rob Russell -- Prehospital Care / Mark Byers, John-Joe Reilly and Peter F. Mahoney -- Emergency Department Management / Jason Smith -- Imaging Triage for Ballistic Trauma / Jon David Simmons and Tracy R. Bilski -- Part 5. Advanced Resuscitation and Anaesthesia, Section -- Damage Control Resuscitation / Adam J. Brooks and Bryan A. Cotton -- Battlefield Analgesia / Aaron D. Nelson and Dominic J. Aldington -- Anesthesia for the Ballistic Casualty / Craig C. McFarland -- Regional Anesthesia for the Ballistic Trauma Victim / Dan Connor -- Part 6. Clinical Care -- Damage Control Surgery: Concepts / Mark J. Midwinter and Adam J. Brooks -- Management of Ballistic Trauma to the Head / Geoffrey S. F. Ling, Chris J. Neal and James M. Ecklund -- Spinal Injury / Neil Buxton -- Eyes / Robert A. H. Scott -- Maxillofacial Ballistic Injuries / Andrew Martin Monaghan -- Neck Injury / Matthew J. Borkon and Bryan A. Cotton -- Damage Control / Benjamin Braslow, Adam J. Brooks and C. William Schwab -- Management of Vascular Trauma / Kate Brown and Nigel Tai -- Damage Control Part III: Definitive Reconstruction / Steven R. Allen, Adam J. Brooks, Patrick M. Reilly and Bryan A. Cotton -- Penetrating Genitourinary Trauma / Jay J. Doucet and David B. Hoyt -- Burns / Alan Kay -- Management of Extremity Injuries / David E. Hinsley and Jon Clasper -- Ballistic Trauma in Children / Graeme Pitcher -- Ballistic Trauma in Pregnancy / Michael J. Socher and Peter E. Nielsen -- Managing Ballistic Injury in the NGO Environment / Ari K. Leppäniemi -- Part 7. Critical Care -- Critical Care for Ballistic Trauma in Austere Environments / Christian B. Swift and Geoffrey S. F. Ling -- Transfer and Evacuation / Robert D. Tipping and Jonathan Vollam -- Critical Care Management: The Patient with Ballistic Trauma / Tim Nutbeam and Damian Douglas Keene -- Critical Care Nursing at Role Four / Clare Dutton -- The Role of Nutrition in the Treatments of Injured Military Personnel / Susan Duff, Susan Price and Jennifer Gray -- Part 8. Reconstruction & Rehabilitation -- Role 4 and Reconstruction / Steven L. A. Jeffery and Keith Porter -- Conflict Rehabilitation / John Etherington -- Part 9. And Finally -- Have You Read MASH? / Peter F. Mahoney.
  • v.1-3, 2018From: ClinicalKey
    editor-in-chief, Andrew P. Schachat.
    V. 1. Part 1: Retinal imaging and diagnostics / edited by Srinivas R. Sadda -- Part 2: Basic science and translation to therapy / edited by David R. Hinton -- v.2. Medical retina/ edited by Andrew P. Schachat and Srinivas R. Sadda -- v. 3. Part 1: Surgical retina / edited by C.P. Wilkinson and Peter Wiedemann -- Part 2: Tumors of the retina, choroid, and vitreous / edited by Andrew P. Schachat.
  • 2013From: ClinicalKey
    edited by SriniVas R. Sadda MD ; editor-in-chief, Stephen J. Ryan MD.
    Sect. 1. Optical Imaging Technologies. Fluorescein angiography : basic principles and interpretation / Robert N. Johnson, et al. -- Clinical applications of diagnostic indocyanine green angiography / Giovanni Staurenghi, Ferdinando Bottoni, and Andrea Giani -- Optical coherence tomography / Carlos Alexandre de Amorim Garcia Filho, et al. -- Autofluorescence imaging / Monika Fleckenstein, Steffen Schmitz-Valckenberg, and Frank G. Holz -- Advanced imaging technologies / Pearse A. Keane, Humberto Ruiz-Garcia, and SriniVas R. Sadda -- Image processing / Michael Abramoff and Christine N. Kay -- Sect. 2. Retinal Diagnostics. Electrogenesis of the electroretinogram / Laura J. Frishman -- Clinical Electrophysiology / Yozo Miyake and Kei Shinoda -- Diagnostic ophthalmic ultrasound / Rudolf F. Guthoff, Leanne T Labriola, and Oliver Stachs -- Color vision and night vision / Dingcai Cao -- Visual acuity and contrast sensitivity / Gary S. Rubin -- Visual fields in retinal disease / Rajeev S. Ramchandran and Steven E. Feldon.

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What is it?

Derived from Current Medical Diagnosis & Treatment, AccessMedicine's Quick Medical Diagnosis & Treatment provides topic reviews with key diagnostic and treatment features for more than 500 diseases.

A repository of medical knowledge from internal medicine, cardiology, genetics, pharmacy, diagnosis and management, basic sciences, patient care, and more.

Continuously expanding, all databases in the repository contain the latest editions of selected medical titles.

MicroMedex: Premier pharmaceutical information source containing multiple databases and drug reference tools. Of particular value is DRUGDEX Evaluations, one of the most comprehensive drug sources available.DynaMed Plus is a clinical information resource used to answer questions quickly at the point-of-care. Easy-to-interpret Levels of Evidence help clinicians rapidly determine the quality of the available evidence.

Biomedical and pharmacological abstracting and indexing database of published literature, by Elsevier. Embase® contains over 32 million records from over 8,500 currently published journals (1947-present) and is noteworthy for its extensive coverage of the international pharmaceutical and alternative/complementary medicine literature.

Scopus is the largest abstract and citation database of peer-reviewed literature: scientific journals, books and conference proceedings.A drug information resource containing: American Hospital Formulary System (AHFS), drug formulary for Lucile Packard Children's Hospital (LPCH) and Stanford Hospital & Clinics (SHC), Lexi-Drugs (adverse reactions, dosage and administration, mechanism of action, storage, use, and administration information), Lexi-Calc, Lexi-ID, Lexi-I.V. Compatibility (King Guide), Lexi-Interact, and Lexi-PALS.Cumulative Index to Nursing and Allied Health Literature (CINAHL) contains coverage of nursing and allied health literature.A knowledge database that provides access to topic reviews based on over 6000 clinically relevant articles. The evidence-based content, updated regularly, provides the latest practice guidelines in 59 medical specialties.Provides critical assessments of systematic reviews compiled from a variety of medical journals.Selects from the biomedical literature original studies and systematic reviews that are immediately clinically relevant and then summarizes these articles in an enhanced abstract with expert commentary.

Multidisciplinary coverage of over 10,000 high-impact journals in the sciences, social sciences, and arts and humanities, as well as international proceedings coverage for over 120,000 conferences.

Includes cited reference searching, citation maps, and an analyze tool.

Features systematic reviews that summarize the effects of interventions and makes a determination whether the intervention is efficacious or not.

Cochrane reviews are created through a strict process of compiling and analyzing data from multiple randomized control trials to ensure comprehensiveness and reliability.

Provides systematic coverage of the psychological literature from the 1800s to the present through articles, book chapters and dissertations.BMJ Clinical Evidence. A clinical information tool built around systematic reviews summarizing the current state of knowledge about prevention and treatment of clinical conditions.PIER (Physicians' Information and Education Resource) is a Web-based decision-support tool designed for rapid point-of-care delivery of up-to-date, evidence-based guidance for primary care physicians.Cochrane Central Register of Controlled Trials (CENTRAL) provides access to 300,000 controlled trials that have been identified the Cochrane Collaboration.Provides drug information targeted for patients.A continually updating drug monograph.The National Guideline Clearinghouse (NGC): A comprehensive database of evidence-based clinical practice guidelines and related documents.MedlinePlus: A repository of health information from the National Library of Medicine. Links are from trusted sites. No advertising, no endorsement of commercial companies or productsLPCH CareNotes via MicroMedex: Patient education handouts customized by LPCH clinical staffMicromedex Lab Advisor: Evidence based laboratory test informationA drug database organized by generic name, trade name and drug class.LPCH / Stanford Hospital Formulary.A goldmine of trusted consumer health information from the world's largest medical library.A trusted source of expert advice for and about kids, providing the information necessary to help patients and parents understand their unique needs.Provides patient handouts from the American Academy of Family Physician.Access to the Stanford Health Library for patients.Lane provides access to over 5,000 eBooks many of which provide helpful background material that will prepare you to better tackle primary literature.

Largest, broadest eBook package; covers all sciences, as well as technology (including software), medicine, and humanities.

In addition to covering Wiley and Springer, MyiLibrary is also the only provider for Oxford and Cambridge University Press titles. No seat restrictions.

A collection of biomedical books that can be searched directly by concept, and linked to terms in PubMed abstracts.

A web-based, decision support system for infectious diseases, epidemiology, microbiology and antimicrobial chemotherapy. The database, updated weekly, currently includes 337 diseases, 224 countries, 1,147 microbial taxa and 306 antibacterial (-fungal, -parasitic, -viral) agents and vaccines.

Over 10,000 notes outline the status of specific infections within each country.

Large number of high quality software and database programming titles from O'Reilly. Other software titles are also available from Sams and Prentice Hall. Limited to 7 concurrent users.Vast collection of software and database programming titles from multiple publishers, including Microsoft Press.Largest provider of engineering-related eBooks; includes titles in computer science and biomedical engineering.Over 4,000 full-text e-books covering scientific and technical information from CRC Press and others. Many handbooks and single volume reference sources.Includes peer-reviewed life science and biomedical research protocols compiled from Methods in Molecular Biology, Methods in Molecular Medicine, Methods in Biotechnology, Methods in Pharmacology and Toxicology, Neuromethods, the Biomethods Handbook, the Proteomics Handbook, and Springer Laboratory Manuals.Contains full text access to selected biomedical and nursing books.

Provides online, full-text access to Springer's journal titles as well as journals from other publishers.

Subjects include: life sciences, chemical sciences, environmental sciences, geosciences, computer science, mathematics, medicine, physics and astronomy, engineering and economics. Also includes eBooks.

Collection of over 8 thousand fulltext titles in engineering, math, and basic and applied biomedical research. Coverage is from 1967 to the present.A library of ebooks on a wide array of topics, digitized and made available online in conjunction with the original publishers.

Stanford Medicine

Lane Medical Library